LOCUS       DAVBCN010000001        83305 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVBCN010000001 DAVBCN010000000
VERSION     DAVBCN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 83305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83305
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.001"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            102..350
                     /locus_tag="VQ497_000001"
     CDS             102..350
                     /locus_tag="VQ497_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516376.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRIYSGNLILQELITVNGAYKATYIGELSSN"
     gene            481..1653
                     /locus_tag="VQ497_000002"
     CDS             481..1653
                     /locus_tag="VQ497_000002"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HET2516377.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENADSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            1653..2927
                     /locus_tag="VQ497_000003"
     CDS             1653..2927
                     /locus_tag="VQ497_000003"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HET2516378.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLEFANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLIGILSNYATDSL
                     VTASCRMAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VAFSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(2963..3928)
                     /locus_tag="VQ497_000004"
     CDS             complement(2963..3928)
                     /locus_tag="VQ497_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2516379.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQTIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(3909..5363)
                     /locus_tag="VQ497_000005"
     CDS             complement(3909..5363)
                     /locus_tag="VQ497_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET2516380.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKSQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEHPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            5740..7623
                     /locus_tag="VQ497_000006"
     CDS             5740..7623
                     /locus_tag="VQ497_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2516381.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYALADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVSKGDAVIE
                     VKI"
     gene            7772..8659
                     /locus_tag="VQ497_000007"
     CDS             7772..8659
                     /locus_tag="VQ497_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2516382.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGTSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(8979..9596)
                     /locus_tag="VQ497_000008"
     CDS             complement(8979..9596)
                     /locus_tag="VQ497_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HET2516383.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(10011..10199)
                     /locus_tag="VQ497_000009"
                     /pseudo
     CDS             complement(10011..10199)
                     /locus_tag="VQ497_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            10905..11798
                     /locus_tag="VQ497_000010"
     CDS             10905..11798
                     /locus_tag="VQ497_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2516384.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            11795..12994
                     /locus_tag="VQ497_000011"
     CDS             11795..12994
                     /locus_tag="VQ497_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2516385.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLEQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLDQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(13347..14114)
                     /locus_tag="VQ497_000012"
     CDS             complement(13347..14114)
                     /locus_tag="VQ497_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516386.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(14131..14826)
                     /locus_tag="VQ497_000013"
     CDS             complement(14131..14826)
                     /locus_tag="VQ497_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2516387.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            complement(14831..15196)
                     /locus_tag="VQ497_000014"
     CDS             complement(14831..15196)
                     /locus_tag="VQ497_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2516388.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMIH
                     FGYEKEELPGVVSDYIKGV"
     gene            15336..15809
                     /gene="nrdR"
                     /locus_tag="VQ497_000015"
     CDS             15336..15809
                     /gene="nrdR"
                     /locus_tag="VQ497_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HET2516389.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            15810..16979
                     /locus_tag="VQ497_000016"
     CDS             15810..16979
                     /locus_tag="VQ497_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HET2516390.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFNPKEATIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRER
                     GQKTKTQKQNQTAPAKTNIPKWSNPDYKNETSEETRLELERKKQELLARLEKGGD"
     gene            16980..17876
                     /gene="dnaI"
                     /locus_tag="VQ497_000017"
     CDS             16980..17876
                     /gene="dnaI"
                     /locus_tag="VQ497_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HET2516391.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            17873..18586
                     /locus_tag="VQ497_000018"
     CDS             17873..18586
                     /locus_tag="VQ497_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HET2516392.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            18600..19910
                     /gene="der"
                     /locus_tag="VQ497_000019"
     CDS             18600..19910
                     /gene="der"
                     /locus_tag="VQ497_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET2516393.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            20001..20336
                     /locus_tag="VQ497_000020"
     CDS             20001..20336
                     /locus_tag="VQ497_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516394.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            20476..20811
                     /locus_tag="VQ497_000021"
     CDS             20476..20811
                     /locus_tag="VQ497_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516395.1"
                     /translation="MEYLFKMIILLPCFYFFSWIEKANRDSKIFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGGRH"
     gene            20996..21787
                     /locus_tag="VQ497_000022"
     CDS             20996..21787
                     /locus_tag="VQ497_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516396.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLEWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYVVPYMVLKKYEDNV"
     gene            21780..22421
                     /locus_tag="VQ497_000023"
     CDS             21780..22421
                     /locus_tag="VQ497_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516397.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLIVSRLEEKFKKG"
     gene            22425..23075
                     /locus_tag="VQ497_000024"
     CDS             22425..23075
                     /locus_tag="VQ497_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2516398.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            23086..23373
                     /locus_tag="VQ497_000025"
     CDS             23086..23373
                     /locus_tag="VQ497_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516399.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     MQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            23519..26032
                     /gene="secA"
                     /locus_tag="VQ497_000026"
     CDS             23519..26032
                     /gene="secA"
                     /locus_tag="VQ497_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET2516400.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMATLTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQAMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            26113..27144
                     /locus_tag="VQ497_000027"
     CDS             26113..27144
                     /locus_tag="VQ497_000027"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET2516401.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            27146..28177
                     /locus_tag="VQ497_000028"
     CDS             27146..28177
                     /locus_tag="VQ497_000028"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET2516402.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            28216..28584
                     /locus_tag="VQ497_000029"
     CDS             28216..28584
                     /locus_tag="VQ497_000029"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET2516403.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            28574..29677
                     /locus_tag="VQ497_000030"
     CDS             28574..29677
                     /locus_tag="VQ497_000030"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET2516404.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLALPYDLIPALTLESALVHIKTVPAGACMGYGATYQTDSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            29696..31711
                     /gene="recG"
                     /locus_tag="VQ497_000031"
     CDS             29696..31711
                     /gene="recG"
                     /locus_tag="VQ497_000031"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET2516405.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     IKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            32027..33007
                     /locus_tag="VQ497_000032"
     CDS             32027..33007
                     /locus_tag="VQ497_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HET2516406.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            33812..34039
                     /locus_tag="VQ497_000033"
     CDS             33812..34039
                     /locus_tag="VQ497_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516407.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            34327..37224
                     /gene="nanA"
                     /locus_tag="VQ497_000034"
     CDS             34327..37224
                     /gene="nanA"
                     /locus_tag="VQ497_000034"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HET2516408.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGNRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHNGKEYIILSNAGGPNRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKDLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
     gene            38237..38689
                     /locus_tag="VQ497_000035"
     CDS             38237..38689
                     /locus_tag="VQ497_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2516409.1"
                     /translation="MIITKISHLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            38708..40045
                     /locus_tag="VQ497_000036"
     CDS             38708..40045
                     /locus_tag="VQ497_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET2516410.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            40124..41008
                     /locus_tag="VQ497_000037"
     CDS             40124..41008
                     /locus_tag="VQ497_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2516411.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            41008..41841
                     /locus_tag="VQ497_000038"
     CDS             41008..41841
                     /locus_tag="VQ497_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2516412.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAVFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            41859..43952
                     /gene="nanB"
                     /locus_tag="VQ497_000039"
     CDS             41859..43952
                     /gene="nanB"
                     /locus_tag="VQ497_000039"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HET2516413.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSTIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     NETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSKVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            43964..45067
                     /locus_tag="VQ497_000040"
     CDS             43964..45067
                     /locus_tag="VQ497_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2516414.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            45241..45939
                     /locus_tag="VQ497_000041"
     CDS             45241..45939
                     /locus_tag="VQ497_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2516415.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            45958..47475
                     /locus_tag="VQ497_000042"
     CDS             45958..47475
                     /locus_tag="VQ497_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2516416.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            47600..48928
                     /locus_tag="VQ497_000043"
     CDS             47600..48928
                     /locus_tag="VQ497_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2516417.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            49180..50046
                     /locus_tag="VQ497_000044"
     CDS             49180..50046
                     /locus_tag="VQ497_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2516418.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            50062..50901
                     /locus_tag="VQ497_000045"
     CDS             50062..50901
                     /locus_tag="VQ497_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2516419.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            51173..51625
                     /locus_tag="VQ497_000046"
     CDS             51173..51625
                     /locus_tag="VQ497_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2516420.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQAENDQFEYHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            51722..52066
                     /locus_tag="VQ497_000047"
     CDS             51722..52066
                     /locus_tag="VQ497_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516421.1"
                     /translation="MVSSKHAARASFLDGQGITVDETSWIINGIVNALIGRYIKLGTY
                     AAKYGISIARSILSRAAATAAARVGLLTKISGWILRVAVNVADVYGNFANNIAAAWDA
                     YDKIPNNGRINF"
     gene            52350..52994
                     /locus_tag="VQ497_000048"
     CDS             52350..52994
                     /locus_tag="VQ497_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET2516422.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVISCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            53163..54080
                     /locus_tag="VQ497_000049"
     CDS             53163..54080
                     /locus_tag="VQ497_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET2516423.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLITDKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            54098..54982
                     /locus_tag="VQ497_000050"
     CDS             54098..54982
                     /locus_tag="VQ497_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2516424.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     HMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(54998..55849)
                     /locus_tag="VQ497_000051"
     CDS             complement(54998..55849)
                     /locus_tag="VQ497_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2516425.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKNSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            56049..58106
                     /gene="pbp2b"
                     /locus_tag="VQ497_000052"
     CDS             56049..58106
                     /gene="pbp2b"
                     /locus_tag="VQ497_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HET2516426.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKIVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            58117..58713
                     /gene="recR"
                     /locus_tag="VQ497_000053"
     CDS             58117..58713
                     /gene="recR"
                     /locus_tag="VQ497_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HET2516427.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVATMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            58889..59932
                     /locus_tag="VQ497_000054"
     CDS             58889..59932
                     /locus_tag="VQ497_000054"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET2516428.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            60016..61389
                     /locus_tag="VQ497_000055"
     CDS             60016..61389
                     /locus_tag="VQ497_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET2516429.1"
                     /translation="MKLTIHEVAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGVRDGHDFIERAFENGAAATLSEKEVANHPYILVDDVLTAFQQLAAYYLEKTAVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQVLDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGVQSVQLHNQMILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVE
                     SLENEDK"
     gene            61376..61987
                     /locus_tag="VQ497_000056"
     CDS             61376..61987
                     /locus_tag="VQ497_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2516430.1"
                     /translation="MKTSDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRDLLSEML
                     NQASDLDSEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDPLPNLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            62078..62782
                     /locus_tag="VQ497_000057"
     CDS             62078..62782
                     /locus_tag="VQ497_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HET2516431.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            63001..64374
                     /gene="ftsA"
                     /locus_tag="VQ497_000058"
     CDS             63001..64374
                     /gene="ftsA"
                     /locus_tag="VQ497_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET2516432.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENIIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVESVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            64391..65650
                     /gene="ftsZ"
                     /locus_tag="VQ497_000059"
     CDS             64391..65650
                     /gene="ftsZ"
                     /locus_tag="VQ497_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET2516433.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            65655..66326
                     /locus_tag="VQ497_000060"
     CDS             65655..66326
                     /locus_tag="VQ497_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET2516434.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKATQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            66336..66875
                     /locus_tag="VQ497_000061"
     CDS             66336..66875
                     /locus_tag="VQ497_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HET2516435.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            66875..67138
                     /locus_tag="VQ497_000062"
     CDS             66875..67138
                     /locus_tag="VQ497_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET2516436.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            67135..67920
                     /locus_tag="VQ497_000063"
     CDS             67135..67920
                     /locus_tag="VQ497_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET2516437.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            67929..68717
                     /gene="divIVA"
                     /locus_tag="VQ497_000064"
     CDS             67929..68717
                     /gene="divIVA"
                     /locus_tag="VQ497_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HET2516438.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            69065..71857
                     /locus_tag="VQ497_000065"
     CDS             69065..71857
                     /locus_tag="VQ497_000065"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET2516439.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYANFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEEPAPEAALSFEDVVFTVERAAGEVCDRCRRIDPTTAERSYHAVICDHCASI
                     VEENFADAVAEGFEEK"
     gene            72267..72629
                     /locus_tag="VQ497_000066"
     CDS             72267..72629
                     /locus_tag="VQ497_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516440.1"
                     /translation="MKSSGIVGLVGGILLLICPFVLLGTAVNTAATTLNGGATAGAFS
                     GVALLLNALKIANLVLGIIAIVYYKGDKHVGAAPSVLMIVSGEVSLIPFLGWVGGILA
                     IIGGSLFLATLKKFKSEE"
     gene            73164..73856
                     /locus_tag="VQ497_000067"
     CDS             73164..73856
                     /locus_tag="VQ497_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HET2516441.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            73909..76320
                     /locus_tag="VQ497_000068"
     CDS             73909..76320
                     /locus_tag="VQ497_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HET2516442.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     RKAFASFPDDLLIQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYSGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            76570..77271
                     /locus_tag="VQ497_000069"
     CDS             76570..77271
                     /locus_tag="VQ497_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2516443.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIQMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            77273..79973
                     /locus_tag="VQ497_000070"
                     /pseudo
     CDS             77273..79973
                     /locus_tag="VQ497_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
     gene            complement(80069..80560)
                     /gene="tpx"
                     /locus_tag="VQ497_000071"
     CDS             complement(80069..80560)
                     /gene="tpx"
                     /locus_tag="VQ497_000071"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HET2516444.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(80683..81612)
                     /gene="psaA"
                     /locus_tag="VQ497_000072"
     CDS             complement(80683..81612)
                     /gene="psaA"
                     /locus_tag="VQ497_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HET2516445.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(81644..82492)
                     /locus_tag="VQ497_000073"
     CDS             complement(81644..82492)
                     /locus_tag="VQ497_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET2516446.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(82489..83211)
                     /locus_tag="VQ497_000074"
     CDS             complement(82489..83211)
                     /locus_tag="VQ497_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET2516447.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT        25177 a        15180 c        18399 g        24549 t
ORIGIN      
        1 tgctggcatt atcaatcatg aactaggatt ctagttttgc aggaagtcta ctactttcct
       61 tattgtctgt aaatctactg accttgttgt ttatcccagt catggtttct agttcgggct
      121 cagagtttca aagtggatgg caagagcatc aattgattgc tgagaaggtt agtaaaacac
      181 ttgataagac atttgataag gatgtcagag aaattccgac cagtcagttt tatcaaaaat
      241 ttgtagatga gatgggaagg atttactcag gaaatttgat cctccaggag ctgataactg
      301 tgaatggagc ttataaagct acttatatcg gtgaactctc tagcaactaa tcattcttta
      361 aaatgatgac caataagagg cttgatttgg tgaatcaagt cttttttctc tcttttactg
      421 ccatttgtga taaaatagta tagaacgatt ttttacatga atgataaaac agaggtaaat
      481 atgacaatcg gtattgataa gattggtttt gcgaccagtc aatatgtctt gaaattacaa
      541 gacttagcag aagcgagggg aattgaccct gaaaaattaa gtaaaggact cttactcaag
      601 gaattgagta ttgcgcccct aactgaggac atcgtgacct tggcggccag tgctagtgac
      661 tctattttaa ctgagcaaga aagacaagaa gttgacatgg tcattgtggc taccgagtca
      721 ggaattgacc agagtaaggc tgcggccgtc tttgtgcatg gcttgctggg catccagccc
      781 tttgctcgta gtttcgagat taaagaagcc tgctatgggg cgacagctgc cctccattat
      841 gccaaattgc atgtggaaaa ttctccggag tccaaggtct tggtcattgc cagtgatatt
      901 gccaaatatg gtattgaaac tccgggagaa ccaactcaag gtgctggaag tgtagctatg
      961 ttgattacac aaaatccacg catgatggcc tttaataatg acaatgtagc tcagacccgt
     1021 gacatcatgg atttctggcg accaaattac tcgacaactc cttatgtaaa tggtgtctat
     1081 tctacccaac aatacttgga tagtttgaaa acgacttggc ttgaatatca aaaacgctac
     1141 cagcttactc tggatgattt tgcggctgtt tgtttccact tgccttatcc taaattagcg
     1201 ctaaaaggct tgaaaaaaat catggataag agcctgcctc aagagaaaaa agacctctta
     1261 caaaagcatt ttgaccagtc tattctctac agtcaaaagg tggggaatat ctacacaggt
     1321 tcacttttcc ttggactttt gtctctcttg gaaaatgcag atagcttgaa agctggggat
     1381 aaaatcgccc tttatagtta cggaagtgga gctgtggctg agttcttcag tggtgaattg
     1441 gttgaaggat atgaagctta tttggataaa gaccgcttga acaagctcaa ccaacgaact
     1501 gccttatccg ttgcagacta tgaaaaggtc ttttttgagg aagtaaactt ggatgaaaca
     1561 aactctgccc agtttgctgg ctatgaaaat caagattttg ccttggttga aattctcgac
     1621 caccaacgcc gttatagcaa ggttgaaaaa taatgaagat aagttggaat ggattttcta
     1681 aaaaatcata ccaagagcgc ctcgagctgt taaaagctca ggcgctcctt agtcctgaga
     1741 gacaagctag tctggagaag gatgaacaga tgagtgtgac tgtggcagac cagctgagtg
     1801 agaatgtagt gggaactttt tctctgcctt attcgctggt tccggaggta cttgtcaacg
     1861 gtcaggaata caccgttccc tatgtgacag aagaaccctc tgtggttgcg gcggccagct
     1921 atgccagcaa aatcatcaag cgtgcaggtg gttttactgc acaagtccat cagcgccaga
     1981 tgattgggca ggtagccctt tatcaagttg ctaatcctaa actagcgcaa gagaagattg
     2041 ccagcaagaa agcggagctc ttggagtttg ccaatcaagc ctatccttct atcgttaaac
     2101 gtggaggtgg ggcgcgtgat ctgcatgtcg agcagataaa aggcgaacca gactttctcg
     2161 ttgtttatat tcatgtcgat acccaggaag ccatgggtgc caatatgctc aacaccatgc
     2221 tggaagcctt gaaaccagtc ttagaagaac tcagtcaggg acagagtctc ataggaatcc
     2281 tgtccaacta cgcgaccgat tctctggtga ctgcaagctg tcgcatggcc tttcgctact
     2341 tgagccgcca aaaggatcaa ggacgagaga ttgcggagaa aattgcgttg gctagtcagt
     2401 ttgcgcaggc tgatccttac cgagctgcta ctcataataa aggaattttt aatggtattg
     2461 atgcgatttt gattgccact ggtaatgact ggcgtgccat cgaagctggg gcccatgcct
     2521 ttgccagtcg agatggacgc tatcaaggtc ttagttgctg gacgctggac cttgaaagag
     2581 aagaattggt cggtgagatg accctgccca tgcctgtagc gactaagggt ggctctatcg
     2641 gcctcaaccc acgtgtagct ttcagtcatg atctactagg aaatccttct gccagagaat
     2701 tagcccagat tatcgtgtcc atcggtcttg cccaaaattt tgcagccctc aaagctttgg
     2761 taagtacggg catccagcaa ggccacatga aactacaggc caaatcccta gctctcctag
     2821 ctggggctag tgaatctgaa gttgctcccc tagtagagcg cctcatctca gataaaacct
     2881 ttaacctaga gacagcccag cgctatctcg aaaatttaag atcataaaaa actcagacga
     2941 atcggtctga gttttcttgt gtttaaatac tttttcctgg taatagtgta actggtaaga
     3001 agtaaccagt tgtggcaact tccttgcctt caatcttttg caagagaaga tcaatagtga
     3061 gacaagcaat ctcttccaaa ggttgcttga tagtagccaa ttgagggtag taattttcga
     3121 taaagtaggt cccatcatag ccgatgacct tgagctcttt tgggacagaa atgcccaatt
     3181 cttgagcgat tttaatgacc agaatagctg tcaaatcatc cgaagcaaaa atggcatctg
     3241 gtttttcccg ggtcaagata ttcttgattt ccatttcttt tctgacggga gaaaagtcac
     3301 tggaaacatt gataatagga gcttttggga gtacggatgc aaaaccagcg tggcgtagtc
     3361 cggttggcga attggaattg tcattccctg taatcatgat gatagtctgg gcacctgtct
     3421 tgaccaaggt ttgggcagca agaaccccac cagcatagtt gtcagaggag acgacaggga
     3481 tgtctggcga taggtttcgg tcaaaggaaa taatcggcgc tgtcacacga ttgtagtctt
     3541 cgattcctag gttgtgacta ccagaaatga tgccgtccac ctgattggct tccaacattt
     3601 cgatgtattc gcgttccttc tcagaatcat gttcactgtt gcagatgatg gtcttgtaac
     3661 catttttgaa gagttggtgt tccaatttat caatcaattc tgcatagaaa acattggaaa
     3721 tattagggaa aatcaagccg attaacttag ctgattttcc ttgcagacta cgagccaggt
     3781 tgttgggttt atagcccaat tctcgcatgg cttcattgac tttttggatg gttttctcag
     3841 atagataccc ttttttattg ataacccgag aaacggtagt aggactgacg cctgcaagtt
     3901 tggcgacatc agttagtttt gcgaccataa tctaattcat agtaagttcc agttgggttt
     3961 ccagatttga tcaggatacc attttggtcc gcatgtggga agacacgacc agaaaatact
     4021 ttttctcctt tattgatgaa aatttcaaag acagagttat cgatgaagat tgtagcagta
     4081 gtagcctgat tctcgatagg gcaagaacga gttgtcccaa attcttgggc atactgttct
     4141 ccagcctggc tacgatccac tgtcacttga ccgtttacaa ggtcaaagtt gattgaaagt
     4201 cccttacctt ctttatcagc aagtaagaca atctcgctct ggctattagc ttccaagttg
     4261 agttcaagtt cgtaagtgtt cttggtttgg gaacggtttg agaaggcttc ttcagaagca
     4321 cgaaggtcct taatagcagc gactggatac tggtagagct tgtcgtcttt gatagtgagt
     4381 tccttgacca aagagaaggt tccttggtgg tcaaaacggt cagatgggta agaaacatct
     4441 ggcaaaccaa gccagctaac tgctagagca cgcccatcag gagcgttgaa ggcttgagtt
     4501 gcataggctt cgaaaccgta atccatgttt tgaagttgag acacatctac cattttggca
     4561 tttttagggt caaaggaagc cccaatctta tacatatttg gaaagatatt atcgtagtct
     4621 agaactttct tatccaatcc ttgtggacag tagagaagga caggatgttc ctctacaaag
     4681 accagattag gacattccat catgtaggca gtacggtcgt tagcaaagtc aaggtcgcca
     4741 actgcttgcc agtttgtgta gtcgttattg acagccttgt agagacggac gaagcctttt
     4801 ttctccaagt cctgtccacc gacgacagca tagtattgac ttttaaagtt aaaaatttgt
     4861 ggatcgcgga agtggtcagt agagtctgct ggctggtcaa tcaagatctt gtcaatcttt
     4921 gtaatcttac cctccttgtc catcaaagca ccgatctggt atgggtgacg gatccagttt
     4981 tcatcgcgaa catttcctgt ataaaatagg aataagttat cgccaaattg catggcagaa
     5041 ccagagtagg caccgtggct atctaatgga gtatctggta aaactttgat tccagtttct
     5101 ttaaagtgaa ccaaatcatc actttctagc tgtgcccaag atttcaaacc gtgggctgca
     5161 ccaaaaggaa aattctggta aaagaggatc cacttgccat caaagtaaga aaagccattt
     5221 gggtcgttga gaagtcctgt ttttggctca acatggtaat gagtatgcca tggagattgt
     5281 gccatatttt cctttatatg ttgaatttct tcttgcgtcc aatcttgata aagtctgtaa
     5341 cgacgctcag ttgtccattc cattcattcc tccaaaaatc ttatcacttt taatagtaac
     5401 tgttttcgat aaaaaatgca agccttttat gttaaaaaag aggaaaaact gtcaaacgat
     5461 tatcataaaa taaatacagg aaatcaatca aatttttacg aaaatgtgaa agataatgaa
     5521 ataaaagact ttttaaccaa ggtttcaaac ttatttttca aggaattcct gatgaaacag
     5581 tcaaaaacaa tcaatctaaa acttgcccac taagtggata gaatattttt tctgcaaaac
     5641 gcttgacata tttttaaaac atgataaaat aatagtcgta agggcgaata aaatcgcttt
     5701 caaacaagaa caaaatgtta tataaggaga tttttgcaaa tgaacaatca ggaaattgca
     5761 aaaaaagtca tcgatgcctt gggcggacgt gaaaatgtca atagtgttgc ccactgtgcg
     5821 actcgtctac gtgtcatggt caaagatgaa gagaaaatca ataaagaagt gattgagaac
     5881 ttggaaaaag ttcaaggtgc tttctttaac tcagggcaat accaaattat ctttggtaca
     5941 ggtacagtta acaaaatgta cgatgaagtt gttgtacttg gcttaccaac atcatctaag
     6001 gatgacatga aagcagaagt tgctaaacaa gggaactggt tccaacgtgc tatccgtact
     6061 ttcggtgatg ttttcgttcc aatcatccca gttatcgtag cgacaggtct cttcatgggt
     6121 gtgcgtggtc ttttcaacgc tcttgaaatg ccacttccag gtgactttgc aacttacaca
     6181 caaatcttga cagatacagc cttcatcatc ttgccaggtt tggttgtgtg gtcaaccttc
     6241 cgtgtatttg gtggaaatcc tgccgttggt atcgttcttg gtatgatgct tgtctctggc
     6301 tcacttccaa acgcttgggc agttgctcaa ggtggtgaag taacagcgat gaacttcttt
     6361 ggtttcatcc ctgttgttgg tttgcaaggt tccgttcttc cagccttcat catcggggtt
     6421 gtcggagcta aatttgaaaa agctgtccgc aaggttgttc cagatgtcat tgacctcttg
     6481 gtaacaccat tcgtgacact tttggtcatg tctatccttg gactctttgt cattggacca
     6541 gttttccacg ttgttgaaaa ctacatcctt attgctacaa aagcgattct tagcatgcca
     6601 tttggtcttg gtggtttctt gattggtggg gttcaccaat tgatcgtcgt gtcaggtgtg
     6661 caccacatct tcaacttgct tgaagtgcaa ttacttgctg ctgaccatgc taacccattc
     6721 aacgctatca tcacagctgc tatgacagct caaggtgctg ctactgttgc ggttggtgtt
     6781 aaaacaaaaa atccaaaact gaaaacactt gctttcccgg ctgctctttc tgccttccta
     6841 ggtattacag agcctgctat cttcggggtg aacttgcgct tccgtaaacc attcttcctt
     6901 tcattgattg ctggtgcaat cggtggtgga ttggcttcta tccttggact tgctggtact
     6961 ggtaatggta tcaccatcat ccctggtaca atgctttatg ttggtaacgg acaacttcca
     7021 caataccttc ttatggtagc tgtatcattt gcccttggtt ttgctcttac ttacatgttt
     7081 ggttacgaag atgaagtaga cgcaactgca gctgcaaaac aagctgaagt ggctgaagaa
     7141 aaagaagaag ttgcgccagc agctcttcaa aatgaaacac ttgtaactcc tatcgtcggt
     7201 gatgttgtcg ctcttgctga tgtcaatgac ccagtcttct caagtggagc tatgggacaa
     7261 ggtatcgctg tgaaaccaag tcaaggcgtg gtctatgcac tagctgatgc tgaagtttca
     7321 attgcctttc caacagggca cgcttttggt ttgaaaacaa gaaatggtgc tgaagttttg
     7381 attcacgttg gtattgacac tgtatctatg aacggtgacg gttttgaagc aaaagttgct
     7441 caaggtaata aggtgaaagc tggcgatgtt cttggaacat ttgactcaaa caaaatcgct
     7501 gcagctggac ttgatgatac aacaatggtt atcgttacaa atacagctga ctacgcttca
     7561 gtagctccag tcgcaacagg ttcagtttcg aagggggatg ctgtgatcga agtgaaaatc
     7621 taatcaatcc tctctaatgt gaaaacgaac aaatgacatg tttgttcgtt tttattagat
     7681 agtaagattt acagaggaaa atctaaaaaa tagagacatt tagactttcg aagtatgcta
     7741 taataaagaa aataaaaaca agaggtttat catgacaaaa ttatatggaa gcttggaagc
     7801 gggcggtaca aagtttgtct gtgctgtcgg tgatgaaaac tttaacgttg tagaaaaaac
     7861 acaatttcca acaacaactc caatcgaaac aatcgataaa accattgagt tcttctcaaa
     7921 attcgataac cttgctggtc ttgcagttgg ttcatttggg ccgattgata ttgacaaaaa
     7981 ctcaaaaact tatggcttta tcacgacgac tccaaaacca aactgggcaa atgtggactt
     8041 gcttggtgcc cttcgtcgcg ccctaaacgt gccaatgtac ttcacaacag acgtaaacag
     8101 ctctgcttac ggtgaaatgg ttgctcgtaa caatgctggt ggtcgtatcg aaaacttggt
     8161 ttactacaca atcggtacag gtatcggtgc aggcgtcatc caacgtggtg agtttatcgg
     8221 tggtgtgggt caccctgaaa tgggtcatta ttatgttgct agacacccaa tggatattga
     8281 aaaagagttt aagggtgttt gtcctttcca taagggatgt ctagaaggtt atgcagctgg
     8341 tacaagtttg gaagctcgta caggtgtacg tggggaaact attgaactca acaaccctgt
     8401 ttgggatgtt caagcctact atatcgctca agctgcggtt aatgcgacag tgactttccg
     8461 cccagacgtg attgtctttg gtggaggggt catggctcaa caacatatgc tggaccgtgt
     8521 ccgtgagaaa tttacatctc ttcttaatgg ttacctacca gtaccagatg tgcgtgacta
     8581 tatcgtgacg ccagcagtcg caggaaatgg ttctgccaca cttgggaact ttgttcttgc
     8641 aaaagaagtt tcaaaataaa gcacaaaatc cgcttgggaa accaaacgga ttttgtggtt
     8701 gaaaacaata tttttgagcc tttccttaag tgattacgga cggtagcgac tttcttcgaa
     8761 attccatacc taaactttga gcctagtgtc tcaaagtttc cgaatacctg aaaccattag
     8821 atttcaggtg tttctatcac ggcggaaagg ctaggtaagt cctcgcctca ctatcgaaaa
     8881 atatgcataa tttccatacc aaagtatatt tagtatcaag ctatagttaa tctacgcttt
     8941 aaaaatccac ttgggaaacc aaacggattt tttacgtgtc aaagcatttg ccagaaaaag
     9001 tcaataatat aggtcagacc gtaggtataa accacgaggg taaagggttt ggtcagaatg
     9061 atgatagtca tgtagcgctt gaaactcatc ttggtcaggg cggccagcat acagagaaag
     9121 tcagctgggc taatgggcca aatcatcata aaaataaaga agcggtcaaa acgattgccc
     9181 ttatctagcc agccgatgta cttgtcgtag gtgcgcttgc tgacgacaga ctggacaaag
     9241 gcagctccgt ataggcgcac tagataaaag ataatggcac agccaatcac gatgccgata
     9301 tagttgtaga tagtcccgat gatgtgcccg tagataaaga ccccagccac cgaggtcaag
     9361 gcccctggaa tgatagggac gacagtctgt aaaatctgta aaaagataaa gagaggtgga
     9421 ccccagatgc ctgcctgctg gataaaggca gagagggttt ccttggattg taaaatccca
     9481 gcctgataag cccaaataca gaaaatcaaa ctcccaaccc caccgacaat cgatgagata
     9541 ttgataactc gctgcagaag gggagaaaca gctttcattt gtttatcctg tgacatgtaa
     9601 actcctcata gatagtatat ttctactata ccatattttg gaggagaaag ggggaatgag
     9661 tcttttttga taaggaatag aaagtaaata gcccttcgag acttagaata ggtctgtcaa
     9721 ctaaaggtta tatgcattct aggtttcgaa taatcgcaat tccataaatt taaaagtatt
     9781 ctaagtttcg aatagcttat ctgagataat tttgaggcat tcgcgctttc gaatagtcat
     9841 tttccctaaa gtttatatgt attctgaagc tagaaagact aaaatataaa agtagtaaga
     9901 gtctctctct gatggacgta atttatggct agtatgaatc ctttgaattg ttagttaatt
     9961 ttaatattaa tcagttctga aataattact aaatatagtt agatggtgag ttaattattg
    10021 ctaaatttca agttcaagtt agtcatccag aagtcttctc tgggggactt gtagttcaag
    10081 cattttttag gatagttgtt aatccacttt tcgataaatg cgacttcctt tgtagttgtt
    10141 ttcttagttc cctgaggtaa ccatctatga atgagcctgt tgtgattttc attagatcct
    10201 atagtgatgt gcataataga tatagcagat tgaaactaga atagtataca tctacttcta
    10261 aaacattgtt agaaatcaat ttgactgtcc tgatcaattt gtcctgtttt tatttcattt
    10321 tactatataa tccttagaaa acatatcgga caagcggttg aattccgttc cgtccattag
    10381 tagataaagg attactcctt cttgaaatga cagatgaact tgtttcttat aaaaatgttg
    10441 tctgaaacta ttagcctttt tccagaagtt ttagaggaag aaaactttac gaggaaaaag
    10501 gagttactca aataacaatt caggattaaa aatagacagt tgaggagcgg aaggtataaa
    10561 ttaagtttgc tactgtataa tggatttatc actaatactc ttcgaaaatc tcttcaaacc
    10621 acgtcagctt cgccttgccg tagatatagt tactgacttc gtcagttcta tccacaacct
    10681 caaaacagtg ttttgagctg acttcgtcag ttttatctgc aacctcaaag ctgtactttg
    10741 agcagcctac ggctagcttc ctagtttgct ctttgctttt cattgagtat aaaagtgctc
    10801 caggccatat tttacacagc aatagtcttc taaaaatgga caaaaacatt gtaaaggcta
    10861 tcaaaaagga gtataatgag tgcaagaaat ttcggaggtg aaagatgcta gaagtaagaa
    10921 gtctagagaa aagttttggc tccaagcaag ttttgtttgg tattgacttt caagcgcgac
    10981 caggtcgtat tttgggacta gtcggaaaaa atggtgctgg aaaaacaacg attttccata
    11041 gtattttgaa attcctagaa tatcagggag aaatcggtct ggatggtcag gatattcgtc
    11101 aggagaccta tgctcggatt ggttatctgc ctgaagaacg cagtctcatg cctaaattga
    11161 cagttcttga acaagttcgc tacttggcga ctctaaaagg catggatgcc aaagaagtca
    11221 aagaaaaact ccctcaatgg atgaagaggt tggaagtgaa aggaaagctg acagataaaa
    11281 tcaagagtct gtcaaaagga aatcagcaga agattcagct cattattact ctgattcatg
    11341 aaccagacct gattatcttg gatgagcctt ttagtggatt ggacccagtt aatacagaat
    11401 tgctcaaaca agtcattttt caggaaaaag agcgcggagc aaccattatc ttttctgacc
    11461 atgtcatgac caatgttgag gaactttgtg acgatattct gatgatccga gatggccgtg
    11521 tggtcttgca cggaccagtt caggatgtcc gcaatcaata cgggaaaacg cgtctctttg
    11581 tttcaagtga acgaagcaag gaagaattgg aaaatcttcc tcatgtcaaa caggtgagct
    11641 tgaccaaaca aggcagttgg aaattgattt tggaggatga gagcgctgga agggaactct
    11701 tcccaatctt gactcaaggt caatatatcg caacatttga ccaacaagcg ccaactatcg
    11761 atgaaatctt taaactagaa tcaggagtgg aagtatgaga aatatgtggg ttgtaatcaa
    11821 ggaaacctat cttcgacatg tcgagtcatg gagtttcttc tttatggtga tttcgccgtt
    11881 cctcttttta ggaatctctg taggaattgg gcatctccaa ggttcttcta tggctaaaaa
    11941 taataaagtg gcagtagtga caacagtgcc atctgtagca gaaggactga agaatgtaaa
    12001 tggtgttaac ttcgactata aagacgaagc aagtgccaaa gaagcaatta aagaagaaaa
    12061 attaaaaggt tatttgacca ttgatcaaga agatagtgtt ctaaaggcag tttatcatgg
    12121 cgaaacatcg cttgaaaatg gaattaaatt tgaggttaca ggtacactca atgaactgca
    12181 aaatcagctt aatcgttcaa ctgcttcctt gtctcaagag caggaaaaac gcttagagca
    12241 gacaattcaa ttcacagaaa agattgatga agccaaggaa aataaaaagt ttattcaaac
    12301 aattgcagca ggtgccttag gattctttct ttatatgatt ctgattacct atgcgggtgt
    12361 aacagctcag gaagttgcca gtgaaaaagg caccaaaatt atggaagtcg ttttttctag
    12421 cataagggca agtcactatt tctatgcgcg gatgatggct ctgtttctag tgattttaac
    12481 gcatattggg atctatgttg taggtggtct ggctgccgtt ttgctcttta aagatttgcc
    12541 attcttggat cagtctggta ttttggatca cttgggagat gctatctcac tgaatacctt
    12601 gctctttatt ttgatcagtc ttttcatgta cgtagtcttg gcagcattcc taggatctat
    12661 ggtttctcgt cctgaggact cagggaaagc cttgtcgcct ttgatgattt tgattatggg
    12721 tggttttttt ggagtgacag ctctaggtgc agctggtgac aatctcctct tgaagattgg
    12781 ttcttatatt ccctttattt cgaccttctt tatgccgttt cgaacgatta atggctatgc
    12841 ggggggagca gaagcatgga tttcacttgc tattacagtg atttttgcgg tggtagcaac
    12901 aggatttatc ggacgcatgt atgctagtct cgttcttcaa acggatgatt tagggatttg
    12961 gaaaaccttt aaacgtgcct tatcttataa atagaagagc cttgcgaaag cgaggttttt
    13021 aaaggtgaaa agggtggaat cacgaaccta atgatgtaca cattatgtaa acaggttgct
    13081 tgaaaactca attgtatagt gaactgaatc tggaatagga taatattact tctaaaacat
    13141 tcttataaat tgatttgaat tccaaaatcc catcgttccg ttcttatttc attttgcgat
    13201 atagtgaaag gaaataagtc agctcacaaa aaacgcataa tatcaaggat ttttaacact
    13261 tgatactatg cgttttctaa ttttaaagac tttttgcctg gtctcttttg ttctatgagt
    13321 atgttacata tctaggtaaa aattatttaa agcaaattaa ccttatttct caagatgtag
    13381 taggttccga gataaaagat agctatgaat atgagttctt taacaatggc tatacctgct
    13441 cccatataga aatggtcatt gagtcctacc agtgaattga catagaaatt agaactaaga
    13501 ttgaagaaaa gttcaataaa tccaatgacg atttggatac caatgtaggc tgcaacagcg
    13561 agtgctgtac ggtattcatt gaaaagctgt ccaatggaaa tagccaggta gatgcagagg
    13621 attcctgaaa tggtatttag taggaaggat atacctgtaa gaaagatctg agggagatgt
    13681 gtttctacaa atgtaatcac ataagaaaga ggaatccatt ctggagctgt taaagccaga
    13741 ataataacag cacttagagc caatacagcg gtgctaatca atgaccagat aaaggcaccg
    13801 attagtttgg ctgtgatgat atggtgttca gaaactggca aggtcaaagt cagatagcct
    13861 tgtcggtcgt agacactacc tttgaagcgt ttaataatca agaaaatggt tgaaatcgca
    13921 agtgtaagca tcaagccacc aaagactgta gctagaaaaa gtagcatagt agcctgactt
    13981 tcttggtaag gtagattttt aaagccttgt gtctgtattc cgatgagggc agaaaggact
    14041 agcacggctg cgtagagggc taaataccac ttgttaacat ttttaaattc gtagtgaact
    14101 aaattccaaa acataaataa tctcctttgc ttaggcctta aattcctgac ggaagagttg
    14161 gtcaatggat tcacctgact cgtagcgaat atcatctaca tttccttgac ggacgacttt
    14221 tccgtctttt aggaagacaa tttcatccaa gattggctcg atatcagaaa tcaagtgggt
    14281 agaaatcaaa acggtagaag ttggtgagta gttgttgata atggtattga ggatataagc
    14341 acgggctgct ggatccaccc caccaatggg ttcatccaaa acatagagac gagcatcacg
    14401 gctcataacc aaaatcagtt gaaccttttc tttgtttcct tttgatagtt tcttgagacg
    14461 actattttca tcaatgccca ggtctgcaag tagatgatga gcgcgttcaa gattgaaatc
    14521 tttatagaag gtcttgaagt aggttagggc ttctttgacc ttcatttgct cattgagata
    14581 ggtcgtatca ggcaaataag ctacaacggc cttggttgct gggcttgggt ccatgtcgtt
    14641 gatgaggaca cgtccttgat ctggttgtaa gaggccatta attagtttaa tcagggttgt
    14701 ttttcctgag ccgtttggcc caagaaggcc gacaattttt ccagctggaa tgtcaagaga
    14761 aacattttca agggctggtg ttgctccata tgatttggat acattttcaa atactagtaa
    14821 tgacataggc ttaaactcct ttaatataat cactgactac gcctggtagt tcttcttttt
    14881 catagccaaa atggatcatg gaggaaacga agtgttccaa ttcttcttct gataattgtt
    14941 tgcgtgactg agcgattagc tccttatcct tagtcacaaa tcgtccagtt gttcgcttgc
    15001 tgtagacaaa tccttctcgt tcaaggtctg ataaggctct ttggatggta ttgggattga
    15061 caccagcctc gctagctagc tccctcacgg ttggaagttg ttgattgggt tccagtgtat
    15121 gggaaacaat ctgaagcttg attttctcca taatctgtaa atagatgggt tttttgttgt
    15181 caaatgtcca ggacatctta gtctcctttc tcttatcctt ttgtcttaac taaataatac
    15241 aacaaaacaa aaaacttgtc aagcaaaaag aagaattgtt ttgggaatcg cttaaaaaat
    15301 ggtataatga aaagaaaacg ataaggaggg aaaggatgcg ttgtccaaaa tgtggggcta
    15361 ccaagtcaag tgttatcgat agtcgccaag cagaagaagg gaacaccatt cgtagaagac
    15421 gtgagtgcga cgaatgccaa caccgtttta caacctacga acgagtagaa gaaagaacct
    15481 tagtggttgt taaaaaagat ggtacacggg aacaattctc cagagataaa atctttaatg
    15541 ggattatccg ctcagcccag aaacgtcctg tgtcaagtga tgaaatcaac atggtagtca
    15601 atcgtatcga acagaaactc cgtggtcgaa atgaaaatga aattcaaagt gaggacattg
    15661 gttcactcgt catggaggag ttggctgaat tggacgagat tacctatgta cgttttgcta
    15721 gtgtctatcg tagttttaag gatgtcagtg agttagagag cttgctccaa caaatcaccc
    15781 agtcctctaa aaagaaaaag gaaagataaa tgaagccaat tgaccgtttt tcttatctaa
    15841 agaataatcg ggtgtcgcaa gatacctcat ctctggtaca gtgctacctc ccgattatcg
    15901 gtcaggaggc actgagcctt tatctttata caatcagttt ttgggataat ggtagaaagg
    15961 aatatctttt ttcaagtatc ctcaatcatc tcaactttgg gatggataga ctgataaaat
    16021 cattgaaaat cttatctgct tttaatctct tgaccctcta tcaaaagggg gatgtttatc
    16081 agctagccct ccatgctcct ctatctagtc aagacttctt ggagcatcct gtttatcgca
    16141 gactcttaga gaaaaagatt ggcgactctg ctgtggagga tttgaaagtt gaaagtgctg
    16201 atggtgaaga aatacctgtc tcactcaatc aagtctttcc agaattggca gaactaggca
    16261 gtcaagaaga ccttggtctc aagaagaaag tggccaatga ttttgacttg gaacattttc
    16321 gccagctgat ggctcgagat ggacttcgtt ttgcggatga gcagtccgat gtcttaaacc
    16381 tctttgctat tgccgaggag aagaaatgga cttggtttga aacctatcaa ttggccaagt
    16441 caacagctgt ttctcaggtt atttcaacca aacgcatgcg tgaaaaaatt gctcaaaaac
    16501 cagtttcctc tgactttaat cctaaggaag caaccattat caaggaagct aaaagtaaga
    16561 ctgccctgca gttcttagca gaaatcaagc aaacacgcaa ggggaccatt acccaaacag
    16621 aaagagaact tttgcaacag atggctggct tgggcttgct ggacgaagtc atcaatatca
    16681 ttctcttgtt gacctttaat aaggtggatt cggcaaacat caatgagaaa tatgccatga
    16741 aggtggctaa tgactatgcc tatcaaaaga tacattcagc agaagaggcc gttttacgaa
    16801 ttcgtgagcg agggcagaaa acaaaaacac aaaaacagaa tcagactgcc ccagcaaaaa
    16861 ccaatattcc taaatggagt aacccagatt ataagaatga aaccagcgag gaaactcgtc
    16921 tggaactaga acgtaagaaa caagaactat tagctcgatt agaaaaagga ggagattaga
    16981 tggaaagtgt cggagacgta ctcaaacgtc aacccagccg tttttattat caagatttgg
    17041 tccagaaaat catgaaggac cctgatgttg cggcttttat ccagcaagaa tccttgactc
    17101 cagaggaatt aaatcgcagt atctccaagt ttaatcagta catcaccgag cgtgacaagt
    17161 ttctccgtgg ggatacggat tatattgcca aaggctacaa gccgattttg gttaagaatc
    17221 atggctatgc agatgtttca tatgaagaaa ctcctgagct aatcgcggct gaaaaagaag
    17281 cggctattaa gaatcgtctc aagctaatca atctaccagc cagtctcaaa aaagctagtt
    17341 tggctcaagt tgatttggat gatttgggac gcttgccagt ttttgaaaaa ctattagcct
    17401 ttgtggagca atatccagct attcgaaaag gtctttactt atatggagac tttggtgtgg
    17461 gtaaaagttt catggtggcg gccttagctc atgatttatc agaaaaacgt ggtgtttcat
    17521 caactctcct ccactatcct agctttgtca ttgatgtcaa aaatgctatc agtgatggca
    17581 atgttaagac cttggtggat gagattaagc tgtctgaggt cctgatttta gatgatattg
    17641 gcgccgagca atcaaccgct tgggtgcgtg acgaaatcct gcaggtcatt ctccaatatc
    17701 ggatgcagga aaatttaccg acctttttca catccaactt caactttgaa gatttggaga
    17761 agcattttgc gaaagtgaaa catggaaatg acgaaacctg ggaagccaga cgggtcatgg
    17821 aacgcatccg ttatttggct gaggagactc gtttagaagg agtaaaccgt cgatgacaga
    17881 aaccattaaa ttgatgaagg ctcatacttc agtgcgcagg tttaaagagc aagaaattcc
    17941 ccaagtagac ttaaatgaga ttttgacagc agcccagatg gcatcatctt ggaagaattt
    18001 ccaatcctac tctgtgattg tggtacgaag tcaagagaag aaagatgcct tgtatgaatt
    18061 ggtacctcaa gaagccattc gccagtctgc tgttttcctt ctctttgtcg gagatttgaa
    18121 ccgagcagaa aagggagccc gacttcatac cgacaccttc caaccccaag gtgtggaagg
    18181 tctcttgatt agttcggtcg atgcagctct tgctggacaa aacgccttgt tggcagctga
    18241 aagcttgggc tatggtggtg tgattatcgg tttggttcga tacaagtctg aagaagtggc
    18301 agagctcttt aacctacctg actacaccta ttctgtcttt gggatggcac tgggtgtgcc
    18361 aaatcaacat catgatatga aaccgagact gccactagag aatgttgtct ttgaggaaga
    18421 ataccaagaa cagtcaactg aggcaatcca agcttatgac cgtgttcagg ctgactatgc
    18481 tggggcgcgt gcgaccacaa gctggagtca gcgcctagca gaacagtttg gtcaagctga
    18541 accaagctca actagaaaaa atcttgaaca gaagaaatta ttgtagaaag tgagaaatta
    18601 tggccctacc aactattgcc attgtaggac gtcccaatgt tgggaaatca accctattta
    18661 atcggatcgc tggtgagcga atctccattg tagaagatgt cgaaggagtg acacgtgacc
    18721 gtatttatgc aacgggtgag tggctcaatc gttcttttag catgattgat acaggaggaa
    18781 ttgatgatgt cgatgctcct ttcatggaac aaatcaagca ccaggcagaa attgccatgg
    18841 aagaagcaga tgttatcgtc tttgtcgtgt ccggtaagga aggaattacc gatgcagacg
    18901 aatacgtagc ccgtaagctt tataagaccc acaaaccagt tatcctcgca gtcaacaagg
    18961 tggacaaccc tgagatgaga aatgatatct atgatttcta tgctctcggt ttgggtgaac
    19021 cattgcctat ctcatctgtc catggaatcg gtacagggga tgtgctagat gcgatcgtag
    19081 aaaatcttcc aaatgaatat gaggaagaaa atccagatgt cattaagttt agcttgattg
    19141 gtcgtcctaa cgttggaaaa tcaagcttga tcaatgctat cttgggagaa gaccgtgtta
    19201 ttgctagtcc tgttgctgga acaactcgtg atgccattga tacccacttt acagatacag
    19261 atggtcaaga gtttaccatg attgatacgg ctggtatgcg taagtctggt aaggtttatg
    19321 aaaatactga gaaatactct gttatgcgtg ccatgcgtgc tattgaccgt tcagatgtgg
    19381 tcttgatggt catcaatgcg gaagaaggca ttcgtgagta cgacaagcgt atcgcaggat
    19441 ttgcccatga agctggtaaa gggatgatta tcgtggtcaa caagtgggat acgcttgaaa
    19501 aagataacca cactatgaaa aactgggaag aagatatccg tgagcagttc caatacctgc
    19561 cttacgcacc gattatcttt gtatcagctt taaccaagca acgtctccac aaacttcctg
    19621 agatgattaa gcaaatcagc gaaagtcaaa atacacgtat tccatcagct gtcttgaacg
    19681 atgtcatcat ggatgccatt gccatcaacc caacaccgac agacaaagga aaacgtctca
    19741 agattttcta tgcgacccaa gtggcaacca aaccaccaac ctttgtcatc tttgtcaatg
    19801 aagaagaact catgcacttt tcttacctgc gtttcttgga aaatcaaatc cgcaaggcct
    19861 ttgtttttga gggaacaccg attcatctca tcgcaagaaa acgcaaataa aaaagtagaa
    19921 tctggaatga cagttccgga tttttttgat ataataaaat aatagaaaac gctatcaaaa
    19981 gaaggagggt gatgtcggtg atggaacatt tgtttaaatt cttactttta gcaccgtact
    20041 tttattttga taactggatt gagaaggcca acagaaatag taagtttttt ccaatttttt
    20101 attattttta ctggttttac atccccttct attctctttt tagccttgct tggacagttg
    20161 tttcagttct gtttttcaat accgtcttga gaaatgtgac agatatcaag ttatggggca
    20221 tttggtttct ttttattctg ctagctattg gtatgaattg gttaacttat tcctgtttca
    20281 aagaaatgtt tcgcttgaga caggaactag ggaagtctaa gggtggaagg cattgattta
    20341 tatcggtttc tatttgtagg aggtggctta tggtacctct aaaatcattt attacacgct
    20401 attccaaggt ttatattggt aataacattt aatagaacga aactaaagaa aacaatttaa
    20461 aagaggaggt ttgtgatgga atacttgttt aaaatgataa tcttattacc ctgtttttac
    20521 tttttcagct ggattgaaaa ggcaaacaga gatagtaaga ttttcccaat tttttactat
    20581 ttttactgga tttacatcac cctctatgct ctttttagcc ttgcttggac agttttttca
    20641 gttctgtttt tcaatatcgt cttgagaaat ttgacagata tcaagttatg gggtatttgg
    20701 ctattattac tacttatcgc ttttgctagt gactggttag actatatctg tttcaaaaaa
    20761 atgcttgact tgagacggga actagggaag tctaaaggcg gaaggcattg atttatactc
    20821 ttcgaaaatc tcttcaaacc acgtcaacgt cgccttggat tatatatgta actgacttcg
    20881 tcagtcttat ctacaacctc aaagcagtgc tttgagcaac ttgcggctag tttcctagtt
    20941 tgctctttga ttttcattga gtattatatt actttctatt tgtaggaggt ggcttatgaa
    21001 gattcctctc ttaactttgg caaggcataa atttgtttat gtcttgctta ctttgctttt
    21061 tcttgctttg gtttatcgtg atgttttgat gacttatttc ttttttgata ttcatgcgcc
    21121 cgatctagct aaattcgatg gacaagcaat taaaaatgac ttattaaaat cagcattaga
    21181 ttttcgtatt ctccagttca atctaggttt ttatcaatca tttattattc caatcatcat
    21241 tgttttgcta ggttttcaat atattgagct gaaaaataaa gttttacgat tgagtattgg
    21301 aagagaagtg agctatcaag ggttaaaaag aaagttgact ttgcaagttg caagtatccc
    21361 ttgtttgata tatttagtga ctgtgctgat aattgcaatt ataacctatt tctttgggac
    21421 tttttctcct cttgaatgga attctctatt ttctgatggg agtggtttac aaagactcct
    21481 agatggagag ataaaaagct atttgttctt tacttgtgtc ctactaatcg gtattttcat
    21541 caatgcaatc tattttttac aaatagttga ttatgtgggg aatgtgactc gttcggcaat
    21601 cacctatttg atgtttcttt ggcttggttc tatgctgctc tatagtgcct tgccttacta
    21661 tatggttcct atgacgagtt tgatgcaagc tagctatggg gatgtaagtt tgatgaaact
    21721 ctttactcct tatatccttt atgttgtccc ttatatggta cttaaaaaat atgaagataa
    21781 tgtttaagaa ttttaacaat atttggctaa agagaaagat cgttttacta cttcgtatag
    21841 ttctgatgat gattttgata aactatctat tgtcaacagc ggttcaaaag caggatgctg
    21901 ttatcttttt caagagagaa ttgatttcaa ttttttccta taatgactat tctgaagcga
    21961 atttagaaat ccccaaactc ttgttaaacc tttcgctttt catggtagga tggctctctg
    22021 tcattttact tgaaagtgat ttggcagacc attaccatca cttgattcgc tatcaatcaa
    22081 gctccttttt cgattataca aggaaacgat tggttgtcat ttctaaattt tttactcaag
    22141 atttgtttgt ctggtttctt ggtttacttc cgctaggaat tcatttcaaa acagtcgcac
    22201 ttttcttttt acttgctcag ttaatgatgt tgtacttact actgtcttat ctgatagcac
    22261 tgattagtgc gggcgctggt ttttcttttt ttctctattt tttggcattt gtgggacaag
    22321 aatggatgat ggatcatatt gtaacagtgt atttagtact cttaagctta ttagttatgt
    22381 tgattgttag tcgcttggaa gagaaattta agaaaggata aacgatgaga cttgaaatta
    22441 taaatggaca gaaaatttat gggaaaagac ctattttaaa tcagttgaat ttggtgtttc
    22501 aatcaggaaa aatttatgga cttaaaggtg ataatggatc tggcaagacg gttcttttaa
    22561 agatacttgc tggttatatt aagcttgaca aaggaaaagt tcttcaagat ggtaaagttt
    22621 acggggtaaa aaatcattat attcaggatg caggaatttt aattgaaaaa gtcgagtttt
    22681 tatctcattt atccctgaga gaaaatttgg aactgttaag gtatttttca tctaaagtta
    22741 cggaaaaaag aattgcctat tggattcaat actatgattt acaggaattt gaagacgttg
    22801 aataccgtca tttatccttg ggaacaaagc aaaaaatggc cttgattcaa gcctttattt
    22861 cctctccttc tatactcttt ctcgatgaac ctatgaatgc tttggatgag aagagtgtga
    22921 ggttaaccaa acaggtcatt ttatcttacc tgaaaaaaga aaatggtctg gttatcctga
    22981 cgtcgcacat atcggaagat atttcagacc tttgtacaga tgtattagtt gtcgaaaatg
    23041 gacatataca atatgtaaag gatatacaat cctaggagat ggcttatggc acatctaaaa
    23101 tcatttatta cacgctattc caaggtttat attggtttag ttctgctgat ctggctgtct
    23161 ttcttcttta tcccttggga tagagcgatt ctgggggtaa ggattgacat cttcatcatg
    23221 cagaaaatct tgctagcttt tggaattctg tccattctca tggccttgct gtccaagaaa
    23281 gtcagtctct ttgtttttgg actgatttgc tgtctttctc tttggattaa cttatttatc
    23341 acatttgcca ttttgccgat ttttggcaat taaacagtca taaaagtcgg agaggttagc
    23401 ttgaaaacta acctcttttt ccttttcaaa atggggattc ttccttgaaa ataatcagta
    23461 attgtgctaa aattaaagga acattctaaa atattcggaa tttaaagtaa ggaaaaacat
    23521 ggctaatatt ttaaaaacaa ttatcgaaaa tgataaagga gaaatccgtc gtctggaaaa
    23581 gatggctgac aaggttttca aatacgaaga ccaaatggct actttgactg acgaccaact
    23641 aaaagcaaaa acagttgaat ttaaggaacg ttatcaaaat ggagaatcac tggattcatt
    23701 gctttacgaa gcatttgcgg ttgtccgtga aggtgccaaa cgtgtcctag gtctcttccc
    23761 ttataaggtt caggtcatgg gggggattgt tcttcaccat ggtgacgtgc cagagatgcg
    23821 tacaggggaa gggaaaacct tgactgcgac catgccggta tacctcaatg ccctttcagg
    23881 taaaggggtt cacgtagtta cggttaatga atacctgtca gaacgtgacg cgactgagat
    23941 gggtgaattg tactcttggc ttggtttgtc agtagggatt aacttggcta ccaaatctcc
    24001 aatggagaaa aaagaagcct atgagtgtga tattacttac tcaactaact cagaaatcgg
    24061 atttgactac cttcgtgaca acatggtcgt tcgcgccgaa aacatggtac aacgtccgct
    24121 taactatgcc ttggtcgatg aggttgactc tatcttgatt gacgaggctc gtacaccttt
    24181 gattgtatca ggtgccaatg cggttgaaac cagtcagttg tatcacatgg cagaccacta
    24241 tgtaaaatct ttgaacaaag atgactacat catcgatgtg cagtctaaga ctattggttt
    24301 gtctgattca gggattgaca gggctgaaag ctacttcaaa cttgaaaacc tctatgacat
    24361 cgaaaacgtg gctttgactc actttatcga taacgccctt cgtgccaact acatcatgct
    24421 tctcgatatt gactatgtgg tgagcgaaga gcaagaaatc ttgattgtcg accaatttac
    24481 aggtcgtacc atggaaggtc gtcgttattc tgatggattg caccaagcta ttgaagccaa
    24541 agaaggtgtg ccaatccagg atgaaaccaa gacatctgcc tcaatcacgt accaaaacct
    24601 tttccgtatg tacaagaaat tgtctggtat gacgggtaca ggtaagactg aggaagaaga
    24661 attccgtgaa atctacaaca ttcgtgttat tccaatccca acaaaccgtc ctgttcaacg
    24721 tattgaccac tcagaccttc tttatgcaag tatcgaatct aagtttaaag cggttgtcga
    24781 agacgttaag gctcgttacc aaaagggtca acctgtcttg gttggtacag tagcggttga
    24841 aactagtgac tacatttcta agaaattggt tgcagctggt gttcctcacg aagtcttgaa
    24901 tgccaaaaac cactatagag aagcccaaat catcatgaat gctggtcaac gtggtgccgt
    24961 taccatcgca accaacatgg cgggtcgtgg taccgacatc aagcttggtg aaggtgttcg
    25021 tgaacttgga ggactttgtg ttattggtac agaacgtcat gaaagtcgtc gtatcgataa
    25081 ccagcttcgt ggacgttcag gtcgtcaagg agatccaggt gagtcacaat tctacctatc
    25141 tcttgaagat gatttgatga aacgttttgg ttctgaacgc ttgaagggaa tctttgaacg
    25201 tttgaacatg tctgaagagg cgattgagtc tcgcatgttg acgcgtcagg ttgaagcagc
    25261 tcagaaacgt gtcgaaggaa ataactacga tacccgtaaa caagtccttc aatacgatga
    25321 tgtcatgcgt gaacaacgtg agattatcta tgctcaacgt tacgatgtca tcactgcaga
    25381 tcgtgacttg gcacctgaaa ttcaggctat gatcaaacgc acgattgaac gtgtcgttga
    25441 tggtcatgcg cgtgccaaac aagatgaaaa actagaggca attttgaact ttgctaagta
    25501 caacttgctt cctgaagatt ctattacgat ggaagacttg tcaggcttgt ctgataaggc
    25561 catcaaggaa gagcttttcc aacgtgcctt gaaggtttac gatagtcagg tttcaaaact
    25621 acgcgatgaa gaagcagtta aagaattcca aaaagttttg attctacgag tggtggataa
    25681 caagtggaca gatcatatcg atgcccttga tcaattgcgt aacgcggttg gacttcgtgg
    25741 ctatgctcag aacaaccctg ttgttgagta tcaggcagaa ggtttccgta tgtttaatga
    25801 tatgattggt tcgattgagt ttgatgtgac acgcttgatg atgaaagcac aaattcatga
    25861 acaagaaaga ccacaggcag aacgtcatat cagtacaaca gcgactcgca atatcgctgc
    25921 tcaccaagca agtatgccag aagatttgga tttgagccag attggacgca atgaactttg
    25981 cccatgtggt tctggtaaga aatttaaaaa ctgtcacggt aaaagacaat aaaatgagat
    26041 agtttagagg cggatatctt gtgaaaagta aatttttact gggtatccgt ttgctttata
    26101 aggagatgag ttatggtatt tacagcaaaa agctctaaaa taaatataga agaagttcgt
    26161 gccttgtcaa aattagaagg tcaggctttg gagaggaaat cacagcgcga tcaagagcta
    26221 gaagccatta tacgtggaga agatcagcga attctcttgg taatcgggcc atgctcatct
    26281 gacaacgaag aagctgtcct tgaatacgct aagcgtttgg cagtcctaca agaagaagtg
    26341 gcagatcgta tctttatggt tatgcgtgtt tatactgcca aaccccgtac caacggagat
    26401 ggctataagg gcttgattca ccagcctaac gcgacagaag cgcctagtct tatcaatgga
    26461 atcaaagccg ttcgccatct tcactatcgt gtcatcacag aaacagggat gacaactgct
    26521 gatgaaatgc tttatcctga aaaccttccg cttgtagatg atttgatttc ttacatggca
    26581 gttggtgccc gttcagttga agaccagcaa caccgctttg tggcaagtgg ggcaggattt
    26641 tctactggtt ttaaaaatcc aacctctgga aatctcaatg tcatgtttaa tgggatttat
    26701 gctgctcaaa acaaacaaag tttccttttc ttaggaaaag aagtagaaac aactgggaac
    26761 ccgctttcac acgctattct tcgtggtgct cttaatgagt atggaaaaaa tattcccaac
    26821 tactattatg acaatttaat tgataccatt gcccaatatg agaaaatggg cttggaaaat
    26881 ccttttatca tcattgatac caatcatgac aattctggta agcagtatat tgaacagatc
    26941 cgaattgtcc gccagacctt gattaaccgt gcttggaatg aaaaaattaa gcagttcgtt
    27001 cgtggtttta tgattgagtc ttatctggaa gatggtcgac aaaatgagcc agaagtattt
    27061 ggcaagtcta tcacagaccc ttgcctgggt tgggataaca cagaagctct tgtcagagaa
    27121 atttacaaaa cgttaggaga ataagatggc atttattgaa aaaggtcaag aaatcgatat
    27181 ggaagtcatc aaggctgaaa cccaattgtc tgcggaagcc ttgagactca aggaaagccg
    27241 tgacagggaa ttggcagata ttatttcagg ggaagatgac cgtattctct tggtgattgg
    27301 tccttgctct tctgataatg aagaggcggt cttggaatat gctcgccgtt tatccgcctt
    27361 gcaaaagaag gtagcggaca agattttcat ggtcatgcgc gtgtatactg ctaagcctcg
    27421 taccaatgga gacggctata aaggattggt tcaccagcca gatacttcta aggctccaag
    27481 cctgattaac ggtttgcagg ctgtgcgcca gttgcattac cgcgtgatta cagagactgg
    27541 tttgacaacg gcagatgaga tgctttatcc gtcaaatctg atcttggtgg atgacttggt
    27601 cagctaccat gccgttggag ctcgttctgt ggaagaccaa gagcaccgct ttgtggcttc
    27661 tgggattgat gcaccagtag ggatgaaaaa tccaacctca ggaaatttgg gtgttatgtt
    27721 taacgccatc tatgctgctc aaaacaagca aaccttcctt tatcatgggc aggaagttga
    27781 gacatcaggt aatcctttgg cccatgttat cctccgtgga gcagtcaacg agtatggcaa
    27841 ttatatgccg aattactact atgaaaacct actccaagcc attgaacgct atgaaaccat
    27901 gggacttgaa aatcctttta tcctcattga caccaaccat gataactcag gcaagcaata
    27961 tatggagcag attcgaattg ttcgccagac cttgcagaat cgtgattgga atgagaaaat
    28021 taaaaagacg gttcgaggat ttatgattga atcttaccta gcagatggtc gtcaaaacca
    28081 accagagatc tttggttgct ctattactga cccttgccta ggttgggaaa atacagaggc
    28141 cttggtagaa gagatttatg ttaccttgac aaaataagtg aaaaggatgg agttggggaa
    28201 tctcaactcc ttttgatgag aatgatagtt ggacacggaa ttgacatcga agaattggct
    28261 tcgatagaaa gcgcagttac acgacatgaa ggatttgcta agcgtgtact gaccgctcag
    28321 gaaatggagc gcttcaccag tctcaaagga cgcaggcaaa tagaatattt agctggtcgc
    28381 tggtcggcta aggaggcctt ttccaaggct atgggaacgg gcattagcaa gctcggtttt
    28441 caggatttgg aagtattgaa caatgaacgt ggggcgcctt attttagtca ggcaccattt
    28501 tcaggaaaga tttggctgtc tatcagccac accgatcagt ttgtgacagc cagtgtcatt
    28561 ttggaggaaa atcatgaaag ctagtccaca tagaccaacc aaggctctga ttcatctggg
    28621 agctattcga caaaatattc agcaaatggg ggctcatatc cctcaaggaa cgctcaagtt
    28681 ggctgtggtt aaggccaatg cttatggtca tggagctgtt gccgttgcca aggcaattca
    28741 agatgatgtt gatggctttt gcgtttccaa tatcgatgaa gccattgaac tcagacaagc
    28801 tggactcagc aagccaatcc tcattttagg agtttctgaa atcgaagctg ttgctctagc
    28861 taaagaatat gacttcacct tgacagtggc tggactggag tggattcaag cactcttaga
    28921 taaggaagtg gacctaactg gattgacagt ccacctcaag attgattcag ggatgggacg
    28981 gattggtttt agagaggcaa gtgaggttga gcaggctcaa gatttgctcc aacaacacgg
    29041 tgttcgtgtt gaaggaatct ttacccactt tgctactgct gatgaggaat cagatgacta
    29101 ttttaatgcc cagttagaac ggtttaaaac tattttagct agtatgaagg aagttccaga
    29161 gctggttcat gctagcaatt ctgcaacgac tctttggcat gtagagacta ttttcaatgc
    29221 ggttcgtatg ggagatgcca tgtatggtct caatccaagt ggagcggtct tggctttgcc
    29281 ttatgatttg ataccggcct tgaccttgga gtctgctctg gttcatatca agacagttcc
    29341 agctggtgct tgcatgggct atggagcaac ttatcaaacg gatagcgagc aagtcatcgc
    29401 gaccgtgcca atcgggtatg cagatggatg gacaagagac atgcaaaatt tctctgtctt
    29461 ggtagatggc caagcttgcc caattgtcgg cagggtttcg atggaccaaa tcactattcg
    29521 attgcctaag ccttacccac tcggaaccaa ggtaaccttg attggctcca atggggataa
    29581 ggaaatcact gcaactcagg tagcgaccta ccgcgtaacc attaactatg aggtggtttg
    29641 cctcctcagc gaccgtattc cgagagaata ttactagaaa agaaaggagt ggagcatgaa
    29701 tctacatcaa cccttgcatg tcttgcctgg tgtgggacca aagtcagcag aaaaatacgc
    29761 caaactagga attgaaaact tgcaagatct cttgctctac tttcctttcc gttatgaaga
    29821 cttcaaaatc aagcaggtgc tggagctgga agacggtgag aaggcagttc tttctggtca
    29881 ggtagtgact cctgctagtg tccagtatta tggtttcaag cgcaatcgcc tgcgttttag
    29941 tctcaagcag ggagaggtcg tttttgcggt gaatttcttt aaccagccct atctggctga
    30001 taaaatagag ttgggagcaa cccttgctgt ctttggaaaa tgggaccgcg ctaaggctag
    30061 tctgactggg atgaaggttc tggctcaggt agaagatgac ctccagcctg tctatcgtct
    30121 ggctcaggga atcagtcagg ccagtctggt taaggtcatc aagacggctt ttgatcaggg
    30181 actggacctc ttgatagaag aaaatctgcc ccagtcttta ctagacaaat acaaactcat
    30241 gtcccgttgt caggcagtcc gagctatgca ttttccaaag gatttggcag aatacaagca
    30301 ggctcttcgc cgtatcaagt ttgaggaact cttttatttc caaatgcagc tgcagatgct
    30361 caagtctgaa aatagagttc agggaagtgg tctggttctg aattggtctc aggaaaaagt
    30421 gacagcagtt aaagtaagtc ttccttttgc cctgacccaa gctcaggaaa agagtttgca
    30481 ggaaatttta actgatatga agtccgacca ccacatgaat cgtctcctac aaggggatgt
    30541 ggggagtgga aaaacggtag tcgctggctt ggccatgttt gcggcagtga cagcaggtta
    30601 tcaggctgcc ctaatggtac caacagaaat cctcgcagag caacactttg agagtttaca
    30661 gaaccttttt cccaatttga aactggctct cttgacaggt tccttgaaag ctgcagaaaa
    30721 gagagaggtc ttggagacca ttgccaaggg tgaggctgat ttgattatcg gaactcacgc
    30781 tctgatacaa gatggggtgg agtatgctcg tcttggtttg attattatcg atgagcagca
    30841 ccgttttggt gtagggcaaa ggcgtatttt acgggaaaaa ggtgacaatc cagatgtcct
    30901 catgatgacg gcgactccca ttccacggac gcttgccatc acagcctttg gagatatgga
    30961 tgtttccatt atcgaccaga tgccagcagg tcggaagcct attgtgacgc gctggatcaa
    31021 acatgagcaa ctacctcagg tcttgacttg gttagagggg gaaattcaaa aaggttccca
    31081 agcctatgtc atctctcctt tgattgaaga atcagaagct ctagatttga aaaatgccat
    31141 tgccttatca gaggagttga cgactcattt tgcaggcaag gcagaggtgg ctcttctaca
    31201 tggtaggatg aagagtgacg aaaaagacca gatcatgcag gatttcaagg agagaaagac
    31261 ggatattctg gtttcgacga cggttattga ggttggggtc aacgttccca atgcgactgt
    31321 catgattatc atggatgccg atcgcttcgg tctcagtcaa cttcaccagc ttagaggtcg
    31381 tgtcggtcgg ggggacaagc agtcctacgc tgttctcgtt gccaatccca agacggattc
    31441 tgggaaagac cgcatgcgta tcatgacaga aacgaccaat ggatttgtcc ttgcggagga
    31501 agatttgaaa atgcgtggtt ctggtgagat ttttggaacc agacagtcag gacttccaga
    31561 gttccaagtg gctgatatta tcgaagattt tccgatttta gaagaagcaa gaaaggttgc
    31621 tagctacatt agttctatag aagcttggca agaagatcca gagtggcgca tgattgccct
    31681 tcatctggaa aagaaagaac atctggatta agctttctct aaggaaaact taagctagtt
    31741 tttaggtttg gctcttatac taaagtcatc aaaaagaaac gaggactctc atatgacagt
    31801 aacgattaaa gtaaattacc aaaccacttt ccaaaagaag gaagcaaaaa actagtataa
    31861 acagaagaga gagcgaaatg ctctttttcg tttctaaaac tactttcagc ccatcatcct
    31921 aaaagtaaag aatctaaatt cactttctat ttatccttct ttcttgcatt gattacatag
    31981 atatgctaca gttgtggtaa cgattacaaa ataaaaggag catgctatga aaaatccagc
    32041 tttgctagaa gaaattaaga cctatagagg aagggatgag gttccggaag actttgatgc
    32101 tttctgggat ggggaagtga aaaatgtttc cacgcttcca tcctaccact tggaggaaag
    32161 agatttccac attcctcaag tcaagtgcta tgagttaaca tttgaaggaa gcaaggaagg
    32221 aaaggtctat gcacgcattg ttcttccaaa gagtgaggag aaggttccac taatcttcca
    32281 ttttcatggt tatatgggac gtggctggga ctgggccgac atgctgggct tcaccgtagc
    32341 tggttacggt gttgtttcca tggatgtgcg gggccagtca ggttactcac aagacggctt
    32401 gcgttctcct ttaggaaata ccgtgaaggg gcatattatc cgtggtgcta tggaaggtcg
    32461 ggaccacctc ttttataagg atgtttatct ggatatttac cagttggtcg aaattgttgc
    32521 tagtctgtct caggttgatg agaagcgtct ttctagctat ggtgcctcac aaggaggggc
    32581 tctagctcta gttgcagcag cgctcaatcc tcgaattcag aaaacagttg ccatttatcc
    32641 cttcttgtca gacttcagac gggtgattga gattggtaat actagcgagg cttacgacga
    32701 acttttccgt tatttcaagt ttcacgaccc cttccatgaa acagaggagg aaatcatggc
    32761 gacccttgcc tatatcgatg tcaaaaatct tgcccatcgt atccaaggtg aggttaagat
    32821 gattacgggc ttggacgacg atgtttgcta tcccattacc cagtttgcga tttataatcg
    32881 tctgacctgc gataaaacct atcgcatcat gcctgagtat gctcacgaag ccatgaatgt
    32941 atttgtcaat gaccaagtct acaactggct ctgtggaagt gagattcctt ttaaatatct
    33001 aaaataagga gtcgactcta agcacaaaat cttaaaaatt acaaacacgc atagtatcag
    33061 gggattaaaa aaacttgata ctatgcgttt tatcatggaa atatagtaaa atgaaacaag
    33121 aataggacaa aacgatcagg acagtcaaat cgatttctaa caatgtttta gaagcaaatg
    33181 tgtactattc tagtttcaat ctattatatt tatagaattt tttgttgcta gatttgtcaa
    33241 attgcttaaa ataatttttt tcagaaagca aaagccgata cctatcgagt agggtagttc
    33301 ttgctatcgt caggcttgtc tgtaggtgtt aatacttttc aaaaatctct ttaaaccacg
    33361 tcagcttcgc cttgccgtat atatgttact gacttcgtca gttctatctg ccacctcaaa
    33421 acggtgtttt gagctgactt tgtcagtctt atctacaacc tcaaaacagt gttttgagca
    33481 tcatgcggct agcttcttag tttgctcttt gattttcatt gagtataaaa acagatgagt
    33541 ttctgttttc tttttatgga ctataaatgt tcagctgaaa ctactttcaa ggacattatt
    33601 atataaaaga attttttgaa actaaaatct actatattac actatattga aagcgtttta
    33661 aaaatgaggt ataataaatt tactaacgct tataaaaagt gatagaatct atctttatgt
    33721 atatttaaag atagattgct gtaaaaatag tagtagctat gcgaaataac agatagagag
    33781 aagggattga agcttagaaa aggggaataa tatgatattt aaggcattca agacaaaaaa
    33841 gcagagaaaa agacaagttg aactactttt gacagttttt ttcgacagtt ttctgattga
    33901 tttatttctt cacttatttg ggattgtccc ctttaagctg gataagattc tgattgtgag
    33961 cttgattata tttcccatta tttctacaag tatttatgct tatgaaaagc tatttgaaaa
    34021 agtgttcgat aaggattgag caggaagtat ggtataaata gcataagctg atgtccatca
    34081 tttgcttata aagagatatt ttagtttaat tgcagcggtg tcctggtaga taaactagat
    34141 tggcaggagt ctgattggag aaaggagagg ggaaaattgg caccaatttg agatagtttg
    34201 tttagttcat ttttgtcatt taaatgaact gtagtaaaag aaagttaata aaagacaaac
    34261 taagtgcatt ttctggagta aatgtcttat ttcagaaatc gggatataga tatagagagg
    34321 atcagtatga atcggagtgt tcaagaacgt aagtgtcgtt atagcattag gaaactatcg
    34381 gtaggagcgg tttctatgat tgtaggagca gtggtatttg gaacgtctcc tgttttagct
    34441 caagaagggg caagtgagca acctctggca aatgaaactc aactttcggg ggagagctca
    34501 accctaactg atacagaaaa gagccagcct tcttcagaga ctgaactttc tggcaataag
    34561 caagaacaag aaaggaaaga taagcaagaa gaaaaaattc caagagatta ctatgcacga
    34621 gatttggaaa atgtcgaaac agtgatagaa aaagaagatg ttgaaaccaa tgcttcaaat
    34681 ggtcagagag ttgatttatc aagtgaacta gataaactaa agaaacttga aaacgcaaca
    34741 gttcacatgg agtttaagcc agatgccaag gctccagcat tctataatct cttttctgtg
    34801 tcaagtgcta ctaaaaaaga tgagtacttc actatggcag tttacaataa tactgctact
    34861 ctagaggggc gtggttcgga tgggaaacag ttttacaata attacaacga tgcaccctta
    34921 aaagttaaac caggtcagtg gaattctgtg actttcacag ttgaaaaacc gacagcagaa
    34981 ttgccaaatg gtcgagttcg cctgtatgtt aatggtgttt tatctcgaac aagtctgaaa
    35041 tctggcaatt tcattaaaga tatgccagat gtaacgcatg tgcaaatcgg agcaaccaag
    35101 cgtgccaaca atacggtttg ggggtcaaat ctacagattc ggaatctcac tgtgtataat
    35161 cgtgctttaa caccagaaga ggtacaaaaa cgtagtcaac tttttaaacg ctcagattta
    35221 gaaaaaaaac tacctgaagg agcggcttta acagagaaaa cggacatatt cgaaagcggg
    35281 cgtaacggta aaccaaataa agatggaatc aagagttatc gtattccagc acttctcaag
    35341 acagataaag gaactttgat cgcaggtgca gatgaacgcc gtctccattc gagtgactgg
    35401 ggtgatatcg gtatggtcat cagacgtagt gaagataatg gtaaaacttg gggtaaccga
    35461 gtaaccatta ccaacttacg tgacaatcca aaagcttctg acccatcgat cggttcacca
    35521 gtgaatatcg atatggtgtt ggttcaagat cctgaaacca aacgaatctt ttctatctat
    35581 gacatgttcc cagaagggaa gggaatcttt ggaatgtctt cacaaaaaga agaagcctac
    35641 aaaaaaatcg atggaaaaac ctatcaaatc ctctatcgtg aaggagaaaa gggagcttat
    35701 accattcgag aaaatggtac tgtctataca ccagatggta aggcgacaga ctatcgcgtt
    35761 gttgtagatc ctgttaaacc agcctatagc gacaagggtg atctatacaa gggtaaccaa
    35821 ttactaggca atatctactt cacaacaaac aaaacttctc catttagaat tgccaaggat
    35881 agctatctat ggatgtccta cagtgatgac gacgggaaga catggtcagc gcctcaagat
    35941 attactccga tggtcaaagc cgattggatg aaattcttgg gtgtaggtcc tggaacagga
    36001 attgtacttc ggaatgggcc tcacaaggga cggattttga taccggttta tacgactaat
    36061 aatgtatctc acttaaatgg ctcgcaatct tctcgtgtca tctattcaga tgatcatgga
    36121 aaaacttggc atgctggaga agcggtcaac gataaccgtc aggtagacgg tcaaaagatc
    36181 cactcttcta cgatgaacaa tagacgtgcg caaaatacag aatcaacggt ggtacaacta
    36241 aacaatggag atgttaaact ctttatgcgt ggtttgactg gagatcttca ggttgctaca
    36301 agtaaagacg gaggagtgac ttgggagaag gatatcaaac gttatccaca ggttaaagat
    36361 gtctatgttc aaatgtctgc tatccatacg atgcataatg gaaaagaata cattatcctc
    36421 agcaatgcag gtggaccgaa tcgtgaaaat gggatggtcc acttggcacg tgtcgaagaa
    36481 aatggtgagt tgacttggct caaacacaat ccaattcaaa aaggagagtt tgcctataat
    36541 tcgctccaag aattaggaaa tggggagtat ggcatcttgt atgaacatac tgaaaaagga
    36601 caaaatgcct ataccctatc atttagaaaa tttaattggg aatttttgag caaagatctg
    36661 atttctccta ccgaagcgaa agtgaagcga actagagaga tgggcaaagg agagatgggc
    36721 aaaggagtta ttggcttgga gtttgactca gaagtattgg tcaacaaggc tccaaccctt
    36781 caattggcaa atggtaaaac agcgactttc ctaacccagt atgatagcaa aaccttgttg
    36841 tttgcagtag ataaggaaga tatcggacag gaaattattg gtatagctaa aggaagcatc
    36901 gaaagtatgc ataatcttcc tgtaaatcta gcaggtgcca gagttcctgg cggagtaaat
    36961 ggtagcaaag cagcggtgca tgaagttcca gaatttacag ggggagttaa tggtacagag
    37021 ccagctgttc atgaaatcgc agagtataag ggatctgatt cgcttgtaac tcttactaca
    37081 aaagaagatt atacttacaa agctcctctt gctcagcagg cacttcctga aacaggaaac
    37141 aaggagagtg acctcctagc ttcactagga ctaacagctt tcttccttgg tctgtttacg
    37201 ctagggaaaa agagagaaca ataagagaag aattctaaac atttgatttt gtaaaaatag
    37261 aaggagatag caggttttca agcctgctat ctttttttga tgacattcag gctgatacga
    37321 aatcataaga ggtctgaaac tactttcaga gtagtctgtt ctataaaata tagtagattg
    37381 aaataagatg tgaacaactc tatcaggaaa gtcaaattaa tttatagaaa aattttagca
    37441 gtcaaggtgt actgttatag attcaatata ttatgtgact attaaccttc tcttctccta
    37501 aaattgactt tcttgttttc ttatcttgtc cactcgaaac aagtattgta agaatttgat
    37561 tatttttgaa agtactttta atatacttga tatagttaaa aaagatttga aactaaattc
    37621 caaattagaa aaagacttga aatactaaaa aaaaagtata ctctaattga aaacggtaac
    37681 aaaactaatt tagagaatga aatatagagt atttctctct taaaagtttt tggtgaaacg
    37741 agatgtagaa aggagattta gccaaagagt ctattagtgc tagaataata gattagaatt
    37801 attttagaaa aacgaagtga gcagcttata aattcaagtc cccaaataga ttcatactag
    37861 tatcttttgc aaaaaataaa gggcgacttc cttcatgaat atcaatttca tctataagga
    37921 aggtagctaa ttgaactaac ttatttattc tgtttgtcgc tagaaaaatc agacctcctt
    37981 gtgaagattg aggagatact gaatgaaaat caaagaagaa actagcaagc tagtagcaga
    38041 ttgcccaaaa caccgccttg aggttgtaga taagactgac ctatataatc caaggtgaag
    38101 cgactgtggt ttgaagagat tttcaaagag tataggctag agagtagtgt ttttatgtcc
    38161 ttctagtaga aaatgctaga cagaagaatg gggaacttgg ataggaaaaa tagattgaga
    38221 aaggaggtta gaagagatga ttattacaaa aattagccat ttaggaactt atgtgggagt
    38281 aaatccacat tttgcaacat taatagattt tctagaaaaa acaggactag aaaatttaac
    38341 agaaggttcg attgctatcg atggtaatcg attgtttggg aattgcttta cttatctagc
    38401 agatggtcaa gcaggggctt tctttgaaac acaccaaaaa tatttggata ttcatttagt
    38461 tttggaaaac gaagaagcca tggctgttac atcgccggaa aatgtaagcg ttacccaaga
    38521 atatgatgaa gagaaagata ttgaattata cacagggaaa gtggaacagt tggttcattt
    38581 gagagctggt gaatgcctca tcacttttcc agaagattta catcaaccca aggttcgtat
    38641 aaatgatgaa cctgtgaaaa aagttgtctt taaagttgcg atttcttaat gtagaaagag
    38701 aagaacgatg aaaaaaatga gaaagttttt atgtctagct ggaattgcgc tagcggctgt
    38761 tgccttggta gcttgttcag gaaaaaaaga agctacaact agtactgaac caccaacaga
    38821 attatctggt gagattacaa tgtggcactc ctttactcaa ggaccccgtt tagaaagtat
    38881 tcaaaaatca gcagatgctt tcatgcaaaa gcatccaaaa acgaaaatca agattgaaac
    38941 attttcttgg aatgacttct atactaaatg gactacaggt ttagcaaatg gaaatgtgcc
    39001 agatatcagt acagctcttc ctaaccaagt aatggaaatg gtcaactcag atgctttggt
    39061 tccgctaaat gattctatca agcgtattgg acaagataaa tttaacgaaa ctgccttaaa
    39121 tgaagcaaaa atcggagatg attactactc tgttcctctt tattcacatg cacaagtcat
    39181 gtgggttaga acagatttgt taaaagaaca taatattgag gttcctaaaa cttgggatca
    39241 actctatgaa gcttctaaaa aattgaaaga agctggagtt tatggcttgt ctgttccgtt
    39301 tggaacaaat gacttaatgg caacacgttt cttgaacttc tacgtacgta gtggtggagg
    39361 aagcctctta acaaaagatc ttaaagcaga cttgacaagc caacttgctc aagatggtat
    39421 taaatactgg gttaaattgt ataaagaaat ctcacctcaa gattctttga attttaatgt
    39481 ccttcaacaa gctaccttgt tctatcaagg aaaaacagca tttgacttta actctggctt
    39541 ccatatcgga ggaattaatg ccaacagtcc tcaattgatt gattcgattg atgcttatcc
    39601 tattccaaaa atcaaagagt ctgataaaga ccaaggaatt gaaacctcaa acattccaat
    39661 ggttgtttgg aaaaattcaa aacatccaga agttgctaaa gcattcttag aagcacttta
    39721 taatgaagaa gactacgtta aattccttga ttcaactcca gtaggtatgt tgccaactat
    39781 taaggggatt agcgattctg cagcctataa agaaaatgaa actcgtaaga agtttaaaca
    39841 tgctgaagaa gtaattactg aagctgttaa aaaaggtact gctattggtt atgaaaatgg
    39901 accaagtgta caagctggta tgttgactaa ccaacacatt attgaacaaa tgttccaaga
    39961 tatcattaca aatggaacag atcctatgaa agcagcaaaa gaagcagaaa aacaattaaa
    40021 cgatttattt gaggctgttc agtagatgta aaagactaga aaataggtgg gctagtgatc
    40081 tgaaaagctc tagcccaatt ttgtaaaaga agggagaagg agaatggtta aagaacgtaa
    40141 tttaactcgc tggatatttg ttttgccagc tatgattatc gtaggattac tctttgttta
    40201 tccgtttttc tcgagtattt tttatagctt taccaataag catttgatta tgcctaatta
    40261 taaatttgtt ggtttggcta actataaagc tgtgctatca gatcccaact tctttaatgc
    40321 gttctttaat tcaattaagt ggaccgtttt ctcattagtt ggtcaagttt tagtagggtt
    40381 tgtattggct ttagctcttc acagagtacg ccacttcaag aaattatata ggaccttatt
    40441 gattgttcct tgggcatttc ctaccatcgt tattgccttc tcttggcagt ggattctaaa
    40501 cggggtttat ggctacttac ctaatctaat cgtaaaatta ggtttaatgg aacatacacc
    40561 tgcatttttg acagatagta catgggcatt cctatgtttg gtgtttatca acatttggtt
    40621 tggagcacca atgattatgg ttaatgtgct ttcagctttg caaacagtac cagaagaaca
    40681 atttgaggct gctaagatag atggtgcttc aagttggcag gtgttcaagt ttatcgtctt
    40741 tccacatatt aaagtggttg taggacttct agttgttttg agaactgtat ggatctttaa
    40801 taactttgac attatctacc tcattactgg tggtggacca gccaatgcta caacgacgct
    40861 tccaattttt gcttacaacc tgggctgggg aactaaattg ttgggtcgtg cttcagcagt
    40921 tacagtactg ctctttatct tcttggtggc gatttgcttt atctactttg ctatcatcag
    40981 taagtgggaa aaggagggta gaaaataatg aagaagaaat ccagtattta tttagatatt
    41041 ctctcacatg tacttttagt tggtgcgacc atcgttgcag ttttcccatt ggtatggatt
    41101 atcatatctt ctgtcaaagg gaaaggggaa ttaactcagt atccaacacg attttggcct
    41161 gaacagttta cattagatta tttcactcat gttatcaacg atttgcactt cattgataac
    41221 attcgaaaca gtttaatcat tgccttggct acaaccctta ttgcgattat tatttctgct
    41281 atggcagcct atggtattgt tcgattcttt cctaaattgg gagcaatcat gtcgagacta
    41341 ctcgtcatta cctacatttt cccaccaatt ttgttagcaa ttccctattc aattgccatt
    41401 gctaaagttg ggttaacaaa tagtttattt ggcttgatga tggtttatct atcttttagt
    41461 gttccatatg cagtttggct cttagttgga tttttccaaa cagttccaat tggaattgaa
    41521 gaagcggcta gaattgatgg tgcaaataaa tttgttacgt tttataaagt tgtgctaccg
    41581 attgtagcac caggtattgt agcaacagct atttatacat ttatcaatgc ttggaatgaa
    41641 ttcctgtatg ccttgatttt gattaacaat acaggaaaga tgacagtagc agtagccctt
    41701 cgttcactta atggttcaga aatactagac tggggagata tgatggcagc gtctgttatt
    41761 gtagttcttc catcaattat tttcttctct atcatccaaa ataagattgc aagtggatta
    41821 tcagaaggat ctgtgaagta gacgaaagaa ggaaaaaaat gaataaaaga ggtctttatt
    41881 caaaactagg aatttccgtt gtaggcatta gtcttttaat gggagtcccc actttgattc
    41941 atgcgaatga attaaactat ggtcaactgt ccatatctcc tatttttcaa ggaggttcat
    42001 atcaactgaa caataagagt atagatatca gctctttgtt attagataaa ttgtctggag
    42061 agagtcagac agtagtaatg aaatttaaag cagataaacc aaactctctt caagctttgt
    42121 ttggcctatc taatagtaaa gcaggcttta aaaataatta cttttcaatt ttcatgagag
    42181 attctggtga gataggtgta gaaataagag acgcccaaaa gggaataaat tatttatttt
    42241 ccagaccagc ttcattatgg ggaaaacata aaggacaggc agttgaaaat acactagtat
    42301 ttgtatctga ttctaaagat aaaacataca caatgtatgt taatggaata gaagtgttct
    42361 ctgaaacagt tgatacattt ttgccaattt caaatataaa tggtatagat aaggcaacac
    42421 taggagctgt taatcgtgaa ggtaaggaac attacctcgc aaaaggaagt attgatgaaa
    42481 tcagtctatt taacaaagca attagtgatc aggaagtttc aactattccc ttgtcaaatc
    42541 catttcagtt aattttccaa tcaggagatt ctactcaagc taactatttt agaataccga
    42601 cactatatac attaagtagt ggaagagttc tatcaagtat tgatgcacgt tatggtggga
    42661 ctcatgattc taaaagtaag attaatattg ccacttctta tagtgatgat aatgggaaaa
    42721 cgtggagtga gccaattttt gctatgaagt ttaatgacta tgaggagcag ttagtttact
    42781 ggccacgaga taataaatta aagaatagtc aaattagtgg aagtgcttca ttcatagatt
    42841 catccattgt tgaagataaa aaatctggga aaacgatatt actagctgat gttatgcctg
    42901 cgggtattgg aaataataat gcaaataaag ccgactcagg ttttaaagaa ataaatggtc
    42961 attattattt aaaactaaag aagaatggag ataacgattt ccgttataca gttagagaaa
    43021 atggtgtcgt ttataatgaa acaactaata aacctacaaa ttatactata aatgataagt
    43081 atgaagtttt ggagggagga aagtctttaa cagtcgaaca atattcggtt gattttgata
    43141 gtggctcttt aagagaaagg cataatggaa aacaggttcc tatgaatgtt ttctacaaag
    43201 attcgttatt taaagtgact cctactaatt atatagcaat gacaactagt cagaatagag
    43261 gagagagttg ggaacaattt aagttgttgc ctccgttctt aggagaaaaa cataatggaa
    43321 cttacttatg tcccggacaa ggtttagcat taaaatcaag taacagattg atttttgcaa
    43381 catatactag tggagaacta acctatctca tttctgatga tagtggtcaa acatggaaga
    43441 aatcctcagc ttcaattccg tttaaaaatg caacagcaga agcacaaatg gttgaactga
    43501 gagatggtgt gattagaaca ttctttagaa ccactacagg taagatagct tatatgacta
    43561 gtagagattc tggagaaaca tggtcgaaag tttcgtatat tgatggaatc caacaaactt
    43621 catatggcac acaagtatct gcaattaaat actctcaatt aattgatgga aaagaagcag
    43681 tcattttgag tacaccaaat tctagaagtg gccgtaaggg aggtcaatta gttgtcggtt
    43741 tagtcaataa agaagatgat agtattgatt ggaaatacca ctatgatatt gatttgcctt
    43801 cgtatggtta tgcctattct gcgattacag aattgccaaa tcatcacata ggtgtactgt
    43861 ttgaaaaata tgattcgtgg tcgagaaatg aattgcattt aagcaatgta gttcagtata
    43921 tagatttgga aattaatgat ttaacaaaat aaaggagaaa aacatggtta aatacggtgt
    43981 tgttggagca gggtattttg gagctgaatt ggctcgctac atgcaaaaga atgatggagc
    44041 agagattact cttctctatg atccagataa tgcagaggcg attgcagaag aattgggggc
    44101 aaaagtagca agttccttag atgagttggt ttctagcgat gaagtagatt gtgttatcgt
    44161 cgcaactcca aataatcttc ataaggaacc ggttattaag gctgcacagc atggtaaaaa
    44221 tgttttctgt gaaaaaccaa ttgcgctttc ttatcaagat tgtcgcgaga tggtagatgc
    44281 gtgtaaagaa aacaatgtaa cctttatggc aggacatatt atgaatttct ttaatggtgt
    44341 tcatcatgca aaagaactca ttaatcaagg agttatcgga gacgttctat attgtcatac
    44401 agctcgtaat ggttgggaag aacaacaacc gtcagtatca tggaaaaaaa ttcgtgaaaa
    44461 atcaggtggt cacttgtatc accacatcca tgaattggat tgcgttcaat tccttatggg
    44521 gggcatgcct gaaactgtaa ccatgacagg tggaaatgtg gcccatgaag gtgaacattt
    44581 cggtgatgaa gatgatatga tttttgtcaa tatggaattt tctaataagc gttttgcctt
    44641 gttagaatgg ggttcagctt atcgttgggg tgaacattat gtcttaatcc aaggaagcaa
    44701 aggtgccatc cgcttagact tattcaactg taaaggaact cttaagctag atgggcaaga
    44761 aagctatttc ttgattcacg aatcgcaaga agaagatgat gatcggactc gtatctatca
    44821 tagtacagag atggatggag caattgctta tggtaaacca ggtaaacgta ctccattatg
    44881 gctatcatct gtcattgata aagaaatgcg ctatctgcat gagattatgg aaggagctcc
    44941 agtatcagaa gaatttgcaa aacttttgac aggtgaagct gctctagaag caattgctac
    45001 tgcagatgct tgtacccagt ctatgtttga agatcgcaaa gtaaaattgt cagaaattgt
    45061 aaaataaatt ttggtattct cctatttata ggtcgacttg ctcctctgaa agtactttta
    45121 gaggagctgt ttgactttgc tagtttttga aactgaaatc tattatacta caaactattg
    45181 aaagcgtttt aattttaagg tataataatc tcatagaaat aaagaaaagg aggaaagagg
    45241 atgccacaga ttagcaaaga agccttgatt gagcaaatca aagatggaat catcgtttct
    45301 tgtcaggctc ttcctcatga accgctttat acagaagcgg gaggggtgat tcccttgctg
    45361 gtcaaagcgg ctgagcaagg tggagcagtc ggtatccgag caaacagtgt tcgcgatatc
    45421 aaggaaatta aggaggtcac taaacttcca atcattggga ttatcaaacg tgattatcca
    45481 cctcaggaac ccttcatcac ggctactatg aaagaagttg atgaattggc agaactggac
    45541 atcgaggtga ttgctctgga ttgtaccaag cgtgaacgct acgatggttt ggaaattcaa
    45601 gagttcattc gtcaggttaa ggagaaatat cctaatcagc ttttgatggc tgatactagt
    45661 atcttcgaag aagggctagc agctgtagaa gcaggaattg actttgtcgg aacaacctta
    45721 tcaggctaca catcctacag tccaaaagta gacggtccag attttgaatt gattaagaaa
    45781 ctctgtgatg ccggtgtaga tgtcattgca gaaggaaaaa ttcatacacc agaacaagcc
    45841 aaacaaatcc ttgaatatgg agtgcgaggc atcgttgttg gtggcgccat tactagacca
    45901 aaagagatta cagaacgctt cgttgctagt cttaaataag atgtgagggg gagttttatg
    45961 tttaaagttt tacaaaaagt tggaaaagct tttatgttac ctatagctat acttcctgca
    46021 gcaggtctac ttttggggat tggtggtgca ctttcaaacc caaccacgat agcaacttat
    46081 ccaatactag acaatagtat ttttcaatca atattccaag taatgagctc tgcaggagag
    46141 gttgtattca gtaatttgtc actacttctc tgtgtgggat tatgtattgg cttagcgaaa
    46201 cgagataaag gaaccgctgc gttagcagga gtaactggtt acttagttat gactgcaacg
    46261 atcaaagctt tggtaaaact ttttatggca gaaggatctg caattgatac tggagttatt
    46321 ggagcattag ttgtcggaat agttgccgta tatttgcaca accgatataa caatattcaa
    46381 ttaccttccg ctttaggatt ctttggaggt tcacgcttcg ttcctattgt tacatcgttc
    46441 tcttctatct tgattggctt tgtcttcttt gttatttggc cacctttcca acaacttctt
    46501 gtttctacag gtggatatat ttctcaggcg ggtccaattg gaacttttct atatggattt
    46561 ttaatgagac tttctggagc agtaggctta catcatataa tttaccctat gttttggtat
    46621 actgaacttg gtggtgttga aactgttgca ggacaaacag tggttggagc tcaaaaaata
    46681 ttttttgctc aattagccga tttggcccat tctggattat ttacagaagg aacaaggttt
    46741 tttgcaggtc gtttctcaac aatgatgttc ggtttaccgg ctgcctgttt agcgatgtac
    46801 catagtgttc ctaaaaatcg tcgtaaaaaa tacgcgggtt tgttttttgg agttgcttta
    46861 acatctttta ttaccggtat tacagaacca attgaattta tgtttctatt cgtcagtccg
    46921 gttctatatg ttgttcacgc attccttgat ggtgttagct tctttattgc agacgtctta
    46981 aatatttcaa taggaaacac attttcagga ggtgtaatcg atttcacttt atttggaatt
    47041 ttgcagggga acgctaagac gaattgggtt cttcagattc catttggact tatttggagt
    47101 gttttgtatt atattatttt tagatggttc attactcaat tcaacgttct aacgccaggg
    47161 cgaggagaag aagtagattc taaagaaatt tctgaatccg cagattcaac ttcaaatact
    47221 gcagattatt taaaacagga tagcctacaa attatcagag ccttgggtgg atcaaataat
    47281 atagaagatg tagatgcttg tgtgacacgt ttacgtgtag ctgtaaaaga agttaatcaa
    47341 gttgataaag cacttttaaa acaaattggt gcagttgatg tcttagaagt gaagggtggc
    47401 attcaagcaa tctatggagc aaaagcaatc ttatataaaa atagtattaa tgaaatttta
    47461 ggtgtagatg attaagtact tactgactta ataaaaaaca gaggagagtg atggatgagt
    47521 aggatgaaat gaaatcgcat acaagaaata aagaactcat tatccaagtt ggatacgctt
    47581 attacatagg agaatacaaa tgaaatttag aaaattagct tgtacagtac ttgcgggtgc
    47641 tgcggttctt ggtcttgctg cttgtggcaa ttctggcgga agtaaagatg ctgccaaatc
    47701 aggtggtgac ggtgccaaaa cagaaatcac ttggtgggca ttcccagtat ttacccaaga
    47761 aaaaactggt gacggtgttg gaacttatga aaaatcaatc atcgaagcgt ttgaaaaagc
    47821 aaacccagat ataaaagtga aattggaaac catcgacttc aagtcaggtc ctgaaaaaat
    47881 cacaacagcc atcgaagcag gaacagctcc agacgtactc tttgatgcac caggacgtat
    47941 catccaatac ggtaaaaacg gtaaattggc tgagttgaat gacctcttca cagatgaatt
    48001 tgttaaagat gtcaacaatg aaaacatcgt acaagcaagt aaagctggag acaaggctta
    48061 tatgtatccg attagttctg ccccattcta catggcaatg aacaagaaaa tgttagaaga
    48121 tgctggagta gcaaaccttg taaaagaagg ttggacaact gatgattttg aaaaagtatt
    48181 gaaagcactt aaagacaagg gttacacacc aggttcattg ttcagttctg gtcaaggggg
    48241 agaccaagga acacgtgcct ttatctctaa cctttatagc ggttctgtaa cagatgaaaa
    48301 agttagcaaa tatacaactg atgatcctaa attcgtcaaa ggtcttgaaa aagcaactag
    48361 ctggattaaa gacaatttga tcaataatgg ttcacaattt gacggtgggg cagatatcca
    48421 aaactttgcc aacggtcaaa catcttacac aatcctttgg gcaccagctc aaaatggtat
    48481 ccaagctaaa cttttagaag caagtaaggt agaagtggta gaagtaccat tcccatcaga
    48541 cgaaggtaag ccagctcttg agtaccttgt aaacgggttt gcagtattca acaataaaga
    48601 cgacaagaaa gtcgctgcat ctaagaaatt catccagttt atcgcagatg acaaggagtg
    48661 gggacctaaa gacgtagttc gtacaggtgc tttcccagtc cgtacttcat ttggaaaact
    48721 ttatgaagac aaacgcatgg aaacaatcag cggctggact caatactact caccatacta
    48781 caacactatt gatggatttg ctgaaatgag aacactttgg ttcccaatgt tgcaatctgt
    48841 atcaaatggt gacgaaaaac cagcagatgc tttgaaagcc ttcactgaaa aagcgaacga
    48901 aacaatcaaa aaagctatga aacaatagtc cttagttatt ctataaaaag tagtttttta
    48961 aagaacctaa gagtgtatac ccccttttcc ctctacacag atagtgtaag aaaagggggc
    49021 ttttgtttaa aatgtaagaa actgtcacga aattaaaatg aagttcttac ataagcgaat
    49081 cataaaaaat ttcattttga ttttaaaaca gttcaagaaa gtcaaaaaat tattctattt
    49141 gaaagagagg tgccgactgt gaaagtcaat aaaatccgta tgcgggaaac agtgatttcc
    49201 tacgctttcc tagcaccagt attattcttc tttgtcatct ttgtgttggc tccgatggtg
    49261 atgggcttca ttacaagttt ctttaactac tcaatgacta aatttgagtt tgtaggcttg
    49321 gataactata tccgtatgtt taaagatcct gtctttacaa aatctctgat taacacagtt
    49381 attttggtta ttggatctgt accagttgtt gttctattct cactctttgt agcatctcag
    49441 acctatcatc aaaatgtcat tgccagatcc ttctaccgtt tcgtcttctt ccttcctgtt
    49501 gtaacgggta gtgttgccgt gacagttgtt tggaaatgga tttatgaccc actatcaggg
    49561 attctaaact ttgtccttaa gtccagccac atcatcagcc aaaacatttc ttggttggga
    49621 gataaaaact gggcattgat ggcgattatg attattctct tgaccacttc agttggtcag
    49681 cccatcatcc tttatatcgc tgccatgggg aatattgaca attcactggt tgaagcggcg
    49741 cgtgttgatg gtgcaactga gtttcaagtt ttttggaaga ttaaatggcc aagccttctt
    49801 ccaacaactc tttatattgc aatcatcaca acaattaact cattccagtg tttcgccttg
    49861 attcagcttt tgacatctgg tggtccaaac tactcaacaa gtaccttgat gtactacctt
    49921 tacgaaaaag ccttccaatt gacagaatac ggctatgcca acacaattgg tgtcttcttg
    49981 gcagtcatga ttgctatcgt aagctttgtt caatttaaag tacttggaaa cgacgtagaa
    50041 tactaaagaa aggagacagc tatgcaatct acagaaaaaa aaccattaac agcctttact
    50101 gttatttcaa caatcatttt gctcttgttg actgtgctgt tcatctttcc attctactgg
    50161 attttgacag gggcattcaa atcacaacct gatacaattg ttattcctcc tcagtggttc
    50221 cctaaaatgc caaccatgga aaacttccaa caactcatgg tgcagaaccc tgccttgcaa
    50281 tggatgtgga actcagtatt tatctcattg gtaaccatgt tcttagtttg tgcaacctca
    50341 tctctagcag gttatgtatt ggctaaaaaa cgtttctatg gtcaacgcat tctatttgct
    50401 atctttatcg ctgctatggc gcttccaaaa caagttgtcc ttgtaccatt ggtacgtatc
    50461 gtcaacttca tgggaatcca tgatactctc tgggcagtta tcttgccttt gattggatgg
    50521 ccattcggtg tcttcctcat gaaacagttc agtgaaaata tccctacaga gttgcttgaa
    50581 tcagctaaaa tcgacggttg tggtgagatt cgtaccttct ggagtgtagc cttcccgatt
    50641 gtgaaaccag ggtttgcagc ccttgcaatc tttaccttca tcaatacttg gaatgactac
    50701 ttcatgcaat tggtaatgtt gacttcacgt aacaatttga ccatctcact tggggttgcg
    50761 accatgcagg ctgaaatggc aaccaactat ggtttgatta tggcaggagc tgcccttgct
    50821 gctgttccaa tcgtcacagt cttcctagtc ttccaaaaat ccttcacaca gggtattact
    50881 atgggagcgg tcaaaggata atactctgcg aaaatctctt caaactacgt cagcgtcacc
    50941 ttgccatact taagtattgc ctgcggttag cttcctagtt tgttcttcaa ttttcattga
    51001 gtataggaaa atcaatctat caagatacag aagtatattt tatagattta gagaatatag
    51061 aggttataag tgtctacaaa atggagggta tgcagttact ttatgaagtt ttgtcagaca
    51121 cttataaact taagaatggt tttagttaac tatcagaaac gaaggaaaga gtatgatttt
    51181 tgacgatttg aaaaacatca ccttttacaa agggattcat cctaatttag acaaggctat
    51241 cgactatctc taccaacatc gtaaggattc tttcgaatta ggaaagtatg agattgatgg
    51301 agataaagtc tttctagttg ttcaggaaaa tgtcctcaat caagctgaaa atgatcaatt
    51361 tgagtatcat aagaactatg cagatttgca tttgctggta gaaggacatg aatattcgag
    51421 ctacggttca cgtatcaaag acgaggcagt agcattcgac gaagcgagtg acattggctt
    51481 tgttcattgt catgaacact acccactctt gttgggttat cacaattttg cgattttctt
    51541 cccaggtgag ccacatcagc caaatggtta tgcaggcatg gaagaaaagg ttcgaaaata
    51601 tctctttaaa attttgattg attaaaaata ggatgaattg tttttttgta aagctttgac
    51661 aatactctac catgaaattg atctttgtga ggtagagaaa tgagaataaa atatttaaaa
    51721 attggtatct tctaagcatg ctgcaagagc tagtttctta gatggacagg gaattacagt
    51781 tgatgagacg tcctggataa ttaatggcat tgtgaatgca ttgattggta gatacataaa
    51841 attaggtact tatgcagcta agtatggtat tagtatagca cgctcgatct taagtagggc
    51901 agctgcaact gcagcagcaa gagtaggatt actgaccaag atttctggat ggattttacg
    51961 agtagctgtg aatgtagctg atgtatatgg taattttgcc aacaatattg ctgcagcttg
    52021 ggatgcatat gataaaattc ctaacaatgg tcgtataaac ttttaaaatg cgagaatgaa
    52081 agcactttgt atttttttat tgaatatgtt agcttggaca gtgcttgcaa tgataattcg
    52141 tggagggcta gatggatttg atgagcatac ttggagtact attttaattg cttcgctgtt
    52201 cggggtatat gattataagc ccatagataa aaatagaaaa aagtccaaaa gaaaaaagaa
    52261 aaaatagatt tgttcatggt agggacttat gaaagcttta ctgacaaaaa agaaaacagt
    52321 ttacaaagaa aaatgatgga ggagcaaaca tggcacaaaa aggagtaagc cttatcaagg
    52381 cagcatttga tacagataac tttctcatgc gttttagtga gaaagtcttg gacatcgtga
    52441 cagccaatct tctttttgtc atctcttgtt tgcccatcgt gacgattgga gtggctaaaa
    52501 tcagcctcta cgagaccatg ttcgaagtta agaagagcag acgggtgcct gtttttaaaa
    52561 tctatctaag atctttcaag caaaatctga aactaggtct tcagctgggt ttaatggagt
    52621 taggaattgt gtttcttacc ctttcagacc tctatctttt ctggggtcaa acagctctgc
    52681 ccttccaatt gctgaaagcc atttgtttag gtattctgat ttttcttact atcgtgatgc
    52741 tggctagtta ccctatcgcg gcacgttatg acctatcttg gaaagaaatt cttcaaaaag
    52801 gattgatgtt ggctagtttt aactttcctt ggttcttcct catgttagcc attcttgtcc
    52861 tcattgtgat ggttctttat ctgtccgcct tcagtctact cttaggtggc tcagtcttcc
    52921 tactttttgg gtttggacta ttggtcttta tccagactgg attgatggag aaaattttcg
    52981 caaaatacca ataggagcct tatttctgaa actactttca aaggttccaa acgctattct
    53041 ataagcgaga aactaaaatc taagtgtcta taagatattg aaagcctttt cggtaaagtg
    53101 tatactaaag aagtaaagaa aaatctgctt tagcagaaaa taaaaataat aggagaaaat
    53161 ctatgtcaga tttgaaaaaa tacgaaggtg tcattccagc cttctacgca tgttatgatg
    53221 atcaaggaga agtaagccca gaacgtacgc gtgccttggt tcaatacttc attgataaag
    53281 gtgttcaagg tctttatgtc aatggttctt ctggtgaatg tatctaccaa agcgttgaag
    53341 atcgcaagtt gattttggaa gaagttatgg cggttgccaa aggtaaattg accattattg
    53401 cccatgttgc ctgcaacaat accaaggata gtatggaact tgctcgccat gctgaaagct
    53461 tgggagtaga tgctattgca acgattccac caatttattt ccgcttgcca gaatactcag
    53521 ttgccaaata ctggaacgat atcagttctg cagctccaaa tacagactac gtgatttata
    53581 acattcctca attggcaggt gttgctttga ctccaagcct ttacactgaa atgttgaaaa
    53641 atcctcgtgt tatcggtgtg aagaactctt ctatgccagt tcaagatatt caaacctttg
    53701 tgagccttgg tggggaagac catatcgtct ttaacggtcc agatgaacag ttcctaggtg
    53761 gtcgcctcat gggtgccaaa gctggtatcg gtggtactta tggtgctatg ccagaactct
    53821 tcttgaaact caatcagttg attacggata aggacctaga aacagcgcgt gaattgcagt
    53881 atgctatcaa cgcaatcatt ggtaaactca cttctgctca tggaaatatg tacggtgtca
    53941 tcaaagaagt cttgaaaatc aatgaaggct tgaatattgg atctgttcgt tcaccattga
    54001 caccagtgac tgaagaagat cgtccagttg tagaagcggc tgctgccttg attcgtgaaa
    54061 ccaaggagcg cttcctctaa tctaaaagga ggtatttatg acatattacg ttgcaattga
    54121 tatcggtgga accaacatca agtatggttt ggttgatcaa gaggggcaac ttcttgaatc
    54181 gcatgaaatg ccaactgagg cgcataaggg tggacctcat atcttacaaa agaccaaaga
    54241 tatcgtagct agttatttag aaaaaggccc agtagcaggt gttgccatat cttctgctgg
    54301 gatggtggat ccggataagg gtgagatttt ctatgctggg ccgcaaatcc ctaactacgc
    54361 aggcacccag ttcaaaaagg aaatcgaaga aagctttact attccttgtg agattgaaaa
    54421 tgatgtcaac tgtgcaggtc ttgctgaggc agtatctggt tcaggcaagg gagcaagtgt
    54481 gacactttgc ttgaccattg gaacaggtat cggtggttgc ttgattatgg ataggaaagt
    54541 cttccatggt tttagcaatt cagcctgtga agtcgggcat atgcatatgc aggatggagc
    54601 ttttcaagac ttggcttcta caacagcttt agtgaaatat gtagctgaag cccatggaga
    54661 agatgttgat cagtggaatg gccgtagaat tttcaaagaa gccactgaag gaaacaaaat
    54721 ctgcatggaa ggtattgacc gtatggttga ctatctagga aaaggtctgg caaatatttg
    54781 ctacgttgcc aatccagaag tggtcattct tggtggtggt atcatggggc aagaggctat
    54841 cctcaaacct aagatccgca cagccttgaa agaggctttg gtaccaagtt tagcagaaaa
    54901 aacacgatta gaatttgccc atcaccaaaa tacagcaggg atgttgggtg catattatca
    54961 ttttaagaca aaacaatcct agtttggctc agccaaacta ggattttctt acacgttttt
    55021 gtctacgata gccgttgagt tttttatttt cccagtagct attaaagatt ttttccttgc
    55081 tttcgcgatt gatttccaaa aagtaggcat aaatcaaatc gataaagaag agcataggaa
    55141 gttgagcgga tattcgttgg atgtaggagg gttggctgtg ggtggctaca agaacagtct
    55201 ctgtataggt ctggctattt ttattgggaa cacttgtaaa gagtacagtc tttgccccca
    55261 tctccttggc atctaataga ctatctaaaa tagaaggagt tgagcctgaa agtgagaagc
    55321 ccagtactag acaattttca tccatgatgc tggttgtcca ggcaaagccg tcttggtctg
    55381 tcaaagcttc gcagaccaca cctaatcgca taaaacgtaa tttcatttca cgggcgacga
    55441 ggccagaact ccctgttcca aagaagtaga tacgctcagc atcttcgatt agctgggcaa
    55501 ttcgctctag ttggatttcg tcaatcaagt cttgtgtttg ttccctcata ttgctataac
    55561 ttctgaggac tcgtttggtc agtggactgt gcttggagac ttggttggct tgattttctg
    55621 cctgatgttg gtattggaaa ataaattctc ggtagccagt aaagccacac tttttagcaa
    55681 agcgggtcaa agcagcttga gaaatatgta atttttgggt gacttgttga gaagataaat
    55741 catctgtaat cgtttcagct tgcaaaaaat agcgagcgat ttcttgctct aggtctgtca
    55801 tttcttcaaa atgtgaatca atgatagttg cgatatctgg tttgtccata gggaaagctc
    55861 ctttacatga gtcatactgg aagactagat cagggaatag tcacacttca ttataacaca
    55921 taatataagg atagataaat aaaaacgcat ctctgtttta aaaacgaaaa aatcgaaaaa
    55981 gcttctctct tttccataat tttctactca aattgtggta caattaagag taagatttta
    56041 agttagaaat gagactgatt tgtatgagaa aatttaacag ccattcgatt ccgattcggc
    56101 ttaatttatt gttttcaatc gtcattttac tctttatgac cattattggt cgtttgttgt
    56161 atatgcaggt tttgaacaag gatttttacg aaaaaaagct agcctcagct agtcagacca
    56221 agattacaag cagttcagcc cgtggggaaa tttatgatgc tagtggaaaa cctttggtag
    56281 aaaatacgtt aaagcaggtt gtttccttta cgcgtagcaa taaaatgacg gctacagact
    56341 taaaagaaac agctaaaaag ttactgactt atgtgagcat cagttctcca aatttgacag
    56401 aacgccagct ggcggattac tatttggctg atcctgaaat ctataaaaaa atagtggaag
    56461 ctctcccaag tgagaaacgc ttggattcag atggcaatcg tctatccgaa tcagaactgt
    56521 ataacaatgc ggtcgatagt gtacaaacga gtcaactaaa ctatacagag gatgaaaaga
    56581 aagaaatcta tctttttagt cagttaaatg ctgttggaaa ctttgcgaca ggaaccattg
    56641 cgacagatcc tctaaatgat tctcaggtgg ctgttattgc ctctatttca aaggagatgc
    56701 ctggcattag tatttctact tcttgggata gaaaggtttt ggaaacttcc ctttcttcta
    56761 tagttgggag tgtatccagt gaaaaagctg gtctcccagc ggaagaagca gaagcctatc
    56821 ttaaaaaagg ctattctcta aatgatcgtg taggaacctc ctatttggaa aagcaatatg
    56881 aagagacctt acaaggaaaa cgctcggtaa aagaaatcca tctggataaa tatggcaata
    56941 tggaaagcgt ggatacaatt gaggaaggta gtaagggaaa caatatcaaa ctgaccattg
    57001 atttggcctt ccaagatagc gtggatgctt tactgaaaag ttatttcaat tccgagctag
    57061 aaaatggtgg agccaagtat tctgaaggtg tctatgcagt cgcccttaac ccaaaaacag
    57121 gtgcggtttt gtctatgtca gggattaaac atgacttgaa aacgggagag ttgacgcctg
    57181 attccttggg aacggtaacc aatgtctttg ttccaggttc ggttgtcaag gcggcgacca
    57241 tcagctcagg ttgggaaaat ggagtcttgt caggaaacca gaccttgaca gaccagtcca
    57301 ttgtcttcca aggttcagct cccatcaatt cttggtatac tcaggcttac ggttcattcc
    57361 ctatcacagc ggtccaagct ctggagtatt catctaatac ctatatggtc caaacagcct
    57421 taggtcttat ggggcaaacc tatcaaccca atatgtttgt cggcaccagc aatctagagt
    57481 ctgctatgga gaaactgcgt tcaacctttg gcgaatatgg cttgggtact gcgacaggaa
    57541 ttgacctacc agatgaatct actggatttg ttcccaaaga gtatagcttt gctaattaca
    57601 ttactaatgc ctttgggcag tttgataact atacgccgat gcagttggct cagtatgtag
    57661 caactattgc aaataatggt gttcgtgtgg ctcctcgtat tgttgaaggc atttatggta
    57721 ataatgataa gggaggactg ggtgacttga ttcagcaact gcaaccgaca gagatgaata
    57781 aggtcaatat atccgactcc gatatgagca tcttgcacca aggtttttat caggttgccc
    57841 atggtactag tggattgaca actggacgtg ccttttcaaa tggtgccttg gtatccatta
    57901 gcggaaaaac aggtacagcc gaaagctatg tggcagatgg tcagcaagca accaatacca
    57961 atgcggtggc ctatgcccca tctgataatc cccaaatcgc tgtcgcagtg gtctttcctc
    58021 ataataccaa tctaacaaat ggtgtaggac cttccattgc gcgtgacatt atcaatctgt
    58081 atcaaaaata ccatccaatg aactagaaag gaaattatgc tttatccaac acctattgcc
    58141 aagttgattg acagttattc taagttacca ggtatcggga ttaagacggc tacgcgtctg
    58201 gccttttata cgattgggat gtctgctgat gatgtcaatg aatttgcaaa aaatctcctt
    58261 tctgctaaga gagaattgac atattgttct atttgtggac gtttgacaga cgacgatcct
    58321 tgttctatct gtaccgatcc gactcgtgac cagacaacaa ttttagttct tgaggatagt
    58381 agagatgtgg caaccatgga aaatatccaa gaataccatg gcctctatca tgtcctgcat
    58441 ggcctcattt ctcctatgaa tggtatcagt ccggacgata tcaatctcaa gagccttatg
    58501 actcgtctta tggatagtga agtttcagaa gtgattgtgg cgactaatgc tacagcggat
    58561 ggtgaagcga cttccatgta tctttcacgt ttgctcaagc cggctggtat caaggttacg
    58621 cgtctagcac gaggtctcgc tgtgggagcg gacattgagt atgcggacga agtgacactc
    58681 ttacgagcca ttgaaaatcg gacagagttg taagtgtagg caaatttacg aactccattc
    58741 atttataaaa aatcaaagag gctgaaaatc gttcctatcg gcctcttttt gtatagtgtg
    58801 atgagtagga tcgggttcaa gttttaaaaa accaagcaaa tatgatatac taaagagcga
    58861 gtattctagt agaattagga caaataatat gaaacaaacg attattcttt tatatggtgg
    58921 acggagtgcg gaacgcgaag tctctgtcct ttcagctgag agtgtcatgc gtgcggtcaa
    58981 ttacgaccgt ttcacagtca agactttctt tatcagtcag tcaggtgact ttatcaaaac
    59041 acaggaattt agtcatgctc cggggcaaga agaccgtctc atgaccaatg aaaccattga
    59101 ttgggataag aaagttgcac caagtgctat ctacgaagaa ggtgcagtgg tctttccagt
    59161 ccttcacggg ccaatgggag aagatggctc tgttcaagga ttcttggaag ttttgaaaat
    59221 gccttacgtt ggttgcaaca ttttgtcatc aagtcttgcc atggataaaa tcacgactaa
    59281 gcgtgttctg gaatctgctg gtattgccca agttccttat gtggctatcg ttgaaggcga
    59341 tgatgtgact gctaaaatcg ctgaagtgga agaaaaattg gcttatccag tcttcactaa
    59401 gccgtcaaac atggggtcta gtgtcggtat ttctaagtct gaaaaccaag aagaactccg
    59461 tcaagcctta aaacttgcct tccgatatga cagccgtgtc ttggttgagc aaggagtgaa
    59521 tgcccgtgaa attgaggttg gcctcttggg taactacgat gtcaagagca cgctacctgg
    59581 agaagttgtc aaggacgttg ccttttatga ctacgatgcc aagtatattg ataacaagat
    59641 tactatggat attcctgcca aaatcagtga tgatgtggta gctgtcatgc gtcaaaatgc
    59701 agaaacagcc ttccgtgcca ttggtggcct tggtctatct cgttgcgatt tcttctatac
    59761 agataaggga gagatttttc tcaacgagct caataccatg ccaggtttca cccagtggtc
    59821 tatgtaccca ctactttggg acaatatggg gatcagctac ccagaactaa tcgagcgttt
    59881 ggttgacctt gccaaggaaa gttttgacaa gcgcgaagcg catttgatat aaaatgaaag
    59941 agagggtaga agccagaacc atcactgcaa ggtgactaga gttctcggac ttcagccctt
    60001 tttaaaggag tagaaatgaa attaacaatc catgaagttg cccaagttgt tggagccaaa
    60061 aatgatatca gtatctttga ggacacccag ttagaaaaag ctgagtttga tagtcgtttg
    60121 attggaactg gagatttatt tgtgccccta aaaggtgtgc gtgacggtca tgactttatc
    60181 gaaagagctt ttgaaaatgg tgcggcagca accctgtctg agaaagaggt tgcaaatcat
    60241 ccctacattc tagtagatga tgttttgact gcctttcaac aactagctgc ctactatctt
    60301 gaaaaaacag ctgttgatgt ctttgcggtt acgggttcaa atggcaagac aacgactaag
    60361 gatatgttgg cgcatttact gtcaacaaga tacaagacct acaaaacaca aggcaattac
    60421 aataatgaga ttggccttcc ttacacagtt ctccacatgc ctgaaggaac agaaaagttg
    60481 gtcttggaga tggggcagga ccacttggga gatattcatc tcttgtctga attggctcgt
    60541 ccaaaaacag ccatcgtgac cttggttgga gaagctcatt tggccttttt caaagatcgt
    60601 tctgagattg ctaaaggaaa aatgcaaatt gcagacggaa tggcttcagg ttccttgctt
    60661 ttagcgccgg ctgaccctat cgtagaggac tatttgccaa ctgataaaaa ggtggttcgt
    60721 tttggtcaag gagcagagct ggaaattact gacttggttg agcgcaaaga tagtctgacc
    60781 ttcaaggcca atttcttaga gcaagtcctt gatttgccag taactggcaa gtacaatgcg
    60841 acaaatgcta tgattgcatc ctatgttgcc ttgcaagaag gagtttcaga ggagcaaatt
    60901 cgtttggcct tccaacatct tgaattgacg cgtaaccgta ccgagtggaa gaaagcagcc
    60961 aatggagcag acatcttgtc agatgtttac aatgccaatc caactgctat gaagctgatt
    61021 ttagagacct tctctgccat tccagccaat gaaggtggca agaaaattgc agtgttggcg
    61081 gatatgaagg aacttggtgt ccagtctgtt caacttcata atcagatgat tttgagcctt
    61141 tcgccagatg tgcttgatac tgttattttc tacggagaag atattgctga attagcccaa
    61201 cttgctagtc aaatgttccc aatcggacac gtttattact tcaagaaaac agaagaccaa
    61261 gaccaatttg aagacctagt taagcaggtc aaagaaagcc ttggggctca tgaccaaatc
    61321 ctgctcaaag gctctaactc tatgaatcta gccaagttgg tagaaagttt agaaaatgaa
    61381 gacaagtgat tttgtcaagt atttgcaaag aatgattgcc attacagata ctggattaac
    61441 ctttacaaaa gatccgtttg accgtgagcg ctacgaagac ttgcgagacc ttctatctga
    61501 aatgttgaat caagcatcag accttgattc cgaagaagtg gcagaagtct tgaagccaac
    61561 ttctgcttat gcgactccct taatggacgt ccgtgcttgg attgttgagg acgaaaagat
    61621 ttgtctggtt aggggacaag gagaggatag ttgggctttg ccaggtggct ttggtgaagt
    61681 cggctattct ccaactgaaa atattctcaa ggaaattgaa gaagaaaccg gttttaaagc
    61741 caaagttgaa agactgctag ctgtttttga taccaatcgt ttccaactac agagcaaaca
    61801 atatgcaaag tttgtttttg aatgtaagct tctcgatgga caattccaag aaaatcaaga
    61861 aatcgctgac cttcaatttt ttgcaattga cccactgccg aatttatctg aaaaacgcat
    61921 taccaaggag caaatcgaga ttctttggca ggtttatcaa ggtcagaggg agcaatatct
    61981 tgactaagaa gatgattatc gtatttctaa attcattttt cttaagtagc atggtataat
    62041 aaaatgcagg aaaattttga atcatgagga agactagatg aatttatggg atattttctt
    62101 tacgactcag gcaaccgagc cgcccaaatt tgaccttttt tggtatgtta gcctatttac
    62161 gctcttagcc ttaacctttt atacagccca tcgctatcgt gaaaagaagg tttaccaacg
    62221 atttttccaa atcttgcaga ctgttcagtt aatccttctt tatggttggt actgggtcaa
    62281 tcatatgcca ctgtcagaaa gcctaccctt ttaccattgc cgtatggcta tgtttgtggt
    62341 actcttgctt cctggtcagt ccaaatataa acaatacttt gcattattgg gaacatttgg
    62401 gacattagca gcctttgttt atccagtgcc agatgcttac ccttttccac atatcaccat
    62461 tctatccttt atctttggtc atttagcact cttggggaac tctctagttt atctattgag
    62521 acagtataat gcgcgattgc tggatgtgaa gggaattttt ctcatgacct ttgccctaaa
    62581 tgccttgatt tttgtggtca atttggtgac aggtggcgat tacggatttt tgacaaaacc
    62641 gccattggtt ggggatcacg gtctagtagc taattattta cttgtttcaa ttgtgctggt
    62701 agctactatc agtttgacta agaaaatctt agaattcttt ttagctcaag aagcagaaaa
    62761 aatgattgca aaggaagctt aacacagagc tttctttttt gctcttagag agtttttaca
    62821 agcagcttat aaaataagaa tttctgaata gacaaactca aaaaatggct gggaaattta
    62881 ggaaaaaagc aagcacgatt aaattttttg tgttataata ttttgtgaat agctatgcct
    62941 atgtttagct atggaataat acgaagtgcg aaacttggaa gatagagagg aagcgatgta
    63001 atggctagag aaggcttttt tacaggtcta gatattggaa caagctctgt caaggtgctt
    63061 gtggccgagc agagaaatgg tgaattaaat gtaattggcg tgagtaatgc caaaagtaaa
    63121 ggtgtaaagg atggaattat tgttgatatt gatgcagcag caactgctat caagtcagcc
    63181 atttcccaag cggaagaaaa ggcaggcatt tcgattaaat cagtaaatgt cggcttgcct
    63241 ggtaatcttt tgcaggtaga accaactcag gggatgattc cagtaacatc tgatactaag
    63301 gaaattacgg atcaagatgt tgaaaatgtt gtcaaatcag ctttgacaaa gagtatgaca
    63361 cctgaccgtg aagtcattac ctttattcct gaagaattta ttgtggatgg tttccaaggg
    63421 attcgtgacc cacgtggcat gatgggggtt cgccttgaaa tgcgtggttt gctttataca
    63481 ggacctcgta ctatcttgca caatttgcgt aagacggttg agcgtgcagg tgttcaggtt
    63541 gaaaatatta tcatttcacc actagcaatg gttcagtctg ttttgaatga aggggaacgt
    63601 gaatttggtg ctacagtgat tgatatgggg gcaggtcaaa cgactgtcgc tacaatccgt
    63661 aatcaagaac tccagttcac acatattctc caagaaggtg gagattatgt aactaaagat
    63721 atctccaagg ttttgaaaac ctctcgcaaa ttagcggaag gcttgaaact gaattacggg
    63781 gaagcctatc cgcctcttgc aagcaaagaa accttccaag tagaggttat tggagaagtg
    63841 gaagcagtcg aagtgacgga agcctacttg tcagaaatta tttctgcacg aatcaagcac
    63901 atccttgaac aaatcaagca agaattagat agaaggcgtc tattggacct ccctggtggt
    63961 attgtcttaa tcggtgggaa tgccatttta ccaggtatgg ttgagcttgc tcaggaagtc
    64021 tttggcgtcc gtgtcaagct ttatgttcca aatcaagttg gtatccgtaa tccagccttt
    64081 gcgcatgtga ttagtttatc agaatttgcg ggtcaattaa cagaagttaa tcttttggct
    64141 cagggagcga taaaaggtga gaatgactta agtcatcagc caattagttt tggtgggatg
    64201 ctgcaaaaaa cagctcagtt tgtacaatca acgcctgttc aaccagctcc tgctccagaa
    64261 gtagagtcgg tggcgcctac agaaccaatg gcggatttcc aacaagcttc acaaaataaa
    64321 ccgaaattag cagatcgttt ccgtggcttg atcggaagca tgtttgacga ataaagagga
    64381 aaaataaatt atgacatttt catttgatac agctgctgct caaggggcag tgattaaagt
    64441 aattggtgtc ggtggaggtg gtggcaatgc catcaaccgt atggtcgacg aaggtgttac
    64501 aggcgtagaa tttatcgcag caaacacaga tgtacaagca ttgagtagta caaaagctga
    64561 gactgttatt cagttgggac ctaaattgac tcgtggtttg ggtgcaggag gtcaacctga
    64621 ggttggtcgt aaagccgctg aagaaagcga agaaacactg acggaagcta ttagtggtgc
    64681 cgatatggtc ttcatcactg ctggtatggg aggaggctct ggaactggag ctgctcctgt
    64741 tattgctcgt atcgccaaag atttaggtgc gcttacagtt ggtgttgtaa cacgtccctt
    64801 tggttttgaa ggaagtaagc gtggacaatt tgctgtagaa ggaatcaatc aacttcgtga
    64861 gcatgtagac actctattga ttatctcaaa caacaatttg cttgaaattg ttgataagaa
    64921 aacaccgctt ttggaggctc ttagcgaagc ggataacgtt cttcgtcaag gtgttcaagg
    64981 gattaccgat ttgattacca atccaggatt gattaacctt gactttgccg atgtgaaaac
    65041 ggtaatggca aacaaaggga atgctcttat gggtattggt atcggtagtg gagaagaacg
    65101 tgtggtagaa gcggcacgta aggcaatcta ttcaccactt cttgaaacaa ctattgacgg
    65161 tgctgaggat gttatcgtca acgttactgg tggtcttgac ttaaccttga ttgaggcaga
    65221 agaggcttca caaattgtga accaggcagc aggtcaagga gtgaacatct ggctcggtac
    65281 ttcaattgat gaaagtatgc gtgatgaaat tcgtgtaaca gttgttgcaa cgggtgttcg
    65341 tcaagaccgc gtagaaaagg ttgtggctcc acaagctaga tctgctacta actaccgtga
    65401 gacagtgaaa ccagctcatt cacatggctt tgatcgtcat tttgatatgg cagaaacagt
    65461 tgaattgcca aaacaaaatc cacgtcgttt ggaaccaact caggcatctg cttttggtga
    65521 ttgggatctt cgccgtgaat cgattgttcg tacaacagat tcagtcgttt ctccagtcga
    65581 gcgctttgaa gccccaattt cacaagatga agatgaattg gatacacctc catttttcaa
    65641 aaatcgttaa gtaaatgaat gtaaaagaaa atacagaact tgtttttcga gaagttgcag
    65701 aggctagtct gagtgctcat cgagagagtg gttcggtctc tgtcattgca gttaccaagt
    65761 atgtagatgt accgacagcg gaagccttgc ttccgctagg tgttcatcat atcggtgaaa
    65821 atcgtgtaga taagtttctg gaaaaatatg aagctttaaa agatcgagat gtgacttggc
    65881 atttgattgg taccttgcaa agacgtaagg tgaaagatgt cattcaatac gttgattatt
    65941 tccatgcatt ggactcagta aagctagcag gggaaattca aaaaagaagt gaccgagtca
    66001 tcaagtgttt ccttcaagta aatatttcta aagaagaaag caaacacggt ttttcgagag
    66061 aggaactgct ggaaatcttg ccagagttag ccagactaga taagattgaa tatgttggtt
    66121 taatgacgat ggcacctttt gaggctagca gtgagcagtt gaaagagatt ttcaaggcga
    66181 cccaagattt acaaagagaa attcaagaga aacaaattcc aaatatgcct atgaccgagt
    66241 taagtatggg aatgagtcgt gattataaag aagcgattca attcggttcc acttttgttc
    66301 gtataggtac atcatttttt aagtaggaga gaaccatgtc tttaaaagat agattcgata
    66361 gatttataga ttattttacg gaggatgagg attcaagtct cccttatgaa aaaagagatg
    66421 agcctgtgtt tactccagta aattcttcac aggaaccggc tctcccaatg aatcaacctt
    66481 cacagtcggc tggtacaaaa gagaacaata tcaccagact tcatgcaaga caacaggaat
    66541 tggcaaatca gagtcagcgt gcaacggata aggtcattat agatgttcgt tatcctagaa
    66601 aatatgagga tgcaacagaa attgttgatt tattggcagg aaacgaaagt atcttgattg
    66661 attttcagta tatgacagag gtgcaggctc gtcgttgttt ggactatttg gatggagctt
    66721 gtcatgtttt agctggaaat ttgaaaaagg tagcttctac catgtatttg ttgacaccag
    66781 tgaatgttat tgtaaatgtt gaagatatcc gtttaccaga tgaagatcaa cagggtgagt
    66841 tcggttttga tatgaagcga aatagagtac gataatgatt tttttaattc gtatgattta
    66901 taatgcagtg gatatttact ccctgatttt ggtagccttc gctgtcatgt cttggtttcc
    66961 aggtgcctac gaatccagtt taggtcgttg gattgtagcg ttggtgaaac cagtgcttgc
    67021 tcccttgcaa cgcctgcctt tacagatagc gggtcttgat ttatctgttt gggtcgcgat
    67081 tgttttggtt cgatttttag gagaaaacct agtgcgtttt ctggcgatga taggatgaat
    67141 aaagggattt atcagcattt ctccatagaa gatcgtccat ttcttgacaa gggaatggaa
    67201 tggataaaga aggtagaaga tagctatgct ccttttttaa ctccttttat caatcctcat
    67261 caggagaagc tattaaagat tttggccaaa acctatggtc ttgcttgtag cagtagtggg
    67321 gaattcgtct cgagtgagta tgttcgagtt ttattatacc cagattattt ccaaccagag
    67381 ttttcagatt ttgaaatatc tctccaggaa attgtgtatt ccaataaatt tgaacattta
    67441 acgcatgcta agattttagg gacagtcatc aatcaattag ggattgaacg gaaacttttt
    67501 ggagatatcc tagtagatga agaacgggcg cagattatga ttaatcagca gtttcttctt
    67561 ctctttcaag atggactaaa gaaaattggt cgtatacctg tttcgctgga ggaacgtcct
    67621 ttcaccgaga aaatagataa gctagaacag tatcgagaac tggatttatc tgtgtctagt
    67681 tttcgattag atgttctttt atcaaatgtt ttgaaactat ctaggaatca agcaaaccag
    67741 ttgattgaaa agaaacttgt ccaagtaaat tatcatgtgg tagacaaatc agattacact
    67801 gttcaagttg gagacttgat tagtgtgaga aaatttggtc gcttgagatt acttcaagat
    67861 aagggacaaa cgaaaaaaga gaagaaaaaa ataaccgtcc agttattatt aagtaagtga
    67921 ggaatagaat gccaattaca tcattagaaa taaaggacaa gacttttgga actcgattca
    67981 gaggttttga tccagaagaa gtcgatgaat ttttagatat tgtggttcgt gattacgaag
    68041 atcttgtgcg tgcgaatcat gataaaaatt tgcgtattaa gagtttagaa gagcgtttgt
    68101 cttactttga tgaaataaaa gattcattga gccagtctgt attgattgct caggatacag
    68161 ctgagagagt gaaacaggcg gcgcatgaac gttcaaacaa tatcattcat caagcagagc
    68221 aagatgcgca acgcttgttg gaagaagcta aatataaggc aaacgagatt cttcgtcaag
    68281 caactgataa tgctaagaaa gtcgctgttg aaacagaaga attgaagaac aagagccgtg
    68341 tcttccacca acgtctcaaa tctacaattg agagtcagtt ggctattgtt gaatcttcag
    68401 attgggaaga tattctccgt ccaacagcta cttatcttca aaccagtgat gaagccttta
    68461 aagaagtggt tagcgaagta cttggagaac cgattccagc tccaattgaa gaagaaccaa
    68521 ttgatatgac acgtcagttc tctcaagcag aaatggaaga attacaagct cgtattgagg
    68581 tagccgataa agaattgtct gaatttgaag ctcagattaa acaggaagtg gaaactccaa
    68641 ctcctgtagt gagtcctcaa gttgaagaag agcctctgct catccagttg gcccaatgta
    68701 tgaagaacca gaagtagctc cagtgcatcc gacaggtcca acaccagcta cagaaactgt
    68761 tgattcagca ccgggatttg aagcaccgca agaatctgtt acaattttat aagaaatatt
    68821 ctgagaacaa tatcttatcc ttatattccc agcgagcagg agatggtgtg agtcctgcat
    68881 tccctatcga taagattatc ctctcaaact atcaagtctg aatctagtaa gatttgacgt
    68941 tccccacgtt acgggataag agggagaaag actaaaactt tttccgaata aaggtggtac
    69001 cacgattttc gtcctttttg gaagtcgtgg tttttaattt gttattattt ataaaggaga
    69061 taccatgaaa ctcaaagaca cccttaatct tgggaaaact gaattcccaa tgcgtgcagg
    69121 ccttcctacc aaagagccag tttggcaaaa ggaatgggaa gatgcaaaac tttatcaacg
    69181 tcgtcaagaa ttgaaccaag gaaaacctca tttcaccttg catgatggcc ctccatacgc
    69241 taacggaaat atccacgttg gacatgctat gaacaagatt tcaaaagata tcattgttcg
    69301 ttctaagtct atgtcaggat tttacgcacc atttattcct ggttgggata ctcatggtct
    69361 gccaatcgag caagtcttgt caaaacaagg tgtcaaacgt aaagaaatgg acttggttga
    69421 gtacttgaaa ctttgccgtg agtacgctct ttctcaagta gataaacaac gtgaagattt
    69481 taaacgtttg ggtgtttctg gtgactggga aaatccatat gtgaccttga ctcctgacta
    69541 tgaagcagct caaattcgtg tatttggtga gatggctaat aagggttata tctaccgtgg
    69601 tgctaagcca gtttactggt catggtcatc tgagtcagcc cttgctgaag cagagattga
    69661 ataccatgac ttggtttcaa cttcccttta ctatgccaac aaggtaaaag atggcaaagg
    69721 agttctagat acagatactt atatcgttgt ctggacaacg actccattta ctatcacagc
    69781 ctctcgtggt ttgacggttg gtgcagatat tgattacgtt ttggttcaac ctgctggtga
    69841 agctcgtaag tttgtcgttg ctgctgaatt attgactagc ttgtctgaga aatttggctg
    69901 ggctgatgtt caagttttgg aaacttaccg tggccaagaa ctcaaccaca tcgtaacaga
    69961 acacccatgg gatacagctg tagaagagtt ggtaattctt ggtgaccacg ttacgactga
    70021 ctctggtaca ggtattgtcc atacagcccc tggttttggt gaggacgatt acaatgttgg
    70081 tattgctaat aatcttgaag tcgcagtgac tgttgatgaa cgtggtatca tgatgaagaa
    70141 tgctggtcct gaatttgaag gtcaattcta tgaaaaggta gttccaactg ttattgaaaa
    70201 acttggtaac ctccttcttg cccaagaaga aatctctcac tcatatccat ttgactggcg
    70261 tactaagaaa ccaatcatct ggcgtgcagt tccacaatgg tttgcctcag tttctaaatt
    70321 ccgtcaagaa atcttggacg aaattgaaaa agtgaaattc cactcagaat ggggtaaagt
    70381 ccgtctttac aatatgatcc gtgaccgtgg tgactgggtt atctctcgtc aacgtgcttg
    70441 gggtgttcca cttcctatct tctacgctga agatggtaca gctatcatgg tagctgaaac
    70501 tattgaacac gtagctcaac tttttgaaga acatggttca agcatttggt gggaacgtga
    70561 tgccaaagac ctcttgccag aaggatttac tcatccaggt tcaccaaacg gcgagttcaa
    70621 aaaagaaact gatatcatgg acgtttggtt tgactcaggt tcatcatgga atggagtggt
    70681 ggtaaaccgt cctgaattga cttacccagc cgacctttac ctagaaggtt ctgaccaata
    70741 ccgtggttgg tttaactcat cacttatcac atctgttgcc aaccatggcg tagcacctta
    70801 caaacaaatc ttgtcacaag gttttgccct tgatggtaaa ggtgagaaga tgtctaaatc
    70861 tcttggaaat accattgctc caagcgatgt tgaaaaacaa ttcggtgctg aaatcttgcg
    70921 tctctgggta acaagtgttg actcaagcaa tgacgtgcgt atctctatgg atatcttgag
    70981 ccaagtttct gaaacttacc gtaagattcg taacactctt cgtttcttga ttgccaatac
    71041 atctgacttt aacccagctc aagatacagt cgcttacgat gagcttcgtt cagttgataa
    71101 gtacatgacg attcgcttta accagcttgt caagaccatt cgtgatgctt acgcaaactt
    71161 tgaattcttg acaatctaca aggccttggt gaactttatc aacgttgact tgtcagcctt
    71221 ctaccttgat tttgccaaag atgttgttta cattgaaggt gccaaatcac tggaacgccg
    71281 tcaaatgcag actgtcttct atgacattct tgtcaaaatc accaaactct tgactccaat
    71341 ccttcctcac actgcggaag aaatctggtc atatcttgag tttgaagccg aagacttcgt
    71401 ccaattgtca gaattaccag aagttcaaac ttttgctaac caagaagaaa tcttggatac
    71461 atgggcagcc ttcatggact tccgtggaca agcacaaaaa gccttggaag aagctcgtaa
    71521 tgcaaaagtt atcggtaaat cacttgaagc acacttgaca gtttatccaa atgaagttgt
    71581 gaaaactcta ctcgaagcag taaacagcaa tgtagctcaa cttttgatcg tgtctgagtt
    71641 gaccatcgca gaagaaccag ctccggaagc tgcccttagc ttcgaagatg tagtcttcac
    71701 agttgaacgc gctgcaggtg aagtatgtga ccgttgccgt cgtattgacc caacaacagc
    71761 agaacgtagc taccacgcag tcatctgtga ccactgtgca agtatcgtag aagaaaactt
    71821 tgcagatgca gtcgcagaag gatttgaaga gaaataagat tgaaaagtct aggtaaaact
    71881 caatttgaga agaaaagaca actaatttta tagtctatta aacgcattgt atcacgtttt
    71941 tgaacacctg atatgatgcg ttttttattt attttaaaaa tttgcgaggt atgacttttt
    72001 atactcaaca agaatcaaag agaaacttag caagctaaca gtagtaatat aaaataggaa
    72061 tttgatatta gggataagat tggtaaatag tgtaatattt ttacaacaat aaatttatat
    72121 agttatttct ggtttctgaa aagtattata ttatatttta tttcatatta tacaaatttt
    72181 tattttataa tatcagaaaa tacttttttt aaaaagcaaa tatgatacaa ttttatttga
    72241 aaaaaaataa aaaaggagat tttattatga aatcaagtgg tatcgtcggt cttgtgggag
    72301 gaattttact tcttatttgt ccttttgtct tgttgggaac agcggtaaat acagctgcta
    72361 caactcttaa tggaggagct actgcagggg ctttttcagg tgttgcctta ctcttgaatg
    72421 ccttgaagat tgcaaatctt gttcttggta tcattgctat tgtttactat aaaggagata
    72481 agcatgtagg tgcagctccg tctgtactaa tgattgtttc tggtgaagtt agtctcattc
    72541 cattcttagg atgggttggg gggattcttg ctattatcgg aggatctcta ttccttgcaa
    72601 cattgaagaa attcaaatca gaagaataaa aggtatttta gcatgaaaag aacaaaaaag
    72661 tttatcggta taggagtagc tctattatct ctttctcttc tagttgcatg tggaacataa
    72721 agttcaaaga atacttcaac aagtaatgat gagaagacag tagcaacatc caatagttca
    72781 aaagaaacaa tcactttcga tacaccggtt gtaacagacg atgcgattga atcaatacgc
    72841 acttatgcag attatataga tctttataaa aatatttttg atgattattt tactaaagct
    72901 gaggaaggtt tcaaaggcac aactatggaa aataatgact cgtttactaa actaaaagag
    72961 tcaactcaaa aattattcga tgcgcagaaa aaaaggttaa ataatgaaga tagaatagaa
    73021 acaaccaaaa acaatgtgat tgccaaacat tgtcaaacag tcctttcctt tttggttttg
    73081 actagctttt ttgtgaaaaa ttgtgtaaaa tagaatagat aaacgagggg aaacctcgga
    73141 aaatttaaag gagaatccat ctaatggtaa aattggtttt tgctcgccac ggtgagtctg
    73201 aatggaacaa agctaacctt ttcactggtt gggctgatgt tgatttgtct gaaaaaggta
    73261 cacaacaagc gattgacgct ggtaaattga tcaaagaagc tggtatcgaa tttgaccaag
    73321 cttacacttc agtattgaaa cgtgctatca aaacaactaa cttggctctt gaagcttctg
    73381 accaattgtg ggttccagtt gaaaaatcat ggcgcttgaa cgaacgtcac tacggtggtt
    73441 tgactggtaa aaacaaagct gaagctgctg aacaatttgg tgatgagcaa gttcacatct
    73501 ggcgtcgttc atacgatgta ttgcctccaa atatggaccg tgatgatgag cactcagctc
    73561 acacagaccg tcgttatgct tcacttgacg actcagtcat tccagatgct gaaaacttga
    73621 aagtgacttt ggaacgtgct cttccattct gggaagataa aatcgctcca gctcttaaag
    73681 atggtaaaaa cgtattcgta ggagctcacg gtaactcaat ccgtgccctt gtaaaacaca
    73741 tcaaaggttt gtcagatgat gagatcatgg acgtggaaat ccctaacttc ccaccattgg
    73801 tattcgaatt cgacgaaaaa ttgaacgttg tttctgaata ctaccttgga aaataaaaaa
    73861 ttgtaagcct aggattgatt tctaggcttt ttatgttagt atggaagtat gataaggaat
    73921 aaaaaacaag attatgtact ggcctacaag caaccagctt caaccactta catgggttgg
    73981 gaagaagaag ctttaccgat aggcaatggt tctttaggag caaaagtatt tggccttata
    74041 ggggctgaac ggattcaatt taatgaaaaa agtctctggt ctggaggtcc acttcctgat
    74101 agttcagatt atcagggtgg aaatcttcag gatcagtatg tttttttagc tgagattcgg
    74161 caggctttgg agaagagaga ttacaatctg gctaaggaac tggctgagca gcacctaatt
    74221 gggccaaaaa cgagtcaata tgggacctat ctgtcttttg gggatattca cattgagttc
    74281 agccagcaag gtacgacttt gtctcaggtg acggactatc agagacagct gaatattagt
    74341 aaggcacttg cgacgacttc ttatgtctat aagggaacgc gatttgaacg taaagctttt
    74401 gcgagttttc cagatgatct cttgattcaa tgttttacta aggaagggtt ggaaactcta
    74461 gattttacta tagaactatc cttgacctgt gatttggctt ctgatggaaa gtatgagcag
    74521 gaaaaatctg attacaagga gtgtaagttg gatattactg attctcatat cttgatgaag
    74581 ggaagagtta aggataatga tctgcggttt gctagttatc tagcttggga aacggatgga
    74641 gatattagag tttggtcaga tagggttcag atatcaggag ccagttatgc caatctcttc
    74701 ttggccgcta agacggattt tgcccaaaat cctgctagca attatcgcaa gaaactagat
    74761 ttagagcaac aggtgataga cttggtggac acagctaaag aaaagggcta tacccaattg
    74821 aaatcaaggc atatcgagga ctaccaagcc ttattccagc gtgttcaatt ggatttggaa
    74881 gctgatgttg acgcatccac tacagatgat ttgttaaaaa attataagcc acaagaaggg
    74941 caggctttgg aggagctgtt cttccagtat ggacggtatt tattgattag ttcgtccaga
    75001 gactgcccag atgctctacc agctaaccta cagggagtct ggaatgcggt cgacaatcct
    75061 ccttggaatt cggactatca cttaaatgtc aatctgcaga tgaattattg gccagcctat
    75121 gttaccaatc tcctagagac ggtctttcca gtcatcaact atgtagatga tttgcgtgtc
    75181 tatggtcgtc tagcggctgt aaagtatgca ggaatcgtct ctcaaaaagg tgaggaaaat
    75241 ggttggttgg ttcatactca agcgactccc tttggttgga cggcacctgg ttgggattac
    75301 tattggggtt ggtcaccagc tgccaatgcg tggatgatgc aaaccgttta tgaagcctat
    75361 tcattttata gggaccaaga ctatctcagg gagaaaattt atcccatgtt gagggaaacg
    75421 gttcgttttt ggaatgcctt tttacataag gatcagcagg cgcagcgttg ggtctcttct
    75481 ccgtcttatt ccccagaaca tgggccgatt tcgattggca atacctatga ccaatctctg
    75541 atttggcagt tatttcatga ttttattcag gctgctcagg aattgggact ggatgaggac
    75601 ttgttgactg aggttaagga gaagtctgat ttactaaatc ctttgcaaat cactcaatct
    75661 ggtcgaatca gggagtggta tgaggaggaa gagcagtatt ttcaaaatga gaaagtggag
    75721 gcccagcatc ggcacgcttc ccatctagtg ggactctatt ctggcaatct ctttagctac
    75781 aagggacaag agtatattga agcggcgcgt gctagcctca atgatcgtgg agatggcggc
    75841 acaggctggt ccaaggctaa taagatcaat ctctgggcgc gtttgggaga tggcaatcga
    75901 gcccataaat tattggcaga gcagttaaag acatccacct tgccaaatct ttggtgtagc
    75961 catcctcctt ttcagataga tggtaatttt ggtgctacta gtggcatggc agaaatgtta
    76021 ctccagtctc atgcagctta tctggtacct ctagctgccc tacctgatgc ttggtcaaca
    76081 ggttctgttt caggcttaat ggcacgtgga cattttgaag tgagcatgag ctgggaagat
    76141 aaaaaactct tacagttgac cattttatca aggagtggag gagatttgcg agtttcttat
    76201 ccagatattg agaagagtgt gattaaaatg aatcaagaaa aaataaaagc gaaatgcatg
    76261 gggaaagatt gtatttcggt ggcaacagca gaaggtgatc ttgttcaatt ttatttttaa
    76321 gaagatgtta taaggcagta atttgaaact tccttttaat aaggatttga gaatataggc
    76381 agtttccaac tagttgaaaa agcgttataa tgataatagg aagtaatact caatgaaaat
    76441 caaagagcac aaactaggaa gctagccgca ggttgctcaa aacagtgttt tgaggttgta
    76501 gatggaagct gacgtggttt gaagagagat tttcgaggag tataatttgt ttgatagagg
    76561 gtgggtctga tggcttatat tgagatgaaa cactgttaca agcgttatca ggttggggac
    76621 acggagattg tggccaatcg tgatgtgaat tttgagattg aaaaggggga gctggttatt
    76681 atccttggtg cttcaggtgc aggcaagtca acagttctta accttcttgg gggaatggat
    76741 accaatgatg aaggggaaat ctggattgat ggtgttaata ttgcggatta tagttcccac
    76801 cagcgtacca attaccgtag aaatgatgtg gggtttgttt ttcagtttta taatctagtt
    76861 tctaatctga cagctaagga aaatgtggaa ctggcttctg aaattgtgac agatgccttg
    76921 aatcctgatc aggtcttgac agatgtaggt ctggctcatc gtctcaataa ctttccagcc
    76981 cagctttctg gaggggagca acagcgagtc tccattgcac gcgcggtagc caaaaatcct
    77041 aaaattctcc tttgtgatga accgactgga gccttggatt atcagacggg caagcaggtt
    77101 ttgaaaattc tccaagacat gtctcgtcaa aagggagcga cggtgatcat cgtgactcat
    77161 aatggagctt tggcgcccat tgctgatcgc gtgattcaaa tgcacgatgc cagtgtcaag
    77221 gatgtggtgc tcaaccagca tcctcaggat attgacagtt tggagtacta gcatgatcaa
    77281 gcgaaaaact tattggaagg acttaattca gtccttcaca ggctccaagg ggcgtttttt
    77341 atccatcttg atcctgatga tgttgggatc tctagcctta gtaggcctca aagtaaccag
    77401 tcccaacatg gaggcgacag ctaatgctta tttaacaact gctcaaacct tggatttggc
    77461 agtcatgtct aactatggct tggatcaagc agaccaagaa gaactaaaac agacggaggg
    77521 cgcagaggtc gagtttggct atttgacaga tgtgactatg gataatgggc aggatgccat
    77581 tcggctgtac tccaaaccag agcgaatttc aacctttcag ctaagaaagg gacgacttcc
    77641 tcagtcagac aaggaaatcg ctttggccac tcatttgcaa ggccaataca gcgtgggaca
    77701 ggagattagt tttaaagaaa aagaagaggg tcattcctct ttaaaagacc atacttatac
    77761 cattactggt tttgtggatt cggctgaaat cctctcccag cgagatatgg gctacgcagg
    77821 aagtggaagt gggactctga cagcctatgg ggtgatttta cctagtcagt ttgatcagaa
    77881 agtctacaat atagctcgtt tgaaatatca agatttagca ggtttaaatg ccttttcatc
    77941 agcttatgaa gaaaaatcca agcaacatca ggaagacctt gaacaaactt tatcagataa
    78001 tggcaaggta cgtctgcaac ttttgaaaaa agaaggacaa gagtctctag acaaggggca
    78061 agagaccctt gacaaggctc agactaattt gcaggaaggc aagcgtcgtt tagcagctgc
    78121 tcaagctcgt atacaggctc aagaaagtca actagccttg tttcctcaag ttcagagaga
    78181 gcaggctagt gctcaactta cccaagccaa gcaggaattg ggcaaggaag aggataaact
    78241 aaagcaagct gaacaaaatc tagcccaaga aaaggaaaag ttagaaaaac atcagcaagt
    78301 cttggacgat ttggcggagc caaggtatca ggtctataat cgtcagacca tgccaggtgg
    78361 tcagggctat cttatgtata gcaatgcttc atccagtatt cgagcagtgg gcaatatctt
    78421 tcctgtggta ctttatgccg tagcatccat ggtgaccttt acgaccatga ctcgctttgt
    78481 agacgaagag cgaactcatg cagggatttt taaggccttg ggttaccgta gtaaggatat
    78541 tatcaccaag tttctccttt atggactagt agctgggact gtcggaacgg ctctaggtag
    78601 tatacttggt cattatttgc tagccagtgt aatttcaagt gtcattacaa aaggcatggt
    78661 ggtgggagaa acccagattc agttctattg gacctatagc ttactagctc ttgtcttgag
    78721 ctggttggcg agtgtgttac cagcctatct ggtggcttgg agggaacttc atgccgaagc
    78781 agcccagctt ctacttccta aacctcctgt caaaggagct aaaatcttat tggagcgtat
    78841 cggttttatc tggcgtcgtc tcagttttac tcataaggta acagcccgca acatctttcg
    78901 ttataagcag agaatgttga tgacaatctt tggtgtggca ggttctgtag ctctgctctt
    78961 tgcaggtttg ggaatccaat cttctgtagc aggagttccg tctaaacagt ttcaacaaat
    79021 ccaacagtat cagatgcttg tctctgaaaa tcctagtgcg accaatcagg acaaggtaga
    79081 gctagcagaa gtgttgaaag ggcaggagat actagcctac cagaaaatct attctaaaac
    79141 gctagacaag gatttcaaag gcaaggctgg tcttcaaaac attactctta tgatgataga
    79201 gaaggaagat ttgactccct ttatccatct tcaacatcat cagcaggagc tgacattaaa
    79261 agatggcatc gttattacag ctaaactcgc ccagctggca ggtgtcaagg ttgggcagac
    79321 tttagaaatt gaaggtaagg aactaaaggt cgctgctatt actgagaact acgttggtca
    79381 ctttatttat atgagtcagg ctagctatga gcaactttac ggacagctac cccaagccaa
    79441 cacttatctg gtctcattaa gggataccag tgcaactagt atcgaaagtc aggcgggctt
    79501 gcttatgaat caatctgcgg tgtccagcgt tgtccaaaat gcttcagcca ttcgactcta
    79561 tcgctagctc actcaatcag accatgacca tcttggtcat cgtatcagtt ctattagcta
    79621 ttgtcatcct ttacaatctg accaatatca acgtagctga gagaatccgt gaactctcca
    79681 ctatcaaggt tctcggtttt cataataatg aagtcaccct ctacatttac cgtgagacga
    79741 ttgtgctgtc ccttgtggga atcgtacttg gtctggtagc tggtttctat ttacaccaat
    79801 ttttgattca aatgatttcg cctgcgacta ttctctttta tccgcaggta ggctgggaag
    79861 tctatgtaat cccagtggca acagtaagca tcattttgac cttgcttggt ttcttcgtca
    79921 attattatct gagaaaggtt gatatgttag aagccctgaa atctgtagag taaggtagtt
    79981 atttttagct gattgaactt ctatttacta atattcaaaa atcctctgtc acaaagagca
    80041 gggagctctt tgtgacagag gattttttct atagggcttt agcagctgca attgcggctt
    80101 cgaagtttgg ctcagaattg atattatcca cgtattcaac gtagcgaatc gtattgtcag
    80161 tatcgaggac aaagactgcg cgtgctaata ggtgccattc gttgatcaag agggcataat
    80221 cgcgcccgaa agaatggtca aagtagtctg aaagcataat ggcattgtca aggccttcag
    80281 caccgcacca acgtttttga gcaaaaggta ggtccattga aacagtcaat acgaccgtgt
    80341 tgtccagtcc agccaattct tcattaaaac gacgtgtttg agttgagcag atgcctgtat
    80401 cgatagaagg aacgacactc aagacttttt tcttgccatc aaaatcagcc agagattttt
    80461 tagaaagatc tgttgtagta agagaaaaat caagcgcctt gtcgccgact tgtagttgtt
    80521 tacctgtaaa gctcacagga tttccgagaa aagttaccat aggatactcc aatctttttt
    80581 cttccattgt atctgaaaca gtcagaattt tccaatgatt tgaccggaaa tgtgggcata
    80641 gaaaaaacgc cagctcacat gagaatgacg tttttcagag gcttattttg ccaatccttc
    80701 agcaatcttg tcaaggttgt atttcatcat gctgtagtag ctgtcgcctt ctttaccttg
    80761 ttctgcgata gagtcagtaa agatttgtgc gtagattggg atgtttgtgt cttgagaaac
    80821 agttttcatt ggacggtcat ccacacttga ttctacaaag agtgatggaa cttttgtttg
    80881 gcgaagtttt tcaaccaagg tcttgatttg ttcaggagtt ccttcttctt cagtattgat
    80941 ttcccagatg taagcacttg ggacaccata ggctttagag aagtatttga atgctccttc
    81001 gctggttaca atgagtttct tttcagcagg gatcttatta aatttatcct tactttcttt
    81061 atcaagtttg tctaacttat cagtatattc tttgagattt ttttcataga attctttatt
    81121 gttagggtct ttggcgctca attgtttggc gatattttta gcaaaaataa taccgttttc
    81181 aaggttaagc caagcgtgtg ggtcttcttt tcctttttca ttttgacctt caaggtagat
    81241 aacatcaacg ccgtcgctga ctgcgaagta gtctttgttt tcagttttct tggcattttc
    81301 taccaatttt gtaaaccaag cattgccacc tgtttcaagg ttgataccgt tatagaaaat
    81361 caaatcagcc tcagaagttt tcttaacgtc ttcaggaagt ggttcgtatt cgtgtgggtc
    81421 ttgcccaatc ggaacgatac tatgaaggtc aattttgtca ccagcaatat ttttagtaat
    81481 atcagcgatg attgagtttg tagcaacaac ttttagtttt tgaccagaag ttgtatcttt
    81541 ttttccgcta gcacatgcta caagaatgat tgcagaaaga aagagaacga gtaatgtacc
    81601 taattttttc attagatcct ccaaattatt agggctttgc cccttatttt aacaaatgtt
    81661 tatttttcag tttcaaatat cgttgtttgg gagcgataaa gaagctaatg agaaagaaac
    81721 tagcagctgt aagcacgata ctagaacctg ccgcaacatt aaaactatag ccaataaaga
    81781 gtcccaaaac tgaagcagta gctccgaagg ttgaggaaag gaaaatcatg cttttcagac
    81841 tattagcata cagataagca gttgcagctg gggtaatcag catggctaca atcaggatag
    81901 ttccgacact ttgcatggct gtcacagaca cgagagtcag gagtaccatg agaaggtagt
    81961 gatagaaatt gacaggcatt cccatggctt tagccaagag ttcatcaaag gaagttatca
    82021 agagttgctt gaagaaaatc cagattaaca agaggatggc tgcccccaca cccatagtaa
    82081 taaacatatc cgtatcttgg acggccagga tattaccaaa aaggatatgg aaaaggtcag
    82141 ttgaactttt agcgacacca atcaagatga taccgagggc taagaaagaa gaaaaggtaa
    82201 tgccgatggc ggtatcgctt ttgataatcg agtttccttt gatgtaggta atgatgatgg
    82261 cagctagcaa tccaaagaca atggctccga taaagaagtc aaggcccaag atgaaggaga
    82321 gggctacacc tggtaagaca gcatgtgaaa tggcatctcc catgagtgac atcccgcgta
    82381 gaataatgaa acatcccaca gctccagcta caatcccgac gacaatagct gttatcaagg
    82441 cattttgtag gaaatggaat ttttgcaatc catcgataaa ttctgcaatc ataggtcacc
    82501 tccattgaaa aagagttgat taccgtaagc ttcttttaga ttggtttcgg taaaagtttc
    82561 ttttgttgga ccaaaggcaa tcacttctcg attgacaagt aagacttgat cgaagtagtg
    82621 gggaatcttg ctgaggtcgt ggtgaacgat gagaaccgtc ttcccagctt ttttcaaatc
    82681 tctcagcgta ttcatgatga tttcctcact gacagagtca atcccagcaa agggttcatc
    82741 caagaggata tagtcggctt cctgcaccaa acatctggca atcaaaaccc gctggaattg
    82801 acctccagac agttgactaa tttgacgttc agcgtagtca gctaggccga cgatttcaag
    82861 ggcctcttgc actttcttcc aatgtttagc ctttaaactt cgaaagagag gaatagaggg
    82921 aaatagtcct aacgagacgc attccttgac cttgatggga aagttgtagt cgatattgat
    82981 tttttgttcg acataggcaa ttcggtgtaa ggatttttta acttccttgt catcgagaaa
    83041 tgcctgacct tgatgtggga taattcccag catacctttt aatagtgttg atttcccagc
    83101 gccgtttgga ccaatgatgc cggtaattgt tggtccatgg agcactagtg aaatatcctt
    83161 aagtgccaac gtttctttgt aggagacact gaggttttcg atacgtatca taaacttgta
    83221 ttcctcctgt ctcttaatat acattaaaaa aaaaattaag tcaagttaat ttttgaaaaa
    83281 attaaaataa taactgaaaa ataga
//
LOCUS       DAVBCN010000002        67281 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVBCN010000002 DAVBCN010000000
VERSION     DAVBCN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 67281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67281
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.002"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..102
                     /locus_tag="VQ497_000075"
     CDS             <1..102
                     /locus_tag="VQ497_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET2516448.1"
                     /translation="VPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
     gene            508..1314
                     /locus_tag="VQ497_000076"
     CDS             508..1314
                     /locus_tag="VQ497_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2516449.1"
                     /translation="MILKDINFALNKGEIVGLVGRNGVGKSTLMKILVQNNQPTSGNI
                     ISSDNVGYLIEEPKLFLSKTGLENLKYLSNLYGVDYNQERFRSLIQELDLTQSINKKV
                     KTYSLGTKQKLALLLTLVTEPDILILDEPTNGLDIESSQIVLAVLKKLALNENVGILI
                     SSHKLEDIEEICERVLFLENGLLTFQKVGKDSHNFLFEIAFSSATDRDIFITKQEFGD
                     IVQEEGLRITMSGNIQSSELFKFFNENSIKVVDFETKKETLKDIYLNRSK"
     gene            1606..2048
                     /locus_tag="VQ497_000077"
                     /pseudo
     CDS             1606..2048
                     /locus_tag="VQ497_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640911.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2438..2647
                     /locus_tag="VQ497_000078"
     CDS             2438..2647
                     /locus_tag="VQ497_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2516450.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            2637..3125
                     /locus_tag="VQ497_000079"
     CDS             2637..3125
                     /locus_tag="VQ497_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516451.1"
                     /translation="MKNRFYYYQLLDEREEQLINKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            3241..4701
                     /locus_tag="VQ497_000080"
     CDS             3241..4701
                     /locus_tag="VQ497_000080"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET2516452.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            4698..5522
                     /gene="nadE"
                     /locus_tag="VQ497_000081"
     CDS             4698..5522
                     /gene="nadE"
                     /locus_tag="VQ497_000081"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HET2516453.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            5920..6471
                     /locus_tag="VQ497_000082"
     CDS             5920..6471
                     /locus_tag="VQ497_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2516454.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDIEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            7171..7878
                     /locus_tag="VQ497_000083"
     CDS             7171..7878
                     /locus_tag="VQ497_000083"
                     /inference="COORDINATES: protein motif:HMM:NF019142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2516455.1"
                     /translation="MFRRNLNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGGIIVK
                     VATKPKVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGHVTE
                     QVTVDRKQATDTVIKVPAKSKVEEVLVPCATKYEADNDLSAGQEQEITLGKNGKTVTT
                     ITYDVDGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDDPTLDKSQE
                     VEEVGEIGKLLLLQSIL"
     gene            7932..8321
                     /locus_tag="VQ497_000084"
     CDS             7932..8321
                     /locus_tag="VQ497_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2516456.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(8992..10020)
                     /gene="queA"
                     /locus_tag="VQ497_000085"
     CDS             complement(8992..10020)
                     /gene="queA"
                     /locus_tag="VQ497_000085"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET2516457.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIINMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDSMFIY"
     gene            10171..10878
                     /locus_tag="VQ497_000086"
     CDS             10171..10878
                     /locus_tag="VQ497_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HET2516458.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            11018..11194
                     /locus_tag="VQ497_000087"
     CDS             11018..11194
                     /locus_tag="VQ497_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET2516459.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            11324..12259
                     /locus_tag="VQ497_000088"
     CDS             11324..12259
                     /locus_tag="VQ497_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HET2516460.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLSETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            12252..13040
                     /locus_tag="VQ497_000089"
     CDS             12252..13040
                     /locus_tag="VQ497_000089"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET2516461.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            13041..13424
                     /locus_tag="VQ497_000090"
     CDS             13041..13424
                     /locus_tag="VQ497_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HET2516462.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            13440..13829
                     /locus_tag="VQ497_000091"
     CDS             13440..13829
                     /locus_tag="VQ497_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET2516463.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            13913..15043
                     /locus_tag="VQ497_000092"
     CDS             13913..15043
                     /locus_tag="VQ497_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HET2516464.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            15048..15785
                     /locus_tag="VQ497_000093"
     CDS             15048..15785
                     /locus_tag="VQ497_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HET2516465.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIVEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEESISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            15796..16569
                     /locus_tag="VQ497_000094"
     CDS             15796..16569
                     /locus_tag="VQ497_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HET2516466.1"
                     /translation="MKYKGYLIDLDGTIYKGKDQIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            16559..17176
                     /locus_tag="VQ497_000095"
     CDS             16559..17176
                     /locus_tag="VQ497_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HET2516467.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            17386..17787
                     /locus_tag="VQ497_000096"
     CDS             17386..17787
                     /locus_tag="VQ497_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HET2516468.1"
                     /translation="MITLFLSPSCTSCRKAKTWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            17787..18065
                     /locus_tag="VQ497_000097"
     CDS             17787..18065
                     /locus_tag="VQ497_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HET2516469.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGKGKISLEK"
     gene            18055..18828
                     /locus_tag="VQ497_000098"
     CDS             18055..18828
                     /locus_tag="VQ497_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET2516470.1"
                     /translation="MKSKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLASFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            18829..20133
                     /locus_tag="VQ497_000099"
     CDS             18829..20133
                     /locus_tag="VQ497_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HET2516471.1"
                     /translation="MKFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEEFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            20707..21585
                     /gene="pstS"
                     /locus_tag="VQ497_000100"
     CDS             20707..21585
                     /gene="pstS"
                     /locus_tag="VQ497_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET2516472.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            21585..22502
                     /gene="pstC"
                     /locus_tag="VQ497_000101"
     CDS             21585..22502
                     /gene="pstC"
                     /locus_tag="VQ497_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET2516473.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            22492..23376
                     /gene="pstA"
                     /locus_tag="VQ497_000102"
     CDS             22492..23376
                     /gene="pstA"
                     /locus_tag="VQ497_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET2516474.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            23387..24190
                     /gene="pstB"
                     /locus_tag="VQ497_000103"
     CDS             23387..24190
                     /gene="pstB"
                     /locus_tag="VQ497_000103"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2516475.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            24203..24961
                     /gene="pstB"
                     /locus_tag="VQ497_000104"
     CDS             24203..24961
                     /gene="pstB"
                     /locus_tag="VQ497_000104"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2516476.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            24973..25626
                     /gene="phoU"
                     /locus_tag="VQ497_000105"
     CDS             24973..25626
                     /gene="phoU"
                     /locus_tag="VQ497_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET2516477.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            25802..26599
                     /locus_tag="VQ497_000106"
     CDS             25802..26599
                     /locus_tag="VQ497_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET2516478.1"
                     /translation="MFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVVATSPDY
                     APFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEILSMSFDNVLTSLQTGKADLAVA
                     GISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGTVPESMV
                     KEQLPKAQLTSLTNMGEAVNELQAGKIDAVHMDEPVALSYAAKNAGLAVATVSLKMKD
                     GDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(26619..27890)
                     /locus_tag="VQ497_000107"
     CDS             complement(26619..27890)
                     /locus_tag="VQ497_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET2516479.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            28032..28763
                     /gene="budA"
                     /locus_tag="VQ497_000108"
     CDS             28032..28763
                     /gene="budA"
                     /locus_tag="VQ497_000108"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HET2516480.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFLHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            28773..29525
                     /locus_tag="VQ497_000109"
     CDS             28773..29525
                     /locus_tag="VQ497_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HET2516481.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWFEKKR
                     SLEKADALAYGLGHGGLELIFLGLTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLTAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            29637..30587
                     /gene="murB"
                     /locus_tag="VQ497_000110"
     CDS             29637..30587
                     /gene="murB"
                     /locus_tag="VQ497_000110"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET2516482.1"
                     /translation="MSVREKMLEILEGIDIRFQEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR"
     gene            30646..31803
                     /locus_tag="VQ497_000111"
     CDS             30646..31803
                     /locus_tag="VQ497_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2516483.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            31784..32590
                     /locus_tag="VQ497_000112"
     CDS             31784..32590
                     /locus_tag="VQ497_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2516484.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            32587..33360
                     /locus_tag="VQ497_000113"
     CDS             32587..33360
                     /locus_tag="VQ497_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2516485.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLAPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            33357..34427
                     /locus_tag="VQ497_000114"
     CDS             33357..34427
                     /locus_tag="VQ497_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2516486.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDS
                     AYAFINFMLKPENALQNAEYVGYSTPNIPAKELLPEETKEDKAFYPDVETMKHLEIYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            34680..34919
                     /locus_tag="VQ497_000115"
     CDS             34680..34919
                     /locus_tag="VQ497_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516487.1"
                     /translation="MKMSTFFNKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILEK"
     gene            35194..35679
                     /locus_tag="VQ497_000116"
     CDS             35194..35679
                     /locus_tag="VQ497_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HET2516488.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLLDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            35701..38319
                     /gene="alaS"
                     /locus_tag="VQ497_000117"
     CDS             35701..38319
                     /gene="alaS"
                     /locus_tag="VQ497_000117"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET2516489.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLISPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNTSQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKIVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(38409..39863)
                     /locus_tag="VQ497_000118"
     CDS             complement(38409..39863)
                     /locus_tag="VQ497_000118"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HET2516490.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKEQGIQPMADVVL
                     NHKAAADNREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            40042..40791
                     /locus_tag="VQ497_000119"
     CDS             40042..40791
                     /locus_tag="VQ497_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2516491.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSAPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVEN
                     LRKDHPDQFLESIYLSLAGRKEEVADASQGH"
     gene            40772..42406
                     /locus_tag="VQ497_000120"
                     /pseudo
     CDS             40772..42406
                     /locus_tag="VQ497_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            43091..44254
                     /locus_tag="VQ497_000121"
     CDS             43091..44254
                     /locus_tag="VQ497_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET2516492.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDERRFIVMDVFEYF
                     KYAKRKNLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSCQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            44251..44928
                     /locus_tag="VQ497_000122"
     CDS             44251..44928
                     /locus_tag="VQ497_000122"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HET2516493.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            44918..45772
                     /locus_tag="VQ497_000123"
     CDS             44918..45772
                     /locus_tag="VQ497_000123"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET2516494.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKKMPTEEIW
                     QSLTEKYQ"
     gene            45791..46858
                     /locus_tag="VQ497_000124"
     CDS             45791..46858
                     /locus_tag="VQ497_000124"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET2516495.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVENILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWKVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            46868..48034
                     /gene="aroC"
                     /locus_tag="VQ497_000125"
     CDS             46868..48034
                     /gene="aroC"
                     /locus_tag="VQ497_000125"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET2516496.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            48044..49147
                     /locus_tag="VQ497_000126"
     CDS             48044..49147
                     /locus_tag="VQ497_000126"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HET2516497.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            49158..49496
                     /locus_tag="VQ497_000127"
     CDS             49158..49496
                     /locus_tag="VQ497_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HET2516498.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            49588..50871
                     /gene="aroA"
                     /locus_tag="VQ497_000128"
     CDS             49588..50871
                     /gene="aroA"
                     /locus_tag="VQ497_000128"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET2516499.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            50864..51340
                     /locus_tag="VQ497_000129"
     CDS             50864..51340
                     /locus_tag="VQ497_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET2516500.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRHVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIAADKDNQRPLFLNNSKEELVAIFQERQVWYEEVASRVLDVTKLSPEEIIEELR"
     gene            51337..52185
                     /gene="pheA"
                     /locus_tag="VQ497_000130"
     CDS             51337..52185
                     /gene="pheA"
                     /locus_tag="VQ497_000130"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET2516501.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYIDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            52182..53456
                     /gene="psr"
                     /locus_tag="VQ497_000131"
     CDS             52182..53456
                     /gene="psr"
                     /locus_tag="VQ497_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HET2516502.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            53735..54580
                     /locus_tag="VQ497_000132"
     CDS             53735..54580
                     /locus_tag="VQ497_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2516503.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLTLDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            54570..55673
                     /locus_tag="VQ497_000133"
     CDS             54570..55673
                     /locus_tag="VQ497_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET2516504.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDLEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQIIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            55675..56661
                     /locus_tag="VQ497_000134"
     CDS             55675..56661
                     /locus_tag="VQ497_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2516505.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQIYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            56677..58110
                     /locus_tag="VQ497_000135"
     CDS             56677..58110
                     /locus_tag="VQ497_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HET2516506.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFIAKMLTKEN"
     gene            58139..59173
                     /locus_tag="VQ497_000136"
     CDS             58139..59173
                     /locus_tag="VQ497_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET2516507.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            59373..60110
                     /gene="mecA"
                     /locus_tag="VQ497_000137"
     CDS             59373..60110
                     /gene="mecA"
                     /locus_tag="VQ497_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HET2516508.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            60261..61547
                     /locus_tag="VQ497_000138"
     CDS             60261..61547
                     /locus_tag="VQ497_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET2516509.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            61549..62418
                     /locus_tag="VQ497_000139"
     CDS             61549..62418
                     /locus_tag="VQ497_000139"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET2516510.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTKGVRVEAK"
     gene            62713..63651
                     /gene="msrB"
                     /locus_tag="VQ497_000140"
     CDS             62713..63651
                     /gene="msrB"
                     /locus_tag="VQ497_000140"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET2516511.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            63709..65433
                     /locus_tag="VQ497_000141"
     CDS             63709..65433
                     /locus_tag="VQ497_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2516512.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFLSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENLPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            65435..67183
                     /locus_tag="VQ497_000142"
     CDS             65435..67183
                     /locus_tag="VQ497_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2516513.1"
                     /translation="MKRQTVNQTLKRLVVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKELSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTISRVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
BASE COUNT        19574 a        12512 c        15145 g        20050 t
ORIGIN      
        1 gtcccacaac ccgttcaatc aggagacatg gttcgagctc ttaaagaggg gcttatcaat
       61 ctttataagg aagatggaac cagcgtcaca gttcgtgctt aatgtagttg tttagtttta
      121 aaaaattatg caaagctcca tatacaacac ttaaacgaga ttaaagaatg gcgaaatccc
      181 ttgatgcgca agagattagc tgtctttttt attttttaag tgataaagtc ggagcttagg
      241 tatcaaagcc tatcaaatta aacaaagaag cgatgtctta gatattttga aaaaaattaa
      301 taagcagaaa actctctatt attttgttgt agagagtttt ttgttaataa aattttacaa
      361 aatgacattt atatattgca tcaagttaga tatatgatat aatattgtta aaaagaggcg
      421 caacttttta aactcaataa gaatcaaaga gtaaaccatg aatattaatg taggaataaa
      481 aatgtaagta agcattatgg tcattcaatc attctcaaag atataaattt tgcacttaac
      541 aagggtgaaa ttgttggtct agtagggaga aatggagttg gtaagagtac gttgatgaaa
      601 attcttgttc aaaataatca accgacttca ggtaatatta taagcagtga taatgttggg
      661 tatttaatcg aagaaccaaa attattttta tctaaaacag gtttagagaa tttaaaatat
      721 ttgtcaaatt tatatggtgt tgactacaat caagaaagat ttagaagttt gatccaagag
      781 ttagatttga ctcagtctat taataaaaaa gtaaagacct attctttggg tacaaaacaa
      841 aaattagctt tgcttctaac tctcgttacg gaacctgata tattgatttt agatgaaccg
      901 actaatggtt tagatattga atcatcacaa atagttttag cggttctaaa aaaattagct
      961 ttaaatgaaa acgtgggaat tttaatatcg agtcataaat tagaagacat tgaagaaatt
     1021 tgtgagagag ttcttttctt ggagaacggg cttttgacat ttcaaaaagt aggaaaagat
     1081 agtcataatt tcttgtttga gatagctttt tcatcagcta cagatagaga cattttcatt
     1141 accaaacaag aatttgggga tattgttcag gaagagggat tgagaattac tatgtctggg
     1201 aatattcaaa gtagtgagct ttttaaattt tttaacgaaa actctattaa agtagttgat
     1261 tttgaaacta aaaaagagac gcttaaagat atttacctaa atcgttcaaa ataaaggaag
     1321 gttataatca tgaaattaaa taaattgaat tttcttaagg aaaatataag aaatttatat
     1381 tcatcaggcg taatatatct cagtttgctt atctcgttta taccgccgat attgcttaca
     1441 ttctttattc taaagactta agggacatcg cttggtatta agcatatttc aaacttttat
     1501 gctatgctcg gtatgttaat ggctgttata catgctaatc gagtcattag tagagatttt
     1561 tcccacaata cggtaagttt gttttataat caacagaaga atcggatgat ttacgtcttg
     1621 tctaattttc tatatgccat ctcagcttcc attatttatg ctttgaatgg cattgtgtta
     1681 ctagtcatag taagtaaatt gggtattcca ggtgatttag gattagattt tatagtagct
     1741 attgtagtca atacaatttt gttagtcctg ttttattttc tattatctta cattttctat
     1801 ttatacaaat tgaaaagtgg cttggtattt tagtagcttt actactcttt atccctaata
     1861 tattaaatac gatgatgaat actagtaatg atttgtttat caaagcaatt gaacttcttc
     1921 ctttttattc tttacctgta tttgtggctt caaatacgat gtctattagt cagtatcttg
     1981 tggcaatcac tacaatcatt ttattgtact ttttcactct caagaaaagc aagaagtatt
     2041 cattttagtt tcatttagta ttattttgca tacctaaaat acagtaaaaa atcagtcata
     2101 ttggtatgct cctgctttca ctattcaaca cgtttttgac ttatactagg ctcatttcca
     2161 aaagcattat ataatagtga tatgaaacca actaaactaa acaagaaata taagcaataa
     2221 aaactcgttt aaaagatcct actaaagcta atgctaaata aaaataaaaa taaaagagta
     2281 aactaggaag tttatttcaa acaacctaaa atactgattt tcggctgaag ataatactgg
     2341 agtgcaaatt aatggggtta taataaatag ctgacagctt gtgttagttt ggatttttta
     2401 agagtagatg agtattaaaa ctataaggag gacgaaggtg gctaaaaatt taaaattaaa
     2461 attagctcgg gtagagcgtg atttaacaca aggtcaactg gcagaggctg tcggggtgac
     2521 acgccagact attggtttga tagaggcggg aaaatacaat cccagtctct cgctctgcca
     2581 gtctatttgc agatgtttag ggaaaaccct agaccaacta ttttgggagg aagaagatga
     2641 aaaatagatt ttattattat caattactag acgaaagaga agaacaactg atcaataaag
     2701 cgggctctga aagtttctat atctgcatcg ctttgtcgct cctatcttat atcatttcag
     2761 tattagcacc aagccttttt aattctaata tgctgctaat cgttatcatc atagggacat
     2821 tttacttttt taaccgtgcc cgttatctgg gagtgaccta ctatagtcgt tttcatttta
     2881 cgattttggg ttgttttttc ctaaccttgg ctattacggc tcttttgatg ttgcagaatt
     2941 atcaattcaa catagaaatt tatcagcaca atcctttgaa ttttaaatac ctgtctgctt
     3001 gggtcattac ttatgtcatt taccttccgt gggtctttat tggcaatctt ggtcttaaga
     3061 gctatggcga atgggctcag aaaaaatttg aacaagatat ggatgaattg gagagtggag
     3121 aatagcttgt tactcttttc tcaatccagc taaaatgtga tataatagta ctaatttatt
     3181 ggaatacatg aaagttcttg aaaattttca tgggtttcta gctaaggaag taggaaaagt
     3241 atgtatccag atgatagttt gacattgcac acggacttgt accagatcaa catgatgcag
     3301 gtttactttg accaagggat tcacaataag aaggcggtct ttgaggtgta tttccgccaa
     3361 cagcctttta agaacggcta tgcggttttt gcaggtttgg aaagaattgt gaactatctt
     3421 gaagacttgc gtttttcaga tagtgatata gcctatttgg agtcgcttgg ttatcatggg
     3481 gcgttcttgg attaccttcg caatttcaag ttggagttga ccgttcgttc tgcccaagaa
     3541 ggggatttgg tttttgctaa tgaaccgatt gtgcaggtgg aaggacctct agcccaatgt
     3601 cagttggtcg aaacggctct tttgaacatc gtcaactacc agaccttggt ggcgacgaag
     3661 gcagctcgta ttcgttcggt tatcgaagat gaacccttga tggagtttgg gacacgtcgg
     3721 gctcaagaaa tggatgcggc catctgggga acacgcgcag ctgtgattgg tggcgccaat
     3781 ggaaccagca acgtgcgtgc gggtaagctc tttgacattc ctgttttggg aacccatgcc
     3841 catgccttgg tacaggttta tggcaatgac tatgaagctt tcaaggctta cgctgcgacc
     3901 cacaaaaatt gtgtctttct tgtggatacc tatgacaccc ttcgcatcgg tgtaccagct
     3961 gccattcagg tggcgcgtga gctgggtgat cagattaact ttatgggtgt gcggattgac
     4021 tctggggata ttgcctacat ttctaagaaa gtccgtcagc aactggatga ggctggattt
     4081 acagaggcta agatttatgc ttctaatgat ctagatgaaa ataccatcct caatctcaag
     4141 atgcaaaagg ccaagattga tgtctggggc gtgggtacca agctgattac agcttatgac
     4201 cagccagctc ttggtgcagt ttacaagatt gttgcaatcg aagatgaaac tggtcagatg
     4261 cgcaatacga ttaagctgtc taataatgcg gaaaaagtct ctacgccagg taagaagcag
     4321 gtgtggcgca ttaccagtcg tgaaaaaggc aagtcagaag gcgactatat cacttatgat
     4381 ggtgtggata ttagcgacat gacagaaatc aagatgttcc atccgaccta tacatacatc
     4441 aagaagacgg ttcgtaattt tgatgccgtt cctctcttgg tggatatctt caaagaagga
     4501 atattagttt acaacttgcc tagtttgact gacattcagg attatgcccg taaggaattt
     4561 gacaagttgt gggatgagta taagcgtgtg ctcaatccgc agcactatcc agtggatttg
     4621 gcgcgtgatg tatggcaaga taagatggac ttgattgata agatgcgcaa ggaagccctt
     4681 ggtgaaggag aagaagaatg agtttgcaag aaacgattat ccaagagctg ggtgtcaaac
     4741 cagtgattga tgcccaggaa gaaatccgtc gttctattga tttcttaaaa agatatctga
     4801 aaaaacatcc cttcctaaaa acctttgtac tagggatttc tgggggacaa gactcaacct
     4861 tggcagggcg tttggcgcaa ttagctatgg aagaactgcg agctgaaacg ggagacgaca
     4921 gctacaaatt tatcgctgtc cgcctgccat acggagtgca agctgatgaa gcagatgctc
     4981 aaaaagccct agccttcatc cagccagatg tcagcttggt tgtgaatatc aaggaatcag
     5041 ctgatgccat gacagctgca gttgaagcga caggtagtcc tgtttcagac ttcaacaagg
     5101 ggaatatcaa ggcacgttgc cgtatgattg ctcagtatgc ccttgctggt tcccatagcg
     5161 gagcggtcat tggaacagac cacgccgcgg aaaatatcac aggtttcttt accaagtttg
     5221 gtgacggcgg tgcggatatt ctccctcttt accgcctcaa taaacgccaa ggaaaacagc
     5281 tcttgcagaa acttggcgca gagccagccc tttatgaaaa aatcccaacg gcagacctag
     5341 aagaagataa accaggccta gctgacgaag tcgcacttgg agtcacctac gcagagattg
     5401 acgactacct agaaggcaaa acaatcagcc cagaagctca agcgaccatt gaaaactggt
     5461 ggcacaaagg ccaacacaaa cgccacttac ccatcactgt atttgatgac ttttgggagt
     5521 aaaaacctcc attggaggtt tttaccctga gcctagaaat gagaaagcga agaaggtccg
     5581 gggacctttt tagcttcttg ccctgaaatt aaaaagcaag aaaaacctcc actggaggtt
     5641 ttcagcctct catcttgaaa taagaaagtg agagaaggtc tgggggatct tgaaccccga
     5701 gtttagaaat aagaaaatga ggcagattcg gtaactcgaa gagttcgatt tcatcgtctt
     5761 acccctgcaa cgatgactag gtttgaaaaa gcttgctaga gcgcatttca aaccaggcag
     5821 cgactgcgtc aagaaattag aagacaaact cgttttctag ctgttactga gttgagcctt
     5881 tttactacga gcatagaaat aaggaagtga ggtagcatca tgaaatctat cggtacgcaa
     5941 atattacaga cagaacgttt gattttaaga agatttgtgg agagtgatgc agaagccatg
     6001 tttcaaaatt gggcttcatc tgctgagaat ctgacctatg ttacctggga tccccatcct
     6061 gatatcgaaa tcactcgaaa ctcgattcgc aattgggttg cttcctatac taatctcaac
     6121 tattataaat gggccatttg tctaaaagaa aacccagagc aagtgatagg agatatcagc
     6181 atcgttaaga tagacgaggc tgatttaagc tgtgaaattg gctatgtgtt aggcaaggct
     6241 tactggggaa atggtatgat gacagagact ttgaaagcta tcttggactt ttgttttact
     6301 caagcaggtt ttcaaaaggt cagagcacgt tatgccagtc tcaacccagc ttcaggtcgt
     6361 gtcatggaaa aggctggaat gtcctatcta caaaccattg ttaatggtgt agagagaaaa
     6421 ggctatcttg cggatcttat ttattatggt ataagtaggg aagaatgttg aattctattt
     6481 tctgtttcta tcgaagtcaa ctatttattg taaatataat aattagcatt ccaagtttat
     6541 ttgaaacttt aaaatagcat attgattagt acaagacaga tgttctagtt ccttctttaa
     6601 tctggtttag tattagttaa aaaatcgctt taagcttgta actaagaggg agctaatcga
     6661 ctagattctc cagccgaaca ggtggtaatg tactttttat agtgtaatcc tagctgttgt
     6721 taaatttaaa atagaatcct ctatcgagtt agggaattaa attcaaccaa ttttattcat
     6781 gttttttcta tcaaattatc taatattaaa atagtctcat tctgatgaga aaactattct
     6841 atttatactg catagttgta taaggaatga tgaaagatga gtgaaaatta tcaagttgga
     6901 atgtttgtat ctaaatatat tagcatgtat ttagataaga tgtctgcaat cctttatata
     6961 tgagattgat tacgatatag ttaaagtgtt agttcaataa tcaatatatc attctaagtc
     7021 tattttatat ataaagtata ttgtttaacg tttgatagaa aagatgctat actagaagta
     7081 ggatgtaatg gcaaaatcta atactaataa gcgctatgca ttacgtaaac tcggaaagat
     7141 ttttggttct gcggtaatcg ggctaacttt atttttagga ggaatctgaa tacaagtttt
     7201 gttcatgctg ctgatgggat tcaatatgtc agagatgata ctagagataa agaagaggga
     7261 atagagtatg atgacgctga caatgggggt attattgtaa aagtagcgac taaacctaag
     7321 gtagtaacca agaaaatttc aagtacgcga attcgttatg aaaaagatga aacaaaagac
     7381 cgtagtgaaa atcctgttac aattgatgga gaggatggct atgtaactac gacaaggacc
     7441 tacgatgtta atccagagac tggtcatgtt accgaacagg ttactgttga tagaaaacaa
     7501 gccacggata cagttatcaa agttccagct aaaagcaagg ttgaagaagt tcttgttcca
     7561 tgtgctacta aatatgaagc agacaatgac ctttctgcag gacaggagca agagattact
     7621 ctaggaaaga atgggaaaac agttacaacg ataacttatg atgtagatgg aaagagtgga
     7681 caagtaactg agagtacttt aagtcaaaaa gaagactctc aaacaagagt tgttaaaaaa
     7741 ggaaccaagc cccaagttct tgtccaagaa attccaatcg aaacagaata tctcgatgac
     7801 ccaactcttg ataaaagtca agaagtagaa gaagtaggag aaattggtaa attactctta
     7861 ctacaatcta tactgtagat gaacgtgatg gaacaattga agaaactact tctcgtcaaa
     7921 ttactaaaga gatggtaaaa agacgtataa ggagagggac gagagaacct gaaaaagttg
     7981 ttgttcctga gcaatcatct attccttcgt atcctgtatc tgttacatct aaccaaggaa
     8041 cagatgtagc agtagaacca gctaaagcag ttgctccaac aacagactgg aaacaagaaa
     8101 atggtatgtg gtatttttat aatactgatg gttccatggc aacaggttgg gtacaagtta
     8161 atggttcatg gtactacctc aacagcaacg gttctatgaa agtcaatcaa tggttccaag
     8221 ttggtggtaa atggtattat gtaaatgcat cgggtgagtt agcggtcaat acaagtatag
     8281 atggctatag agtcaatgat aatggtgaat gggtgcgtta acagattcct ccctgcgaca
     8341 aaattgataa tagtatgtct gaatatataa ttatatataa ttatatataa aaagctatag
     8401 ataaggtcat cttatagcga ctataaaaaa gtgaacaagc ctagtcttct gtacattaga
     8461 attcagattt gttcactttt tgtttaatac tcaatgaaaa tcaaagagca aactagaaaa
     8521 ctagtcgcag gttgctcaaa gtactgcttt gaggttgtag atagaactga cgaagtcagt
     8581 cacatatata atccaaggtg acgttgacgc agtttgaaga gatttttgaa gagtataagt
     8641 ctttgatttt gagactaagg agagtcaggg tcaaagtagt gaatgattct gaaataactc
     8701 ataatagtct cttgagagga cagataagag taacaaatat ggtgctactc atgtgtcttg
     8761 cattttgatt tacaaacagc gaagaaaata taggaagctg aaataaaata tagatgaatc
     8821 aatttggaga gtcaacgcaa tctatggcaa tattttcgaa gctaggggga agcattttaa
     8881 tttcaatgta ctaggcgtct tctacagcta gctagagttt caattgtaga tgttctttga
     8941 aataatatct tagcgattta ttggtattag aagatttaga gaaattcttt ctcaataaat
     9001 aaacatggag tcaccaaaac tgaagaagcg gtagtgttct tggatggcat ggtggtaggc
     9061 atctaagact aattcacggc ctgcaaaggc agaaaccaac atgaccagag ttgattttgg
     9121 cagatggaag ttggttgaga aggcatccac gaccttccac tcatacccag gtttgataaa
     9181 gatattggtc caaccagaat ctgcttggat ttgcccatca aacttggaac caatagtttc
     9241 caaggtgcgg atagaagtgg ttccgacagc gatgacgcga ccaccatttt ctttgacaga
     9301 gcgaagggtg gcagcagctt cctcagaaag ttgatagaac tctgaatgca tttcgtgttc
     9361 gtccagatta tccacagaaa caggtctaaa ggttccgagt ccgacatgga gagtcagata
     9421 gactagatga acacccttag cttggatttc tgccagcagt tctttggtga agtgaagacc
     9481 agcagtcggt gctgcagcag agccgctttc cttggcgtag acggtttgat aacgttcacg
     9541 gtcatctaat ttttcgtgga tataaggtgg cagaggcatt tctcccagac tttccaagac
     9601 ttctaggaaa attccttggt attcaaagcg gacaatgcgt cccccgtggg tcaattcttc
     9661 tgtaacgaca gcgctgaggc ggccatcacc aaagctgata cgagtaccga ccttgaggcg
     9721 tttggcaggt ttagccagaa cttcccactc gtctccacta gtgttcttaa ggaggagaag
     9781 ttccacatga cctcctgtct ccactttttg accatagagg cgggcaggga gaactcgggt
     9841 gtcgttcatg acaagggcat caccaggttc cagcatatta ataatagagt ggaagtgttt
     9901 atcttgcatt tctcctgtct cacggttgac gatgaggagt ttggaggcat cacgtttttc
     9961 aaggggcgtt tgggcaatca attcctctgg caagtggaaa tcaaaatcag ctgtattcat
    10021 atatctgttc attctttcta agttctaatc ctatccatta taacacgttt caagtttgga
    10081 cgctcttttt ttagaaatta aatcgttttc acttgacaaa aattggtcta taccatataa
    10141 taaatataga cagaaatgga ggatgaaaag atgaaagtta ttaaagttga aaaccaagtt
    10201 caaggtggaa aagttgcttt tgagattttg aaggaaaaat tggctaatgg agctcaaact
    10261 ctaggacttg cgacaggaag tagcccactt gaattttaca aggaaattgt tgagagtgac
    10321 cttgattttt caaatctaac cagtgtcaac cttgatgagt atgtgggcct tgatggggac
    10381 aatccacagt cttatcgtta cttcatgcaa gaaaacttgt tcaaccaaaa accatttaaa
    10441 gaaagtttct tgcctcgtgg tgttaaggac aatgctgaag ctgaagttga acgctacaac
    10501 caaattttgg ctgaccatcc agttgacctc caaatcttgg gaatcggtcg caatgggcat
    10561 atcggcttta atgagcctgg tactccattt gacagtcaaa cgcatctagt agaacttgac
    10621 cagtctacta tcgaagccaa tgcacgcttc tttgccaaga ttgaagacgt cccaacccaa
    10681 gctatttcaa tggggattaa aaacattttg gatgccaagt ctattattct ttttgcttac
    10741 ggtgagtcga aagcagaagc cattgccgga acagtatctg gcccagtgac tgagaaccta
    10801 cctgcaagta gcctccaaaa tcaccctgat gtaaccatca tcgcagatgc tgaagcgctt
    10861 agcttactcg aaaaataaaa aagcaatgtt ctattgaacg ctttcaactc ttgtataaat
    10921 ggaagaaaaa atcaaaatta aacagcattt ttgcttgaca attattcctt ttacgtgtag
    10981 aatgaaatag atatcgaact tgaagggagt gaaaaaaatg tctaaaacag tagtacgtaa
    11041 gaatgaatct cttgacgacg cacttcgccg tttcaaacgt gcggttacta aagctggtac
    11101 tcttcaagaa acacgcaaac gtgaattcta tgaaaaacct tctgtaaaac gtaaacgtaa
    11161 atcagaagta gctcgtaaac gtaaaaaatt ctaattggaa tgaaaggcta gagaaatcta
    11221 gtcctttttc ttttaaataa atactccaaa gcctgcaaaa atctgaaact tcctcctaca
    11281 atttgatata atagagagaa gaactcattt gaaggaggaa atgatgtcgg ttttagtaaa
    11341 agaagtgatt gaaaagctta gactagatat tgtctatggt gaaccagagt tgcttgaaaa
    11401 ggaaatcaat acagcggata ttacgcgacc tggtcttgaa atgacaggct attttgacta
    11461 ctacacacca gagcggattc aacttttggg gatgaaggag tggtcttatc tgatcagcat
    11521 gccttccaac agccgttatg aagttttgaa aaaaatgttt ctatctgaga caccagcagt
    11581 cattgttgcc cgtggtttgg tggttccaga ggagatgtta aaggctgcta gagaatgtaa
    11641 gattgctatt ttaaccagcc gtgcagctac cagtcgttta tctggagagt tatctagcta
    11701 tctggattct cgtttggcag aacgtaccag tgtgcacggt gtcttgatgg atatttatgg
    11761 gatgggcgtc ttgattcagg gagatagtgg aattggtaag agcgagacag gtcttgagct
    11821 tgtcaaacgt ggtcaccgtt tggtagcaga tgaccgtgtc gatatctttg ccaaggatga
    11881 gattactctc tggggtgaac cagctgaaat tttgaaacac ttgattgaaa ttcgtggggt
    11941 tggtattatc gatgttatga gtctctacgg tgcgagtgct gtcaaggatt cttcacaggt
    12001 tcagcttgct gtctatttgg aaaattacga tacgcataag acctttgatc gtcttggaaa
    12061 caatgcagag gaacttgaag tttctggcgt agccattcct cgtattcgta ttccagttaa
    12121 aacaggtcgt aatatctctg ttgtgattga ggcagctgcc atgaattatc gtgccaagga
    12181 aatgggcttt gatgcgaccc gtttgttcga cgaacgactg acaagtctca tcgctcgaaa
    12241 tgaggtgcaa aatgcttgat ccaattgcta ttcaactagg acccctagcc attcgttggt
    12301 atgccttatg tattgtgaca ggcttgattc ttgcggttta tttgaccatg aaagaagcac
    12361 ctagaaagaa gatcatacca gacgatattt tagattttat cttagtagcc tttcccttgg
    12421 ctattttagg agctcgtctc tactatgtta ttttccgatt tgattactat agtcagaatt
    12481 taggagagat ttttgccatt tggaatggtg gtttggccat ttacggtggt ttgataactg
    12541 gggctcttgt gctctatatc tttgctgacc gtaaactcat caatacttgg gattttctag
    12601 atattgcggc gcctagcgtt atgattgctc aaagtttggg gcgttggggt aatttcttta
    12661 accaagaagc ttatggtgca acagtggata atctggatta tctacctggc tttatccgtg
    12721 accagatgta tattgagggg agctaccgtc aaccgacttt cctttatgag tctctatgga
    12781 atctgcttgg ctttgccttg attctgattt ttagacggaa atggaagagt ctcagacgag
    12841 gtcatatcac ggccttttac ttgatttggt atggtttcgg tcgtatggtc atcgaaggta
    12901 tgcgaacaga tagtctcatg ttcttcggcc ttcgagtgtc ccaatggctg tcagttgtcc
    12961 ttatcggtct cggtataatg atcgttattt atcaaaatcg aaagaaggcc ccttactata
    13021 ttacagagga ggaaaactaa atgttagaag ttgcatatat tcttgttgcc ctagctttga
    13081 ttgtcttttt ggtctatctg atcattactg tacaaaagct tggtcgtgtc atcgatgaaa
    13141 cagaaaagac gattaaaacc ttgacttcag atgtggatgt gaccttgcat cacaccaatg
    13201 agttgttggc taaggtcaat gtcttggcag atgatatcaa tgtcaaggtg gctacgattg
    13261 atccactctt cagtgctgtt gcagatttat ctctatctgt ttcagacctc aatgaccatg
    13321 cgcgtgtctt gagcaagaaa gcttcatcag ctggttcaaa aacactcaag actggtgcaa
    13381 gtctgtcagc tcttcgtctt gcaagtaaat ttttcaaaaa ataaaaaagg agaatcctta
    13441 tgggtaaatt atcctcaatc cttttaggaa ccgtttcagg tgcagctctt gccttgtttt
    13501 taacaagtga taagggcaaa caagtttgca gtcaggctca agattttcta gatgatttga
    13561 gagaagatcc ggagtatgcc aaggagcaag tctgtgaaaa actgacagaa gttaaggagc
    13621 aggctacaga ttttgttctg aaaacaaaag aacaggttga gtcaggtgaa atcactgtgg
    13681 acagtatact tgctcaagct aaatcctatg cttttcaagc gacagaagca tcaaaaaatc
    13741 aattaaataa tctcaaggag caatggcaag aaaaagccga agctcttgat gactcagaag
    13801 agattgtgat tgatataaca gaagaataaa ccatcaccat ctccggacgg actatgtatc
    13861 tggggatggt gatttttatc tggaatctag tctttgtggt ataataatta ctatgcagaa
    13921 aaaaccaacg tcagcctatg tgcacatccc attttgtacc cagatttgtt attattgtga
    13981 tttttcaaag gtcttcatca aaaatcagcc agtcgacagc tatttagagc atctgctgga
    14041 agagtttcgt tcttatgata ttgaaaagtt gtcaaccctt tatatcggtg gtggaacacc
    14101 gacagccctg tcggctccgc aactggaggt tttactgaat ggcttgacta aaaacttgga
    14161 cttgtctgtc ttagaagagt tgaccattga agccaatcca ggcgatttgg atgcggataa
    14221 gatagctgtt ttgaaaaatt cggctgtcaa tcgtgtttcg ctaggtgtcc agacctttga
    14281 tgataagatg ttgaaaaaga ttgggcgcag tcatttggag aaggatattt atgaaaatat
    14341 cgatcgcctg aaactggctg gttttgacaa catctccatt gatttgattt atgctctgcc
    14401 tggtcagacc atggagcaag taaaggaaaa tgtggctaaa gccattggac tggatattcc
    14461 ccacatgagt ttgtatagtt tgattttaga aaaccatacg gtctttatga atcggatgcg
    14521 acgtgggaaa ttgcctctgc ctaaggagga actagaagcg gagatgtttg agtacatcat
    14581 tgcagagctg gagcgagcgg gttttgagca ttatgagatt tctaatttct ccaaacccgg
    14641 ctttgaaagt cgtcacaatc tcatgtactg ggacaatgct gaatactatg gtattggtgc
    14701 aggggcatct ggttatgtca atggagtacg ctataaaaat catggtccca ttcgtcatta
    14761 tctcagtgcg gttgaggaag gcaatgcttg tattacagaa gatcacctga gtcaaaagga
    14821 gcaaatggaa gaagaaatgt tcttgggact ccgcaagaaa tccggggttt ccatggcgcg
    14881 atttgaggaa aaatttggac agtcttttgc tggactttat ggagaaattg tcagagattt
    14941 ggttcaacaa ggcctcatgc agattgaggg tgaccacgtt cgcatgacaa agagaggtct
    15001 ctttttggga gatactgtag cggaacgatt tattttggag taggatcatg ggcttaactt
    15061 atcaaatgaa aatgaaaatt ccttttgata tggctgatat gaacggtcat atcaaacttc
    15121 cagatgtaat tttgctgtca ctgcaagttt cagggatgca gtcgattgaa ctgggagtta
    15181 gtgataaggc cattttggaa gactataatc tggtctggat tatcgtagaa tatgatattg
    15241 aggtggttcg tttgcctcgt tttgcggaag aaattaccat cgaaacggaa gccttgagct
    15301 acaatcgact tttttgctac cgtcgcttta ctatttataa tgaagcgggg caggagctca
    15361 tccatatgat ggcgaccttt gttctcatgg accgcgacag tcgaaaagtc catgctgttg
    15421 aacctgagat tgtggctcct taccagtctg attttgataa aaaacttatc cgtggaccga
    15481 agtatgagtc cttggaagag tcgatcagca aggattacca tgttcgtttt tacgacttgg
    15541 atatgaatgg tcatgtcaat aacagtaaat acttagactg gatttttgag gtcatgggag
    15601 cggatttttt gacccactat attcccaaga aaatcaacct caagtatgtc aaggaagttc
    15661 gaccaggtgg ggtgattaca tcggctgttg aacggactgg actggaaagc aagcatgaaa
    15721 ttacaagtga tggggttatc aatgcccaag caatcattac ttggcaagaa ataaaaaagg
    15781 cttagggaga aatagatgaa atataaaggc tatttaattg atttagacgg aaccatttat
    15841 aaggggaaag accaaatccc tgctggagag acttttgttc atgaattgca aaagcgggac
    15901 attccctatc tctttgtaac caacaataca acccgcactc cagagagtgt taaggagatg
    15961 ttggctcaga attttaatat cgatacgccc ctatcgactg tctacacagc gactttggca
    16021 accatcgact atatgaatga tttggggctt gaaaagaccg tctatgttgt cggagaagca
    16081 ggactcaagg aagccatcaa ggcggctggt tatgtggaag acaaggaaaa acctgcctac
    16141 gtggtagttg gtctggattg gcaagtcgac tatgaaaaat ttgccacagc aactcttgct
    16201 attcaaaagg gtgcccattt tatcggaacc aatcctgacc tcaacatccc gacggaacgt
    16261 ggtcttttgc caggtgctgg ctcactgatt acgctgcttg aagtagcgac acgagtgaag
    16321 ccggtttata tcggaaaacc caatgccatc attatggaca aggcagttga gcacttaggt
    16381 ttggaacgtg aagagttaat catggttggg gacaattatt tgactgatat ccgggctggg
    16441 attgacaatg gcattccaac gctcttggtg acgacaggtt ttaccaaggc agaagaagta
    16501 gctggcctgc caatcgcacc gacacatgtg gtttctagcc ttgcggagtg ggattttgat
    16561 gaaaactaaa ttgacctttt ggggctctat gctctttctc ctctccctct caatccttct
    16621 gaccatttat ctggcttgga ttttctatcc tatggagatt cagtggctaa acttaacgaa
    16681 tcgagtctat ctaaaaccag aaaccattca atacaatttt catatcttga tgaattatct
    16741 gaccaatcct tttagtcagg tcttacagat gcctgatttt cgttcgtcag cagctggttt
    16801 gcaccatttc gcagtggtca agaatctctt tcatttggtt cagctagtag ctctagtgac
    16861 actgccaagt ttctatgtct ttgtcaatag gattgtgaaa aaggactttt tgtctcttta
    16921 tcgaaaaagt ctcctggctc tagtagtctt acctgtgatg attggacttg ggggagtttt
    16981 gattggtttt gaccaattct ttactctttt ccatcaaatt ctctttgtgg gagatgatac
    17041 ctggcttttt gatccagcca aggatcctgt tattatgatt ttgccagaga ccttctttct
    17101 tcatgccttc ctcctctttt ttgccctcta tgaaagcttc tttggctatc tgtgtctgaa
    17161 aagtcgtagg aagtgaaagg aaagatgttt ttaatttaca tatataaata tatatatcgt
    17221 ttaaaaccat cgttaagagt gaattaacca aatccaattg tgtcaatcgt tcgcaaacag
    17281 cctatttttc ctgaaaaaat aggctttttt tctaaaaatc cctagtcaag cgccttattt
    17341 ttttgtataa tagaagtagc aaagtataga taagaagaga aaagcatgat tacactattt
    17401 ctatcaccga gctgtacatc atgtcgtaag gctaagacct ggttagaaaa acataaggtt
    17461 ccctttgtgg aacacaatat tatgaccagt cctttaacaa gaaaagaatt gcaacacatc
    17521 ctttccttga ccgaaaatgg tactgatgac atcatttcaa ctcgttcaaa aatttttcaa
    17581 aaattgaata ttgatgtaga gagtatttcg gtatcggaat tgcttcattt gattgagcag
    17641 tatcctagtc ttttgcgtcg tccaattatt attgatgcca agcgtatgca aatcggtttt
    17701 aatgaagatg agattcgtgc ttttctccct cgtagttatc gtaagcaaga actaaaagaa
    17761 gcaagaatga gagctggtat tagttaatga acaaacagta tagttaccca ctagatttgt
    17821 cgtggagcac tgaagaactt gcttcagtgc tttctttttt taatgatgtt gaagctgcct
    17881 atgaaggcaa agtagaggct aaaaagttac tggactccta taagggattc aaagcggtcg
    17941 tgccaagcaa gagcgaagag aaacgtttgg gacgagaatt tgaaacggtt agtggctatt
    18001 cactttaccg agcagttcag gctgccaaag aaaaagggaa aggaaagatt tctcttgaaa
    18061 agtaaatttg aatttgctaa agagcttgtt aagaaagcag gtcagtacat tcttgaccat
    18121 atgcaggaag acttgcgtgt tgaaaccaag tcctctccaa cagatttggt gaccagactg
    18181 gacaaagaag ttcaggaact cttggttggt gagattttgt cccgttatcc tgaggataag
    18241 atttgtgctg aagaaggttg tctgcgagcc tcggttcaag agggcaaggt ttgggtcatt
    18301 gatcccattg atggtaccaa taattttgta gcccagcagg aagattttgc tgttatgatg
    18361 gcttattttg aaaatggtca gggacagttt ggtctgattt atgatgtggt caaaggggat
    18421 tgttaccacg gtggtgggga atttccagtt tgtctaaatg ataggcccct agcttctttt
    18481 aaaactaaac cacttggaga ttttctcatt gcagggaata gtggtatgct ggaaaccaat
    18541 gaatgggggc tggctgattt gagccgagcg gttcttggtg ttcgtgtcta tggaagtgcg
    18601 gccattagtt ttgccaagat tttgtcaggg cgtttgttga cctatctcac ctatctgcaa
    18661 ccatgggatt atgccgcagc tagtatttta ggggaaagtc tgggctatcg ggttgtgacc
    18721 ctttttggtg aagctcctga ttttcaaacc agacagccgg tcatgatggt gcctcttgag
    18781 atgcaggagg aaattcagtc ctatatttac gaaaggaaaa gaacttaaat gaaatttcca
    18841 gaaggatttg ttgaaaaata taaagaaata ctaggagatg aggcaagaga ttttcttgcc
    18901 tcttttgagg aggaagcggt ttcggccttt cgggtcaatc ccttaaaaga agagcaactt
    18961 tccttttctg atgccattac tcaaacccct tggggccact atgggaaggt ttcagggaaa
    19021 tcgcctgagc atgctacagg tttagtttat tcgcaagaac ctgctgctca aatggtggct
    19081 caagtagccc aacccagtcc tggtatgaag gtcttggact tggctgctgc accggggggc
    19141 aaatcaactc aactggcagc ctatctagca ggggaggggc tacttgtttc caatgaaatt
    19201 tcaagcaaac gggctaagat tttggttgaa aacatggagc gttttggagc gacaaatgtc
    19261 gtggtgacca atgaatctgc cgaccgcttg gtcaaggtct ttaaaggcta ttttgactta
    19321 atcgttcttg atgccccttg ctctggtgaa ggaatgtttc gtaagcaacc tgatgctatg
    19381 gactattgga gcttagatta tccgagtcaa tgtgctagct tgcaaagaga aattctggag
    19441 gatgcagtga ccatgttagc tgaaggtggt cgtctggttt attcgacctg tacctgggca
    19501 cccgaggaaa acgaagagat tgtcaattgg ttgctggaag agtatgattt tgacttgttg
    19561 ccagtagagc atatcaatgg aatggtagct ggtattgacc tgccagaaac ggctcggatg
    19621 taccctcatc agtttaaggg agagggtcag tttgttgccc atctacagtt taagggtaac
    19681 aatccagctc ctaaatttaa ggcaagtaag agtaacctca gccgtgaaca agttgccttg
    19741 tggcaggaat ttgcccaaaa ccatttgaag gtcaatctac cgggtatctt gcagattttt
    19801 ggagaccagc tttatctctt gccagaactt ttgccagatt tagggaagct caagattgct
    19861 cgcaatggac tgcatctggg tacttttaag aagaaacgct ttgagcccag tttcgctctt
    19921 ggtttagcct tgaaaccgag tcaggtcgaa cagtcggttg aaattggcca agaagagttt
    19981 gtcaagtatg cggctggaga aaccgttcag ctggcagaaa gtctgccaaa tggttggtac
    20041 caagttttgg ttaaaggcaa tggcttgggc tttgcaaagg ttactggaaa tgttttgaaa
    20101 aattattttc caaaaggcct cagattcaaa tgaaaatgct agtcaaatat cttattctat
    20161 gttatagtga aagtatggat ttttttagta ggatgattct tgatatcttg attgccatca
    20221 catgtagttt tactactcga tgaaaatcaa agagcaaact aggaagctag ccatagatag
    20281 ctcaaaacac tgctttgaag agattttcaa agagtacaag aagtttagtt attagcgttc
    20341 ttaccgcttg taaactagat ttctcataaa atagaatctt ttccttttag ttgtaaacta
    20401 gtctgggaga gtagagagat ttgagatacc tttctagctt ttggattatc atctaaggag
    20461 agtaatttcc cttgcattaa aaaggggaaa aagagacacg aaatgactat aatgggtgac
    20521 aatgggggaa gggatagaca agagatttta tccacatatg aaaaaaggag gttaggaaag
    20581 agtaatatag cctatattat ataaataatc aattgcgcag aaatttggta agaattcatg
    20641 cgtcaactca taaagaacta cttaaaaaat tcacagtatt cataattatt ttcgaggaga
    20701 aaaacagtga aaaaaagaaa aaagcttgct ctgtctctta tcgctttttg gctgacggct
    20761 tgtttagtag gctgtgctag ctggattgat cgtggagaat ccataacggc tgttggctca
    20821 actgccttgc aacccttggt tgaagtagcg gcagatgaat ttggcacgat ccatgttgga
    20881 aaaacggtca atgtccaagg gggaggttct ggtacaggct tgtcccaggt tcagtctggg
    20941 gcagttgata taggaaactc agatgtattt gctgaggaaa aagacggaat tgatgcttct
    21001 gctcttgttg accacaaggt cgcggtagct ggcttggctc tgattgtcaa taaggaggtt
    21061 gatgttgata acctaacgac agagcaactt cgtcaaatct tcataggtga ggtaaccaat
    21121 tggaaagagg ttggtggtaa ggacttaccc atctctgtta tcaatcgggc agccggctct
    21181 ggctctcgtg ctacctttga tactgtcatt atggaaggtc agtctgccat gcaaagtcag
    21241 gagcaggatt caaatggagc ggtaaaatca atcgtatcaa aaagtccagg agctatctct
    21301 tatttatctc ttacctatat agatgattcg gtcaaaagca tgaagttgaa tggctatgac
    21361 ttaagtccag aaaatataag tagcaataat tggcccttgt ggtcttatga gcatatgtat
    21421 acattggggc agcccaatga gttagctgca gaatttctca attttgttct ctcggatgag
    21481 acccaagaag ggattgtcaa aggattgaag tatattccga ttaaggaaat gaaggttgaa
    21541 aaagatgctg ccggaactgt gacagtgttg gaagggagac aataatgaat caagaagaat
    21601 tagctaagaa aatgttgctt ccatcaaaga attctcgtct ggagaaatta ggaaaaggtt
    21661 tgacctttgc ctgtctttct ttgatagtca tccttgtggc catgattttg gttttcgtag
    21721 cgcaaaaagg cttgtcgacc ttctttgtca atggtgtgaa tatctttgac tttcttttgg
    21781 gaggaacttg gaatccttct agtaaagaat ttggtgccct tcctatgatt ttgggttcct
    21841 ttatcgttac cattctctca gcccttatcg caacaccctt tgctattggt gcagcagttt
    21901 ttatgaccga agtatcacca aaaggggcga agattttgca accagctatt gaactcctgg
    21961 ttgggattcc ttcagtagtg tacggattta ttggcttgca agtcgtcgtt ccctttgttc
    22021 gcagtgtctt tggtgggact ggttttggga ttttgtcagg gatttccgtc ctctttgtca
    22081 tgattttgcc gaccgtaacc tttatgacaa cggatagctt gcgtgcggtt cctcgttatt
    22141 atcgtgaggc aagtttcgct atgggagcca ctcgctggca gactatctgg cgtgtgatct
    22201 tgaaggcggc ccgttctggt attttcactg cagtggtctt tgggatggcg cgtgcctttg
    22261 gtgaggcttt agctatccag atggttgtcg gaaactcagc tgttatccca acttccttga
    22321 ccacaccagc tgcaacttta acttctatat taactatggg aattgggaac actgtcatgg
    22381 gaactgtaaa taataatgtt ctctggtcac tggccttggt actgctcttg atgagtttag
    22441 tttttaacag tgtgattaaa ttgattacga aagaaagagg aaagaaaaat tatgcgcgct
    22501 aagaaattag ataaacttgc aacagctgtc ctctatacga ttgctagcat cattgtgaca
    22561 atcttggctt ccttgattct ctatatcttg gttcggggct tgccccatat ctcttggtct
    22621 ttcttgactg gaaggtcttc tgcttttcaa gcaggtggtg ggattggcat tcagctttac
    22681 aattcctttt tcctattggt cattaccttg attatttctg tacctctttc tatgggagct
    22741 gggatttact tggctgaata tgctaaaaaa ggtcctgtta ccaactttgt gcggacttgt
    22801 attgaaatct tgtcctcttt accatcagtg gtggtgggtc tctttggtta cttgatcttt
    22861 gtagtccagt ttgagtatgg attttcaatc atttcaggtg ccttggcctt gacagtcttt
    22921 aacttgcctc agatgacgcg taatgtagag gatagtttga aacacgttca ccatacccaa
    22981 cgtgaggctg gtctggctct tgggatttct cgctgggaga cagtggttca tgttgttatt
    23041 ccagaagcgc ttccaggtat tgtaacgggt gtcgtcttgg catctggtcg tatctttggc
    23101 gaagctgcag ctctgatcta tacagcaggg caatcggcgc cagctcttga ctggtctaac
    23161 tggaatatcc tcagtgtgac tagccccatc tctatcttcc gtcaagcaga aaccttggct
    23221 gtccatatct ggaaagtcaa tagtgaaggc actattccag atggaaccat tgtatcagca
    23281 ggttctgccg ctgtgctcct gatctttatc ctgattttta actttggagc tcgtaagttc
    23341 ggaagctatc tacacaagaa attaaccgct gcctaaagga gaagccatgt caacatataa
    23401 ctgggatgag aagcatatcc ttacctttcc tgaagaaaaa gtagcccttt ctactaagga
    23461 tgtccatgtt tactatggta aaaatgaatc cattaagggg attgatatgc aatttgaaag
    23521 aaataaaatt acagctttga ttggtccgtc gggatcgggg aaatctacct acttacgcag
    23581 tctcaatcgc atgaatgata ctattgatat tgctaaagta actgggcaga ttctctatcg
    23641 tggaattgat gtcaaccgtc cagaaatcaa cgtttatgaa atgcgtaaac acattggaat
    23701 ggtttttcaa cgccccaatc catttgctaa gtcaatttac cgtaatatta cctttgcgca
    23761 tgaacgtgct ggagttaagg ataagcaagt cctagatgaa atcgtagaaa cctcccttcg
    23821 tcaggctgcc ctctgggatc aggttaaaga cgatctccac aagtcagcct tgaccttatc
    23881 aggtggtcag caacaacgtc tctgtatcgc tcgtgccatc tctgttaagc cagatatcct
    23941 cttaatggat gagccagcct cagccttgga tccgattgcg accatgcaac tagaagagac
    24001 catgtttgag ctcaagaaaa actttaccat catcattgta acgcataata tgcagcaggc
    24061 tgctcgtgca agtgactata caggcttctt ttacttgggt gatttgattg agtatgacaa
    24121 gactgcaact attttccaaa atgccaagct acagtccacc aatgactatg tatctggtca
    24181 ctttggttag aaaggaaacc gtatgacaga tgcgatttta caggtatcag acctgtccgt
    24241 atattataat aaaaagaagg ctttgaatag tgtttcccta tctttccaac ctaaggaaat
    24301 tacagccttg attggtccat ctggatcagg gaagtcaacc ctcctcaagt ctctcaaccg
    24361 catgggagat ctcaatccag aggtgaccac aactggatcc gtggtgtaca atggtcacaa
    24421 catctacagt ccgcgtacag atacggttga attacgtaag gaaatcggaa tggttttcca
    24481 acaacctaat cctttcccta tgactatcta tgagaatgtt gtctacgggc ttcgtatcaa
    24541 tggaattaag gataagcagg ttctggatga agccgtagaa aaagccttgc aaggtgcctc
    24601 tatctgggat gaggtcaagg atcgtctata tgattcagct attggattgt caggtggtca
    24661 acagcagcgt gtctgcgtgg cccgtgtctt ggcaactagt cctaaaatca tcctcttgga
    24721 tgagccaact tcggctttgg atccgatttc agctggtaaa attgaggaaa ccttgtatgg
    24781 tctaaaagac aagtacacca tgcttctggt aacccgttcc atgcagcaag cttcacgtat
    24841 ctctgataag acaggatttt tcctagatgg agatttgatt gaatttaatg ataccaagca
    24901 gatgttcctt gatccccaac acaaggaaac ggaagactat attacaggaa aatttggata
    24961 aggagatgaa agatgttacg atctcaattt gaagaagatt tagagaaatt acataaccag
    25021 ttctacgcta tgggacaaga agtgctctca caaatcaatc gtacggtacg tgcttttgtc
    25081 acgcatgacc gtgacctggc aaaagaggtc atcgaagatg atgcagaagt aaatgaatac
    25141 gaagtgaaac tggaaaagaa atcatttgaa atgatcgcac tccaacaacc agtctctcaa
    25201 gatttgcgta cagtcttgac tgtccttaag gctgtatcag atgtggagcg tatgggggat
    25261 cacgctgtag ccattgccca ggcaaccatc cgtatgaagg gggaagaacg catcccagct
    25321 gtagaggaag aaattaaaaa aatgggacgt gaagttaaaa gcgttgtgga agcagcactt
    25381 gatctttatc ttaatggttc tgttgacgac gcataccggg tggcctccat ggatgagcaa
    25441 attaaccact attttgaaac tatccgtgac cttgcgactg aagagattaa gaaaaatcca
    25501 gaagccattg ttacgggtcg tgattatttc caagttattt cctacttgga gcgtatcggt
    25561 gactatgcta aaaatatctg tgaatgggtt gtctactttg aaacaggtaa gattgtcgaa
    25621 ctataaaata gtttaattct gtataaaaag gagcttgaaa tcaactgatg gctccttttt
    25681 ttgaacgaaa aaatattttt gagataaaaa tttaaaaaac acttgacaag taacttggat
    25741 atcgttataa ttatacaaaa ttaacagaaa ggttgtttat ttatgaaatc aaaaaaaatg
    25801 gatatttgtt ttatgtaatt ttcttgcaag tttcttctta gtagcttgcc agtcaggttc
    25861 taatggttct cagtcagctg ttgaggccat taagcaaaag gggaaattag ttgtggcaac
    25921 cagtcctgac tatgcaccct ttgaatttca atcattggtt gatggaaaga accaggtagt
    25981 cggtgcagac atcgacatgg ctcaggctat cgctgatgaa cttggggtta agttggaaat
    26041 cttaagcatg agttttgaca atgttttgac cagtcttcaa actggtaagg ctgacctagc
    26101 agttgcagga attagtgcta ctgacgagag aaaagaagtc tttgattttt caatcccata
    26161 ctatgaaaac aagattagtt tcttggttcg taaggctgat gtggaaaaat acaaggattt
    26221 aactagccta gaaagtgcta atattgcagc ccaaaaaggg actgttccag aatcaatggt
    26281 caaggaacaa ttgccaaaag ctcaattaac ttccctaact aatatgggtg aagcagtcaa
    26341 tgaattgcag gctggaaaaa tagatgctgt tcatatggat gagcctgttg cacttagtta
    26401 tgctgctaaa aacgctggct tagctgtcgc aactgtcagc ttgaagatga aggacggcga
    26461 cgccaatgcc gttgctctta gaaaaaatag tgatgatttg aaagaagtgg tagacaaggt
    26521 catccaaaaa cttaaggatg aaggaactta ccaaagctat cttgaaaaag cagcaagtct
    26581 aactgaagtt gaagaataag aaaaagcaga gttggatgtc attccaattc tgcttttaaa
    26641 tagtttaaaa cattttccag attgtctagg gacaccttgg caaattgata aggacaggtg
    26701 ctcaaatagg cctcttcaaa gaagtccaag cccagttgac tgtgtttgag actggcgatt
    26761 ttaggatact gtctcaggtc tagcaataaa ccatcgtgta ggggataatg aggaagggga
    26821 caaggtgttt tagttatcct ttcctcgatt tgtttttggt aagattcatg attggtcaag
    26881 cgagccaaac gatttttttc aaacaattga ctgtcaatat agagtgacag ggccttttgc
    26941 ataatgaggt tgctgtcgta gtctaggata tttttgtagg ccacaaatgc ttctttgata
    27001 gcatttggaa gaatgagtgc tgtaatacga agggcaggaa aaaggctggt cgagaaggac
    27061 ttgatataaa tgacatgctc ctctgtatca agatagtgga aggtttggcc cttcttggag
    27121 tccaaatcac ccagataatc gtcctctacg atatagacat catacttggc agctaagtta
    27181 agaatagatc gtttgtcttg ctcagaatag gaatgtccca gaggatagtg aaatcgggga
    27241 atggtgtaga aaaatttaat ttttcctgtt ttgaagtggc cttccagctc ctccaagtca
    27301 atcccatcaa tgcctcgttc aatcgtttga tagtccagcc cctgtgcaat caagaggcga
    27361 ttcatccgat ggtaggttgg ctgttccacc aagatttcct tggcttgtct aggaaaggat
    27421 atttgagaga ggataaataa ggcttgttgg gttccagaag tcagtactag ttggttagcc
    27481 ttgcagtaga gagcttgctc aaagaggagt ttgtgaatgg actgtcttag gtcttctaat
    27541 ccttcttgat tgtcatagta gttgaagagg tagttttctc ggccaatcaa ggtttcattg
    27601 acacagagtc ggaaatcgtc ataggcactg gcatgttcgt cggtaacctc gatttctagg
    27661 tcttgatgtt gcccttgttc taatacatag tagccactct gaggcttggc atagaggtat
    27721 tgttcgtgcc gtaattccag cagggctcgt tgaatggtgt ccttgctgca gtgaaagtca
    27781 aggctcagtt gacggataga aggcaggcga ctacccgtcg gaaatcgtcc agactcgata
    27841 ccatttttca gataggaaac gacctctttg tacttgcttt gtttcttcat tccagacctc
    27901 ccttgatttt gttacattgt accctttttt ttccttcttg gcaatgtcta gaacgtttct
    27961 ctcgttcaca tctaggggca aatgtggtat acttaggagt aagatttata gaataacaga
    28021 caagaaagtg aatggataag aacgtgcagg aaccagtgaa attatttcaa tacaatactc
    28081 ttggagcctt gatggctggc ctttatggtg gtactatgac agtaggagaa ttgctagaac
    28141 atggtgacct tggtttggga accttggatt ccattgatgg ggaattgatt gtcttggatg
    28201 gcaaggctta tcaggccaaa gggtcaggag accagccaga aattgtggaa gtgtcaccag
    28261 atgcccttat tccttacgct gcagtggtac cgcatcaggc agaggtcatt ttccgccagc
    28321 gctttgagat gacagacaag gaattgaaag aacgaattga gtcttattat gatggggaaa
    28381 atcttttccg ctctatcaag attcgggggg aatttttaca tatgcatgtg cgtatgattc
    28441 ctaagtcaac acccgatacc aagtttgctg atgtcgcaac ccatcaaccg gaatatagtc
    28501 gtgacaatgt tgcggggact attgttggtt tctggacgcc tgagattttc catggggtca
    28561 gtgtggcagg ctaccatctg cacttcatat cagatgattt gaccttcggt ggacatgtca
    28621 tggattttgt catcaaggaa ggcattatcg aggtgggagc agttgaccag ttggaccaac
    28681 gtttcccagt ccaagaccgt caatacttgt ttgctaagtt caatgttgac gagatgaaaa
    28741 aagatattga aaaggcagaa taggagaaga aaatgaccat tcatatcatt attaccatgt
    28801 tgctgttgct agcttttctg ataggaagca tttggtttgc caaaaagaaa taccagatta
    28861 atctagctgt cttgggcttg ggtgcagtcg ctttctttgt ctcttcgcag attttggaaa
    28921 aactggtgca tatcttgatt ttgcaccctc aaaaagacgg tagtattgcc ctcttgcaag
    28981 atcatccgct tatctatatt atctatggcc tagccatggc agcatttttt gaggaaacgg
    29041 ctcgtcttgt tttcttcaaa tggtttgaga aaaagagaag tttggaaaag gcagacgcct
    29101 tggcctatgg actggggcat ggtggcttgg agttgatttt tctaggcctt actagtttac
    29161 tcaatcttta cattgttctt tcggcagttc aaactcagaa tccacaggtt atgcaattac
    29221 tgtctgaaaa tatgttaaaa accattcagt cactttcggt ctggcagatt tatttgcttg
    29281 gttttgagcg aatcttggcg cttggtttcc aattactttt gacagtttgg gtttaccaag
    29341 ctgttcgcca gaagaaatgg atttatctcc taacagccta tggcttgcat gccttctttg
    29401 atctggcacc atctcttttc caagtaggct ggttgacaaa tccagtcttg gttgaagtga
    29461 ttctagcact ggagctcgtt ctggtcgcct atggaaccaa ggaaatcttt tgtaaaaaat
    29521 cataaaaagg ggggaacctc tttttcttat gcaaaatcca aacaaggtat ttttatggtc
    29581 gtcaaatgtc tctaaaaatg gtataatgga atgaattttg gaaaaggaag agtgacatgt
    29641 ctgtaagaga aaaaatgctt gaaatcttag aaggaattga tatccgtttt caggaaccct
    29701 tgcatagcta tagttataca aaagtaggtg gagaggctga ttatttggtc tttccacgaa
    29761 atcgttttga gttggctcgc cttgtgaaat ttgccaacca agaaaatatc ccttggatgg
    29821 ttcttggcaa tgcaagcaat atcatcgttc gtgatggtgg gattcgtgga tttgtcatct
    29881 tgtgtgacaa gctcaataac gtttctgttg atggctatac cattgaagca gaagctgggg
    29941 ctaacttgat tgaaacaact cgcattgccc tccgtcatag tttaactggc tttgagtttg
    30001 cttgtggtat tccaggaagc gttggcggtg ctgtctttat gaatgcgggt gcctatggtg
    30061 gcgagattgc tcacatcttg cagtcttgta aggtcttgac caaggatgga gaaatcgaaa
    30121 ccctgtctgc taaagacttg gcttttggtt accgccattc agctattcag gagtctggtg
    30181 cagttgtctt gtcagttaaa tttgccctag ctccaggaac ccatcaggtt atcaagcagg
    30241 aaatggaccg cttgacgcac ctacgtgaac tcaagcaacc tttggaatac ccatcttgtg
    30301 gctcggtctt taagcgtcca gtcgggcatt ttgcaggtca gttaatttca gaagctggct
    30361 tgaaaggcta tcgtatcggt ggcgtagaag tgtcagaaaa gcatgcagga tttatgatca
    30421 atgtcgcaga tggaacggcc aaagactacg aggacttgat ccaatcggtt atcgaaaaag
    30481 tcaaggaaca ctcaggtatt acgcttgaaa gagaagtccg gatcttgggt gaaagcctat
    30541 cggtagcgaa gatgtatgca ggtggtttta ctccctgcaa gaggtagtgg ggacctgaca
    30601 gagccccgat cggttaatct atgaaaaaga aggaatttat gccaattgaa aaaaccaatt
    30661 atcgaattca aaaacgtctc taaagttttt gaagacagca acaccaaggt tctcaaagac
    30721 atcaactttg agttggaaga agggaaattc tacacccttc taggtgcatc tggttcgggg
    30781 aaatcaacta tcctaaacat tattgcaggt ttactggatg cgacgacagg agatatcatg
    30841 ctagacggtg ttcgtatcaa tgatattcca accaacaagc gcgacgtaca taccgtcttc
    30901 caatcctatg ccttgttccc acatatgaat gtgtttgaaa atgttgcctt tccacttcgc
    30961 ttgcgtaaaa ttgataagaa agaaatcgag cagcgtgtag cggaagttct caagatggtt
    31021 cagttggaag gttatgaaaa acgttccatc cgcaaacttt ctggaggaca acgtcagcgt
    31081 gtggccatcg cccgtgctat catcaaccaa ccccgtgtgg tcttgttgga cgagccttta
    31141 tcagcgctgg acttgaaatt gagaacagac atgcagtacg aattgcgtga attacaacaa
    31201 cgattgggca ttacctttgt ctttgtcact cacgatcagg aagaagctct tgccatgagt
    31261 gactggattt tcgttatgaa tgatggcgag attgtccagt ctggaacccc tgtggacatc
    31321 tacgatgagc caatcaacca ctttgttgcc acctttatcg gggagtcaaa catcttgcca
    31381 ggtaccatga ttgaggacta cttggtcgaa tttaacggca aacgctttga agcggttgat
    31441 ggtgggatga agccaaatga acctgttgag gtcgttattc gtccagagga cttgcgcatt
    31501 acccttcctg aagaaggcaa actccaagtt aaggtcgaca cccagctctt ccgtggagtt
    31561 cattatgaaa ttatcgccta tgacgaactt ggaaatgaat ggatgatcca ctcaacccgt
    31621 aaggccatcg tgggtgagga aatcggtctg gactttgaat cagaagacat ccacatcatg
    31681 cgtctcaatg aaaccgaaga agagttcgat gctcgtatcg aagaatacgt ggaaatcgaa
    31741 gagcaagaag caggtctgat caatgcgatc gaggaggaaa gagatgaaga aaacaagctc
    31801 taaactcttt gtagtgccct acatgctttg gattgccctc tttgtattgg cacccttggt
    31861 cttgattttc ggacaatcct ttttcaacat tgaaggccag tttagtttag aaaattataa
    31921 atcttacttt gcgtcacaaa acttgaccta cctcaaaatg agtttcaact cagtgcttta
    31981 tgcagggatt gtgacctttg tgaccttgct tatcagctat ccaacagccc tctttttgac
    32041 ccgtctcaag caccgtcaac tctggctcat gctgattatc cttcctacct ggatcaattt
    32101 gctccttaaa gcctatgctt ttatcgggat ttttggtcaa aatggctcta ttaaccaatt
    32161 cttggaattt atcggaattg gttcacaaca gttgcttttt accgattttt cctttatctt
    32221 tgtcgcaagc tacatcgagc tcccctttat gattttgccg attttcaatg tcttagacga
    32281 catggataat aatctcatca atgctagtta tgaccttggt gcaactaagt gggagacctt
    32341 ccgtcatgtc atcttccctc tatctatgaa cggtgtgcga agtggggttc agtcggtctt
    32401 tatcccaagt ttgagtctct tcatgctgac ccgtttgatt ggtgggaacc gcgttatcac
    32461 cttggggacg gctattgagc agaattttct aaccaatgac aactatggta tgggttcaac
    32521 catcggtgtg attctcatcc tgaccatgtt catcaccatg tgggtgacta aggaaaggag
    32581 agaacgatga aaaaatttgc caacctttat ctgggactgg tctttctggt cctctacctg
    32641 cctatctttt acttgattgg ctatgccttt aatgctggtg atgatatgaa tagctttaca
    32701 ggttttagct ggactcactt tgaaaccatg tttggagatg ggagactcat gctgattttg
    32761 gctcagacat ttttcttggc cttcctatca gccttgatag cgaccattat cgggactttt
    32821 ggtgccattt acatctacca gtctcgtaag aaataccaag aagcctttct atcactcaat
    32881 aatatcctca tggttgcgcc tgacgttatg attggtgcta gcttcttgat tctctttacc
    32941 caactcaagt tttcacttgg ctttttgacc gttctatcta gtcacgtggc cttctccatt
    33001 cctatcgtgg tcttgatggt cttgcctcga ctcaaggaaa tgaatggcga catgattcat
    33061 gcggcctatg acttgggagc tagtcaattt cagatgttca aggaaatcat gcttccttac
    33121 ctggctccgt ctatcattac tggttatttc atggccttca cctattcgtt agatgacttt
    33181 gccgtgacct tctttgtaac aggaaatggc ttttcaaccc tatcagtcga gatttactct
    33241 cgtgctcgca aggggatttc cttagaaatc aatgccctgt ctgctctagt ctttctcttt
    33301 agtattatcc tagttgtagg ttattacttt atctctcgtg agaaggagga gcaagcatga
    33361 aaaaaatcta ttcattttta gcaggaattg cagcgattat ccttgtcttg tggggaattg
    33421 cgactcattt agatagtaaa atcaatagtc gagatagtca aaaattggtt atctataact
    33481 ggggagacta tatcgatcct gaactcttga ctcagtttac agaagaaaca ggaattcaag
    33541 ttcagtacga gacttttgac tccaacgaag ccatgtacac taagataaag cagggtggaa
    33601 cgacctacga tattgccatt ccaagtgaat acatgattaa caagatgaag gacgaagacc
    33661 tcttggttcc gcttgattat tcaaaaattg aaggaatcga aaatatcgga ccagagtttc
    33721 tcaaccagtc ctttgaccca ggtaataaat tctccatccc ttacttctgg ggaaccttag
    33781 gaattgtcta caacgaaacc atggtagatg aagcgcctga gcattgggat gacctttgga
    33841 agccggagta taagaattct atcatgctct ttgatggggc gcgtgaggtg ctgggactag
    33901 gactcaattc cctcggctac agcctcaact ccaaggatct gcagcagttg gaagagacag
    33961 tggataagct ctacaaactg actccaaata tcaaggctat cgttgcggac gagatgaagg
    34021 gctatatgat tcagaataac gttgcaatcg gcgtgacctt ctctggtgaa gccagccaaa
    34081 tgttagaaaa aaatgaaaat ctacgttatg tggtaccgac agaggccagc aatctttggt
    34141 ttgacaatat ggtcattccc aaaacagtta aaaaccaaga ctcagcctat gcctttatca
    34201 actttatgtt gaaacctgaa aatgctctcc aaaatgcgga gtatgtcggc tattcaacac
    34261 caaacatacc agcgaaggaa ttgctcccag aggaaacaaa ggaagataag gccttctatc
    34321 ccgatgttga aaccatgaaa cacctagaaa tttatgagaa atttgaccat aaatggacag
    34381 ggaaatatag cgacctcttc ctacagttta aaatgtatcg gaagtaggag ataaacgtaa
    34441 gaagacgaat cagtcaagct ggttcgtttt taaggaatag aagtcagttt ttagagccct
    34501 atctttttct attctagttg tttatttgct tatttgcttg cctagtagta tactaagcct
    34561 atactcttca aaccacgtca gcgttgcctt gccgtactca agtacagctt gaggctagct
    34621 tcctagtttg ctttttgatt tttattgagt attaacctta cagaaagcgg caacaaataa
    34681 tgaaaatgtc tacttttttc aacaaaagtt tctggccaac ctttatgatt gttaaccaaa
    34741 ctgctatact attccattta aaggatggct tggatcgaca atacctaaca actgagtcca
    34801 tatattgggt gataggcacc tttatatttg gaaatattct agtagctgtg tttagtaata
    34861 tgaaaatttg ggataaaaag aaaaacggca gtaagaaaaa atatatcctg gaaaagtaat
    34921 cagagatgta gggaattggt ctaattcaac ggagatcatt tccagttgac gcaagattaa
    34981 aaaaacgata taataagaaa gttgagaatt gattttcact tgtcttagtc cagagaaatg
    35041 gcggtgctgc gagccatcta agctaggaag tcatgctact caatcataca attgcataag
    35101 aacaagagaa tgacaagttc attgaatgaa ggtggtaccg cggtttttcg cccttcgtga
    35161 tatgagcttg tcttttgatt tttggaggtg ttgatgaaaa catttcttgt caaacaaaag
    35221 tttcgtcttg gaggcgaacg cttcgctatc aaggatgaca ggggagaaat tgcctatcag
    35281 gtggagggat cattttttaa gattcccaaa acttttacca tctatgatgc ggctggtgaa
    35341 caagtcagtc agatcagtaa agaaatcttg accttgcttc ctcgttttga gattcagctt
    35401 ctggatggct cgagttttgt cattcgtaag aagttgacct tttggcgaga taagtatgag
    35461 tttgataatc taggtcttcg tatcgagggc aatatctggg atttgaattt caaattgctg
    35521 gatgatcgcg atcagttgat tgcggaaatt aagaaggaac tcttccatct gacctctacc
    35581 tataccgtaa cggttcttga agacgcttat gcagacctag tcatttccct ctgtgtcgcg
    35641 attgactatg tggaaatgct ggaaagccaa tcacattaaa caagtaaata aggagacaat
    35701 atgaaacaac tatctagtgc acaagtacgc caaatgtggc ttgatttctg ggcgaccaaa
    35761 ggtcactcag tagaaccatc agtaagtttg gttcctgtaa atgacccaac tcttttgtgg
    35821 atcaactctg gggtagcaac ccttaagaaa tactttgacg ggaccattat ccctgaaaat
    35881 ccacgtatta ccaatgccca aaaggctatc cgtaccaacg acatcgaaaa cgtagggaag
    35941 actgcgcgtc accataccat gtttgaaatg ttggggaact tctctatcgg ggattacttc
    36001 cgtgacgaag ctatcacttg ggcttatgag cttttgataa gccctgaatg gtttgatttc
    36061 cctgctgaaa aactttacat gacctactat ccagacgata aagattctta caaccgctgg
    36121 attgaagtgg gagtggatcc aagtcacttg attccaattg aggacaactt ctgggaaatc
    36181 ggtgcgggac cttctggacc agatacagaa atcttctttg accgtgggga agcctttgac
    36241 ccagaaaata tcggtcttcg cctgcttgca gaagatattg aaaacgaccg ttatattgaa
    36301 atctggaaca tcgttttgtc acaatttaac gcagaccctg ctgttcctcg tagcgaatac
    36361 aaggaattgc cacataagaa cattgatacg ggcgctggtt tggagcgttt ggtggccgtt
    36421 atccaagggg ctaagaccaa ctttgaaacg gacctcttca tgccgattat ccgtgaagtc
    36481 gagaaattgt ctggtaaggt ttatgaccaa gatggcgaca acatgagctt caaggtcatc
    36541 gctgaccaca tccgttcact ttcatttgcc atcggtgatg gtgcccttcc aggaaatgaa
    36601 ggtcgtggtt atgttcttcg tcgtcttctc cgtcgtgctt ctatgcatgg tcaaaaattg
    36661 ggaatcaacg agcctttcct ttacaaattg gttccaactg ttggaaaaat catggaaagc
    36721 tactacccag aagtgcttga gaaacgtgac tttattgaga aaatcgttaa gagcgaagaa
    36781 gaatcatttg cccgtaccct tcactcaggt caacactttg cccaaggcat tgtagcagac
    36841 ttgaaagaaa aaggtcaatc tgttatcgct ggttcagatg tcttcaaact ctatgatact
    36901 tatgggttcc cagttgaatt gactgaagaa attgctgaag aggctggtat gactgtagac
    36961 cgtgaaggtt ttgaagcagc catgaaagaa caacaagaac gcgcgcgtgc gtcagctgtc
    37021 aagggtggct caatgggtat gcaaaatgaa actcttcaaa acatcactgt agaaagtgtc
    37081 ttcaactaca atactagcca attgtcttct aaattggtag ctatcgttgc tgacaatgca
    37141 gaagtaggag ctgtttcaga aggaactgcc tctcttatct ttgcggaaac gtcattttat
    37201 gctgaaatgg gtggacaggt agctgactac ggacaaatct tggatgagtc aggtaagatt
    37261 gtggctactg tgaccaatgt tcagaaagcc ccaaatggtc aagcccttca tacagttgaa
    37321 gtccttgcac cgcttgcctt gaaccaagaa tataccttgg caattgatag caatcgccgt
    37381 caccgtgtca tgaaaaacca cactgcgact catttgcttc acgctgccct tcacaatatc
    37441 cttggaaacc acgcaacaca ggcaggatct cttaacgaag ttgaattcct tcgctttgac
    37501 tttacccact tccaagcagt aactgctgag gaacttcgtg cgattgaaca gcaagttaac
    37561 gagaaaattt gggaagcact tgaagtgaag acagttgaaa cggatattga cactgctaaa
    37621 gaaatgggag ctatggccct ctttggtgag aaatacggca aggaagttcg tgtcgtgact
    37681 atcggtgact actctattga actttgtggt ggtactcatg ttggcaacac ttctgagatt
    37741 ggtctcttca aaattgtcaa agaagaagga atcggttcag gaactcgccg tatcttggca
    37801 gtgactggta aggaagcctt tgaagcctat cgtgaacaag aggatgctct taaagctgtc
    37861 gcagcaacct tgaaagcacc tcaagtcaag gaagtacctc acaaggtaga aggacttcaa
    37921 gaacaacttc gtcaacttca aaaagaaaat gctgagttga aagaaaaagc cgcagctgca
    37981 gccgcaggcg atatcttcaa agatgctaag gaagtcaacg gtcatcgtta cattgctagt
    38041 caagtgtctg tatccgatgc cggtgccctt cgtacttttg cagataactg gaaacaaaaa
    38101 gactactctg atcttcttgt cctagttgcc gctatcggtg acaaagtcaa tgtccttgta
    38161 gcaagcaaga caaaagacct tcatgcagga aaccttgtca aagaattagc accaatcatc
    38221 gatggacgtg gtggtggtaa accagacatg gccatggcag gaggaagcaa ccaagctaag
    38281 atccaagaat tgttggatgc agtagcaggt aaattgtaat caagtaaaga tctatccatt
    38341 tgggtaggtc tttttgtgaa tacaaaaaag ccaaatccgc ttggacctag cttggttatt
    38401 atgagctatt agatggtatt gactgcccag acacttacgg atctagctga gacagggaat
    38461 tgtccataac cttcctcatc aattgtaact tgaccttggt ggttaccaag taaatctaca
    38521 aaggtttgat tagtccattc ttgaccgaca aacattgact tgctgttttc ttggtcattt
    38581 gagataagga ctgcgattgg ggattgattt tcagcacctg aacgtaccca accgatacag
    38641 ttagcatggt caaagtagtc attttgttct ccataggcca agtcttttcg gatggctagg
    38701 aggcggtcaa ggatttcttt gaaatcttct tgagcatact ggcctgaaat cccatagtag
    38761 tctccgtaaa agacacatgg aaggccgtct tggcgtaaca aaatgagggc ataggctgct
    38821 ggcttgaacc attcttcaac ggtagactca agggcttgtc ctcgttgggt atcgtggttg
    38881 tcgacaaaag tcacagcctt gtcaggcttg agttcaacca ggctatctgt gaaaatgcca
    38941 cgaaggtcat agtttgcgcc agcttgactg gcttcaaaga gattctggtg gagacgaaca
    39001 tcgacaaggt caaagtgttc ttccgttttt tcgagatagt ccagattggc ttccttgtct
    39061 gggttccaaa attcaccaaa aacatagaaa tcgtcaccgt atttttcctt catatcgcgg
    39121 atgaagttgc gcataaagaa agagtcaata tgcttaacgg catccaaacg gaaaccagct
    39181 acaccagtcg tttccatgaa ccaatcagcc cagtcataga tgttttggat gacttcagga
    39241 tgtttaaagt ctaggtcggc atacatgagg tagtcgtagt ttccgttttc gttatcgacc
    39301 aattcctcgt tagcccagcc cttgttgtcc ccttggatca gataaatccc agatttactg
    39361 cgtttggcat cgtagtctgt accggtgaag tggtaccaat gccagtggaa gccattatag
    39421 gtatcttggc gaccatcgaa ggtaaaacta gtccaaccat tgatggtgaa gggttctcca
    39481 agttcaactg tacggtctac aggatcaact tcgataacct gaaaggcttc cctgttatcg
    39541 gcagcagcct tgtggttgag aactacatcg gccataggtt gaattccctg ttctttaagg
    39601 gcttgaatgg cttgaagata gtcttctttg aaaccatact tggtgcggac agtccctttt
    39661 tggttgaact ctcctaagtc aaataagtca tagaccccat agccgacatc tttttcgttg
    39721 gttgccttga aggctggtgg catccagacg tgactgatcc ccagatgagc taggtgtgga
    39781 gcattttcag ccagacgcgt ccagtgttga ccgtcgtggg gcagatacca ttcaaagtat
    39841 tgcatgagtg tttgattttg catgatgttt cctcttactt gtcaatcttg ttctttattc
    39901 tatcataaag tatctctcgg cgcaaacgtt tgcgtaagat agaaggaaac tattttaact
    39961 acttaaaata aagtattgat ttttttgtat caaagtgcta gtatagcgat gtataataca
    40021 gataaagata ggagttgtcc catgattgaa tttcaaaatg ttagtaagtt gtatggtgat
    40081 aaagaggcct tgagtaatct caatttgcag attgaaaatg gagagattat gggcttgatt
    40141 ggtcataatg gggctggaaa atcgaccacc ataaaatccc tagtcagtat catttcaccc
    40201 agcagtggtc gtattttggt agacggtcag gagttatcgg aaaatcgctt ggcgatcaaa
    40261 cgaaagattg gctacgtagc agactcgcct gacttatttt tacgcttaac ggccaatgaa
    40321 ttttgggaat tgatcgcttc atcctatgat ctgactagct ctgacttgga gactagtcta
    40381 gctaggctat tgaacgtttt tgattttgct gaaaaccgct atcaggttat tgaaactctt
    40441 tctcacggaa tgcgtcagaa agtctttgtc attggggcac tcttgtctgc tcctgatatt
    40501 tgggttttgg acgaaccctt gactggtttg gatccccagg ccgcctttga tttgaagcaa
    40561 atgatgaagg aacatgcaca aaaaggcaag acggtcttgt tttcaactca tgtccttgag
    40621 gtagccgagc aagtctgtga tcggattgct attttgaaaa aggggcattt gatctattgt
    40681 ggtagtgtag agaacttgag gaaagaccac ccagaccagt ttttggaaag tatctacctt
    40741 agtcttgctg gtagaaaaga ggaggttgcg gatgcgtctc aaggtcatta aaaaattagt
    40801 tgatatcaat atcctctatt catctcaaga agctaatctg gctaatctac gaaagaagca
    40861 ggctaagaat cctgggaaaa aagtaaatgt ttctgctaga gtcctaagtt cttacatttt
    40921 ttccagtctc ttgatgctca tctgttttag taatatagcc attcattttc cttttgagga
    40981 aatacctatt tattttagct cgatggttgc tattttactg gtgctggcct tttcaacttc
    41041 tttcactgcc ttttacaatg tcttttatga gagtaaggac ctggcctcct ataggcccta
    41101 tgcctttaaa gaatcagaga ttataatggc caaaggtctg tctgtcctct tgccagctct
    41161 aactggaatt gtaccaatcc tagcttattt tctagccctc tatattagtc tagctccttc
    41221 tctttggctg ggtttgcctt tgatgctact gtccttgacc ttattatttg tctctgttac
    41281 tttagtgacg gtagtggcag tacatttctt ggctcagact agggtcttca gaaagtatca
    41341 gtctattttt tcgaatgtga tgattgggat aggagttctc atacctttaa tatttatctt
    41401 ctttcttcag tcgacttttg gaagtattgt tgacaaagtt agagacattc catttctcct
    41461 ttatcctctt catatctttt acaaaatagc agtggagcct ttttcgacag aagccttagt
    41521 gggtctgctc gcttggatag gactaactct cttcctgctt tatctgacca aaaagaaggt
    41581 ccttcctcgt ttttatgacg tgatcctgct taacagtgag gagaaggtca aaaaagaacg
    41641 tcgcagcaag gagaggattt caactactaa aaagggcttt ttccgtatgg ttttacgcta
    41701 ccacctcacc ctcttgggat aggggactgg tgtgattaca gtgcttttta caagtgcttt
    41761 ccttccttat ctcatgatga tcagtctgat ttccaaaatc cgagattctc agatagttcc
    41821 agacattcat cctccatact ggttaccctt gttttttgta ggagtgttta tagcagttgt
    41881 caataacaat atcaccagcc tgcattcaat tgccttgtcc ttggagaggg aaaatgttga
    41941 ttttctgaag agtttaccct ttgactttgc tcgttatgtg aaagtgaaat tttggattat
    42001 ctttgctgtt cagtcctttt taccagttct gactttgctc ggtctttctc tatatctagg
    42061 cttgtccatc ctttcgatga tttaccttct tgtagtctgg atcattgcca gtgtcatcct
    42121 ttcttgccac cattacttta aggacgttaa aaatctgtca accaattgga gtagcattac
    42181 ggacctggtg aatcgttcaa atggcatagt cgccatagtt ttattgttta tttatagtgc
    42241 aatcctgatg gcccttgtaa ttgggagcat attcttggtt cagtctctct cccctatcct
    42301 tgccatcaac ttgggagtag gagctcttat cgtcttgctt gctcttgcta tttttggcta
    42361 tcattattac ctgtcacgca tattggcaga aatagaaaaa agatgagata gttggttgct
    42421 tgcttgataa attcaattcg acaaacgctt ttatagtttg ttagaatgta gttgtaaatg
    42481 attaagattg gtaatcaaaa atatagggag gaatgttttg aataagaaat ataatatagt
    42541 tctattattg ttgtttatag tttatttatt gggatatttt tcaatttcaa aaacgttaat
    42601 tcctataatg tgtgtgatcc aagtattttt gatagaacat atatttagag ttcgaaatag
    42661 aatgatgcaa ataggtgaaa ttttaattat tgttgcttct ataatattat ttattgatag
    42721 tatattgagt ttgtaacaaa aggcttttct ataaaatcaa attttccaga tatctactga
    42781 agcagattta tttcttcatc tttcttctac tacctttgtc actaaatttg acagacacat
    42841 attgctgatt caacctctct acctataatc tcacagttaa cctgaggcta gtttcatagt
    42901 ttgttatttg attttcgttg ataaaaagaa atgctcctcc aagacggaag agcatttttt
    42961 attgtgattc ttacacattt acttcctagt tttgcaggaa gtctagtata ccatttttta
    43021 aaagaagagt aaaaaagcaa gggtagtttc ttttttgaga attgtctaaa agtttgatat
    43081 aatggtagtt atgaatagaa taagagtcag caaaagggtt gaaaagaagc ttgctaaggg
    43141 gctagtttta ctagaagcca gtgatcttga gaatgtcaat cttaaggatc aggaagtaga
    43201 ggtgcagggt caggaaggaa actttcttgg gactgcctac ctttctcagc aaaacaaggg
    43261 cttgggctgg tttgtcagca aagacaaggt ggccttcaat caagctttct ttgaaacgtt
    43321 gtttagaaaa gccaaagaaa agagaaacgc ctactatcaa gatgatttga caactgcctt
    43381 tcgtctcttt aatcaagagg gagatggctt tgggggtctg acagtggacc tttatggcga
    43441 ctacgccgtc ttttcttggt ataactctta tgtttatcag attcgtcaga ctatatcaga
    43501 agcctttaga caggttttcc ctgaggtttt aggggcttat gagaaaatcc gctttaaggg
    43561 tttggactat gaatctgccc atgtttatgg tcaagaagca cctgactttt tcactgtttt
    43621 agaaaatggt gtcctgtatc aagtctttat gaatgatggc ttgatgacag gaattttcct
    43681 agaccagcat gaggttcgcg gtagtttagt tgacggcttg gctatgggta aatccttgct
    43741 caatatgttt tcctacacag cggctttttc agtagctgcg gccatgggag gagctagcca
    43801 gacaacttct gttgatctag ccaaacgttc acgagaattg tctcaagcgc attttcaggc
    43861 aaatgggctc agcacagacg agcgtcgttt tatagtcatg gatgtctttg agtatttcaa
    43921 atatgccaaa cgcaaaaact tgacctacga tgtgattgtc ctagatccgc ctagctttgc
    43981 tcggaataaa aaacaaactt tctctgtggc caaggattat cacaagttga tttcccagag
    44041 tcttgagatt ttaaatccgg gagggattat cattgccagt accaatgctg ccaatgtttc
    44101 ctgtcagaaa tttacagaac aaattgataa aggctttgca ggaagaagtt accagatttt
    44161 aaacaaatat ggtcttccag cagattttgc ctataataaa aaagatgaaa gtagtaatta
    44221 cctcaaggtg attagtatga aggttagtaa atgaaattaa tcgtttcagt aatgccaaga
    44281 agtttagagg aggctcaggc tctggatgcc acgaggtacc tggatgccga catcattgaa
    44341 tggcgtgccg actatctgcc taaagaagcg attttgcagg tagctccagc catttttgaa
    44401 aaattcgcag gccgtgagtt ggttttcacg ctacgaactc gctccgaagg tggagaaatc
    44461 gacctttctc cagaagagta tatccatcta atcaaggaag ttgcgcaact ctatcaacca
    44521 gactatattg attttgagta ctatagctac aaggatgttt ttgaggaaat gctggacttc
    44581 ccaaatcttg ttttgagtta ccataatttc caagaaacac ctgagaacat gatggaaatc
    44641 ttgtcagagt tgacgatcct aaatccaaaa cttgttaagg ttgcggtgat ggctcacacg
    44701 gagcaggatg tcttagactt gatgaactat acacgaggct ttaaaacgct caatcctgaa
    44761 caggaatatg tgaccatttc tatgggtaag gtgggcaagg tctctcgtat cactgcggat
    44821 gtgactggtt cgagttggtc ttttgccagt ctggatgagg ttagtgcccc tgggcagatt
    44881 tctctagcta gcatgaaaaa aatcagggag attttggatg aagcttgatg gctatacacg
    44941 tttagctgca gttgttgcca atcctattaa gcattctatt tctcccttca tccacaatag
    45001 agcctttgag gcgacagcta ccaacggtgc ttatgtggct tgggagattg aagcgagtga
    45061 cttggtagaa acagtggcca atattcgtcg ctaccagatg tttggcatca atctgtccat
    45121 gccctataag gagcaggtga ttccttattt ggatgagctg agcgatgaag cgcgcttgat
    45181 tggtgcggtt aatacggttg tcaatgagaa tggcaattta attggatata atacagatgg
    45241 caagggattt tttaagtgct tgccttcttt tacaatttca ggtaaaaaga tgaccctgct
    45301 gggtgcaggt ggtgcggcta aatcaatctt ggcacaggct attttggatg gcgtcagtca
    45361 gatttcggtc tttgttcgtt ccgtttctat ggaaaaaaca agaccttacc tagacaagtt
    45421 acaggagcag acaggcttta aagtggattt gtgtgcttta gaatatgttt ctgaactgca
    45481 agcaaggatt gccgagtcgg atttgctagt taatgccacc agtgtgggca tggatggcca
    45541 atcctctcct gttcctgaaa acatagtctt accagaaact ctcttagttg cagatatcat
    45601 ataccaaccc tttgaaacac cgtttttgaa atgggctaga agtcagggca atccagcagt
    45661 caatggtctg ggaatgttgc tctatcaagc tgcagaagct tttcaactgt ggacaggcaa
    45721 gaaaatgccg acagaagaga tttggcagtc tttaacagaa aaatatcaat aaagaaaggg
    45781 agactctcct atgaaaatca gaatcgatat tcctcatcat ccttatgata ttcagattga
    45841 aaaaggttgt atggcccagg ctggtcagtg gttgcgagaa ctctggcaac cgcaaaaggt
    45901 agtcattgtg acagataacc atgtagcctc tctctatgca gagaaggtca agctcagcct
    45961 agaagatgct ggttttcagg tagctgtttt tgatttctta gaaggtgaag aaagaaagaa
    46021 tttaactact gttcagaaag tctatgaatt tctagtcaag caaggtctga ctcgtagcga
    46081 tggaatcgtt gctcttggtg gtggcgttgt tggggacctg gctggctttg tagcctctac
    46141 ctatatgcgg ggtattcact ttgttcagat tccgactagt ttgacagctc aggttgattc
    46201 ttctatcggt ggaaagacag gcgttaacac accatttgct aaaaatatgg tggggacctt
    46261 tgcccaacca gatggggttc tgattgatcc acttgttctt gaaaccctcg gaaaaagaga
    46321 gttgattgaa gggatgggtg aagttatcaa gtatggcttg attgaggatc cagaactgtg
    46381 ggctctcttg acgggactga atggttctgt tgagaatatt ttggaacatg cagagacctt
    46441 gattgaacat tcttgtcagg tgaagcgcaa gatggtggtt gaagatgagt tggacaatgg
    46501 tattcgtctt tacctcaatt ttggccacac tattggccat gccatcgaag cgactgccgg
    46561 ttatggcaag gtcatgcatg gagaggctgt tgccatggga atggtacaga tttccaaggt
    46621 tgctgaggaa aaaggcctca tgccagctgg cattacccaa tctatcacag agatgtgtca
    46681 gaaattcggc ttgcctgttg actatgaaaa ttggaaagtt gacaagcttt atcaggctct
    46741 tactcatgac aagaaagcgc gtggtaacac cttgaaattg gtcttggtgc cagagcttgg
    46801 ttcagcgacc attcacccag tttctctgga agagatgaaa gactacttgg taaaataagg
    46861 agagtgtatg agatatttaa ctgcaggaga atcacacggc ccccgtctaa cagctattat
    46921 tgagggaatt ccagctggac ttccattgac agctgaggat atcaatgagg atcttagacg
    46981 ccgtcagggt ggctacggtc gtggtggtcg tatgaagatt gagagtgacc aggttgtctt
    47041 tacttcgggt gttcgccacg ggaagacgac aggggcgcct attactatgg atgtcatcaa
    47101 taaggaccac cagaaatggc tggacatcat gtctgcggag gacattgaag accgccttaa
    47161 aagcaagcgg aaaattactc atcctcgccc aggtcatgcc gatttggttg gggggattaa
    47221 gtaccgtttt gatgatttgc gaaattcttt ggagcgttca tcagctcgtg aaaccaccat
    47281 gcgggtggca gttggtgcag tagccaaacg cctcttggct gagctggata tggagattgc
    47341 caaccatgtc gtggtctttg gtggtaagga aatagatgtt cctgaaaatc tgacagtcgc
    47401 tgaaattaag caaagagctg cccagtctga agtttctatt gtcaaccaag aacgagaaca
    47461 ggaaatcaag gactatattg accaaatcaa acgtgatggt gataccatcg gtggggttgt
    47521 ggagacagtc gtcggaggcg ttccagttgg tcttggttcc tatgtccaat gggatagaaa
    47581 attggatgca agattggctc aagctgttgt ctctatcaat gcctttaaag gggtggaatt
    47641 tggtcttggc tttgaggctg gttatcgtaa aggcagccaa gttatggatg aaattctctg
    47701 gtctaaagaa gacggttata ctcgccgtac caataatcta ggtggttttg aaggtggtat
    47761 gactaatggg caacccatcg ttgttcgtgg ggtcatgaaa cccattccta ctctttataa
    47821 acctcttatg agtgtggata tcgaaaccca cgaaccttac aaggcaaccg tggagagaag
    47881 tgatccgact gctcttccag ctgcaggaat ggtcatggaa gcagttgtag caacggttct
    47941 ggcgcaagaa atcctcgaaa aattctcatc agataatctt gaggaactaa aagaagcggt
    48001 agccaaacac cgagactata caaagaacta ttaaggagtt cctatggcaa aaacaatcta
    48061 tatcgcaggt cttgggttga ttggagcctc tatggcactt ggtatcaaac gcgatcatcc
    48121 agattatgaa attttaggtt ataatcgtag tcaagcttcg agagatatcg ccttgaaaga
    48181 aggtatgatt gaccgtgcaa cggatgattt tgctagtttt gctcctttgg cagatgtcat
    48241 tatcctcagc ttgccaatca aacaaactat tgctttcatt aaggagttgg ccaatttgga
    48301 tttgcgagaa ggcgttatta tttcagatgc tggttcgacc aagtcaacca ttgtggatgc
    48361 ggcggagcag tatttggctg gcaagtctgt tcgctttgtc ggggcccatc ccatggctgg
    48421 tagtcacaag acaggggctg cttcggcaga tgtcaatctt tttgaaaatg cctattatat
    48481 ctttacacct tcaagcctga caagtcagga cacgcttaag gaaatgaagg atctgctttc
    48541 aggtcttcat gctcgtttta tcgagattga tgccaaggag catgatcgtg tcacttctca
    48601 gattagccat tttcctcata ttttggcttc tagtctcatg gagcagactg cggtctatgc
    48661 tcaagagcat gagatggcaa ggcgctttgc ggcaggtggt tttcgagata tgacccgaat
    48721 tgcggaaagc gagccaggaa tgtggacctc cattctcttg tccaatagcg agaccattct
    48781 ggatagaatt gaggatttca aggaacgttt ggaagtgatt ggtcaggcca ttagtaaggg
    48841 agatgaagag caaatttgga acttttttaa ccaagcgcgt gaacaacgcc agaccatgga
    48901 aatccataag cgtggtggtg tggatagctc ttacgacctc tatgttgacg ttcctgatga
    48961 agaagatgtc atcctgcgga ttttggaact gctacgtgga acttccttgg ttaacatcca
    49021 catcaacgag gaaaatcgtg aggatattca cggaatccta caaatttcat ttaaaaatgc
    49081 tcaagacttg gaaagagctg agcatctcat aacagaaaat accgactata cagtcgtcat
    49141 caaataagga gaagaacatg tcaaatattt acgatagtgc aaacgaactc agtcgcggtc
    49201 tacgcggatt accagaatac aaggctgtta aagcagctaa agatgcgatt gcagcagatg
    49261 ctgaggcaag caaaatcttt acagaatatc ttgcctttca agaggaaatt caaaaactag
    49321 cacagacagg tcaaatgcca gatgcgtcct tccaagctaa gatggaaggc tttggtaaac
    49381 agattcaagg aaatagcctc ttgtcagaat tctttaccaa gcaacaacaa ttggccattt
    49441 acctttctga cattgaaaaa attgtttttg aaccagtttc agaattgctg aaataagaaa
    49501 agaacgatcc taaagtcagg aatttttaag gaatttctga ctttttatga taaaataggt
    49561 aaaagtattg caagtatgag gtccagtatg aaactaaaaa caaacattcg ccatttacat
    49621 ggtagtatcc gcgtcccagg tgacaagtct atcagccacc gttccattat ctttggaagt
    49681 ttggctgagg gtgagaccaa ggtttatgat attctgcgag gtgaagacgt tctttcgacc
    49741 atgcaggttt ttcgtgacct tggtgttgaa attgaggata aagatggggt tattaccatt
    49801 caaggtgtag gcatggctgg cttaaaagcg ccacaaaatg cccttaatat gggaaattct
    49861 ggcacctcga ttcgcctgat ttcaggtgtc cttgctggtg cagatttcga agtagagatg
    49921 tttggagatg atagtctttc caaacgtcct atggaccgtg tgacccttcc actgaaaaaa
    49981 atgggcgtca gcatttcagg gcaaactgaa cgagacttgc ctccccttcg cttaaaaggg
    50041 acgaaaaacc taagacctat tcattatgag ttgccaattg cctctgccca agtcaagtca
    50101 gccttgatgt ttgcagcctt acaagctaag ggggagtcag ttattatcga aaaagagtac
    50161 acccgtaatc atactgaaga tatgttgcaa caatttggtg gtcatttaag tgtggatggt
    50221 aagaaaatca cagtccaggg gccacaaaaa ttgacaggac agaaggtggt cgtaccagga
    50281 gatatttcca gtgcagcctt ttggttagtc gcaggtttga ttgctccaaa ttctcgtcta
    50341 gtgctgcaga atgtggggat caacgaaact cgcaccggta ttattgatgt cattcgtgcc
    50401 atgggtggaa aattggaaat aactgaaatc gatccagtcg ctaaatctgc taccttgatt
    50461 gttgagtctt ctgacttgaa aggaacagag attggtggcg ctttgattcc acgattgatt
    50521 gatgaattgc ccattattgc cctgcttgcg actcaagccc aaggtgtaac agttatcaag
    50581 gatgctgagg agctcaaggt caaggaaaca gaccgtattc aggttgtggc agacgcctta
    50641 aatagtatgg gagcagatat tactcctacg gcagatggga tgattatcaa agggaaatca
    50701 gctcttcacg gtgctagagt caatacgttt ggtgaccacc gtatcggcat gatgacagct
    50761 atcgcagccc tcttggttgc agatggagag gtggagcttg accgtgcaga agccatcaat
    50821 accagctatc ctagtttctt tgatgatttg gagagcttga ttcatggcta aggtgttatt
    50881 agggtttatg ggggctggaa aatcgactat tgcaagaggc ttggacccta attaccttga
    50941 tatggatgct ctgattgaga agcgcctagg tatgtccatt gcgaattttt tcgctgaaaa
    51001 gggagaagca gcctttcgtc atgtagaatc agaagtccta gctgatttac tacaaacaga
    51061 ccaagtcgtg tcaactggag gaggagtggt tatttctcag agaaatcgtg acttactcaa
    51121 gactaataca gataacatct acctgaaagc agattttgaa accctctacc tacgtatcgc
    51181 agctgataag gacaatcagc gaccgctttt tctaaataat agcaaggaag aactagtagc
    51241 tatttttcaa gaaagacagg tttggtatga ggaagtggct agtcgggttt tggatgtgac
    51301 caagctaagc ccagaggaaa ttatagagga actaagatga aaatagctta tctaggtccc
    51361 aagggatcat tttcacacca cgttgtgcag acagcttttc ctcatgagga attgcaggct
    51421 tttgccaaca ttacagatgt catcaaggct tatgagcaag gattagtgga ctattctgtg
    51481 gtgccagttg aaaattctat tgagggtagt gttcatgaaa ccttggacta tctttttcat
    51541 caggctcaca tccaagcagt agcagaaatc gttcagccta ttcatcagca gttgatggtg
    51601 gttccaggcc atactaagat tgaaaagatt ttttcacatc cacaggcctt ggctcaagga
    51661 aagaaattca tcgatgaaca atatccagag gctcaaatcg aggtaacagc tagtacagct
    51721 tatgcggccc gttttatttc cgaacatcca gaccagccct ttgcagcagt tgcacctaga
    51781 agttctgctg aagaatatgg attggaactg attgccgagg atattcagga aatggaagcc
    51841 aatttcacac gtttctgggt tctaggagct gaaaagccta gtattccctt gcaagcacaa
    51901 actgaaaaga tgagtttggc cttgacatta cctgacaacc ttccaggtgc actttataag
    51961 gccctgtcga cctttgcttg gcgaggaatt gacttgacaa aaattgaaag tcgtccactc
    52021 aagacagcac tgggtgaata ctttttcatt atcgatgtgg attatatcga taaggacttg
    52081 gtccactttg cccaaaaaga attagaagcg attggaatcc agtataaaat tctgggtgcc
    52141 tatcctattt atccaatatc agaccatgga aaggagagaa gatgagtaaa gaaaatccct
    52201 taagtcatca tgagcagttg cgttatgatt atttgctaaa aaatattcac tatctcaatg
    52261 agagagaaaa aaatgagttt gcctatttgc aagaaaagct aactcttgct aggggaaata
    52321 gtagctctag cttggaacaa gaaagagaag agcaggttga cttaccaagc tatgcgaacc
    52381 ggagtcgctc acaatccaaa tcacaagcac tctctttccc tccaaaaaag aaaagacgga
    52441 agctccgtct taaacgaatt tttatggtga ttttttcact tttggtctgt gtggctttgg
    52501 ccatggtatt catgtttttg cgtggttacc aagatgctag cgcaaagaaa actgctgatg
    52561 cccgggcagc tcaagtagaa gtctttaatg gtcaggacac tagagatgga gttaatattt
    52621 taatcatggg tactgatggt cgaatcggcc agaacagtgt tgagacacga actgactcta
    52681 ttatggtatt aaatgtcggg ggctcagata agaaaatgaa gctagtcagt ttcatgcgtg
    52741 ataatttggt ctatatagat ggttatagtc aagtgattaa tggtagaaaa cagacagata
    52801 acaagttaaa cgtagcctac gagttaggag aacaagaggg gcaaaaaggg gcagaaatgg
    52861 ttcgccaagt cttgaaagat aattttgact tggacattaa gtactacgcc ttggttgatt
    52921 tccaggcctt tgccacagcg attgacacgc ttttccctga tggggtgacg attgatgctc
    52981 aattttcaac attgaatggg cgtcccctaa cagaagctac agtcggagat gatttatacg
    53041 ctaccgagac tgagtctcca acccaaacca ttaaagtcgg aaaacagcag atgaatggct
    53101 cgaccctgct caattatgct cgtttccgtg atgatgatga ggcggattac ggccgtacaa
    53161 aaagacagca acaagtttta acagcaattt tagagcaaat taaagatccc actaaacttt
    53221 tcactggttc tgaagctctt ggaaaggttt ttgctatgac ctcaaccaac gtaccctata
    53281 ctttcctcct aacaaacggt ttatctgttt tggatggagc aaaaaatggt attgaaaaat
    53341 tgacgattcc agaactgggt gactgggtag atgcctatga tgtttatgga ggcttgggct
    53401 tgctggttga tcaaaacaaa tatcaaacca agctcgctca aatgggcttg agataagata
    53461 atactcaatg aaaattaaag tgcaaactag gaagctagcc gcaggctgct aaaaacacag
    53521 ttttgaggtt gtagataaga ctgacgaagt cagttaccta tacctacggc aaggtgaagc
    53581 tgacgtggtt tgaagagatt ttcgaagagt ataagacaga aaaatcaaag tttgatagct
    53641 atttggatag cagtcaggct ttgatttttt acagtctgca atagattttc accccttgtt
    53701 tgtggtataa tgaaacgata cgatagaaag aataatgaac aatatgactg atttaaaagc
    53761 aattcaggct cgtagtctgg agatggctga atattttgtg gccttttgta aagaacatga
    53821 tttgctctgt tatctctgtg gcggaggcgc tattggtgcc cttcgaaaca agggatttat
    53881 tccttgggac gacgacctag acttttttat gcctcgtaaa gattatgaga aattagcaga
    53941 attatggcct cgttatgcag atgaacgtta tttcttgtca aagagtcaca aggattttgt
    54001 tgatcgtaat ctttttatta ccattcgtga caaggaaacc acctgtatca agccttatca
    54061 gcaggattta gatttgccac atggtctgac cttggatgtt ttgcctttgg attattatcc
    54121 gaaaaatcca gctgagcgga aaaaacaggt tcgttgggcc ttgatttatt cactcttttg
    54181 tgcgcaaact attccagaaa agcatggtgc tctcatgaaa tggggaagtc gcattttact
    54241 gggtttgact ccaaaatctc tccgttatcg catctggaaa aaagctgaga aagaaatgac
    54301 taagtatgat ttggctgatt gtgatggcat tacagaatta tgctcaggtc ctggctacat
    54361 gagaaacaag tacccaatca catcttttga agacaatctt ttcttgccat ttgaaggaac
    54421 agagatgcct attccaatcg gctatgatgt ctatctcaga actgcttttg gggattatat
    54481 gacgcctcca ccagcagaca agcaggtacc gcatcatgat gctgtcatcg ctgatatgga
    54541 taagtcttat acagaataca agggagaata tggtggctaa gaaaaaaatc ttatttttta
    54601 tgtggtcttt ttctcttgga ggtggtgcag agaagattct atcaaccatt gtttcaaatc
    54661 tggatctaga aaagtatgat attgatattc ttgaaatgga gcactttgac aagggatatg
    54721 aatctgttcc aaagcatgta cgcattttaa aatcccttca agattatcgc caaaccagat
    54781 ggttacgagc ttttttgtgg agaatgagaa tttattttcc aagactgact cgtcgtttgc
    54841 ttgtaaaaga tgattatgat gttgaagttt cttttaccat tatgaatcca ccactgttgt
    54901 tctctaaaag aagagaagtc aagaagatat cttggattca tggaagtatt gaagaacttc
    54961 ttaaggatag ctctaaaaga gaatcacata gaagccagtt ggatgctgcg aatacaattg
    55021 tagggatttc aaaaaagacc agcaattcta tcaaggaagt ttatccagat tatgcttcta
    55081 aattacagat aatctacaat ggatatgatt ttcagactat tctagaaaaa tctcaagaga
    55141 agatcgatat cgagattgct cctcaaagta tctgtactat cggacggatt gaggaaaata
    55201 agggttctga ccgtgtagtg gaagtgatac gattattaca ccaagaggga aaaaactatc
    55261 atctctattt tatcggggct ggtgatatgg aagaggaact gaaaaaacga gtcaaagagt
    55321 atgggattga ggactatgta catttccttg gttatcaaaa aaatccttat cagtatttat
    55381 ctcagacgaa agttctcttg tctatgtcta aacaagaagg ttttcctgga gtgtatgtgg
    55441 aggccttgag tctgggactc ccttttatct ctacggacgt tggaggggct gaggaattat
    55501 cccaagaagg acgatttgga caaatcattg agagcaatca agaggcagct caggcgatta
    55561 ctaattacat gacttctgcc tcaaactttg atgtcgatga ggctagccaa ttcattcaac
    55621 aatttacaat tacaaaacaa atcgaacaag tagaaaaact attagaggag tagcatggaa
    55681 actgcattaa ttagtgtgat tgtgccagtc tataatgtgg cgcagtacct agaaaaatcg
    55741 atagcttcca ttcagaagca gatctatcaa aatctggaaa ttattcttgt tgatgatggt
    55801 gcaacagatg aaagtggtcg cttgtgtgac tcaatcgctg aacaagatga cagggtgtca
    55861 gtgcttcata aaaagaacga aggattgtcg caagcacgaa atgatgggat gaagcaggct
    55921 cacggggatt atctgatttt tattgactca gatgattata tccatccaga aatgattcag
    55981 agcttatatg agcaattagt tcaagaagat gcggatgttt caagctgtgg tgtcatgaat
    56041 gtctatgcta atgatgaaag cccacagtca gccaatcagg atgactattt tgtctgtgat
    56101 tctcaaacat ttctaaagga atacctcata ggtgaaaaaa tacctgggac gatttgcaat
    56161 aagctaatca agagacagat tgcaactgcc ctatcctttc ctaaggggtt gatttacgaa
    56221 gatgcctatt accattttga tttaatcaag ttggccaaga agtatgtggt taatactaaa
    56281 ccctattatt actatttcca tagaggggat agtattacga ccaaacccta tgcagagaag
    56341 gatttagcct atattgatat ctaccaaaag ttttataatg aagttgtgaa aaactatcct
    56401 gacttgaaag aggtcgcttt tttcagattg gcctatgccc acttctttat tctggataag
    56461 atgttgctag atgatcagta taaacagttt gaagcctatt ctcagattca tcgtttttta
    56521 aaaggccatg cctttgctat ttctaggaat ccaattttcc gtaaggggag aagaattagt
    56581 gctttggccc tattcataaa tatttcctta tatcgattct tattactgaa aaatattgaa
    56641 aaatctaaaa aattacatta gaacgggggt gaccaagtga ttgatgggaa acgattatta
    56701 tttagtttga ccatagtcag ttatgccttg acgctagtaa gtggaattgt gtatctgttt
    56761 aataataata atgttagctt actttctact ttattgttct tgttggttag tagcttaatt
    56821 gcttgttgga atgatatcaa gtattactta atccatttta ttttctattt aaccattttt
    56881 gtatttctgg tatcaagacc gaccattgat tattttaggg atggtgcttt ggatacctat
    56941 catccaatag cctatcgttt tgcctttata gttgtcatgg tttcgattct gggcttgacc
    57001 acaggaggca ttctggctcg ttacttcata gctaggaaga aaataaaagt agcaaatata
    57061 ggaaattctc taaaagaggt ttatatcaag cggttacgct ttgtatcact aggagttttt
    57121 cttctaactt atcctttcta tttcattcgg ttatttgaac ggctcttgta tcgtttgcag
    57181 acttcctact atgcctacta tgcaaatttt gaaagtaaac tgccttattt tacctacatt
    57241 ttgtctacct ttacggtcta tgcaatgtgt atgtatctgg caaccaagcc aaagaaattg
    57301 caggccacag cagtgcttgt ctcctttatt gcagctaata ctattcattt ggcaattggg
    57361 acacgaaatc cctttatttt aagtatttta tttgcttttg tttattactt tatgcgggag
    57421 caaactgaaa aaggaaaatg gattgggttt aaagaaaagt tagcgatttt tgtaggttct
    57481 cctattctca tgttagcgat gggagtactc aattatgtac gggataatgt ccaagtttcc
    57541 catacaggtt tctgggatat cttacttgac tttatctata aacaagggac tagttttggt
    57601 gttctggctc gaggttttct atttaacagt agcctccctt accgagattt ccgtaatttt
    57661 acttttggtc ctgttcttga ttattttgca agggggagtt tgggagccat tttcggagga
    57721 aaagcctttg aacatacaac caatagtgtg gaactagcta ttgatagtaa tagttatgcc
    57781 cacaatctat cctatcttgt cttgaacaag gaatacctga aagggcatgg tatcggaagt
    57841 agttatatca tggagttgta taccgactat ggtatgattg gagtctttct gcttagtttc
    57901 ttactcggcg tattatttat agccatgctg caagtagcct atcgctcaag gacaatccta
    57961 tttgctttat ccctactcat cttgaataat ctattcttta tgccaagaag cagcttttca
    58021 gaaagtttct tcaatttatt tacaatgcaa ttctggggaa ttgttcttgt gattatattt
    58081 atagcaaaaa tgcttacaaa ggaaaactag tatctactaa acaaaggaga aaaaaatcat
    58141 gtttgaacat tattcagtag ctgatttgtt tgcaaatctt tataaaaagc gaaaagcaaa
    58201 tattttagct ctcatcgctt tatttgctct cattgctgta ccatttacaa ttaaagcagt
    58261 taggaataaa aacactgtca aagatacaac aagttattca acttatctta tctataaaat
    58321 cactcctcca aaagagtcgg ccaaaacgat tttgaatcat caaattggtg gttatagtga
    58381 tttttatggg aaattgattg atggtaattt gaatggagct tatcttttca atgatgtaga
    58441 acccagtgag ttgaaaaaaa ttgccagtga attagacacg acagaaacaa ccttgaaaaa
    58501 ttcaacgaat gactattggt ggaaaaaatt gaccgtctac tatatgattg acgatgcagg
    58561 ggttggtgtg aaaattttga catcaagtaa agatgccaat gacttgttag agaaaaaaat
    58621 tgatgggttg attgagaaat ttaaacatgc ctatgcaaat gtgaaaattg aaaaactgga
    58681 aaccatcaac tctaaagaat tgaacgcaaa tggtgaaaca gcgcttggct taaatgtgaa
    58741 aaatctgatt cttcgtttag ttgttattgg agtggtttgt gtgattttgg ttgtgatggg
    58801 aaatgtgtta gtttatctct ttaatccaac aatcaataga gtaggtgatt tttctcagta
    58861 tcaaattgat tttgtaacag agattacaac aattgctaac ctagcagatg ttttgtcata
    58921 caaaaatact ggacaggaat tgaccatcgt tagctcaaat aaagctatcc tagataaatt
    58981 gaaacagagt caagaagctt taaaaggaat gcattttgta gatttacagg atgtatcatc
    59041 tcttttggaa agagatacag tccttcttgt tgaagagtac ggagtgactc gttataagaa
    59101 atttgagcaa agtcttcaaa ttctcagaaa cttaaatcgt tctatcctcg gtgtagcaac
    59161 ctttaaatta taagtattct gatgtataag gtcttcaaaa tcggctctta gccgattttg
    59221 aaggcttttt ctatctgtct gcagaaattt tctcttggag tctcctatag aaagacccta
    59281 gggaaaatct atccgtatag aggagccttc ttttgataaa atcgtaaaaa tctagtataa
    59341 tagatagaac tgaaagtatg aggttactag caatgaaaat gaaacaaatt agtgatacaa
    59401 ctttaaaaat cacgatgtct ttagaggatt tgatggatcg tggaatggag attgctgact
    59461 ttctcgttcc tcaagaaaaa acagaagagt tcttttatgc tatcttggat gagctagaga
    59521 tgcctgatag ctttctggat acaggcatgt tgagcttccg tgtgactcca aaacctgata
    59581 aggtagatgt ctttgtgacc aagtcaaaga ttgaccaaaa tctagatttt gaagacttat
    59641 cggatttgcc agatatggaa gaattggctc aaatgtctcc agatgaattt atcaaaaccc
    59701 tggaaaaaag catcgcagac aaaaccaagg atgatatcga agccattcaa tctcttgagc
    59761 aagttgaagc caaggaagaa gagcaagagc aggctgaaca agaagctgag agtaagaaag
    59821 aaccttacat ctactacatc ctttcttttg ctaagttggc tgacttggta gcttttgcca
    59881 agacagtgac ttttgaaatg gaaacttctg aactctacaa aatgaacgag cgctattatt
    59941 tgaccatttt agtggatatt gaaaatcatc caagcccata tccagcttgg ctgttggccc
    60001 gtatgcgcga gtttgcagac gatagtgata tcagtcgctc agtcttacaa gagtatggtc
    60061 aagtcttgat gagtcacgat gcagtgctca atctgcaaaa aatcggctaa tcttttctgg
    60121 aaagctattt tcagatttta agaagagcga atcgtctgat tcgttttttc tttttatact
    60181 gaaatagtga tttactataa taggaatttt cacaaaattc tgttataatg gctatattag
    60241 aaaatttcga ggagataaag atgacagtta aaattgcttt actaggattt ggtaccgttg
    60301 caagtggtgt gcctttcctc ctaaaggaaa atggaggaaa aatcaatcaa tcagcacatt
    60361 cagatatcaa agttgctaag gtattggtca aggatgaaga tgaaaaaaat cgcttgcttg
    60421 cagcagggaa tgactttaac tttgtaacca atgtggatga tattttatca gaccaggata
    60481 ttactatcgt agtggaattg atggggcgta ttgagcctgc taaaaccttt atcactcgtg
    60541 ccttggaagc tggaaaacac gttgttactg ctaacaagga ccttttagct gtccatggcg
    60601 cagaattgct agaaatcgct caagctaaca aggtagcact ttactacgaa gcagcagttg
    60661 ctggtgggat tccaattctt cgtactttag caaattcctt ggcttctgat aaaattacgc
    60721 gcgtgcttgg agtagtcaac ggaacttcca acttcatggt gaccaagatg gtggaagaag
    60781 gctggtctta cgatgatgct cttgcggaag cacaacgtct aggatttgca gaaagcgatc
    60841 cgacgaatga cgtagatggg attgatgcag cctacaagat ggttattttg agccaatttg
    60901 cctttggcat gaagattgcc tttgatgatg tagcccacaa gggaatccgc aatatcacac
    60961 cagaagacgt agctgtagct caagagcttg gttacgtagt gaaattggtt ggttctattg
    61021 aggaaacttc ttcaggtatt gctgcagaag tgactccaac cttcctacct aaagcgcacc
    61081 cacttgctag tgtgaatggc gtaatgaacg ctgtctttgt agaatctatc ggtattggtg
    61141 agtctatgta ctacggacca ggtgcgggtc aaaaaccaac tgcaacaagt gttgtagctg
    61201 atattgtccg tatcgttcgt cgtttgaatg atggtactat tggcaaagac ttcaacgaat
    61261 atagccgtga cttggtcttg gcaaatcctg aagatgtcaa agcaaactac tatttctcaa
    61321 tcttggctct agactcaaaa ggtcaggtct tgaagttggc tgaaatcttc aatgctcaag
    61381 atatttcctt taagcaaatc cttcaagatg gcaaagaggg tgacaaggcg cgtgtcgtta
    61441 tcatcacaca caagattaat aaagcccagc ttgaaaatgt ctcagctgaa ttgaagaagg
    61501 tttcagaatt cgacctcttg aataccttca aggtgctagg agaataagat gaagattatt
    61561 gtacctgcaa ccagtgccaa tatcgggcca ggttttgact cggtcggtgt agctgtaacc
    61621 aagtatcttc aaattgaggt ctgcgaagaa cgagatgagt ggctgattga acaccagatt
    61681 ggcaaatgga ttccacatga cgagcgtaat ctcttgctta aaatcgcttt gcaaattgta
    61741 ccagacttgc aaccaagacg cttgaaaatg accagtgatg tccctttggc gcgcggtttg
    61801 ggttcttcca gctcggttat cgttgctggg attgaactag ccaaccaact gggtcaactc
    61861 aacttatcag accatgaaaa attgcagtta gcgaccaaga ttgaagggca tcctgacaat
    61921 gtggctccag ccatttatgg taatctcgtt attgcaagtt ctgttgaagg gcaagtctct
    61981 gctatcgtag cagactttcc agagtgtgat tttctagctt acattccaaa ctatgaatta
    62041 cgtactcgcg acagccgtag tgtcttgcct aaaaaattgt cttataagga agctgttgct
    62101 gcaagttcta tcgccaatgt agcggttgct gccttgttgg caggagacat ggtgaccgct
    62161 gggcaagcaa tcgagggaga cctcttccat gagcgctatc gtcaggactt ggtaagagaa
    62221 tttgcgatga ttaagcaagt gaccaaagaa aatggggcct atgcaaccta cctttctggt
    62281 gctgggccga cagttatggt tctggcttct catgacaaga tgccaacaat taaggcagaa
    62341 ttggaaaagc aacctttcaa aggaaaactg catgacttga gagttgatac caaaggtgtc
    62401 cgtgtagaag caaaataaag aatagaagat aggatgggga aactcttgac cagagggttt
    62461 catatccttt ttgtgaaaag aagtttatac tcaatgaaaa tcaaagagca aactaggaag
    62521 ctagccgcag gctgctcaaa acactgtttt gaggttgcag atagaactga cgaagtcagc
    62581 tcaagacact gttttgaggt tgcagataga actgacgaag tcagtaacat acctacggta
    62641 aggtgacgct gacgtggttt gaagagattt tcgaagagta ttagttaaaa acgtgataaa
    62701 ggagaaataa agatggcaga aatttatcta gcaggtggtt gtttttgggg cctagaggaa
    62761 tatttttcac gcatttctgg agtgctagaa accagtgttg gctacgctaa tggtcaagtc
    62821 gaaacgacca attaccagtt gatcaaggaa acagaccatg cagaaacggt ccaagtgatt
    62881 tacgatgaga aggaagtgtc actcagagag attttacttt attatttccg agttatcgat
    62941 cctctatcta tcaatcaaca agggaatgac cgtggtcgcc aatatcgaac tgggatttat
    63001 tatcaggatg aagcagattt gccagctatc tacacagtgg tgcaggagca ggaacgcatg
    63061 ctgggtcgaa agattgcagt agaagtggag caattacgcc actacattct ggctgaagac
    63121 taccaccaag actatctcag gaagaatcct tcaggttact gtcatatcga tgtgaccgat
    63181 gctgataagc cattgattga tgcagcaaac tatgaaaagc ctagtcaaga ggtgttgaag
    63241 gccagtctat ctgaagagtc ttatcgtgtc acacaagaag ctgctacaga ggctccattt
    63301 accaatgcct atgaccaaac ctttgaagag gggatttatg tagatattac gacaggtgag
    63361 ccactctttt ttgccaagga taagtttgct tcaggttgtg gttggccaag ttttagccgt
    63421 ccgatttcca aagagttgat tcattattac aaggatctga gccatggaat ggagcgaatt
    63481 gaagttcgtt ctcgttcagg cagtgctcac ttgggtcatg ttttcacaga tggaccgcgg
    63541 gagttaggcg gcctccgtta ctgtatcaat tctgcttctt tacgctttgt ggccaaggat
    63601 gagatggaaa aagcaggata tggctatcta ttgccttact taaacaaata aaacagagag
    63661 tggggcttcc cactttcttc atttctagaa tatgaataga agggatttat gaaacaccta
    63721 ttatcttact tcaaacccta catcaaggaa tcaattttag cccccttgtt caagctgtta
    63781 gaagctgttt ttgagctctt ggttcccatg gtgattgctg ggattgttga ccaatcttta
    63841 cctcagggag atcaaggtca tctctggatg cagattggcc tgctccttat ctttgcagta
    63901 attggcgttt tagtggcctt gatagctcaa ttttactcag caaaggcagc agtaggtttt
    63961 gctaaggaat tgacaaacga tctttatcgt catattcttt ccttgccaaa ggacagcaga
    64021 gaccgtctga caacttctag tttggttact cgcttgactt cggataccta ccagattcag
    64081 actggtatca atcaattcct gcgtctcttt ttacgagcgc ccattatcgt ttttggtgcc
    64141 atttttatgg cttatcgaat ctcagctgag ttgactttct ggttcttagt catggttgcc
    64201 attttgacca ttgtcattgt agggttatct cgattggtca atcctctcta cagtagtctc
    64261 agaaagaaaa cggaccaact ggttcaggaa acgcgccagc aattgcaagg gatgcgggtt
    64321 attcgtgctt ttggtcaaga aaaacgagag ttacagattt ttcaaaccct taaccaagtt
    64381 tatgctagat tacaagaaaa gacaggtttc ttgtctagtt tattaacacc tctgacctat
    64441 ctgattgtca atggaactct tctcgttatt atctggcaag gctatatttc aattcaagga
    64501 ggagtactca gtcaaggtgc tctcattgct cttatcaatt acctcttaca gattttggtg
    64561 gaattggtca agctagccat gctgatcaat tccctcaacc agtcctatat ctcagccaag
    64621 agaatcgagg aggtctttgc ggaatctccc gaaaacatcc attcagaatt agaacaaaag
    64681 caagttacca gtggtcgggt tttacaagtc caagaattga cttttaccta tcctgatgcg
    64741 gcccagcctt ctctgagaga catttccttt gatatgactc aaggacaaat ccttggtatc
    64801 attgggggga ctggttctgg taaatcaagc ttggtgcaac tcttacttgg actttatcca
    64861 gtagacaagg ggaacattga cctttatcaa aatggacgta gtcctcttaa tttggagcag
    64921 tggcggtctt ggattgccta tgtacctcaa aaggtcgaac tctttaaggg aactattcgt
    64981 tccaacttga ctttaggttt aaatcaagaa gtgactgatc aagaactctg gcaagccttg
    65041 gagattgcgc aagctaagga ttttgtcagt gataaggaag gactcttgga tgctctagtt
    65101 gaagcaggag ggcgaaattt ctcaggtgga caaaaacaaa ggctgtctat cgcacgagca
    65161 gtcttgcgcc aggctccgtt tctcatccta gatgatgcga cctcagcact ggacaccatt
    65221 acagagtcca agctcttgaa agctatccgc gaaaatctgc caaatatgag cttaatcttg
    65281 atttctcaac ggacttcgac actccagatg gcggaccaga ttctcctctt ggaaaaaggt
    65341 gagttgctag ctgttggcaa gcacgatgac ttgatgaaat ccagtcaagt ctatcgtgaa
    65401 atcaatgcat cccaacatgg aaaggaggac tagaatgaaa cgacaaactg taaaccagac
    65461 gctcaaacgt ttagtcgtag atttagcaaa ccatcccttc ctccttttcc tagcctttct
    65521 aggaactatt gcccaagttg gcttatcaat ttacctacct attctgattg ggcaggtcat
    65581 tgaccaagtc ctagtggctg gttcatcacc agttttttgg cagatttttc tccagatgct
    65641 cttggtggta ataggaaata ctctggtaca atgggccaat cctctcctct ataatcgtct
    65701 aatcttctct tataccagag atttacgaga gcgaatcatc cataaactcc atcggttacc
    65761 gattgctttt gcggatcggc aaggcaatgg agagatggtc agtcgtgtaa ccacggatat
    65821 cgaacagttg gcagctggct tgaccatgat ttttaaccaa tttttcattg gtgttttgat
    65881 gattttggtt agtattctag ccatgctcca aattcatctc ctcatgactc tcttagtctt
    65941 gctgttgacg ccactgtcca tggtgatttc acgctttatt gccaagagat cctatcatct
    66001 cttccagaag caaacagaga cgaggggaat tcagactcag ttgattgaag aatcgcttag
    66061 ccagcagact ataatccagt ccttcaatgc tcaaacagaa tttatccaaa gattgcgtga
    66121 ggctcatgac aactactcag gctattctca gtcagccatc ttttattctt caacggtcaa
    66181 tccttcgact cgctttgtaa atgcactcat ttatgccctt ttagctggag taggagctta
    66241 tcgtatcatg atgggttcaa ccttgaccgt cggtcgttta gtgacttttt tgaactatgt
    66301 tcagcaatac accaagccct ttaacgatat ttcttcagtg ctagctgagt tgcaaagtgc
    66361 tctggcttgc gtagagcgta tctatggggt cttagatagc cctgaagtgg ctgaaacagg
    66421 taaggaagtc ttgaccagtg accaagttaa gggagctatt tcctttaaac atgtctcttt
    66481 tggctaccat cctgaaaaaa ttttgattaa ggagttgtct atcgatattc cagctggtag
    66541 taaggtagcc atcgttggtc cgacaggtgc tggaaaatca actcttatca atctccttat
    66601 gcgtttttat cccattagct cgggagatat cttgctggat gggcaatcca tttatgatta
    66661 tacacgagta tcattgagac agcagtttgg tatggtgctt caagaaacct ggctcacaca
    66721 agggaccatt catgataata ttgcctttgg caatcctgaa gccagtcgag agcaagtaat
    66781 tgctgctgcc aaagcagcta atgcagactt tttcatccaa cagttgccac agggatacga
    66841 taccaagttg gaaaatgctg gagaatctct ctctgtcggc caagctcagc tcttgaccat
    66901 atcccgagtc tttctggcta ttccaaagat tcttatctta gacgaggcaa cttcttccat
    66961 tgatacacgg acagaagtgc tggtacagga tgcctttgca aaactcatga agggccgcac
    67021 aagcttcatc attgctcacc gtttgtcaac cattcaggat gcggatttaa ttcttgtctt
    67081 agtagatggt gatattgttg aatatggtaa ccatcaagaa ctcatggata gaaagggtaa
    67141 gtattaccaa atgcaaaaag ctgcggcttt tagttctgaa taagccattc tcttttgaaa
    67201 gtttatggac gaaaaaagtt gccttcgagt gacttttttg ttacaatagc tagaaaaatt
    67261 gtttactgta atactcaatg a
//
LOCUS       DAVBCN010000003        63812 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVBCN010000003 DAVBCN010000000
VERSION     DAVBCN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 63812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63812
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.003"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            212..3982
                     /gene="spuA"
                     /locus_tag="VQ497_000143"
     CDS             212..3982
                     /gene="spuA"
                     /locus_tag="VQ497_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HET2516514.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSNPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWIKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYRTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGPVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSVKEAVRNESV
                     ENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNKKEN"
     gene            complement(4082..5131)
                     /locus_tag="VQ497_000144"
     CDS             complement(4082..5131)
                     /locus_tag="VQ497_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET2516515.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(5250..7058)
                     /gene="glmS"
                     /locus_tag="VQ497_000145"
     CDS             complement(5250..7058)
                     /gene="glmS"
                     /locus_tag="VQ497_000145"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET2516516.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGTDNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNSRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVVDPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLIHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(7250..8629)
                     /gene="bglA"
                     /locus_tag="VQ497_000146"
     CDS             complement(7250..8629)
                     /gene="bglA"
                     /locus_tag="VQ497_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET2516517.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWDRDIELLLETGHTAFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPDSPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            complement(8728..10581)
                     /locus_tag="VQ497_000147"
     CDS             complement(8728..10581)
                     /locus_tag="VQ497_000147"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET2516518.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALIEKLEASLMGDGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(10594..11853)
                     /gene="rseP"
                     /locus_tag="VQ497_000148"
     CDS             complement(10594..11853)
                     /gene="rseP"
                     /locus_tag="VQ497_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HET2516519.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(11875..12678)
                     /locus_tag="VQ497_000149"
     CDS             complement(11875..12678)
                     /locus_tag="VQ497_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET2516520.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(12687..13445)
                     /locus_tag="VQ497_000150"
     CDS             complement(12687..13445)
                     /locus_tag="VQ497_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET2516521.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(13676..14248)
                     /locus_tag="VQ497_000151"
     CDS             complement(13676..14248)
                     /locus_tag="VQ497_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET2516522.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTTVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(14229..15200)
                     /gene="ruvB"
                     /locus_tag="VQ497_000152"
     CDS             complement(14229..15200)
                     /gene="ruvB"
                     /locus_tag="VQ497_000152"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET2516523.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            15361..15453
                     /locus_tag="VQ497_000153"
     CDS             15361..15453
                     /locus_tag="VQ497_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HET2516524.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            complement(16080..16496)
                     /locus_tag="VQ497_000154"
     CDS             complement(16080..16496)
                     /locus_tag="VQ497_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2516525.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(16508..17203)
                     /locus_tag="VQ497_000155"
     CDS             complement(16508..17203)
                     /locus_tag="VQ497_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2516526.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPQEMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            complement(17397..19898)
                     /locus_tag="VQ497_000156"
     CDS             complement(17397..19898)
                     /locus_tag="VQ497_000156"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET2516527.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSTEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLIRSKEIVAENNGALD
                     KVYNETVKAVTEQIDSLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAATGESISYVTWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            complement(20353..21441)
                     /locus_tag="VQ497_000157"
     CDS             complement(20353..21441)
                     /locus_tag="VQ497_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HET2516528.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(21459..22127)
                     /locus_tag="VQ497_000158"
     CDS             complement(21459..22127)
                     /locus_tag="VQ497_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HET2516529.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEVGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            complement(22146..24584)
                     /locus_tag="VQ497_000159"
     CDS             complement(22146..24584)
                     /locus_tag="VQ497_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HET2516530.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            complement(24735..26033)
                     /locus_tag="VQ497_000160"
     CDS             complement(24735..26033)
                     /locus_tag="VQ497_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2516531.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQTLGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKVQDRLAYQNEIKQS"
     gene            complement(26050..26358)
                     /locus_tag="VQ497_000161"
     CDS             complement(26050..26358)
                     /locus_tag="VQ497_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2516532.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            complement(26404..26724)
                     /locus_tag="VQ497_000162"
     CDS             complement(26404..26724)
                     /locus_tag="VQ497_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2516533.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            complement(26919..27899)
                     /locus_tag="VQ497_000163"
     CDS             complement(26919..27899)
                     /locus_tag="VQ497_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HET2516534.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNVGIVKIEIENFDTNMFKLENYVKEKYSLESLEIIPNEFDDTPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            complement(27912..28658)
                     /locus_tag="VQ497_000164"
     CDS             complement(27912..28658)
                     /locus_tag="VQ497_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2516535.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYETKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRVIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     CQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAVSDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            28780..29556
                     /locus_tag="VQ497_000165"
     CDS             28780..29556
                     /locus_tag="VQ497_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HET2516536.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
     gene            29631..29828
                     /locus_tag="VQ497_000166"
     CDS             29631..29828
                     /locus_tag="VQ497_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET2516537.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSSLSDISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            30045..30641
                     /locus_tag="VQ497_000167"
                     /pseudo
     CDS             30045..30641
                     /locus_tag="VQ497_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            30717..31166
                     /locus_tag="VQ497_000168"
     CDS             30717..31166
                     /locus_tag="VQ497_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2516538.1"
                     /translation="MCTILVSIYYKIGLSSSGVYKAVADGEMTVGLSYKDPAVKLLND
                     GANIKVVYPKEGTVFLPASAAIVKKSKNMENAKKFIDFIISQEVQDTLGTTTTNRPVR
                     KNAKTSENMKPIDKIKTLTEDYDYVIKNKSDIVKKYNEVFTDIQSKQ"
     gene            31179..32270
                     /locus_tag="VQ497_000169"
     CDS             31179..32270
                     /locus_tag="VQ497_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2516539.1"
                     /translation="MSEIKIINAKKIYHDVPVIENLNITIPKESLFTLLGPSGCGKTT
                     LLRMIAGFNSIEGGEFYFDDTKINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEP
                     LSNLEAKLRLDIRQAIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDGYALRMLALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASKIQVSEESTFEEELQKGNR
                     IRLRINTQKLNIFSADGSQNLIKGVNHGT"
     gene            32260..33912
                     /locus_tag="VQ497_000170"
                     /pseudo
     CDS             32260..33912
                     /locus_tag="VQ497_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(34015..34707)
                     /locus_tag="VQ497_000171"
     CDS             complement(34015..34707)
                     /locus_tag="VQ497_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2516540.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEIIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(34951..36288)
                     /locus_tag="VQ497_000172"
     CDS             complement(34951..36288)
                     /locus_tag="VQ497_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HET2516541.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINCAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            complement(36298..36564)
                     /locus_tag="VQ497_000173"
     CDS             complement(36298..36564)
                     /locus_tag="VQ497_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET2516542.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            complement(36829..37215)
                     /gene="rplQ"
                     /locus_tag="VQ497_000174"
     CDS             complement(36829..37215)
                     /gene="rplQ"
                     /locus_tag="VQ497_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET2516543.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(37227..38162)
                     /locus_tag="VQ497_000175"
     CDS             complement(37227..38162)
                     /locus_tag="VQ497_000175"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET2516544.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            complement(38205..38588)
                     /gene="rpsK"
                     /locus_tag="VQ497_000176"
     CDS             complement(38205..38588)
                     /gene="rpsK"
                     /locus_tag="VQ497_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET2516545.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(38606..38971)
                     /gene="rpsM"
                     /locus_tag="VQ497_000177"
     CDS             complement(38606..38971)
                     /gene="rpsM"
                     /locus_tag="VQ497_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET2516546.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(38989..39105)
                     /gene="rpmJ"
                     /locus_tag="VQ497_000178"
     CDS             complement(38989..39105)
                     /gene="rpmJ"
                     /locus_tag="VQ497_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET2516547.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            complement(39130..39348)
                     /gene="infA"
                     /locus_tag="VQ497_000179"
     CDS             complement(39130..39348)
                     /gene="infA"
                     /locus_tag="VQ497_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET2516548.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(39465..40103)
                     /locus_tag="VQ497_000180"
     CDS             complement(39465..40103)
                     /locus_tag="VQ497_000180"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET2516549.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            complement(40254..41564)
                     /gene="secY"
                     /locus_tag="VQ497_000181"
     CDS             complement(40254..41564)
                     /gene="secY"
                     /locus_tag="VQ497_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET2516550.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            complement(41577..42017)
                     /gene="rplO"
                     /locus_tag="VQ497_000182"
     CDS             complement(41577..42017)
                     /gene="rplO"
                     /locus_tag="VQ497_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET2516551.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            complement(42162..42386)
                     /gene="rpmD"
                     /locus_tag="VQ497_000183"
     CDS             complement(42162..42386)
                     /gene="rpmD"
                     /locus_tag="VQ497_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET2516552.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            complement(42358..42852)
                     /gene="rpsE"
                     /locus_tag="VQ497_000184"
     CDS             complement(42358..42852)
                     /gene="rpsE"
                     /locus_tag="VQ497_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET2516553.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            complement(42870..43226)
                     /gene="rplR"
                     /locus_tag="VQ497_000185"
     CDS             complement(42870..43226)
                     /gene="rplR"
                     /locus_tag="VQ497_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET2516554.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(43310..43846)
                     /gene="rplF"
                     /locus_tag="VQ497_000186"
     CDS             complement(43310..43846)
                     /gene="rplF"
                     /locus_tag="VQ497_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET2516555.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(44038..44436)
                     /gene="rpsH"
                     /locus_tag="VQ497_000187"
     CDS             complement(44038..44436)
                     /gene="rpsH"
                     /locus_tag="VQ497_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET2516556.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(44721..44990)
                     /gene="rpsN"
                     /locus_tag="VQ497_000188"
     CDS             complement(44721..44990)
                     /gene="rpsN"
                     /locus_tag="VQ497_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HET2516557.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            complement(45008..45550)
                     /gene="rplE"
                     /locus_tag="VQ497_000189"
     CDS             complement(45008..45550)
                     /gene="rplE"
                     /locus_tag="VQ497_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET2516558.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            complement(45574..45879)
                     /gene="rplX"
                     /locus_tag="VQ497_000190"
     CDS             complement(45574..45879)
                     /gene="rplX"
                     /locus_tag="VQ497_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET2516559.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            complement(45957..46325)
                     /gene="rplN"
                     /locus_tag="VQ497_000191"
     CDS             complement(45957..46325)
                     /gene="rplN"
                     /locus_tag="VQ497_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET2516560.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            complement(46351..46611)
                     /gene="rpsQ"
                     /locus_tag="VQ497_000192"
     CDS             complement(46351..46611)
                     /gene="rpsQ"
                     /locus_tag="VQ497_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET2516561.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            complement(46636..46842)
                     /locus_tag="VQ497_000193"
     CDS             complement(46636..46842)
                     /locus_tag="VQ497_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET2516562.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            complement(46852..47265)
                     /gene="rplP"
                     /locus_tag="VQ497_000194"
     CDS             complement(46852..47265)
                     /gene="rplP"
                     /locus_tag="VQ497_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET2516563.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(47269..47922)
                     /gene="rpsC"
                     /locus_tag="VQ497_000195"
     CDS             complement(47269..47922)
                     /gene="rpsC"
                     /locus_tag="VQ497_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET2516564.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(47935..48279)
                     /gene="rplV"
                     /locus_tag="VQ497_000196"
     CDS             complement(47935..48279)
                     /gene="rplV"
                     /locus_tag="VQ497_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET2516565.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            complement(48291..48572)
                     /gene="rpsS"
                     /locus_tag="VQ497_000197"
     CDS             complement(48291..48572)
                     /gene="rpsS"
                     /locus_tag="VQ497_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET2516566.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            complement(48676..49509)
                     /gene="rplB"
                     /locus_tag="VQ497_000198"
     CDS             complement(48676..49509)
                     /gene="rplB"
                     /locus_tag="VQ497_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET2516567.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(49527..49823)
                     /locus_tag="VQ497_000199"
     CDS             complement(49527..49823)
                     /locus_tag="VQ497_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET2516568.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(49823..50446)
                     /gene="rplD"
                     /locus_tag="VQ497_000200"
     CDS             complement(49823..50446)
                     /gene="rplD"
                     /locus_tag="VQ497_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET2516569.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            complement(50471..51097)
                     /gene="rplC"
                     /locus_tag="VQ497_000201"
     CDS             complement(50471..51097)
                     /gene="rplC"
                     /locus_tag="VQ497_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET2516570.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            complement(51313..51621)
                     /gene="rpsJ"
                     /locus_tag="VQ497_000202"
     CDS             complement(51313..51621)
                     /gene="rpsJ"
                     /locus_tag="VQ497_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET2516571.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(51887..52504)
                     /locus_tag="VQ497_000203"
     CDS             complement(51887..52504)
                     /locus_tag="VQ497_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HET2516572.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            complement(52501..53091)
                     /gene="nrdG"
                     /locus_tag="VQ497_000204"
     CDS             complement(52501..53091)
                     /gene="nrdG"
                     /locus_tag="VQ497_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HET2516573.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVAGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKEIYEQVKRE"
     gene            complement(53084..53590)
                     /locus_tag="VQ497_000205"
     CDS             complement(53084..53590)
                     /locus_tag="VQ497_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2516574.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDVEDFSYEEWL
                     EGNRNKEMGIWLPENRVPSIQFVLFGESGCALGFLNLRLRLNEGLLNYAGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            complement(53634..53774)
                     /locus_tag="VQ497_000206"
     CDS             complement(53634..53774)
                     /locus_tag="VQ497_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516575.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            complement(53786..55993)
                     /gene="nrdD"
                     /locus_tag="VQ497_000207"
     CDS             complement(53786..55993)
                     /gene="nrdD"
                     /locus_tag="VQ497_000207"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HET2516576.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNCRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            complement(56113..57651)
                     /locus_tag="VQ497_000208"
     CDS             complement(56113..57651)
                     /locus_tag="VQ497_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET2516577.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            complement(57688..57831)
                     /locus_tag="VQ497_000209"
     CDS             complement(57688..57831)
                     /locus_tag="VQ497_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516578.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            complement(57909..59441)
                     /gene="cls"
                     /locus_tag="VQ497_000210"
     CDS             complement(57909..59441)
                     /gene="cls"
                     /locus_tag="VQ497_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HET2516579.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            complement(59587..60144)
                     /locus_tag="VQ497_000211"
     CDS             complement(59587..60144)
                     /locus_tag="VQ497_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HET2516580.1"
                     /translation="MTPLFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQQVQQPAQQPAQQNTNTANAGGNQNQAAPVQNQPVAQP
                     TDIDGTYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKI
                     YTVNGNQIVVDDMDRDPSDQIVLTK"
     gene            complement(60141..61391)
                     /locus_tag="VQ497_000212"
     CDS             complement(60141..61391)
                     /locus_tag="VQ497_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET2516581.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRIQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFEMLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNFSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGALKRKD
                     YQGMLGYLTEKLPQVELKVTGFDYQGALDERDVTGYDIVSSCREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            complement(61599..61904)
                     /locus_tag="VQ497_000213"
     CDS             complement(61599..61904)
                     /locus_tag="VQ497_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HET2516582.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            complement(61920..62339)
                     /gene="ruvX"
                     /locus_tag="VQ497_000214"
     CDS             complement(61920..62339)
                     /gene="ruvX"
                     /locus_tag="VQ497_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET2516583.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPLDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            complement(62343..62609)
                     /locus_tag="VQ497_000215"
     CDS             complement(62343..62609)
                     /locus_tag="VQ497_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HET2516584.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            complement(62696..63265)
                     /locus_tag="VQ497_000216"
     CDS             complement(62696..63265)
                     /locus_tag="VQ497_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2516585.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            complement(63331..63729)
                     /gene="spx"
                     /locus_tag="VQ497_000217"
     CDS             complement(63331..63729)
                     /gene="spx"
                     /locus_tag="VQ497_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HET2516586.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
BASE COUNT        18883 a        14185 c        11396 g        19348 t
ORIGIN      
        1 ttctgaataa aaatagctgg gaacacatca tcttcctaat aatacggctt ggcttatgtg
       61 acatttttat ctccgtcatt tatactgata tataaaatag tacagaaaac ttctcaaaag
      121 taggaatttt ttcacaaatc aaagaaaacg cttgcatatt gttttactac atgctatact
      181 gatatagaaa tttattgatt ggagaatcat tatgagaaaa acaccatctc acactgagaa
      241 aaaaatggtc tacagcatcc gttccctcaa aaatggaact ggttctgtcc ttatcggcgc
      301 aagccttgtc ttgcttgcca tggctacacc aactatctca tccgacgaaa gtacaccaac
      361 cactaacgaa cccaacaaca gaaatacaac cacccttgcc caacctctta ctgatacagc
      421 agctggctct ggtaagaacg aaagtgatat ttcttcacct agaaatgcaa acgcttccct
      481 agagaaaaca gaagaaaaac ctgcaacaga gccaactact tctactagtc cagtaacaac
      541 tgaaactaag gcagaagagc ccatcgaaga taactacttc cgtatccatg tcaaaaaact
      601 tcctgaagaa aacaaggatg ctcaaggact atggacttgg gacgatgttg aaaaaccatc
      661 tgaaaactgg ccaaacggag ctttgtcctt caaggatgcc aagaaagatg actacggcta
      721 ttacctagat gtcaaattaa agggagaaca agccaagaaa attagcttcc tcatcaacaa
      781 tacagctgga aaaaatctaa ccggcgataa atctgtagaa aaactagttc caaaaatgaa
      841 cgaggcttgg ttagaccaag attacaaggt tttctcttac gagccacagc ctgcaggaac
      901 tgttcgcgtc aactactacc gcacagatgg caactatgac aagaaatctc tctggtactg
      961 gggagatgta aaaaatccaa gtagcgctca atggcctgac ggaacagact ttacggctac
     1021 aggcaaatat ggccgctata tcgacattcc tcttaatgaa gccgcaagag aatttggatt
     1081 tttattacta gatgagagca aacaaggaga cgacgtgaaa atccgtaaag aaaattataa
     1141 gttcacagat ttgaaaaatc atagccaaat tttcctaaaa gacgatgatg aatcgattta
     1201 cacaaatcca tactatgtcc atgatatccg tatgacagga gcccaacacg taggcacttc
     1261 tagcattgaa agtagctttt caacacttgt cggtgctaaa aaagaagata tcctcaaaca
     1321 ctccaacatc actaatcacc taggaaacaa ggtaactatt accgatgttg caatcgatga
     1381 agctggtaag aaagtgacct acagcggaga tttctctgac acaaaacatc cttatactgt
     1441 tagctacaat tccgaccaat tcactaccaa aacaagctgg cgcctgaaag atgagacata
     1501 cagctatgat ggcaaactgg gagctgacct aaaagaagaa ggaaaacaag ttgatttgac
     1561 cctttggtca ccaagtgctg ataaggtttc tgttgttgtc tacgacaaga atgaccctga
     1621 caaagtagtt ggaactgtcg ctctcgaaaa aggggaaaga ggaacttgga aacaaactct
     1681 agacagcaca aacaaactcg gaatcacaga tttcactggc tactattatc aataccaaat
     1741 cgagcgtcaa ggtaaaactg ttcttgcact cgatccttac gctaaatctc ttgctgcttg
     1801 gaatagcgac gatgccaaga ttgacgatgc ccataaagtg gctaaagccg cctttgtaga
     1861 tccagctaaa ctcggacctc aagacttgac ttatggtaag attcacaatt tcaagactcg
     1921 tgaagacgcc gttatctacg aagctcatgt gcgtgacttc acttcagatc ctgccattgc
     1981 aaaagacttg accaaaccat ttgggacttt tgaagccttc attgaaaaac tagactatct
     2041 caaagacttg ggtgtaaccc atatccagct ccttccagtc ttgtcttact actttgtcaa
     2101 tgaattgaaa aaccatgaac gcttgtctga ctatgcttca agcaacagca actacaactg
     2161 gggatatgac cctcaaaact acttctcctt gactggtatg tactcaagca atcctaagaa
     2221 tccagaaaaa cgaatcgcag aatttaaaaa cctcatcaac gaaatccaca aacgtggtat
     2281 gggagctatc ctagatgtcg tttataacca cacagccaaa gtcgatatct ttgaagacct
     2341 agagccaaac tactaccact ttatggatgc cgatggcaca cctcgaacta gctttggtgg
     2401 tggacgcttg gggacaaccc accatatgac caaacgcctc ctagttgact ctatcaaata
     2461 cctagttgat acctacaaag tggatggatt ccgtttcgat atgatgggag accatgacgc
     2521 cgcttctatc gaagaagctt acaaggctgc acgcgccctc aatccaaatc taatcatgtt
     2581 aggcgaaggt tggagaacct atgccggtga tgaaaacatg cctactaaag ctgctgacca
     2641 agattggata aaacataccg atactgtcgc tgtcttttca gatgacatcc gtaacaacct
     2701 caaatctggt tatccaaacg aaggtcaacc tgcctttatc acaggtggca agcgtgatgt
     2761 taacactatc tttaaaaatc tcattgctca gccaactaac tttgaagctg acagccctgg
     2821 agatgtcatc caatacatcg cagcccatga taacttgacc ctctttgaca tcattgccca
     2881 gtctatcaaa aaagacccaa gcaaggctga gaactatgct gaaatccacc gtcgtttgag
     2941 acttggaaat ctcatggtct tgacagctca aggaactccg tttatccact ctggccagga
     3001 atatggacgt accaaacaat tccgtgaccc agcctacaga actcctgtag cagaggataa
     3061 ggttccaaac aaatctcact tgttgcgtga taaggacggc aacccatttg actatcctta
     3121 cttcatccat gactcttacg attctagtga tgcagtcaac aagtttgact ggactaaggc
     3181 tacagatggt aaagcttatc ctgaaaatgt caagagccgt gactatatga aaggtttgat
     3241 tgcccttcgt caatctacag atgccttccg acttaagagt ctccaagata tcaaagaccg
     3301 tgtccacctc atcactgtcc caggccaaaa tggtgtggaa aaagaggatg tagtgattgg
     3361 ctaccaaatc actgctccaa acggcgatat ctacgcagtc tttgtcaatg cggatgaaaa
     3421 agctcgcgaa tttaatttgg gaactgcctt tgcacatcta agaaatgcgg aagttttggc
     3481 agatgaaaac caagcaggac cagtcggaat tgccaacccg aaaggacttg aatggactga
     3541 aaaaggcttg aaattgaatg cccttacagc tactgttctt cgagtctctc aaaatggaac
     3601 tagccatgag tcaactgcag aagagaaacc agactcaacc ccttccaagc ctgaacatca
     3661 agacccagct ccagaagcta gacctgattc tactaaacca gatgccaaag tagctgatgc
     3721 ggaaaataaa cctagccaag ctacagctga ttcacaagct gaacaaccag cacaagaagc
     3781 acaagcatca tctgtaaaag aagcggttcg aaacgaatcg gtagaaaact ctagcaagga
     3841 aaatatacct gcaaccccag ataaacaagc tgaacttcca aatacaggaa tcaaaaacga
     3901 aaacaaactc ctatttgcag gaatcagcct ccttgcgctc cttggtctcg gtttcttact
     3961 aaaaaataaa aaagagaact aaactagccc tcctatagaa aaatccccca agcattatag
     4021 ctcgggggat taatttttgt acaatatttg ttgtcctaat aaacttgatt aggatttttt
     4081 attaagcctc tttcatagca aaataagctc gtactttggg tgcaacttgt gttccgaaga
     4141 gttcaatagc tctcagaacc tggtcatgag gcatagaacc aagcggtaga tgaagcatga
     4201 agcggtccaa tcctaaatcc tctatcatgc gaatcaattt ttcggccacc tgatctggat
     4261 tgccaacaaa catggcgcca tttggcccta cctgctccaa atattgctca taacgcaatt
     4321 cctgccagtg cggacggtct ttggaaatag catccaccac ttgcttagtc ggatggaaat
     4381 aatctttcac cgcctgctca ccatcttccg caatccaccc ccaagaatgg gctcccactt
     4441 tcaagtcttt gtcagcatgg cccgcttcgc ttccaatctc acgataagcc tgaatcaact
     4501 ttttaaaata acgtggatta ccaccaataa tagcatatac aatcggtaga ccagcctgag
     4561 caatcttcac tgttgattcg acatgaccac ctgtagctat ccacaagggc aatttgtcct
     4621 gaactggacg aggataaact tctttaccag caatcgtttg agtcaatcga ccttgccagt
     4681 ctaacttggt cttttcattg actaactgaa gcaagtctaa tttctcatca aaaagagagt
     4741 cgtagtcttt caagtcataa ccaaacagag ggaaagattc cgtgaaagat ccccttccag
     4801 ccataatctc cgctcgtcca tttgacaaag catcgatagt ggcatactgt tggaacaaac
     4861 gaatcgggtc catgcttgac agaatgctga ctgcactggt caaacggatt ttcttggtat
     4921 tgactgcccc agcggccagg acaatctctg gggctgatac tgcaaaatcc gcccgatggt
     4981 gctcgccaat cccatatacg tccaaaccaa ccttgtcagc cagctcaatc tctgccacca
     5041 actggcgaat gcgttcagca tgactgtaag tttgtccagt cccttcaagc tccgttattt
     5101 ccccaaatgt tgaaattccc aattctacca ttgtgattct ccttgtctat ctctgtcctt
     5161 caatttgaaa aattattcta acacgaatct tgagtacaag caaccaattt gctcacaaga
     5221 aaaagcctag ataactagac ttttttagct tattctaccg ttactgactt ggcaaggtta
     5281 cgtggtttgt ccacatcgag gccacggtgg agggttgcaa agtaagcgac taattgcgtt
     5341 ggtacgacca ttgaaattgg tgagaggtat ggatgtacgg tcgtaaggac gatatcgtcg
     5401 gtatctttgg ctacattctc ttctgcgata gtgaggactt tggcaccacg ggctgcgacc
     5461 tcttggatat ttccacgagt atgattggca agaactggat ctgacaagag agccaaaaca
     5521 ggcgttcctt cttcaatcaa ggcaatggtt ccgtgcttga gttctcctgc agcaaagcct
     5581 tcacactgga tataagaaat ctctttgagt ttgagacttg cttccatggc tacgtagtaa
     5641 tcttgaccac gtccgatgta aaaggcgtta cgagttgttt caagaagttc acgaaccttg
     5701 gcttcaatgg tttctttctc tgaaagagtt gattcgatag actgagctac gattgacaat
     5761 tcatgaatca ggtcaaaggc ttgcgcttta gcattaccat ttgcttctcc gactgctttt
     5821 gcaaggaagg caagggctgc gatttgcgct gtataggctt tagttgatgc cacggcaatt
     5881 tcaggacctg cgtgaaggag catggtatag ttggcttcac gtgagagggt tgagcctgga
     5941 acatttgtca ctgttaagct tggaattccc atttcattag ccttgaccaa aacttgacga
     6001 ctatccgctg tttcaccaga ttggctgata aagatgaaga gtggtttctt gctgagaagt
     6061 ggcataccgt agccccactc agatgagatg ccaagttcaa ctggtgtatc tgtcaattct
     6121 tccaacattt tcttagaagc aaatcctgca tggtaagatg ttccagctgc aaggatgtag
     6181 atgcggtctg cgtcttgaac agccttaatg atagcaggat caaccactac ttgaccagca
     6241 tcatccgtgt aggcttgaat gagtttacgc ataacagttg gttgctcatc aatttcctta
     6301 agcatgtagt aaggataagt tcccttaccg atatctgaca agtcaagttc cgcagtatag
     6361 ctagcacgtt cacgactgtt accatcatag tcttgaactt ccacgctatc agccttgacg
     6421 attaccaact cttggtcatg aatttccatg tattggttgg tttcacgaat catagccata
     6481 gcatctgagc agaccatatt gtagccttcc ccaagaccaa tcaaaagtgg agatttgttt
     6541 ttcgctacat agatgacatc tggattttca gagtcaatca aggcaaaggc atatgaacca
     6601 cggataatat gaagagcttt tttaaaggct tcaagaactg agagaccgtc ttcttccgca
     6661 aattttccaa tcaaatgtac ggcgatttcc gtatctgttt gccctttgaa gtggtgccca
     6721 gcaaggtatt cttctttaat ttcaaggtag ttttcaatca ccccattgtg aaccaagaca
     6781 aaacgttctg tctcagagcg gtgtgggtga gcattgtctt ccgttggttt cccatgagtt
     6841 gcccaacgag tatgtccgat accagttgtt ccctcaacac cagctgtctt agcagacaat
     6901 tctgcgatac gaccaacagc cttcaccaaa tggttatcag taccatctag gacaaaaatt
     6961 cccgcagaat catagccacg gtattcaagc ttttcaagcc cttgaatcaa aatatcagtt
     7021 gcatttgtgt ttccaacaac accaacaatt ccacacatag tatatacgac acaggcaagc
     7081 tgtgctttct ccttaaaatt ggtatagtct aattcatctt ttatagaatc agcaaaaata
     7141 gtatatactt gtttctttca cttgtcaaga gtaaaaattg gtatagttca aattaagctc
     7201 ctgtaagcaa aaaactctga ccgattggga taatcagtca gagtcctttt taaaatccat
     7261 tattatcgct taattctttg aaccagtggc ctgatttctt cagacgacgt tcttgcgttt
     7321 ccaagtctaa ttcgaccaaa ccatagcgat ttttatagct gttgagccat gaccagcagt
     7381 caataaaggt ccaaatcaag tagcccttac agttggcacc atcttcaatg gcacggtgaa
     7441 gttcacgaag atgacctttt acaaagtcaa tacggtaatc atcttgaatc attccatctt
     7501 gacggaattt ttcttctcct tcaacaccca taccattctc agtcaacatc cactcaatat
     7561 tgccataatt ttccttgata ttttgggcga tgtcataaat tccttgctca taaatctccc
     7621 aaccacggtg agaattgatt ttacgtccag gcatcacata aggctcgtaa aaatgttctg
     7681 gtaagagtgg actatctgga tgcttagcaa atcgaggagc cataacacgc aaaggttgat
     7741 agtagttcac accaaggaag tccaccgtat tatcacgaat gagttccaac tcttcctctg
     7801 tagcatcagg taaaagaccg tgttcatgca agatttctac caactcctgt ggataagtcc
     7861 ccaagacaga tggatctaag aaagattggg cctgaaaaag ggccgcaata cgagctgcct
     7921 tgacatcagc aggatgctgg ctacgtggat aagccggtgt caagttgagg acaatcccaa
     7981 tcttggaatc aggcaaaagt tcatggcaag ccttaacagc ccggctgctg gccaattgtg
     8041 tatgataggc taccttaaca gctgcctctg catccacctt atgtggataa tgggcatcat
     8101 aaaaataacc aaattctaca ggaacgatgg gctcgttaaa ggtaatccat tgatccacta
     8161 aatctccata agtctcaaaa caaaaacgag catagtcttc ataggctgag actgtcgcct
     8221 tattttccca accatcacca tcctcttgaa gggcaaaagg taaatcaaaa tgatagagat
     8281 tgactaacag acgaattcct ttagccttaa tagcctcaaa gaccttacga taaaaatcca
     8341 caccttgagg gttgactttt ccacagcctt gtggaaaaat ccgtgaccac tgaatagaag
     8401 tccgaaaggc tgtgtgacca gtctctaaca aaagctcaat atcccgatcc caattttcat
     8461 aaaaagtcga tgtcttatct gaaccaatcc cattatagta acgatttggc tccacttgga
     8521 accagtaatc ccagagattg tctcccttgc cgtcaccagc tacacgtcct tctgtctgcg
     8581 gtccagaagt agaggatccc cagacaaaat cctttggaaa tcttagcata catttacctc
     8641 tttatctact catttctccc attatacaga aaaaacaagg taaaaactag ttacattttt
     8701 tccttgtttt tcttctgatt atagttttta tttcttgctt aggatttcaa gcgtttcaag
     8761 cacgttatct gcatgaacct caatggtgtc accagttgcc ttgatcttaa cttctacaat
     8821 gccatcggcc gcttttttac caacagtgat acggattgga agaccaatca agtcactatc
     8881 gctaaattta acaccgacac gttcgttacg gtcatctgtc aagacttcat aaccatctcc
     8941 catcaagctt gcttcaagtt tttctatcaa ggcttgcgct tcttcatcct tgacattgac
     9001 agtaatcaaa tgcacatcaa atggtgccaa ttctttaggg aaattgattc cccaagcgta
     9061 acggtattca ccttttggcg ttttgttaac aaagaggcga gcgtgttgct ccatcactgc
     9121 tgaaagaaga cggctgacac cgataccgta acatcccatg atgattggca cagcacgacc
     9181 attttcatcc aagacatctg ctcccatgct tgctgagtag cgagttccga gtttgaaaat
     9241 atgaccaatc tcaataccac gcgcaaagtt aaggacacct tgtccatctg gggaaatttc
     9301 accctcacga acttcacgga tatccacata ttctgcagta aaatcacggc ctgggttcac
     9361 accagtcaag tggtagccat cttcgttagc accgacaact gcattgcgaa catcttgcac
     9421 cttacgatct gcaataattt taatattctc tggcaaacca actggtccaa gtgaaccaaa
     9481 tcctgcttga acaacattcg ccacttcttc ttcgctagca acgtcaaaga aatctgctcc
     9541 taagtggttt ttcaacttga cttcgttgag ttggtcattt ccaactagaa gggctgcaac
     9601 aagctcacca tctgcaatgt agaagagggt tttaatcgtt tgttcttctg gaacattgag
     9661 gaaagctgca acttcatcaa ttgatttaac atctggcgtt gcaacacgag taacttcttc
     9721 ttcagcgaca acacggttgc ttggtttgta ctcgtttgtt gccatttcta agttagctgc
     9781 atagctagac tcacttgagt aagcaatggt atcttcacca gagactatcc atttgagcaa
     9841 ttctgccttg atttcttctt gcacttctgc aggaatttcg tcaaatgagg caactgactt
     9901 gtccaagaca acccagcggt caaggtctgt acgagcagat gtaatggcca taaattcttg
     9961 gctatcctta ccacccatgg ctccaccgtc accaataata gccttgaagt ctaaaccact
    10021 acgagtgaaa atacgctcat aggctgcttt gtactcatca taaacactat ccaaactatc
    10081 atagttagcg tggaaactat aagcatcctt catgataaat tcacgtgtac gaagaagtcc
    10141 attacgtggg cgtttttcat cacgatactt gggctgaatt tgataaaggt tgagtggcaa
    10201 ttgcttgtaa gatttaacag aatcacggac aatagctgta aaggtttcct cgtgagttgg
    10261 acctaagata aagtctgatt tttcacggtt ttttagtttg taaaggtctt caccataggt
    10321 ttcgtaacga cctgattcac gccacaattc tgcactaaga agggctggag ccaacatctc
    10381 aacagcacca atcttttcga attcttggcg catgatgttt ttagcttttt caatcacacg
    10441 gttggcaagt ggtagataag aataaacacc tgctgaaact tggcgaacat aaccagcacg
    10501 caacataaga gcatggctga taacttgagc atcgcttggc atttcgcgaa gcgttgggat
    10561 aggcatttta ctttgtttca taatattcct cgattatcta aaaaagagtc gcataatgtc
    10621 attccaagtc acagcaatca tcaagacaac catgatgacc actccggcca aggtgacata
    10681 ggtttcaatt tcttgtttca atggtttgcg gcggatggct tctaggatat tgagcacaat
    10741 cttaccacca tccaaggctg gaatcggaat aagattaaaa atcccaatat tgatggaaat
    10801 cattgccaag aagtacaaga tattttcaat tccattttta gcagcatcac tacttgcctt
    10861 aaagatagca acaggtccac ccaacttgtt caaatccggt tggaaaatca gatttttcag
    10921 agctgagaga attcggagag ctgagtcagc agcagttgta aaaccaccta caaacatgga
    10981 tagaaaatct gacttaaccc ccggttgaac acctagaagg taacgacctt gactatcttc
    11041 gggtgtaaca gtgacttgtt tgtcactccc cttttcagaa atagtcacat ccaaagtcgg
    11101 tgccgtctta tctttggttt ctgtttccac agcttggatc aagctttccc agttgctaac
    11161 ctcatgtgag ccaatcttgg taatttgtgc cgtttctggt actcctacct tggccaaggc
    11221 accttggggc atgatatgga actgattggt atcaacatct ctgacaccac cctgcataaa
    11281 gattaaaacc caaaaaacaa cgacacctaa gataaaattg ttcatagggc ctgcaaaatt
    11341 ggtaatcagt ttgccccaga tagtcgcatt ttgatattga acatctaaag gtgcaatccg
    11401 aacctcagta ccatctgctt ccacaaccgt tgcatcgtga tccactgcaa atgttttttc
    11461 ttcttccaga accaatcctt tgataaagag cttgtcttca aaatcaaact gggtcacctg
    11521 catagggagg gctgtttgat ccaatttttt acctgagaga ttgatgcgtt taaccttacc
    11581 atcatcagca agtgtcaaac taacaggcgt tcctgtcttg atttcagttg tatcatcacc
    11641 ccaaccggcc atgcggacat agccacccag aggcaagatt cgaatggtat aggccgttcc
    11701 atccttgcca atgtgagcaa aaattttagg tcccataccg atggcaaatt cacgtactaa
    11761 aatccctgat ttcttggcaa agtagaagtg cccgaactcg tgcaccacta caataatccc
    11821 gaaaaccaga ataaaggtta aaattccgag catagcgttt cctccgtctt ttgattaaaa
    11881 gagtccaaat aagtgcatga ttggaaatac aagcaacata ctatcgaaac gatccaaaac
    11941 accaccatgt ccagggataa atttcccaga atccttaaca ccaaaatgac gtttgatcga
    12001 actttctagt aaatcaccaa attgtccagc aatgctaaag aaaatagcaa agactgacat
    12061 cttgtaaatt ccatatggaa gagcaactgt actgtcaact atcataaaga taatggttac
    12121 taaaattgct cctaaaatac cacccaaggc accctcaagg gttttattag gcgataccct
    12181 tggtgctaac tttcgtttcc catagttcat cccaacaaga taggcaccac tgtctgtcgc
    12241 ccagacgata cacaaggcta agagagcctt gtccaaacct gcaacacgag catctagtaa
    12301 agcattaaat ccaaagccca cgtagaagct catagcaaga gggaaaaccg catcctcaat
    12361 cgtataagac ttgctaaaaa cggtcgttcc taacatgatt gaaatcaaaa cactataggc
    12421 aaccacattc ccatcaactg gcaaaaaagt caggtaattc tccaagggaa tggtcaatgc
    12481 aaaggttgca aagagggtca agaggccctc catcgtcatg gtctctagac ctctcatctt
    12541 caaaagttca tgcatggcta gcatggctat gattccgatt gctatctgaa gcaagaggcc
    12601 cccaatcatt aaaattggta ggaaaatagc cagggcaatc cctgcaaaca aggttctttt
    12661 ctgtaaatcc tgggtcatat ttcctcctaa actcctccaa atcggcgatg acgacgatta
    12721 taggcaagaa tagcttcctg caaggccgct tcgtcaaaat caggccataa ggtgtccgta
    12781 aaataaagct cactataggc tccctgccat ggaaggaaat tgctcaaacg caattctcca
    12841 ctagtacgga taatcaagtc tgggtctcgt aagtccttag gcaaatgctg ggtaaagaga
    12901 tagttaccaa tcaattcctc tgtgatgtca cctggattga ttttggcatc taaaacatcc
    12961 tgggaaatca acttaagcgc ctgtgtaatc tcagcacgtc caccatagtt aagagcaaaa
    13021 ttaagaatca atcctgtgtt gttcttagtc aattcctcag ccttggttaa agcttcaaag
    13081 gtttgcttag gcaggcggtc tgtctcccca atcatttgaa tcttaacatt attcgcatgt
    13141 agttccggga cataattatc ataaaactct actggcaagt tcatgataaa cttgacttcc
    13201 tgatctggac gggtccagtt ttccgtagaa aaagcataga ccgtaataac cttgacgccc
    13261 agtttgttgg ctgccttggt cacggtttgc aatgcttcca tgcccgcctt atgtccaaaa
    13321 actcgcggtt gcatacgttt tttagcccaa cggccattgc catccatgat gatgccgata
    13381 tgagcaggaa cctgtgtcgg aacctctact tccacagcct tatctttctt aaaaaatcca
    13441 aacatgatct tattcctatt caaaaatcta tcgttttatt ataccatatt tccccatttt
    13501 cttctatcac taagctattt attctcaggc accaagccca tttttcaaaa aaataagccg
    13561 cctgattggg cgactttatt tttataggga gattattatg aaaaagtttt aggagtttaa
    13621 gttaaggtct tcttaactta tgaacttagt gtacactccc tagcttaaag tttccttaag
    13681 tatttttaaa aatcaaattt ttccatttct cctgccaatt tttcttggat aaacgtgttt
    13741 gatagagttc cattcggtct tcattttcta agaaatgagg agttggacga acttgaaaat
    13801 tcaaaatatc ctccaaacca taaggtgcat agagttcaaa atctgattct tcattcaagc
    13861 gcagtccaac tgtcgtacac cgttctggat acttactcat agcatcacga gaactggtat
    13921 aggaagcagt gtgaggactg tgctgatgca tatagacctg atttttcaat tcccactggt
    13981 actgaggaaa atcctctcgc agctttttct ccagtaataa ggtttcctca taagaaaaat
    14041 ctggatcaaa gaaaatcaca tctatatctg tttcatgatc aaaaggggat ttgtctgaca
    14101 aaagattcca aatgaaattt ctgacagaac ctgctgccaa ccacgagtct ttcagaccaa
    14161 ggtctcggat gatcgtcaga atggccatca tatctggatt ttctctaaaa gcctctaaga
    14221 tttcttgctt atttttcact gtattcataa cctaagtgct catatgcctt agcagtcgcc
    14281 acccgtccag accgtgtccg catgataaaa cctttttgaa tcaagtaagg ctcatacata
    14341 tcttcaactg tctcacgctc ttcggcgata ttcacagaaa gagttcctag accaacaggt
    14401 cctccactgt acatctcaat catggtacga aggatttttt gatccacata gtccaaacct
    14461 tcatggtcaa catccagcat agtcaaagcc ttatcggtaa taatatcatc aattacccca
    14521 ttccccatta tctgggcaaa atcgcgcacg cgcttgagga gacgattggc aatacgaggg
    14581 gtcccacgac tacgtagggc caactcagat gctgcctcat gagtgatttc catctcaaaa
    14641 atatctgccg tccgctcgac aatttctgtc aagtcagcat gggcataata ctccatatgg
    14701 cctgtaatcc caaaacgtgc ccgtagcgga ttggagagca taccagcccg agtcgtcgca
    14761 ccaatcaagg taaaaggtgg taactccaaa tgaacactac gactaccctc accagcccca
    14821 atcataatat ctatgtagaa gtcctccata gcactataaa gcacctcttc cactgacatt
    14881 ggcaaacgat ggatctcatc aataaaaagt acatccccag gctctaactc attcaaaata
    14941 gctaccagat ctccggcttt ttcaatgact ggacccgacg tctgcttaag attgactccc
    15001 agttcgttgg caataacaaa ggccatggtc gttttcccga gacctggagg cccaaataag
    15061 agcacatgat ccagcgcttc atcccgcatt ttggcagctt caataaagat ttgtagctgg
    15121 tccttgacct tatcctgtcc gatatattca cgtaaatact gaggacggag cgtgcgttct
    15181 actaactcct catcccccat tatctcatta tctaaaattc tactcatggc tctattatat
    15241 caaaaaacca agccacaaac aaaaaagcca cctgattggg tgactcctaa gtttagcact
    15301 tatgtggtat aatattctac gacactgcta caccgcctac gaaaggaggt gagatagccc
    15361 atgatggaat tagtactcaa aactatcatc ggaccaattg tggtcggtgt cgttcttcgt
    15421 atagtcgata aatggctaaa caaggacaaa tagtgtcaaa aaagacctca agcttatttg
    15481 gtcgtgagct tggggtcttt tctagcctat gatatagaac tagtactcaa ttccttttta
    15541 ttatcccata gttcacgaat tttgtcaaag gtttacattt tcttcaaccg ctgtacgaca
    15601 agacggttaa gattaagaga acgttaggga ttctatcaat ttcatagaaa ttttgatttc
    15661 gtaaacgaag agacaatctt acatgtcact tctcatttaa tacgccacta ctagacaagc
    15721 aaaatcatta ttacagtagt tccagttctt caattaacag tcacttacaa tcaaattgag
    15781 tttgaactag ctgaagcgac cacagaccta tttcttagtt atattcgcta aaaaagtccc
    15841 cgccaattcc ccgaccaaaa tccgaaaaat accgaaaaat atcgaaaaat tatttttaga
    15901 atagtcccca aaagcctgaa atagagctaa aaaactccac ctgattgggt ggagttaagg
    15961 gagattatta tgaaaaagaa aagtttagga ttttattaaa taaagttagg aggtctttat
    16021 ttaatgacta tataatacta gaccatcctt aaattttgct taagaaaaaa caagttttgt
    16081 tattttttac gattcatcca agtcatccct atacaattat aggtggataa gatttcaaag
    16141 cccatagaac gatagaagcc caaggttctt tctgtctctt ctgtcaccag ctggacttga
    16201 taggcatctt tgtaatcctc taaagcctct ttcatcaagg cactaccaat tccttgacgc
    16261 tgatagattg gtaaaacgat taaatcctga accaatactg atgagaatcc atctccaacc
    16321 aaacgaatca agcccaccac agcatcacca tcaagtgcca gataaattac taatgaatga
    16381 gataaggcct gctccagcat ctctggttga tgggtataat ttgtccaacc gacagcctga
    16441 tagagatgta aaacatcctc tagcttgaca atttcttgct ttttaatagt aatcatctca
    16501 acacctctta aagttctctc aagctcttgt actgctgtcc atttttatca aaattttcag
    16561 gacgcaacca tgtttccaaa cgatctttga cttgaggcca gtccttatca atcatagaca
    16621 accaatccgt atctctcgta cgtcccttat aaaccactgc ctgacggaag gttccttcat
    16681 aaataaagct caaacgctcc gcagctcgtc tggatggcag atttaaggca tcgcatttcc
    16741 actcgtagcg acgatagttt agctcctcaa aaacatagcg agctaggaga tactgagctt
    16801 cagttcctat ccgtgtaccc cttagctctg gagaaaaagt gactgctccg acttctatta
    16861 ctcggttatt ctggtcaatg cgcatgagag aaaaagatcc caaagcctta ccagtcgcct
    16921 tgtctatgat tgcatagtaa aaacggtcct tacgagccaa catctgatgt aaaaggctaa
    16981 ccagctcccc catatctgct actggctcct gaaaaaggta ggtccacatc tcctgaggcg
    17041 tatcaggccc ataaacagct agcaaatcct ccgcatgctt ttccactgaa agagcctcta
    17101 tcctagcata acgcccttct aagaaatcaa tagaaggcag ttcacctggt gtataacctt
    17161 ccattgactc cccaatcatt tgaccatatt catttactgg catagctttc tccttttttc
    17221 acttttcaaa ctttatagtt gcccatagta taccatactt tcataagaag attcaaaaaa
    17281 tcctccagat tctactctga aggattccta ccttatttca caacgatatt aacgagttta
    17341 gttggctcaa acagtttggg aaactgtttg aggttggaaa tcagcaaata aattggttat
    17401 tttacaacaa tattaaccaa tttatttggt acactaatca ctttcacgat ttccttaccg
    17461 tcaatttctg ctttgacttt ttcatccgct agagcaattt cttgcaattc ttcacgtgat
    17521 aggtctttag cgaccatgag tttggcacgg actttacctt tgatttggac aacgatttcg
    17581 atttcgtctt caaccagttt actttcgtcc catgttggcc aagtcacgta agagattgac
    17641 tcacctgttg cagcaacagt ttgccagagt tcttctgcca agtgaggtgc aaatggggca
    17701 atcaattgga taaatccttt ggcatagtca acatagagct tgtcttcctt gttagccgca
    17761 ttgacaaaga ccataagttg ggcaatggct gtgttgaatt tgagggaatc aatttgctct
    17821 gtgacagctt taacagtttc gttgtaaacc ttgtcaagag cgccattgtt ttccgcaaca
    17881 atttccttac ttctaatcaa acggtaaaca cggtcaagga acttacgact tccttccaga
    17941 ccttcttctg accaagcaat cgaagcatcg agtggtccca tgaacatttc atagacacga
    18001 agggtatcag caccgtattg ttccaccaca tcgtctgggt tgacaacgtt cttgagcgat
    18061 ttagacatct tggctggcgc ttgctccaac tcttcccctg tttccacatg gaagaaagaa
    18121 ccatcacgtt tttcaacctt gtcagtcgcc acaagagccc cacggtggtc acgatagctt
    18181 gttcccaaaa tcattccttg gttaaagagt ttttggaatg gttccttagt tggaacaaca
    18241 ccgaggtcat agaggaattt atgccagaaa cgagcgtaaa gcaagtgaag tacagcatgt
    18301 tccgcaccac ccacgtagat atctactggc aaccattgtt tgagcagatc ctcgtcagcc
    18361 aatttctcag tattgtgtgg gtcaatatag cggaggtagt accagcttga acctgcccat
    18421 tgtggcatag tatttgtttc acgacgacct ttgacaccat cttcacgagt cacttcaagc
    18481 cagtctgtca agttagctag tggactttca ccagtacctg agggacggat atccttggtt
    18541 acaggcaaaa caagtggcaa ttcactttca gggacagctg ttgaagttcc atcttcccaa
    18601 tgaatgattg ggattggctc accccagtaa cgttgacggc taaagagcca gtcgcggaga
    18661 cggtaggtta ccttctcttg tccacagcct ttttcttcca accaagccac aatcttggca
    18721 atagcgtctt ctttgttgag tccatctagg aagtctgaat taacgtgaag gccatcttct
    18781 gtgtaggcag cttcttcaac atttccacct tcaagtactt cgacgattgg aaggtcaaat
    18841 tgtttggcaa attcccagtc acgttgatcg tgggcaggca cagccattac cgcacctgta
    18901 ccatagctag caagaacgta atccgcaatc cagattggaa tttccttacc attgacagga
    18961 ttgatggcat aagcaccagt ccatacacca gttttttctt tggcaaggtc ggtacgagcc
    19021 aagtctgact taaggctggc ttggtgtttg tagtctgcta ctgcttctgc ttgctctgtg
    19081 cttgtgatgg catctactaa ttcatgttca ggagccaaaa cagtgaaagt cgcaccgaaa
    19141 agtgtgtccg gacgagtagt aaagactgta aattccttgt ctgttccttt tactttgaaa
    19201 gttacattgg caccagttga tttaccaatc cagttgcgtt gcatatcctt gatagactct
    19261 gaccaatcta gttcatctaa gtcattgagc aagcgctctg cgtaagccgt gattttgagc
    19321 atccattggc gcattggttt gcggacaact ggatagcctc cacgttcaga agttccgtca
    19381 ggaagcactt cttcattggc aatggcagtt cccaattcct caacccagtt tactggcact
    19441 tcagcttcat aggccaagcc tttttcgtaa agcttggtga aaatccattg agtccacttg
    19501 tagtagtttg gatctgttgt gttgacttca cgatcccagt cataagaaaa tccaagcgca
    19561 ttgatttgac gtttgaagtt ggcaatgttt tccgctgtaa attctgctgg gtcattacca
    19621 gtatccatag cgtattgctc tgcaggcaaa ccaaaagcat cccaacccat tgggtgaagg
    19681 acattgtagc cttgcgcacg tttgtaacga ctgaggatat cggttgcagt ataaccttct
    19741 gggtgtccta cgtgcagacc agctccagac ggataaggga acatatcaag cgcataaaac
    19801 ttaggttttg atgcatctgt tcctgtctta aatgtatgat gttctgccca gtagccctgc
    19861 cacttaggct caatttcttt atgattgtaa aaactcatgg tctctctcca attttgtgat
    19921 gttactatta taccattttt gagggaattt gaaatgtgtt taaaggaaaa actgtatact
    19981 gtatctcatt aattttgatg actatgaatt gctctatctc ccacttttta tcccattgtt
    20041 atgcagtaaa tcaaggagta agtcaagaaa cttatctcgt atagcacaaa atacttattt
    20101 tagaccgaag ataaaactgg aatgctaatt aatgtattca caataaatag ccgacaacct
    20161 atgtcgcttt ttgatttatg aagaacgtat agtggactga atctgaaata gtacgaaaca
    20221 attgctaaaa tacttataga aattaatttt actttcccaa tcgatttgtt ctcatcttat
    20281 ttcaatctgc tatagaattc aatacgagga tcaataaaag actaccattt ggaaaacagt
    20341 agtccttatt gtttattttg aatttacata ggcatctaca gcgataatag cttgagcaac
    20401 atctgaaggc gaaatcttaa acggcatctg atgaattgtc tcaccctcca tagttgcttg
    20461 tttaccaact tttattaaat catcatatcc aacttgatcc aaatgcattt cttttagagt
    20521 tgttggcata ccaatttttt tgtaaaactc aatatactta tcaagttctt ctttaggtct
    20581 attttccaat aatagttgta ctaaagttcc ataagctact ttttcaccat gtgttaaatg
    20641 atgaatgtca cctgtcaatg cagtaaaacc attatgaatt gcatgcgccg cagctaatcc
    20701 tccactttca aaacctaggc cactcaataa agtattagct tcaacaatat tttctaatgc
    20761 tggtgtcacc actttagctt cacaagctgc catagcctgt aaaccatctg caaacagcgt
    20821 ttcttcacat ttcttcgcaa ttgcaactcc agccaatgtt tgctgttgtc ccaacatagt
    20881 ttttccattt gcctgcataa ccgcacgcgc ctcaacccaa gttgctaaac catctgcaat
    20941 accagacgct aataaacgct taggggcttg tgaaataact tttgtatcaa ccaaaactaa
    21001 atctggattt ttagaataaa atagataatg atcaaatgca ccttcatctg tataaataac
    21061 agataaagca gatacaggtg cgtcggtcga tgcaattgtt ggagcaataa taacaggctt
    21121 ttcaatcaaa tctgcaatag cttttgcgct atcaatcgtc tttcccccac caagaccgat
    21181 aatactatca caattttctt tctcagccaa ggcaacaact cgattgattt cattgtcaga
    21241 agcttcacca ttaaatagcg ccagaacaat atggaaacca tacctatgta gataatcttc
    21301 aaatcgtttt ccaacaatat cataaaccaa ctgatcgcat aatagaatag ggtaatttcc
    21361 caaatccaaa attgatttgg cattttcaaa caaggcattt tccccctgaa tatatctaga
    21421 aggactagca aaaattctca tatgtatttc ctctctatct aaatggaacg actattttga
    21481 gtaacaaacc aatcgtcaga aaaatcatca accgcctttt ggatagatgg catggcgaaa
    21541 gctgattcaa aaacatctgc tcctgctgta acagcatgcg cacctgcagc taaagcatta
    21601 tttacttgtg ctacattttt aaaggatgca gctaaaatct tactaggaga gttctgtcta
    21661 tcaatagcaa gagctaattg acgaatgaca gaatttgaat caatgttcag attttccatt
    21721 ctattataat atggagctag gtaatccgct cctacttcga tagctaataa tccctgaata
    21781 actgtataaa tagctgttgc agtgatatgg tagccctctt tttttagcac ctttattgca
    21841 cgtaatccag ctggagtaac aggtactttg ataaatatat catctcctgc ttgtcttcga
    21901 atttcatgag catcctttaa gatgccttca aaatcttgag aaatcacctg aacatgaata
    21961 gagggtgtag agccaatcaa ttctcttaca tctttgattc gttcaaaaaa attaatagaa
    22021 ccctctcttt ttgcaatagt gggatttgaa gttaccccag ctagcggcaa aatttcagac
    22081 cactttttaa tctcatctaa atttaatgtg tcaagcataa attccataaa aagaacctct
    22141 ttattttaca aagtatgctc agtacgtcct ataatgtcat cttgggttgc cttagaaaga
    22201 acattgaaga atgcagagta tcctgcaaca cgaacaatta agtctctgtg tttttcagga
    22261 tgtttctgag cgtcaatcaa cgtctctctg gaaacaacat tgtattgaat atggtaccca
    22321 tgtaaacgat taaagaatgt tcgtaacaaa gcaattagtt ttaatttatc ttcttctttg
    22381 gctaacgttt gaggatttac tttctgattt aagagaaccc cacctacgat ttcatctgtt
    22441 ggtaattttg aaacagattt taaaacagat gtagggccgt gttgatccat attatgtgat
    22501 ggtgaacaac cctctgctaa cggtgtaccc gcgttgcgtc catctggagt tgctaatgtt
    22561 ccacgtccct gccctacgtt ggctgagata gaagatgttc ctgaataacg aattcctcca
    22621 ataggccctc ttccataacg tgtattagga tatttagcaa tttcatcaac ataaatgtca
    22681 taagcagcag taaccaattt gtcagcataa tcatcatcat taccatactt aggtgcatca
    22741 tgaatcaaca tttcttgaat gaccttacct tcttctccgg cataatctgt ttccagtgca
    22801 tgccaaagct gacttgggct tatacgttct tcctcaaaca ccaatttttt aattgcagct
    22861 aatgaatccg acaaatttgc aattccaact tgcaatcctg atatataatc atatactgct
    22921 ccaccttctt taaggtgttt tccacgacca atacaatcat cagtcaatgc tgaacataga
    22981 atatcaggaa cttctcgttc caatgataaa tcaatagaat tttcaacaat aacactcatt
    23041 cgtgtcaaat atcttagtgt tttatcccaa gcattttcta attcagaaaa gttcttcata
    23101 tccttaaaat gaccaaagct tggtgcaaac cgtttacccg aagccggatc aattccatca
    23161 ttcatcgtga taagtagaac cttagggaag ttcatataac tcatacctgt gcaacgatag
    23221 ccccatttcc ctggaactgc cgtttcaaca catccaatgg cactgtaatc ataagcatca
    23281 tcttccaata ctccttttgc aataaaagaa ggaataataa tctcatcatt attaaatgca
    23341 ggcataccaa aaccaagttt catcacttca atacactcat tcatgaaacg agcatctaaa
    23401 cctgcatggt aacgtacagt tagattaggt tgcggtagat gggtttgtgc aactgatttt
    23461 aataccaaat aagataatgg gttaacagca tccttcttat ctcgagtctg tccaccaatt
    23521 gtaacatttt gatataaagg acttcctgct gaagaaaatg tatgtgattg actgcgaacc
    23581 ttattgattg taattgtctt aatccaaaga tttgtcagac gttcaacaat gctatcttct
    23641 gtttctttac cactttctaa atcagccttc atatatggat acatatattg atcaaaacgg
    23701 ccatatgaaa gagagtggcc attagattca atttgtaaaa tacattgaat aaaccaaact
    23761 gattgaatag cttctgcaaa agtagttgcc ggttcatatg ggactctaga gcaaatatct
    23821 gcaatctcaa gtaattcttt cttacgttta ggatttgcat tttcggctaa acttttagca
    23881 agagcaacaa agcgctttgc atatacttta atagcatcga ttacgataaa tatagagtcg
    23941 taaaaatgat atttatcaat acttgctgga tctgttaaat ctagagctac ttttgcttta
    24001 cgagcccgct cttcaaaacc tcttaaacca aattgcaaaa gtttctgata gttaactgct
    24061 aggtgagcat ctccagaatt catcttacct tccataccga agaatcctgt ttccatataa
    24121 acagacactt cttcaggtaa taaggcacca gctctagcac gtaaattatt attttcccaa
    24181 aacggagcaa tacttctaag ttgttctttt gtttcttctg taatatagaa aacatctcca
    24241 tcacgctttt caaaaagatc caactcattg agaacaaatt ctagcgtata ttccggaaaa
    24301 ataggagcat ctttattgga agaagcttga tttcccgcaa tcatagattc ttcttcaata
    24361 tagatagtca tattttccaa aatttctttc agcatatatg cacgttttag gacattaggt
    24421 tgttccttgt atcgttcata ggcgcgtgtt gctagaactg ctctctcagc atcaatataa
    24481 ggttttttat ttaaaacatc ttctcgatat ttgttcatcc tttcagtaag gctgccaaaa
    24541 tattctgtct tgattgttgt tcttgctact tctgtattta ccatcgtaac cacctttctt
    24601 tcgaaaatat ttttatttct ttcgaaaata taataccgat ttttatagaa attttcaagc
    24661 ttttaactag aaaaatttta actctctaat ttttagcaca aatgtgtgag tttaacagta
    24721 acacacaaat acctctaaga ttgtttgatt tcattttggt aagctaaacg atcctgtact
    24781 ttaaagaatg gaaaataaac caatgtagac atcgctaata tcaccagctg agtaataact
    24841 ccttgccatc cacccaccaa aaatcctgat aaaatagctg gtgtactcca aggcaatgtt
    24901 acccctgaga atggctgcat gaaacctgtt gcaatagctc catatactat cacagctgca
    24961 agtacaggaa caagaatgaa aggtacaaac ataactggat tcatgacaat cggaaatcca
    25021 aatacaactg gctcatttac gttaaatatt gctggaaaag ctgcaacttt tcctaaggtt
    25081 tggtattgtt ttgattttgc tgcaaaaagc atggcaacta caagaccaaa cgtaatccct
    25141 gaacctgata gaattaaaaa tgaatctaaa aattgttgag taacaatatg tgcaccattt
    25201 tctaatgata gattagcaga ggctaacata gctttattag cgtcaagatt agataaaagc
    25261 agagctgtca ctactccatt tactaccgat tgcccatgaa caccaaacca ccacaaaaat
    25321 gatataaaga atgcaattcc aatagcacca tacaaagatc cagttaaacc ttgcaacgga
    25381 acttgaatag cagaataaat catttctatg aatgttccgc cattagtcaa tgacttcgct
    25441 aaaatatata caatcataga agataagaaa attacaaatg ctggaatcat tgcttcaaac
    25501 tgtttggcaa tagcttgtgg aacttgttct ggcatcttaa taacaatttt tctctttata
    25561 aagaaggtat aaatacttcc tactaccaaa cctataatga tagcaccgat aattccttgg
    25621 cctccaaacc aaactttact aatagcgtcc ccaatcgcct caccttgttt agggatataa
    25681 gatgatctta gcaaaataaa gaatgcagat acagatagaa ctccagctgg taaagcctct
    25741 actccgctat tcttagcata agaataggca attgaaaaac aagaaattag acccataata
    25801 gcaaaagttc ctgaatatac ttgcataaac ggctctgtcc aattagctcc aaaaacacta
    25861 gcaatgctct tatttaatcc ttcgaacggc aattgtccca taatcaagaa caaactacca
    25921 actactgtca atggcaaaat tgctaacatc ccatctttta gagctataat gccacgcata
    25981 ttcacaaact tcatcatcgg tgcaatgatt ttctgaacat ccatctttga cataataaat
    26041 ctccttttct tacccactaa tcaaagatag ggccaaatct aatacttttt tcccatctaa
    26101 cataccatag tccatcatcg gaataacagc tatcggaaca tcacacttat cacaaatttc
    26161 ttttgattta tctaatgtat aagcaacttg tggacccaat agtgcaacat ctatatttgg
    26221 cgcataatcc gctaatttag actgagaaaa cgcctctatt tctgcctcaa ctccactagc
    26281 ttgcgctgca attttcatat tatttacaag cataccagta gaaaaacctg ctgcacaaaa
    26341 caaaccaatc ttcaccatta tgttttcctc ctctatgtta ataacaatga taatactcta
    26401 gtattatttt ttatgaagtt cttttctcaa actaataatt tctttgatta aattaatctc
    26461 cgtcatacta gtcatgagat gatcttgtga atgaacaaag agagctgtaa tttctgtctt
    26521 tgtaccagac gcttcctgtg ccaagaattt tgtttgtaga ttatgagctt ctaataaggc
    26581 ggcatcagct aactggattt cttcttcaca tcgttcacat gtaccgttct ttatgtaatc
    26641 taatgcttta taaatatgtt gtttcgcatc gccagcatat aaaattaaac ccatgataat
    26701 ttgatctgga acaataattt ccataaattc ctcctcattt cactatgtaa aaagcttttt
    26761 tcatttgaaa ttttaaaatt ttcttttgta actatagaat acaactacat aataaagttg
    26821 tcaatccaaa aacaacagat aaactatttt tgtttatata agcacaaatg tgcaatcgac
    26881 taaaaagaaa aaacgataaa ctgatcagtc tatcattttc actcacctag aatctctcta
    26941 aaagtcaaat caccatcttc ttccaaaact tttaaaattg tatttttatc tgtaatcaaa
    27001 tgatttacta aattagcacg caagacagaa agaatcgaag atactttcgt atcaccgtaa
    27061 gcaacagcta aactttgagg aatatttttt aaatcttcca aagagatggc tatcgttctt
    27121 tcctgtaagt tttcatagac ttctttacct tttgaatcaa aaaatcgaca acaaatttct
    27181 cctacagttt taactttggt caattcttta aagtcatcct ctgtaagcat gtctaaccat
    27241 tgatgtttcc ctttattgct aaaatcacca attccgacta cagctatatc taaatctttc
    27301 caactatttt tcaaattttc aaaatatctt gattgcaaaa taccatctgc taacaattta
    27361 ttttcttgca caatcgttgc attcataaat gtacactctc catgaaattt tctagacatt
    27421 tcataaatca gtgtattcac atggtattta gcgtgtatgt gactaggacc acctgctaga
    27481 ggatagaagt gaacatttcg gacactttta ctgtgaatta aatctactaa attacttaaa
    27541 cttttccccc aagaaaagcc aattttcata ttatcatcaa ttagattcct aaggacgcct
    27601 gctgcaactt gagaaattct ttcagataaa attgttggag tatcatcaaa ttcatttgga
    27661 ataatttcta aactttccaa actgtatttt tcttttacat aattttccaa cttaaacata
    27721 ttggtatcaa aattctctat ttcaatttta acaattccta cattccttgc ttctgttaac
    27781 attctactaa tagaggttct ataaattcct aattttgctg ctatttgtga ctgatttaag
    27841 ttttcaatat aatacagata agcaatttta gaaagcagtt tattcctatc ttgattcata
    27901 cacttaacct cttacgaaac taccttaacc attatcccag cattttctaa tgtagctata
    27961 ttttgtttag aaagtttttc gtctgttatt acttcataga cttgacttaa agcaaatctt
    28021 cttactgtac ctcttttatc aaatttactt gagtcagtta ggacaatgac tttatccgac
    28081 actgctgaaa tatattgaac tacctcactg cgcattaaat cttttccggt aaagcccatc
    28141 tctttatcgt aaccatctgt cccaacaaaa gcttgacaca catgaaaagt ctgtatcatt
    28201 tcttttaata aaggtcctac agtcacctgt gaatctttct gaaactcacc accaagaaca
    28261 ataacacgac atgaatcata agctctcaca aaatttgcta taaaaaacga atttgttaca
    28321 atcgtaacat ttcttttttg cttgcaaatt tcctcagcaa gtaaagcaca ggtcgatcca
    28381 gattctatca ttattgtttc attatctgac accaatttta ctgcttcctg aacaattttt
    28441 ctcttagttt cataattaat tgacaaacgt acatttaagt catctccact atttaataca
    28501 gcatatccat gctctctgtg taataaacct tttgactcta atttatctaa atcttttcta
    28561 atcgttactt tcgatacatt taatttttcc gataatgtat taacgtcgat cttttcatat
    28621 tctgatacta atttaataat ttgttccaat cttttcattt tacacctccg ttttattcta
    28681 ccaaaataaa aagcaaaaaa caacaaatta aactttcgtt cgtaattgtt tttctttcgt
    28741 ttttgtgata ggatagactt atgaagagga ggaactctta tggaaatatc taaaggaatt
    28801 atttttaata ttcaacactt ttcaattcat gacggcccgg gtattcgtac aactgttttt
    28861 ttaaaaggat gtcctctgcg ctgtccatgg tgttctaacc ctgaatctca aagaatgaaa
    28921 cctgaaaaaa tgaaagatgc tcaacgagag aaattcacct tagtcggtga agaaaagact
    28981 gtagaagaaa ttattacaga ggtattaaaa gacaaagaat tttacgaaga atccggtgga
    29041 ggtttaactt tatcaggagg tgaaatattt gctcagtttg aatttgctaa agccatctta
    29101 aaatcagcta aagaacatca catacacact gccattgaaa ctactgcctt tgttgatcat
    29161 gaaaaattta tcgatttaat tcaatatgtg gattttatct acacagacct aaaacattat
    29221 aattctataa aacataaaaa agtgactggg gtttttaatc aaatgattat taaaaacatt
    29281 cattatgctt tttctcaaaa taaaactatc gttttaagaa tcccagttat tcctaatttt
    29341 aacaatagtt tagaggatgc agaaaaattc gctactctat ttaactcatt aaatatcgac
    29401 caagttcaac tactcccttt tcatcaattt ggtgaaaaca aatatcgttt attaaatcgg
    29461 aaatatgaaa tggatggagt caacgcactt catcctgaag atcttattga ttatcaaaag
    29521 gtatttctga accaccatat taattgttat ttctagttca tttccttgaa acgctctagc
    29581 tatttgcaga taacaagcat ctatataagg ttatcaaaga agacttcccc atgactctaa
    29641 atgaatatat cttacaatat cgcctgaaac aagctataga taagatggct gaatctccca
    29701 actcctcttt aagcgatatc tctgaccaag ttggattttc agactataaa tattttgcca
    29761 aagtatttaa aaagtatctc catatttccc caaaaaaatt gaaatcactc ggaagaatag
    29821 taaaataggc tattcttctt tttgtagaga attttactct aatatagtga attgaaataa
    29881 gatgtgaaca tctctatcag gaaagtcaaa ttaatttata gaaatatttt agcagtcaag
    29941 gtgtactatt atagattcaa tgcactatag aatagaattg caggttttaa aaacgcttac
    30001 attgttttaa aataaactta aataacacaa cggaggtatc catcatgaaa aagaaatgga
    30061 tgtattatgc tgcttgttct tctaatgaat ctgccgatga cagttcatct gataaaggag
    30121 acggtggttc gctagtcgtt tattgaccaa actcagaagg cttaattgga gcaactattc
    30181 ctgcctttga agaaaaatat ggtatcaaaa tagaactgat tcaagctggt actggagaac
    30241 ttttcaaaaa actagagtca gaaaaagaag ttcctgtagc tgatgttatc tttggtggtt
    30301 cttatacaca atatgctacc cacggagaac tctttgaaaa ctatatttca aaagaaaatg
    30361 ataatgttat caaagaatat caaaacacaa ctggcttctc tactccttat acactagatg
    30421 gtagtgtttt aatcgtccac cctgatttaa ctaaaggcat gaacatcgaa ggatatagcg
    30481 accttctcaa acctgaacta aaaggaaaaa tcgcaactgc tgacccagca aactcttcta
    30541 gcgcctttgc tcaattaaca aatatgctac aagctcaagg tggttacaaa gatgataagg
    30601 cttggtctta tgtaaaagat cttttcacac ttattgatgg tatagtaaaa tgaaataaga
    30661 acaggacaag ttaatcagga cagtcaaatc gatttctaac aatgttttag aagtagatgt
    30721 gtactattct agtttcaata tactataaaa tcggtttaag ttcatctggt gtctataaag
    30781 cagtcgctga tggagaaatg actgttggtc tctcttataa agatccagca gttaaactct
    30841 taaatgacgg agctaacatt aaggtagtct atccaaaaga aggaaccgtc ttcctacctg
    30901 ctagtgctgc tatcgttaaa aaatctaaaa atatggaaaa tgccaagaaa tttatcgatt
    30961 ttattatctc tcaagaagta caagatacac ttggtacaac cactactaac cgtcctgttc
    31021 gtaaaaatgc taaaacaagc gaaaacatga aaccaattga caaaatcaaa acactcactg
    31081 aagattatga ttatgtcatc aagaataaat cagatatcgt taagaaatac aacgaagtct
    31141 ttacagatat ccaatctaaa cagtaaaaga ggttcactat gagtgagatc aaaattatta
    31201 acgccaaaaa aatctaccac gatgtccctg ttattgagaa tttgaacatt acaattccaa
    31261 aagaaagtct ctttaccctt cttggacctt caggatgtgg gaaaacaacc cttcttcgta
    31321 tgattgcagg tttcaacagt atcgaaggtg gagaatttta cttcgatgat acaaaaatca
    31381 ataatatgga acccagcaaa cgcaatatcg ggatggtttt ccaaaactac gctattttcc
    31441 cacatttgac tgtccgagac aacgttgctt ttggtcttat gcaaaagaag gttccaaaag
    31501 aagaattgat tcaacagacc aacaagtatc ttgaactcat gcaaattgct caatatgcgg
    31561 atcgaaagcc cgataaactc agtggtggac aacaacaacg tgtcaccttg gcatgcgcct
    31621 tagcggttaa tccaagtgtt ctcctcatgg acgagccact tagtaatctg gaggccaaac
    31681 ttcgcttgga tatacgtcaa gccatccgag aaatccaaca cgaagtggga attacaactg
    31741 tttatgtaac ccacgaccaa gaagaagcca tggctatttc agaccaaatt gctgttatga
    31801 aagatggggt gatccaacaa atcggccgac caaaagaact ctatcataaa ccagctaatg
    31861 agtttgtggc aacctttatc ggacgcacaa atattatccc tgccaatctt gaaaaacgga
    31921 gcgacggcgc ttatatcgtc ttttcagatg gctatgccct tcgaatgcta gctcttgatc
    31981 aggttgagga gcaagctatt catgtaagca ttcgtcccga agagtttatc aaagatgaat
    32041 ctggagatat tgaaggaact attagcgata gcgtctatct tggactaaat acggagtatt
    32101 tcattgagac aggttttgcc tcaaaaattc aagttagtga agaatcaact tttgaagaag
    32161 aactacaaaa aggcaatcgt attcgtctac gaatcaatac gcaaaaatta aacatctttt
    32221 ctgcagatgg ttcccaaaac ctgataaaag gagtcaacca tggaacgtaa aaaactaaat
    32281 atttggacag cctcctcttt cttcatcttt cttacctatc ttgtctttct cgtttatcct
    32341 atcgttaccg tgctcaagta agcacttata catgaaggat aattctcact agctaatttt
    32401 gtcactttct ttagtaaaac ctactactct gagacactag tcaacagttt cagggtttcc
    32461 attaccgcta ctgtcacttc cttagttgta ggaaccctat tagcttatct cttctctatg
    32521 tatgacttca aggggaagaa atttctacaa atattgatta tcattgcttc catgtcagct
    32581 cctttcgtag gagcctactc ctggattctc ttgctgagac gaaatgaggt catcactaaa
    32641 tttttgacaa atgcccttca tcttccagct atcgatattt atggattcaa aggaattata
    32701 cttgtcttta cactgcaact attcccactg gtatttctat atgttgctgg aacaatgaac
    32761 agtattgaca attctctact tgaagctgct gaaagcatgg gatccttcgg atttaaacct
    32821 atcgtaacgg ttgttttacc tctcctagtt ccaaccttac tagcagcccc ttgcttgtat
    32881 ttatgagagc attctcagac tttggaacgc ctatgttgat tggcgaagga tatcggactt
    32941 tccctgtcct gatttatacc caatttatta gcgaggttgg aggaaattct gcttttgcaa
    33001 ttatggcgat tatcattgcc ttggcaattt tccttatcca aaaacacatt gcaaaccgct
    33061 acagtttcag catgaatccg ctccatccaa ttgagcctaa aaaaactaca aaaggaaaaa
    33121 tggctgccat ttatgcaaca gtctacggaa ttatctttat ctctgtttta cctcaaatct
    33181 acttaattta tacctctttc ctaaaaacat caggtatggt atttgttaaa ggttattctc
    33241 caaacagtta caaggtagct ttcaatcgta tgggatctgc tattttcaat accattcgta
    33301 tccctttgat tgccttagtt ctagttgttc tatttgcgac atttatctcc tacctagccg
    33361 ttagaaaacg gaatttattt acaaacttaa ttgacagcct cagtatggta ccttatattg
    33421 taccaggaac cgttctaggg attgccttca tttcttcctt caatactggt ctatttggaa
    33481 gtggatttct tatgattaca gggactgctt tcatcttgat tatgtctcta tctgtcagaa
    33541 gattaccgta tactattcgc tcatctgttg ctagcttaca acaaatagca ccaagtattg
    33601 aagaagctgc tgaaagctta ggaagtagtc gtctcaatac ctttgctaag attacaactc
    33661 caatgatgct atcgggtatt atttctggag ccatcctatc ttgggtcaca ctgatttcag
    33721 aactctctac ttctatcctc tatcctcctc tacaatgtca aaacaagaac aatgactgta
    33781 gctatttata cagaggttct cagaggaaat tacggtgtag ccgcagcctt gtcaactatc
    33841 ctgactgttc taacagtagg ttccttgctc ttgtttatga aaatctctaa aagcaatagc
    33901 attacacttt agttttccac atcaaaaaca gccgaaagga gtaccctcgg ctgttttttc
    33961 ttatcttgat attcttatca aatccctagc tcatggcaag caagtctaga ctgattaaat
    34021 agagccttct tccatcttct cacgtcctag ctctcggtaa ctacggtcac cgacaacttc
    34081 aaccctaaac tgaccgtcct catattcaag aatcgtcacg ctaccattat ctagaccatg
    34141 cggatgcatg ccattaatca gataaacaat ggttccaata gtcattccat ggctgacaac
    34201 aagggcgtta cctccacctt gatcttccat ttcttttgca attatttcaa agccttcctt
    34261 gattcggcca ctgagttttt cccaaccttc agcccaacca gctgtatcga cctctaccaa
    34321 gccctcagcc agttcagcat aagacaattg gtgaacgtgg tccacattaa agatacgagg
    34381 aataatgccc atgaaaagat cgccatcata ggctccatca aaactaccga aacaccattc
    34441 tctgatacgc ttgtccatgc gataagggat tttcccctgc aagccaagtt cttcaagtat
    34501 aattcccatg gtctgaatgg tacgaccaga atcactcgaa taagcgcgct caaactgtag
    34561 atcagattct cgcaaaccga ttcctaactc ttgaatccct cgttcacctt cagcagttaa
    34621 gggagtatcg ctccaacctt gcgcgcgacc aatcgtgtta aacatggtct tgccatgacg
    34681 taccaaatac aatcgtactt ttaccatttt ccgtcctccg tttgcttcta ttatatcatg
    34741 aatgataaaa taaaagccac ccatacaggc gacttttgaa ggagattatt atgaaaaagt
    34801 ttaggagttc attaaataaa gatattagat gaaaatcaaa ttcaaactaa ttcagtgtta
    34861 tctgtttcaa ataatattag gaggtcttta tttaatgatt atagtataca catctaatct
    34921 taaatagaac ttaaaatttc tcctattttc ttaattttta aaactatgaa ttggtgctgg
    34981 gatttgtcca ccgcgagaga tgaagtcgac agacgaagcc ccattaacct tcataacggg
    35041 tgcagttcct aatagaccac caaactcaat catatcgcct tctcttcctt tgggaatgat
    35101 acgaacagct gttgttttca tgttgataac accgattgct gcttcatccg caatcatagc
    35161 cgcaatagtt tcagcaggcg tatcttctgg gatggcaatc atatccaatc caacagaaca
    35221 gatagccgtc atagcttcta gtttttctaa attaagagag ccattttgca ctgcagcaat
    35281 cattccttca tcctcagaaa cagggataaa ggcaccagat agaccaccga cctggttaca
    35341 ggccatcact ccaccctttt taacttggtc gttcaagagg gccaaggcag ctgtcgttcc
    35401 atgcgtgcca actgtttcta gccccatttc ctcaaggaca cgtgccacag agtctccaac
    35461 cgcaggggtt ggtgccaaac tcaagtccac aataccaaac tccacaccca gtctctcact
    35521 ggccatttga ccaaccaatt gaccgatacg agtgatttta aaggcagttt tcttaactgt
    35581 ttcggctact acatcaaagc tctgtccacg aactttttcc aaagcacgtt tcacaacacc
    35641 aggaccagaa actccgacat tgatgataac atctgcttcc ccaacaccat gaaaggcacc
    35701 cgccataaat ggattgtcct caacagcatt agcgaataca accaacttgg ccactcccat
    35761 atctgaaaga tttgctgttt ccttgataat tcgtcccata tctgccacag ccgtcatatt
    35821 aataccagac ttggttgagc cgatattgac tgacgagcag accttatccg tctcagccaa
    35881 agcgcgagga atggaattga tgagaatctc atctcccttt tgataacctt tttgtactaa
    35941 ggcagaaaaa ccaccaataa agtccacacc aatctctttc gcagccttat caagcgcttt
    36001 tgccagaacc acgtagtccg tcgcatctgt cgctgcccca atcagagaaa taggtgtcac
    36061 cgatacacgc ttattaacga taggaattcc caactcagcc gcaatttcat caccaacagc
    36121 tactaaatta gccgcctttg tcgtaatttt ttgatagatt ttctccgcag cacaattgat
    36181 atctggatcg atacagtcca aaagagaaat ccccatggta atggttctaa tatcgaagtt
    36241 ttgctcctca atcatggcga tggtttcagt aacttgtcta atatccatga tgacctccta
    36301 gatattatac atagcttcga aaatcgctgc actctgaata ttgattttta cattcaaagt
    36361 ttgcccaaaa gcttcaaatt cattacgaag ataggtaaaa tcttgctttt catcactaga
    36421 tacaacagcc atcatcgtaa aatattcatc taagacagtt tgagagatat cgtcaatatt
    36481 caatcctaat tctgcaattt taccagaaac acctgcaaca attccagatt tatctttacc
    36541 aacaacagtt ataatggctt tcataagtaa actccaattc ttcttaaaat aagaaacctt
    36601 aacattaaac aggattcttt tcaaatagta tagcataatt ctaacgaaaa tactcaaaaa
    36661 cgcctgctta cgaagttgtt cttaagaaaa aaacaatttc gtaaacaagc gtctactatc
    36721 ccaacttatg atgagtgttc ccgctaggac cgaaatccta gcggtagacc agagctagac
    36781 taagagcaca agactccatc atcataacac tcaacaaaat tgatgatttt atactaattc
    36841 gatgatcgcc attggcgctg catcaccacg acgtggttca gttttaagga tacgagtgta
    36901 tccaccgtta cgttcagcat aacgaggtgc gatttctgag aacaattttt gaagtgctgt
    36961 agtagaagtg tacttatcag ttgcttcatc atagttttca gatgcgattt cattacgtac
    37021 gaaagcagct gcttgacgac gtgcatgcaa atcaccacgt ttacctagag taatcatttt
    37081 ttcaacagtt ttacggattt ctttagcacg agcttcagtt gtcacgattg attcgttgat
    37141 caaaaggtca gttgtcaaat cgcgaagcat tgctttacgt tgtgagctag tgcgtcctag
    37201 tttacggtaa gccatgtatt cctcctttat ttatctttta atccaagacc caaatcaatg
    37261 agtttgagtt tcacttcttc caaactcttg cgtccaagat ttcgtacttt catcatctct
    37321 gcttcagatt tttctgtcaa atcatgcaca gtattgatac cggcacgttt taaacagttg
    37381 tatgaacgca cagacaagtc cagttcctca atcgtacgat ctaaaatacg gtcgtcagat
    37441 tcagtatcag cttctttcat cacttcagtt gacttagcaa tctcagtaag atttgtaaac
    37501 aaatcaagat gttctgtcaa aatacgtgct gaaagcccta aagcatcttc tggaataatt
    37561 gttccatttg tcaagatttc aagggttaat ttgtcgaaac catcattgct acctacacga
    37621 gcaggttcca cttgatagtt gacttttgta actggtgtat aaatagaatc tacagcaagt
    37681 gttccaactg gtgcattatc ctttttattt tcatcagcag gtacatatcc acgaccactg
    37741 ttaacagtca tagtcgcttt tagagaagaa ccttcaccga ttgtaaagag ataatgatct
    37801 ggatttacaa tttcaatatc gctatctgtc aaaatgtcac cagctgttac ttcagcagga
    37861 ccttcaacat ccagttcgat gattttttcg tcttcaacgt acgatttcac tgcaattcct
    37921 ttaatgttca gaatgatttg catcacgtct tcacgaacac ctggaactgt gtcaaactca
    37981 tgtaacacac catcaatgtt gatagatgtc acagctgctc ctggtagaga agctagaagt
    38041 acacgacgaa gagagttacc aagagttgta ccgtagccac gttcaagtgg ttcgattaca
    38101 aacttgccat aatctttatt ttcatcaatt tttgttatat ttggtttttc aaactcgatc
    38161 atttagttac tccctcttaa acgaaaagca gtgtaatgcg atgattatac acggcgacgt
    38221 tttggaggac gagcaccatt gtgtggcact ggagtcacat cacgaattgc tgttacttca
    38281 agaccagcgg cagcaagcgc acgaatagct gactcacgac cagaacctgg accttttaca
    38341 gtaacttcaa ctgatttaag accgtgttct tgtgcagatt tagcagcagc ttcagaagcc
    38401 atttgagcag cgaatggtgt agatttacga gaacctttga aaccaagagc accagctgat
    38461 gaccaagcaa ttgcattacc atgcacatca gtaatcataa caatagtgtt attaaatgta
    38521 gcgtgaatat gagcaatacc agattcgata ttctttttca cacgacgttt acgtgttggt
    38581 ttagccaaga cttttacctc ctatattatt ttttcttacc agcaatcgca acagctttac
    38641 ctttacgagt gcgggcgttg tttttagtgt tttgtccacg gacaggaagt ccacgacggt
    38701 gacggatacc acggtatgaa ccgatttcca tcaaacgttt gatgttcaag tttacttcac
    38761 gacgaaggtc accttcaact ttgattgcat ccacttcacg acggatagca tcttcttgat
    38821 ctgatgtaag atcacgtaca cgaacatctt ctgagattcc agcagcagcc aaaattttct
    38881 tagatgttgc aagtccgata ccataaacat aagtcaatga gattactacg cgtttgtcat
    38941 ttggaatatc aactccagca atacgagcca tgttttctcc tttctatctt atccttgacg
    39001 ttgtttgtgt tttggatttg ctgggcaaat taccataaca cgaccattac gacgaataac
    39061 tttacagtat tcgcaaattg gtttgaccga tggtcttact ttcatttctt atccctccaa
    39121 gtttttcgat tatttaaagc ggtaagtgat acgtccacgt gtcaagtcat atggactcat
    39181 ttcgacagta acacgatctc ccgctaaaat acgaatatag tttttacgaa ttttaccaga
    39241 aactgttgct aaaatctgat gtccattttc aagttcaacc gtaaacattg cattcggcat
    39301 tgtatcaact actttgcctt caacttcaat cacatcgtct tttgccacgc aaaagcacct
    39361 ccataaattt cgattcgatg cctctagaca cagagacaac aattataagt cagactatct
    39421 cagtataaca tttgtagcgg atttttgcaa gtgcgaaaaa cgctttattt caaatttgtc
    39481 aatacttttt caatatctga gaagacatca ttgatatctt gattaccttc gatgtcatga
    39541 accaaacctt tggcacggta gtgagcaatg attggttctc cttgagcaat attaacatcc
    39601 aaacgacgtt ttactgtctc aggcttatca tcttcacgtt ggtagtaatc ttcttcttta
    39661 tagtcaactg gtgggttaaa gaccttgtgg aaagtttctc cagttacgcg gtggatgata
    39721 cggccactca aacgttccaa gaggctgtca gggttcactt caatattgat aataccttct
    39781 agttcaatgc caagttcagc caatgttttg tccaaggcat gagcttgttc aattgtacgt
    39841 gggtaaccat ccaataagaa tcctgtttct ttaatatcat cttgtgaaag gcgttctttt
    39901 acgattccat ttgtaacttc gtcaggaacc aattcaccct tgtcaatata tgacttagca
    39961 agaacaccca tttcagtttg atttgccatt gcagcgcgga acatatcacc tgttgagata
    40021 tgtgcaacat ggaattgttc tacgattttt gctgcttgag ttcccttacc tgcaccaggt
    40081 aagcccataa tcaaaagatt catgatttga tctccttatt ttatttttaa atagaagcaa
    40141 aaagtctttt cacccctatt taaaaacaaa atagaagagg ggagatccaa ctcttacaaa
    40201 tgtcagagtt taaacctcca ctccctcagt atttactgat tcagtaaata cttttattct
    40261 gttctgtcca tgaaaccaac atacttacgt ttcaataggt aaccttccaa ttgcttgatt
    40321 ccttcgatac ctgtagagat aatgatcaag agacttgttc caccaaaggc aacaacatca
    40381 gaaagaccaa atacatcttt agctgcaatc ggtaaaatgg aaatcacacc aaggaagagg
    40441 gaaccaacag ttgcaagacg acgaagaagt ttagacatat attcttctgt acctttacca
    40501 ggacgaactc catggatata ggcaccactc ttttgtaggc tctctgctgc tttttcagga
    40561 ttaatctgta caaacgtata gaagaatgta aagagaataa tcaacaaagc atacatggca
    40621 ataccagttg gagaagtagt tgccaacatc tcttgtgcta cccttaccca agcccaatca
    40681 tgacctgtgg cactcaaaaa ctgaagaata gccgcaggcg ctgcagtaat cgaactggca
    40741 aagataacag ggataactcc agcagggttt acctttaacg gaaggtaaga gctagatgga
    40801 gcaccttgtg caaccttagt atattggatt ggaattttgt attctgcttg ttgaacataa
    40861 gttgtaaagt aaataatcaa caatacagta ataatcaaaa tgattacgaa aatgatagat
    40921 gaagtgatac ggctacttgg gacgttcaca aagtagtcca catagatgcc ctgaatcatc
    40981 tctggaattg aggaaacaat cccggcaaag ataatcatgg aaacaccgtt tccgtatccc
    41041 ttatctgtaa tttgctcacc caaccaagtg acaatcatac taccagctgt taagatgata
    41101 ccaatcgtca gaaaaacttg tggagttaaa gcagttttaa tcaattgagc tccagccaag
    41161 gtattaaaac cagctgtaat cccgatagat tgcacaaaag cgagaactag agcaatataa
    41221 cgagtagctt gattcaattt tcttcgacct acttcccctt gtttacccca ctctacaaac
    41281 ttgggtaaaa tatccatttg caagagttgg acaacaatag aagcggtgat atagggacta
    41341 actcctaggg caaaaatcga aaagtttttt agggcattcc ccgacaccaa gctcaacatg
    41401 tttaagaagg ataatccact taaagcattc aagctattgg cattcacacc aggaactgta
    41461 atgctagttc cgatacgaaa gaccaaaacg ataaaaattg taaataaaat ttttgatcga
    41521 acctgcttga ctttaagagc ttctcttaat aatttaaaaa acataggtca cctctcttag
    41581 atgacttcta ctgaaccacc tttagcagtg atagcttctt cagctgattt agagaattta
    41641 gctgctttca cagtcaattt cttagtcaac tcaccgttac caagaatttt aatacctgac
    41701 ttttcagctt taacaattcc tgcttcgata agaacaactg gagttacttc agcaccatct
    41761 tcaaagacgt tcaattggtc aaggttcaca attgcgtatt ctttagcgtt gatgttagtg
    41821 aatccacgtt ttggaagacg acggaacaat ggagtttgtc caccttcaaa accaaggcga
    41881 actccgccac cgctacgagc tttttgacct ttttgaccac gaccagatgt tttaccgtta
    41941 cctgatgaag taccacgacc aacgcggtta cgtactttac gagaaccttc tgcaggtttc
    42001 aattcatgaa gtttcatttt tattttctcc tcttttgtaa aatgctagcg ccgataaggg
    42061 agaaaaggtt gtctccccca tcaactcgcc tatacgatgt catcatagat gactatatct
    42121 agttttaggg gatggtacag tgcacatccc ctaaaacttc attagtttac ttcttcaact
    42181 gttactaagt gagatactgc tgtaatcata ccacggatag cagcgttatc ttctttaata
    42241 acagagctgt tcaatttgcc aagtccaagt gctacaacag ttttacgttg tgatggaatg
    42301 cgtccgattg gagacttagt caaagtaatt ttaatttgag ccattttatc ccctttctta
    42361 tgccaaatca gaaactgaaa taccacgaag ggcagcaatt tcttcagcgc gtttcaattg
    42421 tttcaaacct tcaacagttg cacgaacaat gttgattgga gtgttagaac caagtgattt
    42481 agatgtaata tctgccacac ctgccaattc cacaacggca cgaactgcac caccagcggc
    42541 aactccagaa ccttctacag caggtttcaa caatacttta gctccaccga attctgaaag
    42601 aacttcgtgt gggattgttg ttccaaccat aggaacttcg atcaagtttt tcttagcatc
    42661 atctactgct ttacggattg cttctggaac ttcttgagct ttaccagtac caaatcctac
    42721 gcgaccattg tggtcaccaa caacaacaag agctgcgaaa cgaagacgac gtccaccttt
    42781 aacaactttt gtaacacggt tgacagcaac tacgcgttct tctaattcaa ctgcattgtc
    42841 tttaaatgcc attttctagt gtcctcctat tagaatttca atccgttttc acgagctgca
    42901 tcagccaaag ctttcacacg tccgtgatat agatatccac cgcggtcgaa caccacttct
    42961 gaaatacctt tagcgtttgc acgttctgca acgagtttac cgacagcaac ggcttgttca
    43021 gttttagttc cttttgaaac ttctttatca agagttgaag cacttgcgag cgttacaccc
    43081 gctacgtcat caatcacttg agcgtagatg cctgtattag aacggaatac gttcaaacgt
    43141 gggcgatcag cagttccaga gagttttccg cgaacgcgac ggtggcgttt ttggcggagt
    43201 ttgtttttat ctggttttga aatcacagtt ttcacctctt tagttttaaa tcgtgtgcta
    43261 tgcacaaagt tggaaaatag gttggtggtt gatgatcaac cactcaacat tatttacctg
    43321 ttttaccttc tttacggcga acgaattcac caacgtaacg gataccttta cctttatatg
    43381 gttctggtga acgaaggcta cgtacgtaag cagctgtttg accaactact tcttttgaaa
    43441 ttccgctaac aacgattgtt gttgggtttg gaagttcaaa agtaattcct tctggagctt
    43501 caacttcgtc tggatgagat ttaccaacag ccaaaacaag tttagatcct tgaagctgtg
    43561 cacggtaacc aaccccacgc atttcaagtt ctttcttgaa tccttctgat acaccaacaa
    43621 ccatgttgtt caaaagggca cgagtagttc cgtggatagt tttcatttct tttgaatcgt
    43681 ttggacggtg aagagttatt tcagtacctt ccacacggat ttcaatatct tttgagaact
    43741 cacgagtaag ttctccttta ggtcctttta cagttacaac gttgtcattg ttagcgagtt
    43801 caacaccagc aggcaacacg ataactttat taccaatacg tgacatgttt attttctcct
    43861 gttaaattgt caggccagaa cggccagttt tcacggggtt cagatactta tttagttcaa
    43921 gagctgaact gaacacgctc taagaactgt gcaaaataga taagttggct tgtttgtaaa
    43981 caaacttctc caacttccta attttgcttt gttctttacg agctttgtat cttgatttta
    44041 ccaaacgtaa gcgataacct caccaccaac attcttttgg cgtgcttctt tatcagtaag
    44101 caaaccttca gaagttgaaa ggatggcaat tccaagtccg ttaagaactt ttggaaggtc
    44161 ttcacgtttt ttgtagacac gaagtcctgg tttagaaaca cgtttcaagt tagtgataac
    44221 tttctcacca tttggtccgt atttaagaaa tacacggatg acgccttgtt tgtcatcttc
    44281 aatgatttca acgtttttta caaaaccttc gcgtttaagg atttcagcaa tccctttttt
    44341 gatgtttgat gcaggtactt caagtacttc gtgtttagct tggttagcat tacgaatacg
    44401 agttaggaag tctgcgattg ggtcagtcat aaccattttg tttttctcct cttactagta
    44461 gtttgcaagt tgcacttgct agttaataca tgatacaaag agcgtaacag cacgagcaaa
    44521 aatagacaat ttaatgcaga agcaatgctc caaagcaaat tggtcttttt tgccaaagct
    44581 gtagctcgtg ttcaaattgg caagcccaat tgaacccggg ctaaactctg tgtgaaaaag
    44641 ataaactttc ctagaaactt tagtttcttc gtcaagtttc ctatttttac ttggagtttt
    44701 gacgctcttg atatcttaaa ttaccaagat gcttttgtta caccaggaat ttgaccttta
    44761 tgcgcaagtt cgcgaaaagc gatacgactc agaccaaatt tgcggtaaac tgaatgtggg
    44821 cgccccgtaa ctctacaacg attatgtaaa cgagtcggtg aggcgttgcg aggtaattta
    44881 gataaacctt cgtagtcccc tgccgccttt aatgcagcac gtttttcagc ataacggtca
    44941 acaatttttt ggcgtttagc ctctctagct accattgatt ttttagccat tagatttacc
    45001 tcctatatta ttttgcaaaa ggcattccaa ggcctgtaag caatgcacgt gactcttcgt
    45061 cagtgttagc agttgttacg ataacgatgt caagaccacg agttttgtca acgtcatcga
    45121 agttgatttc tgggaagatt aattgttctt tcacaccaag tgtgtagttc ccgcgtccat
    45181 caaatgattt tgttggaaca ccgtggaagt cacgtacacg tggaagtgaa actgatacca
    45241 atttatccaa gaattcgtac atacgttcac cacgaagggt aacttttgca ccgatcgcaa
    45301 caccttcacg aagacggaag ccggcgattg attttttagc tttagtgata agtggttttt
    45361 gacctgagat aagtgccaat tcttcagcag ctttttcaag gcttttagcg tttgatacag
    45421 cttcaccaac acccatgttc aaaacaatct tatctacttt aggcacagcc atcactgatg
    45481 agtagttgaa ttgttctgtc aaagcaggaa ctacttcatt aagatatttt tcttttaaac
    45541 gatttgccat tatacttctc ctttccttcg tgattaatca agcacttcgc ctgatttttt
    45601 gttgtagcga acttttttac cgtctacaaa tttgtaacca acacgaccag ctacaccatt
    45661 tttgtccaaa acttgaacgt ttgatacgtg gatagctgct tctttctcga tgataccacc
    45721 ttgaggaagc tcgttagttg gacgttggtg tttcttaaca atgttaacac cttcaacgat
    45781 aactttgttt acttttggaa gggcagtaag gacaacagct tctgttccct tatctttacc
    45841 agcgattacg cgaactttgt cgcctttttt tacaaacatt aggtttctcc ttgatttttc
    45901 ttacgcccat aagggcaccc tagcttgaag ctaggggact agtttgttcc taaaaattaa
    45961 agtacttctg gagcaagtga cacgatcttc atgaagccac cttcacgcaa ttcacgtgca
    46021 actgggccaa agatacgtgt tccgcgagga gttttgtctt cacggatgat aactgctgcg
    46081 ttttcgtcaa atttgatgta tgaaccatca gcacgacgag cacctgattt agtacgaacg
    46141 ataactgctt taacaacgtc acctttttta accgcaccac caggagtagc ttgttttaca
    46201 gatgccacga taacatcacc gatgtttgca aatttacgtc ctgaaccacc aagaactttg
    46261 atagtcaaga tttcgcgagc accgctgttg tctgcgactt tcaaacgagt ttctgtttga
    46321 atcatttcag ttttctcctt tcaggtttga ttagatgatg accgcttctt caacaacttc
    46381 tacaagacgg aaacgttttg tagctgaaag cgggcgagtt tccatgatac gtacgatatc
    46441 gccttctttg gcaacattgt tttcatcatg agctttgtat tttttagagt agttaatacg
    46501 tttaccatag actgggtggt tacgttttgt ttcaactaca actgtgattg tcttgtccat
    46561 tttgtcagat acaacacgtc caacaagaac tttacgatta ttgcgttcca ttgaaatttc
    46621 tccttcccta gtctattatt tcgcttcaga ttgaactgtt ttgatacgag cgatttgttt
    46681 tttaacttct ttcaagcgag ctgtttgttc caattgacca gtagcagctt ggaaacgaag
    46741 ttcaaacaat tcttttttca attcgttttc gcgcttcgcg agttcttctt gagaaagacc
    46801 acgaagttct ttaacaaatt cttttacttc attaagtttc atgccttctc cttattctgc
    46861 ttcacgtttt acgaatttac atttaactgg caatttgtgg ctagcaagtc gaagcgcttc
    46921 acgtgcaatc tcttcagata caccagcgat ttcgaacatc actttaccac gtttaactgg
    46981 tgctacccaa ccttcaggtg cccctttacc agatcccata cgcacaccga tagctttagc
    47041 agtgtatgat ttgtgtggga agattttaat ccaaacttta ccaccacgtt tcatgtaacg
    47101 agtcatggcg atacgagcag cttcgatttg gcggttagtg atccagtggc tagttgtagc
    47161 ttgaagaccg tattcaccga atgctacttc ttttccacct tttgcttcac cgcgcatttt
    47221 tccacggaac tcacgacggt gtttaacacg tttaggtact aacattggtt atttacctcc
    47281 tttagtgttt ttacgagctg gaagaacttc accacggtag atccatactt taacaccaag
    47341 tttaccgtat gtagtatctg cttcttccca agcgtaatcg atatctgcac gaagtgtgtg
    47401 aagcggaaca gttccttcag agtatccttc agcacgggcg atatctgcac cgttcaaacg
    47461 acctgatact tgagttttga ttcctttagc tccagcacgc attgcacgtt ggattgcttg
    47521 tttttgtgca cgacggaaag caacacgttg ctccaattga cgagcaattc cttcacctac
    47581 aaggtgagca tccaaatcag gttgtttgat ttcgatgatg ttgatgtgta cttgttttcc
    47641 agtcaatttg ttaagttttg cacggagtgc atcaacgtta gcaccacctt taccgataac
    47701 catacctggt ttagcagtgt gaagtgaaac gttaactttg tttactgcgc gttcgatttc
    47761 aatagttgaa actgctgcgt cagcaagttc tttttgaacg aatttacgga ttgcaagatc
    47821 ttcatgaagg taatccgcgt attctttttc agcataccat ttggcatccc aatcacggat
    47881 gatgccgaca cgcataccaa ttggatgtac tttttgaccc acgattttac ctccttattt
    47941 ttctgcaaca gctacagtga tgtgagctgt acgtttgttg attggtgaag ctgaaccttt
    48001 cgcacgtgga cggaaacgtt tcatagttgg tccttcgttt gcgaatgctt cagatactac
    48061 caaattagct ttatccaaac caaagttgtt ttcagcgtta gctacagctg agttcaaaac
    48121 tttcaagatg atttcagcag ctttgtttgg agtgaatgtc aagattgcga ttgcatcggc
    48181 tacgctttta ccacggatgt tatcaagaac aagacgtgat ttacgaggtg aaacacgtac
    48241 tgtacgagcc attgctttag ctgaagtaat ttctgccatt tatgttctcc ttattttcta
    48301 cgtgttttct tgtcgtctgc agcgtgacct ttgtaagtac gagttggtgc aaattcacca
    48361 agtttgtggc ctaccatgtc ttcttggatg taaacaggta cgtgtttacg tccgtcataa
    48421 actgcaatag tgtaaccaat gaaacttggg aagatcgttg aacgacgtga ccaagtttta
    48481 ataacttttt tcttttcgtc gttagcttga gcttcaactt ttttcatcaa atgctcatcg
    48541 acgaaaggtc cttttttaag actgcgtccc atttttatat tttctccttt aaatgttgta
    48601 ccacagcggc ttgcactcct atggagcgct accgagctgg cggatttact agttgcttaa
    48661 gcgactagtt taatattatt tctcgttgcg acgacgaacg ataagtttgt cagatttcgc
    48721 tttcttgtta cgagttttaa gaccaagagc aggtttgccc catggagtag atggtgcttt
    48781 acgaccaact ggtgctttac cttcaccacc accgtgtggg tgatcgttag ggttcattac
    48841 agaaccacga actgttgggc ggataccttt ccaacggcta cgtcctgctt taccaaggtt
    48901 tacaagtcca tgttgttcgt ttccgacaac accaactgta gcacggcaag ttccaagaat
    48961 catacgaact tcacctgatt gaagacgaac aagaacatat ttaccttcag aacccaatac
    49021 ttgagcagat gcaccagcag cacgtaccaa ttcaccacca cgacctggtt tcaactcgat
    49081 gttgtgaatc aaagtaccaa ctgggatgtt agcaagtgga agagcgtttc cgactttgat
    49141 atctgcttct ggacctgaaa cgatacgttg acctacttca agaccttttg gagcgatgat
    49201 gtatgctttc acaccgtcag tgtagtgtac aagagcgatg tttgcagaac ggtttggatc
    49261 gtactcgatt gttttaacaa ctgcttcaac gttgtcttta ttacgtttga agtcaaccaa
    49321 acggtaaaaa cgtttgtgtc caccaccttg gtgacgaact gtgatacgac cgttgttgtt
    49381 acgaccagcc ttgctcttca atgcaacaag caatgatttt tcaggagtgc ttgttgtgat
    49441 ttcagcgaaa tccaaagaag tcatattacg gcgaccgttt gttgttggtt tataaacacg
    49501 aattcccacg atatttcctc cttagattat tcagcttcag cagcaaacaa ctcgattgct
    49561 ttagaatcag ctgtaagtgt gatgatagct tttttagttt tgttagtaaa accagtgtaa
    49621 cgtccaacac gtttagcttt tggttttacg ttgattgtgt taacattggc aactttaaca
    49681 ccttcgaaag cagcttcaac agcttgcttg atcaaaagtt tgtgtgcacg agtgtcaact
    49741 tcaaatacat attttcctgc ttcaagttga gccattgagc tttcagtgat gacaggtttt
    49801 ttgataacat catacaaatt cattatgcaa gaacctcctc gattttagag atagctgctt
    49861 gtgtgacaag aagtttgtcg ctatttgcga tgtcaagaac acttgcagtt gtagcagttg
    49921 caactttcac gtttggaagg ttacgagctg aaagagctgc gaattcattt ccttcttcaa
    49981 ggataacaag aactttagaa tcgatgctca atgctgcaag aacttttgca aattcagcag
    50041 tttttggagc tgtaaatgaa agagcgtcta cagctacgaa tttgttttca gcaacttttt
    50101 cagagtaaac tgatttaaga gctaggcgac gaactttttg tggaagtttg tagccgtatg
    50161 aacgtggagt tggtccgaag acaacaccac caccacgcca ttgtggtgag cggatagaac
    50221 cttgacgagc acgtccagtt cctttttgac gccatggttt gcgtccacca cctgatactg
    50281 cagagcggtt tttaacagcg tgtgttcctt gacgaaggct tgcgcgttgg ctgatgatta
    50341 catcaaacac aactgattca tttggttcga taccaaatac tgcatcgcta agaacaactt
    50401 ggccagcttc tttaccagtt tggtcaaata atgttacgtt tgccattgtg actgatttcc
    50461 cctttcttta ttatttacca gctttaactg ctgatttgat agtgataaga gatttcttag
    50521 cacctggtac gttacctttg ataaggataa cgttcttttc tggaacaact tgtacaactt
    50581 caaggttttg aattgttacg cggtcgccac ccatacgccc tgcaaggttt ttacctttga
    50641 atacgcggtt aggtgcaaca ggtcccatag aacctggacg acggtggtaa cgagaaccgt
    50701 gagccattgg tccacgtgat tgtccgtggc gtttgataac accttggaaa cctttacctt
    50761 tagaagtacc agttacgtca acaacgtctc cagctgcgaa tgtttcaact gtaatttcag
    50821 caccaacttc caagccttca acgtttttga attcacgaat gaagcgctta ggagccgtgt
    50881 tagctttcgc tacatgtcct ttagcaggtt tgttgctcaa tacttcgcgt ttgtcatcga
    50941 aaccaacttg gatagcgttg tatccgtctg tttcaacagt tttaacttga agaacaacgt
    51001 ttggagttgc ttcaataact gttacaggga tcaattcgcc agcttcagtg aagatttgag
    51061 tcattcccac ttttttccct aagattcctt ttgtcatgag aaaatagttc cttttctata
    51121 tttttattca aaaagttttt aacgagcgtt ttttatgctc aagtctaaga tacaaagggc
    51181 gtcaaactca acgtgaaaat aggaaactga cacagtgtct agcagacgcg aggaagttta
    51241 tctttttcac accgagttta gcccgtgttc aattagattg aaacaccagc ctctgctctt
    51301 ttatatttta gattaaagtt tgatttctac gtttacacca cttggaagat ccaatttcat
    51361 caaggcatca actgtttttt gagttgggtt aacgatatcg atcaaacgtt tgtgtgtacg
    51421 catttcaaat tgttcgcgag agtctttgta tttgtgagtc gcacgaatga ttgtgtagag
    51481 gctacgttca gttggaagtg ggattggacc cgcaacttgt gcacctgtac gagtagctga
    51541 ttctacgatt tttgcagccg ctgtgtcaag cgtacggtgt tcgtaagctt tcaaacggat
    51601 acggattttt ttgtttgcca tctttttctc cttttcgtct atttaagata ataggctagc
    51661 tccacaagaa aaccgacgcg gtgttgcgtg gcaatgcaac cgagcgtgtc gcaacctctt
    51721 gcatcaaaac taaggctgta atttacagca ccataataga ataacacaaa tcccctgcga
    51781 ttgcaaggga tttgttaggt tttttcgttt tttggggatg aaactgtttt cctacttttt
    51841 ctttctaaaa ttagggattt tcttctatat ttcttctata cataagttaa gtcctcttga
    51901 catcaaattt atcttctgat tggtccacta agatatccgc cttagactca gtttctttat
    51961 agtatcgcag atactgctcc cgtctcatct gatggctagc caatataaag gatgcatcgc
    52021 gatttctcac agtcgtatct ctagcaaggc gtcgctttaa ttcggtctcc tcatccgtgt
    52081 agaaacagat ggttttttca aagagttcct tgggtagaaa gccaacagac atcccttcga
    52141 caatcaaaat tggtttggct ccagacaaga cctcactagc cttccaaggt tcttcaattg
    52201 tcaagacatc catacccgcc tgcaaggcaa ggatatctct ctgcaaactc tccagttcat
    52261 gcgccactgg cagactggct gtcacctttt gattcggcgc gtccttgggt actaccaaat
    52321 agcgtccgga ggtgatatag ggatctgttt ctagcaaatt tactgtagta gaatctaaag
    52381 cttggtacaa ttcctgtgca aaggttgatt tacctgaagc tccatgaccg tatattccca
    52441 gtgtcctgac tttccccgtc tcgatctctg agactagttg gtctactaag tcttttttct
    52501 tcattctctc ttcacctgtt catagatttc ttttccgtca ttgagcttgt cccaaatcac
    52561 tacttgccca cttttgagcg atttttgcac atcgataatt cgttggttag acgaacctcg
    52621 aaactggagc ataagatttc tcttagttcg atcatatctt ccatcgacaa gaatgtcaat
    52681 cagtgacaag agttccagtt tatctggagt ttccaacatc atttcttccc aagtgtagcc
    52741 cgtccaggac cagatgtcct tgtctggcaa ttccttccga atccgcttaa caagtggcaa
    52801 gagaatccca gtattaagaa aaggctcccc tcctagtaag gttaacccct gaacataagg
    52861 ctgggcaaga tctgccataa tctgttcttc caactcagct gtatagggaa tgcctgcatt
    52921 gaaggaccag gtcgctacat tataacaccc ctcgcagtga aacatacagc ctgctacata
    52981 gagagagtta cgcacgcctt cgccatcaac aaagttaaag gccttgtagt caatgatacg
    53041 accttgacta agttcctcgc ttttccattc ttgtggtttt ggattattca ttcgctacct
    53101 ctatccaata acgctcgact ccattgcgag catcctcaaa tattccacca tttgctagaa
    53161 tgactgctct gctagcagga ttattcacgc tacaggtcac cagagctttc ttgatgttct
    53221 tttccttagc aacttgcaag ccctgacgga gagtctcttt tgcataaccc ttgcctcttt
    53281 cagatggacg gatggagtag ccaatgtggc cagcataatt cagtaacccc tcattcagtc
    53341 tcaatcgcag attcaaaaat cctagagcac aacctgattc accaaacaat acgaactgta
    53401 tcgatggaac gcgattttca ggtagccata ttcccatttc tttattacgg ttaccttcta
    53461 gccactcctc gtaagaaaaa tcctcaacat cccaaaatcc tccatcatgg gctgattggc
    53521 tcttttcaaa ctctgtcatc atatctaaaa cagctttctt atccgctaat cttggtctgc
    53581 gtaattccat ccttacctcc cactatgaga aaagcgagag ctggctctca cttttatttt
    53641 ttcttgttct tgtttaaaaa ttgttctctg aagctaagca agcgttcttt cttaccagct
    53701 ccacctttag agtgtttctc gtgataacgg gtcacttgtg cgcgcccctt atcgtctagt
    53761 tgatattttc ccatttcatt tctttctaat ttgttacttg atgcccagct attttaatcg
    53821 ttgaaccatt catatgtttg acacgcgcag cgatttcctt gtgacgcccg ttgaccatcg
    53881 gtcttgcttg aggattacct aggtagccac aagttcgctt caccacatct actgttttag
    53941 ggtcgctatt gccacagttt ggacaagcaa accctctctc agttggttca aaatcccctt
    54001 caaagtcaca cttgtagcaa cggtcaatcg gagtattggt gcctagatag cctacacggt
    54061 cataagcata atcccagaca gcttccaagg cctttggatt ttgctgaagg actggatact
    54121 cacaataatg gatgaaacca cctgacgcac ctgcttctgg atagactttc tcaaagtcca
    54181 atttttcaaa cggtgttgga tttttacgaa catcgtagtg gaaagagttg gtgtagtatt
    54241 ccttgtctgt gatatcagga atagagccaa acttgtctat atctagtcgg cagaaacggt
    54301 ctgtcagact ttcggatggt gttgagtaga tagagaaatg gtagccatat tggtctgacc
    54361 actcttctac acggcgtttc atatcgtgaa tgatgtctag cgtgaattcc ttagcatctg
    54421 gattactttc ccagctgtta ccaaagaaaa ctgtcgctac ttcatacaag ccgatatagc
    54481 ccagcgaaac ggtcgcacga caattcttaa agagctggtc aacactttct tctttaccta
    54541 gacgatggcc aaaagcaccg tactgataaa ggataggagc attcgctggt gtcgcctctt
    54601 tagtgcgttc gacacggtaa acaagagcat cttctgcgat attcattcgc tcgttgaaga
    54661 tttcccagaa cttattcata tcaccttcag actcaagagc aatacgaggc agattaaccg
    54721 tcacaacacc cagattcatg cgacctgaat tgacttctac accattttca tccttccatc
    54781 cttggaggaa agaacggcaa cccataggca ccttgaagga tcctgtcaag tcaacaatct
    54841 tatcgtaaga taaaacatct ggatacatcc gtttggttgc acactcaaga gccaactgct
    54901 tgatgtcgta gttgggagtt ccttcctcta aattaaggcc tcttttcaga gtgaaaataa
    54961 gtttaggaaa gatagccgta cggtgttctg aaccaagacc cttgatgcga atgtttaaaa
    55021 tagctttttg aatttctcgt tcaaaacgac tggttcccag accaaaacct agcgaagtaa
    55081 aaggtgtttg tccatttgaa gtgaagagag tattgatttc atactcaaga gattgcatgg
    55141 catcgtagat gtccttttgc gttttcttcc aagcgtaatc ttcccgtttt tcaggcaata
    55201 cccactcttc tgcatctttg agatgttttt gatagttctt ctctgcataa ggcgccaaaa
    55261 tttcatcgat acggtcagct gaacagccac cgtactggct agaagcaacg ttggcaatga
    55321 tttgagaaat ctgtgctgtc gcagtctgga tagacttggg actctctacc tctgcatttc
    55381 caatcttaaa accattttcc aacataccct taaaatcaat caaacagcag ttggtcatag
    55441 gggtataggg actgtagtcc aaatcgtgat agtggatatc ccccttttgg tgggcattgg
    55501 ctacgtgctt aggaagcatt tgcagtccga ttgatttccc aacaatccct gctgtcaaat
    55561 cacgctgagt gttaaagaca tcactgtctt tattagcatt ttcattgaca actgtctggt
    55621 ctttgttgag aagtttatga atactaaagt tgatatccgt cgcttttgag cgctcaaaat
    55681 ccctctgtgt ccgataagta atatactcct cagccagcgc atattctttg gcttcaagga
    55741 gttcatgttc tacgatattt tgaatttcgt aaatcttaat tccctgtgga aagcgactat
    55801 gaatttctgt aataattctc tcagtcagat cattgaggcg tttttcaacc aggggtgtca
    55861 catccataac cttgtcagct gccttgtgga gagccttgtc aatcttgtcc acatcaaata
    55921 caacacgtct cccatctcgt ttctcgacga agagagttgg caaaattgta attttttctt
    55981 ctagtgcaat catatgcatg ctcttttcta aaatgttctt tctaaaaagt attataccac
    56041 tctaaaaaga aaaatcaata tcttgtgtta aaaatcctaa aatttttaaa aatacacaag
    56101 atattgattt ggttatttaa ttttatagac tttaaactct gagtaatcag tctttacttg
    56161 ttggtaattt tcttttaaaa gtgtttccac ttcagaccag actaccacct tgtcatttac
    56221 cacaatcacc ttaggttgtt tttctttcaa gtcattaagt aacttattac gattttcctc
    56281 agttgctgta tagtgcaacg gggacaaaat cgccgatggc gacaagcgct cactcttacg
    56341 ataaagagtc gcagtatcat cccatgcata gatagcatct tccgtatttg tctcctcttt
    56401 taccaaatca gcaagacgat tacgttcttg ataaggtact ggataaagga caaacttcat
    56461 caaaaaagga acagacaaaa gatagacaaa cccaaaaact agcagatata gatttccttt
    56521 gaagaaacga ctccataggc taactggcgt ttctcttcgg cttcttctag cactacgccc
    56581 cctctgttga ctcttgatat tggttaccaa taaaagtagg aaaacaggga agacgaccat
    56641 caaataagaa gcgtgcagag gctcttgtga aaagaaaagt aaaatcaaac ctaaaattac
    56701 cacaagactt gcaggaaccg aaatgacgta tagtttaaag ggtttggatt gaaagagacc
    56761 taaaaagata caaaccacaa atcccaaacc aagggacaac aagccataaa aaaacatatt
    56821 ctctaaaatt ttagaagtaa aatcaaagcg aatactgtca ataggataac gaataccact
    56881 aatcgcatcc ccaaaacttc ctgttgcagc actatagtag gcagttggat aaaagacaag
    56941 tgaaaaacct aaagccactg caagaaactg ataaaaccca tgcgcaaagc gtctatgccc
    57001 aaggttaaag accaacaagc ctaaactcac tacagcaata aacaggagtg atgataaggg
    57061 agcaaagaaa aatccgcctg ccaaagctaa cccaatccgt acaaatccct tatcatggct
    57121 tggattgctt aggtaagccg caactaaact aaaggctgcg aataagaaag gaagcgctaa
    57181 aagagtcgca tagcctccac caaaagcaag acctgtaact agcatgtaaa aaatagtcac
    57241 cagatgtcca gcttggtctc cttgctctgt caaggtgtcc gctgatctaa aaaggaaaaa
    57301 tcctcctgct accaaggcta accactcaaa gatggcaaag aaaaatccgc cctgactcac
    57361 gtaagtcagc aaataataaa gcaaaccttg acttccataa tagtcgctgt aaatcttccc
    57421 tgtctgatga agcgcccaac ctgcataaaa atcctgcact tcttgtgcac tcattaagtc
    57481 gagtaatagc ggtactccta gagttatccc cgttacaagc gtactccata gtaaaatttt
    57541 caccaaagga agacgacttg attcacgatg atgcgattct tgctcgattt ggtattctag
    57601 aggttcacga ttctccttat gaacttcttc tactctacca tacacactca tatcgtttct
    57661 cctgttcaat ttatctgtct tagtatatca aaaagtccta ggattgtcag cttgcgatgg
    57721 ctgtttgagt gattttttgc atagtttcac aaaggtttca atttcccgaa agcggtgtaa
    57781 atgtgtctgt ttgaactcaa tcatatcatt cacttcttcc ttagttagca tgaattcccc
    57841 tcctctttct ttctattcgt aaaagacaag aaaaataggt cgggtttgta tcctaagttc
    57901 tagatttctt ataagatagg ggcgaatagc tgggcgattt cttttacaag cttttgatac
    57961 cagctcgttt tgatagaatg aggatagact tcctgagaat ctgcaaatat atttcgaaaa
    58021 tctcgggcga tttcccttat agaaggagtt ttatagagta agactgcatt ttcataatgg
    58081 tgtaccaagc tccggtagtc gagattgatt gtccccacca ccgcaaaatc ttcgtctacc
    58141 aacatctgct tactatgaat aaaacctgga ctatactcat aaatccgaac accagcagaa
    58201 agaaggtcgg gataagctcc tctcgtgact aactgaatga acttcttatc tggtatgtaa
    58261 ggggtgataa ttcgaacatc gacccctctc atagctgcat ttttgattgt ctctgttaaa
    58321 tcataatcta taatcaaata aggtgtcgta atatagaccg attccgttgc ttgattgatt
    58381 aaactctgat aaactttttt ccctacctgc gctcgaaaaa ttggcttggg tccacttccg
    58441 tatggaatgg ttaaaccgtc actcgggata gaatgatttt ctaaatgata ttgatcaaaa
    58501 tcactaattt ctcctcgatt aatgtaccaa gtggtcaaaa ataagcgtgt cagggctttt
    58561 actgctagtc cgtctaagcg aattccacta tccttccaat aaccaaatct ctcgacgtgg
    58621 ttaatgtact catctgccag attgacccca ccagtatagg ctatctgacc atcaacaatc
    58681 aatatttttc tatgatctct gttattataa gccactgtca aacgaggaat aactttattg
    58741 aatttatggg cctcgatgcc cagctgacga agtcgatgtg cataatctcc tgttaaagta
    58801 gccatacagc cgatatcatc atagagcatc ttaacctcta caccctgagc taccttttgc
    58861 tctagtatat ctagtatgcg attccacatc aaaccttctt ctataatgta atattccaag
    58921 aaaataaatt tctcagcctt tttaagatct tctaccatct ttttccacat agcttctccg
    58981 ttaggaaaaa atgtagaggc agtttgatca tagatgtcag cattggtatc catactcaat
    59041 aaggacttga tgactccata agctgccttg tcactttctt ttaatttctc ttttagtagc
    59101 tggctatttg cttcttggaa atgcatagag cctagcttct tcagttgttt gatttctttt
    59161 ttggacaatc gcctttcacc aaacatcaga tagagtaagg gaccaaatac tggcacaaag
    59221 gctactaaca accaggttac cttattctca ggagtcgtat tacggttgac tatcgaaatg
    59281 attgtgatga tactcagtag aatgaggaca gtaatccata aaatgggagc catgcgtcct
    59341 aaataaagaa agagaccaaa aacaagacaa agttcaagta gcataatcga aagactaaag
    59401 ccatacttgg acatcaataa ttgaaatttc ctatatttca tatcccctcc ttgatagttt
    59461 gaatgctaaa aacaagtaat tgatactagt ataacgcagg tattcggtag aaggcaagta
    59521 tttatgatta ttttctaggt aaaagtaaaa aggctgaaaa atccagccct ttctacttgc
    59581 atagtcttat ttagttaaaa cgatttggtc cgatgggtct ctatccatat catctacgac
    59641 gatttgatta ccgtttacag tgtaaatttt gacatcatcg ccaataatca tgcgttgatt
    59701 tgctgaatca aatgtaacct gtttgacttt ctgatccccg tcagattcga gctcagtcca
    59761 tgtaccagtc gttccagtta ccactaaagt gatacggtct ccgtcatcct gaccagtata
    59821 agtcccatca atatcggtcg gttgagcaac aggttggttt tgtactggag ccgcttgatt
    59881 ttggttacct cctgcatttg cagtattggt attctgttga gcaggttgtt gagcaggctg
    59941 ttggacctgc tgctgagcaa caggctgttg aacctgttga gctctttgtg aacaagctac
    60001 taagagacta aaacttgcta gagaagcaag agaaagtgtg aatcttttta atttcatgat
    60061 gaatttcctt tctgctacca atttagagaa attttctcta accagcaatt cccctagtat
    60121 aacaagttca aacaaggggg tcaatttatc tgctcacggt ccagcaggta gccccgtact
    60181 tctgagataa aatagagaga ccctgtaacg aacagcaagt cttgagcgtc tgccctttct
    60241 tcgaaactgc tgataaattc tcggcaagaa gaaactatat cgtaacctgt cacatccctt
    60301 tcgtccaaag ccccctgata gtcaaagccg gtcaccttga gttccacctg aggcaatttt
    60361 tcagtcaggt aacctaacat cccttgataa tccttccgtt tcaaggctcc aaaaaggatt
    60421 tgaggtcgat agccttcctg ctctttttct ttgataaact caaccaagcg agtcaaggca
    60481 gggaggttat gagcaccatc caagtaaatc tgtgggcgaa tacgctccaa gcgaccagcc
    60541 caatgggtct gttccaaggc ctttcttaca gcctgttcat caacagcttc ctttccttct
    60601 cccataaaaa gaagaaaagt ttgcaacgcc aaggctgcat tctcctgctg atagactcct
    60661 tctaagccta tgttcagctg tgaaaaattt agtaaagagc ttgaaaaatc accattcagc
    60721 attaaaaaat cttgacctgc ctgataaagg ttaacagcta aagattcggc ttttttctga
    60781 cagacaagcc tagcttctgg aggcaatttc gcaatcactg ccttcttacc agccttgaaa
    60841 ataccagctt tctgctctgc aattgcttct agactatcac ccaaggtttc ttgatggtca
    60901 agcccaatgg aggtgatgac agcaagctct ccagttacca cattggtcgt gtcaagtaag
    60961 ccaccaattc ccacttctaa taaaaccaaa tccacctctt gctccctaaa ataaagaaaa
    61021 gcaaccaagg tcagcatttc aaaaaaggac aactggtcag gagtttgcag aagcgttttc
    61081 tccatctcct tgacctgatc agccaaacgg ataaagtctg cgtctgctat aggttgccca
    61141 ttaatgcaga ttcggtcatt gatagagacg atatgagggg aggtaaaggt cgcaactttt
    61201 ttgccatgcc ccataaataa ttccctcata aaagcaatgg tagatccttt cccattagtc
    61261 cctgttacgt ggataatagg gtaagactgc tcaggatttc ctaacaaatc caccgcttgc
    61321 tgtattcggc ccaaacctga tcgaaaattc aaaccaatcc gactatgaag ccattcttct
    61381 acttcaaaca tacacatctc cttgacaaaa gtccaatcaa ttatcgcatt aaagtatggt
    61441 tactaataaa aacaaggcca ggattttcgt cccgacctct tacctggtta gctaataact
    61501 agccactatg aatgtgaata tgggctaaaa acatccacta gatgttccaa ctcttcccca
    61561 tttctgggag ttgggctgat acagtctccc agactgtatc actcctccat aaagctgttg
    61621 aagacttctt caatcatgtt ccattcgtct tctgagtctt ctgggattgg ttgcaattcg
    61681 ccttctgttc catcttcatt ttcaatgaat gagtaagctt ggatttcaac ttgtccgtct
    61741 tcgtcttctt ctgcgttaac tggtactaga agaacatagt ttttaccaaa ttcttctttt
    61801 ccatcaattg tcaaaaggat ttcaaacaag gtttcatttc cttgctcatc tactagtgtg
    61861 attagttcac gttcttcgtg gtcgtggtta tgatcgtgtg acatagcctc tcctttatat
    61921 taaaattttc tatctaaata attttgtaaa atcagctgag ctgctaactt atcaatgact
    61981 ttcttgcgct tattgcgact gatatctgct tgttcaatca acatgcgctc agcagccact
    62041 gttgtcaagc gttcatcctg atagtctaat ggtaaaccaa aaaactcttc tagctttgct
    62101 ccgtaggctt gactagcttc tacgcgcggt ccacttgtat tgttcatgtt tttaggcaag
    62161 cccactacaa atcgttccac cttgtaagta tcaaccaatt ccttaacgcg gtcaaaacca
    62221 aattggcctt gttcttcatt tatctggatg atttcaagcc cttgagctgt aaaaccaagc
    62281 ggatcgctaa tcgccacccc taccgttttt gaaccgacgt ccaatcccat aattctcata
    62341 ggttatagat cgactccttg tcctttgagg tagtagcgaa ccaattcctc aacgatttca
    62401 tcacgctcat acttacggat ttgatttcgt gcattattat aacgaggaac gtaggcaggg
    62461 tctccactca atacgtaacc tacgatttgg ttaattgggt tgtaaccctt atcgttcaac
    62521 gaagcataaa catctgtcaa agtttcgcta atttcttttt tattggaatc gtccaattta
    62581 aaacgtactg tttcttcagt aaatcccatt ctaacaccct ctttccttag aatagtacca
    62641 ttatagcata attccttacc ttctacaatt caggcagtct atttatttgg attttctatt
    62701 gttctgtcgc gccatttgcc aatctatctg aaatatattt gcttggttca tttttcaaaa
    62761 gattttccaa accaatattc ttcagatgtt ccaactggga agccttcttg acatccagaa
    62821 cttgaaaatc aaaactagtc gttgtttgaa gttccgttgc gctcaatagt tttgtttcaa
    62881 gtttgaaacc tgccaattta cgagcttcaa tgatagactt atccttctcc tccgcttcaa
    62941 gaagagcttt ttgagtttcc tccactccat gttggtcaat ataagtcttc aactcatcag
    63001 agactggacg tttttgttgg atagttagac tcaaaaaagt cttgtccttg tatgtaatgg
    63061 tttgtgtctg ctggctacca tcatctgact tgggcaagac caaagtctta gttacaactg
    63121 tattcttctc agcattttca ataactggca atgccgactg aagcgtatct ttttctgttt
    63181 ttgtagctgg tccagtttct tttttctgtc cgcaaccaac caggacaaaa aggaaagcta
    63241 gactaacaag aactattttt ttcatttctt tcttctttct ttttgaaatt aaaatagaat
    63301 aagactggga agtgctccca gccttgatgt ttatagagct gcacgcaaac gtgcttctgc
    63361 attttctaca ttacggacag agcgtggtag gaaggcacga atatcgtctt ccttgtagcc
    63421 aacttgcagg cgtttttcat ctacaaggat tgggctcttt aaaattctcg gtgtttccat
    63481 aatcagattg agaacttcat tgacactcaa atcttcaata tccactccaa gggctttggc
    63541 atagcgattt ttagacgaaa cgatgctggc tattccgtta tctgttttgg ttagaatatc
    63601 cagtaattct tctctcgtaa ttccttcttt accaaggttt tgttctttat aacttaactg
    63661 gtgggcattg agccaggttt ttgctttttt acagctagta caacttgaga ctgtataaat
    63721 tttaatcatg tacctacccc tttcgctaca tgttactatc agtttagtct attataccat
    63781 aaaaaacatc cgacttgcga cctattttta at
//
LOCUS       DAVBCN010000004        54845 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVBCN010000004 DAVBCN010000000
VERSION     DAVBCN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 54845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54845
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.004"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(77..1966)
                     /locus_tag="VQ497_000218"
     CDS             complement(77..1966)
                     /locus_tag="VQ497_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HET2516587.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWSRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGLEKTLQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2104..3669
                     /locus_tag="VQ497_000219"
     CDS             2104..3669
                     /locus_tag="VQ497_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET2516588.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIALPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            3669..4409
                     /locus_tag="VQ497_000220"
     CDS             3669..4409
                     /locus_tag="VQ497_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2516589.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(4518..4733)
                     /locus_tag="VQ497_000221"
     CDS             complement(4518..4733)
                     /locus_tag="VQ497_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516590.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(5604..6854)
                     /gene="ilvA"
                     /locus_tag="VQ497_000222"
     CDS             complement(5604..6854)
                     /gene="ilvA"
                     /locus_tag="VQ497_000222"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HET2516591.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(6904..7260)
                     /locus_tag="VQ497_000223"
     CDS             complement(6904..7260)
                     /locus_tag="VQ497_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET2516592.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEIFGDE"
     gene            complement(7498..7734)
                     /locus_tag="VQ497_000224"
     CDS             complement(7498..7734)
                     /locus_tag="VQ497_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516593.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKY"
     gene            complement(7841..8863)
                     /gene="ilvC"
                     /locus_tag="VQ497_000225"
     CDS             complement(7841..8863)
                     /gene="ilvC"
                     /locus_tag="VQ497_000225"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET2516594.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(8929..9405)
                     /gene="ilvN"
                     /locus_tag="VQ497_000226"
     CDS             complement(8929..9405)
                     /gene="ilvN"
                     /locus_tag="VQ497_000226"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET2516595.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(9398..11098)
                     /locus_tag="VQ497_000227"
     CDS             complement(9398..11098)
                     /locus_tag="VQ497_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HET2516596.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDILPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(11298..12965)
                     /locus_tag="VQ497_000228"
     CDS             complement(11298..12965)
                     /locus_tag="VQ497_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HET2516597.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(12968..13333)
                     /locus_tag="VQ497_000229"
     CDS             complement(12968..13333)
                     /locus_tag="VQ497_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2516598.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(13490..13678)
                     /locus_tag="VQ497_000230"
     CDS             complement(13490..13678)
                     /locus_tag="VQ497_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET2516599.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(13794..13970)
                     /locus_tag="VQ497_000231"
     CDS             complement(13794..13970)
                     /locus_tag="VQ497_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HET2516600.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     DGMWRAENVTQDLI"
     gene            complement(14081..14230)
                     /locus_tag="VQ497_000232"
     CDS             complement(14081..14230)
                     /locus_tag="VQ497_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HET2516601.1"
                     /translation="MDKVETTIKSLCVHHYNLKFYVFNDDLPREWFQLMEKRLETLNS
                     EIVNV"
     gene            complement(14542..16086)
                     /locus_tag="VQ497_000233"
     CDS             complement(14542..16086)
                     /locus_tag="VQ497_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HET2516602.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            16299..16601
                     /gene="gatC"
                     /locus_tag="VQ497_000234"
     CDS             16299..16601
                     /gene="gatC"
                     /locus_tag="VQ497_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET2516603.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEQDNYYIKVPAILDDGGDA"
     gene            16601..18067
                     /gene="gatA"
                     /locus_tag="VQ497_000235"
     CDS             16601..18067
                     /gene="gatA"
                     /locus_tag="VQ497_000235"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET2516604.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDANNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDTKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            18067..19509
                     /gene="gatB"
                     /locus_tag="VQ497_000236"
     CDS             18067..19509
                     /gene="gatB"
                     /locus_tag="VQ497_000236"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET2516605.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDLHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            19638..20198
                     /gene="efp"
                     /locus_tag="VQ497_000237"
     CDS             19638..20198
                     /gene="efp"
                     /locus_tag="VQ497_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET2516606.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            20220..20609
                     /locus_tag="VQ497_000238"
     CDS             20220..20609
                     /locus_tag="VQ497_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2516607.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGVYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            20602..21024
                     /gene="nusB"
                     /locus_tag="VQ497_000239"
     CDS             20602..21024
                     /gene="nusB"
                     /locus_tag="VQ497_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET2516608.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTNVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKVGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(21073..21450)
                     /locus_tag="VQ497_000240"
     CDS             complement(21073..21450)
                     /locus_tag="VQ497_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2516609.1"
                     /translation="MVLSNGQPTFLSTILSFVLLVIIGPVFEETLDRGYFMNTFFPKS
                     KYYLDVILSGLIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDNLRLTILCHSFF
                     NFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(21791..21976)
                     /gene="briC"
                     /locus_tag="VQ497_000241"
     CDS             complement(21791..21976)
                     /gene="briC"
                     /locus_tag="VQ497_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HET2516610.1"
                     /translation="MTGTETFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
     gene            complement(22135..22902)
                     /locus_tag="VQ497_000242"
     CDS             complement(22135..22902)
                     /locus_tag="VQ497_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HET2516611.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(22899..23765)
                     /locus_tag="VQ497_000243"
     CDS             complement(22899..23765)
                     /locus_tag="VQ497_000243"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HET2516612.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(23802..25169)
                     /gene="accC"
                     /locus_tag="VQ497_000244"
     CDS             complement(23802..25169)
                     /gene="accC"
                     /locus_tag="VQ497_000244"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET2516613.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(25181..25603)
                     /gene="fabZ"
                     /locus_tag="VQ497_000245"
     CDS             complement(25181..25603)
                     /gene="fabZ"
                     /locus_tag="VQ497_000245"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET2516614.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGTLTFAIGN"
     gene            complement(25600..26085)
                     /locus_tag="VQ497_000246"
     CDS             complement(25600..26085)
                     /locus_tag="VQ497_000246"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET2516615.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVASEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(26088..27323)
                     /gene="fabF"
                     /locus_tag="VQ497_000247"
     CDS             complement(26088..27323)
                     /gene="fabF"
                     /locus_tag="VQ497_000247"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET2516616.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(27345..28076)
                     /gene="fabG"
                     /locus_tag="VQ497_000248"
     CDS             complement(27345..28076)
                     /gene="fabG"
                     /locus_tag="VQ497_000248"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET2516617.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            complement(28110..29030)
                     /gene="fabD"
                     /locus_tag="VQ497_000249"
     CDS             complement(28110..29030)
                     /gene="fabD"
                     /locus_tag="VQ497_000249"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET2516618.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(29023..29997)
                     /gene="fabK"
                     /locus_tag="VQ497_000250"
     CDS             complement(29023..29997)
                     /gene="fabK"
                     /locus_tag="VQ497_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HET2516619.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            complement(30116..30340)
                     /locus_tag="VQ497_000251"
     CDS             complement(30116..30340)
                     /locus_tag="VQ497_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET2516620.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(30400..31374)
                     /locus_tag="VQ497_000252"
     CDS             complement(30400..31374)
                     /locus_tag="VQ497_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HET2516621.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANNRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(31374..31808)
                     /locus_tag="VQ497_000253"
     CDS             complement(31374..31808)
                     /locus_tag="VQ497_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2516622.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(31902..32687)
                     /locus_tag="VQ497_000254"
     CDS             complement(31902..32687)
                     /locus_tag="VQ497_000254"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET2516623.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            33012..34376
                     /locus_tag="VQ497_000255"
     CDS             33012..34376
                     /locus_tag="VQ497_000255"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET2516624.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRNYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            34379..34750
                     /locus_tag="VQ497_000256"
     CDS             34379..34750
                     /locus_tag="VQ497_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HET2516625.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            34964..36238
                     /gene="serS"
                     /locus_tag="VQ497_000257"
     CDS             34964..36238
                     /gene="serS"
                     /locus_tag="VQ497_000257"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET2516626.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGTDEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(36263..37162)
                     /locus_tag="VQ497_000258"
     CDS             complement(36263..37162)
                     /locus_tag="VQ497_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HET2516627.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACSGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(37272..37820)
                     /locus_tag="VQ497_000259"
     CDS             complement(37272..37820)
                     /locus_tag="VQ497_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET2516628.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(38038..39360)
                     /locus_tag="VQ497_000260"
     CDS             complement(38038..39360)
                     /locus_tag="VQ497_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HET2516629.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGARFIWLYRVFFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(39451..39539)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(39703..42039)
                     /locus_tag="VQ497_000261"
     CDS             complement(39703..42039)
                     /locus_tag="VQ497_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HET2516630.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVHFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(42143..42691)
                     /locus_tag="VQ497_000262"
     CDS             complement(42143..42691)
                     /locus_tag="VQ497_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET2516631.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIFYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(42688..42990)
                     /gene="zapA"
                     /locus_tag="VQ497_000263"
     CDS             complement(42688..42990)
                     /gene="zapA"
                     /locus_tag="VQ497_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET2516632.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            43077..43958
                     /locus_tag="VQ497_000264"
     CDS             43077..43958
                     /locus_tag="VQ497_000264"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HET2516633.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQLVEENRGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TSDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLSVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            43962..44576
                     /gene="lepB"
                     /locus_tag="VQ497_000265"
     CDS             43962..44576
                     /gene="lepB"
                     /locus_tag="VQ497_000265"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET2516634.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRLWPITRIGTF"
     gene            44704..47070
                     /locus_tag="VQ497_000266"
     CDS             44704..47070
                     /locus_tag="VQ497_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HET2516635.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLKTIQKHITTIEKELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSSISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(47118..48401)
                     /locus_tag="VQ497_000267"
     CDS             complement(47118..48401)
                     /locus_tag="VQ497_000267"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET2516636.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     ATAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(49155..50291)
                     /locus_tag="VQ497_000268"
     CDS             complement(49155..50291)
                     /locus_tag="VQ497_000268"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HET2516637.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQVLDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIDQIVEYI"
     gene            complement(50353..50790)
                     /locus_tag="VQ497_000269"
     CDS             complement(50353..50790)
                     /locus_tag="VQ497_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2516638.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKKDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            complement(50792..52747)
                     /locus_tag="VQ497_000270"
     CDS             complement(50792..52747)
                     /locus_tag="VQ497_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET2516639.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQTYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTIKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            complement(52771..54540)
                     /locus_tag="VQ497_000271"
     CDS             complement(52771..54540)
                     /locus_tag="VQ497_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HET2516640.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGAFPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
     gene            54754..>54845
                     /locus_tag="VQ497_000272"
     CDS             54754..>54845
                     /locus_tag="VQ497_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2516641.1"
                     /translation="MKLTYDDKVQIYELRKQGYSLEKFSNKFGI"
BASE COUNT        16239 a        12405 c        10207 g        15994 t
ORIGIN      
        1 ctattgactt ttcttatagt ttgtctttct tctatcttcc attatatcat actttttcag
       61 tctatggtag ctctctttaa aaggctccga tactgccgcc acctccgcct ccagagaagc
      121 caccgccaga acttccactt ccagaagata cggagtaggt acttgctgta tttgcgacac
      181 tagcataatg gctcatttgc gcgcttgaat gataaaacat actgtgccag ccataagcta
      241 catagagatt gatatctgga ttttcaactt gaatctgatg aaccttcatc aaatgactaa
      301 ccttgtccgc atagccaaat aaggttgcat agaccaagag acgattccaa acaacaatac
      361 tttccaattc agccttatcc agtcgtgcaa tctcacgtaa catattttca aaactggtcc
      421 agagatagta gacctcagct cccgcttcat ttagaacacc atcacgatta tctagtcgaa
      481 gcttccaata atagaaaaca gacaaaacta accctagaaa accaagtatt ggcaaaggga
      541 ggtaaagata gccatgaacg tctaaactgt acaagaacaa accaaatccg ataaataggg
      601 gcaagatagt caagacaccc atacccactt gcaaagtctt ttccaaacca gttaaaggac
      661 gatagtaatc tgggagcccc cagaaggaaa ctcgttttct cactccttct tgcatctggt
      721 tcaatacctc ttcaaaagaa gatttgagtt gaagccctct tgcttgaatc cgtttttcat
      781 cagaaacttt ggctctacga taaagactat cagatacctt gtaatccgca aacaaattgg
      841 aaagagtggc ttcttttttg cctgaaaagg ctagatttag acagtctctc tcaaatcttg
      901 acaaaccatc ttctttcacc aatctcaagc caactgcatc tccttctgaa ataatagaga
      961 cattcccacg gtctatcaca tctagcaagg tagcttgaat aagttgatca aaggtgaatt
     1021 ttccagctcc cttgaccaag ggactcactt cctccaagga ggtcgagtag actgcttctg
     1081 ataaaaccat aggctctaat tccattggtg gttcatagag acgatgattt ttggcatatt
     1141 tgactgaagg agtggtcttt cttctataaa taaaatagaa gcagacactc aataacaagg
     1201 agatagaaag tatcgaaggg aggacccaag taacgagttg tttactctga tctttttctc
     1261 taacaatcga gtcttctatc ttattaaact cttctaaacg attccctttc aagccctgat
     1321 ccctagcgct agcaaaatcg gtccgagacc aataggcatg caactcaact ccacgcttag
     1381 ccggaagatt gtctaaacgg atagtataat caaggttact cttttcaatc gttccctctc
     1441 taaaaagttt ccctgtatgg aaaaagagtt tttcagcccc cttgtctccc cttacatgaa
     1501 attcaaactt ttcaatagac tctgaactat ctgtcagagg ttgccaattt aattcagcga
     1561 tatcatcata aaggaaaagt aaatttttta agttccagac gaggtcaact tcaactatgt
     1621 cgccctcctg acctggatta tagactctca cagtataacc atccgcttct tctgttactt
     1681 cgctagtcac atctgctagt tctgcaccgt ttttcgcagc ctgaaccttt ggatgagggt
     1741 caatgtcaaa cccgctaggc atcttaccag cacgtccaag tcccacgatt tggcccttaa
     1801 agtcctcctc aaactggtaa actatcttct gtctaaactc tgccgtatta tctgcatgaa
     1861 tatacaaatc cccttgataa gagtttatct tgaaatcaat ggcaaaaaca gagagtggaa
     1921 gaaggcaaaa caagcctaac accagtaaga aaaaagtttt tttcatcaac taagccccct
     1981 ttttatcttt cttatcatta tagcatcttt tcgtaagtaa aggcttactt atgttgaaaa
     2041 attacgcatt tttcgataaa ataggagaca gttatttttt aatagatcag aataggagaa
     2101 atcatgagaa aaatatactt atctattttc acaagtctct tgctgatgct aggacttgtc
     2161 aatgttgctc aagccgatga atatttacgc atcgggatgg aagcagcata tgctcccttt
     2221 aactggaccc aggatgatga tagcaacgga gctgtcaaaa tcgatgggac caatcagtat
     2281 gccaacggat acgatgttca aatcgccaag aaaatcgcta aggacttagg taaagaacct
     2341 ttggttgtta aaaccaagtg ggaaggtcta gtccctgccc ttacttctgg taagattgac
     2401 atgattatcg caggtatgag tccaactgca gaacgcaaac aagaaattgc cttttcgagc
     2461 agttactaca ctagcgaacc agttttgctt gtcaaaaaag attctgccta cgcaagtgct
     2521 aaatctttgg atgactttaa tggtgcgaaa atcacttctc aacaaggtgt ctacctctac
     2581 gacttgattg cacaaatccc aggtgctaaa aaagaaacag ccatgggaga cttcgctcaa
     2641 atgcgccaag ctcttgaggc tggtgtcatt gatgcttatg tttctgaacg tccagaagca
     2701 ctgactgctg aagctgcgaa ctctaagttc aagatgattc aagtagaacc tggtttcaaa
     2761 actggggaag aagatacagc tatcgctatc gggcttcgta aaaatgacaa tcgtattagc
     2821 caaatcaatg ccagcattga aaccatttca aaagatgacc aagttgcctt gatggatcgt
     2881 atgatcaagg aacaacctgc cgaagctaca acaactgaag agactagcag tagtttcttt
     2941 agccaagtcg ctaaaattct ttctgaaaac tggcaacaac tcttgcgtgg tgctggtatc
     3001 actcttttaa tctctatcgt cggaaccatc ataggtctca ttattggact tgccattggt
     3061 gtcttccgta ctgctcctct ctctgaaaac aaagccattt atggccttca aaaactagtc
     3121 ggctgggttc tcaatgtcta cattgaaatt ttccgtggta cgccaatgat tgttcaatcg
     3181 atggttatct actatggaac tgcccaagct ttcgggatca accttgaccg tacactggct
     3241 gctatcttca tcgtttcaat caataccggt gcctacatga ctgaaatcgt ccgtggtggt
     3301 atcctagcag ttgacaaggg acaatttgaa gctgcgactg ctcttggtat gacccataac
     3361 cagaccatgc gtaagattgc cctacctcag gtagtccgta acatcctacc tgcaactggt
     3421 aatgaatttg tcatcaatat caaagataca tctgtattga acgttatctc tgttgtcgaa
     3481 ctttatttct caggaaatac cgtggcaaca caaacctatc aatacttcca gacatttaca
     3541 atcatcgccg tgatttactt tgtcctcacc ttcaccgtaa cacgtatcct acgatttatc
     3601 gagcgccgaa tggacatgga tacctatact acaggtgcta accaaatgca aacggaggat
     3661 ttgaaataat gacacaagca atccttgaaa ttaaacacct caaaaaatcc tatggacaaa
     3721 acgaagtgct aaaagacatt tcactcactg tccacaaggg agaggtcatc tctatcatcg
     3781 gaagctctgg aagcggaaaa tcgaccttcc tacgctccat taacctactt gaaacaccaa
     3841 ctgatggaca aatcctttat catggacaaa acgtcctcga aaaaggctat gacctcacgc
     3901 aataccgtga aaagttgggg atggttttcc aatcctttaa cctctttgaa aatctcaatg
     3961 ttcttgaaaa cacaatcgtc gctcagacaa ctgtcctaaa acgcgaacgc acagaagctg
     4021 aaaagattgc caaagaaaac ctggaaaagg tcggcatggg agaacgctac tggcaagcca
     4081 aaccaaaaca actctcaggt ggtcaaaaac aacgtgtggc catcgctcgt gccctctcca
     4141 tgaatccgga cgctattctc tttgatgaac caacatcagc tctcgatcca gaaatggttg
     4201 gagaagtcct caaaatcatg caggacctgg ctcaggaagg cttgaccatg attgtcgtaa
     4261 cccatgaaat ggaatttgcc cgtgatgtct ctcaccgtgt tatctttatg gataagggcg
     4321 tgatcgctga agaaggtaaa ccagaagacc tcttcaccaa tcctaaagaa gaccgaacaa
     4381 aagagttcct tcaacgctat ctcaaataaa aagaaaaggc tgcatcaacc gtgcagtctt
     4441 tttgctgtcc tcaaaatgaa aaggcagatt ctctatctct actacttgaa ggtcaagaat
     4501 ccgccgttat ccaaagatta atcttcaaat ttttcataat ttttttcata gaaatcaatt
     4561 aagcctaagg ctgtttcaaa tgaaatattt tttacttttg cacgaccctg cgccagagca
     4621 atgatagaca tttcacgcgc attcgtttct ttagagatac ggtagcctgt gattttctta
     4681 tcacgaaccc agccaacaac tgattctact ttttcaaagt ttgacttagc catgtttttc
     4741 tcctattttc ttctttataa tatttaattg tatacgtttt tactactttt gtcaataaaa
     4801 aaaacatata ttcaacgaaa ataattagtt cagaattttc ttacttcctt tttaaatccg
     4861 ataatatata ggttttgcta gcctctacac cataaatatt tgctaataaa actactgtat
     4921 tattaccctc ttaaggtgcg aaattattca agttcttaaa aaatgcttct ctatttccgt
     4981 aagatagctt atttcttcga tttaagaact gaagatgata gttctaacct ttagaaatcc
     5041 aagccttcac tagctcaaaa actcctatct ttcttcctca ataaatcatc tgagagatag
     5101 gtattcctta tatccttcaa attcatcact cctttggata ttgaggtgct agcctagaag
     5161 cagacacccg attatctaaa ccatccttaa tagagatgac aagacagata agctcagtta
     5221 attacttata tatcttttta atatggtata tttgtcaaca aaaagaaatc aaatcatacc
     5281 ttatactcaa tgaaaatcaa agagcaaact aggaagctag ccgcaagttg ctcaaaacac
     5341 tgttttgagg ttgcagatag agctaacgtg gtttgaagag attttcgaag agtattaaag
     5401 acagtgtgac tatgcttgtc aatagatagg aaatcaaaat gaaggatgga aatctgagcc
     5461 aaacatcata agtgctataa tgaacagaag taagaattgc acgataagtt ttctaaaacc
     5521 ataggaatcg tcccctttct tcatcttcat tatagcatat atacatcagt tgtacaaata
     5581 atatgatatt tttttattag tcctcagaca agcatattat aaagcgtttc attaccattt
     5641 aagttaatat aagctggatc aaaaccttcc attctacgaa tcaaacctgc ataatcatgc
     5701 ttatctgcta aagcgatccc aatcaatact gggcctgttc ccttgctagc tcgtttgata
     5761 tactcaaaac gtgtgatatc atcatttggc cccaggatat catttacaaa ctcacgcaaa
     5821 gctcctggac gttgtgggaa attgaccaca aaataatgtt tgataccatc ataaatcaag
     5881 gcacgctctt ccatttctgg catacggttg atatcattat ttcctccaga aatgatacaa
     5941 caaatggttt tccccttaat atattcagct aaaacctcta aagaggcgat actagctgct
     6001 ccagcaggtt ctgcgactat cccttgctta gagtaaaggt caatcaaggt ttcagaaatc
     6061 aatccctcat cgacacctac caaagtttga atatgttgac gagttgcttc ataggtcaac
     6121 tgacctacct tttgcacagc aatcccatcc gcaaatttat caatttcctt gagtttgact
     6181 ggacctccag cctcaaaggc agctttcatg gaacgcgctc cattcgcctc tactccgata
     6241 acctcaatct ctggacttgt ttccttgata taggtagaaa ccccggcaat gagaccgcca
     6301 ccaccaacag gaaccaagac agcatcaaaa tcaatcgatt cttttcgagc ttcttctaaa
     6361 atctcataag caactgttcc ttgacctgct tgaacatgag catcatcaaa aggatcaata
     6421 aaggtacgat tttcagagac tgtaaattct tgagctgctt tggctgaggc atcaaaggta
     6481 tctccaacta gtttaatagt tacaaaatcc ccaccaaaaa agcgaacctg accaatcttt
     6541 tgttgtggcg tagtaatagg cataaagata gtagcaggaa ttttcatttc attacaagta
     6601 taggctactc cctgcgcatg atttcccgca gaagcgcaaa ctaccccacg ttcacgttct
     6661 tccttgctga gctgggaaat ggcataatag gcaccacgaa ttttaaagga gcgaacacgc
     6721 tgggcatttt cttttttcaa ataaatctta gcaccatact tctccgataa ataatgatcg
     6781 taatccagtg gagtattcac aaccacaccg ttcaagacct tgtgagcctt gattatatct
     6841 tttgaactta acatataaaa cctctttttc tataaaaaac agtcactaat gactgttttt
     6901 tagttattca tctccgaaaa tttcagtaac actttcatgg tatttatgag ccatacgaat
     6961 cgaaaactct ttaggaagtt cataacgatt tgtggaaatc caatcatata accattctac
     7021 caaactttct tcactatcat tacgatggaa gataaaatag tttaaaattt ctttatattc
     7081 tacatcattc aaatccaacg tgtctacaaa ttgactacga ggaacaaaat tagaatctaa
     7141 ttttcttagt tgatctgtca attctcccaa ttgtaaatcc aactgaaaaa atagtttttg
     7201 cagttgccta tcagtttcat cacatcctgt cttgatagaa aacaaattgg taatattcat
     7261 atgatttcaa cccctaaata agtacatcta atatcccacg aatagctgaa gctaaagttt
     7321 tcgcagtcca gtcatttaaa cccgattctt tcaatccaga tgcaataatt tgctctattt
     7381 gatataaacc gccatctatc gtctcattta ttctgcgaag aggaccttca atataggata
     7441 aagcttctcc gaatctaaca gctgatttag gtcctaatga tttaccaact atatttgtta
     7501 atatttttta tttctcaaaa tccatttcgc tatataacga gcagatttag tcgcaccagc
     7561 tctctcaaac ggagaatgaa tttcaagacc ttcttctcta gctatttgtg ccaatttgaa
     7621 gaattgttca aaggtcatat gcaacctacc atcttcaaaa ttagcatcat cataaatgtt
     7681 aattacagca ttttcatcat acgaccaatc tgctttattt tggtctgcta gcactattac
     7741 cgacggtgta attacattat acaactagaa ccaataataa cgatgcaatt tttctcataa
     7801 tctttttcat aaaatcatct ccagcgctat atatttctaa ttagttatag attttgaatg
     7861 catcatcgtc gtttttacca acgaatggca ttgctttacg caattctgca ccaacttttt
     7921 caatttcaag gttagctgct tgttcacggt aagcagtcaa ttttggacgt ccagctttat
     7981 agtcatttac aaagtcattt gcaaatttac cattttggat gtctgccaag acagccttca
     8041 tattttcttt aacttgttca gtgattacac gtggacctga tacatagtca ccgtattcag
     8101 cagtgtttga aatagattga cgcattttct tgaatccacc ttcgtagatc aagtcaacga
     8161 tcaatttcat ttcgtgaaga acttcaaagt aagccaattc tggagcgtaa cctgcttctg
     8221 tcaagacttc gaaacctgct tcgataaggg cagtcaaacc accacaaagt acagcttgtt
     8281 caccaaacaa atcttcttca gtttcttctt tgtaagttgt ttcaagaaga cctacacgag
     8341 ccgctccaac acctttacac cagtccatag caatgttttt agcatttcct gttgcatctt
     8401 ggtatactgc ataaagagct ggaacaccaa atccttcttc gtaagtacga cgtaccaagt
     8461 gtcctggtcc tttaggagca cacatgaaga catctacatc cgcaggaact ttgataaatt
     8521 caaagtggat gttgaaacca tgggcaaatc caactgcgtt tccagcttcc aagtttggag
     8581 cgatttctgc ttcgtacaat tcttgttgaa tttcgtctgg cgccaagatc atgataacat
     8641 cagccaactt agtagcttct gctactgtgt aagtatcaaa tccatcttct tttgctttat
     8701 caaaagattt acctggacgt acaccgataa taacgtcacg acctgaatca cgcaagtttt
     8761 gagcatgcgc atgcccttgt gaaccataac cgataacggc gattttttta ccgtcaagtg
     8821 ctgctacttt aacatctttt tcatattcca tttgaactgt catagctttt ctctcttttc
     8881 tattatttat tgccttttag gctggtttaa caaatttaag ttggattttt aatcgcgggt
     8941 aaatccagtt gcacccgttc gagcaatatt gcgaataccg tatgggcgaa tgactctcaa
     9001 tagggcttcg ctcttttctg catttcccgt catctgaatg gtaatcgagc ttggcgctac
     9061 gtctaccact gttgcacgga aaggttgaat aatcgctaaa atctcagctc tcttctcagc
     9121 tggcgctgac atcttaacca aaatcacctc gcgctccaaa tgaggcttgt ctgtaatatc
     9181 tcgaatgcga atcacatcaa tctgacgatt gagctgtttg atgatttgct ccacttcatc
     9241 atgagaagca acatcaataa taatagtgat acgcgataca ttcggatctt ctgttgctcc
     9301 aacagagatg ctttcaatat taacctgacg acgagatagg acacctgtaa agcgattgag
     9361 gactcctgaa cgattttgta gttttgctgt taacattcta cgcatggaac tgcaccccca
     9421 acatctcatg attactctta ccagccggta ccattggtaa cacctgttcc ttacgagaaa
     9481 tatctacctc aattagcata ggaacatcct cagtgatgac ttcaaggtct tgagccaagg
     9541 tctcaggatt gtcaaactta tagtttttaa taccataagc ctgcgccatc aattggaaat
     9601 caggaaggat atcaaagacc gactctgatg ttctgccttc atagaaggat tcctgccact
     9661 ggcgaaccat tccgagtgaa tgattgttca gcataaccac cttgattggc accttgtaaa
     9721 tattcaaaat agccaactcc tggttagtca tttggaaacc accatcccca acaaacaaga
     9781 ctacttcctt atctgggtta gcaattttag caccgattgc tgctggaatt ccaaagccca
     9841 ttgttcccaa accacctgaa gtcactaact gacgttcatt ttggtaggga taatactgag
     9901 ctgtccacat ttggtgttga ccaacgtctg ttaccacaat ggcatctcca ttcgtcaatt
     9961 caccaattcg ttcaataact gcttgcggtt gaaccacacg ctctttcttg tcataagaac
    10021 gaacacgatt cttgtcttta gtgactttct caatccactt ttcagtattg ttgtgaactg
    10081 ttggttctgc tagcaacatt tgcaaggcct tcttagcatc tccaactaca ggaatgtctg
    10141 cactgataat cttgccaatc tcagctgggt caatatcaat gtgggcaacc ttagcattct
    10201 tagcgaaagt cttaggattc cccgtcaaac ggtcatcgaa acgagaacca atactaatca
    10261 taaagtccgc ttccgtcatg gcaatatttg ctgcgaatga cccgtgcatg cctcccattc
    10321 caagaaagag tgggtgactc gttgcaatcg ttccttgtcc caaaagactg gttaccactg
    10381 gaatttgata gcgttctgca aattcattta gttccgtagc agcctcagca taactaattc
    10441 caccaccagc taacaagact ggctttttag ccttggacaa ttgcttcaag attttcttga
    10501 tttgcatatc attcggctca agagtcggct gataacttgg taaattcact tctggtgaat
    10561 aaatgaagtc tgtttctaaa gcagatatgt cttttggtag gtcaattaca actggccctg
    10621 gacggcctgt agttgcgata tggacagctt ccgtaatgat acgcggaata tcagctgtct
    10681 cacgaacttg gtaattgtac ttagtgattg gcatggtaat tcccacgatg tctgcctcct
    10741 gaaaggcatc cttcccaatc cctgctcgcg ccacctgacc tgtaaagacc aaaaggggaa
    10801 cgctatcgct catggcatcc gcaatccctg taatggcatt tgttgctcct ggtccactag
    10861 tgacgacggc aacacccaac tttccagttg atttggcata accttcagct tcatgcaaac
    10921 aaccttgctc atggcgccct agaatgtggc gaatgccttt aaaattatat atcgcatcat
    10981 aaaaaggcaa aaccgcacca ccaggataac caaagatggt atcaactcct aaatcacgaa
    11041 gtgtttccaa aactaggtcc gaccccgtct taggagattc taaactgatt ttctccatta
    11101 ttcccctttc tttcctctta aaaataactt gttactatca taccattttt tcaaaatttt
    11161 tcaagataaa agaagaaatt ttctgaattt tctattttaa cgtttattta tgaatgttat
    11221 ttttgttttt attaaaaaga taccgatttc atttactaaa aagaaaaaag aactgatttc
    11281 tcagtccttc attaatctta ttccacacta aataggtatg ggtaaacagg ttgttgacct
    11341 tggtgaatct cgacttcaac gtcttcgaat tcttctacga tttcttgagc gatttcattg
    11401 gcaagttctt cgcttccgtc ttcaccgaca tagaaggtta cgatttcact atcttcatcc
    11461 aacatatgtt tcaaggtttc agtcaatgtt tggtgcatat cagggtttga cacaagaatt
    11521 tttccatcca ccatacctag attatcgttt tcatggattt ctaagccatc gatcgttgta
    11581 tcacgcacgg ctgttgtgac gcttccgcta acgacatcgc taagagcagc tgtcatacgc
    11641 tcttggtttt cttcaatgga cttgcttgga tcaaaggcaa gaagacttgt cataccttga
    11701 ggaagagtgc gagcctctac cactactgct ggttgctcca aaacttctgc cgcagattga
    11761 gctgccatga agatgttctt gttgtttggc aagaagatga tgttacgggc attgacctgt
    11821 tcaacagcct tgataaagtc ttctgttgaa gggttcatgg tttgaccgcc ttcgataaca
    11881 taatccacgc cttgagaaca gaagatatct gctagacctt taccagccac cacagcaatc
    11941 aaagcatact ctttttcttc agccgacttg ataacttgag tagcttcttt ctcaacctgt
    12001 gcttcgtgtt ggttacgcat attgtcaact tttaccttga ccaagctacc atatttgaga
    12061 ccttcttgca taacaagtcc tggatcttct gtatgaacat ggactttgac aatttcatca
    12121 tcgttaacaa cgaggagaga atccccaagc tcatccaagt agttacggaa ttcatcgtag
    12181 tcaaaatctt tagcataggt tggaccttgc ttaagagcta ccatgatttc agtacagtaa
    12241 ccaaacgtga tgtcctcagt tgctacgtga ccagctacag acttatgatg ctctacattg
    12301 atcatctcac tcatgttggc aggagtcgct acaaagtcct cagatgcaat atattcgcca
    12361 gtaagggctg aaaggaaacc ttcgtagatg aagaccaatc cttgaccacc tgagtccaca
    12421 acgccaactt ctttcaatac tggaagcatg tctggtgttt tagctagagc tgttttagca
    12481 ccttccaagg ctgcgcgcat gacttcaaca gtgtcatctg tttgctcagc ttttttctta
    12541 gcaccgatag cagctccacg agaaactgtc aaaatcgttc cttcaacagg tttcatcact
    12601 gccttatagg caacttccac acctgattgg aaggccagag ccaagtcttg acctgttaac
    12661 tcgtctttat ccttgatagc ttgggaaaat ccacggaaaa gctgagacgt aatcactcct
    12721 gagttcccac gcgcacccat caaaagccct ttggcaagaa tgctcgctac ttctccaact
    12781 gtagaagctg gcttgtctgc aacttcttta gcaccatttt caatggtcat tcccatattt
    12841 gtcccagtat ctccatctgg aactggaaag acgtttaatg aattgacata ttcagcttgc
    12901 ttattcaagc gagttgatgc agcctgcacc atttcttgaa ataagctagt agtaattttt
    12961 gacacggtta ttctcctaca actttgatat tttgaatgta gacatttaca gtctgagcag
    13021 taattccaag ctggttttcc aagctaaagg caacacgctc ttgaatgttt tttgacactt
    13081 cactaatctt tgttccgtag cttaacacgg tatatacatc aactgcaata ctgccatctt
    13141 cggctgcctt tacgacgaca cctttagaat aattttcctt acctagcagg gcttggaaat
    13201 tatctttgag ggcattttta ctagccatac cgaccacacc aaaaatctca gttgctgctc
    13261 cacctacgac ggttgcaatc acttcatctg ttagttcgat ttgaccatct tttgtattaa
    13321 tttttacagt catccttttt acctcaacta gttgatactc tattttatca tatttcagcc
    13381 caagtgtaaa agcagaatga tgtatcagcg gatatttact ctatttttca aacgatgtta
    13441 tacccacaat aaaagaaaaa agaccctaag gtctcctttg cttttattat taaacgcgtt
    13501 caactttacc tgatttcaaa gcacgagctg aagcccaaac ttttttaggt ttaccatcga
    13561 taagaacagt aactttttga aggtttggtt ttacggcacg ttttgtttgg ttcatcgcgt
    13621 gtgaacggtt gtttcctgat acagtcttac gacctgtaaa gtaacatact ttagccattg
    13681 tgttttcctc ctattagatc taatatagcg gatgtgctag caccacatac cgtactatgt
    13741 tatcacactt tcttgttttt tgcaagggaa ttggaagatt ttttatttgt gtcttaaatc
    13801 aggtcttgcg tgacattttc tgctctccac atgccatcgt tgattaacag aacaccagaa
    13861 ttaaaattat gtgtataaaa atcatctcta actgcagcta agggtatagc cgtcaagtcc
    13921 aaatcccaca gctcatctat cgattttctt acaacaatat ctgaatccaa atacagtaca
    13981 cgagactcgc ttacatactt tggaataaaa tacctaaaaa agccgcatat gaaagtccct
    14041 caaaggggag acgataacct ttcagaatat tactgtcaat ctaaacattc acaatctcac
    14101 tattcaaagt ctctagtctt ttttccatca attggaacca ttctcgcgga aggtcatcat
    14161 taaaaacata aaacttaaga ttataatgat gaacacaaag agattttatt gttgtttcaa
    14221 ctttatccat ataagcatta tctgcaccta agacaatcgc ttttttctct tttttctctt
    14281 ctttcacttt ttatctcatt tctttttatt cccatcatat gtttctacgt aaccattatt
    14341 ttcgcctatt cgttcgtaaa accataccag tggagatttt agatgaagtc ccattacggt
    14401 ttacaatttt tacattacga cacggagttt tacaaatcga tttcatttgc caaacgtagt
    14461 tagtgaggca gttagctagt tcgccaaata gcgactagcg tccaacaatt tggaacttta
    14521 gttccaattg ttggtactga gtcacatctt ctcctctaac tctacgtctg gatacttgtc
    14581 agcaaaccag cggagggcaa agtcattttc aaagagaaag actggttggt caaaacggtc
    14641 tttggctaag atattgcgac ttgacgacat ccgttcatcc aagtcctcag gcttgatcca
    14701 acgaacggtc tttttaccca ttgggttcat aactacttct gcattgtact cgccttccat
    14761 gcggtgttta aagacttcaa actggagttg acctacagcg cctagcatgt actcacctgt
    14821 ttggtaattc ttataaagct gaacggctcc ttcttgcacc aattgctcaa tccccttgtg
    14881 gaaggatttt tgcttcataa cattcttagc agaaactttc atgaaaattt caggagtaaa
    14941 ggttggcagt ggttcaaatt caaacttgtt tttaccaact gtcaaggtat ccccaacttg
    15001 ataagtaccg gtatcgtaaa ccccgataat atcacctgcc acggcattga tcacattctc
    15061 acgactttcc gccataaact gggtaacatt agatagtttg gcccccttac cagtacgagg
    15121 gagattgaca ctcatgccac gctcaaattc gcctgatacg atacggacaa aggcaatacg
    15181 gtcacggtga cgaggatcca tgttggcttg gattttaaag acaaagcctg agaaatcctt
    15241 gtcataagga tccacaattt caccgtctgt tttcttgtga ccatgtggtt ctggagcaaa
    15301 cttgaggaag atttcaagga aggtctgcac accaaagttt gtcagggctg aaccgaaaaa
    15361 gacaggcgtc aattctccag ccagaatagc ttcctctgaa aactcattcc cagcttcatt
    15421 taaaagctca atgtcatcct tgacttgctc gtagaaagga ttgctaccaa agagtttgtc
    15481 cccgtcttct agactggcaa aacgctcatc ccctttgtaa agctctaaac gttggttata
    15541 gaggtcatac aagccctcaa aggctttccc catcccgata ggccagttca tagggtagct
    15601 agcaatgccc aagatttctt ccaattcttg caagagatcc aaaggctcac gaccgtcacg
    15661 gtccagcttg ttcataaagg taaaaactgg aatgccacga tgtttcacaa cctcaaacaa
    15721 tttcttggtt tgagcctcga tccccttggc agagtccacg accatgaccg cagcatccac
    15781 cgccatcaag gtacgatagg tatcttctga gaagtcctcg tgccctggcg tgtctaagat
    15841 attcacgcgc ttgccgtcgt agtcaaattg cataacagat gaagtaacag aaatcccacg
    15901 ttgcttctcg atatccatcc agtcagattt agcaaaagtc cctgttttct tcccttttac
    15961 cgtaccagcc tcacgaatct cacccccaaa gtagagtaac tgctcagtga tggttgtttt
    16021 ccccgcgtcc gggtgggaga taatggcaaa ggtacgacgt ttcttaattt cttcttgaat
    16081 attcataagt tctctttctt tgattctcta tttttcttgt ttcaatagct gagaatgatt
    16141 tttacattgg attttaccat tcctttcaac attccattat atcggatttt agcatttttt
    16201 tcaatttcta tttcttttca cttccccctc ccttatttat aggaaaatat ggtaaaatag
    16261 aacagactaa aaatcatcat ttcacgaaag gatgcaagat gaaaattacg caagaagagg
    16321 taacacacgt tgccaatctt tcaaaattaa gattctctga agaagaaact gctgcctttg
    16381 cgaccacctt gtctaagatt gttgacatgg ttgaattgct gggcgaagtt gacacaactg
    16441 gtgtcgcacc tactacgact atggctgacc gcaagactgt actccgccct gatgtggccg
    16501 aagaaggaac agaccgtgat cgcttgttta aaaacgtacc tgaacaagac aactactata
    16561 tcaaggtgcc agctatccta gacgatggag gagatgccta atgactttta acaataaaac
    16621 tattgaagag ttgcacaatc tccttgtctc taaggaaatt tctgcaacag aattgaccca
    16681 agcaacactt gaaaatatca agtctcgtga ggaagccctc aattcatttg tcaccatcgc
    16741 tgaggagcaa gctcttgttc aagctaaagc cattgatgaa gctggaattg atgctaacaa
    16801 tgtcctttca ggaattccac ttgctgttaa ggataacatc tctacagacg gtattctcac
    16861 aactgctgcc tcaaaaatgc tctacaacta tgagccaatc tttgatgcga cagctgttgc
    16921 caatgcaaaa accaaaggca tgattgtcgt tggaaagacc aacatggacg aatttgctat
    16981 gggtggttcc ggtgaaactt cacactacgg agcaactaaa aacgcttggg accacagcaa
    17041 ggttcctggt gggtcatcaa gtggttctgc cgcagctgta gcctcaggac aagttcgctt
    17101 gtcacttggt tctgatactg gtggttccat ccgccaacct gctgccttca acggaatcgt
    17161 tggtctcaaa ccaacctacg gaacagtttc acgtttcggt ctcattgcct ttggtagctc
    17221 attagaccag attggacctt ttgctcctac tgttaaggaa aatgccctct tgctcaacgc
    17281 tattgccagc gaagatacta aagactctac ttctgctcct gtccgcatcg ccgactttac
    17341 ttcaaaaatc ggccaagaca ttaagggcat gaaaattgct ttgcctaagg aatacctagg
    17401 cgaaggaatt gatccagagg ttaaggaaac aatcttaaac gcggccaaac actttgaaaa
    17461 attgggtgct atcgtcgaag aagtcagcct tcctcactct aaatacggtg ttgccgttta
    17521 ttacatcatc gcttcatcag aagcttcatc aaacttgcaa cgttttgacg gtatccgtta
    17581 cggctaccgc gcagaagatg cgactaatct tgatgaaatc tatgtaaaca gccgtagcca
    17641 aggttttggt gaagaagtga agcgccgtat catgctgggt actttcagtc tttcatcagg
    17701 ttactacgat gcctactaca agaaggctgg acaggtccgt acacttatca ttcaagattt
    17761 cgaaaaagtc ttcgcggatt acgatttgat tttgggtcca actgctccaa gtgttgccta
    17821 tgacttggat tctctcaacc atgacccagt tgccatgtac ttagccgacc tcttgaccat
    17881 acctgtaaac ttggcaggac tgcctggaat ttcgattcct gctggattct ctcaaggtct
    17941 acctgtcgga ctccaattga ttggtcccaa gtactctgag gaaaccattt accaagctgc
    18001 tgctgctttt gaagcaacaa cagactacca caaacaacaa cccgtgattt ttggaggtga
    18061 caactaatga actttgaaac agtcatcgga cttgaagtcc acgtagagct caacaccaat
    18121 tcaaaaatct tctcacctac ttctgcccac tttggaaatg accaaaatgc caacactaac
    18181 gtgattgact ggtctttccc aggagttcta ccagttctca ataaaggggt tgttgatgcc
    18241 ggtatcaagg ctgctcttgc cctcaacatg gacctccaca aaaaaatgca ctttgaccgc
    18301 aagaactact tctaccctga taatcccaaa gcctaccaaa tttctcagtt tgatgagcca
    18361 atcggttata acggctggat tgaagtcgag ctagaagacg gtacgaccaa gaaaattggt
    18421 atcgaacgcg cccacttaga ggaagacgct ggtaaaaaca cccacggtac agacggctac
    18481 tcttacgttg accttaaccg ccaaggggta cctttgattg agattgtatc tgaagccgat
    18541 atgcgttcgc cagaggaggc ctatgcttat ctgacagccc tcaaggaagt tatccagtac
    18601 gctggcattt ctgacgttaa gatggaagaa ggttctatgc gtgtggatgc caacatctcc
    18661 cttcgtcctt atggtcaaga gaaattcggt accaagactg aattgaagaa cctcaactcc
    18721 ttctcaaacg ttcgtaaagg tcttgagtac gaagtccaac gccaggctga aattcttcgc
    18781 tcaggtggtc aaatccgcca agaaacacgc cgttacgatg aagcgaataa agcaaccatc
    18841 ctcatgcgtg tcaaggaagg ggctgctgac taccgctact tcccagaacc agacctaccc
    18901 ctctttgaaa tttctgacga gtggattgag gaaatgcgga ctgagttgcc agagtttcca
    18961 aaagaacgtc gtgcgcgtta tgtatctgac cttggtttat cagactacga tgctagtcag
    19021 ttgactgcta ataaagtcac ttctgacttc tttgaaaaag ctgttgccct aggtggtgat
    19081 gccaaacaag tctctaactg gctccaaggg gaagtcgctc agttcttgaa tgctgaaggt
    19141 aaaacactgg aacaaatcga attgacacca gaaaacttgg ttgaaatgat tgccatcatc
    19201 gaagacggta ctatttcatc taagattgcc aagaaagtct ttgtccatct agctaaaaat
    19261 ggcggtggcg cgcgtgaata cgtggaaaaa gcaggtatgg ttcaaatttc agatccagct
    19321 atcttgatcc caatcatcca ccaagtcttt gccgataacg aagctgctgt tgccgacttc
    19381 aagtcaggca aacgtaacgc cgacaaagcc tttacaggat tccttatgaa ggcaaccaaa
    19441 ggccaagcca acccacaagt tgcccttaaa ctacttgcac aggaattggc gaagttgaaa
    19501 gaaaactaga cagaacaaaa ccagccctaa ggttggtttt ttcttctcta ccaactccca
    19561 ataactattt tggctttatt tccagagtat tttatggtaa aatgaagagt aataatattt
    19621 attaaagagg taaaaacatg attgaagcaa gtaaattaaa agctggtatg acctttgaaa
    19681 cagctgacgg caaattgatt cgcgttttgg aagctagtca ccacaagcca ggtaaaggaa
    19741 acacgatcat gcgtatgaaa ttgcgtgatg tccgtactgg ttctacattt gacacaagct
    19801 accgtccaga ggaaaaattt gaacaagcta ttatcgagac tgtcccagct caatacttgt
    19861 acaaaatgga tgacacagca tacttcatga atacagaaac ttacgaccag tacgaaatcc
    19921 ctgtagtcaa tgttgaaaac gaattgcttt acatccttga aaactctgat gtgaaaatcc
    19981 aattctacgg aactgaagtg atcggtgtca ccgttcctac tactgttgag ttgacagttg
    20041 ctgaaactca accatctatc aaaggtgcta ctgttacagg ttctggtaaa ccagcaacga
    20101 tggaaactgg acttgtcgta aacgttccag acttcatcga agcaggacaa aaactcgtta
    20161 tcaacactgc agaaggaact tacgtttctc gtgcctaatc tctagaaaga ggtcattcta
    20221 tgggaattga agaacaactt ggcgaaatcg ttatcgcccc acgtgtactt gaaaaaatca
    20281 ttgctatcgc tactgcaaag gtagagggtg ttcactcttt ttcaaacaga tcagtgtctg
    20341 ataccctttc aaaactttca ctcggccgtg gcgtttatct taaaaacgtg gacgaagaac
    20401 tcacagcaga tatctatctc taccttgagt acggagtaaa agttcctaag gtagcggttg
    20461 ctatccagaa agctgtcaaa gatgccgtcc gtaatatggc tgatgtagaa ctcgctgcta
    20521 tcaatattca cgttgcaggt atcgtcccag ataaaacacc aaaaccagaa ttgaaagatc
    20581 tatttgacga ggacttcctc aatgactagt ccactattag aatctagacg ccaactccgt
    20641 aaatgcgctt ttcaagctct catgagcctt gagttcggta cgaatgtcga aactgcttgt
    20701 cgtttcgcct atactcatga tcgtgaagat acggatgtac aacttccagc ctttttgata
    20761 gacctcgttt ctggtgttca agctaaaaag gaagaactag ataagcaaat cactcagcat
    20821 ttaaaagtag gttggaccat tgaacgctta acgctcgtgg agagaaacct ccttcgcttg
    20881 ggagtttttg aaatcacttc atttgacact cctcagctgg ttgctgttaa tgaagctatc
    20941 gagcttgcaa aggacttctc cgatcaaaaa tctgcccgtt ttatcaatgg actgctcagc
    21001 cagtttgtaa cagaagaaca ataaaaagaa aaagtgtttg atagatatca aacacttttt
    21061 ctttgcctcc cactatctaa aaaaatgata atagatataa ttgtaaacaa aaatccagat
    21121 aggttttgca tgattgagaa agttaaaaaa actatggcag agaatcgtta atctcagatt
    21181 gtcggtagaa cgataaacaa gggcaaaaaa gaaaccaatc agactataat ataataaact
    21241 aattggatct ctgtgagata gtatcaaatg gctaatccca aagataaggc ctgataagat
    21301 gacatctaaa tagtactttg acttaggaaa gaaggtattc ataaaatacc ctctatcaag
    21361 agtctcctca aaaacaggac cgatgattac aagcaggaca aaagataaga tagtcgataa
    21421 aaaggttggt tgtccatttg aaagaaccac ggtaaaatac tcatcatgaa tattcctatg
    21481 gttaatcaaa tgagcatagc gtgcctaaaa attaccgaga atctgataaa ccacataagt
    21541 tgcaaataag gagaagacaa atgaccagtc ccagctcttt ttctttttta acctccaaat
    21601 taatagaaaa ggaaacttcc cactaatccc actgttaaaa taagagaata gacatcagct
    21661 cctaacccta aaatgatcgt cacatacaat ccaattgttt gtggtaaata ggtagatagt
    21721 aaaataataa gcaaaaatat tccaaattgt cttagtttct ttgtgtttct catcgtactt
    21781 ttttgaaaga ttaccctgtt cggaagccgt acttccaagc atctatataa gaattaagtg
    21841 ccccttgcct catataggga gcaaattctc tataatataa ccatctacta tatccatctt
    21901 cccaaacagc aagaccacct gaagtttgct ccaagtcctc agttgaaaga actgtaaatg
    21961 tttctgtacc tgtcattgca agtaccttct taaaatagat tgttgtaggc tcacatttat
    22021 agtatatttc tttttttgtc tattttatag tctatctcct caactggcaa tttttcgacc
    22081 tgaattgcat ttttccataa aaaatgagac ctttctagtc tcatttagtc attcttagta
    22141 ttttctaaat cgttgatagc gttcttccag caactcttct agcggttttt gtgaaagtct
    22201 agccagctcc gtttggagtt cttttttgac actcttaatt agttctttac tagaaagtcc
    22261 tacttcagaa atcaccttat ccaccacgtc catttctaac agttcatgcg aagtgatttt
    22321 catcagttct gctgcttcca tggcgcgagt accgtccttc cataaaatgg aagcaaagcc
    22381 ttctggactg agaatggcat agatagaatt ttccagcatc cagacacggt ccgcgacagc
    22441 tagagccaga gccccgcctg aaccaccttc accgataata atggcgataa taggaacttt
    22501 caggtcactc atttccatga gattgcgagc gatagcttcc ccttgaccac gttcttccgc
    22561 tccgacacca ggataagcac ctgctgtatt gataaaggtc acaactggac ggccaaattt
    22621 ctcagcctgt ttcatcaacc gcagtgcctt tcggtagcct tctggatgtg gttggccaaa
    22681 attccgtttg aggttgtctt gcaaactctt gcctttttgg ataccaacca ctgttacagc
    22741 ttggtctcca agccaaccaa taccaccaac aactgcacca tcatcacgaa aagaacggtc
    22801 accatgtaat tggataaatt catcaaaaat gcctgtcgca aagtccaagg ttgtcaagcg
    22861 actctgctca cgcgcttctc tgactatttt tgcaatattc atctaggact ccctccatgc
    22921 aatctgacta ggctagcaat cgtatctggt aagtctcttc ttttgacaat agcatccaca
    22981 aagccatgtt ctaataggaa ttctgccttt tggaaatcct caggcaagct ttcacgaacc
    23041 gtattttcaa tcacacgacg cccagcaaaa ccaaccaagc tctgtggttc agccagaatg
    23101 atatcgcctt ccatagcgaa agaagctgtc acaccaccag tcgttggatc tgtcaaaatg
    23161 gtcaggtaaa agagaccagc atttgaatgg cgtttaaccg ctgcagagat cttagccatc
    23221 tgcatgagac tcatgattcc ttcctgcata cgggctccac cagaggctgt gaataggaca
    23281 actggtaatt tttcgacagt cgcatactca aacaaacgag tgattttttc acctacaacc
    23341 gtacccatag aagccatgat aaaattagaa tccataatcc caagagccac agtctgacct
    23401 ttaataagag cagttcctgt cacaacggct tcatgcagac ctgttttttc acgcatagat
    23461 gccagtttct tttggtaacc agggaaatgc aagggatcct tgctttcaat ccctgtaaac
    23521 aattctttga aggttcccat atcaatcgtc aaagccaagc gttcttgggc agaaatacga
    23581 aaggtatagc tacagtgcgg acagatacgt tcacttccca gatccttctg atagatggta
    23641 tgcttacagc ctggacactg ggaaaataat tcatctggaa cctctggctt agcttgaggt
    23701 ttttccctaa ccgaacgatt gggattgatt cgaatatact tatctttttt actaaataga
    23761 gccattgatt ccccttttcg gtttaaactc ttaaagtcat tttattcttt ttcttgatat
    23821 ttaggtaaga aggtttccat caagaaggaa gtatcataat ccccagcaat gacattgcga
    23881 tctgaaatga gatcaagctg gaaatctgca ttggtctgca ctccttcaat ctctaattca
    23941 tagagggcac gttgcatttt catcaaggcg tcaaaacgat tttcgccgtg tactatgatt
    24001 ttggcaatca tactatcata ataaggcgga atggtataac ctggataaac tgctgaatcc
    24061 acgcgcaagc caactccacc acttggcaga tagagattag taatcttacc tggacttgga
    24121 gcaaagttaa aggctgggtt ttctgcattg atacgacact cgatggcatg accgcgtagg
    24181 acaatatctt cttgcttaac agacaaaggc tgacctgccg caatgcaaat ctgttcctta
    24241 acgatatcaa cacctgaaac aaactctgtt actggatgtt ctacctgaac acgagtattc
    24301 atctccatga aatagaaatt gctacttgct tcatcaagaa gaaattcaat ggttcctgca
    24361 ttctcatagc caacaaactc tgccgctcga acagcagcag cacctatttc atgacgcagc
    24421 gtttttccga ttgcaatcga gggactttct tccaaaacct tttggttatt cctttgaaga
    24481 gaacaatccc gttcacccaa gtgaatcaca tgtccatgct catcacctag gatttgaacc
    24541 tcaatgtgcc gagctggata gataacccgt tctatgtaca tggcaccatt gccataattg
    24601 gccttggcct cactagaggc agtttcaaag gcagaaacga ggtcatctgg tttttcaacc
    24661 ttacgaatcc ctttaccacc tccacctgct gaagccttga gcataacagg atagccaatt
    24721 ttttcagcaa caatcaaagc ttcttcagag ttatgcactt ctccatctga acctggtata
    24781 acaggcacac ctgctttaat catctgagca cgcgcattga tcttatcccc catcatatcc
    24841 ataacatgac cagatggacc gataaacttg atacctactt cttcacacat ggtcgcaaat
    24901 ttggaatttt cactgagaaa tccaaaacca gggtgaatag cttctgcctc agtcaagact
    24961 gcagctgata gaactgcatt aatattgaga taagactctg ttgccttgcc aggaccaata
    25021 caaactgctt catctgccaa cagcgtatga agagcttcct tatcagcagt tgaataaacc
    25081 gctaccgtcg caatccccaa ttcacgtgcc gcacggataa tacgaaccgc aatttcacca
    25141 cgattggcaa ttaaaatttt tcgaaacatg gagaacctcc ttagttccca attgcaaaag
    25201 taagggtacc actggctgca agcttgccat ccacttcagc ctttgcttca accacagcta
    25261 tggtgccacg acgttttaca aaagtcgctg tcataaccaa ttggtcgcct ggtacaactt
    25321 gcttcttgaa cttaacctta tccataccag cgtaaaagac cagttttcct ttattttcag
    25381 gttttgataa ctccaacaca ccagcagttt gcgccaaggc ttccataatc agaacacctg
    25441 gcataactgg gtattgagga aagtggccgt taaagaaagg ctcgttgatg gtcacatttt
    25501 tgatagcaac aatggtatcc tcgctcacct ccaagacacg gtccactaga agcataggat
    25561 aacggtgggg aagggcttct ttgattcctt gaatatcgat catttgatac gtaccaatcc
    25621 tttaccaaac tcaaccattt cttcgttaga gacgagaatt tccgttacca caccatcctt
    25681 aggagctggg atttcattca tgactttcat ggcttcgata attaccaatg tttgaccttt
    25741 tttgacacta tcaccaactg taacgaaggc aggtttatct ggtccagcag ccaagtaaac
    25801 cactccaaca agtggactct ctacaagatt tccctcacta gccacacttg cttcagctgg
    25861 agctggaact tcttctgcta cagtctctgc tggagcagat gtaggagcta ctggactcgg
    25921 tgttgctaga acgggtgctg gagcgacttg agttgcaact tcaggcacag gtctcgcttc
    25981 attcttgcta aactgcaact catccgtccc atttttataa gaaaattctc tcaaacttga
    26041 ctggtcaaat tgagtcatca agtctttaat atcgtttaaa ttcatactta tctattctcc
    26101 caacgtttga aagcaagaac tgcattgtgg cctccaaaac caaaagtatt tgaaatagcg
    26161 tatggaattt ctttctccaa gccttgtcca taaacgacat tagcttcgat ataatctgat
    26221 acttcacttg tcccagctgt cattggtaca aagttatgac gcatagcttc gatggtgacg
    26281 atagcttcta ctgcacccgc agcccccagc aaatgtcctg taaaagactt ggttgatgat
    26341 acaggtactt ccttaccaag aacagctacg atagcaccac tttctccttt ttcattggca
    26401 ggagttgacg ttccgtgagc attgacatag gctacttgct ctggagaaat ctcagcttct
    26461 tccaaggcta gtttgatggc cttgatagct ccctgacctt ctggatgtgg agaagtcatg
    26521 tggtaggcat cacaagtatt tccgtaacca accacttcag ccaggatagt agctccacgt
    26581 ttttcagcgt gttcaagact ttctagaacc aacatccctg aaccttcacc cataacaaac
    26641 ccattgcgat ccttatcaaa tgggatcgaa gcacgagttg gatcctctgt agtagagaga
    26701 gctgttaagg cttggaaacc agcgatggca aaaggtgtga tagaagcttc tgttcctccc
    26761 accaacatca catcttggaa accaaactta atggagcgga aggcatcccc aatcgcatca
    26821 tttgatgaag agcaggcagt attgatagat ttacaaacac cgtttgcacc aaaacgcatg
    26881 gctacattcc cagaagccat atttggtaaa gcttttggaa gagtcattgg tttgacacgt
    26941 ttgggtcctt tttcatgaag gcgaagtacc tgatcttcaa tttccttgat tccaccaata
    27001 ccagatgcaa cgataacacc aaaacgatcc ctattaagag cctctacatc aagattggca
    27061 tgatttacag cctcttgggc tgcatacaag gcatataaag aatagttatc aaaacggttg
    27121 gtatcttttt ttacaaagta tttatcgaac ggaaaatctt ggatttctgc cgcattatgc
    27181 acatcaaagt cactatgatc aaattttgta atgccaccaa tgccgatttt cccagttgct
    27241 aaactattcc aaaattcttc tggtgtattt ccgattggag atgttactcc ataacctgtt
    27301 accactactc gatttagttt cattcttttc acctctagct ttcgctacat acttaagcca
    27361 ccatcaatgg caatcacttg accagttaga taatcttggc ctgctaaaaa tactgtcaaa
    27421 tctgcaacct gctctgcctg cccaaattct ttcatcggaa tctgagctag tgtagcttcc
    27481 ttaatcttat ctgataagat agctgtcata tcagactcaa tcattcctgg agcaatcaca
    27541 ttgactcgta tattccgact agcgacctcg cgtgccacag acttggtaaa gccaatcaag
    27601 ccagccttag aagcagcata gttagcttga ccaatattcc ccatcaaacc aacaacacta
    27661 gacatattaa tgatagcacc ttctctggct ttcatcatcg gtttcaagac tgattgtgtc
    27721 atattaaagg caccagtcag attgaccttg agcacttttt caaaatctgc ttctgtcatc
    27781 ttgagcataa gagtatcttg ggtaatccct gcattgttga ccaaaacatc tactgaaccc
    27841 agttctgcaa tagcttgatc aatcatacgc ttagcgtctg caaaatctga tacatctcct
    27901 gaaatgggaa ccaccttgat accatagttt gaaaactcag cgagcaattc ttctgagatt
    27961 gccccacgac tgtttaagac aatgttggct cctgcttgag caaacttgtg ggcgatggca
    28021 agaccaattc cacgactcga acctgtaata aagatatttt tatgttctag tttcattttt
    28081 ttcctttcaa aacttctact tattttagtc tatttttcta aaagtgctac taaactcgct
    28141 tgatcttcca catgagctaa gtgagcagtt tgatcaattt ttttaacaaa acctgacaag
    28201 actttccccg gtccaatctc gataaagttg cttatgcctg cttcttgcat gaccccaata
    28261 ctttcataga aacgaacggg ttccttgacc tgacgcgtca agagctgagc aatgtcctct
    28321 ttttgcatca cagcagcttc tgtattgccg actaggggac aagtaaaatc tgaaaaactt
    28381 acctgagcta gagtttcagc tagtttctgg ctagcaggtt caaggagagc ggtgtgaaag
    28441 ggacctgaca ccttaagagg aatcaagcgt ttggcacctg cttcttgcaa aagttcaacc
    28501 gctcgatcaa ctgcaaccac ttctccagca atgacgattt gtgcaggtgt gttatagttg
    28561 gctggagtaa ccactccaag ttcagaagct ttttgacagg cttcttcaat gacctctact
    28621 ggcgtattga gaactgctac catcttgcca gagtcagcag gagccgcttc ttccatatag
    28681 gctccacgct tagctaccaa ggcaaccgca tcttcaaaat ccaaggcgcc gcttgccacc
    28741 aaggcagagt attctccaag agacaaacca gcaaccatat caggctgata gcccttttct
    28801 tgcaataaac ggtagatagc aaccgaagtc gctagaatgg ctggttgcgt atagcgggtc
    28861 tgattgagtt tgtcttcttc cgtatcgatg agataacgta aatcataacc gagcacctga
    28921 ctcgctcgat caatcgtttc tttaacaatc ggatactgat catagaaatc ccgtcccatc
    28981 cctagatact gggcaccttg accagcaaat aaaaaggctg ttttagtcat ttcttacaac
    29041 tcctgcccag cgagaggctt cttcttgaat tttcttagcg gctccgtaat acaaatcttt
    29101 taggatttct tcagctgttt cttctttaga aacaagccct gcgatttgac ctgccatgac
    29161 agagccaccc tccacatcac cgtgaacaac tgctttggct agggcacctg ctcccatttg
    29221 ttcaaagatt tctaaatcag gatcttcctg cttaaaggca tctttttcag ccagttcaaa
    29281 atctctagtc aactgatttt taatagcacg aacagcatga ccaaagtgct gagctgaaat
    29341 cgtagtatca atatcccttg cttttaaaat tttctctttg tagtttggat gggcattcga
    29401 ctcttttgca actacaaacc gtgtccccac ctgtacagcc tctgcaccta gcataaagcc
    29461 agccgcagca ccttcaccat ccgcaattcc tcctgcagca ataacaggaa tagatatagc
    29521 tgtggctacc tgtcgcacca aggtcatggt tgttaattta ccgatatgcc ccccagcttc
    29581 cattccttct gcaataacag cgtctgcacc gattttttcc atgcgtttag ctaaagcgac
    29641 actaggaaca acaggaataa cgattatccc agcttcatgg aaacgttcca tatacttgct
    29701 tggatttcct gctcctgttg tgacaacttt aacaccttct tcaataacga gatccacgat
    29761 atcttccaca aagggagata agagcatgat gttgacccca aagggtttat cagtcaatga
    29821 tttgatttta tcaatattgg ccttgacaac ttctttcggg gcatttcccc caccgataat
    29881 tcctaatcct ccagccttgg aaacagcccc tgccaaatca ccatcagcaa cccaggccat
    29941 ccctccttgg aaaataggat agtcaatctt caataattct gtaatacgcg ttttcatagt
    30001 gcctccaacc ttccttgctt acgtaatagt tcgatttcac cataatttga cagtcaaact
    30061 attacctaaa caagagggag tgggtttctc cctactcctt ctactaatat tctgcttatt
    30121 ttgcttgctc ttcaacgtaa gcaaccaagt caccaactgt tttcaagtca ttttctgctt
    30181 cgatttggat atcaaaagca tcttcgattt ctgagattac ttggaacaag tccaatgaat
    30241 ctgcgtccaa atcatcaaaa gttgattcaa gtgttacttc tgatgcgtct tttccaagtt
    30301 cttcaacgat aatttcttgt actttttcaa atactgccat gataggactc ctttaaaata
    30361 aatagttttt ttataacaat gtgttcacca catgattacc taaattgtaa gaatgagcgt
    30421 gccccaggtc aagcctccac cgaagcctga tagaagaaca gtttggctac catctaaagg
    30481 gatgagacct tgttctacac actctgaaag taaaatcggg atactggctg cactggtatt
    30541 gccatattcc atcatattag ctggaagttt ggctcggtca acaccaattt ttctagccat
    30601 cttatccaaa atacggttat tggcttgatg aagtagcaga taatccaagt ctgtcacctc
    30661 tataggagat tcatcaatag tctgcttgat agacttggct acatctcgaa tggcaaaatc
    30721 aaagactgtg cgtccatcca tcttcaaaaa tgaatctgca ctttcttgat ctgaaaatgg
    30781 agaatgcaaa cctgaatgcc cataagttaa acactcgctg cgacttccat cgctattgag
    30841 actctcagct aagaaatgct cttgctcgct agcttctaac aagacaccac cagcaccatc
    30901 tccaaacaac acagctgttg atcgatccga ccaatcgact gccttagaga gggtttcact
    30961 accaatcacc aagccttttt gaaagcgacc agaagcgata aacttttcag cagttgaaag
    31021 agcaaataca aatccactgc aagccgcggt taagtcaaaa gcaaaggctt tattagcacc
    31081 aatattagct tgaacacgag cagctgtaga gggcatcatc gaatctggag taatggtagc
    31141 taggatgata aaatccagtt cttctcctgt tattccagct tttgccatca gtttcttagc
    31201 aacctctgta gccaaatcac tggtagattc tgttcttgaa atatgccttt gtcgtattcc
    31261 cgttcgactt gaaatccact catcattggt atccataatc tgagccaagt cgtgatttgt
    31321 aaccacttgc tctggcacat aatgagcaac ctgacttatt tttgcaaaag ccattatttc
    31381 aaatcctcca aaaattggta aagattagtc aaacctttac ccatgacagc aatttcttcc
    31441 tcgctcatgc catcaataat tttttctacc atggccttgt ggaagcgttt atgcagtcta
    31501 tgaatcaagc gacccttctt tgtcaaatgc agatgcacca cacgacgatc ctgttctgac
    31561 cgaactcgct caatgtagcc cttacgctct aaattgttca aacttgtcgt aacagttcca
    31621 agagttacca tcaactcttt tgacacttga cttggagtca catctggagc cttaccgatg
    31681 acatcaatcg tatgcatttc tttgatggag atatccttaa aacgactacc tctcaagttc
    31741 acttcctcaa ttacaaggac attgttaaat atagatgtta aatattcatt gattcgttgg
    31801 tagtccattt ttcatatccc tccttcttca aaaaactttt acaatcaaaa catttgacag
    31861 aaagaattat atcaaaattc aaagaatgat gcaagtattt tttattttcc tgtaaattta
    31921 ggtcttcttc tctccgaatg agctcgcact ccttctttga aatcctctgt ttgagctaag
    31981 gatttctgta ggttcagttc taaagtagcg taaccctgcc agtctttaaa ttggctctcc
    32041 caaaccaact tcttaatggc agcataggaa ttacttgaag cacgtcttaa ttttttaaga
    32101 agctgttctc tcgtcttttc aagtttttca gcttcagaaa cgcggtaaac caacccccat
    32161 tctagggctt tttctgccgt taaagcctct cctgtcatag ctaattgagc agcacgcgtc
    32221 acaccgatac tgcggctcaa gagatgaatt ccccctgcat ctggagccaa accaacacca
    32281 acaaaggctt ggataaactt agccttatcc gtcgccaaac agaaatctgc cgcaacagcc
    32341 atattcgctg cggcacctgc aacagcaccg tcaacttcca ttaaaacagg tttggctatt
    32401 tgcttgattt tataagaaat cgtattgacc aactctgcga tttttgtcaa tgatggaata
    32461 tcatcctcat ccactgcccg cttcatctct accaaatctc ccccaactga gaagaccttt
    32521 ccattggcat tgattaagat aaaatgcaca gctggatttt cttctgccaa agtcagagct
    32581 tctaaaatct cctcacacat gggaatatga aaaccatttg cgacctcagg acggttcaag
    32641 gtaaggattg ccaagtcctc ttcaagctga taaataatgt gttccatcag ggctcctttt
    32701 tattttataa gcagtttata ctcttcgaaa atctctttaa accgcgtcag cttccatctg
    32761 caacctcaaa acagtatttt gagctgactt cgtctagctt cttagtttgc tctttgattt
    32821 tcattgagtg ttaaattatt tcactgtcaa agtatatctt ttttaggagc agaaagcaag
    32881 aaaaaatcga ctgaaagtgt ctcagccgat aagaaaggct cgctttaggc tagaaagcat
    32941 cttattttct tcattgaaaa aagcctttct ttctgctata atcgtataaa atacttactt
    33001 taggagttct tatgaaagtt gttaaatttg gaggtagttc tcttgcctct gctagtcaat
    33061 tagaaaaagt tttaaacatc gtcaaaagcg attcagagcg tcgttttgta gtcgtttctg
    33121 cgcctggtaa acgcaatgct gaagatacta aggttacgga tgccctgatt aaatactacc
    33181 gcaactatgt tgcgggtaac gatattagca agagccaaag ctggattatc gaccgctatg
    33241 ctgctatggt tagtgaattg ggactaaaac cagctgtgct agaaaaaatt tctaaaagca
    33301 ttcacgcctt ggccactctt cctattgaag aaaatgaatt tctctacgat actttcctag
    33361 cagccggtga aaataacaat gccaaattga ttgctgccta ctttaaccaa aatggtatcg
    33421 atgcacgcta tatgcaccct agagaagctg ggattgtggt cacaagtgaa cctggtcacg
    33481 ctcgcatcat tccatcaagt tatgacaaga ttgaagaatt gacaaacacc aatgaagtcc
    33541 ttgtcattcc tggtttcttt ggtgtcacta aggaaaatca aatctgtact ttctcacgtg
    33601 gaggttctga tattacaggt tctatcattg ctgctggtgt caaagctgac ctctatgaaa
    33661 actttacgga cgttgatggt atctttgcag cccaccctgg tattatccac caaccacact
    33721 cgattcctga gttgacctac cgtgaaatgc gcgagttggc ctatgcaggc ttctcagtcc
    33781 ttcatgacga ggctcttctt cctgcctacc gtggaaaaat tcctctggtt atcaagaata
    33841 ccaacaaccc tgaccatcca ggtactcgta tcgttctaaa acacagtaat gatgaatttc
    33901 cagttgtggg aattgctggt gactcaggct ttgtcagcat taacatgtcg aaatacctca
    33961 tgaaccgtga ggttggattt ggccgcaagg ttctgcaaat cctggaagaa cttaacatcg
    34021 gttgggaaca tatgccaaca ggtatcgacg atctttctat cattctccgt tctcgccaac
    34081 taactcccat caaggaagaa gaaatcctgc gtcagttggt tcaaaaggct aaagtagatc
    34141 atgcagaaat cgaacacgac ctttctatca ttatgattgt tggtgaaaaa atgaagagcc
    34201 atatcggagt gactgctact gcgacacgcg ctctatctga aaacaaaatc aacatccaga
    34261 tgatgtctca aggttctagt gaagtttcta ttatgtttgt tgtcaataaa gaccaagaga
    34321 aagcagcaat taaagccctc tacaatgcct tttttggaga aagtaaggaa gactaagcat
    34381 ggcccaatca cttaacaaaa cagtactcct cagtacgaca ggcacttcct acctctctat
    34441 cgctgggaaa gttgggaaat tccttgtcgg agatcaggct ttggaatttt acccagatgt
    34501 caatgtcgaa caatttatcc agattccttg gagccatatc aatcagattg gagccaatgt
    34561 cactggacgc aaaatcagcc gccacttcga agtctttaca gacaaaggaa aattcctctt
    34621 tgcttcaaaa gactcaggtg ccattctcaa aattgcacgc gaaaaactag gcaatgacaa
    34681 ggtcgtcaaa cttccgaccc tgattcagac aattagccaa aaatttaaaa atctatttgc
    34741 aaaaaagtag aaactttagt ataatcatag caacggataa gtaggttatc ttcttctttc
    34801 agaaagtctg cgggtactgc gagcagatag gggggatagg tgaaattcta ccgttagtaa
    34861 caattacata cacaatcaag tgcacacctg tgaagttgga tggaaccgtg gccctgccac
    34921 tccaacgttt tgtcaggcgt gcttttttca taaaggagtt cttatgttag atatcaaacg
    34981 tattcgtaca gattttgaag ctgtcgcaga aaaattagct acacgtggtg tagatgctgc
    35041 tgtcttgaat gaaatgaaag aaatcgatgc taaacgtcgt aacatcttgg tcaaggttga
    35101 aactctcaaa gcagaacgta acacagtttc tgctgagatt gcccaagcta agcgcaacaa
    35161 ggaaaataca gatgacaaga ttgctgccat gcaaaatcta tctgctgagg ttaaagcctt
    35221 ggatgctgaa ttggcagaaa tcgatgctaa attgacagaa tttacaacga ctcttccaaa
    35281 tatcccagct gacagcgttc ctgttgggac tgacgaagac gacaatgtgg aagttcgccg
    35341 ttggggtact ccacgcgagt ttgacttcga acctaaagct cactgggatc tcggtgaaga
    35401 ccttggtatc cttgactggg aacgcggtgg taaggtaaca ggcgctcgct tcctcttcta
    35461 taaaggcctc ggtgctcgtt tggaacgtgc tatctacaac tttatgttgg atgaacatgg
    35521 aaaagaaggc tatactgaag tcatcacacc ttacatagtc aaccatgatt ctatgtttgg
    35581 tactggtcag tatccaaaat ttaaggaaga tacttttgaa ctcagcgata ccaactttgt
    35641 cttgattcca actgctgaag ttcctctgac aaactactac cgtgatgaaa tcttagacgg
    35701 caaagatctt ccaatctact tcactgccat gagtccgtca ttccgttctg aggctggttc
    35761 tgccggtcgt gatacgcgtg gcttgatccg tttgcaccaa ttccacaagg ttgaaatggt
    35821 caaatttgcc aaaccagaag aatcttacga agaattggaa aaaatgacag ccaacgctga
    35881 aaacattctt caaaaactca accttccata ccgtgtcgtt gctctctcta ctggagatat
    35941 gggcttctca gctgcgaaga cttacgactt ggaagtgtgg attccagcac aaaacaatta
    36001 ccgtgaaatc tcaagctgtt caaacacaga agatttccaa gcccgtcgtg cccaaatccg
    36061 ttaccgtgat gaagcagatg gcaaggtgaa actccttcat accttgaacg gctctggact
    36121 tgcagttgga cgtacagtgg ctgcaattct tgaaaattac caaaatgaag atggttctgt
    36181 gaccatccca gaagcacttc gtccatacat gggtggagct gaagtcatca aaccataaaa
    36241 aataaggttt agctatttct agctagacct tttttcgtaa ccaaatcaga taagcaccta
    36301 gtacaaagaa taaaatagtt aggcatataa tggtttcagc caataccagg taatccagaa
    36361 atggaagttt caaaattccc tgagccatct tgagcgaggt cgctgtgata atggttggga
    36421 aggtgagggc tgagaaggct ggttgaaaac cttgttttaa aatgttgggc agacgagtta
    36481 aaacaaagaa aaagaaggat tgagaagcca aaatcatgac aatcaagacc caagtcggca
    36541 ggctggttcc tcctactcga actagagaag ccaagagtag agagaaagga gcacagtaga
    36601 ttccttcttg tccaagcaag gctagtggga gtggatgttt ctttaaatcg ctataaataa
    36661 ggggatagag atagaaggtc aagagaaaac caaaactcaa ggtcgcatag gcaatttcga
    36721 taatacctac cagaggatag gtcaaggcag ccactgctat ccccacatag agaaccgtcc
    36781 aacttggagt ggcatgaacc ctccgccctg aacaagcaaa cttgatggta aaaccagcaa
    36841 tcaaggtcaa atccaagaga aatgaaaacc accaaatccc ttgtgctacc aaaggaagat
    36901 aagagaatac gcgaaagaca taggtcgata aaatcatccc agccatagga aaggttgcca
    36961 ttcctgacaa aagagggggc ttggtcaatt cttgcttggt ttctttccaa ttaaagagat
    37021 gcagaattag aaagtaaatc cataaaacca aaccagtcag actaaaaaga tgggatagaa
    37081 ccggcaacgt atctaaaata agatttccag ctcctgccaa acctagcaaa caacctgaaa
    37141 atactaaggg gagttttttc atcctaacct ccaataatca tgttagtttc agtataacat
    37201 aaaagcgctt aaatgaggat ttaaaaaaac gaagtccgct tatttcagac ttcattttac
    37261 tcagatatga attaggcata aggttgcaat tctggattaa ttggtgtatt agctaagttg
    37321 ttggcatagt tacagaggat tgctaggctg acaccaaaaa ccacatccaa ggcattttgt
    37381 tgagtgtagc cagcttctaa aaactcagac aaggcttcat ctcctacacg acccttggta
    37441 ttgataactg ccaaggtaaa cttagctagg gtatccaatt taggatctgt ttcaattgga
    37501 gtacgattgc gaagagcttg aatcaagtca tcattcatct ggatttgttt gatggaaaag
    37561 gctgtgtgac ctgcgacaca gaaggcacaa ccattggtca cggctgccgt gatttgcacc
    37621 acttcacgct caacgggtgt caggctgttg cgacggtgga tagatgagac aatttggtag
    37681 gcttctaaaa cagtcggggc attggccaag agaccgatta ggttgggaat atagccattg
    37741 ttgtcttttt ctactgtttc aagaatttct ttcacttctg ctggtgctga ctctactgta
    37801 tggatagtaa atgttgtcat aagatacctc ttttcttatt attgacacta atattattgg
    37861 aaaatcttat aaaatcctga ttcctaagtt tatctaagat aaagctttat tctctcataa
    37921 gattttcgtt gttatattag tttatcacac ttccaatcac ttgcataata tatattatat
    37981 atcaggctga taaaaattat ttataggcaa aaaaatcaca cgagctgtgt gattccatta
    38041 tttgtcaaaa tactttttag tttcagcaat aacgactggc gacaagacca agagggcaat
    38101 caagtttggc agagccatca aggcgttaac gatatctgcg ataatccaga ccatatccaa
    38161 ctcgataaat cctcctaaca agaccatgag cacaaaaaac acacggtaga gccagataaa
    38221 gcgagcccca aagaggaact caaaacagcg ttctccgtaa tagttccaac ctagaatcgt
    38281 tgtaaaggca aaaagtacaa ggaagatggt caagagggca ggcccaaagt gtgaaaagac
    38341 tgttgagaaa gctgactgag tcaaggcaac cccattcaag tcaccactcc aaactccagt
    38401 taccaagatg gtcaaaccag ttagagtaca aatgatgagg gtatcaataa aggttcctgt
    38461 catggaaatc aaaccttgct ctactggttc atttgtcttg gcagctgcag ctgcaatagg
    38521 agcagaaccc agaccagatt cgtttgagaa cacaccacgc gccacaccat tttgaatagc
    38581 catccgaacg ctagcaccag caaatccacc taccgcagca aggggactaa aagctgaggt
    38641 aaagactaaa gcgattgtgc cagggatttt tccgatatta aagaaaataa ctgtaagagt
    38701 tcctaagata taaatgaggg ccataaaagg aacaacagta gttgaaacct tagaaataga
    38761 cttgagtcca ccaaagactg caatcgctac aaagacagac aagacgagag ctgtgatggc
    38821 tggcgaaatc gtcgttgtat tttggataga ttctgtaatc gagttgactt gggtgaaggt
    38881 tccgattccc aagagagcaa ccaatactcc tgctactgca aacaaaacag caagtggtcg
    38941 ccacttttct cccatcccta gaaggatata gtgcatggga cctcccgcta ctgcaccatg
    39001 gtcgtccttg gtgcggtatt tgatggccaa gagtccttcc gcatacttgg tagccattcc
    39061 aaagaaagcc gccatccaca tccaaaatag agctcctggt ccaccaacct tgatagccgt
    39121 cgcaactcct atgatatttc ctgttccaac agttgatgcc aaggctgtac acagagctgc
    39181 aaaactggat acatcaccat gtcccttatc ctggataaaa ataagctgaa aggccttggg
    39241 cagacgcaaa acctgcaaga gtcctagccg catggttagg taaatccctg ttccgaccaa
    39301 taaaatcaag aggggcggtc cccaagcaaa agcatcgatt gatttaagca attctaacat
    39361 ttccttctcc tatcgtttca accccaaaag aaagagcaca tgcaagatac atgtactctg
    39421 gaatgcttag ataaatgcta aaaagcggtc tatcctagct ctgtcctttt acctgagagt
    39481 ttgagcagtt gcctgccttg ccccttcggt gcctttacgg tctctccaga gttccgtcca
    39541 tttacagtca tggaaaatca aacgattccc cacttctatt aaacttcatt cggtgttggt
    39601 atttaattga ttctaatttt acaaaaaatg ttggcttttg tcaatgtgtt tattagtaaa
    39661 aattagttca acagttttta ctttataaag tccagaatac tgctatcctt taaaagtgac
    39721 aatagtcgca ccactgcctc cagcattttg tggggcatag ccgaaactct tgacatgttt
    39781 gtttctttgc aagtatttgg taactccttc acggatgact cctgttccga taccatggat
    39841 gatatcaact tgagccatat tgttaagcaa ggcttggtcg atgaaggtat ctagctcatt
    39901 catggcttct tcatagcgct tgcctcgaag atccagtcta gcttgaggtc ctcgcccaga
    39961 agttcgtttc acaacattga cctgtttctt cttgactggt ttttcttgct gggcttgaac
    40021 aagatcaaac tctttctctt ccaaggtcat cttaatcaag ccaacttggg cttcccagcg
    40081 accgtccttg agttgactgg tcaaggtacc acgctgacca taactgagaa ccacgatatc
    40141 atctcccacc tttggagctc gttttttctt ggccttttga aggaccttat ttttagacaa
    40201 gtccactttt tcaggagcca attttttcaa cttggccttg gcttcaatga tttcgtgggg
    40261 cttgagttgg gatttactgt ggagattttt gagaatctgg tcactttcac ttagggccat
    40321 atccacaatc tcagcagcct gttcacgcgc cttgttaagc tcggtttcct tttcacgatt
    40381 aagctcgttg tagagttttt ttagcgcacg gttcatcttg agattttctt gctccacctc
    40441 acggatattg tccaaacgtt tgcggctttc cagcgtctgc tcttctaatt gctcaatgat
    40501 acgattgacg tcattgtcct gatcgatctg ctgactggca tctcctacga taacttcaga
    40561 taggcctaga cgtttggcaa tttcaaaggc attacttcgg ccaggaacac cctgcataaa
    40621 gcgataggtc gggcgaagag ttgcagtatc aaactccata ctggcatttt gcacaaaggc
    40681 tgtctcaata ccgtaggcct tgagttctgg atagtgggtc gtcgccatgg tcttgatttg
    40741 acgcaggcga aggtcctcca gaatagccat ggcaagggct gctccctctt ggggatcagt
    40801 accagccccc aactcatcca aaagtaagag tgaatgttgg ttgaccttgc caagaatatc
    40861 cacgatattg gtcatatggc tagagaaggt agacaagctc tgctcaatag actgctcatc
    40921 tccaatatca gcaaagattt cttcaaaaat accaacacga cttcccttgt ctgctaaaat
    40981 cggcaatcct gactgggcca tgacctgtgt caagcccaga gttttgagca tgatggtctt
    41041 cccacctgta ttgggacctg taatgacaat agctgttaaa tcttgaccaa aatggacatc
    41101 atttgcgacg gcatttttga ccaaaggatg gcagacatgg agcagttgaa tctcttgatt
    41161 ttctgacagc tgaggcacga ctgcttgtct ttcttggata aatcgaacct tggcacgaat
    41221 caagtccaga tgaccgataa tccaagcgtc attagcaatc tcagccgcat gagggcggac
    41281 acgctcagaa atttcttgga gaatgcgaag catttcatag cgctcatctg ctcgcagact
    41341 agcaatttct tcgctcagtt tgactacctc acgtggttcg atatagacgg tgtttccact
    41401 agcagaaata tcatgaacga cacctgcaat cttattgcgg taggtgtttt tgactggtaa
    41461 aacctgacgg ccatttctgc tagcaacaat tccttccgtc aacatctgcg ctttttgctt
    41521 gagcaagtct tgtaaaacat cgcgtacctg actctcgcta tcatgtattt ttcgacggat
    41581 tcgcgccaat tcttcactgg caaaattttc aatgaaaccc gcatcattaa aggcctgaag
    41641 atttccttgt aattgcggaa aatcatgtaa tttctcaaac caaagggcta attcttccaa
    41701 gctgacattt tccagattgg cataaaaact ttgaagttct cggctggcaa gaagcacgcg
    41761 tttcaagagt aggaactcct cgatattgag atccgctccc atctccaacc tcttgcagac
    41821 tcctgcaatt tccttagttg agagaatagt aaaatgcggt tgctcgacga aaagagcctg
    41881 catttctttc atctcagcaa aagcctgttt gattttatct gctttggcag tcggagccag
    41941 ctgtctcaat tgctccaagc cctgctcggt caacaaatga ggctcaaaca aggccttgac
    42001 cttatcgaac tctaatgttt ctaatatttt cttattcatc tttatttcct tgtctttgaa
    42061 tgtctaggtg tggtagcttt ttacattctg atagattcga gtcaaatctg taaacaaagg
    42121 gctaggaaaa ctcctgccct ttttatccga ttaaatttgt cacccagatt tgtttgagcc
    42181 aactggttgt tatcggtatg ctctggatga tgtgttttgc gacgatactc ttttcaagag
    42241 gattttgtat aactgccatg gggatggtcg ccaagattgt caaggccatt tgcaagacaa
    42301 ataaggtcac caacatggac aagatacctg ctgaaacttg gaacaactta ccacccagtt
    42361 ttttgctagg aatcaagtgt aagagaagac caagcaaacg accaatgcta tagaaaatcc
    42421 caaatacaag caagtagccg atacctgcat aaaagacctt atccagctga aagagttgat
    42481 ccgatgggaa aaagaaagtc ccctgacctt cctgcgaatt tgcataaggg agcaataaat
    42541 ggaattgctc tccaagcccc ttataaaact ggccagccat aaaagccgat gccatggctg
    42601 aaatcaggta ataaacctgt aagagcaggc ctctccgata gccgatataa aatccccaaa
    42661 ccaagaccaa tagaagaagg aatgaaatca taaggaatcc tcaatcttgc tctgttcttg
    42721 cttacaagtc acaagcttgt gacggagttc ttctagctct tgctccttat cgtcaaattc
    42781 aatctcacgg ctgagctgag ttgataaaca gttgactgcc aacaaaagag cgattgtttc
    42841 atcatctgcg ctaggcattt gttctttaat tgcttggtat ttttctgtcg caaccttagc
    42901 gatttcctcc ataaaaaggt tatcatgttc gcttgtcaag gttaacgatt ttttcccgaa
    42961 tgtaaatttg aatcgattta gatttgccat aaaattcacc tcacgatatt ataccaaaat
    43021 tcgctaattt tgtcagtttt tacaaatttt actgcttttg tggtacaata gaaactatgg
    43081 caagtatcac actcacacca agcgaaaagg atattcaggc ttttcttgaa cactatcaaa
    43141 ccagtctggc tcccagtaag aatccctata tccgctactt tttgaaacta cctcaagcaa
    43201 cggtttctat ctatacttct ggaaaaatct tgcttcaggg tgaaggggct gagaaatacg
    43261 ctagtttctt tggctatcaa cttgtagagg aaaacagggg gcaaaatctt cctttaattg
    43321 ggacagatga ggtgggaaat ggttcctact ttggtgggct tgcagttgtg gctgcctttg
    43381 tcacatctga ccagcacgac tttttacgaa aactcggtgt gggggattct aagactctga
    43441 ccgaccaaaa gatccgtcag attgctccta ttctcaagga aaaaatccag caccaggcac
    43501 tccttctctc acccagcaag tacaacgagg tcatcggaga ccgctacaat gctgtttcgg
    43561 ttaaggttgc cctccataat caggctatct atctcctcct tcaaaaaggt gttcagcctg
    43621 agaaaattgt gattgatgcc tttaccagtg ctaaaaatta tgacaagtac ttggcacaag
    43681 aggccaatcg tttcagcaat cctatcagct tagaagaaaa ggctgagggc aaatacttgt
    43741 ctgtcgcagt ttcttctgtc attgcgcgtg atctctttct ggaaaatctt gaaaatttgg
    43801 gacgagaact gggttatcag ctcccaagtg gagctggaac ggcttctgac aaggtggcta
    43861 gccagatttt gcaagcctat ggtatgcagg gactcagctt ctgcgccaaa ttgcacttta
    43921 aaaacactga aaaagcgaaa aaacgcttag aaaggtaagt tatgaattca tttaaaaatt
    43981 tcttaaaaga gtggggattg ttcctcctaa ttctgtcatt actagcttta agtcgtatct
    44041 ttttttggag caatgttcgc gtagaaggac attccatgga tccgacccta gcggatggcg
    44101 aaattctctt cgttgtaaaa caccttccta ttgaccgttt tgatatcgtg gtggcccatg
    44161 aggaagatgg gaataaggac atcgtcaagc gcgtgattgg aatgcctggc gacaccattc
    44221 gttacgaaaa tgataaactc tacatcaatg acaaagaaac ggacgagcct tatctagcag
    44281 actatatcaa acgcttcaag gatgacaaac tccaaagcac ttactcaggc aagggctttg
    44341 aaggaaataa aggaactttc tttagaagta tcgctcaaaa agctcaagcc ttcacagttg
    44401 atgtcaacta caacaccaac tttagcttta ctgttccaga aggagaatac cttctcctcg
    44461 gagatgaccg cttggtttcg agcgacagcc gccacgtagg taccttcaaa gcaaaagata
    44521 tcacagggga agctaaattc cgcttatggc caatcacccg tatcggaaca ttttaagaaa
    44581 cctaagaggc cgagaatcac caatctcagc ctcttcttct atcgtgagaa aatgattggt
    44641 aactatctaa acttaccaga acagaaacac ctcaactctc acctattcat gcaaaggaat
    44701 tttatggaag tttatttttc aggaaccatt gaacggatta tttttgaaaa tcccagcaat
    44761 ttttatcgca tcctcctcct agaaatcgac gatacggacg cagaggattt tgatgatttt
    44821 gaaatcattg tcacaggaac catggctgat gtaattgagg gcgaagacta tactttttgg
    44881 gggcaaattg tccagcactc caagtatgga gaacaactgc aaatcagtcg ttatgatcgc
    44941 gcaaaaccaa ctagtaaggg cttggtcaag tacttttcaa gtagccattt caaggggatt
    45001 ggtctcaaga cagctcagaa aatcgtggat acctatggcg aaaataccat tgacgaaatt
    45061 ttgcaacacc cagaaaagtt agaaggcatc gcaggactct ctgccaaaaa tcgcgaggct
    45121 ttcgtctcca ctctccgtct caactacgga acggagatgg ttttggccaa actagccaac
    45181 tacggcattc ccaacaaact agcctttcag attcaagact tttacaagga agaaaccctt
    45241 gatgtggttg aaaattatcc ctaccagctg gttgaggata tcaagggttt gggctttacc
    45301 attgctgacc aactagctga ggaactaggc atcgaaagtc aggctcctga acgcttccgc
    45361 gctggtctag ttcacagtct ttttcaggcc tgtatggaaa caggggacac ctatgttgaa
    45421 gcacgggatt tgctggaaca aacccttact ctccttgagt cttcccgtcc cgtggaacta
    45481 gaccccagcc aagttgccca agagctctcc tacctgatcg aagaagacaa agttcagcag
    45541 attgatacca agatctttga caacagcctc tttttcgctg aggaaggcat ccgcagtcac
    45601 ttggttcgta tccttgaaaa aggaaaacag aagagtcatg atttaaaaac cattcaaaaa
    45661 catatcacta ctatcgagaa agagcttgga attgagtatg ataacattca aaaacaggct
    45721 atctgtgatg ctatccagaa taaggtcttt atcctgacag gtggacctgg tactggtaag
    45781 acaactgtta tcaatgggat catcgctgtt tacgccctgt tagagggact tgacctcagg
    45841 aagaaaagca acctgcccat tcttcttgct gctccaactg gtcgagctgc tcgtcgcatg
    45901 aatgaattga caggtttgcc tagcgcgacc atacaccgcc acttgggaat gacaggtgac
    45961 gatgatacca gtcatctgga agattaccta gatgctgact ttatcatcgt ggatgaattc
    46021 tctatggtgg atacttggct ggccaatcaa ctcttctcca gtatctcttc taacagtaag
    46081 atcctcatcg taggtgacag tgatcagcta ccttctgtca gtcctggaca ggttctagcg
    46141 gatctgcttc atattccttt gattcctcag actcgcttgg aaaaaattta tcgacaaagc
    46201 gaagaatcaa ccatcgtcac cctagctagt cagattcgac agggcatctt gccagctgat
    46261 ttcacccaaa aaaaagctga ccgttcctac tttgaaattg ctagtggcca tattcctgcc
    46321 accattgaaa aaatcttagg tgccgccctc agaaatggta ttcctgcccg tgatatccaa
    46381 gttctggctc ctatgtaccg agggacggca gggattgatg ctatcaatca gctcatgcaa
    46441 gacctgctca atcccccaca aaaagatcaa ctcagttttg aagctcctca gtgccactat
    46501 cgtaagggag acaaggtcat tcatttggtt aacgatgctg aaatcaatgt ctttaatggg
    46561 gatttaggag ctatcacaga cctgattcct ggtaaataca ccgaatcgaa acaagacgag
    46621 attgtcattg attttgatgg taatgaagtc tcttaccccc gtaacgaatg gtacaagatt
    46681 cgcttggcct atgccatgag tattcataag tctcagggaa gtgagttccc agttgtcatc
    46741 ctaccgatta ctagtgctag ccgacgtatg ctggagcgaa atctcatcta cacagccatt
    46801 acacgtgcca aaagcaaact catcttacta ggcgaattac aagccttcga ctatgctacc
    46861 caacacatcg gaactgcccg aaaaacctat ctgattgaac gcttcagtga tctactggag
    46921 aatgttgaag aaaagcaaca agctgtctct gaaacagtca catcaagtgc ctctgaacaa
    46981 tcctacatcc taaccgaaga aaactgggac agcattccag ccatgattgg gattacagac
    47041 acagacctca aagagatttt tggaaaatag tgcatcagaa aaaccaccaa ttcaggtggg
    47101 ttttctgttt attaagatta ttttactgtt gctgtgcttg taatcaattc aacagctttt
    47161 ttgatagtga tatcatgttt caacatgtca gctgaaagca agttttgaac ttgtgcaact
    47221 tccatgttgt agtctgctgc caattgctca acttcttttt ggatttcttc ttctgaagca
    47281 tcaaatcctt cagctttggc aactgcttcg ataacaaggt tagtcttagt acgtgactca
    47341 gcttctgctt ggtattggtt gtgaaggtct tcttgagtag ttccagtgat ttggaagtac
    47401 atgtcagggt tgatcccttg acgttgcaag ttcccaagga attcatttac tgaacggtga
    47461 acttcttcgt ggatcatttc ttctggaagt tctacgattt cagcattttc tacagctgta
    47521 tcaattgctg caccttcaac tgcatctttg taagcttctt ctttagcagt agccaattct
    47581 ttgcggtatt tttctttcaa gtcagcaagt gtttcaactt cttcatcaat gtcttttgca
    47641 agttcatcgt caagagctgg aacttcttta gcttttactt cgtggatagt tgtcacgaat
    47701 ttagcttctt tacctgcaag gtcttctgct tggtagtctt ctgggaatgt tacgataaca
    47761 tcaacggttt cgccagctga gtgacctacc aattggtctt cgaaaccagg gatgaattga
    47821 cctgaaccaa gtccaagtga gaagttttca ccttttccac cgtcaaattc aacaccgtcg
    47881 atagaaccaa cgaagtcgat cacaacagtg tcgccgtttt cagcagcagc ttccttgata
    47941 accaattcag ccagattgtt gcgttcgcgt tcgatacgct cttcaacatc agcgtcagtt
    48001 acttcttttt ctacatcaac tgatacttca aggtttttgt agtcacccaa ttttacttca
    48061 ggttttgtaa cgacttcagc agtgataacc cagtcttgac ctttttccat tgaagttacg
    48121 tcaatttttg gttgggcaac cacttcaaga ccagcttctt ttacagctgc ttcataagcg
    48181 tttggcaaaa gagcgttcat aacgtcttgg taaagtgatt cttcaccaaa ttttttatcg
    48241 aagataggac gtggaaggtg acctttacgg aaacctggaa cattaagaga tttcttcact
    48301 gacttgaaga cacggtccaa ttctggtttg atttggtctt gagagatagt gaaagtcaag
    48361 acaccacggt ttgtttcttt gttttcaaat gatacagaca ttctgtcatt tctccttaaa
    48421 attttttaaa tacagtctat tataacattt tctataaata aaatctagac taatgcagcc
    48481 aattatagac aacaagcctt tttcctaact aaaacactca taaagtatag acaattttag
    48541 actactttgg atatcttttg gttagtcatt ttccaaaact aatttatcta atttttcagt
    48601 tatatagtag attgaaacta gaatagtaca cctctacttc taaaacattt ttagaaatcg
    48661 atttgactgt cctgatcgat ttgtcctgtt cttatttcat tttactatat agatcgcatt
    48721 cgaaagaatt cgttccaaaa ttgcttggtt cgttcttctc ttggacgact aaagaattga
    48781 tgggcattat ttttttcaac aattttccca ctgtccataa aaatagcatg atgggctaca
    48841 tctctagcaa aacctatctc ataaagctga tcaaaaactc aatctgagga gaaaatccaa
    48901 taaggctttc cataataaag gacatcctat caagattttt gaacacccga taggctgcct
    48961 ttttccgatt ttgaagactt tttgtccagc ttcttttacc ttataaataa caacaaaaat
    49021 caaagacaag ctagaaagat taccgcagat aactcaaaca gtgttttgag gttgcagata
    49081 agactgacgg agtcagtaac atatgcctaa gacaaagcga tgctaacctg atttgaagag
    49141 attttcgaaa aatattaaat atactctaca atttgatcaa tcaattcttg gtcgtctaaa
    49201 cctgtaactt gtataacaac atctttgact gattgtttag ccaacaattc acctaatcga
    49261 atactttctt catcatttac atcgcgatag tcaaaggcat agccaactgt tttaagtagg
    49321 tttttatatg acaaactgag ttctttcaat tcacgtatcg gccggatgaa tcgttcatta
    49381 tagcctaatt ttcggattgg agtacgagct acgcgactaa cctcgtccac tatgaaagga
    49441 ttttcaaacc gttctataat gactttgtga taattctcca attctttttt atcaaagttc
    49501 catttggcaa tcaagagact ccgaatttca gctaatacag attcaatccg agatttgata
    49561 ttaggatttt gaagagcttc caaaattgtc ttggcaccat aatgcgcacc aatgtaagct
    49621 gaagttgcat gtccagaatt gactgaaaaa agttttcgct caataaaggg ttctaaatct
    49681 tcttcataat gcacatcttt tagacgtaaa tctggatttt taagacgctt ggtttccacg
    49741 acccattcat taaagggctc gaccacaaca aaaagggaat cttcgtgact ttgtgctgga
    49801 acaatcctgt ctactgcagc atttggaaaa cctatgtagt tatcagcaaa tgtcaaacct
    49861 tccggactta aatatttctt gacttcttga taaagaaatt gagacccgcc aatcatattt
    49921 tcacaggcca taacatccaa tacctgtgta ttccctgcaa ctcggcgagc ttcgattcct
    49981 ttggctagaa gttcagcgat aaaagggagt atattaggtc cgattgcagt agtaataata
    50041 tccgtctttt gaatcgcttc aatgacttgc tcaggatgtt ctttgctatt aatgccagcc
    50101 acattagtta cttctatacg agactgtcct ttctgtgcaa tttcaatttc atacttgccc
    50161 ttttcattta gagcatgaat tatctgatta ttgacatcca caaaatcaat atggaaacca
    50221 tttttaaata gaatttcacc tataaaacca cgaccgatat taccggcacc aaaatgaaca
    50281 gaatgtttca ttcaatttct ccttttccta tttgagggac atatatacta gatggcaaat
    50341 gacgaaattc aattattcaa cagcatcaaa taagcgcaat acttcctctt ttgactgagc
    50401 atcggctagt ttaagaacat tatctacatc tgcacagaag atagaaattt tctgaataat
    50461 ttctaagtgt tcattaccaa taccagcaat accaaaaaga accgttgcca cttgagggtt
    50521 agatacattc ccaaaatcaa cccctctagg gacttgaacg actgtaatac cagacttgag
    50581 gacatccttt tttgctgcat ctgttccatg cggtatggcg ataaagttac ccatgtaaac
    50641 agatagctct ttatctcgct caatcatggc ttcaatatag tcttcattaa catatccacc
    50701 ctcataaaga acttgcccac aataacaaat agcttcctcc ttgttagaaa attgtttatt
    50761 aagcttaatc aaatgttttt caagtttcat attagttctc caatttctta attttttcgt
    50821 taaaaatagt atttagcatc tgataaataa tggattgatt tcccgtcttg taaatctctg
    50881 tataaagatg attttcaata atcgactgac taatagctgt cattaaatct ctcgcttcct
    50941 cgctagcttc tttagacatt agcattagca gacacctttt gacctcttct tcctctcgtt
    51001 tcattgacaa tgcggagata ggtagtttga gttcaaacat agtaaaacta tttgttgtta
    51061 ctttgctaga ctgactatgt aaaagcacca gaccagtatt aggaatagcc ataggactat
    51121 tctggaagtg agataacaat tttcttgtta ggtaatctct gtcactcaca ttcttctgat
    51181 attggatgat ttgttctacc gtgtgctcaa atgattgatt attctccaat tgaaccaact
    51241 cgaattgact caaaagctga ctactagata ttaaataatc ttgcaagtca taacttttct
    51301 ctggagcgat agcatcacgc gcgacaattg tacgatgtgc ctgtacatac tgaagtcgtt
    51361 cctgtaattc aagcaattct ttgatggttg gaaaactaga aattacatcg atttcctgat
    51421 tagtcagcgg cttggtagat aaaatataat catactgact gagatcaata ctgtggtaat
    51481 ctactagaga ctgaatatct atcaactcta caaaaggagc aacactctta attttactga
    51541 ctaataaatc tctagtgacc cgacgttcat ccgttaaaag caaaacacgg actggataaa
    51601 tctctggact acggcctaag ctagagataa aatgtagggc aatcatgcca tactcatact
    51661 ctgtatgaag atatttcgga aaaatatcat tcactaaaag actgatgact gtataaagaa
    51721 atttattcct ttcaactaaa atctggacag attctggcaa attttcaccc tgaaaaagga
    51781 tagggacgcc taaactgagc cgaatatgat ggaaaagaaa attgaataaa accttgtcct
    51841 taaaaaagtc aatcttggta tacatggaaa ccgtatcaat cagatttgaa atattgtaga
    51901 aaaattcacc atcaaatttc tccgtaaaga gcggattgtc ctgacgttta atgattaatt
    51961 catccaagat gctcgcaaaa tagataattt cctgaatact ataaaattgt gcagtctgct
    52021 tagagtaagt ttgaaaaatt ttttgagaaa tttccaaagc ctgcttacta gtattaggtg
    52081 aattttcaat gttttgctcc tgacctataa gagataacaa gatcgcaaaa aacatcttca
    52141 tcctagcatc catatctgga aaacctgaca gttgcttatt aacaatctga ctggccagcc
    52201 tagttctatc tgcttctaaa atatcaaagc tcccaaaatt accggttgaa aaatctgcta
    52261 ctgagataca gtttgtcagt aaaatagcca aaaatcttct cttaccaact gaatcaccag
    52321 aaatccgata acctttttgt cgttcaattt tcagatcaaa gtctaaaaga cgcttatcaa
    52381 tatctgaaat atcctgaata atagttacat tactgacttt cgtgcactct tgcaatgctt
    52441 cattggtgac aaaaccgctc tcagtcagaa ggcgataggt aatcaactct tgtctttctt
    52501 ggggactata ctcaactaac acttcaagtt ctgtcggcaa atcatccaac tctccagtca
    52561 aaatatagta tttcccatgc ttcgttattt cgattccata ttgctccatg ctatctgtca
    52621 aatctgataa agttcgataa attgttctag atgaaacctg taagatttca gtcatatctt
    52681 gcattgaaag cttccctaca tgtaggaaag ccttcaataa ttgttcttct cgtttggtta
    52741 atagcattcg taatttagaa acctctagtt ctatttgtac attctagcag ccattttgtc
    52801 atattctggt gttgttacta aactatccac aataagaaat tgagaattcg gtgctgcttg
    52861 ctgcacttct gcctgtaaag aaatagttgt tacaatcatt atgtttttag aattaaattc
    52921 acctaattct gaaattttgg cagtagaaac tggaatacga atattcttgt ttctaaagat
    52981 agcccgaatc gtttcacagc ccatagttgc agttccctgt gctttaccat aagcaactac
    53041 tacagcatca atatggttaa ggtcaatttc ctgactatct actggtgctg aagttggtat
    53101 atctccttca atttctgcta ttggaaaagc tcctgttaat gaagctacaa tttcatcata
    53161 acgaggggac gctaagaaat tatcaacaga aacatgaata gcacttggac tcttgtcttt
    53221 agctcttggt gtcagttctt cctgagtaac aattaatgtt tttggtgtat caagcaaatt
    53281 tgagattgcc tgattagata ctggaatctc tagacctgct tttttaacct tatctcgaag
    53341 aatactagct cccatagcag agcttcccat accagcatcg caggcgaaaa tgattttttc
    53401 cactgagtct gtcgaaacaa ctgcatcaac agaagttgat actaactgac ctttagactg
    53461 agccttagct gcttgggtag ccgcctgagc agcttcgagc gaatcctcag ttgacttgtc
    53521 tgcatgaaga ataagggctg ctacaaggaa agaaacaact gctgccacta aaacacctaa
    53581 aagaacattt agatgaggcc aaacaccttt tggcgccgta gccataatcg caataataga
    53641 acctggtgaa gctggagatt taagaccagc gtctaagagt tgaaaagtaa aagttccaga
    53701 gatacctcct gccatagcag ctaaaaatag agtaggcttc atcataacat aaggaaagta
    53761 aatttcatga atccctccga agaaatgaat aaccattgcc ccccaagaag aagatttagc
    53821 agaaccttta ccgaatacag cataagctaa tagaattccc aggcctggtc caggattagc
    53881 ttccaatagg aagagaattg acttaccagc ttgagctacc tgttctactc ccagaggagt
    53941 aaaaatgcca tgattgaggg cattattgag gaaaaggact ttagccggtt cgatgataat
    54001 attagccata ggaaggaggc gagcattgac aatagcctca acaccattcc caacagctcc
    54061 agtaagagtc gatacgactg gaccgattgc gtagaaagcc aaaagcaata atgcaaaacc
    54121 aacgagacca gctgagaagt tattaactaa catttcaaat ccgggacgaa ttttttcctg
    54181 gaacttctca tcaaatttct tgatagtcca tcctcccagt gggcccatta ccatagctcc
    54241 gataaacata ggaacactag aacctgtgat tgcaccaaca gtagcaatag ctcctacaac
    54301 ggcaccacgt tggccatgga tcatatatcc acctgtgtaa ccaatcagga ttggcaataa
    54361 atacgttaac ataggaccaa caacagtagc taactgttca tttggcagat agccatcagc
    54421 gataaagagg gcagtcaata ctccccaagc aataaatgct ccaatattgg gcataaccat
    54481 atttgaaagc gatgtcccta gtttttgaac cctgactttc aatgatactt tttcttccat
    54541 tagatcatcc tccttttctt aaaactagta tatacaaagt taaaaacctt taaaactaaa
    54601 gttttgtcac agattttgtg tcaaaacatt acttttgacg aggctgggca aaaaggctta
    54661 aaaattataa aacgcatagt ataggatgtt gaaatgatga actgcacccc aaaagttaga
    54721 cagaaaaaat ctaacttttg gggtgttttt atcatgaagt taacttatga tgataaagtt
    54781 cagatctatg aacttagaaa acaaggatat agcttagaga agttttcaaa taaatttggg
    54841 ataaa
//
LOCUS       DAVBCN010000005        48562 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVBCN010000005 DAVBCN010000000
VERSION     DAVBCN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48562
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.005"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(72..1208)
                     /gene="lctO"
                     /locus_tag="VQ497_000273"
     CDS             complement(72..1208)
                     /gene="lctO"
                     /locus_tag="VQ497_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HET2516642.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(1833..3323)
                     /gene="lysS"
                     /locus_tag="VQ497_000274"
     CDS             complement(1833..3323)
                     /gene="lysS"
                     /locus_tag="VQ497_000274"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET2516643.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLITKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKTIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(3451..3597)
                     /locus_tag="VQ497_000275"
     CDS             complement(3451..3597)
                     /locus_tag="VQ497_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HET2516644.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAGDTF
                     TSFQ"
     gene            3823..4491
                     /locus_tag="VQ497_000276"
     CDS             3823..4491
                     /locus_tag="VQ497_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2516645.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRILV
                     LAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFYYVVLPQATAVALPSFSANVIFLIKETSVFSAV
                     ALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPST
                     LSRK"
     gene            4451..5128
                     /locus_tag="VQ497_000277"
     CDS             4451..5128
                     /locus_tag="VQ497_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2516646.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIEISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLDFTRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GAAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            5129..5886
                     /locus_tag="VQ497_000278"
                     /pseudo
     CDS             5129..5886
                     /locus_tag="VQ497_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            5898..6759
                     /locus_tag="VQ497_000279"
                     /pseudo
     CDS             5898..6759
                     /locus_tag="VQ497_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(6839..7486)
                     /locus_tag="VQ497_000280"
     CDS             complement(6839..7486)
                     /locus_tag="VQ497_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2516647.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(7483..8181)
                     /locus_tag="VQ497_000281"
     CDS             complement(7483..8181)
                     /locus_tag="VQ497_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516648.1"
                     /translation="MGKLSSHMWRLNKIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDVGGDLFLQLLFLVFLFQ
                     SIFYFTRQKRRFIE"
     gene            complement(8191..8943)
                     /locus_tag="VQ497_000282"
     CDS             complement(8191..8943)
                     /locus_tag="VQ497_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516649.1"
                     /translation="MKQFVQFYKKDFLSVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLNNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            complement(8943..9707)
                     /locus_tag="VQ497_000283"
     CDS             complement(8943..9707)
                     /locus_tag="VQ497_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516650.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(10386..11018)
                     /locus_tag="VQ497_000284"
     CDS             complement(10386..11018)
                     /locus_tag="VQ497_000284"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET2516651.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYRELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(11052..11753)
                     /gene="pyrF"
                     /locus_tag="VQ497_000285"
     CDS             complement(11052..11753)
                     /gene="pyrF"
                     /locus_tag="VQ497_000285"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET2516652.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQNWN"
     gene            12074..12166
                     /locus_tag="VQ497_000286"
                     /pseudo
     CDS             12074..12166
                     /locus_tag="VQ497_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(12488..13201)
                     /locus_tag="VQ497_000287"
     CDS             complement(12488..13201)
                     /locus_tag="VQ497_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2516653.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQG
                     KVNDQQALVLLMMCLPFSLALAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEGFSWNKTLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(13194..13889)
                     /locus_tag="VQ497_000288"
     CDS             complement(13194..13889)
                     /locus_tag="VQ497_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2516654.1"
                     /translation="MTGQFLADKGQSIILGQKSQNLTSGELKRIGLVSDTSGFYEKMS
                     LYNNLLFYSKFYNISKSRVDNLLKRVGLYDSRKMVAGKLSTGMRQRMLLARALINKPA
                     VLFLDEPTSGLDPTTSRTIHELILELKTAGTTIFLTTHDMNEATLLCDYVALLNKGKL
                     VEQGAPSELIQRYNKDKKIKVTDYNGNQITFDFTSLEQVSQADLENIFSIHSCEPTLE
                     DIFITLTGGKLNA"
     gene            complement(14229..15561)
                     /locus_tag="VQ497_000289"
                     /pseudo
     CDS             complement(14229..15561)
                     /locus_tag="VQ497_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812020.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
     gene            complement(15545..16343)
                     /locus_tag="VQ497_000290"
                     /pseudo
     CDS             complement(15545..16343)
                     /locus_tag="VQ497_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824573.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50 family protein"
     gene            complement(17133..18332)
                     /gene="divIB"
                     /locus_tag="VQ497_000291"
     CDS             complement(17133..18332)
                     /gene="divIB"
                     /locus_tag="VQ497_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HET2516655.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLSKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
     gene            complement(18342..19400)
                     /locus_tag="VQ497_000292"
     CDS             complement(18342..19400)
                     /locus_tag="VQ497_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HET2516656.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVCWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VAKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQLENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(19402..20754)
                     /locus_tag="VQ497_000293"
     CDS             complement(19402..20754)
                     /locus_tag="VQ497_000293"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET2516657.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(20858..21499)
                     /locus_tag="VQ497_000294"
     CDS             complement(20858..21499)
                     /locus_tag="VQ497_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2516658.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTFL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNCVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTTSLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(21501..23522)
                     /locus_tag="VQ497_000295"
     CDS             complement(21501..23522)
                     /locus_tag="VQ497_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2516659.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLSYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLMSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            complement(23593..23892)
                     /locus_tag="VQ497_000296"
     CDS             complement(23593..23892)
                     /locus_tag="VQ497_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET2516660.1"
                     /translation="MKTKKHRLLALALISSFTLLGAASAAVQYPDGGVWTYGEGSGGG
                     WAFSNYYHGKKYHYSSLVSRWNSHSDKGEASAGKTSYAWIWTKWGEQVAFYCDYD"
     gene            complement(24258..24512)
                     /locus_tag="VQ497_000297"
     CDS             complement(24258..24512)
                     /locus_tag="VQ497_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HET2516661.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            complement(24532..26373)
                     /gene="typA"
                     /locus_tag="VQ497_000298"
     CDS             complement(24532..26373)
                     /gene="typA"
                     /locus_tag="VQ497_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET2516662.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            26535..27260
                     /locus_tag="VQ497_000299"
     CDS             26535..27260
                     /locus_tag="VQ497_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HET2516663.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVQVNELLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
     gene            complement(27300..27527)
                     /locus_tag="VQ497_000300"
     CDS             complement(27300..27527)
                     /locus_tag="VQ497_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516664.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(27616..27996)
                     /locus_tag="VQ497_000301"
     CDS             complement(27616..27996)
                     /locus_tag="VQ497_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2516665.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            complement(27996..28265)
                     /locus_tag="VQ497_000302"
     CDS             complement(27996..28265)
                     /locus_tag="VQ497_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HET2516666.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            28329..29237
                     /locus_tag="VQ497_000303"
     CDS             28329..29237
                     /locus_tag="VQ497_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET2516667.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            complement(29291..30046)
                     /locus_tag="VQ497_000304"
     CDS             complement(29291..30046)
                     /locus_tag="VQ497_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET2516668.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(30067..30996)
                     /locus_tag="VQ497_000305"
     CDS             complement(30067..30996)
                     /locus_tag="VQ497_000305"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HET2516669.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            complement(31011..31634)
                     /locus_tag="VQ497_000306"
     CDS             complement(31011..31634)
                     /locus_tag="VQ497_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HET2516670.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFSHQLVFEESGKIYMKPVKDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            complement(31627..32865)
                     /gene="hflX"
                     /locus_tag="VQ497_000307"
     CDS             complement(31627..32865)
                     /gene="hflX"
                     /locus_tag="VQ497_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET2516671.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            complement(32858..33742)
                     /gene="miaA"
                     /locus_tag="VQ497_000308"
     CDS             complement(32858..33742)
                     /gene="miaA"
                     /locus_tag="VQ497_000308"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET2516672.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKCFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            33863..34033
                     /locus_tag="VQ497_000309"
     CDS             33863..34033
                     /locus_tag="VQ497_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HET2516673.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            complement(34081..34920)
                     /locus_tag="VQ497_000310"
     CDS             complement(34081..34920)
                     /locus_tag="VQ497_000310"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET2516674.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPIITLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(35017..35976)
                     /locus_tag="VQ497_000311"
     CDS             complement(35017..35976)
                     /locus_tag="VQ497_000311"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HET2516675.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENTFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            complement(36103..37101)
                     /locus_tag="VQ497_000312"
     CDS             complement(36103..37101)
                     /locus_tag="VQ497_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2516676.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DLHEGEPGYSAKLDRDHDGVACELKNAPKGTFKAKQAATTQTDTTSSTASGWVKQDGS
                     WYYFDGNGNLVKNAWQGNYYLKADGKMAQSEWIYDSSYQAWYYLKSDGSYAKNAWQGA
                     YYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDA
                     TYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTA
                     SSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDG"
     gene            complement(37151..37768)
                     /locus_tag="VQ497_000313"
     CDS             complement(37151..37768)
                     /locus_tag="VQ497_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2516677.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQNIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            complement(37769..39490)
                     /gene="pabB"
                     /locus_tag="VQ497_000314"
     CDS             complement(37769..39490)
                     /gene="pabB"
                     /locus_tag="VQ497_000314"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HET2516678.1"
                     /translation="MHRKTVIDFRTLGERYTFAQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQYLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            complement(39559..45246)
                     /locus_tag="VQ497_000315"
     CDS             complement(39559..45246)
                     /locus_tag="VQ497_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2516679.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEETPKEEAPRTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKSEEKVAVKPESQPSDKPAEES
                     KVEPPVEQAKVPEQPVQPTQAEQPSTPKESSQEDNPKEDRGAEETPKQEESTPDTKAE
                     ETVEPKEETKTAKGTQEEDYVGEALVPEQLPEYKVTEGTLVEESTTELDYKTETTEDP
                     TKYTDEETVLRNGEKGSQVTKTTYKTIGGVKTDQVLSTSTEVTKEPVNQQVIRGTKPI
                     EGTTVETREEIIPFETKEQEDDTLKRGTRQVTQEGVNDKKQITETYKTIRGEKTSDAP
                     TITETVIEKPQDKIIKNGTKELEKPTLEWAKTDKDVLKKSATASYTLTKPAGVEIKSI
                     KVALKDNTGTVVKEVTVEENNLQATLDNLKYYQGYTLSTTMVYDRGNGEETEILEDKE
                     VQLDLKKVEIKDIKETRLMKVDENGNETDSSLLETVPENLTSYYLKITTNHNKVTRLA
                     ITNIEEVTEEETSLFKVTASAPDLIQRDSNNSLSQTYTYYIEKPKASQNNVYYNFKDL
                     VDAMQKNPNGEFKLGSDLNATNVPTPSKSYVTGKFTGHLTSVDGKHFSIHNTARPLFD
                     RVEGGSIKNINLINVNINMPWANQLASLAQTVKNATVENIKVTGTIVGNNNVAGILDK
                     LDDAGIMTNVAFIGKVQSVGDKGWNLAGIASEIYRGNLNKAYADVELKGLNTRAGAIV
                     GHLAHDFNPRDVGAWSNVRNSVAKGTIDVARGDVGGLIGKDWPWGTIDNNISLVNVKR
                     GDIFFGSNDMDDEFVSNWIKPNNATVIGVSSGDKTHKQSSKIKEISQVEADKKIKDMG
                     ITADTFEVTEHLVDKLNRRILRDEEYKTIQDYNANRELVYRNVEKLQPFYNKEWIVNQ
                     GNKLTTDSPLMNKEVLSVTAMKGNDFITDLTDADHIMVHYADKTKDIFTISPKDSQVK
                     QVKEYSVAELGEVVYTPNMVVKDRADLISAIEGILSPIELQSDPIYQRLGRTGDNRVN
                     AIKNLYLEESFKEVKDHLNTFVTKLVENEDHQLNTDEAAKRALIKKIEDNKAAVLLGL
                     TYLNRYYGVKFDNFNIKELMLFKPDFYGKNVSVLDRLIEIGSKEDYIKGSRTQESFRE
                     VVAKNTLSGNLHDFLTYNMKLFTNETDINVWFKKAIENNAYVVEQPSTNPAFANKKYR
                     LYEGLNNGQHGRMILSLLNLKDAHLFMISTYNTISFSSFEKYGKDTEEKRESFKSEIN
                     KRAKEQVNYLDFWSRLATDNVRDKLLKSQNVVPTPVWDNHGAPGGWPDRFGHRKDKPD
                     YAPIREFFGRIDKYHDYKYGYGAYAYIFADPQPMDAVYFVMSDLISEYGTSAFTHETT
                     HVNDRMAYLGGHRHRQGTDLEAFAQGMLQTPAEHGHQGEYGALGLNMAFERSNDGNQW
                     YNPDPTKLQTRDQIDHYMKNYNEAMMMLDYAEAEAVLPKVKGDNSKWFKKIDRETRRP
                     MDRNKLSGPHQWDKVRDLIDAEKTTNLETIDDLVNNNFMTIHGNPGNKVFHPEDFGTA
                     YVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGYYGYEHGFIDYVSSKHQGAANKEN
                     NGLLGDDFIIKKVSNNQFSNLEDWKKQWYGEVLTKSKKGFAPIEINGTQISTYEQLKT
                     MFDEAVTKDLTELNDSSIKDKYRNTVVLKSKVFKALLKNTDGFSGDLFTAPQA"
     gene            complement(45268..45831)
                     /locus_tag="VQ497_000316"
     CDS             complement(45268..45831)
                     /locus_tag="VQ497_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HET2516680.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGVLFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFDLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            complement(45947..47638)
                     /locus_tag="VQ497_000317"
     CDS             complement(45947..47638)
                     /locus_tag="VQ497_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2516681.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            complement(47635..48372)
                     /locus_tag="VQ497_000318"
     CDS             complement(47635..48372)
                     /locus_tag="VQ497_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2516682.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
BASE COUNT        14595 a        10473 c         8498 g        14996 t
ORIGIN      
        1 ttttcgatag caatttattc ttccaataca cgaagaaaaa cctccacatt cagtggaggc
       61 aatctgtttt atcaatacaa ttttaagtca cgagggtcaa ctgggaaggt tgggttgtat
      121 ggattgtgac ggagcttaaa gtgtttgaca tcttcaatgg tctgagctcc agacaattgc
      181 ataactgtct tcaattccgc attcaagtgt tcaaagactt gacgcacacc gacactacca
      241 ccgagagcca agccataaat aacagggcga ccaatagcta ccaagtctgc tcctgaagcc
      301 aaggctttaa agacgtgttg accacgacga acaccagagt caaagacaat cggcacacgt
      361 ctatcaactg cttctgccac ttcttgaagc gagtcaaagg cagctggtcc accgtcgatt
      421 tgacgaccac cgtggttggt tacccagata ccagaagctc ctgcagcaag tgaacgttca
      481 acgtcctcac ggcattgtgg tcccttgaca tacacaggaa gaccagagta ttcagcgata
      541 aattctacat cgcgtggaga caagcgttgt ttagctgatt tgtaaacaaa gtccattgat
      601 ttaccagcac cttctggcag gtattcttca acaatcggca tgccaactgg gaagacaaaa
      661 ccattacgct tatccacttc acgattcccc cctacagtag catctgccgt caagacaatc
      721 gctttataac cttcagcctt cacacggtcc atgatgtggc ggttgatacc gtcatcctta
      781 ctaaagtaaa attggaacca atggggtgtc ccttgaaggg cttcagaaat ctctggaagg
      841 tcaacagtag agtaagagct ggttgtataa agagaaccaa actcatgcac accacgcgca
      901 gtcgccactt ccccctgttc atttgccaat ttatgagccg caacaggcgc cataatgatt
      961 ggtgaagata gtttttcacc tgcaaattca atctctgtac ttggattttc aacatcgcaa
     1021 agtgtatgag gaacgatgag tttgtggtta aaggcgcgga tattctctct taaagtgaaa
     1081 gtatcttccg ccccactagc gatatagcca aatgctgctt taggaataac ttgttgcgcc
     1141 attggctcca aatcataggt attgatgaag tctacatgac cttctgcatt gcttgttttg
     1201 tatgacataa aatgccctcc ttgattaagt aagcgtttgc tttttactac acaactataa
     1261 taactctttt ttcaatcatt ttcaagagtt ttgcttaaaa accatcttat tttttcatac
     1321 aatatactag ccctactttc cttaaactct aaactttagt ctatattaag tgaatcgttg
     1381 agattttttg ctgccaggca tttgaactac ttttttattt gcggattcta tctactaata
     1441 ctataaaaaa acaagtgaat aagacaccag aacatcctct aataggacct atggacttat
     1501 atcagtccca tatatactgt ttaagcaaga tactatccta gttgacgcta acttcctatc
     1561 ccacttatta attttttatt tatagtgaat ctgaacaaat caattgggga atccaagtca
     1621 atttctagta ctttttgaac agtttcagcg tactatcgta aaatgaaata aaacatgcac
     1681 aaattgctta gggaatttaa accaatttct aacaatgtct tagaaatcaa agtgtactat
     1741 ttcaacttca atatactaca cttcattttc tccttaaatt cagtctcttt ttgaatcaaa
     1801 aaatcccctg gtaagagcca gaggataaga atttatttca ttgttgggaa gagcaataca
     1861 tcacggatag ttgttgtatc agtgaggagc atgcagagac ggtcgatacc gattcccaaa
     1921 ccacctgttg gtggcatacc gtattcaaga gcttcaatgt agtcatagtc gatgcctgtc
     1981 gcttcatcat caccaagttc tttggcttta gcttgggctt caaaacggct aagttggtcg
     2041 attgggtcgt tcaactcagt aaaggcatta ccgtactcct tagtcatgat aaagagctcg
     2101 aaacggtcag taaagcgttg gtcttcagga tttttcttag cgagtggaga tacagctact
     2161 ggatgtccat agacaaaggt tggttggatt aaagtttctt caacaaactc ttcaaagaag
     2221 gcattgatga tgtgaccaac ctcagtgtag tgtttctcaa ctggaacttt cttctcagca
     2281 gcgatagttt tagcttcttc caaagtcatg tcttgccaga aatcgacacc agtaatttct
     2341 ctgatagcat ccaccatatg aacacgctta aatggttcgt taatcttgat ttcagtacct
     2401 tggtagttga ctgggccatc acctttgact gatttagcag cgtgttggat aatgccttca
     2461 gtcaagtcca tgatgtcttg gaagtctgca taagcttggt aaacttcgat agaagtgaac
     2521 tcagggttat gagtagcgtc cattccttcg ttacggaaga tacggccaat ttcatagaca
     2581 cgttccatac cacccacgat aaggcgtttc aagtgaagct cagtcgcgat acgaagcacc
     2641 atgtcaatgt tttgggcatt gtggtgggtg ataaatggac gggcagcagc accaccggct
     2701 tcattatgaa gaacaggtgt ttccacttca aggaatcctt tttggtcaag gtaacgacgg
     2761 atttcagaga tgatttttga acgagtgaca aagcgttcaa agctttcacg attagaaatc
     2821 aagtcaaggt aacgtttacg gtaaattgtt tcaacgtctg tcaaaccatg gaatttctca
     2881 ggaagaggac gaagagcctt agacaagtgt gtgatgtggg ttgccttgat agagagttct
     2941 cccatatccg tacgcatcac ttcaccttcg acaccaagga agtcaccaag gtctgctttt
     3001 ttgaagattt cgtagttttc ttcaccgaca gcatccttac gaacgtagat ctgaatctgg
     3061 ccttcgcggt cttgaaggtg ggcaaaacca acttttcctt taccacgttt ggttatcaag
     3121 cgtcctgcga tagtagctgt ttcgttttta tcgtgtaatt gttctttatc gaggttggca
     3181 tatttatctt ttaattcttg tgaatttgca gtacgttcaa aacgttttcc gaaaggatcg
     3241 attccttgtt cgcggagcgc agccattttt tcacggcgaa cgatctgctg gtcatttagt
     3301 tcttccatat gttctgtaga catgggatcc tcctagtttt actcaaaatt gaatacgatg
     3361 agtcattttt tgcgatactc ccattttatc ataaataagc aagaaattca cggataatct
     3421 ttaaaaacta aaaagaacct gacgctaaaa tcactggaaa gaagtgaaag tatctcccgc
     3481 cccactagcg atatagccaa atgctgcttt aggaataact tgttgcgcca ttggctccaa
     3541 atcataggta ttgatgaaat ctacatgacc ttctgcattg cttgttttgt atgacataaa
     3601 atgtcctcct taataagtaa gcgtttactt tgtgtattac aaaaatatct taactctttt
     3661 tcaaaacttt taaaatattt tgtttggaaa tttcagaaat tttatgtcta tgataaaaat
     3721 ccttataacg gcaataaaaa atagatatta tccaaagaag attttaagtg ctacaataac
     3781 tgtattattt ctagatggga ggttctattt ttggattgat ccattgttga acaatatcta
     3841 ccactatatc aaaaggcatt ctttctgacc ttgcatattg cagtttgggg aattttggga
     3901 tcctttctgc tcggtttaat cgttagtatc atccgacatt atcgaatcct tgttttggcg
     3961 caagtagcga cagcctacat tgaattgtca cgtaatacgc cccttttgat tcaactcttc
     4021 tttctctact tcggtcttcc ccgaatcggg attgtcctat cttcagaagt ctgtgcaacg
     4081 cttgggcttg tctttttagg aggctcctat atggcagaat ctttccgaag tgggctggaa
     4141 gccatcagtc aaacccagca ggagattggc ctcgctattg gtctgacacc tctacaggtc
     4201 ttttactatg tggttcttcc gcaagcaaca gcggtggcac tcccctcctt tagtgccaat
     4261 gtcattttcc ttatcaagga aacctctgtt ttctcagcag tggctttggc cgacctcatg
     4321 tacgtcgcca aggatttgat tggtctctac tatgagacag acattgcgct agctatgttg
     4381 gtagttgctt atctgatcat gctgctaccc atctcactgg tctttagctg gatagaaagg
     4441 aggctccgcc atgcaggatt cgggaatcca agtactcttt caaggaaata atctcctgag
     4501 aatcttacag ggattgggcg ttacgattga gatatccatc ctgtctgtcc tcttatccat
     4561 gatgttcgga acagtcatgg gaatcatcat gacctcccat tctagaatca tacgattttt
     4621 aacacgattg tatctggact ttacccgtat catgccccag ctggtgctac tcttcatcgt
     4681 ttactttggc ttagctcgaa actttaatat caatatctca ggtgagactt cagctattat
     4741 cgtttttacc ctctggggag cagctgaaat gggagacttg gtacgtggag ctatcacttc
     4801 tctccctaaa catcagtttg aaagtggaca ggcactcggc ttgactaatg ttcaacttta
     4861 ctaccacatc atcatcccac aagtcttaag aagactgcta ccgcaggcta tcaatcttgt
     4921 cactcggatg attaaaacca cttcattagt tgttttgatt ggggttgtgg aagtgaccaa
     4981 agttggacaa caaatcatcg atagcaatcg cctgaccatc ccaactgctt cattttggat
     5041 ttatggaacc attctagtct tatatttcgc agtttgctac cctatttcca aactatccac
     5101 tcacttagaa aaacattgga gaaactaaat gtctgaaact atcttagaaa tcaaggaact
     5161 aaaaaaatcc ttcggagaca atcccatcct ccaaggactt tctctagaaa tcaaaaaagg
     5221 gaagttgttg tcatcctagg gccatctggt tgtgggaaaa gtaccctcct tcgttgcctc
     5281 aacggcttag aaagtattca aggtggagat attcttctgg atggtcagtc tatcgttgaa
     5341 aataaaaaag attttcacct agttcgccaa aagattggca tggtctttca aagttatgaa
     5401 ctctttcccc atctggatgt cttacaaaac ctcatcctag gccctatcaa agctcaagga
     5461 agggacaaga aagaagtaac ggaagaagct ttgcaattac tagagcgtgt cggtttgctg
     5521 gataaacaac atagctttgc ccgtcaatta tctggtggac agaagcaacg tgttgcaatt
     5581 gtccgtgccc tcctaatgca tccagaaatc atcctttttg acgaggtgac tgcttcgctg
     5641 gatccagaaa tggtgcgtga ggtgctggaa cttatcaatg atttggccca agaaggccgt
     5701 accatgattt tagtaaccca cgaaatgcag tttgcccaag ccattgctga ccggattatc
     5761 ttcctcgacc aagggaaaat cgctgaagaa ggaacagctc aagccttctt taccaatccg
     5821 caaaccaaac gagcccagga atttttaaac gtctttgact ttagccaatt cggctcatat
     5881 ctataaagga gattcttatg aaactattca aaccactctt aactgtttta gcacttgcct
     5941 ttgcccttat ctttatcact gcttgtagct caggtggaaa cgctggttca tcctctggaa
     6001 aaacaactgc caaagctcgc actatcgatg aaatcaaaaa aaaagcggtg aactgcgaat
     6061 cgccgtgttt ggagataaaa aaccgtttgg ctacgttgac aatgatggtt cttaccaagg
     6121 ctacgatatt gaactaggga accaactagc tcaagacctt ggtgtcaagg ttaaatacat
     6181 ttcagtcgat gctgccaacc gtgcggaata cttgatttca aacaaggtag atattactct
     6241 tgctaacttt acagtaactg acgaacgtaa gaaacaagtt gattttgccc ttccatatat
     6301 gaaagtttct ctgggtgtcg tatcacctaa gactggtctc attacagacg tcaaacaact
     6361 tgaaggtaaa accttaattg tcacaaaagg aacgactgct gagacttatt ttgaaaagaa
     6421 tcatccagaa atcaaactcc aaaaatacga ccaatacagt gactcttacc aagctcttct
     6481 tgacggacgt ggagatgcct tttcaactga caatacggaa gttctagctt gggcgcttga
     6541 aaataaagga tttgaagtag gaattacttc cctcggtgat cccgatacca ttgcggcagc
     6601 agttcaaaaa ggcaaccaag aattgctaga cttcatcaat aaagatattg aaaaattagc
     6661 aaggaaaact tcttccacaa ggcctatgaa aaaacacttc acccaaccta cggtgacgct
     6721 gctaaagcag atgacctggt tgttgaaggt ggtcattaac tcttaaaagg gactggattt
     6781 taagctccaa tcccttttta agattttacc tataacatcc tgagtctatc taagatgttc
     6841 aatctgaaca cagtgtacat actttatctt ctattgcata tactttatca cataagatac
     6901 gaatatcctc ttcactatga ctagcaatca aaattgttgt ccctttttca ctagagagct
     6961 ttctaaacaa tgttctcata ttttctacac ttgatttatc caaggcattc ataggttcat
     7021 ctagtaaaag aatagaggga ttctccatga ttgcttgagc aatccctagc tttttcctca
     7081 tacctagcga ataagtttta actttctggt ctttttgctc atatagacca actattttca
     7141 gtgtatcatt gatttcctga ttaccaacta ctcctcgtat gcttgccaaa tattgtaaat
     7201 tcttaaagcc actataataa tttataaaac caggttcttc aatcaaagct cccaaattag
     7261 ctggaatttt tctctcagga acaatatttt ccccattgat taacacttct ccataagacg
     7321 gactatataa accagctatt aatttaaaca atacactttt ccctgagcca ttcgcaccag
     7381 taattcctat aatttccccc tgtttacaac taaagttaag gttttgaaaa acacatgtct
     7441 tttttaattt caactcaata ttttttaata taattatttc attcattcta taaacctcct
     7501 cttttgacga gtgaaataga aaatgctttg aaaaagaaag actaaaaata gcaactgaag
     7561 aaacaaatct ccccctacat ccccattccc tcgattcaaa atataaaata gataattagt
     7621 tcgatttcct acaaatagac caccaaacac aatcatgagt aaaaagaaac taacgcaagc
     7681 aaagttcatt tgtacattcg ctcctagtgc gtataacata aagcaaaaca agactaacac
     7741 acaagcattc aaagaatcaa ctataaacaa aactcctata tcccagtttg gtaactgtaa
     7801 cataaattga atcactagaa cttttaccat gattaaaatg agtccataaa gaaaacaggt
     7861 ccaaatctga tacctaatcc agagcttaat agaagaaaat cgaatcactt ccataaagaa
     7921 atcggatgct cttctacgat atacaactgc tagaaaaaat gtatgaataa taaaccaatg
     7981 aagcaaccat atagataaga ctaaaaaaga gttttgactc agattaggcc cttttaaaat
     8041 ttctataaca agtctatcat ttccttctaa ccaataccat aatcctaaac aaatcaatat
     8101 ccaaaaaaga acataacccc aaaagtactt ggtatagatt atcttattca acctccacat
     8161 gtgactactt aactttccca tttccaactt ttacactctc ctccagtttc tatgaacaga
     8221 aaatacagtc aatggaatta tgatagatag taatgtaagc caataaatca atgcatacgg
     8281 attggaattt accatatact gaaaaattgg caataatcca attatcccat tgttcaactg
     8341 ataaatccag aataaggatt ctacaaataa aaaagtaaac agaaatacta agctcatgtt
     8401 tgatttttta aaccaagaaa aactgctaat agtaagtaag gataaaaaga aatagtatgc
     8461 tatataagag aaaaaaaatc ctataaagaa actagcattg tttgcaatac ttataccata
     8521 aaattctctt aaaaaatcaa cctcctttat ctccaaagag aagctaaaac cattactaaa
     8581 tgcaattaga aaaatcaata aaaataaagt cgccgtccaa atccccgtac taagagctgc
     8641 taatttgaaa ctaaaactgg taaagtgctt aattgatttc agacgaatgc gacactccaa
     8701 cctattaaaa tagttattca tcaaataaaa aaagaacaat atatatgtga acggaaagca
     8761 atatactcca gtcgtcatat cttgaagtaa aactaaaatc cattctaata catttggatg
     8821 gattgaatat tggcgacagc gcaataaata tactgtacta gataaaacac aggatagcag
     8881 taatataaaa taaaccaata ctgataaaaa atcttttttg taaaattgaa caaattgttt
     8941 cattatacat agtcctctga atgtagaaaa aatgtaccat aaacaaccaa acaactaaca
     9001 aataaaataa aagcaagatg cccactaact aaggaaagac tgatatcttt ctgatatccc
     9061 aaagctaatg ttgtcacagg ttctaagtaa gatagcccta aaatagccca aaaaatacca
     9121 ccaaccatca tataggcaac tgggatgaaa atagctccta tttttttctt cactagcaaa
     9181 gcactagcta gtccaaaaat agagaacaca gcgccccaaa ctccatacca gagagtcgtc
     9241 acaagactat agagcaactg attagaatca aataattctt ttaaggcacc actataatct
     9301 ccaatataaa tttcctgata aggagtcact aaaagattaa ttcctaataa taataaatag
     9361 gggagaaaaa agactagaaa agaagaaata attgcagtac tacctacaat agccagatac
     9421 ttctttttag aaattcggca caattgtgct gttagaaaat gactctcagc atcctctatt
     9481 atctgactag aatagggcag tgtacagata agtgcagcta ctaggctaat cggtgaaaat
     9541 cctcgaatag aagaagcggc aaaaaaaatc gataaacctt caattttata aataccattg
     9601 aaagcaagga aatttcctaa actcatgcaa agaagggtca aaaataaaga catataaaat
     9661 cgaggtgatt gaacgactcg tacaagatta caaatgaaaa attccatcct tactcctcct
     9721 tataataaaa atagggtgta gcattctttt ttcatgctac acccacaatc aaccatcttt
     9781 aaggcttact ctgacaagta agttaataag aatctggact ccaagaacct gaagtatgaa
     9841 ttcttacatg atttccaaat tgtggcgcca tagctaatct agtaccagaa ccaatataat
     9901 tgtcaccacc tccattatag tacatgacaa tcctagagcc agaccccaat gaatataccg
     9961 gggtaatatc tgacccacta taggcgctac gaatagaggt atttaacctt ttaccgccac
    10021 cagtgctgtc actattatta attccagcag aggcgttttc tttctcaagc tcactagttg
    10081 tatccgaacc ccaacgcggc aacgttaaat cataatttcc aatctgtcta gctaaaactg
    10141 tagggagaaa caaactagat gcaattgtaa ctacgcaaaa aaggaatata tttttcttct
    10201 tttcatgaca tcgtcctctt aagaagtgat aaacaagata gttgattgat caatttgata
    10261 catattacat tttcattata tcaataaaag aaagcgttgt caattatttt atttaaaata
    10321 ttgtcatatt ttaaataaaa tggtaaaaga gactcggtag cctcgtctct ctttatctta
    10381 ctgacctaac cttcttgcca attttcttgg tcttctttaa agcgttttag aagatcaagg
    10441 ccctctggcg tgatgtaacc ttcttcttgg gctagatgga taagctctct atagtttgaa
    10501 agcgtcacaa gtttaacacc agcatctgca aagttcttat ctgcttttgg caattggtag
    10561 ctgaaaatcg ctacaactcc aagtacatct gctccttctc gcttggctgc tgctacagct
    10621 tcaagaactg aaccacccgt tgaaataagg tcttcaacca ctaccatttt ttgaccttga
    10681 gctacgcgac cttcgatttg attaccagct ccgtggtctt ttggtttgct acggatgtag
    10741 gcaaaaggca agtccatctt atcagcaata atggctccgt gtggaatccc tgctgttgca
    10801 gttcctgcaa tcacttctac ttcaggaaag gcttctttaa tagcttccac aaaaccattt
    10861 tcaattaggg tacgagtttc tggataggct agtgtcacac gattatcagt gtaaatcggt
    10921 gacttgatac cagatgccca agtgaagggt tcctctggtt tgaggtaaac ggcttggatt
    10981 ttcaagaggt ggctagcgat atctttagca agtgtcatgg tattctcctt ttatttttct
    11041 aatctagttc tttaattcca gttctgtgtc cattcatcct tgatggcatg ataagctgca
    11101 acaggatcct cagcttgggt aatgggacgt cccactacga tatagtcact gccgatttga
    11161 taggcatcag caggtgtcat gactcgtttt tgatctccaa ctgcaacacc agctggacga
    11221 atccctggtg tcagacagat aaaatctgga ttggtagcct gcttgatgac ttgtacttcc
    11281 tgagccgagc aaacaacacc atccaagcca gcttcagctg tcttcttggc atagtgaatc
    11341 acagactctt gcagactggt ttggatattt tgaaactcct gcatctgagc ttctgacgtt
    11401 gatgtgagct gagttacagc gatcaatttg gcttgactcc caagaccttc acgcgccgcc
    11461 ttcatcatct ctacaccacc agccgcatgg acattagtca tatcgacacc aagctgagac
    11521 aagaccttca tggctgactt gactgtatta ggaatgtcat gaagtttgag atccaaaaag
    11581 acactatgac ccaaaccttt taagtaggac acaatctcag gccccgctgc gtaataaagc
    11641 tccatcccta ccttgagata aaggctttct tctgctggga aaagagctaa aaattccttg
    11701 accgcctcaa aactaggaaa atcaagagca atgattggac gatgttctcg cataggtttc
    11761 tccttttacc tttactagtg agagagtctg gccaacagaa gccacgaaaa aaggaacctg
    11821 ccaacagcaa ggttccacga aaaatgggta gtctccaccc tttcaccgtc taaccttagc
    11881 tgcctctctg gactgcttta aaggttttct attattctat tatttctact agcacttgtc
    11941 aagtaaaaac ttagtcacgt atgtgttgac tgctattgaa cacctcacaa gactctttcc
    12001 aaaagaatag ttaaagactt tcacttagga cagagaaaaa gagtttgctt gttacccttt
    12061 aattgaacaa atgggaattc tgttttattt tgcggatgcc tattcatctt ggcaaagggg
    12121 aagtaatgag aatgcgaatg gacttcttcg tgagtatttt cttaaaccga tttggcaaca
    12181 attaccgaaa aaatccttaa tggtgctttg ctagcttatt aatcaccgtc cccaaaaata
    12241 cttggattac aagacaggtt ttgaagcact tatagaggaa atttaaaaat gttgcatttt
    12301 ttattgcaat ttatcagtta tcttatcacc caaaacttct gcaattagta tgagcactat
    12361 tgttattttt cttttttcgt acactcaaat caaaatatag caataagact attctgtaga
    12421 attgctgtca aaaagatcag ttcatttttc cttcccactt gtgtttataa tcattaaaat
    12481 aactcaatta agaaattttc tttttcctaa tagttatcgt aattaaagtt aatagaagaa
    12541 caatccaaat aagtagtgtt agattaggaa ttagagtttt attccatgaa aatccctccc
    12601 attttgtgaa aaacttagta aacagtccca taaaactata atctgttatc ttcgcaactg
    12661 tcttatccaa accagatagc atcggtaaga aagcaactaa aatcatagca ggaagactga
    12721 taacctgagc tactacttgg ctcttcgcgg ttaaacctat caataaatag aataaaatga
    12781 tggataaaga ggttagaaga acgattgtaa tataattaaa agtatgtaca attgtaactc
    12841 ctaaaataag aggagtagtt cccataatca caaaagttag caaaaaagga agaaacatcg
    12901 ttgataaaat gtattcggat cctttaacac cactcaacag aagagtttgt aaattgtact
    12961 tttctttttc ttcagacaag ataatagtta taggacttcc aacagccaaa gccaaagaaa
    13021 aaggtaaaca catcatcaaa agaactaatg cctgttgatc gttgaccttc ccctgtgttt
    13081 ccataagata tttataaaaa tatgtgaaag caaaaggcac taaaacttgc aataaaatac
    13141 tcttattgga aaggataatt tgacaacgca accataccaa agccagaaac cgtttaagca
    13201 tttagctttc ctcctgttaa tgtgataaaa atatcttcta aagtaggctc acatgaatga
    13261 attgaaaaaa tattttccag atcagcctga gatacctgtt ctagtgatgt aaaatcaaaa
    13321 gttatctgat tcccattata atctgtaacc ttaatctttt tatctttatt atatctttga
    13381 atgagttcag aaggagctcc ttgctcaact aatttccctt tatttaataa ggcaacataa
    13441 tcacataaaa gagttgcttc attcatatca tgagtcgtta gaaaaatcgt tgtccctgct
    13501 gttttcaatt ctaaaattaa ctcatgaatt gttcgagaag ttgtgggatc tagacctgag
    13561 gtcggttcat ccagaaagag tacagcgggt ttgttgataa gagctcgtgc taaaagcatt
    13621 cgttgcctca ttccagtgga taattttcct gctaccatct tgcgactatc atataatcct
    13681 actcgcttta acaaattatc aacacgtgat ttactaatat tataaaattt actataaaaa
    13741 agaagattgt tatacagaga cattttctca taaaatccac ttgtatcgct aaccaatcca
    13801 attctcttta attcaccgct tgttaaattt tgagattttt gtcccaaaat aatagattgt
    13861 cctttatcgg caaggaactg cccagtcaga atattaatcg ttgtggtctt tccagaacca
    13921 gatggtccta aaaatctaac cttctttaac agtgaagctt atattattta aagctatttt
    13981 atcaccaaaa ctttttgata aatggctaac ttcaatcata ctcattgcac ctcctttata
    14041 ttctacattt ttacttaaca tctatttact tatctttatc aaataaacgt cttaaaaaaa
    14101 acaataacat taagcatata accgttagca agattgtttc aatattattg ataatacgat
    14161 acattctcaa gcagtataaa ataattatat atatgcaaat gctcattata cctatttttt
    14221 taaacatact aagcctcctt gctaaataat aaagttatat aataactaag acacactata
    14281 cctaacataa taaatatcca ctggtaactg aataaatgtt ctaaaattaa caagcaaaaa
    14341 taaaatctca aacgatgaaa aaagccatac tatattttcc aatgatgttt gaatattttt
    14401 attgtagtaa gcataatata tagcaaaaat aatacccaag aacaatacta ttaattgtgt
    14461 ttctataaca gtgaactgcc caatagcaga taaaaaagct actcctcctg ataataagca
    14521 aaaacaaact gaacgaataa atgttttcgc tctaatattg ttatctttca ctttattatt
    14581 tttttacaaa ttggacattc tccgttttcc agaacatatt tttcttgact tagagcatga
    14641 tttctcatat tataaacgta agcgttatct attaggattt cattataaca tcccagaaat
    14701 attttaatta ttaaattagt cattatccct cccaaaatag caggcatctc tggtgtagtc
    14761 ggccactttg tcttattaga taaagtgtac ttatttatat ctttgtttat attccgacaa
    14821 ccaaagcaag aaccgatggt agggatatat attatcagta ataagatagc atccattaaa
    14881 ccctcctgat atgtaggata tcttatgcga tacagcaaat tgattgacaa tttttataat
    14941 atcaatgggc gtatcaattg ctttaacgat aaaatctata ctcccatatt ccgcaacaat
    15001 tttttctaat tcttctactg aagaaacttt aatagaaata ggtactacct gaacatctga
    15061 attatacttg tgtatttttt cagaaagaac attaatcttc tccttaccaa catccgactc
    15121 tgtataaaga atttgcctat ttaaatttga tggttctatt atatcgtaat cagcgacaat
    15181 aagtttcctc actccataca tacttagact tagagatata taacatccta aggctcctgc
    15241 tcctaatact aatactgttt tattctgtat tttattatac tcttcattat aaaataacga
    15301 atagtaacct ttagttctag aatatctact atcaatatct atctcatccg aaattaaaat
    15361 ttgttcattt aatagttcaa atgttagttt actgcctaat acttcttcta tctctaatag
    15421 ttccttacaa ctattaaata gatcgttgat aaattcttgc gaaatttgag gttctatagc
    15481 tatttcccgc atactccgac cacgcaaata cattatacct tcttttttat aagcaaaaac
    15541 ataactattt acgtagaaca tatttcgtcc ttcctctcat atataatata gaaagcagta
    15601 tagaaacaac cacccctcct atactacgaa caataatatt aggtgtcaaa aactgtaata
    15661 gttgactcaa agggaaataa ataataacaa atgctattaa aagttgaact atatattgaa
    15721 aattcaagtt attattgtta gaattcaagt atcttaaaat aataccacta tcatactgaa
    15781 aaaaaggaat tgaattaata aaaatcatac ctaagttaat gataaaaaat aagagtccaa
    15841 gcaatttata cgatgtgaac aataaaacaa gtaaacctgt aagtgataaa atcgaatttg
    15901 aagcaattcc tgctaacata attttcaaaa attgtttttt attttttaag aattgcaccc
    15961 cacttaaatc tacaaaaaaa agccaaatta aacaaaagta gcattattcc acaatttggg
    16021 atctcaattt gataattatt agcaattatt gcatgactaa attcatgtat cgttgcagac
    16081 ataataacaa caagcaacca aaataaaaga atgtttagcg ttattggaaa gttttggaaa
    16141 ttagtcaaaa tatccgccct ataaatatac ataataaaac attgcaaaat aagagccaat
    16201 gggaaaatac caaataggat tttttcaaaa ttcttattaa atcttttata gtttaatttt
    16261 tcagataatg ttatattaaa gtcccaagat attttaaaaa tatttatctt ttttcgtgat
    16321 ttcatagaat ctataagtcc cattttacta agaacaatat aaaatcttaa ataatatttc
    16381 ttccctacta acactaataa gtccgatatg gtaaattctt gacctattag ttgctctaac
    16441 catttttttc ctgtttccca actaatacat gtttcttact ggatggtata tatagatgat
    16501 actgtccctc ataaggagta ggtaatatct taatcttatc aacaattctc ataacaaacc
    16561 taacctttaa taaataaaag aggggctaag taaaacatac tctagtccct cagtccgcaa
    16621 tcaataaaat aatgactaat tctattaatc tcttgctgca agactaatac ggaaaattca
    16681 aatgactgtc taaaatgaaa ttgaaaaagc ttgatgttag taaatgaaca aacttcaaca
    16741 accagcaaga gcaaggattg ctaaaccaaa tccgatacct gttgcaagtc cagctgaaaa
    16801 atcactaact ggtactgaaa tttcttcaat ttcctcaaat aaaaagttat cttgcatgat
    16861 tatcactccc tatcttttta acaacaggct attccaatta agagaactac cccagttcca
    16921 agtaaaactc ccccaaaaat tcaccatctg taggggctac gatttcttca atttcttcaa
    16981 ataagaatgc ttcatctgac atatgtgtta ccttcttcat aaaaaattat tttgtaatcg
    17041 attacattgc aaatgtaaca taaagaaaaa cagatgtcaa atattaaacg taagaacatg
    17101 gtcactaaag aactataaga gaaaaggtaa acctagcgac gcgatgagcg ctgggtcgtt
    17161 tgatttcgat tgctctcttc ctcttgtttt ttctgttctt cttcctgttt tttctcggct
    17221 tccttggcct cttgtttggc tttttcctca gcctccataa ttaatttatc cgccacagtg
    17281 taactgtaaa ttccagcttc catgtcgacc acactcggtt ctgacaattg tggcttaatc
    17341 ttactgtaat atggcagttt cttactcatt tcagatagag gaaccaagac ttcgtccgaa
    17401 tcattcatgg tcaatcgaat taaatcggat gtcaccttgc ttggggctaa ttccaccttt
    17461 tggatagccg ccttgagttc tgggctaatt tgagcaagtt ctgagacaaa aaccttgatt
    17521 tgttcactat cattaaagag aactgataaa taagtttctg gtaaactgtt cagactcaca
    17581 gaactagtct caagctgacc actggaaaga ataggataat gattttcacc agaaatatag
    17641 taggccacaa tatcatattc cttgacctta atagtgaact tagttggaaa ttgatagaca
    17701 agttgagctg attcaaccca atagttagac ttaatctgct tttcatattt tgccttgtct
    17761 agcagaaggt taatcgtata atccgaatcc tgaatgcctg aagcctgtcg aatatcatca
    17821 gctgtagttt gcaccgttcc ctcaacacga atatctttca tggtcgcata aggactgagc
    17881 aagtaggcag agacaatcaa taaaagcaga cttggaaata aaatcgtgaa ggctcgcaag
    17941 atatggatac caggaatctt tgctttggct ggtttttcct ttgtagcctt tttagaaagc
    18001 tttttatcct gttcctcctt ctctttagac tctggttctt ctttctcttc tttctctttg
    18061 tcagcctctg aggatgctac tttttcttca gactcttcct tagctgattc tgaatcttcc
    18121 tggtctgttt cactctcctg gtcctgtttg tcctctgact tctcagattc ttctcccatt
    18181 cgagcttgtc tttccttttc cttctcctca gctagagccg cctcttcttc agccttcttt
    18241 tttagatatt cttggtttcg tttctgccat tctgataact ctttcaattc ttcgaggatt
    18301 tctttgtcct catttttctt atcttttgac atttactttc cttatgataa atcttttttc
    18361 aacaattgat aaaaatctgc tagagatttc aattccttag aagccttcat cttagcttgg
    18421 taatcttcct tgtgacttag taagtgagaa agcttctctt ccaaactatc caaggtcaaa
    18481 tcgctttctt gaaggtcttc tgcatagcct ttttttacaa agtaagctgc attttcaagc
    18541 tggtcaccac gactagcttc acgaccaagc ggcacaatga catgcaattt tgctatcgcc
    18601 aagagctcaa aaatcgtatt ggcaccacct cgtgtcacaa caatatcagc caattccatc
    18661 aagggttgat agagatcggt cacatagtca acacgaaaaa gattttggct caactcattc
    18721 aggctagaat ctccagttag attgataata ttgtagcgct ctgttagttc tttcttatgg
    18781 tctgtcacca attggttaaa gacacgagca cctgcagaac cgccaacaaa caatacagtc
    18841 ggcaatttat gattaaagtg gctttgaata tccaccaatt catctggttc tggatttttt
    18901 tgatctgaaa ccttggccac tgctcccaca tgctcaacct tagacaaact cgaagcttgc
    18961 tcaaaggttg aatacatctt agtcgcaaat ttataggcga ttttattggc caagcccata
    19021 gacaggtcag attcgtgaat aaagacaggc actcctgaca cacgcgcagc gataacaggc
    19081 ggtactgaga caaagccccc ctttgaaaaa agggtctgtg gacgaagtcg caacatgata
    19141 aagagcgatt ggacgattcc ccagcaaact ttgaagacgt ccagcatatt ttgccaagag
    19201 aaatagcgac gcaattttcc agtcgcaata gaatggaagg tgacatccaa acctgactta
    19261 aggatttctt ggtgttcgat accacgcttg tccccgatat agtggacttc ccaaccatct
    19321 tcgatgaact tgggcattaa caaaagattg agggtaacgt gtccaaccgt ccccccacct
    19381 gtaaagacaa tttttttcat attattcttt taactccgct actgtgtcga taaagaggtc
    19441 gccacgtact tcaaagttag catacatatc ccagctggca ttggcaggac taagaagaac
    19501 cacatctcct tgagtcgcaa gctcataggc cttgcgggtc gcatctgcaa tatctgtcgc
    19561 ctccacataa gcgacaccag ccttgtctgc tgcccgtttg acacgttctg cagattgacc
    19621 caggatgacc atcttcttga gtccagtaat gtctggcacc aattcgtcaa actcattgcc
    19681 acggtccaaa ccacctgcaa tcaagacgac cttgctgtta tcaaatcctg acaaggcttt
    19741 ttgagtagcc aagatattag ttgatttact gtcgttatag aatttaacac ccttgatgtc
    19801 atccacaaac tggagacggt gtttgacacc accgaaggct gaaagagttt ccttgatggt
    19861 ttgattgtcc acaccacgaa gcttggctac agcaatagtc gcaagggcat tttccacatt
    19921 gtggctacct ggaacaccga tttcattcgc tgccatgact acttcaccac ggaagtagag
    19981 ttgaccatct tccagataag ctccatcaac cttttcaagt gttgaaaatg gtacaacagt
    20041 ggcttctgtc ttggaagtca agtcttttgc caagtcttga ttaaagttca agacaaggaa
    20101 atcagctgct gtcatcttgt tctggatatt ccacttggct gctacatatt ccgaaaatga
    20161 cccatggtag tcgatatgag ttggcatgag gttggtaata accgcaatct ctggatggaa
    20221 ttcttgaaca cccatgagtt ggaaagaaga aagttccata acaagcgtgt ccttatctga
    20281 tgctatttga gcaacctgac tagctggata gccgatattc cctgataaaa gaccatgttg
    20341 cccagcagca gtcaaaactt ccccaatcat agtcgttgtg gttgtcttac cgttcgatcc
    20401 tgtgatacca ataatcggtg cttctgaaat caaataagcc aattccacct cagtcaagac
    20461 tggaattccc ttggccaaag ccttttcaat catgggattg ttgtagggga tacctggatt
    20521 tttcaccata agggcaaact cttcatccaa gagttccaaa ggatggccac ctgtaatgac
    20581 cttgatccct tcttccagca aactttgggc agctggattg tcctcgaaag gtttcccatc
    20641 atttactgtc acaatggcac ctagcttgtc caacaaacga gctgcagatt caccagactt
    20701 ggccaaacct aaaacaagga ctttcttatt tttaaattga tctattactt tcatgtctcg
    20761 aactccattt ctactcctac tattttacca tttttatgga aataaaaaag ccacaaagtg
    20821 tgtttgtgac tctttcttct aactgaatct taccatatca tctatgtgat aaatcggtaa
    20881 ctcgaatgac ctgatccact tgttcccaaa taagaggatt gtgggtcgca ataataatgg
    20941 tccgattcgg attttttaaa gattctagga tggaaagtaa ttcctcagag tttttggggt
    21001 ctaaggaagt ggttggttca tctgcgagga tcaaaggtgg atcctttaaa attatcttcg
    21061 ctaatgcaac acgttgtgct tctcctcctg ataactcaaa tataggttgc ttcaaatcca
    21121 aataagagag gtttacacag tttagagctt gtttcatcaa agagattttc tctttttcct
    21181 tcaacttttt accaactaaa cccagattga gattctcttt gacggtttgg ctttcaatta
    21241 agccaaaatc ttgaaataag tatcccaagt aatctctaaa gaaaacagaa ggcttgatgt
    21301 ccttaagaga agtaccatca tagatgattt gtcctttgtc atatggctct aatcgtccaa
    21361 tcatattcaa gaatgttgtc ttaccacagc cacttgtacc gattaaggca taaattttcc
    21421 caccttcaaa atgaagattt gtatctgaaa atagctgacg gcttccaaat tttttagata
    21481 tattctttag ttcaatcatc ctattttcct ttcataattg tcatagaaac acgagattct
    21541 ttctgcgctt gacggtaaag cgtcagaact gcactagcta gaaagactaa taaagtgagc
    21601 aagccaatca ccaagtctcg actgcttaaa ataaagagac tagcaccaaa tacaaaactg
    21661 gcaaattgac taaccatata ctgagcatgt gtttcaaaaa atcgtaaacc tgaaattcgt
    21721 ttaatcaaaa tatctcggcg gaattgctcg aaatatagaa gattgacaga ataaaatagt
    21781 aataaggaac tagctatccc cacaatagct cctaagatta aagttgctgt ttcagtttga
    21841 acttcattat aacgagttag ataaacactt cttccttctt taagatagga tacttgctca
    21901 taaattccag ctttcttcaa gagttctagc ccactctcat atcctttgat aaagagttgt
    21961 tttccagcat tgatagacca actagataag gatataaaac tatcacctgt agaagtcggc
    22021 gtgaatacca ctaaaatcgg atcagtcaaa tactgagtag atacgggatt ctcaccgtta
    22081 ttataaacaa accgcttttc tcccattgaa agataactaa cgtgcgcttt catctcataa
    22141 tccaaaggag cacttgcctc ctcaccagat tttccataat aactcaatct ttcttcaaaa
    22201 actttcttaa gttctgcttc tcgagagcgc aaatgttctg ggagcaagag gataaactca
    22261 cctttttgga gatgggctaa cttctgtttg gtctcagcat ctaccacgac cttttccttg
    22321 tccaaataac tgggactaac atagagcgta ttagcatctg gactataggt atccagtgtc
    22381 tctccctgtt cattttttcc ttgtggattg gcaaaatgga gcagattatc ctttacataa
    22441 agagcttgtt cttcttcgat tgcttccttg gcaaaggcat accacttgct ctgattttct
    22501 gtatcttttc ctctatcacc taagccaaaa gaaatttgat aatagtctgc tctatcctgc
    22561 catgcttgtt ttgaaatttc aagttctttc aatcgttggt aagacatcaa acctgtctta
    22621 acagcgtagc ctactgtaaa gacagcaacc aactgacaca atagggttaa aatcatcagg
    22681 cgtttaaggg gtaatctccc cttaataacg ggaactaatg ctttgtaact caaactcatt
    22741 agataaagaa gcattagtaa aattgaaatc gccaataaaa acaacaaata gaaactaatc
    22801 ccaaaaccat aggtggctaa caagatagga taaaacaaac cttgactaaa aagaacgact
    22861 cccccaccta ggaaggaaag gagggctgat agaaggagcc atttgatatc agtagataaa
    22921 gaatgcccca tgatggataa gagagtctga ccagaaaaga gttttatacc tgctgctctc
    22981 atttccttaa tccgagtgat aatcactaaa gcaaagaaag ataagccaaa tattgctaaa
    23041 ctaattaaaa taaggggatt tagtaatatt cgaaaagcaa gaaaataggg cggtatcttt
    23101 cggtcagcac ttgctttata acccaaatct cctaatttat cggcaagctt ttctttcgtc
    23161 aaggagcctg acaaaaggag ataactattt agcggatcac tacgttcacg actttcttgg
    23221 ctagcttctt ggaattcttt tggtaaagtt ccctgaccat aagttgcata agtaaagtga
    23281 gtcgtcccat ccttactcgg ctctacaatt cttctagcta ttaaactctg ttctgagttt
    23341 gcaaaattct ccaattcctg ttcaaatacc tcacgcgtcg gttcctgagt atcttttttg
    23401 acacgaagta aagaaacgga atcatagctt gcatataaat attgtggcgc acgtaagaca
    23461 ataatccaag caaggaagaa gctgagaaaa aaagttgata ataatatgaa tagtttcttc
    23521 atagtagact ccttgtaaac aaaattcccc ctgtaatttc ttacaagggg aacgatttaa
    23581 atcaatgaac gattagtcat aatcacagta aaatgctact tgttctcccc atttagtcca
    23641 aatccatgca taggaggttt ttccagcaga agcttctcct ttatctgaat gactattcca
    23701 tctacttaca agagaagaat aatgatattt tttaccatga tagtaatttg aaaaagccca
    23761 accacctcct gaaccttctc catacgtcca tactcctcca tctggatatt gtacagcagc
    23821 tgatgcagct cccaataatg taaaacttga aataagagct agagcaagta atctatgttt
    23881 tttcgttttc attttatttt ttctttcaaa aaaagcacac cttgagcaac aatgcaacaa
    23941 aataaaccct cctctctctt ttgttgaaac cgctttctta tgtgataaaa atatcatttt
    24001 tattatttgt tgtcaaggaa aaaatcgaat tttttagata ttttactaca ttgcctctat
    24061 gaataatatt atatagtagt tttattttaa aataatatgc aaccagtact aaccaaatat
    24121 aaaatagatg ccattaacga attttattca agtttttccc attcatacta tccaagcaaa
    24181 agagatgatg ttatagtaca aaacaattca aactattgta aaattcctag caaaaaagag
    24241 agccaaaact ctctttttta tcttctttta ctttttttga ctggcatgag tgtaatgtct
    24301 ctaacactaa agtaagctag gatcaacatg gctattgcta ggaatatttc tgttggtaat
    24361 tgaaaaattt tcagaaaaga tagaaccaat aaaatcaaga gtgccactaa aatacatacc
    24421 atagcgacga tattgactgt ccctttaatg ctttctggtg tcgcaaatac atagagtagg
    24481 agcagtaaaa ttcctaggac taaatagacc atctttctct ctttctagct cttattcagc
    24541 tgattttttc ttcttgttag ctttctcacg ctctgctttg ttaaggattt gtttacgcaa
    24601 acggatagac tcaggcgtta cttccatgta ctcatcgtcg ttcaagaact caagagactc
    24661 ttcaagtgtc aagatacgag gcgtcttgat aacagctgtt tggtccttag tagctgaacg
    24721 aacgttggtc atttgttttg ccttcgtgat gttaactgtc aagtcatttt cacgagagtt
    24781 ttcaccgatg atcattcctt cataaacctc agtacctggg ttgacaaaga tcgtaccacg
    24841 ttcttcgata gacatgattg agtaagttgt agccttacca gcatcgatag aaacaagggc
    24901 accacggtga cgtccaccaa tttcccctgg aatcaatggc aagtattggt cgaaggtatg
    24961 gttcatgata ccgtaaccac gagtcattga caagaactca gttgagtatc caatcaaacc
    25021 acgcgctgga acaaggaaga ccaaacgagt ttgaccatta ccagttgaaa tcatatccaa
    25081 catttcacct ttacgttcag aaaggctttg gataacagac ccttggtatt cttctggagt
    25141 gtcgatttgt acacgttcaa atggttcaca tttaacaccg tcgatttctt ttacgataac
    25201 ttctggacga gatacttgaa gttcatagcc ctcacgacgc attgtttcga taaggattga
    25261 caagtgcaat tctccacgtc ctgaaacagt ccatttatct ggtgaatcag ttgggtcaac
    25321 acgaagggaa acgtctgttt gcaattctgc ctgcaagcgt tcttccacct tacgagaagt
    25381 tacccattta ccttctttac cagcaaatgg tgagttgttg accaagaaag tcatttgaag
    25441 agttggctca tcgatgtgta ggattggaag agcttctact gcatctgtcg gagtgatggt
    25501 ttcaccgaca aagatgtctt ccatacctga aacggcaatc aagtcacccg ctttggcttc
    25561 ttggatttca cgacgttcca aaccaaagaa accgaagagt tttgtaacac ggaagttttt
    25621 agttgtaccg tcaagtttag aaagggtaac ttggtcccca accttaactg taccacggaa
    25681 gacacgaccg ataccgatac gtccaacgaa gtcattgtag tccaaaagtg acacttggaa
    25741 ctgcaaaggc tcatctgagt tatctactgg agctgggata tggtcgataa tcgtgtcaaa
    25801 gattggtgcc atagtcgctt cttggtcagc tggatcatct gacaatgaag aagttccgtt
    25861 gatcgctgaa gcataaacca ctgggaaatc aagctggtcg tcatctgcac caagctcgat
    25921 gaaaagttcc aagacttcat ccactacttc tgctggacga gctgatggct tatcgatttt
    25981 gttaacaacc acgattggga caaggtcttg ttccaaggct tttttcaata cgaaacgagt
    26041 ttgtggcatg gttccttcat aggcatctac gaccaagaca acaccgtcaa ccattttcat
    26101 gatacgctca acttctccac caaagtccgc gtgtcctggt gtgtccataa tgttgatacg
    26161 agttccgttg taagcaacgg cagtattttt agcaaggatg gtaatcccac gctctttttc
    26221 gatatcgttt gagtccatag cacgctctgc caattcagta cgtgcatcaa gcgtttctga
    26281 ttgtttcaat aattcgtcaa ccagggttgt tttaccgtgg tcaacgtggg cgataatcgc
    26341 aatgttacgg atatcttctc ttaattttgt catgatttcc tctataatat tcaaaattta
    26401 ttttctaact gaacgattat accataattt caaataaata acataactca agcaagtgta
    26461 aatgttttca ctccgctttt cttttcacgt caagcctttt caaagcaagc gacttatgat
    26521 aagataggca cagtatgcgt ttagataatt tattagccca agaaaaagtt agccgaaagg
    26581 ccatgaagca agcactcctc agaggggaaa ttctagtcga tggttgccca gcccgatccc
    26641 tagctcaaaa tatcgataca ggactacaag aactcctttt tcaggatcga atcattcaag
    26701 gctatgaaca cacctatctt atgcttcata agcctgctgg tgtcgttaca gccaacaaag
    26761 acaaggaact tccgaccgtc atggacctgc ttccatctga catccagtct gacaagctct
    26821 atgccgttgg ccgactggac cgagatacaa cgggactcct cctcttgacc gataacggtc
    26881 ccttgggctt tcagctcctc catccccaat atcatgtcga taagacttac caagtccagg
    26941 ttaatgaact tctaacacct gaccatatcc aagcctttca aaagggaatt gtctttttag
    27001 atggtactgt ctgtaaaccc gcaaaactag agattctatc tgcaagtccc tccctcagtc
    27061 aagcctctat caccatttca gaaggaaaat ttcatcaaat caagaaaatg ttcctctcgg
    27121 ttggtgttaa ggtgactagc ctcaaaagaa tccaatttgg ggacttcaca ttgaacccag
    27181 atttagcaga aagtaactac cgccctttga accaaaaaga gttacaaatc attaaaaact
    27241 atttagagat gagtcgataa aacaaaaaaa gctttaaaac taaagctttt ttcttttatt
    27301 taccgaaaaa ttaaggcgat tgctacaatc cagttaacta cagaaatcac aattcctaag
    27361 atattaagaa tcttttctat tttatagtct aattgtgact ctttttggta tgaaatagcc
    27421 aagaccaatc ctatgatacc caaaatcaag cctacaattg gaaataacaa accaagaata
    27481 atcgacaaga tacccacaaa aagtggattt ttcttctttt ctttcatgtt ctaagaactc
    27541 tttaaatttt atacaaatta attatactat aaaacaatag cttcatccta tcattcgact
    27601 aatttggaaa taaggttagc tagtcttcac tttccctttc caagaatcca agccataaga
    27661 aaggatataa atctcagaaa aaccttgttt tttcaagtaa agagctgcat ttgtaactcg
    27721 ttgcgcacgt tggttctcgt agagaaggac aggtttatct ttacgaaggg ctgcaagact
    27781 agttttcaac tgacttgaag gaatattgcg tgcaccaagg atatgttttc tgtggaattc
    27841 tgctgggtcg cgcaaatcaa tcaattgacc cgtacgaatc aaggcttcaa actcctcatt
    27901 gtccacaatt ttagccgcac gacgaatacg aagatagtta aagcccatcc acgccaacat
    27961 tgctagtata agtgcccaca aaatccaagt aaccattagt tcttttctcc atttttctca
    28021 atataatcca attctacctt gtgctctctg cgaagaactg cttctgcctc tagatagtct
    28081 aatttatcca tcaaccctgc atcgtaaatc cgagatagtt ccaacttcat cagttcaata
    28141 tcatataagc gttttcccat gtaaacaata ataccaaatc gtttgaggaa ttgctgcaca
    28201 tcatagaatg ttttcataag actcattcta gcaaaatttt gtgttttttt caagaagaga
    28261 ctcacacaat gctccttatt ttcctatctt ctttggcgat tctaaggcaa gtgtggtaca
    28321 ataaaaacat gagaattcaa caattacatt atattatcaa aatcgtcgaa actggctcta
    28381 tgaatgaggc agccaagcaa ctctttatca ctcagccaag tctttccaat gctgtgagag
    28441 atttggaaaa tgaaatgggc attgagatct ttatccgcaa tcccaaggga atcaccttga
    28501 cccgtgatgg gatggagttt ctctcttatg cccgtcaggt tgtcgagcaa actcagcttc
    28561 tggaggaacg ctataaaaat cctgtcgccc accgcgaact ctttagcgtt tcgtctcaac
    28621 actatgcctt tgtggtcaat gcctttgtct ctttgctcaa gaaaagcgat atggagaaat
    28681 acgagctttt ccttcgtgaa actcggactt gggagattat cgacgacgtc aagaacttcc
    28741 gcagtgaggt cggggtcctc ttcttaaaca gttacaaccg tgatgtttta accaagatgc
    28801 tggatgacaa tcacctacta gcccaccatc tcttcacagc gcaaccgcat atctttgtca
    28861 gcaagaccaa ccctctggca aagaaagaca aggtgaaact gtctgatttg gagaatttcc
    28921 cttacctcag ctatgaccaa gggacgcaca actccttcta cttttcagaa gagattcttt
    28981 ctcaagaaca ccacaagaaa tccattgtgg tcagtgaccg tgccaccctc tttaatctct
    29041 tgattggttt ggatggttat accattgcga cagggatttt gaacagcaac ctaaacggag
    29101 acaatatcgt ttctatccca ctggatattg atgacccgat tgagctggtc tatatccagc
    29161 atgagaaaac cagcctatct aagatgggcg aacgctttat cgactatcta ctagaagaag
    29221 ttcagtttga tagttgagaa atgataagaa ccaatatgta ggctagcaac aacctgcaca
    29281 ttggttcttt ttacttataa ttaaaagttt cccctgccaa cttatcagct agcttgggaa
    29341 agagagtata aaacttatgg gctaggttca acaaaatcgg gagattgagt tctcgtttat
    29401 tttttcctat aatcttgaca atctttttag ccactgcatc tgcctctaag aggaagcggt
    29461 caaccgattt aagataagtt ccatctgggt cagcttggtc aaaaaatcct gttcggattg
    29521 gacctggatt gactgttgtc acatagactc catagggcat aagttcgagt cgcagagcat
    29581 ttgaaaaacc aatagccgca aacttggtcg ctgagtaaag actagacttg ccagtagcta
    29641 ttagacctgc catgctgacg atgttgatga tatgcccttt gctgctttcc ttcatacgag
    29701 ccgcaaggtg acgagacaga ttcatcaggg caaaggtatt gacctcaaac atctggtgaa
    29761 tatctttatc agcaatctgg tcaaatccct caaaaatccc gtaaccagcg ttgttaatca
    29821 agacatcaat cttgccatag cggagataaa gatcagttac cagagcttct agggctgaat
    29881 cgtcggtaat atcaatttca atcaattctg catgggaata atttccgtag agttgggcta
    29941 atttttcctt atttctacca agcaagatga gttggtcatt gggcaggagt ttgaccattt
    30001 cttgagctag accaccgcta gctccggtaa tgagaatagt aggcatactt atcctttctg
    30061 tgactgctag atttccactt cttccaagtc tttgaccaca tggacatttt caaaaattgt
    30121 ggcagcgtcc ttcttgagtt tgcttatatc ttttgagagg aaacgggcac tgatatggtt
    30181 gagtaggagg cgtttggcac ctgcttctac cgctacttgt gcagcttgca tattagttga
    30241 gtgaccatgg ttacgagcaa ttttttcatc acccttgcca taagtggact catgaactag
    30301 gacatctgca ttgacagcca gacgcacact ggcatccgtt tttcgagtgt ctcctaaaat
    30361 agtgataatc ttacctggac gtggcgctga gatatagtct gctgccttga tttcagttcc
    30421 gtcttccaaa acaagatcct ggccgttttt gattttacca aaaagcgggc cgaacggaac
    30481 accagcagcc ttgagttttt cagcatccag cgtcccttct agatcctttt gcatgacacg
    30541 atagccaaca cagaaaatag tgtggtccag ctcctctgca tacacagtga atttatcggt
    30601 ttcaagaatt ttacccagag aatcttggtc aaactcatgg aaatgaatgc ggtagggcag
    30661 acgagaacct gacacacgaa ggctggttaa gacaaatgac ttgattcctt gaggtccgta
    30721 gatttccaaa tctgtctgct cttcattggc ctgaaaggca cggctagaaa ggaaacctgg
    30781 caaaccaaaa atgtggtctc catgcagatg agtaataaag attttgctga ccttacgtgg
    30841 tcgaattgtg gtttccagaa tgcgattttg cgtaccttct ccacagtcaa agagccaaac
    30901 ttcgttaatc tcgtccaaaa gtttcagggc gagacttgaa acgttgcggg ctttagaggg
    30961 ctgaccagcc cccgttccta aaaattgaat atccattcga tactttctaa ttaatcaata
    31021 tataacatgg ctgtgcggtt ttccgatcgg aaatagcgtt tgccagaaaa agcagcagct
    31081 tcttgcaata aatcctcttg gctgtagcct ttgagacgtt ttcgaccatc agccaatctt
    31141 tccaaatcag tcaaagctgt gagactttct aggctgacaa cttcctcgtc cttgacaggc
    31201 ttcatgtaaa tcttaccaga ctcttcaaag actaattgat gggagaaaat ttgcgcaatt
    31261 tcaaagagca agtcatccga gattttctcc tcattttcag agaaaatccg accaaagccg
    31321 tcactctcat aacaaaaacc aaaggattta ccagacagat taagccgaat aaaaggctta
    31381 ttttctaggg tgaaacttgg ctcagtattg taaagattca gttcctgact gagttctgca
    31441 aaataatccg tcgcagcctg aggacttttt ttctgataga gttctgcaaa gtaggcatta
    31501 acaacacttg gcggaggtgt aataagtgtt aactgctcct gctctgtttt accagctaga
    31561 agctgatcca gatagacctt gtccagactt gtataacctc catactttag agccaaagtt
    31621 ttaatatcag tcataaaatt cttctaacct ccatttattt ttctcggaaa tgtagcctgt
    31681 aatcacttcg ccgtcttcct gataatcacg ttcttccaga attgcaacac tctctaaatc
    31741 atgaatcttg taggactttg aaaaaggcac tcgcagggta aatgcttcaa aaatttcctt
    31801 aatcttatct agcaataatg cttgcaagtt ttcacgactg tcctcagact tggcagaaat
    31861 gagggtatat ggcgtttggg taggcgtgaa atcctccacc aaatccgctt tattataaag
    31921 cgtcaagtga ggaatatctt ccatgtccag gtctttcatg atggagagaa ccgttttttc
    31981 atgctcctcg tggtaaggat tgctagcatc gataacatga accagaaggt ccacatgctt
    32041 gctttcttcc aaggttgact tgaaactgga caccaactct gtcggcaaat cttggataaa
    32101 gccaacagta tctgtcaaag ttacttggag attgcctccc agatgaatac tcttggttgt
    32161 cgcatccaga gtcgcaaaga gctcatctgc ttcatactgg gtcttactgg tcaagatgtt
    32221 catgatagtt gatttcccag cattagtata accaatcaaa ccaatcttaa aagtgctaga
    32281 ctccaaacgt ttttctctga cagtcgcacg atttttctca accaccttaa gctggcgctc
    32341 gatatccgtg atttgattgc gaacgctacg acggttcagc tccagttggc tttcaccagg
    32401 accacgggaa ccaattcccc ctgcctgacg gctgagcata atcccctgac caaccaagcg
    32461 aggcaagagg tatttgagtt gggctaggtg gacttggagc ttcccttcat ggcttcgagc
    32521 ccgcatggca aagatatcca aaatcaactg catacggtca atgaccttaa caccgagaac
    32581 ttcctctaga ttgacattct gccttggggt cagacggttg ttgacgatga cagtagtgat
    32641 ttcttctgca tccaccataa gcgcaatctc ttccaactta ccagagccga cgaaggtctt
    32701 ggaatcatat ttttcacgtt tttgtctgta gctatctaca acgactgccc ctgccgtttt
    32761 cgctaaacta gccaattctt ccatggagag gtcaaaactg tccataccct gcaattccac
    32821 accaatcagc aggactcgct cctctttttt ctccgtttca atcatctaaa aactcctcta
    32881 tctggcttaa aatgcggtct tgtacaccag attctccaat ctgataaaag gtgacctgca
    32941 tgcgattacg gaaccaggtc agctgacgct tggcaaaacg acgggtcgcc tgtttaagac
    33001 tctcacgagc ttcctccaag gtctgctctc cacggaaata aggaaagagt tccttatagc
    33061 caattccttt agcagcctgt acattagggg aatggtcaaa cagccacttg gcctcatcca
    33121 aaagcccagc ctcaaacatc aaatccactc ggtggttgat acgctcataa agttgactac
    33181 gttcatcatc caagcagata atcagcggtt catacaaaat ctcttgattt tccaaatcct
    33241 gaccaaaatg ggcaatttct aaggcacgca tagcacgacg acgattaaac tggggaatct
    33301 caaggcctgc ttgctccacc aaatgggcta attcctcatc tgaatatggc tccaaactag
    33361 ctcgataagc taaaatctcc tcatgaggag tctccccacc taggtggtaa ccttctagca
    33421 agctctggat ataaagtcca gtcccaccgg cgataatggc tagcttgcca cggctgtgaa
    33481 tatcctcaat agtcatctta gcttctgaaa caaaatcaaa agccgagtaa gactcagtta
    33541 tctctctaac atcgattaaa tgatgaggaa cagctgcctg ctcttctgga ctagccttgg
    33601 ccgtcccaat atcaagtcct cgatagactt gctggctatc tccactaacc acttcgccat
    33661 taaagcactt tgcaacttca atagctagag ccgtctttcc aacagcagtc ggtccaacaa
    33721 tcacaattat ttttgttttc atcttttttc cttgaaaaat tcccattttt tcgttactat
    33781 tattataaca caaaaaggtc agtcagaaaa atgtgacctc ttgaggaggc tggctgattg
    33841 agaatataaa ggaggaagac tcatggctaa aggattcgct aaaggtcttg taacaggtgt
    33901 cgcaggaact gtcgctgccg ttgcaggtgc agtatacgca tttaaaaaga aagtaatcga
    33961 accagaagag caaaaagcag ctttcatcga agaaaaccgt aaaaaagcag ctcgtcgccg
    34021 cgtatcacgt taagaaacaa aagaagttgg gattattctc aacttctttt ttctattctt
    34081 ttaaatagct aggtcaaact tcaactgtgg cttaacaggg tcataatcca ccaactcaaa
    34141 atcttctgct ttgatatcaa agaaattagt cccatcagga acatttaaaa ccaagcgtgg
    34201 ttggcagttt gacggctccc gacggagcaa ttcctgagct tgttcaaatt gattatcata
    34261 gatatggagg ttgttgatga agtagaagaa cttcccaacc ttccagccaa aatgtttagc
    34321 aatcatcatc tgcaaagcca catactgcat agcgttgatg tggtgggcca ccagcatatc
    34381 attggagcgc tgggtcaagg tcgcatccag atagatttcc ccatcaacac gccgaacatc
    34441 aaacatggtc tgaaaggcgc acgggagcag cccatctgtt tcttcgaaag cttggtaatc
    34501 ccagagagaa ataatattgc ggcggttcca aggattggtt tccaactgtt tgagaagctt
    34561 attgataatg tcgtgtttct taacaacggc accatagcgc tcaccaatgg ttcccgtgtc
    34621 tccaacttcc cagtcattcc agtagtgaac attgtacttg tcattaagca cttctaagct
    34681 atttgactgg tcttggtaaa tccagagaac ttctttgatg gcggatttga ttgcaatggg
    34741 acgcaaggtt atgataggaa attctccttt agataagtcg tactcggaaa aggcaccagt
    34801 tacatacttg gaattagcaa cagtcccatc cttgtactta ggacgagcct gctctgagaa
    34861 aacaccttcc tttagaatgc gttcaatatt ctctttaaaa atcgtatctg cttttgtcat
    34921 ttactctcac ctcattcatt gtcctaacta gtatagcata aaaaataaaa gaggaacatt
    34981 actaactcta ggttagcatt ttcctctctc attatcttat tgcaatacaa gtgatgctgc
    35041 tccgataact ccagcgtcat ttcctagagt tgcaagagct aatttagtag atgtgcgtac
    35101 ttgtgggaaa gtattttcgt catagacttt ttgaacacct tgtagaagga attctcccgc
    35161 agctgacaca ccaccaccga tgacgattgt tgatgggttt aggattgaac caatgttagc
    35221 acaagcgatt cccaagtaac gtgagaagtt acggtaaaca atcaaagcaa ggtcgtctcc
    35281 ttcttttgcg agatcaaaga cagtcttagc agttacttct tctccgttat caatcaagcg
    35341 tttcaaggct gcatcgcctt cgtattcatc ggcatagcga cgagtcaagt tgacaattcc
    35401 tgttgcagaa gcgactgtct caagacagcc tttcttaccg caagtacatg agattggctg
    35461 gtcaaagtca acagtgatgt gacccaactc acctgctgca ccagcaacac cgtgaagcaa
    35521 tttgccttct gcgacgatac cgccaccaac accagtaccg agtgtcataa agacaacatc
    35581 tggttggtta tcaccagcac ccatccagcg ctcaccaaga gctgctacgt tggcatcatt
    35641 atcgatgaaa aatggaatgc ccaaagcttt ttcaatcttt tgtttgattg gttgaagggt
    35701 tttccagttc aagttgtagg caccgataac agtccctttg tcacggtcaa ccacacctgg
    35761 tgatcccatt ccaatgcctt ggaagtccgc tgctgccaat ccaagcaagt ccaaacgatg
    35821 ttgaatagac tcaatcatat catcaacgat atgacttccc tcatccaaaa tgttggtctt
    35881 gattgaccat tttccttgga tttctcctgc tgttgttaag attgcaaatt tgatagaagt
    35941 tccaccaagg tcaatcccaa taatcttttg actcattata ttctcctttt tcattgtgta
    36001 gtaattgaaa atgcttacag tactagtata tcaaaatcca ttcacttatg caaaggtttg
    36061 cgtaaatgaa ttataaggaa ttttttcgcc taccagtatc tgttaaccat ctaaatgatc
    36121 aatggcatat tgagctgcat catttgaaaa tttatcaaaa tcagaagtta attgtctata
    36181 catacgtttt tttgacatgt ggaataacga attataactt tttgcctttc ctaaagcagc
    36241 agaatattca ctattgctat cattactaga agaagctgta cttgcttgac cttccttcca
    36301 aactccgtca gcgccaacat aataacgtcc accatcaacc cattcattga cagccatttt
    36361 accatccgat tttagatagt aatttccttg ccatgtacta taagcataag aaccatctga
    36421 tgtcaaataa taccaagctt gataggtggc atcataaacc cattcacctt tagccatttt
    36481 accatctgat ttcaaatagt agtttccttg ccatgcattg cgagcatatg aaccatctga
    36541 tttcaagtaa taccatgctt ggtaagaaga atcataaacc cactcacctt gtgccatttt
    36601 accgtttgat ttaaggtaat aagctccttg ccatgcattt tttgcataag aaccatctga
    36661 tttcaaataa taccaagctt gataagaaga gtcataaatc cattcactct gtgccatttt
    36721 gccatcagct ttcaggtaat agtttccctg ccatgcattt ttcactagat ttccatttcc
    36781 atcaaaatag taccaagaac cgtcctgctt aacccaacca cttgctgtgg atgaagttgt
    36841 atcagtttga gttgtagccg cttgttttgc tttaaaagta cccttaggag catttttcaa
    36901 ttcgcaagcc acaccatcat gatcgcggtc taacttggca gaataaccag gttctccctc
    36961 atgaagatcc gaatatccat tagcccaagc ttccttacag ctcgaaaaat ggatgttttc
    37021 ttccgccaaa actggttgtg aaaacaaggc taaaattggc aacgtcacca gactcatttt
    37081 taaaaataga cgtttattca tttctttctc ccataattat gatatcgatt tcaatattat
    37141 tttatcaaac ttatttcagc aattcaagga aacttctggt cttttctaca aactcctttg
    37201 gttttgcctt attcaagaca tgagggccat caggaatgtt ctgaaactgc gaatctgtta
    37261 acagtctatg gagagctttc atagaactaa gattcggttt atctttgctg ccacaaatta
    37321 gtaaggttgg ataggagcaa gtccctgcta tatccgttaa atcaagtgtc ttcaattcct
    37381 cagaaactcc gaccataaga gtcttgtctg ctccctgttt ttcaaatact cttttgggaa
    37441 gtagtttaaa aatcagcaac tgaagataaa ataggatatt ccctgctaat ttaagcgggc
    37501 atcctgacag aatcaaagct cgaagatttg gtaaatcgta actggaaagt tctagtgtca
    37561 gggcagcacc taaggacaat ccaatcaaaa caaaaggttc tgtctcttga gctaggtgct
    37621 gataaactcg ctctttagct tgttgatagt tactaactcc agaaggaaat aactcgatag
    37681 cctcagaagg ataatctgtc agtagattcc gaacttcttt ccaagactct gctgactgcc
    37741 ctaacccatg caaaaatatc aatttcatct agttctcctc aaggcttagt tcatacaagc
    37801 ctctcactgc attacagccg taaatagctt ctgcttgggt taaatctgcc aaggtcaaga
    37861 ctttctcttc tacctgtcct gtttctagca aatactgacg gtaaattcct ggtaagattc
    37921 caagtcggat aggcggtgtg tagagttttc cagcgatttt cagaaccaaa tttcctatag
    37981 aggtttcaag cagttctcct gacttattgt ggtaaatctt ctcttgttct cctaggctca
    38041 aatgcggtcg gtgagtggtt ttaaagtagg taaaggattg attcaaagca gcttcctgaa
    38101 ggcagacttg ggcctgacaa aagcttgtac tgagaggggt taatacttga cgattgactt
    38161 ctatctctcc agatttgcta aggctgattc gcaagcggta atctcgatta gcttcacaat
    38221 cctgacactc ttcctcaatc ttgtgtccca agtcttctgc atcaaaagga aaagcaaaat
    38281 aacgactagc ttttctcagt ctttccagat gttgatcttc aaacagcagg ttcttctggc
    38341 tgatttcccc tgtcgtaatc agttggaagc gagcttgttt acgataaaga actgctgcct
    38401 tttgatgaac ctctcggtat tcagattccc atgtgctatc ccaagtaatc cctccgccaa
    38461 ctccataaat ggcttgacct ttgtgaagtt gaatggtacg aatggccaca ttaaaaatcc
    38521 gtcgtccatt tggaagcaag agaccaatcg ttccacagta gactccacgc ggttgaggct
    38581 ccaagtcctt gataatctcc attgtcgcaa ttttcggtgc acccgttatg gaaccacaag
    38641 gaaagagtga gcggaagatt tcaacaaggt ccacatcctc tcgcaactga ctcttgatgg
    38701 tcgaagtcat ctgccaaaca gttgaatact gctctacctg acacagacgc tccacgtgct
    38761 cgctcccaac ttcagaaata cggttcatat cattgcgcaa gaggtccaca atcatcatat
    38821 tttcagagcg atttttggga tcctgttcca accaactggc ctgttcaaga tcttcttggt
    38881 cagttacccc acgctgagtc gtccccttca ttggtcgtgt tgtcaactcg cgatcatttt
    38941 gctcaaaaaa gagctctggg ctcatggaaa tcactgccat ctcgtcatgt tccacatagg
    39001 cattgtagcc cgcctcctgc tctaccacca tacgattgta gatggcaaaa ggattggcac
    39061 ttaacttttg cttgagttgg acggtgtagt tgacctgata ggtgtctccc tgcctcaagt
    39121 gatggtgtat ctgggcaatg gccttttcat agtctgctgc agacgtcact tcctgccaat
    39181 ttgagggcaa atcaatatcc tcataagtca gaggaatagg ggaagtttct acgctatcat
    39241 gaacagtaaa gtaaagcagg tactctccca gtaggggatc cttgtgaact gctaattttt
    39301 cctcaaaagc aggtgcagcc tcgtagctga cataccccac cacataataa ccttgctctt
    39361 ggtagctttc cacttgtgcc agcaaatctg ccacttcttc tacatttctc gttttcaact
    39421 ctttaatagg ctgggcaaag gtatatctct cccctaaagt cctaaaatca atcactgttt
    39481 ttctatgcat accttaagta tagcataaaa aggcagatac agacaaagct gatctacctt
    39541 tttacgattt tattcattct atgcttgtgg tgcagtaaat agatcaccag agaaaccgtc
    39601 tgtgttttta aggagagctt taaagacttt cgatttcaag acaactgtat ttcggtattt
    39661 gtctttaata ctactatcgt ttagttccgt taaatcttta gtaacagcct catcaaacat
    39721 agtcttcaac tgttcatagg ttgaaatttg agtgccatta atttcaattg gtgcaaaacc
    39781 ttttttagat tttgtaagaa cttccccata ccattgcttc ttccaatctt caaggtttga
    39841 aaattggtta ttagaaactt tcttaataat gaaatcatca cctaatagac cgttattttc
    39901 tttattggct gcaccttgat gtttgcttga aacatagtca atgaacccat gttcatatcc
    39961 atagtaaccc cacatacgga aggcgttatg tttgaaggat agagaaccag gggcaccttg
    40021 actcgtatta ccgccataaa taccagccat catattaaca ttgacatatg ccgtaccaaa
    40081 gtcctctggg tggaagacct tattacctgg atttccgtga atggtcatga aattattatt
    40141 taccaagtca tcgatggttt caaggttagt tgttttctca gcgtctatca aatcacgcac
    40201 tttgtcccat tggtgaggac cacttagttt atttctgtcc attggtcgac gtgtttcgcg
    40261 atcaattttc ttgaaccact tactattgtc acctttaacc ttaggaagaa cagcctcagc
    40321 ttctgcataa tcgagcatca tcatagcttc attgtagttc ttcatgtaat ggtcgatttg
    40381 gtcacgtgtt tgaagtttgg taggatctgg attataccat tggtttccat catttgaacg
    40441 ttcaaatgcc atatttaatc caagtgcgcc atattcacct tgatgtccat gttctgctgg
    40501 agtttgtaac ataccttgag caaatgcttc aagatctgta ccctgtcgat gtctatgacc
    40561 tcccagatat gccattcggt catttacgtg ggtagtctca tgtgtaaagg cagatgtgcc
    40621 atattcactt attaaatccg acataacaaa atatacagca tccataggct gtggatcagc
    40681 aaagatgtaa gcatacgctc catacccata cttataatca tgatacttat caatacgtcc
    40741 aaagaattca cgaataggtg cataatccgg tttatctttt cgatgaccaa atctatcagg
    40801 ccaaccaccg ggtgcaccat gattatccca tacaggagtt ggaactacat tttgactctt
    40861 aagaagttta tcgcggacat tatcagtggc aaggcgtgac caaaaatcta agtaattaac
    40921 ttgctcttta gcacgtttat tgatttcact cttgaacgat tcacgttttt cttctgtatc
    40981 tttaccatac ttttcaaacg aactaaatga gatagtatta tatgttgaaa tcataaataa
    41041 atgtgcatct ttcaagttca gaagcgataa aatcatgcgt ccatgttgtc cgttattaag
    41101 tccttcatat agacgatatt ttttattagc aaatgcaggg ttagttgacg gttgttcaac
    41161 tacataagca ttattttcaa tagctttctt aaaccaaaca tttatgtctg tttcatttgt
    41221 gaatagtttc atattgtagg tcaagaagtc atgtaagttt ccagaaagag tatttttcgc
    41281 caccacttca cggaaggact cctgtgtacg acttccctta atatagtcct cttttgaacc
    41341 aatttctatt aatcgatcta aaacacttac attcttacca taaaaatctg gtttgaatag
    41401 cattaattct ttaatattga agttatcaaa tttcacaccg taataacgat ttaggtatgt
    41461 taatcctaac aggacagcag ctttgttatc ttcaattttc ttaatcaagg cacgttttgc
    41521 agcttcatct gtattcagtt gatgatcttc attttcaact aattttgtca caaatgtatt
    41581 aagatgatct tttacttctt taaagctttc ttctaagtaa agattcttaa ttgcattaac
    41641 tctgttatcc ccagttcttc ctaatcgttg ataaattgga tcagattgta attcaattgg
    41701 acttaaaata ccttcaatag cactaattaa atctgcacga tctttaacaa ccatatttgg
    41761 tgtatagacg acttcaccaa gctctgcgac actgtattct tttacttgct taacttgaga
    41821 gtctttagga gaaatcgtaa agatatcttt tgtcttatct gcgtaatgaa ccataatatg
    41881 gtcagcatct gtaaggtctg taataaagtc attacctttc attgcagtca cagacaatac
    41941 ttctttattc attaatggac tatcagttgt aagcttgttc ccttgattca caatccattc
    42001 tttgttgtag aacggttgga gtttttcaac attacgataa acaagctcac gatttgcatt
    42061 ataatcttga attgttttgt attcttcatc acgtaatata cggcgattca acttatcaac
    42121 caaatgctca gttacttcaa atgtatcagc agtgataccc atatccttaa ttttcttatc
    42181 agcttctacc tgtgaaattt ctttaatttt agacgattgt ttatgggttt tatctccaga
    42241 actcacaccg ataactgttg cattattagg tttaatccaa ttgcttacaa attcatcatc
    42301 catatcatta gaaccaaaga aaatatcacc acgcttaaca ttcactaagc tgatattgtt
    42361 atcaatggtt ccccaaggcc agtctttacc aattaaaccg cctacatcac ctctagcaac
    42421 atcaatagta cctttggcta cagaatttct tacattagac caagcaccaa catctcttgg
    42481 attaaaatca tgagctaaat gtcctactat agcacctgca cgagtattta aacctttaag
    42541 ttcaacatct gcataagctt tatttaaatt gcctcgataa atctcagatg caatcccagc
    42601 caagttccac cctttatcac caacagattg aacttttccg ataaaagcaa cattagtcat
    42661 aatacctgca tcatctaatt tatccaaaat acccgcaaca ttgttattac caacgatagt
    42721 acccgttact ttaatatttt caacagttgc atttttcact gtttgagcta atgaagctaa
    42781 ttgatttgcc caaggcatat taatattaac gttaattaaa ttaatatttt tgatagaacc
    42841 cccttcaact ctatcaaata atggacgagc agtgttatga atggagaaat gcttaccatc
    42901 aacacttgtt aagtgacctg taaatttacc tgttacatat gatttacttg gtgtaggtac
    42961 atttgttgca tttaaatccg atcctaattt aaactcaccg ttaggatttt tctgcatagc
    43021 atctactaaa tctttgaagt tatagtaaac gttattttgc gatgctttgg gtttctctat
    43081 gtaataagtg tatgtttgac tcaaactatt atttgaatca cgttggatta aatcaggagc
    43141 tgaagcagta actttaaata aggatgtctc ctcctcagtt acttcctcta tattagtaat
    43201 ggcaagcctt gttactttat tatgattagt ggtaattttt agataatatg acgttagatt
    43261 ttctggaaca gtttctagaa ggctactatc tgtttcattt ccattctcat ccactttcat
    43321 caatcttgtt tctttaatat ctttaatctc aactttttta aggtctaact gaacttcttt
    43381 atcttctaat atttcagttt cttcaccgtt acctcgatca tagaccatgg ttgtggatag
    43441 tgtataacct tgataatatt tcaagttgtc taaagtagct tgtaagttat tttcttctac
    43501 cgttacttct ttaacaactg ttccagtatt atcttttaga gctactttaa tcgatttaat
    43561 ttctactcca gctggtttag tcaatgtata actagctgtc gcactcttct tcaagacatc
    43621 tttatctgtt ttagcccatt ccaaggttgg tttctctaac tcttttgtgc cattcttaat
    43681 aattttgtct tgaggttttt caatcactgt ttctgtgata gtaggagcgt cacttgtttt
    43741 ttctccgcga attgttttat aggtttcagt aatttgtttc ttatcgttta caccttcttg
    43801 agttacttgt cttgttccgc gttttaaagt gtcatcttct tgttcttttg tttcaaaagg
    43861 aatgatttct tctcttgtct caaccgttgt cccttcaatt ggttttgtac cacggattac
    43921 ctgttgattt acaggctcct tggtgacttc cgtactggtt gaaagaactt ggtcagtttt
    43981 aacccctcct attgttttat aggttgtttt tgtaacttga cttccttttt caccatttcg
    44041 taggacagtt tcttcgtccg tgtacttggt tgggtcctca gtcgtttcag ttttatagtc
    44101 aagttctgta gttgattctt caacgagtgt tccttcagtc accttatact caggtaattg
    44161 ttctggaaca agagcctcac ccacgtaatc ttcttcttgc gtccccttag ctgttttagt
    44221 ctcctctttt ggttctacag tttcttcagc cttggtatct ggagttgact cttcttgttt
    44281 cggtgtctct tccgctcccc tatcttcttt aggattgtct tcttgtgatg attcttttgg
    44341 tgtacttggt tgctcagctt gtgtaggttg cacgggttgt tctgggactt ttgcttgttc
    44401 tactggtggt tcaacttttg attcctcagc tggtttgtct gatggttgac tttctggctt
    44461 aactgctact ttttcctctg attttgactc aacttctcca cctacttctt caactggagc
    44521 tggttctgct gaatcttctt tcccctcttc tactttagga agggtgtcgt cagtaggttt
    44581 tacctccgat tttggttctt cctttggact ttcttctgtt ctaggtgctt cttcttttgg
    44641 agtttcctct gtctctacta cttggttttc tgtcctagct tgctcctgat ttgttgttga
    44701 ttgaggagtc tcaacttcga ctacagtcac ctctccaggt tttgctgagg tttcttctaa
    44761 aacagtgtcc aagccaagcg ttttgaggat gtcacctgat agataaccaa catagcgata
    44821 gccatccatt tcaacaacac cctctcgact agccagcgct aaggtcgcaa ctgggtctac
    44881 agcccctgca ctaggaagaa ctaccaatcc catagctcca actagaaaga cgctagcaat
    44941 tttctttctc ttgtagatta aaagcaagct cccaacagtc agcaaaccaa aagctgtcaa
    45001 aacagatgct tctgtccctg tttgaggcaa ctgatctttt tgatacacca aaccatatac
    45061 aacttcattc ctgtcaggct ttcctgtctg aattaaatct ttagcttctt gtgaaataat
    45121 ctctttattt acatagtgat aggtagctgc gtccactaca gaaggagcca tcaaaaggct
    45181 tccaagaaat acagagccta caactccctt aatcttacga attgaaaaac ggtctttttt
    45241 aaacactttc atctccttta ttcattctca agactttcta atagcatctt gcggatagtg
    45301 cgcacgcgca cctccgatta attttggacg actagccagt gccgttacat gggcatgacc
    45361 aatctctctc aaaatagggc gaatcggaac ctgaacatgc ttgacatgca tgccaattgc
    45421 agtgtctccg atatccaatc cagcatgagc cttgataaat tcaacctcaa ctggatcctg
    45481 cataaactta aaggctgcca actgccccga acctcctgca tgaagagtag gatggacact
    45541 gacaatttcc agatcaaact gctctgccac ctgacgttca acaacgagag cccgattgac
    45601 atgctcacaa ccttgaactg ctaaatggat acctctacta cctagaatac ccaagatagt
    45661 ctccactatc agctcaccaa tctcttgact ggattctttc ccaatctgac cacctagcac
    45721 ctcactagaa gatagaccta aaacaaaaag gaccccctgc ttcaaattgg tcttttctaa
    45781 aacatcttcc actacctgac gtgtttctct ttgaatctgt gtctcgttca tctctgttac
    45841 ctctgttgtc actcttctat cataccgttt tttcttgttt ttagcaagat agacaaccta
    45901 taaagtctgc ccaattacgc ataaaactcc cagaattgac tgggagttag ctagtttcta
    45961 ttctatttat atatatttca actttcgttc ctttttgggg tctagaatca atcttcatct
    46021 ggtaattggc tccaaaatga agtttgagcc gttgatcgac attttgaaga ccaactcccc
    46081 cacgtttgag ttgactttga ctactatcac cagcatcttg gaagccaacg ccatcatcct
    46141 caatacggat gaccaatccc gaatcctgtt tctggacaga aagtttaata tggccctgac
    46201 cttccttttc cttaatgcca tggtaaagag cattttctac aaggggttgt aggaccagct
    46261 tgggtaagac taaattatca aaggcaacat tttcattaat ttcgtattcc agcttatctc
    46321 catagcgttg tttctggata aagagatact ggcggacatg attgatttcg tcagagagac
    46381 aaatcaagtc cttgccttga ttgagcgcca agcggaaata ggttgccaag gacttggtca
    46441 cctgcaccac tcgctgacta tcatgaaatt cagccatcca gatgatggtg tccaaagtgt
    46501 tatagaggaa atgtggatta atctggctcg aaagggcttg aagttggtac tgacgggtcg
    46561 tttcttcctg gctacgaata gctaccatca actgatcaat ctgatccaac atagcattaa
    46621 attggcgagt tacttctctc agttcatagg caccaacttc cttggcacga agattttgag
    46681 caccagaagc aatttccaac atggtttctc tcaaatcctt caaaggagca atccagcgtt
    46741 taagactgaa ccacactaag cagagacaaa caagaagaga tgtgacactg gccccaagca
    46801 aggtccacaa gagctgactc cgaacctggt ctaacttttc caatgatgac acgccaagta
    46861 ccgtccaatc agttcctgca atcttctctt gactgacgta ggatttgtga ccaggagtat
    46921 aaccctgacc tgtctcgatg tagggtttca tagcctccat tttgctagac gaactataaa
    46981 ctgtgtgttg aggatggtag acaaattcat ggttttcatt gataatgaag gcaaagccct
    47041 gctgccccaa ctggagttga ttgagatagg cttccagagt ttcataagaa atatccaaac
    47101 gaagcacacc aagattggct ccctttgcat caacaagttc ttgagtgaca gaaatgaccc
    47161 actgactatc tgatttacga gctggagtca aaacaggcat agctccctga tgaatagcct
    47221 tttggtacca atcctcagcc atcatatcag aggaagtttt catctgcaca ctgtcatctg
    47281 tagaaatgac ctgaccagat ttggtcacca gcacaacagt tttcaagtcc ttatctgact
    47341 tcaagatggt caaaaacaaa tctcggattc cctcgacctt gtcttgactg ggattctcag
    47401 cataggccag aacatccgtc tgctgggtca aaccagtcga ggtggtttct agttttttga
    47461 tataagactg aataaagtgg ctagtctggc tgatggtcgt ttggctgttg ccctcaatgg
    47521 tggcctcaat ggctgaagaa cttgattgat agtagaaagt tccaaccaga gctaggagaa
    47581 tgagaaagac cagaaagatg gaaataacca ttctaactaa aagagaagaa cgcttcatcg
    47641 gtcttctccc ttcttaaact gacgaggtgt cacacctgca atctgcttaa aacgttgggt
    47701 aaaatagttc atatcttcaa aaccaacctt ctctgcgatc tcataaatct tcagatctgt
    47761 agttaaaagc aagagcttgg cttgtttaac acgttctctc accagataat cctgaaaagg
    47821 caagcccaac tctttcttaa tcaaggaact cagataggtc ggactaaaac ctaagtcact
    47881 ggctaaagac tttaaactaa attggctatc agccagatga gactggattt tctgggccat
    47941 gtttccttca aacctattag tcaataaatc ttgtaactgc tcttctttct cttccttgtc
    48001 tagtttttgt ttgattttcc ccaacatttc ctcaatatcc tgacgagaaa agggtttgag
    48061 caggtagtcg tccacaccta gtttgacagc agacaaggca taatcaaaat catcgtaacc
    48121 tgttaaaaag accaaatgaa cctgaggata ggtttctcgt accagactgg ccaactggat
    48181 gccatttaga tgaggcatgt tgatatcggt taaaatgata tctggcacct gcttttggat
    48241 caattcccaa gcctgccttc cattttcagc ctgaccgatg atttccatat cgtaggctgc
    48301 tacattgacc agtttagtca aaccttgtct taccagatat tcatcttcta cgattaagat
    48361 tgtgtaggtc atgctctgct cctttaccac ttactagtat cagtatagca aaattctcct
    48421 ctaactgctt aggaaagacc tcttatactc aataaaaatc aaaaagtaaa ctaggaagat
    48481 agccacaggt ttctcaaagt atagctttga ggttgtaaat aaaactgacg aagtcagatc
    48541 aaagtatagc tttgaggttg ta
//
LOCUS       DAVBCN010000006        47180 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVBCN010000006 DAVBCN010000000
VERSION     DAVBCN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 47180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47180
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.006"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(<1..533)
                     /locus_tag="VQ497_000319"
     CDS             complement(<1..533)
                     /locus_tag="VQ497_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516683.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIYEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLS"
     gene            complement(523..720)
                     /locus_tag="VQ497_000320"
     CDS             complement(523..720)
                     /locus_tag="VQ497_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516684.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(724..1308)
                     /locus_tag="VQ497_000321"
     CDS             complement(724..1308)
                     /locus_tag="VQ497_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516685.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEIPKELTPSEQLTWIAFLLPDGDIKQIKSVSNLLYGGAYALS
                     HSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLK"
     gene            complement(1716..2141)
                     /locus_tag="VQ497_000322"
     CDS             complement(1716..2141)
                     /locus_tag="VQ497_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516686.1"
                     /translation="MTSRVFIDADCISAFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNYDSNKIIGKGEATSIFLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(2134..2628)
                     /locus_tag="VQ497_000323"
     CDS             complement(2134..2628)
                     /locus_tag="VQ497_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516687.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMVLGHYIKSTEQLLENNRISQGKYEELLLDAFRYDIVYGLDEEGE
                     LSFD"
     gene            complement(2674..2880)
                     /locus_tag="VQ497_000324"
     CDS             complement(2674..2880)
                     /locus_tag="VQ497_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516688.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            complement(2867..3028)
                     /locus_tag="VQ497_000325"
     CDS             complement(2867..3028)
                     /locus_tag="VQ497_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2516689.1"
                     /translation="MNMINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            complement(3393..4349)
                     /gene="lytA"
                     /locus_tag="VQ497_000326"
     CDS             complement(3393..4349)
                     /gene="lytA"
                     /locus_tag="VQ497_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HET2516690.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPDFTVEPEGLITVK"
     gene            complement(4725..6095)
                     /locus_tag="VQ497_000327"
     CDS             complement(4725..6095)
                     /locus_tag="VQ497_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET2516691.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYHLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            complement(6402..7568)
                     /gene="recA"
                     /locus_tag="VQ497_000328"
     CDS             complement(6402..7568)
                     /gene="recA"
                     /locus_tag="VQ497_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET2516692.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
     gene            complement(7623..8879)
                     /locus_tag="VQ497_000329"
     CDS             complement(7623..8879)
                     /locus_tag="VQ497_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET2516693.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNLLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQVDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGISSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            complement(8963..9979)
                     /locus_tag="VQ497_000330"
     CDS             complement(8963..9979)
                     /locus_tag="VQ497_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET2516694.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEKALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            complement(9987..10505)
                     /locus_tag="VQ497_000331"
     CDS             complement(9987..10505)
                     /locus_tag="VQ497_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2516695.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            complement(10495..10938)
                     /gene="tsaE"
                     /locus_tag="VQ497_000332"
     CDS             complement(10495..10938)
                     /gene="tsaE"
                     /locus_tag="VQ497_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET2516696.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            complement(11024..11644)
                     /locus_tag="VQ497_000333"
     CDS             complement(11024..11644)
                     /locus_tag="VQ497_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516697.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRALVLVIVTLIFF"
     gene            complement(12027..12890)
                     /locus_tag="VQ497_000334"
                     /pseudo
     CDS             complement(12027..12890)
                     /locus_tag="VQ497_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            13055..13249
                     /locus_tag="VQ497_000335"
     CDS             13055..13249
                     /locus_tag="VQ497_000335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516698.1"
                     /translation="MKKKRVSRMKKNRGIQKLAILVLLGVFMFSNTIPYQQFIQKNRQ
                     LEIRVQSQKKSNGLDVGKAD"
     gene            13564..13860
                     /locus_tag="VQ497_000336"
     CDS             13564..13860
                     /locus_tag="VQ497_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET2516699.1"
                     /translation="MLKFRKVVASSLAVLAFSLALPVLAVSHRGGEWTYGGHHDPNNW
                     GAFSNYYHGSQYHWAYVGSNGRNNQKTVYAGARSAAYAFVNTNFGEQVTFDAGW"
     gene            13963..15978
                     /locus_tag="VQ497_000337"
     CDS             13963..15978
                     /locus_tag="VQ497_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2516700.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSYYYPNVVVHDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKKLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMNLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFIALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAGLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            15980..16612
                     /locus_tag="VQ497_000338"
     CDS             15980..16612
                     /locus_tag="VQ497_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2516701.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLTYLDLDKRIFELSGGESQRVALAKVILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDRLK"
     gene            16753..17031
                     /locus_tag="VQ497_000339"
     CDS             16753..17031
                     /locus_tag="VQ497_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HET2516702.1"
                     /translation="MKKFDNYIVEKPCDSNSDKLQKILIIENLVDDILQFPLRINNIV
                     GEIFLLQPFQKKTIFIPCYFEEDIVKVKDDDKVEWNLLEFQKFRAFLA"
     gene            complement(17540..17953)
                     /gene="ndk"
                     /locus_tag="VQ497_000340"
     CDS             complement(17540..17953)
                     /gene="ndk"
                     /locus_tag="VQ497_000340"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET2516703.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(18064..21741)
                     /gene="rpoC"
                     /locus_tag="VQ497_000341"
     CDS             complement(18064..21741)
                     /gene="rpoC"
                     /locus_tag="VQ497_000341"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET2516704.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKTEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGEVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPYAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(21774..25385)
                     /gene="rpoB"
                     /locus_tag="VQ497_000342"
     CDS             complement(21774..25385)
                     /gene="rpoB"
                     /locus_tag="VQ497_000342"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET2516705.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLKEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            25800..25870
                     /locus_tag="VQ497_000343"
     tRNA            25800..25870
                     /locus_tag="VQ497_000343"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25832..25834,aa:Cys,seq:gca)
     gene            complement(26068..27399)
                     /locus_tag="VQ497_000344"
     CDS             complement(26068..27399)
                     /locus_tag="VQ497_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HET2516706.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFNVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(27690..28514)
                     /gene="endA"
                     /locus_tag="VQ497_000345"
     CDS             complement(27690..28514)
                     /gene="endA"
                     /locus_tag="VQ497_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HET2516707.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSTANSPKTKLSQKKQ
                     APEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(28553..28741)
                     /locus_tag="VQ497_000346"
     CDS             complement(28553..28741)
                     /locus_tag="VQ497_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET2516708.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDSW
                     AILSPAKWQELIHKFTGN"
     gene            complement(28734..30017)
                     /gene="murA"
                     /locus_tag="VQ497_000347"
     CDS             complement(28734..30017)
                     /gene="murA"
                     /locus_tag="VQ497_000347"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET2516709.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(30041..30271)
                     /locus_tag="VQ497_000348"
     CDS             complement(30041..30271)
                     /locus_tag="VQ497_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HET2516710.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTAENTRKVRV
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(30329..31366)
                     /locus_tag="VQ497_000349"
     CDS             complement(30329..31366)
                     /locus_tag="VQ497_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET2516711.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(31350..31838)
                     /gene="coaD"
                     /locus_tag="VQ497_000350"
     CDS             complement(31350..31838)
                     /gene="coaD"
                     /locus_tag="VQ497_000350"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET2516712.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLCVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            complement(31828..32367)
                     /gene="rsmD"
                     /locus_tag="VQ497_000351"
     CDS             complement(31828..32367)
                     /gene="rsmD"
                     /locus_tag="VQ497_000351"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET2516713.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEVGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKTVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(32432..33424)
                     /locus_tag="VQ497_000352"
     CDS             complement(32432..33424)
                     /locus_tag="VQ497_000352"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HET2516714.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(33536..34219)
                     /locus_tag="VQ497_000353"
     CDS             complement(33536..34219)
                     /locus_tag="VQ497_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HET2516715.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(34275..35015)
                     /locus_tag="VQ497_000354"
     CDS             complement(34275..35015)
                     /locus_tag="VQ497_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET2516716.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRNQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            35049..35327
                     /locus_tag="VQ497_000355"
     CDS             35049..35327
                     /locus_tag="VQ497_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET2516717.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            35405..36331
                     /gene="yidC"
                     /locus_tag="VQ497_000356"
     CDS             35405..36331
                     /gene="yidC"
                     /locus_tag="VQ497_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET2516718.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(36406..37200)
                     /gene="pflA"
                     /locus_tag="VQ497_000357"
     CDS             complement(36406..37200)
                     /gene="pflA"
                     /locus_tag="VQ497_000357"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HET2516719.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(37326..38576)
                     /locus_tag="VQ497_000358"
     CDS             complement(37326..38576)
                     /locus_tag="VQ497_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET2516720.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(38643..39470)
                     /gene="purR"
                     /locus_tag="VQ497_000359"
     CDS             complement(38643..39470)
                     /gene="purR"
                     /locus_tag="VQ497_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HET2516721.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLHEFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(39737..40678)
                     /locus_tag="VQ497_000360"
     CDS             complement(39737..40678)
                     /locus_tag="VQ497_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HET2516722.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            complement(40668..41924)
                     /locus_tag="VQ497_000361"
     CDS             complement(40668..41924)
                     /locus_tag="VQ497_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET2516723.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(41926..42588)
                     /locus_tag="VQ497_000362"
     CDS             complement(41926..42588)
                     /locus_tag="VQ497_000362"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HET2516724.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDGFVGVDRGSLWILE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFKQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLASYMHQIEIEDGQNLITYCPEGISQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(42551..43207)
                     /locus_tag="VQ497_000363"
     CDS             complement(42551..43207)
                     /locus_tag="VQ497_000363"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET2516725.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDGHFVP
                     QISFGAGVVEALRPHSKMVFDCHLMVSNPEHHLEDFVRAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALRDEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            complement(43218..44096)
                     /gene="rsgA"
                     /locus_tag="VQ497_000364"
     CDS             complement(43218..44096)
                     /gene="rsgA"
                     /locus_tag="VQ497_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET2516726.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDGGELDFYQQTYGDIGYDFVTSKEELLPLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     TAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGTIAPFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(44689..45462)
                     /locus_tag="VQ497_000365"
     CDS             complement(44689..45462)
                     /locus_tag="VQ497_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HET2516727.1"
                     /translation="MRKKLVNSLMCAVVLSGFPIATYANEKNIGPNITVSNGQTRLSI
                     PEKNGVEVIQSEENRVVYEVKEAAKIETSDGDMVAPIVVDSRITSINNQVVQGKTVYT
                     ADLEKATFVEEGYNKLAKPTNILDHIFGIQKVHAYTQNITTHQSQFDSTYSVELYTVV
                     HWVSYDGGVQKNITKISGGVDFHERDIEIESSSVEVRQGGWYSSQQKWFHPGARSSWE
                     YSTGFSKVGPGENRVVTNYTVNLIRYGDRWSAYLQNNPW"
     gene            complement(46152..47024)
                     /gene="rsmA"
                     /locus_tag="VQ497_000366"
     CDS             complement(46152..47024)
                     /gene="rsmA"
                     /locus_tag="VQ497_000366"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET2516728.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAEVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPVPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLE
                     EFASLADALKGQGL"
BASE COUNT        13847 a        10447 c         8732 g        14154 t
ORIGIN      
        1 ctgctcaaaa cactgttttg aggttgtaga tggaagctga cgtggtttga agagattttc
       61 gaagagtata aaccacaatt actcaatacc gaatacccct gaatggtttc tttttcatgc
      121 accaatatat tataataatc ttcatccaat aataaggctg ataaactagc atcttggtca
      181 aaatgtaagg gaatttctcg accgtccttc tttaatggat attctggtaa gatactaaat
      241 agttcaatca tagaaggaaa ctcaggatta gttgttgtaa atcgaaaaag ctgcgctttc
      301 tcatctttct gacttccttg atactctccc aattctaaaa aatgattcaa ggtagtataa
      361 aattccttat tttttacttc ataaatgatg accatatcat aatcttttgt tgtgcgactt
      421 ttaaatcctt gcgaatccaa tacgatagag gtagcagttc ccccaatcag aacataatag
      481 ttctgaaaat ccgcaaacat ttcttgaaaa ataactttcg tattagctgg catcatcttc
      541 tcccagatac tgtaatatca tattttctag tgcttcactc tcttcctcta tacgtgggtc
      601 atcatcatct tttaaggtca aataaagaga aatcggatct acaaattgtt tatcatgatt
      661 atttttaaaa tcattccaaa actcagatac aaaaggacga tatttccata tctctagcat
      721 ctttcatttt aaaacatgct gagaaagtgg caaggataac tgattgaatt ttctctgcca
      781 tatgacatag ctagtatttt catccgtttc agctaaaaaa gttgaatgcg acaaagcata
      841 agcaccacca tatagaaggt tagaaacaga ttttatctgc tttatatcgc catctggcaa
      901 taaaaaggca atccacgtta attgttcgct aggtgttaat tccttaggga tttcagtatc
      961 attcgcattg agtactagtc ccaatggagg gaagaagagg tttcccttaa agtctacaaa
     1021 cggaactcta gcttgaagta attgcttttt ttcactgtct gataacttcg aaaacaccaa
     1081 aacaacatcc atattggctt tttcacccat agtgcgagcc tgagtaacaa atgaactcaa
     1141 actccccctt ctcttttctt taatcaataa aaatgattcc ttatgaaaag tcatttgagt
     1201 atactcaaac ctttttacaa aactgattgg taaagtcaaa gtcagttcct caatactgac
     1261 atcaacataa aaggattgaa aaattgacat tatctttttg acattcatgt tttttcctta
     1321 ttctatcatt tttattatat tttatcattc ggtataataa aatataagtt ttttgataaa
     1381 ataaccaaaa actactcgct atcccctctc acattaccct acatatcgtt tgacatgcga
     1441 ctgatagttc aggaaaactt caaggagaac ttttctctca tccactatgc aggacttact
     1501 atttcacttc tactcctata ggctcaattt gacacttttc tttttcgaat tgctcatatg
     1561 cctcctgaat agtgagttcg atttatccaa atttattttt gtctattttg atgaatactt
     1621 ccacgaagat agtctgaaaa tgaatttgca ccaattttcc ttctcttttt aagcatatta
     1681 ttccagatat gattgccctc ttgttcagta ataaattacg ctttaaacgc ttcaatcagt
     1741 atatctcctg ttgtcatatg ttctaaagaa aattcttcta catatggttt aacatctctt
     1801 aggttattac ttcctaatat cccattatgc tttttcgcta agaaaataga agtcgcttct
     1861 cccttaccaa taatcttgtt actatcataa tttcttgtta aatctctata taatgcgtat
     1921 tcttcagttc caatgtctat gctcacaatc tcagctgaac ccttagttac caactggtct
     1981 atcctagatt ttaaatgggg aattgtaggt atattgattt catcatacac ctcttgtgga
     2041 ataacaattt tacccgaata gagcttttct aaaagatgtt cagtgccaac ccataaaaat
     2101 gctgaaatac aatctgcatc aataaataca cgactagtca aacgacaact ccccctcttc
     2161 atctagccca tatacaatat catatctgaa agcatctagt aacagttcct catacttccc
     2221 ttgcgaaatt ctgttatttt ctaaaagttg ttcagtcgat ttaatatagt gccctaatac
     2281 catttccttt ttactttctg acaaaggacg atataaactt gtatcatagc ctaatcttga
     2341 agctgtctct ataacactaa tatccatatt tttaatttct tctgcatcaa ggtatccatc
     2401 atttctcaat ctatataaca tagctttatg actgatacca taaaactgac ccaattttat
     2461 aatatcttct acttcaagat gagttctatt ggcattttct ctgatttcct caaccatcct
     2521 atacagtgaa gatgggaaaa ttaaaaaata agaagcaaac tgatccgctt ttctttcagt
     2581 ttcatctcct tcaccaatca agataagact gactgaactc ttcttcacct catcataata
     2641 aagatgatac agtccatgtg ctaaagaaaa tctttacctt cctaatggca tgtctgaatt
     2701 gactgcaatg agactgaact gagttccttt ataacagact ccgctaatat tctttccgag
     2761 tccataaaat accagcgtca aattttctat cttttgtacc aatttaaaaa tatctatcgg
     2821 cgattcacca tcagctccca acttttttct aagatttgaa gctttattac ttaaatccat
     2881 tcgtgatatt cctttcacct ttttccattt tggcaatcat gctttgattc aaagacaaat
     2941 actcagcaat cttatcttga gtaagatttg aagatattct aagttctttt attctttttc
     3001 caacatcaca tacatttatc atattcatac acctctcata aaaagaatag cacattcttg
     3061 tcataatttt ttaaataaaa aaattatgac aaaacaagga agcaatttat tgatgctact
     3121 taaaaatcta aaattgatga tattaaacct atttgatgag ttcctatcaa aaatcgtatc
     3181 ttcaacctca aaacagtact taaagctatc cgactcggtt tacattgtca aatttagatt
     3241 ttatttgagc ataactttct agtttgcttt ttgatttttg tttaatatag taacaaaaat
     3301 tgaaggaaat atctccacaa gaaaacgcat actattaagc tttttcaaga cctaataata
     3361 tgcgctgttc tgatttgaaa gacattccat tattatttta ctgtaatcaa gccttcaggc
     3421 tctactgtga agtctggctt gtctgccagt gttccgtctg gtttgaggta gtaccagcct
     3481 gttccgtccg ctgactggat aaaggcattt gataccatgg cgccttcttt agcgtctaag
     3541 tagtaccaag tgtccttgta cttgacccag cctgtcttca tggcaccttc ttcgttgaaa
     3601 tagtaccact tatcagcgat tttcttccag cctgtagcca tttcgcctga gttgtcgaac
     3661 cagtaccagt tgccgtctgt gtgcttcctc cagcggtctg caagcatata gcctgaactg
     3721 tcaaagtagt accaagtgcc attgattttc tcaaacttgt cttttggata agagccgtct
     3781 gaatgtacgt accagtagcc agtgtcattc ttctgccagc ctgtttcaat cgtcaagccg
     3841 ttctcaatat catgcttaaa ctgctcacgg ctaatgcccc atttagcaag atatggataa
     3901 gggtcaacgt ggtctgagtg gttgtttggt tggttattcg tgcaatactc gtgcgtttta
     3961 attccagcta aactccctgt atcaagcgtt ttcggcaaac ctgcttcatc tgctagattg
     4021 cgtaagagtt cgatataaag gcggtagtcc gtcatgaact cttctttggt tgaatggctt
     4081 tcaatcagtt caaccgctgc ataggtctca gcattccaac cgcccccaac gtcccaggca
     4141 ccattatcaa caggtcctac ctgcatgatg caaccgttcc caacaatgtg cgagaaaaaa
     4201 cctaattctg ggtctttccg ccagtgataa tccgcttcat tctgtacggt tgaatgcgga
     4261 ttcccagttg agtgtgcgtg tacttgccta tatggttgca cgccgacttg aggcaaatct
     4321 gttcttaatt tactcacatt aatttccata ttctactcct tatcaattaa aacaactcat
     4381 tttttacaat ccagaaccag aaactccttt atttctacct tacaaagaag acaatcttag
     4441 tcacgattaa gcttgtagat agaacctcaa aacgcactat tttgacactg taaataggac
     4501 tgacaaggtc tgcattctat ctacaataac accccagact aaaaagcttt tcaaaagtat
     4561 atttttacag tctctatatg tccttttcat aaatactata ctttattata tcacataaaa
     4621 gaagtcaaaa gtctgttaaa ctattttcaa caccaaacta aagaagagaa cacaagtttt
     4681 ttcgatgttc actagaggaa atggatttta ttcagtaaat ccaactagga ttgcactttg
     4741 gttgccaaaa ttgcctttcc ttcttttatc aagggatgac ggaatagtga gaagtacagt
     4801 tgaactgtca tggcaaccca gataagaggt tcacaaagga taacaccctt atatcctgcc
     4861 caaggaataa tcaaaaccac aaaaacgatt tttccgatta gttcaataaa gctagaaact
     4921 agaggaagga tcttctgccc caagccctgc aagcaattgc gataaatcaa caagaggctc
     4981 aaaatgggat aaaaggctga actgatttgc agatagagac ttccattttc tatcaagtaa
     5041 ccatctgtcg aactagccaa gaaggaaacc aaggctggac tggcaaaaaa gaggaaaata
     5101 caaacaaaaa ctgcccagga tatacttaaa cgactgccga ttcgaagacc ttgaacaatg
     5161 cggtcaggtc gcttagctcc tagattctga gaagcaaagg tcgtcattga tgcagaaata
     5221 gcggtcatag gaagaagggc aaaggtcata atgcgtcgag ctgccgtctg ggcactaata
     5281 atcactgcac caaatgtatt aacagaaaac tgtaaaatca cactgccgat agatacaatt
     5341 gaactcatca agcccatagc caaaccttgc tccaagagat ccgcgtacaa gcttttgtcc
     5401 catttgaaat gtttaaactg tggcaagagt tctggcacac ttttacgaat ataataaaag
     5461 cagagaaccg ctgataaacc ttgcgaaata atggtagcaa gtcctgcgga ttgaactccc
     5521 agatgcaatt gcgtaataaa atagagatcc agaaccacat taaccaaggc agagaaaatc
     5581 agaaatccca gggctgctag actgtcacca atagaccgca acaagcctgc aaaaagatta
     5641 taagcaaagc tgacacctac acaggtcaca atcatagaaa tatattgata agattgagga
     5701 agaatttctg caggagtatc taagtattgc aagagatgat acaagccaag aaagcccagc
     5761 aacataacta gaatgctcaa aagagcacct aaaatccaag tagctgctac tgcttccttg
     5821 attttagtga aattccgagc cccataataa cgagcaatga caatccccat gccattgcca
     5881 acaccaagtg taaaacctac aatcaggtca aaaatcgctg tcgtcgctcc tactgcagcc
     5941 aaggattctt gaccaagaaa tcgtccaaca atcaagacat cagcagtgtt atagagctgt
     6001 tgaaaaatat ttgatagcaa aattggaaag gtgaagctta agagcgaggg aagaatcgga
     6061 ccatgtatca ggtccactgt tcgtttttta ttcataaagc tattatatca gctttccaga
     6121 ggagcgacaa gaaacagcaa acttatatat agcggaatga aataaggaaa gtcaaatcga
     6181 tttttaacaa tgttttagaa gtagatatgt actattctag tttcgatcta ttatagttgg
     6241 ttgagtttgg aatagtacgc tgtagctgct aaaacatttc tagaaattaa tttgactttc
     6301 ctaatagagt tgttcatatc ttatttcaag ctactatata taatctaaaa acttgaatca
     6361 aaaatcgaaa aagtagcgga tttctccact gctttaacag cttattcttc aatttcgatt
     6421 tcaagttcat cgcctaagtc aagcggaact tcttcattca ctgcttcttc tttctttggc
     6481 tcatcttttt tgttttcagt atcttgttct gaaacttctt ctccatcaat caagccaaat
     6541 ttagaacgga cttgcttatc aatttcatca aagatttctg ggtgctctgc caagtatttc
     6601 ttagcattct cagaaccttg cccaattttt tcatctttgt aagaatacca agcccctgct
     6661 tttttgataa tatccaaatc gcttgcaatc ttcaaaagct caccagtctt agaaattcct
     6721 tctccgtaca taatttcaac tacggcttcc ttaaacggtg gagctacctt attttttaca
     6781 accttaatct tagtttcttt accgacattg gtttcttttt ggtcaccagt tcccttaatt
     6841 tgtgtattac cacgaacatc caagcggact gaagcataga atttcaaagc acgtccgccc
     6901 ggtgttgttt ctggatttcc aaacatcact ccaacttttt cacgcaattg gttgataaaa
     6961 atggcaattg ttttggtttt attgatagag gcgccaagtt tacgcatggc ctggctcatc
     7021 atacgagcct gcaaaccaac atggctatct ccgatatctc catcaatttc cgcacgagga
     7081 acaagggcag caactgagtc gactacgaca agatcaactg cacctgagtc aatcaatttt
     7141 cccgcaatct caagaccttg ctctcctgag tctggttgag acaagagcaa ttcgtcaata
     7201 ttgacaccaa gggccgcagc ataagctgga tcaagggcat gttccgcatc gataaaagca
     7261 gcaatcccac cttctttttg cgcttgtgca actgcatgaa gggcaaccgt tgtcttacca
     7321 gatgactctg ggccatagat ttcgatgata cgtcccttag gataaccacc tgagccaagg
     7381 gcaatgtcaa gagctaaaga acctgagctc atcacttgca ccttttgctc cgcacgttca
     7441 cccaaacgca tgattgatcc tttaccaaag tctttctcaa tcaatttaag agcgtcattc
     7501 aaggcctttt cacgttctgc cccaaatttt tttgaaattt cttctaattt ttttggtttt
     7561 ttcgccattc tattctccta cattctaatg atcctcagac ctatctacta ttatatcaaa
     7621 aattagtcac ttaataaagc cttgcgaact aggttaaagg catgcataac cgcaatgtga
     7681 cgtacatctg ctcggcttct gcctccaata ttaaccttga taacctcagt tccttgatct
     7741 tgcgccaagc ctatgaagac tgtcccaact gggtgtcctt ctaggctatc tggtcctgcc
     7801 actccagtca aactaattcc aaaatcagac tgggtcttgc ttcgtgcctg ctcagccatc
     7861 ttctgagctg taaattcaga caccacacca tgttcttcca aattcttggc aggaatatcc
     7921 aacatccttg atttttcctc caagctatag gtcacaaaac cacccttaaa tatacttgaa
     7981 attccagaaa aattcgccac ggtagcttgg aaaagacctg ccgtcaaact ctctgcagcc
     8041 gcgatggttt tcccttgcct tttcagttct tctaccacaa tgctggctaa actagtttct
     8101 tccccataac catagcaaaa gtctcgtaaa gaaattcctt cgaaagtctg gcagtccaag
     8161 atttgatttt ccaagatatc cagcgcttga ttcgcctctt cttgactgct agcctttgtt
     8221 gacagacgta gagtgacttc tcctgtcttg gcataagggg ccaaggtagg atcgacctga
     8281 ttatcaatta aatcagccaa aatcgtaacc agctggctct cgccaatccc aaagaaacga
     8341 agaactcggg aatacagctt gctccctgtc atcaacttgg gtagaagtag gtttaagacc
     8401 atgggtttca attcacttgg cggacctgga aggacgacat aggtcactcc gtctacttct
     8461 aattttcctc caacagccag tcctgtttcg tttggcagtg gaatcgctcc ttctacaatt
     8521 tgagcttgtc tttcgttatt cggtgttcgg gcatagtctg gtcgcagggc aaaaaagata
     8581 tccaacttct cctgagcctg aggatcaaag actaatgctt tccctaaaaa tttagctagg
     8641 gtttgtttgg ttaggtcgtc ctcagttggc cccaaaccgc ctgtcaaaat caccagactg
     8701 ctacgttgac tggcaatctc aagcaaagac aagagacgaa cttcattgtc tcctacagcc
     8761 gtctgaaaat atacatctac cccaatctca gctagttttt ccgacaaaaa ctgggcattg
     8821 gtgttgacaa tctgtcctgt caaaatctct gttccaacag caatgatttc tgctttcatg
     8881 tttcctccta cctatctatt cgtatttttt tgaaaaaatc gcaggaattt tcctacgatt
     8941 gattttttat ttgtatcaaa agttaattat cttcatcacc aacaggtgct ctgccaaata
     9001 aatcttcaaa taaaaccgca ttggtttcaa gctgagtaac ttcttcttgt cccaaagaac
     9061 gtcggagtat attttgcatt tccaacatat gtgctcttga aacaatctgg taagaaacac
     9121 cttgaagtat ctctccttca ccctgcaact gctgagtttc aatggtttta aatgaatctt
     9181 tatagcctag caagttaggg atactttttg cagacaaatc aatattggtc tgcatattgt
     9241 cactcaaagc ttttagaatc tcttgataat gaccaacgct atttaaactg agagcttttt
     9301 ccatgacttt ttgaataact tcacgttgac gtttttgacg accataatcc ccctcaggat
     9361 cttggtaacg cattcgtgca tagactaggg ctttttctcc cccaatatgt tgctccccaa
     9421 caccgataga aatagtatta aattcttctt ggtcactgat agaaattggg aaacctagga
     9481 tattattgac tgtaatacct cctactgcat ccactagttt ttgcaaccct ctcatattga
     9541 ccatcacata gcgatcaata tggatattca tcattttttg aatggtttct atagcaagct
     9601 ctgctccacc atctgcatat gctgagttca gtttcgcttc atgagcctga ccattccctg
     9661 attcaatgcg cgtcagaata tcccgctcta aactcatcat tgttgttttt ttcgttttag
     9721 gattcactgt catcaagatc atgctatcac ttctaccgac ccaagtttca gttcgttcaa
     9781 catttccggt gtccactccc attaacagaa tggttagagg ttcagtcgct tcaataacct
     9841 tggtttcttc gccgattttt ttataggttt tagctaaggt ttctgtccct tgttgataaa
     9901 tagtataagc aaaaacacct actcctacta cagttacaga aagtaaagct agcaccattc
     9961 caataatttt tttaaccata tttctactaa cctatcagtt tacccatcaa gtaaacatcg
    10021 ataaatttcc cttcttctat atatgcccca cgctcttggc taccttcaat gacaaagcca
    10081 tgcttttgat aaagatggac tgctgcttga ttacgagttt ggacagtcag ttggagacga
    10141 cgcagaatgc cacttgcttg tgcccactct atcgcttctt ctagcaacaa acttcccaag
    10201 ccattattcc aatatctttt tccaatcaca atgaagagat ctccaatatg acggactctc
    10261 ttacgctgat cagctgtaat atttacaata ccagcaattt tgccatttaa gaatgcaagt
    10321 aaggttatct gattgtccga actagcttgc ttgttgagga atatttccat ctcctcacta
    10381 gtcaagagaa taccatctcc gtctaggctg gtaaagtctg tctccaaact cacacgattt
    10441 aaaaaggcca ctaattcagc tgcatctttg ggctctgctt ccctaatgag caattcatac
    10501 tccatattga agctcctcta acaatttctc agcacgcaaa ccctttgcct gaaaatttaa
    10561 acggcgtcca tctgcttctt ttagaatttc caattctaaa taagcatctg gcaaggcatc
    10621 tcctaagaga tttccccact caataacagt cacgccgcca ccaaagataa actcatccaa
    10681 gtcgatagaa tcagcatctc cttcaatacg ataaacatct aggtgataaa gtggaagtcg
    10741 accttcatac tctctcacga tagtataggt gggactttta atcatttgag aaatctgtaa
    10801 tccttttgca agtcctttag taaaggtcgt tttacctgca cccagttctc cagttaagat
    10861 taaaacatca ttctttgcta atagatggcc caaacgctcc cctaaggctt gcaactcttc
    10921 ttcatttttt gtgtacatac tcttattata ccaaaaactt ttcttttgtg tctattttcc
    10981 tactaaactt atcatcataa catccataaa aaacaggctt tctctaaaag aaaatgagcg
    11041 taacaatgac caatacaaga gctcggaaaa tatgaccata aaaggaaact tccttcttaa
    11101 ccgaatttgg gacaagatag gctgcaaaaa acaagcccag tccaatataa atcagaagtg
    11161 agacaatggt cattggattt cttaagaaaa gaagtgttgc taaaatagtc accaacactg
    11221 tcttttttct gtctagcata gcaagaaaat cgcgcacgta ttttttcaag ggtaaaaaaa
    11281 tcagcaaatc tagcccaaat aggaaaaaga aggatggcaa taaaaagtca actaattctt
    11341 gctgcagcgt atttttgatg aacaagttat ctgacaaaac aagaacagct cctaacaaat
    11401 taattaagag taacatactg taaaaaagct tcaccgactt cttactggct aggacactat
    11461 ggacttcttg cttacgggta taaagataat ttactccagc acagattcct gaaacgaaaa
    11521 ccatgctccc gatgaaaaaa gctgtacttt gtttaaagga caagatgcat tccttccata
    11581 ggaaacagct actcaaactg atttgaatta aagctaacaa aaataagatt ctcattgatt
    11641 tcatcttctc tctcccttcc taccaatcat tatactagga gaaaagagag aactgtttct
    11701 aatcttctca aatgtctctt taagacgcta aacaaacact agagactaat actcaatgaa
    11761 aatcaaagag caaactaggt agctagccac aggttgctca aaacactgtt ttgagattgc
    11821 agatagagct gacgtgattt gaagagattt tcgaagaata taaatttgaa atcatgaaaa
    11881 tccgtcaaac gggtggttgt tttgtctcgc acctcacgga gcgagacgga ctcagagtca
    11941 cataattata aggctgatag tattaatcta actatcagcc ttacaggtta tttaacgttt
    12001 cagaaaaact ataatgtcaa gattaactaa acagtatcta attccttcaa ataattttct
    12061 atcttcatca gcattaaagg attgttataa atcttgcata gctctcttgc ttctatataa
    12121 taatttttga cttgttctct gtctagaaat ttggctccag catttcctac aagaataagt
    12181 agaggagcca attggtagct tgtctgtctt tgtttacaga gttcgatagt ctcaagagct
    12241 tcttggatgg cttcattata tttttccttt gatactaggt agtgagcgta attgtaacga
    12301 actctgatgt agccaaataa aaactcttga tgctcaaaat tttttgtctg atataactcc
    12361 atcaaatgag agtagtttac ctcatattct tgttcacgac ccactaagga atagaaatta
    12421 gatagagtat tcaacgcctt taaataaatc aaagtatttg aggagacttt taataatata
    12481 ttttccaatg aagaaattgc ttcacactta ctgtcatatt gacagaagtc aataatagct
    12541 ttaatccatt caaggtaagt tcggtcttct agtgttagaa aagtacttcg ttcaatctct
    12601 attctataaa tatattccaa attttcataa tttctatcat ctaataggcg agcagataat
    12661 tgcttgaaat tagagaggtt agatttaatt tcaatttgtt cattaaaaaa ataatctaat
    12721 ggcacttcga gtcgttgtga gagtttgaac aaaaggtctg cggagggaat gaaataccct
    12781 ctctcaattt tactaatctg gctctattca caaattcctt ctgcaagagt ttgttgggag
    12841 agtctcaact cttttctttt caatctgaat ttctctgcga gtgtattcat taaagataat
    12901 aaaccttcct atttgcttaa tttcattata aaatttttag ttcaaaatag caagttaaag
    12961 gaataaataa ttctttgcgg taataaacaa aaaaaagtca aaaattacat aaaaatgctt
    13021 ggctaaataa aatatatttg ttaaaataag gataatgaaa aagaaaaggg tttcacgaat
    13081 gaagaaaaat cgtggaattc aaaaattagc tatattagta ttactaggtg tttttatgtt
    13141 tagtaacaca attccttacc aacagtttat tcagaagaat agacaattgg agattcgagt
    13201 ccaatcgcaa aagaagtcca atggtcttga tgttgggaag gctgattgat tttatattac
    13261 tttaaaattc aactatttag agaaaggtat tctgatgaga gcttgtaaca ataccagata
    13321 gatataagaa tggagatgtt tgataaaatg ctttcatagc taaccaactg ttgaaaatta
    13381 tgagttacaa aatctaaagt atttaagatt ggtttataaa attaggtctc tgcctgtaga
    13441 aaggaacgtg cagttagaaa ggaggaaaaa ataggatact ataattcaga ctgagatttc
    13501 taattaaaat atatagttgc atttcgctca ttttcataat atttcattca gaggagtaat
    13561 aatatgttaa aatttagaaa agtagttgca tcatcccttg ccgttttggc atttagtttg
    13621 gctcttcctg ttttggcagt ttctcaccgt ggtggcgagt ggacctatgg aggccatcat
    13681 gatccaaata attggggagc attttcgaat tattatcatg gttcacagta tcactgggcc
    13741 tatgttggaa gtaatggacg taacaatcaa aaaacagttt atgcaggagc acgtagtgcg
    13801 gcctatgcgt ttgttaatac aaattttggg gagcaagtta catttgatgc gggatggtaa
    13861 agaataattg aagaagattc ttttatcaat agttcagtaa gcttgttagt ttatagaggg
    13921 caggggtatt tctgccctct gttttagaat aaggaatgca ctatgaaaaa attatttata
    13981 ctcatatcca acctgcttgc tagtttgttc tttgtttggg tatttaccat ttggactgat
    14041 acatatgtct cctattatta ccccaacgtt gttgtacatg attcttcccc tgaaacaact
    14101 tttcagcatg ttgctacacg cttggaaaaa ctagcagaag aaacagatag ctttattgcg
    14161 attcaacacc aagatcctaa ctcagaaggt actacagttt tttcttatac gacctttggg
    14221 gacggaaagt tgcctgatgg gcttcaggaa aaaaaactag aagatgctca aagtagtagt
    14281 gttgaaacaa actattttgt attcgatgga cacctagata ttcacttgct aagggaggag
    14341 ctaagtcaac ttggcttgac taacatgaac ctgacaattc catctaaact atctacgtta
    14401 atggctatct ttagtaacgg atttcagtta atcagtctat tgattttcat cttaaccttt
    14461 atagcactga ctttaattag tcaaattagc caattacggt cgtcgggcat tcgcctcatt
    14521 tcaggagaga aacggtggtc cattttcctt agaccagttg gtgaggattt gaaagggata
    14581 gctgttggtt tcagtttagc tggcgtgctc gccattctta tgcaaaaaat tctgtcgctt
    14641 ccaacgcaat ccctaatgac cataggagca ggcttgctca gttataatct catcttgttg
    14701 tcgatctccc tgtttttcgc tcaactcttc gcagttggga taaaaaagat acacctgatg
    14761 cagattataa aagggcaagt gcctgtcaga ggaatcatta gtttgatttt aatcggtcaa
    14821 ctattagcga ttattattgt aacgctggga atagggagta gcttaaagta ttcccaagcc
    14881 tggcaacagc atcggattgg acaagaggct tggagtcagg aaaggcaact gattacccta
    14941 tcaatcagcc gtgaaggaac gagtcctggt tttgatgaac aagctcaaag gaaactcaga
    15001 acttggtatc aattgatgga tctggctgtt tcagaacaaa aggctttctt atctagacac
    15061 cagttaattg accgtacttt gcaaaatggc atggcttcct ccaaaaactt gataacctct
    15121 acagaatggc acgattacaa cccgaatggc aatgtcctta tcgtcacacc gcaatacttg
    15181 gagcgtcaaa acattcctgt agatacaact attgagcaaa agatgaatca ccttgatgta
    15241 ggggagtttg tcttattgct gcctgaacac ctccgttcag aggaagaaca ttataaatct
    15301 gtttttgaag acgacttaac cagtcgcatg tctagtcaag atgaacgaca gcaaatgact
    15361 gctacggtag gttatttaga atcaggtcag gatcgttttg tgtataatac gacccctatt
    15421 tcttaccagc agtttttgaa agatccaatc atcattgtta taacacccca atcaactggt
    15481 ccacagtcca ttttgttttg gatagacgca gtacagaact acgttctctt taatcaattg
    15541 tctgatgccc aggagcttat ccagagacaa ggcattgaaa attgggtctc agaaatgcaa
    15601 acaggttacc acaactacat cacattattg gataatattc agagggaacg ttgggtaatg
    15661 ctagcaggag ctgggcttgg gattgcaact tcaatcttgt tgtttaacac tatgaatagg
    15721 ctctactttg aagaatttag acgtgccatt tttatcaaac gcattgcagg tctcaggttc
    15781 ttagaaatcc atcgcactta tctctttgct caactgggtg tgtttttact gggatttgtt
    15841 gcgagtgtat ttcttcaggt agagatagga gttgctttct tagtcttgtt actctttact
    15901 ggtctatctc ttttacagtt acatgtccaa atgcagaaag aaaacaagat gtccatgctt
    15961 gttttgaagg gaggttaata tgattgaact taaacaggtg agtaaatctt ttggagaacg
    16021 agagttattt tcgaatcttt caatgacatt tgaggctgga aaagtctatg ccttaattgg
    16081 ttcaagtggt agcggaaaaa caaccttgat gaacatgatt gggaaattag aaccttatga
    16141 tgggacgatt ttttaccgag gtaaagactt ggccaattat aaatcgagtg attttttccg
    16201 tcacgaattg ggctacctct tccagaactt tggcttaatt gaaaaccaaa gtattgaaga
    16261 aaaccttaag ctaggtctca ttggtcaaaa gttgagtcgg tcggaacagc ggttgaggca
    16321 gaagcaggct ttagaacagg tcggcctgac ttatcttgac ctagataagc gcatctttga
    16381 gttatcgggc ggagaatcgc aacgggttgc cttggcaaaa gttatcttaa agaatccacc
    16441 ctttattctg gcagatgagc caacagcttc aatagaccca gcaacttctc agttgattat
    16501 ggagattctg ctatctcttc gagatgataa taggctaatc attatcgcaa cacataatcc
    16561 agcaatttgg gagatggctg atgaagtgtt cacgatggat cgtctgaaat aaaaatcctt
    16621 gtttttaatt gcacgatgag ttactgaaat attatcataa atcaataatt ggagttaatt
    16681 tagaattgta ctttattaat attgaggtaa ctttttcttg ataaaggaag aaatagtgga
    16741 gaggaagtta gaatgaaaaa attcgacaat tatattgttg agaagccttg cgattctaat
    16801 tcagataaac tgcaaaaaat cttaataatt gaaaatttgg tagatgatat tttgcaattt
    16861 cctctcagaa tcaataatat tgtaggagag attttcctcc tacaaccgtt tcaaaagaaa
    16921 actatcttta ttccatgtta ttttgaggaa gatattgtga aagtcaaaga tgatgataaa
    16981 gttgagtgga atttgttaga atttcaaaaa tttagagcat ttttggctta gtaatctgtg
    17041 ttgaaggctc aaaacctatg gtaaaaaagt cgctttgaaa acgtattgcc tccaaagatt
    17101 tagttaaata atgatttaac acaaaaagaa attattgaag ttctggaaag atgttgtttc
    17161 agtattgaga aaaggtggga aaaacttgcg attttcacag agaaaggaag aaaaagtata
    17221 gaaatatagt caattgaaac aagaacagga caaaagaacc ttttgtgcca tatttttctc
    17281 ctttcgcttt acaattggct tgaacacctt tattgtatcg cgtttggagt ttttttggta
    17341 taaccttcga cgcgcacccg catagcgggt gtttttttgt ctcgcaccta acggagcgag
    17401 acgaactaat agtcacttaa tcaaaaaacg caccatatca aaaactaaaa agtttgatat
    17461 catgcgtcat gtcttaaact aattgactat actttctatt caaatgagct tttaaccaat
    17521 tgattgagcc aatccactct taaaaccaaa gagcaatttc tcgcttagct gactcttctg
    17581 aatctgaacc atgtacaaca ttttggataa cctcattttc tccagcagct tttgcaaaat
    17641 cacctcgaat agtgcctggt aaagcttctt ctggacgagt tgcacccatc atggtccgcc
    17701 aagtttcgat tactttggga ccagaaatga cacccacaag aactggacct gaagtcatga
    17761 attcacgaat cggtgggtaa aaactctgac caaccaagtc ctgatagtgc tggtcaatca
    17821 actcttctga aacttgtgaa cgaaactcca atttttcgat tgtaaatcca cgttgttcga
    17881 tgcgctttaa cacttcaccc actagccctc tttttacacc atctggtttg atgataaaga
    17941 atgtttgttc catacccgtc tcctttgtca gcttctttct tttattttac cacatttcgt
    18001 ggaaaaatgg agaaagtttt cagaatagag aatgagagaa cccgagggtt ctctcattct
    18061 ctattattct actgtttctt ccacagtttc aacggcagta tccacaacta cttctgttgt
    18121 ttcttcattt ccttcttcct ctactggagg attaaggtat tcttcttcgt tgacagcata
    18181 tggttcaagg ttacggtaac gggccatacc agtaccagct gggatgatct taccgatgat
    18241 aacattttct ttaagtccaa ggagatggtc tttcttacca cggatagctg cgtcagtaag
    18301 gacacgagtt gtttcctgga aggaagccgc tgacaagaaa ctgtttgttt caagtgaggc
    18361 tttggtaatt cccataagga ctgggcgacc tgtcgctgga actccacctg cgataaggac
    18421 atctttgttg gcatctgtaa agtcattgat atccatgagg gtacccatga gaagatctgt
    18481 atcacctgga tccatgacac ggactttacg gatcatttga cgaaccatta cctcgatgtg
    18541 tttgtcaccg atttctaccc cttggctacg gtaaactttt tgtacttcac cgagaaggta
    18601 cgtttcaact gacaagacat cacgaactgc aaggagacgt tttggttgga tagaaccttc
    18661 tgtcagagca gcaccacgcg ctacttggcc cccaacttcg acacgcatac gagctgtaaa
    18721 tggaacgaca tattcacctt cgccagtttc acccttaaca aagactttct tggtacgagt
    18781 tgatgcatct tcttcgatag cagtaacttc tcctttaacc tctgtgataa ccgcttcccc
    18841 tttaggattg cgggcttcaa agatttcttg gacacgagga agaccctgag tgatatcggt
    18901 atttgaggca accccacctg tgtggaaggt acgcattgta agctgtgtac caggttcccc
    18961 gatagattgg gcagcaatgg taccaactgc ttcaccaact tcaaccgcat caccagtcgc
    19021 caagttgata ccgtaacagt gacggcagac accgtgacga gtgttacatg taaatacaga
    19081 acggatagtc acttcttcca caccagcatt gacaatttca cgcgccttgt cttctgtaat
    19141 caattcattt ggaccaataa tcactgcacc agtttctgga tgtttaacag ttttcttagt
    19201 gtaacgaccg ttgagacgct cttcgagaga ctcgatcatc tcttttcctt ctgcgataga
    19261 acggatcaag agaccacggt cagttccaca gtcgtcctca cggatgataa cgtcttgggc
    19321 aacgtcgacc aaacgacgag tcaagtaacc tgagtcggct gtcttaaggg ccgtatcggt
    19381 cataccttta cgagcaccgt gagttgagaa gaacatttcc aataccgaca aaccttcgcg
    19441 gaagtttgaa aggattggca attccatgat acgtccattc ggagcagcca tcagaccacg
    19501 cataccggca agctgtgaga agtttgagat gttaccacgg gctccagagt ccatcatcat
    19561 aacgattggg ttcttaggat cttggttagc aatcaagcgt ttctcaagtt tttcacgggc
    19621 agcacgccat tcagctgtaa cagcattgta acgctcgtcg tctgtgatca taccacgacg
    19681 gaattgtttg gtgatttgtt cgacacgttt gtgtgattct tcaatgattt cagtcttgtc
    19741 atcaacgact gggatatcgg caatacccac tgtcaatcct gcaagagttg agtggtggta
    19801 accgaggttc ttcatgcggt caagtagggc agaagtttct gtcgtacgga aacgtttgaa
    19861 gatttcagcg atgatatttc caaggttttt cttcttgaat ggagggttga gctcaagatt
    19921 gctgatagct tccttgatat ctccaccaag tggcaagaag tatttagctg gaacaccttc
    19981 tgtcaagttg gcattgtttg gttcttgcaa gtatggtagc ccctctggca tgatatcgtt
    20041 gaagagaatt ttaccaactg ttgtaagcaa gaccttatgt ctttgctctt ctgtccaagg
    20101 cttgttgagg ctgtctgttg cgataccaac acgtgagtgg aggtgaacat aaccattgcg
    20161 gtaagccata accgcttcgt cacggtcttt gaagaccatt ccttcacctt cgcgaccagc
    20221 ttcttccatg gtcaagtagt agttacccaa aaccatgtcc tgagatggag taactaccgg
    20281 tttcccatct ttcgggttca agatgtgctc agcagctagc atgaggatac gagcttctgc
    20341 ttgtgcttct tctgaaagtg gtacgtggat ggccatttgg tccccgtcaa agtcggcatt
    20401 gtaggcttca cagacaagtg ggtgcaagcg aagagcctta ccatcaatta agactggctc
    20461 gaaggcttgg atacccaaac ggtgaagggt cggtgcgcgg ttcaaaagca ctgggtgttc
    20521 tttaatcact tcttcaagga tatcccagat acgctcatct ccgcgttcca ccaagcgttt
    20581 agctgctttg acgttttgca cgatatcacg ggcaacgatt tcacgcatga caaatggttt
    20641 aaagagttca atcgccattt cacgtggcac accacattgg tacatcttaa gagttggacc
    20701 aacggcgata acggaacgtc ctgagaagtc aacacgttta ccgagcaagt tttgacggaa
    20761 gcgtccttgt ttacctttaa gcatgtggct caatgatttc aatggacggc tacctggtcc
    20821 tgtgattgga cgaccacgac gaccattgtc aatcaaagcg tcaactgctt cttgaagcat
    20881 acgtttctca ttttgaacga tgatacctgg tgcatttaac tcaagcaaac gagccaaacg
    20941 gttgttacgg ttgataacac ggcggtaaag gtcattcaag tcagatgagg caaaacggcc
    21001 accatccaac tgcaacattg gacgaagatc tggtgggata accggaagga tgttaagaat
    21061 catccattca ggtttgtttc cagacttgta aaaggcatcc aaaacatcca aacgacggat
    21121 ggctttgaca cgtttttgtc cagtagctgt tttcaattct tctttgagtt cagcaatttc
    21181 tttttcaaga tctacttgct tcaaaaggtc ttggatggct tccgcaccca tcttggcaac
    21241 aaatgaacca taaccatatt cgcgcaagcg ctctcggtat tcgcgctctg tcatgataga
    21301 cttgtgctca agtggtgtat ccttaggatc aatcaccaca taagccgcaa agtagataac
    21361 ttcctcgagg gcacgagggc tcatatcaag ggtcaagccc atacggcttg gaatcccctt
    21421 gaagtaccag atgtgagata caggagcttt caattcgata tgtcccatac gctcacgacg
    21481 aactttcgta cgcgttactt caaccccaca gcggtcacaa acaattcctc tgtaacgaat
    21541 gcgtttgtac ttaccacaag cacattccca gtcttttgta ggaccaaaga tcacttcatc
    21601 aaagagtcct tcacgttctg gtttcaaggt acgataattg attgtttcag gttttttgac
    21661 ttctccataa gaccatgaac ggactttact tggagaagct agggtgattt gcatactttt
    21721 aaaacgattt acatcaacca ctatttcttc cctttctatt ctaagtaaac tgcttattcc
    21781 tgttcagcag cttcttctgt tgcttccgct tttgttgctt tctcagcttc ttcagcttca
    21841 aaggctgctt tagcctcttg ggctgctttt tcgcgggctt tttcaaggtc atctacgtgg
    21901 atgacatctt cgtccattcc ttcatccaag tcgcgaagtt ccacttcttg gtcatcttcg
    21961 tctaggacac gcatgtcaag accaagagat tgcaattctt tgacaagaac tcggaaggat
    22021 tctggaacac ctggttttgg aattggtttg ccttttgtaa tagcttcata ggctttcaaa
    22081 cgtccgttga tatcgtcaga cttgtaagtc aagatttctt gaaggacatt tgacgcaccg
    22141 taggcttcaa gagcccaaac ctccatctca ccgaaacgtt gtccaccaaa ctgagcttta
    22201 cctccgagtg gttgttgggt aacagttgag taaggtccga ctgaacgcgc atgcaattta
    22261 tcgtcaacca tgtggtggag tttgatcatg tacatgactc caacagaaac acggttatca
    22321 aatggttcac ctgtacgtcc atcgtaaagg attgtcttgg catcgctatc catacctgct
    22381 tctttaacag ttgaccaaag atcttcagaa cttgctccat caaagactgg tgtcgcaatg
    22441 tgaataccaa gagtacgagc tgccatacca aggtgaagct ccataacctg accgatattc
    22501 atacgtgatg gcaccccaag tgggttcaac atgatgtcga ctggagttcc gtctggaagg
    22561 taaggcatgt cttctacagg aacgatacga gagacaaccc ctttgtttcc gtgacgtccg
    22621 gccattttat ctccgacctt aatcttacgt ttttgagcga tgtaaacacg aaccaacatg
    22681 ttaacacctg attgcaactc atctccattt acacgtgtaa agatcttaac atcacgaacg
    22741 acaccatcgg caccgtgtgg tacacgaaga gaagtatcac gcacttcacg agacttgtct
    22801 ccaaagatag cgtgcaagag acgttcttca gctgaaagat ctttctcacc cttaggtgtt
    22861 actttaccta caagaatatc accttcttta acctcagcac caatacggat aatccccatt
    22921 tcgtcaaggt ctttgagggc atcttcacca acgtttggaa tttcgcgagt gatttcttca
    22981 ggcccaagct ttgtatcgcg cgtttctgat tcgtattctt caaggtgaac agatgtgtag
    23041 acatcgtcct tcaccaagcg ttcgctcatg ataacggcat cctcgaagtt gtagccttcc
    23101 caagtcatgt aggcaacgat tgggttttgt ccaagcgcca tttctccatt ttccatagaa
    23161 ggtccgtcag cgatgaaatc gcctttttca acgacatcac caacttttac aagagtgcgt
    23221 tggttgtaag cagtacctga gtttgaacga cggaattttt ggatgtggta aacatccaat
    23281 gaaccatctt cacgacgaac ttctaccttg tcagcatctg cgtaagtaac tttaccatca
    23341 tactgagcaa tcacagccgc accagaatcg tgggctgctt ggtattccat accagtacca
    23401 acgtaaggtg cctgaggatt aatcaatggc acagcctgac gttgcatatt ggctcccatg
    23461 agggcacggt tggagtcatc gttttccaag aaaggaatac atgctgtcgc aacggcaact
    23521 acctgttttg gtgaaacgtc catgtagtca acaatattag ctggatactc ttggttgacc
    23581 ccttggtgac gtcccatgac aatcttctca gcaaaggttc catcttcttt cagacgagag
    23641 ttagcctgag ctacagtata ttcatcttct tcatcagctg tcaaccaaac aatttcgttc
    23701 gtgacaacac ctgtttcacg gtcaacctta cggtatggtg tttgaacaaa accatatttg
    23761 ttcaagtgtc cgtaagatga caagttattg atcaaaccga tgttaggtcc ttcaggtgtc
    23821 tcgattggac acatacgacc atagtgagtg tagtgcacgt cacgtacttc atatccggca
    23881 cggtcacgag tcaaaccacc aggtcctaag gctgacaaac ggcgtttgtg agacaactca
    23941 gaaagcgggt tgtgttggtc catgaactgt gacaactgtg atgaaccaaa gaattcttta
    24001 actgcagctg ttacaggacg gatattgata atttgttgtg gtgtcaagac ttcattgtcc
    24061 tgaacagaca tacgttcacg gacattacgt tccatacgag aaagtcccaa acgtacttgg
    24121 ttggcaagca attcaccaac cgcacggata cgacgatttc caaggtggtc gatatcatct
    24181 acacggccaa gtccttcagc caagttgagg aagtagctca tctcagcaag gatatctgca
    24241 ggagtcaccg tacgaacctt gtcatctggg ttagcattac caatgatcgt tacgacgcga
    24301 tctggatcag ttggagcaac aaccttgaat ttttgaagaa caacaggctc agtcacaacg
    24361 gctgcatcgt ttgggatgta gacaatcttg ttcaagtcgc catccaaatg gctttcaatg
    24421 ctttcaatca cgctacgagt cataatcgta ccagcttcta ccaagatttc tccagtttca
    24481 gggtctacca atggctctgc aatggtttgg ttgagcaaac gtgttttaac attgagtttt
    24541 ttattgattt tgtaacgacc aactgctgcc aagtcatagc gacgtgggtc aaagaagcga
    24601 gccacaagca agctacgtga gctttcagct gtcttaggct cacctggacg aaggcgttcg
    24661 taaatttctt tcaaggcttc gtctgtacga gagtccattg gattcttgtg gatatctttt
    24721 tcaacagtgt tgcgaaccaa ttcgctgtca ccaaagatat caaagatttc atcatcacct
    24781 gagaaaccaa gagcacgaac caaggttgta aatggaatct tacgagtacg gtcgatacga
    24841 gtgtaggtga tatcttttga gtcgctttca agttccaacc aagctccacg gttagggata
    24901 acagttgaac catagcccac cttaccattt ttgtctactt tgtcgttaaa gtaaacacct
    24961 ggtgagcgga ccaactgaga aacgataata cgttcaccac cattgatgat gaaagtaccc
    25021 atttctgtca tgattgggaa atcaccaaag aaaacttctt gggtcttgat ttcgcctgtt
    25081 tctttattga tcaagcggaa ggttacaaaa attggtgctg agtagctagc atcgtggata
    25141 cgagcttctt ctagcgtgta ttttggttcc ttgatttcat atccaacaaa ttccaactcc
    25201 attgtgtctg tgaagtttga aattggcaat acatcttcaa acacttcctt aagaccgtgg
    25261 tctaggaaag ctttgaatga gtcagtttga atttcaatca aatttggtaa gtcaagaact
    25321 tctttgattc ttgaaaaact acgacgggta cgatgtttcc cgtattgaac gtcatgtcct
    25381 gccaagatga ttctcctttg taaataagtt ccaagccttg tcaatcaggc ttttctaatc
    25441 gccatatggt tgtaaacccc ttatcaccgt gtcctcttga cgaattttca gaatctttaa
    25501 gcctctgtta caaatgctca aaatcttgaa aaaaagcaca aaaagagcag ctaaatctga
    25561 ctttttcaga agatttaact gctgtgagcc ttgtctggac aatatttcag acaaaaccta
    25621 cgacaaatga ttacccatat tataccctat ttagctagat ttttcaaggg gattcagtag
    25681 gtttttggta attttttccc atagaaaact tggcatcaca ttcgaatcac gctatggtac
    25741 aaaaaactga aaaaactatt gactgaaaat cattttcaag gtataataat aaacgttaag
    25801 gcggtatagc caagtggtaa ggcacggctc tgcaaaagct tgatcgtcgg ttcaaatccg
    25861 tctaccgcct tctataactt gatttatcag gtttcaaatg aacagaaagc ccaatttgaa
    25921 gggctttttt tattttccct cgaataaata cgtataactt taaaaacttt tggagcgagt
    25981 ttgtggcaga gttctttcca tggcataatt cccttttgaa atcaattgaa aaaagcttcg
    26041 tcacgcctgg tgacaaaagc ctctagttta ttctgtttct tcttctattt cgacttctgt
    26101 gatttggact tttaccttgg taacacgtcc attcttcact ttatcattgg ttaagataaa
    26161 ctgtttgttt tggctgacca attcatagct gagtttctca gtcgttggaa tggttcccac
    26221 tcctgtcaaa taataaccag cgatagtatc aacgtcatca ctttccagtt caacattaaa
    26281 gtaattattg aaatcattga gggtcatagt tccttgaaca atgtaggtat cctcgccaat
    26341 ctgatgaaca tctattgctg ctttgtccgt ttcatcatca atttcaccaa caatttcctc
    26401 taagaggtct tccaaagtca ccaaaccagc cataccaccg tattcatcca gcaagattgc
    26461 catttgtctt tgggtatttc gcagttcttt tagcaagtca tccacaaaaa tagtttcagg
    26521 tacaaaaagt ggatcttgta aaattctctt ccaaacaata ttgtcaaaac cgtccacaaa
    26581 gcctgcctta aggagactct tggtgtgaat gattccaatt acattgtcct tatccccatc
    26641 ataaaccggg atacgagaat aattttgttt taaaatactt tggataatgg cttgactatc
    26701 atcctgaata tccaccataa aggcatccgt tcgaggaacc ataacctctc gtgccatcag
    26761 ttcatcgagc gaaaagacac cttgtaacat ctcaatctca tcagcatcca atgtttcttc
    26821 actatttgtc agcatatagg caatttcatc acgggtcatc ttttcatccg catcatcgaa
    26881 tgacatagga gtcaaatggc tcaagaaatt ggtcgaagca gctaaaagcc aaacaaaagg
    26941 actgactagt tttccgatcc caatgataac cggcgctgta cgaattgcca aggcatcctt
    27001 tagattaaga gcgattctct taggatataa ttccccaaaa acgatggaaa tataggtcaa
    27061 aaatgccaag gatagaaaag ttgccacggc ttgtgctgtt tcgccattcc caagccaaga
    27121 ggcaatcaca cgtcctagag tatcagttaa actcgcccct gataagattg taatcagggt
    27181 gattcctacc tgaatggttg ataaaaagtg gttaggattt tctagtacct tcagcaggcg
    27241 gatgtagcgt ctgtctcctt cttccgcctt ttgttcaact cgggcacgat taagagaaac
    27301 catggccatt tctgtggctg agaaaaaggc atttaatacc gtcaagataa acaataaaac
    27361 aaattgtagc aacaaattct gactacttgg gtcttccata gttcttctcc taaaatagta
    27421 tcttatcctt tattatatca caaaatttaa tccaatacat attatttttt tcttagcgta
    27481 acttccatct ctctcccaaa actggaagtt agtctaagac ggatttccac tgatttcacg
    27541 cgtttttctc atactaaaag ctaggttgtg ggaaactgga agttagtttt agaagttact
    27601 attttttctt agtacctgtt ctagccctgc ttaaactagt aaaaagaact cctacatcca
    27661 taagtgacat aggagtttag gcttatcttt tactgagtta cagttacttc tccagttcgg
    27721 taatccagtt gaagtccctt ttgaacattg ggaactagaa cattgaattc caattctcca
    27781 tccgaagact tggcttcaat ctgtgaagct gagggaacta aatcctcgtt tgaagcgtag
    27841 taaagggtta cacggtaacg gacacgcttg ttttggtcca aggctttacg caccttgctt
    27901 tcatagtagt tttgaccagt cgaatactcg gcttgtgcct gatttgccca ggctgtctga
    27961 acagcaatgt ttttaggatt gcttgttgag gcatcaaaac catccaaacc accgattaag
    28021 gcatagccta acaaatgacc tctatcgact gcatgggtat aagagccctt tagattcttg
    28081 acctgatgcc aacctggagg agtccaagaa gttgaaccat tcccagtttc tttacgattc
    28141 ttgtactgac gagtggcctt agacaagagg gcattagcta cggttggaac agtttcctta
    28201 cccactgtct ttgttttatt gtcagcgtag ggcttacttg aaaccttggc atctagattt
    28261 gttttattac cattgacgat aaaagcacct gagccattcc actccagact cccctttatt
    28321 tgactcttga ctgcgtctgt taagacactc tctgccaatg cttgactagg agcttcaggc
    28381 gcttgttttt tctgactaag cttggtttta ggactattag ctgtcgactg catctgcttg
    28441 atataatagc tccctgtaga caaaagcaat aacactagca gtccgattag tgtctgtctt
    28501 gtttttttgt tcatatttct ccttatcttt agaaaaaggc tggttctcca gcctaatttc
    28561 ctgtaaattt atgaatcaat tcctgccatt ttgctggaga caggatagcc catgaatctt
    28621 gacccttgcc caagattcca taacctacca ttaaaccgat tcctagagcc agagtaccta
    28681 aaatcagtac tatgatgact aaaagtaaac gcttgactac gtagcttgat ttcttattca
    28741 tcttcatcac ttgcctcaat ccgctgaatc ttagcaccta gctgcgccaa cttctcatgg
    28801 aaaccgtagt aacctctatc caagtgaacc aatttaccga ccacagtttc tccctgtgct
    28861 accaaacctg tcaaaatcaa agctgcactg gcacgaaggt cagttgaaag aacttctgct
    28921 ccctgcaaag gctgtccacc aacaatacga gctgtatcac ggataatctc agaatgcaag
    28981 cccatgcggc gcatctcttc taggtgttgg aaacgatttt caaaaactgt ctccaccatg
    29041 gttgattcgc cttttgcaac tgtcatcaag gctgtaaatt gagcctgcat atctgttgga
    29101 aatcctgggt ggggcaaggt tttcacatga acagctttta gattttccag ttgagaacga
    29161 acacgaattc cttcgtcttc ttcaattact tcaacaccca tttcaagtaa cttggcaatc
    29221 aagggacggt tgtgctccca gacagcgtct cgaatcaaga catcaccacc agtcatggca
    29281 gcagctacca taaaggttcc tgcttcgata cggtcctgga ctacattgtg agtcgtacca
    29341 tgaagtttct caacaccagt aatggttata gtctctgtac cagcaccttt gaccttggct
    29401 cccatttcat taaggagaat ggctaagtca acaatctcag gctcacgcgc agcattctca
    29461 atcactgtca ccccatcagc cagagtcgct gccatcatca agttctgcgt tgcaccaaca
    29521 cttggaaagt ccatatagat atgagcacca tgcaagcgtt ctgcctttgc ttcgatgtaa
    29581 ccagctgtct gactaatctt aacccccata gcttccagac ctttcaaatg aagatcaata
    29641 ggacggctac caatcgtaca accacctggc atggatacct tggcatgacc cacacgggca
    29701 aggattggcc ctaatacaac gatggaggcg cgcatcttac tgacatactt gtaaggggct
    29761 tcctcagtga tgtcgccagt agcatccacc ttgacaagat gagcttcctc atcaaagtca
    29821 accttggcat tcaaaccacc aactacctga ttcataataa agacatccga caaaatcgga
    29881 acattctgca agacggtctt tccttcactt gctagaatag tcgctgccaa caagggtaag
    29941 actgcatttt ttgctccctc gatcgtcacg cttcctacca gacgattatc gccaccttga
    30001 accacaattt tatccatact cgtttccttt actcttgatt ttataataat cctctaagcg
    30061 cttcttgcca caaatgatac aggctaataa agaaagaact caggatgtag cctatgacaa
    30121 tgctaaaaaa ggctactaat acacgaactt ttcttgtatt ctcagctgtc acttttaaaa
    30181 ccttttccca tctaacaaga tcctttaaaa ggtaaaaact cacataaata aagagcatat
    30241 gactacttag agtgaataat aattgaacca tttgactatt ataccatctt ctctgtttgt
    30301 gcgctagttt ggaaacttca ctcaaaaatt agggattgtt tttcaagtaa tcaatcgcat
    30361 cttgtagggt tttaacgggc acgattttca tatccgtctt gattgtttta gccgcttcta
    30421 gggctgtttg gtagttgttt ttcgcgtccg gatgcgcctt ttgttcttct tcgctaacag
    30481 ggttatcagg agcaaagaaa atagcagcac ctgccctagc cgaagctaca accttcttat
    30541 caatacctcc aatgtctccc acattaccat cgcggtcaat ggtacctgta ccggcaacaa
    30601 tacgaccatt acgaagatct gggtgagcta tttgagtata gatagctaga ctaaacatga
    30661 gaccagcact tgggccgcca ataccagctg ttgaaaagct aattgggaca ttgctgatta
    30721 cctctgtacg atcaatcaag ccgattccaa tcccattttt tccattttcc aaggtgataa
    30781 tcttcccttc tgcagacttg gtttgcccat cctcttcata ggtgaccttg acggaatccc
    30841 ctaatttttg agaactgacg taatcaatca agtctttgga actatcaaag gtctgatcat
    30901 tgactgctgt gactgtatca gagatattga gaatcccttt aaaggttgaa ttatccgtca
    30961 cattcaaaac ataaactcca aagtacttga gttcgatatc cttaccagct gtttttagtc
    31021 cttgatactt ggccatattt tgcgatgttt gcatgtagaa ttgattgatt cgcataaatt
    31081 caacatcgga agaaccacct gtagtctcct gagcactacg aatatctgta aaaggtgtca
    31141 accaagcata aatcatatga gctaaagtag catgttgaac accaaccgta acgaattgat
    31201 aggcaccagc ttccttatct tctgtgtcat ttactttaag gacttggcga atatcttccg
    31261 aaccacctgg aacctctata taataaggca agggcactac aaatgccaag aaagtcacaa
    31321 tcaaggccgc aatgacgtat aagggccatc taatcttttt tttcatttct tatttcctcc
    31381 aaaatactct cgggaacata gcaggcaata tcctgaccaa acttcaaaag ctctctaacg
    31441 cctgatgaac tgatatagag atgttcaggt cgactatgta aataaatagt ctctatatca
    31501 gaagacagct gatgattgta gtaatcaaaa ctggcttcat attgcaaatc cgacgcattc
    31561 ctcaaaccac gcactagaca agtagcaccc aatctttttg caacatcgac caccaattca
    31621 tcatgagaag ccacgacttc aacattttcc aaatgtccca aagccttttc tagcccccgt
    31681 ttacgatttt caagagggag aaatccttgt ttgtggggat taaaaaaaat acccacacaa
    31741 agcttatcaa aaagtctgct cgcccgttca atgatatcca gatgcccatt tgtcatcgga
    31801 tcaaatgagc ctgtgaataa gccaatctta tctgacatag actgtcacct tactaattcc
    31861 ataaatcttt tccttccaga tacccagaca ggcaatttct tctggaagtt caacggtctt
    31921 atccgtctcg cacacaacca taacatcttc agaaaaaagc tctctctcag ccattttttc
    31981 aatatctgct acgatttgtt ccttggcata gggagggtct aagaaaacga ggtcaaattc
    32041 cccagatacc tgttccaatg ccctttctgc atccattttg aggagttgaa attttccaac
    32101 ttccttggtc atctggatat tttcagccac gatggtctga gccttacggt ctcgctccac
    32161 caaaacagca ctggacatgc cacgcgatac tgcttcgata gataaaccac cactacctgc
    32221 ataaaggtcc aagactcgtc ccacttcaaa gtagggacca atcatgttaa aaatggctcc
    32281 cctaacctta tccgaagtag gtcttgttgt cttgccttct agtgtcttga ggggacgtcc
    32341 cccatagatt cctgatacga ttttcatact gtttattata ccaaattata gacaaaaaga
    32401 gaaagaaaac cgaaccttgc ggttcgattc tctacaaaat attttcgtaa gtatcgcgga
    32461 cttcttgagg ccaaacactt gtttgcactt ctccgatgtg tctcttgcga agtaggaaca
    32521 tggccatacg agattgtcca attcctccac cgattgtcaa tgggaatagg ccattcaaca
    32581 aagacttgtg ccattccaat tctaagcggt cttcatcacc tgtaatttcc acctgacgtc
    32641 taagagtttc ttcatctaca cgaattccca tagaagacaa ctcaaaggct ccacctaaag
    32701 actcattcca gacaagaata tcaccattta gacccttgta gccattctca gactcgcttg
    32761 tccagtcatc atagtctggt gcacgtccat cgtgcggttt accatctggc aactcgccac
    32821 cgataccaat caaaaagacg gctccaaatt ctttacaaat cgcattttca cgttctttag
    32881 gtgtcaagtc tgggtagcgt tctaccaatt cttctgtatg gataaaggtg atttgttttg
    32941 gcaagataga ctcgatgtca tagcgggctt caacagctag ctcagtcagg cgaatagcct
    33001 tataaatctt ctcaactgtt tcttttagat aaacgatgtt acgcttacca tttgggataa
    33061 ccttctccca gtcccactgg tcaacataaa cagagtgggt tgcatccaag gaatcctcat
    33121 ctggacgaag ggctttcatg tggacaaaga gaccctctcc ttcaccaaag ccaaaacgag
    33181 ccaaggtgtg gcgtttccat ttagcaagtg agtgcaccac ttcataagta gcatcaggga
    33241 tttggagaac cttgaccgat actggatttt ccacaccaga caggttgtcc tgcattccgt
    33301 caccgacctt actcaagata ggaccttgaa cttcgacaac ttctagctta tctttcaaat
    33361 actgggtaaa agtgtttttg acaaaggaaa tttcttcttg ttgatggata aaacttttct
    33421 tcatagacaa ctcctcaaaa atttaataaa cgtattatac acctatttcc aagaaaagaa
    33481 aagagaaaat tttaaaaaaa tcagtgtcac tcaaaacact gatttcatag accttttagt
    33541 cattgcgacc aaagatacgt agaatactta ggaaaagatt gataaaatca agatagatgc
    33601 tgagagccat tgacaccacc cagcctgtcg ctacacgacc ttgtgattgt tcataggcga
    33661 ggcgaatctt ttggttatcc caagcaatca accctgaaaa aaccaaaacc atggctacgc
    33721 taatcatata atcaaagaag ccactagcca agaaaatatt gactaccata gcaatcagta
    33781 aaccaatgag agctgccatc attgctcgac caatcccact caagtctttc ttggtaaaaa
    33841 taccaactgc cgccatgaca aagaagagaa gggcgctcga tacaaaggca gataaaactg
    33901 tacccggagt atagaaagcc accacaaaac taagggtaaa gccgtttaaa acggagtaaa
    33961 gtaaaaatac tggaagagca gctggactat ttcttgaagc catgctactg gcaacgaaaa
    34021 ccaaagccaa ctccgcaaaa gtagcaatgg tcaaccagag acgcccctgc atcaacaagt
    34081 aaaccaactg agactgaaag accgtcaaca taaggcctga taccaaagca gatagtccga
    34141 ttcccagacc aacaaaggca taaaccttag cgtaaaattg attgagacct gcacggtcat
    34201 gtataatagt gtgattcatc cttttctcct tttctgtgaa catctttcct tgattataac
    34261 aaaaaaagtt aaaattagct taaatggaag attaaaatac cggctgcaac tgcaacattc
    34321 aaactctcgg cctgcccctt catgctaata tggaccaact ggtctgcact ttcagccata
    34381 aggggactaa ttccttgacc ctcatttccc ataactagta caaaattttc tatagaaggc
    34441 agttctctgt aatcaacaga atctttagat agggttgttg ctagcactgg gatagctacc
    34501 tttttagttt cgtcaagaag cgcttgacta gtcatccggt aaatgggcag atggaaatga
    34561 ctaccttgca tggaacgtag ggttttgaga ctgtagatgt ctgccgactt atctgaaaca
    34621 atcactcctg taaaacctgc tgcatccgca gttcgaatga tagttcctac attaccagga
    34681 tcttgcacat cttccaaaaa caagaacttg ccctgactca aatcagcttg tcctacttct
    34741 tctttttgaa ccacggcaac aattccctgt ggagtctgag aatccgccaa atctagcaaa
    34801 atatcctctg aaatccagac agtttgcgga aaagcagcta actgatttcg ataattttct
    34861 agggcaaaga ttttctcaat cgtcactcca gcttggacag cttcttcaaa caagtgccag
    34921 ccttcaatca aataggcaga cttgcggtat tttttttggt gtaatttctt ggcatttttt
    34981 accacagaat tggcttttga ggttataata gtcatagaaa tattataaca caatcaaagg
    35041 ggtttggtat gcaaaaggtt agaatgattg cccaaggtag ggtgcaggga gtcggctttc
    35101 gttggggtgt ttatagcctg gcccttgaaa tcggtggcat cacaggtcgg gtatggaata
    35161 acgacgatgg cacagtggaa atcttagccc aagcagactc atctgctatc atggcaaaat
    35221 ttatccaaga aatccgaaaa ggaccgacac ctttttcaaa agtaagctac ttagatgtca
    35281 aactaagcaa ctttcctccc tactctgact ttaaaatcgc aaattaggtc tccagaacta
    35341 ttgtatattt tgtaaaaaaa cagtagaata gaaaggtata atttttaaag aaggaataaa
    35401 aacagtgaaa tctattaaac gttttgcatt ctcggctatg ggagtggcta tgttgcttgt
    35461 cttgactggc tgtgtcaatg tcgataaaac cacaggtcag ccaacaggat ttatttggaa
    35521 tacgatcgga gcgcctatgg ctgaagccat caagtacttc gctactgata aaggtctagg
    35581 ctttggtgtc gctatcatta tcgtaaccat tatcgtacgc ttgattatct taccacttgg
    35641 tatctaccaa tcatggaagg caacgcttca ctctgaaaag atgaacgctc tcaagcatgt
    35701 ccttgagcca caccaaacac gtctcaaaga agcaactact caagaagaaa aactcgaagc
    35761 ccaacaagcc ctctttgccg ctcaaaaaga gcacggcatc agcatgtttg gtggtgtagg
    35821 atgtttccct atcctccttc aaatgccttt cttctctgct atctactttg ctgcccaaca
    35881 tactgaaggg gttgctcaag caagctacct aggcattcct ctaggttctc caagtatgat
    35941 tttggttgcc tgtgctggtg tcctttacta tcttcaatcg ctcctttcac ttcacggagt
    36001 agaagatgaa atgcaaagag aacaaatcaa gaaaatgatt tacatgagcc cactcatgat
    36061 cgtcgtcttc tccctcttct caccagctag tgtcacactt tactgggttg tcggtggttt
    36121 catgatgatt ctccaacagt ttatcgtcaa ctatatcgtt cgtccaaaac ttcgcaaaaa
    36181 agtccgtgaa gaactagcca agaacccacc aaaagcaagt gctttctcta aaccaagtgg
    36241 acgaaaagac gttacccctg aacaaccaac tgctatcaca agcaagaaaa aacacaaaaa
    36301 tcgcaacgct ggaaaacaac gttcgagata agaagaaaaa ggcttagctg atttgctaag
    36361 tctttttgca aatcaaaagc ccgatgtttc catcaggctt cttttctatc catgtacacg
    36421 tttcatataa tcttgataac tttcggtatc catgagtttt ttagcgttct tgacgcgatc
    36481 tgctgttggt ggtttgactc cttcgaggga atatggaatt ccaagttcac gccacttgaa
    36541 ctcacccatg gtgtgataag gtagaatttc aaacttatca acatttttga gggtcttgac
    36601 gaacttacca agttcaatca agtcatcatc tctgtctgtc aatcctggaa ctagcacgtg
    36661 gcgaatccag acaggttttc caatatctga tagatactgg gcacaagcca agatattttt
    36721 attagtttgg ctagtgacaa tcttgtgctg ttcttcgttg atttccttga tatccaaaag
    36781 aaccaagtca gtgacagcca tgagtttgtc aaacttctca aggtaacgtg gtttattacg
    36841 gaaaggaaga gcacaggtat ccaaggtaca gtggattcct tgttccttag ccttggtgaa
    36901 gagagcaatc aggaaatcaa tctgcaagag agcttctcct ccactgactg taatcccacc
    36961 cttatttccc cagaaaccac ggtagcgcaa ggcctctgtc aagacatcat ctaccgtccg
    37021 ttcacgtgac ttattggact ccatagccca agtgtctggg ttgtggcaat actggcaacg
    37081 catgtgacag ccctgcaaaa agacaataaa gcgaatacca ggcccatcta ctgacccaaa
    37141 gctttctgtc gaatgcacca ttcctgtcac ttgtccataa tcaattgttt cttcagacat
    37201 atcgttacct tccttgaaaa cgttttatag ttttattata tcacgacttg aaggaaaaac
    37261 aagatttttg cctatttttt agaaagcaat ctgtttttca atttcatttt caatctatta
    37321 tcagattatt cttcaaaatc gaagccatat aaggttgcaa aatagtcctc agggcgctct
    37381 gcacggcgga tttgacgggc tttaccttct tcggtataga ggatttccgc agaacgtaat
    37441 ttggcattat actggtagcc cattgaaaat ccgtgggcac ctgtatcatg aattaccagc
    37501 aaatcaccga tttctgtatg aggcagttcg cgattgactg caaatttatc attgttttca
    37561 cagagtgaac caaccacatc taccgcttca gctggtccat ctggatgggt cacgttgcta
    37621 atatgatggt aagctccgta catagctgga cgcatgaggt tgactgctga ggcatccaca
    37681 cctagatagg tacggtaggt ttccttctta tgagtcactc ttgtgactag agcaccgtga
    37741 ggtgccagca taaaacgacc caattcggtg aaaatcttga cctgaccaag acctgctgac
    37801 gtaagaactt cttcatacac cttacgaact ccctcaccaa tcaaggcgat atcgttcggc
    37861 tcctggtctg gacgataatt aacaccaata ccgccagaaa gattgataaa gtctagcgaa
    37921 atgcccaact tttccttgat ttcaacagcc agttcaaaga gctgacgagc caactctgga
    37981 taatagagat gggtcacggt attggacgct aggaaggagt gaatcccaaa agtcttggct
    38041 cctttttcct tcaagatagc aaaggcttca aagagctggt ccttggtcat gccaaactta
    38101 gcctccccag gattgtccat aatgtctgtc cccagttcaa aaacgcctcc aggattataa
    38161 cgacaagaga tgatttctgg aatgcctgct actctctcca gatgttcaat atcttcaaag
    38221 gcatccaagt taatggtcgc acccaattca cgtgcatagg cgtattcctt gtctggcgtg
    38281 ttgttggaag agaacataat ctcagaaccc agaaagtcca gtttatggct catcaaaagc
    38341 tctacataac tagagcagtc cacaccacaa ccttcttctt ggagaatttt caaaatagct
    38401 ggagttggag tagccttaac tgcaaaatat tccttaaagc ccttgttcca cgaaaaagct
    38461 tggttgacgg ctcttgcctt ctcacgaatc cccttctcat catacaagtg aaagggagtc
    38521 gggaactcgg caacaatcgc ttctaactct tctctattga taaatggtgt tttcataagc
    38581 ttccttctat tctgtttgca aagaaaggct gggacaatcg ttccaacctt tagttctatc
    38641 tcttatttat cttcgtcaaa gatattgcca acctcaacat cgatggcttg gttcttgaca
    38701 tcaatattgg ttaccttcaa gagtgacttg tagtcaaact gcttttcacg ttcttcttgg
    38761 gcattgtccg caaagaccgc tacacctgcc agttctgagt cgaactcgtg caagagacta
    38821 atcataccat tgaccgttcc gccacctttc aagaagtcat ccacaatcaa gacacggctg
    38881 cctgccttaa gactacgttt tgaaaggaac attttctcga tacggtcacc acttgaacct
    38941 gaaacatagt tgacgctaac agttgaacct tcggtaattt tcaggtcacg gcgcacaatg
    39001 acaaaagaga cattgaggac attggcaact gcatttgcaa gtggcacacc cttagttgct
    39061 acggtcataa ccgcgtcaat tttttggtcc ataaagcttt tggcaataat acgaccaata
    39121 tttttcaaga tggctggtgt gctaagcaaa tcagacagat agatataacc acctggcaag
    39181 atacggtcac tttctgacaa cttggtacgc aagtcttcaa ccatttcctt agcatcctga
    39241 ctcgaaatag acggtgtgaa gatgacacct ccgccagccc cagtcactgt ctggatatga
    39301 ccgatttcaa tttcctcaaa ggcgcgtttg ataatgacga tatcttctga gatggatgat
    39361 ttagcagact catacttttc agcaaaagta ttgagactag ttagtttata aggattatta
    39421 atcaaatagt tggaaatgac aaccatccga tcacttcttc ttaatttcat ttctatttcc
    39481 tattccattt ttattcaaaa aatcaaaaag caaactagaa agctggtcgc tggtggttca
    39541 aaacactgtt ttgagattgt caatagaact gacaaaccct gtaatatacc tgcatatata
    39601 catacgacaa ggcgatacta ccctagtttg aagagatttt cgaagagtat tcatttttgt
    39661 cttttactta ttataccata ttcacataaa aaaacgaaca ttcttatcct aaaaaatgct
    39721 catttttctt aaattatcaa tctaaatctg gtttatagaa ggaacgatta tccatagcga
    39781 agattttatt ggtcatctct cctttatcca ccaaagccag agctgttgac atcatcatca
    39841 tgcttgcatc cagattgtca atcatatgga taatctctgc ctccataata cgtggacgga
    39901 ctggacttcc atattcaagc aagccgtggt gactgaggat gacatgacga agcaaaacga
    39961 cttcttcctt ggtatcatcg atgccgagtt ccataactgt cttggtaatt tcgctatcaa
    40021 tgagagcgat atgtccaaga agattacctc gcactgtgta ctctgtctgg tctggccccg
    40081 tcaactcgat aaccttagct aagtcatgca gcataatccc cgcatagagc aggctcttat
    40141 tgagctgagg ataaacttcg ctaatagcgt ctgccaaacg taccatggtc gccgtatgat
    40201 aagccaaccc cgtttcaaag gcatggtggt tggtcttggc agctggatag gagtagaatt
    40261 ccttatcata cttggtgtag agatttcgga caatccgttg ccagacagga ttttcaattt
    40321 tgaaaatcat ttgcgacatg tagtcacgaa tttccttgac atcaactggt gacttgacct
    40381 tgaaatcggc tgggtcattg ggttcaccag cttgaggcag gcggagagta atttgattga
    40441 cttgaggggt attgttataa acttctcggc gtcctttcat gtggacaacc ttacctgcgg
    40501 taaaggcctc aatgttatga ggttgggcat cccagagctt cccatcaatc tcgccactat
    40561 catcttggaa ggtaaaggct aggtagtttt tcccagctcg agtttgcctc aggtcagctg
    40621 atttgattag gtaaaagcct tcaaatagct catctttttt catgtgacta atcttcatat
    40681 tcttcctcat tttcttgaaa atggagtaga tcaagcgcag gctcaccttc tgacaactca
    40741 atgtgacgga gcgtccgctc gatagctatg gtacgacggt ttaataattc atcaatattg
    40801 ccagaggcat gttggagatg tttttgtgcc ttgaccagaa taccaccaaa cttgccaaac
    40861 tcggtcttga cactggcaag agtcttgctg atatggtcgg cactcttttg gatattaagg
    40921 gtcttgaaac caactgatag agagttaaga agggctgata gggtacttgg tcctgcaaca
    40981 ataatctgtt cttcccgtct caaatcatca aagaagaccg gattgcgcac aatctctgaa
    41041 taaagacctt ctgtcggaac aaacaagatt ccaaagttgg ttgtccgagg tggtgctatg
    41101 tacttgttcc taatatccct agcaaagcgc ttgacgcttg ctaggagtga cttacgacag
    41161 cgttcaatct catccttgtc acctgtctca taggcttctt ccaagcggta ataatctgcc
    41221 agtggaaact tagagtcaat tggcagatag acgtattctt ggtcgccttg tccgggtaac
    41281 ttgatggcat actccactcg ttcactagag ttttcaaccg ttgcgtattc tcgttcgtac
    41341 tgggcaggtg tcatgatgtc ttcaataatt tgccccagtt gcaattctcc cagaatccct
    41401 cgcgtcttgg ttccagagag aaccttgtta agagctccga catcacgggc aactgtctgc
    41461 atttctccaa ggccacgatt gacagactcc agttgtttag aaactgtctc aaaggaagcc
    41521 tgtaagcgtg tctgcaaggt cttttctagt ttttcctcga ccgtctggcg catttgttcc
    41581 aaacgttgct catttgattc ctgcaaggct tggagacgtt ggtctgtctt gtctctagtt
    41641 tggaggagat tatctgtcat ttcttgacgg acttgggtca gaccttggtg caattcaatc
    41701 cgcacttctt gcaaacggtc gctgacaacc acttccaaat ctttttggtc taactggctg
    41761 gcttgtctgg cttggtcaaa gcggtaatcc aactggtctg acaaatgatc tgcctgatcc
    41821 tccaagctct tacttaagtg tttctcctgc ctatcctgcc tttgccaaat cagaaagaga
    41881 ccagctagat tggcaattaa taatagaata agtaaacttt ccatcctacc tcctgtcctt
    41941 gctatgcagt acgaccacat aaccatctgg gcaagttacc gacacttccc tatctatata
    42001 ttcgttagaa gcgtacactt ttttaaagaa aaaattttcc tctgtcaact catacttggc
    42061 tccaagaata gtcagctggc tatcccgaac tggcataaag gctagatagt cgtagtctga
    42121 acgaggttct agctgactga ttccttctgg acaataagta atcaagtttt gcccatcctc
    42181 aatttctatt tgatgcatat aggatgccaa cttaggattg ctaggcagaa agacattggc
    42241 caacatatgg tcaatacggc cacccaaggc accgaaaata gtaacctgag cctgaggatt
    42301 ttgcttaaag atggtcaaga gagccaattc caaatcagta tcatcttttt ctggccgggc
    42361 ttggacaaaa tactgggcac gtttttgaat cacctgtcgc tcttcttccg tcacagaatc
    42421 aaaatctccg actgctagag caagaggcaa atcttcttcc aagatccaga gcgagcctcg
    42481 atccacacca acaaaaccat caaagtccgt ccgataatga ccgcggtctc cgcctgcaaa
    42541 aacggcaacc ctagtccagt tgttttctga gagtttgtac tcgctcattg acatctccct
    42601 taaagacata ggaacctgct acaaaaacgg tcgcaccagc ttctttggct tgagcaatag
    42661 tttggtcatc aatcccacca tccacttcaa tctcaaagtt caaacctttt tcatcacgaa
    42721 gggcaaccag ctcacgaacc ttatccatgg tttctggcag aaaggcttga ccaccaaatc
    42781 cagggttaac tgtcatgact aggacttggt caactagatg aagcacatgc ttaatcgctt
    42841 caactggtgt accaggattg ataacaactg atggcttgac accgagtgaa cgaatctttt
    42901 ggagggcacc atgaatatga ggcgttgctt ccacatggat actaataata tccgcacctg
    42961 cacgcacaaa gtcctctaaa tgatgctcag ggtttgacac catcaagtgg cagtcgaaaa
    43021 ccatcttact atgaggacga agagcttcga ccacacccgc accaaaactg atttgcggca
    43081 caaaatgacc atccataata tcgatatggg catactctgc cccagttgct tctaggcgtt
    43141 taatttcacg ttcaaagttg gcataatctg ctgccagaat tgacggagca atcttgtatt
    43201 gagacatagg tttctcctta ttttggaatt tttttgctga cttttttata ggtttctctg
    43261 cgattttcaa tttcgctgag gaattgcagg tagttgtcaa aacggaaggg cgcaatggta
    43321 ccttcttcaa ctgctggctt gacagcacag gacggctcat gggtatgggt acaagtacgg
    43381 aatttgcagt ctcgactgac agtggcaatc tctggaaagg cctgattgag gtcttcagcc
    43441 gttgatactt cataatccag tgatgaaaag cctggtgtgt ctgcgatttt acccccattg
    43501 aggttgtaaa aactaacagc tcgagtggta tgacgcccgc gacccagact gtctgaaatt
    43561 tctcccgttt caagattaag atctggtgcg attttattga gaagagttga tttcccaaca
    43621 cccgtctgcc ccataaagac cgtaaccttg cctgttaaca aaggcaggag ttcctcttta
    43681 ctggtcacaa agtcatatcc gatgtcacca taggtctgct ggtaaaaatc cagttctccc
    43741 ccgtcttcca acaaatccat tttagaaata tagacgatgg gatgaatacc cttatgctcc
    43801 aaaagaacca agaaacgatc cagcaaattg ctgttaaaat caggttcctt gacagacatg
    43861 attactacag cttggtcgat attgacaata ggcggacgga ccagactgtt tttccgttcg
    43921 tgaatcttga gaatatagcc ttctgaattt tcctcggcag agaaatctac ccagtcccca
    43981 acgtaagggg tatgcccttt tttacggaaa ttcccacgcg cgcgtgtttg atagacttgg
    44041 ccatcactct ctacatagta gaacccagcc aaggctttaa tgatttgtcc ctgcaaaata
    44101 actcctttaa aattgttagt tccattataa caaaaaaaag ctattttagc ttagcttctc
    44161 tttacaattt cgataagtct attctaaaag aaaaagatac actcacgctt tagataccaa
    44221 gttttataca aaaataacga atctacctcc ttatataaaa acaggcgaac agagagccta
    44281 aaactccctg ttcgctttac tttttctgtg tgtaatagaa aaaacctaat aaagcaagac
    44341 aaaaaatatg aatataaact ccatacatca tgtggggaaa gacagactct ataaaatagc
    44401 ctggtagata gatgattgga aattgataga aatcataggt cgttagagat agtaaacaaa
    44461 gaacatacat aaatactgct acaattagaa cccatccttg ctttcgccat agaaaaatca
    44521 tcaataagaa cagacaaagt agatagattg accaatttcg caacataaat tgaaaaccat
    44581 aaatcatact ttcagtcgtt tctccatcaa tcacatcaac tactttatag aaaggtaaac
    44641 aatagctcac taaaaaaaga aggggactaa ttattttttt catattctct accatggatt
    44701 attttgcaag taagctgacc atctatctcc atatcgaatc aaattaacag tataattagt
    44761 tactacccga ttttcacctg gtccaacctt agaaaaacct gtgctatact cccaactact
    44821 tcttgcacct ggatgaaacc atttttgctg ggatgaatac caaccacctt gtctaacttc
    44881 cacacttgaa gattctattt cgatatccct ttcatggaag tcaactcctc cacttatttt
    44941 agtaatattt ttttgtacac ctccatcata actcacccag tgaacaacag tatacaattc
    45001 cacactataa gtactatcaa actgactttg atgagtagta atgttttgag tataagcgtg
    45061 cactttctgt ataccaaaaa tatggtctaa tatattcgtt ggtttagcaa gcttattgta
    45121 accctcttca acaaaagtag ccttctctaa atcagcggta taaacagttt ttccttgtac
    45181 tacttgattg tttatagaag taatacggct atccacaaca atgggggcca ccatatctcc
    45241 atcgctagtt tctattttag ctgcctcttt cacctcatat acaactcgat tttcttcaga
    45301 ctgaatcact tctaccccat ttttttcagg tatagataat ctagtttgcc cattagaaac
    45361 cgttatattt ggaccaatat tcttctcatt tgcataagtc gcaataggaa atcctgatag
    45421 aaccactgca cacataaggc tattgactaa tttttttctc atgaatatat ccttcccatt
    45481 ttcaagagga gtaaaaatag ctaatataag atgaacgttt ccttatattt cagaatagga
    45541 tatctatctg aaagcaattc acactcctat agataactgt tccaaatctt ggctacatat
    45601 atttcaatag tttgctacaa aaaactcctt tctaagtcta ttgaacatgc ccttattcac
    45661 ctactaatat atcacaaaaa taaataaata caagactgtt ttttctacta gttttatgat
    45721 agaatagtat tgttagggta aatttcatat ataaatgtcg agtgaaaaat cgatggagca
    45781 gaaatcggta gacaaagact cgatttcgtt ggtccagccc cacaaggcta atcaggtatt
    45841 tcacgagttt aaaaatctaa tgaagtgccc aatcaactac tgattcattg atttaatacg
    45901 aactatagtg gattgaacct agaaatagta cactaaggat tctaaaacgt ttctagaaat
    45961 taatttgact ttcctaatct ctttgttcat atcttatttc aatctactat atataccaaa
    46021 atgctaggac aaaagtcttt aaaatcagag aaacgcatat tatcaggtgt tggaaaacct
    46081 tgataatatg cgttttatca tagaaagatc tacttcattt tttactaaaa ttaagttttt
    46141 gtccctgcat cttagagtcc ttgcccttta agtgcatctg ctaggctggc aaactcttcc
    46201 aagctgagag cttccccacg cacacttggt gacaagcctg cttggtccaa agccttggtc
    46261 agcttgtcct tgacctcttc agtcttgcca aagtaacctg tcaagttatt ccacaaggtc
    46321 ttgcggcgat gggtaaaact agccttggaa accttaaaga aaaagttctc gtcttccact
    46381 gctacagctg gcactggacg gcgcaccatt ttcaagatgg ctgagtctac atttggtgct
    46441 ggcacaaaga ctgtacgcgg cacgataaag gcaaccttgg ctgtcatgta atactgcaca
    46501 gcgatagaca agctaccgta agccttggta tttggttgag ccgagatacg gtccgccact
    46561 tctttttgca tcatgaccac aaactcacta aaaggaatgc cactctcgat taagtgcatg
    46621 agaataggcg ttgtgatgta gtaaggcaaa ttagccacta ccttgattgg caggtcagga
    46681 tttttgaaat tctggatatg ttgcgccaaa tcgaccttga gaatgtcctc gttgactaca
    46741 gtcacattgt caaaatcacg caaggtatct gccaaaatcg gtaccaaacg atggtcgatc
    46801 tcaaaggcca taacttctgc tgcacgctca gccaaaaact cggtcaaggc accaatacct
    46861 ggcccgattt caatgacatt gacctggtca tcaatctcag ccgtatccac gattttttgt
    46921 aggatattgg tatccgtcaa gaaattttgc ccaaaggact ttttaaaggt aaaaccgtga
    46981 cgctccagca ctgccttggt cacgctataa tctgcaattc tcatctattc tcttttctat
    47041 tatctgttac tactattgta acacattccc cttgtatttt gggggtgaaa atcataccaa
    47101 aaaactatct ttcctcatga agaaagatag tcttggaaat ctttaaaagt ggccttagcg
    47161 cttctctcga aacatacgtt
//
LOCUS       DAVBCN010000007        46070 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVBCN010000007 DAVBCN010000000
VERSION     DAVBCN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46070
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.007"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..128
                     /locus_tag="VQ497_000367"
     CDS             <1..128
                     /locus_tag="VQ497_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2516729.1"
                     /translation="FQSLNQLEQAIVDYIDYYNNKRIKVKLKGLSPVQYRTKSFG"
     gene            complement(257..460)
                     /locus_tag="VQ497_000368"
     CDS             complement(257..460)
                     /locus_tag="VQ497_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2516730.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(453..1535)
                     /locus_tag="VQ497_000369"
     CDS             complement(453..1535)
                     /locus_tag="VQ497_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET2516731.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1661..2443)
                     /locus_tag="VQ497_000370"
     CDS             complement(1661..2443)
                     /locus_tag="VQ497_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HET2516732.1"
                     /translation="MVYTSLSSKDSNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2443..3786)
                     /locus_tag="VQ497_000371"
     CDS             complement(2443..3786)
                     /locus_tag="VQ497_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET2516733.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQSL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(3885..5201)
                     /locus_tag="VQ497_000372"
     CDS             complement(3885..5201)
                     /locus_tag="VQ497_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HET2516734.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5394..6615
                     /locus_tag="VQ497_000373"
                     /pseudo
     CDS             5394..6615
                     /locus_tag="VQ497_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
     gene            6814..8388
                     /locus_tag="VQ497_000374"
     CDS             6814..8388
                     /locus_tag="VQ497_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2516735.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            8652..9440
                     /gene="codY"
                     /locus_tag="VQ497_000375"
     CDS             8652..9440
                     /gene="codY"
                     /locus_tag="VQ497_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HET2516736.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            9440..10015
                     /locus_tag="VQ497_000376"
     CDS             9440..10015
                     /locus_tag="VQ497_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET2516737.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDFFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            10097..10182
                     /locus_tag="VQ497_000377"
     tRNA            10097..10182
                     /locus_tag="VQ497_000377"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10131..10133,aa:Leu,seq:aag)
     gene            10465..11595
                     /gene="ugpC"
                     /locus_tag="VQ497_000378"
     CDS             10465..11595
                     /gene="ugpC"
                     /locus_tag="VQ497_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HET2516738.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            12096..12854
                     /locus_tag="VQ497_000379"
     CDS             12096..12854
                     /locus_tag="VQ497_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET2516739.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGLSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKVLTGEMTLMKLSGMIYDEGWLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            12941..13453
                     /locus_tag="VQ497_000380"
     CDS             12941..13453
                     /locus_tag="VQ497_000380"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET2516740.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            13635..14579
                     /locus_tag="VQ497_000381"
     CDS             13635..14579
                     /locus_tag="VQ497_000381"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HET2516741.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            14588..15265
                     /locus_tag="VQ497_000382"
     CDS             14588..15265
                     /locus_tag="VQ497_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HET2516742.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVRSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            15363..16121
                     /locus_tag="VQ497_000383"
     CDS             15363..16121
                     /locus_tag="VQ497_000383"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET2516743.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            16159..17664
                     /gene="lytC"
                     /locus_tag="VQ497_000384"
     CDS             16159..17664
                     /gene="lytC"
                     /locus_tag="VQ497_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HET2516744.1"
                     /translation="MSVTFFIGEERLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSTEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAESDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTGTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPHYSGKKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            18160..18678
                     /locus_tag="VQ497_000385"
     CDS             18160..18678
                     /locus_tag="VQ497_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HET2516745.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            18808..19314
                     /locus_tag="VQ497_000386"
     CDS             18808..19314
                     /locus_tag="VQ497_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET2516746.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            19314..19484
                     /locus_tag="VQ497_000387"
     CDS             19314..19484
                     /locus_tag="VQ497_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516747.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            19516..20748
                     /gene="clpX"
                     /locus_tag="VQ497_000388"
     CDS             19516..20748
                     /gene="clpX"
                     /locus_tag="VQ497_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET2516748.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            20757..21344
                     /locus_tag="VQ497_000389"
     CDS             20757..21344
                     /locus_tag="VQ497_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HET2516749.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            21355..21735
                     /locus_tag="VQ497_000390"
     CDS             21355..21735
                     /locus_tag="VQ497_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET2516750.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            21787..22677
                     /gene="rapZ"
                     /locus_tag="VQ497_000391"
     CDS             21787..22677
                     /gene="rapZ"
                     /locus_tag="VQ497_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET2516751.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            22674..23651
                     /locus_tag="VQ497_000392"
     CDS             22674..23651
                     /locus_tag="VQ497_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HET2516752.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            23648..24559
                     /gene="whiA"
                     /locus_tag="VQ497_000393"
     CDS             23648..24559
                     /gene="whiA"
                     /locus_tag="VQ497_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HET2516753.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(24593..25561)
                     /locus_tag="VQ497_000394"
     CDS             complement(24593..25561)
                     /locus_tag="VQ497_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET2516754.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWTLAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            25697..26554
                     /locus_tag="VQ497_000395"
     CDS             25697..26554
                     /locus_tag="VQ497_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HET2516755.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            26541..27320
                     /locus_tag="VQ497_000396"
     CDS             26541..27320
                     /locus_tag="VQ497_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HET2516756.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            27344..28696
                     /locus_tag="VQ497_000397"
     CDS             27344..28696
                     /locus_tag="VQ497_000397"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET2516757.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAIQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            28770..29144
                     /locus_tag="VQ497_000398"
     CDS             28770..29144
                     /locus_tag="VQ497_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HET2516758.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            29146..29994
                     /locus_tag="VQ497_000399"
     CDS             29146..29994
                     /locus_tag="VQ497_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2516759.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            30271..31038
                     /locus_tag="VQ497_000400"
     CDS             30271..31038
                     /locus_tag="VQ497_000400"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET2516760.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDDMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            31050..32234
                     /locus_tag="VQ497_000401"
     CDS             31050..32234
                     /locus_tag="VQ497_000401"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HET2516761.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            32231..34102
                     /locus_tag="VQ497_000402"
     CDS             32231..34102
                     /locus_tag="VQ497_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2516762.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDSKIRPFFGHYTDYLDEKAFETDMANQVQKVEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(34245..35429)
                     /gene="mntE"
                     /locus_tag="VQ497_000403"
     CDS             complement(34245..35429)
                     /gene="mntE"
                     /locus_tag="VQ497_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HET2516763.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPISEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            35733..38429
                     /locus_tag="VQ497_000404"
     CDS             35733..38429
                     /locus_tag="VQ497_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET2516764.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            38442..39233
                     /gene="yghU"
                     /locus_tag="VQ497_000405"
     CDS             38442..39233
                     /gene="yghU"
                     /locus_tag="VQ497_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HET2516765.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            39349..39759
                     /locus_tag="VQ497_000406"
     CDS             39349..39759
                     /locus_tag="VQ497_000406"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET2516766.1"
                     /translation="MEKKIVKDILFLSQVSHPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGMQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            39771..41366
                     /locus_tag="VQ497_000407"
     CDS             39771..41366
                     /locus_tag="VQ497_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516767.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
     gene            41533..41697
                     /locus_tag="VQ497_000408"
     CDS             41533..41697
                     /locus_tag="VQ497_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HET2516768.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            41776..42504
                     /gene="yaaA"
                     /locus_tag="VQ497_000409"
     CDS             41776..42504
                     /gene="yaaA"
                     /locus_tag="VQ497_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HET2516769.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(42555..44039)
                     /locus_tag="VQ497_000410"
     CDS             complement(42555..44039)
                     /locus_tag="VQ497_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2516770.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILERLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEGYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYISGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
     gene            44255..>46070
                     /locus_tag="VQ497_000411"
     CDS             44255..>46070
                     /locus_tag="VQ497_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2516771.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAVSVLLGTALVLGAAASV
                     QTVQAEENKQETTNSISVGRGEAATKPAEVSASNKEKTYAAPTVANPVETTPVKTGEV
                     TKPAEKVEEAKDKKEEVTHQDAIDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADASEAELSAAESSIQSSIIGLELRSNSNKGTVSETPVAKKANIVEAKEETN
                     PAATTDRSAVDSAVLPTSTAAKVETTSAPASINEILKPGLSLSDARQNPAIRKEDVDR
                     GYSGFRAAPAPTNQPVTNLGNNTVFTDISRGSHTFRGNGNSRRGNPIHFDVTTTRVRN
                     RVDFSIRYSGPGEFVNNNFILDKGNGFGEPSRATVSTPKLRDQVKNISKGGNFVSHSG
                     YSLEAAVETSTNQTIKFSLPIANPNGDLSVRLRPVTFNVGQGESGSATSNDPYSNSNY
                     YFREKPLLLDANPNGGTNNKTVSEDIDFQTVYLPTSKLPEGQTRLVREGEKGQRQIIY
                     KVHQLGNETILGLPISNSVIKEVKPRIMQIGVAKDLIDTVKPRVDQNKVGDTNNLTFY
                     LDNDGNGVYTEGVDELVQKIAIKDGAKGEKGDRGERGLT"
BASE COUNT        13718 a         8529 c        10409 g        13414 t
ORIGIN      
        1 cgtttcagtc gcttaatcaa ttggaacaag ctattgtaga ctatattgat tactacaaca
       61 ataaacgaat taaggtaaaa ctaaaaggac ttagtcctgt gcaatacaga actaaatcct
      121 tcggataaat taattgtcta actgtttggg ggcagtacat attcaacacc tgatactatg
      181 cgttttattg tgggaagatt ttcctcattt tctagtgaaa ttgaactttt tctaacatct
      241 tttgatagat gacttcttaa tcaattatca tttccattgt tgtttcttgg ggtttcatcc
      301 cttgtctttg ataaaagcgg agagcaccct cgttatcatt ccagacactt agggttagat
      361 tattgcagcc aatttcttta gcataatagc agaaaaatga caataaaata gcccctagat
      421 attttgaaag gaaagcatag gggctatata gtttaatcaa aataaagcaa atctttgctg
      481 gtgcttgtaa agaggtcgaa gccatccttg gtaacaacac cgcagtcttc aatacgaaca
      541 ccgactttac cagggatata gatacctggt tcaacagaga agcacatgcc ttcttcgatg
      601 accatgtcgt ttccttccat gatagatggg aattcatgga catccatacc gataccatgc
      661 ccgagacggt ggttgaagta ctcaccataa ccagcttttt cgatgacctc acgggcagcg
      721 cggtccactt catgagcagt cacacctggc ttgataaagt caagagcagc ttgttgggct
      781 tcaagagtca agttgtaaat atctttcttg aattggtctg gtttgccgac agcgactgta
      841 cgagtcatat ctgacgcata gccattgacc agaacaccca ggtcaaagag gagaagagca
      901 tcattttcaa ccttattagc tgctggaatg ccgtgtggat tcgcagcatt atcaccagtc
      961 aagaccatgg tatcaaagct catttcataa ccttcacgtt tcatggcaaa gtcgatttgt
     1021 gcgatgatat ctgtctcagt cttatcaaga gaaatattgt caaaaccaac atgaacagcc
     1081 ttgtcagcat aaagacctgc aaccatcatt ttttgcactt catcagctga tttgatgagg
     1141 cgcatgcgtt ggatacgagg agtgaggttg tcaaactcag cagtctcaaa aactgttttc
     1201 aaaccatggt atttggtcaa gatgagattg tcaaactcaa cagcgacacg tttgaagtca
     1261 agttgtggaa gagcatgttt gattttttgc catggatttt cagaatcgac atagcccact
     1321 actgggaagg aaacggtgct acttgcacgt tctacttcaa gagctgggac aaagaggaga
     1381 ggttcctgat ccgctaggac aaagaggaac atttggcgtt catggggatc actgtaaaaa
     1441 ccagtgaggt aattgattgt gacggggtca gatacgacag cgacgtctag tttttctgat
     1501 tcaagatatg ttaggatttg ttgtaattta gacatatgct accttctttc tacccctcta
     1561 ttttggcaaa aaatgagaga aaatgcaagg gagaagggta aaagaaaaga caaaaaactc
     1621 cgacaagata acggagttct ttgatatcat tttcctttgt ttaagaaaag tcagccttgc
     1681 ttttgacgtc actgtactct tcagcgattt cttggctgag aatgtcctca taggcaggga
     1741 gttggatgtc ctgaccatat ttcttcttga gggcagtagt aactaggcga taagccagat
     1801 tggcattacg agagaggata ggcccgtgga agtaggaacc aaagacattc ttataatgaa
     1861 ccccttcacc gaccttttct tcgttgtttc catttccata gacaacctgt cccagcggtt
     1921 tttggtcatc agagaggaag gtacgacctt ggtgattttc aaatccatag taggtttcat
     1981 cgaaatcttc attgtgaatc ttgatgtcac cgataaaacg gttattggtc tggttgagcg
     2041 tgtagtgtcc catgacccct agcccttcga tacgttttcc tgaagcttca acataatatt
     2101 gacccaatag ttggaaacca ccgcagatag ccagaactac accgtcgttt tggatgtagt
     2161 tgtcaatgct ctctttttta gcaggtaggt cgtctgcaat gatactttgt tcaaagtctt
     2221 gaccaccacc gaaaaaggcg atgtcgtagt gattttcatc aaagtcatca tggagagaaa
     2281 cgatgtcaac ggtcacatgg gctcccagtt tttcagccac atacttgagc atgaggatgt
     2341 ttccattgtc cccgtaggta ttcatgagat ttccgtagag gtgggcaatg ttgagctgat
     2401 aggggtaatt gctatctttt gaggaaagtg aagtataaac cattagttca tctcctttct
     2461 aacaatctga cgactagcca gcagttcacg aaattccagc atggcagtat aagttgccag
     2521 aatataggca tgcttgcagt cttgattctc aatggtcttg agaacttgct ccagattact
     2581 cgtttcagtg attttctcag ctggatagcc agtcactcgg aggcgacgag cgatttcaga
     2641 atgacgaaca ccgccagcgt tgatttcagg aatgtccatg tcagtgattt gctcaaagtc
     2701 tgcatcccag atccagctag tgtcaattcc atctgcatag ttggcattaa ggaggacaga
     2761 taggctaaat ggataaggtg ctagtttgat catttcgata gcttgggttg caccgactgg
     2821 atttttaatc aagacaaggg tacattcctt gtcaccgata tgaaaggttt cttggcgtcc
     2881 aaagacagca cggctcttgt caaatccctg tttgatgagt tgggaatctg cgccgaggaa
     2941 acgggcgatg gccacagcag ctagggcgtt atagatatta tagagcccgc cgatttggat
     3001 accgtattct tggccgtcta tgacaaagcg agagcgattg ttggtcaact caaccagttt
     3061 tgtcaaacga tagtcgagat caggacgttt acatccacaa ccttcacaga tataggcacc
     3121 caagtttgca taggtattat gctcatattt gaggatgcct tggcagtcag gacagagaat
     3181 cccttcggta ttgtagtgag ccagttgggc tggtcctttt tccaagtcaa aaccaaaata
     3241 ctctataggg tttggaatag ttggcttgta gaaaagtgga ctgtctccgt taaggagaac
     3301 agtagcagtt ggaacttttc gaatggcatc caatatcatg ttataggtag tatagatttc
     3361 accgaaacgg tccatctggt cacggaagat attagtaatg acaaaaagac taggctggat
     3421 atagtcacag atacgagata gactggcttc gtcaatttcg aggacggcaa tatttttccc
     3481 agtttttgaa gatttggctg ttaggaaggt tgttgcaatc cctgtaatca tgttggcacc
     3541 gcttgggttg gttagaactt gaccataaac ctcttttaaa atgccgacag tgagggcagt
     3601 tgtcagggtt tttccatttg ttccagtgac aacgacaatc tcgtagttct tagctaggct
     3661 ttgtaaaata tctttatcaa attgaagggc gactttccct gggagcgtac ttccacgtcc
     3721 aagacggctt aaaacgaagt gggaagaacg cccagcaaga aggcccaaag tagtttttaa
     3781 gttcatgttt ctattatatc acaaaagagg gaaaagacag atttggtgtt tcgtagaagt
     3841 aaaaacagcc caaagagggc tgtcagttaa gatgagattt caacttaaat cgcaaacaag
     3901 tcattcaagt tctcagccaa ggttgggtga gtgaagattt gttttgtgaa gtaagtgtaa
     3961 ggaatcttgt tgtccatagc aacagtaatg atgttgatga tttcttgaga accttctgag
     4021 aagatgcttg caccaagaat ttcttttgtt tcagtattaa caacagcttt gaaagctccg
     4081 cgaaggtctc catttacgtg accacgaggc atggctgcaa cagggatttc tttcactgcg
     4141 tatggaagtt tcaaatcagc tgcttggctt tcagtcaaac caacttgtga aagtgcaggt
     4201 gtgatgaaca tagtatttgg cacattgaga cggtcctcaa gtgtgtagct gccatctcca
     4261 gcaaggtagc tgtaaacaac acggaagtca tcaagtgaaa tgtaagtaaa ttgaaggcca
     4321 ccgttgacat ctccaactgc aaagacacca ggaacgtttg tttgacagtg tttgtctact
     4381 ttaatagcac cacgttcagt tagttcaata tctgtatttt caagttgaag tggttctaca
     4441 tttggtttgc gtccagttgc gtagagaagg gcgtcgaaac ggtaagtttc gtcttcagtt
     4501 acgacaagca cttggtcacc atcgtttttg atttcagtag tatggatatt ttgaagcaat
     4561 tcaatgccat cttcttccat gtattgttta gcaagagctg cgatggaagg ttctgcacga
     4621 ggtaggaatg tatccaaggc atctaggact gtgaccttgc ttccaagttt gttgtaaagg
     4681 ccggcaaatt caagaccgat atttccgcca ccaaggattc caagtttttc aggtaatttg
     4741 tccaagcttt ggatacctgt tgagtcaaag atgtttttgc ttgtagcaag tccaggaatt
     4801 ggcaagacgt ttgaaacagc accagtgttg atgacgattg tttcagcagt cagttctttc
     4861 ttttcatcac cagcttggat ttcgatgact ttatttgaaa ggaagtgagc ttccgcatca
     4921 aagatatcta cgcctgtacc agcaacagtc gcatagtttt taccgttgag gcgaccagtg
     4981 atcgtgtttt tagtagcaat cacttcttca aaagacaagt ccttttcagc agcaactagc
     5041 aaggttttag ttgggataca accaatgttg atacaagttc caccgtacat agccttgctg
     5101 cgttcaacga gggcaacttt tttgccagct gaagccaatt tacctgctag tgttttacca
     5161 gctttaccaa atccaataac aattaaatcg tatgttaaca tttatcttcc tcctattcga
     5221 atttcattct agcacaagaa aaaaactttt gcacaattct gctacggaaa agacagaaaa
     5281 agataaaaac gctttccaga caaaaaacta gtaatttttt gacaagcttg ataaactaaa
     5341 atcctatcta tttttatata agaaagaata aatgttcgaa aataggaggt tttatgaaat
     5401 cgaatcgtta tattattgcc tttgctgggg tcattttaca cttaatgctg ggttcgactt
     5461 atgcttggag tgtttatcgt aaccctatta ttgaaaaaac gggatgggat caggcttctg
     5521 ttgccttcgc ctttagtcta gcaatctttt gtttgggctt atcggctgca tttatggggc
     5581 gtttggtaga aaaatttggt ccgaaagtca tggggagtct atctgctttt ctatacgcag
     5641 gtggaaatat cttaacagga tttgcaatag accgtcagga actgtggttg ttgtatctag
     5701 cttatggcat tttaggtggg cttggtttgg gagcaggcta tattacccct gtgtcaacga
     5761 ttataaaatg gtttcctgat aaacgtggtc tcgcaacagg tttagcgatt atggggtttg
     5821 gttttgcttc tttattgact agtcccatag cgcaacacct catcgcaggg gtagggattg
     5881 tagaaacttt ttatatttta ggagcaagtt actttattat catgctccta gcttcacaat
     5941 tcattaagcg tccaaatgag caagagcttg caattttatc ttcttcaggg aaagaaaaaa
     6001 cagcctcttt gacgcaagga atggctgcaa atcaggctct aaaaagcaat cggttttata
     6061 tgctttggat tattttcttt atcaacatag cttgtggttt aggcttaatt tcagcggcat
     6121 cgccaatggc acaggagatg gctggcttgt ctacaagtca tgcagcagta atggtgggtg
     6181 ttttggggat tttcaatgga tttggtcgct tgctctgggc gagtttgtct gactatatcg
     6241 gtcgccctct aacctttagt atattactgc ttgtcaatct tttctctctc actttggctc
     6301 tttacagatt ccgttttatt tgtagttgct atgtctattt tgatgacttg ctatggagct
     6361 ggtttttctt tgattccagc ttatctcagt gatatttttg gaaccaagga attggccgct
     6421 ctgcatggtt atattttaac agcttgggca atggctggtt tagcgggacc tattttatta
     6481 gcagagactt ataaaatggc tcattcgtac acacaaacct tgttcgtttt tctcatttta
     6541 tacagtatcg ccttggcttt gtcttattat ctaggtcgtt caatcaaaaa agaaagtcaa
     6601 aaaccgctta catgattttc tgtagaaatg gggctatctt ttgctctggt cttgtgttat
     6661 actagatagg ttgcaaagaa aacagtactt ttcttttgtg gaaaagaagc aacacgattt
     6721 tatatctagt tatatgaaaa tacgtcataa aaagaaaagt ataaactaag ccctataggg
     6781 tggcttcgca ccacctttag aaagaagaat aacgtgaaat ttaatgaatt aaacttgtct
     6841 gctgatttgc tagcagaaat tgaaaaagct ggttttgtag aagctagtcc tatccaagaa
     6901 caaactattc ctttggccct tgaaggcaag gatgttatcg gtcaagctca gactggtaca
     6961 ggaaaaactg cagcctttgg cttgcctacc cttgaaaaaa tccgtacaga agaagcgact
     7021 atccaagctt tggtcatcgc tccaactcgt gaactagctg tccaaagtca agaagaactc
     7081 ttccgctttg gtcgtagtaa gggagtcaaa gtccgttcag tatatggcgg atcaagcatt
     7141 gaaaaacaaa ttaaggctct taaatctggt gcccatatcg tggtgggaac tccaggtcgc
     7201 ctcttggact tgattaaacg caaggccttg aaattacaag acattgaaac ccttatcctt
     7261 gacgaagcgg atgaaatgct taacatgggc ttccttgaag acatcgaagc cattatttcc
     7321 cgtgtacctg agaaccgtca aactttgctt ttctcagcaa ctatgccaga tgccatcaaa
     7381 cgtatcggtg ttcagtttat gaaagcccct gaacatgtca agattgcggc taaggaattg
     7441 acaacagaat tggttgacca gtactatatc cgtgttaagg aacaagaaaa atttgacacc
     7501 atgactcgtc tcatggatgt ggcacaacca gaactcgcta ttgtatttgg tcgtaccaaa
     7561 cgccgtgtgg atgaattgac tcgtggtttg aaaattcgtg gcttccgtgc agaaggaatt
     7621 catggcgacc tagaccaaaa caaacgtctt cgtgtccttc gtgactttaa aaatggcaat
     7681 cttgatgttt tggttgcgac agacgttgca gcgcgtggtt tggatatttc aggtgtgacc
     7741 catgtctaca actacgatat tccacaagat cctgagagtt atgttcaccg tatcggtcgt
     7801 acaggtcgtg ctggtaagtc aggtcaatct attacttttg ttgctccaaa cgaaatgggt
     7861 taccttcaaa tcattgaaaa cttgactaag aaacgcatga aaggtctcaa acctgcaagt
     7921 gtagaagaat ccttccaatc aaaaaaacag gtagctctca agaaaatcga acgtgatttt
     7981 gcagatgaaa ccattcgtgc caactttgag aaatttggta aggatgctcg caaattggct
     8041 gctgagttta ctccagaaga attggcaatg tatatcttga gtctgacagt ccaagaccca
     8101 gatagccttc cagaagtgga gattgcacgt gaaaaaccac taccgtttaa accatcaggt
     8161 aatggtttcg gtggtaaagc taagggaggt cgtggaggcc gtcgtgggga cgaccgtcga
     8221 gagcgtgatc gccgtggcaa tggtcgccgt gatgagttca aaaaaggaag tcgtggcaac
     8281 gatcgttttg ataaggaaaa acgttaccgt aaggataata aaaaaccacg caatacttta
     8341 agcgaaaagc aaacaggctt tgttattcgt aacaaaggcg ataaataaga aaaagcggtt
     8401 caaaatgaat cgctttttta tataaggaga ccgaatgtga aaagacaaat aaaattacta
     8461 tattatcttt tataatgtta tgatacacaa aaataggcaa cttgtcaata gaaaaggaac
     8521 aatttaatta aaaatattct ggtttttaat taaattgtaa gtcaatgcaa gtcaataagg
     8581 aattttcaga taattattga aaaagagacg tttttatgtt ataatgatag cgattaaatt
     8641 cgaggtgaaa aatggcacat ttattagaaa aaactagaaa aattacatca attttgaaac
     8701 gctcagagga gcagttgcag gatgagctcc cttacaacgc tattacgcgt cagttagcgg
     8761 atattattca ttgcaatgcc tgcattatca atagtaaggg acgtctgctt ggctatttta
     8821 tgcgttataa aacaaataca gatcgcgtag agcaattctt ccaaactaag attttcccag
     8881 atgactacgt tcaaggggct aatatgattt acgaaacaga agcaaacttg cctgttgagc
     8941 atgatatgag tattttccct gttgagagta gagatgattt tccagatggc ttgacgacta
     9001 ttgcaccgat tcatgtatcg gggattcgcc ttggttcttt gattatttgg cgtaatgata
     9061 aaaaattcga agatgaggac ttggttcttg ttgagattgc cagtaccgtt gttgggattc
     9121 agcttcttaa cttccaacgt gaagaagatg agaaaaatat tcgtcgtcgt actgctgtca
     9181 ccatggcggt caataccctt tcttactccg aactccgtgc tgtttcagca attttagggg
     9241 aattaaatgg aaatgaaggg aagttgactg cgtcagtgat tgcagatcgt atcggaatca
     9301 ctcgctctgt gattgtcaat gctcttcgta aacttgagtc tgcggggatt attgaaagtc
     9361 gctcacttgg aatgaaggga acctatctta aggtcttgat ttcagatatt tttgaagaag
     9421 tgaagaaaag agattactaa tgacaaaggc tttaatttcg attgattata cagaagattt
     9481 tgttgctgat agtgggaagc tgacagcagg tgctccagct caggcgattt cggatgccat
     9541 cagcaaggta actcgattag cttttgaacg aggagattat atcttcttta ctattgatgc
     9601 tcatgaagaa aacgattttt tccatccgga aagtaagtta tttcctcctc ataatctgat
     9661 tgggacgagt ggacggaatt tatatggaga tttggggatc ttttatcaag agcatggttc
     9721 agacagtcgt gtcttttgga tggataaacg ccattactca gctttttcag ggactgacct
     9781 agatattcgt ttgagagagc gtagagtgtc taccgttatc ttaacaggtg tcttgacgga
     9841 tatctgtgtc ctacatacag ctatagatgc ctataatcta ggatatgaca tcgagattgt
     9901 taaaccagct gttgcttcca tctggcctga aaatcatcaa tttgccctag gtcatttcaa
     9961 aaatacactc ggagctaagt tagtagatga aaatctaaat gaactttctg agtaacctgg
    10021 ttggtgaaaa tgaaaaaatt tgaaaaaaag tgttgacaag catcccaaaa gttgatatac
    10081 tagtaaagta atcgatgcgg ggatggcgga attggcagac gcgcaggact aaggatcctg
    10141 tgaccgcttt aggtcgtgag ggttcaagtc cctctctccg cataggaaaa gagttagcta
    10201 aagctagctc ttttgtattt ctctaaaaaa ttagaatcca gtcaaatgta gtggagaatc
    10261 agcgaaaata tgggaaaact gttccttgtt tctgcttttg tcatagaaga ataataaaga
    10321 ttatcttctt caaattctcc gatattctct aaagttttgt gcaagttgca cagaactttt
    10381 ttattttttt ggtcatcttg ccatagaaat ataaagcgtt ttcatctata atataattat
    10441 caaaagacaa aaggagttca cctcatggta gaattgaatc ttaaaaatat ttacaaaaaa
    10501 tatccaaaca gcgaacacta ttcagttgaa gatttcaact tgaacatcaa agataaagaa
    10561 tttatcgttt tcgtaggacc ttcaggatgt ggtaaatcaa ctacactccg tatgattgct
    10621 ggtcttgaag acattacaga aggtactgca tctatcgatg gcgtggttgt caacgacgta
    10681 gctccaaaag accgtgatat cgccatggta ttccaaaact acgctcttta cccacacatg
    10741 actgtttatg acaacatggc tttcggtttg aaattgcgta aatacagcaa agaagacatt
    10801 aacaaacgtg ttcaagaagc agctgaaata cttggattga aagaattctt ggaacgtaaa
    10861 ccagctgacc tttcaggtgg tcaacgtcaa cgtgttgcca tggggcgtgc gattgtccgt
    10921 gatgcgaaag tattcttgat ggacgaacct ttgtcaaact tggatgccaa acttcgtgta
    10981 tcaatgcgtg ctgaaatcgc taaaattcac cgtcgtatcg gagctacaac tatctatgta
    11041 actcacgacc aaacagaagc gatgacactt gcagaccgta tcgttattat gtcagctact
    11101 aagaaccctg ctggtacagg tactatcgga cgtgtagaac aaatcggtac tcctcaagaa
    11161 gtttacaaaa atccagttaa caaattcgtt gcaggattca tcggaagccc agctatgaac
    11221 ttcatcaacg tgaaattggt tggtagcgaa attgtttctg acggtttccg tttgaaagtg
    11281 ccagaaggag cattgaaagt tcttcgtgaa aaaggctacg aaggaaaaga attgatcttt
    11341 ggtatccgtc cagaagacgt gaatgcagaa cctgctttcc ttgaaacatt cccagactgt
    11401 gttgtaaaag cgactatctc tgtatcagaa ctgcttggtt cagaatctca cctttactgc
    11461 caagttggta aagacgagtt tgttgcaaaa gttgatgctc gtgactactt gcaaacaggt
    11521 gcaacagttg agcttggatt tgacttgaac aaagcacact tcttcgatgt agaaactgaa
    11581 aaaacaatct actaaaataa ataaaattca aagcactaca agaaaagata tctctttatc
    11641 aattgtagtg gagagatatc agttaatcta gggagagaaa caaaatgctt ctctcctttt
    11701 tgctagggaa gtcatattat gcatctatat tgtgatgctc tttaatactc ttcgaaaatc
    11761 tcttcaaacc acgtcaacgt cgccttgccg tacgtatgat tactgatttc gtcagtttta
    11821 tctgcaacct caaagatgta ctttgagcag cttacggcta gtttcctagt ttgctctttg
    11881 atttccattg agtattattt gtgggtacca tctacaagtg aagctatatg cgtaaactac
    11941 gtgagcaatt gaattcgaac tagagaggta ataataaatt tatgctatag ttatggtgac
    12001 ttgtatgctt ttgattctag tttatcaaat aatagattag aattgtcaga taatatcatt
    12061 ttgtgttata atgaagaaaa aacagaggtg ttcaaatgtc agaagcaggt cataagtttt
    12121 tagcaaaatt ggggaaaaaa cgcttacgtc caggtggaaa gcgtgctaca gattggttaa
    12181 ttgcagaagg aggattgtca aaagaaaaga gaatactaga ggttgcgtgt aataggggaa
    12241 ctacagcaat tgagttggca cagcgttttg gttgcaagat aactgctgtt gatatggatg
    12301 ctcaagcttt agaagtggct aaaaaatctg ctggaacggc aggtgttgct catttaatca
    12361 gttttgaaag agcaaatgca atgaaacttc cttatcaaga tgctagtttt gatattgtta
    12421 taaatgaagc tatgctgact atgcaagccg atcaagctaa gaaaaaatgt gtaatggaat
    12481 atctaagggt attaaaacct ggaggtcttc ttttgacaca tgatgtgctt cttaaggaag
    12541 ctaaagagtc tatcagacag gaattatcac aagcaattca tgtaaatgta ggtcctttaa
    12601 ctcaagatgg ttgggaacag gtgatgatag aatcaggtta ttgtgatgtg aaagtattga
    12661 ctggtgaaat gacattaatg aaattatcgg gtatgattta tgacgaaggt tggctaggaa
    12721 ctttgaaaat ttgtgtaaat gcttgtaaaa aggagaatag aaagcagttt ttaactatgt
    12781 ataaaatgtt tgctaagaat aaacagaaat tgggctttat tgcgatggct agttataaat
    12841 cgtcaaaacg ttagataatt attgaagtta acttttcctt ttttctttct taaaaaatat
    12901 gctataatag agagtgaaaa actttgaaag aaagaaaaag atgaatttaa aagattacat
    12961 tgcaacaatt gaaaattatc caaaggaagg cattaccttc cgtgatatta gtcctttgat
    13021 ggctgatgga aatgcttata gctacgctgt tcgtgaaatc gttcagtatg ctactgacaa
    13081 gaaagtcgac atgatcgtgg gacctgaagc tcgtggattt atcgtgggtt gtccagttgc
    13141 ctttgagttg ggaattggtt ttgcgcctgt tcgtaagcca ggtaaattgc cacgcgaagt
    13201 tatttctgct gactatgaaa aagagtacgg tgtcgatacc ttgactatgc acgcggatgc
    13261 cattaagcca ggtcaacgtg ttcttattgt agatgacctt ttggcgacag gtggaactgt
    13321 taaggcaact atcgagatga ttgaaaaact tggtggtgtt atggcaggtt gtgccttcct
    13381 tgttgaattg gatgaattga acggccgtga aaaaatcggt gactacgact acaaagttct
    13441 tatgcattat taatgaaaac agtccctagg gctgttttct ctacactagg atataaaaat
    13501 agactataac tagttagaga aaaactataa ttgaaaacta tatcttcttg cagtataata
    13561 aaaggactaa gtgtttgaga tttgtcttca aacacatgca attattcctg agagagtaca
    13621 gttaggagag ggttatgccg attcgaattg ataaaaaatt gccagctgtt gagattttac
    13681 ggacagagaa tatctttgtc atggatgatc aacgtgctgc ccaccaagat atccgtcctt
    13741 tgaagatttt aattttaaat ctcatgccac agaaaatggt cacagagacc cagttgttgc
    13801 gtcacttggc taatacaccc ctacaactgg atattgattt tctctatatg gagagccacc
    13861 gttctaaaac aactcgttca gagcacatgg agaccttcta taaaactttt cctgaagtca
    13921 aggatgagta ttttgatggg atgatcatca cgggtgctcc agttgagcat ttaccatttg
    13981 aggaagtgga ctattgggag gaatttaggc agatgcttga gtggtctaag acacatgtct
    14041 attcgaccct tcatatctgt tggggggctc aggctgggct ttatctgcgc tatggtgtag
    14101 aaaaatacca gatggacagt aagctatcag gtatttatcc tcaggacacc ctaaaagagg
    14161 gtcaccttct atttagaggc tttgatgata gctatgtatc ccctcattca cggcacacgg
    14221 agatttctaa ggaagaggtc ttaaacaaga ccaatctcga gattttatca gaaggacctc
    14281 aggttggggt ttctattttg gccagtcgtg atttacgaga aatttatagt tttggtcatt
    14341 tggagtatga ccgtgatacc ttggcaaaag agtattttcg agatcgtgat gcaggtttcg
    14401 acccacatat tccagaaaat tactttaaag atgatgatgt taatcaggta ccttgtcttt
    14461 gttggtcttc atctgcagcc ctctttttca gtaattgggt aaaccatgcg gtctatcagg
    14521 agacgccttt tgattggaga aagatagaag atgatgcatc tgcatatggg tatttataag
    14581 aggaattatg acatatttag acgcttttaa atcaggtacc ttggttttac cgagtgccct
    14641 gctcttgcat tttaaggaac tctttccttc tagcgacgat tttctggttt ggcaattttt
    14701 ctatttgcaa aatacgacag gcttagaaga aatgtcgcca agccagattg ctgaaaggat
    14761 tggcaaggaa atttcggatg tcaaccagtc catttctaat ctgacggaaa ggggactgct
    14821 ccagtatcgt actatcgaat taaatggcga aattgaattg ctctttgatg ctagtttggc
    14881 cttggaacgt ttggatgacc tgtttggagc agttcgttca agttcagacc agctaacacc
    14941 tcaaaaccag ctcaaggatt tggtggaaac cttccagcag gagttgggac gattgttgac
    15001 gccttttgag attgaggatt tgaccaagac actaaaggaa gatggaacca gtgctgactt
    15061 gattaaggag gctcttcgtg aagctgtttt gaatggaaaa ccaaactgga agtacattca
    15121 ggcgattttg agaaactggc gccatgaagg aatcaagagt gtggctcaaa ttgaggccaa
    15181 gagagcagaa agagaagcaa gcaatcctca gttgacacag gtatctgcag atttcagaaa
    15241 tgccatggat ctctggaagg attaatccat gcaagtaggc ttgaaatccg agtaagattt
    15301 gcaagctgtg tataattgtg atagaataaa tagaaaataa attgaaaaaa gaggtatgtg
    15361 aaatgtcacg taaaccattt atcgctggta actggaaaat gaacaaaaat ccagaagaag
    15421 ctaaagcatt cgttgaagca gttgcatcaa aacttccttc atcagatctt gttgaagcag
    15481 gtatcgctgc tccagctctt gatttgacaa ctgttcttgc tgttgcaaaa ggctcaaacc
    15541 ttaaagttgc tgctcaaaac tgctactttg aaaatgcagg tgctttcact ggtgaaacta
    15601 gcccacaagt tttgaaagaa atcggtactg attacgttgt tatcggtcac tcagaacgcc
    15661 gtgactactt ccatgaaact gacgaagata tcaacaaaaa agcaaaagca atctttgcga
    15721 acggtatgct tccaatcatc tgttgtggtg aatcacttga aacttacgaa gctggtaaag
    15781 ctgctgaatt cgtaggtgct caagtatctg ctgcattggc tggattgact gctgaacaag
    15841 ttgctgcctc agttatcgct tatgagccaa tctgggctat cggtactggt aaatcagctt
    15901 cacaagacga tgcacaaaaa atgtgtaaag ttgttcgtga cgttgtagct gctgactttg
    15961 gtcaagaagt cgcagacaaa gttcgtgttc aatacggtgg ttctgttaaa cctgaaaatg
    16021 ttgcttcata catggcttgc ccagacgttg acggtgccct tgtaggtggt gcgtcacttg
    16081 aagctgaaag cttcttggct ttgcttgact ttgtaaaata atcagtaagt agcaaaagct
    16141 aggtggaaca gcattcagat gtctgttaca ttttttatag gagaggaaag attgaaaaca
    16201 aaaattggat tagcaagtat ctgtttacta ggcttggcaa ctagtcatgt cgctgcaaat
    16261 gaaactgaag tagcaaaaac ttcgcaggat acaacgacag cttcaagtag ttcagagcaa
    16321 aatcagtctt ctaataaaac gcaaacgagc acagaagtac agactaatgc tgctgcccac
    16381 tgggatgggg attattatgt aaaggatgat ggctctaaag ctcaaagtga atggattttt
    16441 gacaactact ataaggcctg gttttatatt aattcagatg gtcgttactc gcagaatgaa
    16501 tggcatggaa attactacct gaaatcaggt ggatatatgg cccaaaacga gtggatctat
    16561 gacagtaatt acaagagttg gttttatctc aagtcagatg gggcttatgc tcatcaagaa
    16621 tggcaattga ttggaaataa gtggtactac ttcaagaagt ggggttacat ggctaaaagc
    16681 caatggcaag gaagttattt cttgaatggt caaggagcta tgatgcaaaa tgaatggctc
    16741 tatgatccag cctattctgc ttatttttat ctaaaatccg atggaactta tgctaaccaa
    16801 gagtggcaaa aagtgggcgg aaaatggtac tatttcaaga agtggggcta tatggctcgg
    16861 aatgagtggc aaggcaacta ctatttgact ggaagtggtg ctatggcgac tgacgaagtg
    16921 attatggatg gtgctcgcta tatctttgcg gcctctggtg agctcaaaga aaagaaagat
    16981 ttgaatgtcg gctgggttca cagagatggt aaacgctatt tctttaataa tagagaagaa
    17041 caagtgggaa ccgaacatgc taagaaaatc attgatatta gtgagcacaa tggtcgtatc
    17101 aatgattgga aaaaggttat tgatgagaac gaggtggatg gtgtcattgt tcgtctaggt
    17161 tatagcggta aagaagacaa ggaattggcg cataacatta aggagttaaa ccgtctggga
    17221 attccttatg gtgtctatct ctatacctat gctgaaaatg agaccgatgc tgagagtgac
    17281 gctaaacaga ccattgaact tataaagaaa tacaatatga acctgtctta ccctatctat
    17341 tatgatgttg agaattggga atatgtaaat aagagcaaga gagctccaag tgatacaggc
    17401 acttgggtta aaatcatcaa caagtacatg gacacgatga agcaggcggg ttatcaaaat
    17461 gtgtatgtct atagctatcg tagtttatta cagacgcgtt taaaacaccc agatatttta
    17521 aaacatgtaa actgggtagc ggcctatacg aatgctttag aatgggaaaa ccctcattat
    17581 tcaggaaaaa aaggttggca atatacctct tctgaataca tgaaaggaat ccaagggcgc
    17641 gtagatgtca gcgtttggta ttaagcgatg atttgaaaga gggatgtgat agtagcaccc
    17701 tctttttctt tgttttatga tagttcatcc tcgagtaaat tcaagttctt gctcggaaat
    17761 gaagcttata tagtagattg aatatagaca aataccttgt gattggtaaa acattttaga
    17821 aattcattta cctttcctaa tcgacttggt ttcatcttat ttcaatctat tatagtattg
    17881 gggaatttct tcaaaccaca tcagcttggt cagttctacc tgcgacctca aaacttgtgc
    17941 tttggtcaag ctgggtttag tttcctagtt tgctgatgga tttccattga ctataagcat
    18001 ccaaccctct ttttgtcttc taaagaattc ttaaattatc agtctattgc aacttttctc
    18061 atataagttc tttgtcttgc tattggtttt ccttagtagt atactaaggt agtaatcatt
    18121 aagaagtggt tacaaaaaat aatgaatgag gtaaagaaaa tggtagaatt gaaaaaagaa
    18181 gcagtaaaag acgtaacatc attgacaaaa gcagcgccag tagcattggc aaaaacaaag
    18241 gaagtcttga accaagctgt tgctgatttg tatgtagctc acgttgcttt gcaccaagtg
    18301 cactggtata tgcatggtcg tggtttcctt gtatggcatc caaaaatgga tgagtacatg
    18361 gaagctcttg acggtcaatt ggatgaaatc agtgaacgct tgattacact cggtggaagc
    18421 ccattctcta cattgacaga gttccttcaa aatagtgaaa tcgaagaaga agctggtgaa
    18481 taccgtaatg ttgaagaaag cttggaacgt gttcttgtta tctaccgtta cttgtcagaa
    18541 cttttccaaa aaggtttgga tgtcactgat gaagaaggtg acgatgtgac aaacggtatc
    18601 tttgcaggcg ctaaaactga aacagataaa acaatttgga tgcttgcagc cgaacttgga
    18661 caagcacctg gtttgtaaga aataaggaaa catagataaa tctgtaggaa atttcttaca
    18721 gattttttct attttgtaag ctattccaaa ccagtctttt tttgagtttt ttgataaaat
    18781 agtactatca gtgaaaagga tggaagcatg actaagaaaa tcgtagctat ttgggcccag
    18841 gatgaagagg gtgtgattgg taaggaaaat cgtctgcctt ggcatttgcc agcagaattg
    18901 cagcacttta aagaaacaac tctgaatcat gctatcttga tggggcgtgt gacctttgat
    18961 gggatggggc gtcgcttgct tccaaaacgg gaaaccttga ttttgacgcg taatccggaa
    19021 gaaaagatag atggggttgc tacttttcag gacgtccagt ctgttcttga ctggtatcag
    19081 gctcaagaaa agaatctcta cattatcggt gggaagcaaa tttttcaggc ttttgaacct
    19141 taccttgatg aagtgattgt cactcacatt catgctcggg tggaaggaga tacctatttc
    19201 cctgaagaac ttgacttgtc tctttttgag acagtttcaa gcaaatttta cgccaaagat
    19261 gagaagaatc cttatgattt taccatccaa taccgcaaga gaaaggaagt ctaatggaac
    19321 ggagtttatt tggtctattc acggcttttc tgtgtttcat ctgctttctg gcaggagcac
    19381 aagcttttcg taaaaagcgt tacgggcttt ctatcctcct ttggttaaat gcctttacca
    19441 atctggtcaa tagtattcat gctttttaca tgaccttatt ttagatagaa tgacagtata
    19501 gaatggaagg aaatcatgtc tacaaataga aaaaatgata tgatggttta ttgctcattt
    19561 tgtggcaaaa accaagaaga agtacaaaaa ataattgctg gcaacaatgc ttttatttgt
    19621 aatgaatgcg tggagttagc tcaggaaatc attcgagaag aattggttga ggaagtcttg
    19681 gcagacttgt ctgaggtgcc aaaaccaatt gaactcctcc atatcttgaa ccactatgta
    19741 attggtcaag atcgtgccaa gcgtgccttg gcagtggcgg tttataacca ctacaaacgc
    19801 atcaatttcc acgatacacg cgaagagtca gaagatgtgg atttgcagaa gtcaaacatt
    19861 ttgatgattg gcccaactgg ttcagggaaa actttccttg cccagacctt ggctaagagc
    19921 ttgaatgtac cttttgctat tgcggatgcg acagctctga cggaggctgg ttatgtgggt
    19981 gaggatgtgg aaaatatact cctcaaactc ttgcaggttg ctgactttaa catcgaacgt
    20041 gcagagcgtg gcattatcta tgtggatgaa attgacaaga ttgccaagaa gagtgagaat
    20101 gtgtctatca cacgtgatgt ttctggtgaa ggggtgcaac aagcccttct caagattatt
    20161 gagggaactg ttgctagcgt accgcctcaa ggtggacgca aacatccaca acaagagatg
    20221 attcaagtgg atacaaaaaa tatcctcttc atcgtgggtg gtgcttttga tggtattgaa
    20281 gaaattgtca aacaacgtct gggtgaaaaa gtcatcggat ttggtcaaaa caataaggcg
    20341 attgacgaaa acagctcata catgcaagaa atcatcgctg aagacattca aaaatttggt
    20401 attatccctg agttgattgg acgcttgcct gtttttgcgg ctcttgagca attgaccgtt
    20461 gatgacttgg ttcgcatctt gaaagagcca agaaatgcct tggtgaaaca ataccaaacc
    20521 ttgctttctt atgatgatgt tgagttggaa tttgacgacg aagcccttca agagattgct
    20581 aataaagcaa tcgaacggaa gacaggggcg cgtggacttc gctccatcat cgaagaaacc
    20641 atgctagatg ttatgtttga ggtgccgagt caggaaaatg tgaaattggt tcgtatcact
    20701 aaagaaactg tcgatggaac ggataaaccg atcctagaaa cagcctagag gtgactatgg
    20761 aacttaatac acacaatgct gaaatcttgc tcagtgcagc taataagtcc cactatccgc
    20821 aggatgaact gccagagatt gccctagcag ggcgttcaaa tgttggtaaa tccagcttta
    20881 tcaacactat gttgaaccgt aagaatctcg cccgtacatc aggaaaacct ggtaaaaccc
    20941 agctcctgaa cttttttaac attgatgaca agatgcgctt tgtggatgtg cctggttatg
    21001 gctatgctcg tgtttctaaa aaggaacgtg aaaagtgggg gtgcatgatt gaggagtact
    21061 taacgactcg ggaaaatctc cgtgcggttg tcagtctagt tgaccttcgt catgacccgt
    21121 cagcagatga tgtgcagatg tacgaatttc tcaagtatta tgagattcca gtcatcattg
    21181 tggcgaccaa ggcggacaag attcctcgtg gtaaatggaa caagcatgaa tcagcaatca
    21241 aaaagaaatt aaactttgac ccaagtgacg atttcatcct cttttcatct gtcagcaagg
    21301 cagggatgga tgaggcttgg gatgcaatct tagaaaaatt gtgaggaaaa gaaaatggca
    21361 aaaacaattc atacagataa ggccccaaag gctatcggac cctatgttca aggaaaaatc
    21421 gttggcaacc ttttgtttgc tagcggtcaa gttcccctat cccctgaaac tggggaaatt
    21481 gtaggagaga atatccaaga acagacagag caagtcttga aaaacatcgg tgctattttg
    21541 gcagaagcag gaacagactt tgaccatgtt gtcaaaacaa cttgcttctt gagcgatatg
    21601 aacgactttg ttccttttaa tgaggtttac caaacggcct tcaaagagga attcccagct
    21661 cgttcagctg tggaggtagc tcgtcttcct cgtgatgtaa aagtcgaaat tgaagtcatc
    21721 gcagagattg gataagctag ttgaagtttg gtgttgccaa acttcttttg atataaggag
    21781 aaaaagatga caaagaaaca acttcacttg gtgattgtga cagggatgag tggcgcaggg
    21841 aaaactgtag ccattcagtc cttcgaggat ctaggttatt tcaccattga taatatgccg
    21901 ccagctctct tgcctaagtt tttgcagctg gttgaaatta aggaagataa tcctaagttg
    21961 gctttggtag tggatatgcg tagccgttct ttcttttcag agattcaagc tgttttggat
    22021 gagttggaaa atcaagatgg tttggatttc aaaatcctct ttttggatgc ggctgataag
    22081 gaattggtcg ctcgttacaa ggaaaccaga cggagtcacc cactagcagc agacggtcgt
    22141 attttagatg gaatcaagtt ggaacgtgaa ctcttggcac ctttgaaaaa tatgagccaa
    22201 aatgtggtgg atacgactga actcactcca cgtgaactgc gcaaaaccct tgcagagcag
    22261 ttttcagacc aagaacaagc ccagtctttc cgtatcgaag tcatgtcttt cggatttaag
    22321 tatggaatcc cgattgatgc ggacttggtc tttgatgtcc gtttcttgcc aaatccctat
    22381 tatctaccag aactgagaaa ccaaacgggt gtggatgaac ctgtttatga ttatgtcatg
    22441 aaccatcctg agtcagaaga cttttatcaa catttattgg ccttgattga gccgattctg
    22501 ccaagttacc aaaaggaagg taagtccgtt ttgaccattg ctatgggatg tacgggtgga
    22561 caacaccgta gtgtggcatt tgctaaacgc ttggcgcagg acttatccaa gaattggtct
    22621 gttaatgaag ggcatcgcga caaagaccgc agaaaggaaa cggtaaaccg ttcatgagaa
    22681 aaccaaagat aacggtgatt ggtggaggga ctggaattcc cgtcattcta aaaagtctgc
    22741 gggaaaaaga tgtggaaatc gcagctatcg tgacggtggc agatgatggt ggttcttcag
    22801 gtgaactccg aaaaaatatg caacagttga caccgccagg tgatcttcgt aatgtccttg
    22861 tggccatgtc ggatatgcct aagttttatg agaaggtctt tcagtatcgg ttctctgagg
    22921 atgccggagc ctttgctggc catccattgg gaaatctcat cattgctggc ttgtcagaaa
    22981 tgcagggttc aacctataat gccatgcagt tattgagcaa atttttccat acaacaggga
    23041 aaatttatcc ttccagtgac catcctttga cccttcatgc agtctttcag gatgggacag
    23101 aagtggctgg agagagtcat attgtagacc atcgaggcat aattgacaat gtctatgtga
    23161 ccaatgccct aaacgatgat acgcctctgg ccagccgtcg agtagtgcag accatccttg
    23221 aaagtgacat gattgtccta gggccaggtt ccctctttac ctctattttg cccaatatcg
    23281 tgattaagga aattgggcgg gctcttttgg aaactaaggc agaaattgcc tatgtctgca
    23341 atatcatgac ccaacgtggg gagacggaac actttacaga tagcgaccac gtggaagtct
    23401 tgcatcgtca ccttggtcgc ccttttatcg acactgtctt ggtgaatatt gaaaaagtgc
    23461 ctcaggaata catgaattcc aaccgttttg atgaatactt ggtgcaagtg gaacacgatt
    23521 ttgtaggtct ttgtaagcaa gtttcgcgcg tgatttcatc taacttcctt cgtctggaaa
    23581 atggcggtgc cttccacgat ggagatttga ttgtggacga gttgatgcgc attatacagg
    23641 tgaaaaaatg agtttcacag tagcagtaaa agaagaaatc ctgggccaac accatctgag
    23701 ccggcatgaa ttatctgcca ttatcaaaat gtctggtagc atcggtctct cgacttcggg
    23761 cttgactttg tcagttgtga cagaaaatgc caaactggcc cgtcacctct atgagtcctt
    23821 tctccatttc tatgaaatca aatctgaaat tcgtcaccac caacggagca atcttcgcaa
    23881 gaatcgcgtc tataccgttt ttacagatga aaaggtgcag gatttgttaa gtgatttgca
    23941 cttggcagac tctttctttg gcctggaaac aggtattgat gaggcgattt tatcggatga
    24001 ggaagcaggt cgtgcctatc tctgtggcgc tttcttggca aatgggagca ttcgtgaccc
    24061 tgagtcaggc aagtaccagt tggaaatcag ttctgtttat ctggaccacg cgcaaggaat
    24121 tgcctccctt ctccaacagt ttttactgga tgccaaggtg cttgagcgca agaagggggc
    24181 tgtgacctat ctccagcgag cagaagacat tatggacttc ttgatagtaa tcggagccat
    24241 gcaggcacgt gatgattttg agcgggttaa gattttgcga gaaacccgta acgacctcaa
    24301 tcgggctaat aatgccgaga cagctaatat cgctcggaca gtttctgcca gcatgaagac
    24361 cattaacaat atcagtaaaa taaaagatat catgggctta gaaaatttgc cagtagattt
    24421 gcaggaagtg gcgcaactga ggattcagca cccagactac tctatccagc agttagcaga
    24481 tagtctcagc acccctctga ccaaaagtgg tgtcaatcac agactcagaa aaatcaataa
    24541 gatagcggat gaattataaa accacgaatc ctatgtgact cgtggttctt ttttataaac
    24601 tggtagagtg ttttggttgt actttttgtt cagggtcaat gtagttgata gcgttgttga
    24661 cagcagttgg agcttctccg aggcctgtcg caatcagatc aatttttccg tcatagtagc
    24721 agcagtcacc gatagcatag atacctgctt ggctggattc ctgtttgctg ttgacgataa
    24781 tcttgtgacg gttgaggtcg agcccccagt ttttaaggtt accgacagaa gatttgaaac
    24841 catagttgac aaagaggtgg tctaggtcaa tggtttcagt ttcatcagat ttgacttttg
    24901 tgatttcaag tttatcaagt gtttttccat ttccaaggag ttggctaggg gcgaatggtg
    24961 tcttgatggt tacagatgat tcttgcaagg cttgaacact gtgttccaag gcacggaaat
    25021 tatctctgcg gtgaacaagg gtagttggtg cgattttttc aaaagccaaa gtccaatcca
    25081 cagccgagtc tcccccacca agaatcgtca cttttttacc agcgtattgc tgaatgttag
    25141 aaacgtggta gtggatattt tcatagccct caaccccttc aagttccagc ggacgtggtt
    25201 tgaaggcacc gccacccata gcgatgataa ctgttttagt caggtgactt cctttagaag
    25261 ttgtgatggc aaattcttct tcttgtttgt caatctcaag aaccgtttca ttgagatgaa
    25321 taggggtatc aaatccattt agctgttcaa tcaagcggtt agtcaactct tctccagtca
    25381 ggtttgggaa gcctggtacg tctaggattt ccttttcagg gtagagaata gcaggttgtc
    25441 cacctagctg gggaagagag tcgatgattt gaaccttggc ttggcgtagg tgggcataaa
    25501 aggctgcaaa aagcccgaca ggaccaccac ccacaatggt aatatcatag agttgagaca
    25561 tagtttctcc tttgtttttt ctagtcagtt tattttatca tatttttgtt caatgtaaaa
    25621 tggactagga tagggaaaaa aatgccagaa aatggtataa taggacgaaa gtgttttgat
    25681 agggaggata tcagggatga attttcaaca attatccaat ttgcaatatt ggaccagttt
    25741 gtttgcaagt ccatggacga tagctatcaa tctgattgat attttgattg ttgcttatat
    25801 tttataccat tttacaaaag ctattgcagg aaccaagatt atgattttgg tacgtggagt
    25861 tttggtattt attttagctc aaatccttgc aaatatgatt ggtttgacta cgatttcttg
    25921 gttaatcaat caaattatta cttatggggt tattgcggcg gttgttatct tctctccaga
    25981 gattcggact ggtttggaac gtttgggaag agcgacagat ttcttttcca atgcccctat
    26041 tagtgctgag gaacagatga ttcgtgcctt tgttaagtct gttgaataca tgagtcctcg
    26101 taaaatcggg gccttggttg ctattcagcg tgtacgtacc ttgcaggagt atatttcgac
    26161 aggaattccc ttggatgcta agatttctgc agaacttctc attaacattt ttattcccaa
    26221 cactccccta catgacggtg cggtgattat caaagaagaa cgtatcgctg tgacgtctgc
    26281 ctatctgccc ttgacaaaaa acacagggat ttccaaggaa tttgggacca gacaccgggc
    26341 ggctatcggt ttatcagaag tctcagatgc cttgactttt gtcgtatcag aggaaacggg
    26401 aggaatttcg ataacctata atggaaggtt taagcacaac ctaacacttg atgaatttga
    26461 aacagaatta cgtgaaatct tacttccaaa agaggaagta ggtcttagtt ttaaagaacg
    26521 attgctagga ggatggaaac atgaaaaaaa atagtttata tatcatatcc tcactctttt
    26581 ttgcttgtgt cttatttgtc tatgctacgg cgacgaattt tcaaaacagt accagtgcta
    26641 ggcaggtaaa aacggaaacc tatactaata cagtaacaaa tgtccctatt gacatacgct
    26701 ataatagtga taagtatttt attagcggtt ttgcttcaga agtatcagtg gtcttgactg
    26761 gtgcaaatcg cctatcgcta gctagtgaaa tgcaagaaag tacacgtaaa ttcaaggtta
    26821 ctgctgacct aacagatgcc ggtgttggaa caattgaagt tcctttgagc attgaagatt
    26881 tacccaatgg gctgaccgct gtggcgactc cgcaaaaaat tacagtcaag attggtaaga
    26941 aggctcagaa ggataaggta aagattgtac cagagattga ccctagtcaa attgatagtc
    27001 gggtacaaat tgaaaatgtc atggtgtcag ataaagaagt gtcgattacg agtgaccaag
    27061 agacattgga tagaattgat aagattatcg ctgtcttgcc aactagcgaa cgcataacag
    27121 gtaattacag tggttcagta cctttgcagg caatcgaccg caatggtgtt gtcttaccgg
    27181 cagttatcac tccgtttgat acaataatga aggtgactac aaaaccagta gcaccaagtt
    27241 caagcacatc aaattcaagt acaagcagtt catcggagac atcttcgtca acgaaagcaa
    27301 ctagttcaaa aacgaattaa aaatagaaaa ggattttata acaatgggta aatattttgg
    27361 gactgatgga gtccgtggag aagctaacct agaactaaca ccagaattag cctttaaact
    27421 aggacgtttt ggaggctatg ttcttagtca acatgaaacg gaagcgccga aagtctttgt
    27481 aggacgtgac acacgtattt caggggaaat gctagaatcg gccttggtgg caggtctcct
    27541 ttcagtaggg attcacgtat acaaacttgg tgtccttgca acaccagcag tagcttactt
    27601 ggttgaaact gaaggagcaa gtgctggtgt catgatttct gctagccaca atccagctct
    27661 tgataacgga atcaagttct ttggcggtga tggcttcaaa ctagatgatg aaaaagaagc
    27721 agaaattgaa gccttgctag atgctgagga agacactctt cctcgtccaa gtgcagaagg
    27781 cttaggaatt ttggtagatt atccagaagg cttgcgtaag tatgaaggat accttgtttc
    27841 aactggaact cctcttgatg gaatgaaggt tgccttggat acagctaatg gagcagcttc
    27901 taccagtgcc cgtcaaatct ttgcagacct tggtgcccaa ttgacggtta tcggggaaac
    27961 accagacggt cttaacatca accttaatgt tggttcaaca catccagaag cccttcaaga
    28021 agtggtcaaa gaaagtgggt cagctattgg tttggccttt gatggagaca gtgaccgctt
    28081 gattgctgtt gatgagaatg gtgacatcgt cgatggtgac aagattatgt acatcatcgg
    28141 aaaatacctt tctgaaaaag gacaattggc tcaaaataca attgtgacaa ctgttatgtc
    28201 taaccttggt ttccacaagg ccttgaatcg cgaaggtatt aacaaggcag ttactgcagt
    28261 tggtgaccgc tacgttgttg aagaaatgag aaaatcaggt tacaaccttg gtggtgaaca
    28321 gtctggtcac gttatcttga tggattacaa taccacaggt gatggtcaat tatcagcaat
    28381 tcaattgact aaaatcatga aggaaactgg taagagctta tcagagttgg cggcagaagt
    28441 aacgatttat ccacaaaaat tagttaatat ccgagtggaa aacgtcatga aggaaaaggc
    28501 catggaagtg ccagctatca aggccatcat cgagaagatg gaagaagaaa tggcggggaa
    28561 cggccgtatc cttgttcgtc caagtggaac agagcccctc ttgcgtgtta tggcagaagc
    28621 gcctacaaca gaagaagtgg actactatgt tgatactatc acagatgtag ttcgtgctga
    28681 aattgggatt gactaaatcc tagaaaacta gatgaaaata aaaaattatg gtacaatagc
    28741 ttataatatt gtagccgatg gagaaagaca tgaatcttaa acgtgaacaa gaatttgtta
    28801 gtcagtatca ttttgatgca cgtaattttg aatgggaaaa tgaaaatgga gctcctgaaa
    28861 ccaaggtgga tgtgaatttc caattgctcc aacatgacca agaaaatcaa gtgacttcct
    28921 tgattgtcat tttgagcttt atgattgtat ttgataagtt tgtcattagt ggaacaatct
    28981 cacaagtaaa ccatatcgat ggccgtattg tcaatgaacc aagtgaattg aaccaagaag
    29041 aggtggaaac cttggctcgt ccatgtttga acatgctcaa tcgtttgact tacgaagtga
    29101 ctgaaattgc attagactta ccaggaatta atttggagtt ctaatatgaa attagctgtt
    29161 ttcacagatt cctctgctta tctcagtgaa gagaccttgc aaagagaaga cttgtttgtc
    29221 ttggatattc ctgtcaatat tgatggtgag gaatatgtcg aaggcatcaa tctgtctgct
    29281 gaggaatttt accaaaaaat ggctcaggct tctgaattgc ctaagaccag tcaaccaagt
    29341 attgccaagt tagatgagat cttaacttcg ctcaaagaac aaggctatac acatgccttg
    29401 gggcttttcc tatcttctgg aatttcaggt ttttaccaaa atatccagta tatggtcgat
    29461 gactatgagg gcttaaccat tgctttccca gacactttga ttacaagtgc tcccctaggc
    29521 atcatggttg aaagcgtctt taattggcgt gatcagggcg atgattttgc cagcattcag
    29581 gataagctag ccattcaaat cagccgtacg tcagccttta tcatggtaga tgacttggat
    29641 catttggtaa aaggtggacg cctttcaaat ggggctgcca ttttgggcaa tttgcttagc
    29701 attaagccaa tcctttattt taacgatcaa ggtgtgattg aagtttacga aaaagttcgt
    29761 actgaaaaga aggccaccaa gcgcttaatt gaaattatca aggaaacaac ggcttcaggt
    29821 caataccggg tcattgtcat tcatgggaat gctcctgaaa aggctgaaga attgcgtcag
    29881 cacttgcttg attttggctt gggttcggat gtttcacttg ctacatttgg tagtgtcatt
    29941 ggaacgcacc taggagcagg aagtattgct ctgggttata ttccagtgat ttagttggcg
    30001 cttctaggct agttaagatg ccttgtatct tagaaattga acacggacta cctgcctctt
    30061 gaaaaaagat gcctgtcttg cctttcgtgg aaagtcagtg ccattcccta tttttcagag
    30121 gcagctaagg tcctttgtat cttgataatt gaacacgcct ggaaccctgt gtgaaaaaga
    30181 tagttcttcc aagaagtaga cacttcttga ttggaattcc tattttcact ttgtgttctt
    30241 acggactttg tatcttagac aggagtagag atgagtattc gagtaattat tgccggtttt
    30301 aagggaaaga tgggccaggc tgcttgtcag atggtattga ctgatccaga cttggacttg
    30361 gtggcagttt tggatccttt tgagtctgag tcagaatggc agggtattcc tgttttcaag
    30421 gataaggctg atttagctgg ttttgaagcg gatgtctggg tagattttac tactccagct
    30481 gttgcctacg aaaatacacg ttttgctctt gaaaatggct ttgctccagt agttggaacg
    30541 actggtttca cgagtgaaga aattgcagag ctaaaagaat tttctcgtgc ccaagacttg
    30601 ggtggcctga ttgcccctaa ctttgccttg ggtgctgtct tactcatgca atttgcgacg
    30661 caggctgcca aatatttccc aaatgtggag attattgagc tccatcatga caagaaaaag
    30721 gatgctccga gtggaacagc cattaaaaca gctgagttga tggcagaggt tcgagagtcc
    30781 attcagcaag gcgcagcaga tgaggaagag ctgattgctg gtgctcgtgg tgctgacttt
    30841 gatgatatgc gcatccactc agttcgtttg ccaggcttgg tagctcatca ggaagtcatc
    30901 tttggcaatc agggagaagg gttgaccctc cgtcatgact cctatgatcg catctccttc
    30961 atgacaggag tcaatttggg aattaaagaa gttgtcaagc gtcatgagct tgtctatgga
    31021 ttagaacact tattatgaga ttaacgcaaa tgccttctga atttcagaag gctttaccag
    31081 tattagaaaa aattaaagaa gcaggctttg aggcttattt tgttgggggc tctgttcgag
    31141 atgcccttct ccatagccct atccacgatg tggatattgc gacgtcttct tatccagaag
    31201 agaccaagca gatttttccg cgaacagccg atatcggaat cgagcatgga accgtcttgg
    31261 tcttagatgg ggatgaggag tatgaggtaa caacctttcg gacagaggat gtctatgtgg
    31321 actatcgcag acccagtgcg gtttcctttg tacgatcgct agaagaagac ctcaaacgcc
    31381 gtgatttcac agtcaacgcc tttgccttgg atgagacagg agaaatcgtt gacttgttcc
    31441 atggtttaga agatttggaa aagcaagtct tgcgagcagt tggagtggct agtgagcgtt
    31501 tcaacgaaga tgctttacgg attatgcgtg gtttccgttt tcaggctagt cttggttttg
    31561 cacttgagcc agaaacattt aaagctatga agaccttgac gccgcttttg gagaaaattt
    31621 ctgtagagcg taccttcgtc gagtttgata aactcttgct ggctccattt tggagaaggg
    31681 gcttggcttc catgattgag agtcaagcat atgactatct ccctgatatg gcatctagcc
    31741 aggacaagct caacagactg tttgatttgg agactgattt tacttttgaa tcctctgaac
    31801 aagcctgggc ggctttacta tgggctttgg agattgaaaa tgcgcagtca tttttgaaat
    31861 cttggaagac ctcacgccag tttgccaagc aagttcagga tttgctgatt attttggctc
    31921 tgcgtgaaaa tggagaattg agtaagcgag attgttatcg ctttgacata gatttgcttt
    31981 tacaggctga aaatcttcgt caagctcaag gaaaagaagt caacccacaa gccatcacag
    32041 aaaaatacca aagcttgact atccatgaca agaaagagat tcagattaat ggcgatattt
    32101 tgatcaagga atatggctat cagccaggcc cagacttggg agagatttta acagagattg
    32161 agtttgccat tgtcgatgga gaattggaga ataatcgtga agccatccat gcttacctga
    32221 gggagaaaaa atgagtgatt ttatcgttga aaaactaagt aaatccgttg gtgacaagac
    32281 cgtttttagg gatatttcct ttattatcca tgacttagac agaattggtt taatcggtgt
    32341 caatgggact ggcaagacca cccttttgga cgtcctttct ggtgtttctg gatttgatgg
    32401 ggatgtcagt cctttttcag ctaaaaatga ttaccagatt ggttacttga ctcaggatcc
    32461 tgattttgat gatagaaaga cagttttgga tacggttcta tctagtgaac tcaaggaaat
    32521 ccagctcatt cgtgagtatg aattgattat gctcgactat agtgaggaca agcaggcgcg
    32581 tttggaacgt gtcatggcag agatggactc tctccaagct tgggaaatcg aaagtcaggt
    32641 caagaccgtt cttagcaaat tgggcattca agacttatct actcctgttg gggaattgtc
    32701 aggtggtctg agaagacggg tacagttggc acaagtctta cttggcaacc acgacctctt
    32761 gcttttggat gagccgacca accatctgga tattgcgatt attgagtggc tgaccctctt
    32821 tttgaaaaat tctaagaaga ccgtcctttt tatcactcac gatcgttatt tcttagacgc
    32881 tttgtcaaca cggattttcg agttggatcg tgcaggcttg accgagtacc agggcaatta
    32941 ccaggactat gttcgcctaa aggcggaaca ggatgagcgc gacgcggctc ttcttcacaa
    33001 aaaagaacaa ctctacaaac aagaactggc ctggatgcgc agacaaccgc aggcgcgtgc
    33061 gaccaagcag caggcccgta tcaatcgatt ccatgatctg aaaaaggaag tttcaggcag
    33121 tagtgctgag acagacttga ccatgaactt tgaaaccagt cggattggga agaaagtcat
    33181 cgagtttcag gctgtttcct ttgcctatga aaacaagcct attttgcaag attttaatct
    33241 cttggtgcag gccaaagacc gtatcggaat cgttggggac aatggtgttg gaaaatcaac
    33301 cctgctcaac ttgattgcag gaagtcttga gccaactaaa ggacaagttg tgattgggga
    33361 aactgttcgc atcgcctatt tctctcagca aattgagggt ttggatgaaa gcaagagagt
    33421 tatcaattac ctgcaggaag tggcagaaga ggtcaagacc agtggcggtt ctacgacttc
    33481 gattgcagag ttgctggagc agttcctttt cccacgttcg acgcatggaa ccttgattga
    33541 gaaattgtca gggggtgaga aaaaacgtct ttatctcctc aaactgcttt tggaaaaacc
    33601 aaatgttctt cttttagacg agccaaccaa tgacctagat attgcaactt tgacagtctt
    33661 agagaatttc ttgcaaggtt ttgcaggtcc cgttttaaca gtcagtcacg accgctattt
    33721 cttggataag gtagcgacca agattctcgc ttttgaggat agcaagattc gtcctttctt
    33781 tggtcattac accgactatc ttgatgaaaa agcttttgaa acagatatgg ccaatcaagt
    33841 gcaaaaggtc gaaaaggaaa aagtggtcaa ggttcgagaa gacaagaaac gtatgaccta
    33901 ccaagaaaag caggagtggg caagtattga aggtgatatt gaaaccttgg aaaaacgtat
    33961 cgctgctatt gaagaggaaa tgcaggctaa cggctctgac tttggtaagc tggctactct
    34021 ccaaaaagaa ttggatgaga aaaatgaagc actccttgaa aaatacgaac gctatgagta
    34081 tctcagtgaa tttgatagtt aagactattc gaaaatcaaa gtgcaaacta tgtcggcttc
    34141 acattgccga ctcaagtaca gcctgtgacc ggcttcctaa tttgcacttt gatttttatt
    34201 gagtataaaa gaatagaaaa atcccgtctc atgaaaggat ttctctattc ttttttggtt
    34261 tcttgatgaa agatattttg ccaggtttcg tggcgacgcc agatactggt atggatgata
    34321 ccatctaact catagcagat gagtttggtt ttttgactga tggaagtaat ttgaatgtcc
    34381 ttgatagcag aatttaactc ttttttggtc ttataagcct ctttatccat ctgctctcca
    34441 tcttggcgaa tatagacaaa atcatcagtc aagagttctt ctagttggtt tccttggtca
    34501 atcaattgtt cacgcataag caatttttta tagacattgt ctaaaatttc atcctcagag
    34561 ataggtgctg gttcgatatg gacatcggta tcaaagacgc caaaacgctc ctccagcata
    34621 gactcgacct gatccgcgat ttcatggctt tcaaaaacag acaagtcagg attcatctct
    34681 agtgtaatat ccaggtagat gttgctaccg taggtgcgac ctctttgcga tttgaccttg
    34741 ctgattttgg gaatttccat gatagccttt tggtagtcct cgagcaggcg gtcgtcaaag
    34801 ccatctgaaa gactaaagga agactcgatg aagatatcat aggcagtctt caagataaag
    34861 aaagtgatga tgatagcaac cagtttatcc acaatcggat aattgaaact actagctagg
    34921 atggcaatgg cggttccaag tgaggtaaca gcgtcagaaa gattgtcctt agcagctgcc
    34981 ttcagcgcct tggagttgga tttcttactg aggcgagtat tgtagagata gaccacaaac
    35041 ataatcgctg cagaaatgat tcctagagtt gcaccaagag gatcaatgac cgtttcttcc
    35101 cgactgagaa tcttttgaat ggtatctctt agaacatcga aaccgacata gaacatgatg
    35161 atagaagtga tcaagcttgc caaatcttca atcttccaat gaccaaaacg gtggtcacgg
    35221 tctgcaggct ggcgcgccat ccgaatcccg attaagaggg ccacatttcc aatgatgtcc
    35281 gatacgttat taaaaccatc ggccaccaaa ctggatgaat gaaggagatg accagctgct
    35341 aatttggctg cagacaagat caaataggtc gaaatactga taatggctcc acgctcagct
    35401 aacttgagat ttgagataga ttgcttcatt caacttcctt tctagtcaca tagcattcta
    35461 ccattatact ggttttcaag gaaaaattca aataaggcag tttttactct ttgaaaactc
    35521 cccaaaattt ggtataatgg tactactcaa gggagtagct ggcagaaacc tgtgatagtg
    35581 tcgtcattcc gaattttatg ctgaaaagta tgctttccgg ccctatctta aacagcgaga
    35641 cttgttatga ttaacaggtc tctttttgtt tgtcgtgaaa agatatgaca gaaagagagt
    35701 ctgttttgca cacaatgtca aggaggagac acatgtcaaa agaacaaaaa cgccaagcgt
    35761 tttatactca gagccctgaa gaggtcttgc aggctgtgga tgcgaccgag caaggtttgt
    35821 catcaagtga ggcggaaaag cgccttgccg aatttgggca caatgaactc gaagaaggcg
    35881 agaaacgatc aatcttggtc aaattcatcg agcaatttaa ggatttgatg attatcatcc
    35941 tagttgcggc agcaatcttg tcagtcgtga cttctggtgg ggaagatatc gcagatgcca
    36001 ttatcatcct agctgtggtt atcatcaacg ctgcctttgg tgtttaccaa gaaggaaaag
    36061 ctgaagaagc tattgaagcc ctcaaatcca tgtctagtcc agttgcccgc gttcttcgtg
    36121 atggacacat ggcagagatt gactctaaag aattggtacc aggcgatatc gttgcccttg
    36181 aagcaggtga cgtggtacca gcggacctac gtttgataga agccaactct cttaaaattg
    36241 aagaagcagc cttgacaggt gaatctgtac cagtcgaaaa agacttgtca gtcgagcttg
    36301 cgacagatgc tggtattggt gaccgtgtca acatggcctt ccaaaactca aacgtaacct
    36361 atggtcgtgg gatgggtgtt gttgtcaata caggtatgta cactgaagtt ggtcatattg
    36421 ctggtatgct tcaagatgcg gatgagactg atacaccact caaacaaaat ttgaacaacc
    36481 tttctaaggt cttgacctat gctatcttgg tcattgccct tgttactttt gtagtgggtg
    36541 tcttcattca agggaaaaat ccacttggtg agttgttgac ttctgttgcc cttgccgttg
    36601 cagccattcc agaaggactt cctgctatcg ttaccatcgt tctttctctt ggtactcaag
    36661 ttttggccaa acgacattcc atcgttcgta agttgccagc agttgaaaca cttggttcaa
    36721 ctgaaatcat cgcttctgat aagactggta cgctgactat gaacaagatg acagtcgaaa
    36781 aagtcttcta cgatgcggtt ctacatgact cagctgatga tattgaacta ggtcttgaaa
    36841 tgccactact tcgttcagtt gtcttggcca atgatacgaa aatcgatgtg gaaggtaact
    36901 tgattggtga cccaaccgaa acagccttta tccaatatgc cttggacaag ggctatgatg
    36961 tcaaaggttt cttagagaaa tatcctcgtg tagctgaatt gccatttgac tctgaccgta
    37021 agctcatgtc aacagttcac ccattgccag atggtcgttt ccttgtagca gtcaagggtg
    37081 cgccagacca actcttaaaa cgttgtcttc ttcgtgataa ggctggggat attgctccga
    37141 ttgatgagaa ggttacaaat ctcattcata caaacaattc tgaaatggct catcaagcct
    37201 tgcgtgtcct tgcaggtgct tataagatta tcgatagtat tccagaaaat ctcacttctg
    37261 aagagcttga aaatgattta atttttactg gtttgattgg gatgattgac cctgaacgtc
    37321 ctgaagccgc tgaggctgtt cgtgtggcta aggaagcggg aatccgtcca attatgatta
    37381 caggtgacca ccaagacaca gcggaagcca ttgccaaacg tttgggaatc attgacgcaa
    37441 acgatacaga aggtcacgtt ttaactggtg ctgaactcaa tgaactgtca gatgaagaat
    37501 ttgaaaaagt cgttggtcaa tactctgttt atgcccgtgt gtctccagaa cacaaggttc
    37561 gtatcgtcaa ggcttggcaa aaacaaggta aagtcgttgc catgacaggt gacggtgtca
    37621 atgacgcgcc agctctgaaa acagccgata tcggtatcgg tatgggaatc actggtacag
    37681 aggtttctaa gggggcttct gatatgattc ttgcagatga taactttgcg actattatcg
    37741 tcgcagtgga agaaggacgt aaggtcttct caaacattca aaagactatt cagtacctac
    37801 tttctgctaa tactgctgaa gtattaacca tcttcctatc aaccttgttt ggttgggatg
    37861 tcttacagcc ggttcatctt ttgtggatta acttggtaac ggataccttc ccagctatcg
    37921 ctcttggtgt tgaacctgcg gaacctggtg tcatgaatca taaaccacgt ggacgcaagg
    37981 caagcttctt ctcaggtggt gttttgagtt ctatcattta tcaaggtgta ctccaagcag
    38041 ctcttgttat gagtgtttat ggccttgcga ttgcttaccc agttcatgtg ggtgacaatc
    38101 atgctattca tgcagatgcc ctaacgatgg cctttgcaac ccttggtttg attcagctct
    38161 tccatgccta caatgtcaag tctgtctacc aatccatctt gacagttggc ccattcaagt
    38221 ctaagacctt taactggtcc atcttggtat cctttatcct tctcatggca acaatcgttg
    38281 tagaaccgct tgaaggaatc ttccacgtaa ccaaactaga cttgtcacaa tggggaattg
    38341 ttatggctgg aagcttctca atgattatca tcgtcgaaat cgttaagttt attcaacgca
    38401 aactcggttt tgacaagaat gcgatttaag ggaggtttcg gatgtcagca tatcaattac
    38461 cgaccgtatg gcaggatgaa gctagtaatc aaggagcttt tacggggcta aacagaccaa
    38521 cagcaggtgc ccgtttcgaa caaaacttgc caaaaggaga acaagctttt cagctttatt
    38581 cactgggaac accaaatggt gtgaaggtta ctatcttatt ggaagaatta ctagaagctg
    38641 gttttaagga agcggcttac gacttgtata agattgctat catggatggg gatcaattcg
    38701 gctcagactt tgtgaagctc aatccaaatt ccaagattcc agccttattg gaccagtcag
    38761 gtactgaaaa cgtaagagtc tttgagtctg ctcatattct tctttacctt gctgagaaat
    38821 ttggagcctt tttaccaagt aatcctgtgg aaaaggtaga agttttgaat tggctattct
    38881 ggcaagcagg tgcagcacct tttctaggtg ggggatttgg acatttcttc aattatgctc
    38941 ctgaaaaatt ggaatatcct attaaccgtt ttacgatgga agtgaaacgc cagttggatt
    39001 tattggataa ggaattggct cagaaacctt atattgcagg caatgactat acgattgcag
    39061 atattgctat ctggtcttgg tatggacagt tagttcaagg aaatctttac caaggttctg
    39121 caaaattctt ggatgcctca agttatcaaa atctagtaaa atgggcagaa aaaattgcca
    39181 atcgtccagc tgttaagcgt ggcttggaag taacttatac agaaattaaa tagcaaaaaa
    39241 gtcatcttcg gatggctttt tgctacagtt gagggaaagg gtttgcagtt atcggctttt
    39301 gtgctataat tgctataata tagtaaaaag caagaggagt gtgaggaagt ggaaaagaaa
    39361 attgtgaagg atatcttatt tttatctcaa gtgtctcatc cggcaagtca ggaggacctt
    39421 tatcttgcca gagatttgca ggatacactc ttagcaaatc gtgatacctg tgttggtcta
    39481 gctgccaata tgattgggat gcagaagcgc gtgattatct ttaatcttgg cttggttccc
    39541 gtggtcatgt ttaacccagt gcttctgtcc tttgaaggat cctatgaggc agaagaaggc
    39601 tgtttgtcct tggtaggtgt gagatcaact aagcgttatg aaaccataag gcttgcctat
    39661 cgtgacagca agtggcagga acagaccatt accttgacag gcttcccagc tcagatttgc
    39721 cagcatgagc tggatcactt ggaaggacga atcatttagg aggaaagcaa atgaaacgaa
    39781 tagtctttga acttattttt atcgcaacga cctggtatat ctttttaccg ccccttaacc
    39841 tgaccagctg ggaatttctc ttcttcctct gtgggcattt gttagttgtg gcaatcttat
    39901 ttggctttgg caaggggata aaccttgtca aaacggttca tgtgcgccac ggtaaggcgg
    39961 aagctgcctt aaatcttgag ggtttcaaaa tcaatcggtt agggaaaatt ctgttagctt
    40021 cgattggagg aattcttctc ttggcagctt tggtttcctt ggtaacttct agcatgtttc
    40081 aggctaaaaa ttatgccaat gtagtcacgg ttacggaaaa agactttact gaatttccta
    40141 agagtgacac cagtaaggtt cctatcctag atagaagtac tgctgaaaaa attggagacc
    40201 gctacttggg ttccctaacc gataaggtgt cgcaatacgt agcggcagat acctataccc
    40261 aattgacaat tgatgggaaa ccttatcggg tcacaccact agaatatgca gaccctatca
    40321 aatggtttaa caatcaagcc aagggaatcg gtgagtatat caaggtggac atggtaactg
    40381 gaaatgcgga tttggtggac ttgaagacac caatcaagta ttcagactcg gagtatttta
    40441 accgtgatgt caaacgtcac ctgcgcttga agtacccgac caaaatcttt aaaactccat
    40501 cttttgaggt ggacgatgag ggcaatcctt tctatgtagc aacggtttac caaaagcaat
    40561 ttggacttgc tgttcctcgt cctgcttcag tcattatctt ggatgctaca aatggagaaa
    40621 ccaaggaata cagcttatca gatgttccag aatgggtgga caggatctat ccagcagagg
    40681 aaaccattga gcaaatcaac tacaacggca agtacaagga cggtttcttg aatgccatga
    40741 tttccaagaa aaacgtgacc cagacgacca atggctataa ttacttgtct atcggtaatg
    40801 acatctatct ctacacaggt gtgacgtcgg ctaatgcgga tgagagtaat cttggtttca
    40861 tccttgaaaa tatgcgaaca ggagaaatta ctaagtatag cttggcttct gcgacagaag
    40921 aatcagcccg tgaatcagca gaaggtgctg ttcaggagaa atcctacaaa gcaaccttcc
    40981 caatcctcat caacctcaat gacaagcctc tctacatcat gggcttaaag gacaatgctg
    41041 gcttggtcaa agagtacgcc ctggtagacg cagtcgagta ccaaaatgtt atcgttgcta
    41101 ctacagtgga agagatgctc agcaagtatg ccaataaaaa cgaccttgaa attgacaatg
    41161 caacgacaga aagcatcaag ggagtagtag cagacctcaa atcagctgtt atcaagggag
    41221 acactgtcta cttctttaaa gttgatggca agatatacaa ggtaaaggct tcagtatccg
    41281 atgaccttcc ttaccttgaa aatggtaaaa ccttcgaagg tcaagtagga aaagacaatt
    41341 atctcaagac ctttaaagta caataatact cttcgaaaat ctctagctct atctgcaacc
    41401 tcaaaacagt gttttgagca gcctgcggct agtttcctag tttgctcttt gattttcatt
    41461 gagtataaaa aaggtttatt taataaatat atgttataat aaggtaaatt aagcctaatg
    41521 gaggacaaag aaatgggttt ttatttgatg gttgcttcaa tgctactcgg actgctcgct
    41581 ttgaagatag gtttttctca gttcaaggag aaaaaagata aattcttatc tatcttaaca
    41641 agtttagctg gcacagccct tgttctcgtg gctgtctggt taggatggcc caaataaata
    41701 gaaaaacctc ggtcaaaccg aggtttttca aatgaatttc ttggttgtgg ctaaaaaata
    41761 tgctacacta tcaatatgaa aattttaatc ccaacagcaa aagaaatgaa cacagacttg
    41821 ccaagtatcg aggcaattcc tttaaaacca gaaagtcaga ccgtgcttga tgccttggct
    41881 ctctattctg ccagtcaatt ggagagtttc tacaaggtat cagctgagaa agcggcggaa
    41941 gaatttcaaa atatccaagc tttgaaaagg caaactgctc aacactatcc agccttgaaa
    42001 ctttttgatg ggcttatgta ccgcaacatt aagagagata agctgaccga ggcggaacaa
    42061 gattatcttg aaaatcatgt cttcattacc tcggctttgt acggtgttgt tccagccttg
    42121 tcacccatgg ctcctcaccg tttggatttt ttgatgaaat taaaagtcgc tggtaagact
    42181 ttgaagagcc attggaaggc agtctatgat gaagctctga agaaggaaga agtgattttc
    42241 tctctcttgt catcagagtt tgagactgta ttttctaagg aaatcagagc aaagatggtg
    42301 accttcaaat tcatggagga tagaggcggt cagctgaaga ttcactcaac tatctccaag
    42361 aaagcgcgcg gggcctttct aacagcttta atagaaaatc aagtacaaac tgtgggggaa
    42421 gcacgtcgct tgaactttgc tggatttgtt taccgagaag atttgtcaca accacaggga
    42481 ttggtttttg taaaggaagt atagaaatca gataagagaa aagtcagcac gattatgctg
    42541 acttttttga taacttataa agtattggac aaatgaatct catttgataa ggtgttaaga
    42601 tgattaacca aattcatgat tgacagatga tttcggcaaa taaacttctt ggtaatccct
    42661 gaaatataga aattagacac gatgatatcg taatctgtct gatttaaaat ctcaggactt
    42721 gtttttaatt catcccaaat atcgaaggta aagcggttat tacagtaata ggaaagcata
    42781 tcaacaaagg taagggatat agcatggtca aagttactga taatcaaaac cttgataggt
    42841 ggtcgatttt ctaacaactg agtagagagg ttttcagcat gggttaagat ggtgtacatc
    42901 aagtgttcca actgttcagg atggtcatgc tgtccaattg cctgacggta ttgagccagt
    42961 tcttgacgcg cacttcccat aaagtctggg aagtaacctt caaatttttt aatcgtcaat
    43021 gctttttgtt caaataagat tggggtagaa aagatttcct gtctttcaaa gaaagcagtg
    43081 ttgtgtaaat gccaaatcag ctcatcgtgg ttagtaaaag taatcttata ttgttttgat
    43141 aggcgttcta atatttgact aagtaattgg taagagtatc ttgcttgact gttatcttct
    43201 aaagagttga agaattcttg aggatctagg aaaatatcat tctgaagaaa agaaataaaa
    43261 atttgagcga ttgtatctgg agtcatttca agcccaaaat gtttagagaa ataagcaagt
    43321 gtctcctgga aatttggaat gctctttaaa agaggataat attctttgta aaagtggttg
    43381 gggaggtcaa tgaagtgacc atttttaacc ctgtggatac tgatagctat catcagtttg
    43441 tacattctat aaattgaaaa tcgcattgga taattcgtga ttttatagaa gaagtcagca
    43501 aactctgtta aatcttcctc aggaaggtca ggaaaaggcc aatctaggaa ataatagcgc
    43561 tcagaaaagt attgggcaaa aaagtaacga atatcgatct catttccacg tatctcggat
    43621 ggagtaaaag ataattcaat ttgaaactga gatgaaagaa cctttgtaat atttctgccc
    43681 agtcgataga ggttggcact actaaagtaa agattttcta ttgttcgata aataggtagc
    43741 ccctcattga agaacatata ctctagtaat tgaaaagatt gagaattagc aagaaaatat
    43801 tgataaatat cttctacact agtgttaact tctaaatcta tcatgattcc gttaacagaa
    43861 gattggatgt taatagatgg aaaggcaata cgtaattcat ttaaatcact ttttaagatt
    43921 cgttctgtac atcctaattt ttcggctagt tcatgtaatt tcatccaatt acgattctga
    43981 attagggttt ccattaagcg taattgtctt tgaacttttg tggataaaag gtttctcata
    44041 cgggtatttc ctccataatt cattatatct ttttttgtct aagtagtata aaaaaattag
    44101 agcaagaaaa ttgtcgttag taatcggaaa aattagaata atataagtgg cttttaagtg
    44161 caaaaacaga atatattaat tgttcagaaa atattgatta atgcctcaaa tagttgtaca
    44221 ttgtaaacta taaaatacta tctaggagtt taaaatgaaa cattcacata aaaaatcatt
    44281 tgactggtat agcatgcaac aacgttattc tattcgtaag tattactttg gtgcagttag
    44341 tgtcttgctc ggtaccgctt tggtattagg tgcagcagct agtgtccaaa cagtacaagc
    44401 ggaagaaaac aaacaagaaa ctaccaatag tatttctgtt ggtaggggag aagcagctac
    44461 taaaccagca gaggtttctg cgtctaataa agagaaaacc tatgcagctc caactgtagc
    44521 taatccagta gaaacgactc cagttaaaac tggagaagtt actaaaccag cagaaaaagt
    44581 tgaagaagca aaagacaaaa aagaggaagt aacgcatcaa gatgctattg acaagtcaaa
    44641 attgttaacg gctctttcgc gtgctaaaaa gttagaaagc aagttatata cagaagcaag
    44701 tgctgctaac ttgcaaacaa gtatccaagc tggtcaaagc ttgcttggga aagcagatgc
    44761 atctgaagct gaattatcag cagcagagtc atctattcaa tcatctatta ttggtctaga
    44821 acttcgttct aactctaaca aaggaactgt atcagaaact cctgtagcga agaaagctaa
    44881 tatagttgaa gcgaaggaag agactaatcc agctgcaaca actgatcgtt cagctgttga
    44941 tagtgctgtt ttgccaacta gcacagctgc caaagtagaa acaacttcag ctccagcatc
    45001 tattaatgaa atcttgaaac caggtttgag cctttctgat gctcgccaaa atccagctat
    45061 ccgtaaggaa gatgttgata gagggtatag tggttttaga gcggcgcctg ctccaacgaa
    45121 ccaaccagta actaatctag gaaataatac tgttttcact gatattagca gaggttctca
    45181 tactttccgt ggtaatggaa attcacgtcg tggaaacccg attcattttg atgtaacaac
    45241 aactagagtt agaaatagag tagatttctc aatcagatat tcaggaccag gtgagtttgt
    45301 taataacaac tttatcttag acaaaggaaa tggattcgga gaaccttctc gtgcaactgt
    45361 ttcaacacca aaattaagag accaagttaa aaacattagt aagggtggaa actttgtttc
    45421 tcattcaggc tacagtttag aagctgcagt agaaacaagc accaatcaaa caattaagtt
    45481 tagtttacct atagccaacc ctaatggtga tctgtctgtt cgattaagac ctgttacttt
    45541 taatgtaggt caaggggaat caggatctgc aactagtaat gatccatata gtaactctaa
    45601 ctattacttc agagaaaaac cactcctctt ggatgcaaat ccaaatggtg gtactaataa
    45661 taagactgtt tcagaagaca ttgacttcca aactgtctat cttccaacta gtaagttacc
    45721 agaaggtcaa actagattag ttcgagaagg tgaaaaagga caacgtcaaa tcatctataa
    45781 agttcatcaa ttgggtaacg aaacaatcct aggattgcca attagtaatt cagttattaa
    45841 agaagttaag ccacgtatta tgcaaattgg tgtggctaaa gatctaatcg atacagtaaa
    45901 accacgtgtt gatcaaaata aagtcggtga tacaaataac ctcactttct atcttgataa
    45961 cgatggaaac ggtgtttata ctgagggcgt agacgaactt gttcaaaaaa ttgctattaa
    46021 agatggtgct aaaggtgaaa aaggagaccg aggcgaacgc ggtctaactg
//
LOCUS       DAVBCN010000008        41136 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVBCN010000008 DAVBCN010000000
VERSION     DAVBCN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41136
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.008"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            102..1127
                     /locus_tag="VQ497_000412"
     CDS             102..1127
                     /locus_tag="VQ497_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HET2516772.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            1117..2124
                     /locus_tag="VQ497_000413"
     CDS             1117..2124
                     /locus_tag="VQ497_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2516773.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLATFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            2124..3131
                     /locus_tag="VQ497_000414"
     CDS             2124..3131
                     /locus_tag="VQ497_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2516774.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            3131..3925
                     /locus_tag="VQ497_000415"
     CDS             3131..3925
                     /locus_tag="VQ497_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2516775.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
     gene            4313..4702
                     /locus_tag="VQ497_000416"
     CDS             4313..4702
                     /locus_tag="VQ497_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2516776.1"
                     /translation="MPKRYLLDGKTPRISATNIDNGILGYYEDIDDKNYRVFENFISV
                     SFLGAVFYHKYKASLDMKIHCLKLKNKELNKEVAFYLTSIIRQALKNTEYKDQISSTV
                     LPDIKIKLPIDSRGTPDWNYMERYRDR"
     gene            4668..4982
                     /locus_tag="VQ497_000417"
     CDS             4668..4982
                     /locus_tag="VQ497_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516777.1"
                     /translation="MGITWKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYKNLK
                     EKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYDVGE
                     SL"
     gene            5666..5827
                     /locus_tag="VQ497_000418"
     CDS             5666..5827
                     /locus_tag="VQ497_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516778.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            5919..7598
                     /locus_tag="VQ497_000419"
     CDS             5919..7598
                     /locus_tag="VQ497_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET2516779.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCNHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKVDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            7866..8432
                     /locus_tag="VQ497_000420"
     CDS             7866..8432
                     /locus_tag="VQ497_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516780.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKTYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            8754..8942
                     /locus_tag="VQ497_000421"
     CDS             8754..8942
                     /locus_tag="VQ497_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HET2516781.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            9004..9762
                     /locus_tag="VQ497_000422"
     CDS             9004..9762
                     /locus_tag="VQ497_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET2516782.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYALS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVVF"
     gene            9820..10674
                     /locus_tag="VQ497_000423"
     CDS             9820..10674
                     /locus_tag="VQ497_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET2516783.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNLTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCNIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            10786..11670
                     /locus_tag="VQ497_000424"
     CDS             10786..11670
                     /locus_tag="VQ497_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HET2516784.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            11833..12606
                     /locus_tag="VQ497_000425"
     CDS             11833..12606
                     /locus_tag="VQ497_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HET2516785.1"
                     /translation="MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMNYLQDLGITGLYLCPIFESTSNHKYNTTDYFEI
                     DRYFGDKETFRELVDQAHHRGMKVMLDAVFNHIGSQSLQ"
     gene            12673..12960
                     /locus_tag="VQ497_000426"
     CDS             12673..12960
                     /locus_tag="VQ497_000426"
                     /inference="COORDINATES: protein motif:HMM:NF012356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylase"
                     /protein_id="HET2516786.1"
                     /translation="MTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYW
                     IEEFNIDAWRLDVANEIDHQFWKDFRKAVLAKNPDLFEIIKNIRNLVINLN"
     gene            12909..13897
                     /locus_tag="VQ497_000427"
                     /pseudo
     CDS             12909..13897
                     /locus_tag="VQ497_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050775.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            14066..14128
                     /locus_tag="VQ497_000428"
     CDS             14066..14128
                     /locus_tag="VQ497_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHP2/SHP3 family peptide pheromone"
                     /protein_id="HET2516787.1"
                     /translation="MKKISKFLPILVLVMDIIII"
     gene            14209..14757
                     /locus_tag="VQ497_000429"
                     /pseudo
     CDS             14209..14757
                     /locus_tag="VQ497_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059560.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14750..16063
                     /locus_tag="VQ497_000430"
     CDS             14750..16063
                     /locus_tag="VQ497_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2516788.1"
                     /translation="MTRKVPNIEQMSQIECGLCCCLSILHFYKSKETLLDLRRDIEKG
                     RDGYSIGDLKQLLNKRNFDTGSYQVKDVNKISELPLPLIAFWDNQHYVVIYKVKKNKV
                     YIMDPSKGYINYEFKEFSKHFSNIVLLSFPNENYQSLKSQFPSPWIRVFSSFSKVKGR
                     LILTLLFSIISYLIILSVPVMTSKFINSALGNTFSFQTSFLILFSLLCLYLISILARS
                     MGILFSNIFFSRDIESFTFKHLLKLPYSFFELRAKGDILYRISSLSGFRELFTNQVVG
                     GVVDIGTILSVVIYMFLSSKTLSIIALILSLINFLFLFSTRKIMYDTVNRELQEQSLI
                     YSVETEALNTISSIKISGLEDEIYENWSKYLKNVLTKYKKRSIVHILYNSATNVFQLF
                     APIIILIFGLDNVLNGKILLGEVVAFQTMASILFSSEISIFNAYT"
     gene            16232..16843
                     /locus_tag="VQ497_000431"
     CDS             16232..16843
                     /locus_tag="VQ497_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2516789.1"
                     /translation="MTIEPGQRIALVGQSGSGKSTLSKILSGLYKIDTGKILFDGVNI
                     NQIDKKILSQNLGVVPQDSFLLNRSILDNITLKNEVTSQKIEEVCKAVQIYDEIMAMP
                     MKFNTIISEMGSNISGGQRQRIALARALINNPSIVILDEATSALDTINEERITKYIQS
                     QDCTQIIIAHRLSTIKDADIIFVMKGGKIVESGNHKYLMDLGG"
     gene            16886..17056
                     /locus_tag="VQ497_000432"
     CDS             16886..17056
                     /locus_tag="VQ497_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516790.1"
                     /translation="MKKVNEYVISTAASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            17090..17818
                     /locus_tag="VQ497_000433"
     CDS             17090..17818
                     /locus_tag="VQ497_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2516791.1"
                     /translation="MAIVEIINLTKSFKDIEVIHNTSFYLNKGKVYGFVGPNGAGKTT
                     IIKMILGILKPDSGKITIFNQTVEQNSKNILSRIGLVLGPSFYGHLDAYKNLKLIANM
                     KGLSLDTERLNEYLSMVGLKDVKKKKVKNFSMGMKQRLSIAASLLGSPEILIWDEPIN
                     GLDPQGVIEIRSLIRFLQEKKGITFLISSHILSELDKVISDIIIINYGKVEFFGSCHY
                     LLQKYNCRNLEEAYLACLAGGEYD"
     gene            17811..18548
                     /locus_tag="VQ497_000434"
     CDS             17811..18548
                     /locus_tag="VQ497_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2516792.1"
                     /translation="MIKLEFLKQKKSILWFVLIFPIILNALLYIDLTFRYRGYLLVHQ
                     NELALSNWQLIFKEQTIFYFSELFYLVLSLIIYEVFAVEFKNDAWLTVISLPFRNKYT
                     INSKLLITVVYTFTFWLSDYISLYVIGKAIDNSLEIGLIFFLKTFTIQLISSLMIMLL
                     YFLTLVLIRKISGIIPIGIIMMILTISIYYNDYNFKIYLPFTYLSHAFRVTESQFYMI
                     LLSNIIIIVLFYILIRKLNERSFEMKL"
     gene            18561..19286
                     /locus_tag="VQ497_000435"
     CDS             18561..19286
                     /locus_tag="VQ497_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516793.1"
                     /translation="MKLLKNELIKSKIFLFIIVDICIQILVILAIKTYILDISAPYSE
                     LDYNKYWYVLHALIYMPMIFPIQILYQNLREALIEDNNNGWNIMVINTNNLVKIIYIK
                     VTINIVRCFICYFVYTIFSLIQLGGMGTDMLLTNIIFPNIIFPNIMSFLLFLPIAIFM
                     QICCIRFDSILAKALPNILLILIVLITFQSDWNIFIPATYYYTEIQSTTNLGIKLLVC
                     IWIMGFEFSLLPKLIKLKEQNLV"
     gene            19829..20050
                     /locus_tag="VQ497_000436"
     CDS             19829..20050
                     /locus_tag="VQ497_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2516794.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
     gene            20567..20806
                     /locus_tag="VQ497_000437"
                     /pseudo
     CDS             20567..20806
                     /locus_tag="VQ497_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            20950..22179
                     /locus_tag="VQ497_000438"
     CDS             20950..22179
                     /locus_tag="VQ497_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET2516795.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            22199..24895
                     /gene="ppc"
                     /locus_tag="VQ497_000439"
     CDS             22199..24895
                     /gene="ppc"
                     /locus_tag="VQ497_000439"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET2516796.1"
                     /translation="MSLQKLENSSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATNLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYEIILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    24978..25195
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            25308..26312
                     /locus_tag="VQ497_000440"
     CDS             25308..26312
                     /locus_tag="VQ497_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2516797.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     IELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTIKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            26381..27247
                     /locus_tag="VQ497_000441"
     CDS             26381..27247
                     /locus_tag="VQ497_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2516798.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            27244..28002
                     /locus_tag="VQ497_000442"
     CDS             27244..28002
                     /locus_tag="VQ497_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2516799.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            28092..29852
                     /gene="dnaG"
                     /locus_tag="VQ497_000443"
     CDS             28092..29852
                     /gene="dnaG"
                     /locus_tag="VQ497_000443"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET2516800.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMERISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            29855..30964
                     /gene="rpoD"
                     /locus_tag="VQ497_000444"
     CDS             29855..30964
                     /gene="rpoD"
                     /locus_tag="VQ497_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET2516801.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            30978..31307
                     /locus_tag="VQ497_000445"
     CDS             30978..31307
                     /locus_tag="VQ497_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET2516802.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            31422..32465
                     /gene="cpoA"
                     /locus_tag="VQ497_000446"
     CDS             31422..32465
                     /gene="cpoA"
                     /locus_tag="VQ497_000446"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HET2516803.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            32481..33806
                     /locus_tag="VQ497_000447"
     CDS             32481..33806
                     /locus_tag="VQ497_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2516804.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGILIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGALYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFRTVLYLPQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            33837..34013
                     /locus_tag="VQ497_000448"
     CDS             33837..34013
                     /locus_tag="VQ497_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET2516805.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            34072..34206
                     /locus_tag="VQ497_000449"
     CDS             34072..34206
                     /locus_tag="VQ497_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HET2516806.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            34270..35580
                     /gene="obgE"
                     /locus_tag="VQ497_000450"
     CDS             34270..35580
                     /gene="obgE"
                     /locus_tag="VQ497_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET2516807.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            35590..35748
                     /locus_tag="VQ497_000451"
     CDS             35590..35748
                     /locus_tag="VQ497_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516808.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            36798..38057
                     /locus_tag="VQ497_000452"
     CDS             36798..38057
                     /locus_tag="VQ497_000452"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET2516809.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            38073..38624
                     /locus_tag="VQ497_000453"
     CDS             38073..38624
                     /locus_tag="VQ497_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2516810.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            38617..39894
                     /locus_tag="VQ497_000454"
     CDS             38617..39894
                     /locus_tag="VQ497_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2516811.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIDRLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            39910..40770
                     /locus_tag="VQ497_000455"
     CDS             39910..40770
                     /locus_tag="VQ497_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HET2516812.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
BASE COUNT        13672 a         6290 c         8429 g        12745 t
ORIGIN      
        1 ccttttttga aaaaattata ttattaaatt tgtaactgta tctattgaca atgataatta
       61 ttatcgatac aatagacttg aaatatgttt aaggagtttt tatgaaaaac aaattttttc
      121 taatagctat tttagctatg tgtatagttt ttagcgcttg ttcttctaat tctgttaaaa
      181 atgaagaaaa tacttctaaa gagcatgcgc ctgataaaat agttttagat catgctttcg
      241 gtcaaactat attagataaa aaacctgaaa gagttgcaac tattgcttgg ggaaatcatg
      301 atgtagcatt agctttagga atagttcctg ttggattttc aaaagcaaat tacggtgtaa
      361 gtgctgataa aggagtttta ccatggacag aagaaaaaat caaagaacta aatggtaaag
      421 ctaacctatt tgacgatttg gatggactta actttgaagc aatatcaaat tctaaaccag
      481 atgttatctt agcaggttat tctggtataa ctaaagaaga ttatgacact ctatcaaaaa
      541 ttgctcctgt agcagcatac aaatctaaac cttggcaaac tttatggaga gatatgatta
      601 aaattgattc aaaagcctta ggtatggaaa aagaaggtga tgagttaatc aaaaatactg
      661 aagctcgtat atccaaagaa ttagaaaaac atccagaaat caaaggaaaa atcaaaggaa
      721 aaaaagtatt atttactatg attaatgctg cagatacatc aaaattctgg atttatacta
      781 gcaaagatcc aagagcaaat tatttaacag atttaggtct agttttccct gaatcattaa
      841 aagaatttga gagtgaagat agttttgcaa aggaaatttc tgcagaagaa gcaaataaga
      901 taaatgatgc tgatgtaatc ataacttatg gtgatgataa aactcttgaa gctttacaaa
      961 aagatcctct tttaggtaaa ataaatgcaa ttaaaaatgg tgccgttgct gtaattccag
     1021 ataatacacc gttagcagcc tcatgcactc caacaccact ttcaataaac tatactattg
     1081 aagaatacct aaatctttta ggaaatgcat gcaaaaatgc gaaataaaaa acaaataaac
     1141 ctaggcataa tttttgtaat ctgcctaggt cttcttatta caatattttt gtcattaaag
     1201 cttggaacaa aagaaattaa tatcagagat tttttagcaa cttttggaat gggcaataca
     1261 aatgatgatt ttattaaatc aattatatat aaaagaatac ctagaactat ttttgcaatt
     1321 ttagcaggtt ctagtcttgc cataagcggt gtattgatgc aatcagttac tagaaaccca
     1381 atagctgatc caggtatact cggtataaac acaggagcaa gtcttagtgt agtaattggt
     1441 ctttcttttt taggaatttc atcaagcata agccatataa gttttgcaat cattggtggc
     1501 ttagtaagtg caatttttgt atacgcgatt gctgtaagcg gaaaagcagg ccttacccct
     1561 ataaaactag ccttatcagg aacttgtgtt agtatggctt taagcagttt tgtaagtttt
     1621 ttaattttac cgaataataa cgtcttagac aaatttagat tttggcaaat aggtagcctt
     1681 ggagcagcta cattatcttc tatatctaca ctactacctt ttataatttt aggtcacttg
     1741 atagctatat ttatttcatc agatttaaac gctttagcta tgggtgatga aatggctgtt
     1801 ggtcttggag ttaatgttaa taggataaga tcacttgcaa taattgcaag tgtgctttta
     1861 tgttcaagta ttactgcaat tggtggacct attggcttcg taggtcttat agttcctcac
     1921 ttttgtggct tatttataag caaagatata cgcacaatga ccatttcttc atcttttata
     1981 ggtgcagagc tcttgcttat atgtgatata atcggccgta tgttaggtaa accaggtgaa
     2041 attgaagtag ggataattac tgcaataatc gggggtccag tacttattta tgtaactatg
     2101 aaaaatagag gggttaataa ctaatgcaaa atttaattat aggtattcaa aaaagaaaaa
     2161 atagaataac actattttcc tcactatttt tattaataat aatcagtcta tcatttttca
     2221 ttttacttat cggagatgaa agttattctt tttcaacttt gattaaagtc ttaaatagtg
     2281 aaactgttcc tggagctagt ttttcgatta tggaaattag attaccaaaa ttattagcag
     2341 gaattatagc tggctggtct tttggattgg caggatttat ctttcaaact atgttaagaa
     2401 atcctcttgc aagtcctgat ataatcggtg tcacaagttc ttcatctatt gcagcggtct
     2461 tttgcatatt ggtattaaaa acaaatagtt taactactgg aattatttca ataacttgtg
     2521 gactaacatc atctttaata ttatttttac tagctaaaaa agatggtttt tcagcagcaa
     2581 gactgataat attaggtatt ggttttcaag ctgtcacaag agcaggcacc tcatttttat
     2641 tgttgaaagt agcaagatat gaattacaag aagttatgag atggctcagt ggctctttat
     2701 cttttacaaa gttagatgac atacctcttg ttctaatagt aagtattatt gctactatat
     2761 tagttttatt ttttaataaa agactagaaa ttattgaact tggtgaagaa atagcaatcg
     2821 gacttggagc aaatcccgag ctttcaaggc ttgttttaat tttttgcgct gtatctttaa
     2881 ctgctttttc tacttcaatt acaggaccaa tagcttgtat atctttttta gctggtccca
     2941 tagccttaaa tattggcaag aaaagaagcc caatattagc tggattggtt ggaattttac
     3001 tagttttgtt atcagacata ttctctcaaa atattttacc agctagatat ccagtaggtg
     3061 ttgtaactgg cttgttaggt tcaccatact taatatactt actaataaaa atgaacagga
     3121 ggaatatata atgaaaggtc tgtggtcaaa taatcttacc tgcggttatg atgaaaaaat
     3181 aatcttggaa aatataaata taaaaatacc tgaagaaaaa atatcagtta ttattgggtc
     3241 aaatggttgt gggaaatcaa cactcattaa aaccttgtct cgacttataa agccattaga
     3301 gggagaagta ttgcttgata ataaatcaat taattcttat aaagaaaaag atttagcaaa
     3361 acacatagct atattacctc aatctccaat aatccctgaa tcaataacag tagctgatct
     3421 tgtaagccgt ggtcgtttcc cctacagaaa gccttttaag agtcttggaa aagatgacct
     3481 tgaaataata aacagatcaa tggttaaggc caatgttgaa gatctagcaa ataacctagt
     3541 tgaagaactt tctgggggtc aaaggcaaag agtatggata gctctagccc tagcccaaga
     3601 tacaagtatc ctacttttag atgagccaac tacttacttg gatatctcat atcaaataga
     3661 actattagac ctcttgactg atctaaacca aaaatataag acaaccattt gcatgatttt
     3721 gcacgatata aatctaacag caagatacgc tgattaccta tttgcaatta aagaaggtaa
     3781 acttgttgca gagggaaagc ctgaagatat actaaatgat aaactagtta aagatatctt
     3841 taatcttgaa gcaaaaatta tacgtgaccc tatttccaat tcgcctctaa tgattcctat
     3901 tggcaagcac catgttagct cttaataatt gaatattgga attcatttaa aagtcgcaaa
     3961 ttttgaactg accctcaaaa gttaggccaa aaaactaact aaaggaggtc agttttttaa
     4021 tggcaaatac agcacagaat ttaagataaa attagtaaaa gaatatcatg aatgaaaaat
     4081 aggatataga gaataacagc aatattaaaa agatcaggac tgataataaa caaaaagaaa
     4141 gtacaacgac tagttcaaaa gctaaaactt caagtaaaaa gttattcaag aaaatctaga
     4201 aaatactcat cctacaaagg acaggtagga aaaatatcag acaacaaaat aaaaagaaat
     4261 atttattacc gaaaaaaagg ggaaatcgag tcaagtacca acaggctcat ctattccaaa
     4321 aagatattta ctcgatggaa aaactcctag aattagtgct acaaatattg acaatggtat
     4381 cttaggttac tacgaagata tagatgataa aaattataga gtttttgaaa attttatatc
     4441 tgtttcattt ttaggagcag tcttttatca taaatataaa gcaagcctag atatgaaaat
     4501 tcattgttta aaattaaaaa ataaagaatt gaataaagaa gtagcattct atttaacttc
     4561 aattattagg caagctttaa aaaatactga atataaagat caaatatcta gcacagtatt
     4621 gccagatatc aaaattaaat taccgataga cagtagaggt actccagatt ggaattacat
     4681 ggaaagatat agagatagat aaagagagag agggagagat agataaaaag tccaccccca
     4741 ttttttatgg agaattcaaa aatgtaaggt taagcaaaga agaatataaa aatctaaaag
     4801 aaaaattaaa ctcacacaca gatataatga taaataaact atccagatac atggaaagca
     4861 gtggtaagac ctatcaaaac cactatgtga caatcttaaa atggtatgaa gaagataaag
     4921 acaaactaag acagaaaggt ttaaataaaa aaatgaatta tgatgtagga gaatctttat
     4981 agataaaaag aggtggaaag gcactattaa agactttaag tctaaaaagg tatcaaagta
     5041 tgccagagat aaaaaaatat aaatatgaca ctttaaaggc gattagaaaa ttaaagaaat
     5101 gctagaagac ttttagatga cagttaccaa gagggtctaa ttttcactgc tacttatgca
     5161 gtgggaattt gaccttttta tataagcaaa ttctatttca aaattggtta ccaagaaaga
     5221 cttcttttca ctgttaagta taagagggta atttatagaa ttttgttaca ttaagataaa
     5281 tattttctga tggtcaaatt ttaaaaagat taataactct cgataaattt tgaaccttaa
     5341 caattgaata gaccaagaca agacgttaat tatttttgga gcgtaataac ctatccgtca
     5401 agatctttcg gctgaataat tcggcaaaaa aagttctgct aagtaaaaag aaagtgagcg
     5461 ataagtaaga gtttgaatat agggagggat accctatccg ctatgaagaa aatgaggata
     5521 atgatacttc taaggttgaa gccggctcat ccggaataga ggcggaggtg agattcctat
     5581 gttgctaatc caaagcactt gtcaatgtca aaaaacttaa ttaaacaaat aattattaga
     5641 tataaaactt tagaggaggt atatattgga caatgactgg aatggattgg ctgatttaat
     5701 agcaaatctg attgcaaaat atgctggtgc tttagattta gataatctac ccgatccaac
     5761 gccagcaaaa aatcaagaga tgaaaaatag ttttgacatg gcaaaaacac aaattgagac
     5821 ggactaataa aagtgttata atatacttga aatgatagtc caaacttaaa ttaaaaaaat
     5881 tatatatgat atagaaaaat tatattgaga ttaatactat gtcaaaagaa aaaataaaag
     5941 tatacctcta tacacgagta tctacgtcaa tacagataga ggggtattcg ttagaggcac
     6001 aaaaatcacg aatgaaagct tttgctattt acaacgatta tgaaattgtt ggagagtatg
     6061 aagatgccgg gaagtctggc aagtctattg aaggtagaat acagttcaat cgaatgatgg
     6121 aagatataaa atctggaaaa gatggagtat cttttgttct tgtgtttaag ttatcaagat
     6181 ttgcaagaaa tgccgctgat gttctttcta cacttcagat aatgcaggat tatggagtta
     6241 atcttatctg cgttgaagat ggcattgatt catctaaaga tgctgggaaa ctaatgattt
     6301 ctgttttatc agctgtggct gaaatagaaa gagaaaacat ccgtattcaa acaatggaag
     6361 gtcgcattca aaaagcaagg gaaggaaaat ggaatggtgg ctttgctccg tatggatata
     6421 aacttgaaga cggcaagctg tttataaatg aggaagaggc agttgcaata agaacgattt
     6481 tcgaccagta tgtaaatact acgataggag ccaatgggat atctaaatac ttagagaatc
     6541 atggaattag aaaaatccca agacagaatg gtaagaatcc tttgtttgat gcaggtctta
     6601 taagaaagat attaaagaat cctgtatata atgggaaaat agcatttgga agaagaactt
     6661 tagaaaaagt tcatggtaca agaaatgaat ataagcaggt tgaacaagat gaatatctaa
     6721 tatctgaagg gatacatgaa gctatagttt ccgatgaagt ttggcaagct gctcaggtta
     6781 agctaaaatc tcaagcaaag aaatatgagc atgtgaataa aggaaaagat acacgcacac
     6841 acttgctttc tggaattgta aaatgcccga tatgtggagt gggaatgttt gggaacaagt
     6901 gtatcaagaa aaagaaagat ggcacaaagt ataaagattt ttattactat ggttgtaacc
     6961 ataggcagat gataagaggt cataagtgta cattcagtaa gcaaattaga gaagaattgt
     7021 tagatgatgc tgttgcagag gtgattgtca agatagtaag taatccgaaa tttgcttcta
     7081 tgatgcaaga aaaaatcaac atgaaggtgg atacctctga aatagaaaaa gaaatagata
     7141 attaccaaaa ggaattgaga aagagtcatt ctacaaagtt taagctaatt gaggaaatag
     7201 ataatttaga tgttgaagat aagcattata agagaaggaa acaggattta gatgatagac
     7261 tatatcgtat gtatgacaaa gtagatgaat tagaatcatc actaattgat gcgaaagcaa
     7321 agaaacagac tattgaagct gaaaagctta caggagataa catatataag gttctgatct
     7381 attttgataa gctctataaa gtcatgaatg atgtagagcg taggcagtta atttcagctt
     7441 tgatttctga aattcaagtt tatgaagaaa aacaatctaa cggacaatgg ctaaaatcca
     7501 ttacttttaa gctaccaatt atagaagaaa atctcaacat aggtttggac aatgatgaac
     7561 aagttgaatg tgtaagttta ttagagaaac gtagttaatc ctcaaaaggt tccccgaacc
     7621 ttttgagtcc cactgacgca tcacgctgag gcggtatcac tgcttgtacg agttgaagca
     7681 tcagcgaagt aaaagtagat gttcagccaa tgcaacgagg tgagaccgaa ggtcgataag
     7741 gtgtgttctg ggtataggac tcgtaaagta agtaggaaat atgatgagat gatacagaga
     7801 gcctgaattg gtcaggtgac gtgcttattt acattagcct gataatgaaa atggagagat
     7861 tatcgatggt tatattggaa ttgtaccaaa atgattattc taaagattta gtagcatttg
     7921 attccataga agatgggaaa gcctttgtag ctcaaatccc gggatacact ctggaaacag
     7981 aagatggttt tgaagtggag tattttaatc ctaaaaacat accggattat atggaaatta
     8041 tttttaacgg aaatattgtt cctttatcta aatttatgtt cgatcctgag gaaaatgtaa
     8101 acattatttg gaaagagatt tcaaatttat ccctcaaaaa tgatagagtg atcgaaggat
     8161 attcaaaaat tgatgcatat gtcgttaaca accatgaagt taaaacctat gtcgaaacaa
     8221 gagaaacgaa ctatcgtaaa gcaaaagact tcttagaaag ccgcggatat gaaatcgaca
     8281 gaagcttttt cggaagtgaa gacggtgaag cgattcttta cagaaaaaaa gggatcgaag
     8341 tttggcattt cttgtgtcac ttagacccaa tgtttgtaga gattgaagat gtagaaggat
     8401 atgttaaaga agaagttgga gagattcaat agtcaaccaa agttccttgt gagtatatac
     8461 ctttgctagt aaaagagtca aaacgttata acgaaggggg ggaagaaaat ccttgagtgt
     8521 aagtcttttt cttgtttaga gggaaagtat tagagtagcc gctcataatt tatactagat
     8581 ttgataactg atttgagaca gattttattt ctataaatat tgtgaaaatt ttttaatacc
     8641 aaagatttaa agttactgtg ttaaaaatcc tctccacttt taaactaatg tattataaaa
     8701 gccagttgac acaagtgtta cacctgtgat agaattaact tgaggtgatg aaaatggcaa
     8761 ccgataaaaa tagaataatg attagcttgg atgataaaaa tctagagaaa cttgaaaatt
     8821 tagtagaaga tgctagagat agaaggggaa tgcgactaac aaagtcccag gttattgaat
     8881 tactcctaaa taccgttgat tatttcgatg atattatggg agctatttac tcaaaaaaat
     8941 aagagcaaaa ttgctattat tttttacaaa tcagtgtaac actagtgata taggagtgat
     9001 gaaatggttg tttatattag acaaagtaaa ttaccaagtg aggtttcaat taataaatac
     9061 aatgctcaag taggtgccta cttacaagga gaagaagtta ttttatatca atctttttct
     9121 gaaataaaag agttaacaag tgaagatata gttgtagatt atataatgga gactagagca
     9181 ttactaaaaa tgatgggctt aaacgttccg gtttatgact atcctattga gcttaaagag
     9241 ttttatggtc gaaagattta tgctggtatt ttaggagaga ttgtgaatat acctgataat
     9301 tggggaaaat ttattaagcc taaagctggt tcaaaagtct ttactggaag agttgttaat
     9361 ggaacccatg atttaatagg tattggtcta cctttcgact atcctatatg gattagtgag
     9421 gttgtagaat tcatagctga atggcgttgt tttgtgttag atggtcgcgt attagatgtt
     9481 cgaccctata caggtgatta tcatgcacaa tttgatgcaa gtgtaattga tgaagcgata
     9541 tcatgttgga aagatgcgcc aatagcttat ggactagata tcggtgttac tcgcgatggc
     9601 agaacacttg ttgttgaagt aaatgatggt tatgcattgg gaaattatgc cctatctcct
     9661 ttaaaatcaa tcaattttca tagagctagg tggaaagaaa tggtaaaacc ctattttgaa
     9721 aaaaatgaaa tttttaaaat ccaacaggat gttgttttct aaaataaata tggtatataa
     9781 ccaatataaa aagcatcaaa atgaacaagg aggaatctga tgcttggagc aattattgga
     9841 gatattgtcg gttctgttta cgaatggaac aatatcaaaa caaaggactt tcctttattt
     9901 cggaaggact gcttttttac agatgacacg gttatgacct gtgctgttgc agaagcaatt
     9961 atgaatggtg gacaaaaaga cgatttcatt gacgcgatga agaaatatgg cagaatgtat
    10021 ccgaatgctg attacggtgc tcggtttaat gcatggctaa acagcgataa ccgtgagcct
    10081 tataatagct ttgggaatgg atcagctatg cgtatttctc catgtgcttg gatcatggac
    10141 tgtggttttt atgcgaaaac tggtatatgg ccatcatcta gaggacttac gagtctttct
    10201 gcagaggtaa ctcataatca tccagaaggt gtcaaaggcg ctatggctac agctgatgct
    10261 atctttctat gtcgttttta ctttggtggt tattgtagag agtacgagca atcaattaac
    10321 gacaatctta cagagtgtaa aagacgaatt aaggattata tagaaaaaga atacgattac
    10381 aatctatctc aaactttaga tgaaatccgt cctaactatc gttttaacga aacatgtcag
    10441 gaaactgtcc ctcaagccat tatcgccttt cttgaaagta gagacttcga agatgcgata
    10501 agaaatgcca tctcacttgg tggcgacagt gatactctcg cagcaatcac ttgtaacata
    10561 gcagaggcag cttacggtat tcctgattgg atcaaggata aggcctattc ttacttggat
    10621 gaacccttaa aggatgtagt taggcgatgg gaaaatagaa taaaagcgta ttaatctcca
    10681 gtacgtcaga tttctctttt ctgtattttc attgtataat aggattacat ttacttgcat
    10741 acgacgaaat gtaaacattt ctgtttaaaa ttaaggataa aatcaatgaa aaaagcaatg
    10801 gtaattatca accctacttc tggtggcgag aaggctttgg attacaaaga aaagctggag
    10861 aataaagcaa aagaatactt tgaatatgtt gaaaccaaaa ttaccgaaaa agcgctggat
    10921 gcaacacatt ttgctgaaga agcttctcgt gagcagtatg atgcagtggt tgtgtttggt
    10981 ggagatggaa ctgtcaatga agtcatttca ggtattgatg agagagacta cattcctaag
    11041 ttagggatta tcccaggcgg tacgggtaac ctcattacaa aacttttgga aatcaatcaa
    11101 gacatcgatg gcgcaattga cgaactggat tttgatttaa ccaataagat tgatatcggt
    11161 aaagcaaatg acaactattt tggttatatc tttagtatcg gttctctgcc tgaggcgatt
    11221 cacaatgttg aaatagagga caaaacaaaa ttcggtattt taacctatgc tgtaaatacc
    11281 atgaagtctg tcatgacaga tcaggtcttt aacattaagg ttgagacaga aaatggaaat
    11341 tatgttggtg aagctagcca tgttttggtc cttttgacaa attacttcgc tgataagaaa
    11401 atctttgaag aaaacaagga cggctatgcc aacattttga ttctgaaaga tgcctctata
    11461 ttctccaaat tatccgtcat tcctgattta ttaaaagggg atgttgtcgc aaatgataat
    11521 atcgagtata tcaaagcgcg taatattaaa atctcttcag atagtgaatt ggagtcagat
    11581 gttgacggag ataaatcaga taacctacct gtagaaatca aagtcctagc tcagcgagta
    11641 gaagtatttt caaaaccgaa agaggattag tatatagaga aagccttttt taaggctttt
    11701 tgtatacttt aaaagatagt tcctttaaca acggacattc cttgcaaata gtttacaaaa
    11761 atagtatact ggatttattg agtttgaaaa cgtttgcgta aaatttgaat gaatacttta
    11821 ggagacaaat tgatggaatt gagtgctatt taccataggc ctgagtcgga gtatgcctat
    11881 ctttataagg ataagaaact ccatattcga attcgaacta agaaagggga cattgaaagc
    11941 atcaacttgc actatgggga cccttttatc tttatggaag agttttatca ggatacaaaa
    12001 gaaatggtca agataacttc tggtacctta tttgaccatt ggcaggttga agtgtcagtt
    12061 gactttgcac gtatccagta tctctttgag ctcagagata cagaaggtca aaatattttg
    12121 tatggcgata aagggtgtgt ggaaaattct ctagaaaatc ttcatgcaat tgggaatgga
    12181 tttaagttgc cttatcttca tgagattgat gcctgcaagg ttcctgactg ggtttcaaat
    12241 acggtatggt atcagatatt tcctgaaaga tttgccaatg gcaatgctct attaaaccca
    12301 gaagggactt tagactggga ttcatctgtc acacctaaga gcgatgattt ctttggtggt
    12361 gatttacagg ggattattga tcatatgaat tacttgcaag acttgggtat tactggacta
    12421 tatctttgtc ccatctttga atctacaagt aatcacaagt acaatacgac agattacttt
    12481 gaaattgacc gttattttgg agacaaggag acctttcggg aactggtgga tcaagcgcat
    12541 catcgtggca tgaaagtcat gctggatgcg gtatttaatc atattggttc gcaatctctt
    12601 caatgaaaaa atgtcgtcaa aaatggtgaa cagtctgctt ataaggattg gttccatatt
    12661 caacaattcc cattgacaac tgaaaagcta gttaataaga gagacttacc ctatcatgtt
    12721 tttggtttcg aggactatat gcctaagcta aatacagcca atccagaggt caagaattat
    12781 cttttaaagg ttgcgactta ttggattgaa gagtttaata tcgatgcttg gcgtttggat
    12841 gtggctaatg agattgacca tcagttctgg aaggattttc gtaaggcagt tttagctaaa
    12901 aatcctgatc ttttcgagat tataaaaaat ataaggaatc tagtcataaa tttgaattga
    12961 aacaacgtct ttattttttg cttcagcaat ctaaatcgtt tgatgatttt ttagagaaag
    13021 caaagcagtt gcatgttcag attgatttta gtcaaaagca tagccgtttt ttgatgacag
    13081 atagaacgat gataaaacca attcgagggc gccaactcag caaacgagat ctatatgatg
    13141 aagaattttt tagaacgcat tttgctaagc aagagattga aagtcgttta gaatttttgt
    13201 taaaccgtgt caattcttta gaagatttaa taacaaaagc aaaagaattg aatctaacca
    13261 ttgacttaaa acaaaaaatg taacttttat cctggaagaa aataatcaaa agataagttt
    13321 gggtcatcaa aaaataagtg ataagaaatt atatgatgtc aaattttttc aagattattt
    13381 taaaaataag gaagtcgttg cttcagaagg attagagaat ttacaggaac agtaccatgc
    13441 tttccaagaa gaacgagata aggataaagt atccactgaa gagattgagg aagcctttaa
    13501 gacatttaag gaaaaatgag ataccgttcg tgaatttgaa gtggaacttg cagagaacca
    13561 aatagagaag ctagttgata agggcgtcta tatcaaggtg tctttcggtg tcaaacagag
    13621 cggtcttatt ttcattccca actatcaatt ggatattatg gaagaagaaa atcataaaaa
    13681 atataaagtc tacattcgag aaacgtcttc ttactttgtc tataataaag aaaacatgga
    13741 taataattgt ttcatcaagg gtaggacctt aatcagacaa ttgagcaacg atagccaaaa
    13801 actcccctac aaagtaatat gtaatttgtc taaaatattg attagtcgaa tacaagatat
    13861 ctctgactcc ccacgttcaa aacgagatat ttgtgatttt gaaagatgtt catctgctac
    13921 agagcataga ctaatttgtt ttcctttacg aacttttcta agtgtgatgc caagttttga
    13981 tttcataatt tcccattttc ccaacaaaaa ttaaatccgt ttttatttat tgtacaataa
    14041 ataaaaacgg aaaggaagtc caagtatgaa gaaaatttca aaatttttac cgattttggt
    14101 tttggtgatg gatattatca tcatttaaaa aagtgtatta attattgtat ttcacaatta
    14161 gataatataa cagtaacagg tttagcacac gattattctg gagtagcaat gtaattatca
    14221 tattattatg aaattaatcc tagtacagat atattaaaat gtatagagga attactatat
    14281 aaagaagata aatgtttcaa taatatatta aaaaattgga aagacatatg aagaaatcat
    14341 aatgattcct ttcctaattt ttggtgttat ggagctccgg gcattttatt agctagaaaa
    14401 gaaattttcg ataaaactaa tattgggaat aatgatttat caataattga aaatgtatta
    14461 actaatgtag aaaaaataag agaattgaat ttatgtcacg gttctgtagg tactatcagt
    14521 tgtcttgatg caatattgaa agatgaagaa aacctattaa taaaagaatc tatagatttt
    14581 tattttgata atgtcgtttc tcaggttatc aaaccagaat taagtacgga tttgaatact
    14641 atgaatacat tttcttttat gctaggagta tctggagttg tctatgaaat atctcgaaaa
    14701 caagatgata gattacttaa tgttttatta ctggagttaa gaggacatga tgactagaaa
    14761 agtacctaat attgagcaaa tgtctcaaat agaatgtggt ttatgttgct gcttgtccat
    14821 actacatttc tataaaagca aagagacatt actagattta agacgtgata tagaaaaagg
    14881 gagagatggt tatagtatag gtgacttgaa acagttattg aataaacgaa attttgatac
    14941 tggctcatat caagttaaag atgtaaataa aattagtgaa cttccgttac cattaatagc
    15001 attctgggat aatcaacatt atgtagttat ttacaaagta aaaaagaata aggtctatat
    15061 aatggatcct tctaaaggat atataaatta tgagtttaag gagttttcaa aacattttag
    15121 taatatagta ctgctatcat ttcctaacga aaattatcaa tctttgaagt cgcagtttcc
    15181 ttctccatgg ataagagtat tctctagttt ttcaaaggta aaaggtcgat tgattttgac
    15241 gttactattt tcaataataa gttacttaat aatactttcc gtaccagtaa tgacttcaaa
    15301 gtttattaat tctgcattag ggaatacatt ttcttttcaa acctcgtttt taattttatt
    15361 ttctctactt tgtttgtacc ttatttctat cttggctagg agtatgggaa ttctattttc
    15421 taatatattt tttagtagag atattgaaag ttttacattt aagcatttgc ttaaactacc
    15481 atatagtttt tttgaactca gggcaaaagg agatatttta tacagaattt ctagtcttag
    15541 tggttttagg gagctattta ctaaccaagt tgttggggga gtagtagata tcgggactat
    15601 tctaagtgtt gtaatatata tgtttcttag ttcaaaaaca ttatcaatta tagctcttat
    15661 attgagttta atcaattttt tgtttctgtt tagtactaga aaaataatgt atgatacggt
    15721 taatagagaa ttacaagaac aatcattgat ttactcagta gaaacagaag cattaaatac
    15781 tatttcttca ataaaaatat cgggattaga agatgaaata tatgaaaact ggtctaagta
    15841 tttaaaaaac gtattgacaa agtataaaaa acgaagtatt gtgcatattt tatacaattc
    15901 agctactaat gtatttcaat tgttcgctcc tataattata ttgatttttg gacttgataa
    15961 tgttttaaat ggaaaaattt tattgggtga ggttgtcgca tttcaaacga tggcatcaat
    16021 tttatttagt tcagaaatct caatatttaa tgcttatacg tagtatattt tggcagcagg
    16081 atacttaaat agagtaaatg atatctggct tgaaaatgaa gagaatgttg aaaatggtct
    16141 taaaaagtgt tctttagaag gtagaataga tattaaggac ctatctttct cttattccaa
    16201 agactcagct cctgtaatag aaaatttaaa tttgactatt gagccaggac agagaattgc
    16261 actagtcggt caatcggggt caggaaaaag tacgttatca aaaatactat caggattata
    16321 taagattgat acaggaaaaa ttttatttga tggtgtaaat attaatcaaa tagataaaaa
    16381 aatattaagt caaaatttag gagtagttcc acaggattca tttttattga accgaagtat
    16441 tcttgataat ataactttaa agaacgaagt tacttcacaa aagatagagg aagtttgtaa
    16501 agcagttcaa atctatgatg aaatcatggc tatgccgatg aaatttaata ctatcatctc
    16561 agagatgggg tcaaatattt caggtgggca aaggcaacgg atagcactgg cacgtgcatt
    16621 aataaataat cctagtattg taattttaga tgaagcaact agtgcattag acactattaa
    16681 tgaggaaaga ataacaaagt atatacaaag tcaggattgt actcaaataa ttatagctca
    16741 tagattgtca acgattaagg atgcggatat tatttttgta atgaaaggtg gtaagattgt
    16801 tgagtcagga aatcataagt acttaatgga tcttggtgga tagtactaca gcttatatac
    16861 aaaaaggaaa tgaggtgtaa agaaaatgaa gaaagtaaat gaatatgtaa tttcaacagc
    16921 agcctcacta ggggtgatga ttggaatagt atttgcaatt tttttagatt ttccagttga
    16981 atatggtatt tctttaggtt tgttgaatgg aatagtattg ggttcgctga ttgtttacaa
    17041 aaacaataag aattaagcat aattttttgc tgtaaactaa ggagtagaga tggctatagt
    17101 tgaaattata aatctaacaa aaagctttaa agatattgaa gttattcata acacttcatt
    17161 ttatttaaat aagggaaaag tatatggttt tgttggacca aatggggctg gaaaaactac
    17221 aattatcaaa atgattctag ggattttaaa accagattct gggaaaataa ctatttttaa
    17281 tcaaaccgta gaacagaatt ccaaaaatat attatctagg ataggattag ttttgggacc
    17341 ttcattttat ggtcatcttg atgcttataa aaatttgaag ttaatagcaa atatgaaagg
    17401 gcttagttta gatactgaaa gacttaatga atatctatca atggtaggat taaaagatgt
    17461 taaaaagaaa aaagtgaaaa atttttcaat gggcatgaaa caacgtctaa gtatagctgc
    17521 atcattgtta ggtagtccag aaatattaat ttgggatgaa ccaatcaatg gacttgatcc
    17581 acagggtgta attgaaatta gaagtttaat ccgttttcta caggagaaaa aaggtattac
    17641 atttttaata tcaagtcata ttcttagtga actggataaa gtgatatctg atattataat
    17701 tataaattat gggaaagttg aattttttgg ttcttgccac taccttttac aaaagtataa
    17761 ttgtcgaaat ctggaagaag cctacttagc ttgtttagct ggaggtgaat atgattaaat
    17821 tagagtttct aaagcagaaa aaaagcatct tatggtttgt tcttattttt ccaataattc
    17881 tgaatgcttt gttatatata gacttaactt ttaggtatcg aggttattta ttggtgcatc
    17941 aaaatgaact tgctttatct aattggcagt taatttttaa agaacaaaca atattttatt
    18001 tttctgaact attttattta gttctatctt taataattta cgaagtgttt gcagtagaat
    18061 ttaaaaatga tgcatggtta accgtaattt cattgccatt tagaaacaag tacacaataa
    18121 attctaaatt actcattaca gtggtttaca catttacttt ttggttgtct gattatatta
    18181 gcttatacgt tattgggaag gctattgata acagtttaga gattggtttg attttctttt
    18241 taaaaacatt taccattcaa ttaatttcct cgttgatgat aatgttatta tattttctaa
    18301 ctttagtact tataaggaaa atttctggaa taataccaat tggtattata atgatgattt
    18361 taacaattag catatactac aacgattata attttaagat ttacttacca tttacttatc
    18421 tttctcatgc ttttagagtg actgaaagtc aattttatat gattttacta tctaatataa
    18481 taatcatcgt acttttttat atattaattc gtaaattgaa cgaaagaagt tttgagatga
    18541 aactttaata ggtaataaaa atgaagttac ttaaaaatga attgattaag agtaaaatat
    18601 ttttatttat tattgttgac atctgtattc agattctagt cattctagct ataaaaacat
    18661 atattttaga tatttcggca ccatactcag agcttgatta taataagtat tggtatgttt
    18721 tacacgcttt aatatatatg ccaatgattt ttcctataca aattttgtat caaaatctaa
    18781 gagaggcatt gattgaagat aataataatg gttggaatat catggtgatt aacactaata
    18841 atctcgtgaa aatcatctat ataaaagtaa cgataaatat cgtgagatgt tttatttgtt
    18901 attttgttta tacaattttt tctttaattc aattaggtgg tatgggaact gatatgttat
    18961 taaccaatat aatatttccc aatataatat ttcccaatat aatgtcattt ctgttatttt
    19021 tacctatagc tatctttatg caaatttgct gtataagatt tgattccatt ttagcaaagg
    19081 ccttacctaa tattttgctg atacttattg ttttaatcac gtttcagagt gattggaata
    19141 tatttatacc tgcaacttat tattatacag aaattcagtc aactacaaac ttaggtatta
    19201 aattattagt ttgtatctgg attatgggat ttgaattttc ccttcttcca aaactaataa
    19261 aattgaaaga acaaaatcta gtttaaatgt tgctcgaaaa ttaaataatt gaatacgatt
    19321 aacaatgaaa caatacaacg tgagtcatga cttcaaagta attgatttta tgattctggt
    19381 ttcttacgct taattaagca agttgtattc actttatcaa attgttatta ctctaaaaag
    19441 cgtctaacag ctatagagtg tctgtttgct tctctttact tttattttaa ataattcatt
    19501 aaccttccgt aagtgcccaa taataaggtg tgttgaagaa gtctgagata aaatagttct
    19561 cagtcacaaa aaaagctata gattcccatt ggtgaacagc tccctgtcaa gtagacagta
    19621 aaataatata aaatcttaag cgacctgatt cctgaattcc agtggagtca ggttgttttt
    19681 taaccgtctt tcttaatatt gtctcaccta taatagatat gcttatgaga tatgtttaat
    19741 aaattaattt tagttcaaaa tgtccagtta ggacactttg aatgatttct tagttttata
    19801 tattatactt ttctcaggag aaagctagat gtacagacgt ttgagagatt tgagggaaga
    19861 tcacgatttg acccaaaaac aaatagctaa aatactttcg tttacaaact cagcttatgc
    19921 taaaattgaa cggggtgagc atacgttaac agcagatgta ttggtaactc tctcaaactt
    19981 ttacgatgtc agtacagact acctattgga attgacagat tttcctgata aaattcgctt
    20041 tagaaaataa tctcctcaat ttcatagagt gtgaaaatga ggagattttt tatttgttct
    20101 ttgaaaatta aatagtccaa aatggtactt tcctcatttg tggagcaaat ttgaatggct
    20161 cgccatgata agagcgattt taaagccatc aataaaatag agcgatactt tatatgccat
    20221 gatacaaatg atatacaatg atacttctga ccgttcaggc tgccatcgta aaagagcagc
    20281 aagtgaaatt cttatgatga cttcatcagt catgccacgt tgaatgtgtg agtttgttag
    20341 agaaacgcaa ttaatcctca aaaggttccc cgaacctttt gagttccaca gacgcatcac
    20401 gtggagtgtg taagcttgtt gctaaaagcg taaaaacctt ggaacgaaag gaataataga
    20461 cttcctgcga aacaaaaata caatacaata aaactatgga tgatgaagca agtaaacaat
    20521 taagcgatag ccgtttcaag agccttgtag gtgttcagcg cacgagatgt tagctgtgtt
    20581 aaaaacagct tatcaacgca acgtaaacgc gcaaaaggtg gacgaaaaac caaattaagc
    20641 ctagacgatc tcctcatggt aactattcaa tacatgcgag aatagcgcac ttatgaacaa
    20701 attgcggctg attttggcat tcacgaaagc aacataatta gtcggaggtc aatggattga
    20761 agcaactctt attcaaaatg gttttacgat ttcaaattct gccttaattc tgtaaaaaca
    20821 gtaaaattcg aaggattgta aggtaagagt ttttttcttt ctgaaaaaat ggtataatag
    20881 caatcaaaac tagaaaataa aacggaattt ggaacagatt tgtctgtatc ctagtagagt
    20941 ggtgatacta tgaagattag taagaggcac ttattaaatt attccatctt gattccctac
    21001 ttgcttttat ctattttggg cttgattgtg gtctattcga ccaccagtgc tattttaatt
    21061 gaagaaggca agagcgcctt gcagttggtt cgaaaccaag gaatcttttg gattgttagt
    21121 ttgatactga ttgccttaat ttataaattg agactagatt ttttgagaaa tgagcgacta
    21181 atcattttag ttatattaat agaaatgctt ttattgttct tggctcgttt tattggtatt
    21241 tcagtaaacg gggcatacgg ttggatttcg gttgcaggag taactattca gccagctgag
    21301 tacttaaaaa tcattattat ttggtattta gctcaccgat tctccaaaca gcaagaagaa
    21361 atagctactt atgattttca agttttgact caaaatcaat ggcttccccg tgcttttaat
    21421 gattggcgat tcgttctcct agttctgatt ggaagtttgg gaattttccc tgatttagga
    21481 aatgcgacta ttttagtctt ggtttccttg attatgtata cagttagtgg aatcgcttat
    21541 cgctggtttt caaccattct ggcgctcgta tctgccgctt ctgtctttgt cttgaccact
    21601 atcagcctaa tcggtgttga gaccttttca aaaattccag tatttggcta tgtagccaaa
    21661 cgctttagtg ccttttttaa tccttttgcc gatcgtgctg atgcaggtca ccagttagct
    21721 aattcttatt ttgccatggt caatggtggt tggtttggtc taggtcttgg aaactcgatt
    21781 gaaaaacgag gttatttgcc agaagctcat acagactttg tcttttctat cgtgattgaa
    21841 gaatttggct ttgttggtgc cagtcttatt ttagctctct tgtttttcat gattttgcgg
    21901 attatcttgg tcggtattcg agcggagaat cctttcaatg ccatggttgc actcggtgtc
    21961 ggagggatga tgttggttca ggtatttgtc aatatcggag ggatttcggg cttgattcca
    22021 tctacaggag taaccttccc cttcttatcc cagggtggaa atagtcttct agtcttatca
    22081 gtggcagtag cctttgtctt aaatattgat gccagtgaaa aacgcgctaa gttgtaccga
    22141 gaattggaaa atcaaccaat gaaccttctg ttgaagtagg ataaagaaag gatagtttat
    22201 gtctcttcaa aaattagaaa attctagtaa taaaagtgtt gtgcaagaag aagtcttgat
    22261 tctaacagaa ttactggaag atattactaa aaatatgctt gccccagaga cctttgaaaa
    22321 aataatacag ttgaaagaat tatcaacgca ggaagattat caaggtctaa accgtctagt
    22381 gactagctta tcaaatgatg aaatggtcta tatttcacgc tatttctcta tcttgcctct
    22441 tttgattaat atttcagagg atgtggattt agcttatgaa atcaatcatc aaaataatat
    22501 tgatcaggac tatttaggta aattatctac aacgattaaa ttggtagcag aaaaggaaaa
    22561 tgccgttgag atcctagaac acttgaatgt tgttccggtt ttgacagccc atccaacaca
    22621 agtgcaacgc aaaagtatgt tggatttaac aaatcatatt catagtcttt tgcgtaaata
    22681 ccgtgatgtt aagttggggt tgatcaataa agataaatgg tacaatgatt tgcgtcgtta
    22741 catcgaaatt atcatgcaga cagacatgat tcgtgagaaa aaattaaaag tgactaacga
    22801 aatcacgaat gctatggaat attataacag ctcctttttg aaagctgtac ctcatttgac
    22861 gacggagtat aagcgcttag cgcaagcgca tggtctgaat ttaaaacagg ctaaaccaat
    22921 caccatgggt atgtggatag gtggtgaccg tgatggaaat ccatttgtta cagcaaagac
    22981 cttgaagcag tctgcactca ctcaatgtga agtcatcatg aactactatg ataaaaagat
    23041 ttaccaactt tatcgtgaat tttctctttc aactagcatt gtcaacgtca gcaagcaagt
    23101 cagagaaatg gctcgtcaat ccaaggataa ctcgatttac cgcgaaaaag agctttaccg
    23161 tcgtgccttg tttgatattc aatcaaaaat tcaggcaact aaaacctatc tgattgagga
    23221 tgaagaagtt gggactcgtt atgaaaccgc caatgatttc tacaaggatt tgattgccat
    23281 tcgagattct ctactagaga ataagggcga gtccttgatt tcaggtgatt ttgtggaatt
    23341 attgcaggca gtagagatat ttggttttta cctagcatca attgatatgc gacaagactc
    23401 tagcgtctat gaagcctgtg tggcagaact cttgaaatca gcaggaattc attctcgtta
    23461 tagcgagttg agcgaagaag aaaagtgtga ccttcttttg aaagaattag aagaagatcc
    23521 ccgaattctt tctgcgactc acgcagaaaa atcagaatta ttagcaaaag aactagctat
    23581 ttttaagacg gctcgtgttt tgaaagataa gttgggagat gatgtcatcc gtcagaccat
    23641 catttcacat gcaaccaacc tttctgatat gctagaatta gctattctgt taaaagaagt
    23701 aggactggtg gatacggaaa gggcgcgtgt tcagattgtt cccctttttg aaacaattga
    23761 agacttggat cattcagagg aaacaatgag aaaatatctt tctcttagcc ttgccaaaaa
    23821 atggattgac tcacgaaata actaccaaga aatcatgctt ggctactctg acagtaataa
    23881 agatggcggt tacttgtcat catgttggac cctctacaag gctcaacaac aattgactgc
    23941 tattggagat gaatttggcg ttaaggttac cttcttccat ggtcgtggtg gtactgtcgg
    24001 tcgtggtggt gggccaacct atgaagccat tacatctcaa ccgctcaagt ctatcaagga
    24061 tcgtatccgt ttgacggagc agggtgaagt aattgggaat aaatacggta acaaagacgc
    24121 cgcttactat aaccttgaaa tgctagtatc ggcagctatt aaccgtatga ttactcagaa
    24181 gaagagcgat accaataccc caaatcgtta tgaagccatt atggatcaag tagtggaccg
    24241 tagttacgat atctaccgtg atttggtctt tggtaatgag catttctatg attatttctt
    24301 cgagtcaagt ccaatcaagg ctatttcaag ttttaatatt ggttctcgtc cagccgctcg
    24361 taagactatt actgaaatcg gtggtttgcg tgccatccct tgggtattct catggtcaca
    24421 gagtcgtgtt atgttccctg gatggtacgg ggttggttca agcttcaagg aatttatcaa
    24481 taaaaatcca gagaatattg ctatcttacg agatatgtac caaaattggc ctttcttcca
    24541 atcgcttctt tcaaatgttg atatggtttt gtcaaaatca aatatgaata ttgcttttga
    24601 atatgctaaa ctttgtgaag acgagcaagt taaggccatc tatgagatta ttttaaatga
    24661 atggcaagtt actaagaacg ttatcttggc tattgaagga catgacgaac tcttagctga
    24721 caatccatat ctaaaagcta gtctggatta ccgtatgcct tactttaata ttctcaacta
    24781 tattcagtta gagttgatta aacgccaacg tcgtggagaa ttgtccagtg atcaagaacg
    24841 attgattcat atcaccatca acggaattgc gacaggattg cgtaattcag gttgataatt
    24901 ttcaagagtg aatgctaaaa gtgaatatca aaaaaattct aatagactat tgacaagtag
    24961 tttaaaaatg atataattta accattcaga aaagtaatca tacaaacttt ttagagagtc
    25021 tgtggtagct gaaaacagat aagtggcgat gatgaaaatt gggctgaatg ctatttagaa
    25081 tttgaaatta taaaaattcg gtaagcacac cttacagtgc atctcgttat tgcgagactg
    25141 agcgataggg aaattcccta taattgaggt ggtaccgcgc atcgacgtcc tcacacaagt
    25201 tttttgtgtg aggatttttt tgatggaggt tagtatggaa agaaaacgat ggcgtcgctt
    25261 gtttagataa gtgaaatatg ttaaaggaaa taaaaaggag aaacagaatg aaaaataaac
    25321 gtttaattgg aattattgct gcattagcag tcttagtagc aggaagcttg atttattctt
    25381 caatgaataa atcagaagct cagaataata aggatgagaa gaaaataacc aagattggtg
    25441 tgcttcaatt tgtgagccat ccatcccttg atttgattta taaagggatc caagatggac
    25501 ttgcagaaga aggatataaa gatgatcaag ttaaaattga ttttatgaac tcagaaggtg
    25561 atcaaagtaa ggttgcgaca atgagtaaac aattggttgc aaatgggaat gaccttgtgg
    25621 ttggtattgc aacaccagca gcccaagggt tggctagtgc aacaaaagac ctaccagtta
    25681 tcatggccgc tattacagac ccaattggtg ctaacttggt taaagatttg aaaaaaccag
    25741 gtggtaacgt tacaggggta tctgaccaca atccagctca acaacaaatt gaactcatca
    25801 aggctctgac accgaatgtg aaaacaatcg gagctcttta ctcaagtagc gaagacaatt
    25861 caaaaacaca ggttgaagaa tttaaggctt atgctgaaaa agcaggtctg acagtggaaa
    25921 catttgcagt tccttcaaca aatgaaattg cctcaactgt cactgttatg actatcaagg
    25981 tagatgctat ttgggttcca attgataaca ccattgcatc aggatttcca acggttgtct
    26041 ctagcaatca aagttctaag aaaccaattt atcccagtgc gacagctatg gtagaagtag
    26101 gtggtttggc atcagttgta attgaccaac atgaccttgg tgtggcaaca ggtaaaatga
    26161 ttgtgcaagt cttgaaaggt gcaaaaccag ccgatacccc agtcaatgtc ttttcaactg
    26221 gtaagtcagt catcaataaa aaaatagcac aagaactagg tattactatt cctgagtctg
    26281 ttctcaaaga agcaggacaa gtcatcgaat aaataaggta gggaggagga gactcttccc
    26341 attttttaaa aagatgaata tagaaaggat taaataaaat atgattgttt ccattatttc
    26401 tcaaggattt gtctgggcta ttctaggtct gggaatcttt atgacattta ggattttaaa
    26461 ctttccagat atgacgacag aaggttcctt ccctcttggg ggagctgttg ctgtcacttt
    26521 gataaccaaa ggcgtgaacc catttttagc gacacttgtt gctgtaggag caggttgttt
    26581 ggctggaatg gcagcaggcc ttctttatac aaaagggaag atcccaacct tgctctcagg
    26641 gattttggtg atgacttctt gtcactcaat catgctcttg attatgggac gtgcgaattt
    26701 aggcctgctt ggaaccaagc aaattcagga tgttttgcct tttgattcgg atttgaatca
    26761 actcttgaca ggtctcatct ttgtgagcat tgttattgct ctcatgctct ttttcttgga
    26821 cactaaactc ggacaagcct atattgctac aggggataat cctgatatgg ctagaagttt
    26881 cgggattcat actggacgca tggagctcat gggcttggtc ttatcaaatg gtgtgattgc
    26941 ccttgcaggt gccctcattg ctcagcaaga aggctatgcc gatgtgtctc gagggatcgg
    27001 ggttatcgtt gtggggcttg caagtttgat tattggagaa gttattttca agagtttgag
    27061 cttggcagag cgtttggtta ctatcgttgt aggttctatc gcttatcaat ttttagtgtg
    27121 ggcagttatc gcacttggct ttaatacaag ttaccttcgt ttatacagtg ccttgatttt
    27181 agcagtctgc ctcatgattc caacatttaa gcaaacaatc ttgaaaggag ccaagttaag
    27241 caaatgacag caattgtaga attaaaaaat gcaaccaaaa ttgttaaaaa tggctttgat
    27301 gaagaaaaga ttattttaaa tgatgtttcc ttagaaattt ttgaacggga ctttatcacg
    27361 attttgggcg gaaatggtgc tggaaaatca accctcttta acactatagc agggacctta
    27421 tcactaacta gtggaactat ccgtatttta ggtgaagatc tcactaagtt ttcacccgag
    27481 aagcgtgcca agtacctgtc tcgtgtcttc caagatccaa agatggggac agctccccgt
    27541 atgacggtcg ctgaaaatct tttaatcgcc aagtttcgtg gtgagaagcg tggattgtta
    27601 ccacgacgct tgactagcta taaggatgaa tttcaggcaa ccattgaaaa agtaggaaat
    27661 ggtcttgaga aacacttgaa tacaccgatt gagttcttat caggtggaca aagacaggct
    27721 ttgagtctct tgatggcaac cttgaagcga cctgaattac tcctgttaga tgagcatact
    27781 gctgccctgg atccaaagac tagtgttgct ttgatggaat tgacagatga atttgttaag
    27841 aaagatcagc taacagccct tatgattact catcatatgg aagatgctct caaatacggc
    27901 aatcgcttga ttgtcatgaa agaaggacgg attatccaag atttaaacca agaagaaaaa
    27961 gcaaaaatga aaatctctga ttattatcaa ctctttgaat aaagtaggga aaatgagtga
    28021 aatggtttac tttttttctg aaataaagta tactatataa agtaaactat gataacatgg
    28081 aggtattgtg tatggttgac aaacaagtca ttgaagaaat caaaaacaat gccaacattg
    28141 tggaagtcat aggagatgtg atttctttac aaaaggcagg acggaactat ctagggctct
    28201 gtccttttca tggtgaaaaa acaccttctt tcaacgttgt agaggacaag cagttttacc
    28261 actgttttgg ttgtggtcgc tcaggtgatg tctttaaatt catcgaggag taccaagggg
    28321 ttacctttat ggaggctgtc caaatcttag gtcagcgtgt cgggattgag gttgaaaaac
    28381 cgctttatag tgaacagaag ccagcctcgc ctcaccaagc tctttatgat atgcacgaag
    28441 atgcggctaa attttaccat gctattctca tgacaacgac tatgggcgaa gaggccagaa
    28501 attaccttta tcagcggggt ttgacagatg aagtgcttaa acatttttgg attggtttag
    28561 cacctccaga acgaaactat ctctatcaac gtttgtctga tcagtatcgt gaagaggatt
    28621 tactggattc aggcctgttt tatctctcgg atgccaatca atttgtagac acctttcaca
    28681 atcgcattat gtttcccctg acaaatgacc aaggaaaggt cattgccttc tcaggtcgta
    28741 tctggcaaaa aacggattca caaacttcta agtataaaaa cagccgatcg actgcaattt
    28801 ttaacaaaag ttacgaatta tatcatatgg atagggcaaa aaaatcttct ggaaaaacta
    28861 gtgagattta cctgatggaa ggattcatgg atgttattgc agcctatcgg gctggaatcg
    28921 aaaatgctgt ggcgtcgatg ggaacggcct tgagtcgaga gcatgttgag catctgaaaa
    28981 ggttaaccaa gaaattggtt cttgtttacg atggagataa ggctgggcaa gccgcgatat
    29041 tgaaagcatt ggatgaaatt ggtgatatgc ctgtgcaaat cgttagcatg cctgataact
    29101 tggatcctga tgagtatcta caaaaaaatg gtccagaaga cttggcctat ctattaacaa
    29161 aaactcgtat tagtccgatt gagttctaca ttcatcagta caaacctgaa aacagtgaaa
    29221 atctgcaggc tcagattgag tttattgaaa aaatagctcc cttgattgtt caagaaaagt
    29281 ccatcgctgc tcaaaacagc tatattcata ttttagctga cagtctggcg tcctttgatt
    29341 atacccagat tgagcagatt gttaatgaga gtcgtcaggt gcaaaggcag aatcgcatgg
    29401 aaagaatttc cagaccgacg ccaatcacca tgcctgtcac caagcagtta tcggctatta
    29461 tgagggcaga agcccatcta ctctatcgga tgatggaatc ccctcttgtt ttgaacgatt
    29521 accgtttgcg agaagacttt gcatttgcta cacctgaatt tcaggtctta tatgacttgc
    29581 ttggccagta tggaaatctt cctccagaag ttttagcaga gcagacagag gaagttgaaa
    29641 gagcttggta ccaagtttta gctcaggatt tgcctgctga gatatcgccg caggaactta
    29701 gtgaagtaga gatgactcga aacaaggctc tcttgaatca ggacaatatg agaatcaaaa
    29761 agaaggtgca ggaagctagc catgtaggag atacagatac agccctagaa gaattggaac
    29821 gtttaatttc ccaaaagaga agaatggagt aataatggca acaaaacaaa aagaagtaac
    29881 aacatttgac gtacaggtag cagaatttat ccgtaatcat aagcaaaaag ggacagcaac
    29941 agatgatgaa atcaatgcaa gtctggttat tccttttacc ttggacgctg atgggattga
    30001 agatctcttg caacggattc aggatgcagg gatttctatc acagataacg aaggaaatcc
    30061 aagtgcgcgt gttcttagca atgaagaaga accagaactc agcgatgagg acttgattgg
    30121 atcaacttct gctaaggtca atgaccctgt ccgtatgtac ttgaaagaaa taggggtcgt
    30181 tcctctcttg accaatgaag aggagaaaga gttggcactg gctgttgaag ctggcgatat
    30241 cgaagccaaa caacgtcttg cggaagccaa tcttcgtttg gttgtttcca ttgccaaacg
    30301 ctatgtcggt cgtggcatgc agttccttga cttgattcaa gaaggaaata tgggcttgat
    30361 gaaggcggtt gacaagtttg actattctaa agggttcaag ttttcaactt atgcaacttg
    30421 gtggattcgt caggctatca ctcgtgctat tgcggaccaa gctcgtacca tccgtatccc
    30481 agttcacatg gttgaaacta tcaataaatt ggttcgtgaa cagcggaatc tccttcaaga
    30541 attggggcaa gatccgacac cagaacagat tgctgaacga atggatatga cacctgataa
    30601 ggttcgtgaa atcttgaaga ttgcccaaga accagtatct cttgaaactc ctatcggtga
    30661 agaggacgat agccaccttg gagactttat cgaagatgaa gtgattgaaa atccagtgga
    30721 ttatacgact cgtatcgtct tgcgtgagca attggatgaa atcttagata ctcttacaga
    30781 ccgtgaagaa aatgttctgc gtctacgttt tggactagat gatggaaaaa tgcgcacact
    30841 tgaagatgtg gggaaagtct ttaacgtaac tcgtgagcgt atccgtcaga ttgaagcaaa
    30901 ggctttgaga aaactacgcc aaccaagtcg cagcaaaccg cttcgtgatt ttattgaaga
    30961 ctaagagtga ggaaaatatg gcttatacag aagagcaaat tgaaaacatc aaaacacgga
    31021 ttttaacagc cttggaagaa gtcatcgacc ctgagttggg aatcgatatt gtcaatcttg
    31081 gtttgatcta tgagattcgt tttgacggtg acacagggca aacagagatt gacatgactt
    31141 tgacaactat gggttgtccc ttagcagacc ttttgacaga ccagatttat gatgctatga
    31201 tagaggttcc agaagtaacg gatactgagg ttaaattagt ctggtatcca gcctggacag
    31261 ttgaaaaaat gagtcgctat gcccgcattg ccctaggcat taagtaaaca tataaaagca
    31321 tgtgagagac tagaggaaaa gcgaggaaat ttcccctctt ttcctctagt ctctcctttc
    31381 ttttgctgat tttattcaaa gaaaatgata taatagtagt tatggagaaa aagaaattac
    31441 gcatcaatat gttgagttca agtgagaaag tagcaggaca gggagtttca ggtgcttacc
    31501 gtgaattagt tcgtcttctt caccgtgctg ccaaggacca attgattgtt acagaaaatc
    31561 ttccaatcga ggcagatgtg actcactttc atacgattga ttttccctat tatttatcaa
    31621 ccttccaaaa gaaacgctca gggagaaaga ttggctatgt gcatttcttg ccagctacac
    31681 ttgaaggaag tttgaaaatt ccatttttct taaagggaat tgtgaaacgc tatgtatttt
    31741 ctttttacaa ccggatggag cacttggttg tggtcaatcc tatgtttatt gaggatttgg
    31801 tagcagctgg tattccacgt gaaaaagtga cctatattcc taactttgtc aacaaggaaa
    31861 aatggcatcc tctaccacaa gaagaggtag tcagactgcg cacagatctt ggtcttagtg
    31921 acaatcagtt tatcgtagta ggtgctgggc aagttcagaa acgtaaaggg attgatgact
    31981 ttatccgtct ggctgaggaa ttgcctcaga ttacctttat ctgggctggt ggcttctctt
    32041 ttggtggtat gacagatggt tatgaacact ataagaaaat tatggaaaat ccccctaaaa
    32101 atttgatttt tccaggcatt gtatcgccag agcggatgcg cgaattgtat gctctagcgg
    32161 atcttttctt gttgcctagt tacaatgagc tctttcctat gactatttta gaagctgcga
    32221 gttgtgaggc tcctattatg ttgcgtgatt tagatctcta taaggtgatt ttggagggaa
    32281 attatcgggc gacagcgggt agagaagaga tgaaagaggc tattttggaa tatcaagcaa
    32341 atcctgctgt cttaaaagat ctcaaagaaa aggctaagaa tatttccaga gagtattctg
    32401 aagagcatct gttacaaatc tggttggact tttatgagaa acaagccgct ttagggagaa
    32461 agtaaaaagt gaggtaatct atgcgaattg gtttatttac agatacctat tttcctcagg
    32521 tttctggtgt tgcgaccagt attcgaacct tgaaaacaga acttgaaaag cagggacatg
    32581 ctgtttttat ctttacgacg acagataagg atgtcaatcg ctacgaagat tggcaaatta
    32641 tccgcattcc aagtgttcct ttctttgctt ttaaggatcg tcgctttgcc taccgaggtt
    32701 ttagcaaggc acttgaaatt gctaaacagt atcagctaga tattatccat actcagacag
    32761 aattttctct tggcctgttg gggatttgga ttgcgcgtga attgaaaatt ccagtcatcc
    32821 atacctatca cacccagtat gaagactatg tccattatat tgctaagggg atattgatcc
    32881 ggccgagtat ggtcaagtat ctggttagag gtttcctgca tgatgtggat ggggttattt
    32941 gccctagtga gattgtccgt gacttgctat ctgattataa ggtcaaggtt gaaaaacggg
    33001 tcattcctac tgggattgaa ttagccaagt ttgagcgtcc ggaaatcaag caggaaaatt
    33061 tgaaagaact gcgtagtaaa ctagggattc aagatggtga aaagacgttg cttagtcttt
    33121 cgagaatctc ctatgaaaaa aatattcaag cagttttagc agcctttgct gatgttctga
    33181 aagaggaaga caaggttaaa ctggtagtag ctggggatgg cccttatctg aatgacctca
    33241 aagagcaagc ccagaaccta gagattcaag actcagtcat ctttacaggg atgattgctc
    33301 ctagtgagac ggctctttac tataaagcgg cggatttctt catttcggca tcgacaagcg
    33361 aaacgcaagg tttgacctac ttggaaagct tagccagtgg aacacctgtc attgctcacg
    33421 gaaatcctta tttgaacaac ctcatcagtg ataaaatgtt tggagccttg tactatggag
    33481 aacatgattt ggctggtgct attttggaag ccctgattgc aacaccagac atgaatgagc
    33541 ataccttatc agagaaattg tatgagattt cagctgagaa ctttgggaaa cgagtgcatg
    33601 agttttatct ggatgccatt atttcaaata acttccagaa agatttggct aaagatgata
    33661 cggtcagtca gcgtatcttt aggacagttt tgtatcttcc gcaacaggtg gttgctgtac
    33721 ctgtaaaagg atctagacgc atgttgaagg cttcaaaaac acagttgatc agtatgagag
    33781 actattggaa agaccatgaa gaatagaaag aggaacagct atgaaaaaaa caattaatga
    33841 gaagcggtcg tgataaaaag attgcgggtg tttgtgctgg ggtggcccat tatctggata
    33901 tggatccgac tatcgttcaa gtcatttggg gtgttcttac ttgctgttac ggagctggaa
    33961 ttgtagctta cattatttta tggattatcg caccagtagc aactgactat tgaaaactag
    34021 ctcaattcat gctaagataa tagaaaatag aatgtaacga aggagagaaa aatggcattt
    34081 ggagataatg gaaatcgtaa aaaaactatg tttgagaaaa taaccttgtt tatcgtgatt
    34141 atcatgctag tagcaagttt attgggaatt tttgcaactg caattggtgc cctcagtaat
    34201 ctataaaata gattcaagaa aatttagtga ctgggatttc ccagcccttt tttaaagtga
    34261 gaagaaataa tgagtatgtt tttagataca gctaagatta aggtcaaggc tggtaatggt
    34321 ggcgatggta tggttgcctt tcgtcgtgaa aaatatgtcc ctaatggagg cccttggggt
    34381 ggtgatggtg gtcgtggagg caatgtggtc ttcgttgtag acgaaggact acgtaccttg
    34441 atggatttcc gctacaatcg tcatttcaag gctgattctg gtgaaaaagg gatgaccaaa
    34501 gggatgcatg gtcgtggtgc tgaggacctt agagttcgag taccacaagg tacgactgtt
    34561 cgtgatgcgg agactggcaa ggttttaaca gatttgattg aacatgggca agaatttatc
    34621 gttgcccacg gtggtcgtgg tggacgtgga aatattcgtt tcgcgacacc aaaaaatcct
    34681 gcaccggaaa tctctgaaaa tggagaacca ggtcaggaac gtgagttaca attggaacta
    34741 aaaatcttgg cagatgtcgg tttagtagga ttcccatctg tagggaagtc aacactttta
    34801 agtgttatta cctcagctaa gcctaaaatt ggtgcctacc actttaccac tattgtacca
    34861 aatttaggta tggttcgcac ccaatcaggt gaatcctttg cagtagccga cttgccaggt
    34921 ttgattgaag gggctagtca aggtgttggt ttgggaactc agtttctccg tcacatcgag
    34981 cgtacacgtg ttatccttca catcattgat atgtcagcta gcgaaggccg tgatccatat
    35041 gaggattacc tagctatcaa taaagagctg gagtcttaca atcttcgcct catggagcgt
    35101 ccacagatta ttgtagctaa taagatggac atgcctgaga gtcaggaaaa tcttgaagaa
    35161 tttaagaaaa aattgtctga aaattatgat gaatttgaag agttaccagc tatcttccca
    35221 atttctggat tgaccaagca aggtctggca acacttttag atgctacagc tgaattgtta
    35281 gacaagacac cagaattttt gctctacgac gagtccgata tggaagaaga agcttactat
    35341 ggatttgacg aagaagaaaa agcctttgaa attagtcgtg atgacgatgc gacatgggta
    35401 ctttctggtg aaaaactcat gaaactcttt aatatgacca actttgatcg tgatgaatct
    35461 gtcatgaaat ttgcccgtca gcttcgtggt atgggggttg atgaagccct tcgtgcgcgt
    35521 ggagctaaag atggggattt ggtccgcatt ggtaaatttg agtttgaatt tgtagactag
    35581 gagactggta tgggagataa accgatatct ttccgagatg cggatggtaa ttttgtttcc
    35641 gccgcagacg tttggaatga aaagaaattg gaagaactat ttaatcgtct caatccaaat
    35701 cgtgccttga gattggcacg aactaaaaag gaaaatccat ctcagtaaag aagctaaaaa
    35761 atcccgtgcc tcatcagaca cgggattttg tggtacgaca ggcatgtata gcaaactgaa
    35821 tctggaatag cacagcatat cttctaaaat attgttataa attgatttga atttcaaaat
    35881 taaattgttt gattcttatc tcaatttgtt atagtatatc tgatgtcaaa gttctcggcg
    35941 agtcaaatag cgattcccaa gcctgactat cgtgaggtag cggattaaaa tggtctgggg
    36001 atagaccgtt ttaagtctga cgctggaaat aagaattgtc agaagaaggg atagcgaaat
    36061 cgtggctcta cgaacaggaa cgtgataata aggcgtatat agcggataag agggcctcaa
    36121 actctaaagt ccaaaaaggt agtcgtaacc tatatgcgta aatcacgaga gtaattgaat
    36181 tcgtactaag attttctatt ttcactgtaa ccttttaacg tccttatatc ttgtatacac
    36241 gaggaaagat gtacgactta tcccgtgagg tctatcacta taaagagaaa acgacagata
    36301 gaagtgatcc tgagtcacgg ttatctttaa aatagattgt ctgataggac ggtatgtata
    36361 aaacgcttct gtgaactgag agaaggggga gaagttcttg ctaaaattta gttgaacagc
    36421 cgtattccga tacttagata agagatctag tcttagctcc tactcagttt taggggataa
    36481 aaaaggggaa atagcgattc gagaaagatt atactcttcg aaaatctctt caaatcacgt
    36541 caatatcgcc ttgccgtatg tgtaggatac tgactacgtc agttccatct acaacctcaa
    36601 aacagtgttt tgagcaacct gcggctagtt tcctagtttg atctttgatt ttcattgagt
    36661 attagtaatt cagttactaa ctcgtcaact ctgatttatc caataaaatt gaaaaggatg
    36721 gaaaaaagga taaatttatg atatacttta ttttgaagac cttattagaa atcttgaaag
    36781 agtattgaaa acttagaatg agaaaaattg ttatcaatgg tggattacca ctgcaaggtg
    36841 aaatcactat tagtggtgct aaaaatagtg tcgttgcctt aattccagct attatcttgg
    36901 ctgatgatgt ggtgactttg gattgcgttc cagatatttc ggatgtagcc agtcttgtcg
    36961 aaatcatgga attgatggga gctactgtta agcgttatga cgatgtattg gagattgacc
    37021 caagaggtgt tcaaaatatt ccaatgcctt atggtaaaat taacagtctt cgtgcatctt
    37081 actattttta tgggagcctc ttaggccgtt ttggtgaagc gacagttggt ctaccgggag
    37141 gatgtgatct tggtcctcgt ccgattgact tacaccttaa ggcgtttgaa gctatgggtg
    37201 ccactgctag ctacgaggga gataacatga agttatctgc taaagataca ggacttcatg
    37261 gtgcaagtat ttacatggat acggttagtg tgggagcaac gattaatacg atgattgctg
    37321 cggttaaagc aaatggtcgt actattattg aaaatgcagc ccgtgaacct gagattattg
    37381 atgtagctac tctcttgaat aatatgggtg cccatatccg tggggcagga actaatatca
    37441 tcattattga tggtgttgaa agattacatg ggacacgtca tcaggtgatt ccagaccgta
    37501 ttgaagctgg aacatatata tctttagctg ctgcagttgg taaaggaatt cgtataaata
    37561 atgttcttta cgaacacctg gaagggttta ttgctaagtt ggaagaaatg ggagtgagaa
    37621 tgactgtatc tgaggacagc atttttgtcg aggaacagtc taatttgaaa gcaatcaata
    37681 ttaagacagc tccttaccca ggctttgcaa ctgatttgca acaaccgctt acccctcttt
    37741 tactaagagc gaatggtcgt ggtacaattg tcgatacgat ttacgaaaaa cgtgtaaatc
    37801 atgtttttga actagcaaag atggatgcgg atatttcgac aacaaatggt catattttgt
    37861 acacgggtgg acgtgattta cgtggggcca gtgttaaagc gaccgactta agagctgggg
    37921 ctgcactagt cattgctggg cttatggctg aaggtaaaac tgaaattacc aatatcgagt
    37981 ttatcttacg tggttattct gatattatcg aaaaattacg taatttagga gcggatatta
    38041 gacttgttga ggattaaacc gtagaggtgt ttatgaatat ttggaccaaa ttagcaatgt
    38101 tttctttttt tgaaacggat cgcttgtatt tgcgtccttt cttttttagt gatagtcagg
    38161 acttccgcga gatagcttca aatccagaaa atcttcaatt tattttccca acgcaggcaa
    38221 gtctggaaga aagtcaatat gcactggcca attactttat gaagtcccct ttgggagtgt
    38281 gggcaatttg tgaccagaaa aatcaacaaa tgattggttc tattaaattt gagaagttag
    38341 atgaaatcaa aaaagaagct gagcttggct attttttgag aaaagatgct tggtcgcaag
    38401 gatttatgac agaggttgtt agaaaaattt gtcagctttc ttttgaggaa tttggcttaa
    38461 aacaattatt tatcattacc caccttgaaa atgaagctag ccaaagagtt gctcttaagt
    38521 ctggatttag tttgttccgt cagtttaagg gaagtgatcg ttacacaaga aaaatgcggg
    38581 attatcttga atttcggtat gtaaaaggag agttcaatga gtaagcatca ggaaattcta
    38641 agctatttgg aggaattacc agtaggtaaa agggtcagtg ttcgtagcat ttcgaatcat
    38701 ctaggagtta gtgatggaac agcctatcga gctattaaag aagctgaaaa ccgtggaatt
    38761 gtggagaccc gtcctagaag tggaacaatt cgtgttaaat cccagaaagt tgctatagat
    38821 agattaacgt ttgctgaaat tgcagaagtg acttcttctg aggttctggc tgggcaagaa
    38881 ggtttagaga gagaatttag taagttttca attggtgcca tgactgaaca aaatatcttg
    38941 tcttaccttc atgatggggg gctcttgatt gtcggagacc gaacccgtat tcagttgcta
    39001 gccttggaaa atgaaaatgc agttctggtt acagggggat ttcaggttca tgatgatgtg
    39061 cttaaactgg ccaatcaaaa agggattcct gttctaagaa gtaagcatga tacctttacc
    39121 gtcgctacca tgatcaataa agccttgtca aatgtccaaa tcaagactga tattctgaca
    39181 gttgagaaac tttatcgccc tagtcatgag tatggttttc tgagagagac agatacagtt
    39241 aaagattatt tggacttggt tcgtaagaat cgtagcagcc gtttccctgt tatcaatcaa
    39301 catcaggtcg ttgttggcgt tgtaaccatg agagacgctg gtgataaatc accaagcacg
    39361 acaattgata aggttatgtc tcgtagtcta tttttggttg gattatcgac aaatattgcc
    39421 aatgtgagtc aacggatgat cgcagaagac tttgaaatgg taccagttgt tcgaagcaat
    39481 caaactttgc ttggcgttgt gacgcgacga gatgtcatgg agaagatgag ccgttcccaa
    39541 gtttcggctc taccaacttt ttctgagcag attggacaaa agctctctta tcaccatgat
    39601 gaagtagtca ttacagtgga accctttatg ctagaaaaaa atggagtttt ggctaatggt
    39661 gtattggcag aaattctgac ccacatgacc caagatttag ttgttaatag tggtcgcaat
    39721 ctcattatcg agcagatgct gatctacttt ttgcaggccg ttcagataga tgatatattg
    39781 cgcattcagg cacggattat tcatcatacg agacggtcag ctataattga ttacgatatt
    39841 tatcatggtc accagattgt ttcaaaagca aatgtgactg ttaaaattaa ttagaaacta
    39901 ggagaaaaga tgataacatt aaaatcagct cgtgaaatcg aagctatgga caaggctggt
    39961 gattttctag caagtattca tataggctta cgtgatttga ttaagccagg cgtagatatg
    40021 tgggaagttg aagaatatgt ccgccgtcgt tgtaaagaag aaaatttcct tccacttcag
    40081 attggggttg acggtgccat gatggactat ccttatgcta cctgttgctc tcttaacgat
    40141 gaagtggctc acgctttccc tcgtcattat atcttgaaag atggtgattt gctcaaagtt
    40201 gatatggttt tgggaggtcc cattgctaaa tctgacctaa atgtctcaaa attaaacttc
    40261 aacaatgttg aacaaatgaa aaaatacact cagagttatt ctggtggttt agcagactca
    40321 tgttgggctt atgctgttgg tacaccgtcc gaagaagtca aaaacttgat ggatgtaacc
    40381 aaagaagcta tgtacaaggg tattgagcaa gctgttgttg gaaatcgtat cggtgatatc
    40441 ggtgcggcta ttcaagaata cgctgaaagt cgtggttacg gtgtagtgcg tgatttggtt
    40501 ggtcatggtg ttggcccaac tatgcacgaa gaaccaatgg ttcctaacta tggtattgca
    40561 ggtcgtggac tccgtcttcg tgaaggaatg gtcttaacca ttgaaccaat gatcaataca
    40621 ggcgattggg aaattgatac agatatgaaa actggttggg cgcataagac cattgacggt
    40681 ggattgtcat gtcagtatga acaccaattt gtcattacga aagatggacc tgttatcttg
    40741 actagccaag gtgaagaagg aacttattaa taaaaagtga aaagactact ggaagtttat
    40801 tatgaaaaaa tccagtagat cttttcataa taaaacgcat tgtatcaagt gttaggggct
    40861 gatatcatgc gtttttctgc ttttaagatt ttttccaact ctgtttgtaa gcgcatcata
    40921 acaaagggtc taggattcag ggctctcctc ctatatacta ttagtaaagt aaaactaagg
    40981 gaggatattt tagtgtcgca gtctattgtt cctgtagaga ttccacaata ttgtcgtttt
    41041 gattctaaaa agagaaatgg aattctgttt aatgttcgta ttgccaatct taaatttacc
    41101 ttttttagat tagacttcct gcgaaacaaa acatgg
//
LOCUS       DAVBCN010000009        36821 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVBCN010000009 DAVBCN010000000
VERSION     DAVBCN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36821
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.009"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(15..311)
                     /locus_tag="VQ497_000456"
     CDS             complement(15..311)
                     /locus_tag="VQ497_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516813.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYSKMK"
     gene            complement(295..2454)
                     /gene="pfbA"
                     /locus_tag="VQ497_000457"
     CDS             complement(295..2454)
                     /gene="pfbA"
                     /locus_tag="VQ497_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HET2516814.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVISTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKESVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            complement(3584..4810)
                     /locus_tag="VQ497_000458"
     CDS             complement(3584..4810)
                     /locus_tag="VQ497_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HET2516815.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVFEVGPGDEVIVPAITYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(4853..5545)
                     /locus_tag="VQ497_000459"
     CDS             complement(4853..5545)
                     /locus_tag="VQ497_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET2516816.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGNLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSTEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFKVLK"
     gene            complement(5731..7482)
                     /locus_tag="VQ497_000460"
     CDS             complement(5731..7482)
                     /locus_tag="VQ497_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2516817.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYRLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALVDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(7472..9217)
                     /locus_tag="VQ497_000461"
     CDS             complement(7472..9217)
                     /locus_tag="VQ497_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2516818.1"
                     /translation="MSIIQKLWWFFKLEKHRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSPAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGYDIRDYRLTELRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSEVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILNPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGRHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(9423..9719)
                     /locus_tag="VQ497_000462"
     CDS             complement(9423..9719)
                     /locus_tag="VQ497_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HET2516819.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(9872..10328)
                     /locus_tag="VQ497_000463"
                     /pseudo
     CDS             complement(9872..10328)
                     /locus_tag="VQ497_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            10462..11289
                     /gene="xth"
                     /locus_tag="VQ497_000464"
     CDS             10462..11289
                     /gene="xth"
                     /locus_tag="VQ497_000464"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET2516820.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            11301..11444
                     /locus_tag="VQ497_000465"
     CDS             11301..11444
                     /locus_tag="VQ497_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET2516821.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEYQRSEENFLFLLK
                     SVN"
     gene            11444..11683
                     /locus_tag="VQ497_000466"
     CDS             11444..11683
                     /locus_tag="VQ497_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET2516822.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRPLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      12067..12162
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            12562..13143
                     /locus_tag="VQ497_000467"
     CDS             12562..13143
                     /locus_tag="VQ497_000467"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HET2516823.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            13143..14405
                     /locus_tag="VQ497_000468"
     CDS             13143..14405
                     /locus_tag="VQ497_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HET2516824.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LSAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(14492..15358)
                     /locus_tag="VQ497_000469"
     CDS             complement(14492..15358)
                     /locus_tag="VQ497_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HET2516825.1"
                     /translation="MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIM
                     NSLIGSKNIQEDFLDLYQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFYFDFSKRNYS
                     IEEYTMFLEKSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLE
                     AEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFY
                     SGSGSKLNETARSYKMIAEEIKAIPNVEFMWITDGQGWYKAKNNLRETFDILPFLYNI
                     NDLEHNILKNLK"
     gene            complement(15345..16115)
                     /locus_tag="VQ497_000470"
     CDS             complement(15345..16115)
                     /locus_tag="VQ497_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET2516826.1"
                     /translation="MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNG
                     GISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYS
                     VGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
                     KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGS
                     GTTGVVAKRLGRRFIGIDAEKEYLKIARKRLEAENETN"
     gene            complement(16141..16995)
                     /locus_tag="VQ497_000471"
     CDS             complement(16141..16995)
                     /locus_tag="VQ497_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HET2516827.1"
                     /translation="MKIKEIKKVTLQPFTKWTGGKRQLLPVIRELMPKTYNRYFEPFV
                     GGGALFFDLAPKDAVINDFNAELINCYQQIKDNPQELIEILKVHQEYNSKEYYLDLRS
                     ADRDERIDMMSEVQRAARILYMLRVNFNGLYRVNSKNQFNVPYGRYKNPKIVDEELIS
                     AISVYLNNNQLEIKVGDFEKAIVDVRTGDFVYFDPPYIPLSETSAFTSYTHEGFSFAD
                     QVRLRDAFKRLSDTGAYVMLSNSSSALVEELYKDFNIHYVEATRTNGAKSSSRGKISE
                     IIVTNYEK"
     gene            complement(17152..17556)
                     /locus_tag="VQ497_000472"
     CDS             complement(17152..17556)
                     /locus_tag="VQ497_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET2516828.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVRI"
     gene            complement(17626..19107)
                     /locus_tag="VQ497_000473"
     CDS             complement(17626..19107)
                     /locus_tag="VQ497_000473"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HET2516829.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFARVLEDAGVYKQTEQGQTAFMRFVEHVGILLD"
     gene            complement(19126..20304)
                     /locus_tag="VQ497_000474"
     CDS             complement(19126..20304)
                     /locus_tag="VQ497_000474"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HET2516830.1"
                     /translation="MAQHLTTEALRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVFENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            20469..21476
                     /gene="galR"
                     /locus_tag="VQ497_000475"
     CDS             20469..21476
                     /gene="galR"
                     /locus_tag="VQ497_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HET2516831.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFRNYSQAKGIYHDELVFQGN
                     FTAQSGYDLMKEAIQNLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHDRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(21522..22559)
                     /locus_tag="VQ497_000476"
     CDS             complement(21522..22559)
                     /locus_tag="VQ497_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET2516832.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEVDDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATVVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(22560..22913)
                     /locus_tag="VQ497_000477"
     CDS             complement(22560..22913)
                     /locus_tag="VQ497_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET2516833.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(23157..24047)
                     /locus_tag="VQ497_000478"
     CDS             complement(23157..24047)
                     /locus_tag="VQ497_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET2516834.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            24180..24716
                     /locus_tag="VQ497_000479"
     CDS             24180..24716
                     /locus_tag="VQ497_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET2516835.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFQKNQDHITSLLFS
                     KNDYFLRQLQRELEHHVYPMLAADLKQTHPSLPTSYLQHLVVSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(24883..25689)
                     /locus_tag="VQ497_000480"
     CDS             complement(24883..25689)
                     /locus_tag="VQ497_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HET2516836.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(26034..27554)
                     /locus_tag="VQ497_000481"
     CDS             complement(26034..27554)
                     /locus_tag="VQ497_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HET2516837.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(27547..28275)
                     /locus_tag="VQ497_000482"
     CDS             complement(27547..28275)
                     /locus_tag="VQ497_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2516838.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            complement(28290..29054)
                     /locus_tag="VQ497_000483"
     CDS             complement(28290..29054)
                     /locus_tag="VQ497_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET2516839.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFREDENG
                     CIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(29058..29501)
                     /locus_tag="VQ497_000484"
     CDS             complement(29058..29501)
                     /locus_tag="VQ497_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2516840.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKFQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(30183..30479)
                     /locus_tag="VQ497_000485"
     CDS             complement(30183..30479)
                     /locus_tag="VQ497_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET2516841.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETAKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(30501..31565)
                     /gene="pepA"
                     /locus_tag="VQ497_000486"
     CDS             complement(30501..31565)
                     /gene="pepA"
                     /locus_tag="VQ497_000486"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HET2516842.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            31966..32667
                     /gene="galE"
                     /locus_tag="VQ497_000487"
     CDS             31966..32667
                     /gene="galE"
                     /locus_tag="VQ497_000487"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET2516843.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            33090..34049
                     /locus_tag="VQ497_000488"
     CDS             33090..34049
                     /locus_tag="VQ497_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2516844.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKEISESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLGFILLLWRGGRKDAD"
     gene            34039..34995
                     /locus_tag="VQ497_000489"
     CDS             34039..34995
                     /locus_tag="VQ497_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HET2516845.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLTLQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            34992..35744
                     /locus_tag="VQ497_000490"
     CDS             34992..35744
                     /locus_tag="VQ497_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2516846.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGS
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            35840..36805
                     /locus_tag="VQ497_000491"
     CDS             35840..36805
                     /locus_tag="VQ497_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2516847.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPAILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
BASE COUNT        10928 a         8282 c         6621 g        10990 t
ORIGIN      
        1 gatttgtcct attattattt cattttacta tactctgtta attcatatga gtttaaaccg
       61 atttcatctt taacctcgag taaagcagtt tcaaatattt gtttaagagt ttttgattct
      121 ttacaattaa ccgacaaact ttctgataaa atatgtacaa cttctgagac tgaataacct
      181 atctcctctt tagaattata taaatctgta gctccaccaa taatccaaaa atactgattt
      241 tgtgaaccta caatatcctc attttctacg gaacttcctt gtatcgaaca aattttattt
      301 atctttacca taatacttca acccttttag tgtcaaaagt aaaccaattc ctgtcactgt
      361 taagaatagt tccataatct tgttcgaacc agtctttggt aatttttgtt ttacatctac
      421 tatttcttta gatttattaa tatgattttc agtttctctg ccatctccaa ctattttata
      481 gtttacttct tctgtcttat tatcttgttt attgtcgatc ttgtcattca tttgtctatt
      541 atctttactt gagttaaact ctccgttctt ctggttacta tcaattacat tatttgaatt
      601 agattgtttt tcctctttgt ttttttcttt ttcgttttta tcacttaaat tatttgttac
      661 aattttgtaa agcccattct ccgttacaat attgaaatta ccatcgctat cacgtataac
      721 agattctttc ccatttgcat tagatttgat gaatgatata tacttaccgg ataaattata
      781 aaattggtta tttaaaacgg ttattttacc ctttgaatcc tcaataacaa ttccttcttt
      841 accaccgaat atactattct ctgagactac taaattatca ctaactcgta ataattcaat
      901 atttggttgt tctgtttcct tagaattatt attaattaca ttttttgtta cagtaatatc
      961 tgatactttt cctaaatatt ctgcactatc ttttgcaact cgtatcgctt ttgttttagg
     1021 atcggcaata ttaaatatat tttcggcgat aaccacctgt ttatttaaat ctaataggtc
     1081 tttagtgttt ttatagctat aagcatttac taaagctgct ccgctttctc gataatgtac
     1141 actctctcct ttaactttct tatcaaagcg atttcctttg attaatacat cagtgaatcc
     1201 tgtaaaacgt acacctgcat acatcatgtt atcaaaatga ttatttaaaa ttttaatatt
     1261 agaggggttc tgtgtcgaca atgtttgata gtgtgtgcca attgctgtta ctaattcccc
     1321 agatttatca cttttgccaa aataggaatt ttgaatagtc acattttcag attttttccc
     1381 atcatcattc aaggcataag gaaaaccttt tctagttaat ggttcaatct gaatgctctc
     1441 cttacttatg atttgcccat ccttcatcgt tttgggtaag gcttgcccaa gaaaacgaga
     1501 attatcaact aatacatttt tcgaacctgc aatttgaata gcatgccctt gataactatc
     1561 cttgaatgtt acatttttta tagttacgtt atttgaattc ccaatagcaa atgcacctga
     1621 agaatttata agtggtagat tttttgcttt agttccttct tcattcaaag caccgttcat
     1681 atcaatcgta ccaccagaat aactaatatc ttctgttggg ccccattcta cttgcgcacc
     1741 atcatccgta aataaacctg tcataaaaac aatggaaggg tgattcttaa tatttatacc
     1801 atttagaatt gtagctttct cattcaattc taagtgtgta tgacttttta aaaaaacaat
     1861 ttcttttact aaataagttc cttcaggaaa atatacattt cctccaccta gcccttgagc
     1921 tgcagcatct attgcatctt gaattgcttg tcgatcatca tgaatcccat cacctaccgc
     1981 accataatct tttacactta aagcagtatc ttcaaattct tttttgataa ttctgtcact
     2041 tataacatct tccttataac tcttattttc attagtagat attactgtag attcaactgt
     2101 tctgttgtct aattgtgaat tggaacgaat agtaccatta gagctgatac tattaacact
     2161 taacgaatta tctaaaccat tagtggttga attagaggca atcatttcag cagaacgatt
     2221 ttgattaaca gttggtgaat tatctaaatc attagtgatt gcattagaag actcttttgt
     2281 agccatcggt gaagaactag taacaacttc atccgataaa ataccttggc ttcccaaaat
     2341 tgaaattgcc aatagtaccg agcaagtccc cacctttaat ttacgaatac tgaatacctc
     2401 attaggaaca aaatatttca tcagaacctc caattttttt actattttta acaattgatt
     2461 attacttttc ttaaaataga tagaatgaca ccgctgtcat tatctttatc atatattttc
     2521 caaattgaat tgtcaaggaa taacacaaga aaatattaaa atttatactc ttcggaaatc
     2581 tcttcaaact aggtcagtgc cgccttgccc taagtctagg ttactgactt ggtcagttct
     2641 atagtagact gaaactataa tagtacacct ctacttctaa aacattgtta gaaatcgatt
     2701 tgactgtccc gatcgatttg tcctattctt atttcatttt attatatcta caacctcaaa
     2761 gcagggtttt gagctatcta tggccaactt cctagtttgc tctttagcct tccattcaaa
     2821 agtgatgcta tctttcttag cttgaatata aaatatccca tttactatat ttaaatttgt
     2881 tgatatgttg taaattataa atgaaagaac aaatatcttg tcatacaagc attcgccagg
     2941 gatatttaca tgaatatcgc tcatatggcg gattgaaact aaaacactac aatatatttc
     3001 ttaaatagac cttctataaa agctagattt aacctcgata aaaatagatg cagtgcttct
     3061 aaaatactgg aatagggcat ataggtaatc gtagacaagt gttttataga ttgtctcgcg
     3121 ggagatctaa tgatgtatag caagcatcac taaacatata gaatatttgt gttattataa
     3181 tgtcaatatg ctataaattc tatcatgcaa aagaatgact agaacatgaa agacaggcac
     3241 aaaactttga acaatattct gaaaacaaaa aaagacgcga gtttccacaa tctcactcat
     3301 ggaaactcac gtctttcaaa aaatagggtg aattaaaaac tagaacaaga cactagattt
     3361 tctaaatgat gtctagtaag gattctaaat gctacaccaa attgctcaca tcttatttca
     3421 aaccacggga ctcccacaag aaaggaaaat cctataaaaa ttctctctct gaggagagag
     3481 aggcctcaac tacaggtgta aactattatc aattgaaaac aacagttttt acactcaatt
     3541 agacttttta gggacaggat cactttcgct tgactgtagt ttgtcatttt tttgataaag
     3601 ttagcacttt ttcagaaact gttttgaaag tctcaatgat atagtctact tcttcatcgc
     3661 ttaatttagt atgaagaggg agggtaattt cattctcaaa gaaggcatag gccttaggat
     3721 agttcgtcat atcaaatcca agattcttat aggctgtcaa gagaggaagc ggtttgtagt
     3781 gaacattact tgcaattcct gctttagcca attcttggat gatgaggttg cgttcttcta
     3841 ggcttgctcc ttctacacgg gtgatgtaga ggtggcgtga agattcgaca gtttcagtct
     3901 tgtgtgccaa aggatggatg cgagaacctg caaaaccact atcatagcgg tccacaatgt
     3961 ccttacggcg ttgcaacaaa cttggatagc ggtccaattg taccaaacca agtgaagcca
     4021 tgatatcggt catgttgcac ttataggctg gtgtaacgat atcgtattcc catgacccca
     4081 gttgcatctt ggcaagagca tccttagttt gcccgtgaag ggaaaggatt tggaattcct
     4141 tgtacatctc ttcgtcatca atcactggat tggctttcca agtcgcactt ccaccttctg
     4201 ccgttgtaaa gttcttaacg gcatggaatg agaaggaagt aaagtcagcg atagaaccag
     4261 aaggttgtcc tttataagta gatcccaaag cgtgggcact atcagagaca atgacaatac
     4321 ggttaaaggc cttttgccac ttgcttgaag cggtaaagaa gtcacgtttt ttctccacga
     4381 cttggaacaa acggtcataa tcgcaaacaa tccctgcgag ctctactggg ataatcacct
     4441 tagttttctc agtgatagct tgctcaagca ggtcatagtc catctcaaac gtatctgctt
     4501 ggatatccac catgacaggg gttgctccca cgtgcgtaat gacactacat gaagccgtat
     4561 aggttatggc tggaacgatg acttcatcac caggtcccac ttcaaaaacg cgtaaaatca
     4621 actccagagc ggctgtcgca gagttgagac aaacagtctt aggtgtctgt gtgtaaagag
     4681 acaagcggcg ctccagttct tttgttttag gacctgttgt gatccaacca gaacgcaggg
     4741 tatccgctac ttcagcaatt tctgcttctg tgatatcagg cggtgaaaat ggaatattgt
     4801 aatttggcat tgatttgctc cttttatctg tactcattct cttatgacta ctttatttta
     4861 gtaccttaaa cacggtttga aacatgattt tgatgtcccc aaagaaacta aactctcgga
     4921 gataggcgag gttatagcgc atcttttcag gaagaacatg ctccacatag gcctgatcaa
     4981 ctgacagacc tttctctgtc atttgactga tgatggtgtc ctcatccttg tagttgatgc
     5041 tggctggaga ggtaattcct gcttgcaaga gcaaggttgc catcatttca gtgctatact
     5101 gctctgtata acgtggcact tcaggtcgtg taccgacaaa ggacatctca cctttaagga
     5161 cattgaccaa ctgaggcagt tcgtccaaac ggacacgtcg gatgaaattt ccaaccttgg
     5221 taatacggct atcgttagca gaagtcacca gatttccttt tttatccgca tccgtcacca
     5281 tggtacggaa cttccaaatc ttgaaccgac ggttgtactg ggtcacacgc tcttgcttgt
     5341 aaatcactgg ccccttgcta tccaacttga tccaaatgct caagatgaga aagatgggag
     5401 aggtcagaac cagtaagacc aaggccaaaa cccagtccaa gcaacgcttg aaaatcagcg
     5461 aacccttcct tttagagaca agctggtagt aagactcaac ctcgcttgat ttcatttcca
     5521 caggcaagtc ttcccatttc agcatgctgt tctcctttgt ttttattata ctatttgtca
     5581 acactttttg tcaacacttt tcattatacc acaaaatgga aaaggcggtg aaaaaaatct
     5641 gtaatttgaa ggatttcctt ctagtactca atgaaaatca aagtacagct ttgagattgc
     5701 agataaagct gacatggttt aaagagattt tcaaagagta taggccatgg cccctgcctg
     5761 caaactatac atcttgtgat aggttcctcc cagagccaag agttcctcat gggttccact
     5821 ctcgataatg cgtcccttat ccaagacata gatgcagttg gcgtcttgaa tagtagaaag
     5881 gcggtgagct atagcaatag ttgttcggcc ctgtctcatc ttcgccagag aagcttgaac
     5941 caagctttct gtttcagagt caatattggc tgtcgcttca tccaaaatca ggattttagg
     6001 ctggctggcg actgttctag caaaggcaag aagctggcgt tgcccagtag agaagctcga
     6061 accacgctcg gaaacagggg agtcgtaccc ctgaggaagt tcttgaataa aggaatctgc
     6121 atccacaaag gctgccgcag cctgaacctg ctcatcactg atttcttggt acatggcgat
     6181 attggactta atagttccat gatagaggaa gggttcctgc aagaccaaac cgatgttttt
     6241 tctcagctct tcttgactga aatccctgat atccacatca tccaagagaa ctctccctga
     6301 ctggaattca taaaagcgca tgaggacatt gataatagac gatttccctg aacctgtatg
     6361 acctacaaag gcaatggttt cacccttatt aacagagaaa gaaatgtcat ccagaatcgg
     6421 atgtttaccg tcatatgaga aacacacatg ttcaaagcgg atattgcctt cttggacttt
     6481 ggcttgccca ttttcttgaa gaggttcata ggtcctctcg tctaccaggg caaagacacg
     6541 acctgcagaa accatagacg tttgcagtgt tgaaaagttt tgcgtcacct caatcaaagg
     6601 gtcaaagagg cggttgatgt actggataaa agcatacatg gtcccggccg ttatcccgat
     6661 agaaagacca cgatagccaa aataggccat caagacagca taacctagaa gtttcaacaa
     6721 actcatggca ggtctcaaaa agagggcatc caaggctaca gaacggttgg catagaccaa
     6781 gtgctcttgg ttgatttcat caaattctgc ctgcaggcgc ttctcttgat taaaggcctg
     6841 gattattctg attccctcga tgttttcagc cagcttgcta ttgatatctg acaagagact
     6901 tctggttttc tcaatgattt tcactgattt tttccgatag agattgacca aaaggaaaat
     6961 caaggggaga aagagcaaga ctaatgctgt caaacgaaaa tccaatacca acatggtata
     7021 aagggttgtc agaaagatga aaactgctga gataaagcta gataaaatcc cagaaaacat
     7081 atcactaatc gtctcggtat cgttggtcaa acgagaaacg atagaacctg ctggcgtctt
     7141 gtcaaagtaa gacatgccca gtttctccat attggcaaag gcatcccgac gaatatccct
     7201 aacaatactg taagacacgc gcgcaaagag aagattgccg acatactgaa ctacagtttg
     7261 taggatgtag agaccatagt agaccagcaa aacggtaacg gctagttggt taagattgct
     7321 gagatactgg tcgataaagt gggaagccac gaggggtatg acacttttaa tgaccgtcgt
     7381 cgctaggaga aaactgagtg ccaaaaaggt caggagtcta taaggcttga gataagacat
     7441 caagcgcttc aatacagccc attgttcttg tttattctgc atcttcttct cctttcattt
     7501 ctaactgctg agactggtag gtttgggcat accagccatc catcgctagc aagtcttcgt
     7561 gcctgcctcg ttcgataatt tgaccatttt gtagaactaa aataaaatct gcatggacaa
     7621 cagcactgag gcgatgggca gttataatgg ttgtcttgtc ctttcgcgtc tccttgagat
     7681 tgtcgataat cgcatactct gtcttggcat ctacggcgga taaggaatca tccaaaatca
     7741 agatatcagg gtttaaaatc atagcccgac tcatagccaa ccgttgcttt tgaccaccag
     7801 aaagactgac tcctttttca ccaatcagcg tatcaaatcc ttgaggcatg tctacaatat
     7861 cttggtaaac tcgggctagc ttagtagctt cctcaacctc tgaaaggggc aagttaggat
     7921 tgccaaagcg gatattgtct aggattgaag tcgcaaagag aaactggtcc tgaggaacat
     7981 agcccatgag actgcgaagt tctgtcaaac gatagtcccg aatatcgtaa ccgtttagat
     8041 aaatggcacc cttatccaca tcgtattcac gcaagaggag cttgattaag gacgttttcc
     8101 cagagcctgt ctgcccaacc aagcccagtg tttgcccttt tgccaaacta aagtgaatat
     8161 ccgtcagtgt ttcctcattt tcaaaagcaa agctgtcaat ggcatactcc aaacgcccat
     8221 tttcaatacc atccagagga aactcagggt cttgtacagg agattcctga gacaaaagat
     8281 tttcaatccg ctggtaagaa accttccctc gctgagtagt attaaagagg aagccgatgg
     8341 ccataagagg ccagaccagc atatccaaat agctgataaa ggtgactaga ttaccaactg
     8401 taatctgccc ttcctgaacc atcaaggagc caaccaaaag cgttaaaaca taggacgaac
     8461 caacaaacaa gagaaccata gggtcaaaga gactatcata tttcatggtt tgcaggttct
     8521 tttggaaggt taattcattg actgcctgaa aagacttcaa ctcgtctgcc tgataaccga
     8581 aagacttggt cactttgata cctgatacgg actcctgtac cttgttattg agttcagaaa
     8641 aagcagcttg ggattcgcca aaggccttat gagtctttct ccctaggcga ctagtcgcat
     8701 aggccatgaa aggtaggggg agaatggcaa caagagtcat ctgccatgag atgctaaaga
     8761 gcatggtcaa caaagtcacc agagccgtga tagaggcatc caccgcagac atgacaccgc
     8821 cacctgctaa acgagtcaag gcattgatat cattggttgc gtgtgccatc agatcacccg
     8881 tccgataggt ttgataaaag gctggcgaca tttttgtgaa atgcttaaac aagcgagacc
     8941 gcatgatctg tcccaagcaa taagaggtcc caaggatata catacgccac acatagcgca
     9001 aatagtacat accaaaggct gcaagtagca agtaaaatag gctaagaagg aggtcctgct
     9061 gggttaattg ccccgatgtg atggcatcaa tgactcgccc cataaccata ggaggaatga
     9121 gattgagaac ggaaaccaag accagggcca caatcccgac tagataacgg tgtttttcta
     9181 acttgaaaaa ccaccagagt ttttgaataa tggacataaa atccctttct gattgcaaat
     9241 ggaaacctga ggccaatact caatgaaaat caaagagcaa actaggaaac tagccgcagg
     9301 ctgctcaaag cactgctttg aggttgtaga tagaactgac gaagtcagta acctacatac
     9361 ggcaaggcga cgttgacgcg gtttgaagag attttcgaag agtacaagac cccaggtttt
     9421 tcttattcat aagttacgac tgtaacagca cccttgtcat attcagcaat aaagatattg
     9481 gctacattgt catgcccttg tttgctgagg ttatcaagca accactcttc gctacgacca
     9541 atcgattcca agacgtctac ttgaatcaca ccgtcagtca caactggata cttaggattt
     9601 tcatctccca tttgcacaac gatgagttgc ccattttgct cttgcacagc tcgtttgact
     9661 tgcttcatct ggaaaatccc ttggctacga agtttgaggg ctacatccga tgcagacaaa
     9721 ccaactgaac gacaggctta tgggtcaata ttcccatttt tgataaggag agttggctta
     9781 ccatcaatca aacgtttcac aaagtgaaca ttgttgttga gctatttgag agtcaagatc
     9841 agtttgatta aaatttcgat ataattaagt gtcatttgtt cacatccacc agttcaattg
     9901 cgtctgtctt atacaattcg actttctcta acaggtactt gtctggctca cttccgttta
     9961 gggcacgata atagcgatct cccaccttga taagtgcgcc atctgtgttt gtggaagtat
    10021 taacatggac ttctgacttg tctactccca aatctttgga aacaacctcg atgaaatgaa
    10081 gtgaagtttg aaatttattg ttagaaactt gattattttg ataagtcgtg atgctgacca
    10141 aaagcatagc taaataaggc cagagctgaa atcatgacca actcacgaaa tttggttcct
    10201 tttttatcat ggtatgcctt gaaagcaaaa aaaactgtca cagctaatag aacaatccca
    10261 aagccaacca tgataccatt ttgctgaccg atttggctga gtacatagtc ataagagtag
    10321 aatttcatgt attttcactc cctttcataa aatcattctt aattataaac gaaagagagt
    10381 aatttttcaa accatacgtt ggggaaatag atcttttttt ggtataataa aatctataat
    10441 ctgaatgaaa aaggtaactt tatgaaactt atctcatgga atattgattc cctaaacgct
    10501 gccctaacta gtgactcagc tcgtgccaaa ttgtcccaag aagtcctaca aaccttggtc
    10561 gctgaaaatg ctgatatcat tgctatccaa gaaaccaagc tttctgccaa aggacctaca
    10621 aagaaacacg tggaaatttt agaagaactc ttcccaggct acgaaaacac gtggcgctct
    10681 tcccaagagc ctgcccgtaa aggctatgct ggaaccatgt tcctttataa gaaagaactt
    10741 acacccacta tcagcttccc agaaatcggt gccccttcta ccatggactt ggaaggccgt
    10801 atcatcactc tagaatttga tgcatttttc gtaacccaag tttacactcc aaacgctggt
    10861 gacggtctca aacgcttgga agaacgccaa gtctgggatg tcaaatatgc ggagtatttg
    10921 gctgaactag acaaagaaaa accagtcctt gcgaccggtg actacaacgt agcccacaat
    10981 gaaatcgacc ttgcaaatcc tgctagcaac cgccgttcac ctggatttac tgacgaggaa
    11041 cgtgctggat ttaccaacct attggcaact ggatttactg ataccttccg ccatgttcat
    11101 ggcgatgttc ctgaacgcta cacttggtgg gcacaacgca gcaaaacttc taaaatcaac
    11161 aatacaggct ggagaatcga ctactggctc acaagtaacc gtatcgctga caaggtcact
    11221 aaatctgata tgattgactc aggtgctcgc caagaccata caccgattgt cttggaaatt
    11281 gacttgtaag gattttctat atggactaca atgcggtcat tcccgagttt cttgtatcga
    11341 atatcgaaca gtcccggtcc ttctactgtg gtttactggg ttttaggatt gaataccaac
    11401 gttcagaaga aaactttctc tttctcttga agagtgtcaa ctaatgttag aggagggaac
    11461 aaaggatcaa ctagctgaac tgacctaccc ctttggtcgt ggagtcaatc tctcctttgg
    11521 cataaaggat gttccaaaac tctatcaaaa agtgatggag gcaaactatc ctatttatcg
    11581 tcccttgacc aaaagaaagt ttcgtgtcag tgatccctat atctatcctc ataaatttgc
    11641 agttttggat ccagatggct attttttaag atttagcgag tagaataata aaaattggga
    11701 aaagtcttta aaagttagaa aaaggcatat tgtcaggtgt ctaaaaactt gatagtatgc
    11761 cttttatgat ggaaaaatct tctaatactc ttcgaaaatc tcttcaagcc acgtcaacgt
    11821 cgccttaccg taggtatata atactgactc tgtcaattct atccacaacc tcaaaacagt
    11881 gttttgaggt tgtggatagc ttcctagttt gctctttgat tttcatttag tataaatgtt
    11941 agcttacaat ggagtttttg ttcaccctct cttctgtttg ttcgtttttt aaaccaaaac
    12001 actaacaaaa tagttgattt ctaaagttat ttagtgtaaa ataaaaacat tggctcaagc
    12061 ctatttaaaa ttgaatatcg ttttacttgt ttatgtcgtg aattggcacg acgtttctac
    12121 aaggtgccgg aacacctaac aataagtaag tcagcagtga gatatggccg tttttgccat
    12181 gcctatttgg gggacttact tagagattaa gtaggtcttt tttgaatatt tttaatattt
    12241 cccttaatta gtgcggacgg gaggtgaaat tatcctgtga ataatttcag gaatccaggg
    12301 aatttcatcc ctaatttttg agcccctagc tctttcattt ctaggcttag gctaaaatag
    12361 ttccccgaac tattttactc tcaaaaatcc cgtcatagac aataacaaga ttattgtcta
    12421 tgataccact acaacgggaa atatttgata agactcgctt cgctcgtaat agttttatca
    12481 aatatatagc attagcaaaa gggatcacta tagaaacatt taaacaacaa gtaaattaac
    12541 ttttagactt taggaggtct tatgaaatta ttagaagagc gcatcctcaa ggatgggcat
    12601 atcttgggtg ataacatcct caaggtagat tcctttttaa cccaccaagt tgactttagc
    12661 ttgatgcgag agattggtaa ggtttttgcg gaaaaatttg ctgctgccgg cattaccaag
    12721 gtcgtaacca ttgaagcgtc gggtattgcc ccagccgttt ttacagctga agccttaaac
    12781 gttcccatga ttttcgccaa aaaagctaag aacatcacca tgaacgaagg catcttaact
    12841 gctcaagtct actcctttac caagcaggtg accagcaccg tttctatcgc tggaaaattc
    12901 ctctcaccag aggacaaggt tttgattatc gacgatttcc ttgctaatgg ccaagctgct
    12961 aaaggcttga ttcaaatcat cgaacaggcc ggtgccacag tccaagctat cggtatcgtg
    13021 attgagaaat ccttccaaga tggccgtgat ttgcttgaaa aagcaggcta ccctgtccta
    13081 tcacttgctc gtttggatcg ttttgaaaat ggtcaggtcg tatttaagga ggcagatctc
    13141 taatgcaaac tcaagaaaaa cactcgcaag cagccgttct tggcttgcag cacttactag
    13201 ccatgtactc aggatctatc ctggttccca tcatgattgc gacagccctt ggctattcag
    13261 ctgagcagtt gacctacctg atttctacag atatcttcat gtgtggggtg gcaaccttcc
    13321 tccaactcca actcaacaaa tactttggga ttggactccc agtcgttctt ggagttgcat
    13381 tccagtcggt cgctcccttg attatgattg ggcaaagcca tggtagtggc gctatgtttg
    13441 gtgcccttat cgcatctggg atttacgtgg ttcttgtttc aggcatcttc tcaaaagtgg
    13501 ccaatctctt cccctctatc gtaacaggat ctgttattac cacgattggt ttaaccttga
    13561 tccctgtcgc tattggaaat atgggaaata acgttccaga gccaactggt caaagtctct
    13621 tgctttcagc tattactgtt ctgattatcc tcttgatcaa catctttacc aaaggattta
    13681 tcaagtctat ctctattttg attggtctgg ttgttggaac tgccattgct gctactatgg
    13741 gcttggtgga cttctctcct gttgcggtag ctccacttgt ccatgtccca actccactct
    13801 actttgggat gccaaccttt gaaatctcat ctattgtcat gatgtgtatc atcgcaacgg
    13861 tgtctatggt tgagtcaact ggtgtttatc tagccttgtc tgatatcaca aaagatccaa
    13921 tcgacagcac gcgccttcgc aacggttacc gcgcagaagg tttggccgta cttctcggag
    13981 gaatctttaa caccttccct tacaccggat tttcacaaaa cgttggtttg gttaaattgt
    14041 caggcatcaa aaaacgcctg ccaatctact acgcagctgg tttcctggtt ctccttggac
    14101 tgcttcctaa gtttggtgcc cttgcccaaa tcattccaag ctccgtcctc ggcggtgcca
    14161 tgctggtgat gtttggtttt gtatctattc aagggatgca aatcctagcc cgagttgact
    14221 ttgctaacaa tgaacacaac ttccttatcg cagctgtttc aatcgctgca ggtgtcggtc
    14281 tcaacaacag taatctcttt gtcagcatgc cgacagcctt ccaaatgttc ttctcaaacg
    14341 gaatcgtcgt agccagccta ctcgccattg tcctcaatgc cgtattaaat cataaaaaga
    14401 aataagaaaa agaggtgcga gcctcttttt tgtttatcac aaattgatta taaatacaat
    14461 caatcccaat tcccatctaa ttgaattgga cctatttcaa attctttaaa atattatgtt
    14521 ccaaatcgtt aatattatat aagaatggta aaatatcaaa tgtttctcgt aaattattct
    14581 tagccttata ccaaccttgt ccatcagtaa tccacataaa ctcaacatta ggaatagctt
    14641 taatttcttc agcaatcatt ttgtaagatc tagccgtctc atttaatttt gaaccacttc
    14701 ctgaataaaa attaacctca atcagataaa gcacttgttc attttttatg acaaaatcaa
    14761 aacgtttcac agtattccca tcattcgtaa tagcagataa atcgacagaa aaaatttcct
    14821 ctatttcatt ttgttcaatc tctttaaata aattctctcc taaaatatac ccttcagctt
    14881 caagataact ttgaacaata ttttccatcg catctccagt ccgatttttg cgcccattag
    14941 tatccatacc aacctcaaca cctgtaacgt agtcaactaa atttgatact aaatgatttt
    15001 gcaataaatc aaatatacct gatttttcca aaaacatagt atattcttca atactatagt
    15061 ttcgtttact aaaatcaaaa tagaaatctt ttatcggatc cttaacaata atcgtatctc
    15121 tcaaacgttt agcaataagt agaggcacaa ctttcaaaat ttctggataa ttttgatata
    15181 agtctaaaaa atcctcttgt atatttttac taccaataag actattcata atatttagag
    15241 caacttttat actagataca tttttataaa ccttgggaaa atctgtataa taagtccagt
    15301 cagctactgt gtcggtcatc gttgacaacc actcatcaaa atttctagtt tgtttcattt
    15361 tctgcctcca acctcttcct tgcaattttt aaatattctt tttcagcatc gatacctata
    15421 aatcttctac ctaaccgctt cgcaacaaca cccgtagtgc cactaccaac aaatgggtct
    15481 agaatatagt caccctcttt agtagaggct aaaataatgc gttctaacaa atactctggt
    15541 ttttgagttg gatgtttccc agcccatttt tcaactttct ttgttaaaga acccgtccag
    15601 acatctttca tttgttttcc atcattcaat tctttcatta aatcataatt gtagtaatga
    15661 cgagcttttt tatcattttt tctggcccat aaaatggttt cagtagaatg ggtaaaataa
    15721 cgacaagata aattgggggc agggtttgtt ttctgccaag taatattatt cagaatttta
    15781 aaaccttctt gttctaatgc cattccaact gagtatatgt tgtgcaaact acctgaaatc
    15841 cataccgtcc cattaggctt cagaacttct tttgctaggc gaatccattt acgattaaac
    15901 tcatgttttt cttcgaatga agaaatttta tcccaatctc ctttatcaac agaaacaact
    15961 tgtcccccag aattagatat tccaccatta cttaaaaaat aaggtggatc agcaaaaatc
    16021 atatccatac tttctggttt catttttgat aagaacttga acgtatctga atgaacaaga
    16081 atcatcttat ttttattgta gtatggtttt gtcataagaa cacctccata cttatattcg
    16141 ttatttttca taatttgtga ctataatttc agaaattttt cctcgacttg aagattttgc
    16201 tccattagtt cgggtagctt caacataatg tatattaaaa tccttataca actcctctac
    16261 taaagcacta gaagaatttg ataacataac ataagctcct gtatcactca atctcttaaa
    16321 ggcatctctt aatcttactt gatctgcaaa agagaatccc tcatgagtat aagacgtaaa
    16381 tgcactcgtc tcagacaatg gaatatatgg agggtcaaaa tacacaaaat ctcctgttcg
    16441 aacatctaca attgcctttt caaaatctcc cactttaatt tctagttgat tgttatttag
    16501 ataaactgaa atagcagata tcaattcctc atcaacaatt ttaggattct tataacgtcc
    16561 atatggaaca ttaaattggt tcttagaatt cacacgatat agaccattaa agttcactct
    16621 caacatatat agaatacgtg cagctctttg tacttcggac atcatatcta ttctttcatc
    16681 acgatctgca gaacgtaaat ctaaataata ttcttttgaa ttatattcct gatgaacttt
    16741 caaaatttca atcaattctt gaggattgtc cttaatttgt tgatagcaat ttattagttc
    16801 agcgttaaaa tcattaataa ctgcatcttt aggagccaaa tcaaaaaata aagctccacc
    16861 tccaacaaaa ggttcgaaat acctgttata ggttttaggc attaattctc taataacagg
    16921 caataattgt cttttaccac ctgtccattt cgtgaacggt tgtaaagtaa ctttctttat
    16981 ttcttttatc ttcatgcttt taattatacc atagaaatta aaacacaact cttgtatttt
    17041 ctaccacatt actaaatttt ctattttaga attaaatcat atcaatataa aaaaatgagt
    17101 aaggcaaatc ttataaattc caaaattcac ctcactctat taaattactc tttatatcct
    17161 tacccgtctt tcctcagacc tttggcctgt gacaaacata gtaaaggttg ctttgcagac
    17221 atttctgccc tcttgattgg tgatatccac atccatcaca caggttgtac gaccttgatg
    17281 gacacattct cctttaatgg tcaatacatc gtcgagtttt cctgccttaa ggtagttgat
    17341 agaggattgg agtgtcaccc catcaagtcc cagcgagata accaccaaac cactgatttg
    17401 gtcacatagt gtgaatagat agccaccatg ggcattgcca tagtagttga gcgacgagtt
    17461 cactactttg gtcgtcacca caacgtgacc atctctcatt ttttcaattt cgtaattttc
    17521 aaaggcagat atagcgtcaa aatgaaagtc tttcatcgtt tcctcctgat atttcaaatg
    17581 acactatgat actacttttt ataggactgt gcaaggagaa agctcctagt ctagtaaaat
    17641 tccgacatgt tccacaaagc gcataaaggc tgtctgccct tgttctgtct gcttgtagac
    17701 tcctgcatcc tcaagtacac gcgcaaagat agcacccaca gagtccttga cgattgcaag
    17761 ggctttttct ttatccgtta ggtctggatg ttgggatttg agttggtctg cccactcctg
    17821 atgataatcg gcaactgtaa cagcttctcc tacaagatag ctagcgactt gctctacttc
    17881 ttctttcaga cgtggtggca agattgccaa gcccatgacc tcaatcaagc cgatattttc
    17941 cttcttgata tgttggacat ccttgtgggg atgatagatg ccatcaggat gctctgctga
    18001 agtctgattg tctcgcaaga ccaagtccaa ctcaaactgt ccatcacgct tacgcgctat
    18061 tggtgtgatg gtgtgatgcg gtgtcccgtc tgtctctgct aaaatctgca cactagaatc
    18121 tgaatactga cgccattcct ggaaaatctt atcagctaag ttaatcaaat cctctttgga
    18181 atccgaagtc aagcgtagga cagacattgg ccacttgata attccagcct tgacctgctc
    18241 aaaaccagca aatcggaagg tcttttgcaa gggagccaat tccataggaa atacgtgacg
    18301 gcctccctga taatgatcat gagttagaat agagcccccc acaatcggca ggtcggcatt
    18361 agatccagca aaatatcctg gaaactgctc tacgatagcc aacagacgtt caaaactctg
    18421 acgactaatg gccatgggac gatgctggcc atctaaaaag atacaatgct cattaaagta
    18481 agcatagggc gaatactgga aaccccattc ctgaccaacc atttcaaaac ggataatccg
    18541 gtggttgctg cgagctgggt ggttgacccg accatggtaa ccctcattct ctaaacaaag
    18601 ttgacactga ggataattac tagcttgcac caacttggct accgcaatct ctttcggatc
    18661 cttttcaggc ttagagagat tgatggtaat ttcaagttct ccataatcag atggaacacg
    18721 ataagcgata tttttagcaa tggccttgag tttgatgtag tcatttttct gactgagttg
    18781 ataaaaatcc tctatcgctt gctcaggaga gtaggcatag gcttcccaga agtcacgatt
    18841 gacctgactc ggacaaggag tcaccaagtc catcaattca gtaccaagga tttcacgcgc
    18901 agtctgacta tcctcaatcg tctctaatcg aacggcttcc tcaactagct ggtccttgag
    18961 gtcaatcaat ttatccagat tggtctcaac ttccaaaaca ccttctccca ctcgtgccaa
    19021 aacacgattg gtcaggtaga ttcgatccat ttcctcaaat gaactttcag aaatgacatg
    19081 tgttacaaat ttatctacta aggtcactat agagcctcct tttgactagt caaggacgcg
    19141 agtgccacct gcaacttcag cgatatagaa gcttggagcg tatccaacta cttcctcgta
    19201 gtgtttgcct acagcttcct taaaggcctc aacagtatct ttttgaacca aggcaatggc
    19261 acagccacca aaaccagccc ctgtcatacg agcaccgaga actccttctt gtgcccaagc
    19321 tgtgtgaaca agagtatcca attccaaacc agttacttca taatcatgct ccagagaaac
    19381 gtgtgacgca ttcatcaaac gaccaaatgt ttccaaatca cctgcttgaa gggctgcttg
    19441 agctttaagg gtacgttggt tttcaaacac agcatggcga gcacgtttca aacgattttc
    19501 atctttaatc agatagctat attggtcaac ggcccactcg tccaattcac ccagggtctg
    19561 aatatccaag gcaacttgca attcttccac tgctttttca cactcagcac gacgttcatt
    19621 gtatttagag tccgccaatt cacggcgttt gttggtgttc atgataacaa cgacattgtc
    19681 cttcaaatca agtggcacca agtcgtattc taaagtattg gtatctaagt aaatagcacg
    19741 ttggtcagcc cccataccaa tagcaaattg gtccatgata ccagagttga ctccgataaa
    19801 gttattttct gtttgttttc cgattttaac caaatcgaga cgctctagtt ttaaatcaaa
    19861 gagatgctct gccacgactc ctgtcaagag ctccaaggat gctgaagaag acaaaccagc
    19921 accatttggg atattcccat aaacataaaa atcaaaacct ttgtcaatca cgtgcccagc
    19981 ttcttgcaag aaatgaagga caccttttgg ataattggtc cagttgtgct ctttttcaaa
    20041 cttgaggtca gcgagaggca cttcgataat gcccttgtcc tcaaagttag ctgagtagaa
    20101 acgcaagact tggtcgtcac gcttacgagc tgcaccgtaa gttcccaagg aaatagcagc
    20161 aggaaaaacg tgcccaccgt tgtagtctgt gtgttcacca atcaaattaa tgcggcctgg
    20221 tgaaaagaag gtttggtctg cttcttgacc aaaaacagca agaaagtctt tgcgaagggc
    20281 ttcagtagta agatgttgtg ccatatgaat tctcctttga ctgtccgtta agccacctta
    20341 acgtataatc gttttctttc tattgtatcc aaggaatttt ccaagatcaa tcctttttac
    20401 taaaatttta gtaaaatatc agtaaaaaga agaaaaacat gatatactaa cctaatcaag
    20461 gaggatttat ggctacctta aaagacattg cacagctagc ctctgtctct atcgcgaccg
    20521 tatcccgcgt cctcaatcgc gaccagagcc tatctgttac agaagaaacc agacaccgta
    20581 ttttaaccgt tgctgaagag ctgggctaca ccaagcacct caagacaggc gagtcccaca
    20641 aacccaagca aaagattgcc attatccaat gggtcagcga acaaggggag ctggacgacc
    20701 tctactacta ccagattcgc ctaggaatag aaaaaagagc ccaagagttg gactatgata
    20761 tcttgcgcta ttttaatgac catcctttta ccttaagcga ggaagtgatt gggattctct
    20821 gcattggaaa atttagccga gctcagattt ctgcctttga agaataccaa aaacccctgg
    20881 tctttctaga cagtgataca ctttctctgg ggcatacttg tattattacg gatttttaca
    20941 ctgccatgaa acaggttgtc gattatttcc tcagccaagg aatggaccgt atcgggattc
    21001 taacaggcct tgaagaaaca actgaccaag aagaaatcat tcaggataag cggctggaaa
    21061 atttcagaaa ctacagtcaa gcaaaaggaa tctatcatga tgaactggtc tttcaaggaa
    21121 actttactgc ccagtctggc tatgacttga tgaaggaagc cattcagaac ttgggagacc
    21181 aactcccacc agcatttttc gcagccagcg atagtttagc tatcggtgcc cttcgtgccc
    21241 tccaagaagc tggaatcagc ctaccagacc gtgtcagcct catttccttt aacgacacca
    21301 gcctgaccaa gcaggtttat cctcccctct ccagcatcac agtttatact gaagaaatgg
    21361 ggcgagcagg tatggatatt cttaacaagg aagtcctcca cgatcgcaaa attcctagcc
    21421 tgaccatgct gggaaccaga ctgacattaa gagaaagtac cctaaatcaa gaataggata
    21481 acataaaaaa cgaatagagt tctaaaactc ctattcgttt tttattcgat tacaatcata
    21541 gacttaatgg tcttacgttc atccatatct ttgtaggctt ggtcgatatc ttccagttta
    21601 taacttgaag taaagacgcg acctggattg atatcaccat caaggacggc ttttagtaaa
    21661 aattgcttat cgtatgttgt agcagaagct gccccacctg ctacagagat attttgcata
    21721 aatgtcgaac caagagcacg attattatag tgtgggactc ctacaaagcc catacgccct
    21781 ccattatgaa gaacacctag cgcctgttct atagcagcct ccgtaccaac acattcaagt
    21841 gctgcgtctg ctcctccacc gaggatttca cgcaccttgg taattccttc ttgaccacgt
    21901 tctgcaacaa caactgtcgc acctgactcc atagccatct tttgacggtc ttcatgacgg
    21961 ctcataagga taatttgtga tgctccacgc atcttagccg cgatgacagc acattgacca
    22021 acagccccat caccgataac aacaaccttg tccccttttt gaacatttgc aacacgcgcc
    22081 gcatgatagc ctgtcggcat gacatctgca agagtcaaaa gggacttgag catcccttct
    22141 gtatagtcag aaggttgacc agggatttta accagcgccc agtttgcata gtggaagcga
    22201 atatattctg cctgaaaatc accccccaaa ttattgccaa tatgattgtc gcaagaaccg
    22261 tcaaatccag caagacaggc atcacactca ccacatccat gtgtaaaagg gacaatcaca
    22321 aaatcacctg gtttcaccgt cgtaatggct tccccagctt cttcaacaat cccaatcgct
    22381 tcgtgtccac tatttttgtg tccagctttc gtttctggat tacggtacct ccataaatct
    22441 gaaccacaaa cgcacgcacg aaccacacga ataatcacat catccacttc tattatttgc
    22501 ggacgttcaa tgctagcaag tccaacctga cctgcctttg tatatactgc tgatttcatt
    22561 taaaattttc cttccttata aagtttaatt ttgagattta aacgatttaa agctgtctgt
    22621 agctgagaca agcgctcctc taatttttgc ttttcctctt ctaaaatacc aagcctctcc
    22681 tctctcgttt catctccctt ttggtagagc gacatatagt caactaaact atctacagat
    22741 acacccgccg aacgaaaaca cttaataaat tccagcgctt cgatatcctg atcttgaaaa
    22801 tcacgaatcc cagtagcagt acgagtaatc ggtggcacaa gaccaacccg ttcataatac
    22861 cgaatcgtat ccgctgaaat tcccaacaaa tcactggcag atttaatatt cacacgctaa
    22921 cctcctctca tttcattcac tgaattcacg ctattctaac tcacttatct tattatataa
    22981 ctttgagttg actccaagtc aagcattttg tatctaatcg aaaaattttt atcattaaca
    23041 atcatatatc cggagtttat acaatgctct tctcttacca atttttgaaa tatttaaata
    23101 ataaagaaat tactctattc caaataagat agtacccaag tatttttcac ttttttctaa
    23161 tgttgatgct cataactccg ttccaagtca cacacctttc gcttatgggt ttggtgagtt
    23221 tctaggtcag catcaatttc aatggtaata ttttgaaaac cacaatcttt taggaaaatt
    23281 cgaatagact ctttacaagt ttccatatgt tccatttctt ttagacaaac atggacaatg
    23341 gcattttttt ccaaagcatc catagtccag agattaagct gattaaggct ggccacattg
    23401 tccaatcgct ccaggccact ctttacttgc ttgatatcaa gaccttctgg cacagcatcc
    23461 aagaaaatct tgagtgtaga ccaaaaacgt ggaagggctt ttgaaagaat aaagaaagaa
    23521 atgacaaggg acaaaagagg atctaggata taccagtccg taaatcgaag aacaatcgcc
    23581 atcaggataa cagctaccca ccctagcgta tcttccagaa aatgcagact cagaatagac
    23641 tcattctttg tctttccctt accaaccacc agactcgcta acagattgat agtaatcgca
    23701 ataattccta accagagaat cccctcatca ttgactggtt gcggatgcaa aatcttcgtg
    23761 acattttcca aaatgactag aacagagccc gttacgagaa tcacagctgt taccaaggct
    23821 cctagcaggc taaaccgctt atagcccaag gtgtactgat tgtcttcttc acgattggag
    23881 attgtttcta gaaaagctga tattccaatt gcaatcgcat ctcccaagtc atgcacagag
    23941 tcagcaagaa cagcgctaga accaaatact ccacctgcaa taaactcaac aatggcataa
    24001 gtcaaattta agaaaaaagc cacccaaaca gcatattttg ccttcatatt tctcattcct
    24061 ttgttataat agatttatga acaccttgtt cattatcatt atccactaaa actcaaagaa
    24121 aggatagaag caaaacgtca gctttattca gttctgaaca atttgcctca agtgtccata
    24181 tgactaacat tgaccgccgt atcagcaaaa ccaaaaaagc catctatcaa gcttttatac
    24241 aacttttgaa tgctaagggc tacgaggcca ctactgttca ggatatcatt gatctcgcag
    24301 atgtgggacg atccaccttt tactgtcact atgagagtaa ggagcttctt ctggaccagc
    24361 tctgccgcta cctcttccat catctttttg aaagagagca agccatttca accgaagatt
    24421 acctagccca cctctttctg cattttcaga aaaaccaaga ccatatcacc agtctactat
    24481 tctccaaaaa tgactatttc ctccgtcaac tacagagaga gcttgagcac cacgtctatc
    24541 ccatgctggc tgctgatttg aagcaaactc acccaagtct acctacttct tacctccaac
    24601 acttggtcgt gtccaacttt atcgagacat tgacctggtg gctcaaaaaa ggtcaagatt
    24661 tcacagacca ggaagttgtc caattttatc tagaccttct cattcctaaa aattgaatat
    24721 agagtaaagc ttcagttgtc ttatttctag gttactgagt tttttatctt ttcaacaaca
    24781 aaagaggacc cgccgatcct ctttttcata ctataaatcc ttgattatca attatatctg
    24841 ttttaatcga aatctcaaaa cagcactttc aaacatcttt tcctagttaa gtaaatcagt
    24901 attctgctta gctgccttgc tccattgata ccaaccaact agactgttaa tgagataaat
    24961 tagatatttc ccttgaattt gcaggctttc tccccaccag agatagattg aaaagacatt
    25021 ggtagccgcc cagaatatcc actgttcacg gtaaacagct gtcatgagga tttgccctac
    25081 cccattggtt gcatctgtga ttgaatcacg atagggacga ttggcaccaa tagactgata
    25141 aatgaagcca aaggccaacc accaaagcac actaatggaa agatactttg tccagccctt
    25201 gccgtccagt ttacgcgcga caaactcctg cttttccttc ttaaactgtg cctgataaat
    25261 ccaaactaga agtccaattg gctgcatgac tgtgaagtaa agtgtcgtca gcacctcacc
    25321 ataaaagcct ttctgtaggg ccaaaataag gtaaataaca gagttaatca agccaaaaag
    25381 ataattactt gctcgacctt ccgatacaaa gattacacag ataatccctg tcaagctaca
    25441 aatcatccca atccagtcaa caatacgatg ttcgtaaacc aactccagcc agagaggaaa
    25501 acttcctaaa accagcaaat aaatccactg ggcaaaacta cgatgggcaa agaggtcatc
    25561 ccagatagcc ttcatagttc ctgaaaatcc taaatcagcc atagccgcaa ccatacgacg
    25621 gtaaccacct gacatttcac ctagggttgt tttgatattt tcaattttct tttgcaaata
    25681 agtatgcatc atttctcctt ttgtttttaa agagccgtgt ctggatagac tttcggacgc
    25741 aacgctctat tagataatga actgcctata cacaagattt ctaaccttag tcgacatgag
    25801 ctgaaacctc ttatttgtta agtagttcac aaaatattat acacctattt tatgaatagt
    25861 caactgtctt tacagtaaaa ttttagaaaa tcatgaaaat tttctctttc tttccatttt
    25921 aagtgacatt cagtcattct cacatcaaaa aagcccagac gaaattgtct gagcattctt
    25981 ttatctagtc gtttaaggaa gttgagttca gtatgtttaa agtctctgtc ccatcatttc
    26041 ttcaacaaac cttgttcttg gagaaactcc ttggctactt gctttgctga cttgccttca
    26101 acaccgactt ggtagttgag ctggctcatc tggctttctg taatcttacc agccaatgta
    26161 ttaagaactc tttccaactc tgggtgtttc ttgagaagag cttctttcat gagtggagcc
    26221 ccttgataag gtgggaagag ttgcttgtca tcttccaaga cctgtaaatc ataacgctcc
    26281 aattccgcat cagtcgaata ggcatccgtg atttgaatat cccctgactg aattgcctga
    26341 taacgaagag ctggctgcat agtcgccaca ttgagattga gaccatacat tgattgcaag
    26401 cccttatttc catcttcacg gtcgttaaac tcaagcgtaa agcctgcctt cagctgccct
    26461 tccacttttt tcaaatccga aatggtcttc aagccatatt cttgagcaat ctttttcgga
    26521 acagctacag cataggtatt ttgataagac ataggtttga gataggctag atgatcctgc
    26581 ttggcaatgc catcacgcgc cacttgataa acctgctctg gctcatgact caccttgggt
    26641 gatggttgaa gcaaactttc agtcaccgta ccggtaaatt caggataaat gtcaatatca
    26701 ccttttttca gagcttcata aaggaaactt gtcgtcccaa aattcggttt aacagtcgca
    26761 gtcatgctgg tattttcttc aatcagcaat ttatacatat tagccaaaat ttctggttct
    26821 gggcctattt tcccagcaat aatcaagttt tccttctctt tttgagccaa aagggctgga
    26881 ctataagaca gacccagtaa taaagccacc aaggcaaaac cagagaaaat cgtccgcaat
    26941 ttcgcttttt ccattacttt tagtaggaag ttaaaggcaa tagctagcac tgcagaagaa
    27001 agggccccaa tcaaaatcaa actggcatta ttacggtcaa ttcccaacag gataaaggaa
    27061 cccagtcccc ctgccccaat caaggtcgcc aaggtagccg taccgataat caagaccgct
    27121 gccgtccgaa ttccagacat gataacaggc atggcaagtg gaatttcaaa cttcttgaga
    27181 cgctcccatc tggtcatccc aaaggcaatc ccagcctctt gaagactcgg atcaattccc
    27241 ttcagcccag taatagtatt ttgtaaaatc ggaaaaatcg cataaatcac tagagctgtc
    27301 aaagccggca aggtcccaat tcccatcaaa gggataaaga gccccaacaa ggccagagac
    27361 gggatggtct ggaaaatccc tgcaatctgt aagacccaat ccgccagctt ctcatgatag
    27421 cgaagaaaaa cagccaaggg aatcgcaagc aaaatagcta gtaacaaggt caaaagtgac
    27481 aactgcaaat gttgagatag agctgtcaac caatcactaa aacgatcctg aaaagttgca
    27541 attaaattag tcatgaacac tacctccaaa caagtctgct acaaagtctg ttgcaggcac
    27601 ttttaaaatt gtctcgggat tcgctacctg gcgaatttct ccatcctgca agacagcaat
    27661 acggtccgcc aacttcaagg cttcatccgt atcatgggtt acaaaaatcg ttgtcatccc
    27721 aaactcttta tgcaattctt ttgtcagaac ctgcaactgt tttctcgaaa tagcatccaa
    27781 ggccgaaaag ggttcatcca tgaggaaaat cttgggctga ccaatcatag ctcggacaat
    27841 accgacccgt tgctgttctc caccagataa ttcactaggt aagcgatgcc catactcggc
    27901 tactggtaaa ccaaccttag ccaaaagctc ttctgttttc ttcgtaattt cttccttgct
    27961 ccaccccttc atttcaggaa tgagagcaat attttccgca actgttagat ttggaaaaag
    28021 agcaatagcc tgtaaaacat aaccagtaga aagacgaagt tcacgctcat catagtcttt
    28081 gatgcgcttc ccatccatat aaatatttcc atcagttggt tccaaaagac ggttaatcat
    28141 cttgagcatg gtcgtcttac ctgacccaga aggccctact aaaaccataa attccccatc
    28201 ctcaatctgt aagttgacat ctctcaagac atccttttct gtgtagcgca gtgctacatt
    28261 tttgtattca atcattcttt gtcctcaatt taaaacttcc ctcgattagt caagtcttct
    28321 accttaggca tgacttcctt attatcccaa tgctccacaa ttttcccgtt ctctaaacgg
    28381 aagatatcgt actgggcata agcaacgcca tcaatctgag tctgaccata gctaaccaca
    28441 tagtttcctt gtcctaagag ttggaaaaca aagtcaaaag tgacactata ttcagccaca
    28501 tagtttttat aagcagcact tccttgtcca atctcatgat tatgctgaat caaatcgtct
    28561 gccacataat cactccactg ctctagctcc ccattttgga aaatttctgt caagaaacgg
    28621 cgaaccagct ttttattttc tgctttctta tccaaatcct tgatttcaaa atctccaaaa
    28681 atttgatcta gttggtcatt ttcaggtgtt cgatagtagt caatgacatc ccaatgctca
    28741 acaatacaac cattctcatc ctcacggaaa gtatccgtcg tcacccattg agcttctcca
    28801 ccattcagat attgatgaac atgaacaaag accagattgc catcctcaat ggtgcggaca
    28861 atcttaatct gacgctctgg atgacgctca aagaaatctg caaagaaggc tgcaaatcct
    28921 tctttcccgt caggaacacc tgtcgaatgt tggatatagg tatcccctac agactgggct
    28981 tgagcctcag caactcgccc gtcttgaatg gcatggatgt ataggttgtg agcatttttc
    29041 acttgttgtg acatattcta aacctcattt cccttctctt tcagattcgc caaaattctt
    29101 tcttgaaaac cttcaaattg gtgaatttct tcctctgaaa atcctttgta aaagatagta
    29161 tccaatttct gactgacacg atgccccact tctttctgga acttgcctaa ctccgttaaa
    29221 actaaatact tcttacgctt gtcttttcca cacggactaa caattacaag cttttgttcc
    29281 tctagctttt ttatcatagt cgtcagcgta ttattcgcaa gcccagtcgc aagcgcgata
    29341 tctgtcgcag ttgcgcagcc agtttcacta ttccataaaa ccgctaaaat cttgccctgt
    29401 tcacccctat aaagagcctc aggatcttga ctcagtaact tttgaaaaat ccgcccattc
    29461 aacaaacgaa tatgatgggc tagcaaatga ctatctttca taacacctcc aatttatttc
    29521 gatatcgaaa tgaataaaat aattgtaaca ctcatcgttc taactgtcaa ctatttcgat
    29581 ttggaaataa tttttgataa ttatccacac caccatactc cggctcaact aacttttaac
    29641 gagagtttct aaactccttc gtcctccagt ctacaaaagc cttccattcg tactatccta
    29701 tattttatga ggggacacat ttttcctatc agaccattta ttttaaagat agaagtaaat
    29761 cataattgct tccatctgtt cttttatagt atattgaagt tagactagag cactgtatct
    29821 tctaaaacat tgatagaaag cgatttgaat ttcccaatca atttgttcgt atttatagca
    29881 ttttgaaact agaataggac accatgactg ctaaaagatt tctataaatt catttgattt
    29941 tcctaatcaa tttgttcata tcttatttca ttccgctata atttcacctt accctatctt
    30001 tttcgtagca cccttcaaac agcctatccc ctaccgtttg acgattcctc acttcgctcc
    30061 acttccatta cagaagtttc ttcactacta tgggctcggc tgacttctca tgattccttg
    30121 ttactactat ttgaacgctc acgagataga tcttacaaaa aatgctttga tccacaatgg
    30181 aatcaaagca ttttaaagag ttcctcatac ataagcgcag aagtcgcagt tcctctgtac
    30241 ttggcttctt ctcttttgat aaagcgagcc aagttgagca actcaggtgc tggatgtttg
    30301 ggatttagga gcaattcacg attgaccagg cctgagagac gaactgcctg caattgctca
    30361 tttgtagtag gcagtttttt agcagtctct aggagagcag caactaaatc ttcactcaaa
    30421 tcatgtcgag catgattgta aagatctttt ataaggcttt ctaggtttgg ttctgccatc
    30481 cctaccacct cccttatggt ttaataatgt ttaatcaaat caaccgttga acgatccaat
    30541 ttcttcacca aggcttgtaa gaaagcttgc gcttctagga agtcatccat tgcatagagg
    30601 gtttggtgag aatggatata acgagcgcag acaccgatag ttgttgatgg gacaccacca
    30661 tttttcagat gagctgcacc tgcatctgtt ccgcctttac cacagtagta ttggtacttg
    30721 ataccagctt cttcagccgt tgtcaaaaga aaatccttca tccctgggag aagcaagtga
    30781 cctggatcat agaaacgaat caaggttcca tctccaatct tgccttgacc accgtagaca
    30841 tcacctgctg gtgagcaatc aactgcgagg aagacttctg ggtcaaactt ggttgtagag
    30901 gtatgagcgc cacgcagacc aacttcttct tggacgttag aacccagata gagttcattg
    30961 ccgagttttt gacctgataa agcttcagct agctcgctta ccatgaggac accgtagcgg
    31021 ttatcccaag cttttgagat gatatttttt tcattggctg tcaaaattgc agaactatct
    31081 ggtacgatgg tatcaccagg acggatgcca aaactttctg cctcagcctt gtccgcaaaa
    31141 ccaccatcaa aaacgatatc ggcaatggct ggcatggttg gtcccccctt tccacgagtc
    31201 aaatgcggag gaacagaacc tgaaatcaca ggaatttcat gaccatcacg agtcaagagt
    31261 ttgaaacgtt ggctgctaac caccatgggg ttccagccac cgatttctac gacacggaag
    31321 gtaccatctg gcttgatttc gctgaccata aaaccaactt cgtccatatg agaagcgacc
    31381 aagacgcgcg gtgcatccac agcttctgaa tgtttgatac caaaaatacc acccaagcca
    31441 tctgtcacca cttcatccac atgcggtgtc aacttttcac gaagataagc acggacaggc
    31501 gcttcatgac ctgagactgc agcaagttct gttacttctt taatttttga aaataatgtt
    31561 gtcatttcag ttccttcttt ctttcatcca ttttaccact ttttatagga gaaggatagt
    31621 gggaaggtgg atttctaagt tagtatctta gtcctgctct atcttagaaa aggatagtat
    31681 tctcttgcat gtagtgcaaa atctagtaaa cattccaaaa ttaactcgaa tatttatttc
    31741 caaacaaaaa aacaatacac catcaaagtt gtttgtattt ttcatgaaat ttacagaaaa
    31801 tagttgactt ccctttcttc tttctttaaa tatatagttg gttgagtttg gaatagtacg
    31861 ctgtagctgc tgaaacattt ctagaaatta atttgacttt cctaatagag ttgttcatat
    31921 cttatttcaa tttactatag tacaaaacta gaaaaggaaa aaatcatgac caaaacaatt
    31981 cttgttacag gtgggactag ctacattggc tcccataccg ttaaagctct tttaaatgct
    32041 ggctataagg tgcatgtcct cgataatctc tccacaggaa atcgagcggc tgtggatagt
    32101 cgtgctagct ttaaagaact ggatgtttat gatgctagcg ccttaaaggc ttacttagaa
    32161 gaaaatcaga ttgatgctgt cctccattgt gcaggtgaaa ttgttgtgag cgaaagtatt
    32221 gaaaatccaa gtaaatactt cactgccaac gttgctggta tgaaccaagt tctcaaagtc
    32281 ttatctgaag ttggcattca aaaaatcatg ttctcttcga ctgcttctct ctatggaaat
    32341 aactgtattg acaagccagt gactgaagat accctgctcg accctgtcaa cccttatgca
    32401 gagacaaaac tgatgggcga acgaatgatt tactggatgg ccaatcgcta cgactggaaa
    32461 tatgttattt tccgttactt taatgttgct ggggctgaaa tggatgcttc aaacggtctg
    32521 cgtgtgaaaa atccaactca catcattcca aatatcaaca agaccgcatt gggacaaaat
    32581 gatagcttaa aaatatttgg agatgactac gatacacgtg acggttcatg tattcgagat
    32641 tacatttatg tcttggacct tgcataggct catgttaaag gaatgaacta cctatttcaa
    32701 gaagacagtt cttctcaaat ctttaactta ggaactaaaa aaggctatac tatcaaagaa
    32761 atctttaaaa ctgctgaaga attactaaat taaaaaatcc cacacgagat tgttgctcga
    32821 cgtgctagtg acccagccag tgtcctagca gatgcttcaa aagctaaaca atatcttgat
    32881 tggacggcta gctactccct cgaagatatt attttatcag attaaggtaa aaacatttta
    32941 gaataggata acaaaggaga aagtcttgtt gctctctcct tgttgtattt attaaattgt
    33001 cagaaatttt attgacagat tagaaaaata atgttacatt tatatccgca gatatctttc
    33061 gataccaaat ctacatgaaa ggacggggta tgaaactttc tcattattta attggcttac
    33121 ttctactcct agtctttctc tctattagca ttgggaccag tgatttttca tggggaaagc
    33181 tatttgattt cgaccagcag acatggctcc tctttcaaga gtcccgtctc ccaagaacta
    33241 tcagtattct cctgactgcc tctagtatga gtatggcagg ccttctcatg cagactatta
    33301 cccaaaatca gtttgctgca ccgagtacag ttggaacgac tgaagccgcc aaactgggaa
    33361 tggtgctgag cctttttgtc tttccatcgg ctagtctgac ccaaaagatg ctcttcgctt
    33421 ttgtttcatc catcgtattc accctcttct tcctagcctt tatgaccatt tttactgtaa
    33481 aggaaaggtg gatgttgcct ctgattggga tcatctatag cgggattatc ggctcagtca
    33541 cagaagttat cgcctatcgt ttcaatctgg ttcagagtat gactgcctgg acccagggct
    33601 ccttctccat gattcagacc catcagtatg agtggctctt cttaggcctc atcatcctga
    33661 taaccgtttg gaaattatcc caaaccttca ccatcatgaa tctggggaaa gaaatcagcg
    33721 agagtttggg gatttcctac tccctacttg aaaaactggc cctctttctg gtggcgctaa
    33781 cgacaagcgt caccatgatt accgtgggtg gcctaccatt tctcggagtt atcgttccca
    33841 atcttgttcg caagcgctat ggagataatc taagtcaaac caaactcatg gtcgcactgg
    33901 ttggtgccaa tctagttctg gcttgcgata tcctatcccg agttctgatt aggccctatg
    33961 agttgtctgt cagtctcttg ctaggaatca tcggtagtct cggctttatc ctacttctct
    34021 ggagaggggg acgaaaagat gcagactaaa agcaaacata ccaagctctt ctggattctc
    34081 attattcttg ccatcggagc ttgtcttctc tacttttggc ccatcactca cttgtcagcc
    34141 tttgcttgga agttgcgttc ccaaaagatc atcgtttatc tcttggtagc catcgcgact
    34201 gggatttcga ccattagttt tcaaaccctg acggaaaatc gcttcctgac gcctagtatt
    34261 ttgggaattg aatccttcta cgtcctacta caaaccctac tactggtttt tgaaagcaag
    34321 tttcttcaac ttggcaaatc ccctatctta gaattcctag tcttacttct tgtccagtcc
    34381 ctcttctttc tcaccttaca aggttacttg aagacactga tgaagcaaga cctggtcttc
    34441 atcctgctga tctgtctagc gctcagaagt ctctttcgaa atatcagcac cttccttcaa
    34501 gtcctaatgg atccaaacga atacgataaa ctgcaaaata gtctttttgc ctcctttcaa
    34561 catctcaaca cttccatcct agccatcggt tctctgatca tcctcgcttt gacaatcttt
    34621 ttctttcgaa aagcagtcgt tctagatgtc ttgcacctgc aaagagaaac ggctcagata
    34681 ttgggactcg atgttgaaaa agaacagaaa gagctcctct ggggaatcgt gcttttgacc
    34741 tcaacggcca ctgccttggt aggacctatg gccttcttcg gctttatgct ggccaacctc
    34801 acctacctga ttgtcaaaga ctatcagcac aagttactct ttatagtggc cattctggtt
    34861 ggatttatta gcttaacctt ggggcaagcc ttgattgaac gagtctttgc actggaaatt
    34921 cgtatcagta tgatcattga gagtgtgggt ggcttcttat tctttatctt actatatagg
    34981 aggtctcgtc agtgaaactg gaaaacattg acaaatccat tcaaaaacag gatattttgc
    35041 aaggcatttc gcttgaagtc agtcctcaaa aactgacagc ctttattggt ccaaatggtg
    35101 ctggaaaatc gactctcctc tccatcatga gcagactaac caagaaagat cagggagttc
    35161 tcagtatcaa aggacgtgaa atcgagagct ggaattcgca agaactggcc caagaactaa
    35221 ccatcctaaa acagaaaatc aattaccaag ccaaattgac tgttgaagaa ctggtcagtt
    35281 ttggacgttt tccctacagc cgaggtcgac ttagatcaga agactgggaa aaaatccgag
    35341 aaactctgaa ctatttggaa ctgaccaact taaaagaccg ctacatcaat agcctgtcag
    35401 gggggcaact ccagcgcgtc tttatcgcta tggtactggc ccaggatacg gactttatct
    35461 tgctggacga accactcaac aatctcgata tcaagcaaag cgtcagcatg atgcagattc
    35521 ttcgacgact ggtggaggaa ctcggcaaga ccattatcat cgtcctccac gatatcaaca
    35581 tggccagtca gtatgcagat gaaattgtcg ccttcaagga cggccaggtc tttagcaagg
    35641 gaagcaccga tcaaatcatg caggctgacc tgctcagtca actttatgag attcccatca
    35701 cgctagctga tatcaatgac aaaaagatct gtatctatag ctagtaacat aaaagctcaa
    35761 gttagagaac cttcagtctc ttagtcaata agattaagag actccctaaa tcgttatcac
    35821 attttaaaaa ggagaaatta tgaaaacatc ccttaaactt tatttcactg ccctagtggc
    35881 cagcttcttg ctcctacttg gtgcatgtag tacaaactca agcactagtc agacagagac
    35941 cagtagctct gctccaacag aggtaaccat taaaagttca ctggacgagg tcaaactttc
    36001 caaagttcct gaaaagattg tgacctttga cctcggcgct gcggatacta ttcgcgcttt
    36061 aggatttgaa aaaaatatcg tcggaatgcc tacaaaaact gttccgactt atctaaaaga
    36121 cctagtggga actgtcaaaa atgttggttc tatgaaagaa cctgatttag aagctatcgc
    36181 cgcccttgag cctgatttga ttatcgcttc accacgtaca caaaaattcg tagacaaatt
    36241 caaagaaatc gccccaaccg ttctcttcca agcaagcaag gacgactact ggacttctac
    36301 caaggctaat atcgaatcct tagcaagtgc cttcggcgaa actggtacac agaaagccaa
    36361 ggaagaattg gccaagctag acaagagcat ccaagaagtc gctactaaaa atgaaagctc
    36421 tgacaaaaaa gcccttgcga tcctccttaa tgaaggaaaa atggcagcct ttggtgccaa
    36481 atctcgtttc tctttcttgt accaaacctt gaaattcaaa ccaactgata caaaatttga
    36541 agactcacgc cacggacaag aagtcagctt tgaaagtgtc aaagaaatca accctgccat
    36601 cctctttgtc atcaaccgta cccttgccat cggtggggac aactctagca acgacggtgt
    36661 cctagaaaat gcccttatcg ctgaaacacc tgctgctaaa aatggtaaga ttatccaact
    36721 aacaccagac ctctggtatc taagcggagg cggacttgaa tcaacaaaac tcatgattga
    36781 agacatacaa aaagctttga aataagccac ttcaaactca a
//
LOCUS       DAVBCN010000010        35974 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVBCN010000010 DAVBCN010000000
VERSION     DAVBCN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35974
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.010"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            513..1556
                     /locus_tag="VQ497_000492"
     CDS             513..1556
                     /locus_tag="VQ497_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HET2516848.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGHNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFYTESEEQ"
     gene            1723..3810
                     /gene="topA"
                     /locus_tag="VQ497_000493"
     CDS             1723..3810
                     /gene="topA"
                     /locus_tag="VQ497_000493"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET2516849.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            4031..4390
                     /locus_tag="VQ497_000494"
     CDS             4031..4390
                     /locus_tag="VQ497_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HET2516850.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4483..5115
                     /locus_tag="VQ497_000495"
     CDS             4483..5115
                     /locus_tag="VQ497_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HET2516851.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGTLTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQLGVTQLHGTKVVF"
     gene            5159..5815
                     /locus_tag="VQ497_000496"
     CDS             5159..5815
                     /locus_tag="VQ497_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET2516852.1"
                     /translation="MFEIFKSYQFNQEKAHAFGFVVNSGVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETDDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5834..6466
                     /locus_tag="VQ497_000497"
     CDS             5834..6466
                     /locus_tag="VQ497_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET2516853.1"
                     /translation="MNFQLAKYSLLKKFSENIEFTTPGEYGAIFKYLIENVKTDRQII
                     CSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            6494..6928
                     /locus_tag="VQ497_000498"
     CDS             6494..6928
                     /locus_tag="VQ497_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HET2516854.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTINAVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIAYINANTFVQKENACEMGRSVSYHDMPSV"
     gene            6940..7977
                     /gene="leuB"
                     /locus_tag="VQ497_000499"
     CDS             6940..7977
                     /gene="leuB"
                     /locus_tag="VQ497_000499"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET2516855.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADVILLAAIGSPQYDGAAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSA
                     AMLMISNPAKFDVIVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIYGSA
                     PDIAGQGIANPTSMILSVAMMLRDSFGRYEDEERIKRAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            7974..8243
                     /locus_tag="VQ497_000500"
     CDS             7974..8243
                     /locus_tag="VQ497_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HET2516856.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRTWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            8247..8606
                     /locus_tag="VQ497_000501"
     CDS             8247..8606
                     /locus_tag="VQ497_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET2516857.1"
                     /translation="MAGSSREHAAWALANYGFKVVIAGTFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPTDHVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8768..9336
                     /locus_tag="VQ497_000502"
                     /pseudo
     CDS             8768..9336
                     /locus_tag="VQ497_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007122522.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            9430..9495
                     /locus_tag="VQ497_000503"
                     /pseudo
     CDS             9430..9495
                     /locus_tag="VQ497_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            9658..11589
                     /locus_tag="VQ497_000504"
     CDS             9658..11589
                     /locus_tag="VQ497_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HET2516858.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELMSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQ
                     KDQAVADDNESDENDDADY"
     gene            11576..12892
                     /locus_tag="VQ497_000505"
     CDS             11576..12892
                     /locus_tag="VQ497_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HET2516859.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKKQKILETINQEIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEMGNL
                     AGYGALLKKWSIQIPQKSSSYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            13067..14053
                     /locus_tag="VQ497_000506"
     CDS             13067..14053
                     /locus_tag="VQ497_000506"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HET2516860.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKQMHDQGLIASISVGVKDYEY
                     DFVSQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            14117..14188
                     /locus_tag="VQ497_000507"
     tRNA            14117..14188
                     /locus_tag="VQ497_000507"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14150..14152,aa:Arg,seq:ccg)
     gene            14371..15069
                     /locus_tag="VQ497_000508"
     CDS             14371..15069
                     /locus_tag="VQ497_000508"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET2516861.1"
                     /translation="MKELQTVLKNHFAIEFADKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            15060..18599
                     /gene="smc"
                     /locus_tag="VQ497_000509"
     CDS             15060..18599
                     /gene="smc"
                     /locus_tag="VQ497_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET2516862.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKSEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAITVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDAVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLRSEEAALKTLQDQMATLTERLEVIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQDRLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDFEQEEIQRLLQEKVDN
                     LEKVDTELLSQQAEEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRYLQSQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNIEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKDRFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            18596..19390
                     /locus_tag="VQ497_000510"
     CDS             18596..19390
                     /locus_tag="VQ497_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2516863.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYI
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPAAPENARPEILELAKTVIGHHKDQSVIAYMEGL"
     gene            19390..20208
                     /locus_tag="VQ497_000511"
     CDS             19390..20208
                     /locus_tag="VQ497_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2516864.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKATLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDDHEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFADLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAVANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            20212..21489
                     /gene="ftsY"
                     /locus_tag="VQ497_000512"
     CDS             20212..21489
                     /gene="ftsY"
                     /locus_tag="VQ497_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET2516865.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVVEVSQKEVEEFPHSEEGNTEFLATVE
                     ENNSEILEAERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQ
                     DNLTVMLFVGVNGVGKTTSIGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(21535..23022)
                     /locus_tag="VQ497_000513"
     CDS             complement(21535..23022)
                     /locus_tag="VQ497_000513"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET2516866.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYQSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATANKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVCASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDITYRQDGRLE"
     gene            complement(23171..23911)
                     /locus_tag="VQ497_000514"
     CDS             complement(23171..23911)
                     /locus_tag="VQ497_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2516867.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(23911..26076)
                     /locus_tag="VQ497_000515"
     CDS             complement(23911..26076)
                     /locus_tag="VQ497_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET2516868.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            26249..27202
                     /locus_tag="VQ497_000516"
     CDS             26249..27202
                     /locus_tag="VQ497_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2516869.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAYTLYGIS
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            27279..29267
                     /gene="uvrB"
                     /locus_tag="VQ497_000517"
     CDS             27279..29267
                     /gene="uvrB"
                     /locus_tag="VQ497_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HET2516870.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARIEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMTYNEEHDIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKVLD"
     gene            29277..29840
                     /locus_tag="VQ497_000518"
     CDS             29277..29840
                     /locus_tag="VQ497_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2516871.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            29806..30294
                     /locus_tag="VQ497_000519"
     CDS             29806..30294
                     /locus_tag="VQ497_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2516872.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWREAYDDLLPAEFQETMTLE
                     RCRFFSQKYPENTLIAIGDLKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            30368..30835
                     /locus_tag="VQ497_000520"
     CDS             30368..30835
                     /locus_tag="VQ497_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET2516873.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(30832..31347)
                     /locus_tag="VQ497_000521"
     CDS             complement(30832..31347)
                     /locus_tag="VQ497_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HET2516874.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(31359..31886)
                     /locus_tag="VQ497_000522"
     CDS             complement(31359..31886)
                     /locus_tag="VQ497_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2516875.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            complement(32164..32727)
                     /locus_tag="VQ497_000523"
     CDS             complement(32164..32727)
                     /locus_tag="VQ497_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2516876.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(32711..33262)
                     /gene="coaC"
                     /locus_tag="VQ497_000524"
     CDS             complement(32711..33262)
                     /gene="coaC"
                     /locus_tag="VQ497_000524"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HET2516877.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(33274..33963)
                     /gene="coaB"
                     /locus_tag="VQ497_000525"
     CDS             complement(33274..33963)
                     /gene="coaB"
                     /locus_tag="VQ497_000525"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HET2516878.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            34193..35863
                     /locus_tag="VQ497_000526"
     CDS             34193..35863
                     /locus_tag="VQ497_000526"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HET2516879.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELEIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNALSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
BASE COUNT        11122 a         6365 c         7968 g        10519 t
ORIGIN      
        1 taatttatag aaatatttta gcagccaagg tgcactgtta tagattcaat acactataat
       61 ttagtgtaat tgagaaagga gaaatgattg tgattgatgt tggctaggtt atgttcaatg
      121 attcctaccg tctcaaatct tgtcagtaag gaaaaataaa ttcttcaaaa gtagagatta
      181 caaggcttgt ttaagaaaga attcaaagac cttgacaaat aaaaataaaa tggttattat
      241 aaaaaatggt ctgaaataga tgatgatact tttcgaaaat ctttttacgt cagctcagct
      301 ttgccttgct gtgttttgag caagctacgg ttagcttccg agtttgattt tcatttacta
      361 gaaatgaaac tgatgagaga tatcagtaga catttgagtc aggatattat ggaaaatgat
      421 aaaaagagct cgtgagattg gcatatcaga ctactaaagt attgagtttg ttaggatttt
      481 agcgactagt tagctgggaa aggaagatat ttgtgacaaa taataaactg tattcgttga
      541 tagaatttag aaataaaata tatgaagaat tagaactttc cagaagtgat ttagcgattt
      601 tactatgtgc catgcttatc gcctctatcg gattaaatat ggattcgact cccgtgatta
      661 ttggagccat gttaatctct cctttgatga cacctattct gggagtgggg ctctctctag
      721 ctatatttga ttttaaattg ttaagaaaat cttttaaaat attagctatt caaattcttg
      781 ccagtttaat agcttcaaca ctttattttt atctttctcc catttcgtat gctagttcgg
      841 aaattgttgc tagaacctct ccgactattt gggatgttct cattgctttt gtaggaggga
      901 tagcaggtat tattggtgct aggaaaaaag agaccaataa tattgttcct ggtgttgcta
      961 ttgcaaccgc cttgatgcct cctctttgta cagtaggtta tgctattgct tctgctaatc
     1021 taaaatttat cataggctcc tcttacctat tcctcatcaa ttgtagcttt attgtcattg
     1081 cgacttatat aggtgttagg ttgatgatgg ttaagaaaca ttattttaaa gataatgaag
     1141 aagactctaa aatgcgtagg attttgcttc tagttgctgt tttgctgatg attccgagtt
     1201 tcatctctgc aacgacttta gtgagagaaa cgttgaaaaa agagtccctt aagaaattta
     1261 tatcagagca gtttcagggg cataatattt tgaaaaaaac ctattctaaa aagactcata
     1321 ccctaaagct aaccatttca ggaaattatt tgacagaaga agaactcgat atgatttcaa
     1381 gtaagagagg tgactatggt ttaagtgatg tttctgttca agtttcacaa ttgtctgatt
     1441 cagaacaact tagtaaggaa gaactggtgg agtatttctt ccagtatatc aaggataagg
     1501 aagcaaaaga aaaggaaaaa gctaataagt tttatacaga gtctgaggag caataatttc
     1561 ttgagaatag ctggtttctc tcgtgagtct tctatgtata tcaaaggaag actgaggttt
     1621 taagtataaa acttttcttc tattatagta gctaagtctt gacagggttc aaaaaatggt
     1681 ttacacttta taaagtttat tactttgaaa aggtgtgata ctgtggctac ggcaacaaaa
     1741 aagaaaaaat caacagttaa aaaaaatcta gtcatcgtgg agtcgcctgc taaggccaag
     1801 acgattgaaa aatatctagg cagaaactac aaggttttag ccagtgtcgg gcatatccgt
     1861 gatttgaaga aatccagtat gtccgtcgat attgaaaata attatgaacc gcaatatatc
     1921 aatatccgag gaaaaggccc tcttatcaat gacttgaaaa aagaagctaa aaaagctaat
     1981 aaagtttttc tcgcgagtga cccggaccgt gaaggagaag cgatttcttg gcatttggcc
     2041 catattctca acttggatga aaatgatgcc aaccgtgtgg tcttcaatga aatcaccaag
     2101 gatgcagtca aaaatgcttt taaagaacct cgtaagatcg atatggactt ggtcgatgcc
     2161 caacaagctc gtcggatctt ggatcgcttg gtagggtatt cgatttcgcc tattttgtgg
     2221 aagaaggtca agaagggctt gtcagcaggt cgcgttcagt ccattgccct taaactcatc
     2281 attgaccgtg aaaatgaaat caatgccttc cagccagaag aatactggac agttgatgct
     2341 gtctttaaaa agggaaccaa acaatttcat gcttccttct atggagtaga tggtaaaaag
     2401 atgaaactga ccagcaatga cgaagtcaag gaagtcttgt ctcgtctgac tagtaaagac
     2461 ttttcagtag atcaggtgga taagaaagag cgcaagcgca atgctccttt accctatacc
     2521 acttcatcta tgcagatgga tgctgccaat aaaatcaatt tccgtactcg aaaaaccatg
     2581 atggttgccc aacagctcta tgaaggaatt aatattggtt ctggtgttca aggtttgatt
     2641 acctatatgc gtaccgattc gactcgtatc agtcctgtag cgcaaaatga ggcggcaagc
     2701 ttcattacgg atcgttttgg tagcaagtat tctaagcacg gtagcaaggt caaaaacgca
     2761 tcaggtgctc aggatgccca tgaggctatt cgtccgtcaa gtgtctttaa tacaccagaa
     2821 agcatcgcta agtatctgga caaggatcag ctcaagctat atacccttat ctggaatcgt
     2881 tttgtggcta gccagatgac agcggccgtt tttgatacca tggctgttaa attgtctcaa
     2941 aatggagttc aatttgctgc caatggtagt caggttaagt ttgatggtta tcttgccatt
     3001 tataatgatt ctgacaagaa taagatgtta ccggacatgg ttgttggaga tgtggtcaaa
     3061 caggtcaata gcaaaccaga gcaacatttc acccaaccgc ctgcccgtta ttctgaagca
     3121 acactgatta aaaccttaga ggaaaatggg gttggacgtc catcaaccta tgcgccaacc
     3181 attgaaacca ttcagaaacg ttattatgtt cgcctggcag ccaaacgttt tgaaccgaca
     3241 gagttgggag aaattgtcaa taagctcatc gttgaatatt tcccagatat cgtaaacgtg
     3301 accttcacag ctgaaatgga aggtaaactg gatgatgtcg aagttggaaa agagcagtgg
     3361 cgacgggtca ttgatgcttt ttacaaacca ttctctaaag aagttgccaa ggctgaagaa
     3421 gaaatggaaa aaatccagat taaggatgaa ccagctggat ttgactgtga agtgtgtggc
     3481 agtccaatgg tcattaaact tggtcgtttt ggtaaattct acgcttgtag caatttccca
     3541 gattgccgtc atacccaagc aatcgtgaaa gagattggtg ttgagtgtcc aagctgtcat
     3601 cagggacaaa ttattgagcg aaaaaccaag cgtaatcgcc tattctatgg ttgcaatcgc
     3661 tatccagaat gtgaatttac ctcttgggac aagcctgttg gtcgtgactg tccaaaatgt
     3721 ggcaacttcc tcatggagaa aaaagtccgt ggtggtggca agcaggttgt ttgtagcaaa
     3781 ggcgactacg aggaagaaaa gattaaataa gaggaggggc ccgaaagtaa atttcaggct
     3841 ccttttggtt agaacttgac aaaatacatc attttatgcg aaactagaag aagattattt
     3901 tatactcaat gagaatcaaa gagcaaatta gaaagctagt cgcaggttgc tcaaagcact
     3961 actttgaggt tgaagatgga agctgacctg gtttgaagag attttcgaag agtattaact
     4021 aggagaagtt atgcgtctta tctatctgat aattggtttt ttatcactgg ccttggctat
     4081 tgttggggtt gttttaccct tgttgccgac aacgcctttc cttttgttgt ctattgcttg
     4141 tttctccaga agttccaagc gtttcgaaga ctggctttat cataccaagc tctatcaaac
     4201 atatgtagct gattttcgcg agactaagtc tattacgcgt gaacgaaaga aaaaaatcat
     4261 cgtctctatc tacgtcttga tgggaatttc tatttatttt gcacctcttt taccagtcaa
     4321 aatcggtctg ggtgctttga ccatctttat tacttattat ctcttcaagg tcattccaga
     4381 caaagaatag ttaaaacagt agttatttgc cttgataaaa ttgaaagcat attcataaca
     4441 atatgatata ataaatttga agtaatattc aaggagaatc aaatgattta cgaattttgt
     4501 gctgaaaatg tgactttact tgaaaaagcg atgcaggctg gagctcgtcg gattgaactc
     4561 tgtgataatc tagcagttgg tgggacaaca cccagctatg gagtgactaa ggcagcggtt
     4621 gaactggcag ctaactacga tacaaccatc atgaccatga ttcggccacg tggtggtgac
     4681 tttgtctata atgacctaga aattgctatc atgctagaag acattcgttt gactgctcag
     4741 gctggaagtc aaggggttgt atttggaact ttaactgctg ataaaaagtt ggataagcct
     4801 aatctggaaa agttaattgc tgcatcaaaa ggaatggaaa ttgtctttca catggccttt
     4861 gatgaactaa gtgatgaaga tcaaccggaa gctattgact ggctcagtca agccggtgtc
     4921 actcgtatcc taactcgtgc tggtgtgtct ggcgactcct tagaaaaacg ttttgttcac
     4981 tatcacagaa ttttggagta cgctaaaggt aaaattgaaa ttctaccagg tggggggatt
     5041 gaccttgaaa accgtcaaac ctttatcgac cagttggggg taacacaatt gcatggtact
     5101 aaggttgttt tttaaaaaat agaaaggaac tgctagcttt gggtagcagt tttcacttat
     5161 gtttgaaatt tttaaatcct atcaatttaa tcaagaaaag gctcatgctt ttggttttgt
     5221 agtaaatagt ggagtctgga cctatagttg ccagattttg caaggtgact ttgtcatgac
     5281 tgtgtccatc actgctgata atgtgaactt tcaagtcttt gaccaagaga ctgatgacct
     5341 ctatcctcac gttcatatgg aaagcatgag gggaagtttt gtcggaaatg tccgtgaggc
     5401 ttgtctggag attctttacc agattcggaa ggcttgtttt gatgtgcaag attttatctg
     5461 tcatcagact aagcgtatta tgactcaagt tcaggaaaag tatggaaacc agttggagta
     5521 tctgtgggaa aaatcgcctg atacagctgt attgcgccat gaaggcaatc aaaagtggta
     5581 tgccgtcttg atgaaaatct cttggaataa gctggaaaag ggcagagaag gacaagtgga
     5641 agcagtcaac ctcaagcatg accaagtagc taatttactt tcacaaaagg ggatttatcc
     5701 agcctttcat atgagcaagc gctactggat tagtgtgtcc cttgatgata ctttatcaga
     5761 tgaagaagta ctggaattga tagaaaaaag ttggaactta acctctaaaa aatgaaatat
     5821 tttaataatt ttcatgaact ttcaattagc taaatattct ttactgaaga aattttcaga
     5881 aaatatagaa tttaccacac caggggaata tggtgccatc ttcaaatacc tgattgagaa
     5941 tgtcaagacg gatcgtcaga tcatctgttc gcctcactgt catgatgacc tcggaatggc
     6001 agtggcaaat agccttgctg ctgtcaagaa tggtgcagga cgtgttgaag ggactatcaa
     6061 tggtattagg gagcgagctg aaaatgctgc tttggaagaa attgcagtgg ctctcaatat
     6121 tcgccaagat tactaccaag tagaaaccag tattgtccta aatgagacca tcaatacgtc
     6181 agaaatggtt tctcgcttct ctggtattcc agttcctaaa aacaaagccg tcgttggtgg
     6241 caataccttc tcccatgaat ctggtattca ccaagatgga gtccttaaaa atcctctcac
     6301 ttatgagatc atcacacctg aattggttgg tgttaagatt ccgcttggaa aattatctgg
     6361 tcgccatgct tttgttgaga aactgagaga attggcccta gattttacag aagaggatat
     6421 caaaccactc tttgctaagt tcaaggcact ggccgataag aagtaagaaa tcacagatgc
     6481 agatactcga gctttggtag ctggaaccat ggttgaaaat ccagaaggct tccactttga
     6541 tgatttacaa cttcaaactc atgcagataa tgacattgaa gcgctcgtta gcctagccaa
     6601 tatggatggt gagaaagtcg aatttaatgc gacagggcaa ggttccgttg aagcaatctt
     6661 taacgctatc gataagttct ttaaccaatc tgttcgtttg gtgtcctaca ctatcaatgc
     6721 ggtaacagat ggaatcgatg cccaggatcg ggttttggtc actgttgaaa acagagatac
     6781 agaaaccatc tttaatgcag cagggcttga ttttgatgtg ttgaaggctt ctgctattgc
     6841 ctatataaac gctaatacct ttgttcaaaa agagaatgca tgtgagatgg gacgcagtgt
     6901 ttcttaccac gatatgccta gtgtgtaaag gagaaggcta tggcaaagaa aatagtagct
     6961 ctagtaggag acggaattgg cccagaaatc atggaggctg gtttagaagt tctggaggct
     7021 ctagctgaaa aaacaggttt tgactatgag attgacagac gaccgttcgg aggtgcagat
     7081 attgatgcag caggacctcc cttacctgat gaaaccctta aggcaagtag ggaagcagat
     7141 gttatcctac tagcagctat cggtagtcct cagtatgatg gagcagcggt tcgccctgaa
     7201 caaggcctga tggctctccg taaggaactc aatctttacg ctaatattcg tcctgtaaaa
     7261 atctttgaca gtctcaagta tttgtcacca cttaaaccgg aacgaatttc tggtgtagac
     7321 tttgtcgtgg tgcgtgaatt gacaggcgag atttactttg gagatcatat tcttgaagag
     7381 cgcaaagcgc gtgatatcaa cgactatagc tatgaggaag tggagcggat tattcgcaaa
     7441 gcctttgaaa ttgcaagaaa tcgcagaaaa atcgttacta gtatcgataa gcaaaatgtt
     7501 ctagcgacct caaaactctg gcggaaagta gctgaggaag tcgcacagga tttcccagat
     7561 gtaaccttgg aacaccagct ggtagactca gctgctatgc ttatgattag caatcctgct
     7621 aagtttgatg ttattgtaac ggagaatctt tttggagata ttttatctga tgaatcaagc
     7681 gtcttatctg gtacacttgg ggttatgcca tcagccagtc attctgaaaa tggaccaagt
     7741 ctctatgaac ctatttacgg ttcagcacct gatattgcag gtcaaggaat tgccaatcct
     7801 acttccatga ttttatcagt tgccatgatg ttgagagata gtttcggacg ttatgaggat
     7861 gaagagcgta tcaaacgtgc tgttgagaca agtctggcgg caggaatttt aacgagagat
     7921 ataggggggc aggcttcaac aaaggaaatg acggaagcta ttattgcaag gttatgaagt
     7981 tagacgaaaa aattactcta gtccttttga tttggaatgt catcattttc ttgatttatg
     8041 gcattgacaa attcaaggca agaagaagaa cttggcgcat ccaagagaaa atcttactca
     8101 ttttagccct tacttgtggt ggttttgggg cctggttagc tggaatcact tttcaccaca
     8161 agactcgaaa atggtacttt aaaacagttt ggtttcttgg gatggtgacc acactagtag
     8221 ccttatattt tatttggagg taatggatgg cagggtcttc gagggaacac gctgcttggg
     8281 ctctagcgaa ctatggtttt aaggtcgtga ttgcaggaac tttcggtgac attcattaca
     8341 ataatgaact caataatggc ctgttgccaa tcgttcagcc tagagaggtt agagagaaac
     8401 tagcccagct aaaaccaacc gaccatgtaa ctgtggactt ggaacaacaa aaaatcatct
     8461 caccagttga agaattcacc ttcgagatag atagcgagtg gaaacataaa ctcctaaata
     8521 gtttggatga tatcggtatt accttgcagt atgaagagtt gattgctgct tatgaaaaac
     8581 aacgaccagc ctactggcag gattagaaaa aatagaaaag gaaatatagt aaactgaaat
     8641 aagatgtaaa caaatgaatt agagcttaac atccatttcc agcaattttt tagaaactac
     8701 agtggactat tctggattca acacattata aaattatgac aagacacatt cacaagaagg
     8761 ctacgacatt ttaaaaggtg agggcggatg tatcgtttgc cctactaaag ttggttacat
     8821 tatcatgacc agtgacaagg caggacttga gcgtaagttc gcagccaaag aacgtaagcg
     8881 taacaaacca ggtgttgttc tctgcggtag cattgatgaa ctttgcgctt tagcgcaact
     8941 caacccagaa attgaagcat tctactaaaa acattgggat gaagatattc ttcttggttg
     9001 tatccttcct tggaaaccag aagcctttga aaaactcaaa gcatacgggg atggccgtga
     9061 agaacttatg actgatgtac gtggtactag ctgttttgtt atcaagtttg gaaaagcagg
     9121 tgaacaattg gctgccaagc tttgggaaga aggtaaaatg gtctacgcct catctgcttc
     9181 aatgacaaaa cgattgaaac tcgctatgag caaggtgtaa tggtgtctat ggtcgataag
     9241 gacggcaaac tcatcccaga acaaggagga gcacgttcaa cttcaccagc tccagttgtg
     9301 atccgtaaag ggcttgacat tgatatagta aaatgaagta agagcaggac aaattaatca
     9361 ggacagtcaa atcgatttct aacaatgttt tagaagtaga ggtgtactat tctagtttca
     9421 atccactata aaatcatgat gcacctgtca gatactttta actcatggga ctaccgtcag
     9481 gttgagtatt attaggatag agaagaagtc tagtgttaag gacattacag ctcataatac
     9541 taggcttttt tatagaaatt cttttctttt tctataggat atggtattct atgtagaaaa
     9601 tatatgttaa taagtaatgc caatatttaa acatcattag taaaaggagt tagattgatg
     9661 aataaaagaa aagttagttt agaagatttt tataaatggt atagtctaaa taaagaagag
     9721 ttattaaata aggcaactgt tggtgaaaag tttaatgata aattaaaaga agagtttctc
     9781 caggaatggc ctttggatag gattttaaca atgtcaatcg atgaatatgt aataggaaag
     9841 ggacagcaaa ataagtcttt atgctacgct cttgagaagg gaaaatacaa aaatctattt
     9901 cttggaattt ctggtggctc agcttcaaaa tttggtattt attggaataa aaaaacaaac
     9961 aaatataaag atcaagctaa taatgagatt tcagagttgg atcagcgatt ttcaaaatta
    10021 aaatcagatt tgtatgaaat tatcaaagaa ggtattcgtt ttaactttga aaatcctatt
    10081 tttgatatga aaagatcaac aaatgaattt attggtcgtt ctgctatggt gacaaaatta
    10141 ctttgtatct atactgaggg agatcctttc tttggtgtaa atattaatag tcagaaagaa
    10201 ttttggaacc actttgtttc tcagacaaat caaggtggac cttatctgca aaatcataaa
    10261 ataattgaac tgatgtccaa aacttatcct gagttggagc catcgaaatt aggaactatg
    10321 ctttttgagt attctaagct ttttatggaa aataaggaag acaatagtac aatggattca
    10381 tcaaacaatt ttcgtcatca attaactcaa tctctattaa agtctccaaa ccttatcctt
    10441 cgtggtgctc ctggcacagg aaagacttat cttgctaaag aaattgccaa agaattaacg
    10501 gatggcaacg aagatcaaat cggatttgta caatttcacc catcatatga ttatacggat
    10561 tttgtagaag gtttaagacc agtatcaaat ggggatggag ctattgagtt taggctacag
    10621 gacggtattt ttaaagattt ttgtcagaaa gcaaaagaaa cccaattgat tggaggacaa
    10681 gataattttg atgaggcttg ggattcttac ttagaatata taaatgttgc tgaagaaaaa
    10741 gaatatataa caaaaacatc ttacttatct gttaatagta gacaaaattt gtcagtaaat
    10801 tatgatagtg gtgttccagg atggtcacta cctagcaaat atgtttacga gttgtataaa
    10861 gataaaaatt ataataagca agaatactac aaaagtggtg gaaaaactgt cctagaaaca
    10921 ttgagaaaga gatttggttt gaaagactat gtttccccaa cagaaattga tactgataag
    10981 aaattcgttt tcatcatcga tgaaatcaat cgtggggaga tttctaagat ttttggcgaa
    11041 ctctttttct ctatcgaccc tggctatcgt ggtgaaaaag gaagtgtttc tacccaatat
    11101 gcaaatctac acgaaactga tgaaaagttc tatatccccg aaaatgttta catcatcgga
    11161 actatgaatg atattgatcg ttcagtggat acctttgatt ttgctatgcg tcgtcgtttt
    11221 cgttttgttg aagttactgc cgagggtcaa gttggcatgt tggataaaga gctgaatatc
    11281 catgcagaag aagcaaaaat tcgtctaaga aacttgaacg ctgctatcga aaatgttcag
    11341 gaattaaaca gtcattatca tattggacca agttattttc ttaagttgaa ggatgtagat
    11401 tttgactatg aattactctg gtccgattat attaagcctc tcctagaaga ctacttgcga
    11461 ggttcttatg atgaggttga aactttggaa actttgaaaa aagcatttga actgacaaat
    11521 aatgagcaaa aagatcaggc agtagctgat gacaatgaaa gcgatgaaaa cgatgatgcg
    11581 gattactgat aatcaacaca agattattaa agaaaaattt gttgaagaat atcctaaact
    11641 aagcaatctt cttttagaca gaaccttgga aagtctatcc caagatgaac gtattttcat
    11701 ttttccaaat gatttgactc atactccaga tttggataaa aaacaaaaga ttttggaaac
    11761 gatcaatcag gaaatcaaga caggaaatgt gattggtttt cttggatatg gtcaggaaag
    11821 attaacgatt tcctcacgat tttctgatga gagtaatgac cattttttgc attatctctt
    11881 aaacaaggtt cttcatatca atctcactag tttagatgtt gctttgtctc gtgaagagag
    11941 gctttatcaa cttttgatgt atctcttccc caagtatcta caagctgcta ttcgaaaagg
    12001 tctttataag gaatatcatc gattttctca taacgacagt catgttaagg gagtgattga
    12061 tgtaagaaac catctcaaga aaaatcttcc tttcacggga aatattgcct acacaacgag
    12121 agagttcacc tatgataatc ccctcatgca gttggtccgt cacactattg aatacattaa
    12181 gaatcagaaa agcattggtc aaggggtact agataatctc tcaactagtc gtgaaaacgt
    12241 atctgaaatc gtgcgtgtaa cgccctctta taaactagct gatcgtgcta agattattcg
    12301 gggaaatcaa tctaaaccta tacgtcatgc atactttcac gagtacagaa acttacaaga
    12361 actttgtctg atgatcctaa accaagaaaa gcacggttta gggtatcaag atcaaaaaat
    12421 ctatggtatt ctctttgatg ttgcctggct ttgggaagag tatgtttaca ccttgttgcc
    12481 aaaaggtttt gtacatccca gaaataagga taagacggat ggaatttcag tattttctgt
    12541 tgggaaacga aaagtatatc cagattttta tgacagagaa cgaaagattg ttctagatgc
    12601 aaaatataaa aaactggaat tgactgaaaa aggaatcaat cgtgaggact tattccagct
    12661 gatttcctat tcttatattt taaaagctga gaaggctgga cttgtttttc cgagcaagga
    12721 caaggttatt gataatgaga tgggaaacct agcaggttat ggagctttgt tgaagaaatg
    12781 gtctatccaa attcctcaga agtcttcatc ttatagtgca ttttgtaaaa tgatggaaaa
    12841 ttcagaagaa aattttaaag cgattattga tgaagaagtg gggagaaaat aatttcccct
    12901 ctttctttta tttttaacac aagtaaaacc cgaacaatat atttcaattc ataaaataat
    12961 ttgacaaaaa ctgtactttg gtttataata aattaaggaa acgtcggaaa agacgtagtg
    13021 atgcgagagc ataggtaggt cattacaaaa gaaacgagac atcgatatgt taaatgaatt
    13081 tccaattttt gattacgaag atattcaatt gattccaaat aaatgtgtca ttaaaagccg
    13141 tgcagaagcg gatacaagtg tcactttagg aaatcacacc tttaaactac ctgttgtgcc
    13201 agcgaatatg cagacgattt tggatgaaaa tgtagcagag caactggcta aaggtggtta
    13261 cttctacatt atgcaccgtt ttgatgaggc aggacgcatt ccttttatta aacaaatgca
    13321 cgatcaaggg ctcattgctt ccatctctgt cggtgttaag gactatgagt atgattttgt
    13381 tagccagctc aagactgatg ctcctgagta tatcactatt gatattgctc atggtcatgc
    13441 ggatagcgtg atttctatga ttcaacatat caagaaagaa ttgccagata cctttgtcat
    13501 tgctggaaat gtgggaacac cagaagctgt gcgtgaattg gaaaatgctg gtgcggatgc
    13561 tactaaggtc ggaatcggtc ccggtaaggt ttgtatcacc aaggttaaga ctggttttgg
    13621 tacaggtggt tggcagttgg ctgctctacg ctggtgtgcc aaggctgcgc gtaaaccgat
    13681 tatcgctgat ggaggaattc gtactcacgg tgatattgct aagtctatcc gcttcggtgc
    13741 tagcatgatc atgattggtt ccctctttgc aggacacatt gaaagtccag ggaaaacgat
    13801 tgaagtcgat ggtgaacagt tcaaagaata ttatggttca gcctcacaat atcaaaaagg
    13861 tgcttacaaa aatgtggaag gcaaacgtat cttgcttcct gctaaaggtc atctgcaaga
    13921 cactttaact gagatggaac aagaccttca aagtgctatt tcttatgcag gtggacgtca
    13981 ggttgctgac cttaaacacg ttgattatgt gatcgtgaaa aactccatct ggaatgggga
    14041 tgcttcccac taagacgggg tctttgacca aaaaacttgt tattagagca aatttctgtt
    14101 ataataaaac aagtttccac ccttagtgta atggatatca cgtaagattc cggttcttga
    14161 gatgggggtt cgattccctc agggtggatg taaacatcct aaaaagcctt taatagggct
    14221 tttttcttta tcctgaccca aatttgccgc taaaactgag aatctctgtc taaagaattc
    14281 ccaaatgctt tccagtcccg ttttagagtg actcaggggg ctttttttga tataataaaa
    14341 aggactgtta tcagttagaa agaggttggt atgaaagaat tacaaactgt actaaagaat
    14401 cattttgcaa tcgaatttgc agacaaaaat ttactggaaa ctgcctttac tcatacgagt
    14461 tatgccaatg agcaccgcct cttaaaaatt tcacacaatg aacgcttgga atttttagga
    14521 gacgctgttc tacagttatt gatttcagaa tatctatata aaaaatatcc taaaaagcct
    14581 gaaggtgacc tatcaaaact ccgtgctatg attgtccgtg aggagagttt agctggtttt
    14641 gcgcgtgatt gccagtttga ccagtttatc aagttgggta aaggggaaga aaaatcaggt
    14701 ggtcgcaatc gtgacaccat tcttggtgat gcctttgaag cctttcttgg tgcccttctt
    14761 ttggataagg atgtggccaa ggtcaaggaa tttatctatc aagtcatgat tcctaaggtt
    14821 gaagcaggcg aatttgagat gattacagac tataaaaccc atctccaaga gttacttcag
    14881 gtcaatggtg atgtggctat tcgttatcag gtgatttctg aaacagggcc tgctcacgat
    14941 aaggtctttg atgtagaagt tcttgttgaa ggtaagagca tcggtcaagg ccaaggtcgt
    15001 tctaagaaat tagcagagca ggaagctgcc aaaaatgccg ttgagaaggg gctggattca
    15061 tgtatttaaa ggaaatcgaa attcaggggt tcaagtcttt tgctgataag accaaggtcg
    15121 tttttgacca aggtgtgacg gcagttgttg gacccaatgg atctggaaag tccaatatta
    15181 cagaaagtct gcgttgggct ttgggggagt ctagtgtcaa gagtctccgt gggggcaaga
    15241 tgccggatgt catctttgct ggaacagaaa gtcgcaaacc gctcaattat gcttctgtag
    15301 ttgtgactct ggataatcat gacggattta tcaaggatgc aggtcaagaa atcagggtgg
    15361 aacgccatat ctatcgtagt ggagatagcg aatacaagat tgacggcaag aaagtccgtc
    15421 tgcgtgatat tcatgacctt ttcttggata caggtttggg acgagattcc ttctctatta
    15481 tttcccaagg gaaggttgag gagattttta attccaagtc tgaggaacga cgagctattt
    15541 ttgaagaagc tgctggagtt ttaaaataca agactcgcag aaaagaaacc gagagtaaac
    15601 tgcagcaaac tcaggataat ctggaccgct tagaggacat tatctacgag ttggataatc
    15661 aaatcaagcc tcttgagaag caagctgaga atgcccgtaa atttctagac ttagaaggtc
    15721 aacgcaaggc tatttacttg gatgtattgg ttgctcaaat caaggaaaat aaggcagaac
    15781 tagagtcgac agaagaagag ttggctcagg ttcaagaact cttgatgagt tattaccaaa
    15841 agcgtgaaaa attagaagaa gaaaatcaaa ctcttaaaaa gcaacgccaa gatttacagg
    15901 ctgaaatggc caaagaccaa ggcagtttga tggacttgac tagtctgatt agtgatttag
    15961 aaagaaaatt agccctatcg aaactggagt ctgagcaagt ggccttgaat caacaggagg
    16021 cacaagctcg tttggctgct ttggaggata agagaaattc actcagcaaa gaaaagtctg
    16081 ataaagaaag ctctttagct ctgttagagg gaaatctagt ccaaaataat caaaaactca
    16141 atcgtttaga agctgaattg ctggctttct cagacgatcc tgatcagatg attgagctct
    16201 tacgtgaacg ctttgtagct cttttacaag aagaagcgga tgtctcaaac cagttgaccc
    16261 gtattgagaa tgagttggaa aatagtcgtc agctttctca aaaacaagca gatcaactag
    16321 aaaagctgaa agagcaatta gctatagcta aagagaaggc tagtcagcaa aaggaagagc
    16381 ttgaaactgc caaggagcag gttcagaaat tattggctga ctaccaagct attgccaagg
    16441 agcaagagga gcagaaaact tcctatcaag ctcaacaaag ccaactcttt gaccgtctgg
    16501 ataatctcaa aaacaagcag gccagagctc aaagtttgga aaatatcctg agaaatcata
    16561 gtaactttta tgcaggtgtt aagagtgttc tccaagaaaa agatcgccta ggtgggatta
    16621 ttggtgcagt cagtgagcat ctgacctttg atatgcatta tcaaactgcc ctagaaattg
    16681 cgcttggagc gagtagccaa catatcatcg tagaagatga agagtcggca accaaggcta
    16741 ttgacttcct taaacggaac agagccggtc gtgcaacctt tcttcctttg accactatca
    16801 aggcgcgtac gatttctagt cagaaccaag atgctatcac tgtaagccca ggtttccttg
    16861 ggatggcaga tgagttggtg acttttgata ctagactgga agccattttc aagaacttgc
    16921 tagctacgac ggctatcttt gatgccgtag aacatgcgcg tgcagcagct cgccaagttc
    16981 gttatcaggt tcgtatggtg acattggatg ggacagaatt acgcacgggt ggttcctatg
    17041 cgggtggtgc caatcgccaa aataacagta ttttcatcaa gccagaactg gagcaattac
    17101 aaaaagaaat tgctgcagat gaagcaagct tgcgttcaga agaagcggct ttgaagacct
    17161 tacaagacca gatggctaca ttgacagaaa gattagaagt catcaaatct caaggagaac
    17221 aggcacgtat tcaggagcaa ggcttgtccc tcgcttatca gcaaactagt cagcaagttg
    17281 aagaactgga aactctttgg aaactccaag aagaggaaat agatcgtctt tccgagggag
    17341 attggcaagc ggataaggaa aaatgccaag atcgtcttgc tgcaatcgcc agtgacaagc
    17401 aaaatctgga agctgagatt gaagagatta agtctaacaa aaatgccatc caagaacgct
    17461 atcaaaactt gcaggaagag ctagcgcaag ctcgtttgct taagacagaa ctgcaagggc
    17521 aaaaacgtta tgaagttgct gatattgaac gcttaggcaa ggaattggac aatcttgatt
    17581 ttgaacaaga ggaaatccag cgccttcttc aagaaaaggt tgataatctt gagaaggttg
    17641 atacagaatt gctcagtcaa caggcggaag aagccaaaac tcagaaaaca aatctccaac
    17701 aaggtttgat tcgcaagcag tttgagttgg atgatattga aggtcaactg gatgatattg
    17761 ctagtcattt ggatcaggct cgccaacaga atgaagagtg gattcgcaag caaacacgtg
    17821 ctgaagccaa gaaagaaaag gtcagcgagc gcttgcgcta tttacaaagt caattaacag
    17881 accaatatca gattagctat actgaagctc tagaaaaggc gcatgaattg gaaaacctca
    17941 atctggcaga gcaagaggtg caggatttag agaaagctat tcgctcactg gggcctgtca
    18001 atatagaagc tattgaccag tacgaagaag ttcacaatcg tctggatttc ctaaatagcc
    18061 aacgagatga tattttgtca gcgaaaaatc tgctccttga gaccattaca gagatgaatg
    18121 atgaggtcaa agatcgcttt aaatcaacct ttgaggccat tcgtgaatcc tttaaagtga
    18181 ccttcaaaca gatgtttgga ggaggtcagg cagacttaat cttgactgag ggagacttgt
    18241 tgacagctgg ggttgagatt tctgttcaac ctccaggtaa gaaaatccag tcgcttaacc
    18301 tcatgagtgg tggtgaaaaa gccttatcag ctcttgcttt gcttttttct atcattcgag
    18361 ttaagactat cccatttgtc atcttggatg aggtagaggc cgcgctggac gaagccaatg
    18421 ttaaacgttt tggggattac ctcaatcgat ttgacaagga cagccagttt atcgtcgtaa
    18481 cccaccgtaa gggaaccatg gcagcggctg attctatcta tggagtgacc atgcaagaat
    18541 cgggggtctc aaaaattgtt tcggttaagt taaaagattt agaaagtatt gaaggatgac
    18601 aattaaacta gtagcaacgg atatggacgg aaccttccta gatgggaatg gacgctttga
    18661 tatggatcgt ctcaagtctc tcttggcttc ctacaaggaa aaagggattt actttgcggt
    18721 ggcttcgggt cggggatttc tgtctctgga aaaattattt gctggtgttc gtgatgacat
    18781 tattttcatc gcggaaaatg gcagtttggt agagtatcaa ggtcaggact tgtatgaagc
    18841 gactatgtct cgtgactttt atctggcaac ttttgaaaag ctgaaaacgt caccttatat
    18901 agatatcaat aaactgctct tgacgggtaa gaagggttca tatgttctag atacggttga
    18961 tgagacctat ttgaaagtga gtcagcatta taatgaaaat atccaaaaag tagcgagttt
    19021 ggaagatatc acagatgaca ttttcaaatt tacaaccaac ttcacagaag aaacgctaga
    19081 agctggtgaa gcttgggtca atgataatgt ccctggtgtc aaggctatga caactggctt
    19141 tgaatctatt gatattgttc tggactatgt cgataagggt gtagctattg ttgaattagc
    19201 taaaaaactt ggcatcacaa tggatcaggt catggctttt ggagacaatc ttaatgactt
    19261 acatatgatg caggttgtgg gacatcctgc agctcctgaa aatgcacgac cagagatttt
    19321 agaattagca aagactgtga ttggtcacca taaggaccag tcggttatag cttatatgga
    19381 gggcttataa tggcagatat aaaattaatc gcattggact tggacggtac cttgctgact
    19441 actgataaaa ggctgacgga tcgcaccaag gcaactttga aagctgcgcg tgatcgtggt
    19501 atcaaggtcg tattgacaac tggtcgtccc ttaaaagcca tggacttctt tctccatgag
    19561 ttagggactg acgaccacga agatgagtat accattactt ttaatggtgg attagttcag
    19621 aaaaatacag gagaaatcct tgataaaaca gtcttttcat atgatgatgt ggcacgtttg
    19681 tatgaagaaa cagagaaatt atcactgcct cttgacgcta tctcagaagg aactgtttat
    19741 caaattcaat cggaccaaga aagtctttat gccaaattca atccagcttt gacctttgtt
    19801 ccagtggact ttgcagatct gtctagtcaa atgacctaca acaaatgcgt gactgccttt
    19861 gctcaagaac ccttggatgc agccattcag aagatttctc cagaattgtt tgaccagtat
    19921 gaaatcttta aatcacgtga aatgttgcta gagtggtcac caaagaatgt tcataaagca
    19981 acaggcttgg caaaactgat cagccatctt ggaattgatc aaagtcaagt gatggcctgt
    20041 ggtgatgaag ccaatgacct ctctatgatt gaatgggcag ggctcggtgt tgctatgcaa
    20101 aacgctgttc ctgaagtcaa ggctgtcgca aatgtagtga cgccaatgac caacgatgag
    20161 gaagctgtcg cctgggctat cgaagaatac gtgctaaagg agaactaaga tatgggattg
    20221 tttgaccgtc tattcggaaa aaaagaagaa cctaaaatcg aagaagttgt aaaagaagct
    20281 ctggaaaatc ttgatttgtc tgaagatgtt gatcctacct tcacagaagt tgaggaagtt
    20341 tctcaggaag aagcagaggt tgaaattgtt gaacaagctg tgttccaaga agaggaaatc
    20401 caagacacag ttgaagaaag tctggattta gagccagttg tagaagtttc tcaaaaagaa
    20461 gtcgaagaat ttccacactc agaagaaggg aatactgagt ttctagcgac tgtagaagaa
    20521 aataattctg aaattcttga agcagaaagg cctcaaacag aagaaactgt tcaggaaaaa
    20581 tatgaccgca gtcttaagaa aactcgcaca ggtttcggtg cccgcttgaa tgccttcttt
    20641 gctaacttcc gctctgttga cgaagaattt ttcgaggaac tggaagaact gttgattatg
    20701 agtgatgttg gtgtccaagt cgcttctaac ttaacggagg aactacgtta cgaagccaag
    20761 cttgaaaatg ccaagaaacc tgatgcactt cgtcgtgtca tcattgagaa attggttgag
    20821 ctttatgaaa aggatggtag ctacgatgaa agcatccact tccaagataa cttgacagtt
    20881 atgctctttg ttggtgtgaa tggtgttggg aaaacaactt ctatcggaaa actagcccac
    20941 cgctacaaac gagctggtaa gaaggtcatg ctggttgcag cagatacctt ccgtgcgggt
    21001 gcagtagctc agctagctga atggggccga cgagtagatg ttccagtagt aactggacct
    21061 gaaaaagctg atccagccag cgtggtcttt gatggtatgg aacgtgccgt ggctgaaggt
    21121 atcgatattc tcatgattga tactgctggt cgtctgcaaa ataaggataa ccttatggct
    21181 gagttggaaa agattggtcg tattatcaaa cgtgttgtgc cagaagcacc acatgaaacc
    21241 ttcttggcac ttgatgcatc aacaggtcaa aatgccctag tacaggccaa agaattttcg
    21301 aaaatcacac ctttaacggg aattgttttg actaagattg atggaactgc tcgaggaggt
    21361 gtggttctag ccattcgtga agaactcaat attcctgtaa aattgattgg ttttggtgaa
    21421 aaaatcgatg atattggaga gtttaactca gaaaacttta tgaaaggtct cttggaaggt
    21481 ttaatctaat cagaagcaaa aatcctgcaa ggcataaact tgcaggaaat ttttttattc
    21541 taagcgacca tcttgacgat aggtgatatc tggttgccaa gtccatttgg caccgaattt
    21601 ttcaagtagg tcaaagctgg cttgaggtcc catgcttcca gctttatagt catgaagtgg
    21661 ggcaccattt tcagcccaga gcttttcaat acggtcaatc aacttccatg acgcacaaac
    21721 ttcatcccag tggctaaagt tagttgagtt gttatttagg acatcataaa tcaatttttc
    21781 gtatggttct ggagaagcac cagttgcagt cgcatctgta cggtaatcaa gtgagttagg
    21841 agccaagtta aattcttctc ctacttgctt cccatttagg ctaagagaga agccttctgt
    21901 tggttgaata tagatggtca aaatatttgg agcaagtggt tctccaaaga tagaatccat
    21961 ttgtttaaag acgatgttga catgagttcc tttttcagtc agtcgtttac ctgtacggaa
    22021 aaagaaagga acaccacgga atcgatcgct gtctacaaag aaggcaccag atgtaaaggt
    22081 ttcagttgtt gattctggat tcacatttgg ctcgctacga taagagatgt atttcatgcc
    22141 atcaatctta ccagagcggt attgcccacg gataaagtgt tctttgagtt cttcatcagt
    22201 tggatgatag aggtttttaa agaccttaat cttttcagca cgaatctcgt cttttgtgaa
    22261 gcttgctggt ttgtccatgg cgaggagcga aagaagttgt agagtgtggt tttggaccat
    22321 gtcacggagg gcaccggatt ggtcatagta gccaccacgt tcttctacac ccaagcgctc
    22381 cgcaaaggta atttgaacat tgtcgataaa atccttgttc caaacgtttt caaaaatcaa
    22441 gtttgcaaag cgaactgcaa agatgctttg gatcatttcc ttaccaagat aatggtcgat
    22501 acggaaaatt tgttcttcgt caaatgttgc taggagttcg tcattcaact tgtttgcagt
    22561 tgcgtaatct gtaccaaatg gtttttcaac gatcaagcgc tcaaaacctt tgccatcgac
    22621 aatgttttca gatttgaggt gtttggcaat ggttccaaag aactgaggtg ccatagacaa
    22681 gaagaagagc ttattgtgtt cagcttggta tttttcatta agctcagctt gtaattgacg
    22741 caaagcaata taatgttccg aatcattgac atcatggctt tgatagtaga agtggctagc
    22801 aaattcttgg gcttgctcgg tactatctgc caaatcaagg atggactcga caactacaga
    22861 ttcaaaatat tccttactcc aaggtctacg ggcagttcca ataacggcaa agtgcttgga
    22921 aagattgccg gattgatata gtctaaaaag ggaagggtag agcttgcgtt tagccaggtc
    22981 tccactcgca ccgaaaattg taacaataac cttagatgac atctagctac ctaatttcta
    23041 tttttattcc tagtcttgct aggtatgagg aaacctcatt tcataaactt aattctaaca
    23101 taattttccg aaaaatcaaa aattccaata cgaggtagaa aaactgcaag gaaatcctta
    23161 cagtttgagt ttagacgttt aagaccttat ctaagaactc tttcagacga gggtgttgtg
    23221 ggttatcaaa gatttggtca ggtgttccgt cttcaaggaa ctcgccatct gcagtaaaga
    23281 taacgcggtt ggcaacctga cgagcaaatc ccatctcatg ggttacgata atcatggtca
    23341 taccttgctc agccaattcc ttcataacgt taagtacgtc tccaaccatc tcagggtcaa
    23401 gggcagaagt tggttcatcg aagagcatga tgtctggatt cattgctagg ccacgagcga
    23461 tggccacacg ttgtttttga ccacctgata ggctatctgg attggcatta gctttatctg
    23521 ctagtccaac cttttcaagc aactccattc ccaattcctc agcttcttcc ttagtcatca
    23581 acttgtgctc aataggagca aaggtgatgt tgtccaatac agacatatga gggaagaggt
    23641 tgaagtgttg gaataccatg ccgatatttt cacggacgtg gtcaacattg gttgtttttt
    23701 cagttaaatc atagccgttc acagtgatgt gaccgctagt gacttcttct aaaagattga
    23761 ggctacggag gaaagttgac ttaccagaac ctgaaggacc gatgatacaa acaacatctc
    23821 cttcatagaa cttagtcgta attcctttta ggacttcatt ttttccatag tgcttgtgta
    23881 aatcatttac atcaattttt aattttgcca ttaacgaatc ctcttttcta agcgtttcgc
    23941 tagtctagtc aaaagtgtga taattacaag atagaagata gcaaggattg catacatctt
    24001 gaaactttgg tagttacgag caatgataat cttaccagtt tggaagagtt caaccaaacc
    24061 gatagcagat acgatagttg tatctttaag agcgataacg aattggttga caaagtttgg
    24121 caacatcaat ttagttgctt gtggcaagat aatcttacgc atggtttttc cataagagat
    24181 acccaagctt cggctggctt ccatttggcc aactggaacg gcctgaatac caccacgaac
    24241 gatttcagcg atataagcag ccgcattgag tgagagggca attgttccag ctacaaagtc
    24301 gttaattggg ctttgttggc ctgtgataga ctcgatgaag tttggaattc cccagaagat
    24361 gaaggctgca agaatcatca atggaatacc acgaataacg tcaacgaaaa tctcagagat
    24421 gacgcgaaga gatttgtatg ggctaacgct aaacataccg aagataattc cgatgacaat
    24481 ggcaatagca aatgagataa gagctagagc aagagtgata ccaagaccgc taaggagttg
    24541 tttgtagttg ttttgaagca agccccagag cgttgtttcg tcaacagtac ttgttgaagc
    24601 agttgaagat tcgctagcta ggtatttgtc aagaatcttt tggaattcac cgtttgcttt
    24661 aaggtttgca agtccgttgt tgaacatttc aatcagttct ggatttgctc cttttttaac
    24721 ggcaaaggct gtttcaccga ttggagttcc agagattgga gttttcaatt tttgaccttg
    24781 gctgatagaa tatttgagaa caggttcatc atccataacg gcatcaatgg caccagtgtt
    24841 taaactgtca tacattgaag aaccatcagc aaaggttttg attttgtaac cgtatttgct
    24901 ttgattttct gttaggaagg tttgagaagc agttccgttt ttaacaccga ctgtctttcc
    24961 ttttagatct tcataagaag caatattgct tgattctttg acaccaagaa tggtattagc
    25021 agtgtagtat gattctgaga agtcaaaagt tgccttacga gcatctgtga cagacatacc
    25081 agcaatgata ccatcggctt gaccagcttg gacagcactg atagcagcat caaaaccagg
    25141 gttggtgatt tcaatttcaa aaccttggtc tttagcgatt gccttaatca attccatatc
    25201 aataccagtg tattggttgc ttgaattttg gaaaacaaaa ggtgcaaaag aagaatcgct
    25261 ggcaatgata tatttagcct taacaggaat agcttttaat cctgcgagag tagttgtagt
    25321 tggcactgct gaagatgatg aagcagtcca tttcttgata attttatcaa gactaccatc
    25381 ttttttcatt tcagccaagg cttggttaaa ttcagtaacc aggtgctcgt atttacttcc
    25441 ttttttcaca ccgaaagcaa aacttcctac agcttcacca tccatttcaa tatggaggtc
    25501 ttgaccttgg ttaatggcat attcgataac aggtttgtca tccatcatgg catcgatggc
    25561 accagcactc aagctgttgt tcattaaatc cccagtgtca aatgttttaa tagtaaagcc
    25621 gtatttatct ttgattgttt caaggaaacg ttgagcggca gttccgtttt taacaccaac
    25681 ggttttgcca gttaattggt cgtacttgct aattttgtgt gactttgtag tagcaatgac
    25741 aacttttgta tcatagtaag tatcagacat ggtgaagact ttttcacgtt ctttagtctt
    25801 tgtcatccct gccatgatag cgtcggcttg cccagcttga accgcattga ctgctgcgtc
    25861 aaatccagga taggacatct gaatgttcca gcctttaatc tcagcgactt tgttaataat
    25921 gtcaacatca attcctttat aagtttgatc tgaatcttta aactcaaaag gtgcataggc
    25981 ggtatcagaa acaatcttaa tcgtttctgc tttggcaata cctaatgaga aaattgggaa
    26041 taaaattagc aaaaatgcta gaaatttttt cttcattttt agtctccttt tccgaatatt
    26101 ctctcattat atcagaaaaa gtaaaaaaag gcaaattttt atatttttcg tgtcagaaaa
    26161 tataacattg gtttttcctt ttctttcttg aattgctatt aaaaagtgct ataataatag
    26221 tatatataga agaaaagagg acggggatat gaagaacaaa agaatattta aagacttcca
    26281 agcttcaaaa atgagtttaa acatttacac aagccccttg ttagcctttg tttttgtctt
    26341 cataggagag tttgtggctt atactttata tggtattagt ttgttagctc tcatcggact
    26401 tgctagaaat tttggagagg ctggtcaaaa tcttgcaagc tacttgcaga ccttgcatca
    26461 gagcttgacg gataaaacaa gtgactttcg tttaatttta ggattactgg cctttggttt
    26521 tattcttaac actgtgttca gatggacaag aaaagttgag aaaagaccta ttcgaacctt
    26581 gggattttat agagagaatt tcctcagcaa tcttctgaaa ggatttagtc taggcctggc
    26641 actttttctt ctgaccttgt taggtttggt ggtcttaggt caatatcgtt tggaatccat
    26701 tcacttgaat ccttattctc ttgcctttgt cgtctttact atcccatttt ggattttaca
    26761 ggggacagca gaagaagtgg tggcccgtgc ttggctactt cctcaattgg cctcaagaac
    26821 caatctaaaa ctagctattc ttatatctag cctgttcttt accctgcttc atatgggcaa
    26881 ttctggcctc acccctctat ctctagtaaa tctcttttta ttcggagttg ccatggctct
    26941 ttaccttctc aaaactgata cagtttgggg tgttgcaggt attcatggtg cttggaattt
    27001 tgctcagggt aatctctttg ggattttagt tagtggtcaa ccgtcaggaa cgtctctgat
    27061 gaccttttta ccacaaggca atcaagattg gctatcaggt ggttcttttg gcatagaagg
    27121 ttccattatg acaagtctgg tattactact gctgattgtc tatcttgcta ataaattaaa
    27181 gaaagaaaat gaaaggatgt gacttcggtc cgtccttttc ttcgtgaaaa tactataagt
    27241 atgctaaaat aggaatagca catggagaga ggattcttat gatcaatcac attacagata
    27301 atcaatttaa actagtatca aaatatcaac catcaggaga tcaaccccaa gctatcgagc
    27361 agttggtgga taacattgag gggggagaaa aagctcagat tctgatgggg gcgactggaa
    27421 cagggaagac ctatactatg agtcaggtca tttctaaagt caataaacca actctggtta
    27481 ttgcccacaa taaaactctg gctggtcagc tctatgggga gtttaaggaa tttttccctg
    27541 aaaatgcagt tgagtatttc gtatcctact atgattatta ccagccagag gcctatgtcc
    27601 cttctagcga tacctatatt gagaaggata gttctgtcaa tgacgagatt gacaagcttc
    27661 gccactcagc tacctcagca cttttggagc gtaatgatgt tattgtcgtg gcctcagtct
    27721 cttgtatcta tggtttgggt tcgcccaagg aatacgctga tagtgtcgtt agtctccgtc
    27781 ctggtctaga gatttctcgt gataaactct tgaatgactt ggtcgatatt cagtttgaac
    27841 gcaatgatat tgatttccaa cgcggaagat ttcgcgttcg tggggatgtg gtagagattt
    27901 tcccagcttc ccgagatgaa catgcctttc gagtagaatt ttttggagac gaaattgacc
    27961 gtattcgtga agttgaggct ctgacaggtc aggtgttggg agaagtggat catttagcga
    28021 ttttcccagc gacacacttt gtgaccaatg acgaccatat ggaagttgcc attgcaaaga
    28081 ttcaggccga gttggaagaa caattagctg tctttgaaaa ggaaggtaaa ctgcttgaag
    28141 cccagcgttt gaaacagcgg acagagtatg atatcgaaat gttgcgtgag atgggctata
    28201 ccaatggggt tgagaactat tcacgacaca tggatggacg gagcgaagga gagcctcctt
    28261 atacgctctt agatttcttc ccagatgatt tcttaattat gattgacgag agtcacatga
    28321 ccatggggca aatcaagggc atgtacaatg gagaccgttc gcgtaaagaa atgctggtta
    28381 attatggctt ccgattgccg tctgctttgg acaatcgtcc tctccgtcgg gaggagtttg
    28441 agagtcacgt tcatcagatt gtttacgttt cagcgacacc tggtgactat gaaaatgaac
    28501 agactgagac agtgattgag caaatcattc gtccaacggg actcttggat cctgaagtgg
    28561 aagtccgtcc gactatggga cagattgatg acctcttggg tgaaatcaat gcccgcattg
    28621 aaaaaaatga gcgtaccttt atcacaactt tgaccaagaa aatggcagag gacttgaccg
    28681 actacttcaa ggaaatgggt atcaaggtca agtacatgca ctcggatatc aagaccttgg
    28741 aacggacgga gattatccgt gacctgcgct tgggtgtctt tgatgtcttg gtcggaatta
    28801 acctgctccg tgaaggaatt gacgttcctg aagtgagcct cgtagctatt ctcgatgctg
    28861 ataaggaagg tttccttcgc aacgaacgtg gactcatcca gactattgga cgtgctgctc
    28921 gtaacagtga aggccatgtc atcatgtatg cagacacggt tacccagtct atgcaacgtg
    28981 ctatcgatga aactgcccgc cgtcggaaaa ttcagatgac ctataatgaa gagcatgata
    29041 tcgtaccaca gacaatcaag aaagaaatcc gtgacctgat tgccgtgacc aaggcagttg
    29101 ctaaggaaga ggacaaggaa gtcgacatca atagcctcaa caaacaagag cgcaaggaac
    29161 tcgtcaagaa actagaaaaa caaatgcaag aagccgtcga agtgcttgac tttgaactgg
    29221 cggctcagat tcgtgatatg atgttggaag tcaaggtctt ggattagggg aatagtatga
    29281 tttatttaag aaagttaaag agagaagatt tgatgtcttt atgggaaatg gcttattcac
    29341 aacttaatcc agtttggaaa cagtatgatg ctccctatta tgatgattat cagtattttt
    29401 caaattttaa agaattcgaa ctacaaaaat cagaatccat tttaagcaac tcaaatcgcc
    29461 ttggtatttt tgttgatgat aaactagttg ggactgtttc gcgttattgg gtatgtaaac
    29521 aaacaagatg gatggaattg ggaattggta tttatgataa aaaattctgg aacactggta
    29581 ttgggaaagt tgctatgttg cagtggatag ataggacgtt tcaggattac ttggagttgg
    29641 agcatctggg tttgacaact tggtcaggga atcttggtat gatgaaactt gctgaaaaat
    29701 taagaatgaa aaaagaagct catattccaa aagttcgtta ttatcaaggt aaatattttg
    29761 atagtattaa atatggtatt ttgagagaag actgggagaa aataaatgac ggttattatc
    29821 aaatcaatgg aaactcctga agagatagaa ggtaaatccc ttgttcactg gcaaacgtgg
    29881 agagaggctt atgatgacct tttgcctgca gaatttcagg agacaatgac attagaaaga
    29941 tgtcgattct ttagtcaaaa gtatccagaa aatacattga ttgcgatagg tgatctgaag
    30001 gtagttggtt ttatcagtta tggtaacttt cgtgatgaga ctattcaagc tggtgaaatt
    30061 attgctttat atgttttaaa agactattac ggaaaaggca tagcacaaaa gttaatgaaa
    30121 gcagctttga ctgctctgaa tcatttttct gaaattttct tatgggtatt gaaagataac
    30181 aagcgagcca ttgctttcta tcaaaaaatg ggttttactt ttgatggaca agaaaaaata
    30241 cttgaacttg gaaagcctat aaaggaaaaa cgaatggtgt tctattctaa ataattttaa
    30301 aaaagtaaaa gataatatct ctggtagtaa gtctgaaaat ttaataaatt agaaagcgag
    30361 taaatttatg tcccgttccc aattaacaat tttaacaaat atctgtctga ttgaagacct
    30421 cgaaactcag cgcgtggtga tgcagtatcg cgcccctgaa aacaatcgct ggtctggtta
    30481 tgcctttcct ggaggtcatg tagaaaatga tgaggctttt gcggagtctg tcattcgtga
    30541 aatctacgaa gaaacagggt tgactatcca aaatcctcaa cttgtcggca ttaaaaattg
    30601 gccactagat acaggtgggc gctatattgt catttgttat aaggcgactg agttctctgg
    30661 tacccttcaa tcttcagaag agggagaagt ttcttgggtg caaaaagacc agattccaaa
    30721 cttaaatctg gcctatgata tgctaccatt gatggaaatg atggaagctc ccgacaagtc
    30781 agagtttttc tatcctcgcc gtacagaaga cgattgggaa aagaaaatct tctagtcttt
    30841 tactaaataa cctagctgat ccaaggcctc ctcgatatag tggaggtctt gttgtgtttc
    30901 ggcttcgact aggtggtaat ggatgccatc tgtcaattca gatagaggtc taaagtcaga
    30961 atgctcgact tgttctagaa aatgttgcac atctctgcga caagacagtt taagcaaggt
    31021 ttcaatttct ccataaacgg gatggtcaat caaggtattc tggacatgcc caccattatc
    31081 gacaatagca aggagttcct gaccgatttc ttcaacttca tgtttcactt tgaaaagttt
    31141 gtgaacataa ggattggtat caatttgctt atagatgtag ccacgattgg tggataggat
    31201 aggagcgcca tcggctctca aaattgcaat gtcctgcaca atgacctgac gtgtgacatg
    31261 aaaatgttcc gccaaacttt ggccattaag ggctttagga gcttctttca agagttggag
    31321 cagggcttgt ttgcgatctt ttgtcatagc ttttcctttt aacggcgttt tcgaagcact
    31381 ttatagacag ctagtgctaa tgtatagtct accatactat ggataattgt gccaaatcca
    31441 actagtacaa atagaacata gaacatattt tctacattgg tacccgaagt tgcgtaaaaa
    31501 aggacacagg ctaatacttc agcaagggca tgaacaacag ccaaaacaaa attgaaaacc
    31561 caggaagatt ttggtttatc tagggtatcg gggaattttt gtaggtaaag agctcctaaa
    31621 gcaccaaaag atatatggga aaaagcccga aaaacgataa ccatgggata gccagccatc
    31681 aaaaatccaa aactagaggc taggatgaca aaaactgcca tcaagggcga caagaacatg
    31741 gctataaaaa tagcgatgtg gctccccaaa gtataggaag caggtggaat gacaatcttg
    31801 aaaggcataa caattggaat caaaatcgca atagccgtta aaagggctgt cattgtcata
    31861 aattgtgtct ttttccgtgt attcacaaga atctcctttt taactgcata tacactagta
    31921 tggtacaata aaccagacaa taaagcaaga atttacttgg gtttatagat cattttttag
    31981 ttaaaagtta tagtagatgg aaactagaat agtccacctc tacttctaaa acattgttag
    32041 cgatttgact gtcctgatcg atttgtcctg ttcttatttc gttttactat agtaaagatt
    32101 tcattaaaaa gaaactgtat agagcaaaat ctccaccttc aggtttgaaa gcggagattg
    32161 tttttatttt ttcaaggttt gtagtcgtgg aacaatggct agggttagaa ttgcagaaat
    32221 gaccaattca gcaattgaat ttgttgagat aacggttgcc agaagaagtt ggatatttcc
    32281 attataaaca tttccaaata ggaagaagat tcctccaagg acaaagatag tatttgtcaa
    32341 ggaaccaagg gctccagcta aaatcagacc agtcttgttt ttcatcagtt tatagactaa
    32401 gtaaggagtt aaaccaatca aaatacgtgg gacgatggca atgatagctg agtagatgtt
    32461 tccgtttggt acgaagggag agaagaggta gcttgtcggt agaatcgtaa tcgtgttaac
    32521 cgtcaagcta agtaatccca tcaaaaatcc aagtgtaacc ccaactcgtg gaccataaat
    32581 aatgctggca ataatgacag gaatatgaac aatggtcggt ttgattggaa atggaaaaag
    32641 gttaaagata agtgagctca gaaagtgtat cacgagcatg gtagcaaaaa agatagcaat
    32701 gggtgcaata ttagagcgtt ttttcatcga tagtttcctt tattctttct aaaataattg
    32761 tgaggtcagc taaagctcct cgtccgtggt ctccacaagc tagtagggat tccttaggag
    32821 caatcagctg atagccgtag gtttctaatg ttttcagatt attctgagtt actggatggt
    32881 catacatttt tgtattcata gcaggagcta ttagtttggg aatatgactt ggtagggcta
    32941 gagctgtact ggttaccatg ttgtccgcaa atccgtgagc tagttttgca atagtgttag
    33001 cagttgcagg taccacgata aataaatctg ctttttttcc aagttcgata tgattgacct
    33061 gatcaggata gggttccttc atgacatcca agtggacagg attctgtgag agtacctgta
    33121 gtgtcaaagg ttggataaac tctgtagcag cctgagtcat taagacagtg acttgatggc
    33181 cttgtttttt tagagaactg actaaatctg ccgacttata agaggcgatt gaacccgtta
    33241 cagccaagag aatgtttgcc atagttttcc tttctaagaa tgataggctt gaattttttc
    33301 aaggaggagt tctgcaattt cttctttagt ctggactgtt tgaagctgat ttttctcaac
    33361 aaatatagct cggtgctgat ctgctgaaat ttgagtcagg tcattcgcga tgattaaatc
    33421 tgcttgattc ttgataagac tttttcgtgc aatgtcaacc agatgatctt cggtaacatc
    33481 aaccagcagt ttgaaaccaa tcagatgaat agtgggattc cattccttga ctagggatat
    33541 gattttgggt gtctttttaa ggaacaaaac ctgaacctca tcagttgaag aaatcttggc
    33601 ctgatgattc tgcttgctta aaaattcttt tagattggag ctagcctgaa cttcctcaag
    33661 ccctgtcata taaacaggag tgtagtcaga aacagccatt gagtggatca agacctgata
    33721 atcctgaaca cgttcttgca tttctattag aaggtccttg gtattggtaa tttctcgaat
    33781 acttaggtta ggatgaggct ctggcttcag agctcgtttt gtcgtaatta aacaaacttc
    33841 atgccctgca gaaagcaaag tctctgtgat aattttcccc aagtgacctg tagaatggtt
    33901 agtgatagag cggacgctat cgatagcttc actggtaccg cccgatgtaa ctaaaatttt
    33961 catagcacta ttgtacacaa aaataagcgg taagtaaaat gaaagtgata aatttttggt
    34021 aaaaacgaag atttactata gtttcaaact ataaagccat ataaaattta agaaaggact
    34081 ctaaaagcct taaaaacaga gatatttacg aacgtttaga gagttttagg atgttaaaaa
    34141 tcttgttttg tgatataatg aattatcata taagaggtta gagaaatttt gaatgaaaac
    34201 agatattgaa atcgcacaga gtattgagtt gaagccaatt gttgatgttg tagagaaact
    34261 tggtatttct tatgacgatt tggagttgta cggaaagtac aaggctaaac tcagctttga
    34321 taaaattcgt gcagttgaga gcaatccagt cggtaaattg atcttggtta ctgccatcaa
    34381 cccaacacct gcaggtgaag gaaagtcgac gcttaccatt ggtcttgcgg atgccttgaa
    34441 caagattggc aagaaaacca tgattgctat ccgcgaaccg tctcttggtc cagtcatggg
    34501 gatcaagggt ggtgctgctg gtggtgggta tgcacaagtt ctgccaatgg aagacatcaa
    34561 cctccacttt actggagata tgcatgctat tacaactgcc aacaatgccc tttctgcctt
    34621 gattgacaac cacttgcacc aagggaatga gctggaaatt gatcaacgtc gtatcctctg
    34681 gaaacgcgtt gtggacttga acgaccgtgc gctccgccat gtgactgttg gtcttggtgg
    34741 tcctctaaac ggtattccac gtgaggatgg ttttgatatt acagttgctt cagaaatcat
    34801 ggcaattctt tgcttggcaa cggacatcga ggacttgaaa cgtcgtttgg cgaatatcgt
    34861 tattggttat cgctatgacc gtacgcctgt ttctgtaggt gatttgcagg ttgagggtgc
    34921 cttggctttg attttgaagg atgctattaa gccaaacttg gttcagacaa tttacggtac
    34981 acctgccttt gtacacggtg gtccatttgc caatatcgct catggctgta actctgtttt
    35041 ggcgacgaca acagcccttc acttggctga ttacactgtt actgaagctg gttttggtgc
    35101 ggaccttggt gctgagaaat tccttgatat caagacacca aacttgccaa catctccaga
    35161 tgcagttgtc attgtcgcaa cccttcgtgc ccttaagatg aatggtggtg tggctaaaga
    35221 cgctctgact gaagaaaatg tagaggcagt tcgtgcaggt tttgctaact tgaaacgcca
    35281 cgttgaaaat atccgtaagt tcggtattcc agcagttgta gctattaacg aatttgtatc
    35341 tgatacagaa gctgaaattg cagtcttaaa agaactctgt gcctcaatcg atgtaccagt
    35401 tgaattggct agtgtctggg ctgatggagc agaaggtgga gtagcacttg ccgaaacagt
    35461 tgttaagaca attgctgaaa atccagctaa ctataaacgt ttgtacgata atgccctttc
    35521 tgtccaagaa aagattgaaa aaattgtcac tgaaatctat cgtggtagca aagtgaactt
    35581 tgagaagaaa gctcaaacac aaattgctca aatcgttcaa aacggttggg acaaattgcc
    35641 aatctgtatg gctaaaactc aatacagttt ctcagacaat ccaaatgcgc ttggagcacc
    35701 agaaaacttt gaaattacca ttcgtgaatt ggtaccaaaa ttaggtgcag gcttcatcgt
    35761 tgccttaact ggtgatgtta tgaccatgcc aggtcttcca aaacgtccag cagctctcaa
    35821 catggatgtt gaaagcgatg gaactgtact aggcttgttc tagtggattg aaattgactt
    35881 taagtgagtt tggaaataat atccaagctc attttttctc agactgcaag cagttcatca
    35941 ataagattta tactcttcga aaatctcttc aaac
//
LOCUS       DAVBCN010000011        35189 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVBCN010000011 DAVBCN010000000
VERSION     DAVBCN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35189
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.011"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(<1..180)
                     /locus_tag="VQ497_000527"
     CDS             complement(<1..180)
                     /locus_tag="VQ497_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET2516880.1"
                     /translation="MNYEASKQLTDARFKRLVGVQRTTFEEILAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQ"
     gene            175..429
                     /locus_tag="VQ497_000528"
     CDS             175..429
                     /locus_tag="VQ497_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET2516881.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIGAEAIRRNCERGAIFSGKIKHHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            773..2107
                     /gene="trmFO"
                     /locus_tag="VQ497_000529"
     CDS             773..2107
                     /gene="trmFO"
                     /locus_tag="VQ497_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HET2516882.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRIFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEAIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            2191..2928
                     /locus_tag="VQ497_000530"
     CDS             2191..2928
                     /locus_tag="VQ497_000530"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET2516883.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            2937..3494
                     /gene="frr"
                     /locus_tag="VQ497_000531"
     CDS             2937..3494
                     /gene="frr"
                     /locus_tag="VQ497_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET2516884.1"
                     /translation="MANAIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRIHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVV
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            3554..4408
                     /gene="cvfB"
                     /locus_tag="VQ497_000532"
     CDS             3554..4408
                     /gene="cvfB"
                     /locus_tag="VQ497_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HET2516885.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTSLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4417..4632
                     /locus_tag="VQ497_000533"
     CDS             4417..4632
                     /locus_tag="VQ497_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HET2516886.1"
                     /translation="MRKSFYTWLMTERNPKSNNPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            4718..5686
                     /locus_tag="VQ497_000534"
     CDS             4718..5686
                     /locus_tag="VQ497_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET2516887.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMALVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            5866..6366
                     /locus_tag="VQ497_000535"
     CDS             5866..6366
                     /locus_tag="VQ497_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2516888.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            6369..6629
                     /locus_tag="VQ497_000536"
                     /pseudo
     CDS             6369..6629
                     /locus_tag="VQ497_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(6996..8107)
                     /gene="ald"
                     /locus_tag="VQ497_000537"
                     /pseudo
     CDS             complement(6996..8107)
                     /gene="ald"
                     /locus_tag="VQ497_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253293.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
     gene            8284..8853
                     /locus_tag="VQ497_000538"
     CDS             8284..8853
                     /locus_tag="VQ497_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2516889.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            8921..9571
                     /locus_tag="VQ497_000539"
     CDS             8921..9571
                     /locus_tag="VQ497_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET2516890.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTSVKETNLQ
                     AEVAAVSKDSSTGKEVNKEEKEEPVEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            9555..11795
                     /locus_tag="VQ497_000540"
     CDS             9555..11795
                     /locus_tag="VQ497_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HET2516891.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSVSYFALLGFVFLLVCL
                     FIQFPWKSAGKVLVICGVFGFWFLFQTWQQSQVSQNLVDSVERVRILPDTIKVNGDSL
                     SFRGKSDGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFDYQGY
                     LKTQGIYQTLNIKRIQSLQKVGSWDIGENLSSLRRKAVVWIKMHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMDGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     VFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVASRPLVFGQPNEWLLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIKKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEMTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMIVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            11975..12163
                     /locus_tag="VQ497_000541"
     CDS             11975..12163
                     /locus_tag="VQ497_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516892.1"
                     /translation="MTVTKSYKYDWNTVLEYSTNYHDHQYAWIPEWSRYYSYSEYKVG
                     GGWNYARYEVINYYTGGY"
     gene            12195..12782
                     /locus_tag="VQ497_000542"
     CDS             12195..12782
                     /locus_tag="VQ497_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2516893.1"
                     /translation="MLNLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            12786..13961
                     /locus_tag="VQ497_000543"
     CDS             12786..13961
                     /locus_tag="VQ497_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516894.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWKDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLPKQIDNVDLNWSMGMVLLPCLIIFLLLGILFIERWGSSQKKRIF"
     misc_feature    14135..14266
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14282..14812
                     /gene="infC"
                     /locus_tag="VQ497_000544"
     CDS             14282..14812
                     /gene="infC"
                     /locus_tag="VQ497_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET2516895.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            14845..15045
                     /gene="rpmI"
                     /locus_tag="VQ497_000545"
     CDS             14845..15045
                     /gene="rpmI"
                     /locus_tag="VQ497_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET2516896.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            15097..15456
                     /gene="rplT"
                     /locus_tag="VQ497_000546"
     CDS             15097..15456
                     /gene="rplT"
                     /locus_tag="VQ497_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET2516897.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            15514..15894
                     /locus_tag="VQ497_000547"
     CDS             15514..15894
                     /locus_tag="VQ497_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2516898.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            16144..16944
                     /locus_tag="VQ497_000548"
     CDS             16144..16944
                     /locus_tag="VQ497_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HET2516899.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVNQRVCEDGPVFRTGTVVL"
     gene            16907..17893
                     /locus_tag="VQ497_000549"
     CDS             16907..17893
                     /locus_tag="VQ497_000549"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HET2516900.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTNLPIIGMGGVDSAEAALEM
                     YLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            17935..19704
                     /locus_tag="VQ497_000550"
     CDS             17935..19704
                     /locus_tag="VQ497_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HET2516901.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMAKNETVDGYQLGSDGKWLGGKATNENAAYYQVVPVTANVYDSDG
                     EKLSYISQGSVVWLDKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYESDG
                     HRFYHYVAQNASIPVASHLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATNYSA
                     EELDKVFSLLNINNSLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKDKN
                     NFFGITAYDTTPYLSAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEYAS
                     DPYWGEKIASVMMKINEKLGGKD"
     gene            complement(19765..21420)
                     /gene="pavA"
                     /locus_tag="VQ497_000551"
     CDS             complement(19765..21420)
                     /gene="pavA"
                     /locus_tag="VQ497_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HET2516902.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTKSLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPLANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            21525..22022
                     /gene="ybeY"
                     /locus_tag="VQ497_000552"
     CDS             21525..22022
                     /gene="ybeY"
                     /locus_tag="VQ497_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET2516903.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLGYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            22003..22398
                     /locus_tag="VQ497_000553"
     CDS             22003..22398
                     /locus_tag="VQ497_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HET2516904.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFGFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            22415..23314
                     /gene="era"
                     /locus_tag="VQ497_000554"
     CDS             22415..23314
                     /gene="era"
                     /locus_tag="VQ497_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET2516905.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPDRFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            23363..24187
                     /gene="mutM"
                     /locus_tag="VQ497_000555"
     CDS             23363..24187
                     /gene="mutM"
                     /locus_tag="VQ497_000555"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HET2516906.1"
                     /translation="MPELPEVETVCRCLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARSSQTLTAEEATAIHDQTIAVLSQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQGCVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            24187..24792
                     /locus_tag="VQ497_000556"
     CDS             24187..24792
                     /locus_tag="VQ497_000556"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET2516907.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFWQEIILENGELNRPLLASLIFSNPDEREWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            24779..25978
                     /locus_tag="VQ497_000557"
     CDS             24779..25978
                     /locus_tag="VQ497_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HET2516908.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            25978..26127
                     /gene="rpmG"
                     /locus_tag="VQ497_000558"
     CDS             25978..26127
                     /gene="rpmG"
                     /locus_tag="VQ497_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2516909.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            26167..26400
                     /gene="secG"
                     /locus_tag="VQ497_000559"
     CDS             26167..26400
                     /gene="secG"
                     /locus_tag="VQ497_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET2516910.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            26502..28856
                     /gene="rnr"
                     /locus_tag="VQ497_000560"
     CDS             26502..28856
                     /gene="rnr"
                     /locus_tag="VQ497_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HET2516911.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVSSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSHSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            28819..29286
                     /gene="smpB"
                     /locus_tag="VQ497_000561"
     CDS             28819..29286
                     /gene="smpB"
                     /locus_tag="VQ497_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET2516912.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NIKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            29302..30162
                     /gene="tehB"
                     /locus_tag="VQ497_000562"
     CDS             29302..30162
                     /gene="tehB"
                     /locus_tag="VQ497_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HET2516913.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEDVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            30235..31188
                     /gene="coiA"
                     /locus_tag="VQ497_000563"
     CDS             30235..31188
                     /gene="coiA"
                     /locus_tag="VQ497_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HET2516914.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKKQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            31207..33009
                     /gene="pepF"
                     /locus_tag="VQ497_000564"
     CDS             31207..33009
                     /gene="pepF"
                     /locus_tag="VQ497_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET2516915.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEVLKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTFYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGIYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            33011..33724
                     /locus_tag="VQ497_000565"
     CDS             33011..33724
                     /locus_tag="VQ497_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET2516916.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELENFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            33791..34732
                     /gene="prsA"
                     /locus_tag="VQ497_000566"
     CDS             33791..34732
                     /gene="prsA"
                     /locus_tag="VQ497_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HET2516917.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(34849..>35189)
                     /locus_tag="VQ497_000567"
     CDS             complement(34849..>35189)
                     /locus_tag="VQ497_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2516918.1"
                     /translation="LEQAFKEKHYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRK
                     GNSQDNGRMESFFGILKSEMFYGYEKTFKSLNQLEQAIIDYIDYYNNKRIKVKLKGLS
                     PVQYRTKSFR"
BASE COUNT        10584 a         6536 c         7936 g        10133 t
ORIGIN      
        1 ttgaagagtg gccataagaa ggtcttctag gcttaattta ggttttcgtc caccttttgc
       61 gtgtttaagt tgataagctg tttttaatac agctaatatc tcttcaaaag tcgtgcgctg
      121 aacaccaaca agacgcttaa atcgtgcatc agttagttgt ttacttgctt cataattcat
      181 agaactacta taccatattt tgtttcgcag gaagtctatt ggaaagtaag aaatattgga
      241 gctgaggcga ttagaagaaa ttgtgagcgt ggtgctattt tttcaggtaa aataaaacat
      301 cacgaagatt ctcagtttaa aggagaccac tatgttgaat gttatgctgt tttagataat
      361 acggttatag caagagatag aataacagtc cctatcgatc cgttatgtgg aaaagatttt
      421 atagagtagc atataattga ttcttaactg gaatactctc tatctcttta catcaagaaa
      481 atgactaaac agggaagttt gccttcttcc ctttttttgt tatactagta gaagaaaaaa
      541 atagaaagat ttgtgggagt gaaaaagtcc gggggacttt ttcagcttga gccaagaaat
      601 tcgagagcga ggaaagtccg ggggactttt tcagcctgag ccaagaaatt cgagagcgaa
      661 gtaagtccaa tggacttttt cagcctgagc caagaaactc gaaagctcgt aagtctgatt
      721 ggctttttca atgtgaatct taacttcaca ctcccaaaga ggtattagtg tcgtgtctca
      781 atcttatatc aatgttatcg gtgctggttt ggcaggttct gaagcagctt accaaatcgc
      841 agagcgtggt attccagtta aactatatga aatgcgtggt gtcaagtcga ctccccagca
      901 taaaacagac aattttgctg agttggtttg ttccaattct ttgcgtgggg atgctttgac
      961 aaatgcagtt ggtcttctca aggaagaaat gcgtcgcttg ggttctgtta tcttggaatc
     1021 tgctgaggct acacgtgttc ctgcaggtgg tgcccttgca gtggaccgtg atggtttctc
     1081 tcaaatggtg accgaaaaag ttgccaacca ccccttgatt gaagtggttc gtgatgaaat
     1141 tacagaattg ccgacagatg ttattacggt tatcgctact ggtcctttga caagtgatgc
     1201 cttggctgaa aagattcatg cccttaatga cggtgatggt ttttatttct acgatgcggc
     1261 agcgcctatt atcgatgtca acactatcga tatgagcaag gtctacctca aatcacgtta
     1321 tgataaggga gaagcggcct acctcaatgc ccctatgacc aagcaagaat ttatggattt
     1381 ccatgaagct ttggtcaatg cagaagaagc accgcttaat tcttttgaaa aagaaaagta
     1441 ctttgaaggc tgtatgccta tcgaagtcat ggccaaacgt ggcattaaaa ctatgcttta
     1501 tggccctatg aagccagtcg gtcttgagta cccagacgac tatacaggac ctcgtgatgg
     1561 agaatttaaa acaccttatg cggttgtgca acttcgtcag gacaatgcag ctggtagcct
     1621 ctacaatatt gttggtttcc agacccacct caaatgggga gaacaaaagc gtatcttcca
     1681 aatgattccg ggtcttgaaa atgcagagtt tgtccgttat ggtgtcatgc atcgcaattc
     1741 ttacatggat tcaccaaatc ttcttgagca gacttaccgt tctaagaaac aaccaaatct
     1801 cttctttgct ggtcagatga cgggggtgga aggctatgtt gagtcggcgg cttcaggctt
     1861 agttgcggga attaacgcag ctcgtctctt caaggaagaa agcgaggcta ttttccccga
     1921 gacgacagcg attggaagct tagctcatta cattacccat gccgacagca aacatttcca
     1981 accaatgaat gtcaattttg ggatcatcaa ggagttggaa ggcgagcgta tccgtgataa
     2041 gaaggctcgt tatgaaaaaa ttgcagagcg tgcccttgcc gacttagagg aatttttgac
     2101 tgtctaattt ttttgaaaga attgctcatg atactataaa aatcttagaa attgtgataa
     2161 aataggtagg atgaaagaag gagagtgaaa atggcgaatc ccaagtataa acgtatttta
     2221 atcaagttat caggtgaagc ccttgccggt gaacgtggcg tagggattga tatccaaaca
     2281 gttcaaacaa tcgcaaaaga gattcaagaa gttcatagct taggtatcga aattgccctt
     2341 gttattggtg gaggaaatct ctggcgtgga gaacctgcag cagaagcagg tatggaccgt
     2401 gttcaggcag attacactgg aatgcttggg actgttatga atgctcttgt gatggcagat
     2461 tcattgcaac aagttggggt tgatacgcgt gtacaaacag ctattgctat gcaacaagtg
     2521 gcagagcctt atgtccgtgg acgtgccctt cgtcaccttg aaaaaggccg tatcgttatc
     2581 tttggtgctg gaattggttc accatacttc tcgacagata caacagcggc ccttcgtgca
     2641 gctgaaatcg aagcggatgc catcctcatg gctaaaaatg gtgtcgatgg tgtttacaat
     2701 gccgatccta agaaagataa gacagctgtt aagtttgaag aattgaccca ccgtgacgtt
     2761 atcaataaag gtcttcgtat catggactca acagcttcaa ccctctcaat ggacaacgac
     2821 attgacttgg ttgtcttcaa catgaaccaa tcaggcaaca tcaaacgtgt cgtatttggt
     2881 gaaaatatcg gaacaacagt ttcaaataat atcgaagaaa aggaataaga aagaatatgg
     2941 ctaacgcaat tattgaaaaa gctaaagaga gaatgaccca gtctcaccaa tcacttgctc
     3001 gtgaatttgg tggtatccgt gctggtcgtg ccaatgcaag cttgcttgac cgtatacatg
     3061 tagaatacta tggagttgaa actcctctta accaaatcgc ttcaattacg attccagaag
     3121 cgcgtgtttt gttggtaaca ccatttgaca agtcttcatt gaaagacatc gaacgtgcct
     3181 tgaacgcttc tgatcttggt atcacaccag ctaatgacgg ttctgtgatt cgcttggttg
     3241 tcccagctct tacagaagag actcgtcgtg accttgctaa agaagtgaag aaggtcggcg
     3301 aaaatgctaa agtggctgtc cgcaatatcc gtcgcgatgc tatggacgaa gctaagaaac
     3361 aagaaaaagc aaaagaaatc actgaagacg aattgaagac tcttgaaaaa gacattcaaa
     3421 aagtaacaga cgatgctgtt aaacacatcg acgatatgac tgccaacaaa gagaaagaac
     3481 ttttggaagt ctaaaaataa acagaaaaac tcagttggca ttgctggctg agttttattc
     3541 gaaagaagga aatatgaata caaatcttgc aagttttatc gttggactga tcatcgatga
     3601 aaacgaccgt ttttactttg tgcaaaagga tggtcaaacc tatgctcttg ctaaggaaga
     3661 aggccaacat acagtagggg atacggtcaa aggttttgca tacacggata tgaagcaaaa
     3721 actccgcctg acaagcttag aagtgactgc cactcaggac caatttggtt ggggacgtgt
     3781 cacagaggtt cgtaaggact tgggtgtctt tgtggataca ggccttcctg acaaggaaat
     3841 cgttgtgtca ctcgatattc tccctgagct caaggaactc tggcctaaga agggcgacca
     3901 actctacatc cgtcttgaag tggataagaa agaccgtatc tggggtctct tggcttatca
     3961 agaagacttc caacgtcttg ctcgtcctgc ctacaacaac atgcagaacc aaaactggcc
     4021 agccattgtt taccgtctca agctatcagg aacttttgtt tacctaccag aaaataatat
     4081 gcttggtttt attcatccta gcgagcgtta cgcagagcca cgtttggggc aagtattaga
     4141 tgcgcgcgtt attggtttcc gtgaagtgga ccgcactttg aacctctccc tcaaaccacg
     4201 ctcctttgaa atgttggaaa acgatgctca gatgattttg acttatttgg aaagcaatgg
     4261 cggtttcatg accttaaatg acaagtcatc tccagacgac atcaaggcaa cctttggcat
     4321 ttctaaaggt cagttcaaga aagctttagg tggtcttatg aaggctggta aaatcaagca
     4381 ggaccagttt ggtacagagt tgatttaggg aggcttatga gaaaatcatt ttacacttgg
     4441 ctcatgaccg agcgcaatcc taaaagtaac aatcccaaag caattttggc agacctcgct
     4501 tttgaagagg cagcttttcc aaaacataca gatgattttg atgaggtcag tcgctttttg
     4561 gaggagcatg ccagtttctc ttttaaccta ggagattttg acagcatttg gcaggaatat
     4621 ctagaacact agcatttatt cattgggttt gggctagtaa tttctccatc cctctgctat
     4681 aataaaaaga aataaaagga ttagagaggt tctttatttg aaggaacatt caatagacat
     4741 tcaactgagt catccagatg acctgtttca tctttttggt tccaatgaac gccatcttcg
     4801 tttgatggaa gaagagcttg atgttgtgat tcatgctcgt acggagattg tccaggtttt
     4861 gggagaagag tctgcttgcg aggaagcccg tcaggttatt caagctctca tggccttggt
     4921 aaatcgtggg atgaccgttg gtacgccaga tgtagtgacg gctattagta tggtcaaaaa
     4981 tgatgaaatt gacaagtttg tcgcccttta cgaagaagaa atcatcaagg ataatactgg
     5041 gaagcctatc cgcgtcaaaa ccttaggtca aaaactttat gtggacagtg tcaaacagca
     5101 tgatgtgacc tttggaattg ggccagcagg tacagggaag accttccttg cagtgacctt
     5161 ggcagtgact gcccttaaac gtgggcaagt caagcgaatt atcctaactc gtccagcggt
     5221 ggaagcggga gagagtcttg gatttcttcc gggtgatctt aaggagaagg tggatcctta
     5281 ccttcgtcct gtttacgatg ccttgtatca aattcttggg aaagaccaaa cgactcgtct
     5341 catggagcgt gaaattatcg aaattgcgcc ccttgcctat atgcgtggcc ggaccttgga
     5401 tgatgccttt gtcattctcg atgaggcgca aaacacgacc atcatgcaga tgaagatgtt
     5461 cttgacgcgt ttaggctttc attctaagat gattgtcaat ggagatatca gtcagattga
     5521 cctgccacgt aatgtcaagt ccggtttgat tgatgctcaa gagaaactca agaacatcca
     5581 tcagattgac tttgttcatt tttcagccaa ggatgtggtt cgccatccag ttgtcgctca
     5641 gattatccga gcctatgaat attctactga agttgcacac gactgatttt gaggaagttc
     5701 gcctgcaaaa gaatatactt ttgagagagg aaaatccagt tgtataggct aaaggtttga
     5761 gacaacgatt aggcacgatg gaaagaactt ttatgtggct gatgacgatc agtgcatctt
     5821 cctgtgtcat aatcacaggg cacaagaaag taggaatttg aaaagatgat tgaccaacta
     5881 tctaagtatt acagttgtag gatactaact gaaaaggata ttccaagtat tttatcttta
     5941 tatgaaagta atcctctgta ttttcagcat tgtccaccag agccaaattt tgcaactgta
     6001 aaagaggaca tgctttgtct acctgaaggt aaagctaagg ctgataagtt ttttgttgga
     6061 ttttggaatg gctctgacct tgtggctgtt atggattttg tctatgcata tcctgatgag
     6121 gagactgttt ttattggttt gtttatggtt gatcaagcct atcagaggaa agggattggt
     6181 agtcatattg tgacagaagc actagcttat tttgctaaga actttcgaaa ggcacgtttg
     6241 gcttatgtta agggaaatcc gcaatctcag catttttggg aaaagcaggg ctttaaatca
     6301 attggatgcg aggttaagca agaactctat acggttgtta tcgctgaaca gagcctagaa
     6361 gattagaaat ggcatcaagt aagaactatt tggaatttgt tttggaacaa ttatcaggat
     6421 tagatgatgt gacttaccgt tccatgattg gggagtatat tctttacttc cgcggcaaga
     6481 ttattggcgg catttatgac gatcgctttt tagttaaacc cgtgcaagca gtcttagata
     6541 agattgacca atcttctttt gagtttccat acaaaggtgc caaagaaatg atttgagtgg
     6601 aagaacttga taataagatg tttctataag acctgatttt agctatgtat aaccaactgc
     6661 caacgcccaa acctaaaaag aaaaagcaag ggtgaacgaa gtaaaaaaga agtctgctaa
     6721 ggccctgtct ttgcacgggt aaaattttat atataaaaag aagctgggac taaagagctc
     6781 agcttccttt ggtttatata attgtcatta caagacgaag tggttgggcg aaactctgtt
     6841 gactttattc aatttagagt ttcttatgca caattgagtc tggaacgaaa gtctccagtt
     6901 gcaaagtata cagtacaata aaccaacgat gtaatagctg atgacacaaa gcacagtggg
     6961 taggacttgc gaagtcaccc ttttcttttc aaaatttata ctaaatcatt gatatcagtg
     7021 tagtcacgat taagtccttg agcaactggt aggctagtca agtaaccttg ataagtggtc
     7081 acaccttgac gcaagccttc atcttcagag attgcttgtg cgaatccttt gccagccaaa
     7141 gcttcgatat aaggaagagt gacattggtt agggcgatgg ttgaagtgcg ggcaaccgca
     7201 ccagggatat tggcaacggc atagtggaga acaccgtgtt tttcatagac gggttcatcg
     7261 tgcgttgtca cacggtcagc tgtttcgata acgccacctt ggtcaacagc aacgtcaacg
     7321 atacagagcc tggacgcatt tgtttgacca tctcatctgt caccaattcc ggtgcttttg
     7381 caccagggat gagaatggct ccaatcacca catcagcatc tctcacactt gcttcaatgt
     7441 tgaatgaatt agatataata gtttgaattt gacttccaaa gacttcttct agaactgaga
     7501 gacgcttggc actaatatct aaaatagtca cttgagcacc aagaccaagg gcgatgcggg
     7561 cagcatgtgt accgacgaca ccatcaccga tgatagttac ttttcctttt ggaacacctg
     7621 gtacaccacc aagtagaaca ccagagccac cagcttgctt agtaaggaag tgagctccga
     7681 tttgaacagc catacgacct gcaacctcac tcataggaac gaggagcggt agttgtcctt
     7741 gattgtcacg aacagtttca taggcaattc ctgttgtttt tgctgctaac atagcatctg
     7801 ctaattctgg agcagcggcc atgtgcaagt aggtgaagag aagaagatcg tcgcgcaagt
     7861 aaccgtattc agaacttaaa ggttctttta ctttcacaac caactctgct gcccaagctt
     7921 caccagcagt agcgacaatc tcagctcctt gcttttgata gtcagcatca gtaaagccag
     7981 aaccgagacc agcatttgtt tcgataagga cacgatgacc acgactaact aagctatgaa
     8041 cacctgcagg tgtgagggcg acacggtttt cgttattttt aatttctttt gggattccga
     8101 ttaacattga gataacctac ctttcaattg acggtcttgt tttggttgtc acattccagt
     8161 tcataaatca aaaatgtgac ggtttcattg tatatgaaac cgcttcaaaa atcaagaaaa
     8221 acttgtcatc caaatttttt tatgctagac tagtgaaaat caagctctaa tggagggaaa
     8281 agtatggaat caatatttgt gaaatttgcc cagtatccgt ctatagaaac ggagcgttta
     8341 ttgcttagac ctgtaacttt ggatgatgcg gaagcaatgt ttgactatgc ctcggacaag
     8401 ggtaatacac gttacacttt tccaaccaat caaagcttgg aagaaaccaa gaataacatt
     8461 gctcagttct acttggctaa tcccttggga cgttggggga ttgaattaaa agataacggc
     8521 aagtttatcg gaactattga cttacacaag attgatcctg ttcttaagaa ggcagctatt
     8581 ggctacatta tcaataaaaa gtattggaat caaggattaa cgacagaagc caatcgtgct
     8641 gtgattgagc tagcttttga gaagataggg atgaataagt tgactgccct tcacgataag
     8701 gctaatcccg cgtcaggaaa ggtcatggag aaatcaggca tgcgtttttc ccatgcagaa
     8761 ccatatgctt gtatggacca gcatgaaaaa ggccgaatcg tgacaagagt tcattatgtc
     8821 ttgaccaagg aagactattt tgcaaataaa taagcagttg aaaagaaatt tttcgactgt
     8881 tttttcttcc tcttacgaat aatctaagag aggagaaaat atggaagcaa ttatcgagaa
     8941 aatcaaagag tataaaatca tcgtcatctg tactggtctg ggcttgcttg taggaggatt
     9001 tttcctgcta aagccagctc cacaaacatc tgtcaaagag acgaatttgc aggctgaagt
     9061 tgcagctgtt tccaaggact catcgaccgg aaaggaagtg aacaaggaag agaaggaaga
     9121 accagttgaa caagatctaa tcacagtaga tgtcaaaggt gctgtcaaat cgccagggat
     9181 ttatgacttg cctgtaggta gtcgagtcaa tgatgctgtt cagaaggctg gtggcttgac
     9241 agagcaagca gacagcaagt cgctcaatct agctcagaaa gttagtgatg aggctctggt
     9301 ttacgttcct actaagggag aagaagcagt tagtcaacag actggttcgg ggacagcttc
     9361 ttcaacaagc aaggaaaaga aggtcaatct caacaaggcc agtctggaag aactcaagca
     9421 ggtcaaggga ctgggaggaa aacgagctca ggacattatt gaccatcgtg aggcaaatgg
     9481 caagttcaag tcagtagacg agctcaagaa ggtctctggc attggtgcca agaccataga
     9541 aaaacttaaa gactatgtta cagtggatta agaatttctc tattccccta atttacctga
     9601 gttttctatt actatggctt tactacgcca ttttctcagt atcctatttt gctttgttgg
     9661 gttttgtttt tctgctagtc tgcctcttta tccaatttcc ttggaaatca gcaggtaaag
     9721 ttctagtgat ttgtggagtc tttggcttct ggtttctgtt tcaaacttgg caacagagtc
     9781 aagtgagtca aaatttggtg gattctgttg aaagggtacg gattttacca gacactatta
     9841 aggttaacgg tgacagtctg tcctttcgtg gtaagtctga tggtcgtgct ttccaagtct
     9901 attataaact ccagtccgag gaggagaaag aagcctttca agctttaact gaccttcatg
     9961 agataggact agaagggaag ctttcggagc cagaagggca gagaaatttt ggtggctttg
    10021 actaccaagg ctatctgaag actcagggaa tttaccagac actcaatatt aaaagaatcc
    10081 agtcactcca aaaggttggc agttgggata taggtgaaaa cctgtccagt ttacgtcgaa
    10141 aggctgtggt ttggattaag atgcacttcc cagaccctat gcgcaattac atgacaggac
    10201 tcttgctagg acatctggac accgattttg aggagatgaa tgagctttat tccagtttag
    10261 gaattattca cctttttgcc ttgtcaggta tgcaggtagg gtttttcatg gacggattta
    10321 agaaactact tttacgattg ggcttgacac aagaaaagtt gaagtggctg acttatccct
    10381 tttcccttat ctatgcggga ctaactggat tttcagcatc ggttattcgc agtctcttgc
    10441 aaaagctact ggctcaacat ggggttaagg gcttggataa ttttgccttg acggtgcttg
    10501 tcctctttat tgtcatgcca aactttttct tgacagcagg aggagtcttg tcctgtgctt
    10561 atgcttttat cctgaccatg accagcaaag aaggggaggg gctcaaggct gttactagtg
    10621 aaagtctagt catctccttg ggcatattgc ccattctatc cttctatttt gcggaatttc
    10681 aaccttggtc tatccttttg acctttgtct tttcctttct ttttgacttg gtcttcttac
    10741 cgctcttgtc tatcttattt gtcctttcct ttctctatcc agtcattcag ctgaacttta
    10801 tctttgaatg gttagagggc attattcgct tggtctcgca ggtggcaagt agaccgcttg
    10861 tctttggaca acccaatgaa tggcttttaa tcctattgtt aatttctttg gctttggtct
    10921 atgatttgag gaaaaacatt aaaaagctaa cggtattgtg cttattgatt acagggctct
    10981 ttctcctgac caagcatcca ctggaaaatg aaatcaccat gctggatgtg gggcaaggag
    11041 aaagtatttt cctacgggat gtaactggga aaaccattct catagatgta ggtggtaagg
    11101 cagaatctta taagaaaatc aaaaaatggc aagaaaagat gacgaccagc aatgcccagc
    11161 gaagcttgat accctatctc aaaagtcgag gagtagctaa gattgaccag ctaattttga
    11221 ctaacacgga caaggagcat gttggagatt tgtcagagat gaccaaggct ttccatgtag
    11281 gggagattct agtatcaaaa gacagtctga aacagaagga atttgtggca gaactacagg
    11341 cgactcaaac aaaggtgcgt agtatgatag taggggagaa cttacccatt tttggaagtc
    11401 agttagaagt tctatctcca aggaaaatgg gagatggagg acacgatgat accctagttc
    11461 tgtatgggaa attcttggat aagcaatttc tcttcacggg aaatttggag gagaaaggag
    11521 agaaggactt gctgaagcac tatccagact tgaaagtaaa tgttttgaaa gctagccaac
    11581 atggcaataa aaaatcatca agtccagcct ttctagaaaa actcaaacca gagcttactc
    11641 ttatctcagt tggaaagagc aatcgaatga aactccccca tcaggaaaca ttgacacgac
    11701 tggaaggtat caatagcaaa gtttatcgaa ctgaccagca aggagctata cgttttaagg
    11761 ggttggatag ttggaaaatc gaaagtgttc gataggaagg ataaatgttg tagattagtg
    11821 aaataaacta aaaatttgtt gcataataat gataaaaacg gcataatgaa aacgtattca
    11881 atattgagga tataaaatca ttaaaaatca gcaaaagttg ttttagttag ttagtttata
    11941 atctattggt cttcttcagt ccagtgtatc tgctgtgaca gtcactaaaa gttacaagta
    12001 tgattggaat acggttttgg aatatagtac caactatcac gaccatcagt atgcttggat
    12061 tccggaatgg tctcgttatt acagctattc tgagtataaa gttggcggag gctggaacta
    12121 cgctcgttat gaggtcataa actactatac tggaggttat taatccttaa agagtgagaa
    12181 aaggagggct ggatatgtta aatcttactc atgttacctt aaaaacgcga caagtcatct
    12241 tgcaagatgc ggattttacc tttaaaaagg gtaggattta tggccttctt gctatcaatg
    12301 gctcgggaaa gacgacacta ttccgagcta tgagcaagtt gcttcccctt agtagtggac
    12361 acatcgcagt tcctccttct ttgttttatt atgagagcgt tgaatggctg gatggaaact
    12421 taagtgggat ggactacctt cgtctcataa aaaacatctg gaagtcagac ctaaacttga
    12481 gagatgaaat cgcctactgg gaaatggctg actatatcag tcttcccatc cgcaagtatt
    12541 ccttagggat gaagcaacgc ttggtgattg ctatgtattt tctcagtcag gccaaatgct
    12601 ggctcatgga tgagattaca aatggcttag acgagtatta tcgacagaag ttttttgata
    12661 ggctagcaca aatcgataga caagaacagc tggttctttt aagttcccac tataaggaag
    12721 agttggttga tatctgcgat agagtagtaa ccattcatca ggggcagata gaagaggttt
    12781 agtttatgaa agatgttagt ctatttttat tgaaaaaagt tttcaaaagt cgtttaaact
    12841 ggattatctt agctttattt gtatctgtac tcggtgttac cttttattta aatagtcaga
    12901 ctgcaaactc acacagcttg gagagcaggt tggaaagtcg cattgcagcc aacgagaggg
    12961 ctatcaatga aaatgaagag aaactctccc aaatgtctga taccagctcg gaggaatacc
    13021 agtttgctaa aaataattta gacgtgcaaa aaaatctttt gacgcgaaag acagaaattc
    13081 taactttact aaaagaaggg cgctggaaag aagcctacta tctgcagtgg aaagatgaag
    13141 agaagaatta tgaatttgta tcaaatgacc cgactgctag ctctggctta aaaatggggg
    13201 ttgaccgcga acggaagatt taccaagccc tgtatccctt gaacataaaa gcacatactt
    13261 tggagtttcc gacccacggg attgatcaga ttgtctggat tttagaggtt atcatcccaa
    13321 gcttgtttgt ggttgcgatt atttttatgc taacacaact atttgcagaa agatatcaaa
    13381 atcatctgga cacagctcac ttatatcctg tttcaaaagt gacatttgca atatcctctc
    13441 ttggagttgg agtgggatat gtaactgtgc tgtttatcgg aatctgtggc ttttcttttc
    13501 tagtgggaag tctgataagt ggttttggac agttagatta tccctaccca atttatagct
    13561 tagtgaatca agaagtaact attgggaaaa tacaagatgt attatttcct ggcttgctct
    13621 tagctttctt agcctttatc gtcattgtgg aagttgtgta cttgattgct tactttttca
    13681 agcaaaaaat gcctgtcctc tttctttcac tcattgggat tgttggctta ttgtttggca
    13741 tccaaacaat tcagcctctt caaaggattg cacatctgat tccctttact tacttgcgtt
    13801 cagtggagat tttatctgga agattaccta agcagattga taatgtcgat ctaaattgga
    13861 gcatgggaat ggtcttactt ccttgcctga ttatcttttt gctattggga attctattta
    13921 ttgaaagatg gggaagttca cagaaaaaaa gaatttttta atagattcta gctttcctat
    13981 aggtagggaa aataagtaaa aactaacata gagagggaat caacttgatt ctctcttttt
    14041 gattcgaaaa ccaaaccaaa atacaaacac aaacttttca aaaaataact ttttatcttg
    14101 acaagagcta gaaaacttgg tatcatataa aagttgagaa aagcagaagt gagagcttct
    14161 cgccttgtga cattaagttg cctggcccta cggatgaaaa gtttcgaaga aacgctatca
    14221 taacgtgcgg gcttgtatat ttacgagtcc gctattgttt ttctctaata aaacaaaaga
    14281 ggtgaaaacc atagcaaagc aagacttatt catcaatgat gagattcgtg tacgtgaagt
    14341 tcgcttgatt ggtcttgaag gagaacagct aggcatcaag ccactcagtg aagcgcaagc
    14401 tttggctgat aacgctaatg ttgacctagt attgattcaa ccccaagcca aaccgcctgt
    14461 tgcaaaaatt atggactacg gtaagttcaa atttgagtac cagaagaagc aaaaagaaca
    14521 acgtaaaaaa caaagcgttg ttactgtgaa agaagttcgt ctaagtccaa ctattgacaa
    14581 gggtgacttt gatacaaaac ttcgtaatgc acgtaagttc cttgaaaaag gaaataaagt
    14641 taaggtatct attcgcttta agggtcgtat gattacccat aaagagattg gtgcaaaagt
    14701 tttagccgag tttgctgaag caactcaaga tattgccatc atcgaacaac gtgctaaaat
    14761 ggatggacgt caaatgttca tgcaattggc gccagcgact gacaaaaaat aattgtcaga
    14821 aagttaaata aaggagaaaa aattatgcca aaacaaaaaa cacaccgcgc atcagctaaa
    14881 cgtttcaaac gtacaggttc tggtggactt aaacgtttcc gtgcttacac ttctcaccgt
    14941 ttccacggaa aaactaagaa acaacgtcgt catcttcgta aagcatctat ggtgcattca
    15001 ggagattaca aacgtatcaa agcaatgctt actcgcttga aataatagct tgactgtaag
    15061 taaacaattc taggaaatat ttggaggaaa taaaacatgg cacgtgttaa aggtggcgtt
    15121 gtatcacgca aacgtcgtaa acgtattctt aaattagcaa aaggttacta tggagctaaa
    15181 cacatcttgt tccgtactgc aaaagaacaa gtaatgaact cttactacta tgcataccgt
    15241 gaccgtcgtc agaaaaaacg tgacttccgc aaattgtgga tcactcgtat caatgcggca
    15301 gctcgtatga acggactttc atactcacaa ttgatgcatg gtttgaaatt ggctgagatc
    15361 gaagttaacc gtaaaatgct tgctgacttg gctgttaacg atgcagtagc tttcacagct
    15421 cttgcagatg cagctaaagc aaaacttggt aaataacaac tgatgagact ggaacaggat
    15481 tgtccagtct ttttaaaaat aaaggagaaa aatatggctt caaaaatgct acacacttgc
    15541 ttacgagtag aaaatcttga aaaatcaatc gcattttatc aagatgcttt tggttttaaa
    15601 gaattgcgtc gcagagattt tccagaccat gccttcacga ttgtctatct aggtcttgag
    15661 ggtgatgact atgagttgga gttgacttat aactacgatc acggtccgta tgtggttggt
    15721 gatgggtttg cccatatcgc cctcagtaca cctgatcttg aggcacttca tcaagaacac
    15781 agtgcaaaag gctatgaagt gactgagcca aatggtctac caggaactac accaaactat
    15841 tactttgtca aagaccctga tggatataag gtcgaagtca ttcgtgaaaa ataatttcgt
    15901 gcaagttaag tagaatattc gttttttact tgactttttt tagctgaaag agtatactaa
    15961 taaaaattca acctttaagg ggagtccaga gagactcaca aggtgtcaga taaaagaata
    16021 gtgcaatttt ctagaggaga ctttttgagt gtgctctctt gtgttgtacg attttaactg
    16081 aggccttgca ctagcaaggt cttttcttta tctggtcccc ttaaaattta aggaggaaaa
    16141 gttatgaatc ccacatgtaa gaagcgtttg ggtgtcattc ggttggaaac catgaaggtg
    16201 gttgcacaag aggaaatcgc gccagcaatc tttgaattag tcctagaagg agaaatggtt
    16261 gaagccatgc gagcaggcca atttcttcat ctgcgtgtac cagacgatgc ccatctttta
    16321 cgtcgtccta tttcaatttc gtctattgac aaggcaaaca agcagtgtca cctcatttat
    16381 cggattgagg gagctgggac tgcaattttt tcaaccttaa gtcagggaga cactcttgat
    16441 gtgatggggc ctcagggaaa tggttttgac ttgtctgacc ttgatgagca gaatcaggtt
    16501 ctccttgttg gtggtgggat tggtgttcca cccttgcttg aggtggccaa ggaattgcat
    16561 gaacgtggag tgaaagtagt gacagtcctc ggttttgcta ataaggatgc tgttattttg
    16621 aaaacggaat tggctcagta tggtcaggtc tttgtaacga cagatgatgg ttcttatggc
    16681 atcaagggaa atgtttccgt tgttatcaat gatttagata gtcagtttga tgctgtttac
    16741 tcgtgtgggg ctccaggaat gatgaagtat atcaatcaaa cctttgatga tcacccaaga
    16801 gcctatttat ctctggaatc tcgtatggct tgtgggatgg gagcttgcta tgcctgtgtt
    16861 ctaaaagtac cagaaagcga gacggtcaac caacgcgtct gtgaagatgg tcctgttttc
    16921 cgcacaggaa cagttgtatt ataaggagaa aattatgact acaaatcgat tacaagtgtc
    16981 tctacctggt ttggatttga aaaatccgat tattccagca tcaggttgtt ttggctttgg
    17041 acaagagtat gccaagtact atgatttaaa ccttttaggt tctattatga tcaaggcgac
    17101 aacccttgaa ccacgttttg ggaacccaac tccaagagta gcagagacgc ctgctggtat
    17161 gctcaatgca attggcttgc aaaatcctgg tttagaggtt gttttggctg aaaagctacc
    17221 ttggctggaa agagaatatc caaatcttcc tattattgcc aatgtagctg gtttttcaaa
    17281 acaagagtat gcagctgttt ctcatgggat ttccaaggca actaatgtaa aagctatcga
    17341 gctcaatatt tcttgtccca atgttgacca ctgtaatcat ggacttttga ttggtcaaga
    17401 tccagatttg gcttatgatg tggtgaaagc agctgtggaa gcctcagaag tgccagttta
    17461 tgtcaaatta accccgagtg tgaccgatat cgttactgtc gcaaaagctg cagaagatgc
    17521 gggagcaagt ggcttgacca tgatcaatac tctggttgga atgcgctttg acctcaaaac
    17581 cagaaaacca atcttggcca atggaacagg tggaatgtca ggtccagcag tctttccagt
    17641 agccctcaaa ctcatccgcc aagtagccca aacaacaaac ctgcctatca ttggaatggg
    17701 gggagtggat tcggctgaag ctgccctaga aatgtatctg gctggggcat ctgctatcgg
    17761 agttggaaca gctaacttta ccaatcctta tgcctgccct gacatcatcg aaaatttacc
    17821 aaaagtcatg gataaatacg gtattagcag tctggaagaa ctccgtcagg aagtaaaaga
    17881 gtctctgagg taaactgcaa tcaatctgtt cttgattttt tattagtttg taatatgaat
    17941 ttaggagaat tttggtacaa taaaataaat aagaacagag gaagaaggtt aatgaagaaa
    18001 gtaagattta tttttttagc tctgctattt ttcttagcta gtccagaggg tgcaatggct
    18061 agtgatggta cttggcaagg aaaacagtat ctgaaagaag atggcagtca agcagcgaat
    18121 gagtgggttt ttgatactca ttatcaatct tggttctata taaaagcaga tgctaactat
    18181 gctgaaaatg aatggctaaa gcaaggtgac gactattttt acctcaaatc tggtggctat
    18241 atggccaaat cagaatgggt agaagacaag ggagcctttt attatcttga ccaagatgga
    18301 aagatgaaaa gaaatgcttg ggtaggaact tcctatgttg gtgcaacagg tgccaaagta
    18361 atagaagact gggtctatga ttctcaatac gatgcttggt tttatatcaa agcagatgga
    18421 cagcacgcag agaaagaatg gctccaaatt aaagggaagg actattattt caaatccggt
    18481 ggttatctac taacaagtca gtggattaat caagcttatg tgaatgctag tggtgccaaa
    18541 gtacagcaag gttggctttt tgacaaacaa taccaatctt ggttttacat aaaagaaaat
    18601 ggaaactatg ctgataaaga atggattttc gagaatggtc actattatta tctaaaatct
    18661 ggtggctaca tggcagccaa tgaatggatt tgggataagg aatcttggtt ttatctcaaa
    18721 tttgatggga aaatagctga aaaagaatgg gtctacgatt ctcatagtca agcctggtac
    18781 tacttcaaat ctggtggtta catggcgaaa aatgagacag tagatggtta tcagcttgga
    18841 agcgatggta aatggcttgg aggaaaagct acaaatgaaa atgctgctta ctatcaagta
    18901 gtgcctgtta cagccaatgt ttatgattca gatggtgaaa agctttccta tatatcgcaa
    18961 ggtagtgtcg tatggctaga taaggataga aaaagtgatg acaagcgctt ggctattact
    19021 atttctggtt tgtcaggcta tatgaaaaca gaagatttac aagcgctaga tgctagtaag
    19081 gactttatcc cttattatga gagtgatggc caccgttttt atcactatgt ggctcagaat
    19141 gctagtatcc cagtagcttc tcatctttct gatatggaag taggcaagaa atattattcg
    19201 gcagatggcc tgcattttga tggttttaag cttgagaatc ccttcctttt caaagattta
    19261 acagaggcta caaactacag tgctgaagaa ttggataagg tatttagttt gctaaacatt
    19321 aacaatagcc ttttggagaa caagggcgct acttttaagg aagccgaaga acattaccat
    19381 atcaatgctc tttatctcct tgcccatagt gccctagaaa gtaactgggg aagaagtaaa
    19441 attgccaaag ataagaataa tttctttggc attacagcct atgatacgac cccttacctt
    19501 tctgctaaga catttgatga tgtggataag ggaattttag gtgcaaccaa gtggattaag
    19561 gaaaattata tcgatagggg aagaactttc cttggaaaca aggcttctgg tatgaatgtg
    19621 gaatatgctt cagaccctta ttggggcgaa aaaattgcta gtgtgatgat gaaaatcaat
    19681 gagaagctag gtggcaaaga ttagtactat aagtgaatat gatttgagtg aatagtaagt
    19741 taaaaatcct gatttcaagt aaaatcagga ttttttcatg gatgcaattt ttttggagtc
    19801 tggtgtgacg cggagggtct tttgtcctgt gtaagtgaca aagccgggtt ttccaccagt
    19861 tggtttattg agttttttga cttcaatcat atctacctgc accagattcg acaggcgccc
    19921 ttgagagaag taggcagcta actctgctgc gtctgtcttg actgcatcag atgggtcaag
    19981 atttcctgag atgacaacat ggcttccagg aatgtcctta gcatggaacc aaagttcctc
    20041 cttgcgggcc attttaaagg tcaattcctc attttgaaga ttgtttcgtc cgacatagat
    20101 gatggttttg ccatcgcttg ctagatattg ttctagtttt ttgcgtttct ggattttctc
    20161 ccgttgtctt ctgcggataa aacctgtttg aatcaattct tcacggattt cagcgatttc
    20221 ttccagtcca gcttggttga ggacggtttc tacactttcc agatagagaa tagtggcttt
    20281 ggtttcttca atcaaatcag tcaagtattt gacagcttct ttgagtttct gataccgttt
    20341 aaaatagcgt tgggcattct ggttgggagt cagagcctta tcaagcgcaa tcatgatagg
    20401 ttggttggta tagtagttgt ctaggataac ctggtcttgg tcgttaggca cttggtggag
    20461 gaaggttgtc agcaattctc ctttttgacg aaattcttca gcgttgtctg tcgccagtaa
    20521 ctctttttcc tgttttttga gtttgtgtcg gtttttctga agttcatttt caacacgacg
    20581 aatcagttca ctggcctgct gtttgacgcg gtcgcgctca gccttatcct tatagtaggt
    20641 gtccaacaaa tcagaaagat ttgcaagagg ctctcccacc tgatttgcaa aaggaactgg
    20701 actgaaggaa gtctcagtca agcatggctt ggtttcttga ttgaaaaaat ttcggaaagc
    20761 ggaaagtttt tcactaacca gtatcctttc caattcattt gccgtatcgc gtcccagacc
    20821 ttgaaagagg ctttgaagat tttttgctgt tagttcttgg gtttgcagga tttcaaagag
    20881 cttttcatcc ttgatagtaa aaggattgag agattttgta cttggcggag cgatataggt
    20941 cgatcctgga agtaaggtgc ggtagctatt ttgtgaaaag ccgacgtgtt tgataacttc
    21001 gaggatttta tgactgcttt tatcgaccag tagaatatta ctgtgtttcc ccataatttc
    21061 gataatcaag gtagcctgga tatggtctcc aatctcgttt ttattggaaa ctgtaatttc
    21121 cacaatacgg tcattttcca cttgctcaat cgactcaatc agggccccct gcaaatactt
    21181 tctcaaaacc atgataaagg tagaaggttg ggctggattt tcaaaagtcg tttgggtcag
    21241 ctgaatgcgt ccaaaaactg gatgggcaga aaggagcagg cgatggcttt ggcgattgct
    21301 gcggatttgc aagaccaact cttgttcaaa aggctgattg attttctgga tgcgaccatt
    21361 cactaattcg cttcgcaatt cctcaactat gtggtgtaaa aaaaatccgt caaatgacat
    21421 cgttctctcc ttgtgattgt attccatagt attatatcaa aaaggtagaa taaaatcatg
    21481 gaaatgtggt ataataaagc caagtaaaga gaaacgagaa gcacatgtat attgaaatgg
    21541 tagatgaaac tggtcaagtt tcaaaagaaa tgttgcaaca aacccaagaa attttggaat
    21601 ttgcagccca aaaattagga aaagaagaca aggagatggc agtcactttt gtgaccaatg
    21661 agcgtagtca tgaacttaat ctggggtacc gtgacaccga ccgtccgaca gatgtcatca
    21721 gccttgagta taaaccagaa ttggaaattg cctttgacga agaggatttg cttgaaaatc
    21781 cagaattggc agagatgatg tctgagtttg atgcctatat tggggaattg ttcatctcta
    21841 tcgataaggc tcatgagcag gccgaagaat atggtcacag ctttgagcgt gagatgggct
    21901 tcttggcagt acacggcttt ttacatatta acggctatga tcactatact ccggaagaag
    21961 aagcggagat gttcggttta caagaagaaa ttttgacagc ctatggactc acaagacaat
    22021 aaacgaaaat ggaaaaatcg tgacctgata tccagtttag aatttgccat cacagggatt
    22081 tttactgcta tcaaggaaga acgcaatatg cgaaaacatg cagtgacggc tctagtggtc
    22141 atccttgcag gttttggttt tcaggtgtca cgaatcgaat ggctctttct cctattgagc
    22201 attttcttgg tggtagcctt tgaaattatc aattctgcta tcgaaaatgt ggtggatttg
    22261 gccagtcact atcacttttc catgctggct aaaaatgcca aggatatggc ggctggcgcg
    22321 gtattagtgg tttctctttt tgcagcctta acaggcgcat tgatttttct cccacgaatc
    22381 tgggatttat tattttaaac agtaagagga aattatgact tttaaatcag gctttgtagc
    22441 cattttagga cgtcccaatg ttgggaagtc aaccttttta aatcacgtta tggggcaaaa
    22501 gattgccatc atgagtgaca aggcgcagac aacgcgcaat aaaatcatgg gaatttacac
    22561 gactgataag gagcaaattg tctttatcga cacaccaggg attcacaaac ctaaaacagc
    22621 tctcggagat ttcatggttg agtctgccta cagtaccctt cgcgaagtgg acactgttct
    22681 tttcatggtg cctgctgatg aagcgcgtgg taagggggac gatatgatta tcgagcgtct
    22741 caaggctgcc aaggttcctg tgattttggt ggtgaataaa atcgataagg tccatccaga
    22801 ccagctcttg tctcagattg atgacttccg taatcaaatg gactttaagg aaattgttcc
    22861 aatctcagcc cttcagggaa ataacgtgtc tcgtctagtg gatattttga gtgaaaatct
    22921 ggatgaaggt ttccaatatt tcccgtctga tcaaatcaca gaccatccag accgtttctt
    22981 agtttcagaa atggttcgcg agaaagtctt gcacctaact cgtgaagaga ttccgcattc
    23041 tgtagcagta gttgttgact ctatgaaacg agacgaagag acagacaagg ttcacatccg
    23101 tgcaaccatc atggtcgagc gcgatagcca aaaagggatt atcatcggta aaggtggcgc
    23161 tatgcttaag aaaatcggta gcatggcccg tcgtgatatc gaactcatgc taggagacaa
    23221 ggtcttccta gaaacctggg tcaaggtcaa gaaaaactgg cgcgataaaa agctagattt
    23281 ggctgacttt ggctataatg aaagagaata ctaagtagag gtgggctcat gcctgcttct
    23341 tgtttttaca gaaggaggac ttatgcctga attacctgag gttgaaaccg tttgtcgttg
    23401 cttagaaaaa ttgattatag gaaagaagat ttcgagtata gaaattcgct accccaagat
    23461 gattaagacg gatttggaag agtttcaaag ggaattgcct agtcagatta tcgagtcaat
    23521 gggacgtcgt ggaaaatatt tgctttttta tctgacagac aaggttttga tttcccattt
    23581 gcggatggag ggcaagtatt tttactatcc agaccaagga cctgaacgca agcatgccca
    23641 tgttttcttt cattttgaag atggtggcac gcttgtttat gaggatgttc gcaagtttgg
    23701 aaccatggaa ctcttggtgc ctgacctttt agacgcctac tttatttcta aaaaattagg
    23761 tcctgaacca agcgaacaag actttgattt acaggtcttt caatctgccc ttgccaagtc
    23821 caaaaagcct atcaaatccc atctcctaga ccagaccttg gtagctggac ttggcaatat
    23881 ctatgtggat gaggttctct ggcgagctca ggttcatcca gctagatctt cccagacttt
    23941 gacagcagaa gaagcgacag ctattcatga ccaaaccatt gctgttttga gccaggctgt
    24001 tgaaaaaggc ggttccacca ttcggaccta taccaatgcc tttggggaag atggaagcat
    24061 gcaggatttt catcaggtct atgataagac tggtcaagga tgtgtacgct gtggtaccat
    24121 cattgagaaa attcaactag gtggacgtgg aacccacttt tgtccaaact gtcaaaggag
    24181 ggactgatgg gaaaaatcat cggaatcact gggggaattg cctctggtaa gtcaactgtg
    24241 acaaattttc taagacagca aggctttcaa gtagtggatg ccgacgcagt cgtccaccaa
    24301 ctacagaaac ctggtggtcg tctgtttgag gctctagtac agcacttttg gcaagaaatc
    24361 attcttgaaa acggagaact caatcgccct ctcctagcta gtctcatctt ttcaaatcct
    24421 gatgaacgag aatggtctaa gcaaattcaa ggggagatta tccgtgagga actggctact
    24481 ttgagagaac agttggctca gacagaagag attttcttca tggatattcc cctacttttt
    24541 gagcaggact acagcgattg gtttgctgag acttggttgg tctatgtgga ccgagatgcc
    24601 caagtagaac gcttaatgaa aagggaccag ttgtccaaag atgaagctga gtctcgtctg
    24661 gcagcccagt ggcctttaga aaaaaagaaa gatttggcca gccaggttct tgataataat
    24721 ggcaatcaga accagcttct taatcaagtg catatccttc ttgagggagg taggcaagat
    24781 gacagagatt aactggaagg ataatctgcg cattgcctgg tttggtaatt ttctgacagg
    24841 agccagtatt tctttggttg taccttttat gcctatcttc gtggaaaatc taggtgtagg
    24901 gagtgagcaa gtcgcttttt atgcaggctt agcaatttct gtctctgcta tttccgcggc
    24961 gctcttttct cctatttggg gtattcttgc tgacaaatac ggccgaaaac ccatgatgat
    25021 tcgggcaggt cttgctatga ctatcactat gggaggcttg gcctttgtcc caaatatcta
    25081 ttggttaatc tttcttcgtt tactaaacgg tgtatttgca ggttttgttc ctaatgcaac
    25141 ggcactgata gccagtcagg ttccaaagga gaaatcaggc tctgccttag gtactttgtc
    25201 tacaggcgta gttgcaggta ctctaactgg tccctttatt ggtggcttta tcgcagaatt
    25261 atttggcatt cgtacagttt tcttactggt tggtagtttt ctatttttag ctgctatttt
    25321 gactatttgc tttatcaagg aagattttca accagtagcc aaggaaaagg ctattccaac
    25381 aaaggaatta tttacctcgg ttaaatatcc ctatcttttg ctcaatctct ttttaaccag
    25441 ttttgtcatc caattttcag ctcaatcgat tggccctatt ttggctcttt atgtacgcga
    25501 tttagggcag acagagaatc ttctttttgt ctctggtttg attgtgtcca gtatgggctt
    25561 ttccagcatg atgagtgcag gagtcatggg caagctaggt gacaaggtgg gcaatcatcg
    25621 tctcttggtt gtcgcccagt tttattcagt catcatctat ctcctctgtg ccaatgcctc
    25681 tagccccctt caactaggac tctatcgttt cctctttgga ttgggaaccg gtgccttgat
    25741 tcccggggtt aatgccctac tcagcaaaat gactcccaaa gccggcattt cgagggtctt
    25801 tgccttcaat caggtattct tttatctggg aggtgttgtt ggtcccatgg caggttctgc
    25861 agtagcaggt caatttggct accatgctgt cttttatgcg acgagccttt gtgttgcctt
    25921 tagttgtctc tttaacctga ttcaatttcg aacattatta aaagtaaagg aaatctagtg
    25981 cgagtaaaaa ttaatctcaa atgctcctct tgtggcagta tcaattacct aaccagtaaa
    26041 aattcaaaaa cccatccaga caagattgag gttttaaagt attgtcccaa ggaaagaaaa
    26101 gtaaccctac atcttgaatc taagtagatt ttatggtaaa ataataggga ttttaaggag
    26161 tttgatatgt ataacctatt attaaccatt ttattagtat tatctgttgt gattgtgatt
    26221 gcaattttca tgcaaccaac caaaaaccaa tccagcaatg tatttgatgc cagttcaggt
    26281 gatttgtttg aacgtagtaa agctcgcggt tttgaagctg taatgcagcg tttgacaggg
    26341 attttagtct ttttctggct agccattgcc ttagcattga cggtattatc aagtagataa
    26401 gaaaataatg ggcaggacta ggtctttgcc tctttttatt tttaaaggat gtttgagaag
    26461 gttttacagt aaaagaaaat taaaaaatct agaaagaaaa tatgaaagat agaataaaag
    26521 aatatttaca agacaaggga aaggtgactg ttaatgattt ggctcaggct ttgggaaaag
    26581 acagttccaa ggattttcgt gagttgatta aaaccttgtc cttaatggaa agaaagcacc
    26641 aaattcgttt tgaagaagat ggtagtctga cattagaaat taagaaaaaa catgagatta
    26701 ccctcaaggg gatttttcat gcccataaaa atggctttgg ctttgttagt ctggaaggcg
    26761 aggaggacga cctttttgta gggaaaaatg atgtcaacta tgctattgat ggtgataccg
    26821 tcgaggtagt gattaagaaa gtcgctgacc gcaataaggg aacagcagca gaagccaaaa
    26881 ttattgatat cctagaacac agtttgacaa cagttgtcgg gcaaatcgtt ctggatcagg
    26941 aaaaacctaa gtatgctggc tatattcgtt caaaaaatca gaaaatcagt caaccgattt
    27001 atgttaagaa accagcccta aaattagaag gaacagaagt tctcaaagtc tttatcgata
    27061 aatacccaag caagaaacat gatttctttg tcgcgagtgt tctcgatgta gtgggacact
    27121 caacggatgt cggaattgat gttcttgagg tcttggaatc aatggacatt gtatccgagt
    27181 ttccagaagc tgttgttaag gaagcagaaa gtgtgcctga tgctccgtct caaaaggata
    27241 tggaaggtcg tctggatcta agagatgaaa ttacctttac cattgacggt gcggatgcca
    27301 aggacttgga cgatgcagtg catatcaagg ctctgaaaaa tggcaatctg gagcttgggg
    27361 ttcacatcgc agatgtttct tattatgtga ccgaggggtc tgcccttgac aaggaagccc
    27421 ttaaccgtgc gacttctgtt tacgtgacag accgagtggt gccaatgctt ccagaacgac
    27481 tatcaaatgg catctgctct ctcaatcccc aagttgaccg cctgacccag tctgctatta
    27541 tggagattga taaacatggt cgtgtggtca actataccat tacacaaaca gttatcaaga
    27601 ccagttttcg tatgacctat agcgatgtca atgatatcct agctggcgat gaagaaaaga
    27661 gaaaagaata tcataaaatt gtatcaagta tcgaactcat ggccaagctt catgaaactt
    27721 tagaaaacat gcgtgtgaaa cgtggagctc tcaattttga taccaatgaa gcgaagattt
    27781 tagtggataa acaaggtaag cctgttgata tcgttcttcg gcagcgtggt attgccgagc
    27841 ggatgattga gtcttttatg ttgatggcta atgaaacagt tgccgaacat ttcagcaagt
    27901 tggatttgcc ttttatctat cgaattcacg aggagcctaa ggctgaaaag gttcagaagt
    27961 ttattgatta tgcttcgagt tttggcttgc gcatttatgg aactgccagt gagattagtc
    28021 aggaggcact tcaagacatc atgcgtgctg ttgagggaga accttatgca gatgtattgt
    28081 ccatgatgct tcttcgctct atgcagcagg ctcgttattc ggagcacaat cacggccact
    28141 atggactagc tgctgactat tatactcact ttaccagtcc aattcgtcgt tatccagacc
    28201 ttcttgttca ccgtatgatt cgggattacg gccgttctaa ggaaatagca gagcattttg
    28261 aacaagtgat tccagagatt gcgacccagt cttccaaccg tgaacgtcgt gccatagaag
    28321 ctgagcgtga agtcgaagcc atgaaaaagg ctgagtatat ggaagaatac gtgggtgaag
    28381 agtatgatgc agttgtatca agtattgtca aattcggtct ctttgtcgaa ttgccaaaca
    28441 cagttgaagg cttgattcac atcactaatc tacctgaatt ttatcatttc aatgagcgtg
    28501 atttgactct tcgtggagaa aaatcaggta tcactttccg agtgggtcag cagatccgta
    28561 tccgtgttga aagagcggat aaaatgactg gagagattga tttttcattc gtacctagtg
    28621 agtttgatgt gattgaaaaa ggcttgaaac agtctagtca tagtggcaga gggcgtggtt
    28681 caaatcgtcg ttcggataag aaggaagaca agagaaaatc aggacgctca aatgataagc
    28741 gtaagcattc acaaaaagac aagaagaaaa aaggaaagaa acctttttac aaggaagtag
    28801 ctaagaaagg agccaagcat ggcaaagggc gagggaaagg tcgtcgcaca aaataaaaag
    28861 gcacgccacg actatacaat cgtagatacg ctagaggcag ggatggtcct gactggaact
    28921 gaaatcaaga gtgtacgagc tgctcgaatt aatatcaagg atggctttgc tcaagtgaaa
    28981 aatggagaag tttggctgag caatgttcat atcgcgcctt acgaagaggg caatatctgg
    29041 aaccaggaac cagaacgtcg tcgtaaactc ctgctccata aaaagcaaat tcaaaaattg
    29101 gaacaagaga ccaaagggac aggaatgacc ttagttcccc ttaaggtcta tataaaagat
    29161 ggctacgcta agcttctttt aggacttgcc aaagggaagc atgactatga caaacgggag
    29221 tctatcaaac gtcgtgagca aaatcgagat atcgcgcgtg tgatgaaagc tgttaatcag
    29281 cgataaaaag aggaattgaa aatggaaaaa ttagttgcct ataaacgcat gcctttgtgg
    29341 aataaacaaa caatgcctga agatgttcag caaaagcaca atacaaaagt tgggacttgg
    29401 gggaaaatta ctgtcttgaa gggagctctc aagtttattg aattgacaga agaaggggaa
    29461 gttctagctg aacacctctt tgaagcaggg gcagacaatc caatggccca acctcaagcc
    29521 tggcaccgag tggaagctgc cacagatgat gtggaatggt acttggaatt ttattgtaaa
    29581 cctgaggatt attttgctaa aaaatacaat accaatcctg ttcattcaga ggtcctagag
    29641 gccatgcaga cagtgaaaca agggaaagct ttggatttgg gttgtggtca ggggcgtaat
    29701 tctctttttc tagcccagca agattttgat gtgacggctg tagatcaaaa tggactagct
    29761 cttgaaatct tgcaaagcat tgtggagcag gaagatttgg acatgcctgt tggcctttac
    29821 gatatcaatt cagctagcat tgaacaagaa tatgatttta tcgtttcaac agttgttctc
    29881 atgtttctac aagcggaccg cattccagct attattcaaa atatgcagga gaaaaccagt
    29941 gttggtggtt acaaccttat cgtttgtgcc atggacacgg aggattatcc ttgctcggtt
    30001 aatttcccat tcacctttaa agaaggagaa ctggcagact attacaagga ttgggaattg
    30061 gttaagtaca atgaaaatcc aggccatttg caccgtcgcg atgagaatgg caatcgtatt
    30121 caactacgct ttgcgacctt actagctaag aaaatcaagt aaacacacat gaagattagg
    30181 aattttcctg atcttttttc ttttttacga atgatataga aaaggaggga attcatgttt
    30241 gttgcgagag atgctagggg agaattggta aatgtgttag aggataaact tgagaagcaa
    30301 gcatacacct gcccagcttg tggaggccag ctccatttgc gtcaaggacc aagtgtacgg
    30361 acgcattttg cccataaatc cttaaaagac tgtgattttt tctttgaaaa tgaaagtcca
    30421 gaacacctgg ccaataagga atccctctat cactggttga aaaaagagac aaaggttcaa
    30481 ttagagtacc cgctttcaga acttaaacag attgcggatg tatttgtaaa tggcaatcta
    30541 gctctagaag ttcagtgtag tcccttgcct cagaaagtcc ttaaagagcg aagtgaggga
    30601 tatcgtagtc agggttacca agtactgtgg ttgctgggtc aaaaactgtg gctcaaggag
    30661 cgtttgactc gtctacagca aggttttctt tatttcagtc aaaacatggg cttttatgtt
    30721 tgggaattag acaaggaaaa acaagtttta agactcaaat acctgattta ccaggatctc
    30781 cgcggtaaac tccattatca aatcaaggaa ttttcctatg gtcaaggtag tttattggaa
    30841 atattgcgtc ttccctataa gaaacaaaaa atatctcatt ttacagtttc tgaggacaag
    30901 gacatctgtc gctatatccg gcaacaactt tattatcaaa atctcttttg gatgaaagaa
    30961 caagcagaag cctatcaaaa gggagaaaat atcctgactt atggactgaa agaatggtat
    31021 ccacaaattc gaccaatagt gggcaaattt ttccagattg aacaagactt gactagctat
    31081 tatcagcact tttataccta ttaccaaaaa aatcctcaaa atgattggca aaagctttat
    31141 ccaccagcct tttatcagca atatttcttg aaaaatatgg tagaatagaa aggatggagg
    31201 aatctaatgg tattacaaag aaatgaaata aatgaaaaag atacatggga tctatcaacg
    31261 atctacccaa ctgaccaggc ttgggaagaa gtcttaaaag atttaacaga acaattggag
    31321 acagtagccc agtatgaagg ccatctcttg gatagtgcgg ataacctact agaaatcact
    31381 gaattttctc ttgaaatgga acgccagatg gagaagcttt acgtttatgc tcatatgaag
    31441 aatgaccagg atacacgtga agctaagtat caagagtact atgccaaggc catgacattc
    31501 tacagccagt tagaccaagc cttttcattc tatgagcctg aatttatgga gattagcgaa
    31561 aagcagtatg ctgacttttt agaagctcaa ccaaagctgc aggtttatca acactatttt
    31621 gacaagctct tgcaaggcaa ggatcacgtt ctctcacaac gtgaagaaga attattggct
    31681 ggagctggag aaatctttgg ttcagcaagt gaaaccttcg ctatcttgga caatgcggat
    31741 attgtgttcc cttatgtcct agacgatgat ggtaaagaag ttcagctatc tcatgggatt
    31801 tacacacgtt tgatggagtc taaaaaacgt gaggttcgcc gtggtgccta tcaagctctt
    31861 tatgcgactt acgaacaatt ccaacacacc tatgccaaaa ccttgcaaac caatgttaag
    31921 gtgcaaaatt accgtgctaa agttcgtaac tacaagagtg ctcgtcatgc agccctcgca
    31981 gcgaattttg ttccagaaag tgtttatgac aatttggtag cagcagttcg caagcatttg
    32041 ccactcttac atcgctatct tgagcttcgt tcaaaaatct tggggatttc agatctcaag
    32101 atgtacgatg tctacacacc gctttcatct gttgaataca gttttaccta ccaagaagcc
    32161 ttgaaaaaag cagaagatgc tttggcagtc ttgggtgagg attacttgag ccgtgttaaa
    32221 cgtgccttca gcgagcgttg gattgatgtt tacgaaaatc aaggcaagcg ttcaggtgcc
    32281 tactctggtg gttcttacga taccaatgcc tttatgcttc tcaactggca ggacaatctg
    32341 gacaatctct ttactcttgt tcatgaaaca ggtcacagta tgcattcaag ctatactcgt
    32401 gaaactcagc cttatgttta cggagattac tctatctttt tggctgagat tgcctcaact
    32461 accaatgaaa atatcttgac ggagaaatta ttggaagaag tggaagacga cgcaacacgc
    32521 tttgctattc tcaataactt cctagatggt ttccgtggaa cagttttccg ccaaactcaa
    32581 tttgctgagt ttgaacacgc cattcaccaa gcagatcaaa atggggaggt cttgacaagc
    32641 gatttcctaa ataaactcta cgcagacttg aaccaagagt attatggttt gagtaaggaa
    32701 gacaatcctg aaatccaata cgagtgggct cgcattccac acttctacta taactactat
    32761 gtatatcaat attcaactgg ctttgcggcc gcctcagcct tggctgaaaa aattgtccat
    32821 ggtagtcaag aagaccgtga ccgctatatc gactacctca aggcaggtaa gtcggactat
    32881 ccacttaatg tcatgagaaa agctggtgtt gatatggaga aggaagacta cctcaacgat
    32941 gcctttgcag tctttgaacg ccgtttaaat gagtttgaag cccttgttga aaaattagga
    33001 ttggcataaa atggttgaat cgtatagtaa gaatgctaac cataacatgc gtcgtcctgt
    33061 cgtcaaagaa gaaattgtag acttgatgcg tcagcgtcaa aagcaggtca caggtttctt
    33121 gaaagaattg gaaaactttg cccgcaagga aaatattcct attattcccc atgaaacggt
    33181 tgcttatttc cgttttctta tggaaaccat gcagcctaaa aatattctgg aaattgggac
    33241 ggctatcggt ttttcagctc tcttgatggc tgaacatgcg ccaaatgcta agattacaac
    33301 tattgatcgt aatccagaaa tgattggttt tgccaaggaa aattttgccc agtttgacag
    33361 tcgcaagcaa atcactctcc tagagggaga tgcggtggat gtcttatcta cactgacaga
    33421 gtcttatgat ttcgtcttta tggattctgc caagtctaaa tacatcgtct ttctgccaga
    33481 aatcctcaaa catttggaag ttggtggtgt ggttgtcttg gatgatattt ttcaaggtgg
    33541 tgatgttgcc aaggatatta tggaagtccg tcgtggtcag cgaaccattt atcgaggcct
    33601 tcaaaaatta tttgatgcaa ccttagacaa tccagaactc accgcaacat tagtgccttt
    33661 aggagatggt attctcatgc ttcgtaaaaa tgtagcagat gttcaactgt ctgaaagcga
    33721 atgattttca gaaaaattta agaaaaaata gtaaaataga tagagtaaca cttatctcaa
    33781 aggagtagac atgaagaaaa aattattggc aggtgccatc acactattat cagtagcaac
    33841 tttagcagct tgttcgaaag ggtcagaagg ggcagacctt atcagcatga aaggggatgt
    33901 catcacagaa catcaatttt atgagcaagt gaaaaacaac ccttcagccc aacaagtctt
    33961 gttaaatatg accatccaaa aagtttttga aaaacaatat ggctcagagc ttgatgataa
    34021 agaggttgat gatactattg ccgaagaaaa aaaacaatat ggcgaaaact accaacgtgt
    34081 cttgtcacaa gcaggtatga ctcttgaaac acgtaaagct caaattcgta caagtaaatt
    34141 agttgagttg gcagttaaga aggtagcaga agctgaattg acagatgaag cctataagaa
    34201 agcctttgat gagtacactc cagatgtaac ggctcaaatc atccgtctta ataatgaaga
    34261 taaggccaaa gaagttctcg aaaaagccaa ggcagaaggt gctgattttg ctcaattagc
    34321 caaagataat tcaactgatg aaaaaacaaa agaaaatggt ggagaaatta cctttgattc
    34381 tgcttcaaca gaagtacctg agcaagtcaa aaaagccgct ttcgctttag atgtggatgg
    34441 tgtttctgat gtgattacag caactggcac acaagcctac agtagccaat attacattgt
    34501 aaaactcact aagaaaacag aaaaatcatc taatattgat gactacaaag aaaaattaaa
    34561 aactgttatc ttgactcaaa aacaaaatga ttcaacattt gttcaaagca ttatcggaaa
    34621 agaattgcaa gcagccaata tcaaggttaa ggaccaagcc ttccaaaata tctttaccca
    34681 atatatcggt ggtggagatt caagctcaag cagtagtaca tcaaacgaat agtccaaatc
    34741 aatgagtcag ggaaaaaaac tcgacttcag gaaaaaatga agcaaacatt cccacaataa
    34801 aacgcatagt acaaggtttg tactgacccc aaaagttaga caattaattt atctgaagga
    34861 tttagttctg tattgcacag ggctaagtcc ttttagtttt accttaattc tcttattgtt
    34921 gtaataatca atatagtcta taatggcttg ttccaattga ttaagtgatt taaatgtttt
    34981 ctcatagcca taaaacattt cggattttaa aatgccaaag aaagattcca tcctaccgtt
    35041 gtcttggctg tttcctttgc gtgacataga tgcttgaatt cccttactct ctaggaagcg
    35101 atgataagaa tcgtgttggt attgccagcc ttggtcacta tggagaatcg tattctcgta
    35161 gtgcttctct ttgaatgcct gttccaaca
//
LOCUS       DAVBCN010000012        34347 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVBCN010000012 DAVBCN010000000
VERSION     DAVBCN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34347
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.012"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..4786
                     /locus_tag="VQ497_000568"
     CDS             <1..4786
                     /locus_tag="VQ497_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2516919.1"
                     /translation="QGERGLTGAKGEKGDQGERGLTGAQGEKGDRGERGLTGAKGEKG
                     AQGQAGRDGVTPTVTVKDNKNDGTHTITINDGRGNVTSTVVRDGFDGASPLVATQRND
                     ADKTTTVIFYYDKNGNNELDASDKKLKEVVIADGAKGEKGDKGEQGLQGRDGEQGPKG
                     EDGKTPTVKVTDGQDGTHTITINDGKGGITTTVVRDGFDGASPLVSTHRNEADKTTTV
                     IFYYDLNDNNQFDEGDTKLKEVVIADGKQGPKGDKGDNGKDGFTPEVTVTDNNNGTHT
                     ITITQPDNRPSLTTIVKNGEDGKTPKVKAERDDAKKQTTLTFYIDKDGDGSYTAGKDE
                     LVQTTVVKDGQDGAAGASGRDGKEVLNGKVDPTTEGKDGDTFVNTQTGDVFVKKGNTW
                     EPAGNIKGPKGDKGADGAKGEKGAQGERGLTGAQGVKGEKGDQGERGLTGSKGEKGDQ
                     GERGLTGAQGAKGDKGEQGLQGRDGAQGPKGADGQRGPAGPQGPKGEQGNPGTPGKDG
                     KSLIAVKNGTETKVYVEDPARPGQPLNPNQPLATITDGRNGTNGQSPTITATRSVQNG
                     KNGVLVTITPVGGRPQTTFVEDGQKGADGKTPTVTITEGQNGTHTLTVHNPGSPDVTT
                     TIRDGATGQAGRDGKDVLNGKVNPQPNQGKNGDKYINTETGDVYVKNNGNWDKEGNIK
                     GPKGDKGADGAKGEKGDQGERGLTGAQGAKGADGAVGRDGRDGKDVLNGKANPEAHQG
                     KDGDKYVNTETGDVFVKNNGNWDKEGNIKGPKGDKGERGEDGKTPEVTVTPGKDGHST
                     DITFTVPGKDPVTVNVKDGENGLNGKTPKVDLLRVQGKNGNPSHTIVTFYTDENNDGK
                     YTPGTDELLGSEMIKDGAKGADGRDGKSLLTVKDGKETKVYQEDPANPGQPLNPEKPL
                     AVIRDGVDGKSPTVTAVRKDEAGHKGVEITVDNHDGSQPTTVFVQDGAKGETGATGQD
                     GQTPTITTQRGQDGQSTVVTITTSGKDPVTFTVKDGKNGKDGRAPKIKVEDITSPSRI
                     RRDTDAAATPTRNGIRVTVYDDVNDNGVYDEGVDKVLNSKDIYNGIDGRDGSAPTITT
                     KDNGDGTHTITVQNPDGSESTTVVKDGKDGKTANITTTENPDGSHTITVTNPDGSTKE
                     TVVKNGKDGKTPKVEVTDNNDGTHTVKVTDGDGNVTNAIIKDGKDGKAATATTTENPD
                     GSHTVTITNPDGTKNEFVVKNGRDGVDGRTPTASVRDNGDGSHTIVITNPEGVTTETT
                     VRDGKSPKVTITDEQNGTHKISVLNGDGTTTTTTVRDGREPKLEVIDNNDGSHTIKVT
                     GADGKGTTTTIFDGKSPKANIVDNGDGTHTLTIVDSDGREYKSIIKDGKDGKDSVSPT
                     VTVKNNNDGTHVVTITNPDGSKTEMVIKDGKDGKSPKVSVEDNGDGSHTITIINSDGT
                     VTKTVIKDGRDGRDGRDGKDGKDGKDGKCGCQDKPVTPSNDKPVPPTPNVPTPEVPVK
                     PVPAQPTPNVPTPEVPVQPTPAVPTPEVPVKPVPAVPEQPVVPTPAQPATPVNANPVA
                     PTTGKENRGDKLPETGSQSDYISVLLGSGILLSLYVGRRKEA"
     gene            complement(5023..5571)
                     /locus_tag="VQ497_000569"
     CDS             complement(5023..5571)
                     /locus_tag="VQ497_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2516920.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            5892..6380
                     /locus_tag="VQ497_000570"
     CDS             5892..6380
                     /locus_tag="VQ497_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HET2516921.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            6377..7564
                     /locus_tag="VQ497_000571"
     CDS             6377..7564
                     /locus_tag="VQ497_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2516922.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTVILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            7557..7922
                     /locus_tag="VQ497_000572"
     CDS             7557..7922
                     /locus_tag="VQ497_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2516923.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGAPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            7938..9284
                     /gene="asnS"
                     /locus_tag="VQ497_000573"
     CDS             7938..9284
                     /gene="asnS"
                     /locus_tag="VQ497_000573"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET2516924.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            9437..9727
                     /locus_tag="VQ497_000574"
     CDS             9437..9727
                     /locus_tag="VQ497_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET2516925.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            9739..10209
                     /gene="ssbA"
                     /locus_tag="VQ497_000575"
     CDS             9739..10209
                     /gene="ssbA"
                     /locus_tag="VQ497_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HET2516926.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            10241..10480
                     /locus_tag="VQ497_000576"
     CDS             10241..10480
                     /locus_tag="VQ497_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET2516927.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            10775..12175
                     /locus_tag="VQ497_000577"
     CDS             10775..12175
                     /locus_tag="VQ497_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HET2516928.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSYVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDVIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            12177..12536
                     /locus_tag="VQ497_000578"
     CDS             12177..12536
                     /locus_tag="VQ497_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET2516929.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            12590..13339
                     /locus_tag="VQ497_000579"
     CDS             12590..13339
                     /locus_tag="VQ497_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HET2516930.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            13329..13628
                     /locus_tag="VQ497_000580"
     CDS             13329..13628
                     /locus_tag="VQ497_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET2516931.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALFKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            13699..14634
                     /locus_tag="VQ497_000581"
     CDS             13699..14634
                     /locus_tag="VQ497_000581"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HET2516932.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVLPQLTESFNA"
     gene            14721..15233
                     /locus_tag="VQ497_000582"
     CDS             14721..15233
                     /locus_tag="VQ497_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET2516933.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGAVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            15270..15865
                     /locus_tag="VQ497_000583"
                     /pseudo
     CDS             15270..15865
                     /locus_tag="VQ497_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            15966..16160
                     /locus_tag="VQ497_000584"
     CDS             15966..16160
                     /locus_tag="VQ497_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET2516934.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(16206..17651)
                     /locus_tag="VQ497_000585"
     CDS             complement(16206..17651)
                     /locus_tag="VQ497_000585"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HET2516935.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVHRVYGLTFDVGVFLNITPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            17757..19379
                     /locus_tag="VQ497_000586"
     CDS             17757..19379
                     /locus_tag="VQ497_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET2516936.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            19583..21541
                     /locus_tag="VQ497_000587"
     CDS             19583..21541
                     /locus_tag="VQ497_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2516937.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGFLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFATAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            21713..22648
                     /locus_tag="VQ497_000588"
     CDS             21713..22648
                     /locus_tag="VQ497_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2516938.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGLIYAEKSFFDKSQS
                     GELTSAIVNDTSVIREFLITTFPNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMM
                     FIILLLSNISEKYSRRLQEEIGFLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERV
                     QNNSLKSDRVTSFETPFALLFIFATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLL
                     NPISNIANFVTVYSRSKGSSVALENLLAVPKEKFE"
     gene            22673..23325
                     /locus_tag="VQ497_000589"
                     /pseudo
     CDS             22673..23325
                     /locus_tag="VQ497_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            23483..24577
                     /locus_tag="VQ497_000590"
     CDS             23483..24577
                     /locus_tag="VQ497_000590"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET2516939.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            24585..25751
                     /locus_tag="VQ497_000591"
     CDS             24585..25751
                     /locus_tag="VQ497_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2516940.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEIQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            25844..28942
                     /locus_tag="VQ497_000592"
     CDS             25844..28942
                     /locus_tag="VQ497_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2516941.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDSFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLNRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQNIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            28990..29607
                     /locus_tag="VQ497_000593"
     CDS             28990..29607
                     /locus_tag="VQ497_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET2516942.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            29617..30951
                     /locus_tag="VQ497_000594"
     CDS             29617..30951
                     /locus_tag="VQ497_000594"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET2516943.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFSGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            30961..31425
                     /locus_tag="VQ497_000595"
     CDS             30961..31425
                     /locus_tag="VQ497_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2516944.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     DLDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            31422..31916
                     /locus_tag="VQ497_000596"
     CDS             31422..31916
                     /locus_tag="VQ497_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2516945.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            31997..33646
                     /gene="mltG"
                     /locus_tag="VQ497_000597"
     CDS             31997..33646
                     /gene="mltG"
                     /locus_tag="VQ497_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET2516946.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMTDSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDIIS
                     DTSRRSRREGAKPVNPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPIDA
                     NSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQKSM
                     STEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAEAFLA
                     KVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDEML
                     AAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDMPL
                     QSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIESSI
                     NQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            33717..34199
                     /gene="greA"
                     /locus_tag="VQ497_000598"
     CDS             33717..34199
                     /gene="greA"
                     /locus_tag="VQ497_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET2516947.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
BASE COUNT        10715 a         6372 c         7775 g         9485 t
ORIGIN      
        1 ccaaggtgaa cgcggtttaa ctggagctaa aggtgaaaaa ggggaccaag gtgaacgcgg
       61 tctcactgga gcccaaggtg aaaaaggaga ccgaggcgaa cgtggtttaa ctggagctaa
      121 aggtgaaaaa ggagcccaag gtcaggcagg tcgtgacggt gtaactccaa ccgtaaccgt
      181 taaagataat aaaaatgacg gcactcatac tatcactatt aacgacggta gaggtaatgt
      241 tacaagtact gttgtaagag atggtttcga tggcgcaagt ccattagttg cgactcaacg
      301 aaatgacgca gataagacaa caactgttat cttctattac gataaaaatg gaaacaatga
      361 attagatgct tctgataaga aattaaaaga agttgtaatt gcagatggtg ctaaaggtga
      421 aaaaggggac aaaggtgaac aaggtcttca agggcgtgat ggtgaacaag gaccaaaagg
      481 agaagatggg aaaaccccaa cagttaaagt aactgatggt caagatggaa cgcatacaat
      541 tacaattaac gatggtaaag gtggtataac tactacagta gtaagagatg gatttgatgg
      601 tgcaagccct cttgtttcta ctcatagaaa tgaagcagat aaaacaacaa ctgttatttt
      661 ctattatgat ctaaatgata ataatcaatt tgatgaagga gatacaaaac ttaaagaagt
      721 tgttatcgca gatggaaaac aaggaccaaa aggtgacaaa ggtgataacg gaaaagatgg
      781 attcacacca gaagtaacag ttacagataa caataacgga acacacacca tcacaatcac
      841 acaaccagac aacagaccat cattaacaac aatcgttaaa aacggtgaag atggaaaaac
      901 accaaaagtc aaagcagaac gagatgatgc gaagaaacaa acaacattaa cattctacat
      961 tgacaaagat ggagatggaa gttacacagc aggaaaagac gagttagttc aaacaacagt
     1021 agttaaagac ggacaagacg gagctgcagg agcatctgga cgtgatggaa aagaagtatt
     1081 aaacggaaaa gtagacccaa caacagaagg taaagatgga gacacattcg taaatacaca
     1141 aacaggagat gtattcgtta agaaaggtaa cacttgggaa ccagcaggaa acatcaaagg
     1201 accgaaaggt gacaaaggtg cagatggtgc caagggtgaa aaaggagccc aaggagaacg
     1261 cggcctgact ggagcgcaag gtgtcaaggg tgaaaaggga gatcagggtg aacgcggtct
     1321 aactggatct aaaggtgaaa aaggagacca aggtgaacgc ggtttaactg gagcgcaagg
     1381 tgccaaaggt gacaaaggtg aacaaggtct tcaaggtcgt gatggagctc aaggaccaaa
     1441 aggcgcagat ggacaaagag gaccagcggg accacaagga ccaaaaggag aacaaggtaa
     1501 tccaggaact ccaggtaaag atggaaaatc tctaattgct gttaaaaatg gaactgaaac
     1561 aaaagtctat gtagaggatc cggctagacc aggacaacca cttaatccaa accaaccatt
     1621 agcaactatc actgatggaa gaaacggtac taatggacaa tcaccaacaa ttacagcgac
     1681 acgaagtgtg caaaatggca aaaatggtgt attagtaaca attacaccag taggaggtcg
     1741 tccacaaaca acatttgtag aggatggaca aaagggtgct gatgggaaaa ccccaacagt
     1801 aacaataact gaggggcaaa acggcacaca tacattaacg gttcataatc caggaagtcc
     1861 agatgtgaca actacgatcc gtgatggagc tacaggacaa gcaggtcgtg atggtaaaga
     1921 tgtattaaac ggaaaagtaa atccacaacc aaaccaaggt aaaaatggag ataaatatat
     1981 taataccgaa accggtgatg tctatgttaa aaacaatgga aactgggata aagaaggcaa
     2041 catcaaaggc cctaaagggg acaaaggtgc agatggtgct aaaggcgaaa aaggagacca
     2101 aggcgaacgc ggcctaactg gagcgcaagg agctaaaggt gcggatggcg cagtaggtcg
     2161 tgatggacgt gacggtaaag acgtgttgaa cggcaaagct aacccagaag cacatcaagg
     2221 taaagacggc gataaatacg ttaatacaga aacaggcgac gtcttcgtta agaataacgg
     2281 caactgggat aaagagggca acatcaaagg ccctaagggt gacaaaggtg aacgcggaga
     2341 agatggtaag actccagaag taactgtaac tccaggtaaa gatggccata gtactgacat
     2401 tacattcact gttccaggta aagatccagt tacagtaaat gttaaggacg gagaaaatgg
     2461 tctgaacggt aaaactccaa aagttgattt acttcgtgtc caaggaaaaa acggaaatcc
     2521 atctcataca attgtgacat tctatacaga tgaaaacaat gacggcaaat atacaccagg
     2581 aactgatgaa cttctaggtt cagaaatgat taaagatggt gctaaaggcg cggacggacg
     2641 agatggtaaa tcattgctta ctgtcaagga tggtaaagaa actaaagttt accaagaaga
     2701 tccagctaac ccaggacaac cattaaatcc agaaaaacca cttgcggtaa ttagagatgg
     2761 agtagatgga aaatcaccta cagttacagc tgttcgtaaa gatgaagcag ggcataaagg
     2821 tgtagaaatc actgttgata accatgatgg ttcacaacca actacagtct ttgttcaaga
     2881 tggtgctaag ggagaaactg gtgcaaccgg tcaggatgga caaactccta caatcactac
     2941 tcaacgtgga caagatggcc aaagcactgt tgtaactatc acaacatcag gtaaagatcc
     3001 agtaaccttc actgtaaaag atggtaagaa tggtaaagat ggccgtgcac cgaaaatcaa
     3061 agtagaagat attacttcac cttcaagaat tagacgcgat acagatgctg ctgcaactcc
     3121 aacgcgtaac ggtatccgtg ttacagttta tgatgatgtt aatgacaatg gggtatacga
     3181 cgaaggtgtc gataaagtat taaatagtaa agatatttat aacggtatag atggacgtga
     3241 tggttcagct ccaactatta ctacaaaaga taatggagat ggaactcaca ctatcacagt
     3301 tcaaaaccca gatggttctg aatcaacaac agttgttaaa gatggtaaag acggtaaaac
     3361 tgcgaatatc actacaacag aaaacccaga tggaagccac acaattacgg tgacaaatcc
     3421 agatggttca actaaagaaa ctgttgttaa aaacggtaaa gacggtaaga ctcctaaagt
     3481 tgaagtaacg gataacaacg atggaactca tactgttaaa gttacagatg gagacggcaa
     3541 tgttaccaac gctatcatca aagatggtaa agacggtaaa gctgcaacag caacaactac
     3601 tgaaaatcca gatggaagcc acacagtaac aatcactaac ccagacggaa ctaagaatga
     3661 gtttgttgtt aagaatggac gtgacggtgt tgacggacgt actccaaccg catctgttcg
     3721 tgataatgga gacggaagtc atacaatcgt tattacaaat ccagaaggtg tgacaactga
     3781 aaccacagtt cgtgatggta aatcaccaaa agtgactata actgatgaac aaaatggaac
     3841 tcataagatc tctgttctaa atggtgacgg aacaactaca acaactacag ttcgtgatgg
     3901 tagagaacca aaacttgaag ttattgataa caacgatggt tcacacacta ttaaagtgac
     3961 aggtgctgat ggtaaaggaa cgacaactac aatctttgat ggtaaatcac caaaagcgaa
     4021 catcgttgat aacggagatg gaactcatac attaacaatc gtagattctg atggtcgtga
     4081 atacaaatct attatcaaag atggtaaaga cggcaaagat agcgtttcac caactgtaac
     4141 tgttaaaaat aataacgatg gaactcacgt tgttacaatc actaatccag atggaagtaa
     4201 gacagaaatg gtgattaaag acggtaaaga tggtaaatca ccaaaagttt ctgttgaaga
     4261 taatggtgat ggtagtcata caatcacaat catcaattct gatggaactg tgacaaaaac
     4321 agttattaaa gatggtagag atggacgtga tggtcgagac ggcaaagacg gtaaagacgg
     4381 taaagatgga aaatgtggat gccaagacaa accagtaaca ccatcaaatg acaaaccagt
     4441 tcctccaaca ccaaatgtgc cgacaccaga agtaccggtt aaacctgtgc cagcgcaacc
     4501 aacaccaaat gtaccgacac cagaagtgcc agtacaacca actccagctg ttccaacacc
     4561 agaagtaccg gttaaaccag taccagcggt tccagaacaa ccagtagtac caacaccggc
     4621 tcaaccagca actccagtaa atgctaaccc agtagcacca actacaggta aagaaaaccg
     4681 tggggacaaa ttacctgaaa ctggaagcca atctgattat atctctgttc ttttaggtag
     4741 cggtattcta ttgagcctat atgtaggacg aagaaaagaa gcttaatcag ccaaaagcag
     4801 tcactaattt ctcgaaagaa gatgataagt gatagatctc gaatgaaaga atattcttag
     4861 aatgattttc ctatgatgga aaagactgct aaaaaccttg agtggtaacg ctcaaggttt
     4921 ttttggatgg ctgatgtttg tctgaaaatc ttgattgaga aataaaaagc caccctatta
     4981 ggtggctata attgatttag actttctaaa attttctatt ttttactgaa tcgcttcgac
     5041 caagtcttga gcttgttttt caagtgagtt taggactgtt tcttcaagaa ccaattttcc
     5101 gtctgcccag gcagagtcat taacacgtgc agcagtgaaa tcaccaacgc cttgtgtacg
     5161 gataaatggc aagaggtctt tgtagatagc gaaaagttga tcgtgccctg cattggctac
     5221 agatgatacg gtgacaaact tgtcttgaag ggcagaaacg ccacgcgtat cagacaagtc
     5281 aagggcacga gatagccagt caagcaagtt ttttactgta ccagggatag agaagttgta
     5341 gactggagag aaaatccaga tagcatccgc aacgagaact gcttcacgag cagcagctac
     5401 agctggatgt gttggaactt ccaaatcttg gctgaagaga ggaagggctg aataatcaag
     5461 gtagctaact tccgctttac cagcaagtgc tttctcagct tcgagcgcca tttggtggtt
     5521 gaaagaacct tgacgtagtg atccgacgat aaataatact tttttagaca tgatgtgtct
     5581 cctttttctt caaattttga gaactctctc gttgttataa aatacattac aacaaaacaa
     5641 aagaacttgc aagaaatttg ctcagagttt atttgaaaat tgtaaaattt cggaaactct
     5701 agtcatttgt gactgaaaaa gtaagaactg tttcactttc aaaatcggtt ttgtttgaag
     5761 ggagaaatag aattggttgc tcaaagcaca gctttgaggt tgcagataaa gctgacgtgg
     5821 tttgaagaga ttttcgaaga gtattcttat attcacttgt aataaagctt aatagatggt
     5881 aaaatagacg agtgaaaaaa agacaaaaca aagcaaaaaa taatctactg tggcagtatg
     5941 gtctagggat gacgattttg tttgtggtta tcagtgcttc ctttctgtat atggtttctc
     6001 ttagcatgaa accctatcaa acagctaaaa gtgaaggaga aaaattagct cagcagtatg
     6061 caggattaga gcaggccgat caggttgatt tatacaatgg cttggaatct tattacagcg
     6121 ttcttggtcg taataaacag caagaagcac ttgctgttct gattggaaaa gatgatcata
     6181 agatttacgt ttatcagcta aatcagggtg tttcacaaga aaaagcagaa acggtttcta
     6241 aggaaaaggg agctggcgaa attgacaaga ttatctttgg tcgttatcaa gataagccaa
     6301 tctgggaagt caagtcagga tctgattttt atctagtaga ttttgaaaca ggagcattgg
     6361 tcaacaagga gggcctatga aattatccaa ccgtgtttta gaaatggaag aaagtgtgac
     6421 tttggctgct ggagcccgtg ccaaagcact gaaggctgag ggtagagata ttttgtctct
     6481 aaccttgggt gagccagatt tcactactcc caaaaatatc caagatgccg ccattgcatc
     6541 gattcgagat gggcgtgctt ctttttatac agtaacctca ggtctgccag agcttaaggc
     6601 ggcggtcaat agctactttg agcgctttta cggctattct gtagcgtcaa atcaagtgac
     6661 agtcgctgcg ggagccaaat attctctcta taccttcttt atggctgtgg tcaatccagg
     6721 tgatgaagtc atcatcccaa ccccatactg ggttagctat ggagatcagg tcaagatggc
     6781 agaaggcgtt cccgtctttg tttctgctaa ggaagacaat cactttaagg tgaccgtaga
     6841 gcagttagaa gcagctcgca ctgacaagac caaggttttg gtgctgaatt cgccatctaa
     6901 tcccacaggt atgatttaca cccgtgagga actcttggca attggaaact gggctgtaga
     6961 aaatgatatt ctcatcctag cagatgatat ctatggccgc ttggtttata atggtcatga
     7021 gttcacaccg atttctagcc tatcggaagc gattcgcaag caaacagtgg tcatcaatgg
     7081 tgtgtctaaa acctatgcca tgactggttg gcggattggt tatgccgttg gagaagcaga
     7141 cattatcgct gccatgtcca agattgcagg tcaaacaact tcgaatccgt cagcagtagc
     7201 ccaatatgca gcagttgagg ctctatcagg tgagcaagat actgtagaaa gcatgcgtca
     7261 ggcctttgaa gaacgtctta ataccatcta tcccctcctt gcagaagtgc caggatttga
     7321 agtggtcaaa ccgcaagggg ccttctatct cttcccaaat gtcaaaaagg ccatggagat
     7381 gaaaggctac acggatgtga cagactttac aactgttatc ttagaagaag ccgaagtggc
     7441 cttggtaaca ggagctggat ttggagcacc agaaaatgtc cgcctcagct atgcgacaga
     7501 cctagacacg cttaaagaag cagtcgaacg cttgaaagca tttatgggta gtgagaatga
     7561 ttgatcattt tgagattaag gtaaaggatt tacaaatttc agaaggattt tataggagtt
     7621 ttctcgctcc tttggactat aaattggctt ttaagactag ttctctaatt agttttctat
     7681 ccccgaacag ccctcatcct ggtggtgatt tttggctgac tcagggaaca caagatcctg
     7741 ttcattttgc ttttttagca gaaaataagg aagaagttca ggcttgttat gaggctggct
     7801 tagaggcagg tgggcgagac aatggggctc ctggttatcg aagtgagcat ccgatttact
     7861 atgctgcttt tatgattgac ctggatggga acaatataga ggtggtttgc cataaagaat
     7921 aaaaaagaaa aggaaaaata atgacaaaac gtgtaacgat tattgacgta aaagactatg
     7981 ttggtcagga agtgacgatt ggcgcttggg ttgccaacaa atcaggaaaa gggaaaatcg
     8041 ctttcttaca attgcgtgat ggaacagcct tctttcaagg tgtggctttt aaaccaaact
     8101 ttgtcgaaaa atttggtgaa gaagtgggac ttgagaagtt tgatgttatc aaacgcttga
     8161 gccaagaaac gtctgtttat gtgacaggta ttgtcaaaga ggacgaacgt tctaaatttg
     8221 gctatgagtt ggacatcaca gacatcgaag tgatcggtga atctcaagac tacccaatca
     8281 caccaaaaga acacggaaca gactttttga tggataaccg tcacttgtgg ctacgctctc
     8341 gtaagcaagt agctgtgttg caaatccgta acgctattat ctatgcaact tatgagttct
     8401 ttgacaagaa cggttttatg aagtttgaca gcccaattct ttcaggaaat gcggcagaag
     8461 attctacaga actctttgaa actgactact tcggaacgcc agcctacttg agccaatcag
     8521 gtcaacttta cctagaagca ggggctatgg ctcttggtcg tgtctttgac tttggtccag
     8581 ttttccgtgc tgaaaaatca aaaacacgcc gtcacttgac tgagttctgg atgatggatg
     8641 ctgagtactc atacttgaca catgatgagt cgcttgactt gcaagaagct tatgtgaaag
     8701 ctcttctaca aggtgttctt gaccgcgcgc ctcaagcctt ggaaaccttg gaacgtgata
     8761 cagaactctt gaaacgctac attgcagagc cattcaaacg tatcacttac gatcaagcca
     8821 ttgacctctt gcaagagcat gaaaatgatg aagatgctga ctacgagcat cttgagcatg
     8881 gtgatgactt tgggtcacca cacgaaactt ggatttcaaa ccactttggt gtgccaacat
     8941 ttgtcatgaa ctatccagca gccatcaagg ccttctacat gaaaccagtt cctggaaatc
     9001 cagagcgcgt gctttgtgca gacttgcttg ctccagaagg ctatggagaa attatcggtg
     9061 ggtctatgcg tgaggaagat tacgatgccc ttgtcgctaa gatggatgaa cttggcatgg
     9121 atcgtacaga atatgaattc taccttgacc ttcgtaaata cggtacagtt ccacacggag
     9181 gatttggtat cggtatcgaa cgtatggtaa ccttcgcagc aggaacaaaa catatccgtg
     9241 aagctattcc attcccacgt atgttgcacc gtatcaaacc ataagaacag gaaaacgccc
     9301 atgtggcgtt ttttcgaatt ctagtgtttt tacttgcgta aaaaaaattt ttctagtata
     9361 atagtttatt gtgagcgaac ctcacttacc ccttgcaaag tcttggggtc attagaccaa
     9421 aaggaggaac atatcaatgg ctaaatacga aattctttat atcattcgtc caaacattga
     9481 agaagaagct aaaaacgctt tggtagcacg ttttgactct attttgactg acaacggtgc
     9541 aactgttgtt gaatcaaaaa cttgggaaaa acgtcgtctt gcatacgaaa tccaagattt
     9601 ccgtgaagga ctttaccaca tcgttaacgt tgaagcaaat gacgatgcag ctcttaaaga
     9661 gtttgaccgt ctttcaaaaa tcaacgctga cattcttcgt cacatgatcg tcaaaattga
     9721 cgcgtaagaa ggtaaactat gattaacaat gttgtacttg tagggcgtat gacacgtgac
     9781 gctgaattgc gttatacccc atcaaatgta gcagttgcga cttttactct tgcagtaaac
     9841 cgtacattta agagtcaaaa tggtgaacgt gaggctgatt ttatcaatgt cgttatgtgg
     9901 cgccaacagg ctgaaaatct tgctaactgg gctaaaaaag gctcacttat cggggtgaca
     9961 ggtcgtatcc agactcgtag ttacgataac cagcaaggac aacgtgtcta cgtgacagag
    10021 gtcgtggctg agaatttcca aatgttggaa agccgtagtg tgcgtgaggg tcacacaggt
    10081 ggagcttact ctgcaccaac tgcaaactat tcagcaccta caaattcagt accagacttt
    10141 tcacgtaatg aaaatccatt tggagcaaca aacccattgg atatttcaga tgatgattta
    10201 ccattctaat ggacaactaa acttataaag gagaaaaaac atggctcaac aacgtcgtgg
    10261 cggattcaaa cgccgtaaaa aagttgatta catcgcagca aacaaaattg aatatgttga
    10321 ttacaaagat actgagcttc ttagccgttt cgtttcagaa cgtgggaaaa tccttcctcg
    10381 tcgtgtaaca ggaacttcag ctaaaaacca acgtaaagta acaacagcta tcaaacgcgc
    10441 tcgcgtaatg gctttgatgc ctttcgtaaa cgaagattaa agaaaagacc cggttgggtc
    10501 tctttttcat gaacttgaaa acaagaaagc gaattaagac cccaacgggt cttttttcac
    10561 aagctttgaa atgagagagc gagttaggct cagtggctcc ctttttacga gtttgaaaac
    10621 aagagaacac attaaaagca cgatggagct ttttcataaa tatgtctttt ttttcatcca
    10681 agaaattaaa ataaaaatga taaacttttt gaaaataggg gatttgccta ctttatgata
    10741 taatggaaag agttagattg aaagaggtag tgagatggac gcgaaattaa gatacaaggc
    10801 aaagaagatc aagattgtct tttttgatat tgatgataca ttgcggaatt caaagacagg
    10861 gtttattcca actacaattc ccactgtttt taaacagttg cgtgaaaaag gaattttaac
    10921 aggaatcgcc tctggacgtg gcatttttgg tgttgttcca gagattcgtg atctcaaacc
    10981 tgactttttt gtaactttga atggggctta tatcgaagat aaaaaaggtc aggtcattta
    11041 tcagcatcag attgaaaagt catatgttga ggagtatatc tcttgggcta agcaagaagg
    11101 aattgagtat ggcttggttg ggagtcatga tgccaagttg tcgactcgca ccgatatgat
    11161 gagtgaagct atcaatccaa tttatcccga cttagatgta gatcccgatt tccatgaaaa
    11221 agaagatatc tatcagatgt ggacttttga agataaggga gatgacttgc acttgcctga
    11281 cagtctctca gacaaacttc gcatggttcg ttggcatcaa cattcgtctg atattgtgcc
    11341 gatttcaggc tccaaagcga cgggggtgga aaaggttgtg gaacaccttg gcttgaaacc
    11401 agagaaggtc atggtttttg gagatggtct caatgacttg gaactctttg attatgctgg
    11461 aatcagcgtt gccatgggaa tttctcatga taaaatcaaa gaaaaagcag attatattac
    11521 aaaaacatta gaagaagatg gcatttttga tgccttagaa gtatttggta tggtagaaaa
    11581 agaattgcat tttccacaag tagacattga aacagtagaa ggtcctcttg cgactattaa
    11641 gaccaatcac ggagacttac gtatcaagct tttccctgaa catgctccta aaacagtggc
    11701 taactttgta tctctttcaa aagatggcta ctatgatggt gtcattttcc accgtattat
    11761 caaggacttt atgatccaag gtggagaccc aactggaact ggtatgggtg gcgagtcaat
    11821 ctacggcgaa tcttttgagg atgaattctc agaagagctt tacaatatcc gtggtgctct
    11881 ttccatggca aatgctggtc caaataccaa cggcagccag ttctttatcg tgcaaaacca
    11941 acacctacct tattctaaga aagaaattac tcgtggtggt tggccagaac cgattgcaga
    12001 aatctatgcc aatcaaggtg ggacacctca cctagaccgc cgtcacacgg tttttggtca
    12061 gttagctgat gaagcatctt acgctgtctt ggatgtcatt gctgctgttg agacaggagc
    12121 tatggacaag ccagttgaag atgttgtaat tgaaactatt gaaatcgagg actaagatga
    12181 aaatcggtga taagctaaag gggcgtatta cagggattca gccctacggt gcctttgttg
    12241 agttagagac gggtgatacg gggctgattc acatttcaga aattcggaca ggatttattg
    12301 aaaatattca tgaaaccttg aaagtcgatg aagaggttca agttcaggta gtggatttag
    12361 atgaatttac ggggaaagcc agtctttcta tccgcacttt ggaggaagaa aagtaccagt
    12421 ttcctagacg gagacgcttt tcaagcgacc gctttaacta cggttttgct ccctttcgac
    12481 gaatgctacc aatctggact ggagaagccc tgcaccattt aaaaaagaag aagtagttta
    12541 gagttttata ttttttgtaa tgttaatata aatttgctat aataggacca tggaagaaga
    12601 acaattatta aaatcaggag agcgcattaa ccagctcttt tcgacagata tcaaaatcat
    12661 tcaaaataga gaggttttta gctattcggt ggatagtgtt ctcttatcac gatttccacg
    12721 ttttcctaag aaggggttga ttgtggattt ctgtgctggg aatggagcag tggggctttt
    12781 tgctagcact cgtactcaag cacagatatt gtctgttgag attcaggagc gtttggcgga
    12841 tatggctgaa cgctctgtcc gtttgaatgg attagaggag caaatgcagg tcatctgcga
    12901 tgatttgaaa aatatgcctg ctcacattca ggggagtaag gtggatatga ttttgtgtaa
    12961 tccaccctat ttcaaggtgg atccttattc taatctgaac gagagtgaac attatctctt
    13021 ggctcgacac gaaatcacga ctaatttgga agaaatctgt catagtgccc agagtattct
    13081 caagtctaat gggcgtttgg ccatggttca tcgtcctgat cgacttctgg atattttgga
    13141 tacgttaaaa cgacataatc tagcccctaa gcgcctgcag tttgtttatc caaaaagaga
    13201 aaaggaagcc aatatgcttt tgattgaggc gatcaaggat ggctcaacaa gtggctttaa
    13261 ggtcttacct cctttgattg tccacaatga tgatggctct tatacgcccg aaatcgaaga
    13321 gatttattat ggatcataag gcttatatgt acgttctgga gtgtcgtgat ggatcctact
    13381 atataggcta tacgactgat atgagaagac gcctcgctat ccacaatagt gggaagggag
    13441 ccaaatatac acgagcacgc ttgccagtca aacttatcta tgctcaaggt tttgccagta
    13501 aggaagaagc catgtcggct gaagctcttt tcaagcgtaa gaagaggcca cagaaggaag
    13561 aatttttatc tgaaaatcaa gatagaaatt tactcagtta ttttgaagaa tcgtggggag
    13621 tcctttgact cctcttacct ttgtcaaaaa gcgaaaaaaa tgctacaata aagaaaataa
    13681 acaaaatgag gtattatcat gtccaagatt ctagtatttg gtcaccaaaa tccagactca
    13741 gatgccatcg ggtcatctgt agcttttgcc taccttgcaa aagaagctta cggtttggat
    13801 acggaagctg ttgcccttgg aactccaaat gaagaaacag cctttgtctt gaactatttt
    13861 ggtgtggaag caccacgtgt tatcacttct gccaaagcag agggggcaga gcaagttatc
    13921 ttgactgacc acaatgaatt ccaacaatct gtatcagata tcgctgaagt agaagtttac
    13981 ggtgttgtag accaccaccg tgtggctaac tttgaaactg caagcccact ttacatgcgt
    14041 ttggagccag ttggatcagc gtcttcaatc gtttaccgta tgttcaaaga acatggtgta
    14101 gctgtgccta aagagattgc aggtttgatg ctttcaggtt tgatttcaga tacccttctt
    14161 ttgaaatcac caacaacaca cccaacagat aaaatcattg ctcctgaatt ggctgaattg
    14221 gctggtgtaa acttggaaga atatggtttg gcaatgttga aagctggtac caacttggct
    14281 agcaaatctg ctgaagaatt gattgacatc gatgctaaga cttttgaact caacggaaat
    14341 aatgtccgtg ttgcccaagt gaacacagtt gacatcgctg aagttttgga acgccaagca
    14401 gaaattgaag ctgcaatgca agctgccaac gaatcaaacg gctactctga ctttgtcttg
    14461 atgattacag atatcgtcaa ctcaaactca gaaatattgg ctcttggtgc caatatggac
    14521 aaggtcgaag cggctttcaa tttcaaactt gaaaacaatc atgccttcct tgctggtgcc
    14581 gtttcacgta agaaacaagt gctacctcaa ttaactgaaa gctttaatgc gtaagatttt
    14641 gggtgtcagc tcaaaatcgg aaagtctagt ttgccttata tcgaaaggag tttcggctcc
    14701 tttttttcta ggagtgaagt atgttagaaa atggcgattt gatttttgtg agagatgggt
    14761 cagacatggg acaggccatc cagacttcca caggtaacta tagccatgtt gccatttatt
    14821 tggatgggat gatttatcat gctagtggac aggctggtgc tgtctgtcaa gaaccggcag
    14881 acttctttga gtccaatcat ttgtacgacc tctatgttta cccagaaatg gatatccagt
    14941 cggtgaagga aagagcttgc agacatcttg gagcacccta caatgcttct ttctatccag
    15001 atgcagctgg tttctactgc tcccagtata tagcagaaat cctacctatt tttgaaacta
    15061 ttcctatgaa atttggagat ggggaacagg agattagtga tttttggagg gagtattaca
    15121 tagaactagg tctgcctgtt cctctgaacc aagctgggac caatcctagt cagttggcag
    15181 catcgcctct gttacaatgt aaagaaagga atcttcatga ttcagatttt taattcatct
    15241 cgtttgacga gacagccatt tttggagaat tgatccgcta tctggaccag tatgaggatg
    15301 tgattctacg ggaaattaag gctcaatttc cagatgttgc agttgataaa ctcatggaag
    15361 agtatataaa ggcaagcttg attctacgtg aaaataagcg ctattacctc aattttccta
    15421 cgcttgaatc acttgatagt cttgaactgg atcaagagat ttttgtcaga gaagctagtc
    15481 cggtctatca agccttgttg gagcagagtt ttgagacgga attgcgcaat caaatcaatg
    15541 cagctatttt agttgaaaga cggactttgc gcgcattaaa atgaccctgt ccaattattt
    15601 ttacaaggtc aaacagcagt atcctttgac agaaaaacag caggagctct atgacatttt
    15661 aggagatgtt aatcctgagt atgcactcaa gtatatgacg gcttttttgt tgaaatttct
    15721 caaaaaagac cagcttatgc agaaatgccg tgatatcttt gtggacagtt tggttgtctt
    15781 aggctatatt gtgcaaaatg aagatagaaa gtatgagttg gctatcgatt ttgataagga
    15841 gagattaact ttctacttag cgtgatttct tgtttctgag cacattgttt gactttcctt
    15901 agtattcggt ataaactata tgtaaccggt aacacatatc ggaataaact aaaggagaca
    15961 atcatatgtc acttgaaaac aaattggaac aagcaacagg cgctgtcaaa gaaggttttg
    16021 gtaaagttac tggagacagc aagacagaac ttgaaggagc tgttgaaaaa acagttgcta
    16081 aggcaaaaga cgttgtagaa gacgcaaaag gtgctgtaga aggtgccgtt gaaggtttga
    16141 aaaacgtttt tactaaataa taggaaaaaa tcaagggttt cattttccct tgattttttc
    16201 tattcttata aataattttc tgcgacggct gtatctcctg ggtaggattc tttcttgccc
    16261 tggatgattt ggtaacaatc ggctccctta cccgcaataa taactgcatc taattcgtga
    16321 tttgtgatag ccattgccgc cttgatggct tcttggcgat ccgcaatctt ttcaacagga
    16381 tgattgatgt agctactaat ttcatctgca atggccattg ggtcttcata gttagggtca
    16441 tcagcagtca gaaagacttg aatctcaggg tgttgattga ggaggaggcc aaagtcctta
    16501 cgacgacttt ctcccttgtt tcctgttgat cccagaacca gagcaatctt tccggtttga
    16561 tgagtttcaa ccacattgat gagttttttc agactatccc cattgtgggc atagtcgatg
    16621 aagaccttgg ctccattttt ctgagtgagg acttccatac gaccaggaac gcgggttgca
    16681 gcgatgcctt ttttgatgtc ctcaagactt gctccgagac ggagacaagc aagtccagca
    16741 gcaactgcat tttcttggtt gaagttgcca atgagttgga tatcataatc tccagcgagt
    16801 ttacccgtag ctgaaaagct aaaggctttg gaattctcga tttggttatc aaattggcta
    16861 ccatagaaat catggtcttg atcttcaacc tgttctttca agactgagaa gtggtccatg
    16921 tcactgttaa tgatgactgc tcggctattt tccatcaaga gacgcttgtg gtagaaatag
    16981 tcttcaaagc tagggtgttc aatcgggccg atatggtcag gagtgatgtt aagaaagact
    17041 cctacatcaa aggtcagtcc atagactcga tggactagat aggcttgact ggagacttcc
    17101 atgatgaggt gggtacggtc attttgcaca gcctgattca tcatgtcaaa gaggtcaata
    17161 ctctcagggg ttgtcaacgc tgacttaaag aaagtctcgc catcaagagt tgtgttcatg
    17221 gtcgacaaca tagcaggtct atgcccttga gataagatgt tataggcgaa ataggttgct
    17281 gttgtcttac ccttagtacc agtaaaggca aggagtttga gtttctcttg tggattacca
    17341 tagaactcca tggcaatcaa actcatggct ttctttatat cgttcacaat gatgacaggg
    17401 atatcgactt cgtagtcctt ttcagctaca taccaagcta aaccttgtgt tatagcagaa
    17461 agaaggtatt cttttttaaa ggcagcgcct tttgcaaaaa aaagagtgtc ttctgttact
    17521 tttcggctgt cgtagctgat gctatcaaaa ataactttgc tgtagttgta gtggtaatga
    17581 ccttggtcaa taatttcgcg aaaaaggcca tctttcttta aaatatctaa tacggtttca
    17641 atcttaatca tactttctat tgtaaaccga aagtcgtaaa tttacaagta acaaggaaaa
    17701 gtttataatg gaagataagg agtttttcct agttatcaaa attgaatgag gaatctatgt
    17761 cgcacgaaaa caatcaccag caggcccaga tgttacgggg gactgcttgg ctaacggcta
    17821 gtaactttat cagtcgccta ctcggggctg tttacattat cccttggtac atctggatgg
    17881 gggcttatgc agctaaggca aatggtctct ttaccatggg ttacaatatc tatgcttggt
    17941 tcttgttggt ttcaacagcg gggattccag ttgcggtggc caagcaagtt gccaagtata
    18001 atactatgcg agaagaagag catagctttg ccctgattcg gagcttctta ggctttatga
    18061 caggactagg cctggttttt gctttagtct tgtatgtctt tgctccttgg ctagcagact
    18121 tgtctggcgt gggcaaagac ttgatcccaa tcatgcaaag cttggcttgg ggagtcttga
    18181 ttttcccgtc tatgagtgtt atccgaggat ttttccaagg gatgaataac ctcaaaccct
    18241 atgccatgag ccaaattgct gagcaggtca ttcgtgttat ctggatgctc ctagcaacct
    18301 ttatcattat gaagctcggt tcaggagatt atctagcagc cgttacccaa tcaacctttg
    18361 ctgcctttgt cggtatggta gccagttttg cagtcttgat ttatttcctt gcccaagaag
    18421 gttcactcaa aagagtcttt gaaacaggag ataagattaa cagtaagcgt ctcttggttg
    18481 ataccattaa ggaagccatt ccttttatcc tgacagggtc tgccatccag ctcttccaga
    18541 ttttggatca gctgaccttt atcaatagta tgagctggtt taccaactac agcaatgagg
    18601 acttggttgt catgttttct tatttctcag ccaatcctaa taaaatcacg atgattttga
    18661 tttctgtagg ggtttcgatt gggagtgttg gtttgccact tttgacggaa aactatgtca
    18721 agggggactt gaaagcagct tctcgtctcg ttcaggacag tctcacccta ctctttatgt
    18781 tcttgctacc agcaacggtt ggagtggtta tggtaggaga acctctttat acggtcttct
    18841 atggtaagcc agatagtttg gctctgggct tatttgtctt tgcagttttg cagtctatta
    18901 ttttaggctt gtacatggtc ttgtctccaa tgcttcaggc catgttccgc aaccgcaagg
    18961 ccgttctcta ttttatctat ggttctattg ccaagctagt cttgcaacta cctaccatcg
    19021 ccctcttcca cagttatggt cctttgattt caacaaccat tgctctcatc attcctaacg
    19081 tcttgatgta tcgggatatt tgtaaagtaa ctggtgtcaa gcgcaaggtg attttgaagc
    19141 gaaccatttt aatcagtttg ctgaccctag tcatgtttct gttaatagga accatccagt
    19201 ggctgttagg atttttcttc caaccaagtg gacgtttgtg gagcttcttt tatgtagctc
    19261 ttgtcggtgc catggggggt ggactttata tggttatgag tctgcgtacc tatttattag
    19321 ataaggtaat aggaaaagcc caagcagatc gcctgcgagc aaaatttaag ctttcgtaag
    19381 gtagcattta taaataaaag gaataagttg aacaatcttt taggagaact tgaagcttat
    19441 tcctttttta gcgtaaaaat agctaaaatt atatttcaaa aatagaaaat gaggcttttt
    19501 tcgatttttt ggaaaaaatc attgactaaa caaactaaac gttgtataat aaaacaaata
    19561 tttaaagcag gaggttccgg aaatgaaaaa gtctaagagc aaatatctaa ccttggcagg
    19621 tcttgtcctg ggtacaggag ttttattgag cgcgtgtgga aattctagca cggcgtcaaa
    19681 aacatacaac tatgtttatt caagtgatcc atctagcttg aactatctag cagaaaaccg
    19741 cgcagcaaca tccgatattg ttgcaaattt ggtagacggg ttattagaaa atgaccaata
    19801 tgggaatatt attccatcat tagcagagga ttggactgtt tctcaggacg gtttgaccta
    19861 tacctacaaa cttcgtaagg atgccaagtg gtttacttct gagggagaag aatatgcgcc
    19921 tgtaactgcc caggattttg tgacaggttt gcaatatgca gctgataaaa aatcagaagc
    19981 cttgtatcta gtgcaggact ctgttgctgg tttggatgac tatatcactg gtaaaacaag
    20041 cgacttttca actgtcggtg tcaaggcact tgatgaccaa acggttcaat atactttggt
    20101 taaaccagaa ctttactgga attcaaaaac acttgcaacg atactttttc ctgttaatgc
    20161 agatttcctg aaatcaaaag gggatgattt tgggaaggcg gatccatcta gtattttgta
    20221 caatggacct ttcttgatga aagcacttgt ctcaaaatct gctattgaat ataagaaaaa
    20281 ccctaattac tgggatgcta agaatgtctt tgtagacgat gtgaaattga cctactatga
    20341 tggtagcgac caagaatcac tggaacgtaa ttttacagct ggtgcttata ctacggctcg
    20401 tctttttcct aacagctcca gctatgaagg gattaaagaa aaatacaaaa acaatatcat
    20461 ctatagtatg caaaattcaa cttcatattt ctttaatttt aacctagata ggaagtctta
    20521 caattatact tctaaaacaa gtgacattga aaagaaatcg actcaggaag cagttctcaa
    20581 taaaaacttc cgtcaggcta tcaattttgc ttttgacaga acatcttatg gggctcagtc
    20641 tgaagggaaa gaaggtgcaa caaagatttt gcgtaaccta gtggttcctc caaactttgt
    20701 cagtatcaag ggaaaagact ttggtgaagt tgtagcctct aagatggtca actatggtaa
    20761 ggaatggcaa ggtatcaact ttgcggatgg tcaagaccct tactacaatc ctgagaaagc
    20821 caaggctaag tttgcggaag ctaagaaaga actcgaagca aagggtgttc aattcccaat
    20881 ccacttggat aagactgtgg aagtaacaga taaagtaggc atacaaggag ttagttctat
    20941 caaacaatca attgaatctg ttttaggttc tgataatgta gtgattgaca ttcagcaatt
    21001 aacatcagat gagtttgaca gttcaggcta ctttgctcaa acagctgctc agaaagacta
    21061 tgatttatat catggcggtt ggggacctga ttatcaagac ccgtcaacct atctcgatat
    21121 ttttaatact aatagtggag gatttctgca aaatcttgga ctagagcctg gtgaggccaa
    21181 tgacaaggct aaggcagttg gactggatgt ctatactcaa atgttggaag aagctaataa
    21241 agagcaagat ccagccaaac gttatgagaa atatgctgat attcaagctt ggttgattga
    21301 tagttcttta gttcttccaa gtgtttcgcg tgggggaaca ccatcattga gaagaaccgt
    21361 accatttgct actgcctatg gtttaaccgg tacaaaaggg gttgaatcat ataaatacct
    21421 caaagtacaa gataagattg tcacaacaga cgaatatgca aaagccagag aaaaatggtt
    21481 gaaagaaaaa gaagaatcca ataaaaaagc ccaagaagaa ttggcaaaac atgtcaaata
    21541 aaatctaatt gtagataagt ttgtgtaaga agtctttgtg cttaaaaacg cataatatca
    21601 ggtatataaa atcctagatg ttatgcgttt tgttatagga agatttatta gattttatcc
    21661 tcaaattgag ttattcccga ctttatccgt tgaaatatta taaggagtat ttatgaaatt
    21721 aaaattatta agagtagata ctaaggtgat tatggggagt ttcttacttg ttctgtctag
    21781 tctacttgct ttgttgcttc cccttatctt aaagggttta atagatggga gttctattga
    21841 aaatataggt tccaaagtat ttcaatcgtt tttgattttt attggtcaag ccttgttttc
    21901 ttctattggt tactatctgt ttagtcaatc gggtgaaaaa aagatagcaa aaatcaggaa
    21961 aaaagtgata gaggggttga tttatgcaga gaaatccttc tttgataaga gccaaagtgg
    22021 ggagttgact tctgccattg tcaatgacac gagtgtcatt cgtgagtttt taattacgac
    22081 tttcccaaat attattctga gtttagttat ggtacttggt tccattgtag tcttatttag
    22141 tcttgattgg aatctttctc tacttttatt catcactctt ccttgtatga tgtttattat
    22201 cttgctcctt tccaatatca gtgaaaagta tagtcgtcgt ttacaggagg aaatcggatt
    22261 tttaacaggg caattgactg aaaagattca agaacatgaa ttaattaaga ctaaccaagc
    22321 agagaaaagt gtacaggatg ttttggataa ttgtattgaa agggtacaaa acaattcact
    22381 gaaatcagat agggttactt cttttgagac cccgtttgct ctcttattta tctttgcgac
    22441 tatagctgtg atgctaacct atgggggtta tcgggtcagc gcaggatata tatctgtggg
    22501 aaccttggtt tcgtttttga tttacctctt tcaattactt aatcctatta gtaatatagc
    22561 taattttgta actgtttatt ctaggagcaa gggatcttca gttgcactgg agaacttgct
    22621 tgcagttcct aaagaaaaat ttgagtgagg aaaatcggta tcaggacaag ggttgaattt
    22681 taaccatgtc tattttggtt atgatgaaaa tcgacctgtc ttaaaggata ttacttgttc
    22741 aattttcaag gggcaaaaaa ttgcttttgt tggaccatct ggatcaggaa aatcaacgat
    22801 tgtgcgtttg ttagagcggt tttataaacc gctttcagga gatattctaa tggagcaatc
    22861 aagtatatat gattttaact taaaagaatg gagaagtaaa atcgcttagt aactgatgat
    22921 gaattgatga aagtgctaga cttagtatca ctaggtgatg agattcgctc catgaaagag
    22981 ggactagata ctgaagttgg tgaacgcgga cgactcttgt caggggggca aagccaaaga
    23041 cttcaaatag ctagagccta cttaaaagat gctgaaattc ttatatttga tgaagccact
    23101 gctaatcttg atgcggattc tgagtatgcg attatcagta gcctctattc tgtattaaag
    23161 gagaagacgg ttgtgattat agcgcatcgt ttgtcaacgg taaaagatgt ggattgtatt
    23221 ttcttcttag aggaggggaa aatcactggc tcaggaactc ataaggaact actggaaaat
    23281 catgagcgtt atgctcgttt tgtgcaggag caaatgatag agtgaaggtg tcttttgaga
    23341 ttcaccattt tatagtctat taaagggagc aggaaaaact ccctttttat atagtttgaa
    23401 actataacta gctcttgaaa agaagaaaat gagttgatga aaataagtgg tacaatagtt
    23461 actatagatt tggaggtatt gtatgagcaa ggaattacac attaacacaa ttttggccca
    23521 ggcgggtatt aagtcagatg aagcgacagg tgcattggtg acaccgcttc atttttcaac
    23581 gacctatcag catccagagt ttggtcgatc tactgggttt gactatacgc gcactaaaaa
    23641 tccaactcgt agtaaggctg aggaagtctt ggcggctatt gagtcagcag actatgccct
    23701 agcgactagc tcagggatgt cagctattgt actggccttt agcgtctttc cagtaggaag
    23761 taaggtcttg gcagtgcgtg atctttacgg tggttctttt cgctggttta accaagtgga
    23821 gcaggaaggt cgtttccatt ttacctatgc caatacagaa gaagagttga ttgccgagtt
    23881 agaaaaggat gtggatgttc tctatatcga aaccccaacc aatcccttga tgttggaatt
    23941 tgatatcgaa aaactagcaa aattggctca tgctaagggt gccaaagtgg tggtggacaa
    24001 taccttctat agccctatct accaacgtcc gattgaagat ggagcagata tcgttctcca
    24061 ttcagcaacc aagtatctag caggccacaa tgatgtcttg gctggagtgg ttgtgaccaa
    24121 tagtttagaa ctatacgaga agctttttta caatctcaat acaacagggg cagtcttgtc
    24181 tccatttgac agctaccagt tgcttcgtgg tctcaagacc ttatctcttc gtatggagcg
    24241 ctcaacagct aacgcccaag aagtggttgc ctttttgaag gattctccag cagttaagga
    24301 agttctctac actggtcgtg gaggcatgat ttcctttaaa gtagccgatg aaacacgcat
    24361 tcctcatatt ttgaacagtc tcaaggtctt ctcttttgcg gaaagtttgg gcggagtgga
    24421 aagtcttatt acttatccaa cgactcaaac tcatgctgat attccagcag aagtacgcca
    24481 ttcttatggt ttgacagatg acctcttgcg tttgtctatt gggattgagg atgctagaga
    24541 tttgattgca gatttgcgcc aagccttaga aggataagac aaagatggga aaatatgatt
    24601 ttacaagcct gcccaaccgt ttagggcacc atacctataa atggaaagaa acagaaacgg
    24661 atagtgaagt tctaccagct tggatagcgg atatggactt tgtggtcttg cctgaaatcc
    24721 gccaagccgt gcaaacttac gcagaccaac tggtttatgg ttatacctat gccagtgaag
    24781 acttaattaa ggaaattcaa aagtgggaag ctacacaata cggttacaac tttgacaaag
    24841 aggctcttgt ctttatcgag ggtgtggtac cagccatctc aacagctatt caaaccttta
    24901 caaaagaagg cgaggcggtt ttaattaaca cgcctgtcta cccacccttt gctcgcagtg
    24961 tcaagttgaa taatcgtaga ttgattacta attccttagt ggaaaaggat ggtctgtttg
    25021 agattgactt tgaccaactt gaaaaggatt tggtggaaga ggaggttaaa ctctatattc
    25081 tttgcaaccc tcacaatcct ggtggacgtg tttgggaaaa agaagtgttg gagaagattg
    25141 gccaactttg ccaaaaacac ggtgttttgt tagtttcgga tgagattcac caagatttga
    25201 ccctctttgg tcacaaacac cagtctttca ataccatcaa tcctgccttc aaaaattttg
    25261 ctatcgtctt gagcagtgcc actaaaacat ttaatattgc tggaacaaaa aattcctatg
    25321 cagtcattga aaatcctaag ttgagactgg ctttccagaa acgcctgttg gccaataatc
    25381 agcatgaaat ttcaggcttg ggttatttgg cgacagaagc tgcctatcga tacggtaaag
    25441 attggctaga ggaactcaag caagtctttg aagaccacat caattatgtg gtggatctat
    25501 ttggaaaaga gactaaaatc aaggtcatga aaccgcaagg tacctacttg atttggcttg
    25561 acttttcagc ctatgacctg actgatgaaa cattgcaaga gttgttgaga aatgaagcca
    25621 aggttatcct caaccgtggt ttggattttg gagaggaagg aagtctccat gcccgcatca
    25681 atatagctat gcccaaatct ctgttgcaag aagtctgtca gcggattgtg gctacttttg
    25741 ccaaacgtta aaaatccagc cttctaggag aaaagtcttc ctagaaggct attttcatag
    25801 gcgaaaatat ggtataataa aaagataagg taaaggtgaa aatatggcta aattgattcc
    25861 ggggaaaatt cgtatcgaag gtgttgccct ttatgaaact ggtaaggttg atatcatcaa
    25921 ggaaaagaac aatcggctct acgctcgcgt tgcagaagaa gaactgcgct atagtttaga
    25981 ggatgatttg gttttttgtg cctgtgattc ttttcaaaag aggggctact gtgtgcattt
    26041 ggcagcgcta gagcattttc tgaaaaatga tgagcgtggt caggaaatct tgtggagtct
    26101 ggaagaaggt catgaagaaa aagaggccgt tgaaaccaag gtgaccttgg gtggcaagtt
    26161 tttgaatcga attttatctc caaaatcaga atgcgcctat gagttatcag ctgtgggtca
    26221 ggtggaagca ggaaccaatc atatcttgtg gactctgaga atcggccaaa tcaatagcca
    26281 aaaatactat gtcattcggg atattccact ttttttaagg attgtcgagc agagaaagtc
    26341 gtatatgatt ggcaagattt atgaggagtc tctttcttgg gaagcctttg atgatgccag
    26401 ccaagaactt ttgactttct tgcacggact aacggaggaa gggctggttc cagacctctt
    26461 tttccaaaac caaggtcggc acctcttttt ccctctgacc ttttttgagc agggtgtgaa
    26521 tttactgatg gccttgcccc attttcaatt tgaccatcaa gtggatagct accaaaccct
    26581 gctttttcag gatatgcatg ctgatgccaa tctatttgcc tttacagtaa cagaatactc
    26641 ggactatttt gaaatggaaa tcagtgagag tcctagggtc aatgtctttt atcagggagc
    26701 agtacttttc cataaaggtc aggtttattt tttaacagac cagcagatgc gtcttctcaa
    26761 ggaaattaaa gcgttacctg tgggtcaaca aggaaagaaa tacctgcaat ttgatagcag
    26821 tgatcgagac aaattggcct cctgtctgac tctttttatc cagatgggca ccgtttcagc
    26881 tccagaacgt ctacaaatca aaacttttgc gccctctttt tactttgaca gggaagaaga
    26941 taatcggatt cgtttagaaa ttcagtttga ttatggaaat agacaggtat ctagccgaca
    27001 agagctagaa gaattaccat tttcaagtga cgcggactta gaagaaagaa ttttccaagt
    27061 ctgtttggct gcgggctttg aagccgattt tcaatcttgg cgtcaggcct taaaagcgga
    27121 gtctgtctat catttttttc atgatatcat tccagtcttt gaaaaactcg ggcatgttga
    27181 cctatcagat aaactagaag agctttatag cctagcgagt cctcaggtgc agattgcctc
    27241 caagggaggt ctcttggaaa tccagtttga ttttcaaaat attgcccagg aggaaatcga
    27301 ccaagccatg caggccttgg ttgccaatca ggatttttat attgattcgt ctaatcaagt
    27361 ttactttttc gatgaagaaa ccaagaaaat tcgccaaaat ctacaggaac tggggcaatt
    27421 tgaattaaaa gatgggacct tgcaggctcg gaaatccttg gcctacagtt tagctcatct
    27481 ctttgaaggg cgagaccgtg tttctttttc acaagaattc cagaatctgg cccaggattt
    27541 gacgcatcca gaggactttc ctttgcaagc aactcaggtc aaggctgact tgcgagatta
    27601 tcaggaaaag ggaattggct ggttgcagat gctccatcat tatggttttg gtgggatttt
    27661 ggctgatgat atgggacttg ggaaaaccct tcagaccatt gcttttttga ctagtcaagt
    27721 gacaaaagaa agtcgagtct tgattctcgc tccgtcgggt ttgatttaca actgggcgga
    27781 tgagtttcag aaatttgccc cacagttgga tgtggctgtt gttcatggtt tgaaagcaag
    27841 tcgtgaggag attcttgccg agagccatca aatctatgtg actagctatg ccaccttccg
    27901 tcaggacagt gagttttatc aggggatggc ctttgacttc cttttcttag atgaggctca
    27961 ggtcatgaaa aatgcccaga ccaagattgc ccagaccttg agacaatttg tggtgccgtc
    28021 ggtctttgcc ttgtcaggaa ctccgattga aaaccatctg ggtgagttgt ggtctatttt
    28081 ccaaatcgtc atgcccggac ttttgccaag caagaaagaa tttatgaaat tgccagcaga
    28141 gcgagtggct cagtttatca agcctttcgt gatgcgacgc aagaaagaag aagttctgac
    28201 tgaattgcca gacttgattg aggtggttta taaaaatgaa ctggaagacc agcaaaaggc
    28261 tatttacctt gcccagttac aacagatgcg agaccatctg gctcaagtgt cagaacagga
    28321 atttcagcga agtcgtgtgg aaattttatc tggtttgatg cgcttgcgtc aaatatgtga
    28381 cactcctgcc ctgtttatgg aagattatca gggagccagc ggcaaactag atagtctccg
    28441 agacctactg gtacaggtgg cagacggcgg acaccgtgtc ttgattttct ctcagttcaa
    28501 gggaatgttg gaaaaaattg aacaagaact gccagacttg ggcctgactt cctttaaaat
    28561 tacgggttca accccagcca aggaaagaca agacatgacc aaggccttta atcaaggaga
    28621 aagagatgcc tttctgattt ccctcaaggc tggtggggtc ggtctgaacc tgacaggtgc
    28681 agatacagtg atcttggttg acctttggtg gaatcctgcg gtggaagcgc aagccattgg
    28741 ccgtgcccat cggatgggtc aggaagaaac ggttgaggtc tatcgcttgg tgaccaaggg
    28801 aaccattgaa gaaaaaattc aggaactcca agaacaaaag aaacatctgg tgtcacaagt
    28861 attggacggc acagagtcac gtggtagtct gaccctagca gaaattagag aaattttggg
    28921 aatttctgaa gccagcactt gaaaaatcaa gacaatacgc tataatgaag tgttgaaagg
    28981 aaatagaaaa tgaaacaaga acgatttcca ttggtgtcag atgacgaggt catgttgact
    29041 gaaatgccag tcatgaatct ctatgatgag tctgatctga tcagtaatat caagggtgag
    29101 tatcgagata aaaattattt agaatgggct cctattgctg aagaaaaacc agtaaaaccg
    29161 attgaaaagc aagtagggaa acctaaaaaa gctcctttag gggttaaaaa agaaggaaag
    29221 agctatgcgg aggtggcgcg tgaagaagcg cgtgcagact tgaaaaagaa acgctctgct
    29281 aactatctaa ctcaggattt cagccttgcg agacgtcatt ctcagcccag tctagttaga
    29341 cagggcaatc aaccgacaac tcctttccaa aaggaaaatc ctggtgaatt tgtcaaatat
    29401 agccaaaaat tgacccagtc tcattatatc ttggcggaag aagttcattc tatccctacc
    29461 aagaatgaag aagtgtcagc acctgctcca aagaaaaaca attatgattt tctaaagaag
    29521 agccaaatct acaataaaaa aagtaaacaa acagaacaag aacgtcgggt tgcccaagag
    29581 ttgaatctga ccagaatgac agaataggga gaaaacatgt caaagacata tcattttatc
    29641 ggaattaagg gatcagggat gagtgccttg gccttgatgt tgcaccagat ggggcacaag
    29701 gttcagggat cagatgttga aaagtactac tttacccaac gcggtcttga gcaggcagga
    29761 attaccattc ttccttttga tgaaaagaat ctagacggtg atatggaaat tatcgctgga
    29821 aatgcctttc gtccagataa caacgtcgaa attgcctatg cggaccaaaa tggtatcagc
    29881 tacaaacgtt accatgagtt tctaggtagc tttatgcgtg actttgttag catgggagta
    29941 gcaggagcac atggaaaaac ttcaacgaca ggtatgttgt ctcatgtttt gtctcacatt
    30001 acagatacca gcttcttgat tggagatggg acaggtcgtg gttcggccaa tgccaaatat
    30061 tttgtctttg aatctgacga atatgagcgt cacttcatgc cttaccaccc agaatactct
    30121 attatcacca acattgactt tgaccatcca gattatttca caagtctcga ggatgttttc
    30181 aatgccttta acgactatgc caaacaaatc accaagggtc tttttgtcta tggtgaagat
    30241 gctgaattgc gtaagattac gtctgatgca ccaatttatt attatggttt tgaagctgaa
    30301 ggcaatgact ttgtagctag tgatcttctt cgttcaacaa ctggttcaac cttcaccgtt
    30361 catttccgtg gacaaaactt ggggcaattc cacattccaa cctttggtcg tcacaatatc
    30421 atgaatgcga cagccgttat tggtcttctt tacacagcag gatttgattt gaacttggtg
    30481 cgtgagcact tgaaaacatt ttccggtgtt aaacgtcgtt tcactgagaa aattgtcaat
    30541 gatacagtga ttatcgatga ctttgcccat catccaacag aaattattgc gaccttggat
    30601 gcggctcgtc agaaataccc aagcaaggaa attgtagcag tctttcaacc gcataccttt
    30661 acaagaacca ttgccttgtt ggacgacttt gcccatgctt taaaccaagc agatgctgtt
    30721 tatctagcgc aaatttatgg ctcggctcgt gaagtagatc atggtgacgt taaggtagaa
    30781 gacctagcca acaaaatcaa caaaaaacac caagtgatta ctgttgaaaa tgtttctccg
    30841 ctcctagacc atgacaatgc tgtttacgtc tttatgggag caggagacat ccaaacctat
    30901 gaatactcat ttgagcgtct cttgtctaac ttgacaagca atgttcaata ggatgttccc
    30961 atggaaattc caattaagat cattcaggca agcaagtctg atttgcctga gataggggca
    31021 cttcagacct cgtcttttcc agctgaaaag cagcaacttt cccatatttt agaagaaagt
    31081 atccgtaagt gtgcggatac ctttcttcta gctagggatg aaaatcaact tttaggctat
    31141 attttatcaa gtccccagtc agacaatccg caatatctaa aagtacattc tttagtcatc
    31201 gagtctgacc atcagagaca gggcttggga acacttcttc ttgcagcctt gaaagaggtg
    31261 gcagttgatc tggattacaa agggattcgt ttggagagtc ctgatgagct gctttcctat
    31321 tttgaaatga acggttttgt tgatgaagaa gcaactttgc tctatgcaac tagccagggc
    31381 tatagtatga tttggtttaa tcccttttat ctggaggaac aatgaaaatc agacaagcaa
    31441 gattagaaga tttggatcgg attgttgaac ttgaatttga aaatttttcg gtcgaagaag
    31501 ccattcctcc ttctgtcttt gaagcacatt tgagagaaat tcagacctct tttctggttg
    31561 ctgaaaaaga aggaagaatc atgggttata tcgaaggacc agttggcctg caccgccatc
    31621 tgcaagacca gtcttttaca gaagaaataa aagactatag tcatgagcct ggtggttata
    31681 tctctgtgac ctgtctgtct attgccaagg aggcacaggg actcggactg ggtcagaaat
    31741 tgctgacagc tttgaaagaa gttgctcttg aagatgagag agatggcatt aatctaacct
    31801 gtcatgacta tctcatcgcc tattatgaaa aacatggatt tgtcaacgaa ggccagtccc
    31861 agtcaacctt tgcaggggaa acatggtatg atatggtctg ggaaatgaaa aaataagtta
    31921 ggaaaagtat catagattgc tttttttgga cttttaagat ataatattat ggattatcaa
    31981 caaggaggaa aaacttttga gtgaaaagtc aagagaagaa gagaaattaa gctttaaaga
    32041 gcagattctg agagatttag aaaaagtaaa aggctatgat gaagttctga aagaagatga
    32101 ggcagtagtt cgcactcctg caaatgaacc ttcagctgaa gaactcatga ctgattcctt
    32161 gtcaacggta gaggagatta tgagaaaagc tcctaccgtg cctactcacc caagtcaagg
    32221 tgtaccagct tctccagcag atgagattca aagagaaact cctggtgttc caagtcatcc
    32281 aagtcaagat gtaccttctt ctccagcgga agaaagtgga tcaagaccag gtcctgttag
    32341 acctaagaaa cttgaaagag aatacaatga aaccccaaca agggtagctg tttcctatac
    32401 gacggcagag aaaaaagcag aacaagcagg tccagaaaca cctacgcctg ctacagaaac
    32461 agtggatatc atcagcgata catcacgtcg tagccgtaga gaaggagcaa aacccgttaa
    32521 tcctaagaaa gagaagaagt cacatgtgaa agcttttgtg atttcattcc ttgtattcct
    32581 tgccttgctc tcagcaggtg gttactttgg ttaccagtac gtgctagatt ctttgttacc
    32641 tatcgatgct aattctaaga aatatgtgac ggttggaatt ccagaaggtt caaacgttca
    32701 agaaatcggt acgacgcttg aaaaagctgg tttggtaaag catggtctga tttttagttt
    32761 ttatgccaag tataaaaatt ataccgactt gaaagcaggt tactacaatt tgcaaaagag
    32821 tatgagtaca gaagacttac tcaaagagtt gcaaaaaggt ggaacagatg aaccgcaaga
    32881 acctgtactt gcgactttga caattccaga aggttatacc ttggatcaga ttgctcaagc
    32941 tgtgggtcaa ttgcaaggtg acttcaaaga gtctttgaca gcggaggctt tcttggctaa
    33001 agttcaagat gagacgttta tcagtcaagc agtagcgaaa tatcctactt tactggaaag
    33061 tttgcctgta aaagacagcg gtgcgcgtta tcgtttggaa ggataccttt tcccagctac
    33121 atactctatc aaggaaagca caactattga gagcttgatt gatgagatgt tagctgctat
    33181 ggataagaac ctatctcctt actatagtac tatcaaatct aaaaatttga ctgtcaatga
    33241 gttgttgacc attgcttcct tggtcgaaaa agaaggtgcc aagacagaag atcgtaagct
    33301 cattgcaggt gtattctaca atcgtttaaa tcgtgatatg ccacttcaaa gtaatattgc
    33361 aatcttgtat gcccaaggaa aactggggca aaatatcagt ctagctgagg atgttgcgat
    33421 tgataccaac attgattcac cttataatgt ttataaaaat gtaggtctca tgcctggtcc
    33481 agtcgatagt ccaagtctgg atgcgattga gtcaagcatc aatcaaacta agagcgataa
    33541 cctctacttt gtagcagatg tcacagaagg caaggtctac tatgctaaca atcaagaaga
    33601 ccacgaccgc aatgtcgctg aacatgtcaa cagcaaatta aactaaacaa actaaaatta
    33661 tgtgatacgt cacataattt tctttagaaa atattatcag aagaaagttg agaaaaatgg
    33721 cagaaaaaac atatcctatg acccttgagg aaaaggaaaa acttgaaaaa gaattagaag
    33781 aattgaaatt ggttcgtcga ccagaagtgg tagaacgcat taagattgcc cgttcatacg
    33841 gtgacctttc agaaaacagt gagtacgaag cagctaagga tgaacaagcc tttgtcgaag
    33901 gacaaatctc tagcttagaa acaaaaatcc gctatgctga aatcgtcaat agcgacgcag
    33961 ttgcccagga cgaagtagcg attggtaaaa cagtcaccat ccaagaaatt ggtgaggacg
    34021 aagaagaagt ttatattatc gtaggttcag ctggtgcgga tgcctttgca ggtaaggttt
    34081 caaatgaaag cccaattggg caggccttga ttggcaagaa aacaggtgac acagcaacca
    34141 ttgaaacgcc tgttggtagc tatgatgtaa aaatcttgaa ggttgaaaaa acagcctaaa
    34201 aacagaaaaa ggagtgggga ggcgatgtgc ttcactcact cctttttcca ttttgctatt
    34261 cttcgaaaat ctcttcaaac cacgtcagcg tcgccttgcc gtatgtatgg ttactgactt
    34321 tgtcagtttc atctacaacc tcaaaac
//
LOCUS       DAVBCN010000013        34203 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVBCN010000013 DAVBCN010000000
VERSION     DAVBCN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34203
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.013"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(181..1167)
                     /locus_tag="VQ497_000599"
     CDS             complement(181..1167)
                     /locus_tag="VQ497_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2516948.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(1177..1977)
                     /locus_tag="VQ497_000600"
     CDS             complement(1177..1977)
                     /locus_tag="VQ497_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HET2516949.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRIYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(2230..3072)
                     /locus_tag="VQ497_000601"
     CDS             complement(2230..3072)
                     /locus_tag="VQ497_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2516950.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(3074..4366)
                     /locus_tag="VQ497_000602"
     CDS             complement(3074..4366)
                     /locus_tag="VQ497_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2516951.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(4477..5748)
                     /locus_tag="VQ497_000603"
     CDS             complement(4477..5748)
                     /locus_tag="VQ497_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2516952.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            6298..7815
                     /gene="malQ"
                     /locus_tag="VQ497_000604"
     CDS             6298..7815
                     /gene="malQ"
                     /locus_tag="VQ497_000604"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET2516953.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLGASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEHFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            7841..10099
                     /gene="glgP"
                     /locus_tag="VQ497_000605"
     CDS             7841..10099
                     /gene="glgP"
                     /locus_tag="VQ497_000605"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HET2516954.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLKSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(11144..11716)
                     /locus_tag="VQ497_000606"
     CDS             complement(11144..11716)
                     /locus_tag="VQ497_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516955.1"
                     /translation="MDKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHVAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(12338..13186)
                     /locus_tag="VQ497_000607"
     CDS             complement(12338..13186)
                     /locus_tag="VQ497_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET2516956.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYNKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            13338..13616
                     /locus_tag="VQ497_000608"
     CDS             13338..13616
                     /locus_tag="VQ497_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516957.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            13618..15681
                     /locus_tag="VQ497_000609"
     CDS             13618..15681
                     /locus_tag="VQ497_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET2516958.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIEFIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIKLMKKSEENKKTKQRYFIKMADKVVKYNFFGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(15759..17015)
                     /locus_tag="VQ497_000610"
     CDS             complement(15759..17015)
                     /locus_tag="VQ497_000610"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET2516959.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKVFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            17157..19622
                     /locus_tag="VQ497_000611"
     CDS             17157..19622
                     /locus_tag="VQ497_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET2516960.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVAGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNLTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGALATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            19687..20598
                     /locus_tag="VQ497_000612"
     CDS             19687..20598
                     /locus_tag="VQ497_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET2516961.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            20828..21526
                     /gene="dapD"
                     /locus_tag="VQ497_000613"
     CDS             20828..21526
                     /gene="dapD"
                     /locus_tag="VQ497_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HET2516962.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVCVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            21594..22724
                     /locus_tag="VQ497_000614"
     CDS             21594..22724
                     /locus_tag="VQ497_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HET2516963.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFTSQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            22736..23275
                     /locus_tag="VQ497_000615"
     CDS             22736..23275
                     /locus_tag="VQ497_000615"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET2516964.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLTPKTYPKGRMDFVVYDPQQLVKTSFALLEPQ
                     GDLEVVDASQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGRALSTIYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            23259..23936
                     /locus_tag="VQ497_000616"
     CDS             23259..23936
                     /locus_tag="VQ497_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET2516965.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(24008..24907)
                     /gene="galU"
                     /locus_tag="VQ497_000617"
     CDS             complement(24008..24907)
                     /gene="galU"
                     /locus_tag="VQ497_000617"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HET2516966.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(24929..25945)
                     /locus_tag="VQ497_000618"
     CDS             complement(24929..25945)
                     /locus_tag="VQ497_000618"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET2516967.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     YHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKAI
                     IMHASKGLEPDSHKRLSTILEEEIPEQLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            26157..26536
                     /locus_tag="VQ497_000619"
                     /pseudo
     CDS             26157..26536
                     /locus_tag="VQ497_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(26716..27141)
                     /locus_tag="VQ497_000620"
     CDS             complement(26716..27141)
                     /locus_tag="VQ497_000620"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2516968.1"
                     /translation="MNNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKSLTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(27209..27859)
                     /gene="phoU"
                     /locus_tag="VQ497_000621"
     CDS             complement(27209..27859)
                     /gene="phoU"
                     /locus_tag="VQ497_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET2516969.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(27874..28626)
                     /gene="pstB"
                     /locus_tag="VQ497_000622"
     CDS             complement(27874..28626)
                     /gene="pstB"
                     /locus_tag="VQ497_000622"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2516970.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDVLLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(28628..29443)
                     /gene="pstA"
                     /locus_tag="VQ497_000623"
     CDS             complement(28628..29443)
                     /gene="pstA"
                     /locus_tag="VQ497_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET2516971.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(29436..30299)
                     /gene="pstC"
                     /locus_tag="VQ497_000624"
     CDS             complement(29436..30299)
                     /gene="pstC"
                     /locus_tag="VQ497_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET2516972.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRTFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(30417..31292)
                     /locus_tag="VQ497_000625"
     CDS             complement(30417..31292)
                     /locus_tag="VQ497_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HET2516973.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(31459..32790)
                     /locus_tag="VQ497_000626"
     CDS             complement(31459..32790)
                     /locus_tag="VQ497_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HET2516974.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSALKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(32791..33498)
                     /locus_tag="VQ497_000627"
     CDS             complement(32791..33498)
                     /locus_tag="VQ497_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2516975.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(33582..34067)
                     /locus_tag="VQ497_000628"
     CDS             complement(33582..34067)
                     /locus_tag="VQ497_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET2516976.1"
                     /translation="MAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDYALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            34053..34203
                     /locus_tag="VQ497_000629"
                     /pseudo
     CDS             34053..34203
                     /locus_tag="VQ497_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT        10070 a         6448 c         7337 g        10348 t
ORIGIN      
        1 acctgcggct agcttcctag tttgcacttt gattttcatt gagtataaaa agatcttggg
       61 aaaagactca atttcagtag aaaaggaagt aaatcttccc acattaaaac gcatagtatc
      121 aagttttttc aatacctgat agtatgcgtt tttgctttta acgacttttt gcccagtctc
      181 ttatttttcg ataattttgt gggcgatcaa ttgacggtaa caaatttgtt tattgttttt
      241 attatcatta ataatctgga gaatagtttc aagggaaaca cgaccaagct ctaaactatt
      301 gatatcgaca taggctgcca agttgagctt gggattaacc gagtcaaagc tgagaacagg
      361 gacatccagc tggtgtttgg caatatagtt acaaacgcct tcagctagga ggctatcggt
      421 tgtgatgata gcatcaattt gtggatcgtg cttgaataat cgcttgctaa atttatagcc
      481 cttttcttcc agaaactcgt cggcaaagta gatgcgattg ttgtcagtgg taagtttgta
      541 atgtttaagc gcctgttcat agcctgttaa acggtctttg gtcacgaaga gctttttact
      601 tcctccgata aaggcaatgc gtttgcagcc ttttttgatg aaatattcag tcgcatcaaa
      661 accagcttga acattgtcgt tgtcgacaag tgggatgaaa ggagatagag atttacctaa
      721 gataaggaag gggaactgtt cttctgcgac gagttttacg agagggtctt cttcttgggc
      781 atagagaaag attagcccat ctacacgctt gccgtagacc atttgtgaaa tagcgttgag
      841 acgctccttc tcatctttcc ctgttgctat ctgaatggca tagtggtttt cagatgcgac
      901 ttgagagatg ccacgtagaa ccgatggaaa gaaaggattc tggtagaagg cgtctgagtc
      961 atcaggaaga actaatccga taacctgagt atagctgctt accaagctac gagcgttgag
     1021 gtttgggtgg tagttgagtt ccttcatagc tttgcgaacg cgtttttttg tttcgtcgct
     1081 aatggttgat ttattttgaa taacacgggt tacggttgaa ggcgaaacac cagcagcctt
     1141 ggccacgtct ttaatcgtaa cgggcataaa aatctcctat ttatggtaat ttggatcacg
     1201 gaaatgtgtt ttataaaaga tagtgaccag atacagaaca aaaagaatat tttgtacagt
     1261 aatcagggtt gtcatatttt ggccaaataa tcccaaaata agtgtaatca gagtcggcaa
     1321 tcctaaacag ttcaagataa agtgatagca ctctttaaag gttctaaatg aaaagaggcg
     1381 tgatttctta gtgatataaa ggagaagact agctcctaga gagacgataa agaaattcaa
     1441 accaaagagg aagctcgcac cgagaactag gaagaggctg atatagatac gattttgttg
     1501 gtaccagtct ttagaaattg cttgggtcaa gctgtctttg cttttgaaac tctcagtctg
     1561 aatggctcgg taagagatgc gggtcagttc cttgctttcc ttgctgatga ctagttcatt
     1621 tgtatcgaaa tgcagttgca agtccttagg taattccttg atttgacttg gaccaatcac
     1681 aacagaaggg gcttgactag ctgttccagt ataagttaat gtgccatcga caattgtagc
     1741 atgttcagag agatcctgga caacctcatt tgtcagaggt tcatagacat tatcgataaa
     1801 agtttctagc ggataggtct cctgggagct gttttggata gcaatgggta ccatagacaa
     1861 gctgataagg aagatactgg taaagagaag ttgaaaccag ttgagctcga aacgtttgga
     1921 caggggctta cgaaaccccc aaatacttga aaaataggaa aatggatatg gaagcatagg
     1981 tatctttcta aaaggtgttt tctatatgag tattattata tagagaaatg cgctttattt
     2041 caagtatagg gggtaaattg cttgctacaa ggatattatt tacagtgaca attgctaata
     2101 atcaatgaaa atcaaagtgc aaactagcca caggttgctc aaagcacaac tttgaggttg
     2161 gagataaagc tgacgtggtt tgaagagatt ttcgaagagt ataaaatgaa aaagggtggc
     2221 ggggataaat tatcccttgt cgccaccact tgtaagtcct gaaacaaagt tcttttgtag
     2281 gaagaagaag agaatacaga ttggaagggc gatgaggata gcacctgctg agaagtaggc
     2341 aatcttcaag tttttcgcat tgttaacgaa ggtttggaga cctacggcaa cagtaaagta
     2401 ttctttctca cgaagcaaga aactagagag gatgtagtcc ccgaaaggtc ccatgaaggc
     2461 ccagagagct tgtacggcaa ccattgggcg aacaagtggt agaacaattt gccagaagcg
     2521 gcggaagtgt cctgcaccgt ctagttttgc agattcgtct aaagacattg gcactgtatc
     2581 gaagtagcct ttcatgagcc aagcattcat cgggatacca ccaccaacgt agaggaagat
     2641 gaggaaccag ttgtggttaa gggcgttcaa cataagcgcc ataacgaaga aggctgtcaa
     2701 agcggccata gttggcacca tttggatgat caagaagaag accaaacttt gtttacgagc
     2761 caagaagttg taacggctgt aggcataacc agcaagtacg atgatacttg tttgaacagc
     2821 catggtaatt aaggcgataa tcaaagtgtt gaggtaccaa gtaccgtaca aggtttcagt
     2881 gaagaggcct ttaaagttat caaaattgag gtcgatatta gtatctagtt taaaggctga
     2941 gacgttacct gctttaaagg ctgacataat ggtaatcaac agtggataga taattacaat
     3001 tgatagacca atcaggtaaa ggtaagtaag gctttgagtc agtctacgtt tgagtttaat
     3061 tgagttattc atcttagacg tcctccatat caaatgcgtg tagtttcttg aatgcgatca
     3121 tagagattga gatgacaatg atagagataa tcaaggtaac agctgccgcc attgagtatt
     3181 gaggagatgt acctgttgtc aaacggtaga tccatgagat caagatatcg gttgaaccag
     3241 ctccacctcc gacactacca ggtcctccac cattgaagag gtacatgata gagaagttgt
     3301 taaagttgaa ggtgtattgg ctaatcaaag taggtgccgc aacagccaaa atcattggga
     3361 aagtgatgtt gcggaatttt tgccaagcgt tggcaccgtc aatataagct gcttcgtaaa
     3421 ggtcgttagg aatagattgc aagataccca aagtcaaaac gtagatgtat gggaatccga
     3481 gccaaccttg catcataatc aaggcaatct tagtccaagt tgggtctgtt ttccaaggaa
     3541 taagagctcc atcaaggaaa ggaaggaatt tagccaagat tggcaatact tgagtgttga
     3601 tagcaccgac actatcgtta aacatgtttg agaatgtcaa gatagtgatg aaggctggga
     3661 cagcccaagg aagaaggaaa ataacaccaa agatacgttt tcctttgata aatggttggt
     3721 tggcaatgat agctgtgaag ataccaatta cgatttgtaa agtagaagct gccaaagccc
     3781 aaatgatagt ccaagaaaga acagaaccaa aggcagaacg gaaggtactc aagctccaaa
     3841 tgtttgtaaa gttggtcaaa ccaacccagt ccaacaactt gtttggtggc aagtgttgga
     3901 agtcgtagtt ggtaaaggcg atcatcaagg ttacgataac tgggaagata atcgcgaagg
     3961 tcatggcaac ataagatgga atgatcaaga ggtaagggaa gccattttca taaatccctt
     4021 tgatcatgtc tttgagtgtg cgtggaactg gaattccatt gttaatgcgt tttgcaatcg
     4081 tatgtgcatc tttgatattt gagaaataaa agagtacata aacgattaca aagattagat
     4141 ggaaggcacc acgaatcagc ataaagaggg aattatcacg acctggtttg tcaccaagag
     4201 tgatgaggtt gctcaattca ggggttgcaa gtgctaggaa gtaaaggaca aatacgatgg
     4261 ttacaccaag gaagataaaa cctttggctt tttgtttatt gtaaatctgt cctaacccag
     4321 gaatgataga cagcagggct gctttactag gttgttgctt ttccatatta actcctttca
     4381 taggatacta cttttttata agaaagctaa attatttgtg tctctattga taaattcaaa
     4441 gggggatttg atttccaccc cccttgaaca aattttttat tcaccaaatt tttgtttgat
     4501 tgtttctttg atcaatgtta cagcatcgtt agcagctgtt ttagcatctt tttgaccact
     4561 tacagcatca aagagcatat ttttcgctgg atcccaaact gcagacattt gagagatgtt
     4621 tggcagtggt tgagtgttct tgaactgttt gataacagct gttgtcaact catcgttttt
     4681 accttcagcg tatgaacgag cctcagtatt agctgggatt tcgttagtct tatcatataa
     4741 tactttttgt tgttcagttg caacaaggaa gtctacaaat ttttgagaag cttcaaggtt
     4801 cttaacggct tgaggaatga cccaagcttt accaccaccg aatgcagcat attcttttcc
     4861 atttggaaga gttgggatag ttgcaactcc gtagtttact ttagcatctt taaaggcttg
     4921 agctttccaa ggtccgtcga tgatagcagc tgttttacct tcttggaatt gagtttggat
     4981 taagtttcca gcaccttctg tatcttgcat acctttaggc catttttcgt accaagattt
     5041 agcgtagttg atacctgcga tagaaccgtc gtttgcaaga ccgatgtctt tagcgtcttt
     5101 accgttttgg ccaaagacgt aagcaccgtt accggcaaga agtccatatg tatagtagaa
     5161 gtttgtccag tcagctagga aggcagtagt tttaccatct tcaccagcga atgcgtattt
     5221 gctatcttta gcaaggtttt ccaagtcagc aaatgttttt ggagcatctt tcaccaagtc
     5281 tttgttgtag tacataacaa gtgactcgat aacggcagga gcaccgtaaa ctttaccatt
     5341 agcagctgtt acaagagatt tagttgtgtc gtctgtttta gcaccatcgc tcaatttcac
     5401 ttctgaaagt tgtccgtcag aaccaaggct acctacacgg tcgtatggag ccatcataac
     5461 atcagggaca ttaccagatt ggttgtcaag agaaagttta tcaagacctc ctagagcatc
     5521 accagtttta agagtgactt ttactccagc ttctttttca taagctttag caacctcttc
     5581 aatatagctc ttatatccct cgtctacata tacagtgagt tctttgactt cagatgaacc
     5641 agaatcagca ggcttatcag cagttttgct tccgcaagct accaaaagca agctagcaag
     5701 tgtagcagtt ccaagcaccg cagtgctctt cataaattta gatgacatag tgtattcctc
     5761 ccaaagaata gcaagtttta ttgataagga aacgcaaacg ttttccttta tgagcttagt
     5821 atagcacaaa tagaaaacgg ttgcaagtat tttttgtaaa aattttaaaa aatttttaag
     5881 cttgatttga tagcataatt ttgcatttta atagaagaaa agtatgaaat agataagaat
     5941 acagtatttt taataaaaat atatggaatc tgtctaaaga gggaaatcag gaggggctac
     6001 ctccctgttt ctaatcccta tctattttct atagacgttt gtgctttgtt gcatataaat
     6061 tcaagctccc taaggaggtt ttgtactttg tagactgtct cgtgagagtc aaactttagg
     6121 attagatagt tttaagttat ctttgctaaa atgagttagt ctccttaaaa ataatcaaaa
     6181 aagtttttta aaaacgcttg caattatgct tgaaaaggag tatacttata agtaacgcaa
     6241 acgtttgcgt ctgcaaaata cgcaacgttc cattatttta acacacgagg tgctattatg
     6301 aaaaaacgtc aaagtggtgt gttgatgcac atctcttctc ttccaggagc ttacggaatc
     6361 ggatcatttg gtcaaagtgc ttacgacttc gttgatttct tggtccgtac aaaacaacgt
     6421 tactggcaaa tccttccatt aggagcaact agttacgggg attctcctta ccaatctttc
     6481 tcagccttcg caggaaacac tcattttatc gatttagata tcttggtgga gcaaggtttg
     6541 ttgggagcga gtgaccttga aggagttgac tttggtagcg atgcgtctga agttgactat
     6601 gctaaaatct actatgcacg tcgtcctctt ttagaaaaag cggtgaaacg tttctttgaa
     6661 gtcggagatg ttaaagattt tgagaaattt gctcaagaca accaatcatg gcttgagctc
     6721 tttgctgagt atatggctat caaagagcat tttgacaatc ttgcttggac tgaatggcca
     6781 gatgcagatg ctcgtgctcg taaagcttca gcacttgaaa gctatcgtga gcaattggca
     6841 gacaagttgg tttaccaccg tgtgactcaa tacttcttct tccaacaatg gttgaaattg
     6901 aaagcttacg ctaacgacaa ccacattgaa atcgttgggg acatgccaat ctacgtagcg
     6961 gaagattcaa gtgatatgtg ggcaaatcca catctcttca aaacagatgt caatggtaag
     7021 gctacttgta tcgcaggatg cccaccagat gagttttctg taactggtca gctttggggt
     7081 aacccaatct atgactggga agcaatggac aaagacggct acaaatggtg gattgaacgc
     7141 ttgcgtgaaa gcttcaaaat ctacgatatc gttcgtatcg accacttccg tggcttcgaa
     7201 tcttattggg aaatccctgc tggttccgat acagcagcac ctggtgagtg ggtgaaaggt
     7261 ccaggctaca agctttttgc agccgttaag gaagaacttg gtgagctaaa catcatcgca
     7321 gaagaccttg gcttcatgac agatgaagtg atcgaattac gtgaacgtac tggcttccca
     7381 ggaatgaaga ttcttcaatt tgccttcaac ccagaagacg aaagcattga tagcccacac
     7441 ttggcacctg ctaactcagt tatgtacaca ggaacacacg ataacaatac ggttcttggt
     7501 tggtaccgta atgagattga tgatgcgact cgtgagtaca tggctcgtta cacgaaccgt
     7561 aaagaatacg aaacagtggt acacgctatg cttcgtacag tattttcatc agttagcttt
     7621 atggcaattg caactatgca agatttacta gaattggatg aggcagctcg tatgaacttc
     7681 ccatctaccc ttggtggaaa ctggtcttgg cgtatgactg aagatcaatt gacaccagct
     7741 gtcgaggaag gtttgcttga cttgacaaca atttatcgcc gaattaatga aaatttggta
     7801 gatttaaaga aataagacaa taatcaggag acaactaaac atgttatcac tacaagaatt
     7861 tgtacaaaat cgttacaata aaaccattgc agaatgtagc aatgaagagc tttaccttgc
     7921 tcttcttaac tacagcaagc ttgcaagcag ccaaaaacca gtcaacactg gtaagaaaaa
     7981 agtttactac atctcagctg agttcttgat tggtaaactc ttgtcaaaca acttgattaa
     8041 ccttggtctt tacgacgatg ttaaaaaaga acttgcagct gcaggtaaag acttgatcga
     8101 agttgaagaa gttgaattgg aaccatctct tggtaatggt ggtttgggac gtttggctgc
     8161 ctgctttatc gactcaattg ctactcttgg tttgaatggt gacggtgttg gtcttaacta
     8221 ccactttggt cttttccaac aagttcttaa aaacaaccaa caagaaacaa ttccaaatgc
     8281 atggttgaca gagcaaaact ggttggttcg ctcaagccgt agctaccaag taccatttgc
     8341 agactttact ttgaaatcaa ctctttacga tattgatgtt actggttatg aaacagcgac
     8401 taaaaaccgc ttgcgtttgt ttgacttgga ttcagttgat tcttctatta ttaaagatgg
     8461 tatcaacttt gacaagacag atatcgctcg caacttgact ctcttccttt acccagatga
     8521 tagtgaccgt caaggtgaat tgctccgtat cttccaacaa tacttcatgg tttcaaacgg
     8581 tgcgcaattg atcatcgacg aagcaatcga aaaaggaagc aacttgcatg accttgctga
     8641 ctacgcagtt gtccaaatca acgatactca cccatcaatg gtgattcctg aattgattcg
     8701 tcttttgact gcacgtggta tcgagcttga cgaagcaatc tcaattgttc gtagcatgac
     8761 tgcctacact aaccacacaa tccttgctga ggcgcttgaa aaatggcctc ttgaattctt
     8821 gcaagaagtg gttcctcact tggtaccaat catcgaagaa ttggaccgtc gtgtgaaggc
     8881 agagtacaaa gatccagctg ttcaaatcat cgatgagagc ggacgtgttc acatggctca
     8941 catggatatc cactacggat acagtgttaa cggggttgca gcacttcata ctgaaatctt
     9001 gaaaaattct gagttgaaag ccttctacga cctttaccca gaaaagttca acaacaaaac
     9061 aaacggtatc actttccgtc gttggcttat gcatgctaac ccaagattgt ctcactactt
     9121 ggatgagatt cttggagatg gttggcacca tgaagcagat gagcttgaaa aactgttgtc
     9181 ttatgaagac aaagcagctg tcaaagaaaa attggaaagc atcaaggctc acaacaaacg
     9241 taaattggct cgtcacttga aagaacacca aggtgtggaa atcaatccaa attctatctt
     9301 tgatatccaa atcaaacgtc ttcacgagta caaacgccaa caaatgaacg ctttgtacgt
     9361 gatccacaaa taccttgaca tcaaagctgg taacatccct gctcgtccaa tcacaatctt
     9421 ctttggtggt aaagcagctc cagcctacac aatcgctcaa gacattatcc atttaatcct
     9481 ttgcatgtca gaagttattg ctaacgatcc agcagtagct ccacacttgc aagtagttat
     9541 ggttgaaaac tacaacgtta ctgcagcaag tttccttatc ccagcatgtg atatctcaga
     9601 acaaatctca cttgcttcta aagaagcttc aggtactggt aacatgaaat tcatgttgaa
     9661 cggagctttg acacttggta ctatggacgg tgctaacgtg gaaatcgctg agttggttgg
     9721 agaagaaaac atctacatct tcggtgaaga ttcagaaact gttatcgacc tttacgcaaa
     9781 agcagcttac aaatcaagcg aattctacgc tcgtgaagct atcaaaccat tggttgactt
     9841 catcgttagt gatgcagttc ttgcagctgg aaacaaagag cgcttggaac gtctttacaa
     9901 tgaattgatc aacaaagact ggttcatgac tcttcttgat ttggaagact acatcaaagt
     9961 caaagagcaa atgcttgctg actacgaaga ccgtgacgca tggttggata aagtcatcgt
    10021 taacatttct aaagcaggat tcttctcatc tgaccgtaca atcgctcagt ataacgaaga
    10081 catctggcac ttgaactaat actcttcgaa aatctcttca aaccacgtca gctttatctg
    10141 caacctcaaa gcggtgcttt gagcaacctg cggctagctt cctagtttgc tctttgattt
    10201 tcattgagta taagatacaa atttatacta atacatcttg taaaaaagcg agtttcgatt
    10261 gaaattcgct tttttaaatg ctgtggattt gggtcaatct tgtctaaaaa tagggaaatc
    10321 ctagatacag tgaaggcttt aaatgctggt ttttactgtc ttcagcctta tattttttcg
    10381 tagttggtta cctcatatct cttgtattcg cttacataaa gtattataat ataattgtag
    10441 gaaagaaggt gtttttatga tatacacact taaattggtg ttgtttatta cctttcttgt
    10501 aataagcttg ttacctgata agatttttgg aaaaaataaa aaaatttgga aaatagtttt
    10561 tgcaatattg acggcagtgg cagcattgtc atttatgtac taagttattt taagaatgta
    10621 gggaaataaa ccctacattc tttttagttt tttctgtttt ctaaattcta tttatccaag
    10681 cgcttcaaca tttcttgctt cttcgcttca agttctgcac gcttttcttc gatttcggca
    10741 tgttttttct cgagttcaga acaacttgca tcattgctaa attcttttcg ccatcaggag
    10801 atagggtgag tcgacatgtc tattactcac ccaaagcagt cctacaaagc aggaattttc
    10861 tgttactttt ttggaaatag taacgtttat acagctttga cacttcgtat caaagcgcca
    10921 aacacactcc gagggattta cagaaagcag aaaaggaatg atctggtata agatcattcc
    10981 ttttctctct ttttctttaa gtaattatat acaatgtacg acgaagtcgt cattgcaatg
    11041 ctgatccacc acctaaaggg aactttaaac aacattgata agataaagaa tataaacaac
    11101 gaaaatacgt tatacccaat taattttatt gtatatctca tgattaaaag ttaatccttc
    11161 cgttgttagg aatggcatca tttttatccc ataattgtgc taaataagtc cccggtgata
    11221 ataaattcat agcgaattct aaagcaacat catttacaaa ccaactacct agatatctag
    11281 aaattgctga acgaatagca ctttttgcta catgttttcc ttttacttta attagatttg
    11341 caaggcctgc agtagttcct cctaatgcta aagctattgc agtatctaat agagcaccca
    11401 tttgattaac tgtaatacct tgccaagctg ctctaaatgg agagtatgta ggtgggattg
    11461 tataatcgcc ttgtaattgt cggttaatta cttctttgat ccattgttgt gagacgtctg
    11521 gatgaaaaga ttggatttcg tttgcaagtg tattgatttg ttcttctgtt agagaagtga
    11581 caggttgaag ttccatattt gtttcaattt gtgatacttg ttcagaagcg tatacagctg
    11641 aaacacttgg aatcgctgat acaattaaca caattgacgt caaaaaaacc gaaataaatt
    11701 tcattaattt gtccatgagc ttttctcctt tttatttgta tctgcttaca ttttatcata
    11761 tactgttatt atagtcaaaa aaatatgcta ttatgttaaa aaaatatttt tcaaaatata
    11821 aatggacgga tttattttgg attttatttg ttattttgac ctgcctctat attggtaacc
    11881 atgatttgtt tactctcaat catcaagaat tctcttttcg tggtagcgtt tggggtctgg
    11941 tactggcctt atatcactta ttgttcattg ataagtttgt aatttcgaat cgaaaataaa
    12001 gattagaact atgattgact ggctactttt aatattgatt ttggaggaag attttgtctc
    12061 tattatttat tattttaaat ttatttattt tgtataagat ctattctttg aggcgggaaa
    12121 aatcgaaata cttgatttat acggcctata tcatatttgg ggtaaatgta gtatatggta
    12181 ttcaatggtt attaagagaa ctgatttcaa ctatttcccc ttaatgtgta tggaaactat
    12241 aacaaaacgc ataagattaa ggtttgtgta cctgttctta tgcgtttttg tcatattgaa
    12301 gattttcggt aagttttata cctttgggaa gtctagttta gaatgctttc ccaaccaaaa
    12361 tctctgcttc gatggtaatc tctgtcagtt ggctccagtc aatcttgctc tggtcaacgt
    12421 gaaagaggag aggagtcata ctgagtaggg cttggagttg ttctgctgtg atagtcttag
    12481 tcagagaggc agtttgacta gataggatgg taaagtgttc ctggaaatgc tccttgatat
    12541 cttggttaga atactccttg tttgtcagct ggtcctgtac tctttgacgg atttctttga
    12601 ggtgattttc agttgggata acctttatca agataccgtc tttggataaa acgcgacgaa
    12661 attctccata gttggcaggt gagaagatat caagcagaat atccatgtta gcgtctttta
    12721 taggaagtcg tgccaagtcg ccaacaaacc aattgactgc ccagttgggt tcacttttag
    12781 cagcgatttg gactgaatct ttggagatgt caaaggcata gaaagttttt tcagagtgac
    12841 tttcttgtag tttgcgagaa tagaatcctt caccacaacc gatatccaaa attgttgtgg
    12901 tagtttttga gcttgcaagc aagtcagata cagcatctaa gatagcttgg taaaagccgg
    12961 cttctaggat ttgttgacgg ttttgaaaat tttccttgtt atagttagca gattgcttga
    13021 tttgaggtgc tagattgaca tagccaaatt tcgccaagtc aaaagaatga cggttgcagc
    13081 acttgaaatt agtctctaac agagtcagat tttcttgaca gataggacag gcaaaggcag
    13141 tcgcagaagc aaaacgctga agtttgggct tgagatttgt attcatagtt taagtgtatc
    13201 aaaagaggca aatgaaagca actttaggaa taagtagatg ggagattcag tagaaataaa
    13261 actaattctc caatttatag aatgaaattg cttttatatc taaattgcta tataataatg
    13321 ataaggagga aataagtatg ttaaaagaag tattaaccgt tgcaaaagtt gcgaaaaaat
    13381 cttcattatt tttgggtggt gtcgcatttg gtacccttgg tttgaaaatc ttagcaagta
    13441 aggaagctaa aaaaggttat tctaaagctt tggctaaggc ttacaagttg aaagacgagc
    13501 tagatgcatc tgtttctgtt gtgaagcaac atggagacga tgtcttgcaa gatgccaaat
    13561 atttgtacga gcaagagaaa aaagaagagc aattagatag ccttataggt gaataatatg
    13621 tcttttaaag tgctacatag aggataccaa catatccgac tatcatcttc tttttcactt
    13681 accttggata ttcaagacta tcttcgttcc ttggcgagag atgaaaaggg gattgagttt
    13741 atccagtttt acatggatca acagcacttt actctacgca taaaagaagg cttttctgta
    13801 ttagataatg cagaagcctt tttaaaaaga attgataaag ggaaagtttc tgagttgatg
    13861 actcttccca ttcgtagaga agagagtgct tattctattg tttcaggtgc agcggttaag
    13921 cgtgtacttt ttcgtagttt tgtgccgtat cctattcgct atatatggac ttgttatcag
    13981 gctttgggtt atattagaga agcctatcaa acactagcgc gtaaggaact aacgatggaa
    14041 gtcttggact gttcggctat tttattgtct ttgtttatga accaatccaa gacagctagc
    14101 aatatcatgt ttatgcttga tttggggaat catttagatc agtggtcctt gaaaaaaact
    14161 gcaacagatt tagaacagag tcttcttgca aaagagagcg atgtattcct agtacagggc
    14221 gatacggttg ttagtatcaa gagttccgat gttcaaatag gagatgtctt gatcttatct
    14281 caaggaaatg aaattctgtt tgatggacaa gtagtttcag gtttaggtat ggtcaacgaa
    14341 agttccttga caggagagag ttttccagtt gaaaaaagag agtctgattt ggtttgtgca
    14401 aatacagtat tagaaactgg agagttacgc attcgtgtaa cagataatca gatgaacagc
    14461 cgtattttac agctgattaa gttgatgaag aaatctgaag aaaacaagaa aacgaaacaa
    14521 cgctatttca tcaagatggc ggataaagtc gtcaaatata atttctttgg ggctgggctg
    14581 acttacctat tgacaggttc tttttctaaa gctatttctt tcctattggt cgatttctcc
    14641 tgcgctttga aaatctctac tcctgtagct tatttgacag ttatcaagga agggttgaac
    14701 cgtgaaatgg tgattaagga tggagatgtt ctggagaaat atctggaagt tgatactttc
    14761 ttgtttgata agacaggaac aatcacaact agttatccta tagttgaaaa ggtgttacct
    14821 tttggagact atagtgagga agatattctc agaatcagtg cctgtcttga ggaacacatt
    14881 tatcatccta ttgctaatgc catcgtcaag caagctgaga tagagggaat tgaacatgag
    14941 gaaatgcatg ggaaactcca atatatcgca agcaagggga tcaaatctca tatagatggc
    15001 caaccagttc ttattgggaa ttatgtcttg atgcaggatg agcagattca tatcagttca
    15061 gaacaaaatg ctttaattga agagtacaag agtcactaca atctcttatt cttggcttat
    15121 caaaatgaat tgattggaat gttctgcatt catactcctt tgagaaaaga agcaaaaaca
    15181 gccttggata aacttaaggc acaggggaaa aaattgattc tggcaacagg agacaccttg
    15241 attaggacag aggaattagt caaagatttg ccctttgatc aggtctatac agacttgaaa
    15301 cctgatggga aatttgagtt agtagagaaa ctgcagaaag caggtcacac tattttgatg
    15361 gttggagatg gattgaatga ctcagcggct ttaactctat cagatatcgg tgtggtgatg
    15421 aatgagagtg cagatatttc taagcagatg agtgatatct tattgttaga taatcgtttg
    15481 gatttcttcc aagagttgga ttcgttatca tcatctttgc aaacactcat caagaaaaat
    15541 attcaggata ccgttgtcgt aaatagtagt ttgattggct ttggcttatt taattggttc
    15601 agtccttcaa atctctctat cttacataat ctaacaacct tgcgcattgt cctgcgtagc
    15661 ctgtctatta aaaatagata gatgagagtt attaacagca aaaaggagtt acctttctcg
    15721 aggttaactc ctttgtttat agataaatgt tgaatagttt agtaagtcaa tacaaagtat
    15781 tttttcttcc cacgacggat aacagtcagt tcattttcta acttatcagc gtcactcaag
    15841 acatagtcaa ggtcttggat gcggtcgccg tttacgtaga tggctccgtt ttggacgtct
    15901 tcacgggctt ggcgttttga gttaactata ccagatgaga cgagcagttc cacgatattg
    15961 ttgttttcgt ctgcctgtac ttggtagttt ggcacaccac gaagtccttg tttgagctct
    16021 ttgacagaaa ggtttttgat gtttcctgca aagagttgct cagtgatgtt aagtgcttct
    16081 ttgtaggctt cttctccgtg aacaagtgta acaacttcac gagccaagac tttttgagcc
    16141 aagcgttcgt gtggcgctgc ttcaaattgt ttacgaatat cttcaatctc atcaagtgac
    16201 aagaaagtaa agactttcaa gaagcgaaca gcgtcagcgt ccatcacgtt catccagaat
    16261 tggtacattt cgtatggaga agtcttttcg ggattgagcc agacggcatt tccttctgat
    16321 ttaccaaatt tcttaccagt tgcatctgtg attagtggaa cagtgataac gtgaccagtc
    16381 ttgtccgcct tacgacgaag caattcggta ccagctgtca tatttcccca ctggtcagaa
    16441 ccaccgattt gaagagtgac attatggtct tggttaagga cgaagaagtc gtacccttgc
    16501 atgatttggt aagcgaactc agtgtaagaa attcctgttt cgatccgttt tttaacagat
    16561 tccttactca tcatgtagtt gaccgtgaag tattttccaa tatcacggag gaagtcaatg
    16621 aagctgatgc tgccaaacca gtcgtagttg ttgaccatga cagccttgtt ttcgccattt
    16681 tcaaagtcaa gaaaacgaga aagttgtcct tggatagact tgacccagcc atctactgtg
    16741 tcttttgttt ggagactacg ttcagcatct ttgaaggacg gatctccgat gagacctgta
    16801 gcaccgccaa cgagcgcata aggtttgtga cctgctagtt gcaagcgacg acttgtcaag
    16861 attgcgacaa ggtggcctag gtgaaggctg tcagcagttg gatcgtagcc agtataataa
    16921 gaaacttgac cttcttctag ggctttacgc aaagcttctt catcagtcgt ttgaaatatc
    16981 aaaccacgct cttttagctc atcaaaaatg tgcatgtgtc ttttctcctt tttaaaatta
    17041 ttgtttctac ctattgtatc acaaacttgg gcaatagcct agcagaatag ggaagaaagt
    17101 ggctatttta ttgaaaattt cccttttgtg gtataataga tagagtgagg aaatccatgc
    17161 aaaatcaatt aaatgaatta aaacgaaaaa tgctggaatt tttccagcaa aaacaaaaaa
    17221 ataaaaaatc agctagacct ggcaagaaag gttcaagtac caaaaaatct aaaaccttag
    17281 ataagtcagc cattttccca gctattttac tgagtataaa agccttattt aacttactct
    17341 ttgtactcgg ttttctagga ggaatgttgg gagctgggat tgctttggga tacggagtgg
    17401 ccttatttga caaggttcgg gtgcctcaga cagaagaatt ggtgaatcag gtcaaggaca
    17461 tctcttctat ttcagagatt acctattcgg acgggacggt gattgcttcc atagagagtg
    17521 atttgttgcg cacttctatc tcatctgagc aaatttcgga aaatctgaag aaggctatca
    17581 ttgcgacaga agatgaacac tttaaagaac ataagggtgt agtacccaag gcggtgattc
    17641 gtgcgacctt ggggaaattt gtaggtttgg gttcctctag tgggggttca accttgaccc
    17701 agcaactaat taaacagcag gtggttgggg atgcgccgac cttggctcgt aaggcggcag
    17761 agattgtgga tgctcttgcc ttggaacgcg ccatgaataa agatgagatt ttaacgacct
    17821 atctcaatgt ggctcccttt ggccgaaata ataagggaca gaatattgca ggggctcggc
    17881 aagcagctga gggaattttc ggtgtagatg ccagtcagtt gactgttcct caagcagcat
    17941 ttttagcagg acttccacag agtcccatta cttactctcc ttatgaaaat actggggagt
    18001 tgaagagtga tgaagaccta gaaattggct taagacgggc taaggcagtt ctttacagta
    18061 tgtatcgtac aggtgcatta agcaaagacg agtattctca gtacaaggat tatgacctta
    18121 aacaggactt tttaccatcg ggcacggttg caggaatttc acgagactat ttatacttta
    18181 caactttggc agaagctcaa gaacgtatgt atgattatct agctcagaga gacaatgtct
    18241 ccgctaagga gttgaaaaat gaggcaactc agaagtttta tcgagatttg gcagccaagg
    18301 aaattgaaaa tggtggttat aagattacta ctaccataga tcagaaaatt cattctgcca
    18361 tgcaaagtgc ggttgctgat tatggctatc ttttagacga tggaacaggt cgtgtagaag
    18421 tagggaatgt cttgatggac aaccaaacag gtgctattct aggctttgta ggtggtcgta
    18481 attatcaaga aaatcaaaat aatcatgcct ttgataccaa acgttcgcca gcttctacta
    18541 ccaagccctt gctggcctac ggtattgcta ttgaccaggg cttgatggga agtgaaacga
    18601 ttctatctaa ctatccaaca aactttgcta atggcaatcc gattatgtat gctaatagca
    18661 agggaacagg aatgatgacc ttgggagaag ctctgaacta ttcatggaat atccctgctt
    18721 actggaccta tcgtatgctc cgtgaaaagg gtgttgatgt caagggttat atggaaaaga
    18781 tgggttacga gattcctgag tacggtattg agagcttgcc aatgggtggt ggtattgaag
    18841 tcacagttgc ccagcatacc aatggctatc agaccttagc taataatgga gtttatcatc
    18901 agaagcatgt gatttcaaag attgaagcag cagatggtag agtggtgtat gagtatcagg
    18961 ataaaccggt tcaagtctat tcaaaagcta ctgcgacgat tatgcaggga ttgctacgag
    19021 aagttctatc ctctcgtgtg acaacaacct tcaagtctaa cctgacttct ttaaatctta
    19081 ctctggctaa tgcagattgg attgggaaga ctggtacaac caaccaagac gaaaatatgt
    19141 ggctcatgct ttcgacacct agattaaccc taggtggctg gattgggcat gatgataatc
    19201 attcattgtc acgtagagca ggttattcta ataactctaa ttacatggct catctggtaa
    19261 atgcgattca gcaagcttcc ccaagcattt gggggaacga gcgctttgct ttagatccta
    19321 gtgtagtgaa atcggaagtc ttgaaatcaa caggtcaaaa accagggaag gtttctgttg
    19381 aaggaaaaga agtagaggtc acaggttcga ctgttaccag ctattgggct aataagtcag
    19441 gagcgctagc gacaagttat cgctttgcta ttggcggaag tgatgcggat tatcagaatg
    19501 cttggtctag tattgtgggg agtctaccaa ctccatccag ctccagcagt tcaagtagta
    19561 gttctagcga tagcagtaac tcaagtacta cacgaccttc ttcttcaagg gcgagacgat
    19621 aattctctaa atgaagtggc caatcaatgg attgccactt ttttcctttc gtgttacaat
    19681 aagggtatga atcagtatca gaaaaagatt gttaacggaa aaatttattc gctcctatcc
    19741 ggcttaatat ggggaatctg tggaatttta ggagagtact tctttactca ttatcaggtg
    19801 tcttcgggct ggattacctc tatgcgtttg acactggcag ggagtcttgt actcatttgg
    19861 tctgcaatac aattaaaatc gcaagtgcta gatatttggc gagacaagaa aaattacctg
    19921 ccctttttag cttatgctat tttggggatt ttttcagtcc agtatttttt ctatctctgt
    19981 gtagaatact caaatgctac gacagcaact attttacagt ttattagccc tgtctttatc
    20041 ctcttttaca atcgcttggt ttatcaaaaa cgagcgtcaa aaagcgctgt tttctatgtt
    20101 ttggttgcca tgctgggtgt ttgcttgatg gcgacaaagg gagatctctc tcagttatcc
    20161 atgacgccgc tagctcttat aacaggtttg ctgagtgcca tgggtgttat gtttaatgtt
    20221 atcttgcccc aaccttttgc taagcgttat ggttttgttc ctacggttgg gtgggggatg
    20281 attttggcag gtttgtttag caatgtcctc tcgccggttt atcagctttc ctttactctt
    20341 gatatttgga gtatcttgat ttgcctcatt atcgctttct ttggaacggc ttttgctttt
    20401 ttcatttcca tgaaggctgt gtccttggtt tctcctttgg tggttgccgt tatcagtgcc
    20461 agtgaacctc tctcttctgc tctcttgagt gttttgttct taggattagt agtggattgg
    20521 tccctccttc tagctatagc cttgattatt ttacccatga tatttttgtc tatagaagaa
    20581 gcgaaagaaa gtagataaaa agtcttttct taattctaaa agtgtgctat actagaatag
    20641 tcaataaaac acggggagat agttgtgaag agtgttttta ggttgtatcg gtaggggctt
    20701 gcttttaccg acagcacgtt ttatctcaca tccctaaaaa atcataccta aaaacagaca
    20761 aaaaggtttc aaatttgagg ccttttttgg tagaataggt atcattataa cgaaccagga
    20821 ggcacctatg actgctacaa aaatgaacgc tcaagaaatt atccaattta tcgccaatgc
    20881 tgaaaagaaa accagtgtca aagtaacctt tgaggggcaa ctcgcaactg ctgtgcctag
    20941 ctctgttgtc aaactaggaa atgtcctatt cggagactgg aaggacgtgg ctccgcttct
    21001 tgaaggtttg gtagaaaatc aagattatgt tgtcgagcaa gatgctcgta attctgcagt
    21061 tcctttgcta gataagcgtg ctatcaacgc tcgtatcgag ccaggtgcta ttatccgtga
    21121 ccaggtggaa attggtgaca atgctgttat catgatggga gctgttatca atatcggtgc
    21181 tgaaatcggt gctggaacca tgattgacat gggtgccatc cttggtggcc gtgctatcgt
    21241 tggaaaaaat agccacgttg gtgcaggtgc agttttggca ggtgtgattg agccagctag
    21301 tgctgaacca gtctgtgtcg gagacaatgt tcttatcggg gctaatgcag tggttatcga
    21361 aggagtccaa atcggtagtg gttcagttgt cgctgcagga gctatcgtta cccaagatgt
    21421 cccagaaaac gtggtagtag caggtgttcc agctcgtatt atcaaagaaa ttgatgccca
    21481 aactcaacaa aaaacagcgc tagaggatgc gcttcgtacc ttgtaattgt aaaagtaaaa
    21541 aagaggcgga accctttttc cagcctcttt ctgctatatc ggaggacaga tagatgttag
    21601 atttgattca gactagacga gctttacacc agattccaga gattggcttg gaggagttca
    21661 agactcaggc ttatttgctg gatgtgattg agaaattgac tacgggcaag gattttgttc
    21721 aaattcgtac ttggcggaca gggattttgg tctacctgca gggaagtcag ccggagcgaa
    21781 ccattggttg gcggacagat attgatggcc tgcctatcgt cgaacaaaca ggtctacctt
    21841 ttacttctca acaccaaggt cgcatgcatg cctgtggtca tgattttcat atgaccattg
    21901 ccttaggctg tcttgagcgc gcccttgagg agcaacccaa gaataatctg ctctttctat
    21961 ttcagcctgc tgaagaaaat gaagctggtg gtatgctcat gtatgaggac ggtgcttttg
    22021 gagattggtt gccaaaccaa ttctatggtc tccatgttcg tccagatttg aaagtcggcc
    22081 agattgcgac taatactcat acactctttg caggaacttg cgaggtgaag atccgtttca
    22141 aaggtaaagg tggccacgca gcttttcctc atgaagccaa tgacgccttg gtggcggcta
    22201 gttactttgt gacccaagtg cagtcagttg ttagccgcaa tgtcaaccca atcgagggag
    22261 cagtggtgac cttcggcgtt tttcaagctg gaaccaccaa caatgtcatc acagacacag
    22321 cttttttaca tggaactatt cgtgccttga cacaggacat gagtctcttg gtgcaaaaga
    22381 gggtaaaaac agttgcagaa ggggttgcag ctgcctttga tatggaagtc gaagtggaac
    22441 tcaaacaagg tggataccta cctgttgaga acaatccagc cttggcgcgt gaactgatgg
    22501 acttctttga cgagaaagac ggaatcgagt tgattgatat cgaacctgct atgacaggcg
    22561 aggactttgg ttatctcctt tcaaaggttg atggcgtgat gttttggcta ggtatcgata
    22621 gtccctacgc tcttcatcac cctcaaatga gtcctaagga agaagtctta gctattgggg
    22681 tggctgcggt gtctagtttc ctgaaaaaga aggcagcaga atagagggct tgtctatgaa
    22741 atcggaatta cgcaagcaag tcttgcatga aatgaaggct atatctcaag agcaaaaaca
    22801 ggctatagac caagctttaa ccgagcgaat tttacaacac cccttttatc aagaagccaa
    22861 ggtcatcgca acctacctct ctttttctca tgagtttcaa acgcgggaac tgattgagca
    22921 ggcgctgaag gacggcaaga aggttttgac acctaagacc tatcccaagg ggcgcatgga
    22981 ttttgtggtc tatgatccgc aacagttggt aaaaacttcc tttgccttac tggagccaca
    23041 gggagatttg gaagtagtgg atgcatctca gattgatttg attcatgttc ctggcctggc
    23101 ttttacgacg gatggatatc ggattggata tggtggaggt tattatgacc gctatctgga
    23161 acatttttct ggtcgtgctt tgagtacgat atatccttgt caaattcagg actttatacc
    23221 tgaaaaccat gatattcctg ttcaggaggt attaattgat gaaggaaatc tttgatagac
    23281 gttaccctgt gacgagtttc ttcctcttag tgacggcctt ggtattttta ctaatgttgg
    23341 tcactgcagg cggaaacttt gacagggcag atacattatt tcgatttgga gccatgtatg
    23401 ggccagctat tcgcctcttt cccgagcagg tttggcgtct cttgtctgcc atttttgttc
    23461 atattgggtg ggaacatttc attgttaata tgctttcact ttattatctt ggaaggcagg
    23521 tagaggagat tttcggttct aagcagtttt tctttctcta tcttttatca ggaatgatgg
    23581 gcaatctctt tgtttttgta tttagtccta aatccttagc agcaggcgcc tctacctctc
    23641 tttatgggct atttgccgcg attattgttc ttcgctatgc aactcgcaat ccttatattc
    23701 aacagctagg gcaatcctat ctgacacttt ttgtagttaa cattattgga agtgttctga
    23761 ttccaggaat cagcctagca ggccatatcg gtggtgcagt tggtggcgca tttctagcag
    23821 ttatctttcc agttagagga gaaaaacgga tgtataatac cagccagaga ttaggagcgg
    23881 tagtcttgtt cgtaggactc gccattttgc ttttctacaa gggaatggga atgtgacgaa
    23941 gttgaaagaa aatttgtgta atgaaaaaag ataaggcatt ttttgaacct tatcttttta
    24001 ttttatttta ttccttctca gtcaattctt ttccaagttg gatgaggtaa ttcttcaaat
    24061 catctttgac ttgtgggtgt ttgagggcgt agtcgatgga tgttttcatg aagccaaact
    24121 tgtctccgac atcgtaacga gccccattga actcacgagc aaatacacgt tgtgttttat
    24181 tgagggtgtc gattgcatct gtcagctgaa tttcatttcc tgcacctgga gcttggtttt
    24241 cgaggatttg gaaaatttca ggcgtgagga ggtagcgtcc gataatagca aggtcgctag
    24301 gagcgtcctc tggagctggt ttttcaacaa aggtttcaac actgtaaaga ccatcttttc
    24361 cttcgccttg cggagcaata accccgtaag cagatacttc gtcatgaggg actggcatga
    24421 cagcgatagt agacgcgtgg gtacgctcgt agtcatccat gagttgtttg gtaagtggaa
    24481 cagccttttc gtctgtgata tccatcaagt catcaccaag cataacgaca aaaggttcat
    24541 ttccgacgaa agccttagct tgcaaaacag catctccgag accgcgtgga tgagtttggc
    24601 ggataaaatg cagacgcatg tcagttgttt tatcaactag cttcaaaaga tctgttttcc
    24661 ctttttcttt gaggttatat tccaattcga agtttgaatc aaagtggtcc tcaatagaac
    24721 gttttgattt accagtgaca actagaatat cttcaatacc tgatttgaga gcctcttcca
    24781 cgataaactg gatagttggt ttgtctacga ttggcaacat ttctttggca agggccttgg
    24841 ttgctggtaa aaatcgagtt cctagtccag cagcagggat gactgccttt ctaacttttg
    24901 atgtcataaa aatcctttct atagagggtt aagaccactc attttctgct ttaaattcat
    24961 tgttcatgat gtcataaatg gcatctttga tattggttcc gtgataaata acttggtaaa
    25021 tagcctgtgt aatgggcata tagactccaa gttcttgggc tagttcatag gctgctcgag
    25081 tcgttgaaat tccttcgatt accatgccca tattagcttc tatatcagct agggattctc
    25141 ctcgtccgag agcatctcca gctctccagt tacgagagtg gatggaagtt cccgttacga
    25201 tcaaatctcc cacaccagat aagccgctat aggtcaatgg actggccccg agtgctaccc
    25261 ctaggcgggt gatttctgct aaacctcgag cgatgatggc tgccttagca ttatcaccaa
    25321 atccaagacc atgtaaagct ccagcaccga cagcaataat atttttaaga gcaccagcag
    25381 tttcaacccc gataacatcc gtattggtat aaagtcggaa gtagtgatta ctaaatagct
    25441 cctgaacgta ttgagctgtt tgtaaatctt tagaagcagc agtgattaaa gttaggtcac
    25501 gcacaatggt ttcttccgca tgactaggcc ctgaaacaac gacaatatca ctgcggagct
    25561 gttcaggaat ttcttcttca aggatagttg ataatcgttt atggctatca ggttctaatc
    25621 cctttgaagc gtgcatgatg atagccttat ggtccaaggt ttgtgcaact tgttgggcaa
    25681 caagtcgtgt cacttttgtt gggacaacaa acaaaatcgc atccacatct ttcaatgttt
    25741 ctgctaagtc ggtgtaggca atgatatttt catctagaac gacatcttta aagtagtgct
    25801 tgttagtatg ataggtatta atttcattga tttgctcggg aagatttccc caaatacgta
    25861 cctcgtgtcc attgtcattt aagacttgtg aaagggcagt tccccaagaa ccaggcccca
    25921 agacggcgac ggtttgtttt tccatgattt cctccttgat agagttcttt ccattattct
    25981 atcatattct agaggatttt gcacttgcat tactggggta tggtggaaaa atacacaaag
    26041 accgagatga agggataaac tcttagtctt tagttaaagt gagaagaaat ctgatagata
    26101 gtttgattca gtatatgcta taaatagagg taaacaactc aggaggttct tgtaggttgc
    26161 gagagtttgg cgaaaaaatt aaaagattac gtttggctaa aaaaatcagt cgttcagaat
    26221 tttgtggtga tgagtctgaa ttaagtatcc gtcaattaat tagaattgaa aatggagaat
    26281 ccagaccaac actaacaaag ttaaaatata tcgctgaacg ttttgggggg gggtgaagat
    26341 tacaagttga tgctaagtta tatagagttg gataaggaat acctagaatt gaagtatttc
    26401 ttgatgagga ctcctacata cgaagatgaa actatcgccc aaaagaaaga gagtgttttt
    26461 gctaagattt ttgaagagta ttatgatagg ctacctgagg aagaaagatt tatcatccca
    26521 aattattcat atctagcact agcgaactac acagttcaaa aattaccaga aaagctagtt
    26581 gaaatactgt ccttctggtg attttgctta atttctgatg ttcgagacat gattgaggag
    26641 atttgtcttg attttttcct aaaaaagggc agactcctcc cttggtttga tgctcgattt
    26701 ttaaaataag gtgacttatt ctgttaggca tgttttcccc tgccctctag ctctatatgg
    26761 aacagggagt atcagattta tctggcagat tgtatcaaat aaggcttgaa actgttttga
    26821 ataggcgtat agacttgaga atttgagaat atgtcgtcta gcagtagaga catattttcc
    26881 ggcaatatga aggaagagac gtcgaaaacg cttgatagtc aaggacttta cttcatcacc
    26941 agctagctgc tttaaaaaga ggtagagatt ataagccaga cagcccatca tcatacgtac
    27001 ttcattctta atcatggtcg aactatctgt cttatcccca aagaatcctg ctttcctttc
    27061 tttgataaag ttttcagcat tccctcgttc acgatagaat tgaaacagct cctcactatc
    27121 tacactgctt aaattgttca aatgaacttt tttacaacca taaaaaagag cacttaatcg
    27181 tactcttctc ttttaaggat tagttgaatt aattcaaatc cactagttct cctgtttcta
    27241 ggtagactag gcgttcacaa atgttagcaa tgtaatcagc gaagcgttcc agatgtccta
    27301 tgatataaag gtattgagtt ccattgggaa ttgaggtttc ttggtctttc ataagtccaa
    27361 tgatttcctt tgataaggca taataatatt ggtcaatctg ttcatctttt tgagcaatac
    27421 taatagcttt tgaggcttgg tgcaaaggaa aggcaaccaa taaatcggct agcatgctga
    27481 gggataattt acccatttgg tgtaactgtt cttcgtcagg ggctagttga ttttctttta
    27541 gttgcaaaac agctttggca atgcctgcca tatggtctcc catacgttca aggtctgaac
    27601 aagaagacat gatgctaatc acaaatcgaa ggtcagacac ttgtggctgt tgcaaggcca
    27661 acaaacgggc acaggtcaat tcgatagcgc tttgaccttg gttgatagca tgatccttat
    27721 tgataattag ctctgccatc tccttgtctt tggaggctaa ggccagtaag gcttttgaag
    27781 ctgtttcaag gacaagttgc cctagtccta aaaaggattg ttctaattca tgcaattcta
    27841 agtcaaattg atttctcata ggtttttcct tccttatccg aaccgtcctg aaatatagtc
    27901 ttctgtgcgc tgatcttttg gattggtaaa cacgtcaacg gtatctccaa attcgcaaat
    27961 ttctcctgtt aagaaaaaag cagttttatc tgaaatacgt gaagcttgtt gcatgttatg
    28021 ggtaacaatg ataatcgtat aatccttttt tagttgctga atgaggtctt caatttttaa
    28081 agtggagata gggtctaagg ctgaagtcgg ctcatccatt aacagaacat caggttctac
    28141 tgctaaagct cgcgcaatgc aaaggcgttg ctgctgaccg ccagataagg acatggcact
    28201 ctttttaaga tcatctttga cttcttccca aatggctgcc ccttttaaag atttctccac
    28261 taaggcatct aattgttttt tgtctcgaat accatgtgtc cttggaccat aagccacgtt
    28321 atcatagata gacatggcaa agggattagg ctgttgaaaa accatcccta cacgcttgcg
    28381 tagctgatta aggttgaatt tgctactata aatatcttgc tcatctaaga ggacttggcc
    28441 ttcaatatgg caagaaggaa ccaaatcgtt catccggtta agggttttta gaaaagttga
    28501 tttgccacaa ccagatgggc ctatcaaggc agtaatctgt ctttctggta attgaatcga
    28561 aatatttttt aaggcttgaa aatccccgta aaataagtct aggtgtctga ctgaaaatgt
    28621 tcccatacta ggaagctcct ttcacaagtt ttcgagataa taagcttgat agagtattta
    28681 tcattaaaac agtaataatc aaaatcacac cggtagcata ggcttcattg acatgtagcc
    28741 cctcacttga cagcatatac atatgcaggg ctagagaacg acctgaagac atgagactac
    28801 ttggcgtatt ggtagaggta cctaatgtat acatgagggc agctgtctca ccaacgatac
    28861 ggccaatagc tagtatcact ccagctaaaa tacctggcat ggcaactggt agaacaattc
    28921 taaaaacagt ccgtaactta cctgccccaa gtccataact tgcttgacgc atgctatcac
    28981 taacagataa aagggcttct tctgttgagc gaataatgac tggcaacacc atgatgactg
    29041 aggttaagat tcctgataac agagagtatt gaaaacctaa gaagactaca aagaagagca
    29101 tgccaaacag accaaaaaca atggaaggaa tcccagacaa ggtatctgag gccaatcgca
    29161 tgattttaac acaaagggaa tctttttttg tatattccac aagataaaaa ccagcaaaaa
    29221 tccctatggg caaggctaaa agaagagcac caaagaccag aataacggtg gaaataatcg
    29281 ctggcataag ggaaatgttc tcagaagtat aagtccaaga aaagagggat agacttagat
    29341 gaggtaagcc tttgatgagg ataaaaccaa tgattaaaaa gagagagcca aaggttaaag
    29401 ctgaaaaaca ataaacgaga agttttagca ggtatttact cataagatga ttttcctttc
    29461 aagtaggcaa agtaggcatt aatcaagaga ataaggaaaa agagaactgc tgaggttgca
    29521 ataagggctt ccctatgctg acctgatgcg taagccattt ccagaacaat attggttgtt
    29581 aaggttctgg ttcctgaaaa gagtccactt ggaataatcg gctggttgcc tgccaccaaa
    29641 ataactgcca tggtttcacc tactgcgcga ccgattccta aaataactgc tgataaaata
    29701 ccagatctcg cagctggcaa gatgacacta aaaatactcc gttcatgact agctcctaga
    29761 gccaagctac cagaataata cgttttggga actgttcgga tagcagattc tgacaaactg
    29821 ataatggttg gcaaaatcat tattcctaat agtaacgaag cggttaggac actcatgcca
    29881 tttcctaaaa aggttctaat ccaaggcacc aataattgta ggccgaaaaa accataaaca
    29941 atagatggaa tggccgccat caagttgata gctgatttta agaagccata gacgggcttt
    30001 ggacaataat aaaccataaa caccgatgtc aagatgcctg ttggcacccc aatcacaatc
    30061 gctcctaagg taattaataa ggaaccaacg atcattggta aaataccata gcttgccgga
    30121 atgttcgttg gcgaccaatc actgcctaat aaaaaacggg caaagccgta gttagccatg
    30181 aaaggtaagc cattactaaa aataaagaaa cagattagca aaatagctac aacagctact
    30241 gttgcactca tgaaaaaaat tgccctaaaa actgcttctt tgaaggcttg ttttgtcaca
    30301 tcttgtcctt tctagtgaag aaagtaaggg agatacgaca cctccctact tgccttcttt
    30361 atcttattgt acgatgaaac gtctgcatct ctttagagat ttatggagca aacattttat
    30421 ttaatcttgt cccaggtggt taatttgcca ctaaaaacgt ctgcaagttc agccatactg
    30481 acttggcttg ccttattgtc attattgacc acaacagcaa taccgtctaa agcaatagca
    30541 tcatgggtga gactcttacc ttcttcagga gttaattccc tagaaaccat accaatatca
    30601 gcggttttct ccttaacagc ggtaatacct gctgaagacc cattagaggt aatatcaatc
    30661 gtaacttctg gattttcttt tttataagct tctgctaatt tttccattaa agaagatact
    30721 gaagtggaac ctacaacaga caacttgcct gataagtgtt ggcttgtata ttctgtggtt
    30781 tcggttttag cttcaataaa tttattatct gtgaccactt gttgaccttg tttggagtgg
    30841 ataaagctga taaaatcttg acctagcttg gaaagattag aagaccaaac aatgttgaag
    30901 ggacgttgaa gagggtattc accatctaaa actgtgtctc gactagcctt gacaccatca
    30961 atctctaaag ccttgacaga tttcgttaaa gatcccaagg agatgtagcc gatagcatta
    31021 gcattccctt gaactgctga gagaacacct tctgtactat tttgaatcac agctgttttg
    31081 gcagtgttgt caattttttt atcaccgtct tttttgagaa tccctgtgat ttctgtgaag
    31141 gcaccccgtg ttccagagcc attttctcgt gaaatcacct caatcgttcc tgaagctgac
    31201 tgtttggaag cagctgactg attgccacag gcaacaagcc caaatcctga taagccaatg
    31261 gctgcaagag taagcatttt tttgaatttc ataataatca cctttatctc tatgtatttt
    31321 tcttgtgtag gcttactaca tttatagtct aacaagtctt tgtaaaggtt tatccctgat
    31381 tcatgtaaag attgtgtaaa gaatcaaaaa aagccacttt tgaaaaatgg ctgctcctaa
    31441 aaatagcttt aaaaattatt agtcctgtgc gaaagattgg ttaggaagaa aaatcgtgaa
    31501 gcaactgcct ctgccaagct gactcgtcac cgtgacttgg ccacctaata attgactgag
    31561 ttctttgaca atggcaaggc caagaccagt gccaccagtt tgtctgcttc gacctttatt
    31621 aactcggtaa aaacgttcaa aaatacgatc ctgctctaat tgactaatac caatccctgt
    31681 atctgataca gaaatcttaa tgccttcgtt caccttttgg gtcttgacct caatttttcc
    31741 cccttgttca gtgtaacgga tggcattgga taaaagattg agtaagattt gggaaagtaa
    31801 ttgactatct gatacgaggg tgacatcatc tggcacctgc acctttagct gtaaatcctt
    31861 cttcttgagc tgaggttgca agctttgagt caaatcctgt acaaattctg ccaaagaaag
    31921 ggtcgtccat tgtataggca tttgttgagc cttagataag gtaagaagat gctcaacaat
    31981 atgctcaaga cgcaagcttt ctttgtaaat aatgtctaga aagtcatcct tgagcgcttc
    32041 ttcttcagct gacatcccct taatggtttc agcaaagccc ttaatcgaag taactggtgt
    32101 cctcaattca tgggaggcat ttgagacaaa ggctaaattt aacttttcat aagttctaat
    32161 cgttgttaaa tcatatagca agacgagcac agcttccaca gattgggtgg ggctaaaaac
    32221 gggaactgct gtcacttcta aaatcaagtc accctcatga aacccactta cttcttgttt
    32281 taaccttgtt ttttgatcaa aggcttggtg aactaaattt cgaatatcca tccgtttgag
    32341 gtcatcaagt gaacttatgt cgccgtccac atcgggaaaa taatgaggca gagagcgact
    32401 ggataataac atctgacctt gagcggaaac taaaaacgtc cccatggtta ggtgcgacag
    32461 aagaacctcc attgtttcgg ctaggtcctt gtattgctga tcctgttggg agactttggt
    32521 ttttagggca gacacatact gagccaaaga ctttaagtct tcttgccctt tttctaaaaa
    32581 gtattcacta ctggtcaaga gaggttggtg caaggtctca aaagcaactt cccatttcca
    32641 aaggcaaaag agccagtagc caccttgtcc caaagaaagg gctagaagaa agagaccgat
    32701 gcctttactg atccaagtta atgccatccc tgcaatcaga atgaggctaa cacttagatt
    32761 gactagccaa aattgaaggt agcgtttcat ctataactcc ttgaacttat aaccataacc
    32821 ccgaatggtt cgaataaatt gaggggcttt aggattgtct tcaatttttt ccctcaactt
    32881 accaatatga acgtccacca aacgtgtttc ctgcccaaag tcataccccc agatacgttc
    32941 caaaagacgc tctctagtca gtgtcatgtt gggatgtttc ataagataga gcaagagttc
    33001 aaattctttt ggggtcaaac tcagtaactt attcgccttg tagacttcat gacgctcagg
    33061 gtatactttc aaggtcccaa atagccaaga atcgtcagcg atattatctg aatcatctcc
    33121 ttcttgttct cctttagttc gcctgaggac agccttgaca cgcgccagca attctctagg
    33181 gctaaaaggc ttggtcaggt agtcatcagc ccctaattcc aaggccaaaa ccttatcaaa
    33241 ttcatcactt ttcgcagaaa ccatcataat tggagttttg acgcctttgg ctctcagccg
    33301 cttacaaact tccatgccat ctaattgtgg taacatgata tcaagcaaga taaaatcaaa
    33361 gggttctgtt tctgccaaag ctaaggcctt ccgtccattt gttaccaatt gagtagaaaa
    33421 gccttcctta cttaaatggt agtcaagcaa tttcagaatg tgttcttcat catccactaa
    33481 taagacttgt tttgtcatct attatctcct attggtaaca ttataacaca attatcagaa
    33541 atcctaacat tgctaaatca gattaaattt gcttatcaag actagtatct ggtcaaacgc
    33601 tcaatcatct ccttgtgctc tggataggtc gccagtagat ctaccctttc aaataattca
    33661 aaatcctcaa attcaaaacc aggagcaaca agacaagaaa ccagagcata atccttatca
    33721 actgttgatc cccaaatagt gcccttagga acacagtagt gaagttgttg ccctttggat
    33781 atgtccaggc ctaaagtgac tgcttcgtag tgaccatctg ctgtaatcat gtgaacagta
    33841 agtggggatc ctgcatgaaa ataccagatt tcatctgctg tcaatcggtg aaaatgtgaa
    33901 ggattcgttt cttctaataa gaaataaata ctggtataaa gcgcccttcc cttaccagca
    33961 aggtttatag tgtctgaagc tttttttgtt tgtctaaaat agccaccttc aacatgggga
    34021 gctaactcta gagttcttat caagtcttct ttatccgtcg gagccaatgg gttgaagtaa
    34081 ctcttgttca aaggtggtgt tacgatttca agaactcctc tcagctctga ggacacgata
    34141 atgattgatg cgacggaagt acaaatcaat cgccctaaaa aaagaattag cgaattattc
    34201 tgg
//
LOCUS       DAVBCN010000014        33324 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVBCN010000014 DAVBCN010000000
VERSION     DAVBCN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33324
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.014"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..327
                     /locus_tag="VQ497_000630"
     CDS             <1..327
                     /locus_tag="VQ497_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET2516977.1"
                     /translation="KPQTEKPEEETPREEKPQSEKPESPKPTEEPEEESPEESEEPQV
                     ETEKVEEKMREAEDLLGKIQDPIIKSNAKETLTGLKNNLLFGTQDNNTIMAEAEKLLA
                     LLKESK"
     gene            749..979
                     /locus_tag="VQ497_000631"
     CDS             749..979
                     /locus_tag="VQ497_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HET2516978.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAIKC"
     gene            1383..2096
                     /locus_tag="VQ497_000632"
     CDS             1383..2096
                     /locus_tag="VQ497_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HET2516979.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            2154..2756
                     /locus_tag="VQ497_000633"
     CDS             2154..2756
                     /locus_tag="VQ497_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516980.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCMGDFPHDFF
                     DIYLDHVAKYAYEQKVNNIKEYYPLKRAILHQENALYFRFFSNFDDFLEKNYLKTIWQ
                     VSKETPFSEMDFNMFKNISEKIIFERGSKMLNDLKSNYKK"
     gene            2892..3545
                     /locus_tag="VQ497_000634"
     CDS             2892..3545
                     /locus_tag="VQ497_000634"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET2516981.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            3555..4019
                     /locus_tag="VQ497_000635"
     CDS             3555..4019
                     /locus_tag="VQ497_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET2516982.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            4032..5300
                     /locus_tag="VQ497_000636"
     CDS             4032..5300
                     /locus_tag="VQ497_000636"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET2516983.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            5314..5574
                     /locus_tag="VQ497_000637"
     CDS             5314..5574
                     /locus_tag="VQ497_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516984.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            5585..6946
                     /gene="pdrM"
                     /locus_tag="VQ497_000638"
     CDS             5585..6946
                     /gene="pdrM"
                     /locus_tag="VQ497_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HET2516985.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            7100..8068
                     /locus_tag="VQ497_000639"
     CDS             7100..8068
                     /locus_tag="VQ497_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET2516986.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            8084..9076
                     /locus_tag="VQ497_000640"
     CDS             8084..9076
                     /locus_tag="VQ497_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET2516987.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDKNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYISEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            9294..10337
                     /locus_tag="VQ497_000641"
     CDS             9294..10337
                     /locus_tag="VQ497_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HET2516988.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            10401..12086
                     /gene="lpdA"
                     /locus_tag="VQ497_000642"
     CDS             10401..12086
                     /gene="lpdA"
                     /locus_tag="VQ497_000642"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET2516989.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEIIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMQSPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDAEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEIIGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            12150..13139
                     /locus_tag="VQ497_000643"
     CDS             12150..13139
                     /locus_tag="VQ497_000643"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HET2516990.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(13206..14276)
                     /gene="xerS"
                     /locus_tag="VQ497_000644"
     CDS             complement(13206..14276)
                     /gene="xerS"
                     /locus_tag="VQ497_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HET2516991.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
     gene            complement(14860..16410)
                     /locus_tag="VQ497_000645"
     CDS             complement(14860..16410)
                     /locus_tag="VQ497_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET2516992.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFIIIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGSLVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(16426..17205)
                     /locus_tag="VQ497_000646"
     CDS             complement(16426..17205)
                     /locus_tag="VQ497_000646"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET2516993.1"
                     /translation="MATIKEIKELLVTVKELESPIFLDLEKDNRSGVQKEISKHKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQVVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(17192..18043)
                     /gene="ylqF"
                     /locus_tag="VQ497_000647"
     CDS             complement(17192..18043)
                     /gene="ylqF"
                     /locus_tag="VQ497_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HET2516994.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            complement(18250..18426)
                     /locus_tag="VQ497_000648"
     CDS             complement(18250..18426)
                     /locus_tag="VQ497_000648"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2516995.1"
                     /translation="MQAGIPTDNEVSYALIYQQETLPATGSSTSALTALGLLAVGSLV
                     LLVHNMTGTVFCSL"
     gene            complement(18478..24369)
                     /gene="iga"
                     /locus_tag="VQ497_000649"
     CDS             complement(18478..24369)
                     /gene="iga"
                     /locus_tag="VQ497_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin A1 protease"
                     /protein_id="HET2516996.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGSIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVTDKG
                     ETEVQPESPDTVVSDKGEPEQVAPLPEYKGNIEQVKPETPVEKTKEQGPEKTEEVPVK
                     PTEETPVNPNEGTTEGTSIQEAENPVQPAEESTTNSEKVSPDTSSENTGEVSSNPSDS
                     TTSVGESNKPEHNDSKNENSEKTVEEVPVNPNEGTVEGTSNQETEKPVQPAEETQTNS
                     GKIANENTGEVSNKPSDSKPPVEESNQPEKNGTATKPENSGNTTSENGQTEPEKKLEL
                     RNVSDIELYSQTNGTYRQHVSLDGIPENTDTYFVKVKSSAFKDVYIPVASITEEKRNG
                     QSVYKITAKAEKLQQELENKYVDNFTFYLDKKAKEENTNFTSFSNLVKAINQNPSGTY
                     HLAASLNANEVELGPDERSYIKDTFTGRLIGEKDGKNYAIYNLKKPLFENLSGATVEK
                     LSLKNVAISGKNDIGSLANEATNGTKIKQVHVDGVLAGERGVGGLLAKADQSSIAESS
                     FKGRIVNTYETTDAYNIGGLVGHLTGKNASIAKSKATVTISSNTNRSDQTVGGLAGLV
                     DQDAHIQNSYAEGDINNVKHFGKVAGVAGYLWDRTSGEEKHAGELTNVLSDVNVTNGN
                     AITGYHYTGMKVANTFSSKANRVFNVTLEKDEVVSKESFEERGTMLDASQIVSKKAEI
                     NPLTLPTVEPLSTSGKKDSDFSKIAHYQANRALVYKNIEKLLPFYNKSTIVKYGNLVK
                     ENSLLYQKELLSAVMMKDDQVITDIVSNKQTANKLLLHYNDHSSEKFDLKYQTDFANL
                     AEYNLGNTGLLYTPNQFLYDRDSIVKEVLPELQKLDYQSDAIRKTLGISPEVKLTELY
                     LEDQFSKTKQNLGDSLKKLLSADAGLASDNSVTRGYLVDKIKNNKEALLLGLTYLERW
                     YNFNYGQVNVKDLVMYHPDFFGKGNTSPLDTLIELGKSGFNNLLAKNNVDTYGISLAS
                     QHGTTDLFSTLEHYRKVFLPNTSNNDWFKSETKAYIVEEKSTIEEVKTKQGLAGTKYS
                     IGVYDRITSATWKYRNMVLPLLTLPERSVFVISTMSSLGFGAYDRYRSSDHKAGKALN
                     DFVEENARETAKRQRDHYDYWYRILDEQSREKLYRTILLYDAYKFGDDTTSGKATAEA
                     KFDSSNPAMKNFFGPVGNKVVHNQHGAYATGDGVYYMSYRMLDKDGAITYTHEMTHDS
                     DQDIYLGGYGRRNGLGPEFFAKGLLQAPDQPSDATITINSILKHSKSDSTEGSRLQVL
                     DPTERFQNAADLQNYVHNMFDLIYMMEYLEGQSIVNKLSVYQKMAALRKIENKYVKDP
                     ADGNEVYATNVVKELTEAEARNLNSFESLIDHNILSAREYQSGDYERNGYYTIKLFAP
                     IYSALSSEKGTPGDLMGRRIAYELLAAKGFKDGMVPYISNQYEEDAKQQGQTINLYGK
                     ERGLVTDELVLKKVFDGKYKTWAEFKTAMYQERVDQFGNLKQVTFKDPTKPWPSYGTK
                     TINNVDELQALMDQAVLKDAEGPRWSNYDPEIDSAVHKLKRAIFKAYLDQTNDFRSSI
                     FENKK"
     gene            complement(24578..25756)
                     /locus_tag="VQ497_000650"
     CDS             complement(24578..25756)
                     /locus_tag="VQ497_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2516997.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCRNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLPLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWILNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            complement(25926..29576)
                     /gene="addA"
                     /locus_tag="VQ497_000651"
     CDS             complement(25926..29576)
                     /gene="addA"
                     /locus_tag="VQ497_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HET2516998.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEICSLNQESDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FAKLGQLSDQITILDYQERYHQDTWKLAKTFQTFMSHFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALVEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIDEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQIIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTALGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            complement(29573..32848)
                     /gene="rexB"
                     /locus_tag="VQ497_000652"
     CDS             complement(29573..32848)
                     /gene="rexB"
                     /locus_tag="VQ497_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HET2516999.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQTTEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPISPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            complement(33048..33203)
                     /locus_tag="VQ497_000653"
                     /pseudo
     CDS             complement(33048..33203)
                     /locus_tag="VQ497_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
BASE COUNT         9514 a         6539 c         6861 g        10410 t
ORIGIN      
        1 aaacctcaga cagaaaaacc tgaggaagaa acccctcgag aagagaaacc gcaaagcgag
       61 aaaccagagt ctccaaaacc aacagaggaa ccagaagaag aatcaccaga ggaatcagaa
      121 gaacctcagg tcgagactga aaaggttgaa gaaaaaatga gagaggctga agatttactt
      181 ggaaaaatcc aggatccaat tatcaagtcc aatgccaaag agactctcac aggattaaaa
      241 aataatttac tatttggcac ccaggacaac aatactatta tggcagaagc tgaaaaacta
      301 ttggctttat taaaggagag taagtaaagg tagcagcatt ttctaactcc taaaaacagg
      361 ataggagaac gggaaaacga aaaatgagag cagaatgtga gttctagttc tcattttttt
      421 catgaaaatg tgcaaaatat agtagattga aactagaata gtatacctct acttctaaaa
      481 cattgttaga aatcgatttg actgtcctgt tcttatttca ttttactata tcttaacaga
      541 tagtgtaaat aaagataaac tatttactgg ctaattaatc agttaaacac tagttaagga
      601 gtaatgatga aaaaaagaac aatactatta ttgatggcca gtctgttagc tcttgtctta
      661 ggagcatgtg gtttcttgga catattgatc ctggatcatt ctcatcagga ttactcttta
      721 ctgctatttt agaaactggg gtggtttgat ggaaagtatt ggtattggtc ttgttatcgt
      781 ttcacattcc aaacacattg cagaaggtgt tgttgaactg attagtaaag tagctaaaga
      841 tgttccgatt acttatgtag gaggaaccga gggcggagga attggaacga gttttgatca
      901 agtagatagg gttgtttccg aaaatccagc agatacttta cttgcctttt ttgacctagg
      961 ttctgctata aaatgttaag taaatgattt caaaatcaga aagggattgc tttgggcagt
     1021 tcctttttaa tgtaaatgta aaaggagtag aatcatagtg tttctaaatt gtgaatcaat
     1081 caaaactgat tgtgatgggg ctattctagc tttagaatcc ttcaaaaatt aaaatttaag
     1141 gcaatcaatt acttggaaga gtatgaaagc atttagttta taggaattct aggcctagaa
     1201 ttacatatgt atttatgaag ataggggtgt tcgatagtta gtattgttct gttctgaaag
     1261 atttgagctg tcagttgtat agaaagtgtt cgaatttttt ttaagtgatt aaattagtta
     1321 attgtatgag gttctttatg atataatgtt cttaatgaat tttcagaaag gaaaacctca
     1381 aattgttcta caaatttcta ctcttcgacc tcgaccacac tcttcttgat tttgatgctg
     1441 ctgaggatgt ggctttgacc caacttctaa aagaagaagg agttgcggat attcaggctt
     1501 ataaagatta ttacgttcct atgaacaagg ctctctggaa agacttggag ctgaagaaaa
     1561 tcagtaaaca agagctggtt aacacgcgct tttctcgttt atttgctcat tttggacagg
     1621 aaaaagacgg tagttttctt gcccagcgtt accaatttta cctcgcccag cagggacaaa
     1681 cactatcggg cgctcatgat ctcttggaca gcctcattga gcgtgattat aacttgtatg
     1741 ctgcgacaaa tggcattact gccattcaga caggacgttt ggctcaatct ggtctagcac
     1801 cttatttcaa tcaagtcttt atctcagaac agttgcaaac tcaaaagccg gatgctctct
     1861 tttatgaaaa gattggccag caaattgctg gatttagtaa agaaaagacg ctgatgattg
     1921 gagattctct aaccgccgac attcaaggtg gcaataatgc ggggattgat actatctggt
     1981 ataatcctca tcacctcgaa aatcacacac aagcccagcc gacttacgaa gtctattctt
     2041 accaagactt gctggattgt ttagataaaa atattcttga aaagatcaca ttttaaagga
     2101 gacgagctaa tgactacaaa aaagctaata ttactattga agagtacatt gaaatgtctg
     2161 aagttgattt taatgaagct gttaattatg aatttacatc tgacacttgt caattagcaa
     2221 atagtattta tcaatctctt tttaagtttt ttgataagaa aaatttctct ggcgatttaa
     2281 tttttacttg gaaatctcca tcattagtca aagaagggga ttatattggg agaagggatt
     2341 cacaagtaga taatcttaga gtaataggaa atatatttcc gaattatctt actaatcgaa
     2401 aatatagcct caatatgaat cgtaatggct gtatgggaga ttttcctcat gacttttttg
     2461 atatatacct agatcatgta gcaaaatatg cctacgaaca aaaagttaat aatattaaag
     2521 agtattatcc tttaaaaaga gcgattttac accaagagaa tgcattgtat tttcgatttt
     2581 tttctaattt tgacgacttt ttagaaaaaa attatttaaa gactatatgg caagtttcta
     2641 aagaaactcc tttttctgaa atggatttta atatgtttaa aaatatttca gaaaagataa
     2701 tcttcgaaag aggtagtaaa atgcttaatg atttaaaaag taattataaa aaatagccag
     2761 tctttataaa ctgtattcac gtattcaaat cgtatatagt cgatttatta aatctttaga
     2821 taaactgtaa aaagtggttt ccatagccac tttttgctat aatagaggca gtaaaaatga
     2881 aggagtcggc tatggaacac tcgtcttggc atgctttgat taaggcgcaa ttacctgagg
     2941 gttatttcgg gaaaatcaat cagtttatgg agcaggtcta ttctcagggg attatttatc
     3001 cacccaagga aaaggttttt caggctctct tgacaacact gcttgaagaa gttaaggtgg
     3061 taattctagg gcaagacccc tatcacggac caggtcaagc gcagggcttg agtttttctg
     3121 tacctgactc tatcccagct ccaccatcct tgcaaaatat cttgaaagaa ttgtcagatg
     3181 atatcggagt taagaaatct catgatttga cagcttgggc tgagcaagga gtcttgcttc
     3241 ttaatgcttg tttgactgtt cctgctggac aggccaatgg tcatgctggt caaatctggg
     3301 agccttttac tgatgctgtg attcaggtgg tcaatcatct agatagacca gtcgtttttg
     3361 tactctgggg agcttatgca cgtaagaaga aggccttagt taccaatcct catcacttga
     3421 ttatcgaatc agcccatcca agtcctttgt cggtttatag aggattttgg ggttccaagc
     3481 ccttttccaa ggccaatgca ttcttaaaag agacaggaca agagccaatc gattggctta
     3541 gataaggaga aaatatgcct cagttagcga cgatttgcta cattgataat gggaaagaac
     3601 tgctcatgct ccatcgtaat aagaaaccca atgatgtcca tgaagggaaa tggattggtg
     3661 tgggtggtaa gctagagaga ggagagacgc cccaggaatg cgcggcgcgt gaaatccttg
     3721 aagaaacagg gctcaaagcc aagccagttc taaaaggtgt catcactttt cctgaattta
     3781 caccagattt agactggtac acctatgttt ttaaggtgac ggagtttgag ggcgacttga
     3841 ttgactgcaa tgaggggacg ctagaatggg ttccctatga cgaggttttg agcaagccga
     3901 cttgggaagg tgaccacacc tttgttgagt ggcttttaga ggataaaccc ttcttttcag
     3961 ccaagtttgt ttatgatggg gataaattgt tggataccca agttgatttc tatgaataaa
     4021 ggagaaagca gatgctacta atcaaaaatg gtcgtgtaat ggatcctaag tctggtttgg
     4081 atcaagtttg tgatgtctta gttcaagatg ggaaaattat caaaattgcg cctgagatca
     4141 cggaagaagg agcagaaacg attgatgcta ctggtcttgt ggttgctcct ggtttggtcg
     4201 atattcatgt gcatttccgt gaacctggtc aaacacataa agaagacatt catactggtg
     4261 ccctggcagc cgctgcaggt ggttttacta ctgtcgtcat gatggctaat accagtccaa
     4321 ccatttcaga cgtggagact ttgcaagcag ttctccagtc agctgccaaa gagaagatta
     4381 atgtcaagac agttgcgacc attactaaaa actttaatgg taaaaacttg actgacttta
     4441 aggcgctctt agaagctggt gcggttggtt tctctgatga cggtattccg cttgagagca
     4501 gtaagattgt caaggaagcc atggaggaag ccaaaaaact caataccttt atcagccttc
     4561 atgaggaaga tccaggtttg aacggtgttc ttggctttaa tgaaaatatt gctagagaac
     4621 atttccatat ctgcggtgct actggggtgg ctgagtacgc tatgatggcg cgtgatgtca
     4681 tgattgccta tgcaactaaa gcccatgttc acatccagca tttgtctaag gaagaaagtg
     4741 ttaaagtagt ggagtttgct caggggttag gtgcagaagt cacagcagaa gtagcgccac
     4801 agcatttctc taagacagaa gcacttcttt taacacaagg tagcaatgct aagatgaatc
     4861 caccgcttcg tttggaatca gaccgtcgtg ccgttatcga aggtctcaaa tcaggtgtca
     4921 tcacagttat tgcgactgac cacgcgcctc atcatgtaga tgaaaaaaat gttgaggata
     4981 ttaccaaagc gccatctggt atgactggct tagaaacatc cctgtctctc ggcttgacct
     5041 atttagtaga agctggtgag ttgagcttga tggaattact tgaaaaaatg acatacaacc
     5101 cagccaagct ttacaacttt gaagcaggtt acttggctga gaatggtcca gcagatatca
     5161 ctatttttga tgccaaggct gaccgccttg tggactccca ttttgcttcc aaagcagcta
     5221 attcaccatt catcggtgaa accttaaaag ggcaggttaa atataccatc tgtaagggac
     5281 aaatcgtcta tcaagcttga taggagttag aatatgaatt attttcgaaa acgtagggag
     5341 agacaagcga aatctaatag tggaatttac tgtcccgcag ctaatcgttc caaaattcaa
     5401 tatgaaacta aagaaaaagc tgaccatgcg gttcaatatg ataacggagg tttagttaga
     5461 agctattatt gtcgtacatg tgcgtgttgg catactactt caaaatctaa taaacctccg
     5521 ctaagtttga aaaactatgg tctgaaactc tttggtttag ataaatcaga atagaaagag
     5581 aagtatgtat aagacaaagt gtttacgaga gaagttagta ttatttttaa aaattttctt
     5641 cccaatcctg atctaccaat ttgccaatta ttctgcctct tttgttgata ctgcaatgac
     5701 aggtcaatac aacactatgg acttggctgg tgtatctatg gcaaccagta tctggaatcc
     5761 tttctttaca tttctaacag ggattgtgtc agccttggtg cctatcattg gtcaccatct
     5821 tggtcgaggc aaaaaggaag aagttgcgtc tgatttttac caatttattt atttggcctt
     5881 gggcctatct gtggtcttgc tggggatggt acttttcttg gcaccaacaa tcttgaatca
     5941 tattgggtta gaagcagcag tagcggcagt agcggttcgc tatctttggt ttttatctat
     6001 cgggattatc cccttgttgc tctttagcgt cattcgttcc ttgctggatt cgctgggctt
     6061 gaccaaactg tccatgtacc tcatgctttt gttactccct ctcaatagcg gatttaacta
     6121 tctcttgatt tacggtgcct ttggtgttcc agaactggga ggggctggtg ctggtttagg
     6181 aacatccttg gcctactggg tcttgcttgg gatttctgtt ctggttttat ttaaacagga
     6241 gaagctcaaa gccttacacc ttgagaaacg aattccactt aatatggata aaattaagga
     6301 aggagttcgt ttaggtctgc ctattggggg aactgtcttc gcggaagtgg ctatcttttc
     6361 agtggttggc ttgattatgg ctaagttttc gtccttgatt atagctagtc accagtcagc
     6421 tatgaacttt tcaagtctta tgtacgcctt tcctatgagt atctcatcgg ctatggctat
     6481 tgtcgtttcc tatgaagtgg gagccaagcg atttgatgat gcgaaaacct atattggtct
     6541 aggaagatgg actgccctca tttttgcggc cttcacctta accttccttt acatttttag
     6601 gggaaatgtg gccagtcttt atggtaacga cccaaaattt atcgatttga cagcgcgttt
     6661 tttaacttat agtcttttct tccagttagc agataccttt gcggcgccgc ttcagggaat
     6721 tttgcggggg tataaggata cagttattcc tttttacctt ggtttgcttg gttattgggg
     6781 cgtagcaatc cctgtggcta cgctatttga ttccctaaca gattttggag cttattctta
     6841 ctggatcggc ttgattatta gtttgattgt gagtggggcg ctctaccgtt ggcgtttaac
     6901 tgtgattatg aagagatttg aatctttagc aaaatccaaa cgctaaaaag tttgtgaaaa
     6961 aatattcttg ataagaaaat cccttgtttt cacggggttt tcttttttat tttgtgaaat
     7021 ttcttttaga aaaactttga cagtatttgc caaaaacggt aaaatagtaa ggtaagaaga
     7081 aagttagaaa ggcaagaaga tgtcaacttt agataaaaat cttttgctag aaatgttccg
     7141 taagatggaa gaaatccgtc gcatggactt aaaaattgca caattagtaa agaaagggaa
     7201 agtgccagga atgacgcact tttctgttgg tgaagaggca gctaacgtgg gggctatgtt
     7261 agctctcaat ccagatgatc tgattacctc aaaccaccgt ggacacgggc aagctattgc
     7321 taaagggatc gacctcaacg gaatgatggc tgaaatcctt ggtaaataca ctggaacctg
     7381 taaagggaaa ggtggatcta tgcatatcgc tgaccttgat gctgggaacc ttggtgccaa
     7441 tggtatcgta ggtggtggta tggggatcgc tgtcggtgca gccctcagtc agcaaatgca
     7501 aaataccggt aaaatcgttg tctgcttctt tggagatggt gcgaccaatg aaggtgtttt
     7561 ccacgaagca gtgaacatgg cttctatctg gaacctgcca gtcattttct attgcattaa
     7621 caacggttac ggtatctctg cggatatcaa gaaaatgacc aatatagaac atatccatca
     7681 acgtagcgcc gcttatggaa ttcctggaat gttcatcgaa gacggtaaca atgtcatcga
     7741 tgtctatgaa ggatttcaga aagctgtaga ccatgttcgc agtggcaatg gtccagtctt
     7801 gattgaaagt gtaacttatc gctggctcgg tcactcatca tctgaccctg gtaaatatcg
     7861 tacgcgtgaa gaagtggaat tgtggaaaca aaaagatcca atcgaaaacc tccgcaatta
     7921 ccttattgaa aataacattg caagtgccga agaattggaa gaaatccaag cgcaagtaaa
     7981 ggaagcagta gaagcttctg ttaaatttgc agaggaaagc ccattcccac cgcttgaatc
     8041 agcctttgaa gatatttacg cagactaagg agaaagagat aaaatggaaa caaaaacaat
     8101 gtccttccgt gacaccatta tccttgctat gtctgaggaa atgcgtcgcg atgaaaatgt
     8161 gttcttgatg ggagaagacg tcggtgtctt cggaggagac ttcggtactt ctgttggaat
     8221 gcttgaagaa tttggtccag aacgcgtccg tgactgtccg atttctgaag ctgccatctc
     8281 tggagcagca gcaggagcag ccatgacagg acttcgtcca atcgtcgata tgaccttcat
     8341 ggacttctct gttattgcca tggacaatat cgtcaaccaa gctgctaaaa cacgttacat
     8401 gtttggtggt aaaggtcagg ttccaatgac tgttcgttgt gcagctggta acggagttgg
     8461 ctctgccgcc cagcactcac aatctctaga gtcttggttt actcacattc caggacttaa
     8521 ggttgtggca cctggaacac ctgcggacat gaaaggcctg cttaagtctt ctatccgtga
     8581 taaaaaccca gttattatac ttgagtacaa gtcagaattt aaccaaaaag gggaagtgcc
     8641 agttgatcca gactacacaa ttccacttgg ggttggtgaa atcaaacgcc aaggaacgga
     8701 tgtaacagtt gttacttatg gaaaaatgct tcgccgtgtg gttcaagctg ctgaagaatt
     8761 agcagaagaa ggaatttcag ttgaaattgt tgacccacgt acccttgttc cgcttgataa
     8821 ggatatcatc ataaactcag tgaagaagac tggtaaggtt gttctggtca acgatgccca
     8881 caaaacaagt ggctatatca gtgaaatttc agctattatt tcagaatcag aagcatttga
     8941 ctatctagat gcaccaatcc gccgttgtgc aggagaagat gtgccgatgc cttatgcaca
     9001 aaacctagaa aatgcaatga ttccaacagt tgaaagtatc aaagatgcaa tccgaaaaac
     9061 ttataacaaa gaatagagtt acataagata agttatgtaa ataaaataaa gctgacgaga
     9121 aactttgtat cttttaggtg cagagctctt cgtcattaat atcttatatt cgagcacggg
     9181 ctaaaagctc ggaaaaaaga taaatctcct tggcttcatc gaagccatcg tcgatttcct
     9241 atttttcagt cgcttttgac gccctttgta tcataaatag aattggagag gtcatggctg
     9301 atgacaagct aagagcgact cctgcagcta gaaagttagc ggatgattta gggataaacc
     9361 tctacgacgt ttctggctca ggtgcaaacg gtcgtgtcca caaagaagac gtggaaactt
     9421 ataaagacac aaacgtggtt cgcatttcgc cacttgcaaa acgaattgcc ctcgaacata
     9481 acattgcttg gcaggaaatc caaggaaccg gtcatcgtgg taaaatcatg aagaaggatg
     9541 ttttggccct gcttcctgaa aatatcgaaa acgatagcat caaatctcct gctcagattg
     9601 aaaaagtgga agaagtccct gataacgtaa caccatatgg tgaaatcgag cgtattccaa
     9661 tgacaccaat gcgtaaggtt attgcccaac gcatggttga atcttacttg actgcgccaa
     9721 ccttcaccct caactatgaa gttgatatga ctgaaatgtt ggctcttcgt aagaaggttc
     9781 ttgagccaat catggaagca actgggaaga agactactgt aacagacctt ctttcgcttg
     9841 cagttgttaa gactcttatg aaacacccat acatcaacgc ttcattgaca gaagatggca
     9901 agactattat cactcacaac tatgtcaatc ttgccatggc agttgggatg gataatggat
     9961 tgatgacacc tgttgtttac aatgctgaga agatgagtct ttcagaacta gttgtagcct
    10021 ttaaggatgt tattggccgt accttggatg gtaaattggc tccaagtgag ttacaaaatt
    10081 caacattcac aatcagtaat ttgggaatgt ttggtgttca gtcctttggc ccgattatta
    10141 accaacccaa ctcagctatc cttggtgtca gttcgacaat cgagaagcca gttgtcgtca
    10201 atggtgaaat tgtgattcgc ccaatcatga gtttaggatt aaccattgac caccgtgtcg
    10261 tagatggtat ggctggtgct aagtttatga aggacttgaa agagttgatt gaaaccccaa
    10321 tctcaatgtt gatttaagag caagagtatt catttttaag atatagataa aggaaggaag
    10381 acatggcctt agaaataatt atgccaaaag ccggcgtgga tatgacagaa ggacaaatcg
    10441 tccaatggaa taaaaaagtc ggagaatttg taaaagaagg agaaatcctt ttggaaatca
    10501 tgactgataa agtcagcatg gaattggaag ccgaagaaga tgggtacttg attgccattc
    10561 tcaaaggaga tggtgaaact gtccctgtaa cggaaattat cggttacctt ggtgaagaaa
    10621 gggaaaacat cccaacagct ggagcagcat cgccagaagc tagccctgta cctgtagcaa
    10681 gtacctcaaa cgatgatggt aagagcgatg atgcttttga tatcgttgtg attggtggag
    10741 gtcctgctgg ttatgttgca gccattaaag ctgcccaact cggcggtaag gttgcccttg
    10801 ttgagaaatc tgaacttggt ggaacctgct taaaccgtgg atgtattcca accaagacct
    10861 accttcataa cgctgaaatt attgaaaata tcggtcatgc tgcaaatcgt ggtatcgtga
    10921 ttgaaaatcc taacttcact gttgatatgg aaaaactttt agaaactaaa tctaaagttg
    10981 ttaatactct tgttggtggt gttgcaggac ttcttcgtag ctacggagtt actgttcata
    11041 aaggaattgg tacaatcact aaagacaaga acgtcttggt aaatggttct gaattgcttg
    11101 aaaccaagaa aatcattctt gctggtggtt caaaagtcag caagatcaac gtccctggta
    11161 tgcaatctcc acttgtcatg actagtgatg acattcttga aatgaacgaa gtgccagaaa
    11221 gccttgttat catcggtggt ggagttgtcg gtatcgaact cggtcaggcc ttcatgacat
    11281 ttggttcaaa agtgactgtt atcgaaatga tggaccgtat agttccagct atggatgcgg
    11341 aagtttctaa gaaccttcgc ttgatccttg agcgtaaagg aatgaccatc ttgactggta
    11401 ctaaactgca agaaatcatt gaggaaaatg gtcaacttcg tatcaaggtt gaaggaaaag
    11461 acgatatcat cgcaagcaaa gctcttcttt caattggtcg tatgccagac cttgaaggta
    11521 ttggagaggt tgagtttgaa ttggatcgtg gttgtatcaa ggtcaacgaa tacatggaaa
    11581 cttcagttcc aggcatttat gcaccaggtg acatcaacgg tactaagatg ttggctcacg
    11641 cagctttccg catgggtgaa gtttccgctg aaaatgccct taaaggaaat catgcagttg
    11701 ccaaattgaa tttgactcct gcagccatct acactctccc tgaagtagca gcagtaggtt
    11761 tgacagaaga acaagcccgt gagaaatacg atgttgccat cggtaagttt aactttgctg
    11821 ctaacggtcg tgctattgca tctgacgcag ctcaaggttt cgtaaaagtt atcgctgata
    11881 agaaatacgg agaaatcatt ggtgtacaca ttattggtcc tgcagccgca gaattaataa
    11941 acgaagcatc aagcatcatc gaaatggaaa tcactgttga ggaaatgctg aagaccatcc
    12001 acggacaccc aacttactct gaagtgatgt atgaagcgtt tgcagatgtt ctaggaatgg
    12061 ccatccattc acctaagaaa aaataaaacg aagatagatt agactggaag gttatttcca
    12121 gtctctatca tataaagtaa agggatatca tgaaatacat tatcaatcat tcaaatgaca
    12181 ctgcttttaa tattgccttg gaagaatacg cctttaaaca ccttttggat gaggatcaaa
    12241 tcttcctact ttggattaac aagccatcta tcattgttgg tcgtcaccag aacactatcg
    12301 aagaaatcaa ccgtgattat gttcgcgaaa atggtattga ggtagttcgc cgtattagtg
    12361 gtggtggagc tgtttaccac gatttaaata acctcaacta cacgatcatc tccaaagaag
    12421 atgaaaataa ggcatttgac ttcaagagct tctcaactcc ggttatcaat accctggctc
    12481 aactcggggt taaagctgag ttcacaggtc gtaatgacct tgagattgat ggtaagaagt
    12541 tctgtggcaa tgcccaagcc tatatcaacg gccgtatcat gcaccacggt tgcttgctct
    12601 ttgacgttga tttgtcagtc ctcgcaaatg cccttaaggt ttcaaaagat aaatttgaat
    12661 caaaaggtgt gaaatccgtc cgtgcccgtg taaccaatat tatcaatgaa ttaccaaaaa
    12721 aaatcacagt cgaaaaattc cgtgatttac tcttggaata catgaaaaaa gagtacccag
    12781 agatgactga atacgtcttt tcagaagaag aattggccga aatcaatcac atcaaggata
    12841 ctaagtttgg aacttgggac tggaactatg gtaaatcacc tgaatttaac gtccgtcgtg
    12901 gaaccaaatt cactagtggt aaggttgaag tctttgctaa cgttactgaa tcaaaaatcc
    12961 aagacatcaa gatttatggt gacttctttg gtattgaaga cgttgctgca gtagaagatg
    13021 tccttcgtgg ggtgaaatac gaacgcgaag atgttcttaa ggcgctaaaa accattgata
    13081 tcacacgcta cttcgctggt attagccgtg aagaaatcgc tgaagcggta gttggataaa
    13141 tcaaaaaaag aagttggcca acttcttttt ttgatattta tttacataat ttaaaatacg
    13201 tgaaatcata aactatctag agcattcttt tgttcatcat taacaatatg ggtatagagg
    13261 tcagtgactt gtgtgctggc atgtcctagt tggtgactga ctaaaacttg tgatttagtc
    13321 gcatcataga gcctagttgc tagcgtatgg cgcagtttat ggggtgttac acgtacttta
    13381 aaatcctctg agtatttagc aaccattttc tcaacgctag aagcatcgat acgattagga
    13441 acacctctgt agagagttaa aaaaagggct gtatctgttt tttccgtttt atagcgttga
    13501 ttccgaatgg ccagataatt ctctaaataa ggtttagcaa aagcagcgac attgactgag
    13561 tcacgtttgc caccttttcg agtaacatca ataaccatca tttttagatt gagatctctt
    13621 agatctagat taacagcttc agataagcga acaccagatg ccaagagaag ggcaataatg
    13681 gctaaatctc gttctttatt tttgttgaat gatgagagag ctcgatttga aagctgttgt
    13741 gggtgctctt gatcgatata agttagaaaa ccttctgttt catcacctag aaagagtttt
    13801 tgcttgatat tttcagctct ggcagcaagg gtttctttct ttttcttggt tgaaactttt
    13861 ttcattacat tacgatagaa ataaggttcc ccctgatcgt tttcaacctc ctcggttaga
    13921 tacttgtaaa gactagaaag tgctgataag gttcgattga tagttgtctg tgaaacacct
    13981 tgttttgttg tattagcatt cagcaaggga cgttcacgta gataaaggat aaaggattcc
    14041 atgtctttct tagacatatt ttccaaaact gataaaggaa tatcggatat tttatcagcg
    14101 tttgaaatac cagactccaa aacccagctg aaaaatcggt catattcctt aaggtattcg
    14161 tacagggttg taaaactgta gggcacagcc agcttagatt ggtagtattc cagaacatac
    14221 cagggcatga gttgttttag tttgtcgatt cgttccagta aaatctcacg tttcatgtat
    14281 tttctccata agtaagtata ctagtagtat agcatagact tgtatatttt tcaagaaaat
    14341 tatagttttt cagaaagatg ttataggagt ttttcaaagt gataaaaaga cttagaccta
    14401 caaacaaaac agaataagga aaataataaa aatctcaccc actaaacttc tgttaatact
    14461 acataactga agagctatat aaggagctat gagatacaaa acacattgta taatagattg
    14521 aaactagaat agtacatctg gacttctaaa acattgctag aaattgattt gattgttctg
    14581 attgttttat cctatttcta tttcatttca ctatataatt gctattgttt tctttttcat
    14641 gattttaatc ttactgtaca gatttgatac agtaaactcc tttctagctt tatactttat
    14701 catttctaac ttaattatag tcttattttt aataaagttc aattatttat atgtaaaatt
    14761 cctcatcagt aaaaaataac cgatctcatt tctgagaatc ggttttctaa ataaatcaag
    14821 ctgagtattt acataacata aattatgtaa atactcatac tacaacaaca aatctttgac
    14881 ttttccaatt tcactttttg gaataaccag gtgaatcata tcacccagat acattctggt
    14941 tgagccgtta actgtttggc tcttgccatt atggacttga gttgtgatga ggacgttgtg
    15001 tggtaagttg agttcatgaa cttgtttccc agcaatttta tcagaaactg gtatttcgat
    15061 aagtgtaact tctccttcgc tagatacttc ttctggaagc attttttcca gcatggcttc
    15121 atagactggc gtacctttga gcaaatccat gataatataa gaaacaagag tgacaagacc
    15181 aagtggcatg aggttgcgaa tatctcctac catctcagtt acgaggatca tagcggttaa
    15241 gggagctttt gatatggctc caaaatagcc actcattcct agaatgacaa atatagggaa
    15301 ttgctcttga ctgacaagtc caagattgac acagataaca ccaactaagg aaccaagcaa
    15361 agaaccaaga gctaaaatgg ggaggaaaat tcctcctggc agtccacttc catagctaat
    15421 catactccaa ataaagcgga tcaaaaagta agctaataaa acttggaaac taaaattttg
    15481 ttcagttaaa gaaaggacaa gctgatttcc gccaccaatg atttgaggta agaagattcc
    15541 gactggtatg ataatgataa aagccaagat gggataataa gccctatcca aatggatttt
    15601 ttgaccaatc aagtcataaa ctcttccaac gtttaataca gctttctcat agagaaaacc
    15661 tgaaaatcca aggaaaattc ccatgacgag atatatccaa tactggtcta gggtcatggg
    15721 aggaatgtta tctggcatat ccaatactgg tgtcaaaccg aacatgagta gagacacaaa
    15781 gtttgctacg atgctggctg ctagagttga gacccagaaa aagcgcgaaa aatggtgata
    15841 gacttcttct acaacaaaga gaagtgctgc aataggagca ttaaaggctg cggctaaacc
    15901 tgctgcagct ccactggcaa tcaaggaacg ttcctctact ggactggatt tgagccactt
    15961 ggcaattcct ttaccaccaa ctgctccaag ttgaatgctg ggtccctctc gacccagcat
    16021 gagtccactg gcaatagcaa gaatacctag cacatatttt ttccaaagaa tgccccacca
    16081 gttgagggac atgaggcctt tcagttcggc ttcgacttga ggaattcctg agcctttaat
    16141 atctttttct gaccgtgtta gtttggcact gagccaacag atgagtatat aaaacaaaac
    16201 cagtacaaaa agattgcgca ctaagtaccc ttgatcttga taaactcctt gtatcaggtg
    16261 gaagcccttt tcaattaaga aacgaaagga tccgacgata attccaacga tgaggccgac
    16321 aatgattcct cgaccaactt gggatagtat agtgctggat gcaaaggcga attctttctt
    16381 agaacggact atttccgact gttcctccat ggtatttcct ttcaattaac tttctttttt
    16441 acctaaaacc agtgatttaa cgggttcaaa gctggttcgg tgaattgggg taactcctag
    16501 ttttgtgagg ccttccagat gtttagctgt gccatatcct gcattagtag cgaaatcata
    16561 gccagggaac tgctgatcgt attccttcat caattcatca cgtgttacct tggctactat
    16621 agatgctgct gcgatagaga gggagttggc atctcctttg ataatggagg tttgtgaaat
    16681 gggcaagtcc agtttcatgg catcaatcaa aaggtgctct ggttgagggc tgagctggga
    16741 gattgcttct tgcatggcta gtttggttgc ttcatagata ttgacttggt cgatgacctg
    16801 attatctatg ataccaattc caatcgacaa ggcttggtct tgaacgactt ggaaaatctc
    16861 cagatgtttc tttttaggaa ttttcttgct gtcgttgaga cctttaatct tacaattttt
    16921 aggtaaaata acggccgcag cgactacagg accagcaaga ggaccacgac caacctcatc
    16981 aatacctgca attaaggtca atccttgctt ataaagttct ttttcataag aaagcatgga
    17041 ttccaagcgc aaattttcat ctaattcagc ttgaatggct cttttatgct tgctgatttc
    17101 cttttgaact cctgagcgat tatccttttc aagatctaaa aaaatagggc tttctaactc
    17161 cttgactgtc acaaggagtt ctttgatttc tttaatcgtt gccatcgagg tcttccaatg
    17221 tatctaaggt atagttaccg agtttgccat cacggacttc cttcacgaag agactgtaaa
    17281 aacggtcata gtcatcacgg aaaccgaggg cgcgggtcat atccataata atcacaggcg
    17341 cttcttcttc aattttcatt tgtttgaagc gttcagccag cttttctgga taatgttctt
    17401 tgaaataatt gataccaaaa atggtaacct catccatagg aagcaactga tccttgatag
    17461 ctccagtcaa tgccaactta agtgcaacag tttcatcctc aaacttaggc cagagaatcc
    17521 ccggtgtatc caagatttcc agatctttat tggttttaag ccattgttga ccttttgtga
    17581 cccctggctt gtttccaaca acagcaatct ttttaccagc caaacggttc atcagagttg
    17641 atttaccagc gtttggaatc ccaataatca tggtacgcaa ggtttcaatc tgaatcccac
    17701 gttctttctg gcgagcaatc ttatcagcca tgagcttctt ggccgcatct gttacaactt
    17761 ttacagtcac ttgctctttg gagttgatag ctagcgtctg gattccttgt gattcaaaat
    17821 actgacgcca ttccttggtc attgctggat cagccaagtc ggccttgttt aaaatcaaga
    17881 gttttggttt atcaccaaca atcttggtta acataggatt ttgactagat agaggtaagc
    17941 gtgcatcaac taaaatcgtc acaaaatcaa caaattttaa attctcctgc acctgtcgac
    18001 gagctttaga catgtgacca ggaaaccatt gaatagtagc cattgagttt tttcctttca
    18061 tagcaaggat ttagatcccc tattttattt tactattgtt tgaacataga gagttttcaa
    18121 atgaatgatg tccaaacagt tcttgtttta tgacatagaa atctacttta tattatatca
    18181 tgccttcttt attttgtccg atgatagcct tttaccctat atagaaaaaa gccatcaaat
    18241 gatgactttt cagagggagc aaaaaactgt tcccgtcata ttatgaacca aaagaactaa
    18301 actaccaaca gctaataggc ctaaagctgt aagtgcagaa gttgatgaac ctgttgcagg
    18361 aagagtttcc tgctgataaa taagagcata acttacttca ttatcagtag gaattcctgc
    18421 ttgaatcttg gtttgttctt catcaccttt ttaaacttta tttcctaata gtaaacacta
    18481 ttttttattc tcaaaaattg aacttctaaa atcatttgtt tggtcaagat aggctttaaa
    18541 gattgctctc ttcaacttat gaacggcact gtcgatttct ggatcataat tactccaacg
    18601 tggaccttca gcatccttga gaacagcttg gtccatgagg gcttgcaatt catccacatt
    18661 attgatagtc tttgtgccat agcttggcca tggttttgtc ggatctttaa aggtcacctg
    18721 cttcaagttt ccaaactgat ccactcgttc ttggtacata gctgtcttaa attctgccca
    18781 agttttatac ttaccgtcaa ataccttttt caaaacaagc tcatcggtca ccaagccccg
    18841 ttctttacca taaagattga tagtttgccc ttgttgttta gcatcttctt cgtattggtt
    18901 tgagatataa ggtaccattc catccttaaa gcctttggca gccaaaagtt cgtacgcaat
    18961 cctacgtccc ataaggtccc ctggtgtgcc tttctcactg ctgagagctg aatagattgg
    19021 ggcaaagagt ttgatggtat agtagccatt tcgttcatag tcgccagact ggtactcacg
    19081 agctgataag atgttgtggt caatcaaact ttcaaaacta ttcaggtttc gggcctctgc
    19141 ttctgtcaat tctttgacta cgttagtggc ataaacctca tttccatctg ctggatcttt
    19201 cacatacttg ttctcaattt ttctgagagc cgccattttc tggtaaacac ttagtttatt
    19261 aacgattgac tgcccttcga gatattccat catgtagata aggtcaaaca tgttatggac
    19321 atagttctga agatctgctg cgttttggaa tctctctgtc ggatccaaga cttgaagacg
    19381 ggatccctct gtactatctg attttgagtg tttcaagata gaattaatgg taatggttgc
    19441 gtcacttggt tggtcagggg cttgcaataa gccttttgcg aagaactctg gtcccaagcc
    19501 atttcttcga ccatagccac caaggtaaat atcctgatcc gaatcatggg tcatttcatg
    19561 ggtataagta atggctccat ccttgtccag catgcggtaa gacatatagt agacgccgtc
    19621 ccctgtagcg taggctccat gctggttgtg tactacctta ttgccaactg gtccaaagaa
    19681 gttcttcata gctggattgg agctatcaaa cttagcctcc gctgtagctt ttcctgatgt
    19741 tgtgtcatcc ccaaacttat aggcatcata aaggagaatc gtacgataga gtttttcgcg
    19801 tgactgttca tctaaaatac gataccaata atcgtagtga tctcgctgac gtttggctgt
    19861 ttcacgcgca ttttcttcaa caaaatcatt gagagccttg cccgctttat ggtcactact
    19921 gcggtagcga tcataagctc caaatcctag actagacatg gtcgagatga caaatacgga
    19981 tctctctggc aaggtcagga gaggcaagac catattgcgg tatttccatg tggcactcgt
    20041 gatacgatca taaacaccga tagaatactt ggtgccagct aacccttgct tcgttttcac
    20101 ctcttcgata gtggattttt cttcgacaat gtaagcctta gtctctgatt taaaccagtc
    20161 attattgctt gtatttggta aaaagacttt tcggtaatgt tccagcgtgc taaacaaatc
    20221 tgtcgttcca tgttgactgg caagactgat accataagta tcgacattat tcttagctag
    20281 aagattgtta aagccagatt tacctaactc aatcagagta tctagtgggg aagtatttcc
    20341 tttaccaaag aagtctggat gatacataac taggtctttg acattcactt gaccatagtt
    20401 aaagttatac caacgttcta aataagttaa accgagtagc aaggcttcct tattattctt
    20461 gattttatct acaagatagc ctctggtgac tgagttatcg ctagctagac cggcatctgc
    20521 tgacaaaagt tttttcaagc tgtctcccag attttgtttt gttttggaga actggtcttc
    20581 taaatagagc tcggttaact taacttctgg agaaatacca agtgtctttc tgatagcatc
    20641 tgactggtaa tcaagcttct gcaactcagg caagacttcc ttaacaatag agtctcgatc
    20701 atataagaat tggttaggag tgtagagaag tcccgtatta cctagattat attctgctag
    20761 attggcaaaa tcagtctggt acttgagatc aaatttctca gatgaatggt cattatagtg
    20821 aagtaagagt ttgtttgcag tctgtttgtt ggaaacaata tctgtgatta cttggtcatc
    20881 cttcatcata actgcagata agagttcttt ttggtataag agactgttct ccttaaccag
    20941 gtttccgtat ttgacgatgg ttgacttatt ataaaaaggt aacaattttt caatgttctt
    21001 ataaaccaaa gcacggttag cttgataatg ggctatctta gaaaaatcac tgtctttctt
    21061 gccacttgtt gagaggggtt ccaccgttgg tagagtgaga ggatttattt ctgctttttt
    21121 gcttacaatt tgagaagcat ctagcattgt tcctctttct tcaaaggatt ccttgctgac
    21181 gacctcatcc ttctctaaag tgacattgaa aactctatta gccttactac taaatgtatt
    21241 agctaccttc attcctgtat agtggtatcc agtgatggca tttccgtttg ttacattgac
    21301 atcgctaaga acattggtca attcaccagc gtgtttttcc tcaccagaag ttcgatccca
    21361 caaatatcct gccacaccag cgactttacc aaagtgcttg acattattaa tatcaccttc
    21421 cgcataactg ttctgtatat gcgcatcttg gtctactaga cctgcaagac caccgacagt
    21481 ctgatctgac ctattggtgt ttgacgaaat ggttactgtc gctttggatt tagcaataga
    21541 cgcatttttt cctgttaaat gaccgaccag accgccaata ttgtaggcat cagtcgtttc
    21601 ataggtattg acaattcttc ccttgaaact gctctctgcg atgcttgatt ggtctgcctt
    21661 agccaacaaa ccaccgacac cacgttctcc agccagaaca ccatcaacat gaacttgttt
    21721 aatctttgtg ccattcgtag cttcatttgc tagtgaacca atatcatttt tccctgaaat
    21781 agcaacattt tttagactca gtttttctac tgtagcacca ctcaagtttt caaacagagg
    21841 ttttttcaaa ttatagatag cataattctt gccatctttt tcaccgatta aacgaccagt
    21901 aaaggtgtcc ttgatatagg atctttcatc aggaccaagc tccacttcgt tagcattcag
    21961 gctggccgct aaatgatagg ttccagaggg attttggttt atagctttga ccaggttgct
    22021 aaaggaagta aagtttgtat tttcctcttt agccttctta tcgaggtaga aggtgaaatt
    22081 gtcgacatat ttattttcta gttcttgctg gagtttctca gctttggctg tgattttata
    22141 aactgactgc ccatttcttt tctcttcagt tattgaagct actggtatat agacatcttt
    22201 aaatgctgaa gattttacct tgacaaagta agtatccgta ttctctggaa ttccatccaa
    22261 tgaaacatgt tgtctgtagg ttccattcgt ctgactatac aactcaatat cagaaacatt
    22321 tcttaattcg agttttttct ctggttctgt ttgtccattc tctgatgttg tattacctga
    22381 attttctggt tttgttgcag ttccgttttt ttctggttga tttgattctt caactggtgg
    22441 ttttgaatca ctaggtttat tggatacttc tccagtattt tcgttagcta ttttcccaga
    22501 gtttgtttgt gtttcttctg caggttgaac tggtttttct gtttcttgat ttgaggtacc
    22561 ttctactgtg ccttcatttg gatttactgg aacttcttct acagtttttt ctgaattttc
    22621 atttttagag tcattatgtt ctggtttatt tgattctcca actgaggttg tcgaatcact
    22681 aggattactg gacacttccc cagtattttc gctagatgta tctggtgata ctttctctga
    22741 attcgttgtt gattcttctg caggttgaac tggattttct gcttcttgaa ttgaggttcc
    22801 ttctgtagta ccttcatttg gatttactgg tgtttcttct gttggtttta ctggaacttc
    22861 ttcagttttt tctggacctt gttctttggt cttctcaacc ggagtttcag gttttacttg
    22921 ctcaatatta cccttatatt ctggcaatgg ggctacctgc tctggttcac ctttatcact
    22981 taccacagta tctggcgact ctggttgaac ctcagtctca cctttgtcgg tcacaactgc
    23041 ttcgggtaat gtcggttgaa cttctggttc gcctttatca cttactacag cttcgggcaa
    23101 ctcaggctga attgcgggtt caacaataga tccagactgt acgtccttat gttctacacc
    23161 agtctcaggt tgttccttta taacttgagt ttttttagta cctttttcga ctattcttgg
    23221 actaggcgca gtcgttgaag ttgaaacaat ttctcgcgaa acttcttcct tgtttacaga
    23281 gaatattctg acgatttcaa ctttcttacc taatttacct tcttgtttta ctcttacagt
    23341 tccttcagct aaatcaggat tttcttgaat ttcttcttga aaatctattt ttgtctctat
    23401 agtttcctca cgatataaga gttcaggttt gttcaattga cctgataaaa cttcatcctg
    23461 tggatttaat gtatttaccc cagtcttttc ttttggagaa atcttctcct ctttcttcgt
    23521 ttctagattc ttatgttcgg ctaattgttc ttgagaatct gaagattgtt tctcttcttt
    23581 tcttggattg attaattcag tagagaaagg tttttcaact acttgaactt ctgtcggctt
    23641 agttgaagaa acaggtgttt gttcctgaat agcttgtact gttgatggat ggtctacaaa
    23701 atttggtgta acattataat ccaccttttg ttgttttgta ggagtggctg ctgatttttc
    23761 ttgattactt acttcaaaat cagaagtcgt ttttccctct ttgatatatc caatataagt
    23821 gtaacctgaa atctctttag gaagaggtaa tttttctcca gaggtcaatt catagtccgt
    23881 attgtaattt agcaaaagat gattttctaa agcatggact gaaactaaga caccatttcc
    23941 cattcccgca accaatacta aatgtaatac cgttttattc ttaacctttt tcttggaaac
    24001 agcaaaaatt aaaattccca gagcagctaa gctagcacca gcaactaggg cttgcctctc
    24061 attcttgctt ccagtatttg gtaattccgc cagttgattt tgagaattta acttataaac
    24121 aagataataa gtttcatcat cattctccac gtatgtcgga atatcataga caagctgctt
    24181 cttttcttct gatgatagct ctgaatctgc cacatattta tagtgaactc ccgcagtttc
    24241 ttgagcatcc acagatgaac tagctaatac agacataaaa aataaacttg aaatcgttgc
    24301 agatacaagt cctactgata attttctaaa tgaaaaacgc tcttgttttt caccaaaata
    24361 cttttccatt attcctcctt gaaataaaat ttatatatgt tacaaagacc tttattatat
    24421 tagtgtatta tctattatct atagaaaagg cagtatacct taattatact cttaatttac
    24481 aaaaaagtct taaaattgag atgcgctttc atactttgtt ttatattatt tgggggtaca
    24541 ataacaccta ccatgaaatt tacacggtag gtgttactca tatcactaat cgttctaaaa
    24601 atggtttgag gcagttgagg agaattcctt ctatccagct tccttgtgct gatgagcgat
    24661 ggtcttcctg caggcttttt tttagaaaat ctcggacttg ttctggtgcg atttcaaatt
    24721 caaaggcttt cattttatag aaaaagtcga tgagatgatc tgacaggtat tcagttgaaa
    24781 agggtacttc accacttttt cgatattcta ataagagtct agaaaatcga gctttttctt
    24841 caggaagctc acgaaaatag gaattgagga tccaagtctg cttctgtttt ctttcaattg
    24901 gatcctgact ggcaattcgt tggtcttttt ccagctcttt ttggtattgt ttggccttga
    24961 tagctcgttc tgctctattt ttaccaaaaa gaattttttc ccacttgcgt tcttcttgag
    25021 tcagggtctc tgtaaagcca aagtaatctt gataagcacg ctctgcgggt cccatggcta
    25081 gaaccagatt gtctgcatat tgcttggcga ttttatccct cttcttgcgt tctttctctg
    25141 cctggatacg gagttcttgt tcgtagtcaa ttttctcctt gcctagcttg acaaggtaga
    25201 gttggtcatc cgatttccca agtaaaaagg gtttgataca cttttcaagg acttcttcca
    25261 tccgagcctt tttctttggt tccgccttgg tccaacttcc tccctgaaag acttctagga
    25321 aaagctggta gtctctctca ggcgcaaatt gattgccacg attgggtttg aaaacacctt
    25381 tttcccagag ccattttaga agtcgctcgt caaagtcact tttattgacc ttgatttttt
    25441 cctttttctg agcttttctg gttagatttt caacctttct gagcagtttt tcttcctctt
    25501 ccaattgctg gtcaaggggc aatcgatgaa aatgacgaac acagtcgcta ccaattggaa
    25561 agaggcgttg gcctgtgaca ccgttaaaga gttcataagc gtatttgatg gcatttcgac
    25621 agacacaatt gctacggcca ataccgttaa aaatcaagga aacttcattc cattccttgg
    25681 tagcttgttc ccaagtatcc gctttcgaag cctgtaaaac tgcatcgtgc agggattttc
    25741 taactggaag tgtcatgagg tctcctttct aatactcaat aaaaatcaaa gagcaaacta
    25801 gaaagctagc cgcagtcagc tcaaaacact gttttgaggt tgtagataga actgacgaag
    25861 tcagtaacca tatatacagc aaggcgaagc tgacgtggtt tgaagagatt ttcgaagagt
    25921 ataagttata cttttacaac ttgaacctcg tctttaccga gtaaaatcaa gtatttttca
    25981 atattttcaa tcgaataggc tcgtgataaa gcctcttcgt atagagctaa ctgaccacga
    26041 tagcggtcta cgagttgact tggttcatca tagcggtctg tcttgtagtc gaacagaaca
    26101 attttgtttt cgtaaagcag atagccatca aggataccac ggacaacaaa gtcttcctga
    26161 ctcttttggt ctcgtttgag catggagaaa ggttgctcgc gatagagatg gtcggtatta
    26221 gcaagaattt cctgaccgag tgctgtgtca aagaaagcaa gaattttatc aagattgatc
    26281 ttgtctctga cagcttggct agtttgaact tgtttgagtg tttctgttaa gctagcaagg
    26341 gttagttgct ggctgaggtc aattatctgc atgagttcgt gagtagcact accaatctca
    26401 gctccagtta ccttttcttt ggttgaaaaa tctggcaaat cgaagctgat tttcttgcct
    26461 actgactgac cttgaccagc aatctcgaca ccttccatat ccataactgg ttcgtagaat
    26521 ttcttgattt gacttggggt ttgaacacta ggaagttcaa tagctgcgcg gtgaagagta
    26581 ttataaactt ccacctcctt cagcatttcc agagcttctt tgatggtatc tgactgacga
    26641 ttgtctgctt gggagctatc ttggagagga ctcttggttt ccaactctcc gatagcttct
    26701 ctggtcaact gatcttcgtc aataaaacga taactaaagt tgagcttgtc cttagtaaac
    26761 actttactga tagcccaaag ccaatcttgg aaattccgtg cttgcagtct agtattgcta
    26821 tttagtttcc catttttggc tgctgggtat tccttggatt ccagcttttc acgagaaccc
    26881 ttgccgacaa gatagagctt tttctcagcc cgcgtcatag caacatacag caaacgcatc
    26941 tgctcagaat agcttgctag ctgtaattcc tcttcgttct gcctataggt cagactagga
    27001 atggagagtt tgatggtttt aggatagtgg tcttctactg cccctgtctc catcttggca
    27061 atatatttga caccaagacc attctgacga ctgagaatga cttctgacat agagtcttgc
    27121 ttgttgaaat cttgatccat attgaggata aagacgtaag gaaactccag ccctttactc
    27181 ttgtggatgg tcatgagctc tactgcatct tttggcggtg cgacggccac gcttgccaaa
    27241 tcgtgctggg cttctaagac ttggtcaatc atacgaataa aacgcgacaa acctttgaaa
    27301 ttgctctttt caaattgatc agcacgcagt gctagggcat agagattggc ctgcctagca
    27361 ggaccattcg gcaaagcccc aacatagtca taataaaaac ggtcgttgta aatcttccaa
    27421 atcaagtcat agagagagtg ggttttggca tacaagcgcc aagaagctag gatatccatg
    27481 aattgcttta gtttttcaac tagagctgtg tgaatcaagc ctttttgact acttgccatt
    27541 ttttgtgcat tgaccagttt ctcatagaga ttttcgtgga ctttatcctc tgctttctga
    27601 agggacaaac gtgctagctc atcctcatca aaaccaaaca ttggagactt cataagggca
    27661 accaaggcgt agtcttgcag gggattgtga atgacacgaa gagtgtctag catgacttgc
    27721 acttctaggg attggagata attgttttgc tctccgtcag ttttgacagg aatcccgtac
    27781 tcagacaggg cgaggagaat ctggtcatta cgactgcggc tggaggtcag aagggcaatt
    27841 tccttaaagg caacaccttt ttcttgatga agtttcagaa tctccttgat aactaagcgc
    27901 atttcgcctg ttagtttcgt ttctgtttga ctctcttctt cctcacctgt atcgtccttg
    27961 tcgtagagga gaaattctgc cttgttgtct ggattgggag tcagtttggt attggcaaaa
    28021 acaagctggt gcttgttatc atagttgatt tcaccgacct cttggtccat gagacgttca
    28081 aagacatcat tggttgctga cagcacttct gaactactac ggaaattttc cttgaggaga
    28141 atgagcttgc cttcttgggg attttgcgca tagcgttgga atttctcatt gaaaatctgc
    28201 gggtctgcct gacggaaacg atagatggat tgcttgatat ctcccaccat aaagcgattg
    28261 tggccattag acaacaattc cagcatccgt tcttgaatat ggttggtatc ctgatactca
    28321 tcgaccatga cttcatggaa gcgctcctga taagactcac gaacttgtgg gaaattctct
    28381 aaaatctcaa tggtgtaatg gctgatatca gcgaattcaa aggcattttc ctggcgtttg
    28441 cgctgacgat aagcctccac aaaatgactc ataaaggttt ggaaggtttt agctagtttc
    28501 catgtgtctt gatgataacg ttcttgatag tcgagaatgg ttatctggtc tgataattgt
    28561 cctagtttag caaactgggt ctttctctct tcgttgtagg catcagccag gggcttcaaa
    28621 tcagccttac gactggcatt agtcagagct cgaccgtttt tctccttaga gatggcgaca
    28681 acacgcgcaa gcactgcctg ataagcctga ctatcggact cctgatttag ggagcaaatt
    28741 tcatccagaa ttaactgaac attttctaaa taggcagcct ttgcaaactc cttggcatcg
    28801 ttatccagat ggtaacggaa aaagctttcc aaatcccaaa gggcttgttt gatttgctca
    28861 gtcagttttt ctttttcact ggtaaaatca gctttctcaa atcctttgag gaaagattca
    28921 ctcagccact tttgaggatt actggtggat tggaggaagt catagatttt atagacctgc
    28981 tggcgcagac cccgttcgtc cttgccacgc ccagcaaagt ttttcagcaa atgactaaag
    29041 gtctctttct gtttaccttg gtaatgcgct tcaaagacct catgaaagac ttcgttttcg
    29101 agaagaagtt gctcgctttg gttttgtaaa atacggaaat taggtgcaat atcaagcaga
    29161 taaccatgtt tgccaaggaa tttttgtgtg aaagagtcca tggttccgat ggcagcgttt
    29221 ggtaggtctg ccaactggcg tcccaagtgt tgtttgaggt cgacatcatc tgtttcttgg
    29281 attttcttgc tgattttttt ctctaaacgt tctttaagtt cagttgcagc cttgacggta
    29341 aaggttgaga taaagagttg agaaatttcg acaccacgcg ccaattggtc cagaatgcgc
    29401 tctgccatga caaaggtctt tccagaacca gccgatgctg agaccaggat attctgacca
    29461 gaagtgtaga tagcttcgat ttgctcggca gttttctttt gttccttgct cgaatttgct
    29521 tctgcttctt gcagtttttg aatctcctcc tcacttaaaa agggaataag cttcatcgat
    29581 tcaactcctc tcttattttt tcaagccaag cttgcttgag tttttctccg accagacgct
    29641 tgccatcagc taggtccaac ttttctagga aacgggcttg gcccagatgg taattggctt
    29701 caaagcctgt aatagcctga tgttgctgga cgtatggggc aatgcttctg ccattttcag
    29761 tataaggatt gatggcgaac cggcctgcta aaatcttctc agcagctttc ttgtaaagat
    29821 aggcattgta gtccagtagg agctgaaatt cctcatctgt cagttgattc gccttgtttt
    29881 tgttataaaa ttcgcctaaa taactgcttt ctttttccaa gaagagccct tggtatttca
    29941 tagatttgct ggcttctacc actgctcctg ccagactttt taccgccatc agagattgga
    30001 caggttcagc catttccaag tacatggcgc cgaaaaagtt ctgctcccct tctcttttta
    30061 gggcagcaag ataggttggt aactgagaat tgagcccatt aaagaaatga ggaaactgga
    30121 actgagtcag actggatttg tagtctacta ctcctatcgc tccattagct ttcaaacggt
    30181 caatccggtc caccttgcct cgtacaaaga cactgcgtcc attgtctaat tgaataaagg
    30241 cttggtcttt tccaccaaaa tttgcttctt ctttgattgt ttcgatggct ggattgtgtc
    30301 ggagaatatg tccagttgtc cgtgcaacat caagcaaaac ttccttggta aactgggctt
    30361 ccaaactttc ttgataaata gcttcaaatt cgcgttcttg actggtttct tgaatagctt
    30421 gttctagacg ttggtcaaag gaatcttcat taggcaactg caaggcgcgt tcaaagatac
    30481 gatgcaagaa attcccgtga ctacgggcat caggacgcaa acgtaattcc tcctgcaagc
    30541 ctaaaacgta gcgtaggaaa taactgtatt cattgcgata aaactctgtc aaccccgacg
    30601 tagacaggta aaactcctgt ttggcaggat agagagcttg caaggtgtcc ttggctaagg
    30661 tcttgctgct tggactgatt gggatggctg gattttccag accttgctga tctagttttt
    30721 tacctatgac acgcgacaga accttgacaa aagtcaaatc ttgctcagta tcgctcatct
    30781 caccctgctg gtgataggca accagactag acaaaagact gtgataggac cccatatcct
    30841 ccttagacag tcctttgtga ttcatcctct tctctctccg cctaaatcca aaatggatca
    30901 actcttgaag ataggcagat tccttacttt cactttcgtt aaaaaggctt ggagccgaca
    30961 agaacaactg cttacgagca gaattgacca aggaaagcat agtgtagcga tttttcttga
    31021 gattttcact gctggcaatc agtaattgaa cgccttcttc ggtcgtttgg tttaggtttt
    31081 gcctttcttc atctgtcaga agactggtgt tttgagaaat ttttggtaaa ttgtcctgag
    31141 ttagtccaat agcatagaca aagtcagcag tcaatggtgc aatcaaatcg taactctgca
    31201 ccagaacagt gtccactgtt gctggaatgg tacggtattg ggacaaactc attccagaat
    31261 ggagcaaggc taggaagtct tccagactaa cctgtgaacc agcaaaaaca gtcgcaaatt
    31321 gttctaaaac atggcagaaa gccttccaaa cttcggcttg tctttcctgt tctacagctt
    31381 ccaaagtggt tgtcaaatct tgtaactgct tggtcacagc tccttctttt agaaagacac
    31441 tccatttttg taggaggttt tcagcctttt gttttcggct ggcaaagagg gtttcaagag
    31501 gtgctaaaat tctcaggcgg aggacattca aacgctcaag attaaatttt ccatggtggg
    31561 atttggtgaa agtttgctga aaggctggca agccattgat accaagatag cggatatatt
    31621 gctcaaaagc atcaatatca gactgactga ggtcagtata caaatcagtt ctaagaagat
    31681 taatcaaatc ctcctgacga aaacggtaac gttttaaagc taaaatagac tcgacaaact
    31741 gagtcaaggg atgatgagcc atggcttcgc ttctaccaag ataaaaagga atctgatact
    31801 ggtcaaaaat ggttttgaga gataactggt aagaagctac atcccccaag agaatacgaa
    31861 aatgcttgta gctcaggtct gagttctcat gtaatttctg acgaatgcta cgggctacta
    31921 gctccaactc ctccttttgc gtcaaacaag accagatttg taaattttca cggtctttct
    31981 catcgacatc caaagcgagt tctgaaaagt cataagaaga ctccaacaaa cgagaggcct
    32041 tgtcaaaact atccatcttc tcatgagttt gagaacagtc ctgagcaggc gtttggtatt
    32101 tagaagccag atgatggaga aattttacgc tggcttggta gagattgccc tcgctaaaag
    32161 gactggtata ggctttctta ctagcataag ccccgataac aatctcaaca cctttgccgt
    32221 gaagtaagtc cacaacccgc tcttcctcag cagaaaaacg agtaaagccg tcaatgacca
    32281 aggtgatttg attaaaatca ctacttacct tgtcattctc aatagcctca atcaaatggg
    32341 acaactgact tccctgggct aactgacctt gattaagata ggctgttact ttctcaaaaa
    32401 tcaagagtaa atccgccctc ttatcctcat ctgttaaatt ctccaagtcc aaaaaactca
    32461 tctgagattt ggtcatctca tggtaaagct caattaactg ctggatcaat tgaggatcct
    32521 gcttaatagc gccataaaca cgcaagtcct tgggatcgag ttcggcaagg catttgtaaa
    32581 aggccaaccc aagaccgata tcatcaagag tagttttaac tggtaaatca ttcaagacca
    32641 gataacgagc catttgagca aagcgcgtga cggtaatcga aaaagaagcc tgctgggaca
    32701 agcattccag cacggcgcgt tccttttcaa aagaaagaga gttgggggca atgtagaaga
    32761 cccgcttgcc agctgcaact agctcttctg cctctcttgt tagaatttct gtcaaagaag
    32821 ttcgaatatc agtataaagt aatttcatct cagcctcgtt ggaatttttc atcaccctat
    32881 attataccat gattagcctc gtaaatctgt taaaatattt aggccatcct ttcttttctt
    32941 catcatctgc taaatcttaa atacttagct ttacttgtat tagatagaat aagtctggct
    33001 actgaaaatc acataataaa aaagcctcgg taacaaggct ttgagtttta tgattgtttc
    33061 ttaggtacgg aatacacttc aatgtgttgt cccagtatct taatgtcgac tggtagattg
    33121 tctgatttat cgccatcaac atcggactct aattcgatat cagaagaagt tttaatatta
    33181 cgtgccttta tatattcaat attcttgata gaatgattga actatagtaa attgaaacta
    33241 taatagtaca ccgtggatgc taaaatattt ctagaaatta atttgatttc cctaatcaag
    33301 ctgttcgtat cttatttcaa tcta
//
LOCUS       DAVBCN010000015        32649 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVBCN010000015 DAVBCN010000000
VERSION     DAVBCN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32649
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.015"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(283..972)
                     /gene="rplA"
                     /locus_tag="VQ497_000654"
     CDS             complement(283..972)
                     /gene="rplA"
                     /locus_tag="VQ497_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET2517000.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMGLVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(1179..1604)
                     /gene="rplK"
                     /locus_tag="VQ497_000655"
     CDS             complement(1179..1604)
                     /gene="rplK"
                     /locus_tag="VQ497_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET2517001.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAVRMIEGTARSMGFTVID"
     gene            complement(1727..2443)
                     /locus_tag="VQ497_000656"
     CDS             complement(1727..2443)
                     /locus_tag="VQ497_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET2517002.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKAKNEDGAAKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     VIGTNGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYFGFEGGDYVD"
     gene            complement(2446..2916)
                     /locus_tag="VQ497_000657"
     CDS             complement(2446..2916)
                     /locus_tag="VQ497_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET2517003.1"
                     /translation="MCLICQRIELIKKGENPYFVKELETGYLVIGDHQYFAGYSLFLA
                     KEHVSELHHLEKKTRIRFLEEMSVVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRR
                     RGDMNGYGLNGRGPVWWIPFEEMTAEICQSKPDEIKRLVERLSSEVDKLLEIKE"
     gene            complement(2918..3955)
                     /locus_tag="VQ497_000658"
     CDS             complement(2918..3955)
                     /locus_tag="VQ497_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HET2517004.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVLGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAMLLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTNEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLKSALVD"
     gene            complement(4053..5378)
                     /gene="brnQ"
                     /locus_tag="VQ497_000659"
     CDS             complement(4053..5378)
                     /gene="brnQ"
                     /locus_tag="VQ497_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HET2517005.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(5549..5641)
                     /locus_tag="VQ497_000660"
     CDS             complement(5549..5641)
                     /locus_tag="VQ497_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2517006.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(5684..5836)
                     /locus_tag="VQ497_000661"
     CDS             complement(5684..5836)
                     /locus_tag="VQ497_000661"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2517007.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            complement(5996..6574)
                     /locus_tag="VQ497_000662"
     CDS             complement(5996..6574)
                     /locus_tag="VQ497_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET2517008.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPCT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(6628..8028)
                     /gene="pepV"
                     /locus_tag="VQ497_000663"
     CDS             complement(6628..8028)
                     /gene="pepV"
                     /locus_tag="VQ497_000663"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HET2517009.1"
                     /translation="MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLTDLQAKLDAFVAEHKLRGELQEEAGQYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(8041..8646)
                     /locus_tag="VQ497_000664"
     CDS             complement(8041..8646)
                     /locus_tag="VQ497_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET2517010.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDLKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            8817..9617
                     /locus_tag="VQ497_000665"
     CDS             8817..9617
                     /locus_tag="VQ497_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2517011.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(9799..10632)
                     /locus_tag="VQ497_000666"
     CDS             complement(9799..10632)
                     /locus_tag="VQ497_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET2517012.1"
                     /translation="MNHKIAILSDIHGNATALEAVIADAKTQGASEYWLMGDIFLPGS
                     GANDLVALLKELPITASVRGNWNDCVLEALDGQYGLEDPQEIQLMRMTQFLMERMDPA
                     TTVWLRSLPLLEKKEIAGLRFSISHNLPNKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELVNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHGYVEDVKNFFDF
                     L"
     gene            complement(10622..12469)
                     /gene="uvrC"
                     /locus_tag="VQ497_000667"
     CDS             complement(10622..12469)
                     /gene="uvrC"
                     /locus_tag="VQ497_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET2517013.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGIHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDDKIIDDLKSKMAVAAQSMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGRPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQRKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            complement(12509..13210)
                     /locus_tag="VQ497_000668"
     CDS             complement(12509..13210)
                     /locus_tag="VQ497_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HET2517014.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKMGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(13289..14521)
                     /gene="murN"
                     /locus_tag="VQ497_000669"
     CDS             complement(13289..14521)
                     /gene="murN"
                     /locus_tag="VQ497_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HET2517015.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSEYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(14526..15746)
                     /gene="murM"
                     /locus_tag="VQ497_000670"
     CDS             complement(14526..15746)
                     /gene="murM"
                     /locus_tag="VQ497_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HET2517016.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(15858..16637)
                     /locus_tag="VQ497_000671"
     CDS             complement(15858..16637)
                     /locus_tag="VQ497_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HET2517017.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(16706..18367)
                     /locus_tag="VQ497_000672"
     CDS             complement(16706..18367)
                     /locus_tag="VQ497_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET2517018.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPEHIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKILSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(18542..20764)
                     /gene="recJ"
                     /locus_tag="VQ497_000673"
     CDS             complement(18542..20764)
                     /gene="recJ"
                     /locus_tag="VQ497_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET2517019.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKTYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            21041..21799
                     /locus_tag="VQ497_000674"
                     /pseudo
     CDS             21041..21799
                     /locus_tag="VQ497_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            21839..22603
                     /locus_tag="VQ497_000675"
     CDS             21839..22603
                     /locus_tag="VQ497_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET2517020.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTELLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            22616..23293
                     /locus_tag="VQ497_000676"
     CDS             22616..23293
                     /locus_tag="VQ497_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2517021.1"
                     /translation="MIDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILAHIFVEFYQNTPLLVQFVIVFYGLPLISDHTIMIPIYWTAVLCMGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQTFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            23303..23962
                     /locus_tag="VQ497_000677"
     CDS             23303..23962
                     /locus_tag="VQ497_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2517022.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNTVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLCILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            24257..25447
                     /locus_tag="VQ497_000678"
     CDS             24257..25447
                     /locus_tag="VQ497_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HET2517023.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTNNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(25768..26649)
                     /locus_tag="VQ497_000679"
     CDS             complement(25768..26649)
                     /locus_tag="VQ497_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HET2517024.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(26790..28118)
                     /gene="vncS"
                     /locus_tag="VQ497_000680"
     CDS             complement(26790..28118)
                     /gene="vncS"
                     /locus_tag="VQ497_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HET2517025.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGATMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(28115..28771)
                     /gene="vncR"
                     /locus_tag="VQ497_000681"
     CDS             complement(28115..28771)
                     /gene="vncR"
                     /locus_tag="VQ497_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HET2517026.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASIAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(28820..28903)
                     /gene="pep27"
                     /locus_tag="VQ497_000682"
     CDS             complement(28820..28903)
                     /gene="pep27"
                     /locus_tag="VQ497_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198524447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HET2517027.1"
                     /translation="MRKEFHNVLSSGQLLADKRPARDYNRK"
     gene            complement(29083..30462)
                     /gene="vex3"
                     /locus_tag="VQ497_000683"
     CDS             complement(29083..30462)
                     /gene="vex3"
                     /locus_tag="VQ497_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HET2517028.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(30514..31161)
                     /gene="vex2"
                     /locus_tag="VQ497_000684"
     CDS             complement(30514..31161)
                     /gene="vex2"
                     /locus_tag="VQ497_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HET2517029.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKDTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(31174..32451)
                     /locus_tag="VQ497_000685"
     CDS             complement(31174..32451)
                     /locus_tag="VQ497_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2517030.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITSYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         9216 a         7486 c         6070 g         9877 t
ORIGIN      
        1 ctcttatttc attttactat aattgatagt ggtcgcccca gccagatacc ttatccgcta
       61 tataggcctt attatcatgt tcctgttcgt agagccacga tttcgctatc ccttcctctg
      121 acgattctta tttcctgcgt cagacttggg aatcgctatt gggttcaccg gtagcaggtg
      181 cccaccgagg acttacacct cagatatacg acatgcccgt cgtacatcaa aaaaagttga
      241 gacatagcgt cttcaacttt tttacattac tttaactact gattaaagtg agtttacgtc
      301 aactttgata ccaacacctt gagtagttgt gatagtcaag tttgttacgt aagttccttt
      361 agctgtagct ggttttgctt tttggattgt ttcgttgaaa gctttaaagt tttcaaccaa
      421 tttttcagct tcaaatgata ctttaccgat gattgcttga acgttacctg cacggtcagc
      481 acggtaagtg attttaccac ctttagactc ttcaactgct ttagcaacat ccattgttac
      541 agtaccagtt ttagggtttg gcatcaagtt acgtggtcca aggacacgtc caagacgtcc
      601 aacaagaccc atcatatcag gtgtagcgat aactacgtcg aagtccaacc aaccgtcgtt
      661 gattttagca acaaggtcat cttcaccaac aaagtctgca ccagcagctt ttgcttcttc
      721 agcttttgca ccacgtgcga aaacaagaac gcgtgaagtt ttaccagtac cgtttggcaa
      781 taccattgct ccacggattt gttgatcagc ttttttaacg tcgatgttca agttgtaagc
      841 aacttctaca gttgcgtcaa attttgcaaa gtttgtttct tttgcaagtg ctacagcttc
      901 ttctacgctg tatgcttttg tgctgtcgat tttctcaaga gcagcacgaa gttgtttgct
      961 ttttttagcc attttctatt ctccttgtaa gtggttcaat cgattttcat ctcccacgtc
     1021 acttctcgaa atgatgaagt cttgcgggtt ttcagtctat tagattcttt tcctacggcc
     1081 ttaagctccc ttgctgtacc caagtacaag catcagtcac ttgcctagta tgaaaagcaa
     1141 ctaatagact gctgtgagat tttctactgt gttattgatt agtcaataac agtgaatccc
     1201 atagaacgag cagtaccttc gatcatacgc actgcagact ctacgtttgc tgcgttcaaa
     1261 tctggcatct tagtttctgc aatttcttgt acttgtgcac gagtaactgt agcaacttta
     1321 gttttattag gtgtacctga tcctttttca acacctgcag cttttttcaa aagaacagca
     1381 gctggtggtg ttttcgtaac gaaagtaaat gatttatctt cgtaaactga gataacaact
     1441 ggaatgatca taccagcttg gtcagctgta cgagcgttga actcttttgt gaatcccatg
     1501 atgttgatac cagcttgacc aagagcaggt ccaaccggtg gagctggtgt agctttacca
     1561 gcagggattt gcaattttac aagtttttcg acttttttag ccatttttaa atcctccttt
     1621 gtggttttgg cggtaattag agatttttac ctcccacaag tatgtttttc acatacccat
     1681 ctattataca ctatttatct ggaattgcaa gagaaaagtt tattttttaa tcgacgtaat
     1741 ctccaccttc aaagccgaaa tactcttcca aactgaggcc actagctgca atctctttag
     1801 cctcttttcc gacataacga aggtgccatt cctcagccat atagcctgtt tccttttcct
     1861 tacctttgag ataacgtaca acaaagccat aatcagccgc atgatccaag agccattggg
     1921 ccgctttttc ttctgtcacc aaatcaccat ttgtcccaat cacatcaaag gctaaacctg
     1981 tctggtgctc gctatagcca ggacgggcag aataacggtc ggcagcttct ttcccatctt
     2041 gattgacata atcttgatag agcttagtct gagtttcata acttctaaag ccactgtaat
     2101 gatcgctaat ggggaaacct gcctcttgca tagctttgat gagcttgacc aactcagcct
     2161 tggctgttgg attttcccct ggattgtagt cttttgacaa tggatagtgt ttgttggcta
     2221 cgatgatttc atcgtatttc ccttgaatgc tgtaatagtc tcctttgtta accacttctg
     2281 cttttttctg ggcagctcct tgagacttac ttcctactgt tccatcagct ttagcagttt
     2341 gctctgtctt agctgctcca tcttcatttt ttgctttttc ttgtgaacaa gctgctagac
     2401 tcaaggctag caaggctgtc aagacaaggt atcttttttt catctttact cctttatttc
     2461 taatagttta tctacttctg atgataaacg ttcgactaat cttttaatct catcaggttt
     2521 tgattggcag atttctgctg tcatttcttc aaagggaatc caccatactg gaccacgtcc
     2581 attgaggcca taaccattca tatcacctct tcgtctagga aacagatgcc aatggagatg
     2641 agcatcgcca tttcctagta gttcgatatt cattttctca gcagcaaagg ccttggcaac
     2701 tgcctcttgg accacactca tttcttctag aaaacgtatt ctagttttct tttctaaatg
     2761 atgcaattcg ctgacatgtt ccttggctag aaagagacta tagcctgcaa aatactggtg
     2821 gtctccaatc acaagatagc ctgtttccaa ctctttgaca aaatagggat tttctccctt
     2881 cttgatgagc tcaattctct ggcaaatcaa acacatatta gtctaccaat gcactcttaa
     2941 gataggcatc gaccattcgt tctgttgcga ccaccgagtc aatatgagtg cgctcataag
     3001 aatgactaga ctcgataccc gcaccaagaa gggcatgctt gacctctgcc cctgcagaca
     3061 tagccgctga agcatccgaa ccataaaatg gatagatgtc cagcttaaaa ggaatatctt
     3121 gctctttagc caaggcaacc aagtgttgac ggaagtcata gtgataagga cctgaggcat
     3181 ccttgacaca gatagatact gtgtactcat ttgtctgctg gtcatctccc atagctccca
     3241 tatccacagc cagatattct actacctgag caggaatgtt agaattagca ccatgtccta
     3301 cttcttcaaa aactgaaaaa gcaaaatgag ttgttactgg caattcaatc ttctctttct
     3361 tataaatacg aagcaggttg agcaacatcg ctgcgctgac cttatcatcc aaatgacgag
     3421 acttgataaa acctgtatca gtcacaacag ttcgtggatc gaagctgata aaatcaccga
     3481 cctcaatccc aagagcacga gtttcttttt cattgcttac ttttacatcc aaacgcactt
     3541 ccatattgtc ctgcgtgcgt tctgcagttc ctgcatcctt gtagacatgg caagaagttt
     3601 ggtggatgag gatggttcct aatacctttt gacctgtgct agccacatga acggtacagt
     3661 tttcaccttc aatcatgttc caaggaaaac caccgatacg gtccaattta agacgaccat
     3721 ctggtttgac agcacgaaca atagcaccca gtgtgtccac atgggcagtc acatagcgat
     3781 gttgctcatc attttgacct ttgatggtca cattgacacc acccttggct gtgcgaactg
     3841 gctggtaacc aaaaccttct agagtcttga ctaaataatt cgaaatttca cgagtgaagc
     3901 ccgttggcga tgcgatggct gtcagttctt tgatatattc tactgtttga ttcatttctt
     3961 cacctctttc gctacctatt ataacacatt tatcatcgga taaaaaaaga aaatcactcc
     4021 tgtagacttg gctacaaaag tgatttaagt gattattcca tttcaaaaac atcgctttct
     4081 tgcttgtttg gtagaaccaa tgagagcaag attccaacaa tggctggcac caaccatggg
     4141 agagatgcct tggcaaaagg aagaacgcta acaagatttg caagaaactc aaccttaaac
     4201 gagcttccta gtacgcttgc aatggcaata actgtaacca cagcaattgt caactgcata
     4261 cctggttttg aaagggcaac aaatttgttg acaatgacaa tcataacgat agcaatcgtg
     4321 attgggtaca agataaccag tactggaatt gagtacttga taatcgcatc aagacccaaa
     4381 ttggcaatag caaatccaat caaggtaaag gctgtcgcat aaaccttgta gctgatttgt
     4441 gggaagcgct cattaaagaa ctcagctgtt gacacaatca aaccaacagt cgttgtgaag
     4501 caggttacgg taaccatagc tgcaaggaag agttgagctg ttgagccaaa gatttcttga
     4561 gtggcttgtg acaagatgta aacacctggt gttccaccct tcatcgcttc agctggtact
     4621 gggaaatgat ttccaagaaa acctaaaccg atgtaaagag cgctgaaggc aagggcaaca
     4681 acgataccaa caacccaaat agttgaaatg tattctttct tacttgaaaa tccaagttgt
     4741 ttcaaggttt gaactgcgat tacgctaaag gccactgagg caagggcgtc caaggtatta
     4801 taaccttcta ggaaacctgt accaaaggca gaagcttgat aagcagctga agcagcttga
     4861 ggacttgttc caccatattt gatagctccc agaacgacca agataacaat caaaattgca
     4921 aagactggcg ttaaaatacg tccaatgcgg tctaagattt ttgatggatt aagcgaaatc
     4981 aaataggctg ccgcaaaata cagaaccgta aatacaatca agccaagtcc tttatttgca
     5041 tccgacaaaa gggggctaat ccctacttcg taagctgttg tagcagtacg tggggtagca
     5101 aagaatggac cgattgacaa gtaaagaact gagaggtaaa gagtcgcaaa ccaaggcgct
     5161 atcttcgttg aaatctcgta gatatatcct ttaggattta gcgttccaat aataagggtc
     5221 aagacggcga taccaacgcc tgaaaagaca aaacctgcga tggcaggaag aaaatgttct
     5281 ccagatagag cacctagaga aggcggaaaa atcaagttcc ccgcaccaaa aaatattcca
     5341 aacaggagca aacctgttag ggtacctttt ttagccataa aaatctcctt catatttata
     5401 aaatactgat ctagtatacc acgattagga gtctgaaaaa agcatcacga ggcaaatatt
     5461 aaatgttttc aattttctta aaaataagaa ttatctaaaa ataaaactct cctacccagt
     5521 ccacaccaag taggagaaat gcaaaatttt aaagttcttc aaaggctaac atgccaccct
     5581 ggacattgat aacattataa ccttgttcta ataggaattg gcaagcacgc gccgatctca
     5641 ttccagattt gcaaataata taatgcaagc gatctttgtc caattaatca taactatcag
     5701 ccaattgact aagcggtagg ttgtgggcac cttctaaatg aagagctgca aactcatcca
     5761 cttctctcac atccactaga gaaagctggt cgttttggta aagctggtaa aatgcgtcaa
     5821 aggctatttc tttcattctt tttcttctaa tactcttcga aaatctcttc aaaccacgtc
     5881 agcgtcgcct taccgtgcgt atgttaccga cttcgtcagt tttatctgca acttcaaagc
     5941 tgtactttga gcagcctacg actagcttcc tagtttgctc tttgattttc attgactata
     6001 aaatggtttt aattcttttt ttcaaatctg gcactacttc tgcctcaaac caaggatttt
     6061 tggccatcca gatttgattt cgtggtgatg ggtgaactag cggaaaatag gctggcagat
     6121 agtctttata gtgtttcacc ctctccgtta ccttcccact gattttctcc tgtaaatagt
     6181 aggcttgggc atattgccca atcaagaggg ttaactgaat atcaggcaat tcctgtaaga
     6241 gctgcggatg ccatttttct gcaaaacctg tacaaggcgg aagatcaccc gacttgccat
     6301 gtcctggaaa gtagaaatcc ataggcaaaa cagcaaaata acctgaattg taaaaggtat
     6361 cttcatccac acctagccag tcccgcaaac ggtcaccact tttatctttc cagtaaaggc
     6421 ctgcttcttg agttttaagt cccggagcct gaccgatgat attgatgcga gcagtttttg
     6481 gcgctgcaaa gagaggctca atgccacgct ctgtatagct ggcattctgc gaatccgcca
     6541 tgatagcctg tttgattctt tcgatttgag acatctactt tccctccaaa aagaagtcca
     6601 agcataagat ctcagacttc tatcgtttta tttgatcaat tcgtaaatag cttcggcata
     6661 aattgctgct gctcggaaga gatcgtccaa ggcaataaat tcattggctt ggtgcatggt
     6721 atcaattgag tctgggaaca tagcaccata ggcaactccg cgctctaaca agcgaccaaa
     6781 ggttccacca ccgatgactt gttcatgacc tttaaggcct gtttgttttt catagacatt
     6841 caacaaggtt tgcacaagtg gatcttccat tggcacatag tgaggggtat gaccatgttc
     6901 tgatagactg acagaagcaa ctggcaagtt ttctaggatt gacttgattt gttctggact
     6961 tgttcctttt ggatagcgga tgttaagggc aatggtatta tcagcacttg tttcatcgaa
     7021 gcggaagacg ccggcattca tagaaagagc acccatcttt tcatccacat gagcaatctt
     7081 gagattttca ccctcatgat cgttcaagag aattttacct gcgatgtcaa ggtagtcttt
     7141 ggctggacca gcaaagccaa actggctgag gaagagggca aggtaagttg cgccattgac
     7201 acctgaagca ggcatagcac cgtgggctga tttaccaatg atcgtcacct tgtattggcc
     7261 agcttcttct tggagttctc ctctaagttt gtgttctgca acaaaggcat ctagtttagc
     7321 ttgcaagtct gtcaagtcac ctgaaacgac tgctgttgct gattctggta ccatattttc
     7381 acgtaaacca cctgtaaagc tgtgaagacg ggctgcacct gtattttcac ctgcaaagtg
     7441 gaggtattcc gtgatgtttc ctttttcacc attgatgatt ggaaattcag catctggtga
     7501 gaaaccaaag tctggcttgg cgagtcctac gtgctcgaag tagtagtcca tatctgccca
     7561 gcctgattct tcgtctgttc caacgatgaa gcgaactttc ttagaagttg gaaggcccaa
     7621 ttctttgatg attttcaaac catagtagca agctgttgta ggtcccttgt catctgaagc
     7681 accacgcgca taaaggcgac catctttgat agttggtgtg taagggtctg tgtcccaacc
     7741 gctaccagct ggaaccacgt ccatatgggc aaagattccg agaacttctt ctccatcacc
     7801 aaactcaaaa tgtcctgcat agttatcaac atttttagtt ggataaccat cacggtctgc
     7861 gatttcaagg aatttttcca aggcttttac tggaccaggt ccaaatggat gttgggcatc
     7921 agccttgcta tcatcacgtt ctgagttgat ttccaaaagg ctaaacaagt cagccaagag
     7981 gtcttctttg cgtttttcta cttctgctgt aaaatcaatt gctatcattt tttcttcttc
     8041 ctatcttttt tcgatgattt catctactgg caagcggtag cttggttcca atttgtcgtc
     8101 tgtgtaaccc actgtgatca agagttctgg gcggaaacgg tcttcaattt ccaaaacctc
     8161 attgactttt gatttgtcaa aaccaaggat aatattggaa ccaattcctt ggtctgtaag
     8221 agcgagaacc aagttcatag caaccaaacc tgcattgagt gctaggtagt cgctgacttg
     8281 ttgttcattg tagcgtgcaa attcagctgg caaatttttc atgaagtatt gaagttgttc
     8341 ttctgagaaa ttgttagcac caccaactcg ggcaatctta cgagcgcgtt tggcaaggtc
     8401 tgtatctgta aatagggcaa tggttacagg tgctgatgat acctgctcaa agtttgaacc
     8461 ataagccaac tttgccagtt cagcattttt ctcacgaaca actacaaatt tccaaggctg
     8521 gctgttgtgg gcgcttggcg ccaaggttgc aatttcgata gccgtacgca catcttttag
     8581 atcaacaggc ttatcagtga aatgcttggt cgcatgacgt tttttattta attcaagaaa
     8641 tttcataatc gatttccttt tctaattcta ctgcttccat tctaccataa tttgaaagag
     8701 cttgcaggga tttttcatga ttaagttttt ctatcacaga cataaaagat atatattggt
     8761 ttatcaggaa agtttctgat aaactagcaa gtactttaca tttgaatgga gacagtatga
     8821 aaaaatttag cctattacta gctatcctac catttttggt tgcctgtggg aatcaagcta
     8881 cacccaaaga gactagcgct caaaagacaa tcgtccttgc tacagctggc gacgtgccac
     8941 catttgacta cgaagataaa ggcaatctga caggcttcga tatcgaagtt ctaaaggcag
     9001 tagatgaaaa actcagcgac tacgagattc aatttcaaag aaccgcctgg gaaagtatct
     9061 tcccaggact tgattctggt cactatcagg ctgcggccaa taatttgagt tacacaaaag
     9121 agcgtgctga aaaatacctt tactcgcttc cgatttccaa caatcccctc gtccttgtca
     9181 gcaacaagaa aaatcctttg acttctcttg accagatcgc tggtaaaaca acacaagagg
     9241 ataccggaac ttctaacgct caattcatca ataactggaa tcagaaacac actgataatc
     9301 ccgctacaat taatttttct ggtgaggata ttggtaaacg aatcctagac cttgctaacg
     9361 gagagtttga tttcctagtt tttgacaagg tatccgttca aaagattatc aaggaccgtg
     9421 gtttagacct ctcagtcgtt gatttacctt ctgcagatag ccccagcaat tatatcattt
     9481 tctcaagcga ccaaaaagag tttaaagagc aatttgataa agcactcaaa gaactctatc
     9541 aagacggaac ccttgaaaaa ctcagcaata cctatctagg tggttcttac ctcccagatc
     9601 aatctcagtt acaataacac tcttcgaaaa tctcttcaaa ccacgtcagc tttatctgca
     9661 acctcaaagc tgtgctttga gcaacctacg gctagcttcc tagtttgctc tttgattttc
     9721 attgagtata agatatacta aaagcgattg gaccagccaa tcgctttttt agtttgcatt
     9781 ggctatttta ggaaactctt acaaaaaatc aaaaaaattc ttcacatcct ctacataccc
     9841 atgcttttct attaagctgg ctaaaagttc cagattgtgc ccctgatagt tatcgtcacg
     9901 acgcagttct tcatacattt caataaaggg aagccccttg gacttggcca attctaactc
     9961 agcctcgtaa tcataagcaa ctttacgaaa ttggatattg accaattccc catcttcaac
    10021 ttctatcacg gcatactggg cacggtgatt ttttaacgcc tcccaattaa aatagggcat
    10081 gccaatcgac cctggattga tgatttgttg cccttgactt ccataacgaa gcaactgctt
    10141 gtgaacatga ccataaactg ccacgtccgt ttccgcatct agcagttggt caaatttctc
    10201 tgtatcattc tcaactagca agtcaccacc atagttctta tttggcaaat tatgagagat
    10261 agaaaagcgc agtccagcaa tttctttctt ttccagcaaa ggcaagcttc gtagccagac
    10321 agtcgttgca ggatccattc gctccatcaa aaactgagtc attcgcatga gctggatttc
    10381 ctgtgggtct tctaagccat attgcccatc taaagcctca aggacacaat cattccaatt
    10441 gcctcgaaca cttgccgtga taggaagttc ctttaacaga gcgaccaagt catttgcacc
    10501 tgaaccaggg aggaaaatgt ctcccatgag ccaatattca ctggctcctt gagttttagc
    10561 atctgcaatc actgcttcta gcgccgtcgc attgccatga atatctgata aaattgcgat
    10621 tttatggttt attgtggttt ccttccgttt ggtaatctac tctttcttcc gccacttgaa
    10681 gcaagatttc tgtttcctgc gggttcaact ttctttgcac agcctctgct actgctcttg
    10741 gtactccaac ttcgacaatc tcatccacac tggcttcctt gattttggtc agagacttga
    10801 agtgcctcat gagattttgc ttgcgtttag gtcccagacc atcaatccca tccaattgag
    10861 atgagaaaga atttttggag cgcagttggc ggtggaaggt aatggcaaag cggtggacct
    10921 catcttgaat gcgttggagg aggaaaaatt cctgagaatt gcgagacaag tccaccactt
    10981 ccagcggatc tccaaagagc aattcatggg tttggtgctt atcattcttt tgcagacctg
    11041 caattggaat atccaagccc agttcttctt ggatgacttg cttagcaata ttgacttgac
    11101 cttgcccccc atcaatcaca atcaaatctg gaggagtcaa agcctcacgc tgtactcgac
    11161 catagcgtct tcgaatgact tctctcatac tagcatagtc gtccggccca acaacggttt
    11221 ttatcttgta cttacggtaa tcttttttac ttggcctgcc gttaacaaag accaccatgg
    11281 ctgaaacagg actggtcccc atgatgttgg agttatcgaa ggactcgatg cgaactggag
    11341 tcggaatttg gagcaaacgt cctagatttt caatagctcc ttgggtcttt tcgactgatt
    11401 tttctagcag gttaaatttc tgctccagac taacacgtgc attttttatg gctagattga
    11461 ccagctgttt tttctctcca cgttggggtt tgagaatctt ggtatccacc aaagccttga
    11521 cagcttcttc gtcaatatcc tgcggaatca gtacctcatt gggaactagg tgagattttt
    11581 cttgatagaa ttgtcccaca taggtcaaga aatcctcatc tggatcattg aagtagggga
    11641 agagattgac atcgcgctcg atgagcttgc cctgacgaac aaagaaaacc tgcacacaca
    11701 tccagccctt atccacatag tagccaaaga catcgcgatt ttggagatcc ttagccatga
    11761 cccgttgctt ggttcgaagc gttccaatag cctgaatcag gtcacggtat tccgccgcac
    11821 gttcaaactc catactttgt gctgctactg ccattttact cttgagatca tcgatgattt
    11881 tgtcatcctg acctttcaga aaatcagaca cctcctgagc catagacttg aaataagcct
    11941 catccttctt acagatggtg tgggccatac actggccgat atggtaataa aaacagacct
    12001 tagagggcgg gttggtacat tttcgaaaag ggaaaatcct atctagaagt cgcttgattt
    12061 cattggctgc ccccacatct ggataagggc caaaataaag accaccgtcc tttttgacct
    12121 gacgagtaat gataagccgt ggatagcgct cattggtgat tttgatgaaa ggataggact
    12181 tgtcatcctt gagcatgata ttgtacttgg gcttattttc cttgatcaag ttgatttcca
    12241 gcagaagcgc ctcaatattg gactcagtga cgataaattc aaaatccaca atttcagaca
    12301 ccagagcctc tgtcttggta tcatgaattc cacgaaaata ggaccgtact cgattacgca
    12361 gatttttagc ctttcctaca tagataatgg tgccattttt atccttatga atgtagcaac
    12421 cagggctggt cggcaagagc tctagttttg atttaatcaa gttgttcata gttccattat
    12481 agcaaaaaaa gtagggaaag atagtaccct actcactagt ctcatatatt tttcggaggt
    12541 ttgcaacatc ctcttcctgg ataccataaa aggaacctgc tggttgcatg acagacttct
    12601 catctgtatg gtccaagcca atcgcatgac ctaactcatg ttctgccgta tggacaaggc
    12661 gttcatagga gtagccatag tatggattgg acaaataata atgattcaac cgcaccgtta
    12721 cggacaagaa ttgccctgtt aagagattag tttgactttc cgcctctcct gccacaggag
    12781 tgcctccgtc gttcatctcc gtagccgtaa tatccgcctt actagactca gtcacgagtt
    12841 caaagttaaa agcaccagtt tgattccagt tctggattgc ttctaaataa gcctcttgaa
    12901 agcgtgaatc catctgcgga tccaggtaaa tacgagcaga ggcctgtgcc caccttcctt
    12961 cagaatgctg gtgactatct gtctggctta acttaggcag ttgccctgtt ttctcccatt
    13021 ggtcaatact ttgttgaccc atttttactg accgttctgc ttgctttagc gctccttgaa
    13081 aatccccgtt cagataccag agaagtccga aagcaagcac acataagagc acaactatcc
    13141 aaaccagacg ccaaaacaaa cgccacacaa aagaaaagaa agcccctatc aaacgaaaaa
    13201 gccagcgcat gtctctccac ctttctagca aataagaatt attttactaa aagaagctaa
    13261 aagaaatcta aatactgact ttttcatctt aacgtcctac tatttttttg agtaactgga
    13321 tagctttgta ttttaaaggc gatggatggt aacggaaagt acctgctttg cgtacaatat
    13381 agccattaaa attctgttta aaacgcaaaa caccatcact tccatcaaaa atcccttgaa
    13441 tgcctaggaa gttgtattta ggtattccac gttttatgct ttccaacata acatattttt
    13501 gaagcagtgc aggggcatag aacttattaa actcagtgta ggaaccacta aagagataag
    13561 tcgtttcctg aggcatataa acaaataaac tcccagctaa aacaatatct tcttctccat
    13621 atttttcaat caagtctcgc gcttctgctt ttcgaacttc aaacgtttca aattgactag
    13681 aatattctct cagttgattt tgttttttct cagaatgagg atttttactc aaatcaagtc
    13741 gcaacttgtc caagttttct tctagtttac tttgttcacc ttgcaatttg ctcatatact
    13801 ccgaaaaatt caagcttgct atgagaaact ccgcttgttc tccaaaagta tcataaaaat
    13861 gctcataata ttctaaactt ttatcactat attctctacg ttcagaggtt tcttttgtta
    13921 tattcttaaa aatcgatagt tcttcacgtt ttaacttttt caaccgaatg ccaaaggttt
    13981 cagccttttt caccaagggt ttaccctttt tgctaaaact tttaagcaaa ctcttttcag
    14041 ttaattcagt taaatcttta tagtataacc aatctggttc tccacctggg taacctgttg
    14101 ttaagccatc aaattgataa cctaaatcag tcaaaccttg aataatactt tttttctcag
    14161 catctattgg attaccttgg ctatcaaaag tttgataagt ttcataaggt tttacaagca
    14221 actctaatac accattttgc ttggcatatt cttttaactc tgcataaaaa actggaagag
    14281 catcttgttg ggtataaatc ggccccgaat tgagttccat atgcagacca cccagcatgg
    14341 gcaagctata aaccagagct gcaacttgaa tttctccttc ttgtttcaaa gcaagataaa
    14401 caattcgagc ccctcttttt tctagcaaat cccccatctg gacagattgc ataaaggaac
    14461 gagaagaaac ctgatcagaa taagtctgaa actcttcttt cgtgagtgtt gttagtgcca
    14521 tatacttact ttctatgttt ttttcttaat gttttacgga aatcaagagc aagtcttaac
    14581 agaggataga gaggatgagt gggcattgta aattcaccca agtattcttc aatcgttgga
    14641 ttaaattttt ccttaaaatg ataaagtcca ccattgagag agttttcaac accacctaaa
    14701 ttttgccaga ccatacctcg ctcaaaggca tagcgagccg tttcatacca tgttaaaatt
    14761 ggtgcattgt aacgtttaaa atcatcatcc ataccagcat atatattgac agaggtagta
    14821 ccaaattcca aactcaaagt agccgctaaa ggaactctcg cttgacctac atctatatat
    14881 tcctgcaaga aggtcaattc ctctaacaaa cgttcttttt ccttcttctg cgcttctact
    14941 tttgaagttc gagtcgactc agtaaaggtc tcttccaagg ctctattttt cgctaactgt
    15001 tcttctaact cttgcgaacg tttagaaaca tccaaggtgg ccaaggtgat ataggccttg
    15061 tccttaaaat tatctaacaa ttttttataa taggcttcat tcctcaaatg aatctctttt
    15121 cgcttctcag tttttttcat caactccgaa aatgaatcta atagttccag tccaccatat
    15181 tgaatctcaa gccctttgtt tcgtgctgtt cgaatagcct gttttgttga cttggaaagt
    15241 ttatcttctt caaaattttc cttgtatatt ttcgcctgaa tacgaggttg aatggtgtct
    15301 cccatttcct ccgtttttcc tgaccacctt actcccattt gttgcaaact atcaataata
    15361 gccagatttt caggaaattc tgtcttttcc tgattgatta aactttgaga taggcaaata
    15421 cttgggtcaa aagtcacaaa aaccgctctc ttactgcgag cataggactt aatagactga
    15481 atggcaaaat tcaagagttc tttatcccca taatccaata taggtcctct tgggatgtaa
    15541 aacattttat agcctagcgg aagagttcta atcaaaatac tagctgtcgc cagtaatttt
    15601 tcttccctgt aaacaccaaa cttctcatgt tgccaattag acttaacttc ctcccaagca
    15661 ctactttgta atacattggc taattcatgt tctttgacaa actgatcata ttctaatgtg
    15721 ggaatgccaa tttgataacg gtacatttcc tactctcttt cctccagtat tttattacta
    15781 ttctactact ttcgtttgaa aagtctagtg aaaacatatt ttattttagt tctactcccc
    15841 ctatgctgaa gttcaaacta agtcagtctc tcttctaatt tgaaatcaat tggtagggtt
    15901 gttaaaaaaa cttccaattg tttttcccag tagtaccact cgtgagttcc agcgctatgg
    15961 ctataggtca catcaaaacc tagttttttg agatttttca ctgcgagatt attggcttcg
    16021 tacaagaaat cctgttcgcc acaccacgcc caaagtttgg tctttttatc cgatttttta
    16081 gccagacttt caagagaata gggactagtt gtccagtctc taatctctcc aaaaacacct
    16141 ctccagtagg ctggacttcc cagattttga ctttcaggag aaaagttttg aaagctgagg
    16201 gcacctgaaa aactagctgc atgagaaaaa cgatttgtcg caagagccag tttgaagcag
    16261 ccgtaacctc ccatagaaag accagcgata aaggtctttt cacgcttgct agtcatatta
    16321 gggaagaagc gtttcagaac ctgtggcaat tcctctgcta gagccgtgta gtagtcaaaa
    16381 ccatactggg tatcggtgta ccaaccattg ctggtattgg gcataacaac gatgagatta
    16441 gttcctcgaa gcaagcgttc tacattggtc cgcttaagcc aactattatg atttccagac
    16501 atcccgtgca aaaggtacaa gacgggaata tcttcacact ctggttcttc cactcgattg
    16561 gcatcagggt agaggacatt caccccccac tccatatcca atacttgtga gtaatactcg
    16621 attttcatta ctgccatgat tttctccttc tatttttcta taagaaaaag ccgaaactcg
    16681 actttctctc tgtttatttc aacgattatt ttgcttccat gactactggt aaaatagctg
    16741 gacgacgctt ggtttggtca aagagatact tggtcaaatt atctcgaacc ttccctttaa
    16801 gatctgccca gtcaaagtca tctccttgaa gataatcttc taccgtttgg ttaatcaatt
    16861 ctgaactttc acggagaata tcgcgactct tcttgagata aacaaatccg cgcgtatgaa
    16921 cacgagccct agccacaatt ttcttctcac gacggttgac tgtaatagcc acgatgaaaa
    16981 ttccatcctc tgacaagatc ttacggtcac gaagaacaac atttccaaca tcaccaatgg
    17041 cattcccatc aatcaagata tctcctgctg aaaccgatcc agctggaaca aagtctccat
    17101 tctcgtaagc catggtcgtc ccctttttag gaatgaagat gtgttctggc aacatcccaa
    17161 ctgccatggc agccttagcg tgagcatcca actcacgata ctccccttgg acagggaaga
    17221 ggtacttagg ttgcaaaaga ttgatcatca gctgcaaatc acgcacattt ccgtgccctg
    17281 atacatgtaa actttgggta atcaatttga caaccccacc tgcctgataa atcatatttt
    17341 ccacacgcgc aacaaaggct tctttagcaa tagacggagc cgtagcaata tagaccaggt
    17401 ccccatcctt gatttctaca taacgatggc gaccaatcga catcttacga agtccattga
    17461 taggttcacc catacgacct gtctcaagaa taatcaactc atggtcttca aagcgagaca
    17521 tatctttagg cttaatcaag agaatttcgt tggctaaaga caacttctta agacgaatcg
    17581 ctgtgcggac gatattttca atatcaaatc ctgtcaagac gatacgtcga cctgttttat
    17641 ccgcagcgtc aaaaatctgc tgaatacgag aaagattact ggaaacagct gcaacgatga
    17701 tacgaccttc ccagtcagca atagtttggg taatttcatc cctaacttca ctttcactag
    17761 ccacctgaat attgctgtct gcattggccg aatcactgag gagagccagg acgccgtcac
    17821 gaccaatctc tgccaaacga gcaaaatcag ttgcataaga ttcactagcc gtttggtcaa
    17881 atttgaagtc acctgtataa acgatgcttc cttccgatgt cttcaagaca attcccagac
    17941 tctctggaac ggagtaagtc gtagggaaga aggaaaccac tgtcccacca aaatcaatct
    18001 ccgtattctc atcaatgaca tggaaatcat taaatttctt aacggcatca tttcctttga
    18061 caaagagctt tgccaactca atggtcaact cagacccaaa tacaggaact ttagcctctg
    18121 ccaagagata aggtagagca ccaatggcat ccgcatgccc gtgggtcaag aaaaccccag
    18181 caatacggtc gctattttca aaaaggtaat ccatgtttgg aatcaccaca tcgaccccta
    18241 attgttcatt ttcaggatat tttaacccta cattcaaaac aaaaatggac tctccaattt
    18301 cagcaatgta catatttttt ccattctcac gcacaccacc aagtgttgtt aaactgatat
    18361 tactcatttt tcctccaaaa tcttagttta tcttgattat agggcaattc taccctctta
    18421 ttctaaaagt tctgatactt tcagccgaat cttttcctac cattatacca tttttttgat
    18481 gattttcaaa atgaaggtct gttttcaaaa aagagttgat ttcccaactc tttcctaact
    18541 tctactcttt ttccatcaaa aaatcataaa tttcttgcac cgtacccagc gccatcattt
    18601 cttggtcttt aacggtttgt ttgagatttt ggtaaatttg actttctcct atctcccgct
    18661 ttggcgcctc tttattgact gtcatcacac catcttttat cgtcacaaag cctagttctt
    18721 caaatacttg aatcatcttg accagcaaga tttgttgaat attaagatat gcagccaaat
    18781 ctttcagctt gtagcgaata tcaaactctg ggaactggta aatagtcttg tacaatttag
    18841 caaactgatc tctagtccca taacctgtca gataataggt cttgtcaata tcatttttga
    18901 aatagacagc agagaaatgc tgttcctgaa aaatggtctt cagctgagta atatcctctg
    18961 gaatgttttt tacgacaaca gcttcactag ccgcaagatt tggcagttct ccaggaaaat
    19021 ccaagactgg aacaccttct ggcaagactg cattttttcc acgaatgtta aaaagttgaa
    19081 caccttccac tcgcgcatcc accatcatca actggagggc agtttggcca ttccattggt
    19141 tgacagacaa tttaaccgct aactctagat tcttggtttg agaaaactct gtcgcccatc
    19201 tgccttgacc aaaggctacc acttcaaaac tcgcctcacc cttggaaatt ttcagcttta
    19261 gatgggcatt acctgccccc atagtacgag cactttcgac ctgaaaattc ttgatataaa
    19321 aaataggttt ctgattatcc attccaaaag gagctaaacg ttcaaaactt ttgaccgttt
    19381 ccaagctaag tgcctccaaa tccaactctt catctaggtt taacttattc ttgccaccag
    19441 catctgcacc tttttcacga acataatctt ccaaaacctg agataaatct gagagttgct
    19501 caacttccag cgtcataccc gctgcacctg catgacctcc aaaggcgatg aagaggtctc
    19561 gatggggatc cagagcttca aaaatatcga ccgcttccac actacgagca ctgcccttgg
    19621 cacgaccgtc ttctatatta agaacaatga ctgtctgtcc caattcttcc aataaacgac
    19681 cagccacgat tcctagaacc ccaggattcc agccttcctt ggccaagacc tgaaccttct
    19741 tctcaggatc caccatggtc ttggcttctt catagataga ctgaacgatt tccttgcgct
    19801 cttcgttttt ctggtgaatc ataagggcaa tctcatgcgc ttcctcatca tcaaatccag
    19861 tcaacaaatc aatggcagga ttgggatcat ccaagcggcc caaggcattc aaacgaggag
    19921 caatctggaa accaaccgtt tcttctgtta cttcgttggc agcaatccca gccatgtcca
    19981 gcatttcttg cagaccaatg cgctgggtat gacccaacat ttccagacca tattgaacta
    20041 agatacgatt ttcatccgtc agactcacca tatctgcaat agttccaata gcgaccaaat
    20101 caagcaattc cacttgcact tcttctaaca gggcacaagc caacttgaaa gcaactccac
    20161 aaccagccaa atatttaaaa ggataatccg catctggatg ttcaggatgg acaatagcat
    20221 aagcatctgg cagggtttca ggcatggaat gatggtctgt cacaatgaca tctactccca
    20281 tagactgagc caatgcaata gcctcatgac cagcaacccc attgtccacc gtcacaatca
    20341 aggaaattcc ttcttgctcg ataaagtatt tataaacact agcattaggg ccatagccat
    20401 cggtaaaacg atttggcagg taaactcggc actcagcacc aagttgttcc aaactttcct
    20461 tcacaataga agccgaagtc atgccatccg catcatagtc tccataaacg agaatatttt
    20521 ccccttcttc aatagcctga cgaatccgct ccactgcctt gtccatatca tggagcagat
    20581 aagcatcatg taagtcctcc aaggaaggtt ctaaaaactt cttcagactt tcttggtcct
    20641 gaatccctct ctcaaacaat aaccgagcca cctcaggacc cagtccagcc ttcttggcta
    20701 tctttgtaaa atccgcatct tctacctgca gggcaaactg ccattcataa gtaggtgtta
    20761 tcaaaaagac atccactctt tctaagaatt ctattgcttc attataacat gatttagacg
    20821 ttttctctat ccagattgct tttttataac attttatagt atattgaaac tagaatagta
    20881 cacctctact tctaaaatat tgttagaaat cgatttgact gtcccgatcg atttgtcctg
    20941 ttcttatttc actatagtga attttttcag atatggtttg acaagaacat tctttcattg
    21001 tagaatcgtg ttataaaata taacacaaag gagatctctc atggctttag tagaatttaa
    21061 aaacgtcgaa aaatattacg gagactacca cgcactccgc aacatcaatc tccgttttga
    21121 aaaaggacaa gttgttgtcc tgcttggacc ttctggctct gggaagtcca ctcttatccg
    21181 tacgatcaat ggtttagagg ctgttgacaa aggaagtctc ctagtcaatg ggcaccaagt
    21241 tgctggtgcc agccagaaag atttggtacc tcttcgcaag gaagtcggca tggtttttca
    21301 acattttaac ctttatccac acaaaacggt gttagaaaac gtgacacttg cgcccattaa
    21361 agttctagga attgataaaa aagaagctga aaaaaccgcc caaaaatatc tggaatttgt
    21421 aaatatgtga gacaagaaag attcctatcc cgccatgcta tctggtggac aaaaacagcg
    21481 gatcgccatc gctcgtggtc ttgctatgca tccggaactc ctcctctttg atgaaccaac
    21541 atctgctctt gatcctgaga ctatcggaga tgttctagca gttatgcaga aactggcgca
    21601 tgatgggatg aacatgatca tcgttaccca cgaaatgggc tttgctcgag aggttgcgga
    21661 ccgcattatc tttatggccg acggagaagt tttagtagat acgacagatg tcgataactt
    21721 ttttgacaat ccaagcgaac ctcgtgccca acaattcctc agcaaaatta tcaaccacga
    21781 aagtgacaaa gtcaaataag gaggcgccta tgaaaaaaaa ttctttttat cagcattatt
    21841 gattagcctt ttcggccttg ctgctgccaa accagtccag gctgatacta gtatcgcaga
    21901 cattcaaaaa agaggcgaac tggttgtcgg tgtcaaacaa gacgttccca attttggtta
    21961 caaagatccc aagaccggta cttattctgg tatcgaaacc gacttggcca agatggtagc
    22021 tgatgaactc aaggtcaaga ttcgctatgt gccggttaca gcacaaaccc gcggccccct
    22081 tctagacaat gaacaggtcg atatggatat cgcgaccttt accatcacgg acgaacgcaa
    22141 aaaactctac aactttacca gtccctacta cacagacgct tctggatttt tggtcaataa
    22201 atctgccaaa atcaaaaaga ttgaggacct aaacggcaaa accatcggag tcgcccaagg
    22261 ttctatcacc caacgcctaa ttactgaact gggtaaaaag aaaggtctga agtttaaatt
    22321 cgtcgaactt ggttcctacc cagaattgat tacttccctt cacgctcatc gtatcgatgc
    22381 cttttccgtt gaccgctcta ttctgtctgg ctacactagt aaacgcacag aactactaga
    22441 tgatagtttc aagccatctg actacggtat tgttaccaag aaatcaaata cagagctcaa
    22501 cgactatctt gataacttgg ttactaaatg gagcaaggat ggtagtttgc agaaacttta
    22561 tgaccgttac aagctcaaac catctagcca tactgcagat taaggaggac accccatgat
    22621 agatttatca tcttggacag cctattttca ggattttgga caatttttca atggtttcct
    22681 ctttactctt gccctagcga ttggatcctt tatcctcgcc atggtcttag gaatcttctt
    22741 tggtgccatg tcaaccagta aacgtccaat tttacgcatt ttagctcaca tctttgtgga
    22801 attttaccaa aacactcccc tcttggtgca gtttgttatt gttttttatg gcctacctct
    22861 tatcagtgac catactatca tgattccaat ttattggaca gctgttctct gcatgggact
    22921 ctatcacggc gcttatatcg ctgaggttat tcgttcaggg attcagtcta ttcctagtgg
    22981 tcagatggag gccgccttgt cgcaaggttt tacctatatc agtgccatgc gcttgattat
    23041 cttgcctcag accttccgca tcattctccc tccattgacc aaccaaattg ttaacctcat
    23101 caagaacacc tctactgtag ctatcatctc tggagtagac ttgatgtttg tgactaagtc
    23161 ttggtcggct ctcaacggaa actatatccc agccttttta ggtgctgctc ttctctactt
    23221 tgctctatgc ttccctgttg cccagtttgg tcgcaagatg gagcaagcca acaaaaaagc
    23281 ctattcactt taggaggtta ctatggaatc tattttagaa gttttaaccc cagataacct
    23341 agtctttatc tttaaaggat ttggcttgac cctctatatt tctctgattg ccatcatcct
    23401 ctctactatc atcggtacgg tgctagctgt catgagaaat ggcaaaaatc ctgtcttacg
    23461 cattatttcc agtatttata tcgagtttgt gcgcaacgtt cccaaccttc tctggatttt
    23521 tactatcttt ttggtgttca aaatgaaatc cacaccagca ggtattacag cctttactct
    23581 ctttacatca gcagccttgg ctgagattat tcgaggcggt ctcaataccg tagacaaggg
    23641 acagtacgaa gcaggaatgt cacaaggctt cacctcagcc caaatcctct actacatcat
    23701 tctcccacaa gccatccgca aaatgctacc agccatcatt tctcagtttg ttaccgtgat
    23761 taaggatacc agtctcctct actctgttat cgccctacaa gaactctttg gagccagcca
    23821 aattctcatg ggccgttatt tcgaaccaga gcaggtcttc agtctttgca tcctgattgc
    23881 cctcatctac ttcagcttta acctagcaat ttctagcctg tctcatatgc tagccaaacg
    23941 ttggcaacaa gctgcagaat aatactcttc gaaaatctct tcaaaccacg tcagcttcac
    24001 cttgccgtat atatgttact gacttcgtca gttctatcca caacctcaaa acggtgtttt
    24061 gagcagcctg cggctagctt cctagtttgc tctttgattt tcattaagta taaacagcca
    24121 ctctccagtt caattggaga gttttttgat gagagtagat atttatagac ctcaatttga
    24181 caccatacat tttctatgat aaaatgtaac aaattcattt acaacaaatc gaaatctaaa
    24241 tgaaatggaa ttttctatga aatcagtcaa aggactactc tttatcatag ctagttttat
    24301 cttgactctt ttgacttgga tgaacacttc tccccaattc atgattccag gactagcttt
    24361 aacaagccta tctctgactt ttatcctagc cactcgtctc ccactactag aaagctggtt
    24421 tcacggtttg gagaaggtct acaccgtcca caaattcaca gcctttctct ccatcatcct
    24481 actaatcttt cataacttta gtatgggcgg tttgtggggc tctcgcttag ctgctcagtt
    24541 tggtaatctt gccatctata tctttgccag catcatcctt gtcgcctatt taggcaaata
    24601 catccaatac gaagcttggc gatggattca ccgcctggtt tacctagcct atattttagg
    24661 actctttcac atctacatga taatgggcaa tcgtctcctt acatttaatc ttctaagttt
    24721 tcttgttggt agctatgccc ttttaggctt actagctggt ttttatatca tttttctata
    24781 tcaaaagatt tccttcccct atctaggaaa aattacccat ctcaaacgct taaatcacga
    24841 tactagagaa attcaaatcc atcttagcag acctttcaac tatcaatcag gacaatttgc
    24901 ctttctaaag attttccaag aaggctttga aagtgctccg catccctttt ctatctcagg
    24961 aggtcatggt caaactcttt actttactgt taaaaattca ggcgaccata ccaataatat
    25021 ctatgataat cttcaagccg gcagcaaagt aaccctagac agagcttacg gacacatgat
    25081 catagaagaa ggacgagaaa atcaggtttg gattgctgga ggtattggga tcaccccctt
    25141 catctcttac atccgtgaac atcctatttt agataaacag gttcacttct actatagctt
    25201 ccgtggagat gaaaatgcag tctacctaga tttactccgt aactatgctc agaaaaatcc
    25261 taattttgaa ctccatctaa tcgacagtac gaaagacggc tatcttaatt ttgaacaaaa
    25321 agaagtgccc gaacatgcaa ccgtctatat gtgtggtcct atttctatga tgaaggcact
    25381 tgccaaacag attaagaaac aaaatccaaa aacagagctt atttacgaag gattcaagtt
    25441 caaataagta atcatactct cctgggattt cccaggagag ttttttatag ccaaatgaaa
    25501 taaaaccgga acgatggaat tttggcattc aaatcaattg ataagaatat tttatagtgg
    25561 agtgaaatag gatacgaaca aattgattag gaaaatcaaa tgaatttata gaaatctttt
    25621 agcagttatg ttgtcctatt ctagtttcaa aacgctatag aagcagcatt gtgctagtct
    25681 agattcagtt tactatacta aaacgagtag cttgaaatca aaaaacccac cctcacaggc
    25741 aggttttatc tgtattattc agctagatta tgctttacct tctgaaccga atacgtcgat
    25801 acgttcttca accgatgctt ggatagcttt tacaccgtca gccaagaatt tacgtgggtc
    25861 gaagagtttt ttcttgtcgt attctgcttc gtttgcttcg tagtcacgag caaatttacg
    25921 agttgcgtta gcgaatgcga tttggcattc tgtgttaacg ttaactttgg caacaccaag
    25981 tttgatagct gcttggattt gctcatcagg aatacctgat ccaccgtgca atacgattgg
    26041 gaatcctgga agagcttctg tcaatttttg caagtggtca aggtcaagac cttcccagtt
    26101 tactgggtaa gggccgtgga tgttaccgat accagctgcc aagaagtcga taccagtttc
    26161 aaccattgct ttagcgtctt cgattggagc caattcacct ttaccgatga ttccgtcttc
    26221 ttcaccaccg atagtaccaa cttcagcttc tactgagata cctttagcgt gtgctttttc
    26281 aacaacttct ttagccaatt taaggttttc ttcaactgga aggtgtgaac cgtcaaacat
    26341 gattgaagta taaccaactt cgatacactc aagtgcatct tcgtagtgac cgtggtcaag
    26401 gtggatagct actggtacag tgatacccat tgattcaaca aggttagcga tcaagttgcg
    26461 agcaactttg taaccaccca tgtatttagc agcacccatt gaagtttgga tcaaaactgg
    26521 agctttttta gcttctgctg cgcgcaagat agcttgagtc cactcaaggt tgtttgtgtt
    26581 aaatccacca actgcataac cgttgtcacg ggctgcttgg acaaattttt ctgctgaaac
    26641 gattgccatt ttatcaggcc tcctgtatat ttttatgggt catcccattt acattgttca
    26701 ttttatcact ttttgccaaa aaaatctagt ttttcccgca gtttcgattg attttcttct
    26761 aactccatct atgtaaaccc tttctctccc tagtcttgga cgacttttgg aaaatctata
    26821 aagaaggtta aacgattctc ctccatctcg aaacgataag ctaatttttc atgttctaat
    26881 agactcttaa ccacaaagag ccccatacca gaccccttga ccttgcgact ggcattgtca
    26941 gaaaaagact gggctagttt ttcttgttcc tctgagctac agctattttc gataaaaagt
    27001 tctccttctc tttctccaat tcgaactaag ccacctggaa cagagtgctt aatggcattg
    27061 ctgatgagat tagaaagaat caacttcata actgatgggt ttagataagc ctgctgatgg
    27121 gtcaaactat tgtctatctg gagctctctt tccttggcta gcaaggcata atctttgacc
    27181 agattttgcg tcatctggag gaggtcaatt gtttccctat catctcgcaa ttcctgcaca
    27241 gaagagaggg aaagtatctg cagaacatgg tgattgagtt catccacaat ccccaaggca
    27301 actcccagat actggtctct atccttataa cgaccgatat tctctctcat attttcgatt
    27361 aggattttca aactagccag cggtgttttc aattcatgag aagctcctcg taggaattcg
    27421 accttcatct tctccagctg gagaatggct tcattctttt catgcaagtc cgcaataaca
    27481 gtcaagagat gctggtagag gctattgatt tgttccttga gattacctat ctcatcctta
    27541 gaatccacgc gcaatcgcac ttgggaatcc aggtccatca tccgacgggt cacccgcttg
    27601 atttccaaga tcggtgcaac aatagtccga gcgtagatgt aggccaccaa gagggaaatc
    27661 agaaaggagg ccagcaaggt atagggaaga aactggagac tgatttgctc cgcttccttt
    27721 tgtaaatcca tggaagctag aaactggaga atcatagtag caccgtcttg cgttttcacc
    27781 tcgcgctcct caataaagag agaggttgtc tggcggtctg tgtccagagg aagactgtcc
    27841 ttgacttcta acttgtcctc ggtcatctca cctttgacgg tccccttgat atcactagtc
    27901 tgggaataca agtctaacac ttgctcgata ctctgcctat ctttcccttc tagggactgg
    27961 gcaatggctg ttgccttttg accaatggtt tcctgacgat gactcagata agtcgaagga
    28021 aaaagaaaat aaatagctaa atgaaggcag ataaccagaa cactaaatat cgagaaggta
    28081 tagataaata tctttgcaaa taaacctgtt cgtttcattt tcgctccaat ttataaccaa
    28141 cattgcgcac agtgaggata caatccaagt ctagcttttt ccgcaattcc ttgatataaa
    28201 catcaataac acggtcaaag ggaacctcat ctgtcgcttt ccagacggca tcgataatct
    28261 gagatcgagt caaggcccgg ccttcatttt tcactagata gtccagaatt tccaactctt
    28321 tggcattgat aggcacttct tgacctgcga tgcttgcact gtagctttca aagtccacct
    28381 tggtatcctt gtaagaaaag attcgtcctg tatcgtagta gcgcttgaaa atcgcgtcca
    28441 ccctcacttt taaaagggag agggagaaag gtttttccag atagccatct gccaaagagg
    28501 caaaggcact catcttgtat tcctcatctt gaaaagctgt caacatcaag acaggaacct
    28561 gactggtttt acgaatctca gctaggactt ctaagccgtt gagcttgggc atctggatat
    28621 ccagtaaaac cagggccacc tcatagctag aaaattgctc cagagcttcc tgaccgtccg
    28681 ctgcctcaat agtttcatag ccacaatccg tcaaataatc actgaccccc tcacggatca
    28741 tctcttcatc ttctacaatt aaaattttca tactttaact gctctctatt ttttattttt
    28801 cttagaataa atacctactc tattttctat tatagtctct tgctggcctt ttgtctgcaa
    28861 gcaactgacc actagataaa acgttgtgaa attcctttct cataaattcc ataactttag
    28921 tatattatat ttaagcacta aagtacaaag aaagcaactg aaagcaatga ttttcaccac
    28981 tgctttcgga tttattttga attgttaaat agccattcct atccactatt cttgaataga
    29041 aacacaagat gcaatcttta ttctagactc attttttcaa atttattcac catccagcaa
    29101 gagctctttt ggttgttttc taaggagatt gcttgaagca agcgccataa cgagaaccac
    29161 tagaaccaag gcaaggacaa aaatgatgat aaagtctgat gtctgaatgg aaatgtctag
    29221 gctcgacaag gtcttgctaa agccatctac ttctgcacca ccaccaaggt tagaggcttg
    29281 agccgcctta ctagcctgtt tggcaacacc tgaagtcaca ttggcaagga cagtgtttcc
    29341 aattgcacgg gcagtgtaat tagctaggaa gtaagcagaa actagagcag ggatagcaat
    29401 caagatagat tcggtgatga attgacccaa gatacttgcc tgcttgaggc cgatagagag
    29461 gagaattccc acttccttgc gacgggcgtt gatccaaagg ctgagcaaga gggcaaggag
    29521 gagaactgag aagctcaagc taccccagaa gaggaggttg gccatcttgt acataccaga
    29581 gatagattgc tcaagagctg ggtagttaga ggagctcttg acgagtgtgt agctcttcca
    29641 gttgatacca ctgatgccat tcaactcttt cataacatca tccaagttct tgtctgctgt
    29701 tacaaagaag gttgcgtccc cataaatggc tgtgtcttct gtgtatccat aaagttttgc
    29761 agcagtgtga atgtctgtaa tagctgtgtt ttcgtaaagt tcttgtgagt aggttactgc
    29821 tgacttatta tgaccatcaa agagtccctt gattgtcact tcaactgttt ccttggctcc
    29881 tttttcatta tctgcatcgt agatattaga gtccagttta accttgtccc ctactttcca
    29941 gccgtgtttg gctgccaagt ccttgtgcaa gaggatttta tccttgtcgt cgttggttaa
    30001 gtgctctcct tcgactagtt tataagaacc agagacaaac ttgtcttctt tagaggagtc
    30061 attgacacct gtaatcatca agctacttcc aaaacgcttg gcacgatcag cagtgagatt
    30121 cttcttggtt tctggcgttt caatcaggtc atatccagtc aaatctccga tagcgttgat
    30181 acgtttgaca taagactcga tggccttgtt ttcggtgatt tttttgatgt cttcaccctt
    30241 gatattccca gcaccacgag gcgttccttg gttgacgcga cgattgattt gcatggagaa
    30301 gctattagtg atatttttaa aggtctcctg agaagccttg gcagtagctc ccttgattga
    30361 caagccgacc aaactcaagc tcgccatgag gagaataatc aggaagatga caatcgattt
    30421 gaaaaacttc cttgtaacat aggcaaatgc gttgtgtaac atagattccc tttctagatt
    30481 ttgttttaat cattctatca aaataagctc aaattattta ctagtattgc gcgtttcagt
    30541 cagtttctta tcctttaatt caagtgtaat atctgacgct tgtgccactt ctttactgtg
    30601 agttacgaca atcacacatt tacctgtttt ctgggcaagt gatttgagta gttcgacaat
    30661 atctccagca gttttaggat ccagatttcc tgttggctca tcagctagaa taactggagc
    30721 ttctgagacc aaactgcgag caatggcaac acgttgctgt tgaccacctg ataactggag
    30781 aacattccgc ttgatctggc tttcatccaa accaagctca agaagtgtat ccttgcttgc
    30841 ctttttgttg accaagcgga tattttccag cggagaaaga taatctatca agttataatt
    30901 ttgaaagacc agggaaatat ggtgcatgcg atggtaagaa tagcccttct tacgaatatc
    30961 ctctccttga aaaaggatag aaccttcaac aggactatct agaccagcaa gtagggacaa
    31021 gagtgtggat tttcctgctc ctgactcccc aataatactg taaaattttc caggttcaaa
    31081 attataatcg atctgatata ggactgcttc agcagtattc ttataacggt aggtaacatc
    31141 ttgtaattgt aataaagtca tgatttctcc ttcttaacta atagatgata aaatttcttt
    31201 cggtgatttt ctaaataaga ataggaaaca aagggctaca gataagcaac taagcagaac
    31261 tagaaaaaca taggattctg caaaagataa gatgctagtt gataaactgc ttgctttggc
    31321 tagtgtatct tgtaagcttg cctgatctcc acttgctagt agagtttgga gtagataact
    31381 tgtgattccg tttcctgcaa caaatgctgg aagcaaagct ccaagagata ccaaaactac
    31441 ctctaaacag aattgtagga agatcgagct cttgcctttt ccaagtgcaa gtaaaatccc
    31501 cacttcatag acccgttctc tcaaccagag agacaaaacc agaattaagg ctccagctcc
    31561 tgctatcaac atcccataaa ggaagatggt caagaaggtt tggaaagttg caactgagtc
    31621 tttgatttgt tcaaaagcct tgttttcctt ttcgacttgg tagccttgat tttccaaggc
    31681 caagttttct acctgcttca tgagtccgtc catttcctta ggattttcta catagaagcg
    31741 tgctgcactg acttgagctt cactattgcc caaaagggtt tggctacttt catagtctgt
    31801 aaagacttga ttttcactga agtcagaaga caagcctgtg aatttctctt gttttttacc
    31861 agaaaagatg ccgataatct caaactctac tgtttgtcct tttccagatt cagactgacc
    31921 agcatccaag ccaatcttgt catgaagtga aagaccgttc ttcttagcca attcttcgtg
    31981 gataaggatt ttcttggaat cccctttttg aaggtgtcgc ccttctttta gattgaaagc
    32041 cgaactggta aaggttacat ccttggatga atcctcaaga gccgttaagc taaccaagtt
    32101 attgtctgca gctgataaat catcacgctc cacgctctgc tcaccagtca ctgcttcctt
    32161 gtcttttagt tttgcgaccg tctcaagttc aggagagaca ttttccagcc ccttaatctt
    32221 gcttacagat gctaggtctg acaacttgaa tgtctgacca ttctctatct tcttaataga
    32281 aaaagatgta ttgagtgatt tataaagatt gctttctact gttttgttgg acttcatcag
    32341 agtcaaacag gctgaaattc cggccaataa gaccaataaa atcagaaata aaataaaact
    32401 tctcagtcgc tttctgctga cataagccca agatctttgg attggattca tttgtcacct
    32461 ccatatttgt aagactatta taaaacccaa atatgaaatg tttatgaaat acgaaaaaaa
    32521 atatcgagta ggggataatc tctagcccct ctcacaccac cgtacgtgcc gttcggcata
    32581 cggcggttca actaactttt aacgcatgtc gttcaaggta ataatccaaa cacgaaacct
    32641 gttcacgtt
//
LOCUS       DAVBCN010000016        32444 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVBCN010000016 DAVBCN010000000
VERSION     DAVBCN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32444
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.016"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..98
                     /locus_tag="VQ497_000686"
     CDS             <1..98
                     /locus_tag="VQ497_000686"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2517031.1"
                     /translation="FRRKLLPLPPYSPEYNPIEKTWALLSCSCFN"
     gene            254..592
                     /locus_tag="VQ497_000687"
     CDS             254..592
                     /locus_tag="VQ497_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET2517032.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVEEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQETLDKHHQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
     gene            782..2218
                     /locus_tag="VQ497_000688"
     CDS             782..2218
                     /locus_tag="VQ497_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET2517033.1"
                     /translation="MTTFKDGFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITLGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVI
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGENREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPATCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTAQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            2270..2509
                     /locus_tag="VQ497_000689"
     CDS             2270..2509
                     /locus_tag="VQ497_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HET2517034.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            2639..2953
                     /locus_tag="VQ497_000690"
     CDS             2639..2953
                     /locus_tag="VQ497_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HET2517035.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            3071..5050
                     /locus_tag="VQ497_000691"
     CDS             3071..5050
                     /locus_tag="VQ497_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET2517036.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFAQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDAIERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQKNQKNISEEVLKKSLFKTFQNLGLDDMYNFSSDFQLIEGLIT
                     HLMTLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAYVS
                     LHFLAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQE
                     KLKGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINK
                     FENNSFRQYFSKENFLICTESDKVNLLEKMVESLSVGESNEFEQSLLYGIKQREELSS
                     VVFSEKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSYVQLVFLLSPSIYGNEGLAT
                     VTKKIVSMTENDELQNQLISCNNFEDFINIFEKIK"
     gene            5060..5374
                     /locus_tag="VQ497_000692"
     CDS             5060..5374
                     /locus_tag="VQ497_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HET2517037.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            5405..5911
                     /locus_tag="VQ497_000693"
     CDS             5405..5911
                     /locus_tag="VQ497_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517038.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFISPSPIIIFPIMLIVFSILATIEQFKLYGNRKEKLGITLMFVRIQMLISIGLLV
                     LTWTSWFENLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAYQQFVQ
                     LEKNSLSL"
     gene            5991..7337
                     /gene="celB"
                     /locus_tag="VQ497_000694"
     CDS             5991..7337
                     /gene="celB"
                     /locus_tag="VQ497_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HET2517039.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNSLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            7717..7893
                     /locus_tag="VQ497_000695"
     CDS             7717..7893
                     /locus_tag="VQ497_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517040.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALSLGMT"
     gene            8295..10508
                     /locus_tag="VQ497_000696"
     CDS             8295..10508
                     /locus_tag="VQ497_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HET2517041.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEDEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(10730..11206)
                     /locus_tag="VQ497_000697"
     CDS             complement(10730..11206)
                     /locus_tag="VQ497_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HET2517042.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDHKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTNPKQIEEAIQTLL"
     gene            11429..14632
                     /locus_tag="VQ497_000698"
     CDS             11429..14632
                     /locus_tag="VQ497_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HET2517043.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSTWVDQKNSSADASTRVIEAKDG
                     AITISSHEKLRAALHRMVPIEAKKKYKLRFKIKTDHKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQPSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDIASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKSGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            15012..15397
                     /gene="tnpA"
                     /locus_tag="VQ497_000699"
                     /pseudo
     CDS             15012..15397
                     /gene="tnpA"
                     /locus_tag="VQ497_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(15776..16405)
                     /locus_tag="VQ497_000700"
     CDS             complement(15776..16405)
                     /locus_tag="VQ497_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HET2517044.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSALSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
     gene            complement(16415..17416)
                     /locus_tag="VQ497_000701"
     CDS             complement(16415..17416)
                     /locus_tag="VQ497_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HET2517045.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(17447..18088)
                     /locus_tag="VQ497_000702"
     CDS             complement(17447..18088)
                     /locus_tag="VQ497_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HET2517046.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(18107..18922)
                     /locus_tag="VQ497_000703"
     CDS             complement(18107..18922)
                     /locus_tag="VQ497_000703"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HET2517047.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            19193..19627
                     /locus_tag="VQ497_000704"
     CDS             19193..19627
                     /locus_tag="VQ497_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2517048.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            19639..20829
                     /locus_tag="VQ497_000705"
     CDS             19639..20829
                     /locus_tag="VQ497_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HET2517049.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSAQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            20840..21331
                     /locus_tag="VQ497_000706"
     CDS             20840..21331
                     /locus_tag="VQ497_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET2517050.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            21346..22125
                     /locus_tag="VQ497_000707"
     CDS             21346..22125
                     /locus_tag="VQ497_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2517051.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            22112..22930
                     /locus_tag="VQ497_000708"
     CDS             22112..22930
                     /locus_tag="VQ497_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2517052.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPTKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            22930..23223
                     /locus_tag="VQ497_000709"
     CDS             22930..23223
                     /locus_tag="VQ497_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET2517053.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            23245..25146
                     /locus_tag="VQ497_000710"
     CDS             23245..25146
                     /locus_tag="VQ497_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HET2517054.1"
                     /translation="MKEERRQFFERIDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYHFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHRRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            25206..26207
                     /locus_tag="VQ497_000711"
     CDS             25206..26207
                     /locus_tag="VQ497_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2517055.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKKLNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(26317..26652)
                     /locus_tag="VQ497_000712"
     CDS             complement(26317..26652)
                     /locus_tag="VQ497_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517056.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTTPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(26663..26905)
                     /locus_tag="VQ497_000713"
     CDS             complement(26663..26905)
                     /locus_tag="VQ497_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517057.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(26916..27458)
                     /locus_tag="VQ497_000714"
     CDS             complement(26916..27458)
                     /locus_tag="VQ497_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HET2517058.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADSIGNQVFIILFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLSILIDEGQDYFHSLLTIRNGVSSVLGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            complement(27474..27668)
                     /locus_tag="VQ497_000715"
     CDS             complement(27474..27668)
                     /locus_tag="VQ497_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2517059.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            27834..28784
                     /gene="rsmH"
                     /locus_tag="VQ497_000716"
     CDS             27834..28784
                     /gene="rsmH"
                     /locus_tag="VQ497_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET2517060.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIHQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            28796..29113
                     /gene="ftsL"
                     /locus_tag="VQ497_000717"
     CDS             28796..29113
                     /gene="ftsL"
                     /locus_tag="VQ497_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HET2517061.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            29117..31369
                     /gene="pbpX"
                     /locus_tag="VQ497_000718"
     CDS             29117..31369
                     /gene="pbpX"
                     /locus_tag="VQ497_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HET2517062.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKATAETFAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            31371..32351
                     /locus_tag="VQ497_000719"
     CDS             31371..32351
                     /locus_tag="VQ497_000719"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET2517063.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGIIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
BASE COUNT        10218 a         5344 c         6972 g         9910 t
ORIGIN      
        1 agtttaggcg taaactttta cctcttcctc cctactcacc tgagtacaat cctattgaga
       61 aaacatgggc tcttttgtcc tgctcttgtt tcaattgact ataaatcgct gtaagaaaaa
      121 atcattttat tggtataaag aagtgattga aagtaatggc gaaacgttag ataattagtt
      181 cataaaaaac aagtttagct cctcatctaa acttgttttt ttaaaattga aggatgattt
      241 tggaggatat aagatgacat tgactattca tgtatattat aaaggtgatg gagatagtgc
      301 aataaagttt gcaagagaaa tgcttgatag tggccttgtt gaagaaatta gaaatcaaaa
      361 aggtaacttg aaatacgaat attttttacc aattgaaaaa gagggaacta ttctccttat
      421 tgatcagtgg ataaatcaag aaactttaga taagcatcat caatcaaaga caatgcagaa
      481 aatcttagat ttaagaaaaa aatatcactt gcagatgcaa gttgaacgtt atatagaaga
      541 tgattctggg atgccagaaa gcgataagtc gtttatagac acagggaatt agaattattg
      601 tgagagtttt tgtagtttga aagtattgaa aatcgtaaag atatcattac gagaatacaa
      661 gaagatattg ttctatttcc attaaaaatt acttctttcc ttaacaataa ggaaaaagaa
      721 tgcgttttca tcttttggtt tgaggtttat aataagagta tcaaagtcag gaggaaaaaa
      781 gatgactact tttaaagatg gatttttatg gggtggtgct gttgctgctc atcaacttga
      841 aggtggatgg caagaaggtg gcaagggaat tagtgttgct gatgttatga ctgctggtcg
      901 tcatggagta gctcgtgaaa ttactttggg agttttagag ggtaaatatt atccaaatca
      961 tgaggcgata gatttttatc atcgttataa agaagacata gcactttttg ctgaaatggg
     1021 attcaagtgc ttccgtacct ctattgcatg gacacgtatc tttccaaaag gtgatgagtt
     1081 agagccgaat gaagaaggat tacagtttta tgataatctt tttgatgaat gcttaaagaa
     1141 tggtattgaa cctgttatca ctctatctca ttttgaaatg ccttatcact tagtgaccga
     1201 atatggtggt tggaaaaata ggaaattgat tgatttcttt gctcgttttg cagaagtcat
     1261 atttaagcgt tacaaagata aggttaaata ttggatgact ttcaatgaaa tcaataatca
     1321 agcgaattat caggaagatt ttgcaccatt tactaactca ggtattgtat atgaggaagg
     1381 tgagaataga gaggcaatta tgtatcaagc agcacattac gaattagttg cttctgcacg
     1441 agctgtaaaa attggtcatg agattaatcc agattttcaa ataggttgta tgattgcgat
     1501 gtgtccaatt tatccagcta cttgcaatcc taaggatatc ttaatggcaa tgaaagctat
     1561 gcaaaagcgt tattattttg ctgatgtgca tgttttaggt aaatatcctg agcatatttt
     1621 caagtattgg gaacgaaaag gtatttcagt tgattttact gcccaggata aagaagattt
     1681 acttggtggg actgtagatt acattggttt cagttactat atgtcctttg ctatcgactc
     1741 tcatcgtgaa aataatcctt attttgatta tcttgaaaca gaagatttag tgaaaaataa
     1801 ttatgttaag gcttctgaat gggagtggca aattgatcca gaaggtttgc gttatgcgtt
     1861 aaattggttt acagaccact atcacttacc actctttatt gttgaaaatg gttttggagc
     1921 tatagatcaa gttgcagcag atggtatggt acatgatgat tatagaattg aatatctagg
     1981 tgcccatatt cgtgaaatga aaaaggctgt agttgaagat ggtgttgatt taatgggtta
     2041 tactccatgg ggatgtattg atttggtttc agctggtacc ggtgaaatgc ggaaacgtta
     2101 tggctttatt tatgtagata aagatgataa tgggaaggga agttataatc gttccccgaa
     2161 aaaatctttt ggctggtata aggaagttat ttcatctaac ggtgaatcag tagaatagta
     2221 taaacaaact gagattattc tcagtttgtt tagcatattg aggtgacata tgattggaag
     2281 tattgcagca attttaacaa catttgcatt tttaccacaa gtttttcgtg tagttaagac
     2341 aaaggacaca ggatctattg ctctaggtat gtatgttatg caagttattg gtattgcatt
     2401 gtggcttgct catggtattc gtataggaga cttgcctttg attttagcaa atagtgtttc
     2461 atttctactc tcaggaataa ttttatttta taaattgaag tataaataat ttttttccat
     2521 atcatttagg aaaatgaggg aattgtcatt ggaaacgctt tattttataa taaaaataga
     2581 taacaaataa gccaacttag cacagctttg attggattta agaaaaggag aaaatattat
     2641 ggcaaaagct ttaattattt gtgcagcagg aatgtcttca tcacttatgg cacagaaaac
     2701 aacagagttt ctaaaaggaa aagggaaaga tattgtagtt gatgctatta gtgctactga
     2761 aggagggaaa gctatcgcag cagcagcttt tgatttgtat cttgtcagcc ctcaaacaaa
     2821 aatgtacttt aagcaatttg aagaggctgg ttctaaagca ggaaaaccag ttgtacaaat
     2881 acctcctcaa gcctatattc ctattccaat gggaattgaa aaaatggcta atttgattct
     2941 tgagaatatt tgatgaacac atgaaatgtc aataaatatt taagttaaga taaattgttt
     3001 tgagtttgat ataaattgat ttaagtgtat aatatgcttc aatatatgaa aacatacgtt
     3061 ttaggaggga atgaatgtgt taaacattaa agagaaagat atccttcaat ttttaataaa
     3121 aaataaggaa cgttttgtta caagcaaaga actggcagaa tatttatcct gttcagatag
     3181 aactgtccgt aatgtactga aattaattga gaagacaatg attattcaag gagtccggtt
     3241 gatttcaaaa caaggtcaag ggtatcaaat attttttgag aatcaaggcg cttaccagga
     3301 gtttagacaa acttatgaac tggaagaaga ctatacgaaa actgcggttt caaaaggtga
     3361 tgatcgttta gtatttatct taaataaatt attatttgag caagttccag ttctttttga
     3421 tgatttagct gatgaacttt acgttagtcg ttcaacatta tctcatgatt tcagaaagat
     3481 acgagtgatg ttatcagagt ataatttgtc cattgagagt agggcgaata aaggagttta
     3541 tgtctcaggc gaggaacgtg ataaacgacg ttttatcata aattatttcc tagagaatca
     3601 attttttaaa acaatacatt gctatgtaaa atttaacttt tttgctcaaa ctgttccatt
     3661 agaagaattt gctagaattg ttttggatga atgccaagag gccaacctaa aactatctga
     3721 ttttgtacta cagaatttag tagttcacat agcattgtct gtgattcgtt taaaatccgg
     3781 atttgaaata aaaaatatag actgtcagat gactgatgat gcaatagaga gaaaggttgc
     3841 tcaaagaatt ctttctaaag taagagaagt aacaaatcag gaatttccag ttcaggaaat
     3901 agactacatt actttgcatt tgttagctaa gagtcaacaa tgtcaaaaaa atcagaagaa
     3961 tatttctgaa gaagttttaa aaaaatcttt atttaaaaca tttcagaatt taggattaga
     4021 tgatatgtat aacttttctt cagatttcca actaattgaa ggattgataa ctcatctaat
     4081 gacactacaa gtacgtttag aaagccggat taccttaaat aaccctttgg tagatgaaat
     4141 taaacagaat tatagtgata tattctttat gactagagaa attcttgcaa atatggacaa
     4201 gttcttggag tggacaatat cagatgatga agtagcatat gtaagtcttc actttttagc
     4261 tgctatggaa cgtagcaaag agagtactaa gtttaatatt cttgctattt gtgcaacagg
     4321 atttggtgca gcccaaatgt taagaaatcg tttggaaacg gagtttggga agcgagtaga
     4381 agttgttgat gtaataggct attatgaatt gaatcaagaa aaacttaaag gaatagattt
     4441 tatagtgtca gctgttgatt tatcaaatct atattttcaa attccagttt ttaaagttag
     4501 tgtgtttctt aagagtgatg agatggaaat gattagaaaa gctatggatc agatgcaagt
     4561 ttctagttat gtacaatcat caaagattaa taaatttgaa aacaacagct ttagacagta
     4621 ttttagtaaa gaaaattttt taatatgtac tgaatcagac aaggtaaatt tacttgaaaa
     4681 gatggttgag agtttatctg ttggtgaatc aaatgagttt gaacaatctt tactttatgg
     4741 aattaaacag cgcgaagaat taagttcagt tgttttttct gaaaaaattg cagttccgca
     4801 tcctattcag ccctttggta cagagggaaa ggtttcggtt gcaatttgta aagattcgtt
     4861 actctgggat aatcaaagtt catatgtcca gctagtattt cttctatctc catcaatata
     4921 tgggaacgaa ggtttggcaa ctgtgactaa aaagatagtc tctatgacag agaatgatga
     4981 attgcaaaat cagctaatat cttgcaataa ctttgaagat tttattaaca tatttgagaa
     5041 gataaaatag gaggttcata tgaatcaaga ggaactgcag gttgcagcat ttgaaattat
     5101 tctgcattcg ggaaatgcaa ggtcagaaat acatgaagca tttgcaaaaa tgagagaagg
     5161 tagttttgat gatgcagaaa gtaaattaaa tcaatcaaat gagataattc ttgaagcgca
     5221 tcatgcacaa accaaactct tgcaagaata cgcaagtgga gtggaaatta aaattgaaat
     5281 tatcatggta catgctcaag accatttaat gaccactatg acattattag aagtagcaaa
     5341 ggaaatgtta gctctttata agaaagttaa ctaaatatgt gttgttatat aaggaggaaa
     5401 tgatatggat aaaaaagaac gacaaaaaat tgaacaacaa cgtcgagaga tggctctaac
     5461 taatactttt tttaatcgat atcttcttct acgttattca atagcactct tcttttttgg
     5521 gaatatttat tggcttttga atcagtttat tagtccctcg cctattatta ttttccccat
     5581 tatgctcata gtattttcta ttttagcaac tattgaacaa tttaaacttt atggaaatag
     5641 gaaagaaaag ttgggaataa cactaatgtt tgttagaata caaatgctta tttcaatagg
     5701 attacttgtt ttaacttgga caagttggtt tgagaatctt tttccgattt ttgaaaataa
     5761 ccaagtagca cgtttatttg tttttgtagt tcttctatta ggtttagttc tgagtttgtt
     5821 agacattaga agaattaaaa aaatttataa acgaacagat aaggcttatc aacaatttgt
     5881 ccaattggaa aagaactcac ttagtttata atcctgaact agtataattt gtatttgaaa
     5941 ctagtcttta aaaagtaaaa attattatgt tttattaagg aggtctattt atggatcaac
     6001 aaaacgggtt gtttggtttt cttgaaaacc atgttatggg accaatgggc aaacttgctc
     6061 agtttaaagt agtgcgtgct atcacggctg caggtatggc tgctgtacca tttactattg
     6121 taggatcaat gtttttggta ttcagtattt tgccacaagc tttctcattt tggccaattg
     6181 tggcagatat tttctctgct tcatttgata aattcacatc actttacatg gttgcaaact
     6241 atgcgactat gggttctcta tctctttatt tcgttctatc acttgcatat gaattgacaa
     6301 aaatttatgc agaggaggaa gaactcaata tgaattctct taatggtgcc ttgctcgcct
     6361 tgatggcttt tgtcatgaca gtaccgcaaa tcatttttga tggtggaatg atgaagactg
     6421 tgacaagtct aaaagaaggt gcagtaattg cagatggatg ggcaatggga aatggagtcg
     6481 cacgttttgg tacaacagga atttttaccg caatcattat ggcaattgtg actgttctta
     6541 tttatcgtat gtgtgttaaa cataattggg ttattaaaat gcctgaagct gttccagaag
     6601 gagtttctcg tggatttacc gctttggttc cgggatttgt tgttgcattt gttgttatct
     6661 ttatcaacgg tcttcttgta gcaatgggaa cagatatttt taaagtcatt gcaattccat
     6721 ttggttttgt atccaatctg actaattcgt ggattggttt aatgattatt tatctattga
     6781 ctcaactact ttggattgta ggtatccacg gtgcgaacat tgtttttgca tttgttagtc
     6841 caattgctct tgctaacatg gctgaaaatg ctgctggcgg gcacttcgct gttgcaggtg
     6901 aattttctaa tatgtttgta attgcaggtg gttctggtgc aactttagga ctatgtttat
     6961 atattgcttt tgcctctaaa tctgaacagc ttaaagcaat aggacgagca tctgtagttc
     7021 cagccttatt taatattaat gaaccattaa tttttggatt acctattatc tataatccag
     7081 ccttggctat accatttatt ttagcaccaa tggttactgc tactatttat tacgtagcga
     7141 attctctaaa ctttattaag ccaattatcg cacaggttcc atggccaact ccagtaggga
     7201 ttggagcttt cttagggaca gcagatcttc gagctgtatt agttgctcta gtatgtgcat
     7261 ttgcagcatt cctagtctat cttccattca tccgtgtata tgatcaaaaa ttggtgaaag
     7321 aagagcaagg tatctaataa gtaaaaaagt atactcgatg atagaatcca tatagtttaa
     7381 ataatgagcg ttaacattag cagtgaagag gtctttttaa gatattcttc tctgcttttt
     7441 catatttttt cgctatgtag agtatgaagc aaaatagaaa gtagttcgaa tagttgataa
     7501 acagggaatt gctgttattt aaaaaaaaca ataaagtaaa gtatgttatc gtagaatacg
     7561 atacaatagt aaaaaagcaa aaagaatgcg ttttatattt gggtttgagg cttataaaat
     7621 aggataagcg tactgaaaac ttagatatga caaacaaaat aaaattttta aaagctacag
     7681 agaaacatgg tataatgtat ctaaaggagg tagcatatgt ccttagatat agataaagaa
     7741 aagatgacaa tcatgggaat agcttttgaa aaccgatccg tttttaagag tgtttggtat
     7801 gctttaagta caaatatgat tgaaggatgg cgacctacgg ttagtgatgt tgaaaaatta
     7861 cgagatgaag ctctttctct ggggatgact taatgaagcg caactgttat gagtcttatg
     7921 actatgttga tccggataat ttatataccg atccgggttc ttcagttcta agaaataagc
     7981 aggaaggaaa aaagcacgcg agcttgaata tcgtatgatt gctagtaaga gtttgaaatt
     8041 atttatcaat cctatcttag ttaatagttt aaaagtagtt tgtttcacaa ctgcataagc
     8101 ttagaaaagt tgaaaagtga taactcttta ataaactctt ggtttgttaa aagatatttt
     8161 gtccactaca tttttttcaa ttgactatta tttgtcatag atataacaga tagaacattg
     8221 cattcagaat aatgaaaaac gcatcaaaaa tggtgcgttc tgtttttatg ctaccataaa
     8281 aggaggaaaa caaattgaaa atttttaagg gagagtttta tcgaatctct gtattaacag
     8341 acaagctagt aaggttagaa tactctcgaa ctggaaattt tgaggataga acgacacaac
     8401 ttatctataa tagagatttt ggccaagttt cgttagatta tatcgagaca tcaaatgtac
     8461 tagatattat gacggactat tttcatctgc actttaataa aggagaattt aacgccgaaa
     8521 atttatttat agaattaaaa ggaaattttg ccgtatatgg tagtcgctgg tattttggtg
     8581 aatctattga aacgttaaaa ggaacagctc ggactctgga taaggcagat ggagcaatct
     8641 cgttagaaga tggaattatt agccgaaatg gtatagcctt attggatgat tctcaaggat
     8701 ttatttggga tgaacaatct ggttatattg agagagaaaa tcaaattgac ctgtatttct
     8761 ttgcctatgg gcatgattat agaggagcaa tcagagactt ttaccatttg actggttcaa
     8821 cacccttgtt gccaagatat gctttaggca attggtggag tagatattgg ccttatacgt
     8881 cggatgaata cttggattta atagacagat ttgaaacaga gaaaattcca ttatctatcg
     8941 gtgtgttaga tatggattgg catataactg acattccagc tcgttttgga agtggctgga
     9001 caggatatag ttggaataga aacttaatac caaatccaga acagttattg caacaacttc
     9061 atgatagaaa gttaaaactc tccttaaatg tccatcctgc tgatgggata cgggcttatg
     9121 aagaagctta tcctcaaatc gcaaaacggt tggggttaaa tgtagaacta gaagaacctg
     9181 ctatttttga tttttttaat ccctctttta gggaagccta ctttaaagat gttcattatg
     9241 agctagaaaa gcagggagta gatttttggt ggattgactg gcaacaaggg acacaaggta
     9301 tgctagatcc actttggctt ttaaaccatt atcactatca ggatagttgt aaaaatgcag
     9361 aaggtggttt gattttatca agatatgcag gtcctggtag tcaccgctac cctgttggtt
     9421 tttcagggga cactattatt agttggaatt ccttaagatt tcaaccctat tttacagcga
     9481 cagcatctaa tatcggttat agttggtgga gtcatgatat cggtggacat atgttggggg
     9541 attatgacga agagctacaa actagatggc tacagtttgg tgtttttagt ccgattactc
     9601 gattacatag ttctagaagt ccttttaata gtaaagaacc ttggttcttt tcagaaacaa
     9661 catctaagat tatgaagaaa taccttcgtt tgagacatca gatgattccc tatctatata
     9721 ccatgaatgt aaagacacat gaggaaggtg ccccattaat cagtccaatc tattatttct
     9781 atccagagaa tgatgagagc tataatgttc caaaccaata cttttttgga acagaactga
     9841 tggtggctcc cattgtagaa aagatggatt tgacattcca atctgcaaaa gtagatgtat
     9901 ggttccctga agatgaatgg tatgacttct tttcagagaa aaaatacaca ggtggtgtga
     9961 agttaagtgt ttatagggac atctcgacta cgcctgtgtt tgcaaaaagt ggtgcaatca
    10021 ttcccttggt tggttctgag ataggtatgg gtgttgattt acctgaggtt gtagattggt
    10081 atgtattccc agggaaacaa cattcttttg aaatgcttga agatcaaaat ggtcaaagat
    10141 ataaaacaag attatcaatc gattgggaaa tgggaatggt agagttagca ttacaaggag
    10201 attctagtat cgttccaagc aatagaaaac atagaattca ttttaaagga acgaatgtgt
    10261 ctataattga attgccaaat aagaatgata cagctaaatt tgaatggaaa gataataaaa
    10321 ggacatcttt aaatgacgaa gtttttagac tactaaagac agcttctctt ccatatgaat
    10381 taaaagatag attgttaaat caattcatca atgcgaaaaa ctctcatgac ttaatgaata
    10441 tcttgcatca tcaggataag gaattgagag ggcgtttgtt ggaaatgata tttactagcc
    10501 aaaactaatt gaaataatta gaaagtcatt tgtttagtgt atatgaagat tctgtatggt
    10561 attcgttatc actggtcaca ttgaaaatgt gaaataagca cctacaacat agactttaca
    10621 acacttcatg ttttatatca tggggtgttt ttctatttaa gtcaaagccc atcaaaaaat
    10681 ctcactattc atcaggttaa aggaaaccta atcatagtga gattgtgaat tatagtagag
    10741 tttgtatcgc ctcttcaatt tgttttgggt ttgtttttga agaaaagcgt tcaaagactt
    10801 gcccatcacg accaatgaga aacttagcga aattccattc gattcgtttt cctagtgggc
    10861 cagatttatg gtcttttaac cagacataga gagggtctgc ttccttaccg ttgaccttaa
    10921 tcttggcaaa acgtgggaag gtggtttgaa aatgtaggct acagaaggcg ttgatttcct
    10981 ctgcgctgcc gggtgcttgt cccataaact gattgcaagg gaaatccaat atttcaaagc
    11041 cctgatcttg atagcgttca tagagttctt gaagtccctg gtactggggc gttaaaccac
    11101 atccagtagc agtgttgaca atcaagagaa ctttaccacg atagctatcc aagggagttg
    11161 cttggttgtt ttggttcaaa acggaaaaat cataaagtga agtcatgagg gcctcttttc
    11221 tttttctaca ttgtaacagt ttttactttt ttcgtcgagt cggatggaag atattgttga
    11281 agaataagcc ttcaatatga tagaaaccgt ttacaaataa ctttaaaaat ggtatgattt
    11341 aattaaaccg gtttactttg ttgctataaa tctctaattt gagatttagt tcctattgat
    11401 tttacaatat gtttattgga gtgtatacat gcaaacaaaa acaaagaagc tcattgtgag
    11461 tttgtcttca cttgttttat caggattttt attaaaccat tatatgacag ttggagcgga
    11521 agaaacgact acgaatacca ttcagcaaag ccagaaggaa gttcagtatc agcaaaggga
    11581 tacaaaaaat ttagttgaaa atggtgattt tggtcagacg gaggacggaa gcagtccgtg
    11641 gacaggaagc aaagctcagg ggtggtcaac ttgggtagac cagaagaata gttctgcaga
    11701 tgcctcaact cgagtcattg aggctaagga tggggctatc actatctcaa gccatgagaa
    11761 attaagggca gcgcttcacc gtatggttcc tattgaagct aagaaaaagt ataaactgcg
    11821 tttcaagatt aaaacagatc ataaagtcgg gattgccaaa gttcgtatca ttgaggaaag
    11881 tggtaaggac aagcgattgt ggaattctgc aacgacgtca ggaacaaagg actggcagac
    11941 cattgaagca gactatagcc cgactttaga tgttgataaa atcaagctgg agttattcta
    12001 tgaaacagga actgggactg tttcctttaa ggatattgag ctggtagagg tagcagacca
    12061 gccttctgag gattctcaaa cagataaaca gcttgaggaa aagattgatt taccaattgg
    12121 aaaaaaacat gttttttctc ttgcggacta tacttataag gtagaaaatc ctgacattgc
    12181 ttcagtcaaa aatggaattt tagaacctct taaggaaggg acaaccaatg tcattgtcag
    12241 taaagatggc aaggaagtga aaaagattcc tttgaagatt ctagcctctg ttaaggatgc
    12301 atacacagac cgtttggatg actggaatgg catcatcgct gggaatcaat actatgattc
    12361 taaaaatgaa cagatggcca aattaaacca ggaattggaa ggaaaggtag ctgatagcct
    12421 atctagtatt tcaagtcagg cggaccgcac ctatttgtgg gaaaaatttt caaattataa
    12481 aatgtctgca aatctgactg ccacttatcg gaaattggag gagatggcca agcaagtgac
    12541 caatccttct tctcgttatt atcaagatga aactgtcgtt cgaacagtca gggattccat
    12601 ggaatggatg cataaacatg tctacaatag tgaaaagagc attgttggga actggtggga
    12661 ttatgaaatc ggtacacctc gtgccatcaa caataccttg tctctgatga aagaatactt
    12721 ctctgatgag gaaattaaaa aatatacaga tgtgattgaa aaatttgtac cagatcccga
    12781 acatttccga aagacgactg ataacccatt caaggctcta ggtggaaact tagttgatat
    12841 gggaagggta aaagtaatag ctggtttact gcgtaaggat gatcaagaaa tttcttctac
    12901 cattcgctcg attgagcaag tgttcaagtt ggtagaccaa ggtgaaggtt tttatcaaga
    12961 tggatcctat atcgaccaca ccaatgttgc ctatacgggt gcttatggga atgttttgat
    13021 tgatggcctg tctcaactgt tgccagtcat tcaaaagacc aagaatccaa tcgataaaga
    13081 taaaatgcaa accatgtacc actggattga taaatcgttt gctcctttgc tggtgaatgg
    13141 agagctgatg gatatgagtc gtggacgctc gatcagtcgt gcaaatagcg aggggcacgt
    13201 ggccgcagta gaagtactaa gagggattca ccgaatagcg gatatgtctg aaggagaaac
    13261 caaacaacgt ttgcagagtc ttgtgaagac cattgttcaa tcggatagtt attatgatgt
    13321 ctttaagaat ttgaagactt ataaggatat cagtttgatg caatccttgt taagtgatgc
    13381 aggagtcgca agtgttccaa gaacaagtta cctatctgcc tttaacaaga tggataaaac
    13441 agccatgtac aatgcagaga aagggtttgg atttggcttg tcactctttt ccagccgtac
    13501 cttgaattac gaacacatga acaaggaaaa taaaagtggt tggtatacga gtgatgggat
    13561 gttctatctt tacaatggcg atttgagtca ctatagcgat ggctactggc caacagttaa
    13621 tccatataag atgcctggta caacagagac ggatgctaag agagcggata gcgatacagg
    13681 taaagtttta ccgtctgctt tcgttggaac gagcaaacta gatgatgcca atgcgacagc
    13741 aaccatggat ttcaccaact ggaatcaaac attgactgct cataagagct ggtttatgct
    13801 aaaggataag attgcctttt taggaagcaa tatccaaaac acttcaacag atactgctgc
    13861 aactacaatt gaccagagaa aactggaatc aagtaatcca tataaagtct atgtcaatga
    13921 taaagaagcc tcccttacag aacaagaaaa ggattatcct gaaacccaaa gtgtcttttt
    13981 agaatcgtcc gattcgaaaa agaatattgg ttactttttc tttaagaaga gttcaatcag
    14041 tatgagtaag gctttgcaaa agggagcctg gaaggatatc aatgaaggac agtcagacaa
    14101 ggaagttgaa aatgaatttc ttactattag tcaggctcat aagcaaaatg gagattctta
    14161 tggctatatg ctcattccta acgtggatcg tgccaccttc aatcaaatga taaaagagtt
    14221 agaaagtagc ctcatcgaaa ataacgaaac ccttcagtct gtttatgatg ctaaacaagg
    14281 agtttggggc attgtgaaat atgatgattc tgtctctact atttccaacc aattccaagt
    14341 tttgaaacgt ggagtctata ccattcgaaa agaaggggat gaatataaga ttgcctacta
    14401 taatcctgaa acccaggaat cagctccaga tcaggaagtc tttaaaaagc tagagcaagc
    14461 agctcagcca caagtacaga attcaaaaga aaaggaaaaa tctgaagaag aaaagaacca
    14521 ttcggatcaa aagaatctcc ctcagacagg agaaggtcag tcaatcttgg caagtctagg
    14581 gttcttgcta cttggggcgt tttatttatt ccgtagagga aagaacaact aatttgttca
    14641 tcatctagat gaattacatg aaactgtgga aaatagaaaa aatccgaaga aggccaaata
    14701 tcttcggatt tttcttttaa aaagggataa gttgatactt atcctttttt acttcattat
    14761 gtgactatag cgttttgaaa ctagaatagg acgtcataac tgctaaaaga tttctataaa
    14821 ttcatttgat tttcctaatc aatttgttcg tatcctattt cactccacta tattagtccg
    14881 tctcgctccg tgaggtgcga gacaaaacaa ccacccgcta tgcgggtgcg cgtcgaaggc
    14941 tataccaaaa aactccaaac gcgatacaat aaaggtgttc aatccaattg taaagcgaaa
    15001 ggagaaaaaa tatggcacaa aagactcata gtttatcata cacaaagtgg atgtgttcta
    15061 tcatattgtg ttcaccccta agtatagacg aaaagtcatc tataatcaat atagaagtag
    15121 tttaggcgaa atatttcatc gcttgtgtgg ttataaaggt gttgaaatga tcgagggtca
    15181 cttaatgcca gaccatgtac acatgttagt cagtattcca ctaaggctaa gtgtttcgag
    15241 tttcatgggg tatttaaaag gcaaaagtgc actcatgatg tttgacaaac acgccaatct
    15301 caagtacaag tttgggaatc ggcatttctg ggcggaaggt tattatgtaa gtacagtagg
    15361 gcttaataaa gccacactta agaaatatag tcaagattaa agaaattatc gagtggatga
    15421 tttcttcacg agtatgaaaa tttgagaacg agtaaagtat gatatatagt aaaatgaaat
    15481 aagaacagga caaaatcgat caggacagtc aaatcgattt ttaacaatgt tttagaagta
    15541 gaggtgtact attccagttt caatctacta tagcactaga taaattgagt gtaaaagaat
    15601 atgaggatcc ctttagggat agtggtaagt aataccaaag tctcttaaag aggcaagtga
    15661 cgagtcaaga gcaataaggc ttgaacaacg tgaaagccag cgtctttagg cgctggctgg
    15721 taatttgggc ttatagctct gggataaacc acccgtttga cgggtggtta tgattttatc
    15781 tgagtgtaac atactgttgg gcaatctcgc tgatgcggtc aaagttgcct tgggaagcga
    15841 gtttattgag ttcgccacca attccaacgg cgtctgcacc agcagcgaac cattgaggga
    15901 tgttgtttag accgactcct ccggttacca ttacggaaac ttgtgggatc ggtgccttga
    15961 ctgcagagat atatgctgga ctgagagcac tacctgggaa gagtttgatg atttcactac
    16021 cggcttcaag tgcagtcgtg atctctgtga gggtaataca gcctggaatg tacggtgtgc
    16081 tgtagagatt gcacattttc gcagtttcag catggaaaga tggagaaaca acgtaatttg
    16141 ctccggctag aatggcatct ctagcagtta cggcatcaag cacagtacct gcaccgatac
    16201 aaacactctg atcgtcctga tacaagtcta caagttcctt gatgatttgt cctgcatact
    16261 gattggtata ggcgatttca atagctttga taccgccctt gatacaagca atcgaggctt
    16321 gcagtccttc ttcctttgta tttcctcgaa tgacagcgac aattttctgt ttttttagtt
    16381 caataatcgt atctgatttg gtcatgtaat tctcctaacg aatgatatct tgtgcatttg
    16441 ccagtaaatt ttcaatacta gttgcggaag tggagagatg gtctcctgga agagtgcatt
    16501 tgagagttgc gctcgcaact gcaaagtcaa tggtagtttt tagggaggaa tgatggagta
    16561 gttggtaaag ggcaccagat ataaaggcat ccccgctacc aattcgttga tagactgcag
    16621 tttttagttg gacagactct tcaaatcgtt cttccagagc ataggcttga tagacatttt
    16681 tgtcttgctc atcactagag cggagggtat ggaaaatggc cttgaaacca taggcttctt
    16741 ttaaaagtcg catgcgattt tccacctctt ctaggctagc actgtctctt ggaaacatct
    16801 ctagattttg gtcatcaatc atgagaggat caataccgaa gcaatagtca gtaaaacgtg
    16861 caaacttaga aaattcatac ttggcttcta ggactgaaat catctttgtt ctcagattga
    16921 gatccattga aacgacaatt cctcggcgct tggcttcttc caagagtagg agaaggatcg
    16981 cacggacctc ttgaccgata gctacggtga ttccactaaa atgaaaatgg ctaatcccct
    17041 gaaagagaga atccatatct agcatgtttg gccgaatctg gctgatactc gtatgcttac
    17101 gatcgtagaa aacttcactt tgacgacaac caaagccgtt ctccaaatag tagaggccga
    17161 ttcgatcgcc aagccgacag attgaactgg tatcgatttg gtgctgtttc aagaatgtga
    17221 gaaaacgatc tccaatctcg ttggcaggga gtgcggtaaa gactttcgtt gagataccca
    17281 gggcttgcaa attacaagcg atgttaattt ctgatccgcc aaaataagtc gaactggcaa
    17341 catgatcgcc gatactggtg gcatctaatg gtgagattcg aattaatggt tcgccaaaca
    17401 gtaatatttt cgtcatccat tagttccttt ctggtttaga atagctttac ttggctaata
    17461 cttctttcaa ataagcagcg acttcatcat cttggcagtt ttcaaagaag tattcttgga
    17521 aatatttgcc agagatggtg tctttgagga agtcttggtc gattgtttta aggatggtca
    17581 tcaaatcatt gtgggtgatt tgtttcacct cgtttaagat acgagcgttg cgttgttcag
    17641 ggattacacg ttctcttggg tagccaccgc ccatttcttc agcaaataag cgttcaaaca
    17701 tcaatttgag ggtcagttct gccccccagc caaatccttt ggcataaggg atagacaagg
    17761 cgttaccacc attgatttga gaataaaggt aagcgtcagt tgggtccact gctagaccac
    17821 agacaacacc agggaagctg tttaaagcaa gcatagcccc tacacccgta ccacagccgg
    17881 taacaacaaa gtcaactgcc tttgtattta aaaggatggc agccattagt ccgttctgca
    17941 cataagttaa ttgactttct ccttcttctc cacgcatacc atagttaaat aattggtagc
    18001 cttttttatc tgtcgcttct tttagactat cgtaaataat gtgattcttg ctagcttgac
    18061 tattttcatt gattaatgcg attttcataa gatcttcttt ctatttttac tcaggttgtt
    18121 ttccgatgta ggctaagata ccgccatcta catataggat gtggccattg acaaaattgc
    18181 tggcatcact agcgagaaag acagcagggc ccatcaaatc ttcaggattt ccccaacgtg
    18241 cagcaggtgt ttttgcaatg atgaactggt caaatgggtg gcgagaacca tcttcttgca
    18301 actcacgaag aggtgctgtt tgaggagtgg caatataacc cggtccaatt ccgttacatt
    18361 ggatattggc tccaccgtat tcagacgcaa tgttgcgggt caacattttc aagccccctt
    18421 tagcagcagc ataagcgcta actgtttcac gtcccagttc gctcatcatc gaacaaatat
    18481 tgataatctt tccatgccct ttctttatca tagaaggaat aactgccttt gaaacgataa
    18541 atggtgcgtt taagtcaata tcgatgacct tacggaaatc agcggcgctc atttcgcaca
    18601 ttggaactcg gcggataata ccagcgttat taacgaggat gtcaatgaca ccaacctctt
    18661 gttcgatttg cttgaccatg gcttggatac cgtcctcgtc tgtcacgtca cagacatatc
    18721 catgggcttc gatgccaact tcacgataag cttcaatccc tttattgacc agatcttgat
    18781 tgatatcgtt aaagacaata gtagcgccgg cttcagcgta ggatttggca atagcaaatc
    18841 caattccata agaagcgccg gtgatgagag caatttttcc ttttaaagaa aactgctcaa
    18901 ttgagaatga tgtatttgtc atttgatact tcctttcaaa tttgttaaga cttctctata
    18961 accaaattat attctagtaa accggttttg tcaataaata aatattttta tttataaaaa
    19021 gatttatttt ccttaaaaat ggaatatttt gaaatttctg tctattaaaa tgattaaaaa
    19081 gataatgaaa gcgctaatat ttttttaaaa aaactattga caagataaaa ataaaggatt
    19141 acaatgggga atataaagta aaccggtaaa cctaaacaag aaaggagaaa agatgaaaat
    19201 tgtacttgta gggcatggac attttgctac agggatttat agttctttac aattgattgc
    19261 aggcaatcaa gaaaatgtgg aggcaattga ctttgtggaa ggaatgtcag cagatgaact
    19321 caagcaaaaa atcttacttg caatttcaaa tgaagaagaa gttttaatcc taagtgatct
    19381 cttgggagga tcgccattca aggtttcttc taccataatg ggagaaaatc cagccaaaac
    19441 aatgaatgtt ctctcgggtt tgaacttagc catgttaatg gaagcagtct ttgctagaat
    19501 ggctcatagc tttgatgagg ttgttaataa atcagtagtg gcggcccagg gcggagtcgt
    19561 aaatggtaaa gaattgtttt caacggatgc agaggaagag gaagaagatt tcgaatcggg
    19621 tatttaaagg gtaaaagaat gataaaaaag gttacgattg aaaaaataaa atcgcctgag
    19681 cgcttcttag aagtaccact tctgacgaaa gaagaagtcg gccaggcaat cgataaggtt
    19741 attcggcagt tagaactcaa ccttgactat ttcaaggaag atttcccgac gccagctacc
    19801 tttgataatg tctatccaat catggataac acggaatgga ccaatggttt ctggacagga
    19861 gaactgtggt tggcttatga atacagtcaa caggatgcat ttaaaaacat cgctcataaa
    19921 aatgttcttt ctttcctgga tcgtgtcaat aagagagtag aattggatca ccatgatctc
    19981 ggcttcttgt acacaccgtc ttgtatggct gaatataaga taaatggaga tggagaggct
    20041 agagaagcaa ccttgaaagc tgcagataag ttgattgaac gctatcaaga aaaaggtggt
    20101 tttattcaag cttggggaga cttgggcaag aaagagcatt accgtttgat tatcgactgc
    20161 ttgctcaata tccaactctt attctttgct tatcaagaaa caggcgatca aaaatactac
    20221 gatattgcag aaagtcattt ctatgcttca gctaataatg taatccgtga tgacgcttcg
    20281 tccttccaca ccttctattt tgatcctgag acaggtcaac cctttaaagg tgtaacgaga
    20341 caagggtata gtgatgattc atgctgggca cgtggtcaat catggggagt ctatggtatt
    20401 cctttgactt atcgtcactt aaaagacgag tcctgctttg acttgtttaa gggtgtgacc
    20461 aattatttct tgaatcgtct gccaaaagat catgtgtcct attgggattt gatttttaat
    20521 gatggtagtg ctcaatcacg agattcttca gcaacagcta tcgccgtctg tgggattcat
    20581 gaaatgctaa aacatctccc agaggtggat gctgacaaag atatttataa acatgctatg
    20641 catgccatgc ttcgttcctt gatcgaacat tatgcaaatg atcaatttac ccctggtggg
    20701 acaagtctcc tccacggtgt gtactcatgg cattcaggta aaggagtgga tgaaggcaat
    20761 atctggggtg actactatta cctagaagcc cttatccgtt tctacaaaga ctggaaccta
    20821 tattggtagg aggagaaata tgacaatgcc aaatattatt atgacccgta tcgatgaacg
    20881 gttgattcat gggcaaggac aactttgggt aaaataccta ggttgtaata cggtcattgt
    20941 tgccaatgac gaagtaagca cagacaagat gcagcaaact ctgatgaaaa cagttgtgcc
    21001 agactcagtt gccatgcgtt tcttcccttt gcaaaaggtg attgatatca ttcacaaggc
    21061 taatcctgct caaacgatct ttatcgttgt aaaggatgtg aaggacgctt taaccttggt
    21121 agaaggtggt gtccctatca aagaaatcaa tattgggaac attcacaatg ctactggtaa
    21181 agagcaagtg acacgctcca tcttcctggg tgaagaggac aaggcggccc tcaaggaatt
    21241 gagccaaact catcaagtaa catttaatac gaaaacaact ccaacaggaa atgatggagc
    21301 tgttcaagtc aacattatgg actatattta aaagaggaga tcgttatgtc gattaatgta
    21361 tttcaagcga ttttaattgg attatggaca gctttctgtt ttagtggaat gctgttagga
    21421 atttacacca atagatgtat tgttctgtca tttggtgtcg gaattattct aggtgatttg
    21481 cctactgctc ttgcaatggg agctattggt gaattggctt atatgggatt cggtgttggt
    21541 gctggaggta ctgttccacc aaacccaatc ggacctggta tctttggtac cttgatggct
    21601 atcactagtg ctggtaaagt cagtccagaa gcggctcttg ccctctctac tccgattgct
    21661 gtggcgattc aattcttaca aactttcgcc tacactgtac gtgctggtgc gcctgaaacg
    21721 gcgatgaagc acttgaaaaa ccataatttg aagaaattca agttcactct aaatgcaaca
    21781 atttggttgt ttgcctttat tggatttacc ttgggttgct tgggtgccct ttcaatggat
    21841 accttgttga aactcgtaga ctacattcca ccagtattgc ttacaggttt gacagttgct
    21901 ggtaaaatgc tcccagctat cggttttgcg atgatcttgt cagtgatggc taagaaagag
    21961 ttgattccct ttgtcttgtt gggatatgtt tgtgcagctt atctaaacat cccaacaatt
    22021 ggtattgcaa ttgtaggtac tatctttgct ttgattgaat tttataacaa gccaaaaaca
    22081 gcggatcatg tggtagagga ggaagcacac gatgactgga tctaacaaat taacaaaacg
    22141 tgattatctt aaaacgtctt tgcgggcatt cttttgtcaa aatggattta actatagtaa
    22201 ctatcaaggg ttgggatatg ccaatgtgat gtatcctgct ttgaaaaaac actatggaga
    22261 ggatcaggaa ggtttctacc aagccttgga agaaaactgt gaattctata ataccaaccc
    22321 acacttcctg ccttttatta ccagcttgca tcttgtaatg ttggaaaatg gccgaccaac
    22381 aaaagaaaca cgtagcatca agatggcctt gatgggacca ttggcaggta ttggggattc
    22441 tctttctcaa ttctgtttag ctccattgtt ctcaaccatc gcagcttcgt ttgctcaaga
    22501 aggcttggtt gtcggtccaa tcttgttctt ccttgcgatg aatacgattt taacagcgat
    22561 taaattgtca actggtctgt atggatacaa actaggaaca actgtgattg ataaactaag
    22621 cgaacagatg gcaacgattt ctcgtattgc caatattatc ggtgtaaccg taattgctgg
    22681 tttggcagcg acatctgtta agattatggt gccgattacc tttgctgcag gggaagttaa
    22741 agcagacgct aaacaaagta tcgtaagtat tcagggaatg cttgataagg ttgctccagc
    22801 tcttctacca gccctattta cacttttagt ttactacttg atcaaagaaa agaaatggac
    22861 aacatataaa ctcgttattt taacagttat catcggaatt atcggaagct ggcttaagat
    22921 tatagcttaa tggatacaac attgttttat ggaatagtga ttgtcttggc agtgagtccc
    22981 cttttactgt caagctttca ttctattcgt cagcaaaagt tgcttcgcaa acagatggag
    23041 caacgacaag agtatttagc ttctttaaca tctggtgatg aagtgttgtt attgtctgga
    23101 attcatggaa aaatcatttc tatcaaagat gacttggtct ccttgcagat tgcaaaaggt
    23161 gtggtcatct atgtagaaaa ggaaagtgta atgggaaaga caaaagaact gctttttaag
    23221 tagttctttt tctggtaata gataatgaaa gaagaaagaa gacaattttt tgaaagaatc
    23281 gatggaaacc aatgtcgtga ttatatcttg tcccactgtt caaaagacta tgagaaagtc
    23341 aagagttccc ttgaacgctt gatggacaat cgttttatgt ttgatagtcc ttgggatatg
    23401 gagccttgtt caaaaatcca tcaaatccag ccgatggtat gggatcaagt atttgaagat
    23461 gatccagaat ggtcttatat gctcaatcga caagaatatc tcttgcagtt tatgataggg
    23521 tatctggtag aaggagataa ggactatatt caaaagtgca agttctttct atttgattgg
    23581 attgagcagg tgagagaatt ttctcctcaa tccttgatga ctagaacctt ggatacgggt
    23641 attcgttcct ttacttggtt gaaactactc ttgcttctct tgaaatttga cttgctagag
    23701 gagaaagaac tagagaaaat tttggtcagt ctagaaaagc agattgactt tatgaaaagc
    23761 tactatcgcg ccaagtacac ccttagtaac tgggggattt tacaaacaat tccgatgctt
    23821 gctatctatc atttcttttc agataagatg gacctagaag aagcttacca ttttgcttca
    23881 gaggagttga aacagcaaat tgagacacag attttaggag atggaagcca gtttgaacag
    23941 tcgattctct atcatgtaga ggtttataaa gccttgctgg atttgtgtct cttgcttcca
    24001 gacttgcaag atagttacca agagttgctg gaaaagatgg cgacctatat tcaaatgatg
    24061 acaggcttag atggacgaac tttggctttt ggtgatagcg attctacaga aacgacagaa
    24121 attttgagcc tgtctgctgt ggttttgaac aaggaagacc ttcttaacgg tctggatgtt
    24181 aaagttgatt tgcttagcct cttgttcctg gggcgagaaa aggtcaagcg actgcaggaa
    24241 tttgaaagga gagcttggca gcctaagtcc atgatctttg aagactctgg acatgtctgt
    24301 attaaggatg aacatcgtta tctatttttc aaaaatggtc cgctaggaag tgcccatagc
    24361 catagtgacg agaatagttt ttgcttacag tatcaaggcc aacctatttt catagatgct
    24421 gggcgttatt cttatcggga gatatatgaa cgttatctct taaagagtgc ttggagtcat
    24481 tcgacctgca ttgtagatgg gaaagctccg gaaagaatca caggatcctg ggaatatgaa
    24541 tactatcctc actccctgtt ttgtcaccat aaagaaaggg agggagtgca ttatattgag
    24601 ggggcttatt ggtcagcaga acctgatttg ccttatcttc acagaagaaa aatcctcatg
    24661 ttggtagagg atgtctggct cttggtggat gacatcaggt gtcaaggtca gcatgaggcg
    24721 ttgactcagt ttatccttga caaggatgtg acctatcaag atgggaaaat caatcagttg
    24781 agactatgga gtgaagttga ttttgatttg gaagatacca tcatttctcc taaatacaat
    24841 gagcttgaaa gaagtagcaa actcaccaag cgccaattct ttgagaatca gatgctggat
    24901 tataccatca ttgcgcatga gagttttgaa atcatccgtc attctgtcta ccagacagat
    24961 gatcgtgaag tggaaaatgc tctggctttt gaagtgaaaa atgacgaaac agacaagctg
    25021 attctgttat taagcgagga tattggtgta ggtgaaaaat tgtgcctcgt tgacggaaca
    25081 aaaatgcgtg gaaaatgtct agtatatgat aaaataaatg agagaatgat tcgcttgcag
    25141 tgctagaaat aggcattttg aatagtgaac atgttataat aagtattagt aggaggtgtt
    25201 ttagattgga gaagaaactg accataaaag acattgcgga aatggctcag acctcgaaaa
    25261 caaccgtgtc attttaccta aacgggaaat atgaaaaaat gtcccaagag acacgtgaaa
    25321 agattgaaaa agttattcat gaaacaaatt acaaaccgag cattgttgcg cgtagcttaa
    25381 actccaaacg aacaaaatta atcggtgttt tgatcggtga tattaccaac agtttctcaa
    25441 accaaattgt taagggaatt gaggatatcg ccagccagaa tggctaccag gtaatgatag
    25501 gaaatagtaa ttacagccaa gagagtgagg accggtatat tgaaagcatg cttctcttgg
    25561 gagtagacgg ctttattatt cagccgacct ctaatttccg aaaatattct cgtatcatcg
    25621 atgagaaaaa gaagaaaatg gtcttttttg atagtcagct ctatgaacac cggactagct
    25681 gggttaaaac caataactat gatgccgttt atgacatgac ccagtcctgt atcgaaaaag
    25741 gttatgaaca ttttctcttg attacagcgg atacgagtcg tttgagtact cggattgagc
    25801 gggcaagtgg ttttgtggat gctttaacag atgctaatat gcgtcacgcc agtctaacca
    25861 ttgaagataa gcatacgaat ttggaacaaa ttaaggaatt tttacaaaaa gaaatcgatc
    25921 ccgatgaaaa aactctggta tttatcccta actgttgggc cctacctcta gtctttaccg
    25981 ttatcaaaaa gttgaattat aacttgccac aagttgggtt gattggtttt gacaatacgg
    26041 agtggacttg cttttcttct ccaagtgttt cgacgctggt tcagccctcc tttgaggaag
    26101 gacaacaggc tacaaagatt ttgattgacc agattgaagg ccgcaatcaa gaagaaaggc
    26161 aacaagtctt ggattgtagt gtgaattgga aagagtcgac tttctaaaat gaaggaaaat
    26221 gacttgcaat ctctgttaag aaataaaata atcccaccta gaacaagcta ggtgggatta
    26281 tttgcctatg aaatgagaaa ttatgggagc aagctcctaa atcaactgtt tttgatctac
    26341 ttctttaact acttgataaa agttatagaa gtaggccaaa cttgaaatga tggttacgac
    26401 taggaatatt gaaaatttcc attggacagg ggtggttaaa agttgtggaa aggatatgag
    26461 gagaaagaag agggctgcgt tgaggacagg tatccgtttt gattgtattt tctcaagtcc
    26521 tttattgagc gcaggaagaa agaggagtag gagtagtaaa actgtatgag aaacagctcc
    26581 tgaaataagg gcgaagaaaa ggaaaatact gataaaaaca tgaatgatca gtagtctagc
    26641 tagtgatttc ataaggcacc tcctaatcct ggtctttttt agctcttgca atacgaagtg
    26701 agtcgacaat atgtatcatc actccgaaaa agaaagctcc cagtatagtt ttaaaaatat
    26761 gttttgtatt tagaagagaa ctgataaaat ttggattttc acttgttagg gtatcaatga
    26821 gtggaattat aaaaaatatc actgttccat aaatcgaacc tgctttcaga ccaggataac
    26881 gtaactgttt cttttctttt ttcatgagtt tcctcctaat cctcatcttg atttttctta
    26941 gtttttgcaa tgcgacggga gatgaggaac tgtatgctcg ctccgaagaa aatagaaccg
    27001 agaacacttg atacaccatt tcttatagtg agaagagaat gaaaatagtc ctgaccttca
    27061 tctatgagta tactgagaag aggagttata aaaaacatcc atagaccaaa gaacaaacct
    27121 gctttcagac ctgggtagtg tagttgcttg ctttctttct cattcagcat atctggttca
    27181 atgactgtga tgcctgtttt tttcatttgg taggtgacat agccagcagc gatgagggca
    27241 atcactaaaa tcagaggagg atagattaga gccacttctt gagggtattt ataggccaga
    27301 aggagtggaa taagatttcc gaaaatcatc agataaaaga ggatgataaa aacttggttc
    27361 ccaatactat cggcctcacg ccgtttgtat tcgtcaaggg gaccagaaat accgtatgtg
    27421 cgtttgatca gtttttcagt gaaggtttct tttttcataa gtttgctcct tttttaaaaa
    27481 tcttcctccc aaaagagact gttgaggtca gtttggaggc tgcgggcgag attgagacag
    27541 agttccaagg ttggattgta cttgtcgttt tcaatcatat tgatagtctg tctcgagaca
    27601 ccgatatcct tggcgagttc gagctgggaa atacccaatt ccttgcgaaa ttctttcaca
    27661 cgattcatct gttctccttt ctgatttatg tcgtatatat ttgactatat tatagtcttt
    27721 taaacataaa gtgtcaagta tttttgacat attttttgaa gaaatagtag tctccttgtc
    27781 ctatttgtct gacaagtgca agctggtcgg atttgtggta aaatagataa gatatgacaa
    27841 aagaatttca tcatgtaacg gtcttactcc acgaaacgat tgatatgctt gacgtaaagc
    27901 ctgacggtat ctacgttgat gcgactttgg gcggagcagg acatagcgag tatttattaa
    27961 gtaaattaag tgaaaaaggc catctctatg cctttgacca ggatcagaat gccattgata
    28021 atgcgcaaaa acgcttggca ccttacattg agaagggaat ggtgaccttt atcaaggata
    28081 acttccgtca tttacaggca cgtttgcgcg aagctggtgt tcaggaaatt gatggaattt
    28141 gttatgactt gggagtgtct agtcctcaat tagaccagcg tgagcgtggt ttttcttata
    28201 aaaaggatgc gccactggac atgcggatga atcaggatgc tagtctgaca gcgtatgaag
    28261 tggtgaacaa ttatgactat catgacttgg ttcgtatttt cttcaagtat ggagaggaca
    28321 aattctctaa acagattgcg cgtaagattg agcaagcgcg tgaagtgaag ccgattgaga
    28381 caacgactga gttagcagag attatcaagt tggtcaaacc tgccaaggaa ctcaagaaga
    28441 aggggcatcc tgctaagcag attttccagg ctattcgaat tgaagtcaat gatgaactgg
    28501 gggcggcaga tgagtccatc caccaggcta tggatatgtt ggctctggat ggtagaattt
    28561 cagtgattac ctttcattcc ttagaagacc gcttgaccaa gcaattgttc aaggaagctt
    28621 caacagttga agttccaaaa ggcttgcctt tcatcccaga tgatctcaag cccaagatgg
    28681 aattggtgtc ccgtaagcca atcttgccaa gtgcggaaga gttagaagcc aataaccgct
    28741 cgcactcagc caagttgcgc gtggccagaa aaattcacaa gtaagaggga aaaagatggc
    28801 agaaaaaatg gaaaaaacag gtcaaatact acagatgcaa cttaaacggt tttcgcgtgt
    28861 ggaaaaagct ttttactttt ccattgctgt aaccactctt attgtagcca ttagtattat
    28921 ttttatgcag accaagctct tgcaagtgca gaatgatttg acaaaaatca atgcgcagat
    28981 agaggaaaag aaaaccgaat tggacgatgc caagcaagag gtcaatgaac tattacgtgc
    29041 agaacgtttg aaagaaattg ccaattcaca cgatttgcaa ttaaacaatg aaaatattag
    29101 aatagcggag taagatatga agtggacaaa aagagtaatc cgttatgcga ccaaaaatcg
    29161 gaaatcgccg gctgaaaaca gacgcagagt tggaaaaagt ctgagtttat tatctgtctt
    29221 tgtttttgcc atttttttag tcaattttgc ggtcattatt gggacaggca ctcgctttgg
    29281 aacagattta gcgaaggaag ctaagaaggt tcatcaaacc acccgtacag ttcctgccaa
    29341 acgtgggact atttatgacc gaaatggagt cccgattgct gaggatgcaa cctcctataa
    29401 tgtctatgcg gtcattgatg agaactataa gtcagcaacg ggtaagattc tttacgtaga
    29461 aaaaacacaa tttaacaagg ttgcagaggt ctttcataag tatctggaca tggaagaatc
    29521 ctatgtaaga gagcaactct cgcaacctaa tctcaagcaa gtttcctttg gagcaaaggg
    29581 aaatgggatt acctatgcca atatgatgtc tatcaaaaaa gaattggaag ctgcagaggt
    29641 caaggggatt gattttacaa ccagtcccaa tcgtagttac ccaaacggac aatttgcttc
    29701 tagttttatc ggcctagctc agctccatga aaatgaagat ggaagcaaga gcttgctggg
    29761 aacctctgga atggagagtt ccttgaacag tattcttgca gggacagacg gcattattac
    29821 ctatgaaaag gatcgtctgg gtaatattgt acccggaaca gaacaagttt cccaacgaac
    29881 gatggacggt aaggatgttt atacaaccat ttccagccct ctccagtcct ttatggaaac
    29941 ccagatggat gcttttcaag agaaggtaaa aggaaagtac atgacagcga ctttggtcag
    30001 tgctaaaaca ggggaaattc tggcaacaac gcaacgaccg acctttgatg cagatacaaa
    30061 agaaggcatt acagaggact ttgtttggcg tgatatcctt taccaaagta actatgagcc
    30121 aggttccact atgaaagtga tgatgttggc tgctgctatt gataataata cctttccagg
    30181 aggagaagtc tttaatagta gtgagttaaa aattgcagat gccacgattc gagattggga
    30241 cgttaatgaa ggattgactg gtggcagaat gatgactttt tctcaaggtt ttgcacactc
    30301 aagtaacgtt gggatgaccc tccttgagca aaagatggga gatgctacct ggcttgatta
    30361 tcttaatcgt tttaaatttg gagttccgac ccgtttcggt ttgacggatg agtatgctgg
    30421 tcagcttcct gcggataata ttgtcaacat tgcgcaaagt tcatttggac aagggatttc
    30481 agtgacccag acacaaatga ttcgtgcctt tacagctatt gctaatgacg gtgtcatgct
    30541 ggagcctaaa tttattagtg ccatttatga tccaaatgat caaactgctc ggaaatctca
    30601 aaaagaaatt gtgggaaatc ctgtttctaa agatgcagct agtctaactc ggactaacat
    30661 ggttttggta gggacggatc cggtttatgg aaccatgtat aaccacagca caggcaagcc
    30721 aactgtaact gttcctgggc aaaatgtagc cctcaagtct ggtacggctc agattgctga
    30781 cgagaaaaat ggtggttatc tagtcgggtt aaccgactat attttctcgg ctgtatcgat
    30841 gagtccggct gaaaatcctg attttatctt gtatgtgacg gtccaacaac ctgaacatta
    30901 ttcaggtatt cagttgggag aatttgccaa tcctatcttg gagcgggctt cagctatgaa
    30961 agactctctc aatcttcaaa caacagctaa agctttggag caagtaagtc aacaaagtcc
    31021 ttatcctatg cctagtgtca aggatatttc acctggtgat ttagcagaag aattgcgtcg
    31081 caatcttgta caacccatcg ttgtgggaac aggaacgaag attaaaaaca gttctgctga
    31141 agaagggaag aatcttgccc cgaaccagca agtccttatc ttatctgata aagcagagga
    31201 agttccagat atgtatggtt ggacaaaagc gactgctgag acctttgcta agtggctcaa
    31261 tatagaactt gaatttcaag gttcgggctc tactgtgcag aagcaagatg ttcgtgctaa
    31321 cacagctatc aaggacatta aaaaaattac attaacttta ggagactaat atgtttattt
    31381 ccatcagtgc tggaattgtg acatttttac taactttagt aggaattccg gcctttatcc
    31441 aattttatag aaaggcgcaa attacaggcc agcagatgca tgaagatgtc aaacagcatc
    31501 aggcaaaagc tgggactcct acaatgggag gtttggtttt cttgattact tctgttttgg
    31561 ttgctttctt tttcgcccta tttagtagcc aattcagcaa taatgtggga atgattttgt
    31621 tcatattagt cttgtatggc ttggtcggat ttttagatga ctttctcaag gtctttcgta
    31681 aaatcaatga ggggcttaat cctaagcaaa aattagctct tcagcttcta ggtggaatta
    31741 tcttctatct tttctatgag cgtgggggcg atatcctgtc tgtctttggt tatccagttc
    31801 atttgggatt tttctatatt ttcttcgctc ttttctggct agtcggtttt tcaaacgcag
    31861 taaacttgac agacggtgtt gacggtttag ctagtatttc cgttgtgatt agtttgtctg
    31921 cctatggagt tattgcctat gtgcaaggtc agatggatat tcttctagtg attcttgcca
    31981 tgattggtgg tttgctcggt ttcttcatct ttaaccataa gcctgccaag gtctttatgg
    32041 gtgatgtggg aagtttggcc ctaggtggga tgctggcagc tatctctatg gctctccacc
    32101 aagaatggac tctcttgatt atcggaattg tatatgtttt tgaaacaact tctgttatga
    32161 tgcaagtcag ttatttcaaa ctgacaggtg gtaaacgtat tttccgtatg acgcctgtac
    32221 atcaccattt tgagcttggg ggattgtctg gtaaaggaaa tccttggagc gagtggaagg
    32281 ttgacttctt cttttgggga gtgggacttc tagcaagtct tctgacccta gcaattttgt
    32341 atttaatgta agaatggcat cctgatgttt cagggtgttt ttgtatttaa atacacaatg
    32401 aaaatcaaag aacaaactag aaagctaact ttaggctgct caaa
//
LOCUS       DAVBCN010000017        31003 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVBCN010000017 DAVBCN010000000
VERSION     DAVBCN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31003
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.017"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(297..515)
                     /locus_tag="VQ497_000720"
     CDS             complement(297..515)
                     /locus_tag="VQ497_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2517064.1"
                     /translation="MVLNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
     gene            complement(761..1039)
                     /locus_tag="VQ497_000721"
     CDS             complement(761..1039)
                     /locus_tag="VQ497_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517065.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEMLELACWFRVIIVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAAYSIHFNNGYCKQHTNEFKDSLKSLIL"
     gene            complement(1253..2884)
                     /gene="rlmD"
                     /locus_tag="VQ497_000722"
     CDS             complement(1253..2884)
                     /gene="rlmD"
                     /locus_tag="VQ497_000722"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET2517066.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSLHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWCTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSQKNASLNKITNAHYVCDTAENAMKKWLKEGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2958..3932)
                     /locus_tag="VQ497_000723"
     CDS             complement(2958..3932)
                     /locus_tag="VQ497_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HET2517067.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(4006..4620)
                     /gene="pmp23"
                     /locus_tag="VQ497_000724"
     CDS             complement(4006..4620)
                     /gene="pmp23"
                     /locus_tag="VQ497_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HET2517068.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(4620..5594)
                     /locus_tag="VQ497_000725"
     CDS             complement(4620..5594)
                     /locus_tag="VQ497_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HET2517069.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQKENGTYSILIKNIEDIQSK"
     gene            complement(5602..6858)
                     /locus_tag="VQ497_000726"
     CDS             complement(5602..6858)
                     /locus_tag="VQ497_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET2517070.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            complement(6920..7351)
                     /locus_tag="VQ497_000727"
     CDS             complement(6920..7351)
                     /locus_tag="VQ497_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2517071.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(7353..7955)
                     /locus_tag="VQ497_000728"
     CDS             complement(7353..7955)
                     /locus_tag="VQ497_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HET2517072.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7939..8778)
                     /gene="prmC"
                     /locus_tag="VQ497_000729"
     CDS             complement(7939..8778)
                     /gene="prmC"
                     /locus_tag="VQ497_000729"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET2517073.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(8778..9857)
                     /gene="prfA"
                     /locus_tag="VQ497_000730"
     CDS             complement(8778..9857)
                     /gene="prfA"
                     /locus_tag="VQ497_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET2517074.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9854..10444)
                     /locus_tag="VQ497_000731"
     CDS             complement(9854..10444)
                     /locus_tag="VQ497_000731"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HET2517075.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRGLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            10567..10749
                     /locus_tag="VQ497_000732"
     CDS             10567..10749
                     /locus_tag="VQ497_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HET2517076.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10886..12259
                     /gene="mnmE"
                     /locus_tag="VQ497_000733"
     CDS             10886..12259
                     /gene="mnmE"
                     /locus_tag="VQ497_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET2517077.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEANLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLIEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(12306..13241)
                     /gene="dapA"
                     /locus_tag="VQ497_000734"
     CDS             complement(12306..13241)
                     /gene="dapA"
                     /locus_tag="VQ497_000734"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET2517078.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKTIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(13297..14373)
                     /locus_tag="VQ497_000735"
     CDS             complement(13297..14373)
                     /locus_tag="VQ497_000735"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET2517079.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPCDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14715..14787)
                     /locus_tag="VQ497_000736"
     tRNA            complement(14715..14787)
                     /locus_tag="VQ497_000736"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14752..14754),aa:Thr,seq:ggt)
     gene            complement(14887..15396)
                     /locus_tag="VQ497_000737"
     CDS             complement(14887..15396)
                     /locus_tag="VQ497_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HET2517080.1"
                     /translation="MKKLTIRDIADIAIVTAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(15394..15490)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            15519..15950
                     /locus_tag="VQ497_000738"
     CDS             15519..15950
                     /locus_tag="VQ497_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET2517081.1"
                     /translation="MKIPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKAYIFRK"
     gene            16032..16409
                     /gene="mscL"
                     /locus_tag="VQ497_000739"
     CDS             16032..16409
                     /gene="mscL"
                     /locus_tag="VQ497_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET2517082.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(16451..17545)
                     /gene="hemH"
                     /locus_tag="VQ497_000740"
     CDS             complement(16451..17545)
                     /gene="hemH"
                     /locus_tag="VQ497_000740"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET2517083.1"
                     /translation="MKKAILMMTFGSPEEISFEGVAEFFTNIRRGVRPQDHEIQTLYD
                     NYIRIGGTPLQRITREEVNLVKERLGEEYGIYFANKFSRPFIPDVIKQMETDGVEECI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEVLLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            complement(17623..18455)
                     /gene="srtG2"
                     /locus_tag="VQ497_000741"
                     /pseudo
     CDS             complement(17623..18455)
                     /gene="srtG2"
                     /locus_tag="VQ497_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218039.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG2"
     gene            complement(18533..19531)
                     /gene="srtG1"
                     /locus_tag="VQ497_000742"
     CDS             complement(18533..19531)
                     /gene="srtG1"
                     /locus_tag="VQ497_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG1"
                     /protein_id="HET2517084.1"
                     /translation="MKTKREKPKKSLSRRLVLAVDGVINHLLLIFAALIFLFGFYALW
                     DSNQVYSLASSSEYEAYRPVTTQQDELASFSGFSKLQELNPEVLGWINVYGTNIDYPL
                     VQAKDNEKYLNKDSKGEFAATGAIFLDARNNPKFEDFNTIIYGHHVENGVMFGDVAKF
                     ADQEFFDQHRYGSIYYNGVEKGLEIFEMLEVDAYDFNIYDPGIQGEDRQQAYLDHLLS
                     VAMHKRDISLSPSDRIILLSTCFLDVTNGRHIVVAKITDTVPKNTFHTKKSKPFPYSV
                     FDDSSLGRFLSSIPLWIWYLILFVLFLLLIFLLLVLYLILRRRRESKKNARSRPFY"
     gene            complement(19549..20745)
                     /gene="pitB"
                     /locus_tag="VQ497_000743"
     CDS             complement(19549..20745)
                     /gene="pitB"
                     /locus_tag="VQ497_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus major pilin PitB"
                     /protein_id="HET2517085.1"
                     /translation="MKKTFFKKLFTASIAAITALSVFRGVPTFADDNSAITKANGENN
                     AVVKINKTLNIAEGITTPTATFTFKFTEKTGQSSNGAPYQTGVAIPDRNVEYNKNDHP
                     TADKIQKATEDIFSGVAYGHAGEYVYDVAEAKTGWQAITKNGKTIDAMRYDKRTYEMH
                     VIVKNKVNGGVYISSVYFKENNKSNAPKVEPSEQGVYNLFDNTYTKDASKEPNPDDPS
                     QVDPNAKALTITKKVDGASGDKTRDFQFHIKIQLPSTNKTAETPVTNIIVKHGSKSEV
                     LAVVTPADTVEYNFTLKDGETFTVEQLPAGSKYTVTETGVAGYTDSSIYTTNGAEQTS
                     QGQKNVDFTLTDILIGEKKNDNKVTNKIDDVTPTGLLIDNLPFILMIGLGLAGFVVLS
                     KKRREA"
     gene            complement(20790..21341)
                     /gene="sipA"
                     /locus_tag="VQ497_000744"
     CDS             complement(20790..21341)
                     /gene="sipA"
                     /locus_tag="VQ497_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system signal peptidase SipA"
                     /protein_id="HET2517086.1"
                     /translation="MLLKKKHKKPVTQVNRDKSPPSVWGDILYLVSKLLMVGFVLAIL
                     YFFVFGLLRYNDDGMKPALKDGDLVVYYRLDKRYSIGDLLVYSYKGKERVARVIATEG
                     STIDINENGLIINGSPQQEQDIYKETLLYKEGATFPMKVPAGQLFVLGDNRTTAVDSR
                     AFGTIPIQDTQGKVVTVIRRRGF"
     gene            complement(21341..23101)
                     /locus_tag="VQ497_000745"
     CDS             complement(21341..23101)
                     /locus_tag="VQ497_000745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517087.1"
                     /translation="MIDTPYWFTSGLERGMLNISSLIAPKIDGFYSIKFRYIGSNDSI
                     TSLKGYISGYNSGIPNEIFSANNENDLQQKFKEITDKILPLGVHHVTISDVLSKYVQL
                     LPGDASHLRVVKIKDGNEQELNDNQVTIETKKNEQGLVEVTAKFNPSYTLEDDAKYVL
                     KFTVTSSQEAFDAIAGDKTLTSDDAEEADATKLYSNKGAKVAYSYGIGTSRTKIKDYS
                     EKPTFKPSDPLTVPVEIEWKGVDGKSNPSANRPPSVELNLNQKKDGSIKDSYRKVTSP
                     VQTNSFTENTSFAKVAKGYDYELKAPDAPGYTVEVQKTGTKEKPSFKVIYRQLPSLTV
                     KKILEGEQSPNKSFTINVTFSDKDGKPINGKFGNTTVTNGKAQISLKNSQETALSYLP
                     RDTHYKVEEVENSRTGYHVTYEKQEGTLSEDVQTIVTNHRLPTLSVTKKVTGAFANLL
                     QSFKITINVKDAQNKPLNGSYSAIVNNQKTTLQFTNGKATVDLKKDKTIKILDLPLNA
                     RYSIEEEASSSRGYQVSYDKKEGTLDANKSATVTNNKNSVPETGIDFLSSTLVLGVVL
                     PLGGIFFIILLGHLVVNRRK"
     gene            complement(23423..23926)
                     /locus_tag="VQ497_000746"
     CDS             complement(23423..23926)
                     /locus_tag="VQ497_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HET2517088.1"
                     /translation="MNVQYDFKKIQYFTSSLVIFLAILFLCAPINSLRADSITEPQTT
                     LHKTITPISGQKDQYELSLDITSKLGTETQSEPLDVVLVADLSGSMEERDVWSYSSRR
                     YISRIEALKHTLKGVNGRQGLIDTILSNSQNRLSIVGFAGKIDNQYNDRYYNEYYLSY
                     QYGTWPN"
     gene            24285..25508
                     /gene="pepT"
                     /locus_tag="VQ497_000747"
     CDS             24285..25508
                     /gene="pepT"
                     /locus_tag="VQ497_000747"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HET2517089.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPRQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(25544..26173)
                     /locus_tag="VQ497_000748"
     CDS             complement(25544..26173)
                     /locus_tag="VQ497_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517090.1"
                     /translation="MNKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSANSKDKTALLESTKAILVLNHQIQSKVSASEETSPARNAEANSDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            26138..26359
                     /locus_tag="VQ497_000749"
     CDS             26138..26359
                     /locus_tag="VQ497_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517091.1"
                     /translation="MDLFQELICLVQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(26630..26978)
                     /locus_tag="VQ497_000750"
                     /pseudo
     CDS             complement(26630..26978)
                     /locus_tag="VQ497_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(27083..27643)
                     /locus_tag="VQ497_000751"
                     /pseudo
     CDS             complement(27083..27643)
                     /locus_tag="VQ497_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(27869..30988)
                     /gene="phtE"
                     /locus_tag="VQ497_000752"
     CDS             complement(27869..30988)
                     /gene="phtE"
                     /locus_tag="VQ497_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HET2517092.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQISQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         8833 a         6360 c         5686 g        10124 t
ORIGIN      
        1 aaaggatcta gtttttattt ttagttaaat tatgtctata tttttcatta actcatcaat
       61 aaaaatttgt ttgctaatat ctctttcttc tgattttgtt tcaaggatta ttgctacatc
      121 attcatattt ttaggtaaat accaaccatc tggcttatct gaatatccct tgaagcctaa
      181 ttgattaaaa gtagttattt gaccagtacc ttgattgatg ttttcttcaa cttcattaaa
      241 gcctatactt ctcttgcata atccctaact tgatcttctg tccttaaagt cattttctat
      301 tcctcgtcac taaaattatc ttcaaaacta ataatatcat taggagtgca agataaagcc
      361 ttacatattc tttctaaatt cttaaatgta attggtttat cctttcccat ttgtgccata
      421 acatttgttg taagtcctgc catagctatt acatctgttt tctttagtcc tttttttgct
      481 aactgtatcc ataatggttt atagttaagt accataatat ccctcttttt ttcttcatta
      541 aatttattta attatagcat aaatattgat ttcattcaat cttacattga tttagtgatt
      601 tgttttagag aatttgttgt gttattccgt ccttattttg ccataaattg ttttaagtgg
      661 gacctcttat ccttaccatt tttacctcct aattttgagt ataaaaaaga cgatagagtt
      721 tttaatttct atcgtctgag atttacatat taaattttca tcacaaaata agactcttta
      781 aagaatcttt aaattcattg gtatgctgct tacaatatcc attattaaaa tggatactat
      841 aagcagcaca ccaactattt attttttgag ttttaaactt tggtagaagt cttgaactcc
      901 ttatatttac ataactattt gcctctataa ctattatgac acgaaaccaa caagcaagtt
      961 caagcatctc catatcttta ttcaaatgag gactaagata ttcacaaact ttatcataaa
     1021 cctctttatc ttttttcata aaaggggcat acaacttagc aaatttatcc taaaattttt
     1081 tgttgccttt atcaatcatt tcaacgctcc tttaaatata aaaataatcc tatcaatctt
     1141 tatattgagc ctattttcta attgatacct tgcaagaaag gaataatagc tgttcctata
     1201 ccaagtagga atgtaataaa tgcagaaagt catatactgt cattcttttc tattaaatca
     1261 ttttgaagtg tctcaaagca tccatgatgg cttcttcttt ttcaggtgta cactgtggaa
     1321 taatttgttt atccttttta gacttgttgt aatgttctcg taattctatt ccacatttct
     1381 tttttatctg tgcaatatat aatgtcgaaa cttttaattc aaatttattc caaacatatt
     1441 ctttgatttg agcatatgtt gctttgctct ccgcacttgt caaatccatc tcatccagct
     1501 caatttcaac acttatgtgc ttatcgacat cgagtttgga caaaagtgct accgtctcga
     1561 cgtgatgcgt ttgaggaaat agatccaccg gctggacttt cttcaattca tatcccaatt
     1621 cttggtagag tttgatatca cgcgccatgg ttgcgacatt gcaggagata taggcgatgc
     1681 gatcggctcc tgtttgggcg cttgctttga taaagctttc tgtcaagccc ttgcgtggag
     1741 gatcaaccaa gataacggtt ggttgaatac cttccttgag ccatttcttc atggcatttt
     1801 cagccgtgtc acagacatag tgggcattag taatcttgtt caaagaagca ttcttctggc
     1861 tattctcaac cgcttctgga atcagttcaa caccgtagac ttctttgaca tgcttggcga
     1921 ctgataaacc aatggttcca ataccagaat aagcatcaat aaccacgtca tcttttttta
     1981 actctgcaaa gtcaatggct gtttgataga gtttctccgc catttcagta ttgacttggt
     2041 aaaaggctgg gccagcgatt tggaagtcat ttcccaacat ctggtccgta atatagtctt
     2101 gaccataaag agtgcaccac tccttaccaa aaatcgcatt ggtattctgg tcgttgatat
     2161 tttgcatgac agacacaatc tctgggaact gcttgataac ttgttcaatc aattggtcaa
     2221 cacgaaaaac ttttggacga gttgtcacca aaacgaccat gatttgtcct gaatagtgac
     2281 cacgacgcac cacaagattc cgaatcaatc cagactgttc cttttcgtca taaggtttta
     2341 aatcaaaacg acggagcagg tctcgaagag ctactacgac ttggtcaatg acaggatcct
     2401 ggataaagaa atcttcaagg ggcatgaggt tgtgcgaatt cttacggaaa aatcctgttt
     2461 ccaagacacc attcactcga cgaacgggca cctgcgcctt attgcgatac ttgactggat
     2521 gttccatacc aagcgtttca gcaacttcta catctgcaat tccagcaatc ttgtagagac
     2581 tgtccttgac ttgcttggtt ttaaacttga gctgttctgg ataagaaagg tgtcctaaat
     2641 ccgcgattcc tgaacgcagg taagccaaat ctagatcttg attacggtgt agtgactgga
     2701 caaggtattt ttcaactttt ccaaagccaa tctttttatt gaccttgagg acacgcatga
     2761 gaattttttc actcggtaaa gcattctcta caaaaaagac caaaccatct accttggcaa
     2821 ctcctgcccc ttcatgggtc aaatcaacaa tttcaacttc tacaatatca tttttcttta
     2881 acattctttt ctctttctat tggccgacaa aaaagacggc aacgttacga ttaccatcca
     2941 ttataccatg aaatttctta agaaccaaaa ctcttgaaga agttgacaag ggcttgccag
     3001 agggagctta agaagttacc accgtcctct agcgccttat ccgcatcaaa gttaaggttg
     3061 atatttttaa aactgtcgcc agcttgtgat acgatgcttt gtttaaggtc atttagggtt
     3121 ttagtgaaat ctgcattgct gaggatatca ctctttgaga gattcaaggc aaaattgatg
     3181 atgatattga tctggtttcc tgttatgacc tgatcaagtt tgtaattttt taaggtatct
     3241 tcaacaatct tgcggacatc ttcttctgtc agatttccct tgctttcttt agctttggcg
     3301 agtcctgact tgatatcagc tagggcaacg tttaatttat tagcatcata gcctgatttg
     3361 tccttgtttt cagcattgat atctgacaaa gcctttagct cttcttgagc caaatcttta
     3421 ttggcttgcg gcaccttggc tccattagct tctagcgaat agtagatccc agccaaagca
     3481 ctctcacctg taactggaat aggggctgct acagtgattt tggcatgttc catacccagc
     3541 gttactgctg cgtttcggta catatcctga gtcaccttag tgatattttc tggtgtttca
     3601 atcttgacct caagtggcga tttgtcacct agcttttgaa tcttggctga tgaatacaac
     3661 tgtaagctag agtcattggc cacattcatg attttagaat aaacatcagg tgtcatggtc
     3721 ttgagttctt tggtatctgt tgaggcattg tagcccagtt ttttaagagt ttgatttttt
     3781 tggtcttcag atagggagga acctaggaca tattcaggtt ggacataggt ttcatcgata
     3841 actttttgaa catctgttgc tgcatggacg ctattcatag ctgttactgc ccacaagacc
     3901 gcagcactgg tcagaaagag tttctttctc atagggaatt tcctccttta cttttctaga
     3961 gtaatatatc tatcttaaag aaaacttata acaaaaacac ctggtctagc cagatgttga
     4021 aaagagagtg aaacatttga tgatgtaaag gttaagtcgt acctgtctag aataataata
     4081 gtttcctcca tttacataga gttcagcacc gtgaaaaatg gaaatggggt gaatataact
     4141 ataagtcttt ccagtcgtat taccaagcaa gggggcaaca gtctcacgag agtactgttt
     4201 ggctagagcc aaggtatttt ccttgccatt ttgggcgata aaatcgatat aggcaggtcc
     4261 aaaattatag gcttgaacag ctgtccagat atctaccccc ttcttctgcg ccagatagag
     4321 attgcctgtc agagtttgaa tgccttgccg aatgctagag gcattatcat tgatggtgtt
     4381 ggttgaacca cttgcagact cactagactg cataacatcg ccttcttttc cttttgtttc
     4441 agtataaatc atagcaagca caagctcttc gtttgctggg gtgtcttttt cactcaagat
     4501 ttctcgcacc atgggttgat aggtcatgac ttgtttgaca tcttgatgaa cgcggtaagc
     4561 tttatagcca gcaaaaagga agactgctag tacaagcact cttcgaattc gtttaaacat
     4621 tatttacttt ggatatcctc gatatttttg attaagatag agtaggttcc attttccttt
     4681 tggataaact caacagactc ggcgtcttga tagacgttat tgggaacgat gagctcaatt
     4741 ccatttgata aggagagttt ttggttttca aatttcttta attggcgact ggcatcaatt
     4801 tcatcaaatt gaacaggttc tggtacggct tctttgactt ggtcaataaa gctcaaacga
     4861 gccgtcagat tgttgtcaaa aaggtcatta gccaatttct caggtgacaa ttcattgctt
     4921 tcttctaggt tgttgaaaat cgctgatttg accttggatt gaaattgaaa atcatctgtg
     4981 ttaaaagatt cagcaattct ctgggctgtt ttttccagtt ccttgataga ttttttagga
     5041 gaaatcttag gagcgacagc aagaagatta tctgaaaaat agttcaaaaa agtcccgttg
     5101 tacttgattc gtttttcaat caggtgatac ttgcgactct gaagattgac caccaaggcc
     5161 tcgtcagcac ccgttccaaa tccaggcagg ttattctgag tcagcttgat tggattatca
     5221 acttctcctc cgaggtgggt caaggtctcc cgcagggcaa ttcgcaagaa agcgaaatgt
     5281 tctacacctt ctttagaaaa ttgaacaaaa atcaagtcat tggtcttgag attttcagaa
     5341 atgctaaact cctctttcca gagattagcc agcgttactg atgtctccaa caaatcgtct
     5401 gtaatatgat tgaagaaggg attttcttct tcgaaaatcc cagtcttggc ttcatctgaa
     5461 tacacatgtt caattttttt acgtaggtat tcttcgattt ttggagtaat attgagaaac
     5521 ttatctgcta agaacagctc ggtatcatcc ggactgaact ggtgaataat ggctttctta
     5581 atataaatgt ccataaaagt tttattcctc gtataatggg aaggcatctg tcaattcttt
     5641 gactgcactt ctcacttctt ctaatacagc ctcattttct gaattcttaa gggttttaat
     5701 gatgagttca gccactttgc gactttcttc ttcaccaaat ccacgtgcag taatagctgc
     5761 tgctccgata cgaatcccac ttgtcttgaa tggtgacaag gtttcgtaag ggattgaatt
     5821 tttatttagg gtaatattga cttcatccag caagttttga gcaacttttc cgttttctac
     5881 aaccttagtc acatcaacaa ggaagagatg gttttcagtt ccacctgaaa taatacggaa
     5941 atcagggtct tgcaagaaga catctgccat agccttgctg ttcttaatta cattggcagc
     6001 atattccttg aaggctggat ccaaaacttc tttgaaggaa actgccttag ccgccacaac
     6061 atgctctaaa ggaccgccct gaatacctgg gaaaatagct gaattgattt ttttagcaag
     6121 ttcttcgtca ttggtcaaaa tcaaaccacc acgaggtcca cgaagggttt tgtgggtcgt
     6181 tgttgtagtg atatgagcgt atggcactgg gcttgggtga agaccagctg caaccaagcc
     6241 agcgatgtgg gccatatcaa ccataagctt cgcaccgaca gcatctgcga tttcacggaa
     6301 ttttgaaaag tcaataattt gagaataggc tgaagcacca gctacaatca gttttggttt
     6361 tacttcttgg gcttgtttca agatagcatc aaagtctaag agttccgttt caggatcaac
     6421 actataagaa acaaagttgt aggtttgacc agagaagcta acaggagccc catgagtcaa
     6481 atgaccacct gatgccaaat ccattcccat aaccgtatca cctggctcaa tcaaggacat
     6541 gtaagccgca cagttggcct gacttcctga gtgaggttgg acattggcaa atttagcacc
     6601 gaaaatttct tttgcgcgtt caatagcaag agtctctaca acgtctacta catcagttcc
     6661 accataataa cggcgtcctg ggtaaccctc ggcatattta tttgtcaaga tagacccttg
     6721 agctgccata acagccttgg aaactacgtt ttccgaagca attaactcga tattattttg
     6781 ttggcgttct tcttctttgg caatagcatt ccagagatca gcatcatatg ctttaaaatc
     6841 atctttgtca aaaatcatag gtcttctcct ttattgtgtg actagtccat tagtttgatt
     6901 ttacaataag aaaatcaaac taaaagatgc gaataaaccg tttctgcatt ttatcacaag
     6961 tatagccaac tttttcataa aatgcatgag cacccagacg atgattggca gaatttaagc
     7021 ggataaaccc ataaccacat cttttggctt cttgttccaa cccttgtagt aaacttttac
     7081 cgataccttg accttgcgct tgaggtgaaa ctgctaaagc taagatatta aatcctgctt
     7141 tggaatagag tgattcgtaa acttcagcgt ggacatatcc aagtaagaca tgattagctg
     7201 catcctcata gccaagtagg aaatgatggg aatcctgaga cagtctagct agttggctag
     7261 ccgtttcctc tggactaaaa gtataaccca aagcctcttg gttgatgtca catatcgctt
     7321 tcacatctgt ttcttgcaaa tctcttagca tttcaagcct cctcaaaaga aatctctggc
     7381 aaccgagcaa gaatatcttc tcgtttaatt gcgccttggc gtaagatttt caccttgtct
     7441 ccagacaaat ccacaatagt tgaatcctgt ccagttagaa aagcatcgtc ttccagaccc
     7501 agaacctctt ggtcaaaatc cttcagaatt tgttcaaagg ttacaccact tgcctgacct
     7561 gagatattgg cagacggccc aatcaaggga cctgtctctc gaattaaatc cagtgtgata
     7621 gggtgactgg gcatccgaaa tccaatagtt gcaaggtcag aatttaccca atagggaact
     7681 cggtcattgg cttcgagaat aatggtcaag ggacctggca aaaaggtctc tacaagtttt
     7741 tgtagataag ttggctgatt cttagaaaag tgcaagatat cctcgaaaga ggcgatattg
     7801 agattgagtg ccttatctct aggacgacgt ttgagttggt aaacatggtc aactgctttt
     7861 tcatctaagg ccttggcaaa aagaccataa acagtctctg taggtagaac gacagctcca
     7921 ccattttcca actcttgtct aatcctgtcc atcatcaacc acaaccatcc tattttgacc
     7981 aaattggtct ttgagtgttc gaactttttt ttcaggaaga tgttttctaa aaagttcggg
     8041 aacactttga ccttgcttgt atccaatttc aaggtaaatc ttaccaccat ccttgagata
     8101 gtcttttgca tcttccgcaa ttctacagta aatagctagg ccatcctcgt ctgcaaagag
     8161 agctagatga ggctccgaat gcaaaacatt caagcctacc tctgactcat cttcacgaga
     8221 gatatagggt ggattggata caattatatc atatttttca gaaatttctg caaaacaatc
     8281 agatttttta aaaaatatat taagattttg atcgctagca ttctctgatg caagctctaa
     8341 ggcctcttga gaaacatctg ctgctgtcac tgaccaatct ggtctatttt ttgctagagc
     8401 gagagcaata gctccactac ctgttccaat atccagaact gagagattcg tctcaagatt
     8461 ttcagttaga ataagctcca ctaactcctc tgtttctgga cgaggaatca aaacccgttc
     8521 atccaccttt aaatgcattc cataaaaatc tgcctgtcca atgatgtact gagctggctt
     8581 gtgagctgct agttgctggt aaatatcttc tacaaattgt ttttcttcct ctgttgtcac
     8641 ctcctgctgg agggcaaaaa taaagtctgt aaaagataga tttttcagac tacgatagac
     8701 aaaagagagg ctttccgctt cctctccttg tcttatcaac tcttcttcaa aatttgaaaa
     8761 taattgagct aatttcatta tttgtttaat tcttctagtt tttgtgtttg gtcataaagc
     8821 accaaggcat ccacaacttc gtccaattta ccagacaaaa tcgtatctag tttttggagg
     8881 gtcaagccga tacggtggtc tgtgacacgg ttttgaggga agttataagt tcggatccgt
     8941 tctgaacggt caccagtacc gattgtcgac ttacgctcag cgtcttgttc atcctgagca
     9001 atctgagcaa agtggtcagc gacgcgtgca cggataatct tcatagcctt ctcacggttc
     9061 ttctgctggg tacgttcttc ctgcatctca accttgatat tggttggcaa gtgaacgata
     9121 cgaacggcag tcgcaacctt attgacgttc tgtccaccag cacctgatgc gtggtaaata
     9181 tcaacacgaa gatcttttgg atcgatatcg tattctactt cttcgacttc aggcatgaca
     9241 agaactgtgg cagttgaggt atgaacacga ccttggcttt ctgtcacggg gacacgctgc
     9301 acacggtggg cacctgattc gtacttaagc ttagagtata cagactgacc tgaaaccata
     9361 gcaaccactt ctttaaaccc accgacacca ttcatagagg cttccatgac ttcaaagcgc
     9421 cagccttggg cttccgcata cttttggtac atagttagca aatctccagc gaaaagtgcc
     9481 gcttcgtctc caccagctgc tccacggatt tcaaggatga tattcttgtc atcgtttgga
     9541 tcctttggaa ggagcaaaat tttcagtttt tcttcatatt cttctttttc agccttggca
     9601 tctttgagtt cttgcttggc catttcttcc aagtccgcat ctccgcctga ttccttaatc
     9661 atctcttcgg catcgacgat attttgaagg acttgtttat actcacggta ggctattacg
     9721 gtatcacgat tggaagcttc ttcttttgaa agctccataa aacgcttggt gtctgaaacg
     9781 acatcagggt cactcagcaa ttctcctagt tcttcataac ggtcttctac agcttgtagt
     9841 tgatcataga tgttcatttt tcttcattct ccttattgat ttcaggggca aaataatgtt
     9901 tacggcaaac cgagatatag gtttcattac caccaatttg gatctgttct ccatcgtaaa
     9961 cgggcagtcc atcctgtgtt cgcaacacca tggtcgcctt tttcttgcaa tactgacaga
    10021 tggtcttgat ttcgtcaatc ttgtctgcta aaagcaagag atatttggaa ccttcgaaca
    10081 attcattgcg aaagtcattt ttcaagccaa aagccatgac gggtatgtct aactcgtcca
    10141 caacacgagc taggtcataa acatggtggc gtttgagaaa ctgggcttca tcgaccaaca
    10201 cacagtaagg tttttctggt aggcctcgga tatagccaaa gatatccgtt gtttcctcaa
    10261 tcgcaagggc agggcgtttc atgccaattc gactcgacac atagccaaca ccgtcacgcg
    10321 tatccagagc tgaagtcata atcacaacac cttttccttg ctcctcatag ttataggcca
    10381 ccttgagaat ctcgattgtc tttccagagt tcatggtccc ataacgataa tacaactgtg
    10441 ccatgtttct tgcttcacgt ccatttctaa atttttgcta cattctagta tatcataatt
    10501 ttcttaagct ttaaacggca aaatgtggta aaatggaaga aatcaaaact agtggaggaa
    10561 gctattatgc catttgtacg catcgattta tttgaaggac gcacgctcga gcaaaagaaa
    10621 gctcttgcta aggaagtaac ggaagcagtt gtccgcaaca ctggagcccc tcaatctgct
    10681 gtccatgtca tcatcaacga catgccagaa ggaacttact tcccacaagg ggaaatgcgt
    10741 actaaataag ctagcttaag cagaattgct taggcttttt caatctccaa gtagcattca
    10801 ttgaagaaat atcctaaatt tgttacaatt tgaaaagaaa cttggagaat ttccaagaaa
    10861 agagctatta attaaaggaa acattatgat tacacgtgaa tttgatacca tcgctgctat
    10921 ctctactcca ctaggtgaag gggctattgg tattgtccgc ctgagcggaa cagacagttt
    10981 tgctattgcg caaaagattt ttaaaggaaa agacttgaac aaggttgcca gccacactct
    11041 caactacggt cacattattg atcctctgac tggtaaagtc atggacgagg ttatggttgg
    11101 ggctatgaag tctccaaaga ccttcactcg tgaggatatt atcgagatta acacccacgg
    11161 tggtattgcg gtgaccaatg agattctcca gctagctatc cgtgaaggag ctcggttggc
    11221 agaacctggt gaatttacca aacgtgcctt tttaaacggt cgcgtagatt tgacacaggc
    11281 cgaggcggtg atggatatca tccgcgccaa gacagacaag gccatgaaca tcgcggtcaa
    11341 acaacttgat ggttcccttt ctgatcttat caataatatc cgtcaagaaa tccttaacac
    11401 acttgcccaa gttgaggtca atatcgacta tcctgagtat gacgatgttg aggaagccac
    11461 tactgctgtt gtccgagaga agacaatgga gtttgagcaa ttactaacca agctccttag
    11521 gacagcacgt cgtggtaaaa tccttcgtga aggaatttca acggctatca ttggacgtcc
    11581 caacgttggg aaatcaagcc ttctcaacaa cctcttgcgt gaggacaagg ctatcgtaac
    11641 cgatatcgct gggacaacac gagatgtcat cgaagagtac gtcaacatca atggtgtacc
    11701 tctcaaattg attgatacag ctggtattcg tgaaacggat gatatagtgg aacaaatcgg
    11761 tgttgagcgt tcgaaaaaag ccctcaagga agccaacttg gttctactag tgctaaatgc
    11821 cagtgaacca ctgactgcgc aagacagaca acttcttgaa attagccaag ataccaatcg
    11881 cattattcta cttaataaaa ccgacctgcc agaaacgatt gaaacttcga aactacctga
    11941 agacgttatc cgtatttcag tccttaaaaa ccaaaacatc gacaagattg aagagcgaat
    12001 caacaacctc ttctttgaaa atgctggctt gattgagcaa gatgctactt acttgtcaaa
    12061 cgcccgtcac atttccctga ttgaaaaagc agttgaaagc ctacaagccg ttaatcaagg
    12121 tcttgagctg gggatgccag ttgatttgct tcaagttgac ttgactcgta cttgggaaat
    12181 cctcggagaa atcactgggg atgctgctcc agatgaactc atcacccaac tctttagcca
    12241 attctgttta ggaaaataag aaaaatccat gatccttcat tcggtcatgg attttattgt
    12301 ctttattagt aatctggtct taagacccct gttacagttg ccttagttgc ttcgtagtcg
    12361 ccatctacga caaccttgat aatgcgtttg gcatcttctt ctggtgctgg aacaagaggt
    12421 agacgagtgg gtccagcttc aaatcccata tagttaagaa ttgccttaac tggagcagga
    12481 cttggataag agaagagagc attaacctta ggaatgaatt tacgctgaat tgctgcggct
    12541 ttcttcatat cgctttctgc aatggcagta aacatctcgt gcatttcatc cccatttgta
    12601 tgagaggcaa cagaaataac cccatccgcc ccaaggttca tggcatggaa agcatctcca
    12661 tcctcacctg tataaatcaa gaactcttca ggcttgtgct caatcaagta agccatatta
    12721 gccaagctag tacattcttt gacaccgata atatttggat ggtcagccaa gcgaagcatg
    12781 gtttctggag tcaattcgac aactacacgc cctggaatgt tatagataat aattggtagg
    12841 tcagaagcat ctgcaatagt cttaaagtgc tgatacatcc cttcttgaga aggtttgttg
    12901 tagtaaggaa caatagcaag cccagctgcg aaaccaccaa attccgctac ttctttgaca
    12961 aactcaatag agtcacgcgt atcattagta cctacacccg caatcaaagg aacgcgtcca
    13021 ttgacaacct tttgtacagc cgcaaacaac tccaactcct catcgtgggt caaagttgga
    13081 ctctcagcag tcgttcctgc gagaagaatt ccatccgtat gatgggccaa taaatgctca
    13141 atcaaggctg gaatagcatc aaagttaata gaaccatcct catgaaaggg ggtaataaag
    13201 gctgtaatga ttttacattc ttttaaatct tgataagaca tgaaaacacc tctctatttc
    13261 aaagaaggag ttcatctgaa ctcctaaacg atatgactat tttaattcaa atttcaattc
    13321 ggctgttgga cgaaccaatc cacgttcatg aagagtttca gcaatctgaa ctgagttcca
    13381 agcagcacct ttgagaaggt tatctgaaac aacccacatg tgaattcctt tttctgcatc
    13441 caagtcttta cggatacgac caacaaaggt atcacgcgaa ccaactgcat tgatagcttg
    13501 aggatagatt tgatgagcta catcatcttc aagaacagca cctgggaagg ctgcgatagc
    13561 tgcttttact tcttcgattg gagccacttc ttttgtttcg atataaacag actcagagtg
    13621 agctgacaag actggaatac gcacacatgt tgcagatact gcaatgctat catcttccat
    13681 aattttctta gtttccttgg tcatcttcat ctcttcgtac gtgtaatcat tatcagtgaa
    13741 aacatcaatt tgtggaagag cgttaaaggc gataggataa tgtttcttgt caccacctga
    13801 aggcaaaatt tccgcatgca aatcacatgg tttcacacca tcattcaaga cttcacgaag
    13861 ttcacgttgt gtctcaagaa ttgctcccat accagcacct gaaacggctt gataagttga
    13921 aacaatgata cggtccaagc cccatttttg gcgaaccggc tcaagagcca ccatcatttg
    13981 gattgttgaa caattagggc aggcaatgat tccgttgtgg gcatcaagtg catgagcatt
    14041 gacctctgga acaaccaaag gaacatctgg attttgacgg aaataagatg tattatccac
    14101 tactaccacg ccagctttta ctgcgtatgg tgcatactta gctgatgtag aactacctgc
    14161 tgaaaagaga gcaatatcaa ctccttcaaa agctgtttca gtcgtttctt caattgtaat
    14221 atcttgatct ttaaatttca atgacttacc tgctgaacgt gcagaagcaa ggtaacggat
    14281 tttatcgatt ggaagtgttg attcttccaa catttttatc atctgagcac cgacagcacc
    14341 tgtcgcgccg actacagcaa ctgtatatcc cataaataac ctctttcgga attttctaaa
    14401 aatttctata atagaagtat tatactactt tttccatgaa ttgcaaagct ttttcatcat
    14461 tttttggaaa ataaaaatcc cagtcgctag actagggact aagaggcatc ttcatgtgat
    14521 gagcaggttc acacaactca tcaaggtccg ctcctgcgtt atgacctcct tatgctcaat
    14581 agtagtccga agactaccta tcgactcatc gcatattcta tattactaga gatacttgat
    14641 tttgtcaata agaaattttt tcgatcgagt ataacaaaaa actctcgtat tcaagagttt
    14701 ccctatacat atcatgctaa ttgccgggat cgaaccggcg acctcatcct taccatggat
    14761 gcgctctgcc aactgagcta aatcagctta cttaagaagt atactatatt cagaaaaact
    14821 tgtcaagttt ccttagaaat tttccatagg aaaaagccag gtaagaacta cctagcttat
    14881 cttcttctat ttacttaaat caattcgacg accaatttta ccaataataa tcgccccaat
    14941 aatcaatgaa gcaaattcac cgattcctgt tgtaaaccaa gtaaggaaga atggtaattc
    15001 tgctacaata tttaactctg cagcgatagt aaacatggaa attgaaaaga ggattgaaaa
    15061 gaagaaatga tcttttcgaa tcaatccgtt aaagaggtaa tccttgctat attttgcaaa
    15121 aagccaaaca cctaaactga ggaatactaa ggttgaacca ccaccaacaa agacatctag
    15181 cagtccgaag ctaaagaaat tagcaatcat acaaccgatg gtaactccga tgatgtactt
    15241 aggattgtaa aaagccataa agttcatcat ttctgaaata cgaaactgat aagcaccata
    15301 gctgatggca tttaggggcg gtgtgacagt caaaacgacg tagatagcag tgacgattgc
    15361 aatatctgca atatcacgaa tagttaattt tttcatcttt tctcctttgg cggttgcccg
    15421 cgtgtaatat gcttggtgaa agaagctaag caccaagggt tgctttattc aaccctacta
    15481 gtatagcaca atagcctgct ttatgctata cttaacacat gaaaattcct attcaaaaat
    15541 tttttaacaa tgaaatctta gcctatctct tttttggcct tgcgacaact ctagtttcga
    15601 ttctctcacg actagtcatt tatcaactaa gtcatcagga actcctggct actgccctag
    15661 caaatattat cggtatccta tttgccttta tcacaaatga tacgattgta tttaagcaaa
    15721 aaaagaaaaa tagacttatc cgtttagtga aattttctct tgctcgcctt tctacttttc
    15781 tgttagactt gtttttaact tttctctttg taacccaatt tcctcacatt atagggcaat
    15841 tcgtaactga aaacattgat aagataaatg ctattgaaac aataattgca caaatattca
    15901 ttattatcat caattatata ttgagcaagg cctatatttt tagaaaataa tgctccattt
    15961 atgagccttt taattgcctt ctataatgat ttaggataca atgaaacacg aaacattgaa
    16021 aggaaaacag tatgttaaaa aatctaaaat cgttcttgct tcgaggaaat gttattgacc
    16081 ttgctgtcgg tgttgtaatt gcctctgctt ttggtgctat cgttacttca cttgtaaacg
    16141 acattatcac tcctcttatt ttaaatcccg ctttgaaagc tgctaaagtt gaacgtatcg
    16201 ctcaactttc ttggcatgga gtcggctatg gtaacttctt aagtgctatt atcaatttta
    16261 tctttgtggg taccgccctc ttctttatta tcaagggcat tgaaaaagca cagaaactga
    16321 ctggcataaa gaaagaaaaa actgccgaaa aaaaaccaac cgaattggaa gtccttcaag
    16381 aaataaaagc tctccttgag aaaaaataat cgattaaaag gatctagcgc aaagctaaat
    16441 ccttttttct ttactctttg ggtaacttgc ctgctttttc aagcattttt ttgatcagat
    16501 aaggcatctt aacattctcc cgcccttttt tctcaatcag ttttttcaca aattctggca
    16561 tttgtaaatc ttcagattca gctaaaatgt ttttcgtctc gtctgaagga actaactcat
    16621 caaaggtctc ttcttctggg agaaattcct taaactcttg attttcattg actctaactg
    16681 tattgaccaa agcatcaatc aaacgagaat ctgtatttgg cattggtgga cgatggtagt
    16741 ttaccccaaa ttcctggcat aagtcataac attctacatc attgtcaaac aagacctcaa
    16801 tatgctcact gatgaaacta ataggtacaa aaatataatg gtctggatgt tctgtctgtt
    16861 ctctgagata ctccaaaaca tctggtttaa tccatggaat accaatatca ctctcactct
    16921 gccaagtgtt ggtatattgt tctgaactca agcccagttt ttcagcgact agcttgctat
    16981 tttcgaaaat ttggtctata taaggatcac caaagtccaa ggcaaaaatg ggcacactat
    17041 gggctgaaaa gatgacttta aagctgtctt gctttacttc ttcttttaaa atcttagcaa
    17101 tttcatctgc ccaatagttt aagagcactt cttcctgata ccagtcctta atgaccaaaa
    17161 attgaatttg tttgctttct aaaaattttt catagcccat gacagagtag aaagaataat
    17221 ggggctccaa aatcaagcag atacattctt caacaccatc agtttccatc tgcttgatca
    17281 cgtctggaat aaagggacga gaaaatttgt tggcaaagta gataccgtac tcctccccta
    17341 aacgttcttt gactaaattg acctcctcac gtgtaatccg ctgcaaaggc gtcccaccga
    17401 tacgaatata gttgtcatag agagtctgga tttcgtggtc ttggggtcta actccacgac
    17461 gaatgtttgt aaagaattct gctactcctt caaaactaat ctcctctgga gatccaaagg
    17521 tcatcattaa tatggctttt ttcatgttat catccttatg atattatttt ctctatccat
    17581 tctatcatga aagaaaaaaa tagtaaacgt ttcctacgtc tactatttct tttgctcttt
    17641 tgttttttta tctcgtctta taattaggat gataaggaca agaagtaaga ggacgataat
    17701 aaggagtaga aaggctagaa tgtaaatcca atgactggga attccgtgat cgaaataccg
    17761 tgttcctact gcttccccac tttcatcctg tgctactttc accggtgcat ccgttatttt
    17821 agctaaaagg atatgtcgtc cattagttat gttaaggaaa catctcgctt gtacttggct
    17881 ttacttagca gatattgata gtaagcctgg tgttcctcat ccttggaact caaggttcga
    17941 taaatctccg tgtcataggc atccacttct aaaatcccaa aaatttcaag accacgttct
    18001 cgaccattgt agtagataga accgtagcga tgctggtcga agaattcctt atcagcaaat
    18061 ttcttaatct caccgaacat agcccctgaa gccatagagt gtccgtagat gatcgtatta
    18121 aaatcagtaa agttgggatt aaagcgatag tcgagaaaaa ttgatcccat tatcgtataa
    18181 tcgccaaagg cattgcggtc aaggtatttg acattatcct gactttgtag aagcggatag
    18241 tctaccttgg tcccatcaag gctaatccaa ccgcaaacat ctttgttcaa gtgctgtagg
    18301 gtttcaaaac ctgtgctcga agcatccttt tcaataggat tatagtgatc atagtcactt
    18361 gcttctacta ctctctgcca actaaaaaga gctacaaaaa cacagaagaa taccagaaat
    18421 acgtttttaa atcgcgctct tttttgaacc gtcatcagca ctcctttcta tgcgagattt
    18481 cttaaaagga aaggaggcgg gacaaaaccg cctcttttct attcaccctt agtcagtaaa
    18541 agggtctgct tcttgcattt tttttactct ctcttctacg acgtaggatc aagtagagga
    18601 caaggagtaa gaaaatcaag agcaagaaca atacaaacaa gataaggtac caaatccata
    18661 gtgggattga tgagaggaaa cgtccaagag acgagtcatc aaagacactg tatggaaatg
    18721 gttttgattt ttttgtatgg aaagtatttt tagggacggt gtctgtaatc tttgcgacta
    18781 cgatatgacg accattggtc acatcgagaa aacaggtact gagtaggatg atacgatcac
    18841 tcggtgagag tgagatatcc cgcttgtgca tggcgactga gagcaggtgg tctagatagg
    18901 cctgctggcg gtcctcaccc tgtattcctg gatcatagat gttaaagtca taggcatcaa
    18961 cctccaacat ctcaaagatc tcgagccctt tttccacacc attgtagtat atactaccgt
    19021 aacgatgctg gtcaaaaaat tcctgatcag caaacttagc cacatcacca aacatgaccc
    19081 cattttctac gtggtgcccg tagataatgg tattaaagtc ttcgaactta ggattatttc
    19141 gtgcatcgag aaagatagcg cctgtcgctg caaactcacc tttggagtcc ttgttgagat
    19201 acttttcatt gtccttggct tggactaagg gatagtcgat attggtgcca tagacattga
    19261 tccaaccgag gacttcggga ttgagttctt ggagtttgct gaagcctgaa aaactggcca
    19321 gctcatcctg ttgcgtcgtg acaggtcgat aagcttcgta ctcacttgac gaagctaagg
    19381 agtagacttg gttggaatcc caaagggcgt agaaaccaaa gagaaagatc aaagctgcaa
    19441 aaatgagcag caagtgattg atcaccccat ccacagcaag aacgagacgc ctagacagac
    19501 tcttttttgg tttctcacgc ttggttttca tcatctgccc ccagccgttt aggcttctct
    19561 acgtttttta gacaagacaa caaatccagc caaaccaaga ccaatcatca aaatgaatgg
    19621 aaggttatca atcaagagac cagtaggagt aacgtcgtcg attttgttag taactttgtt
    19681 gtcgtttttc ttttcaccta tgaggatatc tgttaatgta aaatctacat ttttttgtcc
    19741 ttgagatgtt tgttctgcac catttgtagt ataaattgat gaatctgtat aacctgctac
    19801 tccagtttca gttactgtat atttagaacc tgctggtagt tgttcaactg taaatgtttc
    19861 accatcttta agagtaaaat tgtactcaac tgtatctgct ggggtcacta ctgccaacac
    19921 ctctgactta gatccatgtt ttactataat attcgtaaca ggggtttctg ctgttttatt
    19981 tgtacttgga agttgaatct tgatatggaa ttggaaatct cttgttttat cccctgaagc
    20041 tccatcaact tttttagtaa ttgttaatgc tttcgcattg gggtctactt gactcggatc
    20101 atcaggatta ggctccttac ttgcgtcttt ggtatatgtg ttatcaaata aattataaac
    20161 gccttgttca cttggttcta ctttaggggc gttagattta ttattttcct taaagtatac
    20221 tgatgaaata tagacaccac catttacttt attcttaaca ataacgtgca tttcatatgt
    20281 acgtttgtcg tatctcatgg catcaattgt tttaccattt ttggtaatcg cctgccatcc
    20341 agtttttgct tccgctacat cataaacgta ttcaccagca tggccataag caactcccga
    20401 aaaaatgtct tctgttgctt tttgaatctt atcagcagtt gggtgatcat ttttattgta
    20461 ttctacattt ctatctggaa ttgcaactcc ggtttgatat ggcgcaccgt tagaagattg
    20521 tcctgttttt tctgtaaact taaatgtaaa tgtcgctgtt ggtgttgtta ttccctctgc
    20581 aatattcaac gttttattaa tcttcacaac agcattattt tcaccatttg ctttggttat
    20641 tgctgaatta tcatccgcaa aagtcgggac acctctgaat acggacaaag cggttatagc
    20701 tgcaatgctt gcagtgaata gctttttaaa gaatgttttt ttcatgatta tttcttttgt
    20761 ttttttattt tcttttttca ttatgatcat caaaagcctc gtcttctaat gactgttaca
    20821 actttgcctt gagtatcctg tatagggatg gttccaaaag cacgactgtc tacagccgtt
    20881 gttcgattgt ccccgaggac aaaaagttgt cctgctggga ctttcatcgg gaaggttgcc
    20941 ccttccttat agagcagcgt ttctttgtag atatcttgct cttgttgagg agaaccgttg
    21001 atgatgagac cattttcgtt tatatcgatt gtacttcctt cggttgctat gacacgcgcc
    21061 actctttcct tacctttata actatagact agcaaatcac caatcgaata gcgtttatcc
    21121 aacctataat agacgaccaa gtcgccatct tttaaggcgg gcttcatgcc atcgtcattg
    21181 tatcttaata atccaaagac gaaaaagtaa aggatggcta gtacaaatcc aaccatcaga
    21241 agtttactga ctaagtaaag gatatctccc cagacactcg gcggagactt atcccgattg
    21301 acttgtgtta ctggtttctt atgtttcttt ttaagcagca tcacttcctc ctattcacca
    21361 caaggtgacc aagtaagatg ataaagaaga tccctcctag aggaagaacg actccaagca
    21421 cgagagtgct actcaagaag tcaattcccg tttcaggtac gctgttttta ttattcgtga
    21481 ctgtcgcaga cttatttgca tcaagagttc cttctttttt atcataggac acctgatacc
    21541 cacgagacga acttgcttct tcttcgatac tataacgagc atttagagga aggtcgagaa
    21601 tcttgatggt tttatctttc tttagatcaa ctgtcgcctt accattggtg aattgtagcg
    21661 ttgttttttg attatttact attgcactat acgatccatt caatggttta ttttgcgcat
    21721 cctttacgtt aatggtaatc ttaaaggatt gcagaagatt agcaaaagca cctgtaactt
    21781 tttttgtgac tgaaagtgtc ggaagtctgt ggttggtgac gattgtttga acatcctctg
    21841 acaaagtccc ctcttgtttt tcataggtga catgatatcc cgttctagag ttctctactt
    21901 cttccacctt atagtgggta tcacgaggca gataactgag ggcagtttcc tgactatttt
    21961 tgagagaaat ctgtgctttc ccgttagtca ctgttgtatt cccaaacttg ccgttaatcg
    22021 gcttgccatc cttatctgaa aaggtaacat taattgtgaa agatttatta ggtgattgtt
    22081 caccttctag gattttcttt acggtgagac ttggaagctg tcggtaaata actttgaagg
    22141 atggtttctc tttcgtacct gtcttttgaa cttcgactgt gtatcccgga gcgtctggtg
    22201 ctttcagttc gtagtcatat cccttagcta cctttgcaaa actagtattt tcagtaaaac
    22261 tattcgtttg aactggacta gtgacctttc gataggaatc ctttatactt ccatctttct
    22321 tttggtttaa gtttaattcg acactaggtg gacgatttgc tgatggattt gattttccat
    22381 ccacaccttt ccactcaatc tctacaggaa ccgtcaatgg atctgacggc ttgaaagtgg
    22441 gcttctcaga atagtctttt attttggtac gtgaggtccc aataccatag gaataggcaa
    22501 cttttgcccc cttgttggag tagagtttag tagcatcggc ttcttcggca tcatcactag
    22561 taagtgtctt atcacccgca atcgcatcaa atgcctcttg gctagaggtg acagtaaact
    22621 tgagaacgta cttggcgtca tcctccaaag tgtaactcgg attaaacttg gctgttactt
    22681 ccactaatcc ctgttcgttc ttcttagttt caatcgtaac ttgattgtca ttcagttctt
    22741 gctcgttacc atccttgatt ttgacgacac gaaggtgtga agcatcacca ggtaacagct
    22801 gcacgtactt ggacaagaca tctgatatag ttacatggtg tacgcctaga ggtagaattt
    22861 tatctgtgat ttctttgaat ttttgttgca agtcattttc attattggca gaaaatattt
    22921 cgttggggat tccagaatta taaccactga tatatccttt aagagatgtg atactatcgt
    22981 ttgaacctat atatctgaat ttgattgaat aaaagccatc tatttttgga gctattaaac
    23041 tagatatatt cagcattcct ctctctaaac cagaggtaaa ccaatatgga gtatcaatca
    23101 tacgtcccac atttggatta gaataatagt tatatattgt tctcccacta ttagactcgt
    23161 aatacatatt agcttcgcca tctgacagca gaataactac tttttttgcc ccagcccttg
    23221 cactttgcaa caatctttga gcttcagtta acccagcatt tatatttgtc ccagtgccaa
    23281 tgcccgcgtc cataccataa gaacgactag agtcagcaat tgttaacgaa ctaacaatat
    23341 tttttgagct attagaatcc gtgctccaag atactaaagt tttagcatca tcatatgaag
    23401 agatatttga ataccaacca gctcaatttg gccaagttcc atattgataa ctcagataat
    23461 attcattata ataacggtca ttatactgat tatcaatctt tccggcaaaa ccaactatag
    23521 acagacggtt ttgggaatta gaaagaattg tatcaatgag cccctgacga ccattcacac
    23581 ctttcagtgt atgttttagt gcttcaatcc tactaatgta tcgtctacta gagtaagacc
    23641 acacatctcg ctcttccata ctccctgaaa gatcggcaac caagactaca tccaagggtt
    23701 ctgactgggt ctccgttccc agtttagatg tgatatccag tgacaactca tactggtctt
    23761 tttgccctga tatcggagta atcgttttgt gcagagttgt ctgaggttca gttattgaat
    23821 ctgcacgtaa agaattaatt ggtgcacaca aaaaaagaat agcgagaaag ataactaaac
    23881 tactggtaaa atattgaatc ttcttaaaat catattgaac gttcatgcta tctcccttgc
    23941 tagaatatgt tttaatctga tactatataa atctgagact tccataagat aattgtataa
    24001 taatattaac ttcgtgtcaa ttaatgtgtt tgaaaacatg ttattataaa ttacaaaaaa
    24061 gtatgattct tctttgtttt tcaacttttt ctgtggtaaa atggaaaaaa tagtaaaaaa
    24121 tttcataaaa taaagatgca tatatttatt ctaatcgctc gaaaatttct tttgaaagtc
    24181 ttctgaattt gaattattat attttttcaa aattcataaa atttctcaaa tcatattttt
    24241 ccgaagtttt gtattctatt ttatgataaa aaaggagttt ttttatgact tatccaaatc
    24301 tcttggaccg cttcttaacc tacgttaagg tcaacacgcg ctctgatgaa cactctacta
    24361 ctactccaag tacacagagt caggttgact tcgcaacaaa tgtcctaatt cctgaaatga
    24421 aacgtgttgg actgcaaaat gtttactatc taccgaatgg ttttgctatt ggaaccttgc
    24481 cagccaacga tccgtcttta acacgtaaga ttggttttat atcgcacatg gatactgctg
    24541 attttaatgc tgaaggagtc aatccacagg taattgaaaa ctacgatggt ggtgtgattg
    24601 aactagggaa ttctggtttc aaactcgatc cagccgactt caagagtctt gaaaaatatc
    24661 caagacaaac actcatcaca acagatggaa caaccttgct aggtgctgat gacaagtcag
    24721 gaattgctga aattatgaca gccattgaat atctaactgc tcatcctgaa attaagcact
    24781 gtgagattcg tgttggtttt ggtccagatg aagaaatcgg tgttggtgcc aataaatttg
    24841 atgcagaaga ttttgatgtg gattttgcct acactgttga tggtggtcca ctaggtgaac
    24901 ttcagtacga gactttctca gccgctggtg ctgaattgca tttccaaggc cgtaatgtcc
    24961 accctggtac tgccaaaggg cagatggtca atgcccttca gctagcaatt gattttcata
    25021 atcaacttcc agaaaatgac cgacctgagt taactgaagg ttaccaaggt ttttaccatc
    25081 taatggatgt gacaggtagt gttgaggagg tgcgtgcaag ctacatcatt cgtgattttg
    25141 aaaaagatgc ctttgaagcg cgtaaagcat ccatgcaatc tatcgctgat aagatgaatg
    25201 aagaacttgg gagcgaccgt gtcactctca acttgacaga ccagtactac aatatgaaag
    25261 aagtcattga aaaagatatg actccaatta ccattgctaa agccgttatg gaagatctag
    25321 gtatcacgcc tattatcgaa ccaatccggg gtggaacaga cggctctaag atttccttta
    25381 tgggaatccc aactccgaat atctttgcag gtggcgaaaa tatgcacgga cgttttgaat
    25441 acgttagcct tcagactatg gaacgtgcag ttgataccat cattggcatc gtagcttata
    25501 aaggctaaaa agacgaggtg gctcagctac ttcgcctttc tttttattct actggttttt
    25561 cttgatttcc agtagttgta gaagattctg ttgtttcatt ttctgaagtt gattcagcag
    25621 gtttagaatc tcttgtattg cttggtttgt tttcgtcgct agcagtttca atgttagatt
    25681 ctgcagttgc gtttggttgg ttctcagcac tggtgttatc actatttgct tcagcatttc
    25741 ttgctggact tgtttcttca cttgcgctaa cttttgactg gatttgatga ttcaaaacta
    25801 gaatagcttt tgtcgattca agtaaagctg ttttgtcttt actattagca gaaagttgat
    25861 ctaataatgc atccacctta tcaaagtccg catcagatcc attattactt tctaaataag
    25921 agtgaagcga catgagaata tcgtagagtt tttgatagag tacaagtgtc tgagaatctt
    25981 gctcagcatt ttccttttct tgttgaaggg cgctagcgat acgagtcaaa acatctttta
    26041 cctgactgtt tacttcatcc aagtctgcat cagccttgtt tgtggcagct tttagatttt
    26101 ctacttcttc tgccaaggat tgtctgattc cttcttcatg gatttgttcc aagagttgat
    26161 ttgccttgtt caaaagactt tctacttctt ccttgctatc tgtcgcagat tattggttgc
    26221 tatctaccat gtactcctaa aacaggagag ttataatcca agattacaag gccttacaga
    26281 aataagaaat ccagataaga caatgttcgt ccaagacgct attcgcttcg cacagcagca
    26341 cggattcaat atgctttaat tttaaagttt aggtgtcaag acctcttttt agtgtgccca
    26401 aaatttagag aagtaatcaa tcaactaact tttatttttt tcaaactttc agtaaactga
    26461 cctaaagcta actcaatctg tctttgtaga tgcttctgct atcagctaga agttgatcta
    26521 cttttgccaa gactgccttc tcatcaaaag ttccaggttg atagttggat tgcagggatg
    26581 gaatcttgtt tttcaaagcc gcttcatatc ccttagtttg aaccttgatg tagtgattgt
    26641 ggtcgccatg aggaatcaca aaaccttctg aatcttcact tataattcga ttggcatcaa
    26701 aaaccataac catcttcttc ctcatggtgg acatgtagtg acggattact taatacagaa
    26761 ctagaagaac ttcctacctc ttccgtgtta gagtgtgatg ggggattgtt aagagatgac
    26821 ttaggaatat agtgatagtg ttccccatgt cttactatat aagcatcacc tatatctctg
    26881 acaatatcat tagggttaaa gacatatgtg gctgctaatt cacctgccga caagtcactc
    26941 tcaggaatga aatgatagtg accaccatgt ggtactatta gtagattgaa atagaatatg
    27001 agcaaattga taaggggatt ttaaagtaat ttctaacaat gatttagaaa ctatgatgtg
    27061 ctattctaaa ttcaactcac tatatataac catcatcggt agtataacgt ccctgtaatt
    27121 ttgctacaga tacttctgca ctagctcctt tatcgtcttt accatgttct tgtttttggc
    27181 gattgatttc atcttttgtt cgtacatttt ctgcatgagc ttgatcttta aggtaaacat
    27241 aatactttcc atctacctta ataatatatc ctcccttaac ctaactgaca atatcttgat
    27301 ctttcggctg atagttgggg gctttcatta atggctcttc actaaagagc gcatcaaaag
    27361 gaactttacc attatagtag tgataatgat cgccatgaga agttacataa ccttgatctg
    27421 taatcttaat aacaatttgt tttgcttgaa ttccttcttt ttgactaacc tagtctggag
    27481 tcaaattttc agtcttctta gtgtctttat tactgtttac atatgaaaca cgatttttat
    27541 ctgtattggc ctgttagcta tgttggttca gagcataaac acacagactt aaggaaagga
    27601 taacaacaga tccagctgct atatatttct ttttaaattt cataattacc tcatttctat
    27661 aattatttat atgatgtctt cattattaaa tgattataga aattaattaa ccaattaatt
    27721 aactagtaaa tattccacct ctttttaagt tgtatgtcaa gaaattttat atattaataa
    27781 taaaatgaaa ttctcccaaa gtcagagttt tatttctaac ttttgagaga acttcatttt
    27841 tgattcagac tttttctact gctattcctt acgctatgag atcagataaa ttctttttta
    27901 tcacttctcc acttggcaat cttaattcaa tcgttccatc catattgaat ataacactat
    27961 ctaagcctaa tccgtaacta gctgtaaatt tttctaattt ttcttgtaca ggatctactg
    28021 ctggagcttc ctctaatgct ggatctaaca tagggtcact ccccacattc ccttctggat
    28081 tcaacattcc attatccgtt gagttttctg gttttacagg tttttcgttt ggtgcctctg
    28141 gtaaagaatc tgctggttta ttttctgttg gttggttctc aactgttcca gtagatactt
    28201 ttccattttc agatggttta ttttcaccat ttccttgagg tgcttctcct gtaaaatctg
    28261 ccatattctt tttaatgact tctcccgatg gtaaatataa ttcaattgtt ccgtccatat
    28321 taaacaagac attttctagc ttcatcccat aactttcagc aaattttgct actttttctt
    28381 gtacaggatc cactgtagga acttcttcta acgttgaatt actagtacta ttcccagttt
    28441 cagaaagttt ttctttttct accttctcac tagtctttgg ttcttctacc ttttcatcaa
    28501 gttttgagtt ttcttgtgct ttattccttt taaattgtgg tagaatactt ggtttatcag
    28561 tttgattttc tttttccaag ataggtactt ccacaatata agtcgattga ttgtccaaat
    28621 aagcatttgc catgaaggtt acaggaattt tatttccggc cgttctggtt gttccttggt
    28681 ttaatttcgg aatcggtaat ttgatttcac caactttata gttattttct aaataagcat
    28741 ttccatgaaa ttcatcaaac actctgacta aagcatcagt tcctttaggc actgcaaatt
    28801 gagggttcac tcttaaataa gtatcccctg catggaaagg atagaaaatc gtttgactgg
    28861 ccattttgta agctaaagag gttggaactg taaatgtacc atcataactt acttctggat
    28921 aatcttttga agcgatagta tacttaaatg tttgtcctgg taaataaggt tgatctaatt
    28981 caaagtttgc aatattccct actccttctc caaatacttt accagatact ttctccaata
    29041 cttttccatc tggtgttatt aattttacta gcatattgat acctaatttt ttctccaatt
    29101 caggcggaaa actaaaagaa acgcgttttt gaccattggc tagagtaaag ttttgattat
    29161 taaacgtact attttttaac aaattaacaa cattcgttaa ttcttctcca gtataaactt
    29221 tattcccttc ttttttagca actccttctt cgggtttaaa cagttcatag ttactgtgag
    29281 aatgaccaat tccaaccggt ttatgttcat caatcggatc tgcatgatgg tgatctccat
    29341 gcggataaat aatcgcattt ttttctttat tcacgacaat actttcacgt ttgacaccat
    29401 attgtttcat aatgccagca attttttctt cgattttttt atctaaatct ttcatttctt
    29461 tggcattact tggataatcc tgttcatgag atgacaaaga atctaatcca ttatgactag
    29521 ttttaacttc ctctaaatgt ttttgcgcag ccttaatttg ctcttctgtc aagtccttct
    29581 tgaagaaata atgattgtgg tctccgtgac tcatgacaaa acctgattca tcttcagcga
    29641 taatacgatt agcatcaaat ccgtatccat cttcttcatg tttctcatgt gaagttcctg
    29701 gattgattgg aagagatgga gaaggtgttg ctagactatt gtttggaaga gtcggttgcc
    29761 caatttgatt tgattttgga atgtaatgga aatgatcacc atgtcttaca atataagctg
    29821 tagccgtttc ttcaacgata tcttttggat taaaaatata accatcagat gctgaagaga
    29881 gctccttact tgtcgttaaa gaagaaggat tgcttgaaag actgcctaga ctagacacta
    29941 cttcattagg ttttgcattt gtagaaactg tagaaccagt tccactgata ggcaccattc
    30001 tggcaatctt ttcttctaag gcagaaagct tgctgtaagg aataaagtgg taatggtcgc
    30061 catgcggaat cgcaactcca tttggtgtac gactgataat cttagcaggg tcaaagacca
    30121 ggccatctga ttcactgtaa cgttgggcgc taggtgaatc atagagttcc ttcaaaagac
    30181 tctggagatt ttcagattta tttgctggct tgctagttga tccttttgct acagattgcg
    30241 tgttattgtc actagctgtt gaagaatagc ttaactgact cggttgcata ttttttccag
    30301 ccagatgtgc tttagctgct gctaattcac tagcagataa atcgcttttg ggaatgtagt
    30361 gatagtgacc tccatgagga acgatataag cattacccgt atcttcgata atatcagctg
    30421 gattaaagac ataaccatca tttgtcgtat atcgtccctg agaccttgct acagcaacat
    30481 tagagttaac cttctcatta tctttgacat gttcttgttt ttgacgattg atttcatctt
    30541 tagttcgaac attatcagca tgagctgcat ctttcaggta gacataatat tttccatcga
    30601 ccttgatgat ataaccaccc ttgacttcat tgacaatatc agcgtcttta agttgatagt
    30661 ttggatcctt catcaagagt tcttcactaa agagggcatc ataaggaact ttcccattat
    30721 agtaatgata gtggtcaccg tgtgacgtta catagccctg atctgtaatt ttgattacaa
    30781 tttgctcggc ctgaattcct tctttctggc taatctggtc tggtgtcaag ttttcacttt
    30841 tctgacttga ctggctgcca tctacataag agacacgatt attgtcctta ttttcctgcg
    30901 aacgatgctg gtttagtgca taggcacata gactcaagga tacgataaca gctgatccag
    30961 ctgctatata ttttttactg aatttcataa atccctcatt tca
//
LOCUS       DAVBCN010000018        30552 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVBCN010000018 DAVBCN010000000
VERSION     DAVBCN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30552
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.018"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(<1..916)
                     /gene="cysK"
                     /locus_tag="VQ497_000753"
     CDS             complement(<1..916)
                     /gene="cysK"
                     /locus_tag="VQ497_000753"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET2517093.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYE"
     gene            complement(1201..1836)
                     /locus_tag="VQ497_000754"
     CDS             complement(1201..1836)
                     /locus_tag="VQ497_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HET2517094.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITTIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            1892..3190
                     /locus_tag="VQ497_000755"
     CDS             1892..3190
                     /locus_tag="VQ497_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2517095.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            3187..3849
                     /locus_tag="VQ497_000756"
     CDS             3187..3849
                     /locus_tag="VQ497_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET2517096.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQFWCKEGVGVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            3929..4477
                     /gene="raiA"
                     /locus_tag="VQ497_000757"
     CDS             3929..4477
                     /gene="raiA"
                     /locus_tag="VQ497_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET2517097.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            4613..6592
                     /locus_tag="VQ497_000758"
     CDS             4613..6592
                     /locus_tag="VQ497_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET2517098.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTSLLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDV
                     SREQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMF
                     SRRVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDE
                     IGKVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISD
                     KIRSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFI
                     EKEGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQD
                     FPDNTVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRV
                     TSRTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTK
                     CYDTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGH
                     FNLAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            6589..7041
                     /gene="rplI"
                     /locus_tag="VQ497_000759"
     CDS             6589..7041
                     /gene="rplI"
                     /locus_tag="VQ497_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET2517099.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            7085..8437
                     /gene="dnaB"
                     /locus_tag="VQ497_000760"
     CDS             7085..8437
                     /gene="dnaB"
                     /locus_tag="VQ497_000760"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET2517100.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            8439..8705
                     /locus_tag="VQ497_000761"
     CDS             8439..8705
                     /locus_tag="VQ497_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517101.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            8794..10140
                     /gene="cbpD"
                     /locus_tag="VQ497_000762"
     CDS             8794..10140
                     /gene="cbpD"
                     /locus_tag="VQ497_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HET2517102.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSGTVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      10223..10325
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10396..10686
                     /locus_tag="VQ497_000763"
     CDS             10396..10686
                     /locus_tag="VQ497_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HET2517103.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            10649..11407
                     /locus_tag="VQ497_000764"
     CDS             10649..11407
                     /locus_tag="VQ497_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2517104.1"
                     /translation="MRNLKSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     NPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            11445..12452
                     /locus_tag="VQ497_000765"
     CDS             11445..12452
                     /locus_tag="VQ497_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2517105.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            12452..13180
                     /locus_tag="VQ497_000766"
     CDS             12452..13180
                     /locus_tag="VQ497_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2517106.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            13299..13757
                     /locus_tag="VQ497_000767"
     CDS             13299..13757
                     /locus_tag="VQ497_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HET2517107.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            13759..16191
                     /locus_tag="VQ497_000768"
     CDS             13759..16191
                     /locus_tag="VQ497_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2517108.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFMELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALVLGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     ALRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKVKYVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            16833..17486
                     /gene="rr06"
                     /locus_tag="VQ497_000769"
     CDS             16833..17486
                     /gene="rr06"
                     /locus_tag="VQ497_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HET2517109.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            17483..18823
                     /gene="hk06"
                     /locus_tag="VQ497_000770"
     CDS             17483..18823
                     /gene="hk06"
                     /locus_tag="VQ497_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HET2517110.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILFLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            18906..19433
                     /locus_tag="VQ497_000771"
     CDS             18906..19433
                     /locus_tag="VQ497_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HET2517111.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            19512..21047
                     /gene="pspC"
                     /locus_tag="VQ497_000772"
     CDS             19512..21047
                     /gene="pspC"
                     /locus_tag="VQ497_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HET2517112.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEGVRSGD
                     TPKVTSSGDEVESHLKKILSEIQTQLDRKRHAKTVALINKLQDIKRTYSYKLNVLEEK
                     PKAELTSKTKEELTAAFEQFKKDTLPTEPGKKVAEAEKKVAEAEKKAEDQKEEDRRNY
                     PTNTYKTLELEIAEFDVKVKEAELELLKEKAKKPLNEGTIKQAKEKVESKKAEATRLE
                     NIKTDRKKAEEEAKRKAAEEDKVKEKPAEQPQPAPAPQPEKPAPKPEKPAPAPKPEKP
                     APAPKPENPAEQPKAEKPADQQAEEDYARRSEEEYNRLTQQQPPKTEKPAQPSTPKTG
                     WKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNGAMATGWLQNNGSWYYLNANGSMA
                     TGWLQNNGSWYYLNANGSMATGWLQYNGSWYYLNANGDMATGWLQYNGSWYYLNANGD
                     MATDWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGSGALAVNTTVDSYRVNANGE
                     WVN"
     gene            complement(21277..22257)
                     /gene="dusB"
                     /locus_tag="VQ497_000773"
     CDS             complement(21277..22257)
                     /gene="dusB"
                     /locus_tag="VQ497_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET2517113.1"
                     /translation="MTNLNTPFMIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(22244..23116)
                     /gene="hslO"
                     /locus_tag="VQ497_000774"
     CDS             complement(22244..23116)
                     /gene="hslO"
                     /locus_tag="VQ497_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HET2517114.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQERHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGAGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            23281..24708
                     /locus_tag="VQ497_000775"
     CDS             23281..24708
                     /locus_tag="VQ497_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2517115.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLDSAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            24867..26375
                     /gene="glpK"
                     /locus_tag="VQ497_000776"
     CDS             24867..26375
                     /gene="glpK"
                     /locus_tag="VQ497_000776"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET2517116.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            26417..28243
                     /gene="glpO"
                     /locus_tag="VQ497_000777"
     CDS             26417..28243
                     /gene="glpO"
                     /locus_tag="VQ497_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HET2517117.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPILDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            28313..29017
                     /locus_tag="VQ497_000778"
     CDS             28313..29017
                     /locus_tag="VQ497_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET2517118.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            29314..29517
                     /locus_tag="VQ497_000779"
     CDS             29314..29517
                     /locus_tag="VQ497_000779"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517119.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(29604..30191)
                     /locus_tag="VQ497_000780"
     CDS             complement(29604..30191)
                     /locus_tag="VQ497_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517120.1"
                     /translation="MFVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNILSDEVLE
                     VVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVNTIHGNKQ
                     ANTFANFMTGAGISYMIGRIGTLLGGPWGAIIGGAYFGIQAYQSYLDSQSPYPYYITS
                     TYIHVAQKKWKFITEYYRNSNYTGYVKTVTTYVNF"
BASE COUNT         9346 a         5382 c         6936 g         8888 t
ORIGIN      
        1 attcataaag tgctgtagag agataacgtt caccgttatc tggtgctagg gcaaggactt
       61 ttttacctgt acctaatttt ttggcaacct cgatggctcc gtagatagct gcagctgagg
      121 aaatccctac aaggaagcct tcttttccac caatttcacg tccgagtgca agagcgtcat
      181 ctgatgttac acgaacgata ccatcatagg ctttagtatc aagtgtatca ggaataaatc
      241 cagctgagat accttgaatt ttgtgaggac caggtttttc accagataga atagcagatt
      301 catctgcttc tactgcaaaa acttgaatgt tagaattttc tgatttgagt gcatgagaaa
      361 caccagaaat cgttccacca gtacctactc cagcaacaaa ggcatctaat ccatctttac
      421 cgaaagcagc tagtatctca gctcctgttg ttctttcgtg tacttctgga ttagctggat
      481 tgtcaaattg aagaggaagg aaaccatcac gttcagcagc gatttcttga gccttagcaa
      541 tagcaccttt cattccctcg ctaccaggag ttaggacgag ttcagcacca taagcttgga
      601 taattttacg tcgttctaca ctcatagttt caggcataac gatgacgact ttataccctt
      661 tagcagcacc tacccatgaa agtccaatac cggtgtttcc acttgttgct tcaacaatag
      721 tagaaccaag tttcagaata ccatcttgtt cagctttttc aatcatgcta agggcaatac
      781 ggtcttttac agatgaacca ggattaaatg cttcaagctt tatatagacg tctgcagcac
      841 cttctggcac gatgttgtta agtttaacaa tcggtgtttg accgattaat tcagtaatgt
      901 tgttataaat agacatatga atacctcttt gtataagata tctttagtat aacgctatcc
      961 ataccatttg taaaatatag aaatcctatc gaagcgatag gatttctata tatcatagat
     1021 caaagtcatt aattttcaag tgattgcgtt cagttaattc tagtaaatag gtgtgaagga
     1081 aaacgatatc aggtttcgtt tgaattgctt cattttctag tatattttgg tacgcatctt
     1141 tttattttgc tcgctaaagt agttgtgaga atgtcaacga aacgctgtat tattcattgt
     1201 ttacactaag tttacaggaa cttcaacctc tcgtaaacct tggtcagtta aagtgacttt
     1261 tccattaaaa aactccacaa gtgcagcttt aatagtttct ttttcttctt tatcaacata
     1321 aatcatcgta tcaacttgat ctgtaaagtt tgtatccagc tccatgagat catgttcttt
     1381 aaggaagtta ctgtactctt ggtactgagc ataagacatt tgaatagcaa tgccagcctg
     1441 ttcttttatt tcaataatac caatttcttt gacagctaag gcgacactgc cggcgtaagc
     1501 acgaattagt cctccagcgc ctagtttaat accaccaaag tagcgtgtca cgaccacaca
     1561 gacattggtg agattgtgat tttctagtac cccaagcatg ggaacaccag cagtaccact
     1621 aggctcacca tcatcacttg tacgtttaat ttcactacgt tctccaataa taaaggcaga
     1681 gcagttatgt gtcgctttgt agtgttcttt tttgatggta gtaatgaagt cacgagcctc
     1741 ttcttcgcta taaacacgct tggcatggca gataaagcga gattttttga tttcttcttg
     1801 gacttggccg tcctctttaa ttgtcctaaa ttccatgatt taattgtact aaaaaatcat
     1861 ctaaagtgct agtttttacg aataaagaag tatgaaagta aatttagatt atctcggtcg
     1921 tttatttact gagaatgaat taacagaaga agaacgtcag ttggcggaga aacttccagc
     1981 aatgagaaag gagaagggga aacttttctg tcaacgttgt aatagtacta ttctagaaga
     2041 atggtatttg cccatcggtg cttactattg tcgagagtgc ttgctgatga agcgagtcag
     2101 aagtgatcaa actttatact attttccgca ggaggatttt ccgaagcaag atgttctcaa
     2161 atggcgcggc caattaactc cttttcaaga gaaggtgtca gagggactgc ttcaagcagt
     2221 agacaagcaa aagccaacct tagttcatgc ggtaacagga gctggaaaga cagaaatgat
     2281 ttatcaagta gtggctaaag tgatcaatgc gggtggtgca gtgtgtttgg ctagtcctcg
     2341 catagatgtt tgtttggagc tgtacaagcg cctgcaacag gatttttctt gcgggatagc
     2401 tttgctacat ggagaatcgg aaccttattt tcgaacacca ctagttgttg caacaaccca
     2461 tcagttattg aagttttatc aagcttttga tttgctgata gtggatgaag tagatgcttt
     2521 tccttatgtt gataatccaa tgctttacca cgctgtcaag aatagtgtaa aggagaatgg
     2581 attgagaatc tttttaacag cgacttcgac caatgagtta gataaaaagg tccgtttagg
     2641 agaactaaaa agactgaatt taccgagacg gtttcatgga aatccgttga ttattccaaa
     2701 accaatttgg ttatcggatt ttaatcgcta cttagacaag aatcgtttgt caccaaagtt
     2761 aaagtcctat attgagaagc agagaaagac agcttatccg ttactcattt ttgcttcaga
     2821 aattaagaaa ggggagcagt tagaagaaat cttacaggag caatttccaa atgagaaaat
     2881 tggctttgta tcttctgtaa cagaggatcg attagagcaa gtacaagctt ttcgagatgg
     2941 agaactgaca atacttatca gtacgacaat cttggagcgt ggagttacct tcccttgtgt
     3001 ggatgttttc gtagtagaag ccaatcatcg tttgtttacc aagtctagtt tgattcagat
     3061 tggtggacga gttggacgaa gcatggatag accgacagga gatttgcttt tcttccatga
     3121 tgggttaaat gcttcaatca agaaggcgat taaggaaatt cagatgatga ataaggaggc
     3181 tggtctatga agtgcttgtt atgtgggcag actatgaaga ctgttttaac ttttagtagt
     3241 ctcttacttc tgaggaatga tgactcttgt ctttgttcag actgtgattc tacttttgaa
     3301 agaattgggg aagagaactg tccaaattgt atgaaaacag agttgtcaac aaagtgtcaa
     3361 gattgtcaat tttggtgtaa agaaggagtt ggagtcagtc atagagcgat ttttacttac
     3421 aatcaagcta tgaaggattt tttcagtcgg tataagtttg atggagactt cctgttaaga
     3481 aaagttttcg cttcattttt aagtgaggag ttgaaaaagt acaaagagta tcaatttgtt
     3541 gtaattcccc taagtcctga tagatatgct aatagaggat ttaatcaggt tgagggcttg
     3601 gtagaggcag caggctttga gtatctggat ttattagaga aaagagaaga gagagccagt
     3661 tcttctaaaa atcgttcaga gcgcttgggg acagaacttc ctttctttat taaaagtgga
     3721 gtcactattc ctaaaaaaat cctacttata gatgatatct atactacagg agcaactata
     3781 aatcgtgtta agaaactgtt ggaagaagct ggtgctaagg atgtaaaaac attttccctt
     3841 gtaagatgag gaaaaaattt gcaaaaatag aatgaaagcg ttataataaa atcataaaaa
     3901 caaaaatgtt tagaaagaag gtactcatat gattaaatat agtatccgtg gtgaaaacct
     3961 agaagtaaca gaagcaattc gtgattatgt agtttctaaa ctcgaaaaga tcgaaaagta
     4021 cttccaacca gaacaagagt tggatgcccg aattaactta aaagtttatc gtgaaaaaac
     4081 ggctaaagtg gaagtaacga ttccgcttgg atctattact ctccgcgcag aagatgtatc
     4141 tcaagatatg tatggttcaa ttgaccttgt aactgataaa attgaacgtc agattcgtaa
     4201 aaataaaaca aaaatcgagc gtaaaaataa aaataaggta gcaactggtc aattatttac
     4261 agatgctttg gtggaagatt caaatgttgt ccagtctaaa gttgttcgtt caaaacaaat
     4321 tgatttaaaa ccaatggatt tggaagaagc aattctacaa atggatttat tggggcatga
     4381 tttctttatc tatgtggatg ttgaagatca gacaaccaat gtgatttatc gtcgtgagga
     4441 tggcgaaatt ggtttgttag aggttaaaga atcttaattt caatatgtgt aaaggtaggt
     4501 ttactgaatt gtaaactacc tttttcttat aattgataga attactgatt acacttttaa
     4561 aaagtcgctt tttgaggggt attatggtat aatgggtgcc aagaggtttt gaatgaaaaa
     4621 attttatgta agtccaattt ttcctattct agtaggattg attgcgtttg gagtcttatc
     4681 cactttcatt atttttgtta ataataatct gttgacggtt ttaattttgt ttctttttgt
     4741 aggaggctat gtttttttat ttaagaaact gagagtgcat tatacaagga gtgatgtaga
     4801 acagatacag tatgtaaacc accaagcgga agaaagtttg acatctctat tggaacaaat
     4861 gcctgtaggt gttatgaaat tgaatttatc ttctggagag gttgagtggt ttaatcccta
     4921 tgctgaattg attttgacca aggaagatgg tgattttgat ttagaagctg ttcaaacgat
     4981 tatcaaggct tcagcttcag taggaaatcc gtctacttat gccaagcttg gtgagaagcg
     5041 ttatgctgtt catatggatg cttcttccgg tgttttgtat tttgtagatg tatccaggga
     5101 acaagccata acagatgaat tggtaacaag tagaccagtg attgggattg tctctgtgga
     5161 taattatgat gatttggagg atgcaacttc tgagtcagat attagtcaaa tcaatagttt
     5221 tgtagctaat tttatatcag agttttcaga aaaatacatg atgttttctc gtcgggtaag
     5281 tatggatcga ttttatctat ttactgacta cacggtgctt gagggcttga tgaatgataa
     5341 attttctgtt attgatgctt tcagagaaga gtcgaaacag agacagttgc ccttgacctt
     5401 aagtatggga ttttcttatg gcgatggaaa tcatgatgag atagggaaag ttgctttgct
     5461 caatttgaac ttggctgaag tacgtggtgg cgatcaggtg gttgttaagg aaaacgacga
     5521 aacgaaaaat ccagtttatt ttggtggtgg gtctgctgct tcaatcaagc gtacacggac
     5581 tcgtacgcgc gctatgatga cagctatttc agataagatt cggagtgtag atcaggtttt
     5641 tgtagtcggt cacaaaaatt tagacatgga tgctttgggc tctgctgtag gtatgcagtt
     5701 gttcgccagc aatgtgattg aaaatagcta tgctctttat gatgaagaac aaatgtctcc
     5761 agatattgaa cgagctgttt cattcataga aaaagaagga gttacgaagt tgttgtctgt
     5821 taaggatgca atggggatgg tgaccaatcg ttctttgttg attctcgtag accattcaaa
     5881 gacagcctta acattatcaa aagaatttta tgatttattt acccaaacca ttgttattga
     5941 ccaccataga agggatcagg attttccaga taatacggtc attacttata tcgaaagtgg
     6001 tgcaagtagt gccagtgagt tggtaacgga attgattcag ttccagaatt ctaagaaaaa
     6061 tcgtttgagt cgtatgcaag caagtgtctt gatggctggt atgatgttgg atactaaaaa
     6121 tttcacctcg cgagtaacta gtcggacatt tgatgttgct agctatctca gaacgcgcgg
     6181 aagtgatagt attgctatcc aggaaatcgc tgcgacagat tttgaagaat atcgtgaggt
     6241 caatgaactt attttacagg ggcgtaaatt aggttcagat gtactaatag cagaggctaa
     6301 ggacacgaaa tgctatgata cagttgttat tagtaaggca gcagatgcca tgttagccat
     6361 gtcaggtatt gaagcgagtt ttgttcttgc gaagaataca caaggattta tctctatctc
     6421 agctcgaagt cgtagtaaac tgaatgtaca acggattatg gaagagttag gcggtggagg
     6481 ccactttaat ttggcagcag ctcaaattaa agatgtaacc ttgtcagaag caggtgaaaa
     6541 actgacagaa attgtattaa atgaaataaa ggaaaaggag aaagaagaat gaaagtaatc
     6601 tttttagcag atgttaaagg aaaaggtaaa aaaggcgaaa ttaaggaagt accaacaggg
     6661 tatgcgcaaa actttcttat caaaaagaat ctagccaaag aagcgactgc tcaagctgta
     6721 ggtgaacttc gtggtaaaca aaaatcggaa gaaaaagctc acgctgagat gattgcagaa
     6781 ggaaaagcaa ttaaagcaca acttgaagca gaagaaactg ttgtagaatt tgttgaaaaa
     6841 gttggtccag atggtcgtac ctttggctct attaccaata agaagattgc agaagaattg
     6901 caaaaacaat ttggaattaa gattgataaa cgtcatattc aagtacaagc tccgattcga
     6961 gcggttggtt tgattgatgt gccagtgaaa atctatcaag atatcacaag tgtaatcaat
     7021 cttcgtgtga aagaaggata agtttacacc ttcttgacaa gattgtaaaa ggaagggaag
     7081 tctgatggca gaagtagaag agttacgagt acaacctcaa gatatcttag ctgagcaatc
     7141 cgttttaggg gctatcttta ttgatgagag taaacttgtt tttgtgcgag aatacattga
     7201 gtctcgggac ttttttaagt atgcccatcg tttgattttc caagccatgg tcgatttatc
     7261 cgatcgtggt gatgccatag atgcaacaac ggttcgtact atccttgata atcaaggtga
     7321 tttacagaat attggtggct tgtcttactt ggttgagatt gttaattctg tgccaacttc
     7381 tgctaatgcg gagtattatg ctaagattgt tgcagaaaaa gcaatgctac gtcgtttaat
     7441 tgccaagttg acagagtctg tcaaccaagc ttacgaagcg tcacaaccag ctgatgaaat
     7501 tattgctcag gcagaaaaag ggctgattga tgtcagtgaa aatgcaaatc gaagcgggtt
     7561 taagaacatt cgagatgtgt tgaatctcaa ctttggaaat ctggaagctc gctcgcaaca
     7621 aacgaccgat attacaggta ttgcgacagg ttatcgtgat ttggatcata tgacaacagg
     7681 acttcatgag gaggagttga ttatcttagc agctcgtcca gcagttggta agacagcatt
     7741 tgccttgaat atcgctcaga acattgggac taagttggac aaaacggttg ctattttttc
     7801 actcgaaatg ggtgcggaaa gcttggtaga tcgtatgtta gctgcagaag gcttagtgga
     7861 gtcacattct atccgtacag ggcaattgac agatgaggag tggcaaaaat atactattgc
     7921 tcagggtaat ctagctaacg ccagtatcta tatcgatgat acgccaggta ttcggattac
     7981 agagattcgt tctcgttctc gtaaattggc tcaagaaact ggaaatcttg gtttgatttt
     8041 gatagactat ttgcaactta tcacgggaac tggtcgagaa aatcgtcaac aagaagtttc
     8101 tgaaatttct cgtcagttga aaatactagc caaggaattg aaggttccag taatcgctct
     8161 gagtcagctt tctcgtggtg tagaacaacg tcaggacaag agaccggtct tgtctgatat
     8221 tcgtgaatct gggtctattg agcaggacgc tgatatcgta gcttttctct atcgcgatga
     8281 ctactatgaa cgtggtggtg aagaagagga gggtatccca aataataagg tggaagttat
     8341 tatcgagaaa aaccgtagtg gagctcgtgg aacagtggaa ttgattttcc aaaaagaata
     8401 caataaattt tcaagtatct caaagaggga ggcataagat gtcagatgca tttacagatg
     8461 tagccaagat gaaaaaaatc aaagaagaaa tcaaggcaca tgagggacaa gtcgtagaaa
     8521 tgactttgga gaatggtcgt aagcgccaaa aaaatagatt gggtaagcta attgaagttt
     8581 atccatctct atttattgtg gagtttgggg atgtggaagg agataaacaa gttaatgttt
     8641 acgttgaatc ctttacttac tcagatattc ttacagaaaa gaatttgatt cattatcttg
     8701 actaaagtga gaaattttct cactttttct tttttctccg aataatttag gtgaaggcaa
     8761 tcatcgcttt atattatttt tcaaggagga agaatgaaaa ttttaccgtt tatagcaaga
     8821 ggaacaagtt attacttgaa gatgtcagtt aaaaagcttg ttcctttttt agtagtagga
     8881 ttgatgctag cagctggtga tagtgtctat gcctattcca gaggaaatgg atcgattgcg
     8941 cgtggggatg attatcctgc ttattataaa aatgggagcc aggagattga tcagtggcgc
     9001 atgtattctc gtcagtgtac ttcttttgta gcctttcgtt tgagtaatgt caatggtttt
     9061 gaaattccgg cagcttatgg aaatgcgaat gaatggggac atcgtgctcg tcgggaaggt
     9121 tatcgtgtag ataatacacc gacgattggt tccattactt ggtctactgc aggaacttat
     9181 ggtcatgttg cctgggtgtc aaatgtaatg ggagatcaga ttgagattga ggaatataac
     9241 tatggttata cagaatccta taataaacga gttataaaag caaacacgat gacaggattt
     9301 attcatttta aagatttgga tggtggcagt gttgggaata gtcaatcctc aacttcaaca
     9361 ggcggaactc attattttaa gaccaagtct gctattaaaa ctgaacccct agttagtgca
     9421 actgtgattg attactatta tcctggagag aaggttcatt atgatcaaat tctcgaaaaa
     9481 gacggctaca agtggttgag ttatactgcc tataatggaa gctatcgtta tgttcaattg
     9541 gaggctgtga ataaaaatcc tctaggtaat tctgttcttt cttcaacagg tggaactcat
     9601 tattttaaga ccaagtctgc tatcaaaact gaacccctag ttagcggaac tgtgattgat
     9661 tactattatc ctggggagaa ggttcattat gatcagatac ttgaaaaaga cggctacaag
     9721 tggttgagtt atactgccta taatggaagt cgtcgctata tacagctaga gggagtgact
     9781 tcttcacaaa attatcagaa tcaatcagga aatatctcta gctatggatc caataatagt
     9841 tcaactgtcg gttggaagaa aataaatggt agttggtatc atttcaaatc aaatggttct
     9901 aaatcaacag gatggctgaa agacggttct agctggtatt atttgaaatt atctggtgaa
     9961 atgcagacag gatggttaaa ggagaatggc tcgtggtatt atctgggtag ttcaggggca
    10021 atgaaaacag gctggtacca ggtctctggt aagtggtatt attcttactc ttcaggcgcc
    10081 ttagctgtta atacgacggt ggatggctac agagtaaaca gtgatggaga acgagtatag
    10141 aaaattggag taggagaaat ttcctgctct tttctgtaag cagtttcctt gacatttttc
    10201 ttattttgcg ctatcatata tacatataat atatgggagt ctgtgtacag tctgagagga
    10261 agtgttaaac ttcgaccgca cctgatctgg gtaatgccag cggagggaac gatacttagt
    10321 ctaattttgc accttttcca tgtatggtaa aggtttttct tttttttaaa aggaaaacga
    10381 gaagaggagg ttcttatgaa agcaagcatt gccttgcaag ttttacccct agcacagggg
    10441 attgatcgga tagctgttat tgatcaggtc attgcttatc tgcaaactca agaagtgacg
    10501 atggtagtga caccatttga aacggtcttg gaaggggagt ttgatgagct tatgcgcatt
    10561 ctaaaagaag cgctggaagt ggcagggcag gaggcagaca atgtctttgc caatgtcaaa
    10621 ataaatgtag gagagatttt aagtattgat gagaaacttg aaaagtatac tgagacgaca
    10681 cattagtcta ttgggctttc tcggagtatt gtcaatctgg cagttagcag gttttcttaa
    10741 acttctcccc aagtttatcc tgccgacacc tcttgaaatt ctccagccct ttgttcgtga
    10801 cagagaattt ctctggcacc atagctgggc gaccttgaga gtggctttac tggggctgat
    10861 tttgggagtt ttgattgcct gtcttatggc tgtgctcatg gatagtttga cttggctcaa
    10921 tgacctgatt taccctatga tggtggtcat tcagaccatt ccgaccattg ccatagctcc
    10981 tatcctggtc ttgtggctgg gttatgggat tttgcccaag attgtcttga ttatcttaac
    11041 aacaaccttt cccatcatcg ttagtatttt ggacggtttt aggcattgtg acaaggatat
    11101 gctgactttg tttagtctga tgcgagcaaa cccttggcaa atcctgtggc attttaaaat
    11161 cccggttagc ctgccttact tttatgcagg tctgagggtc agtgtctcct acgcctttat
    11221 cacaactgtg gtatctgagt ggttgggagg ttttgaaggt cttggtgttt atatgattca
    11281 gtctaaaaaa ctgtttcagt atgataccat gtttgccatt attattctgg tgtcgattat
    11341 cagtcttttg ggtatgaagc tggtcgatat cagtgaaaaa tatgtgatta aatggaaacg
    11401 tacttaaaat agagcgtttt tgaaaaagaa aagaggaaat caaaatgaag aaaacatgga
    11461 aagtgttttt aacgcttgta acagctcttg tagctgttgt gcttgtggcc tgtggtcaag
    11521 gaactgcttc taaagacaac aaagaggcag aacttaagaa ggttgacttt atcctagact
    11581 ggacaccaaa taccaaccac acagggcttt atgttgccaa ggaaaaaggt tatttcaaag
    11641 aagctggagt ggatgttgat ttgaaattgc caccagaaga aagttcttct gacttggtta
    11701 tcaacggaaa ggcaccattt gcagtgtatt tccaagacta catggctaag aaattggaaa
    11761 aaggagcagg aatcactgcc gttgcagcta ttgttgaaca caatacatca ggaatcatct
    11821 ctcgtaaatc tgataatgta agcagtccaa aagacttggt tggtaagaaa tatgggacat
    11881 ggaatgaccc aactgaactt gctatgttga aaaccttggt agaatctcaa ggtggagact
    11941 ttgagaaggt tgaaaaagta ccaaataacg actcaaactc aatcacaccg attgccaatg
    12001 gcgtctttga tactgcttgg atttactacg gttgggatgg tatccttgct aaatctcaag
    12061 gtgtagatgc taacttcatg tacttgaaag actatgtcaa ggagtttgac tactattcac
    12121 cagttatcat cgcaaacaac gactatctga aagataacaa agaagaagct cgcaaagtca
    12181 tccaagccat caaaaaaggc taccaatatg ccatggaaca tccagaagaa gctgcagata
    12241 ttctcatcaa gaatgcacct gaactcaagg aaaaacgtga ctttgtcatc gaatctcaaa
    12301 aatacttgtc aaaagaatac gcaagcgaca aggaaaaatg gggtcaattt gacgcagctc
    12361 gctggaatgc tttctataaa tgggataaag aaaatggtat ccttaaagaa gacttgacag
    12421 acaaaggctt caccaacgaa tttgtgaaat aatgacagaa attagactag agcatgtcag
    12481 ttatgcctat ggtcaggaga ggattttaga ggatatcaac ctacaggtga cttcaggcga
    12541 agtggtttcc attctaggcc caagtggtgt tggaaagacc accctcttta atctaatcgc
    12601 tgggatttta gaagttcagt cagggagaat tgtccttgat ggtgaagaaa atcccaaggg
    12661 gcgcgtgagt tatatgttgc aaaaggatct gctcttggag cacaagacgg tgcttggaaa
    12721 tatcattctg cccctcttga ttcaaaaggt ggataaggca gaagctattt cccgagcgga
    12781 taaaattctt gcgaccttcc agctgacagc tgtaagagac aagtatcctc atgaacttag
    12841 cggtgggatg cgccagcgtg tagccttact ccggacctac ctttttgggc acaagctctt
    12901 tctcttagat gaggccttta gcgccttgga tgagatgaca aagatggaac tccacgcttg
    12961 gtatcttgag attcacaaac agttgcagct aacaaccctg atcatcacgc atagtattga
    13021 ggaggccctc aatctcagcg accgtatcta tatcttgaaa aatcgccctg ggcagattgt
    13081 ttcagaaatt aaactagatt ggtctgaaga tgaggacaag gaagtccaaa agattgccta
    13141 caaacgtcaa atcttggcag aattaggctt agataagtag aaaaataggg agttggtgaa
    13201 gattatcctt taccagcgcc ctttttcttt taaaaatgag aaaatttcgg tataatagtc
    13261 aaacaaggtc aaggtttaaa gagagaggtg ggtttgttat gagatttaaa aatacatcgg
    13321 atcatattga ggcctacatc aaggcgattt tagatcaatc tggtatcgtg gagttgcaac
    13381 ggagtcagtt ggcagatacc tttcaggttg ttcctagtca gattaactac gtgatcaaga
    13441 cacgctttac ggaaagtaga ggctacttgg ttgaaagtaa gcgtggtggc ggaggctaca
    13501 ttcgtatagg acggattgag ttttctagtc atcatgaaat gctccgggag ctgctttact
    13561 cgattggtga gcgagtcagt caagaaattt atgaggatat tctccagctt ttggttgagc
    13621 aggaattgat gaccaagcag gagatgaatt tgctagaatc agtagctttg gatcgcgttt
    13681 taggagaaga agctccagtt gttcgagcaa acatgctacg tcaggtcata caagaggtag
    13741 atagaaaagg gaagtaagat gaactattca aaagcattga atgaatgtat cgaaagtgcc
    13801 tacatggttg ctggacattt tggagctcgt tatctagagt cgtggcactt gttgattgcc
    13861 atgtctaatc acagttatag tgtagcaggg gcaactttaa atgattatcc gtatgagatg
    13921 gaccgtttag aagaggtggc tttggaactg actgaaacgg actatagcca ggatgaaacc
    13981 tttatggaat tgccgttctc ccgtcgtttg caggttcttt ttgatgaagc agagtatgta
    14041 gcgtcagtgg tccatgctaa ggtactaggg acagagcacg tcctctatgc gattttgcat
    14101 gatggcaatg ccttggcgac tcgtatcttg gagagggctg gtttttctta tgaagacaag
    14161 aaagatcagg tcaagattgc tgctcttcgt cgaaatttag aagaacgggc aggctggact
    14221 cgtgaagatc tcaaggcttt acgccaacgc catcgtactg tggctgacaa acaaaattct
    14281 atggccaata tgatgggcat gccgcagact cctagtggtg gtctcgagga ctatacgcat
    14341 gatttgacag agcaagcgcg ttctggcaag ttagaaccag tcatcggtcg ggacaaggaa
    14401 atctcacgta tgattcaaat cttgagccgt aagactaaga acaaccctgt cttggttggg
    14461 gatgctggtg tcgggaaaac agctctggtg cttggtcttg ctcagcgtat tgccagtggt
    14521 gatgtgcctg cggaaatggc taagatgcgc gtgttagaac ttgatttgat gaatgtcgtt
    14581 gcagggacac gcttccgtgg tgactttgaa gaacgcatga ataatatcat caaggatatt
    14641 gaagaagatg gccaagtcat cctctttatc gatgaactcc acaccatcat gggttctggt
    14701 agcgggattg attcgactct ggatgcggcc aatatcttga aaccagcctt ggcgcgtgga
    14761 gctttgagaa cggttggtgc caccactcag gaagaatatc aaaaacatat cgaaaaagat
    14821 gcggctcttt ctcgtcgttt cgctaaagtg acgattgaag aaccaagtgt ggcagatagt
    14881 atgactattt tacaaggttt gaaggcgact tatgagaaac atcaccgtgt acaaatcaca
    14941 gatgaagcgg ttgaaacagc ggttaagatg gctcatcgtt atttaaccag tcgtcacttg
    15001 ccagactctg ctatcgacct cttggacgaa gcagcagcaa cagtgcaaaa taaggtaaag
    15061 tatgtaaaag cagacgattc agatttgagt ccagctgata aggccctgat ggatggcaag
    15121 tggaaacagg cagcccagct aatcgcaaaa gaagaggaag tacctgtcta caaagacttg
    15181 gtgacagagt ctgatatttt gaccaccttg agtcgcttgt caggaatccc agttcaaaaa
    15241 ctgactcaaa cggatgctaa gaagtatcta aatcttgaag cagaactcca taaacgtgtc
    15301 atcggtcaag atcaagctgt ttcaagcatt agtcgtgcga ttcgccgtaa tcagtcagga
    15361 attcgcagtc acaagcgtcc gatcggttcc tttatgttcc taggacccac aggtgtcggt
    15421 aagaccgagt tggccaaggc tttggcagaa gttctctttg atgacgaatc agcccttatc
    15481 cgctttgata tgagtgagta tatggagaaa ttcgcagcca gccgtctaaa tggagctcct
    15541 ccaggctatg tgggttacga agaaggtgga gagttgacag agaaggttcg caataaaccc
    15601 tattccgttc tcctctttga tgaggtagag aaggcccacc cagatatttt taatgttctc
    15661 ttgcaggttc tggatgacgg tgttttgaca gatagcaagg gacgcaaggt cgatttttca
    15721 aataccatta tcattatgac atcgaaccta ggtgcgactg cccttcgtga tgataagact
    15781 gttggttttg gggctaagga tattcgtttt gaccaggaaa atatggaaaa acgcatgttt
    15841 gaagaactga aaaaagctta tagaccggaa ttcatcaacc gtattgatga gaaggtggtc
    15901 ttccatagcc tatctagtga ccatatgcag gaagtggtga agattatggt caagccttta
    15961 gtggcaagtt tggctgaaaa aggcattgac ttgaaattac aagcttcagc tctgaaattg
    16021 ttagcaaatc aaggatatga cccagagatg ggagctcgcc cacttcgcag aaccctgcaa
    16081 acagaagtgg aggacaagtt ggcagaactt cttcttaagg gagatttagt ggcaggcagc
    16141 acacttaaga ttggtgtcaa agcaggccag ttaaaatttg atattgcata aaagaataaa
    16201 agtatcagca tctgaccata agtcacagtg gagtgaaact caatgaaaat caaagagcaa
    16261 actaggcagc tagccgcagg ttgctcaaaa cactggtttg aggttgcaga tagagctgac
    16321 gtggtttgaa gagattttcg aagagtatga aactaaaacc tatagcttct aaacgatccg
    16381 tggttttcac cattcaacac aaaattcata tgtttattac cctccgtcgt atttgtctta
    16441 gagcgtgtgt agtagaaaaa gagcagtctt atctgaaatt tttattcttt caaaagagac
    16501 ctgtttcttt tttgcatgtc aaatccgttc tagctggtat ttgaaaaatc aaactaatat
    16561 tcaatgaaaa tcaaagaaca aactaggcag ctagccacag gttgctcaaa acactgtttt
    16621 gaggttgtag atagagctga cgtagtttga agagattttc gaagagtata agctgcaaga
    16681 tgaatgattt tcttgtattg acgttgttgt tgacaaaaag tagcggataa atgaaatcca
    16741 ttccattatc atagatgata ggctggtagg aaattttcaa atagcataca ggaaatagat
    16801 gtatggagtt ctggtagtag aaagggagag agatgaacat tttagttgca gatgacgagg
    16861 aaatgattag agaaggaatt gcagcatttc tgacagaaga gggttatcat gtcattatgg
    16921 ctaaggatgg acaagaggtc ttggaaaaat ttcaagatct ccctatccat ctcatggtat
    16981 tggatttaat gatgcctagg aagagtggtt ttgaagtgtt aaaagaaatc aatcaaaagc
    17041 acgatattcc tgtcatcgtc ttgagtgctc tgggagatga aactactcag tcacaggtat
    17101 ttgatctcta tgctgatgat catgtgacaa aacctttttc tttggtactg cttgtcaagc
    17161 gtattaaggc gcttatcaga cgttactacg tcatagagga tatttggcga tatcaggatg
    17221 taacagtgga ttttacctct tacaaagcac attataaaaa tgaagaaatt gatctcaaac
    17281 caaaggaatt actggtacta aagtgtttga ttcagcataa aaatcaagtt ttaagtagag
    17341 agcagatatt ggaagaaatt tcaaaagatg tagctgattt accttgtgat agggtcgttg
    17401 atgtctatat tcgtactctt cgcaaaaaat tagctttaga ttgtatcgtg actgtgaaaa
    17461 atgttgggta taagattagc ttatgataaa aaatcctaaa ttattaacca agtctttttt
    17521 aagaagtttt gcaattctag gtggtgttgg tctagtcatt catatagcta tttatttgac
    17581 ctttcctttt tattatattc aactggaggg ggaaaagttt aatgagagcg caagagtgtt
    17641 tacggagtat ttaaagacta agacatctga tgaaattcca agcttactcc agtcttattc
    17701 aaagtccttg accatatctg ctcaccttaa aagagatatt gtagataagc gactccctct
    17761 tgtgcatgac ttggatatta aagatggaaa gctatcaaat tatatcgtga tgttagatat
    17821 gtctgttagt acagcagatg gtaaacaggt aacagtgcaa tttgttcacg gggtggatgt
    17881 ctacaaagaa gcaaagaata ttttgttttt gtatcttcca tatacatttt tggttacaat
    17941 tgctttttcc tttgtttttt cttattttta tactaaacgc ttgctcaatc ctctttttta
    18001 catttcagaa gtgactagta aaatgcaaga tttggatgac aatattcgtt ttgatgaaag
    18061 taggaaagat gaagttggtg aagttggaga agttggaaaa cagattaatg gtatgtatga
    18121 gcacttgttg aaggttattc atgagttgga aagtcgtaat gagcaaattg taaaattgca
    18181 aaatcaaaag gtttcctttg tccgtggagc atcacatgag ttgaaaaccc ctttagccag
    18241 tcttagaatt atcctagaga atatgcagca taatattgga gattacaaag atcatccaaa
    18301 atatattgca aagagtataa ataagattga ccagatgagc cacttattgg aagaagtact
    18361 ggagtcttct aaattccaag agtggacaga gtgtcgtgag accttgactg ttaagccagt
    18421 tttagtagat attttatcac gttatcaaga attagctcat tcaataggtg ttacaattga
    18481 aaatcaattg acagatgcta ccagggtcgt catgagtctt agggcattgg ataaggtttt
    18541 gacaaacctg attagtaatg caattaaata ttcagataaa aatgggcgcg taatcatatc
    18601 cgagcaagat ggctatctct ctatcaaaaa tacatgtgcg cctctaagtg accaagaact
    18661 agaacattta tttgatatat tctatcattc tcaaatcgtg acagataagg atgaaagttc
    18721 cggtttgggt ctttacattg tgaataatat tttagaaagc tatcaaatgg actatagttt
    18781 tctcccttat gaacacggta tggaatttaa gattagcttg tagacagatt agttttttat
    18841 taaagttcat atagggttaa cataagtgtg ttattctttg tgtagataaa agaaaggata
    18901 ctaatatggt attagcgatt attttagtaa cattctttat tcgattgatt tttttaaagc
    18961 gttcgataga gaatgagaaa cgaatcctta gcaatggcgg gaaagaattt ggagttgaga
    19021 atacaaaacg attaactatg gctcatattg ttttttatct ctcttgcttg gttgaggcaa
    19081 tggtgcacaa gacaattttt gatggcatgg gcatggttgg tttagtcttg cttatttttt
    19141 ctatgctgat gttaatgttg gtgattcact tgttgggaga tatttggaca gtgaagctta
    19201 tgcttgtcaa taatcacaaa tatgtagatc atatcttgtt taggacagta aaacatccta
    19261 attacttttt aaatattctt cctgagttga ttggcttgac cttgttgagt catgcttatg
    19321 tgacttttgt tttagttttt ccagtttatg cagttatttt gtatcgacga atagctgaag
    19381 aggaaaagct attacatgaa gttataatcc caaatggaag cataaagaga taaatacaaa
    19441 attcgattta tatacagttc atattgaagt gatatagtaa ggttaaagaa aaaatataga
    19501 aggaaataaa catgtttgca tcaaaaagcg aaagaaaagt acattattca attcgtaaat
    19561 ttagtattgg agtagctagt gtagtagttg ctagcttgtt cttaggagga gtagttcatg
    19621 cagaaggggt tagaagtggg gataccccca aggttacatc tagtggggat gaagtcgagt
    19681 cgcatctaaa aaaaatattg agtgagatcc aaacacaatt agatagaaaa cgacatgcca
    19741 aaactgtagc gctaattaac aagttgcaag acattaagag aacgtattcg tataaattaa
    19801 atgttttaga agagaagccg aaagctgagt tgacgtcaaa aacaaaagaa gagttaaccg
    19861 cagcttttga gcaatttaaa aaagatacat taccaacaga accaggaaaa aaggtagcag
    19921 aagctgagaa gaaggttgca gaagctgaga aaaaagccga ggatcaaaaa gaagaagatc
    19981 gtcgtaacta cccaaccaat acttacaaaa cgcttgaact tgaaattgct gagttcgatg
    20041 tgaaagttaa agaagcggag cttgaactat taaaagagaa agctaagaaa cctttaaacg
    20101 agggcacaat taagcaagca aaagagaaag ttgagagtaa aaaagctgag gctacaaggt
    20161 tagaaaacat caagacagat cgtaaaaaag cagaagaaga agctaaacga aaagcagcag
    20221 aagaagataa agttaaagaa aaaccagctg aacaaccaca accagcgccg gctcctcaac
    20281 cagaaaaacc agctccaaaa ccagaaaaac cagctccagc tccaaaacca gaaaaaccag
    20341 ctccagctcc aaaaccagag aatccagctg aacaaccaaa agcagaaaaa ccagctgatc
    20401 aacaagctga agaagactat gctcgtagat cagaagaaga atataatcgc ttgactcaac
    20461 agcaaccgcc aaaaactgaa aaaccagcac aaccatctac tccaaaaaca ggctggaaac
    20521 aagaaaacgg tatgtggtac ttctacaata ctgatggttc aatggcgaca ggatggctcc
    20581 aaaacaatgg ctcatggtac tacctcaaca gcaatggcgc tatggcgaca ggatggctcc
    20641 aaaacaatgg ttcatggtac tatctaaacg ctaatggttc aatggcaaca ggatggctcc
    20701 aaaacaatgg ctcatggtac tacctaaacg ctaatggttc aatggcgaca ggatggctcc
    20761 aatacaatgg ttcatggtac tacctaaacg ctaatggtga tatggcgaca ggatggctcc
    20821 aatacaatgg ttcatggtac tacctaaacg ctaatggtga tatggcgaca gattgggtga
    20881 aagatggaga tacctggtac tatcttgaag catcaggtgc tatgaaagca agccaatggt
    20941 tcaaagtatc agataaatgg tactatgtca atggctcagg tgcccttgca gtcaacacaa
    21001 ctgtagatag ctatagagtc aatgccaatg gtgaatgggt aaactaaacc taatataact
    21061 agttaatact gacttcctgt aagaactctt taaagtattc cctacaaata ccatatcctt
    21121 tcagtagata atataccctt gtaggaagtt tagataaaaa aataactctg taatctctag
    21181 ccggatttat agcgctagag actacggagt ttttttgatg aggaaagaat ggcggcattc
    21241 aagaggctct ttaagagagt tacgggtttt aaactattaa gccttctcca attgcaagag
    21301 ggcttcaatc tctgccaggg tgctagcttg cgaaatggct ccacggagtt tggcagcgcc
    21361 agatgttcca cggagatagt gaggagcgag gccgcggaat tcacgaactg cgacgttttc
    21421 tcctttgagg ttaatcaatc gtttcaagtg ttcgtaggcg atcttcatct tgtcttcaaa
    21481 ggtcaaatca ggtaggattt ctcctgtttc aaagtaatgg ttgatttggt tgaagaggta
    21541 aggatttccc atggcagctc ggccaatcat gactgcgtca gcaccaactt cttcgatgcg
    21601 ttgcttggct tcttggacag tacggatatc accgttggcg atgaatggaa tcttggttag
    21661 agcttgggca accttgtaaa gggtctcaag gtctgcgtgg ccagtataca tttgttcacg
    21721 ggtacggcca tgcatggcga gggcagaaac acctgcagcc tcagcagcga gggcattttc
    21781 tactgcaaga gatgggtccg cccatccggt acgcattttg acagtaagtg ggatatcaag
    21841 gacagactgg accttgttga tgatagagta gatcttgtca ggatccttga gccacatagc
    21901 tccagcttcg ttcttcacga ttttgttgac agggcagccc atgttgatat cgacgatatc
    21961 ggtcttggtg ttttcttgga tgaattctgc tgcgcgtgct aggctgtctt catcgctacc
    22021 aaaaagttgg atagagacag ggttttcgcc ctcatcgata tgaagcatgt gcagggtttt
    22081 ttcgttgttg tattggattc ccttgtcaga gaccatttcc attacaacga gtccagctcc
    22141 gagctctttt gcgatggtac gaaaggctga gttggtcacg ccagccatag gcgctaaaac
    22201 ggtacgattg ggaatctcaa tattgccaat cataaaaggt gtattaagat ttgtcacgaa
    22261 tgagttcctc caggtccttt tcatcaaagt tgtaagtagt ttggcagaat tgacaagtga
    22321 tttctgcccc gtggtcttcc tctttcattt cctgtaagtc tgagcttgga aggctggcaa
    22381 gagcgttcat aaagcgttca tggctacagt cacattggaa acggatttct tcttcagaaa
    22441 gacgcttgta ggcttcgtcc ccgtagatag ccttgaggag ggcttcgata tggtcgtcgc
    22501 tttcgagaag agtagagata gctggcattt cttggatgcg tttttcaaag cgagcaatct
    22561 cttctttctt ggctcctggc aagacttgaa ctaggaaacc acctgcaacc ttgaccttgt
    22621 cttcctcgtc caaaaggaca ttgaggccga ccgctgaagg cgtttgttgg ctttcagtaa
    22681 ggtaaaaggc aaggtcttca ccgatttctc cagagatgag gggagttata gagttgtaag
    22741 gatttccagc accgtagtct gtgataacga ggaattgacc atttccaaca aaaggtccga
    22801 ctaggacttc accagtcgca gtctttttga tgtcaacacc aggattttga acatagcctt
    22861 tgacgttccc cttggtatca gcgacggtga taatagcacc tagagagcta gagcccaaca
    22921 ccttaactgt aagtttggta tttccttttt cattggctgc gagaatctgg ctagcgataa
    22981 gagttcgacc aagcgctaca gttgagctag cttgggtttg atgtctttct tgagcagtgc
    23041 ggacggtttc cgtgctatca aggacaaaag cacgaaaggc tccgctttct gatatagttt
    23101 taataatttt atccatagct actattttag cataaaaatg cccaaagggg gagccgtgtg
    23161 tttactgatt ttcaggataa tggaccagga aatcagcatg aaaataaaaa gagaaacaga
    23221 ttatttcagc atttgtaaga tttatgctat gcttaaggta gaaaatgaaa ggggtaacaa
    23281 atgtatttag gagatttgat ggagaaagcc gagtgtggtc aattttcaat cctttccttt
    23341 ctattacaag agtctcagac gaccgtcaag gctgtaatgg aagaaacagg attttcaaaa
    23401 gcaaccctaa ccaaatatgt caccctgctc aatgacaagg ctttggatag tggcttagaa
    23461 ctgactattc actcagaaga tgaaaatctg cgtctgtcta tcggtgcagc taccaagggg
    23521 agagatattc ggagcttgtt tttggatagt gctgttaaat accagatttt ggtttatctt
    23581 ctctaccacc aacagttttt agcccatcag ctggctcaag aattggtgat tagcgaggct
    23641 acgcttggtc gtcacttggc tggtttaaat cagattttgt cagaatttga tttatccatc
    23701 caaaatggac gctggagagg tccagagcat cagattcgct atttctattt ctgtcttttc
    23761 cgaaaggtct ggtcgagtca ggaatgggaa ggtcacatgc agaaaccaga gagaaaacag
    23821 gagattgcca atttagagga aatctgcggt gcaagtttgt ctgcggggca gaaattggac
    23881 ttggttctct gggctcacat cagtcaacaa cgtcttcggg tcaatgcttg tcagtttcaa
    23941 gtcatagaag agaaaatgcg agggtatttt gacaatatct tttatcttcg tttgctgaga
    24001 aaggttccgt ccttttttgc tgggcaacat attccactag gagttgagga tggtgagatg
    24061 atgatattct tctcttttct cctatctcat cgcattcttc ctcttcatac tatggagtat
    24121 attcttggtt ttggagggca gttggcagat ttactgacgc aattgattca agaaatgaag
    24181 aaggaggaac tattggggga ttatacagag gaccatgtca cctatgaact cagtcagctt
    24241 tgtgctcaag tctatctcta taagggctat attttacagg atcgctacaa gtaccagtta
    24301 gagaatcgtc atccatattt actgatggaa catgatttta aagagacagc agaggagatt
    24361 tttcatgctc tacctgcttt tcaacagggg acagatttag ataagaagat tctctgggaa
    24421 tggctccagt taatcgaata tatggctgaa aacggtggcc agcatatgcg gattggtctg
    24481 gatttgacat ctggttttct tgtcttttca aggatggcag ccattttgaa acggtatttg
    24541 gaatacaatc gttttattac cattgaagct tatgacccta gtcggcatta tgatttgctg
    24601 gttaccaata acccgattca taagaaggaa cagacaccag tctattattt aaaaaacgac
    24661 ttggatatgg aagatttggc agggattcgt cagttattat tcacttaaaa ggcttggtta
    24721 atctaggtct tttttgtgaa attcacacaa tctcctcaca tttttttaaa aattaaaaaa
    24781 agttgataaa caagaaagcg ctttattttg tatactagta agtgtaaaga ggaaacacct
    24841 caagatcttt atcaggagga cagtacatgt cacaagaaaa atacatcatg gccattgacc
    24901 agggaactac aagttctcgt gccatcattt tcaacaaaaa aggggaaaag gttagctcga
    24961 gtcaaaaaga gtttacccag attttccctc aggcaggttg ggttgagcac aatgccaatg
    25021 aaatttggaa ctctgttcag tcagttattg cgggtgcttt catcgaaagt ggtgtcaagc
    25081 caaatcaaat cgaggcaatc gggattacca accaacgtga aacaacggtt gtctgggata
    25141 agaaaacagg acttcctatc tacaatgcta tcgtttggca gtcacgccag acagcacctt
    25201 tggctgagca actaaaaagc caaggttatg tggaaaaatt ccatgaaaag actggtttga
    25261 ttattgatgc ttacttctct gctaccaagg ttcgttggat tttggatcat gtagaaggtg
    25321 ctcaagagcg agcagaaaaa ggggaattgc tctttggtac tatcgatact tggttggttt
    25381 ggaaattgac tgacggtgcg gctcacgtga ctgactactc aaatgcagct cgtaccatgc
    25441 tttataacat taaagaactc aaatgggatg atgagatttt ggaaatcctt aacattccga
    25501 aggctatact tccagaagtt cgttctaact ccgaaatcta cggcaagaca gctccattcc
    25561 atttctacgg tggagaggtg ccaatctcag gtatggctgg ggaccaacaa gcagccctct
    25621 ttggacagtt ggcttttgag ccaggtatgg ttaagaatac ttatggaaca ggctctttca
    25681 tcatcatgaa tactggggaa gagatgcagt tgtctgaaaa caacctcttg acaaccattg
    25741 gttacggaat caacggtaag gtttattatg ccttggaagg ttctatcttc atcgcaggaa
    25801 gtgctattca gtggcttcgt gacggtcttc gcatggttga aaattcacca gaatctgaaa
    25861 aatacgctcg tgattctcac aacaacgatg aagtttatgt cgttccagcc tttacaggtc
    25921 taggcgctcc atactggaac caaaatgctc gtggttccgt ctttggtttg actcgtggaa
    25981 caagcaaaga agactttatc aaggcgactt tgcaatctat tgcttatcaa gtgcgtgata
    26041 tcatcgacac catgcaagtg gatactcaga ccgccattca agtactgaag gtggatggtg
    26101 gtgcagccat gaacaacttc ctcatgcagt tccaggcgga tattttaggc attgacattg
    26161 cacgtgctaa aaacctggaa acaacagctc taggagcggc cttcctagca ggtttgtcag
    26221 tagggtactg gaaagacttg gacgagttga aactcttgaa cgagacagga gaactctttg
    26281 agccatctat gaacgaatct cgcaaggaac aactctacaa gggctggaag aaggctgtga
    26341 aagcaactca agtctttgcg gaagtagacg actaatactg gcagaataaa gcgatttatt
    26401 tagaaagtgt gtaaatatgg aattttcaaa gaaaacacgt gaattgtcaa ttaaaaaaat
    26461 gcaggaacgc accctggacc tcttgattat cggtggagga atcacaggag ctggtgtagc
    26521 cttgcaggcg gcagctagcg gtcttgagac tggtttgatt gaaatgcaag actttgcaga
    26581 aggaacatct agtcgttcaa caaaattggt tcacggagga cttcgttacc ttaaacaatt
    26641 cgacgtagaa gtggtctcag atacggtttc tgaacgtgca gtggttcaac aaatcgctcc
    26701 acacattcca aaaccagacc caatgctctt accagtttac gatgaagatg gagcaacctt
    26761 tagcctcttc cgtcttaaag tagccatgga cttgtacgac ctcttggcag gtgttagcaa
    26821 cacaccaact gcgaacaagg ttttgagcaa ggatcaagtc ttggaacgcc agccaaactt
    26881 gaagaaggaa ggcttggtag gaggtggagt gtatcttgac ttccgtaaca acgatgcgcg
    26941 tctcgtgatt gaaaacatca aacgtgccaa ccaagacggt gccctcattg ccaaccacgt
    27001 gaaggcagaa ggcttcctct ttgacgaaag tggcaagatt acaggtgttg tagctcgtga
    27061 tctcttgaca gaccaagtat ttgaaatcaa ggcccgtctg gttattaata caacaggtcc
    27121 ttggagtgat aaagtacgta atttgtctaa taagggaacg caattctcac aaatgcgccc
    27181 aactaaggga gttcacttgg tagtggattc aagcaaaatc aaggtttcac agccagttta
    27241 ctttgacaca ggtttgggtg acggtcgtat ggtctttgtt ctcccacgtg aaaacaagac
    27301 ttactttggt acaactgata cagactacac aggtgatttg gagcatccaa aagtaactca
    27361 agaagatgta gattatctac ttggcattgt caacaaccgc tttccagaat ccaacatcac
    27421 cattgatgat atcgaaagca gctgggcagg tcttcgtcca ttgattgcag ggaacagtgc
    27481 ctctgactat aatggtggaa ataacggtac catcagtgat gaaagctttg acaacttgat
    27541 tgcgactgtt gaatcttatc tctccaaaga aaaaacacgt gaagatgttg agtctgctgt
    27601 cagcaagctt gaaagtagca catctgagaa acatttggat ccatctgcag tttctcgtgg
    27661 gtctagcttg gaccgtgatg acaatggtct tttgactctt gctggcggta aaatcacaga
    27721 ctaccgtaag atggctgaag gagctatgga gcgcgtggtt gacatcctca aagcagaatt
    27781 tgaccgtagc tttaaattga tcaattctaa aacttaccct gtttcaggtg gagaattgaa
    27841 cccagcaaat gtggattcag aaatcgaagc ctttgcgcaa cttggagtat cacgtggttt
    27901 ggatagcaag gaagctcact atctggcaaa tctttacggt tcaaatgcac cgaaagtctt
    27961 tgcacttgct cacagcttgg aacaagcgcc aggactcagc ttggcagata ctttgtccct
    28021 tcactatgca atgcgcaacg agttggctct tagcccagtt gacttccttc ttcgtcgtac
    28081 caaccatatg ctctttatgc gtgatagctt ggatagcatc gttgagccaa ttttggatga
    28141 aatgggacga ttctatgact ggacagaaga agaaaaagca acttaccgtg ctgatgtcga
    28201 agcagctctc gctaacaacg atttagcaga attaaaaaat taagaaaaaa taaaagaggt
    28261 ggagggcagc attccttgtc gcccgtccct tctttttaat ggagacagaa agatgatgaa
    28321 tgaattattt ggagaatttc tagggacttt aatcctgatt cttctaggaa atggtgttgt
    28381 tgcaggtgtg gttcttccta aaaccaagag caatagctca ggttggattg tgattactat
    28441 gggttggggg attgcagttg cggttgcagt ctttgtatct ggcaagctca gtccagctca
    28501 tttaaaccca gctgtgacca tcggtgtggc cttaaaaggt ggtttgcctt gggcttccgt
    28561 tttgccttat atcttagccc agttcgcagg ggccatgctg ggtcagattt tggtttggtt
    28621 gcaattcaaa cctcactatg aggcagaaga aaatgcaggc aatatcctgg caaccttcag
    28681 tactggacca gccatcaagg atactgtatc aaacttgatt agcgaaatcc ttggaacctt
    28741 tgttttggtg ttgacaatct ttgctttggg tctttacgat tttcaggcag gtatcggaac
    28801 ctttgcagtg ggaactttga ttgtcggtat cggtctatca ctaggtggga caacaggtta
    28861 tgccttgaac ccagctcgtg accttggacc tcgtatcatg cacagcatct tgccaattcc
    28921 aaacaaggga gacggagact ggtcttacgc ttggattcct gttgtaggcc ctgttatcgg
    28981 agcagccttg gcagtgcttg tattctcact tttctagttt atactcttcg aaaatcaaat
    29041 tcaaaccacg tcagcgtcgc cttaccgtac tcaagtacag cttacggcta gcttcctagt
    29101 ttgctctttg attttcattg agtattagaa aacaattatg ttgatagagc ttgggcaaga
    29161 gcccaatttc agcaaaaaat gaagtaaatc ttctcataat aaaacgcatc atatcaagca
    29221 cgaaaattcc acgaggtcaa ctacagtcag aaagctgaac aacaagccaa aacgcccaaa
    29281 aaaggcggca aaaagcaagc acctgcaagc aacgtgccga aatggtcaaa tcctgattat
    29341 gtcaacgaat tagacccaaa aatcgttgat atgctagtag aatttcacaa gtcacaaggc
    29401 actttggaaa ctcccgaggc gcaagcagaa atcgcccaaa aacgtgaaga aatcgagcaa
    29461 aggagagctg agcttgagga taaaaaacaa gagcttttga accgcttgaa caaatagagt
    29521 ttcgcaagta ttatgcttac aaattacttg agcaattaac taaaatataa accctgcctt
    29581 tatatctagg cagggtttat attttagaaa ttcacgtagg ttgttacggt ttttacatac
    29641 ccagtatagt ttgagtttct atagtattca gtgataaact tccatttttt ttgagcaaca
    29701 tggatataag tacttgttat gtagtatgga tatgggcttt gtgaatccaa gtaagactga
    29761 taagcttgta taccaaaata tgctccacca attattgcac cccatggacc ccccaataaa
    29821 gtacctatcc taccaatcat ataactgatt ccagcaccag tcatgaagtt agcgaatgtg
    29881 ttagcttgtt tattcccatg tattgtgttg acgtaattcc aaacattagg atcgtatgat
    29941 ctaaaagata tatttaggtc gatttcattc ttttgataag ccatataaaa tgccccattg
    30001 atatagacgc cgtcagcacg tcgttcaata gtgtctacac ttccatctgg attgacaacc
    30061 tcaagaactt catcgcttaa aatatttact tgcgtatctc cgaaccgcac tgatgagcca
    30121 ttctcaaact gagcctcacc agatacaact ttagagtttg ccgataagct atcatcagca
    30181 aaaacaaaca agcgacgggg aaatgctaga catacagaaa acagacataa ctagcaaaca
    30241 catgcattta aacatcttag acataacgga aactcctttg tatttttgat ttttttcaac
    30301 ttttattata caataaaacc aaataaaaag aaagcggtaa caatatgctt aatgcgaaaa
    30361 ttttttatat atttttatgt ttgatcgtta tcgaaactac aggcttgttg ttgttgaaaa
    30421 gaggtctcga aatgggttat ttagacacag aagctattat cctcgcagtt ttttcatttg
    30481 ctttttacaa cctatgttca ttcgcttggg tctgctctac aataaaaaac aataaaaaat
    30541 aaatagacgt at
//
LOCUS       DAVBCN010000019        30418 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVBCN010000019 DAVBCN010000000
VERSION     DAVBCN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30418
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.019"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(322..1170)
                     /gene="dprA"
                     /locus_tag="VQ497_000781"
     CDS             complement(322..1170)
                     /gene="dprA"
                     /locus_tag="VQ497_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HET2517121.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1268..1957)
                     /locus_tag="VQ497_000782"
     CDS             complement(1268..1957)
                     /locus_tag="VQ497_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET2517122.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQNIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     HKLEEILKNEN"
     gene            complement(1969..2847)
                     /locus_tag="VQ497_000783"
     CDS             complement(1969..2847)
                     /locus_tag="VQ497_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET2517123.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2837..3706)
                     /gene="licA"
                     /locus_tag="VQ497_000784"
     CDS             complement(2837..3706)
                     /gene="licA"
                     /locus_tag="VQ497_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HET2517124.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            complement(3723..4745)
                     /locus_tag="VQ497_000785"
     CDS             complement(3723..4745)
                     /locus_tag="VQ497_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET2517125.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFKNAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4750..5457)
                     /locus_tag="VQ497_000786"
     CDS             complement(4750..5457)
                     /locus_tag="VQ497_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET2517126.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDSIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5793..7280
                     /gene="tacF"
                     /locus_tag="VQ497_000787"
     CDS             5793..7280
                     /gene="tacF"
                     /locus_tag="VQ497_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HET2517127.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGDILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7290..8093
                     /locus_tag="VQ497_000788"
     CDS             7290..8093
                     /locus_tag="VQ497_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2517128.1"
                     /translation="MKQLTVEDAKQIELEILNYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPMDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRLVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSATFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8095..8904
                     /locus_tag="VQ497_000789"
     CDS             8095..8904
                     /locus_tag="VQ497_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2517129.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDQFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNIPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(9179..12355)
                     /gene="carB"
                     /locus_tag="VQ497_000790"
     CDS             complement(9179..12355)
                     /gene="carB"
                     /locus_tag="VQ497_000790"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET2517130.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(12667..13746)
                     /gene="carA"
                     /locus_tag="VQ497_000791"
     CDS             complement(12667..13746)
                     /gene="carA"
                     /locus_tag="VQ497_000791"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET2517131.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            complement(13796..14719)
                     /locus_tag="VQ497_000792"
     CDS             complement(13796..14719)
                     /locus_tag="VQ497_000792"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET2517132.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            complement(14738..15259)
                     /gene="pyrR"
                     /locus_tag="VQ497_000793"
     CDS             complement(14738..15259)
                     /gene="pyrR"
                     /locus_tag="VQ497_000793"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET2517133.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            complement(15470..16099)
                     /gene="nth"
                     /locus_tag="VQ497_000794"
     CDS             complement(15470..16099)
                     /gene="nth"
                     /locus_tag="VQ497_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET2517134.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            complement(16099..16641)
                     /locus_tag="VQ497_000795"
     CDS             complement(16099..16641)
                     /locus_tag="VQ497_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET2517135.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFISGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            16820..17137
                     /locus_tag="VQ497_000796"
     CDS             16820..17137
                     /locus_tag="VQ497_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HET2517136.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSPLIGLGSLKLNDQARTAWRNYDKIHYEHVKHVLSLYGPV
                     FGF"
     gene            17376..18935
                     /locus_tag="VQ497_000797"
     CDS             17376..18935
                     /locus_tag="VQ497_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2517137.1"
                     /translation="MHYRHSRKGNSMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKTLMGEDLSDFTIKGDIQSDYQSLAYIPQKVPEDLKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKTAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLAQELQLTVRGQEKIGIIGPNGVGKSTLLAKLQQLLNDKREISLGFMPQDYHK
                     KLQLDLSSIAYISKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(18979..19878)
                     /gene="htpX"
                     /locus_tag="VQ497_000798"
     CDS             complement(18979..19878)
                     /gene="htpX"
                     /locus_tag="VQ497_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HET2517138.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(19880..20440)
                     /locus_tag="VQ497_000799"
     CDS             complement(19880..20440)
                     /locus_tag="VQ497_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET2517139.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTSEEEKSVPKVDFSGLGD"
     gene            20534..21247
                     /gene="rsmG"
                     /locus_tag="VQ497_000800"
     CDS             20534..21247
                     /gene="rsmG"
                     /locus_tag="VQ497_000800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HET2517140.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            21501..22784
                     /locus_tag="VQ497_000801"
     CDS             21501..22784
                     /locus_tag="VQ497_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HET2517141.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(22979..24550)
                     /gene="ffh"
                     /locus_tag="VQ497_000802"
     CDS             complement(22979..24550)
                     /gene="ffh"
                     /locus_tag="VQ497_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET2517142.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(24562..24894)
                     /locus_tag="VQ497_000803"
     CDS             complement(24562..24894)
                     /locus_tag="VQ497_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HET2517143.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            complement(24985..25362)
                     /locus_tag="VQ497_000804"
     CDS             complement(24985..25362)
                     /locus_tag="VQ497_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HET2517144.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELMMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            25434..26738
                     /locus_tag="VQ497_000805"
     CDS             25434..26738
                     /locus_tag="VQ497_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET2517145.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTVTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPVENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKNFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESQNPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            26751..27557
                     /gene="yidA"
                     /locus_tag="VQ497_000806"
     CDS             26751..27557
                     /gene="yidA"
                     /locus_tag="VQ497_000806"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HET2517146.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRPMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            27600..28598
                     /locus_tag="VQ497_000807"
     CDS             27600..28598
                     /locus_tag="VQ497_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HET2517147.1"
                     /translation="MNFFSKLFNLKQNNHNRDTNSDCNNFYLNELECGLTPGQLILID
                     WTQKTGRNYNFPRYFKYSLQIDPESTHNQLYKLGYFTKNKTLSYLTVVELKTILSKHN
                     LATSGKKAELITRIINNVNIDNLDIPFEFKLTKEAQNLIIEHSDYIKAYYDKDISMED
                     YCKEKNNISFKATFGDIKWSLLNKQAHRNTVSGDFGCLSNTRKAQGRHLEQEGNIKHA
                     LIYYIESLIITISGLENNFSATDYPVYYPDSIPDYSLKHIQTLMESLSDDDYDFAFDE
                     ALFRFSILNANHFLSKEDIDYLRVNLPRSTAEEINNYLKKYECYSPLNNLELDDFE"
     gene            complement(28765..28836)
                     /locus_tag="VQ497_000808"
     tRNA            complement(28765..28836)
                     /locus_tag="VQ497_000808"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28801..28803),aa:Arg,seq:cct)
     gene            complement(28879..29226)
                     /gene="rplS"
                     /locus_tag="VQ497_000809"
     CDS             complement(28879..29226)
                     /gene="rplS"
                     /locus_tag="VQ497_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET2517148.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(29345..29674)
                     /gene="crcB"
                     /locus_tag="VQ497_000810"
     CDS             complement(29345..29674)
                     /gene="crcB"
                     /locus_tag="VQ497_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET2517149.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(29668..30042)
                     /gene="crcB"
                     /locus_tag="VQ497_000811"
     CDS             complement(29668..30042)
                     /gene="crcB"
                     /locus_tag="VQ497_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET2517150.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYFSLV
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            complement(30039..30305)
                     /locus_tag="VQ497_000812"
     CDS             complement(30039..30305)
                     /locus_tag="VQ497_000812"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET2517151.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSYILKRSRDYQDQNIK"
BASE COUNT         9241 a         6535 c         5541 g         9101 t
ORIGIN      
        1 tttcccttat actcaatgaa aatcaaagcg caaactagga agctagccac aggttgctca
       61 aagcactgct ttgaggttgt agataagact gacgaagtca gctcaaaata ctgttttgag
      121 gttgcagatg gaagctgacg tagtttgaag agattttcga agagtattat atgagaaaaa
      181 tcctctactc tactaatcca cttagaaaaa acgcatatta tcaagtgtta aaaacttgct
      241 aatatgcgtt ttattatgga aatgttgggt tcattttctc ttaaaattga ttttttctta
      301 gaattctagc ttaggtcatt tttaaaattc aaattccgca agaacatctt gcccactggt
      361 gaccaatttt gctccttctt gaatcaaatg atggcaaccg tctgatagtc catctaaaat
      421 gctaccagga atagcaaaga catcgcgtcc ttcttccatt gctcgctcac acgtaatgag
      481 actacctgaa cgcatcttag cctctgctac aatcacacca cgacaaagtc cagcaatgat
      541 gcgattacgg gcaggaaaat gaaatttcag aggttgttcg ccaggtccat attcacttag
      601 aaccagatgg tcattgccga tgtagtcttg caagcgttta ttggctttag gataaaacac
      661 atccagtcct gttccaatca ctgcaatggt ttttccgcca ttctgaagag ctgccatatg
      721 agctgctgtg tcaatgccct tggccagacc actgacaata accagttcat tttccaagcc
      781 ttgaatgact ttttcaactg atttagctcc ctgtttgcta caagcacgac tgcccacgac
      841 cgctaccttc gggaatttca ggaggtcaag atttcccttg taaaataaaa gtacaggcgc
      901 atcatatatt tcactcaaat cccaaggata acagtcatct aaaatagaga aagatggaaa
      961 tttttgaaac tctttcgaca aatgcgcatc gtctatctga aaataacgtt ccataaaaac
     1021 ggctggatta cggcaacctg atatatctgc aatatcaccc aacaaaagct cctgatcaac
     1081 attttcaccg tattctagaa ctttcaaaat ctgttgattg gtcaaacctg atttttttaa
     1141 cttatagatt tcatagtttg tgattttcat aaataactcc atttcttttt ctactcatct
     1201 atctattcgt aaaagttcgt aaaaaatcaa aaaaacatcc gactattttg tcagatgttg
     1261 gaagctttta attttcgttt ttaagaattt cttctaattt atgatagtct tggacactat
     1321 caatctcata aatgctattg ccttctaatt cttcaacata gacatctagc tctttgatgt
     1381 tatccttaac catattgtcc caatagagat caacaaattc gccgcttgca taagccttat
     1441 cgataaagct aacaattttt tctgctgttg gagcatccca gaaggataca ccactaagga
     1501 tgcgacctgc cttgctatca acaataatgt tttgaacctt gtagtcatct ccatagacca
     1561 agaaccattc gttggtacaa tcttcacgat agacactaaa ataagtagaa cgtgtcaaat
     1621 cattgcggaa catattttta aagagatagt tatcggcatc gataacataa ctattggcta
     1681 attcttcttt tacaagatag agagagtaaa agttattgta gtcagcgtat ttatcattga
     1741 aaacgagacg aacaccgtat ttctctttca agtaatcgaa ttgttcttta agataaccaa
     1801 caatgatgat gatgtcattg attccttttt ctttgagaaa ctcaatttgg tactcaatca
     1861 aaggtttttg attaacctga accaaggctt taggggtatt ttcagtcata ggacgcaagc
     1921 gagttcccaa tcccgctgct aagatgatgg ctttcacacg aatctccttt attctttaat
     1981 aataatataa actccagcaa tgacgacaag tgacgtcata atggtcagca tatctagcgg
     2041 tgcacccaag aaaacaactg caaacaagac cgtccatact acatagctca cgttcaagcc
     2101 tgtagccttg gctggttgca agcgattgat agcgatataa taagccaagt aggaaatcat
     2161 atcaaaggct gcaaaaacaa tcatgagacc tagcaattgt ccattggcta ctgcagtaaa
     2221 tgactgatga gagaagagca caatcacaag ataggacaag aacgaagtta cttgacggat
     2281 taagagggct tcgatttcac tcaattcact ttccatggca aaagagctaa gaacactctc
     2341 acttccccat gcaatagcac aaaccaaagc acaaagaatc ccaatgtaga aagaattaac
     2401 ctgttcaact ttataggtct gggcaataat ccctccaata atcaagacaa tcccaaatac
     2461 agtatttttc gaaatcttgt gcttcaaaaa gaagaaagcc aatagaactg aaatcgcagg
     2521 gtaaatagcc gatacagatg aagctaaaga acttccgata tacttaactg cataaagatt
     2581 ggcctgcata ccgatagggc ctgctagcaa ggctccgatg ataacactga cattgcgaat
     2641 atttaagaaa attgagaggc gaactttccc ttcttttacc aagagaaaag ctagtaagat
     2701 aaagatgctc aaaaaatcat gagctgcagc caccacaaag ggtgacaaat ctgtaaaaat
     2761 cgaaaagata taagcactaa ccgttagacc caagccccag aaaatacctg agagaaggcc
     2821 aaaaggaact ccgtttttac ttttcatctg aacctccata agaagccaaa cctttaacag
     2881 ctctttggta acgattcaca ccatagtcac caaaatcttc accttgctct tccttataga
     2941 cagtccatag actccaaata gtatcttgta aaattttata aatagcaatc ttttcatgag
     3001 aaaccggtgt ttggtcactc tcatagtgag ataagaaagt ttcttcctct tgggaagtga
     3061 attcagactc taaaaagagg gcagccaaat cccacattgg atcattcatt gatgaatatt
     3121 cccagtcaat caaataaagt cgtccttgag gtgattcgat aaagttttca ggcaccaaat
     3181 cgatatgaca agattttctg tcaacaccta agtcagccag tcttttctct aaggagaaga
     3241 ctgcatttct aacagattca tagttggcat aaggaatttg ttcttcaatc aaggattcgt
     3301 attttttgat ttcttcaaaa ggagcaaatt ctcctcttaa ttccttagca gacgtatgaa
     3361 tagtttgtaa tattggagca attttgtcga acttggtctt gattgacgtt gaatcaagcg
     3421 taatcgcaga ttcgatatac tcatttactt tgataccagc ttcaatatca aaaagataat
     3481 tttttacatc taagcctaaa tcctttagta gttcaagatt gtacttttca tcttgtcgat
     3541 tgataagctt ttctgtccct ttaccaaaga atttaacaat gtattgctta tttgttgttt
     3601 tggccaaata gttttgattg gtcattccac ccagttgttc aacactgagg acttcctctt
     3661 cttgactaag taaggaagaa attttttctt taatgatttt ctccacaatt aacctccagt
     3721 acttatactt cccacttaaa cactgtttta aaggctgtgt ttaaatcggt tgcaaagaca
     3781 cgatgaatat ctttaatttc tcttacaggt tcttctagat aaaggatatt tttaagacga
     3841 ttggcaaatt tcttgacttc catcatttgg atagcatttt taaaatcaat gcgaccagaa
     3901 cgagatgacc caaccaaaat caagcccttt tctaaggcat cgcgagtatt gagattgact
     3961 ttatattcgc taactcccat catgagaatc gttccctgag gacgaatgta gcgaatcaag
     4021 tcattaatag ctggtccagt accatcacca ccacaacatt caaaagcatg gtcaaaggcc
     4081 aactcttcag gaatattatc cgtaatatag cattctttgg caaatgagaa gagttccaac
     4141 ttttcccaat gacgaccaat aaccacaatc tctgcttctg gcaaagtata gttgataata
     4201 ttggcaacca caaaagctaa acttccatct ccaataacgg cgatccgctc ccgcttgcta
     4261 tgagcaagag tcaatagacg attcatagcg tgcatgccca cactgacaaa ctctgtaatg
     4321 gctgcaaccg tatcttcaat agcatcataa gccaccacac gatctttagg gagagaaaca
     4381 aactctctca taaagccatc aaatccacta gacaagaaat gggtccctgt catgtagttt
     4441 tcatagaatt cttcatcact ctgcatagga gactgattgg gaatcatgac aactttttga
     4501 ccaacctcgt aggttccggt cgggtcagaa atgacggttc cacatgactc gtgaatcatt
     4561 gccattggaa gctttttatt caaaatcttg ggatcacgtt ttccctgata gtaacgctga
     4621 tccgcatgac agacagccat gtagttggga cggataagga tatgattctc ttggtcaata
     4681 gcctcttcct gatatttgac attgataaac ttaggcttag ttagttgata aatttgatta
     4741 atcattttac tagtctttct caatcatact ttttgcaatc ttcaaatctg ttacggttgt
     4801 aatcttcaga tttgagtatt cacctttggc caaggccaca tcttttcctt tgatcacaaa
     4861 gattttacat gcatctgtca agatttcctt ctcttcatca gaaagagatc cataaaggtc
     4921 catgaagtcc ttgcaacgga atgtttgagg tgtttgtcct tgataaaggt gagcacgatt
     4981 tggaatatct gtaataaatt gaccattggt actttcaacg atagtatcaa ccgcttctac
     5041 cactgtgtcc actgcgtcat gattttgggc aagttggata ctgtcctgaa tcatgcgaag
     5101 tgtaataaat ggacgaacag aatcgtgggt aacaacgata tcctctggag taagcggacg
     5161 ataagcatca atggcttcaa tgattttctt aatacttgta ttgcggtcag caccaccctt
     5221 tgtaatgatg atacgttcct tataaagagg aagatattta tctacaagat cttctgcatg
     5281 agaaacccag tctccatgaa caccaactac aattttttca atacttggct ccaagacaaa
     5341 tttttcaatt gtatgaatca aaataggtcg atcacctagc tctaaaaatt gttttggcaa
     5401 gttactgatc cccatgcgtg tgccagttcc accggcaaga attcctgcat aaatcatcta
     5461 ttttctcctt tgtcttacta aaaaaactta ttctctctat tataccacaa tctagtccta
     5521 tcataaaaga aatactggtc ctccccttct agaataggag aattatgcag ttctattatt
     5581 caaagaaact agtccatttc tagtaatgac taggaattct gtagcaatta ctaataaatg
     5641 gctgcgaaat tttagagtcc ttcagaataa tatactttcc ttaaagaaaa cttaaaactt
     5701 aagatttgaa acttcaaagt gattacttga cttactgccc ttcatttctt attagctaac
     5761 tttatgatat aatgaaaaac gaggtaaatt gaatgaaaag tataaaatta aatgctctat
     5821 cttacatggg aattcgtgtc ttgaatatta tttttcccat cctaactgga acctatgtcg
     5881 cgcgtgtctt ggaccgaact gactatggtt acttcaactc agtcgacact attttgtcat
     5941 ttttcttgcc ctttgcaact tatggtgtct ataactacgg tttaagggct atcagtaatg
     6001 tcaaggataa caaaaaagat cttaacagaa ccttttctag tcttttttat ttgtgcatcg
     6061 cttgtacgat tttgaccact gctgtctata tcctagccta tcctctcttc tttactgata
     6121 atccaatcgt caaaaaggtc taccttgtta tggggattca actcattgcc cagatttttt
     6181 caatcgaatg ggtcaatgaa gctctggaaa attacagttt tctcttttac aaaactgcct
     6241 tcatccgtat cctgatgctg gtctctattt tcttatttgt taaaaatgaa cacgatattg
     6301 ttgtctatac acttgtgatg agtttatcga cgctgattaa ctacctgatt agttattttt
     6361 ggattaaaag agacatcaaa cttgttaaga ttcacctaag tgattttaaa ccactctttc
     6421 tccctctgac agccatgtta gtctttgcca atgccaatat gctcttcact tttttagatc
     6481 gcctcttcct cgttaaaaca gggattgatg tcaacgttag ttactatacc atagctcagc
     6541 gaattgtgac cgttatagct ggggttgtaa caggtgcaat tggagtgagt gtgcctcgtc
     6601 tcagttacta tctggggaaa ggagacaaag aagcctatgt ttctctggtt aatagaggta
     6661 gtcgaatctt taacttcttt atcattccac tgagttttgg actcatggtt ttaggaccaa
     6721 atgccatcct actttacggt agtgaaaaat atatcggagg cgacatcttg acctctctct
     6781 tcgcttttcg tacgattatc ctggccttag ataccattct tggttcccaa attctcttta
     6841 ccaatggcta tgaaaaacgt atcacagtct atacagtctt tgctgggcta ctcaatttgg
     6901 gcttgaatag tctcctcttt ttcaaccata tcgtggctcc cgaatactac ttactgacaa
     6961 ctatgttatc agaggcttct ctacttgttt tctatatcat tttcatccat agaaaacaac
     7021 tcatccactt gggacatatc tttagctata ctgttcgata ctctctcttt tcactttcct
     7081 ttgtagcaat ttatttcctg attaatttcg tgtatcctgt ggatatggtc attaatttgc
     7141 catttttgat taatactggt ttgattgtct tgctatcagc tatctcttat attagtctac
     7201 ttgtcttcac aaaagatagc attttctatg aatttttaaa ccatgtccta gccttaaaaa
     7261 ataaatttaa aaaatcttag gagtttaaaa tgaaacaact aaccgttgaa gatgccaaac
     7321 aaattgaatt agaaattttg aattatattg atactctctg taaaaagcac aatatcaact
     7381 atattattaa ctacggtact ctgattgggg cggttcgaca tgagggcttt atcccttggg
     7441 acgacgatat tgatttatcc atgcctagag aagactacca acgatttatt aacatttttc
     7501 aaaaggaaaa aagcaagtat aagctcctat ccttagaaac tgataagaac tactttaaca
     7561 actttatcaa gataaccgac agtacgacta aaattattga tactcgaaat acaaaaacct
     7621 atgagtctgg tatctttatc gatattttcc ctatggatcg ctttgatgat cctaaggtca
     7681 ttgatacttg ttataaacta gaaagcttca aactgctgtc tttcagtaaa cataaaaata
     7741 ttgtctataa ggatagcctt ttaaaagatt ggatacgaac agccttttgg ttgctccttc
     7801 gactggtttc tcctcgttat tttgcaaata aaatcgagaa agaaattcaa aaatatagtc
     7861 gtgaaaatgg gcagtatatg gcttttattc cttcaaaatt taaggaaaag gaagtcttcc
     7921 caagtgctac ctttgataaa acaatcgatt taccctttga gaatttaagc cttcctgcac
     7981 ctgaaaaatt tgatactatt ttgacacaat tttatggaga ttatatgacc ctaccaccag
     8041 aagaaaaacg cttctacagt catgaatttc acgcttataa attggaggat taggatgcaa
     8101 tatttagaaa aaaaagaaat taaagaaatt caactagccc tgctggacta tattgatgag
     8161 acttgtaaga aacatgatat tccttatttt ctcagttatg gaaccatgct tggagccatc
     8221 cgccacaaag gtatgattcc ttgggatgat gatattgata tttcccttta tcgtgaggat
     8281 tatgagcgtt tactgaagat tattgaagaa gaaaatcacc ctcgctacaa ggttctttcc
     8341 tacgatacat cttcttggta cttccataat ttcgcatcga ttttggatac ttctactgtt
     8401 atagaagacc atgttaagta caagcgtcat gataccagcc ttttcatcga tgtcttccca
     8461 attgatcaat ttacagactt gagcattgtc gacaagagct ataagtatgt ggcccttcgt
     8521 caactagctt acatcaaaaa atcacgagca gttcacggtg atagcaaact aaaagatttt
     8581 cttagattat gtagctggta cgctctccga tttgtcaatc ctcgctactt ttacaagaaa
     8641 attgatcaac tagtcaaaaa tgctgtaacc aacattcctc aatatgaagg aggagttggg
     8701 atcggtaagg aagggatgaa agaaatcttc ccagttgata cctttaaaga actgatttta
     8761 actgagtttg agggccgtat gttgcctgtt cccaaaaaat atgaccaatt tttaacccag
     8821 atgtatggcg attatatgac accaccatca aaagaaatgc aagagtggta tagtcatagc
     8881 attaaagctt atcgcaaaaa ctgattgagg gggattatac aaactactaa gatagaggtt
     8941 attcaaaaac ataattttag tagaaaatga aatacatatt cccacaataa aacgcatcat
     9001 atcaaggttt ttgaaaaacc ttgatatgat gcgttttata attttaaaga cttttttcta
     9061 tagtagattg aaataagatg cgaacaaatc aattagaaaa ttcaaattaa tttctagaaa
     9121 tattttagta ttcctgtgta ctgttctaaa ttcagtctgc tatatcttat ttttctattt
     9181 aaatcgcttc tgtaacaaag ctacggcttt caagtacctt aagcatggca ttagctgtat
     9241 ctagcgctgt gaagaggggc accccgtgtt caatggctga acgacgaatt tgctcaccat
     9301 cttcgtcagc agttcgtttt gttcctactg tgttaatgat agcttgaatt cttcctttgc
     9361 gtacaaaact tgggatatcc ttatcgtcat caccaatctt accaacaggt tgggcttgca
     9421 agccatggct agcaaagaag gctgctgtcc cttctgtcgc aaggattcca taaccaatgt
     9481 tttggaaacg acgagccaag ttcaaggctt cttctttggc atcatcagcg atggtaaaga
     9541 cgacattacc aaaagttggc aagtgtagat aagaagcttc aaaggcttta tagagagctt
     9601 tttccaaagt agcatcagaa cccataactt cacctgttga cttcatttca ggaccgagca
     9661 agctgtctac cttagctagt ttggtaaagg agaagacagg tgccttgata tgaacacggg
     9721 tgctttcagg gtaaagtcca ttttggtagc caagttctga taaactttga ccaagaatga
     9781 gtttggtcgc tacttgagcc ataggaatat tggttacctt agataggaat ggaacagtac
     9841 ggctggcacg tggattgacc tcaataacgt agactttttc atccttgata acaaactgga
     9901 tgttcatcat tccaaggcag tgaagaccga ttgctaagcg tttggtgtag tctgcgatgg
     9961 tctcctgaac cttttgcgac aaggtttgtg gtgggtaaac agccattgag tcacctgagt
    10021 ggacaccagc acgttcgata tgctccatga taccaggaat gagtacattt ttaccatctg
    10081 aaatggcatc aacttcgcac tcttgcccaa cgatataaga gtcgacaaga actgggtggt
    10141 ctggactagc cttaacagca gttcgcatgt aagaacgaag gtcttcttcg ttttcaacga
    10201 tttccatggc acgtccacca agtacataag atgggcggac aagaactggg aagccaatct
    10261 tgcgagctgc aagagctgct tcttcttcat tggtagctgt ttgtcctggt ggctgtggaa
    10321 tatccaattc tttgagagct tgctcgaaga ggtcacggtc ttcggcacga tctaggtcag
    10381 caacctgtgt accaaggatg gtcacacctg cttttgccaa tggctccgca aggttgatgg
    10441 ctgtttgacc accgaactga acgataactc cctttggttg ttccaagtca atgacgttca
    10501 taacatcttc gaatgtcaat ggctcaaagt aaagcttatc tgatacagag aagtctgttg
    10561 aaacggtctc tgggtttgag ttcatgatga tagcttcata accagctgcc tggatagcct
    10621 taacagagtg aacggttgcg tagtcaaact caaccccttg accaatacgg attggacctg
    10681 aacctaggac aagtacagat tctttatcag atctgataga ttcattttcc caaccatagg
    10741 ttgaatagaa atatggcgtt tcggagtcga actctgccgc acaagtgtct accatcttat
    10801 aaactggaac aatcttgttt tccaagcgaa gttggcgaac ttgatcatca gtcgttcccc
    10861 agagttcagc aatcttacgg tctgaaaaac cattaagttt ggctgttttc aaaacttcta
    10921 gatcttgtgg atgagcaccc aattcttgct caatttcaaa gatatgcaag agtttatcaa
    10981 gatagaagat atcaattttt gtaagctctg caatttcttc aggtgtgtag ccacgacgaa
    11041 ttgcttctga tacgtagaag agacggtcat cttgggcttt gacaaccttt tcaatcaagg
    11101 catcatcaga aactgctgca agttcaggta tttcattgtg gtgcacccca atttcaaggg
    11161 agcggcaggc cttgagaaga gattcctcga tgttacgacc gattgccatg acttctccag
    11221 tcgccttcat ttgtgtaccg agacggcgtt cacccttttc aaacttgtca aatgggaaac
    11281 gtggaatctt agcaactacg tagtcaaggg ctggttcaaa catggcatag gttgaacctg
    11341 taactgggtt tataacctca tccaaggtca aacctactgc aatcttggca gccaacttag
    11401 caatcggata tcctgtcgct ttagaagcaa gggctgacga acgtgataca cgagggttta
    11461 cttcgataac ataatacttg aagctgttag gatcaagagc tagctgaaca ttacatccac
    11521 cttcaatctt gagggcacga ataatgctca agctcgcatc acgaagcatt tggttttcat
    11581 agtctgacat ggtttgcgca ggggcaaata caatggaatc ccccgtgtga atcccaactg
    11641 ggtcaaagtt ttccatgtta caaacaacca aggcattgtc agctgagtca cgcatcactt
    11701 cgtattcaat ttccttgaaa ccggcaatcg aacgctcaat caaacattgg gtaacaggtg
    11761 acaatttcaa accattttca gtgatttcac gcaattcttt ctcgttggca cacataccac
    11821 caccagtacc accgagggta aaggctggac gaacgatgac tgggtagcca attgtcgctg
    11881 caaaggcaac tgcttcttct actgtgttaa caatttcaga ttctggaatg ggttgttcaa
    11941 gctcttccat caattgttta aagaggtcac ggtcctccgc ttggtcaatg gcagataatt
    12001 tggtacccag aagttcaacg ccaagctcat ctaagatacc atttttagac aattccatgg
    12061 ccatgttgag acctgtctga ccaccgagtg ttggtagcaa ggcatctgga cgttccttac
    12121 gaagaatacg tgtcacaaac tcaagtgtaa tcggttcaat gtaaaccttg tcagcaattt
    12181 ccttgtccgt catgatggtt gcaggatttg agttaaccaa aacaacctca taaccttcct
    12241 ctttcaacga caagcaagcc tgagtcccag cgtagtcaaa ctcagcagcc tgaccaataa
    12301 taatcggacc agaaccaatc accataattt tttgaatatc agtacgttta ggcatagttt
    12361 atgatacaaa gcccgtaaag aacacagtga aaataggaaa ctcggcgcag acgccttcag
    12421 cgtcaagacg atgtttatct ttttcacaca gttcttaggg cgtgttcact tccgcgaaca
    12481 atgtatcttc tcctttctat tcgtcgcctc tcagggcgac attaaataag atacaaagga
    12541 cgactagaaa gcgattgaat tttaggaaat caaggaagga ttgacaatcc aagttggttt
    12601 ctctaaattc tgagctttcc gtccatgttc agttacataa attctccgac gagcttttac
    12661 tcattcttag tttgattgtt taaaaacttc catcatctcg ataaactcgt caaataggta
    12721 gctagcgtcg tgtggcccag gagctgcatc tgggtggtat tgaacagaga aagcaggttg
    12781 gtatctgtgg cgcacacctt ccactgactt gtcattgatt tcttcgtggg taataatcaa
    12841 gtgctctggc aaatcctcgc ggctgactgc ataaccatgg ttctggctgg tgaagtctac
    12901 tcgtcctgtt gcgatttcac gtaccgcatg gttgaatcca cggtggccaa acttcatctt
    12961 ataggtctta gccccgtttg ccattgcaaa gagttggtgt cccatacaaa taccaaagat
    13021 tggaattttt ccttgtacac cgcgaatcat gtcgagtgct tgtggaacgt cttctgggtt
    13081 acctggacca tttgacaaca taactccatc aggattgaga tggagaattt cttcagccgt
    13141 tgtcgaataa ggaacaactg tcacgttaca gttgcgttta gaaagttcac gtaggattga
    13201 gtgcttgaga ccaaagtcca ctagcaccac gctcaaacca actcctggag ctggataaga
    13261 cgttttagta gaaacctgtt tgatattgtc tgtcggtaaa actgttgctt ggagctggtc
    13321 cgtcacatgg tccatactgt ccccaacatg ggtcaaggtt gcacgcatag taccatgctt
    13381 acggataatc ttggtaagag cacgcgtatc aattcctgaa atccctggaa ttttcttggc
    13441 tttcaaaaat tcatccaagg tcatttggtt gcgccagttg ctagctctac gcgcttcttc
    13501 aaaaacaacg actcccttac aagttggaat aatggattca taatcatcac gattaatacc
    13561 ataatttcct accaaaggat aagtaaaggt caagatttgt ccattataag actggtctgt
    13621 aatggattct tggtagccgg tcatccctgt attaaagacg atttcgcctg ttacatcaat
    13681 atctgctccg aaggccttgc cttcaaaaac tgtgccatct tctaatacta gaattctttt
    13741 tgtcatattt tcacctctcg tggacgctca ctggcgtctt ttaacgtctt gtgttttagt
    13801 tggcgtttct actcgctagt acggattcta agattgccat tcgaacaaag acaccattgg
    13861 tcatttgttg gacaatccgt gattttggtg cttcaaccaa gtggtctgct atttctacat
    13921 cacgattgat tggagctggg tgcatgagga ttgctgtttc tttcaaacga tcgtaacgtt
    13981 cttgagtcaa gccatgttgg gcatgatagt cttcttttga aaatacagct ccactatcat
    14041 ggcgttcgtg ttgcacacgg agaaacatca tgacatcaac ctgatcaatg atttcatcaa
    14101 tggttacaaa ctttccatag tctgcaaact cttgacttct ccattcctca ggtccagcga
    14161 aaaagagttc agctcccaag cgtttcaaaa tctgcatatt ggatttggca acgcgtgagt
    14221 ggtccaagtc acctgcaata gcaactttaa gaccctcaaa gtggccaaat tcctcataaa
    14281 tggtcatcaa atcaagcaag ctctggctag ggtgttggcc cgaaccatct ccaccattga
    14341 tgatggaagt cgtaatcgtt ggactagcaa tcaattctct atagtagtcg acctctggat
    14401 ggcgaatcac acagacatcc actcctaaag cagacagagt caaaatggtg tcataaagtg
    14461 tctcaccctt attaaccgaa ctagtcttca catcaaagtc aagtcgttcc aatccaagtt
    14521 taatctctgc gacttcaaag gacttatgtg tccgtgtaga atcctcaaag aagagattgg
    14581 aaacaatcgg atggtcttca tagggaagct gggctccatt tttaaactca attcctcgct
    14641 tgatcaattt cattacttga tcgacagtga ggtcttccat ggacaccaca tggttcaatg
    14701 cttgttgatt ttctgacatg gctactcctt taactttcta agcttcttca gtaatcagaa
    14761 ctctgtcttg gtcatcaagt tctgtcatct ctacgatgat ttcttcagaa cgactggttg
    14821 ggatattttt tccaacgtaa tctggacgga ttggcaattc tctatgtcca cgatcgacta
    14881 gaactgctaa actcacacgc gcaggacgac catgaccgac aatattatca atagcagcac
    14941 ggatggtacg acctgtatag agcacatcat ccaccaagat aacttcgcgg tctgtcacat
    15001 cgacagaaac caaagaagta tcttctccac ttttaacatc atcacggaaa ggtttagtat
    15061 ccaattccac aacaggaact gaaagatttt ctaactgctt caaacgttct tggattcggt
    15121 gggcaataaa gacaccacga gttttaatac cagccaagac gatcttattc aaatctttgt
    15181 tgcgttcgat aatctcataa gtaatacgcg taatcgctcg tttgacggtc aattcgtcta
    15241 caacttcttt tgttttcatg acaaacctcc aaaaagaaaa gtctccttaa acaaggagac
    15301 ttgaaattta tagccaagcg agccctactg caaacagtat agacttcacc cttctacttt
    15361 atcgcgctcc ttgcctgcct cacgggacag gtttaaagga atatttagtt atcatttact
    15421 atagcacaaa gcatgcttaa aatcaagcaa aaagtttcaa tgtagcatct tacaaattgc
    15481 taaaatcata taattgtggg tactggtcac actctggatt ttttggatgg caaatggctc
    15541 ttccaaaata aatcatggcc tgatgggcag ctaaccactg ctcaggcggc aagatatcca
    15601 tgacccgctt ttccacctca agtggcgtcg ctgatttttt gacaatatcg tggtgtttgc
    15661 aaatacgctc cacatgagta tccactgcaa aggctggaat cccaaatcct acactcatga
    15721 caacattggc tgtcttgcga ccaacacctg ccaaactctc caattcttca cgtgtctgag
    15781 ggacttgacc atcaaaatcg tctagtaact gttgggcaca ttttttaagg aatttagctt
    15841 tattccgata caatcccaag cgagaaatat gtgaagcaat ctcactctct gtcgctacag
    15901 acatagcttg gggtgttgga aaggcaacaa agagacctgg tgtggcctta tttaccgctg
    15961 catctgtcgt ctgggctgat aacatgaccg caaccaggag ttcaaaatga ttggtaaaat
    16021 caagactagg cttggcatct gggaagaggg caatgatttc ttctagcacc tttcgtgctc
    16081 gtttttttga caagaccatt attcatctcc gtcaaatagt ccttgtaagc cagcaaaagg
    16141 actgttttct tctttcttta ctgcttgttg agcttggtat tcttcctctg tcatgatttg
    16201 ccagtcattt cctgagataa atccttgacc agcttcttct tcagccgtca agaccttgat
    16261 aggaatgttt agcaggatat tgtctgatac actctcagca aggtcaagct ccccattttc
    16321 aatgggcaag accaagtcat catctaaaac ttcttgatct agctggttag ttgcgccttc
    16381 catgaaaact tccgtgactg gataagattc aactaactca actggctcca tactgcgact
    16441 cgaagcaaga acaatggtat aagatagttg ataatctaag aaatacatac ggtcttcata
    16501 ttgtactttc ccaactgcaa ggatatcttt tacatctaaa atttcttgat tacgtgcacg
    16561 caggtcatca actaaatcta acgtttgttc aaagttcaaa ccttcagact gcttacgaat
    16621 ttcttgaata tttaatttca tacttcctcc ataaagattt actctcttga ttataccatg
    16681 aaaaggctac aaatcagcac accaaacttt gtaattaaaa ttcaaaattt taacatattt
    16741 actatgacag ttttattttt tagtgctata ctatagggaa agagtacatc agatcaagga
    16801 ggatgctcat atggaagaca tggaagacaa gaaactcatt caactcctat ccaagttaaa
    16861 taaaagctac caaaactgta aacagggtac ggcagatgat attcgactac aagagctgct
    16921 aaacactact atgcaagagc tcaaaaaaac ggaaaagttg aacaacagta tcttaattga
    16981 tcttgagaaa ttttaccaac ccaccagtcc tctgattgga ctgggtagcc taaaactaaa
    17041 cgatcaagca cgcactgctt ggcgaaacta tgataaaatc cattacgagc atgtcaaaca
    17101 cgtactaagt ctctatggac ctgtttttgg attttagagc atagaatttc cagttttctg
    17161 ttgacaaaat ttccttaaaa gtatagtata aagatactaa tactcggagg taagggagac
    17221 atgaacaact aagtctatca aataaagaac ctttatttag tagatcttgt ttttgtctct
    17281 ttttgtgtgc tcttttatgc tctttttctg gcatgttaat agagtttttt tgacatagac
    17341 tttgggctct actaggtaaa gtagagcttt ttgttatgca ctatcgacat tctagaaagg
    17401 gcaatagtat gataaaaatc aatcacctga ccatcacaca aaacaaagat ttacgagacc
    17461 ttgtctctga cctaagcatg accatacaag acggggaaaa ggtagctatt attggcgaag
    17521 aaggaaatgg taaatcaacc ttacttaaaa ctttaatggg ggaagatttg tctgatttca
    17581 ctatcaaggg agacatccag tctgattatc agtcactggc ctacattcct caaaaagtcc
    17641 ctgaggacct aaaaaagaaa actttacacg actacttctt tttagattct attgatttag
    17701 actacagtat cctctatcgt ttggcggagg aattgcattt tgatagcaat cgtttcgcaa
    17761 gtgaccaaga gattggcaat ctatcagggg gcgaagcttt gaaaattcag cttatccatg
    17821 agttagccaa accctttgag attctatttt tagatgaacc ttcaaatgac ctagaccttg
    17881 agacagttga ttggctaaaa ggccagattc aaaagaccag gcaaaccgtt attttcattt
    17941 cccatgatga agactttctt tctgaaacgg cagacactat tgttcacttg cgactggtca
    18001 aacaccgtaa agaagcggaa acgctagtag agcatttaga ctatgatagc tatagtgagc
    18061 agagaaaggc taattttgcc aaacaaagtc agcaagctgc taacaaccaa agagcctacg
    18121 ataaaaccat ggaaaaacat cggagagtta agcaaaatgt agaaactgcg cttcgagcta
    18181 ccaaagatag tactgccggt cgcctattgg ctaaaaagat gaaaactgtc ctctcacaag
    18241 aaaaacgcta cgaaaagaca gctcagtcca tgactcaaaa gccacttgaa gaggaacaaa
    18301 tccaactttt cttttcagac atccaaccat taccagcttc taaagtctta gtccaactgg
    18361 aaaaagaaaa tttgtccatt gacgaccgtg ttttggctca ggaactgcaa ctaactgtcc
    18421 gtggccaaga aaaaatcggt atcatcggcc caaatggtgt tgggaaatca actctgttag
    18481 ccaaattaca gcaacttctg aatgataaaa gagagatttc acttggtttt atgccacaag
    18541 attaccacaa aaaactgcaa ttggatttat cctcaatagc ttatatcagc aaaactgggg
    18601 aaaaagagga acttcagaaa atccaatctc acctagccag tctcaatttc agttatccag
    18661 aaatgcagca tcaaattcgc tccttatctg gcggacaaca gggaaaactc ctgcttttgg
    18721 atctagttct gcgcaaacca aactttctcc tgctggatga acccacacga aacttttctc
    18781 ccacttctca accccaaatc agaaaactct ttgcaaccta tccaggcggt ctcatcactg
    18841 tttcgcatga ccgtcgtttc ttaaaagaag tctgctcgat catctatcgc atgacagaac
    18901 acggtttgaa gctagttaat ttagaagatt tataaatttg caacatagca aaaatccaga
    18961 gacgacctct ggattctttt acatctgttt taaacgttca atccgttctg agataggtgg
    19021 gtgggtataa aagagttttt ggaacccccc acctttctta ggatcattga tataaagggc
    19081 actgctagca tcatcgacgt ggcgactcat aggtttgcta ttgtccaact tatctagggc
    19141 attaatcatt ccctggggat tgcgagtcag ctcgacacta gatgcatctg ccagaaattc
    19201 cctctgacga gaaatagcga gctgaaccaa ggttgcagcg agaggtgcca gtacaatagc
    19261 tagtagggaa accactagca taatgatttc aagaccattt ccatctcggt catcatcact
    19321 tcgtctgcga cctgctccac cccaccacat catacgacct gccatactag aaagcatggt
    19381 gatagcacta gcaagggcaa ctgcaatagt cgaaatacgg atatcataat tacgaatatg
    19441 actgacttca tgtcccataa cagcttctag ttcttcacga ttcatgatag ctagtagacc
    19501 tgaagtcgca gcaacagccg cattttgagg attagaacct gtcgcaaagg catttaaggc
    19561 tggatcatca atgatgaaaa cacggggcat aggaatctga gcgaccagag ccatatcttc
    19621 cactacatgg tagaggtctg gtgccgtttg ctcatccacc tcacgcgctc cattcatgga
    19681 catgacaatc tctgtcgatt gaaaaatcat agacaaagcg tagataaagc cgataatcag
    19741 tgcgataacc aaaccaccaa gtccagatct tataaagaga taaccaaccg cataaccaac
    19801 aagagctaag agtaggaaaa ataccagcaa caaaatccag gtttttcgtt tattgcttgc
    19861 aatttgatca aacaacatct tagtcaccta aaccgctaaa atcaacttta ggaaccgact
    19921 tttcctcttc agatgtttga aggaaatctg ccgctttaaa tccaaacatt ccagcgataa
    19981 tattgctcgg gaaagtttct aattttacat tgtagttgct gacaacactg ttatagagtt
    20041 gacgagagta agaaatttta ttttctgtgt ttgtcaactc ctcttgcaat ttaacaaagt
    20101 tagcactagc tttcaaatct ggatagcttt ctgcaactgc aaaaatacct gaaacctgac
    20161 gagtgagggc atcactggct ttcatagctt ctgctggtga agtcgctgcc gccacttggt
    20221 tacgtagttc tgccaccttt tcaagggtag aaccttcata tttggcataa ccttttacag
    20281 tctcaatcaa gtttggcaag aggtcatttc gacgtttcaa ctgaacatca atctgactcc
    20341 aagcctcctt ggtttgcata cgatttttaa ccaaaccgtt atagctaaca atcacaaaaa
    20401 taacaataag agcgataact ccaagaataa tccaagtcat aatataagtc ctttctgctt
    20461 ttagattagt accagtatat caaattttct atgattgtgg taaaataaga tgatactaaa
    20521 gaaggaaata actatgaaac caaaaacatt ttacaacttg cttgccgagc agaatcttcc
    20581 actttcggac cagcaaaaag aacaatttga acgttatttt gagctcttgg tcgagtggaa
    20641 tgagaagatt aatttgacgg cgattacgga caaggaagaa gtttatctca aacattttta
    20701 cgattcgatt gcacccattc ttcaaggttt gattcccaat gaaactatca aacttcttga
    20761 tatcggggct ggggcaggat ttcctagtct accaatgaaa attctctatc cggagttaga
    20821 tgtgaccatt attgattcac tcaataagcg catcaacttc ctacaactct tggctcaaga
    20881 actggatttg aacggagttc atttctacca cggacgtgcc gaagattttg cccaagacaa
    20941 gaacttccgt gctcaatatg attttgtaac agctcgtgcg gttgcccgta tgcaggtcct
    21001 atctgaattg accattccct accttaaggt tggtggaaaa ctattggcac tcaaggccag
    21061 caatgcgcct gaggaattat tagaagctaa gaatgccctc aatctccttt ttagtaaggt
    21121 cgaagacaat ctcagctacg ccctaccgaa tagagatccg cgctatatca cagtggtaga
    21181 aaagaaaaaa gaaacaccaa ataaatatcc gcgtaaggct ggtatgccaa ataaacgccc
    21241 actttaaatt ttttagtaaa caaatgttta caaaatcagc ctcgctcttt tatttctagg
    21301 ctcgggaaaa aatgatttac aaaatcattt ttttctgcta tactatccta agcaaaggtt
    21361 tttaatgtca tcccgtgagg tgacgaagac gcagaaatat ttaaaactct ttaaaatcta
    21421 aattttaaag aagtcttact ctgagggcct attgctgtaa aataatgggc tcttttttga
    21481 tgcccaaaag tgaggtttat atgaaacaag aatcaactgt tgatttgtta ctagacgttg
    21541 accaacgtcc ttcggctgga aaaggaattc tccttagttt ccaacacgtt ttcgccatgt
    21601 ttggtgcgac catcttggta ccattgattt tgggaatgcc tgtatctgtt gccctttttg
    21661 cttcaggtgt tggaacactc atctacatga ttgctactgg ttttaaagtt ccagtttatc
    21721 taggttcttc atttgccttt atcacagcta tgtcactggc tatgaaagaa atgggggggg
    21781 atgtatctgc tgcccaaaca ggggttatct tgactggttt ggtctatgtc cttgttgcta
    21841 ccagcatccg atttgtagga acaaaatgga ttgataaact cttgccacca atcattatcg
    21901 gtcctatgat catcgttatc ggtcttggac ttgcaggttc agctgttacc aatgcaggtc
    21961 ttgtagcaga cggaaattgg aaaaatgctc tggtagccgt tgttactttc ctaattgctg
    22021 cctttatcaa tacaaaagga aaaggcttcc tacgaatcat tccattcctc tttgccatta
    22081 tcggtggtta ccttttcgca ctaactcttg gcttggttga ctttacacca gttcttaaag
    22141 ccaactggtt cgaaattcct ggtttctact tgccatttag cacaggtggt gcctttaaag
    22201 agtacaatct ttactttggt ccagaagcca tcgctatctt gccaatcgct atcgtaacaa
    22261 tttctgaaca tatcggagac catactgttt tgggtcaaat ctgtggtcgt caattcttaa
    22321 aagaaccagg tcttcaccgt actcttcttg gtgacggtat cgcaacttct gtttctgcct
    22381 tccttggtgg accagccaat acaacttacg gagaaaatac aggggttatc ggtatgactc
    22441 gtatcgcttc tgtctcagtt atccgtaacg ctgccttcat cgcgattgcc ctcagcttcc
    22501 ttggtaaatt cactgccttg atttcaacta ttccaaacgc tgtacttggt ggtatgtcaa
    22561 tccttctcta tggggttatc gccagcaatg gtttgaaagt cttgattaaa gaacgtgttg
    22621 atttcgctca aatgcgaaac ctcatcatcg caagtgctat gttggttctt ggacttggag
    22681 gagctatcct taaacttggt ccagttacac tttcaggtac tgccctttca gccatgacag
    22741 gaatcatctt gaacttgatc ttgccatacg aaaataaaga ctaagagtct aaatacacct
    22801 aatccactca gacagctgag tggatttttc gtataccata ataaaagtgt cttaacaaaa
    22861 ttattaaaat caaaaaacgt ataatatcag atattctaaa accttgatac tgtacgtttt
    22921 atcatagaaa tttttacttt attttctcat caaatgagat ttgcatcaat ctcttgtctt
    22981 acttgcgttt cttcttcgct ttcttcattt tgttagccat acgtttcatg gactgtttca
    23041 tggcaaattc accaatttta cctttcaaac cgccaccaaa catctggctc atatctggca
    23101 ttcctgctcc tccgagagct gataagtcag gcataccgcc ttgtcccatc attccttcaa
    23161 gggcagacat atccattcct cccatatttg gcatattttt aggaaggtta tttggattaa
    23221 tccccatttg cttcatcatt ttattcatat ccccagacat aacaccctgc atgagctgtt
    23281 tagcctggtt aaagtccttg atgaatttat tgacttcgac gaatgtattt ccagaaccag
    23341 cagcaatacg acggcgacgg cttggattta acaaatctgg gttttcacgc tcttcaggtg
    23401 tcatcgaaga cacaatggca cgtttacgag caatctggcg ttcatccacc ttcatgtttt
    23461 gaagggctgg attgttggcc atacctggaa tcatcttgag caagtcttcc atcggcccca
    23521 tattttgcac ctgatctaat tgatcgatga aatcattaaa atcaaaggtg ttttcgcgca
    23581 tcttctcagc catttcaagg gctttttgtt catcgtattc ctgagaagct ttctcaatca
    23641 aagtgagcat atcccccata ccaagtatac ggctagacat gcggtctggg tggaaggttt
    23701 caatgtccgt aatcttttca cctgtaccag tgaacttgat tggttttcca gtaatgtgac
    23761 gaacagacag agcagcacca ccacgagtat cgccatcaat cttggtaagg atgaccccag
    23821 tcacttccaa ctgagcatta aactcacgcg caacattggc tgcttcctga ccaatcatag
    23881 catcaacgac aagcaagatt tcatttggtt gagccaatgc tttcacatca cgaagctcat
    23941 tcatgaggag ctcatcaatc tgcaaacgac ccgcagtatc aatcaagaca tagtcgttat
    24001 gattagtttg ggcttgctcc aaaccttgac gtacaatctc aacagctggt acttctgttc
    24061 caagtgcaaa gacaggcaca tcaatctgtt gtcccaaggt cttaagctgg tcaatggcag
    24121 ctggacgata aatatccgcc gcaatcatca aaggacgagc attttcttct ttcttgagtt
    24181 tgttggccaa tttaccagca aaggttgttt taccagcccc ttgtaaacca accatcatga
    24241 tgatggttgg aatcttaggt gacttgataa tttctgccgt atcagaacct aaaacggctg
    24301 tcagttcctc atcaacgatt ttaataatct gttgcgcagg attaagtgta tcaatgacct
    24361 catgcccgac tgcacgctca cgaactttct tgataaagtc ctttacaaca ggcaaggcaa
    24421 cgtcggcctc gagcaaggcc aagcgaattt ctttggttgc ctcttggaca tcagattcag
    24481 agatttttcc ttttttacgt agatttttaa agacgttctg caaacgttct gttaaacttt
    24541 caaatgccat ttttcttcct cttattctct attatcaatg cttgttaaaa tttctatctg
    24601 ctcctgcaga aagtcatcct tgggatagcg ctccaaaatc tgatcaaaaa tctgactgcg
    24661 gacaatatag tccgagtaca tgtgcaattt catctcataa tcttccagaa tcttttctgt
    24721 tcgcttgata ttgtcataga cagcctgacg actgacacca aactcctcgg caatttcagc
    24781 aaggctgtaa tcatcagcgt agtagagctc gatataattc atttgcttat ctgtcaaaag
    24841 cgccgcataa aattcaaaga gcgcattcat acgattggtt ttttcgattt ccataacttt
    24901 tattatacca aaaattagcc taatctacca cactaggaag ccgatccaag aagatagata
    24961 gctaaatttg aaaaagacat gagcctagcc ccaagtaatt tccaattgat agctggcaaa
    25021 aggatgtccc tcttgatttt gtagttgata atctagttca atcttttgcc tatcaacttg
    25081 ataatggctc gtttggatga taaactcctg catgcccata ggtgtaggaa tataggctaa
    25141 actatcgcta tcctttagaa agcgcataat ggtcttggga ttagaaaatc ggctcatcat
    25201 aagttcttga ccatgaaatt taatcactac tttttccttt tcctcattat agaaaagcag
    25261 gtagctataa tctccttttt catgcacttc cacatcataa agctggtcaa tcacttccaa
    25321 ctgctcatca aactgaattg tatttcgcat ccgaatcttc acatcaggcc ctctttcttg
    25381 tctcttgtcc tactatttta ccaaaaagag caggattttg ctataatggt catatgaacg
    25441 aaaaagtatt ccgtgaccca gttcacaact acatccatgt caataatcaa atcatctatg
    25501 acttgattaa tacaaaagaa tttcagcgtt tgcgccggat caaacaactg ggaacttcca
    25561 gttatacctt ccacggtgga gaacacagtc gcttctctca ctgtctagga gtctatgaaa
    25621 ttgcacgacg catcacagag attttcgaag aaaaatatcc tgaggaatgg aatcctgccg
    25681 agtctctctt gaccgtgacc gctgctctcc tacacgacct tgggcatggt gcctactccc
    25741 atacttttga aaatctcttt gatacagacc atgaagccat tactcaggag attattcaaa
    25801 atcctgagac agagattcac caagtcctgc tacaagtggc acctgatttc ccagaaaagg
    25861 tggccagtgt cattgaccat acctatccta ataagcaggt cgtgcagctc atttctagtc
    25921 agattgacgc agatcgcatg gactatctct tgcgcgactc ctattttaca ggagcatcct
    25981 atggggaatt tgacctgact cgcatcctcc gagtcattcg tcctgtcgaa aatggtatcg
    26041 cctttcagcg caatggcatg cacgccatcg aagactacgt cctcagtcgc taccagatgt
    26101 acatgcaggt ttatttccac ccagcaacac gcgccatgga agttctccta cagaatcttc
    26161 tcaagcgcgc caaggaactc taccctgagg acaagaattt ctttgcacga acttccccac
    26221 atctcctgcc tttcttcgaa aaaaatgtga ccttgactga ctatctggct ctggatgatg
    26281 gcgtgatgaa tacctacttc cagctttgga tgactagtcc tgacaaaatt cttgcagatt
    26341 tatctcaacg ctttgtcaac cgcaaggtct ttaaatccat taccttttca caagaggacc
    26401 aagatcaact tactagcatg agaaaattgg ttgaggacat cggctttgat cccgactact
    26461 atactgccat tcataagaac tttgacctcc cttatgatat ctatcgtccc gaatctcaaa
    26521 atccacggac acagattgag attttacaaa aaaatggaga actggccgaa ctctctagcc
    26581 tgtctcctat cgtccaatcc cttgctggca gtcgccacgg agataatcgt ttctatttcc
    26641 ctaaagaaat gttagaccaa aacagcatct tcgcaagcat tacccagcaa tttttacact
    26701 tgattgagaa cgatcatttt accccaaata aaaactagaa gaggaatttt atgagtatta
    26761 aactaattgc cgtcgatatt gacggaaccc tagtcaacag ccaaaaggaa atcactcctg
    26821 aagtcttttc tgccatccaa gatgccaaag aagctggtgt caaagtcgtg attgcaactg
    26881 gccgccccat cgcaggtgtt gccaaacttc tggacgactt gcagttgaga gaccaaggtg
    26941 actatgtggt gaccttcaat ggtgcccttg tccaagaaac tgctactggc catgagatta
    27001 tcagcgaatc cttgacctat gaggattatc tggatatgga attcctcagt cgcaagctcg
    27061 gtgtccacat gcatgccatt accaaggacg gtatctatac tgcaaatcgc aatatcggaa
    27121 aatacactgt acacgaatca accctcgtca gcatgcctat cttctaccgt acccctgaag
    27181 aaatggctgg caaagaaatt gttaaatgta tgtttatcga tgaaccagaa attctcgatg
    27241 ctgcgattga aaaaattcca gcagaatttt acgagcgcta ctccatcaac aaatctgctc
    27301 ctttctacct cgaactcctt aaaaagaatg tagacaaggg ttcagccatt actcacttgg
    27361 ctgaaaaact cggattgacc aaagatgaaa ccatggcaat cggtgatgaa gaaaatgacc
    27421 gtcccatgct ggaagtcgtt ggaaaccccg ttgtcatgga aaatggaaat ccagaaatca
    27481 aaaaaatcgc caaatacatc accaaaacaa atgacgaatc cggcgttgcc catgccatcc
    27541 gaacatgggt actgtaaaag tatcattttt caataagaat tgattagcaa taaaatccaa
    27601 tgaatttttt tagcaaacta tttaatttaa aacaaaataa tcataataga gacacaaatt
    27661 ctgattgtaa caatttttac ctaaacgaat tagaatgtgg ccttactcct gggcaactca
    27721 tactcataga ttggactcaa aaaacaggga gaaattataa tttcccaaga tattttaaat
    27781 actctcttca aattgaccct gaatctacac acaatcaatt atacaaatta ggatacttca
    27841 ctaaaaataa gactttatca tatcttacag tagtagaatt aaaaactata ttatctaaac
    27901 ataatttagc tacttctgga aaaaaagcag aattaattac aagaataatt aataatgtta
    27961 acattgacaa tttagatatt ccgttcgaat ttaaactaac aaaagaagca caaaatctta
    28021 ttatcgaaca tagtgactat atcaaagcat actatgataa agacataagt atggaagatt
    28081 attgtaaaga aaaaaacaat atctctttta aagcaacttt tggtgatata aaatggagtc
    28141 tcttaaataa acaagctcat aggaatactg tatcaggaga ttttggatgc ttatctaaca
    28201 cacgaaaggc tcagggaaga catttggaac aagaaggtaa tattaaacat gctttaatat
    28261 attacataga atctttgata attactattt caggattaga aaacaatttt tcagccactg
    28321 attatccagt atattatccc gattcgatac ctgactactc actaaaacat attcaaacat
    28381 taatggaatc attatctgat gacgattatg attttgcttt tgatgaagca ttatttcgct
    28441 tctcaatttt gaatgcaaat cattttttat ctaaggaaga tattgactat ttaagagtta
    28501 atttacctcg ttccactgct gaagaaataa acaattactt aaagaaatat gaatgttata
    28561 gtcctttaaa taatttagaa cttgacgatt ttgaataaat tgactataca aacatttata
    28621 tactcgatat agtctcaatt ttatctgatg attgcccaaa tttttcaata ataaaacgca
    28681 taatattatg gagacaatcc cctatattat gcgttctttt aatatcaaag actctttgac
    28741 aaacttcttt gatatctaat tacatgcccc ctgcaggaat cgaacctgca actactcctt
    28801 aggagggagt tgttatatcc attgaactaa gggagctaga taaaaactct gctgaaaaag
    28861 cagagttttt tagtcgaatt aacgacggat ttctttgata cgagctgcct taccttgaag
    28921 agcacgcaag tagtacaatt tcgcacgacg tactttaccg taacgtacaa cttcgatttt
    28981 ttcaacacgt ggagtgtgga ttgggaagat acgctcaaca cctacaccgt tagagatttt
    29041 acgaactgtg tagttttcag agatgccagc acctttacgt gcaataacaa caccttcaaa
    29101 aatctggata cgttcacggt taccttcgac aactttcgca tgtacacgaa cagtgtcacc
    29161 aggacggaat gatgggatat ctgtacgaag ttgaccttca gtcaagcttt ggattaatgg
    29221 attcatttta ttctcctatc tttgtcaatc ttgaggaatc ttcctcagcg gataaactgt
    29281 atttttgtgc gtccattaca cacaagatat agtttaccaa atttctaaaa aaaagtaaag
    29341 aaatttatag caaaattcct aaaaaaatcg caaccaaacc acctatgtaa gtcaaagtca
    29401 ggtaagaata aaagaccttc ttatcactta gcagtctttg aagttcgtca ttcaaggtcg
    29461 aaaaagttgt taaaccgcca caaaatcctg ttgctagaat agcatatact tccttagact
    29521 ctacatgatt gtagaatact ccaattaaaa aacaacctaa aagattagct atgagcgttc
    29581 cgagtggcaa tttagaagct tgattatagc gagaaaagaa ataccgtacg agtgctccca
    29641 agccacaggc gattgcaaga tagacgatta ccatttcttc ctccccaaat agtaagctaa
    29701 aagcaggcct ccaccgatac tcaaaagcaa atacaggact aaactaaaat aacgccctgt
    29761 atcaagcagc ttcacagtat caagcattag gctggaaaaa gttgttaaac ctccgcaaaa
    29821 acccgtcccc agtgctaaaa tcaagccctt actagtcccc ttatagacca gatagccctt
    29881 gacaagaaaa atcaagcaga aaattcccag atagttgaca aagagagtgc cccaaggaaa
    29941 gtctggactg gctggtaacc aggtggaaac tagatatcgg acaagtccgc ccaacatagc
    30001 agctagaaaa attcctagcg gataaaattg ttcttttttc atttgatatt ttgatcctga
    30061 taatcacgcg aacgtttgag tatgtacgaa aaagtcgcga caatagtctc ctgatagcgc
    30121 ttgtcctcta cacgactcct aaccttttca aaaataactt cttctctctt agtatctaaa
    30181 atcggtttac ctgaagcttt cttataagca accaccccct caactaaatg cattcgttct
    30241 tctaacagtt tgacaatttg gtcgtcgatt tgatcgattt cttgacgaat aatatctaaa
    30301 tccatacagt ctcctccttt atttgaatta ttgtatcaaa aagccatcaa ttaggctagt
    30361 aaaatcccaa ctctcgataa gaaaaatgca ctgattgatt acatcagcgc acttttat
//
LOCUS       DAVBCN010000020        28408 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVBCN010000020 DAVBCN010000000
VERSION     DAVBCN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28408
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.020"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            380..1333
                     /locus_tag="VQ497_000813"
     CDS             380..1333
                     /locus_tag="VQ497_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2517152.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLISLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            1384..2574
                     /locus_tag="VQ497_000814"
     CDS             1384..2574
                     /locus_tag="VQ497_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HET2517153.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGTDAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            2723..3094
                     /gene="rnpA"
                     /locus_tag="VQ497_000815"
     CDS             2723..3094
                     /gene="rnpA"
                     /locus_tag="VQ497_000815"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET2517154.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            3069..3893
                     /locus_tag="VQ497_000816"
     CDS             3069..3893
                     /locus_tag="VQ497_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET2517155.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAKIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            3912..4898
                     /locus_tag="VQ497_000817"
     CDS             3912..4898
                     /locus_tag="VQ497_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HET2517156.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            4949..5572
                     /locus_tag="VQ497_000818"
     CDS             4949..5572
                     /locus_tag="VQ497_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HET2517157.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPGWQLQQTSQWMNQAQRLRPHFWVYLQRDEQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            5703..7160
                     /locus_tag="VQ497_000819"
     CDS             5703..7160
                     /locus_tag="VQ497_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2517158.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            7183..7464
                     /locus_tag="VQ497_000820"
     CDS             7183..7464
                     /locus_tag="VQ497_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2517159.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            7541..8026
                     /locus_tag="VQ497_000821"
     CDS             7541..8026
                     /locus_tag="VQ497_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2517160.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            8043..8708
                     /locus_tag="VQ497_000822"
     CDS             8043..8708
                     /locus_tag="VQ497_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HET2517161.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            8712..9575
                     /locus_tag="VQ497_000823"
     CDS             8712..9575
                     /locus_tag="VQ497_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HET2517162.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKEIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SRGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            9577..10281
                     /locus_tag="VQ497_000824"
     CDS             9577..10281
                     /locus_tag="VQ497_000824"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HET2517163.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVAWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            10460..12133
                     /locus_tag="VQ497_000825"
     CDS             10460..12133
                     /locus_tag="VQ497_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET2517164.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            12245..13336
                     /gene="ulaG"
                     /locus_tag="VQ497_000826"
     CDS             12245..13336
                     /gene="ulaG"
                     /locus_tag="VQ497_000826"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HET2517165.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            13450..15426
                     /gene="tkt"
                     /locus_tag="VQ497_000827"
     CDS             13450..15426
                     /gene="tkt"
                     /locus_tag="VQ497_000827"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET2517166.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVTDYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            15543..15842
                     /gene="yajC"
                     /locus_tag="VQ497_000828"
     CDS             15543..15842
                     /gene="yajC"
                     /locus_tag="VQ497_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET2517167.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVEGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            15889..16317
                     /locus_tag="VQ497_000829"
     CDS             15889..16317
                     /locus_tag="VQ497_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HET2517168.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            16319..16729
                     /locus_tag="VQ497_000830"
     CDS             16319..16729
                     /locus_tag="VQ497_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517169.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            17029..19680
                     /gene="adhE"
                     /locus_tag="VQ497_000831"
                     /gene_synonym="adhC"
     CDS             17029..19680
                     /gene="adhE"
                     /locus_tag="VQ497_000831"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HET2517170.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITEPSMEATSALMNHEGIATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
     gene            complement(20665..20973)
                     /locus_tag="VQ497_000832"
     CDS             complement(20665..20973)
                     /locus_tag="VQ497_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2517171.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
     gene            complement(20970..21278)
                     /locus_tag="VQ497_000833"
     CDS             complement(20970..21278)
                     /locus_tag="VQ497_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2517172.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            complement(21283..22578)
                     /locus_tag="VQ497_000834"
     CDS             complement(21283..22578)
                     /locus_tag="VQ497_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2517173.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTKFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPSAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGIVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            complement(22596..24005)
                     /locus_tag="VQ497_000835"
     CDS             complement(22596..24005)
                     /locus_tag="VQ497_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET2517174.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTEDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSLCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            24163..24891
                     /locus_tag="VQ497_000836"
     CDS             24163..24891
                     /locus_tag="VQ497_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2517175.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            25351..25545
                     /locus_tag="VQ497_000837"
     CDS             25351..25545
                     /locus_tag="VQ497_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2517176.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSDVTLITGKNGTGKSTLL"
     gene            complement(25911..27221)
                     /locus_tag="VQ497_000838"
     CDS             complement(25911..27221)
                     /locus_tag="VQ497_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET2517177.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNFAITNGTLDVLAQHILYRTRTYPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTSIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            27458..28330
                     /locus_tag="VQ497_000839"
     CDS             27458..28330
                     /locus_tag="VQ497_000839"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET2517178.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAILLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT         8903 a         5055 c         5921 g         8529 t
ORIGIN      
        1 gtgcattggt tagttgttta ctttcttcat aaattttatt gtatcatatt tttgtttcac
       61 aggaagtcta ataaataaaa attctttaaa attttgttgg gaaaatggga aaagatataa
      121 atggaaactt agtataacac ttggaaaact ctgtaaaaat agtaagtcaa ttcatgtccc
      181 gttttttctc tagggaagtt gggcggctat atttatatga ccatttgtcg taataaacat
      241 ccctaccaag ggtcaataga aataaattca acgaaacttt tgctgtcaga tttagatgcc
      301 caatttgtca tttatactct ttcatatagg actttctata aggatggaaa aaagaaggga
      361 aatttgataa gataggaata tggattttga aaaaattgaa caagcttata cctatttact
      421 agagaatgtc caagtcatcc aaagtgattt ggcgaccaac ttttatgacg ccttggtgga
      481 gcaaaacagc atctatctgg atggtgaaac tgagctaaac caggtcaaag acaacaatca
      541 ggcccttaag cgtttagcac tacgcaaaga agaatggctc aagacctacc agtttctctt
      601 gatgaaggct gggcaaacag aacccttgca ggccaatcac cagtttacac cggatgctat
      661 tgctttgctt ttggtgttta ttgtggaaga gttgtttaaa gaggaggaaa ttactatcct
      721 cgaaatgggt tctgggatgg gaattctagg cgctactttc ttgatctcgc ttgataaaaa
      781 ggtggattac ttgggaatgg aagtggatga tttgctgatt gatctggcag ctagcatggc
      841 agatgtaatt ggtttgcagg ctggctttgt ccaaggagat gccgttcgtc cacaaatgct
      901 caaagaaagc gatgtggtca tcagtgactt gcctgttggc tattatcctg atgatgccgt
      961 tgcgtcgcgc catcaagttg cttctagcca agaacatact tacgcccatc acttgctcat
     1021 ggaacaaggg cttaagtacc tcaagtcaga cggatacgct atttttctag ctccgagtga
     1081 tttgttgacc agtcctcaaa gtgatttgtt aaaagaatgg ctgaaagaag aggcgagtct
     1141 ggttgctatg attagtctgc ctgaaaatct ctttgctaat gctaaacaat ctaagactat
     1201 ttttatccta cagaagaaaa gtgaaatagc agtggagcct tttgtttatc cacttgctag
     1261 cttgcaagat gcaagtgttt taatgaaatt taaagaaaat tttcaaaaat ggactcaagg
     1321 tactgaaata taaaatagat tttgttataa tagttgaaaa cgcttaaaaa ggggtatcat
     1381 gttatgacaa aaacaattgc aatcaatgca ggaagttcaa gtttgaaatg gcaattatac
     1441 ttaatgccag aggaaaaagt gttagccaaa ggtttgattg aacgtatcgg tttgaaagat
     1501 tcaatttcaa ctgtaaaatt tgacggccgt tctgaacaac aaattttgga tatcgaaaat
     1561 catacacaag ctgttaaaat tttattggat gacttgattc gtttcgatat tatcaaggct
     1621 tatgacgaga ttacaggtgt tggccaccgt gttgtagcag gtggagaata tttcaaagaa
     1681 tcaacagttg ttgagggaga tgttttagaa aaagttgaag agttgagttt gttggctcct
     1741 ctacacaacc cggccaatgc agcaggtgtt cgtgctttca aggaattgtt gccagacatt
     1801 accagtgtag ttgtttttga tacttccttc cacacaagta tgccagagaa agcttatcgc
     1861 taccctctac caacaaaata ttacacagaa aacaaggttc gtaaatacgg tgctcatggt
     1921 acaagtcacc agtttgtagc aggagaagct gcaaaactct tgggacgtcc attagaagac
     1981 ttgaagttaa ttacctgtca tattggtaac ggaggctcaa ttacagctgt gaaagccggc
     2041 aaatctgtag acacttctat ggggttcact cctcttggtg gtattatgat gggaacgcgt
     2101 acaggggata ttgatccagc tatcattcct tatttaatgc aatatacaga ggattttaac
     2161 acaccagaag atatcagtcg tgttcttaac cgtgaatcag gtcttttggg agtttctgct
     2221 aattctagcg atatgcgcga tatagaagca gctgtagcag aagggaatca cgaggctagc
     2281 ttggcttatg aaatgtatgt tgaccgtatc caaaaacata tcggtcagta ccttgcagtg
     2341 ctaaatggaa cagatgccat tgttttcaca gcaggtgtcg gtgaaaatgc agagaatttc
     2401 cgtcgtgatg taatctcagg gatttcgtgg tttggttgtg atgttgatga tgaaaagaat
     2461 gtctttggcg ttacaggaga catctcaaca gaggcagcta aaatccgtgt cttggttatt
     2521 ccaacagatg aagaattagt cattgcccgt gacgttgaac gcttgaaaaa ataagtgaaa
     2581 ctaaaaaaat attcaataca aggagttggg aaagttattt ttccagcttc tttttctgat
     2641 gaaattgtcc aaaaccttgc tatgattggc ttttttgaaa aatatggtat aatagtagta
     2701 atttaataga tggagttgag ttttgaagaa aaactttcgt gtaaaaagag agaaagattt
     2761 taaggcgatt ttcaaggagg ggacaagttt tgctaatcgc aaatttgtgg tctaccaatt
     2821 agaaaaccag aaaaaccatt ttcgagtagg tctatcagtt agcaaaaaac tggggaatgc
     2881 cgtcactaga aatcaaatta agcgacggat tcggcatatt atccagaatg caaaagggag
     2941 tctggtagaa gatgtcgact ttgttgtcat tgctcgaaaa ggagtcgaaa ccttgggata
     3001 cgcagagatg gagaaaaatc tactccatgt attaaaatta tcaaagattt accgggaagg
     3061 aaatgggagt gaaaaagaaa ctaaagttga ctagtttgct aggactgtct ctgttaatca
     3121 tgacagcctg tgcgactaat ggggtaacta gcgatattac agccgaatcg gctgattttt
     3181 ggagtaaatt ggtttacttc tttgcgaaaa tcattcgctt tttatcgttt gatattagta
     3241 tcggagtggg gattattctc tttacggtct tgattcgtac agtcctcttg ccagtctttc
     3301 aggtgcaaat ggtggcttct aggaaaatgc aggaagctca gccacgcatt aaggcgcttc
     3361 gagaacaata tccaggtcga gatatggaaa gcagaaccaa actagagcag gaaatgcgta
     3421 aagtatttaa agaaatgggt gtcagacagt cagattctct ttggccgatt ttgattcaga
     3481 tgccggttat tttggccttg ttccaagccc tatcaagagt tgacttttta aagacaggtc
     3541 atttcttatg gattaacctt ggtagtgtgg atacaaccct tgttcttccg attttagcag
     3601 cagtattcac ctttttaagt acttggttgt ccaacaaagc tttgtctgag cgaaatggcg
     3661 ctacgactgc gatgatgtat gggattccag tcttgatttt tatctttgca gtttatgcgc
     3721 caggtggagt cgccctatac tggacagtgt ctaatgctta tcaagtcttg caaacctatt
     3781 tcttgaataa tccattcaag attatcgcag agcgcgaggc cgtagtacag gcacaaaaag
     3841 atttggaaaa tagaaaaaga aaagccaaga aaaaggctca gaaaacgaaa taaataagga
     3901 ggaatctggt aatggtagta tttacaggtt caactgttga agaagcaatc cagaaaggat
     3961 tgaaagaatt agatattcca agaatgaagg ctcatatcaa agtcatttct agggagaaaa
     4021 aaggctttct tggtctattt ggtaaaaaac cagcccaagt ggatattgaa gcgattagtg
     4081 aaacgactgt tgtcaaagca aatcaacagg tagtaaaagg cgttccgaaa aaaatcaatg
     4141 atttgaacga gcctgtgaag acggttagtg aagaaaccgt tgaccttggt catgtggttg
     4201 atgctattaa aaaaatagag gaagaaggtc aaggtatttc tgatgaagtc aaggctgaaa
     4261 tcttaaaaca tgaaagacat gccagcacta tcttagaaga aactggtcac attgagattt
     4321 taaatgaact tcaaatcgag gaagcgatga gggaagaagc aggcgctgat gaccttgaaa
     4381 ctgagcaaga tcaaactgaa aatcaagact tgaaagagat gggcttgaag gtcgagcaaa
     4441 gttatgatat tgcccaggtg gctacggatg tgactgccta tgttcaagcg attgtggatg
     4501 acatggatgt tgaagctaca ctttcaaatg attataaccg tcgtagcatc aatctacaaa
     4561 ttgacaccaa cgaaccaggt cgtattatcg gctaccatgg taaagtcttg aaggccttgc
     4621 aactgttggc tcaaaattat ctttacaacc gctattccaa aaccttctac gttacaatca
     4681 atgtcaatga ttatgtcgaa caccgtgcag aagtcttgca gacctatgcg caaaaattgg
     4741 cgattcgtgt tttagaagaa ggacgtagtc atcaaacaga tccaatgtcg aatagtgaac
     4801 gcaagattat ccaccgtatt atttcacgta tggatggcgt gaccagttac tctgaaggtg
     4861 atgagccaaa tcgctatgtc gttgtagata cagaataagt aaaatcaggg ttgacctgat
     4921 tttttgctag ctagaggagg ttaaactaat gttgaataag ataagagact atttagactt
     4981 tgctggtttg cagtaccgta atcctgataa agcgggagca gagcgagaga agatgctggc
     5041 attccgccac aaaggacaag aggcccgaaa ggcttttaca gaactggcca aagcctttca
     5101 agcaagccat ccaggatggc aactccaaca gactagtcag tggatgaatc aggcccagcg
     5161 tttgagacca catttttggg tttatctaca gagagacgaa caagtgacag aacctatgat
     5221 ggccttacgt ttgtatggga catctactga ctttggaatt tctttggaag tcagtttcat
     5281 cgaacgtaag aaggatgagc aaacactggg caagcaggcc aaagttttag acattccaac
     5341 cgttaaaggg atttattatc taacctactc taatggtcaa agtcaacggt gggaggcgaa
     5401 tgaagaaaag cgtcgtactt tacgcgagaa ggtgagaagt caagaagttc gaaaagtttt
     5461 agtgaaggta gatgttccta tgacagaaaa ttcgtctgaa gaagaaatcg tagaaggctt
     5521 attgaagtct tattctaaaa ttcttcccta ttatctagct acgagaaaat aagataattt
     5581 gtaaaacatc ataaatcata cagtccaaga gtgaacagtc cgctgtgtaa ttcttggtct
     5641 ttttgtttgc gctttcgcat tatataataa acttacaaaa acaattcaaa aggagaacaa
     5701 ttatggaagt cgtttcaagt gttctaaatt ggttttctag caatattttg cagaatcccg
     5761 catttttcgt aggtttattg gtgttgatag gatatgcact tttgaaaaaa cctgcccatg
     5821 acgttttttc agggtttgtt aaagcaacag tagggtatat gttgcttaac gtgggtgctg
     5881 gtggtttggt tacaaccttt cgtccaatct tagcagctct taactacaaa ttccaaattg
     5941 gtgcagcggt tatcgaccct tactttggac ttgctgcagc aaacaacaaa attgcagcag
     6001 agtttccaga ttttgttgga actgcaacta cagctctatt gattggtttt ggaataaata
     6061 tcttgctcgt agctcttcga aagattacga aggtaagaac cctctttatt actggtcaca
     6121 tcatggtaca acaagctgca acagtatctc ttatggttct attcttagta ccacaattgc
     6181 gcaatgctta cggtacagca gcgattggta tcatctgtgg actttactgg gcagttagtt
     6241 caaatatgac tgttgaggca actcaacgct tgactggtgg tggcggattt gcgattggtc
     6301 accaacagca atttgcaatc tggtttgtag ataaagtagc aggacgcttt ggtaagaaag
     6361 aagaaagttt agacaatctt aaattaccta agttcctctc aatcttccac gatacagttg
     6421 ttgcatctgc taccttgatg ctcgtattct tcggagccat tcttttaatc ttgggtccag
     6481 acattatgtc taataaagaa gtcatcactt caggaactct attcaatcct gctaaacaag
     6541 atttctttat gtacattatc caaacagcct ttaccttctc agtttacttg ttcgttttga
     6601 tgcaaggtgt ccgaatgttc gtatctgagt tgacaaacgc cttccaaggt atttcaaaca
     6661 aattgttgcc aggttcattc ccagcggttg acgttgcagc ttcttatgga tttggttctc
     6721 caaatgctgt cttgtcagga tttacctttg gtttgattgg tcaattgatt acaattgttt
     6781 tgctcatcgt ctttaaaaat ccgattctta ttattacagg atttgtacca gtgttctttg
     6841 acaatgcagc cattgcggtc tacgctgata aacgcggcgg atggaaagcg gctgttatcc
     6901 tttcctttat atcaggtgtc cttcaagttg ctctaggagc tctttgtgtg gcccttctcg
     6961 atttggcatc ttatggtggc taccatggaa atatcgactt tgaattccca tggcttggat
     7021 ttggatatat cttcaaatac cttggtattg ttggttatgt acttgtgtgt ctcttcttgc
     7081 ttgttattcc tcaacttcaa tttgccaaag caaaagataa agagaaatat tacaacggtg
     7141 aagttcaaga agaagcttag tatctagaaa aggagaaata aaatggttaa agtattagca
     7201 gcgtgcggaa atggaatggg ttcatcaatg gttatcaaga tgaaggttga aaatgctctc
     7261 cgtaagctta atcaaacaga ttttacagtc aattcatgca gtgtcggtga agctaaaggt
     7321 ttagcagtag gatatgacat tgtaatcgct tctcttcatt tgattcaaga attggaaggg
     7381 cgaactaatg ggaagttaat tgggcttgat aacttgatgg atgataaaga aatcaccgaa
     7441 aaactcagtc aagcactaca gtaaaaggtt ggagggggct ggacagaaac tgagagttat
     7501 cgtttctgtc cagccccctc tttaaataaa ggaggcagat atgaatttaa aacaagcttt
     7561 aattgacaat gactcgatcc gactaggttt agaggctaac aattggaaag aagcagtcaa
     7621 ggtagcagta gatcccttga ttgaaagtgg ggcaattttg ccagagtatt acgatgctat
     7681 cattgaatcg actgaagagt atgggcctta ctatatcttg atgccaggta tggctatgcc
     7741 ccacgctaga cctgaagctg gtgtgcaaag tgatgccttt tcattgatta ccttacaaaa
     7801 tcctgttgta ttttcagatg ggaaagaggt atctgttttg ttggcactag cagcaacaag
     7861 ttcaaaaatt cacacaagtg tagccattcc acaaattatt gccctgtttg aattagaaga
     7921 ttctattgca cgtttacagg cttgccagac taaagaagat gtcttggcta tgattgaaga
     7981 atctaaggat agcccttatc tcgaaggatt ggatttggaa agttagaaag aggaataaag
     8041 aaatgacaaa aagaatacct aatttacaag ttgcattaga ccattcagac ttgcaaggag
     8101 cgattaaagc agctgtttct gttggtcagg aagtagatat tatcgaagct ggaactgttt
     8161 gcttgcttca agttggaagt gaactggctg aagtcttgcg tagccttttc ccagataaga
     8221 ttattgtggc agacacaaaa tgtgctgatg ctggtggaac agttgctaaa aataatgcgg
     8281 ttcgtggagc agactggatg acttgtatct gttgtgcaac catccctact atggaagcag
     8341 ctctaaaggc tatcaagact gaacgaggag aacgaggcga aatccagatc gagctttatg
     8401 gcgattggac ttttgaacaa gctcagcttt ggctagatgc aggtatctca caagctattt
     8461 atcaccaatc tcgtgatgct cttcttgctg gtgaaacttg gggtgaaaaa gaccttaata
     8521 aggttaaaaa actcattgac atgggcttcc gtgtatctgt aacaggtggt ctagatgtag
     8581 atactctcaa actctttgaa ggtgttgatg tctttacctt tatcgcaggt cgtggaatta
     8641 cagaggctgc ggatccagca ggagcagcgc gtgccttcaa ggatgaaatc aaacgaattt
     8701 gggggtaaat catggtacgt ccaattggaa tttatgaaaa ggcaacccca acacacttta
     8761 cttggctaga acgtttaaat tttgccaagg agttaggctt tgattttgtc gagatgtcta
     8821 ttgacgaacg tgacgagcgt ttagcaagac ttgactggag taaggaagaa cgcttggaag
     8881 ttgtcaaaga aatctatgaa actggtgttc gtattccttc tatctgtttt tcaggccatc
     8941 gtcgctaccc attgggttca aaagatccag ttctagagga aaaatctcta gaactcatga
     9001 aaaaatgtat cgaattagct caagacttgg gagttcgtac gattcaatta gctggttacg
     9061 atgtttacta tgaggaaaag tcaccccaga cacgccaacg ttttatcaaa aatttgagaa
     9121 aagcctgtga ctgggctgaa gaagctcagg tggtacttgc tattgaaatt atggatgatc
     9181 ctttcatcaa tagcatcgaa aaatatttgg ctatagaaaa agagattgac tctcccttcc
     9241 tctttgtata tccagatatt ggtaatgtgt ctgcatggca taatgatatc tatagtgagt
     9301 tttatcttgg tcatcatgcc atcgcagctc tccatctcaa ggatacttat gcagtgacag
     9361 aaagttcaag gggccagttc cgagatgtac cttttgggca aggttgtgtc aaatgggaag
     9421 aagctttcga tattttaaag gaaaccaatt ataatggacc tttcctaatc gaaatgtggt
     9481 ctgaaaattg tgaaacagta gaagaaacac gcgcagccat tcaagaggcg caagcttttc
     9541 tctatccact cattaagaaa gcaggtttga tgtaagatga atcaagtaat caatgctatg
     9601 cgtaaacgag tctgtgatgc caatcaatca ttgccaaaac atggacttgt caaatttacc
     9661 tgggggaatg tatctgaagt caatcgcgaa ctcggtgtca ttgttatcaa accatcaggc
     9721 gtggattatg acgaattgac acctgaaaac atggtagtga ctgatctaga tggtaagatc
     9781 ctagaagggg atttaagacc atcttccgac ctcccaactc atgtgcaatt atataaggct
     9841 tggtcagaaa ttggtagtgt ggttcacacc cattcgacag aagctgttgc ttgggctcag
     9901 gcaggtcgtg atattccttt ctacggaaca acccatgcag attatttcta cggttcaatc
     9961 ccttgcgccc gtagtttgac caaggacgaa gtagaagtgg cctatgaaaa agatactggc
    10021 ctggttatcg tagaagagtt tgaacatcgc ggacttaacc cggttgaagt accaggaatt
    10081 gttgtacgca atcacggtcc attcacctgg ggcaaaaatc cagagaatgc tgtttatcac
    10141 tctgtcgtac tagaggaagt atcaaagatg aatcgcttta cagaacaaat caatccaaga
    10201 gttggacctg ctccccagta catactagaa aaacactacc aacgtaaaca tggaccaaat
    10261 gcttattatg gtcaaaagta agaacgatga aggaggagaa aaagataaat ttagctcctc
    10321 tttttacatt tgatttttat tgagagtaaa gttggagttg aagtaatttt aaaagatttt
    10381 ttagaaatag cgcttgatat atatatggta aaataaaaag aattgctgtg atatcaatag
    10441 atttggggga ttttttaata tggtactgga taaggcaagt tgtgatttgc ttcaatattt
    10501 gatggatcaa gaaacgtcca aaacgattat ggcgatttcg aaagatttga aagagtcaag
    10561 aaggaaaatt tattatcaca ttgacaaaat caatgctgct ctgggtgacg aggcgcttca
    10621 catcattagt attccacgaa ttggtattca cttaacggaa gagcagagag atgcttgttg
    10681 taaactatta tcggaagtag attcgtacga ttatatcatg agtgcgcatg aacgtatgat
    10741 gataatgtta ctatggatag gtatttctaa agaacgtatt acgattgaaa aattgataga
    10801 gttaacagag gtatctagga atactgttct caatgatttg aatagtattc gttatcaact
    10861 aactttggaa caatatcagg tgatcttgca agtgagcaag tcacagggat accaccttca
    10921 tgcccaccct cttaataaaa ttcagtatct tcaatcgctt ctatatcata tttttatgga
    10981 agaaaatgcc acttttgtat ctattttaga agataagatg aaagagaggt tagatgatga
    11041 gtgtttgctt tctgttgaaa tgaaccaatt ttttaaggaa caggttcctt tagttgaaca
    11101 agatttaggg aagaaaataa accatcatga aataactttt atgttgcagg ttctacctta
    11161 tttgctgtta agctgtcata atgttgaaca gtatcaagaa agacatcagg atatagagaa
    11221 agaattttct ttgataagaa aaagaataga gtatcaggtg tctaagaaat taggagaacg
    11281 gttgtttcaa aagtttgaaa tttctttgtc aggacttgaa gtttctcttg tagctgttct
    11341 cctcctctcc tatcgtaaag atttggatat tcatgcagaa agtgatgatt ttcggcaatt
    11401 aaaacttgct ttagaagaat ttatctggta ttttgaatca caaatccgaa tggagattga
    11461 gaacaaggat gatttgttac gaaatttgat gatccactgt aaagccttgt tatttagaaa
    11521 gacttacggt attttttcta aaaatcctct aacaaaacaa attcgatcca agtatggaga
    11581 attattttta gtcactagaa aatctgcgga aattttagaa ggagcatggt ttattcggct
    11641 aacagacgat gatattgcct atttgacgat tcatattgga ggatttttaa aatatacacc
    11701 atcatctcaa aaaaatatga aaaaagttta tctcgtttgt gatgaaggtg ttgcggtttc
    11761 gagacttttg ctgaaacaat gcaaacttta ttttccaaat gagcaaattg acactgtatt
    11821 tacaacagaa caatttaaga gtgtggaaga tattgcacaa gttgatgtag tgattactac
    11881 taatgatgat ttggatagca gatttccgat tttaagggtt aatcctatcc ttgaagcaga
    11941 agatattttg aaaatgctag actatcttaa acacaatata tttcgtaata agagcaaaag
    12001 tttcagtgaa aatctttcta gtcttatttc gtcttatatt gtagacagca agttggctag
    12061 taagttccaa gaagaggttc aaacacttat aaatcaagaa atagtagttc aagctttttt
    12121 ggaagatatt tgaaggacag tccaatgatg aacacaaacc tgtgtttttc ttggtctttt
    12181 ttagtgtttt gaagggtggt atactaatct caaagataac aattatatcc aaaggaggca
    12241 acatatgcca aacgtcaaag aaattacaag agagtcatgg attttagcca ctttcccaga
    12301 gtggggaaca tggttgaacg aagaaatcga agaagaagtc gtacctgaag gcaactttgc
    12361 catgtggtgg ctaggcaact gtggtacttg gattaagaca ccagctggtg ctaacgttgt
    12421 catggacctt tggtcaaacc gtggaaaatc aaccaaaaaa gtgaaagata tggttcgtgg
    12481 gcaccaaatg gcaaatatgg caggtgttcg taagctgcaa ccaaacttgc gtgttcagcc
    12541 aatggttatc gatccatttg ctatcaacga actagactat tacttagttt cacacttcca
    12601 cagtgatcat atcgacccat acacagctgc agcaattctc aataatccta agttagagca
    12661 tgttaagttt atcggtcctt accactgtgg acgaatctgg gaaggatggg gtgttccaaa
    12721 agaacgtatc atcgttgtta aaccaggtga cactatcgaa ttaaaagata tgaagattca
    12781 tgcagtagaa tcatttgacc gtacttgctt ggtaactctc ccagtgaacg gtgctgatga
    12841 gacaggcggt gaacttgctg gcttggctgt tacagatgaa gaaatggctc aaaaggctgt
    12901 taactatatc tttgaaacac caggtggaac catctatcat ggtgcagatt ctcacttctc
    12961 aaactatttt gcaaaacatg gtaaagactt taaaattgat gttgctttga ataactatgg
    13021 tgaaaatccg gtaggtatcc aagacaaaat gacatctatc gaccttcttc gtatggcaga
    13081 aaatctgcgt accaaagtca ttatcccagt tcactatgat atctggtcta acttcatggc
    13141 ttctactaat gagattctag aactttggaa aatgcgaaaa gatcgcttgc aatacgattt
    13201 ccatccattt atctgggaag ttggcggtaa gtacacttat cctcaagatc aacacttagt
    13261 agaataccat catccacgtg gttttgatga ttgttttgaa caagactcta acattcaatt
    13321 taaagctttg ctataatata aaaatattct ttggaggtta tccgtatgtt gcatgatacg
    13381 gataattttc atataatagt aaaatagaat gtgtgattca ataatcacct caaatagaaa
    13441 ggaaattcta tgtcaaatct atctgttaat gcaattcgtt ttctaggtat tgacgccatt
    13501 aataaagcca actcaggtca tccaggtgtg gttatgggag cggctccgat ggcttacagc
    13561 ctctttacaa aacaacttca tatcaatcca gctcaaccaa actggattaa ccgcgaccgc
    13621 tttattcttt cagcaggtca tggttcaatg ctcctttatg ctcttcttca cctttctggt
    13681 tttgaagatg tcagcatgga tgagattaag agtttccgtc aatggggttc aaaaacacca
    13741 ggtcacccag aatttggtca tacggcaggg attgatgcta cgacaggtcc tctagggcaa
    13801 gggatttcaa ctgctactgg ttttgcccaa gcagaacgtt tcttggcagc caaatataac
    13861 cgtgaaggtt acaatatctt tgaccactat acttacgtta tctgtggaga cggagacttg
    13921 atggaaggtg tctcaagcga ggcagcttca tacgcaggct tgcaaaaact tgataagttg
    13981 gttgttcttt atgattcaaa tgatatcaac ttggatggtg agacaaagga ttcctttaca
    14041 gaaagtgttc gtgaccgtta caatgcctac ggttggcata ctgccttggt tgaaaatgga
    14101 acagacttgg aagccatcca tgctgctatc gaaacagcaa aagcttcagg caagccatct
    14161 ttgattgaag tgaagacggt tattggatac ggttctccaa acaaacaagg aactaatgct
    14221 gtacacggcg cccctcttgg agcagatgaa actgcatcaa ctcgtcaagc cctcggttgg
    14281 gactacgaac catttgaaat tccagaacaa gtatatgctg atttcaaaga acatgttgca
    14341 gaccgtggcg catcagctta tcaagcttgg actaaattag ttacagatta taaagaagct
    14401 catccagaac tggctgcaga agtagaagcc atcatcgacg gacgtgatcc agtcgaagtg
    14461 actccagcag acttcccagc tttagaaaat ggtttttctc aagcaactcg taattcaagt
    14521 caagatgcct tgaatgttgt ggcagctaaa ttaccaacct ttctaggtgg atcagctgac
    14581 cttgctcact caaacatgac ttatatcaag acggatggac ttcaagacga tgccaatcgc
    14641 ttgaatcgta acattcagtt tggtgttcgt gaatttgcaa tgggaacaat cttgaacggg
    14701 atggcccttc atggtggact tcgtgtatac ggtggaactt tcttcgtctt ctctgactat
    14761 gtgaaggcag ctgtccgctt gtcagcctta caaggacttc ctgtgactta tgtctttacc
    14821 catgattcaa tcgcagttgg ggaagatggt ccgactcatg aaccagttga gcatttagca
    14881 ggtcttcgtg ctatgccaaa tctaaatgtt ttccgtccag cagatgcgcg tgaaacgcaa
    14941 gcagcttggt accttgcagt gacaagtgag aaaacaccaa ctgcccttgt cttgacacgt
    15001 caaaatttga ctgttgaaga tggaacagac ttcgacaagg ttgctaaagg tgcttatgtt
    15061 gtatatgaaa atgcagccga ctttgatacc atcttgattg cgacaggttc agaggttaat
    15121 cttgctgtct cagctgctaa agaattggct agtcaaggcg aaaaaatccg cgtagtcagc
    15181 atgccatcta cagatgtctt tgataaacaa gatgcagctt acaaggaaga aattcttcca
    15241 aatgcagtcc gccgtcgtgt tgcagtcgaa atgggtgcaa gtcaaaactg gtacaaatat
    15301 gttggtctcg atggtgccgt tctaggtatt gataccttcg gagcctctgc cccagcacca
    15361 aaagtattgg cagaatatgg ctttactgta gaaaatcttg taaaagttgt tcgaaacttg
    15421 aaataatcct aaaaatcagg gcgtaagctc tggtttttct taccagaaaa gtaaggtaca
    15481 atcttgtaaa agtagctgaa atttgatata gtagtcctat gtaaaagaca aaggagaaaa
    15541 caatgaatcc aaatattact tttttaatca tgcttgtagg tatgatggcc ttgatgttct
    15601 ttatgcaacg ttctcaaaag aaacaagctc aaaagcgtat ggaaagctta aataaactac
    15661 aaaaaggcta tgaagtgatt acaatcggtg gactttatgg aacagtcgat gaagtagata
    15721 cggagaaggg aacaatagtt cttgacgtag agggagttta cttgactttt gaactagctg
    15781 ctatcaagac agtattaccg ctgaaagaaa cagcttcact agaaggcgca attgaaaaat
    15841 aagacgggat cacgaactcc cgtttttcta taaaagaaag gaaatgggat gaaaaaatta
    15901 gtctttgtct gtctgggaaa tatttgccgt agccctatgg ccgagtttgt tatgaaatca
    15961 atgacagata actacgaaat ccaaagtcga gcaacttcct cttgggaaca tggcaatccg
    16021 attcataagg ggactcaggg aatttttcaa gagtatgaga ttccttatga caagaacaag
    16081 acatcgcttc agattagtaa ggaagatttt gaagcctttg attatattat cggaatggac
    16141 gcttcaaata tttctgactt acgtcagatg tgtccagtag actgtcaaga taagatttac
    16201 tcattttcat ctgaaagtgt tccagaccct tggtatacag gagattttga agaaacctat
    16261 cgacgtgttc aagagggctg tcaagcttgg ttagaacgtt tagaaaagga gagttaaggt
    16321 ggaaaatctt acgaattttt acgaaaagta tcgtgtctat ctgactcgtc cacgtttaga
    16381 gcttttggca gtagttacca ttgttttctg tgctgtactc gtcttttttc taaatattcc
    16441 aggaaaaggt gtcttaaaac tcgataatgg aacgattgtt tatgatggca gtcttgtccg
    16501 cggtaaaatg aatggccaag gtaccattac cttccaaaat ggagaccaat atacaggtgg
    16561 cttcaacaat ggagccttca acggaaaagg tacctttcaa tctaaagaag gctggaccta
    16621 cgaaggtgat tttgtaaatg gtcaggctga aggaaaaggg aaactaacaa cagaacaaga
    16681 agtcgtttat gaaggaactt ttaaacaagg cgtttttcaa caaaaataaa gcctccttat
    16741 caaaggaggt attattagaa tcgcaaggta agcgcttacc tgcaaatccc tttctttcca
    16801 aatccctctt ccaagcaagt ttgtgaaata aaaaatattt gaaataaatt tcacaaactt
    16861 caaagataaa acctgataag aaaagaaaat gagaaaagtt tcacaagagt ttaaaaattc
    16921 tttgtgaaat gtttatgaaa ctgtttacaa agttgaaata atgcgttata ataaacttgt
    16981 gaaaaaatta acaaaggata tcgttccttg aaagctatgg aggaaaatat ggctgataaa
    17041 aaaactgtga caccagagga aaagaaactc gttgctgaaa aacacgtaga tgagttggtt
    17101 caaaaagctc tagttgccct tgaagaaatg cgtaaattgg atcaagaaca agttgactac
    17161 atcgttgcca aagcatcagt agcagctttg gatgcccacg gagaattggc tttacatgcc
    17221 tttgaagaaa caggacgtgg tgtatttgaa gataaagcaa ctaagaactt gtttgcttgt
    17281 gaacacgtgg taaacaacat gcgtcacact aaaacagttg gcgttatcga agaagatgat
    17341 gtaacaggat tgaccctgat tgctgaacca gttggtgttg tttgtggtat cactccaaca
    17401 acaaacccaa catcaacagc gatcttcaaa tcattgattt cattgaagac acgtaatcca
    17461 atcgtctttg ccttccatcc atcagctcaa gaatcatcag ctcatgcagc tcgtatcgtc
    17521 cgcgatgcag ctatcgcagc tggtgctcct gaaaactgtg tacagtggat cactgaacca
    17581 tctatggaag caactagtgc ccttatgaac cacgaaggta ttgcaacaat ccttgcaaca
    17641 ggtggtaatg ccatggttaa agccgcatac tcatgtggta aaccagctct tggggtaggt
    17701 gccggaaacg ttccagctta tgttgaaaaa tcagcaaaca ttcgtcaagc agcacacgat
    17761 atcgtcatgt ctaaatcatt tgataacggt atggtctgtg catctgaaca agcagttatc
    17821 attgataaag aaatttacga tgaatttgta gcagagttca aatcttacca cacttacttt
    17881 gtaaacaaaa aagaaaaagc tcttcttgaa gagttctgct tcggcgtcaa agcaaacagc
    17941 aaaaactgtg ctggtgcaaa attgaacgct gacatcgttg gtaaaccagc aacttggatt
    18001 gcagaacaag caggatttac agttccagaa ggaacaaaca ttcttgctgc agaatgtaaa
    18061 gaagttggcg aaaatgagcc attgactcgt gaaaaattgt caccagttat tgcagttttg
    18121 aaatctgaaa gccgtgaaga tggtattact aaggctcgtc aaatggttga atttaacggt
    18181 cttggacact cagcagctat ccacacagct gacgaagaat tgactaaaga atttggtaaa
    18241 gctgttaaag ctattcgtgt tatctgtaac tcaccttcta cttttggtgg tatcggggac
    18301 gtttacaatg ccttcttgcc atcattgaca cttggatgtg gttcttacgg acgcaactca
    18361 gttggggata acgttagtgc cattaacctc ttgaatatca aaaaagtcgg aagacggaga
    18421 aataacatgc aatggatgaa acttccttca aaaacatact ttgaacgtga ttcaattcaa
    18481 taccttcaaa aatgtcgtga cgttgaacgt gtcatgatcg ttactgacca tgccatggta
    18541 gagcttggtt tccttgatcg tatcatcgaa caactggacc ttcgtcgcaa taaggttgtt
    18601 taccaaatct ttgcggatgt agaaccggat ccagatatca caactgtaaa ccgtggtact
    18661 gagattatgc gtgccttcaa accagatacc atcatcgcac tcggtggtgg gtctccaatg
    18721 gatgctgcca aagtaatgtg gctcttctac gagcaaccag aagtggactt ccgtgacctt
    18781 gtccaaaaat tcatggatat ccgtaaacgt gccttcaagt tcccattgct tggtaagaag
    18841 actaaattca tcgcgattcc aactacatct ggtacaggat ctgaagtaac accatttgcc
    18901 gttatctctg ataaagcaaa caaccgtaaa tacccaatcg ctgactactc attgacacca
    18961 actgtggcaa tcgtagatcc tgctttggta ttgacagttc caggatttgt tgctgctgat
    19021 actggtatgg acgtattgac tcacgcgaca gaagcatacg tatcacaaat ggctagtgac
    19081 tacactgatg gtttagcact tcaagccatt aaattggtct ttgaaaatct cgaaagctca
    19141 gttaagaatg cagacttcca ctcacgtgag aaaatgcata acgcttcaac aatcgctggt
    19201 atggcctttg ccaatgcctt cctaggtatt tctcactcaa tggcccataa gattggtgcg
    19261 caattccaca caatccacgg tcgtacaaat gctatcttgc ttccatacgt tatccgttac
    19321 aacggtacac gtccagctaa gacagcaaca tggcctaagt acaactacta ccgtgcagat
    19381 gaaaaatacc aagatatcgc acgcatgctt ggacttccag cttctactcc agaagaaggg
    19441 gttgaatctt acgcaaaagc tgtctacgaa ctcggtgaac gtattgggat ccaaatgaat
    19501 tttagagacc aaggaattga cgaaaaagaa tggaaagaac attctcgtga attagccttc
    19561 ctggcttatg aagaccaatg ttcaccagct aacccacgtc ttccaatggt agaccatatg
    19621 caagaaatca tcgaagatgc atactatggc tacaaagaaa gaccaggacg ccgtaaataa
    19681 ttgtttatca gtctagaagc aagacaaaaa ctcaatttga gggaaagatc cagtaatttt
    19741 tctatgataa aaggcatcct atcaaggttt ttgaacacct gataggatgc ctttttatga
    19801 tattgaggcc tttttgccct ttttgaaaaa ctagaataga aacaaaatat ataatagatt
    19861 gaaactggaa tagtacactt ctgcttctaa aacattgtta gaattcgatt tgactgtcct
    19921 gatcgatttg tcctattcct atttcatttt actatataaa aaatatagta tagtagactg
    19981 aatctaaaat agtacgaaac aattgctaaa acatttatag aaattaattt tactttcctg
    20041 atagagttgt tcacatctta tttcaattca ctatagttta atttaagagt agtatttact
    20101 aaggcccaat taaaatcaaa gagcaaacta gaaaacgagt gccattcagc tcaaaacact
    20161 gatttgagat tgcagataag actagcccct cattaacagg tttacgataa aacgatgaca
    20221 aggtgtgttg ctttttgatt tctaaagagt ataatgatag atctctataa aataagtgcg
    20281 aaggaaatga gcttttatag tcctttcgtt ttaaaatact atctcagatg ttcttatatc
    20341 gacaagaagt ttttgagtca ttccctcatc atacatatta aataaatagt ggctcattca
    20401 attttttact agaataataa gctagtatag taaactgaaa taagatataa acaaataaat
    20461 tggagcttaa catctatttc cagcaatttt ttagaaactg cagtggacta ttctaaattc
    20521 aacacattat aaaaactaga gtaaaagaaa aggattggat cttgtgtaat gcaggatcta
    20581 atcctttcaa tcattttgtc caacttttgg aggttcctac aatgtagccg tcattaataa
    20641 agacagatgg gaacgacagt gttcctattt attttgatag agatcgatga attctttaga
    20701 tagcaactga ataatctctg ttgaagccat ttggtcttct gcatgcataa atagcaagga
    20761 gaatcctatt ttttctccag tagcttcttt ttgtatgaga ttagagtgaa tcttgtgcgc
    20821 ttctactaag gagtcttccg cttcttcaac tttaattttc gcttctttta aatttcctgc
    20881 cttagctagt tggatggctt caataaagga tgatttggct gctccactat tggcaatgag
    20941 ctgaaaacag atatattcca tttcttctgt catcttattt ctcctatcca tgcaagtgct
    21001 tgttccagaa cttttgctcc attcatcatt ccgtaatccc gcatatcaat ggtatctaca
    21061 gggatatttc ctgcaatttc tttcacagca agtaactcat aacgaatttg tggcccaatt
    21121 agaatgacat ctgcttcatg gatattcttt ttcgcttctg tcattgattt tgcttggata
    21181 gaaatttcaa tcccacgttc agtcgcactt tgttgcattt ttttaacaag catacttgtc
    21241 gacattcccg cattacatac taataaaatt tgtttcataa tcttaacctt ccatttcttg
    21301 ctcaacaact ttgtcattaa ctttgataaa tggaatgtat agaagaactc caagtgcaaa
    21361 gatgatgaat tgaactagaa ctgctctcac gtcccctgct gttgctaacc atgcatttaa
    21421 gaatactggt gtagtccaag gaacttgtat aaatgcagga ctcatgaatc ctgtaactgt
    21481 tgctaagtag ctgattaaaa taccaaggac tggaactgtg ataaatggaa tagctaatga
    21541 aatgttataa acgattgggt aaccgaataa tactggttca ttgatattga agataccagg
    21601 tccaaaagat aatttagcca cgtttttaga aacagcattg cgactcacta agaatgttgc
    21661 tattaataaa cataatgtag atccactacc acccattaaa gcgaatgttt gtatttgtga
    21721 taagttgatg atgtgtggaa tggcttgtcc attatttgct gcagtgatgt tttcagtaat
    21781 gttaattaat agtaatggtt ctaggatggc actgtaaata actgcttggt gaataccaaa
    21841 tagccataac atatttccta aagagtaaat aataatgacc ccgattaagc ttgtaccaat
    21901 atgacgaatt ggttcttgaa taaagattgt aatgattgag attaagttca ttccagttat
    21961 attgaataat aatgctgaaa caatcccaaa taaggagatg acagtcatga ctggaagtaa
    22021 tacgctaaat gatctactaa cagctggtgg aatattttca ccaaggttca tttgtaaagc
    22081 tttaacgttt gataattcaa tgaataattc tgttgcaata atcgctacga taaccccggc
    22141 gaacattgcg cctgtacctg tgttgttgaa tgaaagaaca cctgaaatgt ttacagcatc
    22201 ttttgctccg tcaggaacta cagaaactgt atttggcatc atcacaatta aagaaactaa
    22261 tgatagcatt gatgctgctg acgggttttc gaaatctctg tttttagcta agaaataacc
    22321 aaccattaca gcaataatca tacctgaaat acttaaagta ccgtttgcaa ttgttattcc
    22381 ccaatattgg aattttgtta atgtatcccc ttggaaaatc cacttaaata ccgtgttgtt
    22441 caaaagaacg attaaacctg ccaaaatata taatggcatt actgttacga atgcatctct
    22501 tagggttttt aaatgaattt ggttccctag tttaccagca aaggtggcaa attgatctat
    22561 aaattttgag tttttcatct gaatatctcc tctctttact cctcaatttc tacttcaaaa
    22621 ccgttattgt cacttacttt gcgataccat cttcctgagc tcttgatggt tctcttttgc
    22681 gtttctaaat caacggagat aaatccgtaa cgattcttat atgcattatt ccaagaccag
    22741 caatcaaatg cggtccaagc gtggtatcca aaacagagac ttccctcttc aatggcttta
    22801 tgtagccatc ttaaatgttc ttcataaaat tcaatacgat acacgtcatc gataactcca
    22861 ttttcatcga taaaccgtgc ttcgttttca acacccattc cgttctcact gataaaccat
    22921 gggatattac catattcttc cttcacaatc atagcaatat cataaatggc tttcggaaaa
    22981 atttcccaac cacgatatgg attcatgcga cgctccggcc attcatactc tttgaagtat
    23041 tggtctggca tccagggcgt ctgatattcc tcaggatttg cttgtgcttg aacacgtttt
    23101 ggatggtagt agtttactcc aagaaaatca accgtatttg atttcatcag ttgaagctct
    23161 ttttcggtat gactccataa cacgccatct ctctctagct gttttaccaa tctttctggg
    23221 aaagttcctt taacagctgg attcaagaag actttgttaa agaagtcttc tgtaaatcga
    23281 cttgcttcta agtcttctgg agaattactt cttggataag caggtgtcaa gtttaaaata
    23341 atcccaatct ttccatcaag tcctaatgag cgatatagtt gaatcacttt tgcactagca
    23401 aggtttagat tatagatgac ttgtacggcc tcttttccct ttccttttag atttggataa
    23461 tggaaagcat ataagtatcc tgcttctgga atgaccattg gctcattgaa agttgtccag
    23521 taatgaacct tatctccaaa gcatgtgaaa gcagtcttgg caaacttcac gaataactcc
    23581 actacatgtt tgctttccca accaccgtat ttttgaagaa gttccactgg taaatcaaaa
    23641 tgatgtaaat tcatcacaag atccatctgg ttctttttag cttcttcaat gatggcattg
    23701 tagaaagcaa taccttttgg atcaggctca cctgtctcta aattcttgat gagtcgactc
    23761 cattggatgg aagtgcggaa agaattaact ccaatttcct tcatcaagtg gaaatcttcg
    23821 gtgtaagtat gaaaaaagtt actggctaca agaggtccga cattatcgaa aaaatcttct
    23881 ggatgcgttt tgaaccagta gtccatcaca ttttcatgta cttttccata ttgaccttct
    23941 gtctgaggtc cgctagaagc agctccccac caaaacagat taggaaatac taactttttt
    24001 ttcatgctca acctcctttc tattatgcta tacataaagt atagcataat tttaaaattt
    24061 gtctagacat ttttaaaaat tgttgataca tatattgtgt ttgcctcatt tcttgaactt
    24121 tgttatacta ctattagaat attatagaga gaaccagatt aaatgagtac aaaatattta
    24181 tttatttaca atgagattcg tgaaaagatt ctctgtaata aatataccat gaacgaacaa
    24241 ttgcctgatg aaatgacatt agctaaacag tttgcctgta gtcgaatgac gatcaaaaaa
    24301 gctttagact tgttagtttc tgagggctta atttttagaa aacgtgggca gggaaccttt
    24361 gttctctctc gtggcagctc aaaaagaaaa ttaatcgttc cagaaagaga tatccgggga
    24421 ctgacaaaaa tatctgaaga tgctcattct acaattgact cgaggattat tcacttcaaa
    24481 ttagaatttg caaatgaatt tttagcagaa aaactacagg tcgctttgca gagtccagtt
    24541 tataatattt accgcctgcg tattattgac ggtaaacctt atgttctgga acaaacttat
    24601 atgagtaccg atgttattcc aggtattact gaagatattt tacaaaaatc gatttacaat
    24661 tacattgaag gaaagttagg attgcatatt gccagtgcta caaaaatctt gcgagcttct
    24721 tctagttcag aaaatgagca acattacttg cagctccttc caacagaacc ggtatttgaa
    24781 gtagaacaag tggcttattt ggataacgga actccgtttg agtactcgat tagtcgtcat
    24841 cgctatgatt tatttgaatt taattctttt gcattacgac attcctccta ggagaaaatg
    24901 tgaaaatgaa gccaatcttt tacaggctct agtttaagaa aaatttaaaa cagggcaaga
    24961 aggtcccatc tatgcttaaa tggtttctct tttctaaata agatggcttt aaaagagtga
    25021 tcgttttatc catcatgttg aaaaatatct tcgtatagct tatagagtag gtactgaaat
    25081 tgttcacctg atctacttct tatagttatt tagttttaaa tagtgtttca aacattctta
    25141 cactgacgag aagtttttga gtcttttctt gtaacacata tagtatactg aggttagaat
    25201 agtagactgt gacttctaac aaattgctag aaatgaattt caatctccca atttatttgc
    25261 ttatatatta ttttaatata ttaaataaat tctggatcat aatcatttaa aaaaatttta
    25321 tttttttatt tttcattacg aataatatag atgaagggga aagagtatga aaacagaact
    25381 gtttcttttg ctattagttc aaaaggagaa aaaatgaaag tagaaaatat ttcgtatagg
    25441 gtggatcatc gtatattgtt tgataatatt tcttttgata cttcgagttc agacgtgaca
    25501 ttaattactg gtaaaaatgg tacaggaaag tcaactttac tatagtggat tgaaactaga
    25561 atagtacaca tctacttcta aaatattgtt agaaatcgat ttgactatcc tgatctattt
    25621 gtcctgttct tatttcattt cactatatct caaattgagt atgacgaagt gcgctcccat
    25681 gtcctgggaa cgcactttct tcatattttt catattcttg aatccatcga taaagactat
    25741 tgggatgaat ttttaaagtt gaactaatca tttttacagg atgaggttta cagcagagag
    25801 tttgaaggct ttatcaaagg tttttcttgg cataatgact tttcctcgtt tccacttaat
    25861 tttgtgtcta ctttattata ccaagtccac ccttaagtta gataataaat ctaacttaag
    25921 gaagctagaa ggatgagaat ccaggtggtc aagagtccca aacttaagct gattgggaca
    25981 cccagaataa tttgcttttt gaaggcaagg ccacgttcct ctatattggg aagtgagagt
    26041 tgaatgagag aaccagctga tgaaaagggt gagatattag tagatagagc gccaataacg
    26101 gtggctgttg tgagtaagtg aatatcaatc tggggatttt gagcactgat gatagcaatg
    26161 atgggaaaga gggctggagc tacaacggat agggtggaac taaagagtga catcaccccg
    26221 gctatcacac aaaagaacag aggtaaccag aaatgaggaa tggatgttgt catgaggtgc
    26281 cctatcagtg tgactaaacc tgacttgacc gctagagaca ttagtaagct catgccgcag
    26341 agcatgataa ttgtagccca gggaacttta gccaaaatgg cttcttgctt ccctaatttg
    26401 agccttaagg cgaggcagac catgagtatt gagacaaagc caatatcaaa tgtttttcga
    26461 taagtagcta tccaggcgat gtttgggaaa atgagatgca acaagggaaa aagccaaacc
    26521 aaaaccatgc tgctgatcat gagcaaggtg gtttgtcttt gaaccttgct gaggagtggt
    26581 ggttggtcaa tagtcaagga tgagtttgtt cttcccttac tatagtgact gtaacaggat
    26641 aataaaagca agacaatgag tgggtagata atgctgacga taaagatatg attgccaagt
    26701 gaaaaagctt gctcttccca tcccatttgc ttaaacaggc cttgaaagac aatgcctgag
    26761 ctactggtta tcaaattagc ccctcctgaa gctccccaat tgacggcttg agctccaatc
    26821 aaagggtgtt tgtccgcttt ttgacagaga gtaatcgcta gaggacagca aacggccata
    26881 gtagtgaaaa atccagcacc taaagcagac aaaagggttg ccatcaggta taaaatcatg
    26941 tagagggcgt tagggtaggt gcgtgtgcgg tagagaatgt gttgagccaa aacatcaaga
    27001 gtaccgttag ttattgcaaa gttataaaag agagagacgc taaaaatggt aaaaaagagt
    27061 gaggttggcc aaaaatgaag aagttctttg gggcttaatc ccatgagagt ggttgcgatg
    27121 aggtaagaaa aagcaatagc cagcaggcca atattgattt tggtgcggta accaattcca
    27181 atggctagag caatggcgct aatcattatt aaatgaatca ttgaattgtc ctttcagtta
    27241 gaatatggaa ataggttggc aattttggcg ctcaaaagac cacctgtttc ctattggtac
    27301 ttgactatta tacacaaaaa aaatacaatt gtcttgacaa ttgtattgac acttgttaga
    27361 attaaaaatg aggtattcag gcttgtgatt tcctatggaa gttaatagtg attgcctcta
    27421 atgcttacaa gtgatattaa aaatagagga cctagtgatg tcaatcattt caactgattt
    27481 aacccctttt caaatagatg atacattgaa agcagccttg cgagaagatg ttcattccga
    27541 agattacagt accaatgcca tttttgatca tcatggccaa gccaaggtgt cgctttttgc
    27601 caaggaagct ggtgttttag cggggctaac cgtttttcaa agggttttta ccctatttga
    27661 tgccgaggtg accttccaga atcctcatca atttaaggat ggggatcgtt tgactagtgg
    27721 cgatttggtt ttagaaatca taggctcggt gagaagtctc ttaacatgtg aacgcgttgc
    27781 cttgaatttt ttacaacatt tatcagggat tgcttcgatg acagctgctt atgtagaagc
    27841 cttaggcgat gattgcatta aggtatttga tactcgaaaa actactccta atttacgtct
    27901 ttttgagaaa tatgcagtga gagttggcgg tggctataat catcgcttta atttatcaga
    27961 tgctatcctg ctaaaagaca atcacattgc ggcagtaggt agtgttcaaa aggcaattgc
    28021 tcaagcgcgt gcctatgccc cttttgtgaa aatggtcgag gtggaagtgg aaagccttgc
    28081 tgctgccgaa gaagctgcgg cggcgggtgc tgatattatc atgttggata atatgtcatt
    28141 ggaacagatt gaacaggcca ttaccctaat tgcaggacgt tctcggattg aatgttctgg
    28201 aaatattgat atgaccacta ttagccgttt tcgtggttta gcgattgatt acgtctccag
    28261 tggtagttta acccatagtg ctaagagtct tgatttttcc atgaaggggt taacctacct
    28321 tgatgtctaa gttgtaaaat aaactaactt tttaaaggat gtctttcctc tagaacgagt
    28381 tttatgtcag atagtttaaa cgcctctt
//
LOCUS       DAVBCN010000021        28404 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVBCN010000021 DAVBCN010000000
VERSION     DAVBCN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28404
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.021"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            19..2172
                     /locus_tag="VQ497_000840"
     CDS             19..2172
                     /locus_tag="VQ497_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HET2517179.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTGINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2185..3534
                     /locus_tag="VQ497_000841"
     CDS             2185..3534
                     /locus_tag="VQ497_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET2517180.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAMETGASLASQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            3704..4411
                     /locus_tag="VQ497_000842"
     CDS             3704..4411
                     /locus_tag="VQ497_000842"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET2517181.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            4613..8338
                     /locus_tag="VQ497_000843"
     CDS             4613..8338
                     /locus_tag="VQ497_000843"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HET2517182.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVTKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8431..9873
                     /locus_tag="VQ497_000844"
     CDS             8431..9873
                     /locus_tag="VQ497_000844"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET2517183.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            9910..10932
                     /locus_tag="VQ497_000845"
     CDS             9910..10932
                     /locus_tag="VQ497_000845"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET2517184.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            10929..11474
                     /gene="purN"
                     /locus_tag="VQ497_000846"
     CDS             10929..11474
                     /gene="purN"
                     /locus_tag="VQ497_000846"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET2517185.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIALVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            11558..12067
                     /locus_tag="VQ497_000847"
     CDS             11558..12067
                     /locus_tag="VQ497_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET2517186.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLIFFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12092..13639
                     /gene="purH"
                     /locus_tag="VQ497_000848"
     CDS             12092..13639
                     /gene="purH"
                     /locus_tag="VQ497_000848"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET2517187.1"
                     /translation="MTKRVLISVSDKADIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYDDAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGENKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            13761..15023
                     /gene="purD"
                     /locus_tag="VQ497_000849"
     CDS             13761..15023
                     /gene="purD"
                     /locus_tag="VQ497_000849"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET2517188.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVSETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFFEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNITWTDKGVTLGVVVA
                     SKGYPLNYSKGVELPVKTDGDIITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            15426..15914
                     /gene="purE"
                     /locus_tag="VQ497_000850"
     CDS             15426..15914
                     /gene="purE"
                     /locus_tag="VQ497_000850"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET2517189.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            15901..16992
                     /gene="purK"
                     /locus_tag="VQ497_000851"
     CDS             15901..16992
                     /gene="purK"
                     /locus_tag="VQ497_000851"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET2517190.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTFCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17002..17229
                     /locus_tag="VQ497_000852"
     CDS             17002..17229
                     /locus_tag="VQ497_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HET2517191.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            17292..18590
                     /gene="purB"
                     /locus_tag="VQ497_000853"
     CDS             17292..18590
                     /gene="purB"
                     /locus_tag="VQ497_000853"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET2517192.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(18645..22583)
                     /gene="strH"
                     /locus_tag="VQ497_000854"
     CDS             complement(18645..22583)
                     /gene="strH"
                     /locus_tag="VQ497_000854"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET2517193.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEDDKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKESARTVNLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGSMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQTLNEVPKDLNRY
                     TAESVAAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVIEKKLPETGTHDSAGLVVAGLMATLAAYGLTKRKED"
     gene            complement(22898..23614)
                     /locus_tag="VQ497_000855"
     CDS             complement(22898..23614)
                     /locus_tag="VQ497_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2517194.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            23962..25749
                     /locus_tag="VQ497_000856"
     CDS             23962..25749
                     /locus_tag="VQ497_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HET2517195.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPVYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            25746..26222
                     /locus_tag="VQ497_000857"
     CDS             25746..26222
                     /locus_tag="VQ497_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET2517196.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            26250..27155
                     /locus_tag="VQ497_000858"
     CDS             26250..27155
                     /locus_tag="VQ497_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2517197.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            27142..27957
                     /locus_tag="VQ497_000859"
     CDS             27142..27957
                     /locus_tag="VQ497_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2517198.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            27964..28368
                     /locus_tag="VQ497_000860"
     CDS             27964..28368
                     /locus_tag="VQ497_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2517199.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
BASE COUNT         7744 a         5620 c         6710 g         8330 t
ORIGIN      
        1 agggaaaagg agatgaatat gaaatttggg aaacgtcact atcgtccgca agtggatcag
       61 atggactgcg gtgtagcttc attagccatg gtttttggct actatggtag ttattatttt
      121 ttggctcact tgcgagaatt ggctaagacg accatggatg ggacgacggc tttgggcttg
      181 gtcaaggtgg cagaggagat tggttttgag acgcgagcca ttaaggcgga tatgacgctt
      241 tttgacttgc cggatttgac ttttcctttt gttgcccatg tgcttaagga agggaaattg
      301 ctccactact atgtggtgac tgggcaggat aaggatagca ttcatattgc cgatccagat
      361 cccggggtga agttgactaa actgccacgt gagcgttttg aggaagaatg gacaggagtg
      421 actcttttta tggcacctag tccagactat aagccttata aggaacaaaa aaatggtctg
      481 ctctctttta tccctatatt agtgaagcag cgtggcttga ttgccaatat cgttttggca
      541 acactcttgg taaccgggat taacattgtg ggttcttatt atctgcagtc tatcattgat
      601 acctatgtgc cagatcagat gcgttcgaca ctagggatta tttctattgg gctagtcatc
      661 gtctacatcc tccagcaaat cttgtcttac gctcaggagt atctcttgct tgttttgggg
      721 caacgcttgt cgattgacgt gattttgtcc tatatcaagc atgtttttca cctccctatg
      781 tccttctttg cgacacgcag gacaggggag atcgtgtctc gttttacaga tgctaacagt
      841 atcatcgatg cgctggcttc gaccatcctt tcgattttcc tagatgtgtc aacggttgtc
      901 attatttccc ttgttctatt ttcacaaaat accaatctct ttttcatgac tttattggcg
      961 cttcctatct acacagtgat tatctttgcc tttatgaagc cgtttgaaaa gatgaatcgg
     1021 gataccatgg aagccaatgc ggttctgtct tcttctatca ttgaggacat caacggtatt
     1081 gagactatca agtccttgac cagtgaaagt cagcgttacc aaaaaattga caaggaattt
     1141 gtggattatc tgaagaaatc ctttacctat agtcgagcag agagtcagca aaaggctctg
     1201 aaaaaggttg cccatctctt gcttaatgtc ggcattctct ggatgggggc tgttctggtc
     1261 atggatggca agatgagttt ggggcagttg attacctata ataccttgct ggtttacttt
     1321 accaatcctt tggaaaatat catcaatctg caaaccaagc ttcagacagc gcaggttgcc
     1381 aataaccgtc taaatgaagt gtatctagta gcttctgagt ttgaggagaa gaaaacagtt
     1441 gaggatttga gcttgatgaa gggagatatg accttcaagc aggttcatta caagtatggc
     1501 tatggtcgag acgtcttgtc ggatatcaat ttaaccgttc cccaagggtc taaggtggct
     1561 tttgtgggga tttcagggtc aggtaagacg actttggcca agatgatggt taatttttac
     1621 gacccaagtc aaggggagat tagtctgggt ggtgtcaatc tcaatcagat tgataaaaaa
     1681 gccctgcgcc agtacatcaa ctatctgcct caacagccct atgtctttaa cggaacgatt
     1741 ttggagaatc ttcttttggg agccaaggag gggacgacac aggaagatat cttacgggcg
     1801 gtcgaattgg cagagattcg agaggatatc gagcgcatgc cactgaatta tcagacagaa
     1861 ttgacttcgg atggggcagg gatttcaggt ggtcaacgtc agagaatcgc tttggcgcgt
     1921 gctctcttga cagatgcgcc ggtcttgatt ttggatgagg cgactagcag tttggatatt
     1981 ttgacagaga agcggattgt cgataatctc atggctttgg acaagacctt gattttcatt
     2041 gctcaccgct tgactattgc tgagcggaca gagaaggtgg ttgtcttgga tcagggcaag
     2101 attgtcgaag aaggaaagca tgctgatttg cttgcacagg gaggctttta cgcccatttg
     2161 gtcaatagct agaaagagga gaggatgaaa ccagaatttt tagaaagtgc ggagttttat
     2221 aatcgtcgtt accataattt ttccagtagt gtgattgtac ccatggccct tctgctcgtg
     2281 tttttacttg gctttgcaac tgttgcagag aaggagatga gtttgtccac tagagctact
     2341 gtcgaaccca gtcgtatcct tgcaaatatc cagtcaacta gcaacaatcg tattcttgtc
     2401 aatcatttgg aagaaaataa gctggttaag aagggggatc tcttggttca ataccaagaa
     2461 ggggcagagg gtgtccaagc ggagtcctat gccagtcagt tggacatgct aaaggatcaa
     2521 aaaaagcaat tggagtatct gcaaaagagc ctgcaagaag gggagaacca ctttccagag
     2581 gaggataagt ttggctacca agccaccttt cgcgactaca tcagtcaagc aggcagtctt
     2641 agggctagta catcgcaaca aaatgagacc atcgcgtccc agaatgcagc agctagccaa
     2701 acccaagccg aaatcggcaa cctcatcagt caaacagagg ctaaaattcg cgattaccag
     2761 acagctaagt cagctatgga aacaggtgct tccttggcca gtcagaatct agcctactct
     2821 ctttaccagt cctacaagtc tcagggcgag gaaaatcccc aaactaaggt tcaggcagtt
     2881 gcacaggttg aagcacagat ttctcagtta gaatctagtc ttgctactta ccgtgtccag
     2941 tatgcaggtt caggtaccca gcaagcctat gcgtcagggt taagcagtca attggaatcc
     3001 cttaaatccc aacatttggc aaaggttggt caggaattga cccttctagc ccagaaaatc
     3061 ttggaggcag agtcaggtaa gaaggtacag ggaaatcttt tagacaaggg gaaaattacg
     3121 gcgagtgagg atggggtgct tcatcttaat cctgagacca gtgattctag catggttgca
     3181 gaaggtgccc tactagccca actttatcca tctttggaaa gagaagggaa agccaaactc
     3241 acagcctatc taagttcaaa agatgtagca agaatcaagg tcggtgattc tgttcgctat
     3301 actacgactc atgatgccgg gaatcaactt ttcctagatt ctactattac aagtattgat
     3361 gcgacagcta ctaagactga gaaagggaat ttctttaaaa tcgaggcgga gactaatcta
     3421 acttcggagc aggctgaaaa acttaggtac ggggtggaag gccgcttgca gatgattacg
     3481 ggcaagaaaa gttatctacg ttattatttg gatcaatttt tgaacaaaga gtaatgttcg
     3541 tgtttttaga gttaaataat ttttaaactg tgagaaagat tcttcttgca gttttttctt
     3601 tacaattttt aaaaaacatc tgctatttat tcggttaaat tcttgtgttt tttggttttt
     3661 tgtggtaaaa tgtgctcaag taatacgaaa ggcgaacttt aaaatgtcaa aacaattgat
     3721 ctattcggga aaagctaaag atatctatac aactgaggat gaaaatctta ttatttcaac
     3781 ttacaaggac caggcgactg ctttcaacgg tgtcaagaag gagcagattg caggtaaggg
     3841 agtcttgaat aatcagatct catcttttat ttttgagaaa ttaaatgcgg ctggtgtggc
     3901 gactcacttt gtggagaaac tttcagacac ggaacaactc aataaaaagg ttaagattat
     3961 tcctttggaa gtcgtgctcc gcaactatac tgctggttcc ttttcaaaac gttttggtgt
     4021 ggatgaggga atcgccttgg agactccgat tgtcgaattt tactacaaaa atgatgattt
     4081 ggatgatcca tttatcaatg atgagcatgt gaaattccta cagattgcgg gtgaccagca
     4141 gattgcctac ttgaaggaag aaacgcgtcg tatcaatgaa ctattgaaag tctggtttgc
     4201 tgagattggg cttaaattga ttgactttaa gctagagttc ggttttgaca aggatggcaa
     4261 gattatcttg gcagacgaat tttcaccaga taactgccgc ttgtgggacg ctgatggcaa
     4321 ccacatggat aaggatgttt tccgtagagg attgggagaa ctaaccgacg tttatgagat
     4381 tgtttgggaa aagttgcagg aattgaaata atctgtttgc aacggaaaac cttcgtctct
     4441 caactaaaag gactcaggct gaaaaggtcc cccagacctt ttcactctgt agagaactag
     4501 gtgaactaac agatgtttac gaaattgtct gggaaaagtt gcagggttta aaataacaac
     4561 ctcaaggctg tttgggaata ttgcaagagc tgaaataaag gaataagaat tgatggataa
     4621 acgtattttt gttgaaaaaa aggctgattt tcaggtcaag tcagagagtt tggttagaga
     4681 gctccagcac aacttgggac tgtcaagctt gaaaagtatt cgtattgtgc aagtatatga
     4741 tgtatttgac ttggctgagg acttgtttgc acctgcagag aagcacattt tctctgagca
     4801 gggaaccgac catgttttag atgaagtatc tgtgcaggcg gatcttgcta actatgcttt
     4861 ctttgccatt gaaagtctgc cagggcagtt tgaccagcgt gcagcttcgt cacaggaagc
     4921 cttgcttttg ttgggaagtt cgagtgacgt gacagtcaac acagcccaac tttacttggt
     4981 gaataaagat attgatgcga ctgagttgga agctgtcaaa aactacctgc tcaatccagt
     5041 tgattctcgt ttcaaggata tcacgacagg gattgccaag caggagtttt cagagtcaga
     5101 caagaccatt ccaaaattga ctttctttga aagctataca gcagaagact ttgctcgcta
     5161 caaggccgag caagggatgg ccatggaagt ggatgatttg ctctttatcc aagactactt
     5221 taagtcaatc gggcgcgtgc caactgagac tgaactcaag gttttggaca cttactggtc
     5281 tgaccactgc cgtcatacga cttttgagac agagttgaaa cacatcgact tttcagcttc
     5341 taaatttcaa aagcaattgc agtcaaccta tgacaagtat attgccatgc gcgaggagtt
     5401 aggtcggtct gaaaaaccac aaaccttgat ggatatggcg actattttcg gtcgttatga
     5461 gcgtgctaac ggacgattgg atgatatgga agtctctgac gaaatcaatg cctgctcagt
     5521 tgaaattgaa gtggatgttg atggtgtcaa ggaaccttgg ctcctcatgt ttaaaaatga
     5581 aacccacaac catccaacag aaattgagcc atttggtgga gcggctacct gtatcggtgg
     5641 agctattcgt gatccgttgt caggccgttc ctatgtttac caagccatgc gtatttcagg
     5701 tgctggtgat attacagcac cgatttcgga aactcgtgct gggaaattgc cacaacaagt
     5761 catttctaaa acagcagctc atggttattc ttcatatggt aaccagattg ggcttgccac
     5821 aacctacgtt cgtgaatact tccacccagg ctttgtagct aaacgtatgg aacttggtgc
     5881 cgttgttggt gcggctccca agggcaatgt tgtccgtgaa aaacctgaag caggtgatgt
     5941 gatcatcctt ctcggaggca aaacaggtcg tgatggtgtc ggtggtgcga cgggctcttc
     6001 taaggttcaa acagttgagt ctgtagagac tgctggtgct gaggttcaaa aaggaaatgc
     6061 catcgaagaa cgcaagattc agcgcctctt ccgtaatggc aatgtcactc gtctgatcaa
     6121 gaagtccaat gactttgggg caggcggcgt ctgtgtggct atcggtgaat tggcagacgg
     6181 tcttgaaatc gacctcaaca aggtgcctct taaataccag ggcttgaatg gtacagaaat
     6241 tgccatctct gaatcacaag aacggatggc ggtcgtggtt cgtcctgaag atgtggatac
     6301 cttcgttgcc gaatgtaaca aagaaaatat tgatgctgtt gtggtggcga cagtaactga
     6361 aaaaccaaat cttgtcatgc actggaatgg tgagacaatc gttgacttgg agcgtcgttt
     6421 ccttgacacc aatggtgtgc gcgtggttgt cgatgccaaa gttgtggaca aggatgtcaa
     6481 actcccagaa gagcgtcaaa catctgctga aacactggaa tcagataccc ttacggttct
     6541 atctgacctc aaccatgcaa gtcaaaaagg attacagact atctttgact gctctgttgg
     6601 acgctcaacg gttaatcacc cacttggtgg tcgttaccaa ctcacaccaa ctgaggcatc
     6661 tgtgcagaaa ttgccagttc aacacggtgt gactcatact gcgtcggtca ttgctcaagg
     6721 tttcaaccca tatgtagctg aatggtctcc ataccacggt gctgcttatg cggttatcga
     6781 agcaactgct cgtttggtgg ctgctggtgc caactggtct aaggctcgtt tctcttacca
     6841 agaatatttc gagcggatgg acaagcaagc agagcgtttc ggtcagccag tagctgctct
     6901 tctaggttct attgaagcac aaattcagct tggcttgcca tctatcggtg gtaaggactc
     6961 catgtctggt acctttgaag aattgaccgt tccgccaacc ttggttgcct ttggggtgac
     7021 gacggcagat agccgtaagg tgctctctcc agaatttaaa gctgttgggg aaaatatcta
     7081 ctacatccca ggtcaagccc tctctgcaga gattgatttt gacttgatta agaaaaattt
     7141 tgctcagttt gaagcaagcc aagctgacca taaagtgacg tctgcatcag ctgtcaaata
     7201 cggtggtgta gttgaaagtt tggctcttgc tacctttgga aactatattg gtgcagaggt
     7261 gaccttgcct gaacttaaaa cagctttgac agctcaatta ggcggctttg tcttcacatc
     7321 tcctgaagaa attgctggag tagagaagat tggacaaacg aaagcagact ttacactgac
     7381 tgtcaacggt gtgaagctag atggacacaa gcttgacagt gcatttcaag ggacattgga
     7441 agaagtttac ccaacagaat ttacccaagc gaaagaacta gaagaagtac cagctgtggc
     7501 atcagatgtt gtgattaaag ccaaagaaaa ggttgaaaaa cctgtggttt acatcccagt
     7561 ctttccagga accaactcag aatatgattc agctaaggcc ttcgaaaaag aaggtgcaga
     7621 ggtcaatttg gtgccattcg tgaccttgaa tgaagaagct attgtcaagt cagttgaaac
     7681 tatggttgac aatatcgaca agactaatat tctcttcttt gccggtggat tctcggctgc
     7741 ggatgaacca gatggttcag ctaagtttat cgtcaatatc ctgcttaatg aaaaagtgcg
     7801 tgtggctatt gatagcttta tcgcccgtgg tggcttgatt atcggtattt gtaatggatt
     7861 ccaagcctta gtcaaatcgg gtctcctacc atacggaaac tttgaagatg ctaacagtac
     7921 tagtccaacc ctcttctaca atgatgccaa ccaacacgtg accaagatgg tggaaactcg
     7981 cattgccaat accaactcac catggttggc tggtgtgcaa gtgggcgata tccacgctat
     8041 tcctgtttcg cacggtgaag ggaagtttgt cgtgacggct gaggaatttg cagagctccg
     8101 tgacaatgga caaattttca gccaatacgt tgactttaac ggtaaaccaa gtatggattc
     8161 taagtacaat ccgaatggtt ctgtccatgc catcgaagga attaccagca agaatggtca
     8221 aatcatcggt aagatgggcc actcagaacg ttatgaggat ggtcttttcc aaaatatccc
     8281 aggcaataaa gaccaacacc tgttcgcatc agcggttaaa catttcactg gaaaataaga
     8341 cttacagatt ttctaataga tagtatcagt aatgtaaaag tcatgtaaat ctagctcttg
     8401 atgattacaa atgaaaatta ggtataaaaa atgacatacg aagtaaaatc tcttaatgaa
     8461 gaatgtggtg ttttcggtat ttggggacat ccagatgctg ctaagttgac ctattttgga
     8521 ctccacagtc ttcaacaccg tggtcaggag ggggcaggaa tcctctccaa tgatcaagga
     8581 caactgaagc gccatcgtga catggggctt ttatcagaag ttttcagaaa tccagccaat
     8641 ttggataaat tgacaggagc tggtgcgatt gggcatgtgc gttatgcgac tgctggcgaa
     8701 gcttctgttg ataacatcca gcccttcctc ttccgttttc acgatatgca gtttggcttg
     8761 gctcataatg gaaatctgac caatgcagcc tctctcaaga aagaactgga acaaagagga
     8821 gcaattttca gcgcgacttc ggactctgaa atcttggctc acctcattcg tcgtagtcat
     8881 aatcctagcc tgatgggcaa aatcaaggaa gcgctcagcc ttgtcaaagg tggttttgcc
     8941 tatatcttgc tgtttgagga caagttgatt gcggctcttg accctaatgg ctttcgtccg
     9001 ctttctatcg ggaaaatggc caacggagcg gtggttgttt cctctgaaac ctgtgctttt
     9061 gaggtcattg gtgccgagtg gattcgtgat ttgaagccag gtgagattgt gatcattgat
     9121 gacgagggca ttcagtatga cagctataca gatgatactc agttggcgat ttgttctatg
     9181 gagtatatct actttgctcg ccctgattct aatatccacg gtgtcaatgt ccatacggca
     9241 cgtaagagaa tgggagcgca attggcgcga gaatttaagc atgaggcaga tattgtagtt
     9301 ggtgtgccca attcttccct aagcgcggct atgggatttg cggaagaatc aggcttacca
     9361 aatgaaatgg gtctgatcaa aaaccaatac acccagcgaa cttttatcca accgactcaa
     9421 gaattgcggg agcaaggagt gcggatgaaa ctgtctgctg tttcgggtgt tgtcaaaggc
     9481 aaacgtgtgg tcatggtgga tgattccatt gtacgtggaa caacctctcg tcgtatcgtt
     9541 cagctcttga aagaagcggg tgcgactgag gttcacgttg ccattggaag tcctgcacta
     9601 gcgtatccat gtttctacgg gattgatatc cagacccgtc aggagctgat tgcagccaat
     9661 catacggtcg aagaaactcg ccaaatcatt ggtgcggaca gtctgactta tctttcaatt
     9721 gatggcttga ttgagtcgat tggtatcgaa acagatgcgc cgaacggtgg tctctgtgtc
     9781 gcttactttg acggtgacta cccaacgcct ctctatgact acgaagaaga ctatcgtaga
     9841 agtttggaag aaaagaccag tttttacaag taggcgacag attctccatt aaagaaaagg
     9901 aaaaaacaaa tgacaaataa aaatgcatat gcccagtcgg gtgtggatgt tgaagcgggt
     9961 tatgaagttg ttgaacggat taaaaagcac gtggcccgta cggagcgtgc aggtgtcatg
    10021 ggagctcttg gtggctttgg tggtatgttt gacctttcca agactggggt taaagaaccc
    10081 gtcttgattt cagggactga cggtgtcgga accaagctca tgttggctat caagtacgac
    10141 aagcacgata ccatcgggca ggactgtgtg gccatgtgtg tcaacgacat cattgctgca
    10201 ggtgcggaac ccctctattt tctcgactac gtagcgacag ggaagaatga accagctaag
    10261 ctagaacaag tggttgctgg tgtggcagaa ggttgtgtgc aggctggtgc tgccctcatc
    10321 ggtggggaaa cggctgaaat gccgggcatg tacggcgaag acgactatga cttggctggt
    10381 tttgcggtcg gtgtggctga aaaatctcaa atcattgacg gttcaaaggt ggtagaggga
    10441 gatgttcttc tcggacttgc ttcaagtggg attcactcaa atggttactc tttggttcgt
    10501 cgtgtctttg cggattacac aggtgaggaa gtcctaccag aattggaagg caagaaactt
    10561 aaggaagttc tacttgagcc gactcgtatc tatgtcaagg ctgtcttgcc gctcatcaaa
    10621 gaagagttgg tcaacggcat tgcccacatc acaggtggtg gctttatcga aaatgtccct
    10681 cgtatgtttg cagatgacct agctgctgaa attgatgaaa gcaaagttcc agtgcttcca
    10741 attttcaaag cccttgaaaa atatggtcag attaaacacg aagaaatgtt tgaaatcttc
    10801 aatatgggtg tgggacttat gttggcggtc agccctgaaa atgtagagcg tgtaaaagaa
    10861 ttgttggatg aagcagtcta tgaaattggt cgcatcgtca agaaagaaaa cgaaagtgtc
    10921 attatcaaat gaaaaaaata gcggtttttg cctctggtaa tggctcaaat tttcaggtga
    10981 ttgccgaaga atttccagtg gagtttgtct tttcagacca tcgtgatgcc tatgtgcttg
    11041 agcgtgcaaa gcagctcggc gttctgtcct atgcttttga actcaaggag tttgagagca
    11101 aggcagacta cgaagcagcc cttgtcgaac tcttggaaga acaccagatt gccttggttt
    11161 gcctagcagg ctacatgaaa atcgttggac caaccttatt gtcggcttat gaaggtcgga
    11221 ttgtcaacat tcatccagcc tacttgccag aatttccagg agctcatggg attgaggatg
    11281 cttggaatgc tggcgtgggt cagtctggtg tgaccattca ctgggtggat tcgggtgtgg
    11341 atacaggcca ggtcatcaaa caggttcgtg tgccacgact agctgatgat accattgaca
    11401 gatttgaagc tcgcatccat gaagcagagt acaggttgta tccggaagta gtgaaggctc
    11461 tatttacaga ttgacttttt gatgattcat atgatatctt tgattttaaa ttggagtcag
    11521 tgtttgttga agacggcttc aaacggaggt atttgtaatg ttagaatcta aaaaaacaac
    11581 tcgatatgta ttttatgtct atctgatgtt attaacttgg ggaatcttat ttaagtttga
    11641 aacaaatcct gaatttatag catttttctt agctccaagg tatatcaatt ggattccatt
    11701 ttcagaacca ctaatagtcg atggaaaaat tgtttttgct gaaatgttat ttaatctgat
    11761 tttctttatt ccattaggtg tttgtttccc tttgataaaa actaatttat ctagtttaag
    11821 aatagtcggg acaggtttct tgattagttt attgtttgag tgcttacagt atattttagc
    11881 aataggtata acagatataa cggatttgac tttaaatacg ctaggtgtct gtgtaggctt
    11941 actgatttat caaattttta taagagtgtt caaatcacag actagaaaat ggatcaatat
    12001 cttaggtatg cttagccttg gttttgctta tcttgtttta ctgttactgc atttaattgg
    12061 tgtttaacta atgattaaaa aggagaatat aatgactaaa cgcgtcttaa tcagcgtctc
    12121 agacaaagcg gacattgttg aatttgccca agaactcaaa aaacttggtt gggagattat
    12181 ctcgacaggt ggaactaagg ttgcccttga taatgctggg gtggatacca ttgctatcga
    12241 tgatgtgact ggtttcccag aaatgatgga cggtcgtgtg aagaccctcc acccaaatat
    12301 ccacggaggg cttctcgctc gtcgtgactt ggatagccac ttggaagcgg ctaaggacaa
    12361 caagattgag ctcattgacc ttgtggtggt caacctttac ccatttaagg aaactatcct
    12421 taaaccagat gtgacttatg atgatgcagt tgaaaatatc gatattggtg ggccatctat
    12481 gcttcgttca gcagcgaaaa atcatgccag tgttacagtt gtggtagatc ctgctgacta
    12541 cgctgtggtt ttggacgaat tggcagcaaa cggcgaaacc tcttatgaaa ctcgccaacg
    12601 tttagcagcc aaagtatttc gtcacacagc ggcttatgac gccttgattg cagaatactt
    12661 cacagctcaa gtgggtgaaa acaagcctga aaaactcact ttgacttatg acctcaagca
    12721 accaatgcgt tacggtgaga atcctcaaca agacgcggac ttttaccaga aagctttgcc
    12781 tacagactac tccattgctt cagccaaaca gctcaacggg aaagaattgt catttaataa
    12841 tatccgtgat gcagatgctg ctatccgtat catccgtgac ttcaaagata gtccaaccgt
    12901 tgtggctctc aaacacatga atccatgtgg aattggtcaa gctgatgaca tcgagactgc
    12961 ttgggactac gcttatgagt ctgacccagt gtctatcttt ggtgggattg tcgtcctcaa
    13021 ccgtgaggtg gatgctgcga cagctgagaa gatgcacggc gttttcctcg aaatcatcat
    13081 tgcaccaagc tatacggatg aagcgctagc cattttgatc aataaaaaga aaaacttgcg
    13141 tatccttgcc ttgccattta atgctcaaga ggctagcgaa gtggaagcag aatacacagg
    13201 tgtagtcggt ggacttctcg tgcaaaatca agacgtggtc aaggaaagcc cagctgactg
    13261 gcaagtggtg actaaacgtc agccaactga gacagaagcg actgctcttg agttcgcttg
    13321 gaaggctatc aagtacgtca aatcaaatgg tattatcgtg accaacgacc acatgacact
    13381 tggtgttggt ccaggtcaaa ccaaccgtgt ggcttctgtt cgccttgcca ttgaccaagc
    13441 caaagatcgt ctggacgggg cggtccttgc ttcagatgcc ttcttcccat ttgcggataa
    13501 cgtggaagaa atcgccaaag caggaattaa ggccatcatc cagcctggtg gctctgtccg
    13561 tgaccaagaa tccatcgaag cagcggataa atacggcttg actatggtct ttacaggtgt
    13621 gagacatttt agacattaag aagataaaag ggaagaaaac agtttctttc cttttttggc
    13681 ttaaaatgct aactgaaaca agattaaaac gaactttttt gatataatgt tggtaaataa
    13741 ttcgcaaaag aggttgagga atgaaactgc ttgttgtcgg ttctggtggt cgtgagcatg
    13801 cgattgctaa aaagttactt gaatcaaaag acgtggaaaa agtctttgta gctcctggga
    13861 atgatgggat gactctggat ggtttggaat tggtaaatat ctctatttcc gaacattcta
    13921 aattgattga cttcgcaaag accaatgatg ttgcttggac ctttatcggt ccagatgatg
    13981 cccttgctgc tggtatcgtg gatgatttta accaagctgg gcttaaggcc tttggtccga
    14041 ctagggctgc agcggagctg gagtggtcca aggattttgc caaggaaatc atggtcaaat
    14101 atggtgtttc gacagcagcc tatggcacat tttcagattt cgaggaagca aaggcctata
    14161 tcgaaaagca tggtgcgcct atcgtagtca aggcggatgg cttggcgctt gggaagggtg
    14221 tcgtcgtttc tgagacggtt gagcaagcgg tcgaagccgc tcatgagatg cttttggaca
    14281 ataaatttgg tgactcaggt gcgcgcgtgg ttattgagga attctttgaa ggagaggaat
    14341 tttcactctt tgcctttgtc aatggtgata agttctacat catgccaacg gctcaggacc
    14401 acaaacgtgc ctatgatggc gacaaagggc ctaacacggg tggtatgggt gcctatgcgc
    14461 cagttccaca cttaccacag agtgtagttg atacagcggt tgacaccatt gtcaagccag
    14521 ttctagaagg gatgattaaa gaaggtcgcc cttatctggg agttctttac gcagggctta
    14581 tcctgacagc tgatggaccg aaagtcattg agttcaacgc tcggttcgga gatccagaaa
    14641 ctcagattat cttgcctcgc ttaacctctg actttgctca aaatatcaca gatatcctgg
    14701 atagcaagga gccaaatatc acgtggacgg acaagggtgt gactctgggt gtggttgtcg
    14761 catccaaggg ctacccgcta aattattcaa agggtgttga gttgccagtc aaaaccgatg
    14821 gcgacatcat tacctactat gcaggggcta agtttgcgga aaatagcaga gcactgctct
    14881 caaacggcgg acgagtttat atgctcgtta ccacagcaga taccgtcaaa gaagcccaag
    14941 ccagcatcta ccaagaacta tcccaacaaa aaatagaagg actcttctac cgaacagata
    15001 tcggaagcaa ggcaattaag taaagatata agaataacgc gccgtagtcg ccaaacacga
    15061 taatggtcgt cgtggtgaaa agaccagaac agtgaatgtt ctggtcaggg ggaaacttgg
    15121 agaccgtagg ctcaaagttt aggaatgaaa ccgaaggttt gcttccgcct ccatcaccta
    15181 agaccattat caaaaagaaa aataaaaatt cacaaaatac gttaatgatc gtatggtttg
    15241 cgagcgttag cgagctaata tagaacaatc accgccgttg tgaaagaacg attggatgat
    15301 aatccaatcg ttcagggaaa ttggaagacc ttgggtttcc aatttaggca tgagacacct
    15361 ttggtggctg ctgccgtccc tcacaagcta aggtgattgt tgaaaaagag gaaaaaggag
    15421 aagaaatgaa accagtaatt tccatcatca tgggctcaaa atccgactgg gcaaccatgc
    15481 aaaaaacagc agaagtccta gaccgcttcg gtgtagccta cgaaaagaaa gttgtttccg
    15541 cacaccgcac accagacctc atgttcaaac atgcagaaga agcccgtagt cgtggcatca
    15601 agatcatcat cgcaggtgct ggtggcgcag cgcatttgcc aggcatggta gctgccaaaa
    15661 caacccttcc agtcattggt gtgccagtca agtctcgtgc tcttagtgga gtggattcac
    15721 tctattctat cgttcagatg ccgggtgggg tgcctgttgc gaccatggct atcggtgaag
    15781 ctggagcgac taacgcagct ctctttgccc tccgtctcct ctctgtagaa gataagtcca
    15841 ttgcggatgc acttgccaac tttgctgaag aacaaggaaa aatcgcagag gagtcgtcaa
    15901 atgagctcat ctaaaacaat cggaattatc ggtggcggtc aactgggtca gatgatggcc
    15961 atttctgcta tctacatggg ccacaaggtt atcgcgctgg atcctgcggc ggattgcccg
    16021 gcctctcgcg tggcggaaat cattgtggca ccttataacg atgtggacgc cctccgtcag
    16081 ttggcagacc gttgcgatgt cctcacttat gagtttgaaa atgtcgacgc tgacggtttg
    16141 gatgccgtta tcaaggatgg acaactccct caaggaacag atctgctccg catttcgcaa
    16201 aatcgtattt ttgaaaagga ctttttgtca aacaaggctc aagtcactgt ggcaccctac
    16261 aaggtcgtga cttctagcct agacttggca gatatcgact tgtcgaaaaa ctatgtcctc
    16321 aagactgcga ctggtggcta cgatggtcat ggacaaaagg ttattcgttc agaagcagac
    16381 ttggaagcag cctatgcgct agcagactca gcagactgcg tcttggaaga atttgtcaac
    16441 tttgaccttg agatttctgt catcgtgtca ggaaatggca aggaggtgac gtttttccca
    16501 gttcaggaaa atatccaccg caacaatatc ctgtctaaga ccatcgtacc agcccgcatt
    16561 tctgaaagtc tagcagacaa ggctaaagct atggcagtgc gaatcgcaga acaactcaac
    16621 ttgtctggaa ctttctgtgt ggaaatgttt gcgactgatg atgacgttat tgtcaatgaa
    16681 atcgccccac gaccacataa ctctgggcac tattctattg aagcctgtga tttctctcag
    16741 tttgacaccc atattctggg tgttctcgga gcaccattac cagtcatcaa actccatgcg
    16801 ccagccgtta tgcttaatgt cctcggtcag catgtcgagg ctgctgagca atatgtgaca
    16861 gaaaattcaa gcgcccacct ccacatgtat ggtaaaatag aagcgaagca caaccgcaag
    16921 atgggacatg tgaccttgtt tagtgatgta ccggatagtg tggatgagct tggggaaggg
    16981 attgattttt aggacaagtc tatgatacaa attatcgtta atacatttat tgaaaaggat
    17041 aagactggag cagttgttga agtgttgtat gccagtgctg accaagataa ggtacaagct
    17101 aaatatgaag aactagctgc acaatacccc gaaaattatt tagctatcta tgatgtaccg
    17161 ctggatacgg atttgaatac actagatcat tacccgtctg tgtttattga aaaagaggag
    17221 tttgagtaga aatcttggtt tacctagata gcttattccc aacagcttaa gaagaaagga
    17281 aaaattaaca catgatcaac cgttactctc gccctgagat ggcgaatatt tggagtgaag
    17341 aaaataaata ccgtgcttgg cttgaggtgg aaatcctctc tgacgaggca tgggctgagt
    17401 tgggggaaat ccctaaggaa gatgtggctt tgattcgcaa gaaggcggac tttgacatcg
    17461 accgtatttt ggaaattgag caggagacgc gccacgatgt ggtggctttc acgcgtgcgg
    17521 tttctgagac tcttggtgaa gagcgcaagt gggttcacta tgggttaact tctaccgacg
    17581 tggtggatac tgcttatggt tacctctaca agcaggccaa cgacatcatc cgtcgtgacc
    17641 ttgaaaactt cactaatatc atcgctgaca aggccaagga gcacaagttc accatcatga
    17701 tggggcgtac tcatggtgtg cacgctgagc cgacaacctt tggtcttaaa ttggcaactt
    17761 ggtacagcga aatgaaacgc aatatcgagc gtttcgagca tgcagctgct ggtgtagaag
    17821 ctggtaagat ttctggtgcg gttgggaact ttgccaatat cccaccattt gtagaggagt
    17881 atgtctgcga taaacttggc atccgtgccc aagaaatctc tacacaagtc cttcctcgtg
    17941 accttcacgc tgagtacttt gcggttcttg ccagcattgc gacttcaatc gaacgtatgg
    18001 cgactgagat tcgtggtcta caaaaatctg agcaacgcga agtagaagag ttctttgcta
    18061 aagggcaaaa agggtcttca gcaatgcctc acaaacgcaa cccaatcggt tctgaaaata
    18121 tgactggtct ggcgcgtgtc attcgtggtc acatgattac ggcttatgaa aacgtcgctc
    18181 tctggcacga acgcgatatt tctcactcat cagctgagcg tatcatcaca ccagatacga
    18241 ccattttgat tgactacatg ctcaaccgtt ttggaaatat cgtcaagaac ttgacagtct
    18301 tcccagaaaa tatgatccga aacatgaact cgacttttgg tcttatcttt agccaacggg
    18361 ctatgttgac attgattgaa aaaggcatga cccgtgagca agcctatgac ttggtgcaac
    18421 caaaaacagc ctactcttgg gacaaccaag tagactttaa accacttctt gaggcagatt
    18481 cagaagtaac atcacgtctc actcaagaag aaatcgatga aatcttcaac ccagtttatt
    18541 acaccaaacg agtggatgat atctttgaac gtcttggact aggtgattaa ttaaaaaata
    18601 aacagcgagc ttcaatctcg ctgtttattt tttatcgaaa agacttagtc ttcttttctt
    18661 ttagtgagtc cataggctgc tagtgtggcc atgagtcctg cgactactag tcctgcagaa
    18721 tcgtgagttc ctgtttcagg aagttttttc tctattacca caggagctgg atcttgagga
    18781 agagctttgc tttcctcagc aggagcagtt gatggagctg gttggcttgg gatttctagt
    18841 tttggttttt cttcagcaat agcggcttgt ccgttttcat cgcctacatg tgttaccata
    18901 gttccgactt cgactatttg agtaacggct tcctgtgcta cgacactatt tacaagtgtt
    18961 ttcacttcct taccatcggc agaagtgctc acagagtaga agttgctacg atgtccattg
    19021 acgcccttag taatgacttg tgtttttcct ttgagtaaga gtggattttc acgagtcact
    19081 gtggtaaatg gaatttcttc tttttggata tccagtctag gttttacctc agtagttggt
    19141 gcaagaccac tttcatcacc cttgtgagtt acaggagcgc caacttcaac cacttggttt
    19201 ataacttctt tggttacctg gctatcaagg actgtttctg ttgtttttcc attttcagtg
    19261 agtacagaga tgtaatgagt tcgctcacct ttgactcctg ctgtgataat attttcctga
    19321 ccagctggga ggttaggatt ttctttcttg ataacttcaa atggaatttc ttcagttctt
    19381 gtgatgagtt ctggtctggt ttcaacattg gcagccactt cattttcatc caggcttcct
    19441 gaatgagttg cagctggttt gaggccttga agagcggctt ttaggttggc tacaagtgtg
    19501 tcaagctcag cttgtttatt acggttgagg ttgtaattta gagctgtttt agctgcgtca
    19561 agggcctcaa gactttcttt actatatcct tctaagtttg taggaatttt agctaattct
    19621 tcgcggagag cattataatt agcacggaag tagtctttgt tgtggtctgc aaaggcagtc
    19681 atgagttcaa agatttcctc ttccttgtat tcagcgcttg gtctatctgc ccagattgaa
    19741 agcatacttc cgactgttgg aagatctact tcaggatatt tggtagaagc tagttgattg
    19801 aatggtgttt ttccagtatt ctcaatagct ttcttgagga aaccaccacc atcttctggt
    19861 ttttgaccaa gaatgtagta ccagtcaccg ttggtattca agaatttata gcctttgctt
    19921 gctaggtatt gaggtgatgc gaggttatat ccccaccagc ctttagacca gtaagaaatc
    19981 aagacatctt tgtcaaactg aacatcgtcc ttgtcttcat agtagaagcc atcgttgaag
    20041 gccattggtt gaagccctct ttctttggcc atagctgcga gggtgttggc atattcggca
    20101 aatttgccat agagttgata ccacttgagg tagtaccagc cttgggcact agtcgcatcg
    20161 ttggcgtatt cgtcagtacc aaagttgaaa atctttgttt tacctgcaaa gaagtccatg
    20221 tatttaccga tgagggcttt tacaaagttc atcgcttctt cgtttttcaa gtccatagtt
    20281 gtttttgaaa ctttatcaaa gtgggcttga ggatttttaa tacctaattt ttccatggca
    20341 accagcatag catccatgtg acctggactg ttaatagctg ggatgagacc gatgtcctta
    20401 gatttagcgt attcaattag ctctgttact tctgcctgtg ttagtgcagt accgtttgga
    20461 tcgtcgtagt aagctttagt tccttcgata atagcttttt taacgtcatc actagcatag
    20521 gtttttccgt tggcagtaat ggtcatatca tcgagtagaa agcgaagtcc gtcatttcct
    20581 agaaggagat ggacatcaga atatccgagc tcactggcct tgtctacgat gcgtttgagc
    20641 tggttcagag taaagtattt gcgtccagca tcgattgaga ttaccttgtt tttggcaagt
    20701 ttttcaacct cacgtttagc ttcttcttct ttttgagctt caggcgtgag ggtcaagttg
    20761 ttgacagttt cttgaagttt agcaatggct tgatcaatcg tatcttgttg ggcacggcta
    20821 aggttgctat cgagagagcg aatagctttt tcagcttctt ttacggccgc gacgctttct
    20881 gcagtataac ggttcaggtc ttttggtacc tcgttaagtg tttgctctgc agattcataa
    20941 tcagctgcga agtattcagc gttggcattt gcaaaatgac gcatgagttt gaagaggcgt
    21001 gatggtgaat aacgtgcaga tggagtgtca gcccaagcag ctaccatact accgatgatt
    21061 gggatatcag ctccttctgt ttttggtaca gaagtgattg gtgtgttttt aataccattg
    21121 agcccctgat cgagattgta ccagccttgg ccatcagcgt ttcgtccaag aacgtagtac
    21181 caagcatcat tggtattaag gatttggtga cctttttcag ctagtagttt agaagaagcg
    21241 acatcgtagc ctccccaacc accagtccac atagaaacga tgatgtcttt gtcaaaacta
    21301 ccaaagcttg tgtcgctatt gtagtagata ccgtcgttaa aggccattgg tttgagaccg
    21361 tgcgatttta caatacgagc gaggtcattg gcgtaggcaa taaatttttc atagcctttt
    21421 acagggtagc cttcgtttgg atagtattta tcagcttgaa gcacactcca acctttagca
    21481 tctgtcgcat cattggcata ttcatcaagt ccgatgttga agatttcagt ctttttcgcg
    21541 aaataagcag catacttgtc gataagggct tttgtaaaag cgacagcttg ttcgttgtca
    21601 agattgacag tacgggctga ttccttccca aaatagctaa agttagggtt ttggattccc
    21661 aattctttca tggcattgag aatcgcatcc atgtgaccag gactatttac tgtcggaatg
    21721 agaccgatac ctttatcttt ggcatagtta atcagatctg tcatttgact ttctgttaag
    21781 tgattgccgt ttggatcgtt gtaataatca tttgtacctt tttcaatggc gcgtttgaca
    21841 tcgtcactgg cataggtctt gccgttagct gtgatgctca tatcgtccaa catgaaacgg
    21901 agtccatcat ttccgactaa taggtgtaaa tcagtgtagc cataatgttt cgctttatcg
    21961 atgatttcct tgagctgttc tggtgagaaa tatttacgtc cagcatcaat agaaacaatt
    22021 ttctttttcg ctagtttttc atttacagtt gcagcacgtt cctttcctgc ctctgtttcc
    22081 ggtttgtcat cctctgcttt cgcttcatct tttttagctg gtttatcctt gtcagtcttg
    22141 tctgtatttg actctttaga atcaacctct ttcgcttctt cctttttagg gctagcttct
    22201 tctgcctttt tattagcagt ttctttttca gcagaagttg gagttaccac ttctgcttta
    22261 tcactaggag ttgaactaac ttcctcttgt ggtttttctt ctgtttttgg aagactagct
    22321 accttatcag tagctggagt ttctgtttct acagtttttg gagcttctgg ttgaagctct
    22381 gctttaggtg tttcctcagt ccgattttcg gatgattgag gggaatcaga aaccgtatgg
    22441 atggtcggtt ggttttctgt agtagtagga gtaactccat cggctgcaac agtctgtgct
    22501 tggaaggcaa atccaattag aacagaagct gctcctacag cgtatttacg aatagaaaaa
    22561 cgctgttgtt tttcatgttt cattgcaaaa cctcctgatt gcattgttat attgatagcg
    22621 attatataaa tcaacgcctt tattttattt cttatattaa cgagagtcaa gaggagatga
    22681 caaaaaacta taataagtat aaaaaaatat aaaatttaaa cttaagattt cagattggtc
    22741 ggaaaaaata cgtatatata tctagtataa tttttggttc tatttctata aaatattcca
    22801 caaattatag aattttccaa aaataggtaa gcgctacctt tttggtgtgg tataataagc
    22861 atagaaaaag cccaagcgat tagctcaggc tttcttctta gtgatcacgg tcacatgaga
    22921 taaatttaat cttgtagtaa tcagatcgtt tgtaagtttc actgtattct aaaacttggc
    22981 cagttgattc gagtttggtg attttagttt gtaggacagt agggaattgt tcatcgactc
    23041 cgaggactga agccgcatgt tctggagttg gaaagactat ttcgttgatt tcttcaaagt
    23101 gttcatcatt catgtgaatg tggtagtcta acttgaaacg attatagata gaactatagt
    23161 attcaaggtt tggataattt gcgttgatat attgttctgg gatgtaggat gtatggtaga
    23221 tataaacgac accgtttgat tcgcggatac gttcaatctt gtagtagaat tgatcgccgc
    23281 gtagacccaa tttttccaag taaacaagct tgtttccgcg ttcaattgaa agaacagtta
    23341 ccttatcatc tttagcattg aagagttcaa tatctgaaaa ctctacaagc ttgtgtttgc
    23401 gtgcacgtga aacgaaggtt ccttttcctt gttggcggac aatatagcca tctttggcaa
    23461 ggtcgtttaa ggcgcgaaca actgtgatag agctgacatc gtacattgaa atgagttctg
    23521 cttcagtgta aaatttatct ccactgctaa actgcccaga gatgatttta ttttttaatt
    23581 cgtcttttat gtattgatat ttgggaattg ccatattttt acctctattt tccttctcca
    23641 taggaaattt taccataaaa gcgaaaaaaa tagtagtctt ttgaataaaa aattagaata
    23701 atagtttgaa aaattaagcg atatagggat tgctcatatt ttatcaatgt attctataca
    23761 aaattatttt aggtcccata ttccgaattt aaagagcgtt tgaaatgggt ctaaaaatgt
    23821 ttgaaactaa ttgaatgtgt gtataatagt aaaaatgcag taaatatccc gacaatttct
    23881 attgacaatt caaacagatt ggtttataat taatataaca acaaatgaaa gcgcaaactt
    23941 tcgcggtcgg aaggtagttt tatgacacga tttgagatac gagatgattt ctatctcgat
    24001 ggaaaatcat ttaagatttt atctggtgcc attcattatt ttagggttcc tccagaggat
    24061 tggtatcatt cgctctataa cttgaaggct cttggtttta atacggtaga gacttatgtt
    24121 gcttggaatt tacacgagcc tcgtgaaggt gagtttcatt ttgaaggtga tctggattta
    24181 gagaaatttc tccaaatagc gcaggatttg ggtctctacg caattgtgcg tccgtctcca
    24241 tttatctgtg cggaatggga attcggtggc ttaccagctt ggctcttgac caagaacatg
    24301 cgaattcgct catccgaccc agtatatatc gaggcagttg gtcgctacta tgatcagtta
    24361 ttgccaagac tggtgcctcg tttgttggac aatggtggca atattctcat gatgcaggtt
    24421 gaaaatgagt atggttctta cggagaagat aaggcttacc tgagagcgat tcgacagcta
    24481 atggaagagt gtggcgtaac ctgtcccctc tttacatcag atggtccatg gcgagctact
    24541 ctgaaagctg gaaccttaat tgaagaggac ctctttgtaa caggaaactt tggttctaag
    24601 gcaccttaca acttttcgca gatgcaggaa ttctttgatg aacatggtaa gaaatggcca
    24661 ctcatgtgta tggagttctg ggatggttgg ttcaatcgct ggaaagaacc gattatcaca
    24721 cgggatccta aggaattggc agatgcagtt cgagaggttt tggaacaagg ctctatcaat
    24781 ctttacatgt tccacggtgg tacaaacttt ggtttcatga atggttgctc agctcgagga
    24841 actttggacc tgccacaagt tacgtcttat gattacgatg cccttctgga tgaagaagga
    24901 aatccaactg ctaaatatct tgcagtcaag aagatgatgg caacacattt ttcagagtat
    24961 ccgcagttgg aaccactcta caaagagagt atggagttgg atgctattcc actagttgaa
    25021 aaagtttctt tgtttgaaac cttagatagc ttgtcaagtc ctgtagaaag tctctatcct
    25081 caaaagatgg aggagctggg acaaagttat ggctacctac tttatcgaac agaaacaaac
    25141 tgggatgcag aagaagaaag acttcgtatc attgatggtc gagatagggc ccagctgtat
    25201 gtcgatggtc agtgggttaa aactcaatat cagacagaga ttggggaaga tattttttat
    25261 caaggtaaaa agaaagggct atctaggtta gatatcttga tagaaaatat ggggcgtgtc
    25321 aactatgggc ataagttctt agcggatacg caacgtaagg gaattcggac aggggtctgt
    25381 aaggatctgc atttcttact aaactggaaa cactatccac tcccactaga caatcctgag
    25441 aaaattgatt tttcaaaagg atggactcaa ggacaaccag ccttttacgc ttatgacttt
    25501 acagtcgaag agccaaaaga tacttaccta gacttgtctg agtttggtaa gggagttgcc
    25561 tttgtcaatg ggcagaatct aggacgtttt tggaacgttg gcccaactct ctcactttat
    25621 atccctcata gctatctcaa ggaaggtgcc aaccgtatca ttatctttga aacagaaggt
    25681 caatataaag aagagattca tttaactcgt aaacctacac taaaacatat aaagggggaa
    25741 aacttatgac aattgtagga tgccgtattg atggacgttt gatccacgga caagtagcca
    25801 atctttgggc tggaaaacta aatgtttcac gcattatggt tgtagacgac gaagttgtca
    25861 acaacgatat tgaaaagagt ggtttgaaac ttgcgacacc accaggtgtg aaattgagta
    25921 ttttgccagt tgagaaagct gcagccaata ttcttgctgg taaatacgat agccaacgtc
    25981 tctttatcgt ggctcgtaaa ccagaccgct tccttggttt ggtagaagca ggtgtaccac
    26041 ttgaaaccct taatgttggg aatatgtctc aaacaccaga aactcgttct attacacgtt
    26101 ctatcaacgt agtagacaag gatgtggaag acttccacaa actggcagaa aaaggtgtta
    26161 aacttactgc tcagatggtt ccaaatgatc caatttcaga ctttttgagc ttattaaaat
    26221 aggaaaaaaa tttttaggag gtcattgtta tgatacaatg gtggcaaatt ttacttctca
    26281 ctttgtactc agcttatcaa atctgtgatg agttgacgat cgtttcatct gcaggttccc
    26341 ctgtatttgc tggtttcatt actggtttaa tcatgggaga tgtgactact ggtttactta
    26401 tcggtggtaa cttgcaactg ttcgttcttg gggttggtac cttcggtggt gcttctcgta
    26461 tcgacgcaac ttctggtgcg gttcttgcga cagccttctc tgtttcacaa ggaattgatg
    26521 caccgcttgc aattactaca atcgctgtac cagtagcagc tctcttgact tacttcgacg
    26581 ttcttggtcg tatgactact accttcttcg ctcaccgtgt ggatgctgca atcgaacgct
    26641 ttgactataa aggtattgaa cgcaactact tgcttggtgc gattccgtgg gctctatctc
    26701 gtgcccttcc agtcttcttt gcccttgctt ttggtggtgc ctttgtacaa tcagtagtag
    26761 acttcgttga agcctacaaa tgggttgcag atggcttgac acttgcagga cgtatgcttc
    26821 caggtcttgg atttgcaatc ttgcttcgtt accttccagt taaacgtaac cttcactacc
    26881 ttgctatggg atttggtttg acagctatgt tgactgttct ttactcatat gtaacaggtc
    26941 ttggtggcgc tgttgctggt atcgtaggta ctcttcctgc tgaagttgct gaaaaaattg
    27001 gtttcgtgaa caacttcaaa ggtttgtcta tgattggtat ttctatcgta ggtattttcc
    27061 ttgcagtgct tcacttcaaa aatagccaaa aagtagctgt agcagcacct tctacaccat
    27121 cagaaagtgg ggaaatcgaa gatgacgaat tctaattaca aacttacaaa agaagatttt
    27181 aatcaaatca acaaacgtag cttgtttact ttccaattag gttggaacta cgaacgtatg
    27241 caagcttctg gttaccttta catgatcttg cctcagttgc gtaaaatgta tggtgatgga
    27301 actcctgaat tgaaagaaat gatgaaagtt catactcaat tcttcaatac ttcaccattc
    27361 ttccatacca ttatcgctgg ttttgacctt gccatggaag aaaaagatgg tgtaggttca
    27421 aaagacgctg ttaacggtat caagacaggt ttgatgggac cattcgctcc tcttggggat
    27481 acaatctttg gttcacttgt acctgctatc atggggtcag tcgcagcaac tatggctatc
    27541 gctggccaac cttgggggat cttcctttgg attgcagttg cagtagcgta tgacatcttc
    27601 cgttggaaac agttggaatt tgcttacaaa gaaggggtta accttatcaa caacatgcaa
    27661 agtaccttga cagctttgat tgacgctgca tctgtacttg gtgtcttcat gatgggtgct
    27721 cttgtagcaa cagtgattaa ctttgaaatt tcttacaagt tgccaatcgg tgaaaagatg
    27781 attgatttcc aagacatctt gaaccaaatc ttcccacgtt tgcttccagc aatctttact
    27841 gcctttatct tctggttgct tggtaaaaaa ggtatgaact ctactaaagc tatcggtatt
    27901 attatcgtac ttgctttggc tctttctgcc cttggtcact ttgcacttgg aatgtaattc
    27961 cttatgacta aatcattaat tttggtgagc catggtcgct tctgtgagga gcttagaggt
    28021 agcacagaaa tgattatggg cccacaagac aacatttaca cagtagctct tcttccagaa
    28081 gatggcccag aagaatttac tgctaaattt gaagctgtta ttgaaggatt ggatgatttc
    28141 ctagtctttg cggatcttct cggtgggaca ccttgtaatg tggtgagtcg cttgatcatg
    28201 gaaggtcgtg atattgacct ttacgcaggg atgaatcttc caatggtgat tgaatttatc
    28261 aatgcgagcc ttacaggcgc agatgcggac tacaagagcc gtgctgcaga aagcattgtg
    28321 aaagttaatg acctgttagc gggcttcgat gatgacgaag atgaataata ctcttcgaaa
    28381 atctcttcaa actacgtcaa cgtc
//
LOCUS       DAVBCN010000022        27266 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVBCN010000022 DAVBCN010000000
VERSION     DAVBCN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27266
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.022"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            219..1901
                     /locus_tag="VQ497_000861"
     CDS             219..1901
                     /locus_tag="VQ497_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2517200.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNMSIS
                     KGQVQLQNELVKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHRKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            1886..2716
                     /locus_tag="VQ497_000862"
     CDS             1886..2716
                     /locus_tag="VQ497_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2517201.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVASLLLSLQNQGRFYNPWK
                     "
     gene            2909..3412
                     /locus_tag="VQ497_000863"
     CDS             2909..3412
                     /locus_tag="VQ497_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HET2517202.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     regulatory      3466..3700
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3865..4428
                     /locus_tag="VQ497_000864"
     CDS             3865..4428
                     /locus_tag="VQ497_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2517203.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTIGLTVAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYILLKPTLKHYER"
     gene            4442..5068
                     /locus_tag="VQ497_000865"
     CDS             4442..5068
                     /locus_tag="VQ497_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET2517204.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRSLTRLIDIPVIITWVVIIAFVFYRKRWKIESFFMLGNLTLAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGALFIEFPFYDKLRFQWRFKGKQK"
     gene            5180..6052
                     /locus_tag="VQ497_000866"
     CDS             5180..6052
                     /locus_tag="VQ497_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000525616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2517205.1"
                     /translation="MGMLLATRLKNKRKELKLSQKELAEGICEQGQISRMEQGKYSPG
                     SELLFQLSKRLKVSMDYFFEDTEVSSLENIDKFKELSKKFLDEREYESLHYIYELEKS
                     KRARLSAKDQIYLDWIDTLVQFCYLNHQTGAVAKLEKILLSLKKSDFYYLQLGNTLAN
                     FYFDMGQEDTYQELYQLLKENLQSLSVHHLEQLELMIKFRYNYCRYLWLNERTSEAIE
                     EITETIAICKRYNSSYRLADLYCLLGSISEGFAERDRILGFYQVSYQLYKHEENQRMM
                     LEIEKEMKLLESED"
     gene            6182..6319
                     /locus_tag="VQ497_000867"
     CDS             6182..6319
                     /locus_tag="VQ497_000867"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517206.1"
                     /translation="MKRSQVVKRCILIALLLLFLLNVLSGSLSVTTRGVDPRPAYGVS
                     L"
     gene            6743..7657
                     /locus_tag="VQ497_000868"
     CDS             6743..7657
                     /locus_tag="VQ497_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HET2517207.1"
                     /translation="MKLYQINDGIQIFESSNLEKIVIRNYRNGKKITIDHQPLLKKLL
                     LFLEVPKREEEILLYLPELSNKTLRSVLTNFMSLNIIHCQDTLIIHKKINILIIGLGT
                     TGGYLADGLCRSNINSKLFLLDKDRVDETNVGRQIYLRDDIGQYKVDVFKQKFSDKEI
                     KVFKIFINSKKDLEKICKREGIDLVIQCGDSPSPRELGKIVNTVCDTLQIPYIVNTGY
                     MSNVVPLPEFYYPNNRYNFNYKHQFSDEKLLFSQVLSKADYMVAIQSSFVMLMQIKNF
                     IFNEEPIYYGYRGYYNSVTLAWEVEKID"
     gene            7650..8663
                     /locus_tag="VQ497_000869"
     CDS             7650..8663
                     /locus_tag="VQ497_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517208.1"
                     /translation="MTKYLELDYSHSYILEGFRKNDAFIENHKECLDMLSDKIWRDNK
                     YMNTEKNISSPTRTILSRYTCNILSSLFIDISKNSIEKLIVTCENSDLNWIYNYYKSV
                     YNGVFCELRQLIQQYCKVKDKYEKWLFIKAIINQGIRQNEKEVVEFFLKQLKDNNQGI
                     FDYINSFSFYLLKFYSKDKSRIFLEEQLDIDDFLGSDKEDYARSLVFKNYASLLPDTS
                     ELKRNIMEKCLSQTPQDTDLWKEWFETYASQKEIRQKATEIFKHGYSDISLLKLVKIE
                     PDDTESLIRMIILCTSDLNSNIARYLISFLSDGRLKEFLELFVENFDFLNIIEGKTSE
                     INF"
     gene            8647..10239
                     /locus_tag="VQ497_000870"
     CDS             8647..10239
                     /locus_tag="VQ497_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2517209.1"
                     /translation="MRLIFRIIRKDIILYSILTFLLSIAMVSEAFLMQFIVDSIKFGE
                     KKYVLISIFIVIFLILQTLLYYYQQFLTAVLSKKSAYEYRKLIFKNIQQTSLRLVTGE
                     KNDSILASLTTQIDQIEYNYFYSVYWGGYLLCQLVVAIVMSLYLNPLMSVLTICLSLP
                     NLFVALLFKNSLEQKQEELINETNSSIAVTQDLIEGITDWKIANREPNIFRVFESKTL
                     KLLEKQVKVEKFQYIVVSLNQLFSNVLYFGSWIVSGFLIIRGQLSLGSLIAFSQLLAR
                     ISYPVYASSDLLSKYISGKKIVEILSKEFIFSDESRNYPDEFNVISFEHYFTNIDEKD
                     MPFDKEFYKNKKYLLKGKSGSGKTTILKSILREYDDYSGIVKINGVDVRDIKESVLFD
                     NIAYVSQKPHLFSASLRDNLTLFSEDFTDNEIVEVLKFVELSKWANKDCLDMMLSNDD
                     IKLSGGEVKRVSLARALLMNKNILLLDEFSAGIDYETLLKIEKKLIDLDKMIIYVTHV
                     DIDRSGKQFDDVIDLDCLFISS"
     gene            10431..11334
                     /locus_tag="VQ497_000871"
                     /pseudo
     CDS             10431..11334
                     /locus_tag="VQ497_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091364.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11352..11489
                     /locus_tag="VQ497_000872"
                     /pseudo
     CDS             11352..11489
                     /locus_tag="VQ497_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
     gene            11517..11858
                     /locus_tag="VQ497_000873"
     CDS             11517..11858
                     /locus_tag="VQ497_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517210.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLKNHI
                     LPLWIGMVSFF"
     gene            11831..12142
                     /locus_tag="VQ497_000874"
                     /pseudo
     CDS             11831..12142
                     /locus_tag="VQ497_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            12270..12857
                     /locus_tag="VQ497_000875"
     CDS             12270..12857
                     /locus_tag="VQ497_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HET2517211.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            13247..14854
                     /locus_tag="VQ497_000876"
     CDS             13247..14854
                     /locus_tag="VQ497_000876"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET2517212.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(15413..17044)
                     /locus_tag="VQ497_000877"
     CDS             complement(15413..17044)
                     /locus_tag="VQ497_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HET2517213.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGITITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            17337..22316
                     /locus_tag="VQ497_000878"
     CDS             17337..22316
                     /locus_tag="VQ497_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HET2517214.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVAIASETASPASNEAATTE
                     TAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEK
                     EVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDAITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKPRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KAAARSLGNNKVQVGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQK
                     TGRQTLTLRYQGHEVNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASSEVKEA
                     FLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEVQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEELTTAKQSLKDLIALLKEDKPAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESA
                     EKPQPAKPAPQNTVLPKPTYQPTSDQQKAPKLEVQEEKVAFHRQEHENTEMLVGEQRV
                     IIQGRDGLLRHVFEVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQ
                     NTAVKSEEASKQLPNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            22406..23602
                     /locus_tag="VQ497_000879"
     CDS             22406..23602
                     /locus_tag="VQ497_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET2517215.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            23738..24265
                     /locus_tag="VQ497_000880"
     CDS             23738..24265
                     /locus_tag="VQ497_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET2517216.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRVRLFLEKKEK"
     gene            24342..24698
                     /gene="glnR"
                     /locus_tag="VQ497_000881"
     CDS             24342..24698
                     /gene="glnR"
                     /locus_tag="VQ497_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HET2517217.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRCALHNEL
                     LQQGRFASVQSPFGRG"
     gene            24735..26081
                     /gene="glnA"
                     /locus_tag="VQ497_000882"
     CDS             24735..26081
                     /gene="glnA"
                     /locus_tag="VQ497_000882"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET2517218.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(26177..26296)
                     /locus_tag="VQ497_000883"
     CDS             complement(26177..26296)
                     /locus_tag="VQ497_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HET2517219.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            26784..27266
                     /locus_tag="VQ497_000884"
                     /pseudo
     CDS             26784..27266
                     /locus_tag="VQ497_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         8590 a         4569 c         5790 g         8317 t
ORIGIN      
        1 tacggcaagg cgaaactgac gtggtttgaa gagattttcg aagagtataa aatgatgggg
       61 aaggctgggc aactagtctt tttcgatgtt tatagattag ttaggcaagt aagctaggat
      121 aggaatcttt ttggtgctaa gattttaaga gaagagactg caagaagggc ttgatttatg
      181 ataaaataga agtctaagaa gcgaatgaag agagtaagat gaaagaagct ataattgagt
      241 ggaaggattt ctctttccgg tatgaaacac aacaagaacc gaccttgcaa gggatagact
      301 tgaccattta caagggagag aaagtcttaa ttgttggacc atctggatca ggtaaatcta
      361 ccttgggtca gtgtttgaat gggattattc ccaatattta caagggtcag acatatggag
      421 aatttttgat aaagggtcaa acagcctttg atatgagcat ctatgataag tctcatctgg
      481 ttagcacagt tttgcaggat acagatgggc agtttattgg cttgtctgtg gcagaagatt
      541 tggcgtttgc tctggaaaat gatgtgacag ccctagatga gatgaaaggt cgtgtttata
      601 aatgggctga aaagctggac cttcttcctt tactggatca gcgtcctcag gatttgtcag
      661 gtggacaaaa gcagcgagtc agtctggctg gtgtcttgat tgatgaaagt ccgattctct
      721 tgtttgatga gccactcgcc aatctagatc ccaagtcagg tcaggatatt atcgaattga
      781 ttgaccagat tcataaggaa gaggggacga cgactcttat tatcgagcac cgtttggagg
      841 atgttctgca tcgccctgtg gatcggattg tcttgataaa cgatggtcgt atccttttta
      901 atgggagccc tgaccagttg cttgcgactg atttattgac tcaaaatgga attcgagaac
      961 ccctttatct aaccactctc cgtcaattag gtgtggactt agtcaaggaa gaacagttag
     1021 cgaatctgga taacatgtct atctcaaaag gtcaggttca gctgcagaat gaactggtaa
     1081 aagaaactcc agaattgcag tcactcttta aattagagga cgtgtctttc tcttatgatg
     1141 atagaccgat tttaaaatcc ctacatttag atattaaaaa gggtgaaaag attgctattg
     1201 tcggaaaaaa tggagcaggg aaatcaactc tagccaaggc tataagtagc tttattcaga
     1261 cggaaggacg ctatctttgg gaaaaacagg atataaaagg cgattctgtt gcagagcggg
     1321 cggaacgagt aggctatgtg ctacaaaatc ctaatcaaat gatttcaacc aatatgattt
     1381 ttgatgaggt ggctctaggg ctccgtttgc gaggtgtgga tgagaaggaa attgaaacga
     1441 gagtctatga aaccttgaaa atctgtggac tttatgaatt ccgtaattgg cctatttctg
     1501 ccctgtcatt tggtcagaaa aaacgtgtca ccattgcttc aattttggtc ttaggagctg
     1561 aaattattct cctagatgaa ccgactgcag gtcaagatca gaagaactat actgagatta
     1621 tggaatttct cgaagagtta catcgaaaag ggcataccat tgtcatgatt acccatgata
     1681 tgcaattgat gctggattat tcagaccgag tccttgtcat ggtggatgga gaattgattg
     1741 ccgatactgt tccagccagt ctgttgagcg atcctgagct gttagtaaaa gccaatctaa
     1801 aagaaacctc catctttaac ttggctaaga aactagatgt ggatccactg gatttaacgg
     1861 cattttacaa agaaaggaga gagggatgca agctaaatta atcggttacc agcatagaga
     1921 tactgtgatt catcgcttgt caggagctgg gaaacttctc tttttcattc tggtatcatt
     1981 ggcggccatg attagctatg ataccagaat gctcttgctg attgccatct tttcagtctt
     2041 tctcctctat ttatcagaaa ttcgctttaa agatgtttcc tttgtagccg tttttgcgac
     2101 ggtatttgcc gttttaaacg ttttgatggt ctatctcttt tctcccgagt atggggttgg
     2161 actttatgga gagagaagtg tgatttggca gggaatcggt gtctacactc taaccagcca
     2221 ggagctcttt tatctgctaa atttggccat taagtatctt tgcaccattc ctctggctat
     2281 tatctttttg atgacaaccc atcctagtca gtttgcttcc agtttaaatc aaattggtgt
     2341 gccctataag attgcttatt ctgtcagcct gaccttgcgc tatattccag atttgcagga
     2401 agaattcttt actatcaaga tgtctcagga ggcgcgtggg atggaattat ccaagaaagc
     2461 ttctcttatg caacgaatca aaggcaatct gctcattatt acgcccttga tttttagctc
     2521 gctagaacgc attgatacca ttgcgactgc catggagctt cgccgctttg ggaaagaaaa
     2581 aaaacgcaca tggtatagtt atcaggcctt gaaaaaagga gactatatta ccttgctctt
     2641 ggcaaccttg tttttagtag ctagtttact acttagcttg cagaatcagg gacgatttta
     2701 caacccttgg aaatagcttg aaaaaattga aaaaatcaag tcgtttctat tgacaatgat
     2761 tctgaaagtg ttatactaag aaagtagttt cgctgattta cttcaaacct gttgtgaggt
     2821 aagttaacga tgccttaacc acgctgtttg ctgagcttga ctccgggcag tgtggctatt
     2881 tttttgcaat ggtgaaagga agcaagtcat gacaaatcac attgtattat ttgaacctca
     2941 aattccacaa aatacgggca atatcgcgcg tacttgcgct gcgaccaatt ctcccctcca
     3001 catcatcaag cctatgggct ttcctattga tgaccgcaag atgaagcggg ctggattgga
     3061 ttactgggat aagctagaga tttattttta cgagagtttg gaagatttca tatctcagat
     3121 gaagggaaaa ctctatctga tttcgaaatt cgcggaaaaa gtctattctg aggtggattt
     3181 atcgactgac gaggaccatt attttctctt tggacgtgaa gacaagggct tacctgaaga
     3241 ttttatgcga gaacatcctg agaaggctct ccgtattccc atgaatgatg aacatgtccg
     3301 cagtctcaat gtgtctaata ccgtctgcat gattgtctat gaggctcttc gccagcagaa
     3361 ctttgcaggt cttgaactcg tccataccta tgaagtggat aaattgaaat aaaaaatgaa
     3421 aatgaacaaa atgcttgcgc ttgcaagcgt tttttgttat gataaaagag tcttcagggc
     3481 agggtgtgat tcccgaccgg cggtgacttt aactaggaaa tgatcttttc cttttatact
     3541 ttgttgacaa gctttgccta accagaagtt atgcctacag cttgtcgcct agtctaaaag
     3601 aaaaatctct atttccttct ctttaaagaa gtccgcgagc gcaagctgat gtggtgagat
     3661 tccacaaccg acagtatagt ctggatggga gaagacgaaa gaatagcttt gtctgttttg
     3721 atagatttat agccggattg taaccacttg ctttgttttt ctttaataga gtaggaggga
     3781 acttttggga tataaaaagt gagaatagat agaggaatcc tttccaactt cttctgattt
     3841 tatagaaaat tggaggaacc tgttatgaca aacacacgtc gactttcgac cattgcaatt
     3901 ttatcagcca tctcatttgt gctgatgtac tttgactttc cgcttttacc agcggcatct
     3961 ttcctcaaga tcgaatttag tatcttgcca gtccttgtgg gtctggtggt catggatttg
     4021 cctgctgctc taggagtgct cttgcttcgc tcactcttga aattgcttct taacagccaa
     4081 ggagtgaata cttacattgg tttgccgatg aatatcgtag ctttgggagt ttttgtcatc
     4141 gtatttgctt tgatttggaa aaaggaacgg acaacccttc gtttcctact aggctctcta
     4201 gctgggacta ttggtttaac cgtggctatg ttagttctca actatgttta tgctgttcct
     4261 ttgtacgcta agtttgctaa ctttgatatt ggaaaaattt tgggactttc caactaccta
     4321 atgaccatgg tattaccttt taacttgatt gaaggtgtaa tcttttccgt ttcattctgg
     4381 ttgttgtaca ttctcttgaa accaacctta aaacattatg aaagataaac aaacattttt
     4441 aatgaagggc agttttgccc ttttactttt cgttattctt ggctatatgg tcaaatttta
     4501 ccctgaaatg ttggtcaatt ttgaccaatc gattcagact gccattcgag gagacttacc
     4561 agattatttg actattcttt ttcgatccct cacacgtctg attgatattc cagtgattat
     4621 cacttgggtt gtcattatag cttttgtctt ttatcgtaag cgatggaaga tagaaagttt
     4681 cttcatgctg ggaaatctga ctttggcagg tcttttaatc gtgaccttta aaaatatcta
     4741 ccagcgccca cgaccagcta tcttacatct ggtggaggag aagggatttt ccttcccaag
     4801 tgggcattct ctggctgtaa cgctgatggt agggactttg attgttattc ttagtcagcg
     4861 gattaaagat caggtctgga gaaaaatcgt acaaatcgtc cttggcctct acctagtcag
     4921 tgtgctggta tcaagagtct atctgggagt tcattaccca tcagatgttc ttgccagtct
     4981 ctgtgtgggc ttgggagccc tgtttatcga gtttcccttt tatgataagc tccgcttcca
     5041 atggcgattt aaaggcaaac agaaatgatg gaaaagataa aaaattgtac aaatacaaat
     5101 agaaccatat gaaatatata cttgaaacat gttttggagt ttgatatatt ttaataaata
     5161 aagcaaggag gaagaaagtg tgggtatgtt attagcgaca cgcttaaaaa ataaaagaaa
     5221 ggaattaaaa ttatctcaaa aagaattagc tgaggggatt tgtgagcaag ggcagattag
     5281 ccgtatggag caaggtaaat actcacctgg gtcagaatta ctctttcaac tatctaagcg
     5341 attgaaggtg agcatggatt atttctttga agatactgag gtttctagtt tagaaaatat
     5401 tgataagttt aaagaattat ctaagaaatt tttggatgag agggaatatg aatctctcca
     5461 ttacatctat gaactggaaa aatctaaacg tgctcgtcta tctgctaaag accagattta
     5521 tttggattgg attgatacat tagttcaatt ctgttattta aatcatcaaa caggggcagt
     5581 tgcgaaactg gaaaaaatac tgttatctct gaaaaaatct gatttttact atctacaact
     5641 gggaaatacc ttggctaatt tttattttga tatggggcag gaagacacat atcaggaact
     5701 ctatcaactt ttaaaagaga acctccagtc actatctgtt catcatttgg aacagttaga
     5761 attgatgatt aaattccgat ataactattg tagatatttg tggttgaatg aacgaacatc
     5821 tgaagctata gaagaaatta cagaaacgat agctatttgt aaaagatata atagtagtta
     5881 tcgattagca gatttgtatt gtttattggg aagtataagc gagggctttg ctgaaaggga
     5941 taggatacta ggtttttatc aagtatctta ccagctttac aagcacgaag agaaccagag
     6001 aatgatgcta gaaattgaga aggaaatgaa attattggaa agtgaagatt aagcaaaaag
     6061 atttgtattc ttagataact ttaatacata ttataaaaaa aataaaaaaa tatcataaaa
     6121 attattgaca aaccaaaatt gatgtgttag aatatcacca atagttaagg aggtttagaa
     6181 gatgaaaaga tctcaagttg tcaaacgatg tatattaatt gctttattgc ttctatttct
     6241 acttaatgtg ttaagtggtt ctttatctgt aacaactaga ggagttgatc caagaccagc
     6301 atatggagtt tcgctttaaa gttgtgcata gttcttcttt tatagacgga gagtttttct
     6361 caatagtaag tgattcaaat tttgatggat gattttccat taaattaagt gattgcgaaa
     6421 actttttgaa atacattctc tttggatatt gaaagtttgt tttggatttt tttatgaagc
     6481 ttatcttagc tgttagattg gggttttatg ggatttgtcc tcaccgggaa aaaatcttaa
     6541 atctttgaag aaagaggttt ttatcttatg gttagagata catcagaaga aattgaagtt
     6601 attgacttgt cttctatgac cgtttcctac tcatctgtta tgctaggaaa ctcttggatg
     6661 acgtggaatt gatttattaa gatagattat ttggttttta ccaaaaatct gtctttttaa
     6721 tttttaaagg actaatggtg atatgaaact atatcaaata aatgatggga tacaaatttt
     6781 tgaaagtagt aatcttgaaa aaatagtaat tagaaattat aggaatggta agaaaataac
     6841 tatcgatcac caaccattat taaaaaaatt actactattc cttgaagttc ctaaacgtga
     6901 agaagaaatt ctattatatc ttccagaatt atcaaataaa acattacgaa gtgttttgac
     6961 gaattttatg agtttaaata taattcattg tcaagataca ttaataattc acaaaaaaat
     7021 aaacatattg ataataggtt tagggactac gggtggatat ctggcagatg ggttatgtcg
     7081 tagtaatatt aattcgaagc tatttctttt agataaggat cgtgtagatg agactaatgt
     7141 aggtagacag atttatttaa gagatgatat tggtcaatat aaagttgatg tatttaaaca
     7201 aaagtttagt gataaagaaa taaaggtatt caaaattttt attaatagta agaaagattt
     7261 ggaaaagatt tgcaaaaggg agggtatcga tttggttata caatgtggtg actccccttc
     7321 acctcgagaa ttaggaaaaa ttgtgaatac agtatgtgat actctccaga taccttatat
     7381 agttaacact ggctacatga gtaatgtagt tcctttgcca gaattttatt atccaaataa
     7441 tagatacaat tttaattata agcatcaatt ttcagatgaa aagttactgt tctcacaagt
     7501 tttgtctaaa gctgattata tggtggcaat tcaatcatca tttgtaatgt taatgcaaat
     7561 aaaaaatttt atatttaatg aagaacctat ttattatggt tataggggat actataattc
     7621 tgtaacatta gcatgggagg tggaaaaaat tgactaaata tttagaattg gattattctc
     7681 attcatatat tttggaaggt tttagaaaaa atgatgcgtt tattgaaaat cacaaagaat
     7741 gccttgatat gttatcagat aagatttggc gagataataa atatatgaat acagaaaaaa
     7801 acatatcatc accgacacga acaatcttat cacgctatac ttgtaatatt ttatcgtctt
     7861 tgtttattga tattagcaaa aatagcatag aaaaattgat agtgacatgt gaaaatagcg
     7921 atcttaattg gatttataat tattataaat cggtttacaa tggagtattt tgtgaattgc
     7981 gacaacttat acaacagtac tgtaaggtga aggataagta tgaaaagtgg ctttttataa
     8041 aagctattat aaatcaaggt attcgtcaaa atgagaaaga agttgttgaa ttctttttaa
     8101 aacaattgaa ggataataat caaggtattt ttgattacat caatagtttt agtttttatc
     8161 tattaaagtt ttattcaaaa gacaaatctc gcattttttt agaagagcag ctagatatag
     8221 atgatttttt gggatcagat aaagaagatt atgctaggtc tttagttttt aagaattatg
     8281 cttctctatt gcctgatact tctgaattaa aaagaaatat tatggaaaaa tgtttaagtc
     8341 aaacacctca agatacagat ttatggaaag aatggtttga aacttatgcc agtcagaaag
     8401 agattagaca aaaagcaacg gaaatattta agcatggtta ttcagatatt tcgttactaa
     8461 aattagttaa aattgagcct gatgacactg agagtttaat cagaatgatt atcttatgta
     8521 ccagtgacct aaatagtaat attgcaaggt atttgatttc atttttgagc gatgggcgat
     8581 taaaagaatt tttggagtta tttgttgaaa attttgactt tttgaatatt attgagggaa
     8641 aaactagtga gattaatttt tagaataatt agaaaagata ttatattata ttctatatta
     8701 acatttttgc tatcaatagc gatggtaagc gaagcatttt tgatgcaatt tattgttgat
     8761 tcaattaaat ttggtgaaaa gaagtatgtt ttgatttcta tttttattgt aatttttttg
     8821 attcttcaga ctttattgta ttactatcaa caatttctta cagctgtatt aagtaaaaaa
     8881 tctgcatatg agtaccgtaa attaattttt aaaaatattc agcagacttc tcttaggctt
     8941 gttacaggtg aaaaaaatga tagtattcta gcttctttga caacgcaaat cgatcaaata
     9001 gaatataatt atttttattc tgtttattgg ggtggatatt tgctttgcca attagtggta
     9061 gctatagtta tgtcgcttta tctaaatcca ttgatgtctg tattaaccat ttgcttaagt
     9121 ctccctaatt tatttgtagc acttcttttt aaaaactcct tggaacaaaa gcaagaagag
     9181 ctaattaatg agacaaattc ctcaatagca gttactcaag atttaattga gggaattact
     9241 gattggaaaa ttgcaaatag agaacctaat atatttagag tgtttgaaag caaaacattg
     9301 aagcttttag agaagcaagt gaaggtagaa aaatttcaat acattgttgt tagtttaaat
     9361 caattgtttt caaatgtttt atattttggt agttggatag taagtggatt cttgattata
     9421 cgggggcaac tttcattagg gagtttgata gcgttctctc aattattagc aagaatttct
     9481 taccctgttt acgcttcttc ggatttattg tcaaaatata ttagcggtaa aaaaattgta
     9541 gaaattcttt ctaaagaatt tatattttca gacgaaagca gaaattatcc agatgagttt
     9601 aatgttattt catttgaaca ttattttaca aacattgacg aaaaggatat gccttttgat
     9661 aaagaatttt acaaaaataa aaaatatttg ttgaaaggga aaagtggtag tgggaaaaca
     9721 acaattttaa aatcgatttt acgagagtat gatgattata gtggaattgt aaaaattaat
     9781 ggggtagatg ttagggatat aaaagagagt gttctttttg acaatatagc ttatgtttcc
     9841 caaaaacctc acttgttcag cgcaagtctt agagacaacc tgacattatt ttctgaagac
     9901 ttcactgata atgagatagt tgaggtttta aaatttgttg aattatctaa atgggctaat
     9961 aaagattgct tggatatgat gctttcaaat gatgatataa aattatcagg aggggaggtc
    10021 aaaagggttt ctttagctcg agccttattg atgaataaga atattctgtt attggatgag
    10081 ttctctgctg ggattgatta tgaaacattg ttaaaaatag agaagaaact cattgatttg
    10141 gataagatga ttatttacgt gacgcatgtg gatatagata gatccggcaa acagtttgat
    10201 gatgtcattg atttggattg tctttttata tcctcgtgac atgagagttg agtcagttga
    10261 attattttgt cactctgttt agttcttgtt ggtagtgtca ggtaatcttt gttaattttt
    10321 caggaagtct attgaaatat cctcttggtt atatattacg aggagtaatt aatggatatt
    10381 ctcgagaatt caatacaaca atttgattct aaagttaagt atgtttcttt atttgcggaa
    10441 tcaatctttt atcagatacc atgagctatt ttgcaggtgc tatgatttct cctttgtatg
    10501 tgaggattat cggagatgat atgacagatg ctatgggggt tagacaggca actgctggtg
    10561 tagttaatat tctcagcaac cttatagggg gagttttgat tgggggtcat cagtcttcag
    10621 atgtttgtag gtttgaatgc tttgaccttt ctttttgcac tgttgggtgt ggtgtttatt
    10681 cgtacttctt tgagagatat tgaggaacag atggaggtta gtaaaagatt agccgcaaaa
    10741 tcttttggag agcatttcct ccattcaatg aagatattgt caagttttcc agccatcatc
    10801 aaattaatat tcattgcagc tatgaaccaa gtgattttaa gcattattat ccctttaacg
    10861 actttgatgt taatccatca tccatttatc ttctttgaca ctgggaaatc cattgcagca
    10921 ttgtcagtgg ttattttttc aggaacgatt gtgggaagtt ttttgagtgg aggtatccta
    10981 aaaaatattc ccataaaaat gattgtctac tttgaagaaa ttatgcatct ctgtattctc
    11041 ttttcttggt tgaccaatca tttcctcctt ttattggtag ttgtcttctt atgcgcagtt
    11101 gcagtaggaa tgatggggcc tcggattttc aaccttgtat tctcgatggt tccagaagaa
    11161 gtcatgggaa ccatccagtc agtgcttgga gtattcaatg ttgttgtacc aggaattctt
    11221 agcatgctca tcgttgcctt agcgagtgct acaaacctaa aaatcacagt gcttccactt
    11281 tttatttttg tattgactgc cttagcaatt gtgaaagcaa tgaaaataga gtaatagtag
    11341 tatgaactac tgtctctttt tgggtgggag ttcattaccc atcagacgtc cttgccagtc
    11401 tctgtgtggg cttgggagtt ttgtttatcg agtttccctt ctatgacaag ctccgcttcc
    11461 aatggcgatt taaaggcaaa cagaagtgag tatcgaattc ccttgaggag aaagaaatga
    11521 aagtcaatat agcagatctt catccgactc aactatattt atcagaaaag aagttgcaag
    11581 atattcagat gctttaccag tcggcagaaa caatccaagt cgatccaatc agtattcttg
    11641 cctttggaga ttgtttgctg attacagatg ggcatcacag ggcttatcag gctttattgg
    11701 caggtcggga tacgatttct gctgagtggg atagagatgg tggtgatgaa ctatatcatc
    11761 tctatgcgca agcttgcgag gaaagaaaga tttactctgt ttttgattta aaaaatcata
    11821 tcttacctct ttggatcggc atggtgagct ttttttgatt gaagtcaagg agaagggaga
    11881 atggtttcca attggggata ttacactatc tcaggataat ctccccattg tgattgggaa
    11941 ttccgcttac caacatcgag gacttggaaa aaagattcta agtgctttga ttgaattggc
    12001 tcgagtaaaa ggatggaaag aattgagagt caaggaaatc tacacctaca atcatgcttc
    12061 taggaggtgt ttcaagtcgc ttggatttgt ggaaaatgga gcaacagaaa aaggaaggag
    12121 ttttatattg gaattagtct aatcctgcca ctttctcccc cttaaccttt gactattccg
    12181 caaaattatc gtaaaataaa gagtaaatga taaaatgagg tcagagtctg ttcgctctga
    12241 cgatagtagt ataaatgagg agaaacgctt tggaattaga agtatttgct gggcaagaaa
    12301 aaagtgaact atctatgatt gaggtagcgc gtgctatatt agaacttcgt ggtcgcgatc
    12361 atgagatgca ttttagcgat cttgtaaacg aaattcaaaa ctaccttgga acatcaaaca
    12421 gcgatatccg cgaagctttg cctctgttct acacagagtt gaactttgac ggtagcttca
    12481 tctcacttgg ggacaacaaa tggggtcttc gttcatggta tggtgtggac gaaatcgacg
    12541 aagaaatcat cgctcttgaa gaaaatgacg acgatgaagt agcaccaaaa gctaagaaaa
    12601 aacgtgtcaa tgcctttatg gatggtgatt cagatgccat tgactacaat gcagatgatc
    12661 cagaagacga agatgcatac gaagcagatc cagctctttc atacgatgat gaaaatccag
    12721 atgatgaaaa aaatgaagtg gaagcttatg atgcagaaat caacgaaatc gctccagatg
    12781 acttgggaga agatgtggat ctcaacgaag acgacgacga gttttcagat gacgacgctg
    12841 aaaccagcga ggaataagag ttagctattg acaattatcc tatttttagg tatcatattg
    12901 tttgggcacc tcttttagag gtcggggctc cctagttctt agggagctat ttttgttttt
    12961 tcaagaagtt atcttcttgt attttatact caatgaaaat caaagagcaa gctaggaaac
    13021 tggccgtagg ctgctcaaaa cactgttttg aggttgtaga taagactgac aaagtcagga
    13081 acacatatct acggcaaggc gacgttgacg cggtttgaag agattttcga agagtattag
    13141 ttgtgaatct ggtgcagtcg tcccagatta ttcttattag tagggtcttg ttttctatat
    13201 cccctcgtag ttaacaagac cttgagcatt ttagaaagag gaatctatgt ctacgaaata
    13261 tatttttgta actggtggtg tggtatcgtc tattgggaaa gggattgtgg cagcgagtct
    13321 aggccgtctc ttgaaaaatc gtggtctcaa agtaaccatt caaaagtttg acccttatat
    13381 caatattgat ccgggaacca tgagtcctta ccagcacggg gaagtttttg tgacagatga
    13441 cggagctgag acagatttgg acttgggtca ctatgaacgt ttcatcgata tcaatctcaa
    13501 caaatattcc aacgtgacaa ctgggaaaat ttacagtgaa gttcttcgta aagaacgccg
    13561 tggagaatac cttggggcaa ctgttcaagt cattcctcat atcacagatg ctttgaaaga
    13621 aaaaatcaag cgtgccgctc taacgaccga ctctgatgtc attatcacag aggttggtgg
    13681 aacagtagga gatatcgagt ccttgccatt cctagaggct cttcgtcaga tgaaggcaga
    13741 tgtgggtgcg gataatgtca tgtatatcca tacaaccttg cttccttacc tcaaggctgc
    13801 tggtgaaatg aaaaccaaac caacccaaca ctctgtcaaa gaattgcgtg gcttgggaat
    13861 ccaaccaaat atgttggtta ttcgtacaga agagccagct ggtcaaggaa ttaaaaataa
    13921 actggcccag ttctgtgatg tggcaccaga agccgttatc gaatcgttgg atgttgaaca
    13981 cctttaccaa attccactga acttgcaggc acaagggatg gaccaaattg tttgtgatca
    14041 tttgaaatta gacgcaccag cagcggatat gacagaatgg tcagccatgg tggacaaggt
    14101 catgaacctc aagaaacaag ttaagatttc ccttgttggt aagtatgtgg agttgcaaga
    14161 tgcctatatc tcagtggtcg aagccttgaa acactctggc tatgtcaatg atgtagaagt
    14221 taaaatcaat tgggtcaatg ccaatgatgt gacagcagag aatgtagcag aactcttgtc
    14281 tgatgcggac gggatcatcg taccaggtgg ttttggtcaa cgtggtacag aagggaaaat
    14341 ccaagccatc cgctatgcgc gtgaaaatga tgttccaatg ttgggagtct gcttgggaat
    14401 gcagttgaca tgtatcgagt ttgctcgtca cgttttaggt cttgaaggtg ccaattctgc
    14461 agagcttgca ccagaaacaa aataccctat cattgatatc atgcgtgatc agattgatat
    14521 tgaggatatg ggtggaaccc ttcgtttggg actttatccg tctaagttga aacgtggctc
    14581 taaggctgct gctgcttatc acaatcaaga agtggtgcaa cgccgtcacc gtcaccgtta
    14641 tgagtttaat aatgccttcc gtgagcagtt tgaggcagca ggttttgtct tttcaggagt
    14701 ttctccagac aatcgtttgg tagaaatcgt ggaaattcct gaaaataaat tctttgtagc
    14761 ttgtcagtat caccctgaac tgtcaagccg tccaaaccga ccagaagaac tctacactgc
    14821 ctttgttact gcagcggttg agaacagcaa ttagcaaaat cagaaccttt gagaaaaatc
    14881 tcagaggttt tttgcatacg atgatattgc agtatatctg aggtaggggg cctctgtatg
    14941 tacctgctac cgttgaaatc aatagcgact ccctcttgcc ctgtgctagt gaatggattt
    15001 atcagtatat tgaaatgaaa taaaatttga acaaattaat tcggaaagcc aaatcaattt
    15061 ctagcaaagt tttaggaact ggattgtata gtgaattgaa ataagatgtg aacatctcta
    15121 tcagaaaagt caaattaatt tatagaaata ttttagcagt caagatgtac tgttatagat
    15181 tcaatacatt atactttttt aatttaatcc actatagtaa aatgaaataa gaacaggaca
    15241 aatcgatcag gacagtcaaa tcgatttcta acaatgtttt agaaatagag gtgtactatt
    15301 ctagtttcaa tatactatat atctgcagaa tactctttca gtttcattca gcctagtctt
    15361 gcttaggaaa aagtaaaaag ccatccaatt atgatggata gcttcttggt tcttagattt
    15421 gttctgcaaa aaccttttca gcaaggttca tagcgtggtc tgatacacga gtgtagtgtg
    15481 agatgatgtc gataaagttg accccagctt gtgttgaaca ttcgcctttg ttgaggcgtt
    15541 tgatgtgggt ttttctgaga acacgttcta tcttattgat tgcttcatga cgttctacaa
    15601 gactgcgtgc tttttcaata tcattgtttt ccacactatc cagagcatct ttgataaagt
    15661 cactagtttg gcggtaaact tcctctaatt ctttcaaggc ggcatcagaa aattcaacat
    15721 ttttccgttg aagatagtca gtcagattga gtagagcctc cgtgtggtct ccaatccgtt
    15781 ccaaatcacg ggaggaatca aggatattgg taagcacttc actttctttt tggctgagag
    15841 cttcgcttga aagggcaatg agataacgtg ttaattgctc atcgatggtg ttaattgctt
    15901 cttcggtttt atgccctttt tcagcaactt tttcatccaa gtcaatgatg tacttatagg
    15961 aaaggtcaaa ggctttagca gcgtagtttc ctaagtgcaa gagctctttc ttagcatttc
    16021 ctagagcgat agatggggcc tgtttgatga aatgttcatc aagatataag ggttcgtatt
    16081 tgacaacctc gtcctctcca ggaataatct tggttacaaa gtaagccaga gctccgataa
    16141 atggaaattg gacaatggtg ttggtaatat taaaggttcc gtgagcaaag gcgatggtca
    16201 tttccggtgc tagatttagc gtagcttcaa accaatgaat caggacagta aaaggaacta
    16261 gaaaaataac gcagacgact gttccgataa cgttgaaggc aacatgagct cctgctaccc
    16321 gtttagctgc aatattagcc cctaaagagg caatgatggc tgtaatggtt atcccgatat
    16381 tgtcaccaaa tagaactggc aaagctccct gtaggtcaat tagattgccg gcgtagaggt
    16441 tttgtaaaat cccaatggta gccgaagaag cttgaattag caaggtcaag ccggtaccga
    16501 caaagacacc caaaacagga ttcttactta gctcaatcat atagtcctta aagacctgta
    16561 aatccttgag tggagccatt gcgccgctca tgagattgag ggcaaaaaag ataccaccga
    16621 caccaaagag gatgcgtccg atattattga ctgtccgatt ttttgtaaaa aagagacaga
    16681 cggcaccgat aaagagcata ggtagggcat agttacctaa tttaaaaccg atgagaaagg
    16741 atgtgacagt tgtcccaata ttagcaccca tgacaatccc gatagcctga cgtaaggtta
    16801 agagaccggc actgaccagg ccgactgtga taactgttac accagaacta gactgaatta
    16861 gagcagtcat cccaatacca accagaactc caaagaaagg attactagta tatttgtcaa
    16921 tgtaaaaacg aaggcgatct ccagcagctt gttgcaaacc gtctcccatg gtcttgatgc
    16981 tatataagaa tagccccaga ccacctaaaa agtgaaataa aatttcctgc caattaatgg
    17041 acatttcttt ttcctccgaa aaataatcgc ggaatatctt ctattctatt ttaaaggata
    17101 aaagtaaatc taacaagtgt taatgtcaag tttgtaaaag aaaattataa ttgataagtg
    17161 tattattttt aagagttttt aaccttattt tagatttgtg caaacagtta ttgttttttg
    17221 tctaaaaatg ttttaggata aaaatgtgat tatcatggtt ctagagtttg ataaaaaact
    17281 acagaatata aagcgctttc ttgaaaacaa caaaatcaat cttttaggag gagaaaatga
    17341 agaatccatt ttttgaaaga cgttgtcgtt acagtattcg taagttatca gtaggagcct
    17401 gctcgctgat gattggtgct gttttatttg ctggtccagc cttggctgaa gaaactgcag
    17461 ttcctgaaaa tagcggagct aatacagagc ttgtttcagg agagagtgag cattcgacca
    17521 atgaagctga taagcagaat gaaggggaac atgctagaga aaacaagcta gaaaaggcag
    17581 aaggagtagc gatagcatct gaaactgctt cgccagcaag caatgaagct gcaactactg
    17641 aaactgcaga agcagctagc gcagctaaac cagaggaaaa agcaagtgag gtggttgcag
    17701 aaacaccatc tgcagaagca aaacctaagt ctgacaagga aacagaagca aagcccgaag
    17761 caactaacca aggggatgag tctaaaccag cagcagaagc taataagact gaaaaagaag
    17821 tccagccaga tgtccctaaa aatacagaaa aaacattaaa accaaaggaa atcaaattta
    17881 attcatggga agaattgtta aaatgggaac caggtgctcg tgaagatgat gctattaacc
    17941 gcggatctgt tgtcctcgct tcacgtcgga caggtcattt agtcaatgaa aaagctagca
    18001 aggaagcaaa agttcaagcc ttatcaaaca ccaattctaa agcaaaagac catgcttctg
    18061 ttggtggaga agagttcaag gcctatgctt ttgactattg gcaatatcta gattcaatgg
    18121 tcttctggga aggtctcgta ccaactcctg acgttattga tgcaggtcac cgtaacgggg
    18181 ttcctgtata cggtacactc ttcttcaact ggtctaatag tattgcagat caagaaagat
    18241 ttgctgaagc tttgaagcaa gacgcagatg gtagcttccc aattgcccgt aaattggtag
    18301 acatggccaa gtattatggc tatgatggct atttcatcaa ccaagaaaca actggagatt
    18361 tggttaaacc tcttggagaa aagatgcgcc agtttatgct ctatagcaag gaatatgctg
    18421 ctaaggtaaa ccatccaatc aagtattctt ggtacgatgc catgacctat aactatggac
    18481 gttaccatca agatggtttg ggagaataca actaccaatt catgcaacca gaaggagata
    18541 aggttccggc agataacttc tttgctaact ttaactggga taaggctaaa aatgattaca
    18601 ctattgcaac tgccaactgg attggtcgta atccttatga tgtatttgca ggtttggaat
    18661 tgcaacaggg tggttcctac aagacaaagg ttaagtggaa tgacatttta gacgaaaatg
    18721 ggaaattgcg cctttctctt ggtttatttg ccccagatgc cattacaagt ttaggaaaaa
    18781 ctggtgaaga ttatcataaa aatgaagata tcttctttac aggttatcaa ggagacccta
    18841 ctggccaaaa accaggtgac aaagattggt atggtattgc taacctagtt gcggaccgta
    18901 cgccagcggt aggtaatact tttactactt cttttaatac aggtcatggt aaaaaatggt
    18961 tcgtagatgg taaggtttct aaggattctg agtggaatta tcgttcagta tcaggtgttc
    19021 ttccaacatg gcgctggtgg cagacttcaa caggggaaaa acttcgtgca gaatatgatt
    19081 ttacagatgc ctataatggc ggaaattccc ttaaattctc tggtgatgta gccggtaaga
    19141 cagatcagga tgtgagactt tattctacta agttagaagt aactgagaag accaaacttc
    19201 gtgttgccca caagggagga aaaggttcta aagtttatat ggcattctct acaactccag
    19261 actacaaatt cgatgatgca gatgcatgga aagagctaac cctttctgac aactggacaa
    19321 atgaagaatt tgatctcagc tcactagcag gtaaaaccat ctatgcagtc aaactatttt
    19381 tcgagcatga aggtgctgta aaagattatc agttcaacct cggacaatta actatctcgg
    19441 acaatcacca agagccacaa tcgccgacaa gcttttctgt agtgaaacaa tctcttaaaa
    19501 atgcccaaga agcggaagca gttgtgcaat ttaaaggcaa caaggatgca gatttctatg
    19561 aagtttatga aaaagatgga gacagctgga aattactaac tggctcatct tctacaacta
    19621 tttatctacc aaaagttagc cgctcagcaa gtgctcaggg tacaactcaa gaactgaagg
    19681 ttgtagcagt cggtaaaaat ggagttcgtt cagaagctgc aaccacaacc tttgattggg
    19741 gtatgactgt aaaagatacc agcctaccaa aaccactagc tgaaaatatc gttccaggtg
    19801 caacagttat tgatagtact ttccctaaga ctgaaggtgg agaaggtatt gaaggtatgt
    19861 tgaacggtac cattactagc ttgtcagata aatggtcttc agctcagttg agtggtagtg
    19921 tggatattcg tttgaccaag ccacgtaccg ttgttagatg ggtcatggat catgcaggag
    19981 ctggtggtga gtctgttaac gatggcttga tgaacactaa agactttgac ctttattata
    20041 aagatgcaga tggtgagtgg aagctagcta aggaagtccg tggtaacaaa gcacacgtga
    20101 cagatatcac tcttgataaa ccaatcactg ctcaagactg gcgcttgaat gttgtcactt
    20161 ctgacaatgg aactccatgg aaggctattc gtatctataa ctggaaaatg tatgaaaagc
    20221 ttgatactga gagtgtcaat attccgatgg ccaaggctgc agcccgttct ctaggcaata
    20281 acaaggtaca agttggcttt gcagatgtac cggctggagc aactattacc gtttatgata
    20341 atccaaattc tcaaactccg ctcgcaacct tgaagagcga agttggagga gacctagcaa
    20401 gtgcaccatt ggatttgaca aatcaatctg gtcttcttta ttatcgtacc cagttgccag
    20461 gcaaggaaat tagtaatgtc ctagcagttt ccgttccaaa agatgacaga agaatcaagt
    20521 cagtcagcct agaaacagga cctaagaaaa caagctacgc cgaaggggag gatttggacc
    20581 ttagaggtgg tgttcttcga gttcagtatg aaggaggaac tgaggacgaa ctcattcgcc
    20641 taactcacgc aggtgtatca gtatcaggtt ttgatacgca tcataaggga gaacagaatc
    20701 ttactctcca atatttggga caaccagtaa atgctaattt gtcagtgact gtcactggcc
    20761 aagatgaagc aagtccgaaa actattttgg gaattgaagt aagtcaggaa ccgaaaaaag
    20821 attacctagt tggtgatagc ttagacttgt ctgaaggacg ctttgcagtg gcttatagca
    20881 atgacaccat ggaagaacat tcctttactg atgagggagt tgaaatttct ggttacgatg
    20941 ctcaaaagac tggtcgtcaa accttgacgc ttcgttacca aggtcatgaa gtcaactttg
    21001 atgttttggt atctccaaaa gcagcattga acgatgagta cctcaaacaa aaattagcag
    21061 aagttgaagc tgctaagaac aaggtggtct ataactttgc ttcatcagaa gtaaaagaag
    21121 ccttcttgaa agcaattgaa gcggccgaac aagtgttgaa agaccatgaa actagcaccc
    21181 aagatcaagt caatgaccga cttaataaat tgacagaagc tcataaagct ctgaatggtc
    21241 aagagaaatt tacggaagaa aagacagagc ttgatcgctt aacaggtgag gttcaagaac
    21301 tcttggctgc caaaccaaac catccttcag gttctgccct agctccgctt cttgagaaaa
    21361 acaaggcctt ggttgaaaaa gtagatttga gtccagaaga gcttacaaca gcgaaacaga
    21421 gtctaaaaga tctgattgct ttattgaaag aagacaagcc agcagtcttt tctgatagta
    21481 aaacaggtgt tgaagtacac ttctcaaata aagagaagac tgtcatcaag ggtttgaaag
    21541 tagagcgtgt tcaagcaagt gctgaagaga agaaatactt tgctggagaa gatgctcatg
    21601 tctttgaaat agaaggtttg gatgaaaaag gtcaagatgt tgatctctct tatgcttcta
    21661 ttgtgaaaat cccaattgaa aaagataaga aagttaagaa agtatttttc ttacctgaag
    21721 gcaaagaggc agtagaattg gcttttgaac aaacggatag tcatgttatc tttacagcac
    21781 ctcactttac tcattatgcc tttgtttatg aatctgctga aaaaccacaa cctgctaaac
    21841 cagcaccaca aaacacagtc cttccaaaac ctacttatca accgacttct gatcaacaaa
    21901 aggctcctaa attggaagtt caagaggaaa aggttgcctt tcatcgtcaa gagcatgaaa
    21961 atactgagat gctagttggg gaacaacgag tcatcataca gggacgagat ggactgttaa
    22021 gacatgtctt tgaagttgat gaaaacggtc agcgtcgtct tcgttcaaca gaagtcatcc
    22081 aagaagcgat tccagaaatt gttgaaattg gaacaaaagt aaaaacagta ccagcagtag
    22141 tagctacaca ggaaaaacca gctcaaaata cagcagttaa atcagaagaa gcaagcaaac
    22201 aattgccaaa tacaggaaca gctgatgcta atgaagccct aatagcaggc ttagccagcc
    22261 ttggtcttgc tagtttagcc ttgaccttga gacggaaaag agaagataaa gattaaatat
    22321 cgaaaaatct tgtgaaatct ttccttatat ttccaaagtg tgatataata gtttcgaata
    22381 aaataaataa aggagttttt gtaacatggc aaaacttact gttaaagacg ttgacttgaa
    22441 aggtaaaaaa gtcctcgttc gtgttgactt caacgtacca ttgaaagatg gcgtaatcac
    22501 taacgataac cgtatcacag cagctcttcc aactattaag tacatcatcg aacaaggtgg
    22561 acgtgcaatt cttttctctc accttggacg tgtgaaagaa gaagctgata aagctggtaa
    22621 atcacttgct cctgtagcag ctgacttggc agcaaaactt ggtcaagatg ttgttttccc
    22681 aggtgtcact cgtggtgctg aattggaagc ggcaatcaac gctcttgaag atggacaagt
    22741 tctcttggtt gaaaacactc gttacgaaga tgttgacggc aagaaagaat ctaaaaacga
    22801 tcctgaactt ggtaaatact gggcatcact tggagatggt atcttcgtaa acgatgcatt
    22861 cggtacagct caccgtgcac acgcatctaa cgttggtatc tcagcaaacg ttgaaaaagc
    22921 agttgctggt ttccttcttg aaaacgaaat tgcctacatc caagaagcag ttgaaactcc
    22981 agaacgtcca ttcgtggcta tccttggtgg ttcaaaagtt tcagacaaga tcggtgttat
    23041 cgaaaacttg cttgaaaaag ctgataaagt ccttatcggt ggtgggatga cttacacatt
    23101 ctacaaagca caaggtatcg aaatcggtaa ctcacttgta gaagaagaca aattggatgt
    23161 tgcgaaagct cttcttgaaa aagcaaatgg taaattgatc ttgccagttg actcaaaaga
    23221 agctaacgca tttgctggtt acactgaagt gcgtgacact gaaggtgaag cagtttctga
    23281 aggcttcctt ggtcttgaca tcggtccaaa atctatcgcc aaatttgacg aagctttgac
    23341 tggtgccaaa acagttgtat ggaacggacc tatgggtgta tttgaaaacc cagatttcca
    23401 agctggtaca atcggtgtga tggacgctat cgtgaaacaa ccaggagtta aatcaatcat
    23461 cggtggtggt gactcagctg ccgcagcgat taaccttggc cgtgcagaca agttctcatg
    23521 gattagtacg ggtggtggag catcaatgga acttcttgaa ggtaaggttc ttccaggact
    23581 tgcagccttg acagaaaaat aagattttat aaacaaatca aagaagagag ggatgaaagt
    23641 tcctcttttc ttttgcttaa aataaaaacg cttcctctca actattactc ataaaaatca
    23701 ccgatttatg ataaaatgga aatagaaagt tgagattatg agttatttta aaaaatataa
    23761 attcgataaa tcccagttca aacttggtat gcgaaccttt aaaacaggta ttgctgtttt
    23821 tctagttctc ttgatttttg gcttttttgg ctggaaaggt cttcaaattg gtgctttgac
    23881 agccgttttt agcctgaggg agagttttga tgagagtgtt cattttggga cttcgcgtat
    23941 tctaggaaat agtatcggtg gactctatgc cttggtcttc ttcttattaa ataccttttt
    24001 ccacgaagcc ttttgggtga ccttggtagt tgttccaatc tgcaccatgt taaccattat
    24061 gacaaatgta gccatgaata acaaagcagg ggttattggt ggtgtagcag ctatgttaat
    24121 cattacccta tcaattccaa gtggtgagac aatttcgtac gtgtttgtgc gtgtatcaga
    24181 aacgtttatg ggagtttttg tcgcaattat cgtaaattac gatattgatc gtgttcgtct
    24241 ctttttagag aaaaaagaaa aataatgtta cattttataa cattatcaat tgacgtttgt
    24301 ctttttttag actataatag acagaaagaa ggaaattgta aatgaaggaa aaagaatttc
    24361 gccgaaatat ggctgttttt cctatcggca gtgttatgaa gttgaccgat ctatcggcgc
    24421 gtcagattcg ttattatgaa gatcaagagt tgatcaagcc cgatcgaaac gaaggaaatc
    24481 gtcgcatgta ttccttgaat gacatggatc gtctgcttga aatcaaagat tatatctctg
    24541 aaggttataa tatcgctgcc attaagaaaa aatatgctga acgtgaagcg aaatccaaga
    24601 aagcggttag tcagactgaa gtacgttgtg cacttcacaa tgaactcctc caacaggggc
    24661 gttttgcttc agtacagtca ccttttggtc gcggttaggc aaccgcaagt agtcatagat
    24721 taaggagaaa acttatgcca atcacagctg cagatattcg tcgtgaagtc aaggaaaaaa
    24781 atgttacctt tattcgtctt atgttctcag atattttggg aaccatgaaa aacgtcgaaa
    24841 ttcctgctac agatgaacag ttagataagg tcttgtcgaa caaggttatg tttgatggat
    24901 cttctattga aggttttgta cgtatcaatg agtcggatat gtacttgtac ccggacttgg
    24961 atacatggac agtcttccct tggggagatg aaaatggaag tgttgcaggt ctgatctgtg
    25021 atgtctatac aacagaaggt gaaccatttg cgggtgaccc tcgtggtaat ttgaaacgag
    25081 ctcttcgtca catggaagaa gttggattca aatccttcaa ccttggtcca gagccagaat
    25141 tcttcctatt taagttggat gaaaatgggg acccaacact tgaagtgaat gacaagggtg
    25201 gctactttga tttggcacct actgaccttg cggacaacac acgtcgtgag attgtgaatg
    25261 tcttgaccaa aatgggattt gaagtagaag cgagtcacca cgaggttgcg gttggacagc
    25321 atgagattga ctttaagtac gatgaagttc tccgtgcttg tgataagatt caaatcttta
    25381 agcttgttgt taaaaccatt gctcgcaaac acggacttta cgcaacattt atggcgaagc
    25441 caaaatttgg tattgctgga tcaggtatgc actgtaatat gtccttgttt gatgcagaag
    25501 gaaataacgc cttctttgat ccaaatgatc caaaaggaat gcagttgtca gaaacagctt
    25561 accatttcct aggcggtttg atcaagcatg cttacaacta tactgccatc atgaacccaa
    25621 cagttaactc atacaaacgt ttggttccag gttatgaagc gcctgtttac attgcttggg
    25681 ctggtcgtaa ccgttcgcca cttgtgcgcg tacctgcttc acgtggtatg ggaactcgtc
    25741 ttgagttgcg ttcagtggat ccaatggcga acccttacat tgctatggct gttcttttgg
    25801 aagttggttt gtatggtatt gaaaataaaa tcgaagcacc agctcctatc gaagaaaata
    25861 tctacatcat gacagcagaa gagcgcaagg aagctggtat tacagacctt ccatcaactc
    25921 ttcacaacgc tttgaaagct ttgacagaag atgaagtggt taaagctgct ctcggagatc
    25981 acatctacac tagcttcctt gaagccaaac gaatcgaatg ggcaagttat gcaaccttcg
    26041 tttcacaatg ggaaattgat aattatttag acctttacta atactaatat agaagaaaga
    26101 ttgcctgtgg gtaaacagtc acacagcagt ctaagccatc aaatacctat aacccttaaa
    26161 atagcgattt cggtcgctat ttatttttcg aaaaatcctc ttgactcttt tgcgaattag
    26221 atttatcatc agacgtgtag aagaaaatca aggagtcact atggaaaaat tcaatttcaa
    26281 aaacaatatc gggcaagaaa acaagttact tcaaatcgaa atctacaagt ttactaactt
    26341 ttgcaagctt caaaattaca catctgtaaa tattttttcg aaagatatat ttgaagccat
    26401 agtaaattga gactagaata gtacactaca tctgctaaaa atttctagaa attaatttaa
    26461 ctttcctaat cgatttgttt acatcttatt tcaatctact atattatctc aataaatgat
    26521 gatggcgact gttttgtttg tgcttgtcga tttttgaatc ctgatcgagc ggaagaatat
    26581 ttctgccgag gttgtcaagg ggaaaatcct gaagatattg aattttatga tgaacaatta
    26641 caggcagaaa aggtggaggc tttgaatatt cgattggaag taaaaaattg aattatctac
    26701 aatctcatca acaataaacc tatttcaacc ccattatcta taggcgtatt tttacgcctt
    26761 tttatatact tgaagcacat aagaaacatt atgggaagtt tgctgatgga agtactcaag
    26821 aaattgatgt tccttatgat attcctgata cttgggagtg ggtgaggata aaatcaattt
    26881 attggaattt tgggcaaaat aagccagaga aatcctttag gtatatagat acgtctagta
    26941 ttgatagaaa aaagaacata atcaactaca aaaatctaca atatctttca cctgaacaag
    27001 cgccttcccg tgccagaaaa ttagtttcgc agaatagtgt cttattttca acagttagac
    27061 catatctaaa aaatattgct gtagttagag aacttaaaga gtatttgata gctagtacag
    27121 catttattgt tttggatact ttacttaacg aaacatattt gaaatattat ttattatcag
    27181 ataattttat caatcgtgtt aataataaat ctactggaac aagttatcct gcaatcaatg
    27241 attataattt taatctatta ttaatt
//
LOCUS       DAVBCN010000023        25004 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVBCN010000023 DAVBCN010000000
VERSION     DAVBCN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25004
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.023"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..179
                     /locus_tag="VQ497_000885"
     CDS             <1..179
                     /locus_tag="VQ497_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choline-binding protein D"
                     /protein_id="HET2517220.1"
                     /translation="TGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGLGALAVNT
                     TVDGYKVNANGEWV"
     gene            580..1701
                     /gene="mnmA"
                     /locus_tag="VQ497_000886"
     CDS             580..1701
                     /gene="mnmA"
                     /locus_tag="VQ497_000886"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET2517221.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            1842..2297
                     /locus_tag="VQ497_000887"
     CDS             1842..2297
                     /locus_tag="VQ497_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2517222.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELDVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLQNIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            2307..4220
                     /gene="mnmG"
                     /locus_tag="VQ497_000888"
     CDS             2307..4220
                     /gene="mnmG"
                     /locus_tag="VQ497_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HET2517223.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(4599..6278)
                     /locus_tag="VQ497_000889"
     CDS             complement(4599..6278)
                     /locus_tag="VQ497_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET2517224.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(6280..6513)
                     /locus_tag="VQ497_000890"
     CDS             complement(6280..6513)
                     /locus_tag="VQ497_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HET2517225.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6895..7116
                     /locus_tag="VQ497_000891"
     CDS             6895..7116
                     /locus_tag="VQ497_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517226.1"
                     /translation="MTNQVLEENYLCDSLLAFSPPRDLCRLVCILLWIKCLDALLMLG
                     FFVWGCRDSRFTTRKFHPIYQDEVVFAFF"
     gene            complement(7329..7481)
                     /gene="cibB"
                     /locus_tag="VQ497_000892"
     CDS             complement(7329..7481)
                     /gene="cibB"
                     /locus_tag="VQ497_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HET2517227.1"
                     /translation="MMKDLNNCREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(7483..7668)
                     /gene="cibA"
                     /locus_tag="VQ497_000893"
     CDS             complement(7483..7668)
                     /gene="cibA"
                     /locus_tag="VQ497_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HET2517228.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            7846..8529
                     /gene="tsaB"
                     /locus_tag="VQ497_000894"
     CDS             7846..8529
                     /gene="tsaB"
                     /locus_tag="VQ497_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET2517229.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMESLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLTHTLNIELVGMSSLLALVPY
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMPEAHLSFEEVLEKVKGASQVTFVGEVGPF
                     VEQIQEHLPRTNYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            8526..8963
                     /gene="rimI"
                     /locus_tag="VQ497_000895"
     CDS             8526..8963
                     /gene="rimI"
                     /locus_tag="VQ497_000895"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET2517230.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            8953..9963
                     /gene="tsaD"
                     /locus_tag="VQ497_000896"
     CDS             8953..9963
                     /gene="tsaD"
                     /locus_tag="VQ497_000896"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET2517231.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(10009..11309)
                     /locus_tag="VQ497_000897"
                     /pseudo
     CDS             complement(10009..11309)
                     /locus_tag="VQ497_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(11585..11936)
                     /locus_tag="VQ497_000898"
                     /pseudo
     CDS             complement(11585..11936)
                     /locus_tag="VQ497_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12043..12335)
                     /locus_tag="VQ497_000899"
                     /pseudo
     CDS             complement(12043..12335)
                     /locus_tag="VQ497_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            12578..13784
                     /locus_tag="VQ497_000900"
                     /pseudo
     CDS             12578..13784
                     /locus_tag="VQ497_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            13777..14425
                     /locus_tag="VQ497_000901"
                     /pseudo
     CDS             13777..14425
                     /locus_tag="VQ497_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            14435..16051
                     /locus_tag="VQ497_000902"
     CDS             14435..16051
                     /locus_tag="VQ497_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2517232.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLSGFLRDYSGELRFCGDDIK
                     KTSYLNMVSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNI
                     LDYYVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDRE
                     DLTVIIVTHAPHPELKQYFTKIYQFPKDFI"
     gene            16071..16915
                     /locus_tag="VQ497_000903"
                     /pseudo
     CDS             16071..16915
                     /locus_tag="VQ497_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813546.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16983..18484
                     /locus_tag="VQ497_000904"
                     /pseudo
     CDS             16983..18484
                     /locus_tag="VQ497_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819023.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase"
     gene            18496..19245
                     /locus_tag="VQ497_000905"
     CDS             18496..19245
                     /locus_tag="VQ497_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET2517233.1"
                     /translation="MSDNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSKLKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
     gene            19258..20430
                     /locus_tag="VQ497_000906"
     CDS             19258..20430
                     /locus_tag="VQ497_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET2517234.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKGTLP
                     SKNEELMKFFCENNLNITFFDTFSEIKNNIDYYIIALPTDYDEKIGSFNTYEIEQTVS
                     KILRVKPNGKIILKSTVPIGFSNKLKRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVV
                     GDETVEGRKIAELFLSISTHSTANIKNVMLVSPTEAEAIKLFSNTFLALRVAFFNELD
                     SFAERRSLNAEVVIKGVCLDPRIGNFYNNPSFGFGGYCLPKDTRQLKKEFIEINAPVI
                     EAIDISNTNRKQFIVKQILERKPKIVGIYKLGMKYNSDNYKESAILSIINELLIVGIK
                     ILVYEPNLNVSIDNVIFEKNFELFIKQSDLIVANRWDRGLEAYKDKVYTRGIWIRD"
     gene            20825..21688
                     /locus_tag="VQ497_000907"
     CDS             20825..21688
                     /locus_tag="VQ497_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET2517235.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACAGLTASQLSKFELGQSM
                     LSADKLILAIQGINMNFDEFGHKLNNYQESPHMRIGRRVVDCFSHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKNALHSLDKSYPLTEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRWYMKSGYLNIISELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVLWQFLKKMQIFLQTKGSNQKEIEHFIQSLQVLENPQLTALFE
                     LRLQQYKELID"
     gene            21974..22207
                     /locus_tag="VQ497_000908"
     CDS             21974..22207
                     /locus_tag="VQ497_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517236.1"
                     /translation="MLNLQFAETMELTEAELQDVRGGNLVNSMGGGGRSGISGWGVPG
                     IYPGWGNQGMSPNRGAFDWTIDLADGLFGRRRR"
     gene            22229..22906
                     /locus_tag="VQ497_000909"
     CDS             22229..22906
                     /locus_tag="VQ497_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2517237.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMVALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATSLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            22918..23583
                     /locus_tag="VQ497_000910"
     CDS             22918..23583
                     /locus_tag="VQ497_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2517238.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFFVYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            23655..24881
                     /locus_tag="VQ497_000911"
     CDS             23655..24881
                     /locus_tag="VQ497_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2517239.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRNQPFLNLQTGQSLALLATLEL
                     SALILGSLMSGYLQHTISIKIALYASLLLQLLLLVGFATAHFGLILIFSFLDAFFAGI
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALGTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
BASE COUNT         7715 a         4119 c         5251 g         7919 t
ORIGIN      
        1 caacaggttg ggtgaaagat ggagatacct ggtactatct tgaagcatca ggtgctatga
       61 aagcaagcca atggttcaaa gtatcagata aatggtacta tgtcaatggt ttaggtgccc
      121 ttgcagtcaa cacaactgta gatggctata aagtcaatgc caatggtgaa tgggtttaag
      181 ccgattaaat taaatcatgt taagaacatt tgacatttta attttgaaac aaagataagg
      241 ttcgattgaa tagatttatg ttcgtattct ttaggtacct atcttatgat ttcaggaaat
      301 gtcattaaaa aaacgactcc ttttctctaa cttgaaaaat agattagaga aaatgggttg
      361 ttttatttat tatagttatt tgaatgaaga taagaagaag gtatactcac atcattcaca
      421 taatctgtat gttgactata agtttaaaaa aacaattttt aagctcttcc ttgtcttctc
      481 caaccaagcg tgttataatg aatactgctc aagcgacctt caatcgtgaa gcacacacga
      541 ccttcaatcg tgaataaacg aatagatggg agacttacca tgagtgataa ctctaaaaca
      601 cgtgttgtcg tggggatgag tggtggtgtt gattcgtcgg tgacggctct tttgctcaag
      661 gagcagggct acgatgtgat cggtatcttc atgaagaact gggatgacac agatgaaaac
      721 ggcgtctgta cggcgaccga agattacaag gatgtggttg cggtggcaga ccagattggc
      781 attccctact actctgtcaa ttttgaaaaa gagtactggg accgcgtttt tgagtatttc
      841 ctagcggaat accgtgcagg gcgcacgcca aatccggacg ttatgtgcaa caaggaaatc
      901 aagttcaagg cctttttgga ctatgccatg accttggggg cagactatgt agcgactggg
      961 cattatgctc gagtggcgcg tgatgaggac ggcactgttc acatgcttcg tggcgtggac
     1021 aatggcaagg accagaccta tttcctcagc caactttcgc aagaacaact ccaaaaaacc
     1081 atgttccctc tgggacattt ggaaaagcct gaagtacgca gactagcaga agaagcaggc
     1141 ctttcgactg ctaagaagaa agactcgaca gggatttgct ttatcggaga aaagaacttt
     1201 aaaaactttc tcagcaacta cctgccagct cagcctggtc gcatgatgac tgtggatggt
     1261 cgcgatatgg gcgagcatgc aggtcttatg tactatacaa tcggtcagcg tggcggactc
     1321 ggtatcggtg ggcaacacgg cggtgacaat gccccttggt tcgttgtcgg aaaagatcta
     1381 agcaagaata ttctctatgt aggacaagga ttctaccatg attcgctcat gtcaactagc
     1441 ctagaagcca gtcaagtcca ctttactcgt gaaatgccag aagagtttac gctagaatgt
     1501 acggctaaat tccgttaccg tcagcctgac tctaaggtga ccgtccatgt caaaggagac
     1561 aaggcagaag ttatctttgc ggaaccacaa cgcgcgatta caccaggaca ggcagttgtc
     1621 ttttacgatg gcgaagagtg tctaggtggc ggtttgattg acaatgctta ccgcgatgga
     1681 caagtttgtc agtacattta gattgacaaa ttttctcaat ttgctacaat aataacagca
     1741 atagaaatga tggtcaaagc tcatggatgt tgcaggcttt tttgtcctgc acttctttgt
     1801 agttttgact gtttttgtgt cgtttaaggg aaaggacaag aatgactcaa caagactttc
     1861 ggacaaaagt agacaatacg gtttttggtg ttcgggctac agccttgatt gtccaaaatc
     1921 acaagcttct agttaccaaa gacaagggca agtattacac tattggaggt gcgattcaag
     1981 tcaatgaaag cacggaagac gcggtagtcc gtgaagtgaa ggaagaactg gatgtcaaag
     2041 ctcaagctgg gcagctagct tttgtggttg aaaatcgttt tgaagtggac ggcgtttcct
     2101 atcacaatat cgagtttcat tatctggtgg atttgcttga agatgccccg ttgaccatgc
     2161 aggaagatga gaaaaggcag ccctgtgagt ggattgactt ggataagctc cagaatatcc
     2221 agctagttcc agccttttta aaaacagccc taccagattg ggaaggccaa ctaagacaca
     2281 ttcatcttga ggaataggag agaaacatga cttatcattt tactgaagaa tacgatatta
     2341 ttgtaattgg tgcgggacac gctggggttg aggcttcctt ggccgctagc cgtatgggct
     2401 gtaaggtcct gcttgcgacc atcaatattg aaatgctggc tttcatgcct tgtaatccct
     2461 ctatcggtgg ttctgctaag gggattgtcg tacgtgaagt cgatgccctc ggtggcgaga
     2521 tggccaagac cattgacaag acttacatcc agatgaagat gctcaacaca gggaagggac
     2581 cagccgttcg tgcccttcgt gcgcaggctg ataaggaact ttactctaag gaaatgcgca
     2641 agacagttga aaatcaagaa aatctgaccc ttcgtcaaac catgattgat gagattttgg
     2701 tggaagatgg caaggttgtc ggtgtgcgta cagccaccca tcaagaatat gctgctaagg
     2761 ctgttattgt gacgacaggg actgctctcc gtggggaaat tatcatcgga gacctcaagt
     2821 actcatcagg tcctaaccac agcttggctt ctattaacct agctgacaat ctcaaggaac
     2881 tgggtctcga aatcggtcgt ttcaagacag gaacccctcc acgtgtcaag gcttcttcta
     2941 tcaattacga tgtgacggaa attcagccag gagacgaagt gcctaatcat ttctcataca
     3001 cttcacgtga tgaggattat gtcaaagatc aagtgccatg ctggttgacc tataccaatg
     3061 gtaccagtca tgagattatc caaaacaacc tccaccgtgc gcctatgttt acaggtgtgg
     3121 tcaagggagt ggggcctcgt tactgtccgt cgattgaaga caagattgtg cgctttgcgg
     3181 acaaggaacg tcaccaactc ttccttgagc cagaagggcg caatactgag gaagtctatg
     3241 ttcaaggact ttcaaccagt ctgcctgagg atgtccagcg tgacttggtt cattccatca
     3301 aaggtttgga aaatgcagag atgatgcgga caggttatgc tattgagtat gatatggtct
     3361 tgcctcatca gttgcgtgcg actttggaaa ccaagaaaat ctcaggtctc ttcactgctg
     3421 gtcagacaaa tggaacatca ggttatgaag aagctgctgg ccaagggatt atcgcgggta
     3481 tcaatgcggc tctgaaaatc caaggtaaac ctgagttgat tctaaaacga agtgacggtt
     3541 atatcggggt gatgatcgac gacttggtga ccaagggaac cattgaacct taccgtctct
     3601 tgaccagtcg tgctgaatac cgtctcattc ttcgtcatga caatgctgat atgcgcttga
     3661 ctgagatggg acgcgagatt ggccttgtgg atgatgaacg ctgggctcgt tttgaaatca
     3721 agaaaaatca atttgataat gagatgaaac gcctagacag tatcaaactc aagccagtca
     3781 aggaaaccaa tgctaaggtt gaggaaatgg gcttcaagcc gttgacagat gcggtgacag
     3841 ccaaggaatt ccttcgccgt ccagaagttt cttaccaaga tgtggtggcc ttcatcggac
     3901 cagctgcaga agacttggat gacaagatta tcgaattgat tgaaacagaa atcaagtacg
     3961 aaggctatat ttccaaagcc atggatcagg ttgctaagat gaaacgtatg gaagaaaaac
     4021 gcattccagc caatatcgac tgggatgaca tcgattctat tgcgacggaa gctcgtcaga
     4081 agttcaaact catcaatcca gaaaccatcg gccaagccag ccgtatttcg ggagtaaacc
     4141 cagcagatat ttctattttg atggtgtatc tggaaggtaa aaatcgtagt atttctaaaa
     4201 ctcttcaaaa atcaaaatga tacgtcgtcg gcttcttacg aatgagttca aagcttggct
     4261 ttgattcatc tccaacctcc catagttccc cgaactatgg gagctaactc aagttatgca
     4321 ttttcctagc cttctggtct gattttcatt gagttttaat ctcaaaagaa agcatagaga
     4381 aaaacagctc cgaagacggg gctgttttgt tgacttgtac tcaatgaaaa tcaaagagca
     4441 aactaggaag ctagccgtag gttgctcaaa gcactgcttt gaggttgtag atagaactga
     4501 cgaagtcagc tcaaaacaca gttttgaggt tgtggataga actgacgaag tcagtaacat
     4561 atatacggca aggcgaagct gacgtggttt gaagagtatt attcttcatc tggtgtgagg
     4621 atcatcggga tgatgatcgg ttcacgttcg gtattttcat agaggaaggg gcgaatagcg
     4681 ttgacaatgg caccattgac agattgcacg ctagcatcct tatttttcag tgcgatacga
     4741 atggcattga agaggatacg ctggctttgg cgaatcaagt ctccagactc tctcatgtag
     4801 acaaagcctc ggctgaggat atctgggcca gacagaatca tctgcgattt gaagtcaaca
     4861 gttgcgactg ccagaacgac accgtcttca gatagatcgc gacgatcttt gaggacagct
     4921 gcgccaattt caccgatacg atttccatcg acatagatat cttgggcgtt gaaatgacct
     4981 gcgatacgag ctgagtcagc agtaagggca agcacatcgc cattgctcat gataaagata
     5041 ttgtctttct caacaccagt atccactgct agtccagcgt ggactttttg catgcggtat
     5101 tcaccgtgga caggcatgaa gtattttggc ttaatcaagc ggagcatgag tttttgctct
     5161 tgctgaccac cgtgtccaga tgtatggata ttgttcactt taccgtggat aacttcgaca
     5221 ccagcttcag aaatgatgtt aatcagcttg ttgacgctag tagtgtttcc agggatggga
     5281 ctagaagaga agataacggt atcacctggt tgtaattgta cttgacggtg ggttccgttg
     5341 gcgatacgag agagggctgc cataggctca ccctgactac ctgtacagag gataagaact
     5401 tctcctgcag gataatcttt gatttcattt ggctcgataa aggttccctt aggagctttg
     5461 atgtagccaa gatcgattcc gttgacaatg gccttttcca tagaacgacc aaagaccgca
     5521 atcttgcgtc cagtcttaac agcagcttct gttgcctgct ggagacggaa gatatttgag
     5581 gcaaaggatg caaagatgat acgtccttca ataccttgga taatcttcat aatggactga
     5641 ccaacgactt tttcagagtt ggtaaaggtt ggtacttccg catttgtcga gtcagacagg
     5701 agacagagca cgccttcttc accaagcgca gccatacgat gcaagtccgc aggttctcca
     5761 actggagtaa agtcgaactt aaagtcaccc gtacagacga ttttcccttg aggagtatga
     5821 atgacaatcc ccaaaggctc tggaatagag tgagtcgttc taaagaaagt tgccttgaga
     5881 tttttaaagg tcaactcggt gttgtggttg atttcgtaaa gtttggcgtt gcgcaagagg
     5941 ccgtgttctt cgagtttccc acggatcaaa gccaaggcaa gcggtccagc gtagataggg
     6001 acatttgctt gcttgagtag gaacggaatc ccaccaatgt ggtcctcgtg tccgtgtgtg
     6061 attaaaacag ccttgacgcg gtcgatattg tctacgatgt aagagtagtc aggaatgaca
     6121 tagtcgatac caagcaagtc atcttctggg aatttaatcc cagcatcgac gatgataatc
     6181 tcgtcttggt attcaattcc gtaagtgttt ttcccgattt ctcctagacc accgatggca
     6241 aaaacaccga cttcttcagg tttaagagtg taggccatat tagaactccg taatttcgaa
     6301 ggcgccagtt tctttttcgt aatcgagcaa tttgtcagac aagagttcga tatattcgat
     6361 attgtactct gggcgatttt cttcgacaag ttggcgagca gtgatacggc cctcaagttc
     6421 tgagctggca tcgatgtcta ggtaaagcgc gcgtgttgtt tcacggcgtg ggctacgttc
     6481 ttttgtttct tgataaaaaa ctttgtaaat catatagttc ctttctattt acaggtcagc
     6541 ttattccaaa cttttagcag atatttgctt gatttcattc cattttgtgt ctagattgac
     6601 cttgattcgt tacaattatt tcaattatac cacaaaatga aaaattagta aaagacggaa
     6661 acgagaaagt taggggacga tttcttcggt aagcgtttgc aaaaacaaag aaaatgtgtt
     6721 atgttgataa cggaagtgaa gtttattact tgtcataatt ccatatagat tttaagtcaa
     6781 ggaggttgcc ctatgtataa cttatttgct tttgctatcg gagttcgttt gctagctttt
     6841 atcgtttttg ttttgcgttt atggaggaaa tcttctattt gccaaactgg aacgatgaca
     6901 aaccaagttc ttgaggaaaa ttacctttgt gactctctac tcgctttttc tcctcctcgt
     6961 gacctatgtc gtttggtttg tattttactt tggattaaat gcttagatgc cctgcttatg
     7021 ctggggtttt ttgtttgggg atgtagagat agtagattta cgacacgaaa attccacccc
     7081 atctatcaag atgaggtggt ctttgcattt ttctaaaatg atatgacaat ggaattttca
     7141 aagcttatta tttttatggg agtcattgtt ctgctcaaaa atagagttga agcataaaaa
     7201 taaaacaata caaacagata aatcgaatgg taaagggatg gtagtaatgt tcaaatcatt
     7261 gagaatgctg agcaaactgt aagagatgaa gacaataaaa tttatccttc taaaaatgtg
     7321 tttcttatct agcatgactt accaaacttt ttacgaaggt cttttccaat ggtataacct
     7381 gccataaata aagcgatacc aacacctaca ccaaagccac ccttgatttc ttgcaattcc
     7441 ttattagaaa tttcacgaca gttgttcaaa tctttcatca tattaatctc ctcctaaaaa
     7501 ataaccagct gccaaaccag aacctataag tgctgttcca cctgcaatag ctttccaaga
     7561 tactgctact ccaaagataa ccaagggagc gagaccgcca tcaatatctg ataattcatt
     7621 ttcagataag gataaaaatt gattgtcaag aatgtcaaaa tttgtcattg aactttcctc
     7681 cttttttctt gttcactttt atattcggaa aagaaaagga aaagtgagca tttttttaaa
     7741 ttatttttag ttaagtgagg gcactcttgt ctgggggcaa agaaaattcc catgtgccat
     7801 cttttgtagt ataatagtaa gcagacaaaa agataggaat gtgttatgaa agtattagct
     7861 tttgatacgt ccagcaaggc tctttctctc gctattttag aggataagca ggttcttgcc
     7921 gagacgacga ttaatatcaa gaaaaatcac agtattaccc tcatgccagc cattgatttt
     7981 ttgatggaga gtttggactg gacgcccaag gatttggacc gaatcgtggt ggcggaaggg
     8041 ccaggtagct acacaggctt gcgaatcgcg gtagccactg ccaagacctt gactcatact
     8101 ctgaacattg agttagttgg tatgtctagc ctcttggctc tggtgccata tcaacaagaa
     8161 ggcttgttcg ttcctttgat ggatgcgcgt cgcaacaatg tttatgcagg attttatgaa
     8221 aatgctaaac ctgtcatgcc agaagcgcac ctatcttttg aagaggtgct agaaaaagtc
     8281 aagggtgcta gtcaggtaac ctttgtcgga gaagttggcc cctttgtgga gcagattcaa
     8341 gaacacttgc caaggactaa ttataaagaa accttgccta atgcagccaa tcttgctctt
     8401 ttagcttggg acaaggaagc agactccttg catgattttg tgccaaatta cctcaaacga
     8461 gtcgaggctg aggaaaactg gctcaagaac cataccgagt ctggtgagtc ctacattaaa
     8521 cgcctatgat agaaatcaag cgaattcaac aacagcctga cctagctcaa gccatctacg
     8581 ctgttatggc agctgtttac ctagtcagtc cttggactct ggagcaaatc caagcagatc
     8641 tgtcccaaga ccagacttgg tatgcattgg cttatgatgg ggcagaagtg attggatttc
     8701 tagctgtgca ggagaatctt tttgaagcag aagtcctgca aatcgctgtc aaaggagctt
     8761 atcagggtca gggaattgcg tcagccttgt ttgctcaatt gccgacagat aaggagattt
     8821 tcctcgaagt cagaaagtca aatcaacgag cgcaagcatt ttacaagaaa gaaaaaatgg
     8881 cggtcatcgc tgagcgaaag gcctactacc atgacccagt cgaggacgcc attatcatga
     8941 agagagaaat agatgaagga tagatatatt ttagcatttg agacatcctg tgatgagacc
     9001 agtgtcgccg tcttgaaaaa cgacgatgag ctcttgtcca atgtcattgc tagtcaaatt
     9061 gagagtcaca aacgttttgg tggcgtagtg cccgaagtag ccagtcgtca ccatgtcgag
     9121 gtcattacag cctgtatcga ggaggcattg gcagaagcag ggattaccga agaggacgtg
     9181 acagctgttg cggttaccta cggaccaggc ttggtcggag ccttgctagt tggtttgtca
     9241 gctgccaagg cctttgcttg ggctcacgga cttccactga ttcctgttaa tcacatggct
     9301 gggcacctca tggcagctca gagtgtggag cctttggagt ttcccttgct agccctcttg
     9361 gtcagcggcg gacacacaga gttggtttat gtttcggagg caggagatta taagattgtt
     9421 ggggaaaccc gtgatgatgc ggttggtgag gcttatgata aggtcggccg tgtcatgggc
     9481 ttgacctatc ctgcaggtcg tgagattgac gagctggctc atcaggggca ggatatttat
     9541 gatttccccc gtgccatgat taaggaagat aatctggagt tctccttctc aggtttgaaa
     9601 tctgccttta tcaatcttta tcacaatgcc gagcaaaagg gagaaagcct gtctacagaa
     9661 gatttgtgtg cttccttcca agcagcagtt atggacattc tcatggcaaa aaccaagaag
     9721 gctttggaga aatatcctgt taaaaccctg gttgtggcag gtggtgtggc agccaataaa
     9781 ggtctcagag aacgcctagc agccgaaatc acagatgtca aggttatcat cccccctctg
     9841 cgactctgcg gagacaatgc aggtatgatt gcctatgcca gcgtcagcga gtggaacaaa
     9901 gaaaacttcg caggctggga cctcaatgcc aaaccaagtc ttgcctttga taccatggaa
     9961 taaagagtgg ctctttgtca agtgtagtgg gtagtcgaaa agctgcaatc tggagagtac
    10021 gaaattcgta ctctcttttt tgatgttcaa agtcatcaaa atccgttttt taaaattgtt
    10081 aaagttccgg aaaccaaagg ctttgcgctt gataatctta atcaaattat tggtcgcttc
    10141 aagtttagcg ttggaataag gcagcttaag tgcgttgata atcttatctc tatcccttaa
    10201 aaaagtccaa aagaccgttt gaaaaagcgg atgaaccgtt ggcagttctt gctcaatcaa
    10261 tccaaagaaa tggtcggcat tcttctcttg aaaatgaaag agcaggagtt gatagagttc
    10321 gtagtgaacc tgtaatccct cagagtaaga aagcagctta tctcgtactt cccgatgggt
    10381 taagtgcatt cgaaacgtgg gctgatagaa tcgtttctga cagagcttga agctatactg
    10441 gtgtagtaaa tcagtccagt ggactgattt agcccgagcc tagaaacgaa aaagcgaggg
    10501 ttccaaaagc gcttgagcgc ccgatactcc aaggattttc ggtcaaattg gttcatgatt
    10561 tggattcgta ctcggttcat agctcggctc agatgttgga caatgtggaa gcggtcaaga
    10621 acaatcttcg cctttggaaa gagttgctta gcgagccgat aataagggct gtacatgtcc
    10681 atggtgatga cctcgaccag ctcccgaacc tctctctgat agcgttggaa atggtttcga
    10741 atcaccgcat gagttcgccc gtctaaaatt gcgaggatgg atttggactc aaaatcttgg
    10801 gcaatgaagc tcattttgct ctttttgaag ctatactcat cccaactcat aacttttggc
    10861 aacttggtcc aatcggtttc aaacttaaat tccctcagtt ttcggatgac ggtggaagtt
    10921 gagaccgcca atctgtgggc aatctcagtc atagactgct tttcaaggag gagctgagcg
    10981 attttttgtc tcaccatgtt ggaaatctgg caatttttct tgacaatgga tgtttgagaa
    11041 acgaccacct taaggcaatt cttgcactga aagcggcgct ttttgagatg cagtaccgtg
    11101 ggtaaaccct gacagtcgag aagcggaatt ttagaggctt tctggaagtc gtatttgatc
    11161 atcttccctt gacaatgagg acaaggagga gcgggggaat ccaactttgc ctgaacgact
    11221 agatgggttt ggtatttcag aacagacaag attttgatat ttttgtcttc caatccgagc
    11281 aaatctgtgg tattcttaag ttgttccata tggacccttt ctaatgagtt ggttagacgc
    11341 ttttcattat aggtcatatg gggctttttt tctacaagaa acgaccctat aattcctggg
    11401 gtgggattac ccactacaga aattatagag ccaaagcatt ccaaagtctt gtctgactct
    11461 ggaatgtttt ctgttcacag ttgttttgta actgcctaat aagtaaccgc gctataacga
    11521 ggtatctacc gagattgtca attctccagt atactgttat aaaaaacaaa actggaggat
    11581 ttttttatgt cctcccatcg tcccatcgac tgttccgtag ctgcccaata acggagtata
    11641 gtcggctgaa acaagaatag gacaaaagtg cctcaagaaa agcatcgtaa tttgacaata
    11701 ttattctgag atgtttttga tgtaagccca tattttctca atgggattat actcgggtga
    11761 ataggtagga agtggtaaca gtctatgccc tgctccttac acatgttcat tctgtgaaac
    11821 cttgcattgt ccataatgat aagggatggt gtatctaaag taggtagtaa gaatttttgg
    11881 aagcaagctt cgaaaaagtc actcgtcatc gtatctttat aggtcatcgg agccatagta
    11941 tattgaaaca aaaaagggca ttagtacttc tataacagta tcgcaatctc tggtgtgcgt
    12001 tgtctcgatc ttaattcaaa taattatatc ttcttccaga gagactaact gcctgctcaa
    12061 ggctcgatca tatttgagat aaaaataggt ctcaacccct gtctcataaa tataaatagg
    12121 agtcaggtag cttaagtgat tcaattcttt aaggaaccga tttacttttt ctgggtcttg
    12181 ttcgcagtag gtacggctct ttttagacta tatcccatag cctttagagc gtaatgaata
    12241 gttgttggag gacagttgaa ttcagcagct atttcagtca aataagcatc gggatgagtt
    12301 tcaatataga tctttaattt atctctatca actttttcaa ttaatagtgg aagttttctt
    12361 aaagttagtg tgttgaaacg tttttataca gtgctaaaat ttgaagtgta gtgcagatgt
    12421 atttgtaata gtattatagc aattctttgg agaaggtcga gacaagagaa aataggtatc
    12481 tttgagatat aagtatgatt tactataaaa ttattgagtt attcataaaa atttctctat
    12541 caaatatcat aatgcgtcgt ttaaggagat ggttacaatg caatatagtt gtggaaaaat
    12601 aaatattaat atccctgatg gatatggaga cattaaggat atagtgtttt cggctcatat
    12661 tatagttagg tataataatg gacattgtgg aggtatagat ccgcatataa ttggactttg
    12721 taaaaagcag atcagaagga tgtctccata tcctattttg ataatcgtat ctagagattc
    12781 aaaggttatt gatgattata aaaatttaga tattgcctat gttgattgta ctcaatgttc
    12841 aaataatttt gaaactgcct tacacgttaa aaatatttta aaattattaa aaatccaact
    12901 tatacactgt catggctact caactaatta ttttttatat atgttaaaaa aacttgacaa
    12961 gaatggattt ggaaaggtta agacagtgat aacatgccat ggatgggttg aatataactt
    13021 aaaaaagaaa ttcttgactt attttgattt ttggacatat tctatgggag atgcttttat
    13081 ttgtgtatct gaaactatga aaaaaagatt ggagagtata ataaaaaaat aagaagatag
    13141 ttgcaataaa taacggaatt aatgtatcaa atagcgattt agatgtagta ggtgttcaag
    13201 actttaaaaa agaattttgt attccaaata acaaaaaaat catttgttat gttggaaggt
    13261 tggatccaga aaaaaggccg gatagattcc ttgaatttgc agaaaaatta tttttagtca
    13321 gagaagatgt gatttttatt atggctggaa atggtagtat gtgggctact ttgaaagaaa
    13381 aaatttgcca tttgaaatgt agagataatt tcaggttact tggtgaaatc tatccagcta
    13441 ctatagttta ccaaatatcg gatttattgt atattccttc tgatacagaa ggaataccta
    13501 tgtgtgtttt agagtcaatg tcacaagaaa ctccggtttt agctagtaat gttggcgggt
    13561 taagtgaaat tattgaacat agggttgatg gatttttatt tgagaaggaa gatgttgagg
    13621 gagtgtgtgc ttgtgctaat tttttactca atgattctga gtatttgaaa tatataggtg
    13681 agaatagtaa atcaaaaata agaaaacatt tttctgtgca aaaaatgttt gtagaaacca
    13741 tgagagtata tgatgaatta ttagagaaga gtagtcatgg ataggaatat agatcaagaa
    13801 ttagtaagta ttataatacc aacacataat aggtatgaat ctctgattag agctgtgaaa
    13861 agttgtttac atcaaagtta taaaaatatc gaagttataa ttattgatga taattatagt
    13921 aatgtaaatt taagaaataa aattatccat caatttggct ataccagtca tagaattaag
    13981 ttaattttaa gtaatgaaga tttaggtgca actaatgcca gaaacatagg tatcaaaaat
    14041 tctagaggta agtatatatc atttttagac gatgatgatg aatatatgcc agatcgaatt
    14101 ttaaagttga tggcttgttt taaaaagagt agaatgaaga atttagcttt agtttatagt
    14161 tatggaataa taatttatcc aataggaaat attttaattt attgagtttc aacgagagaa
    14221 tattggttac aaagaaatat tattctttaa acataaagcg gttactatta ataggagaca
    14281 aatgcaaggc tttaaaagta atcaagaagg caagagaaga aaaaattttt aacgaatttc
    14341 tttttcgaaa tatatgttat tatatcattt tggttttttc tattgtatat atgacaacta
    14401 tgttcaatta aaatttagaa agtgagaaac tattgtgtat actattataa aatcaaatat
    14461 aaaaaaattt agtttattaa cgatatttat tgttgctggt caattattgc taatttatgc
    14521 agcaactatt aatgctctgg tgttgaatga attaattgcg atgaatttag agcggttttt
    14581 gaaattgtca atctaccaaa tgattgtctg gtgtgggata atattccttg actgggtagt
    14641 gaaaaattat caggttgaag tgatccaaga gtttaatcta gagattcgaa atagagttgc
    14701 cacagacatc tctaactcta cctatcaaga atttcatagt aaatcatcag gaacatatct
    14761 ttcgtggcta aataatgatg ttcagacttt aaatgatcag gcttttaaac aacttttttt
    14821 agtaataaaa ggaatttctg gtactatatt tgcagttgtg actcttaatc actatcattg
    14881 gtcattgact gtagccacct tgttttcatt aatgattatg ctacttgtac caaaaatctt
    14941 tgcatcgaaa atgcgagaag ttagtctaaa tttaactaac caaaatgaag cttttttaaa
    15001 atctagtgag actatattga atggatttga tgtgttagcg tccttgaatc ttttatatgt
    15061 attgcctaag aaaattaaag aagcaggaat tttattaaag atggttatac aaagaaagac
    15121 aactgtagaa acgttagcag gcgctattag cttctttctc aatatttttt ttcagatatc
    15181 tctcgttttt ttaacaggct atcttgcaat aaaaggaata gtgaaaattg gtactattga
    15241 agcaatagga gcactaacag gtgttatttt tacagcgcta ggtgaattag gaggtcaatt
    15301 atcctctatt attggtacga agcctatttt tttaaaattg tattcaatta atccaattga
    15361 gtcaaataaa atgaatgata tcgaaccaaa tgaggtgaat agagattttc cgttatatga
    15421 agcaaaaaat atttgctata agtatggaga taaagaaata ttaaaaaact taaatttttg
    15481 ttttcaacgt aatgaaaagt atttaatttt aggtgaaagt ggaagcggga aatctacatt
    15541 attaaaatta ttgagtggct ttttgagaga ttatagtgga gaattgcgat tctgcgggga
    15601 tgatataaaa aaaacctcct atttaaatat ggtttcgaat gttctatatg tagatcaaaa
    15661 agcttatttg tttgaaggta cgattagaga taatatttta ttggaagaaa attatactga
    15721 tgaagaaata ctacagtctt tagagcaagt tggtttgagt gtaaaagatt ttcctaataa
    15781 cattttagat tattatgttg gtgatgatgg gagattactg tcaggagggc agaaacaaaa
    15841 aattacttta gctagagggc taattagaaa taagaaaata gtattaattg acgagggaac
    15901 ttctgctatc gataggagaa cttcgttagc gattgaacgt aagatattag atagagagga
    15961 tttgactgtc attattgtta cccatgctcc gcatccggaa cttaaacaat attttactaa
    16021 gatatatcaa tttccaaagg attttattta agaatatagg ggagggaaac atgagattct
    16081 attgtgaagc ttatgaggta attactgaag agaaaaaagt atactttaat gatattgagt
    16141 tagaagttaa gtattcctct gtcgaagaat tgtttataat atgtgagaaa ttattagata
    16201 agaaaaaagt atcatttttt tatgtatatg agaaaccctt aaggtatctg ttatttgact
    16261 atatatttct actagttcta gctaaaaaaa atatcccaat cttagatgga gtttccaata
    16321 agcaagttga tccaacgttg ctgcatcact tttctttgga aattgaaaag aattttatcg
    16381 atttttgcta taaaaatatg gatctcatcc taaaaactca aagtatatct ttgtgtcata
    16441 gagaagagct tattattgta gatgtttcag atgactctaa atttggtgtt ttttacaagc
    16501 gttttagaac actggttgat aaaaataacg gaaaatatgt ttgtgtttat aatttcagtc
    16561 gtgtttcaga tatactgcaa aactggaaaa attattgcaa tcgaaaattt tcggttacgt
    16621 ttactgaaag ccaatttgaa ttatttaagc ttttatataa tcaaaaaaat tttaaaacaa
    16681 tttcgttgct atttggaaaa aaattgttgc tggaggaatt atctattata gcgatcttac
    16741 taatatagaa tacttttgta ttttttggtg ggatagtatg tttgggaaag attctattgg
    16801 aaaatacgta tatgtagaag agattagtag atgtcatttt ttagatagaa attattcttt
    16861 ttgttatggt ttacaagatt ataaaagtaa acttattaaa tattttttag aatagttaat
    16921 ctattcgaga gagataaaga aatatggaaa atactttatt gaatatcaca ggaagcttcg
    16981 atatggaaac taggaatcta attagttatt ctttaactga tatctttgaa acagataaaa
    17041 taagaattga acttcttgga gaaatttatt ataaaaatat aaagttggaa ttgcatgaat
    17101 tcgctggatt gtataagatt tatggaatta gcttaataaa gaatattacc ggaatgtttt
    17161 taattataat ttttgatacg aagacaaaag aattaaaaat ttttcaagat ataacgactt
    17221 catactttaa tttatactat actgtttatg gaggtgtgtt ctatatatag tacatcttta
    17281 aagaaagtga tgaagttatc tcatgttccc gttaccttaa ataacaaaaa aatacaagaa
    17341 tttatgagaa atggatttat tttagattca aatactttag taaccgaaat taataaatta
    17401 gaatatttct cttacatttc tgtaaataat actcttcgta tatgtggtat tgattataat
    17461 gattcaaaca attttactaa agaacaagta ttaaagaatt gggactctat gctacgtgaa
    17521 tctatattaa gagtgtatag tgaagcagga gaggctaata ttacactatc atctggtttt
    17581 gattcaaatt atatattata tacattagcg aattatacga attcttcaat taatgcattt
    17641 tgcataggag gagaaaaagg aattaatgag attcctgaag taactaaaat tgcaaaattt
    17701 tatggtataa atttattagt tgatactgta atgagcccaa gatttagaat attttcccga
    17761 tatagtatgg agattggaag gaagtctctt tgaatgtggt gtcatactgc agtactattt
    17821 aggcaggttg ttgttccaaa aagggaacac aagtattctt tgtggcgagt cagcggatga
    17881 aataatgact tttaagtatc attctgtgaa ctacaatcaa ttttgtaatg ataaacagaa
    17941 gtcagtatat tttagttatt ccgactatcc attttatgtt acaaattcaa ttgttttaaa
    18001 gaaaaatagc ctacttttgc atagttttgg cattcatcct agatatccat ataaaatgtc
    18061 tgagattgtt gaaatgtcta aaaaaatatc agatctaaac gataaaaaag aatttcacaa
    18121 aaagaactgt gagttgaggt ttcaagatag tgttcttgat aacataaata gtgtcccggg
    18181 tacaactcat ttatttagtt gtttaaatat tcaaacatta gtaaaaatta ttttatatat
    18241 ttttcgatac aacagttgta tgaaaatatt taattttaga gataaagagc ttatcttttt
    18301 taataagatt gtaaatgggc tgatacaaaa tattgaggag aatttagagg atgatattga
    18361 aaggatattg aaatatttat atatatgttt atttaacgaa atattcatta ttaagaataa
    18421 ggtgaatttt tttgatgatg tggaatttaa tcagacactt agtgagtttt tagataaatt
    18481 gtagagggaa tggttatgag tgacaatact aagtatatct ttttatctcc acatttagat
    18541 gacgcaattt tttcctgtgg tgattatatt tcaaaattaa cttcagaagg agaaattgta
    18601 cttgttatta ctatattttc tggttaccct ttgagccaac agctacagcc ctctgctaag
    18661 caatttcata agttgtgtaa ccttgggaaa tatccgattg aagaaaggaa gaaagaagac
    18721 aggttagctt gtgaacgtct acaatgtgat tttagacact tatcgtacta tgaatgtctc
    18781 tataggaaag atagaaatgg caattttcta taccgtcata tctatagtaa attaaaaaat
    18841 gaggacacat taaagaatga tatcataaaa gagctgttaa tgcatttaga cgacaaatgt
    18901 gtcgtatatt gtccgctttc acttggtgac catgttgatc atgtttttgt gaatagtatt
    18961 ggaagggcgc ttgaatttat gcgttataaa gtaatatatt atgaggactt cccatatgta
    19021 agtgatagta gtatggttag ttatatggga aaaactaaag aattaaaaat gtatcaagag
    19081 gaacttgatg aaaaacatta tattgataga atatcatcaa tattgtgcta taaatctcag
    19141 attttgataa tctggaaatc agttgaaaaa cttttaaaca atattaaaga gttgtattta
    19201 aggaatgggg cagcttattc tattagattt tggattaaga aataaggaag tagttttatg
    19261 aatatagcag taattggttt ggggcatgtt gggctggcct acgcgttact atttgcatct
    19321 aaatataaag ttgttgcata tgatatagat tctgtaaaaa taaataattt aaaaaagggc
    19381 actcttccat ctaaaaatga agagcttatg aagttttttt gcgagaataa cttaaatatt
    19441 actttttttg atacattttc tgaaattaaa aataatattg attattatat tattgcgctt
    19501 ccgacagatt atgatgagaa aattggtagt tttaatacat atgaaatcga acaaacggta
    19561 tcgaagattc tgagggtaaa acctaatgga aagattattt taaagtcaac agttccgatc
    19621 ggcttttcaa acaaattaaa aaggctgttt gatacaaaaa atatcatttt tgtccctgaa
    19681 tttttgagag aaggttgttc tatatatgat aatttatatc caagtcgcat agttgttgga
    19741 gatgagacag ttgaaggaag aaaaattgca gagttgttcc tttcgattag tactcatagt
    19801 actgccaata ttaaaaatgt tatgttagtt tctcctactg aagcagaagc aattaagctt
    19861 ttttctaaca cattcttagc tctccgtgtt gcttttttta atgaactaga ttcttttgct
    19921 gagaggagaa gtttaaatgc tgaagttgta ataaaaggtg tttgtttaga tccaagaatt
    19981 ggaaattttt ataataatcc ttcttttgga tttggaggat attgtcttcc caaagatact
    20041 agacaattaa aaaaagaatt tatagaaata aatgccccag tgatagaagc gattgatatt
    20101 tcaaacacaa atagaaaaca gttcatagtt aaacaaatat tggaacgaaa gccaaagata
    20161 gtaggaatat ataaattggg gatgaaatat aattcagata attacaaaga gtcagctatt
    20221 ttaagtataa ttaatgaact attgattgtc ggtataaaaa ttttagtata tgaaccaaat
    20281 ttaaacgtta gtatagataa cgtaattttt gaaaagaatt ttgaactatt tataaaacaa
    20341 agtgatctaa ttgttgcaaa tagatgggat aggggccttg aagcttataa agataaagtt
    20401 tacacaaggg ggatctggat aagagattaa ctattgatat taaaaaggca ataatttata
    20461 aaatttgtac aaaaaaatgt taaattttca cattttcata gtatactgtt taatagaaag
    20521 taggttcttt gtaaccgctc cattataaaa tcctttcact cttccggtat actattagga
    20581 aaaacataac tggaggatat tttgagctca cacctcatcg ttccagtggc tgttccccaa
    20641 gctcgtgtac tgcccaaaaa agttagacat tctatttaga acgaggattg agttctgtat
    20701 tgtacaaggc tcggtccttt tagagtcagc ttaaggctgg ctttttcaat caccaaagtg
    20761 tcagaatgtt ttgacaaatg aacacaaata atgatataat atgcaaaagc taggaggtgg
    20821 taggatgatt gaaaaaatgg aactggggga attttacaag gaattgcgct tggcgagaaa
    20881 gctcaagcag tcagatgtgg cttgtgctgg actaacagcc tctcagttgt ccaagtttga
    20941 actaggacag tctatgctgt ctgcagacaa gctgatccta gccatccaag ggatcaatat
    21001 gaattttgat gagtttgggc acaaactcaa caactatcaa gaatctccac atatgcgaat
    21061 tggtagaagg gttgtggatt gcttttccca tcaggatata gctgctttag agcagctatt
    21121 ggaggaagtc gatcaagaac agatggcgca gacctatcgt cgtttgaatg ccattgtgat
    21181 taaaaatgca ctccattcct tagataaaag ctacccgcta acagaggagg atagcgagtt
    21241 tttgactacc tacctctacg ctattgagtc ttggacctgg tttgaactct atcttttttg
    21301 caatactatg cccttcttga gcaatcaaga tctgatcttt ttatcaacct ccttactcga
    21361 aaaatccaag gaatttaaag agttagtaca caatcgatgg tatatgaaaa gtggttatct
    21421 taatatcatc tcagaactta tggagcgcaa gctattttct tatattccaa tctttgaagc
    21481 tgagctggag cgcatgctcc gtccatacga tgtttttgaa aaagtattgt ggcagttttt
    21541 aaagaaaatg cagatttttc ttcaaaccaa aggaagcaat caaaaagaga ttgaacactt
    21601 tatccaatct ctgcaagtat tagaaaatcc acaattgaca gccctttttg aattgcgttt
    21661 gcagcaatac aaagagctta tcgattagtt agaaatggga aaaagataat aatatcagca
    21721 acgatcgtca ataatgttaa aacttgtttc ttcatattac ttccctcctc attagtaata
    21781 actttattat accagggaaa taatcaaatc taccaaaatc gcaaatctga aatttctata
    21841 agaaaaaata tcaaatatgc gattttctaa aataagccaa tttccgtgtt atactgtcct
    21901 tgtaaaacat ttgaagcaaa tcctaggtcg cagaagtggt ttacaaatga agataattga
    21961 aggagtgtaa aagatgttaa atttacaatt tgcagaaaca atggaattga cagaagctga
    22021 gttgcaagat gttagaggag gcaacctcgt aaattctatg ggaggtggtg gaagaagtgg
    22081 tatatctggt tggggcgtcc caggaattta ccctggttgg ggcaatcaag ggatgagtcc
    22141 taatcgtgga gcttttgatt ggactataga tttagcagat ggattgtttg gccgccgtag
    22201 aagataggct cttataggag ataataggat gaagaaaatg aaagaagtga aatttcacct
    22261 agcaactggc ttgttgattt tgacttatta cctcattttt aatgtcacat cagacttaga
    22321 ttttatggtt gctttgtctg ataatatgta ctatgtgttc caagttttat tggtacttat
    22381 tctgggaact atagctacaa ttgcttttgt caaaagtgaa cattggaaag agtgtggtcg
    22441 tttccagttt cgttggtctt acctgggtgt tttccttctt tcttttttct tattgttcgt
    22501 gtgggcaaat ctaacaacct atatctttcc tcgtacccag aatggaagta cagtcgtaga
    22561 agtagccacc agtttaacag gaattagcta ttttgttacg cgaatacttt atactagtat
    22621 tatcgcacct gtatcagaag aggttgtttg tcgtggtctt ttgatgacaa gtttatctaa
    22681 agttaagaga tactatcttg atgttcttgt ttccgctgcc atatttggcg ctatgcatgt
    22741 tttgcagtat ggttggataa cgacagattt cataaagtat tttggaatgg gcttgatatt
    22801 ttgtatgatg tttcgctaca cccgatccat ctattgggct atagctctgc atgcttcatg
    22861 gaatagcttc ttactcattg ttactttatt ggtatttgga tattagaaag gtgatatatg
    22921 aaaaagataa tctcacaccg ctactttatt atagtttttc tactagttat tgctgaccag
    22981 aagttcagtg ttttagtttt acgcagcgac cttgttgctg ggctatctga ctttgcctat
    23041 tatctgtcgg atatgatgtt gaattttctt gtggttttat ttgctcttat tgctatgatt
    23101 tggtcgggga aatggcagaa aatcaatagt agaaagttta aaggatccta tcttttctat
    23161 tccttcctag ctctgcttgc ttttgttgct tggaattttg tcaccttttt tcttttccca
    23221 cctactcgaa atgaaatttc ttatcaacat gctgctccta cttttacagg agctacggca
    23281 tttttgatgt atttttttta tcctgtgatt gcaggtccca tttttgaaga catgatttat
    23341 cgtggattgg tgatgacagc tctggaaaaa ggaaagaaat gggggctaga tgtacttggt
    23401 tctgctattt tatttggggt ctcgcacatt agtaatcacg gttgggtctt gacagacttt
    23461 ttcgtctata tgggtggcgg acttatattt gcagtcttat ttagaatgac aaagtccatt
    23521 tattggccta ttgggctgca tatagtctac aacggcattg gtcagctttt gatgttactg
    23581 taattttggt tgttagtagt atcgcggtct atttctgggg aaagattccc atttgaactt
    23641 aagaaggagt tgtgatgaaa gtatttcttc aaaatagaga ttttaggcaa ttaactatca
    23701 accagtggat ttcaacgctt ggggatacga ttttttatct ggccttcctg aattatgtgg
    23761 cagatgcgtc ttttgcccct ttggcgattt tacttatcac gatttcagaa acccttcccc
    23821 aagttctaca aatctttctg ggagttttgg cggattttca acatcatcgt gtcttgaaat
    23881 acacagtcat tagttttaca aaatttttgc tttactctat agtgtcccta tcgctttcag
    23941 ggcagtcttt ttccttgttg ctagtaacct ttatttgtct gcttaacctc ttgtctgaca
    24001 cattgagtta tttttcaggc gccatgctca ctccgatttt cattagaatt attgggcaag
    24061 accatctggc agaagctatt ggatttaaac agtcaactgt tagtttagtg aaaacaatca
    24121 gtaatatcct aggaggagtc ttactaggca ttctatccat ccagtttatt tccttactga
    24181 acgctcttac ctttttaatc gcatttttag gtatcctctt cataaaaact gacctcttga
    24241 aagtagaaaa aacgattagc tatcaagaag gactctctgt aaaatccttt tgccagcatt
    24301 tgctccaatc atcgaaattg atatggaata tgaataaggt gctcttggtt ttgtttatta
    24361 tctctactag tcaagcagtg ataaatgtta cggttcctat ttccactcta tttttaagga
    24421 accagccctt tttgaattta caaactggtc aatctcttgc cttgttagcc acactagaat
    24481 tatcagccct tattttggga agtctgatga gtggctatct gcaacatacc atttccataa
    24541 aaatagccct gtatgcttca ctcctactcc aattactact cctagtgggc tttgcgacag
    24601 ctcattttgg cttgattctg atctttagct ttttggatgc ctttttcgcg ggtattctct
    24661 ctcctagatt gcaagaactc gtctttaaac aaatacctga ggagtcaatg ggagcagttc
    24721 aatcttctat cggtgccatt acggttgttt tacctagctt atttacaata gctttgggaa
    24781 ccattgctac tagctttgga actctggcag ttagctttgt tttattacta tttcttctag
    24841 ttgcctttgt catgcttctg aatattcgtg aaagtattta gtggagaatt tctataatat
    24901 atgtttgaga ctggattgcc agtcttttta tgatggtcaa aatcaacaga ctttttctct
    24961 tttctgaatg tgttatagta gactgaatct aaaatagtac gaaa
//
LOCUS       DAVBCN010000024        24433 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVBCN010000024 DAVBCN010000000
VERSION     DAVBCN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24433
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.024"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            86..1261
                     /gene="mutY"
                     /locus_tag="VQ497_000912"
     CDS             86..1261
                     /gene="mutY"
                     /locus_tag="VQ497_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET2517240.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     LIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1316..2020
                     /locus_tag="VQ497_000913"
     CDS             1316..2020
                     /locus_tag="VQ497_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2517241.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2013..3362
                     /gene="vicK"
                     /locus_tag="VQ497_000914"
     CDS             2013..3362
                     /gene="vicK"
                     /locus_tag="VQ497_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HET2517242.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3364..4173
                     /locus_tag="VQ497_000915"
     CDS             3364..4173
                     /locus_tag="VQ497_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET2517243.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4264..4506
                     /locus_tag="VQ497_000916"
     CDS             4264..4506
                     /locus_tag="VQ497_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HET2517244.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4508..4762
                     /locus_tag="VQ497_000917"
     CDS             4508..4762
                     /locus_tag="VQ497_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET2517245.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            4809..5582
                     /locus_tag="VQ497_000918"
     CDS             4809..5582
                     /locus_tag="VQ497_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HET2517246.1"
                     /translation="MKKKWFFADYYDTTIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFTTKRKWRFILENIFDLLAILPLNAIFTVFRLGRIFRLAKLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDIVPASIFGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLKDELSSQRILLEKQSKKIEELHKMIQDLIEKT"
     gene            complement(5842..6828)
                     /locus_tag="VQ497_000919"
     CDS             complement(5842..6828)
                     /locus_tag="VQ497_000919"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HET2517247.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGLYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            7022..9490
                     /gene="gyrA"
                     /locus_tag="VQ497_000920"
     CDS             7022..9490
                     /gene="gyrA"
                     /locus_tag="VQ497_000920"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET2517248.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIALRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEE"
     gene            9490..10233
                     /gene="srtA"
                     /locus_tag="VQ497_000921"
     CDS             9490..10233
                     /gene="srtA"
                     /locus_tag="VQ497_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HET2517249.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
     gene            10477..11274
                     /locus_tag="VQ497_000922"
     CDS             10477..11274
                     /locus_tag="VQ497_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HET2517250.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            11422..11544
                     /locus_tag="VQ497_000923"
     CDS             11422..11544
                     /locus_tag="VQ497_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HET2517251.1"
                     /translation="MTCDFKFETLQLHAGQVVAPATKSRAVPIYQTTSFIFDDT"
     gene            11566..12606
                     /locus_tag="VQ497_000924"
     CDS             11566..12606
                     /locus_tag="VQ497_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HET2517252.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFVDVDNLEEVEAAIKDNTKLVLIETLG
                     NSLINIPDLEKLAEIAHKHQIPLVSDNTFAIPYLINVFSHGVDIAIHSATKFIGGHGT
                     TIGGIIVDSGRFDWTASGKFPQFVDEGPSCHNLSYTCDVGAAAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETSSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKDVGSIFTFHVKGGEEEARKVIDNLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPN"
     gene            12777..14051
                     /locus_tag="VQ497_000925"
     CDS             12777..14051
                     /locus_tag="VQ497_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HET2517253.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKDFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            14061..14939
                     /gene="truB"
                     /locus_tag="VQ497_000926"
     CDS             14061..14939
                     /gene="truB"
                     /locus_tag="VQ497_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET2517254.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEIILGYSTTTEDASGEVVAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALTLEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            15207..15482
                     /locus_tag="VQ497_000927"
     CDS             15207..15482
                     /locus_tag="VQ497_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET2517255.1"
                     /translation="MEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQSVMANLSQGL
                     SLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            15590..16228
                     /gene="udk"
                     /locus_tag="VQ497_000928"
     CDS             15590..16228
                     /gene="udk"
                     /locus_tag="VQ497_000928"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HET2517256.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            16356..17696
                     /locus_tag="VQ497_000929"
     CDS             16356..17696
                     /locus_tag="VQ497_000929"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET2517257.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELAIPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            17674..17886
                     /locus_tag="VQ497_000930"
     CDS             17674..17886
                     /locus_tag="VQ497_000930"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET2517258.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            17883..18758
                     /locus_tag="VQ497_000931"
     CDS             17883..18758
                     /locus_tag="VQ497_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET2517259.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLESLQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            18751..19566
                     /locus_tag="VQ497_000932"
     CDS             18751..19566
                     /locus_tag="VQ497_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET2517260.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHSVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            19559..19990
                     /locus_tag="VQ497_000933"
     CDS             19559..19990
                     /locus_tag="VQ497_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2517261.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            19997..21664
                     /gene="recN"
                     /locus_tag="VQ497_000934"
     CDS             19997..21664
                     /gene="recN"
                     /locus_tag="VQ497_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET2517262.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRTIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLDLNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNKMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            21666..22394
                     /locus_tag="VQ497_000935"
     CDS             21666..22394
                     /locus_tag="VQ497_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HET2517263.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYSRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            22473..24296
                     /gene="lepA"
                     /locus_tag="VQ497_000936"
     CDS             22473..24296
                     /gene="lepA"
                     /locus_tag="VQ497_000936"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HET2517264.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMELERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
BASE COUNT         7384 a         4328 c         5664 g         7057 t
ORIGIN      
        1 agatagaaaa gttatatact atctattgaa aatattataa atcagataca aagacaggtg
       61 aaaccttctg tgttatacta gagatatgtt agatttgaaa gaatacggta tcgtcatgtg
      121 gccggaggag aaagtcattt ctttccgtga gaaacttctc gcttggtatg atgaaaacaa
      181 aagagatttg ccttggagga gaagtaaaaa tccttatcac atctgggtat ctgaaatcat
      241 gcttcagcag accagggtgg atacagttat cccttactac gaaagattcc tggactggtt
      301 tccaactgtc gaaagtctgg caactgcgcc tgaggagagt ttactgaaag cttgggaggg
      361 cttgggctat tattctcgag ttcgcaatat gcaggctgca gcccagcaga ttatgactga
      421 ctttggtggc caatttccaa atacctatga aggaatttcc agcttgaaag ggattggacc
      481 ttacacagca ggagccattt ccagtattgc ttttaacttg cctgagccag ctgtagatgg
      541 taatgtcatg cgggtcttgg cgcgtctgtt tgaagtcaac cacgatattg ggattccaag
      601 taatcgaaaa atttttcagg caatgatgga gatcttgatt aatccggatc gtccgggtga
      661 ttttaatcaa gccttgatgg acttagggtc tgatattgag tctcctgtaa atcccagacc
      721 tgaagaaagc ccagtcaagg actttagtgc ggcatatcag aatggaacca tggaccgtta
      781 tccaattaaa tctcctaaga aaaagccggt tcctatttat cttaaagcat tagttgtgaa
      841 aaattctcaa ggacaatttt tacttgaaaa aaatgaaagc gaaaagctct tggcaggttt
      901 ttggcatttc ccccttatag aagttgataa cttttcgcaa gaagagcagt ttgacctctt
      961 tcatcaggtt gcggaagaaa gtgtgaactt tggccccagt ccagaagaga gtttccagca
     1021 ggactatgac ctagatgttg attggcttga tgtttgtttt gatacagtcc agcatgtttt
     1081 tagtcatcga aaatggcatg tacagattgt agcaggtcag gtgagtgact tccatgattt
     1141 ttcagatagg gaagttcgtt ggctttcacc agaagaattc aagaattacc cacttgccaa
     1201 accccaacaa aaaatctggc aggcttatgc acaagccaac ttagacagta gccaagacta
     1261 gctattgtgt cttctgacta ttttttggta taatagtaga gaaaaaggtg aacatatgaa
     1321 aaaaatacta attgtagatg atgagaaacc aatctcggat attatcaagt ttaatatgac
     1381 caaggaaggt tacgaagttg taactgcttt taatggtcgt gaagcgctag agcaatttga
     1441 agcagagcaa ccagatatta ttattctgga tttgatgctt ccagaaattg atggtttaga
     1501 agttgctaag accattcgta agacaagcag tgtgcccatt cttatgcttt cagccaaaga
     1561 tagtgaattt gataaggtta tcggtttgga acttggggca gatgactatg taacgaaacc
     1621 cttctccaat cgtgagttgc aggcgcgtgt taaagctctt ctgcgtcgtt ctcaacctat
     1681 gccagtagat ggtcaggaag cagatagtaa acctcaacct atccaaattg gggatttaga
     1741 aattgttcca gacgcctacg tggctaaaaa atatggcgaa gaactagact taacccatcg
     1801 tgaatttgag cttttgtatc atttagcatc gcatacaggt caagtcatca cgcgcgaaca
     1861 cttgcttgag actgtctggg gttatgacta ttttggtgat gtccgtacag ttgatgtgac
     1921 tgtacgacgt ctgcgtgaga agattgaaga tacgcccagc cgaccagagt atatcttgac
     1981 gcgccgtggt gtagggtatt acatgagaaa taatgcttga tttactgaaa caaaccattt
     2041 ttaccagaga ttttatcttt atcctgattt tgttaggttt catccttgtt gtgaccctct
     2101 tattactgga aaatagacgt gataatattc agttgaagca aatcaatcaa aaggttaaag
     2161 atttgattgc aggagattat tccaaggttc ttgatatgca aggtgggtct gaaatcacca
     2221 atattaccaa taatttgaat gacttgtcgg aggttattcg tctcactcag gaaaatctag
     2281 aacaagagag taagaggcta aatagtattc tgttttatat gacagatggg gttcttgcga
     2341 ccaaccgtcg gggtcagatt atcatgatta acgatacagc caagaagcaa ctggggttgg
     2401 ttaaggaaga tgttctgaat agaagcattt tggaattgct caagatagaa gaaaactatg
     2461 aattgcgtga tttgattacc caaagtccag aattgttgct agattcccaa gatatcaatg
     2521 gcgaatattt gaaccttcga gttcgctttg ccttgatacg tcgagagtct ggctttattt
     2581 caggtttggt ggctgttttg catgatacga cggagcagga gaaggaagaa cgcgaacgaa
     2641 gactctttgt ttccaatgtt agccatgagt tacggactcc tctgactagc gtaaaatcct
     2701 atcttgaagc cttggatgag ggggctttgt gtgaaactgt agcaccagac tttatcaagg
     2761 tttctcttga tgagaccaac cgtatgatgc gcatggtgac ggatctcctc catctttcac
     2821 gtattgataa tgctaccagt cacctagatg tggaactgat taacttcact gcttttatta
     2881 cctttatcct caatcgtttt gacaagatga aaggacagga aaaggagaaa aaatatgagt
     2941 tggtgagaga ttatcccatc aattctatct ggatggaaat tgatacagat aagatgacgc
     3001 aggttgtcga caatatttta aataatgcta ttaagtattc gccagatggg ggtaaaatca
     3061 ctgtcagaat gaagacaact gaagaccaga tgattttatc catttctgac cacggtttgg
     3121 ggattcctaa gcaggattta ccacgtatct ttgaccgttt ctatcgtgtg gatcgtgcta
     3181 gaagtcgtgc acaaggtggt acaggtctag gactgtctat cgctaaagaa attatcaaac
     3241 aacataaggg ctttatttgg gccaagagtg aatacggcaa gggttcaacc tttaccattg
     3301 tgctccctta tgataaggat gcagtgaaag aagaagtatg ggaggatgaa gtagaagact
     3361 agaatgagtg aaataggctt taaatacagt attttagcgt cgggttccag tggaaattct
     3421 ttttatctgg aaacctcaaa aaagaagctt ttagtagatg caggcttgtc tggcaagaaa
     3481 attaccagtc tgctagctga aattaaccgt aagccagaag acctggatgc catcttgatt
     3541 acccatgagc attcagatca tatccatgga gtaggcgttt tggctcgcaa gtatggtatg
     3601 gatctttatg ccaatgaaaa gacctggcaa gctatggaaa atagtaaata tcttggcaag
     3661 gtggattctt cgcaaaagca tatttttgaa atgggtaaaa ccaaaacctt tggagatatc
     3721 gacatcgaga gttttggtgt aagccatgat gcagtcgcac cgcagttcta tcgctttatg
     3781 aaggatgata agagttttgt cctcttgaca gatacaggtt atgtcagtga ccgtatggcg
     3841 ggcattgtcg aaaatgcgga tggttatctt atcgaggcca accatgatgt agagattttg
     3901 cgatcaggtt cttacgcttg gcgcctcaaa caacgaatcc tatctgacct tggtcacctt
     3961 tctaacgagg acggtgctga agctatgatt cggacgctag gaaatcgtac taagaagatt
     4021 taccttgggc atttatctaa ggaaaacaat atcaaggaac tggctcatat gaccatggtc
     4081 aatcagctgg ctcaagctga tctgggagtc ggagtagact ttaaggttta tgatacctca
     4141 ccagataccg caacaccatt gacagagata tagaaagaac gctgagaagg tgttcttttt
     4201 atattgactg gacacctaaa aagtaataca atggtgttac cattaaaaaa gggagcacaa
     4261 aagatgacta ctattacatt aaaagtttct gaagctgata aaacatttat gaaagcaatg
     4321 gctaagtttg aaggagtttc cctttcggaa cttattcgca ccaaaactct tgaagcccta
     4381 gaagatgaat acgatgctcg tgtggcagat ttagcctatc aagagtattt agaagacttg
     4441 gaaaaaggag ttgaacccat tacttgggaa gaaatgatgc atgatttagg cttgaaggat
     4501 gaataatttg tataaattag ttccaacaag acgttttatc aagcaattga aaaaattgga
     4561 ccgttatacg cagaagctaa ttacaaacta tttacaaacc aatgttttgg aagatccaag
     4621 acgacacgga aaggctttgg ttggtaatcg cgttggtcaa tggcgctata gaattggtaa
     4681 ttatcgagtt atcgtacaaa ttgtagatga tgaattagtt gttgctactc tagaagttgg
     4741 tcatcggaga gatatttatt aaattaaagc tgagtaataa cagtatagaa agtaaggaga
     4801 gtaaaaattt gaagaaaaaa tggttttttg cagattatta tgacacaaca attattttac
     4861 ttgctttaat ctctgttatt ctggtattac ttggatttgc tgagatgatt gatttggaca
     4921 atccgcctta tagtattatt gatttagtaa tctggggtgt ttttgtaatt gattatagtt
     4981 ggcgcttttt tacgactaaa agaaaatggc gctttattct tgaaaatatt tttgatttat
     5041 tggctattct ccctttaaat gctattttta cagtgtttcg attagggcgt atctttcgtt
     5101 tagctaaact aacaaaatta ctgaaactga ctcgtttact tagaataatt ggacttacag
     5161 gtaaattaga aagaaaaatt agtagattct tacggacaaa tggcctgatt tatatcttgt
     5221 atgttaatat ctttattgtt ctagtaggga gttcgatttt atccgttgtt gaagaaaaat
     5281 ccttctcaga tagtctttgg tgggctcttg tgacagtaac tactgttgga tatggggata
     5341 ttgttcctgc ttccattttt gggaaatggc tagcagtttt actgatgctt gtcggtattg
     5401 gaacaatagg gatgttaaca agtgctttga cgaacttttt tgtaaaggat aatccagatg
     5461 aacagataaa acttgataaa ctcaaagatg aattatcgag ccaaagaata ttactagaga
     5521 aacaatctaa aaagatagaa gaactacata aaatgataca agatttgatt gaaaaaacat
     5581 aataaaaata aagatgagac aaagtttgag ctgaggagaa atcaagtaag ttttgacaca
     5641 atagaacgca tattatcagg tataaaaact tgatactatg cgttttatag agaaataaaa
     5701 taaaatctga acaaatcaat taggaattta aagcatttta taacaacgtt ttagagtgat
     5761 ggggtgctat ttcaacttca acctactata atacagaaaa aaacaactcc ctgatgattc
     5821 aaggagttgt ctatagttaa attagttttt agaagcttct tggaattctg gatttttcca
     5881 tgcttcgtca ataatagctt gcaattcttt agcagatgct tgcatttttt gagtttctgc
     5941 atcgttcaat ggaatgttta ctggacgaac gataccatgt gcaccaacaa cagctggttg
     6001 accgataaag acattctcaa ctccgtatag accttcttgg aatactgaaa gtggaagtac
     6061 tgcgttttca tcgtcaagga ttgctttagt gatacgagca agggctactg cgataccgta
     6121 gtatgttgca ccttttttgt tgatgattgt gtaggctgca tcacgaacac cttcgaacaa
     6181 ttcaatcaat tcagcttctt gaacattttg agtgtcttta aggaattctt caaggtttac
     6241 accagcgatg ttagcgtgtg accaaacagc gaactcagag tcaccgtgtt cacccatgat
     6301 gtaggcgtgc actgaacgag catccacatc caatttttca gcaagtgctt gacggaaacg
     6361 agctgagtca agtgaagtac ctgaaccgat aacgcgttct ttagggaaac cagagaattt
     6421 ccaagttgag taagtcaaaa cgtcaactgg gttagcagca acaaggaaga tacctttgaa
     6481 accagattca acaacttgag ttacgattga tttgttgata gcaaggtttt tacctacaag
     6541 gtcaagacga gtttcacctg gtttttgagg tgcacctgca gtaatcacaa caaggtcagc
     6601 gtctgcacag tcagagtatt gagctgcata gattttttta ggtgaagtga aggcaagggc
     6661 gtgactaagg tcaagcgcat caccaacagc tttttcatgc aattgtggaa tttcgataat
     6721 tccaagctct tgtgcaattc cttggttaac aagtgcaaaa gcgtaagatg aacctacagc
     6781 accatcaccg acaaggataa cttttttgtg ttgtttagtt gaagtcattg ttttaaacat
     6841 ctccttaatt ttattagggg attttcccta gacaacttca ttctatcact tttaaaaaac
     6901 tttgtcacga atatgcctta tagttctcga tgtaaacgtt ttagtggttt agaggctgaa
     6961 atagatggga atttatggta taatgttgtt acttactaat tgtgaaatga ggcatttatt
     7021 aatgcaggat aaaaatttag tgaatgtcaa tctgacaaag gagatgaagg caagttttat
     7081 cgactacgcc atgagtgtta tcgtagcgcg agctcttcct gatgttcgag atggcttaaa
     7141 acctgttcac cgtcgcattc tctacggaat gaatgaattg ggtgtgaccc cagacaaacc
     7201 ccataaaaaa tctgctcgta ttacagggga tgtcatgggt aaatatcacc cacacgggga
     7261 ttcctctatt tatgaagcca tggtccgtat ggctcaatgg tggagctacc gttacatgct
     7321 tgtagatggt catgggaatt ttggttccat ggatggagat agtgctgccg ctcaacgtta
     7381 taccgaggca cgtatgagca agattgctct ggaaatgctt cgtgatatca acaaaaatac
     7441 agttgatttc gttgataact atgatgccaa tgaacgggaa cccttggtct tgccagcgcg
     7501 ttttccaaac cttttggtta atggagcaac tggtatcgcg gttgggatgg caaccaatat
     7561 tccacctcat aatctgggtg aaaccattga tgcagtgaag ttggtcatgg ataatcctga
     7621 agtgactacc aaggacttga tggaagtctt gcctggacca gattttccaa ctggtgctct
     7681 tgtcatgggg aaatcaggta tccataaggc ttatgaaaca ggtaaaggtt cgattgccct
     7741 acgttctcgt acagagattg aaacgactaa gactggtcgt gagcgcatcg ttgtaacaga
     7801 atttccttac atggtcaata aaaccaaggt gcatgagcat attgttcgct tggttcagga
     7861 aaaacgcatt gagggtatca cagcagtacg tgatgagtca aaccgtgaag gtgttcgatt
     7921 tgttattgaa gtcaagcgcg acgcctcagc caatgttatt ctcaataacc tcttcaaaat
     7981 gacccaaatg caaaccaatt ttggtttcaa tatgctcgct atccaaaatg gtataccgaa
     8041 aattttgtct cttcgtcaga ttttggatgc ttatatcgag caccaaaaag aagtggttgt
     8101 tcgtcgtaca cgttttgata aggaaaaagc ggaagcgcgc gctcatatct tagaaggtct
     8161 cttgattgcg ctagaccata tcgacgaagt gattcgtatc atccgtgcta gtgaaacgga
     8221 tgcggaagct caagctgagt tgatgagcaa gtttaagctt tctgaacgtc aaagtcaagc
     8281 tatccttgat atgcgtcttc gtcgtttgac aggtttggaa cgcgataaga ttcaatctga
     8341 gtatgatgac ctcttggctc tgattgcgga tttagcagat attcttgcta agcctgaacg
     8401 tgtttctcaa attatcaaag acgaattgga tgaagttaaa cgtaaatttt ctgataagcg
     8461 ccgtacagag ttgatgattg gacaggtctt gagtctcgag gatgaggact tgattgaaga
     8521 atcggatgtc ttgattaccc tttctaacag aggctacatt aagcgtttgg atcaggacga
     8581 gttcactgct caaaaacgtg ggggtcgtgg tgtccaagga acgggagtga aagatgatga
     8641 ctttgttcgt gagttagtgt caactagcac ccatgatcat ctgctcttct tcacaaacaa
     8701 gggacgtgtc tatcgtctta aaggttatga aattcctgag tatggtcgga ctgccaaagg
     8761 gctaccagta gtcaatctct tgaaattgga tgaagacgaa agtattcaga cggttatcaa
     8821 tgttgagtct gatcgcagtg atgatgctta tctcttcttt acaacccgtc acggtattgt
     8881 gaagagaacc agtgttaagg agtttgccaa tattcgtcaa aatggtctca aagcgctgaa
     8941 tttaaaggat gaagatgagt taatcaatgt cttgttggca gaaggagata tggatattat
     9001 cattggtacc aagtttggtt atgcagttcg ctttaatcaa tcagccgttc gtggtatgag
     9061 tcgtatcgcc actggtgtga aaggtgttaa ccttcgtgaa ggagacacag ttgttggtgc
     9121 cagcttgatt actgatcaag atgaggttct tattatcaca gaaaaaggat atggtaagcg
     9181 tacagtcgct actgaatacc caacaaaagg tcgtggtggt aagggaatgc agacagctaa
     9241 aattaccgaa aaaaatggct tgctggccgg tcttatgact gttcaagggg atgaggattt
     9301 gatgattatc actgatacag gtgtcatgat tcgaaccaat cttgccaata tttcacaaac
     9361 aggacgtgca actatgggag ttaaagtaat gcgcctggat caagatgctc agatagtgac
     9421 tttcacaacg gttgcggtgg cagaaaaaga agaagttggg acagaaaacg aaacagaagg
     9481 tgaagaataa tgtctcaaaa aaataataaa aagaaaaaca agcgaaaaaa tctgctgaca
     9541 aatatcctag caggatttct gatattactg tcactggctt tgatttttaa tactcaaatt
     9601 cgaaatattt tcatagtctg gaataccaat aagtatcaag ttagccaggt atcaaaagaa
     9661 aaattagaag aaaatcagga tacagaaggc aattttgact ttgattctgt caaagctatc
     9721 tcttcggaag ctgttctaac ttctcaatgg gatgctcaaa aattaccagt tattggggga
     9781 attgcaattc ctgaattgga aatgaatttg ccgattttta aaggacttga taatgttaat
     9841 ctcttctacg gagctggtac aatgaaacgc gagcaagtaa tgggagaagg aaattatagt
     9901 ctagctagtc accatatctt tggtgttgat aatgctaata aaatgttatt ttctccttta
     9961 gataatgcta aaaatggcat gaagatttat ctaaccgata aaaataaagt ttatacttat
    10021 gaaatacgtg aagtcaaacg tgtgacaccg gatcgtgttg atgaagttga tgatagagat
    10081 ggggtcaatg aaatcacatt agtaacctgt gaagaccttg ctgctacaga acgtattatt
    10141 gtcaaaggtg atttgaaaga aacaaaagat tattcacaaa catctgatga aattctaaca
    10201 gctttcaatc aaccatataa acaattttat taatacaaat cagtgaaatc atggatttca
    10261 ctgatttttt aagattttca taaaaatata gtaaaatgaa ataagaatag gacgaatcgt
    10321 tcaggacagt caaatcgatt tctaacaatg ttttagaagt agaggtgtac tattctagtt
    10381 tcaatctact ataaactttt atgaaataca gaaaacgctt cctaaaacct aaagtttgtg
    10441 atataattat caagaaaaaa gatttaggag tttattatgg tttcttcaga atttatctca
    10501 aagattgaat ttgcttgcaa taagaaagaa agtctttata gtcaaagcaa atttaagtat
    10561 gcgattcgtt cgatgttcgc aggtgcattt ttaaccttca gtactgctgc aggtgcagtt
    10621 ggggctgact tgattaataa aattgcacca ggtagtggac gcttcctctt tccattcgtt
    10681 tttgcttggg gcttggccta cattgttttt ttgaatgccg agttggtcac ttcaaacatg
    10741 atgttcttga ctgctggtag tttcttaaaa aaaatctctt ggagaaaaac agctgagatt
    10801 ttactatact gtaccttgtt caaccttatc ggagccttga tagcagggtg gggctttgct
    10861 cattcggcag cctatgcgaa tctgacacac gatagtttca tctcaggtgt tgttgagatg
    10921 aagttaggcc gctcaaatga attggtcttg cttgaggcga ttttggcaaa tattttcgta
    10981 aatattgcga ttctgtcatt tattttggtc aaagatggtg gtgccaaact ttggcttgtg
    11041 ttgtcagcta tttacatgtt tgtattctta acaaacgagc acattgcggc gaactttgct
    11101 tctttcgcga ttgtgaaatt cagtgttgct gcggattcaa ttgccaactt cggtgttgga
    11161 aatatgcttc gccactgggg tgtgactttc atcggaaact ttatcggagg aggcctcttg
    11221 atgggtcttc catatgcctt cctcaataaa aacgaagata cttatgtaga ttaagaaaat
    11281 gagcacgatt gagtcgtgct tttttcattt tcaaaataag gtaatagcta tttcttatat
    11341 caaaatatag aaaactgata tttgtaaact ataactcaag gtgctacaat atccttaata
    11401 aaatgatatg gaggtcacct tatgacttgt gattttaaat ttgaaactct acaactacat
    11461 gctggtcaag ttgtggctcc agctactaag tctcgtgcag tgccgattta tcaaacaaca
    11521 tcttttattt ttgatgacac gtaggaaggt gctgatctgt ttgccttgag gaaaccaggg
    11581 aacatttata ctcgtatcac caatcctaca acagctgccc ttgaaggtgg tgttgaagcg
    11641 ctagcaacag catcaggtat gactgcagtg acttatacga ttttggcgct tgcccatgct
    11701 ggtgaccatg tagtggctgc ttcgactatt tacggtggaa ccttcaatct tttgaaagaa
    11761 ccccttcctc gttatggtat cacaacaacc tttgtcgatg ttgataattt ggaggaagta
    11821 gaagcagcta tcaaagacaa taccaagctt gtcttgattg aaaccttggg taactccttg
    11881 attaatattc cagacctgga aaaactggca gagattgctc ataaacatca aatcccactt
    11941 gtgtcagaca atacttttgc aataccttat ttgattaacg tcttctctca tggcgttgac
    12001 attgccattc actctgcgac taagtttatc ggtgggcatg gtacaactat tggaggaata
    12061 attgtcgata gtggtcgttt tgactggacg gcttcaggga aattccctca atttgttgac
    12121 gagggtccaa gctgccacaa tttgagttat acttgtgatg tgggtgcagc agcctttatt
    12181 atagctgttc gagttcaatt gcttcgtgat acaggtgcag ccttgtcacc attcaatgct
    12241 ttcctcttgc tacaaagact tgaaacctct tcacttcgtg tggaacgcca tgtacaaaat
    12301 gctgagacaa ttgttgattt tcttgtcaac catcctaagg tagaaaaggt aaattatcca
    12361 aaacttgcag atagtcctta tcatgccttg gctgagaaat acttgccaaa agatgtcggt
    12421 tcaatcttta ccttccatgt caaaggtggc gaggaagaag cacgcaaggt cattgataat
    12481 ttagaaatct tttctgacct tgcaaacgcg gcagatgcta aatcgcttgt tgtccatcca
    12541 gcaacaacca ctcacggtca attgtcagaa aaagacctag aagcagcagg tgtcacacca
    12601 aactaaattc gtttgtcaat cggtcttgaa aatgtagaag atttgattga agacttgcgc
    12661 ttggccttgg aaaaaattta aagtaaaaga agataaacag tgggcttcga ctcactgttt
    12721 ttgattttcc ctcaggcatg atataatggt taaagaagtc tagaaagagg aacgatatga
    12781 acgaaatcaa atgtcccaac tgtggggaag tctttacagt aaatgagagt cagtatgccg
    12841 aactcttgtc ccaagtgaga acggcagagt ttgataagga actacacgat aggatgaagc
    12901 aggaactggc cttggctgag caaaaggcca tgaatgagca acagactaaa ctggctcaga
    12961 aggatcaaga aattgcgcaa ttacagagtc agatccaaaa ctttgataca gaaaaagaat
    13021 tggccaagaa agaggttgaa cagacaagcc atgaggctct cttggctaag gacaaggaag
    13081 tacagctctt agaaaatcag ttggctacct tgcgtttgga gcatgaaaat caactgcaaa
    13141 agaccctttc tgacctagaa aaagaacggg atcaggttaa aaaccaacta cttttgcagg
    13201 aaaaggaaaa tgaattatct ttggcttctg ttaagcaaaa ctacgaagcc cagctcaagg
    13261 cagctagtga acaagtcgag ttttataagg attttaaggc tcaacaatct acaaaagcga
    13321 ttggggaaag cctagaacag tatgcagaga gtgagtttaa caaggttcgt agtttcgcct
    13381 ttccaaatgc ttactttgag aaggataaca aggtctcttc gcgtgggtct aaaggggact
    13441 ttatcttccg tgagtgtgat gaaaatggag ttgaaatcat ttctatcatg tttgagatga
    13501 aaaacgaagc agacggaaca gagaagaagc acaagaatgc agatttttac aaggaattgg
    13561 acaaggaccg tcgggagaag aactgtgagt atgccgtttt ggtgaccatg cttgaggccg
    13621 ataatgacta ctttaacaca gggattgttg acgtcagcca cgagtatgaa aaaatgtatg
    13681 ttgttcgtcc tcaattcttt atccagttga ttggtctctt acgtaatgcg gcgctaaatt
    13741 ccctaaaata caagcaggag ttggccttgg ttcgcgagca aaatattgac attacgcatt
    13801 ttgaggaaga cttggatgcc tttaagctag cttttgctaa gaactataat tcagcttcga
    13861 ctaactttgg aaaagctatt gatgaaatcg acaaggccat caaacgcatg gaagaggtta
    13921 agaaattcct gactacatct gaaaaccaac tccgtctagc taacaacaaa ttggaagatg
    13981 tctctgttaa aaaattgacc cggaaaaatc caacaatgaa agcgaagttc gaagcactga
    14041 agggggagta gaaagcaaaa atgaacggta ttatcaactt aaaaaaagaa gcaggaatga
    14101 cctcgcatga tgcggttttt aaactgcgta agattttggg aaccaagaaa attggtcatg
    14161 gtggaacctt ggatccggat gtggtgggtg ttttgccgat tgcggttggc aaggcgacac
    14221 gcatggtcga gtttatgcag gacgagggta agatctatga gggggaaatc attctgggct
    14281 attccacgac gactgaggat gctagtgggg aagtggtcgc agaaacccct gttttgtctc
    14341 ccttggatga aaagcttgtt gatgaagcga ttgctagctt gactgggcct attactcaga
    14401 ttccccctat gtattcggca gttaaggtta atggtcgcaa gctctatgag tatgcgcgtg
    14461 ctggtcagga agtggagcgt ccagaacgtc aggtgaccat ttatcaattt gagcgaacaa
    14521 gtccgatttc ttatgatggc caacttgccc gattcacttt tcgtgtaaaa tgcagtaaag
    14581 ggacttacat ccgtactttg tcagttgatt tgggtgaaaa gcttggttat gcggctcata
    14641 tgtcccattt gactcgtact agtgctgctg gcttacaatt agaagacgct cttaccttgg
    14701 aggaaattgc tgaaaaagta gaggctgggc aattagattt tctccatcct ttagagattg
    14761 ggacaggtga ccttgtcaaa gttttcctaa ctccagaaga ggctacagaa gttcgctttg
    14821 gtcgttttat tgagctagac caaacggaca aagaactggc tgcctttgaa gatgataaat
    14881 tgttagccat tctagaaaaa cggggcaatc tctataagcc aaggaaggtt tttagctaga
    14941 tcgtttagga ataaaaatcg ggtgatagat aacaattgct tgataaaccc ccatactaat
    15001 agtagaatgg ttttgggaat tataatattc caattgttgc gagttgtaga tactcaaata
    15061 atctatatag aaatttagag gtgtgaaatg aagcaactta aaattctttc agataaatat
    15121 ttagagtcca tgacaggttc tggtgggaac ttaggtctag gatttggtgt gataattcca
    15181 tgatgtgaaa tgagtttcga gaaagggtgg agcaacttct tcaacaaaaa gaaataaatg
    15241 aaaatagtga gttgagtcac ctgtttcgtc ttgctataca aaatttagac agaaatgaaa
    15301 aataccaatc ggtcatggcc aatttgagtc aagggttgtc actttacctc atgacgcatc
    15361 attaccaggc acctaagtct gtcattgatt ttggtttatg gattgccaaa gctcctagcc
    15421 aggaaagagg gagactggct ttcttgcaga tgcttgctca aaccctacag ggctttcgtt
    15481 aaaactgtcg aacatcagag ggaagactct ggtgtttttt gctgatgaga ctataaattt
    15541 tgtaaaaaat gtgatagaat agacgacgga taaaataacg gaggatagca tgcaaaatag
    15601 accaatcatt attggagtga caggtggttc tggtggtggt aaaaccagtg tttcaagagc
    15661 cattttatcg cattttcctg atgaaaagat ttccatgatt gagcatgatt catactacaa
    15721 ggatcagtct catttgacct ttgaagagcg tgtcaaaacc aactatgacc atccttttgc
    15781 ctttgataca gacttgatga tcgagcagat taaggaattg ttggcagggc gtccggtgga
    15841 catcccgacc tacgactata cagagcatac acggagtagc aagacctatc gtcaggagcc
    15901 tcaagatgtc tttatcgttg agggcatttt ggtcttggag gacaagcgtc tgcgcgattt
    15961 gatggatatc aagatttttg tggatacgga tgacgatgtg cgtattattc gtcgtatcaa
    16021 gcgtgatatg gaggaacgtg gccgtagcct tgatagcgtt attaaccagt acttaggtgt
    16081 ggtcaaacca atgtaccacc agtttatcga gtcaactaag cgttatgctg atatcgtcat
    16141 tcctgaaggg gttagcaata ccgtggctat cgacctgttg acgaccaaga ttgcaaagat
    16201 tttggaagaa gctcgaaaca gcaaataatc acatgtggag gccttgcctc ctttttctat
    16261 ttttccctca taatggtaca taaatggaga tttttaggca tatttttggt ataataatac
    16321 ccataaaaga gcaagtaaat gaatagtagg tggagatgga aaagtattta tcggtaacaa
    16381 ctttgaccaa atatctgaaa atgaaattcg ataaagaccc atacttggaa cgggtctatt
    16441 taactggtca agtttccaac tttcgtaaac gacctactca ccaatatttc tccctaaagg
    16501 atgaccatgc agttattcaa gcgaccatct ggtctgggat ttatcagaaa ttagggtttg
    16561 acctggaaga aggaatgaag atcaatgtga ttgggcgtgt acaggtctat gaaccaagtg
    16621 gtagctactc catcatcatt gaaaaagctg agcctgatgg ggttggggcg cttgcgattc
    16681 agtttgaaca acttaagaaa aaattgacag aagaaggcct gtttcaagaa cgcttcaagc
    16741 aagctctacc ccaattttct aagagaattg gggtagtaac cagccgtagt ggagccgtta
    16801 ttcgagatat tatcacgacc gtcagcaggc gattcccagg tgttgacatc cttctttatc
    16861 ctaccaaggt tcaaggtgaa ggggctgcgg aggaaattgc tagaaatatt gcgcgtgcta
    16921 atcaacggga cgatttggat ttgctcatta ttggtcgtgg aggtggttct attgaggatc
    16981 tctgggcctt taacgaagaa attgtggtac gagctatttt tgaatctcgt ttgccagtta
    17041 tctctagtgt ggggcatgag acggatgtga ccttggcaga ttttgtggca gatcgacgcg
    17101 ctgcaacgcc aacagcggcg gctgaactgg caatacctgt gaccaagttg gatgtattag
    17161 ctcatttgca aaatcaggaa aaacggatgg caacggcagt ccgaaatgtt ctatctaaga
    17221 aacaagaggc tttgaaaaaa tgcagtcagt ctgttatctt tagacaacct gagcgcttgt
    17281 atgacggtta tttgcaacgc ttggaccaac tgcaactgcg tttgaaacaa agtttgcgaa
    17341 ctcggatttc tgataacaaa caattagttc aagcaagaac tcatcaatta gtacaattat
    17401 cacctgttac caaaatccaa cgctatcaag accgtttagg acagttggac aagctcttag
    17461 gtagccaaat ggcgttagtt tatgacgcca aggttgctga ggccaagcga ctttcggaag
    17521 ctttgctcat gttggatact agccgaatcg tggcgcgtgg ttatgctatt gtcaaaaaag
    17581 aagagtccgt tgtagattcg gttgagagtt tgaagaaaaa agaccaagta acgcttttga
    17641 tgcgagatgg tcaagtagaa ttagaggtta aagatgtcaa aacaaaagaa atttgaggaa
    17701 aatctagcag aactggaaac cattgtccaa agtttggaaa atggtgaaat tgctctggaa
    17761 gatgcgatta ctgcctttca aaagggcatg gtcttgtcaa aagagctcca agctacgctg
    17821 gacaaggctg aaaagacctt ggtcaaggtc atgcaagaag acggaacaga aagtgatttt
    17881 gaatgaaaaa gcaagaaaaa ttagctcttg tcgagtcggc tttggaagat ttttatggag
    17941 accagcagtt tgcctctagt ttacgggagt ctgttctcta ttctattcat gctggtggca
    18001 agcgtattcg gccttttctc ttgttagaag ttctggaatc cttgcaggtt accatcaaac
    18061 ctgctcacgc gcaggtagct actgccttgg agatgattca tacagggagc ttgattcacg
    18121 atgaccttcc tgctatggat gatgacgatt atcgaagagg gcggttaacc aatcacaaga
    18181 aattcggtga agctatggcc attttggctg gagatgcctt attcttagac ccatatgcct
    18241 tgattgcgca ggcagatttg ccaagtcaga tcaaggtgga cttgattgcc aacttatccc
    18301 ttgcttcagg tagtctgggt atggtggcag ggcaagtttt ggatatggag ggcgaacacc
    18361 agcacttgtc tctggaagaa cttcagacta ttcatgccaa taagactggg aagttactag
    18421 cctatccctt ccaagcggca gctattatag ctgaattgtc acctgaaatg caggtgaagc
    18481 tgaaaactgt gggtgaattg attggacttg cttttcaagt cagagatgat gtactggatg
    18541 tgacagctag ttttgaggaa atcggcaaga cacctcaaaa ggatctgcag gcagaaaaat
    18601 caacctatcc tgccttgttg ggcttggaag agtccattgc cttttgtaac caaaccctgg
    18661 atcaagctaa tgaaaaatta gaagaaattg cccagcagct tccctttgaa acagaatcga
    18721 ttgtaagtgt agtagaaagt ttgagaatca atggctaagg aaagagtgga tgtactagct
    18781 tataaacagg ggttgtttga aacgagagag caggccaagc gaggtgtgat ggctggtcta
    18841 gtcgtagcag tccttaatgg agaacggttt gacaagccag gagagaaaat tccagatgac
    18901 accgaattaa aactcaaggg ggagaaactc aagtatgtca gccgtggtgg tttgaaactg
    18961 gaaaaggcct tgcaggtctt tgatttgtcg gtggatggcg cgactacgat tgatatcggg
    19021 gcctctactg gaggttttac cgatgtcatg ctacagaata gtgccaagtt ggtctttgca
    19081 gtcgatgttg gtaccaatca gttggcttgg aaattacgcc aagacccacg agttgtcagc
    19141 atggagcagt tcaattttcg ctatgctgaa aagactgatt tcgagcagga gccgagcttt
    19201 gccagtattg atgtgagttt catttccctt agtctgattt tgccagcctt gcacagtgtc
    19261 ttggctgatc aaggtcaggt ggtagcactt gtcaaacctc agtttgaggc aggacgtgag
    19321 cagattggga aaaatggaat tattcgagat gctaagattc atcagaatgt ccttgaatct
    19381 gtaacagcta tggcagtaga ggcaggtttt tcagtccttg gcttggactt ttctcccatc
    19441 caaggtggac atggaaatat tgaattttta gcgtatttga aaaaagaaaa gtcagcaagc
    19501 aatcagattc ttgctgagat taaagaagca gtagagaggg cgcatagtca atttaaaaat
    19561 gaataaaaaa gaaagacttg aaaaaattag aagattggtg acagattatc aaatcggcac
    19621 gcaagaagaa attgtagaac atttgaaaga agcaggtatc actgccactc aggcgacggt
    19681 atcccgagat atcaaagagt taggtattgt caaaattcct ttgagagaca acacctatgt
    19741 ctacgaattg ccaaaatcaa tcgtaaaaag tctgcaactg gctgaagaca atatcgaatc
    19801 ggctgaattg atggataaga tgatcaatct ccaagttatt ccaggaaata cggcttttgt
    19861 aaaagctcag ttaatcgaga cttttgcaga caagattttt agctgtttga ctgatgatag
    19921 ctcgatttta gtcattgcca gaagtggaag tctagcagag gaaatctttg aacaagtaaa
    19981 aaattggtag gtctatatgt tacttgaaat ttcgataaaa aactttgcca ttattgaggc
    20041 tatttccctc aattttgaaa aggggatgac tgtcctgact ggtgaaacgg gtgcagggaa
    20101 gtcgattatc attgatgcca tgaatatgat gttgggagct cgtgcgacga cagatgttat
    20161 tcgtcatggt gcaccaaagg cagagattga ggggcttttc tcagttgaga atagtcgtct
    20221 tttacaggaa atttttgatg agcaaggttt agaattaggt gatgaaatta tcatccgtcg
    20281 ggaaatcttg caaaatggtc gtagtatcag ccgtgtaaat ggtcagatgg ttaatctgtc
    20341 tgttttgcga actattgggc aacatcttgt agatattcat ggtcagcatg accatgagga
    20401 gttaatgcgt ccccaactgc atatccagat gttggatgaa tttggtgatg ccgctttttg
    20461 ggacttgaaa gaaacctatc aaacgagttt tgatgcctat cggaaaatgc gcaagcaggt
    20521 tctggaagtc aagaaaaacc aacaggaaca caaggcacgt atcgaaatgt tggaatttca
    20581 aatggcagag attgaggcag caaacttgca ggctggagaa gacttggatc tcaatcaaga
    20641 gcgagataaa ctcctcaacc acaaaaatat tgcggataca ctgaccaatg cctacagtat
    20701 gttggacaat gaagattttt caagtctggc caatgttcgt tcagccatga atgacatgga
    20761 aagtgtcgaa gagtatgacc ctgaataccg tgaaatttca agctctctgt ctgagaccta
    20821 ctatgtttta gaagacatta gcaaacgttt ggaagctatc attgaggacc ttgattttga
    20881 tggcaatcgc ctcatgcagg ttgagaatcg tttggacctc cttcatacta ttactcgtaa
    20941 gtatggtggg actgttgatg atgttttgct ttattttgcc aagattacgg aagaatacaa
    21001 tctcttgaca ggcaataatc tttcgtctga ggacatggaa gcagagctta agaagttgga
    21061 agtcaatctt gtcaatttgg caggtcaact tgcttctgct cgtcataatt tggctcagca
    21121 actcgaagct gagattaaac aagaactgca agatctttat atggaaaaag cccagtttca
    21181 ggttcgtttt agtaagggaa aattcagtcg tgagggaaat aaaatggttg agttttatat
    21241 ttcaaccaac cctggagaag actttaaacc cttggttaag gttgcttctg gaggggaatt
    21301 atctcgtctc atgttagcca ttaagtctgc cttttcacgt aaagaaggca agactagcat
    21361 tgtctttgat gaggtggata cgggagtttc aggtcgtgtt gctcaagcta ttgctcagaa
    21421 aattcataaa attggtcagc atggtcaggt tttggctatc tcccatttgc cacaagtaat
    21481 tgcgattgca gattatcaat tctttattga gaagattagt aatgaccatt caacggtttc
    21541 gactgttcgt ctcttgacgg tcgaagagcg agtggaggaa gttgccaaga tgttggcagg
    21601 tgatgatgtg acagaagcag ccctgacgca agcaagagaa ttgttgagaa acagggagaa
    21661 ataagatgac agattattat gtaattggag atgttcacgg aaaagctggg atgctggaag
    21721 accttctcaa aacctgggat ggtcagcccc agttgctctt tctaggtgat ttgattgaca
    21781 gaggtgagga tagtcaccgt gttcttgaaa tggtcaagga cttggtggac aatcaagggg
    21841 ctatctgttt gtccggaaac cacgagtata tgtttttgac ttggctagat gacccagaag
    21901 aaagttatga ccattatagt cgcaatggtg gagatacaac cattaactct atcctaggtc
    21961 gtcccttgga tgcaccagtt gatggagtag aagatgccaa gcgggttgcc gctgaagcgg
    22021 cagacttggt cgaattcatt cgtcaaatgc catttgtagt agagacagac aagtatatct
    22081 ttgttcacgc aggtattgat ttgaccttgg acgactggca tgaaacaaca gattataaaa
    22141 aagtatggct tagaaaacca ttccacggag ctgaaaatca tactggaaaa accattgtct
    22201 tcggtcatac accggtctat ggtttgttac agcaagaacg cggtacatct gagctttgga
    22261 ctacagaaga tggcaagatt ggaatggatg gaggagctgt ttatggtggt gtccttcacg
    22321 ggattgtctt tacagaccaa ggaatgacag aacaccactt tatcgaaaat gacggctttg
    22381 tcgctgaaga ttagtactcc tagcagggta tggtcttgtc aaaatatcaa aaacaattta
    22441 taataaatag ataccctgaa aggaagagaa tcatgaactt agaagaattg aaaaaacgac
    22501 aggagaagat ccgtaacttc tctattatcg cccatattga ccacggaaaa tcaactctag
    22561 cagaccgcat tttggaaaaa acagagacgg tttcaagtcg tgaaatgcag gcccagcttt
    22621 tggatagcat ggagctagag cgggaacgtg gaattactat taagttgaat gccatcgagt
    22681 tgaattacac tgcaaaagat ggggaaactt atattttcca cttgattgac acaccagggc
    22741 acgttgactt tacctatgaa gtttcacgtt cgctagctgc ctgtgagggt gctattttgg
    22801 tggtcgatgc ggctcaagga attgaggctc aaactcttgc caacgtttat ctggctttgg
    22861 ataatgattt ggaaatcatg cctatcatta ataaaattga cctgccggct gcagatccgg
    22921 agcgcgtgcg tacagagatt gaagatgtga ttggtttgga tgccagtgaa gcagttttgg
    22981 cttctgccaa ggctggtatt gggattgaag aaatcctcga gcaaattgta gaaaaagtac
    23041 cagcaccaac gggtgatgtg acggcgccac ttaaggcctt gattttcgac tctgtttacg
    23101 atgcttaccg tggggttatc ctccaagtgc gtgtcatgga cggagtggtc aaacctggtg
    23161 ataagattca gctcatgagc aatagtaaga cctttgatgt ggccgaagtc ggtattttta
    23221 caccaaaagc ggttggtcgt gatttccttg cgactggtga tgttggttac attgcggcgt
    23281 ctatcaagac ggttcaggat actcgtgtgg gtgataccgt taccttggca accaatcctg
    23341 cggcagaacc attacatggt tataagcaga tgaatcctat ggtctttgcg ggtctctacc
    23401 caatcgaatc aaacaagtac aatgacctgc gtgaagcgct tgaaaaattg caactgaatg
    23461 atgctagtct tcagtttgaa ccagaaacat ctcaggcact tggatttggt ttccgttgtg
    23521 gatttcttgg acttctccat atggatgtta tccaggaacg tttagagcgt gagttcaata
    23581 ttgacctcat catgacagct ccgtctgtta tttacaaagt taatttgacc gacggtgagt
    23641 ctatggatgt gtctaaccca tctgagtttc cagacccaac taagattgcg accattgaag
    23701 agccttatgt caaggcgcaa atcatggtac cacaggagtt cgtcggagca gtaatggagc
    23761 tagctcagcg taagcgtggg gactttgtga ctatggacta tattgatgat aaccgtgtca
    23821 atgttatcta tcaaattcct cttgctgaaa ttgtctttga cttctttgat aaacttaagt
    23881 cttcgacacg tggttatgca agctttgact acgaattgtc agaatatcgc ccatctaagc
    23941 tggtgaaaat ggatattctt ctcaatggag ataaggtgga tgccctcagc tttatcgttc
    24001 acaaggactt tgcctacgaa cgtgggaaac tcatcgttga taaactcaag aaaatcatcc
    24061 ctcgccaaca atttgaagtt ccaatccaag cggctattgg acacaagatt gtcgctcgta
    24121 ctgatatcaa ggcccttcgt aagaacgtac ttgctaaatg ttatggtggt gacgtttctc
    24181 gtaagcgtaa actccttgaa aaacaaaaag ctggtaagaa acgcatgaaa tccatcggat
    24241 cagttgaagt tccgcaagaa gccttcctca gcgtcttgag tatggatgaa gaataagtta
    24301 tcagaaatgc ctttccttgt tcacaggaaa tttatataag aatcaatagt aaagcaggct
    24361 tagatggtct gcttttttag tttttatact cttcgaaaat ctcttcaaac cacgtcagcg
    24421 tcgccttacc gta
//
LOCUS       DAVBCN010000025        22982 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVBCN010000025 DAVBCN010000000
VERSION     DAVBCN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22982
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.025"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(76..525)
                     /locus_tag="VQ497_000937"
     CDS             complement(76..525)
                     /locus_tag="VQ497_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET2517265.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(527..1285)
                     /locus_tag="VQ497_000938"
     CDS             complement(527..1285)
                     /locus_tag="VQ497_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET2517266.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(1400..3379)
                     /gene="pknB"
                     /locus_tag="VQ497_000939"
     CDS             complement(1400..3379)
                     /gene="pknB"
                     /locus_tag="VQ497_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HET2517267.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(3376..4116)
                     /locus_tag="VQ497_000940"
     CDS             complement(3376..4116)
                     /locus_tag="VQ497_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET2517268.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVFMNEEDAE"
     gene            complement(4131..5444)
                     /gene="rsmB"
                     /locus_tag="VQ497_000941"
     CDS             complement(4131..5444)
                     /gene="rsmB"
                     /locus_tag="VQ497_000941"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HET2517269.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(5437..6372)
                     /locus_tag="VQ497_000942"
     CDS             complement(5437..6372)
                     /locus_tag="VQ497_000942"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET2517270.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(6385..8781)
                     /locus_tag="VQ497_000943"
     CDS             complement(6385..8781)
                     /locus_tag="VQ497_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET2517271.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLKSQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGTVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(8847..9161)
                     /locus_tag="VQ497_000944"
     CDS             complement(8847..9161)
                     /locus_tag="VQ497_000944"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET2517272.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(9186..9812)
                     /gene="gmk"
                     /locus_tag="VQ497_000945"
     CDS             complement(9186..9812)
                     /gene="gmk"
                     /locus_tag="VQ497_000945"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET2517273.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(9942..11555)
                     /locus_tag="VQ497_000946"
     CDS             complement(9942..11555)
                     /locus_tag="VQ497_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET2517274.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(11746..12000)
                     /locus_tag="VQ497_000947"
                     /pseudo
     CDS             complement(11746..12000)
                     /locus_tag="VQ497_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            complement(12004..12258)
                     /locus_tag="VQ497_000948"
     CDS             complement(12004..12258)
                     /locus_tag="VQ497_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HET2517275.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            complement(12496..13593)
                     /locus_tag="VQ497_000949"
     CDS             complement(12496..13593)
                     /locus_tag="VQ497_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HET2517276.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVLD
                     YQKIADLYTEKGAEMEKFVKNLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQARENVLPEAIHVLGFTEKVRQHLKSLKGQVNLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
     gene            complement(13603..14343)
                     /locus_tag="VQ497_000950"
     CDS             complement(13603..14343)
                     /locus_tag="VQ497_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2517277.1"
                     /translation="MATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLELA
                     CGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGKYD
                     FVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHENA
                     EDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQAG
                     FKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(14663..15016)
                     /gene="rsfS"
                     /locus_tag="VQ497_000951"
     CDS             complement(14663..15016)
                     /gene="rsfS"
                     /locus_tag="VQ497_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET2517278.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(15029..15529)
                     /locus_tag="VQ497_000952"
     CDS             complement(15029..15529)
                     /locus_tag="VQ497_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET2517279.1"
                     /translation="MKTAFIIIDVQNILVETGFQTKSLLGKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(15530..16123)
                     /gene="yqeK"
                     /locus_tag="VQ497_000953"
     CDS             complement(15530..16123)
                     /gene="yqeK"
                     /locus_tag="VQ497_000953"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HET2517280.1"
                     /translation="MSYQDYINCSREALLEKMAEFLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSNQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIANLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            complement(16123..16752)
                     /locus_tag="VQ497_000954"
     CDS             complement(16123..16752)
                     /locus_tag="VQ497_000954"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET2517281.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(16804..17115)
                     /gene="yhbY"
                     /locus_tag="VQ497_000955"
     CDS             complement(16804..17115)
                     /gene="yhbY"
                     /locus_tag="VQ497_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HET2517282.1"
                     /translation="MSLASKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(17329..18435)
                     /gene="yqeH"
                     /locus_tag="VQ497_000956"
     CDS             complement(17329..18435)
                     /gene="yqeH"
                     /locus_tag="VQ497_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HET2517283.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVATRKAII"
     gene            complement(18438..19028)
                     /locus_tag="VQ497_000957"
     CDS             complement(18438..19028)
                     /locus_tag="VQ497_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HET2517284.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(18980..19888)
                     /locus_tag="VQ497_000958"
     CDS             complement(18980..19888)
                     /locus_tag="VQ497_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET2517285.1"
                     /translation="MVLEKQLGHGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(19945..20505)
                     /locus_tag="VQ497_000959"
     CDS             complement(19945..20505)
                     /locus_tag="VQ497_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HET2517286.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            20644..21853
                     /gene="sstT"
                     /locus_tag="VQ497_000960"
                     /pseudo
     CDS             20644..21853
                     /gene="sstT"
                     /locus_tag="VQ497_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            21921..22874
                     /locus_tag="VQ497_000961"
     CDS             21921..22874
                     /locus_tag="VQ497_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET2517287.1"
                     /translation="MSISQRTTKLILATCLACLFAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
BASE COUNT         6160 a         5415 c         4543 g         6864 t
ORIGIN      
        1 tattctcgca catatagaaa gcgttgttaa tttatttatc tcatttttca gaaaattctt
       61 ttatttctgt aaagtctacg atactcgatg tgtttttata taatgataca gtctgagaat
      121 cactgttccg ctagccattc caatagagat taccaaagcc aacatgacaa ccaaggtcgc
      181 acttgccagt gctttattat agtcccctgt cacaaaaaag gcagttgttc ggtaggagag
      241 ataacctgga accagcggtg ccaaaatggc caagataaag accacagcag gtgtcttata
      301 aagaatactt aaaatctggc tgacacaaga accaataatg gctgcaatga aggtagctac
      361 aatgacattg gtcggttcct tgagcaagag atagattagc cagacagtca tgcccaaaat
      421 ccctccaggt aagagcatag accgttgcac attgagtacg attaaaaaag tgataatggc
      481 aagaaaactt gctactgctt gtaataaaaa ggttgttagt gtcatattag ttcatcaata
      541 ccaaggcgac agaagttcct gcccctaaag cgagggtaat gagcagggat tcaaacatct
      601 tactcatacc agagtttatg tggttggtca taatatcacg gaccgcattg gtcaaggcaa
      661 tacctggtac aaacggcatg accgcaccag ctataatcaa atctgccgtt gaaggaaaac
      721 ctgtgtagcg agcccaaaac tgggcaatta tcccaaagac aaaggctcca gcaaaggctg
      781 tcacaaaggg aattcggata aatttttcca catagaggga aaaggcaaaa ccaaataagg
      841 tcgccactcc tgccccaagt gcgtcgtaga tatttccgct aaacataact gaaaagaaag
      901 gagcactaaa ggtcgcagcc agagttacct gcaacttagt atagggaagg ggttgagctt
      961 gcaaagccgt caattgctta aaggctgttt ctaagtcaat ctgcccccca actagctgac
     1021 gagaaatctg gttcacatcg cagacttttt cgatgttata agaagaggag gtcacgcgct
     1081 tcatgcgcga aatattggta ttttcaatag agaaaaagat agcggcaggc atggcaagga
     1141 cattgcaatc cacaatcccc tgcgaatgcg cgattcgaat catggtatct tctacacgat
     1201 ggatttctga gccactttta aggagaatag tccccgctag cataatcaca tcaatgacgg
     1261 catttaattc ttttgattct tccatgcttt cctcctttta tcaactccct ctattctatc
     1321 acaaatccgg actcaaaaaa aatctttgcc atgaaatcat gacaaagatt gattactcat
     1381 tttgattatc catctgcttt taaggagtag ctgaagttgt tttaggtttg tagattgaaa
     1441 tcttgactct agtcttattg aggtctacct tttcacctgc tctaggactt tgttcaacaa
     1501 ccatgccttc tacactacct gcaggcgctg tcgtcacttc tacaacttct atattagctt
     1561 ccttaatccc aacaatttga atcaaattgt tcttagtaaa ctccaagctg gaaccaatgt
     1621 aactcggcat ggcaacactt gtaacttttt tagctactgt caagacaatt tgagtaggtt
     1681 tactcacatc ataagtcgtt ccggcacctg gactttgttt cataatcgtt cctggttcgc
     1741 tttcgctgga ctcttcttcc tctatcttaa tcaaattctc aggaaccttc ttctgcttga
     1801 gttctgagat tacttctgta gagttccgtc caatatagtt ccctaattga atcgtcgtag
     1861 cttttttagc tactgtcaaa acaatttgag ttgccttgct caagtcatag gtcgtacctt
     1921 ctggtagact ttgcttcagg accgttccag cctcactctc attcgactct tcttcctcaa
     1981 ttttaatcaa attatctgga acttttttct cttttaattc cgcgataaca tcagaagatt
     2041 tccggccgac ataattacta atttggaagg attgtttgcc tgatgagaca accagattga
     2101 ttttcgttcc ttcttttcga ccagttccag cgccaggatc tgtacggata atccgccctt
     2161 cttccacctt ttcactagcc tctgtcttct cctcaccaat ctcaaaattg gcttttttga
     2221 gcgttgcctt ggcctctgca actgtctgac ctgccacatc tggaatggca atggttgcag
     2281 gagttctgga tagtatccaa ataagagaag ctgccaccaa tacaaggctg gccaacaaaa
     2341 tcaggtaacg catcttaaat ctatgttttt tcggtgcttg tggttggtaa gtttcctctg
     2401 tcacagcctg gcttgggttt ttgattgatt tgtgttctgt ctgcgcttga accttaggaa
     2461 tagatgtcaa ggtactctga gaaaccttcg gcaaggtctt ggtatctgcc ttgctcgttt
     2521 catcaaagat taacttactt tcatttctac gattgtagga caagctacta gacaagtcca
     2581 catacatctc tgaaaccgag cggtagcgat tggtcaactt tttagcagtt gccttgataa
     2641 taacattttc taaagcctga ggtacagatg gattttctgc aataacggac ggcaggggtt
     2701 tctggaaatg ctggagggca atggtcaccg cgctatcccc gtcataaggg atatggcctg
     2761 tcaacatctc atagaaaata atccccatgg catagatatc actctgcaca gtcgccttcg
     2821 aaccacgcgc ctgctctggt gacaagtaat gaactgagcc caacatcgag ttagtctggg
     2881 tcagacttgt ctctgcaaag gctacagcaa tcccaaagtc tgtgaccttg gcagtcccat
     2941 ctggtgtcaa aaggatattt tgaggtttca agtccctgtg aacaattcct cgagtatggg
     3001 ccaagcgcat agccaagaga atttgtccca tgatacggac tgcttcttca ttagaaagag
     3061 gataatgttc cttgatatag cgtttgaggt ccagtccagc cacatactcc atagctaggt
     3121 actgttgacc gtcttcctcg ccaatatctg ttatccgaac gatatgagga tggtctagat
     3181 ctgccatagc tctcgcttca cgctgaaaac gagctacagc tatcgggtcc gtctggtagt
     3241 tggtcctcag aaccttcact gccacttctt ccccatctaa gattaagtct ttggctaggt
     3301 agacatccgc catgcctcct cgaccaatct gtttgacaat ccgatagcgt ccggcaaaaa
     3361 tcttgccgat ttggatcatt ctgcatcctc ctcgttcata aaaacaaggg caaccgtaat
     3421 gttgtctaaa cctcctgcat tgttagcaaa acgaacaagt gtctccgttt tatctgctaa
     3481 aggaatatca ctggttacaa tatcacgaat ctcactgcct gaaatcatgt tggtcaagcc
     3541 gtcactattg agcaagagat agtcacctga ctcaaggata actgtcccaa aatcaggctg
     3601 aatttcatct ttttgcccaa tagactgggt gataatattt ttttgcggat gagcttctgc
     3661 ctcttctggt gtcaattgac cagccttgag caattcatta accaaggaat gatcgctcgt
     3721 caactgatgg tattcttctc cacgaatcaa gccgatacgc gaatcaccaa tatgagcata
     3781 gatagcctga ttatcaataa tagcaaggac ttccaaagta gttcccatgc ctctgtaagc
     3841 ttcatcctga ccaagctggt gaatcttttg attttcaatt tctaggtaat gggcgaacca
     3901 ttcacgcact tcattgactg tatcgatctg ggtatcaacc caagctacac ccaggtctgt
     3961 gaccgccatt tcactagcga tattccctgc acgatgacct cccatcccat cagctaaaat
     4021 aatcatggta cgtccagctc tattgacata gtggttgaca tagtcttggt tatttgttcg
     4081 tttctgacca acatctgtta ataatgaaat ttccatgtgt cagttccttc ctaatccgat
     4141 atcttgcgaa attgactgat gaagaatcca tcacttccat acaattcagg tgtaatgagg
     4201 atacagccgt ctttcatgat atccttacat tcatgttcta gttttacctg ctcgaactcg
     4261 ggatgactct ctaaaaaggc cttaacgact tgaaaattct cctctgagac gatagtgcag
     4321 gtgctataag ttattatacc acctttgcct agtatttgac aaacactacc taatatttct
     4381 aactgaattt cctgcaagga cgcgaaatct gccgtttctt tattgtattt gatatctggt
     4441 tttcggcgca aaagaccgat tcctgaacaa ggagcatcca ccaaaatctt atcaaaggaa
     4501 tcctggtcaa aaaactcatg cacctttctg gcatccaatt tttgagtttg aacccgatct
     4561 tcaactccca gacgttgggc attttcttga attaaatcca acttgtggtc gtacaagtcc
     4621 agagcagtaa cctgacctgt cgtaagataa gaggctatat gggctgtttt cccacctgga
     4681 gccgcacagg catcaagcac tcgctcatca ccttgtaaat caagcgtcgg agcaaccaac
     4741 tgactggact cgtcttggat ggtaatggct ccatccgcaa acaaattatg ccctgcaaaa
     4801 tgcccctgct ccttaaccag accagtggtt gctaaaaggg aattattcgc ctccaacaag
     4861 gcttggattt cctcttttcg acttaggtct gttacacgaa tactggcttt gtttcgcact
     4921 aacaggcttt caaagatggc ttttgctctc tcctctccgt attcttcctt gagtttggca
     4981 actagccaaa ctgggagaga ataggcaatg gagtcacgct tgttttttcg cttgatgcta
     5041 gcaatatctg gccagccttc acgcaagata cggcgaagga cagcgttgac caatttttca
     5101 ctgccttttt tacggagttt ggccaattcc actgcttcat tgaccacagc atgatctgga
     5161 atcttgtcca aatagcggag ttggtaggca ctcatgagaa gaaggacata gagccagctg
     5221 tctaactggt ctctgtcttc gataaagtgg gataggtacc attccagagt cagtttacgg
     5281 gctaccgttc catagactag ctcggtcact aagcccttgt ctgctgcaga gagctgactc
     5341 cccttgaggt gtttatttaa ggcaatattt gaatatgctt gattcacaaa aacatcctct
     5401 agcactgcta gagctaaact tctagccgtt tctactttag tcaccaaatc gttctcctac
     5461 agtcaatgta cgtccaactc cattgaggaa ggaagcaatg tccatcttag gcttaccagc
     5521 tggctgcact tgtttgaggg atagagcccc ttcagccgtt gcgacaatca attctttctt
     5581 gccgatagag agaatctcac ctggatttcc ctgaccttct actggtaggg cttcataaat
     5641 cttaaagcgg tcgcccttaa ggaaagtatg ggcaacaggc caggggttca ttccacgaat
     5701 ttggttaaag agttgacgat tggttttgtt ccagtccagt ttttcttcct ctggctttat
     5761 atttggagag aaggtaacct gactcgtatc ctgcggttca ggtttgatat caccagcaat
     5821 ataggcaggc agagtgtcca aaagcaaatc acgaccaact agcgccaatt tttcaaacaa
     5881 ggtgccaaca ttgtcctcat ctgtgatcgg aatgctgcga cgagaaatca tatctcctgc
     5941 atccatttcc ttaaccattt ccatgatggt cacaccagct tcctcatccc cttgaatcaa
     6001 ggcataatgg ataggcgcac caccacggtg tctaggaagg agggaggcat gaacgttgac
     6061 agcaaagtcc atgctatcaa ggagtttgct tgggagaaac tgcccaaaag cagcagtcac
     6121 aattccatct gctcctagct tcataagttc ttccatctct ggacttccag ataatttttc
     6181 aggttggtag atagatagtc ctgcttcctt ggcagcctgc ttgactgggg tttcttggat
     6241 aactttttta cgaccaacag cacggtctgg ctgggtcaca acggctagaa tttcgtaacg
     6301 gtcatctgtc aaaagtcctt ttaagactgt tgctgaaaag tcgggggtcc ccataaagat
     6361 tagttttgtc atatcttctc cttcttataa aaattgctgc ggctcatggt caatgctgag
     6421 acggagctca ctattttccc gttcttgagt caaggccaag acctggttga gggtcggccc
     6481 cagctcatct tctaaacggt atttaattaa aatctggtaa tgatagaggt tgtgggtacg
     6541 ggcaatcggt tttggcgttg gccccagaat gggactggtc tctgacaagc ctgaccgcaa
     6601 aatgttcatg acttcatagg cacgtttgaa aacctcttct tctttcttgt gagaaagggt
     6661 aataccaatc gtgaaatagt aaggtggata gccgagttgt cgtctgattc ccatttcata
     6721 ggcataaaag ccttcgtaat cttgatcctt ggcaaatcga atagcatagt gctgcgaatt
     6781 gtaggactgt atcaagactt gacctgcttt ttcagcccga cctgctcgac ctgccacctg
     6841 agtcaagagc tggaaggttc tctcagaaga acggaaatca ggcagattca aggccgtatc
     6901 cgcatttaga actccgacta gggtaacatt gggaaaatcc aaaccctttg caatcatctg
     6961 agtaccaagt aaaatatccg cttcccctcg cccaaactgg tcaagcaagg cttggtgact
     7021 gcctttcttt cgagtcgtat ccacatccat cctcaaaatg cgagcttggg gaaagagttc
     7081 tgctagctca tcataagcct tctgagttcc cgtcccatag taacgaatac tgcggctctt
     7141 acagttagga cagacctgag gaatatcctt cgagaaacca caataatggc agttcatagt
     7201 cttggtatcc atatgcaagg tcagagaaat atcgcagttg ggacaagtat ccaccgtccc
     7261 acactcccga cacatgacaa agctagaata accacggcga ttgagcatga gaaccacctg
     7321 ctctttttta accagacggt cttggatagc ctctagcaaa ggaggcgtaa agtttgacgt
     7381 ctcattttgt ccgatatagt ctcgaaagtc aatcacttga acctcaggga ttgtagccaa
     7441 aggattggca cgttgggtta gacgtaagtg ttgatagacg cctttgccag cacgtgcccg
     7501 gctctctaag ctcggcgttg cagatccaag taccagagtt gcttgattat actgagcccg
     7561 taaaatagct acctctctgg catggtaacg gggattgctg tcctgcttat aagccgcttc
     7621 atgctcttca tcaataatca tgacacccag atttttcaga ggagcaaaga tagcagatct
     7681 ggcaccaaca acaacttggg catcgccacg ctccaccttg cgccattcat catacttttc
     7741 gccattggat aatcctgagt gaagaatggc taccttgtcc ccaaaacgtg ctataaaacg
     7801 ctcggtcatc tgaggagtca aggaaatctc aggtaccagc aaaatagctg tcttaccctt
     7861 atccagggca ccttggataa tctgcaagta aacctcggtc ttcccacttc ctgtaatccc
     7921 ttgaagtaga aagggaggtt gagaactgcc aatagaactc acaaccgcat cacgcgcctg
     7981 tctttgttct ggatttaact ccaaaggtct acttgcttca attccttcaa aataagcagc
     8041 cgagcgttga acttcctttt ggactatggt aacagtacct tgatccacaa agaagttgac
     8101 ttgctctcgc gagtaggact ctaacaagct agccaaggaa gcgctctctg gatgagacag
     8161 cagataatct ctcagttcca actttttctt ggcacgtgta gaaatctcaa caccttctaa
     8221 ttgggcatgg tcaacctcat accaagactg ggtcttgacc ttcttttgat cgactgcctg
     8281 atattccaga ccaagcaggc cttttctagt caaacgcatc atttcagctt gcttggcaag
     8341 gtctagtgaa gaaaaggcta gcgaatcttc tgaaccaaac aggcgtactc gttcttcctg
     8401 actcaagcct tccagaggat agagaatctt gtcatagcta gaattcagaa accctggaag
     8461 catggccttg aggatagaga ttttgtagga gaagacagat ttgcgtaact cctcagccag
     8521 ccagagttgt tctggcgtga gaacaggaga aaaatccagc acctctgcaa tatcttttaa
     8581 atcttgctcc atctcttctc catctgattg ggacttcaaa ccaagaacaa tcccttgaat
     8641 caggcgatta cccttaccaa aaggcacatg aacccgcatc ccaacttcca gcattccctc
     8701 aaattcctcc ggaatcctgt aactataggg ctggtccgtc tgcatcaagg gcacatctac
     8761 gataatctta gctagggcca tcttctcacc tcctccttgt cagtacattc ttgcaataga
     8821 aaaaataaga ttgagtcccc ccaaccttaa attttttcac catcttcttt ttctttagca
     8881 atttgctctt tgattttctt ttcttcttct tctttgcggc gtttttcttc ttcgatacgg
     8941 cgacgcactg cttcacgttt tccttctgga tctgggtgaa ttgtaacgtt tcctgattcg
     9001 atttcttcta aagcgcgaag agttgatttt tcagacttga aaccttgagt tgctggggca
     9061 cctgcttcca attcgtgggc acgttttgct tccaagatta cgagtgaata ttttgaagga
     9121 accttgtcga gcaaggtatc aatagagggt tttaacatca tttgcttgta cctattttct
     9181 aaattttatc gggtagttgg agattttggt aacatctcct gatagtgacc aatgacacga
     9241 tccacacaga agtgttctgc ttcaatcaca catttgacac gttcagcagc taggggcacc
     9301 tgatcgttga caatcgcata atcatactca cgcatgaggg caatttcttc cttggccttt
     9361 tcgattcgtt gggcaatcac ttctgcacta tctgttccac gacctaccaa gcgatcttgc
     9421 aattcatcca aatctggtgg tgtcaggaag ataaagacag catctggaac ctttttcttg
     9481 acctgaagag caccctgaac ttcaatttca aggaaaacat cgattccctt gtccaaggtt
     9541 tcattgacat aggtcagagg agttccatag tagttgccga catattctgc gtattccaac
     9601 atctgtcctt gacgaatcag ctcttcaaat tcttcacgag tacggaagaa atagtcaaca
     9661 ccgtccactt ctccaggacg ttgtgcgcgt gtcgtcatcg atacagaata ttgaaattgg
     9721 ttttcagaac tctcaaaaat ctctcttcta accgttcctt ttccaacccc tgaaggacca
     9781 gaaaaaacga ttagtaagcc tcggtctgcc attgtgtctc cttttagtca atctgtgaaa
     9841 taacatttct ctagaataat ggcaaaaagc cagattatcc tttacagtct ttctatctag
     9901 tgtaacaaaa aagcagtaat ttttcaactg ctctttctta tttatttagc ataatctact
     9961 gcacgaagct cgcgaatcac ggttaccttg atatttcctg gataatcgag attgttttca
    10021 attttcttac gaactttgtg agccaagatt gtgactttgt cgtccttgat ttttcctgga
    10081 ttgaccatga tacgaatttc acgtcctgct tgaagggcaa agctagtttg cactccttca
    10141 aagccgttag caatttcttc caaatcatgg agacgcttga tgtagctttc aagagactca
    10201 ctacgagcac ctggacgggc tgcgctcaag gcatctgctg cagcgacgat aactgctatc
    10261 acgctctcag cttcaacatc tccgtggtga ctagcaatcg tattcaccac aactgggggt
    10321 tccttgtact tacgggccaa ttccataccg atttcaacgt ggctaccttc aacctcatgg
    10381 tcaatggctt tcccgatatc gtgaaggaat ccagcacgac gggcaagagc cgcattttca
    10441 ccaagttcgc tcgccatgat accagccaac ttagcaacct caatcgaatg gcgcaaaaca
    10501 ttttgtccat atgaagtacg gaactgcaaa cgtcccataa tcttcatcaa gtctggatga
    10561 aggtttggcg caccaatttc ataggcagca gcctcaccgt attcacgaat cttattgtca
    10621 atctcttgac ggtttttctc aaccaactct tcgatacgag ctggatgtat acgaccatct
    10681 ttgagcaaca tttccatagt catacgggca atctcacgac gaatcggatc aaatcctgac
    10741 aaggtcacca cttctggtgt atcgtcgata atcacatcga cccctgtcaa actttcaaag
    10801 gtacgaatgt tacgaccttc acgaccaata atgcgtccct tcatagtatc gtctggcaga
    10861 tgaactgttg agtttgttga ctccgctaca tattcaccag cgatacgttg catagcttga
    10921 accaagatgt ccttggccat tttgtcagaa cgttccttga cctcttgctc agcttcgcga
    10981 atgcgactgg caatctccct ggtcaagttt tcctctgtct gagccaagat aatatctcgt
    11041 gcttctgcct gagacagagc accaatacgc tctagttctg cttctttttg tctttcgact
    11101 tcctctaatt gctcttcacg cgcatcaagg tttttcgctc tatcagaaat actttgttct
    11161 ttttgttcaa gtgtttgttc tttactcgtc aaattgtcgt ccttacggtc aaggctagta
    11221 gctctctctg tcaaacgact ttcgatttgt ttgagttctt gacgttctga tttgaattca
    11281 gcgtccactt cttcacggta ttttctggct tcttctttgg cctccaatag tgcttctttt
    11341 ttaagagact tgctttcacg tttggcttca ttaacaagta aatccgcttc acgctcagct
    11401 tgtccacgta aattagttgc ttcttgttca gcatttaaaa gcatcaactc tgcagcttcc
    11461 tgagatgatt tcatcttagc tgagatgctg acatatccaa tgactaaacc aatgatgacg
    11521 gcaaaaacag caatcgcaag cgacatgatt tccatgtttt tacctcattt tattgttatt
    11581 ccgaatgaca tacattcttt tacattctac cataaaaaag tgattttcac aaacctaaaa
    11641 tagaatatgt tttggggaat ttggaacaca tttaccaaaa taaacttgtt gtttagaaat
    11701 agtagtttag tagagacttg agaaaaagcc tactttgcag tagacttagt aatgatcttt
    11761 aaaggacaag aaagccacgc tatctccatc catcatataa atcaagcgat tttctgcatc
    11821 aatacgccgt gaccaggctc cttggtaatc atatttgagt ggctctggtt ttcctattcc
    11881 tgtaaatgga tcgcgttgaa tatccttgat taatttattg attcttttta gtgttttttt
    11941 atcttggttt tgtcagtagc aataatctgc ccaggcatct tctgtaaact tgagtagcat
    12001 tcatcactcc tcaatcacat ggacctgagt gcttccagca cggacttgag ccattcctcg
    12061 caaaaccttg tctgataact ccttattttg ggcgattctc agggtttcct gaatactgtc
    12121 ccattcgctc tttgaaagaa ctacaatgtc ctcatctgga tttttattga ccaccgtcaa
    12181 aggctcaaat tcatcattta ccttcttcat gtagtccttt aaatgatttc ggaatgttga
    12241 gtaaaggact gcttccataa ccatacctcg ttttagctct tttccactat tatacacgaa
    12301 aagaaagaaa ttgtcaggaa cttgtacaag attttctttt ctatctattt atactcaatg
    12361 aaaatcaaag agcaaactag gaaactagcc gcaggctgta cttgagtacg gcaaggcgac
    12421 gttgacgcga tttgaatttg attttcgaag agtattattc gtaaaaaatc tcaaaaagcc
    12481 tacctttcgg tagacttagt ttgtttctat tctaatcggc actcttccaa aattttgctc
    12541 tgctatactt ggcttcccta gttggtaaat ctggtctgct ttttgggtca tagcatccca
    12601 aggttctttg ccaattcggc tgactagatt gacctgtcct ttcagagact tgagatgttg
    12661 cctgactttt tcagtaaatc caaggacatg aatggcttct ggcaagacat tttctctagc
    12721 ctgtaccaaa atataggtca ataggcgtct gacacgcgcc ttggtgtaac gtttggtagt
    12781 cactaactcg accaattctt ccacagactg ggctgtttta atagcttctt taatgcgcac
    12841 agccatttct tgattgacct gatagatggt ggttaggtcg ggatttgaca agatttgata
    12901 gcggagcaag ggaaaatagt cttcccagat caccttactg gcctgctcaa atagggcaac
    12961 agaaggcata aaacgttcta agaaatcttg gtccttctga tgttgacgga gggctgtcgc
    13021 cgaggcaaag tccacatctt tattcacaga atggtaacct gccccctgac gctgaatcgg
    13081 atgcagcttg atatttcgtc ctgcaaccgc cttggcatag gccagagcaa gaacatgatt
    13141 gggggtatta cctgaaaaat caagaccagc aaattccttc cacatagctt gggttttctg
    13201 gggataggaa agggaatcag gcagattttt cacaaatttc tccatctcag cacctttctc
    13261 tgtgtataag tcagcgattt tctggtaatc cagaacttcc tctgtcccaa aaactagact
    13321 atcaatgccc aagcgatcca agatatccac agctccttga ccaaagaaat cagctgcctg
    13381 aacactgact aaaaagggca attccactac caagtctgcg ccattttcca gcgccatctg
    13441 ggcccttgtc cacttatcca caatagcagg ctctccacgc tgcatgaaat tccctgacat
    13501 ggcaacaatt ttcagtccct ctgcctgatc cagcaggtat ttatggccat tatgaaaagg
    13561 attgaactcc gcgataatac ctgttatggt catgctaatc tcctacttct gcgccacaaa
    13621 aaaccaacgg gtgctagttt ctgttggctc cttatcctca aagtctgcat agagtttgaa
    13681 ggacttaaat ccagcctgtt ccagcaagat atcataggtc aagatctcat aggtcctctc
    13741 ctcatgtact tcatcgtggc gactaaagga accgtcagcc tccttgataa agaaagtcag
    13801 ctcatgcacg atggagtgag gaacttcgtc ctcatatgta tcccaaagca tggcaaaatc
    13861 ttccgcattt tcatggtagg aatagccagg gaatacttca tctgtctggt aggtcgagtg
    13921 cacgtcaaag atgaaaactc cctcttcatt cagcgcattg tacacgtcct taaagacgtc
    13981 ccctacttcc acctcatcct gcatatagca gatagagtcc gaataacacg tgacaaaatc
    14041 gtatttccct gccttggaca aattcagcat attaccttct ataaaagcaa tcttttgctt
    14101 ggctgaagtt gctctcttct ccgcaatctt caacatatcc gcactcaagt caagtccagt
    14161 cacatcaaaa ccagcctgag aaaagcgcac tgattgaatt cctgtcccac aagccaattc
    14221 caagagtttc tttctctcct tggtcttagg caaatgacgc agagaaaaat tcgtccattt
    14281 gtcgtataaa ctatcgtcca tgaccgcatc atagaccgcc gcaaaggttt cataagtcgc
    14341 cataaaacat gataccaagt ccgcccgtaa ttcgatagaa aattaggagc cttacgatgg
    14401 agccgagctc caaggaaggc tatctatttt ccgagaatta caggacgggt tcaaatcctt
    14461 tctaagatac aaagagcctt aaaagcaaag agtacatagg aaactttctt ccctatcgtc
    14521 agatacaaag aaagttactt tttatctcag cttttagctc gtgttcagtt tcgaaatgaa
    14581 acaaacacga ggccctttac ctttctatca atttttctaa aataagcaaa gaaacccagt
    14641 tgactacaac tgagttcatc ttctaagcaa gagcttctga aatatctact gaattcgcct
    14701 catgccatag cttctctaga ttataatggg cacgcatttc ttctgaaaag atatgcacga
    14761 caacagcacc aaggtccagt aagacccagc ctccagctgc atcgccttcg acatggctac
    14821 ctttaaagcc tgcttgagct actttttcac ggatattagc tgcgatagcg tccaactgac
    14881 ggctattcat tgagctagtg atgacaaagt agtccgtcac actagtcaaa tcttgtacat
    14941 caagtgcgag gatatcctcc gcacgtttct catcagccgc tttcacgact agttctagta
    15001 attctttttc gttcatttaa tcctcttttt agaaaatatg tttgtaatct agaacatctg
    15061 caaagcgctc ctcccaaatg tcctcataaa attcattaat cgtttctgct ggaatgtcat
    15121 ccccatcaaa ggttgtgaca gcaccttctg gaacaataag ctgatagcca tattcaaagg
    15181 caaccttgac agaggtatcc acacaatatt ctgtctgcat accacataaa actaattttt
    15241 caatcccctg cttatccaag tattctttta agccagtttc tttgaaaata ctgttatact
    15301 tcttctgaaa gaccttttca gcaggttttc gatttaaaag cgcagataac tgccaatctt
    15361 ctgatgtttg agcttcagag ttctcaatat gttgaacata gataatttca atattcttgc
    15421 ttctagcctg gttttgtaaa taagaaattt ttcccaatag actttttgtc tggaacccag
    15481 tttctactaa aatattctga acatcaatga ttataaaagc cgtcttcatt tagttctctt
    15541 tcaaatagtg cacaaaggcg ttataggttt caagggtttg gggatagatg gggaatccct
    15601 gatgagctag atactccacg gtacgagctg tttcgtaggc caccgcctta ttgagcgata
    15661 gatttgcaat ctcacgcgcc acatctactc caggaaaggc tcgattgtgc tcgatatagt
    15721 ctgcgacgta gatgacctta tctaggtctg tcatttgacc agcaccgact gtatggactt
    15781 caatggctcg caggatttct gaatcatgca aatccaaatc ttcctgaatt ttgtaaattc
    15841 cgaccatacc atgccagaca ttattgcccc agtttttgag gtcagggtct agctggtaac
    15901 ggtcaatcaa ggctagaaat tcctgatttg acaacttttt agcatagtca tgaagaagac
    15961 ctgctagact tgctttctca acatctacac caaagcgctg agctaattcc atagcggcac
    16021 gctccacacc tagacaatgg gttagacgtt tctcaggtag aaactctgcc attttttcca
    16081 acaaggcctc acgggagcag ttgatatagt cttgatagga catcagtaga gcccctcctt
    16141 ctcgatgtag tctagcactg gctgaggtag gagaaagttg ggtttccgac cttgggcaag
    16201 gaagtcccgc accatgctgg acgagatatc catgagcggt acgtccaccc agataactgg
    16261 ataggaagtc cctaccttgt agcgtggacg ctgaaccccc acaaactgaa ccatgtcaac
    16321 cagttcatca attcggtacc acttaggcag atagtcaacc atgtcggcac cgatgataaa
    16381 gtaataatcc gtatctggat tcttctctgt caaaatcttc atggtgtcgt aggtgtagga
    16441 aatacccttg cgctccaact caatggtttc aatgactagg ccgtcaattc cctcaattgc
    16501 caactcaagc atcttgagac gatggtgttc agggatggtt tcctttttat caacgtgagg
    16561 aggttggtat tcaggcatga gaagaacttg atccagtccc aactgttgcc gtacttgatc
    16621 cgcaacaatg agatgggcat tgtgaacagg gttaaaattc ccccctaaaa tcccaacttg
    16681 tttgcgtttt ttctccttga tttctggctc caactctacc ttggtaaagg gagtcaatag
    16741 ttcgattgtc ataggctagt cccctttatt tctctataaa aacagttatt tggagtttcg
    16801 gtcttagatt tctttgactt tcttagaaat cttgcgattt tctttcttgc tagattgctt
    16861 aaacaaaatc aagatgcgtc ctattttttg gactgtatcc acaccgattt cttcttccaa
    16921 aatttcagct acttcgtgga tgttttcatc tgtgttttgt aagagagtaa ccttgattaa
    16981 ttcacgcgca tcaagagctt gacggacgct ggttttgatt tggtcgttga gtccattttt
    17041 cccgatttgg atgataggtt tgagggtgtg tgcctggctg ttgaggaagg cacgttgttt
    17101 tgatgctaat gacataattt tcttccttgt tttttgatag ttattttagg tggtgaggga
    17161 cggtcggaac taattttcca aagatttcat ctttgaaaaa tggatttcct gacctcacaa
    17221 ttgagccttg gtctcaattg tgccccttgc caatctacag attggcaatc acaccacggc
    17281 aataactatc gctatatgag ctcactttgt tcgctcagcg attttgtttt aaataattgc
    17341 ttttcgtgtg gcgacggcga cgccttctgg tgcccagacg gcgacttttg ctgtgcctgt
    17401 tacacgaatc cagcctaggc ctgaaatgac taggtctgtc ttatctttaa tggtaaagac
    17461 atgctggact agccttggga aatcttcttt ttccttgcta tttggtggtg tcagaagagt
    17521 tcccaggtgc ttatcgtaga aagcactagc tccttcaagc ttgctacgat ggagtttgag
    17581 ttcattatca aagaaagcag taaatccttg cttttctcct gctataaagt caaagcgtcc
    17641 caaaccacct aaaaataggg tttgctcagg attaagctga taggtcttag gcttgatttc
    17701 ctttttagga ctgacatact tgaggttttt ggccgtcaag tagtgagcca tctggtggcg
    17761 gtggataatt cccggcgtat cgtaaatata agatccgtcg tcaagcggaa tctctatttt
    17821 gtccaaggtt gtccctggga agcgtgaagt agtgatgaca ttctgatcac ccgtgatttc
    17881 ttggataata gcattgatta gagttgattt tccaacgttg gtcacaccga ccacatagac
    17941 atcgcggccc ttacggtagt gttcaatctt atcaatgact tccttaatgg catgtttatt
    18001 ttgtgctgaa gttaggacca catcgactgg acgaagacct tcttcatggg cacgtttcat
    18061 gagccactgg ctaatcttac cagacttaac tgacttagga aggatatctt ttttatttcc
    18121 taccaagagg acatcattgc ccgagacgaa acgtggtaaa cctgggatga cagatccatt
    18181 aaaatcaaag atatcaatga cattgaccac taaggcatca ctgtctccca cctcgtgcaa
    18241 gagcttgagg aaatcatcgt tcgtcaactg gacatctgtg atttcattgt agtggcggag
    18301 acggaaacag cgttggcaat agacttcgcc agtctccaaa cctttttcaa gtgccgactg
    18361 gggggtaaaa ccaagaccag ctttatctgt cgtctgaatg gttgctccac aaccaataca
    18421 gagaatttct tccatagtta aattcctttt ttatatgtaa tcggtccgta cttttcagtg
    18481 atttttctca taacacgacg ctcacgagtt cggttaatct gcgttttgat tgagtcatgt
    18541 tggaccaagg gtttgactaa aattgaccga atccctgcac ggtgggctgc tcgtatatct
    18601 gtcatgagct ggtcgccaac catgaccact tcctttttgt catagtggaa ttccttcatg
    18661 gcacggtcaa taccaaatgt gaagggcttc aaggcccagt aaacgtaatc aatcccaaat
    18721 ttctcaactg ctcgttgaac gcgttttttg gtgttatttg acactacgat aatgccaata
    18781 cccgcgtccc gaaggtcatg tagccattgc ttcatctctg gcgttccatc agggttgttc
    18841 caagcaatga gggtattatc caaatcgacc aaaacagcct tgattccctg cgcctgcagg
    18901 cttgggactg tcagatcata gactgcttcc acagcaaaat ctggtatata attttcaatc
    18961 gccattctgg ctccttttct taactttttt tcgcaatttt ccttagtaac aaggataaaa
    19021 caatccacaa acctgcacta gccaaactga tatagagcca agcatggggc tcatctgtta
    19081 aaggtaaggg aacattcatt ccgaaaaagc ctgtcacgac tgccaaaaca gctagcaaga
    19141 ctgaaatgat agtcaaggtt gtcaaattgt cattcagatt attgtttaga atattgttgt
    19201 aagaggctga aagctgctgt aaaatctgag agattaggtc tgtcatggat accagctgat
    19261 gagcctcaat catggcatca tcaaactgtt ctctctcaat ctcatcaaaa ctacgataca
    19321 aggcatgacc ttgaatatgc tctaacaaaa tccgattttg tttggcagct gccgtcagat
    19381 aaaccatacc agtctccaaa tcagacaggg caaagaggtt tttcttggta gttcgctggc
    19441 gcaagaggtc attgacctca tccctactct tgtccatctg ctcaatgaca ggatagtagg
    19501 cattgctgat gatttccaga ctggcaaaga gaaacttata aatcgaaagc gtgtcatggc
    19561 tctccagata acgagtcatc tgttcaatga cataggcgtt cttggtatta ctaatggtaa
    19621 tcaggcgacg atgctcgacg ataaaggtca tgggaaaagt ctcatagtag gccttgtcct
    19681 tttttacgtc taggacatta tagataaagg taaccgtccc actttcacgg tggtagtcca
    19741 tgtgggcgcg ctcgtttcta tccagtgcgt attcaatggt ttccttgtcc aaaccgtaaa
    19801 tttcagaaag gtcttctagt ttattcaact ttcctaggtc taggtctatc caggtacaac
    19861 catggcccaa ctgcttttct aaaaccatct tttctcctta ttaaactccc tctattgtac
    19921 cacaaattac ttaaaataac aggtctagtc tgtgcgtgag aaattgctaa caactgtgat
    19981 atttcggtta gggacaaagt gaagggcttg ctcctcgcta cgaagctgtg tcgtacgaat
    20041 tcccacactg acaaccttac ccgatacagt aatcggtcca tttgtcagaa cgacctcatc
    20101 tcccacatcc agttgacgtt caaagaggat gaaaaagcca ttgatgacat cagacagaaa
    20161 gccttgggct cccataccaa tcgctacccc agcaatacca gctccagcca gcaaactaga
    20221 aactggcaaa cctaaaatcg acaaaatgca gtagagtaaa aagaaatata gcgtataatt
    20281 aaacacattt tctagtaaac gtgagatggt tttttggcgt ccgacatcat gacgagacat
    20341 ttttagagaa ggtttgacaa ttctctgcac catggtatga agcatttttt tagcaatata
    20401 aaatacaatc aaaagcagta aaagagaaat gaccttggtt agaatattct cgataatggt
    20461 tgtcacatct agcttttcaa tataagcctg aataaatttt tgcatataaa actcctttat
    20521 ttcattatac catattttca tcagtctcat tctccataaa tattcaaaaa atagacagaa
    20581 aattcttgat tttgtctatc atttatacta taatcataat cactacacaa aaaggagatt
    20641 attatgaaaa gaatcattca tgcttggaat aaggcaagcc ttgtcaagcg tatcttgatt
    20701 ggtatgcttt gtggggaatc ctaggactga cccttcctaa tctctcaggg attgggctac
    20761 tcggggattt attcgttgga ggtctaaaag ctgttgctcc tatcctagtc tttgctctcg
    20821 ttgccaatgc cctttcccaa catcaaaagg gacaagatag caatatgaaa actgttgttt
    20881 tcttgtatat cctatgatag ggactttcgc cgctgctctt gtagctgtac tagcaagttt
    20941 catcgtccct attgaaatta ccctaaatag tgccaatact gaaattgcac caccagatgg
    21001 gattgggcag gtcctcagca acctcttgct caaactggtt gacagcccag tcaacgccct
    21061 gcttactgct aactatattg gaatcttatc ttgggcagtc atttttggaa tcgctatgag
    21121 agaagccagt aaaaatagta aagaattgct aaaaactatc gctgacgtga cttctaaaat
    21181 tgtcgaatgg atcatcaatc tgactccatt tggaatcctt ggtcttgttt ttaaaaccat
    21241 ttctgacaag ggagtcggaa ggcttgccaa ctacggtatt ttattggttc tattagtaac
    21301 gactatgctt tttgttgccc ctgtggtcaa ccctttgatt gccttcttct ttatgagacg
    21361 caatccttac cctctagttt ggaactgcct ccgtgtcagc ggtgtgacag cctttttcac
    21421 tcgtagttct gcggctaaca ttcctgtcaa tatgaaactc tgccatgacc ttggactcaa
    21481 cccagatacc tattctgttt ctatcccact cggttctact atcaatatgg ctggagtagc
    21541 gattaccatt aaccttttga cccttgttac agttaacact cttggaattc ctgttgactt
    21601 tgccacagcc tttgtcctca gtgtggtagc agctatctca gcctgtggtg cttcaggtat
    21661 tgccggaggt tccctccttc ttatcccagt tgcttgtagc cttttcggta tttctaacga
    21721 tattgccata caaattgttg gggttggttt tgtgattggt gtcatccaag actcatgtga
    21781 aacagccctt aactcttcta cagatgtcct ctttaccgcc gttgccgaat acgcagcaac
    21841 ccgtaaaaaa taactcatca aggcaagcct gctcatgtct tgtcttttac gcttttattc
    21901 taacttatta ggaaattctt atgtctatta gccaacgtac gaccaagctc atcttagcta
    21961 cctgtcttgc ctgcctgttt gcttattttc tcaatctttc gtcagcagtt tcggctggaa
    22021 ttatcgctct cttgagccta tctgatacgc gtagaagtac tttaaaactg gctcgcaatc
    22081 gtcttttttc tatgcttcta gctctggcta tcggtgttct agcttttcac ttgagcggat
    22141 ttcatatctg gagtctcggc ctctatctgg ccttctacgt tcctttagcc tacaagatgg
    22201 gctgggaaat tggcatcaca ccaagcacgg ttttggttag ccatctcttg gttcaagagt
    22261 caacctctcc agacctccta gtcaacgaat tccttctctt tgctatcggt acaggatttg
    22321 ccttgcttgt taatctctat atgccttcac gagaagagga aatccagcac taccacacgc
    22381 tggtggaaga aaagttaaaa gatatcctcc agcgcttcaa atactattta tccagaggag
    22441 acggacgcaa ccgagcacag ctggtagcag aattagacac gcttttgaaa gaagccctca
    22501 gactggtcta tttggatcac tctgaccacc tctttcacca gaccgactac catatccact
    22561 actttgagat gagacaacga caaagtcgta tcctgagaaa catggcccaa cagattaaca
    22621 cttgtcacct tgccgccagt gaaagcctga tcttagcgca actcttttca aaaattgcag
    22681 gtcaactgag ccagaccaat cctgcttctg atttgctaga tgaaattgaa cgttatctgg
    22741 aagtcttccg gaaccgcagt ctgcccaaga caagagaaga atttgaaacc cgcgccaccc
    22801 ttcttcaact cctacgtgaa gccaaaacct tcatccaagt aaaagttgat ttttaccaaa
    22861 aatatagaca gtaatactct tcaaaccacg tcagcgtcgc cttgctgtat atatgttact
    22921 gacttcgtca gttctatcca caacctcaaa acgatgtttt gagctgactt cgtcagttct
    22981 at
//
LOCUS       DAVBCN010000026        22861 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVBCN010000026 DAVBCN010000000
VERSION     DAVBCN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22861
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.026"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(<1..337)
                     /locus_tag="VQ497_000962"
     CDS             complement(<1..337)
                     /locus_tag="VQ497_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002202361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2517288.1"
                     /translation="MDSASIIAHTIHQKFNLKVPNYRQEEDWARMGLPITRKEIANWH
                     IKASQYYLESLYNLLREKLLEQPLLHADETSYRVLESDSQLTYYWTFLSGKSEKQGIT
                     LYHHDQCRSG"
     gene            complement(345..650)
                     /locus_tag="VQ497_000963"
     CDS             complement(345..650)
                     /locus_tag="VQ497_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HET2517289.1"
                     /translation="MFPVETEEITYKRKKSKGKRQALLAQFDSEEVHHQVEESICSDC
                     QGDLKEIGATLQRQELVFIPAQLKRIDHIQHAYKCQACSDKNPSDKIVKAPIPKAPL"
     gene            complement(637..831)
                     /locus_tag="VQ497_000964"
     CDS             complement(637..831)
                     /locus_tag="VQ497_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2517290.1"
                     /translation="MKIIQQQSATIDSLTNALALLREQVAYLTQKLYGKSSEKSVCPS
                     GQLNLFEEESPSEKDGDVPS"
     gene            complement(828..1085)
                     /gene="tnpB"
                     /locus_tag="VQ497_000965"
     CDS             complement(828..1085)
                     /gene="tnpB"
                     /locus_tag="VQ497_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET2517291.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKYRFKALYWDGQGFWLLFSIIKVENKEGKPMEED"
     gene            complement(1066..1251)
                     /locus_tag="VQ497_000966"
     CDS             complement(1066..1251)
                     /locus_tag="VQ497_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517292.1"
                     /translation="MPQPIVPVEIPQSRPFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
     gene            complement(1555..3117)
                     /gene="guaA"
                     /locus_tag="VQ497_000967"
     CDS             complement(1555..3117)
                     /gene="guaA"
                     /locus_tag="VQ497_000967"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET2517293.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            3259..3957
                     /locus_tag="VQ497_000968"
     CDS             3259..3957
                     /locus_tag="VQ497_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2517294.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(4000..4896)
                     /locus_tag="VQ497_000969"
     CDS             complement(4000..4896)
                     /locus_tag="VQ497_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET2517295.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLLIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(4905..5840)
                     /locus_tag="VQ497_000970"
     CDS             complement(4905..5840)
                     /locus_tag="VQ497_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HET2517296.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(5837..6562)
                     /locus_tag="VQ497_000971"
     CDS             complement(5837..6562)
                     /locus_tag="VQ497_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HET2517297.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(6646..7281)
                     /locus_tag="VQ497_000972"
     CDS             complement(6646..7281)
                     /locus_tag="VQ497_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HET2517298.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(7283..8110)
                     /locus_tag="VQ497_000973"
     CDS             complement(7283..8110)
                     /locus_tag="VQ497_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2517299.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(8132..8468)
                     /locus_tag="VQ497_000974"
                     /pseudo
     CDS             complement(8132..8468)
                     /locus_tag="VQ497_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
     gene            complement(8469..9185)
                     /locus_tag="VQ497_000975"
     CDS             complement(8469..9185)
                     /locus_tag="VQ497_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET2517300.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNHILDSQIEGVISMADII
                     EKAGITPIGVYTHEPRDQAPLVIKEVNAIKVALLAYSYGFNGIEQYIFQEDYNRYLSD
                     LNEDKIKAEVERAEKEADITIIMPQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSKTVEKDGDKKLIIY"
     gene            9708..10319
                     /locus_tag="VQ497_000976"
     CDS             9708..10319
                     /locus_tag="VQ497_000976"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET2517301.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEGIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            10364..11092
                     /gene="rlmB"
                     /locus_tag="VQ497_000977"
     CDS             10364..11092
                     /gene="rlmB"
                     /locus_tag="VQ497_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET2517302.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFLNRL"
     gene            complement(11131..12042)
                     /gene="trxB"
                     /locus_tag="VQ497_000978"
     CDS             complement(11131..12042)
                     /gene="trxB"
                     /locus_tag="VQ497_000978"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET2517303.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVKEIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(12108..12332)
                     /locus_tag="VQ497_000979"
     CDS             complement(12108..12332)
                     /locus_tag="VQ497_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HET2517304.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(12410..13153)
                     /locus_tag="VQ497_000980"
     CDS             complement(12410..13153)
                     /locus_tag="VQ497_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2517305.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILLLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(13153..13953)
                     /locus_tag="VQ497_000981"
     CDS             complement(13153..13953)
                     /locus_tag="VQ497_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2517306.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(14111..14467)
                     /locus_tag="VQ497_000982"
     CDS             complement(14111..14467)
                     /locus_tag="VQ497_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET2517307.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(14480..14992)
                     /locus_tag="VQ497_000983"
     CDS             complement(14480..14992)
                     /locus_tag="VQ497_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET2517308.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(14992..15486)
                     /locus_tag="VQ497_000984"
     CDS             complement(14992..15486)
                     /locus_tag="VQ497_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2517309.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            15621..16067
                     /locus_tag="VQ497_000985"
     CDS             15621..16067
                     /locus_tag="VQ497_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HET2517310.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            16064..16711
                     /locus_tag="VQ497_000986"
     CDS             16064..16711
                     /locus_tag="VQ497_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HET2517311.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(16732..17313)
                     /gene="pdxT"
                     /locus_tag="VQ497_000987"
     CDS             complement(16732..17313)
                     /gene="pdxT"
                     /locus_tag="VQ497_000987"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HET2517312.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(17314..18189)
                     /gene="pdxS"
                     /locus_tag="VQ497_000988"
     CDS             complement(17314..18189)
                     /gene="pdxS"
                     /locus_tag="VQ497_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HET2517313.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(18341..19720)
                     /locus_tag="VQ497_000989"
     CDS             complement(18341..19720)
                     /locus_tag="VQ497_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET2517314.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKDKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            20015..20938
                     /locus_tag="VQ497_000990"
     CDS             20015..20938
                     /locus_tag="VQ497_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET2517315.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGISPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            20998..21603
                     /locus_tag="VQ497_000991"
     CDS             20998..21603
                     /locus_tag="VQ497_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2517316.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKTIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            21621..>22861
                     /locus_tag="VQ497_000992"
     CDS             21621..>22861
                     /locus_tag="VQ497_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2517317.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRNFKFKFELEVLEIDEVPMF
                     NQDEKWDESFQLRFLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGAS"
BASE COUNT         6801 a         5127 c         4165 g         6768 t
ORIGIN      
        1 aaccacttcg acactgatca tggtggtaaa gcgtaatccc ttgtttctct gatttacctg
       61 acaaaaaagt ccaatagtaa gtcagctgac tatcactttc taagacccga taagaggttt
      121 catctgcatg aagaagaggt tgttctaaca acttttctcg taaaaggtta taaagggact
      181 ctaaatagta ttgactcgcc ttgatatgcc aattagcaat ttccttacgt gtgattggta
      241 aacccatcct agcccaatct tcttcttggc gataattagg taccttcaga ttaaacttct
      301 gatggatggt gtgagcgata atagaagctg agtcaatagt ctcgttataa aggggcttta
      361 ggaataggag ctttcacaat tttatcactc ggatttttat cactgcatgc ttggcatttg
      421 taagcgtgtt ggatatgatc tatgcgtttt aattgcgcag gaataaagac taattcttgt
      481 cgttgaaggg ttgctccaat ctcttttaga tctccctgac aatcagagca aatgctctct
      541 tctacttgat gatgaacttc ttctgaatca aattgggcaa gaagagcttg gcgtttccct
      601 ttagatttct tacgtttgta ggtaatttcc tctgtttcaa ctgggaacat ctccatcttt
      661 ctcagatgga gactcttcct caaaaaggtt gagttgtcca gaggggcaaa cacttttctc
      721 agaggatttt ccatagagct tttgagtcag ataagccact tgttcacgaa gaagggcaag
      781 tgcattggtg agactatcaa ttgtagcact ctgttgttga atgattttca atcttcttcc
      841 atgggcttcc cctccttgtt ttctaccttt attatactaa atagtagcca aaatccttga
      901 ccatcccagt aaagggcttt aaagcggtat ttacgtccac cacaaaagag aaaaacttga
      961 cctgagaaag gatctaattc aaagtgggtt ttaaccagat aagccagtga atcaatgcct
     1021 tgcctcatat ccgttttccc acatacgaga tagacctgcc ctaggctaga tagatgaatt
     1081 gtcatagagc aacaccttat ccaaaagctg ttctaccatt tcgagattga gagattgaaa
     1141 aaaacttact tcaagcttgc caatacgaat tttaagcaga atatcatttc tctttttaga
     1201 atcaaaagga cgagattgtg gaatctctac aggaacaata ggttgtggca ttaaaatatc
     1261 ctccaatagt tttactttac taatagtata caggaggaac tccatgaatg atagatacct
     1321 tgttatgacg cgattactat attagaaagt tggctctcta attaaattaa ttctcattat
     1381 atatatcaaa agaccgcagc ttgaaaacta cggtctaatt ttttgtccaa atgtaaacca
     1441 cttggaacta ttattcaatt tattcttttt agtaaatgtt cccgttttaa tcccagttac
     1501 agtttttaat cctctgtaac cgttgatttt acaaagcttt taattttttt gcgattattc
     1561 ccactcaact gttgcaggtg gtttacttgt aatatcgtag acgatacggt taacatgatc
     1621 cacttcattt acgatacgta ctgagatttt ttgaagtact tcccatggaa ttttggcaaa
     1681 atcagcagtc ataccatcga tagaagtgat agcacggatt gcaatcgtgt agtcatatgt
     1741 acgaccgtca cccataacac cgactgaacg aacgcctgtg ttaacagtga agtattgcca
     1801 aatatcgcgg tcaagtccag ctttagcgat ttcttcacga agaatagcgt ctgattcacg
     1861 aacggtttca agtttctctt cagtgatttc acccatgaca cggatagcaa gtcctggtcc
     1921 tgggaatggt tggcgccata cgatatggtc tggcatacca agctctgtac caagagcacg
     1981 aacttcatcc ttgtaaagag tattgagtgg ttcaatcaat tcaaactgca tatcttctgg
     2041 aagaccaccc acgttgtggt gtgacttgat agtttgagct gtatccgtac cagactcgat
     2101 aacatctgta tataaagtac cttgagcaag gaatttcaca tctttgagct tgcttgcttc
     2161 gtcatcgaat acatagacaa actcgttacc gatgatttta cgtttttgtt caggatcaga
     2221 aacgccagca agtttgtcaa ggaaacgttt agcagcgtct gctttgacga tattcaaacc
     2281 aaacttacca ccgagcatgt ccataacttg atcagcttcg cctttacgaa gaagaccgtg
     2341 gtctacgaag atacagatca attgatcgcc aatcgctttt tggagaagaa ccccaacgac
     2401 agatgagtca acaccacctg atagaccaag aaggacacgt ttatcaccga cggtttcacg
     2461 aatttttttg atctgcatgt caatgaaatt atccattgac cagtcacctt tagccttaca
     2521 aatgttaagg gcaaagttac gaaggatatc atttccgtat acagaatgac gaacttctgg
     2581 gtggaattgg ataccgtaaa tgtgtttatc tgggttttcg atggctgcgt atgggcagtc
     2641 agctgatgta cctgtacgaa caaagtcagc aggaatctca gtaaccgcat caccatggct
     2701 catcaaaaca gtctgttcat caggtgttga ttcaaaaagc gctgatggtg tgtgagttag
     2761 ggttgattga ccgtattcac gatttccagc atcacctgca ggaacaactt ttcctccaag
     2821 tttatgggtc aataactgca taccataaca gattcccaaa attggaattc cgagttcgaa
     2881 gatttctggg tcaatatcaa atgaaccatc ttcatataca gaatttggac cacctgatag
     2941 aataattcct acaggattga cttcacgaac ttcagcagct gaaattttat ggctttttag
     3001 ttctgaaaaa acaccaatct cacggatacg gcgtgaaatc agctggttgt actggctacc
     3061 atagtccaat acgatgattt tttctacatc ttgcaaatca gttgaaatgt tgctcatctt
     3121 tttcctttct caaaagaaat atcttttctc aatattctat cacaaataag ggcgaattac
     3181 caactaaaca aggcgaagtt tgactttttc tagtttattg gggtacaata gagaaaagat
     3241 agaaatgaag tgaaaaatat gctacctgct tatatgaaaa tccatgatca gataaaaaag
     3301 gatattgacg agcaccgttg ggctattggt gagaggcttc ccagtgaaag agatttagct
     3361 gagcagtttg cggtcagtcg catgaccctc cgccaagccg tatctctatt agtcgaagaa
     3421 ggcgtcttag agcgccgtgt aggaagcggc acctttgttt ccagtactcg agtacaagaa
     3481 aagatgcgag ggacaaccag ttttactgaa attgtcaaat cccaaggtaa agttccctct
     3541 agccagctca tttcctacaa aaaaaccatt cccaatgagc aagaagttgc caagctagga
     3601 atttttccaa cggacaatat tatccgaatg gagcgggtcc gctatgccga ccaagttccc
     3661 ctagtttatg aagttgcttc tattcctgaa aaattcatta aggactttaa aaaagaagaa
     3721 atcacaagtc atttcttcca aaccttgcaa aaacatggct atcgtatcgg caaatctcaa
     3781 cagaccatct atgctcgcct tgctaaagaa aagattgccc actatttgga agttgaaaaa
     3841 ggacatgcta ttcttggatt gacccaggtt tcctacctag aagatggtac tgcttttgaa
     3901 tacgtaaaaa gtcaatatgt aggcgaacgc tttgaatttt atcttgaaaa taattagatt
     3961 accacgcaaa aactctctgt cacggacaaa gagtcttttt tatttttcta agaataaatc
     4021 tttcaaagaa atcaaagtta cagcaaacaa tatcattgcc ataccaagaa aatcaatggg
     4081 ataaaattgt tcattcatta ttaagaaggc aaagaaaatc gccgatattg gctcaattga
     4141 agccaacaag cttgacttga ccggtcctat cagactggct cctttaagga aagctgtata
     4201 ggcaaagaca gtcccgataa ggataatgcc tgcaaacgca aggagaaaat caagactagt
     4261 cgggatatcg gcctgtagaa cccctgtaaa aggaagggcg accaaacctg ctatgaccat
     4321 tcccacacca atgagcaagc tgctccccca ctttttaatc aaggctatgg gtaaaatgat
     4381 atacagagca taagtcaagg cagaaaagag accccagaac agaccagcag gtgtcatgga
     4441 taactggtcc aactgcccat gtgttgcgat caggaaggtt cctccgatgg cgaatatgat
     4501 ggaaactatc tctcccagtg tcggtgccac cctatccttg atacagctat aaattaaaat
     4561 tccgacagga caaacatact gaagcaccgt cgctgttccc gcattggtct cctgaatagc
     4621 agacagatag gcgaattggt tgagaaaaag accaatcaga gcaaaaataa gaagagacag
     4681 caaactcttt ctatccttta aaaagaccag tattttatcc tttgcagtag cataagccaa
     4741 gagcatgaga attccaccag cgattaaaag acgcaagttg gtcaagacca gagccgaaat
     4801 tccgtgtgcc attaggtatt ggccactcgt tcctgacaac ccccaagcaa taccagccac
     4861 aactgttagt aaagtccctt ttaaactatt tgacatgtct ctccttactc ttctttgcga
     4921 atcaagttca tcacttgatt gcggtctaat atccattgag ctctctcgtc cttttcatag
     4981 gtacgattcg ataggaaaat agtggcttct tgcttctgac gattccacat gataaaggta
     5041 cctgtatagc ccgtatggtc tagccaatct ccttccaaat tccatgctaa agaacgttcc
     5101 ttgtcatcca aaggagaaaa attttgattt aagtctcttg caaaatcatc tgctaaatag
     5161 tgttctaaaa agatttgtaa atcctttata gtcgaaaata aaccagcact cccagcatgt
     5221 ctacccagga gacgagcctt gggatcatgc actatgcctg cctctacacc tctaactgtt
     5281 ggaacagcaa gctcaactgg cccaaacttg gtttccgtca ttccccaagg tttccagact
     5341 tgatccttta aaatcacatc caaatcttga ttaaaaattc tttccaaaat aaagcccaac
     5401 agcaaaaaat ggacatccga ataaagaaag gctggctgac ttcgtctgtt gagatgaaac
     5461 atcgcttcct ttaattcagg ggctgttaaa agatcacgat taggaataaa aggatcaagg
     5521 tctgttgcat gagtcaagag ctggcgaata gtgatgtctg gataatcact ctcaggtaaa
     5581 aaatctatta ccagtctatc aatatctaat tgacctattt cccacaagaa ggtacaaact
     5641 gtgccaaccc caacaacctt gctgacacta gctagatcat aaactagtcc tgcctcagtc
     5701 tgcaagccat gctctgggtc actctggcct aaatagaact ctgtccattg attgtcctta
     5761 aaatacgcaa aagaggctcc gggataaatt cctgcctcga tttgttcttc tattttttta
     5821 ataatcttgg tccacttcat acttcttcaa accacaattc aacattattt ctatctttac
     5881 caaggaagaa tttttcagac ttaggaataa aatattcagt agactcaaac ttctggcgaa
     5941 gacttgctat gtctaattca ttgaccaaga acttgagcat agataagtcc caggtaaccg
     6001 tattgtccac agtcaaatcc tgcccctgag ctggaataaa atcaagggat gccccaattt
     6061 cagatgattc caagaaactt tcgatatcag ttgggagatg taattccata gaaatctcaa
     6121 atttacttaa agaaattgat gccaaatctg tttgaaattc aggcttttct cctacttcta
     6181 ctagacttgc tctgtcatct tccgcatgaa tcaaaatcaa atcatcttct ggtgagaaaa
     6241 tttcaaaagc gtagccattt tgacctttat ataatcgatg aatcgaatct gttttagata
     6301 agattccttc aatttctaag ggattttcca ccttgacaat caatctagct agtttttttc
     6361 ttccctctac cttacgagta cgcatactgg gagcttcttc taaaaccagc ttttcaagac
     6421 ccgtttggtc acctagtgac agaaaggccg attcttctaa caaggccttc attccaaggg
     6481 tttcaatata aaatgtttca tttaattttc tattattagc ttttaaagta ggaataatcc
     6541 gtacaatctg atttacattc ataaattcct ccgtcacttt ttattttaaa ggattttaga
     6601 attttttaca agatattatg ctcaaattta acattattta gctatttagt aaagatagtc
     6661 tttcaaggat tttgacaaag cctgataacc agcaatactg aggtgcagtc catcagttgt
     6721 atattctttt ttgagttggc ctgcttggtc tgtcaaacaa tcaaatactg gcacaaattc
     6781 cacctgcata taggcagatg caagctcttg ataggcttga ttccagttct gaattttttc
     6841 attcgagcgg atatagactg cctgctggta ctcctctccc tcattgacag gcaaaatgga
     6901 aagcaattta atctctgtca atggataatc gcgagcaacg gattgaatga tagcttcgag
     6961 attattgaga gcctcattca caggaacatc ctttccgata tcatttgtcc caatcagaag
     7021 aaaaatttta tctactgctc caccatatag atgagcatca agattctcta gtaacagtcc
     7081 tgtctgatag ccacgaattc ctcgattgac aatcgtcttt gaagtcccaa atagctcctg
     7141 tagaggataa tactcgacaa tggaatcccc aataaaaaga atgtttggtt ctacaacaga
     7201 aatgtgattt aggtgacgat acttagtttg aattttttct tgttccttta ggagccaatt
     7261 ttctaataac tgtactgcca cttcattctc ctttttctaa ccagttttcc aagacttgat
     7321 aaactcctcc ttggctgttg gctggtgcta gagcagtcgc cacagctttg gctttctcat
     7381 cagcattttc catcgcatag gcaattccag ccatttcaag catttcaaca tcattttcac
     7441 tatcaccaaa agccatgatt tcttgggatt tcaagtccca gcgcttgagt aattcctcca
     7501 agccccatgc tttatgaatc ccagcttgga ggatatcaat gcaaccatag ccactggata
     7561 cagctcggac acggccatca aagagagcat tgatttcttc caaaaccgaa ctcaaacgtt
     7621 cctcaccaac aaccatgctc atcttgagca caccaccaaa gaggtcagat gttaattcat
     7681 ccacaaattg catccgttgg tagaattttt caatcatttc tggagtcata aaactttcaa
     7741 gatctgtaaa aatcgtacct tccttgacaa aatcaccctt catccccgtt acaacaaact
     7801 ggtcctgaca cgctcgaccc ttgaaatgag ccaaagcctt gttgacaatg gcgtcatccc
     7861 atgtctgagc ctgaatcaat tcattgtttt caaaaatacg agcgccatta gcaacaacca
     7921 gaaccactcg atccaccaag ggactcagta gttgtctcat gcggtgaatt tcattgcccg
     7981 tcgcaatgac aaaacgaatg cccctttgat ccaactgatc taaaatcttt tccaatcgtg
     8041 ggagatcaag ctgaccccta gcatccagca aggtcccatc catatctgtc gcaattacct
     8101 taatcatcat ttactttcct ctggattcaa gctaatacca cttcaatccc acatgttcat
     8161 tcatttcttt ataggctgta tcaattcgtt ccttagtcgc ttcatctaac tggtcacgat
     8221 gactgccacc cctctataaa atcttccaaa atataagttt ggtaatgata caagggatat
     8281 ccttctggtg aaaaggttcc ctttggtgtt cgattgaccc aagtaggatg agctttagct
     8341 gttccgatag ttgtttttcc atccttcttc ttgatggtga catccatgag aacaccacgt
     8401 tcagtccact tagcattctc ttcatctccc atagattcaa tccgttgatt ggaaatgaag
     8461 ttccccattt aatagataat gagtttctta tctccatctt tttcaaccgt tttagatggt
     8521 tcaacaacgt gaggatgccc tccaaagata atatccgctc cccaatcgat catcttgtga
     8581 taaagagctt tttgttcttc agttggttcc aatcgatact caacacccat ctgaggcatg
     8641 ataatggtga tatctgcttc cttctctgcc cgttcgactt cagcctttat cttatcttcg
     8701 tttaaatctg aaagataacg attatagtct tcctgaaaaa tatactgctc aattccattg
     8761 aaaccatagg aataggctaa caatgcaacc ttgatagcat tcacttcctt aatgaccagc
     8821 ggagcctgat cacgtggttc gtgcgtataa actccgattg gagtgattcc agctttctca
     8881 ataatatcgg ccattgaaat aactccctca atttgcgaat ccaaaatatg attatgagct
     8941 aaatccagca catgataacc tgcttcctta atagcatcca taacttcaac aggagcatta
     9001 aagagaagat aacccgctaa ataatgatcc ttattaatgg ttccttcaaa atcaccaata
     9061 gctaaatctg cttgcttgag ccaaggagtc acatactcaa aattttcatg aaagtcatag
     9121 gtaccgtctt cttttttagc tgaaaagaaa agtccatcgt ggtagagcaa atccccgtgg
     9181 gccataattc tggcagtttt ttcctcttcc tactcctgag acttttgctt agtcccctct
     9241 tgagaaatag tatctcgttt ttgactagtc aagggattcc ttggaagctt tcaaacaaca
     9301 agaccaagcc cattgataaa ccaactgcta gcaagagtat cgccacaaat cccttattgc
     9361 tccacttgcg ataactccta aaaagtttac caagcccttc ataaaacgaa aagctaaacc
     9421 accctgattt cgattttgtc ttctttgtat cttcattctc cctactttct tatgcaagcc
     9481 ttttcttttt attatatcac agataagtat ttctttcaca attgaattga acttcccatc
     9541 tattttctat aaatcctaaa tgccataatg ctttcaattc ttgtcatttt gtgatatcat
     9601 gtagaggtaa tgaaaggaaa tgaactaatc cacagtggct tattccaagt ataccacttg
     9661 ggctttggca gtagttaact gcactaaaca taatataagg agagaaaatg tctgcaatag
     9721 aacgtattac aaaagctgct cacttaattg atatgaacga tattatccgt gaagggaatc
     9781 ctactctacg cgcgattgct gaggaagtca ctttccccct atctgaccag gaaatcatcc
     9841 taggcgaaaa gatgatgcaa ttccttaaac attcccaaga tcctgtcatg gctgaaaaaa
     9901 tgggactccg cggtggtgtt ggactggctg ctccccagtt agatatctca aaacgcatta
     9961 tcgctgtttt ggtacctaat attgttgaag aaggcgaaac tccacaggaa gcctacgatt
    10021 tggaaggcat tatgtacaat ccaaaaatcg tctctcactc tgttcaagat gctgctcttg
    10081 gcgaaggaga aggttgcctg tctgttgacc gtaacgtgcc tggctatgtt gttcgccatg
    10141 cccgcgttac tgttgactac tttgacaaag atggagaaaa acaccgtatc aaactcaaag
    10201 gctacaactc cattgttgtt cagcatgaaa ttgaccacat taacggtatc atgttttacg
    10261 atcgcatcaa tgaaaaagac ccatttgcag ttaaagatgg tttactgatt cttgaataaa
    10321 gaaaatcccg ttgcaagacg gggttttgtg ttataataga ggcatgaaaa caaatgatat
    10381 tgtctatggt gtccacgccg ttaccgaagc cctccttgca aatacaggaa acaaactcta
    10441 cctccaagaa gatctccgag gtaagaatgt tgagaaagtc aaggaactag ctacagaaaa
    10501 gaaggtgtcc atttcttgga catcaaaaaa atctctctct gagatgactg aaggtgctgt
    10561 tcatcaaggt tttgttctac gagtgtctga atttgcctat agcgagctag attacatcct
    10621 tgcaaaaaca cgccaagaag aaaatccact tctattgatt ctagatggtc taaccgatcc
    10681 ccataatctg ggttctatct tgcgaacagc cgatgcgacc aatgtttcag gtgtcatcat
    10741 tcccaagcac cgtgctgtcg gagtaactcc tgtcgttgcc aaaacagcca caggtgctat
    10801 tgaacacgtt ccaattgccc gagtgacaaa cctcagtcaa accttagata aactcaagga
    10861 tgaaggcttc tggacctttg gaacggatat gaacggtact ccttgccaca agtggaatac
    10921 aaaagggaaa atcgccctca tcatcggaaa tgaaggaaaa ggcatctcta gcaacatcaa
    10981 aaaacaggtc gatgaaatga ttaccattcc gatgaatgga catgttcaaa gcctcaatgc
    11041 cagtgttgct gcagccattc tcatgtacga agtttttcta aatagactat aaaaaagttt
    11101 ccagtcatct gattggaaac ttttttatga ttaactatgt tctgtgataa acttgtaggc
    11161 ttcttgacca gcgatagctc catctccaac tgctgttgtt acttggcgaa ggtctttcaa
    11221 gcgaacatct ccaactgcaa agataccgtc gactgcagtc ttcatgtggc tatctgtcac
    11281 aatccagcct gcctgatctt ggatattcaa ttctttaaca aaatcgctaa gagggtccaa
    11341 accaacatag ataaagacac caccgaaggc ttgctctgtc acttgacctg ttttcacatt
    11401 ttcaaaaacg acagattcta ctctgttttc acccttgatt tccttcacta cagaatccca
    11461 gataaagctg attttttcat tcgcaaaggc gcgatcttgt aaaacctttt gggcacgaag
    11521 ttggtcacga cggtgaacaa tggtaacagt cttagcaaaa cgagtcaaga agagggcttc
    11581 ttcaacagct gaatctccac caccaactac caataaatct tggtcacgga agaaagcacc
    11641 atcacacaca gcacagtaag aaacaccacg actgttcagt tcttcttctc caggcactcc
    11701 caaaggacgg tgtttagaac cagttgctac gataactgta cgtgtttcat atgtttggtc
    11761 atcagtcatc actttcttaa aatcaccatg gtcttcgaca ttttcaacat aaccataaat
    11821 gtgctcaaca ccaagatttt caagtggttc aaacatcttt tcagccaatt caggtccact
    11881 aatattagcg tatcctgggt aattttcgat atcagatgta ttattcatct gaccacctgg
    11941 cagaccacct tcaatcaaag ctacttttag attgcttcga gcagcataca aggccgcagt
    12001 catccctgca ggtccagcac cgataataat agtatcgtac atatagattc cttctttctt
    12061 gttgtaacta tctttattct aactctttct tgtcaatcac gcaaggatta tgccttgaaa
    12121 acaagaatta aactcataac cgcaaaggat aatactggga caaccaagac tccaatcata
    12181 atcatatcat agagatgggc ttggcgagcc ctggctgtct tatcaactcc cgacatggct
    12241 ctcagaccaa tccaaatccc taaaaaaatc agaacaagga tggtggtcaa gatcaaactc
    12301 tcgaaatata aagaaaatag ttgcagtagc atgatttctc tcatttctat cttttttaaa
    12361 gagtaaactc agctagtcca actaactgag ttttccttta tctattatat caaatataag
    12421 tccgtttgta actagcgaag aattcttttg tccgctcttc tttaggggtg tggataatct
    12481 catccggagt tccagactcg atgattttcc ccttatctaa taagagaatt ttatccgcaa
    12541 cttgggctac aaaggacatg tcatgactga ccaaaatcat ggtctgacct gacttagcag
    12601 catctgcaat agacttttct acttcaccga ccaattctgg gtcaagggct gaagttggtt
    12661 cgtctaagag caaaacatct ggtttcatag caagcgcacg cgctagggca acccgttgct
    12721 tctgtccacc tgataaatgg cgaggataat ggttttcacg gtccgaaagc ccaaccttag
    12781 ccaactcttc cttggcaatc ttagtcgctt cttggtcaga taatttcttg acaacaacca
    12841 agccttcttt cacattatca agtgctgttc gacgttcaaa taaattaaac tgttggaaaa
    12901 ccatagacaa cttacggcgt agggcaagga tttcttcttg agtgatttta gaaaaatcaa
    12961 ctgaaaaacc atcaatctga atagagccac tgtcaggtgt ttcaagataa ttgagactgc
    13021 gaagaaaggt tgattttcca gctcctgaag aaccaatcaa ggctacaact tccccttttt
    13081 gaatatccaa gttcagatga tccaagacag tctgtcctga aaaggatttg cttaaattcg
    13141 aaatcttaat cattaacgaa ggtctccttt cacatctgtt tgcactgtat caggtgcaga
    13201 aatagccatt tttctctcga tgaaacgacc gaggctttca attccgatat tgactaccca
    13261 ataaacaagg gcaacagaga tgaagcgttc aaaatagcga taatcagctc cacctagaat
    13321 ctgagcttgg gcaaagactt ccacaacacc cgcactaaaa gctagagatg ttcccttggt
    13381 caaaccgatg agggaattaa tcaaggttgg agtagctacc accgctgcat taggaataat
    13441 cactcgtcga taaacttgcg ctcgggtcat acccagactg cgcgccgcct caatctcacc
    13501 aggattaact gagagaatgg ctgcacgaat ggtttcacta gcataagctg cctcattaaa
    13561 ggcaaaagcg acaatcgcaa aagctgcagc tggaatcgca ttgatattga gaccagttcc
    13621 ccattgctga ttgagggctt tcaaagccaa agggattccg tagtaggtca acatgagttg
    13681 caccaaaatc ggtgtccctt ttaagaaact aacaaagaag gcctgcaagg gatataaaat
    13741 cttgacacga ttgatcttca caatggcaaa aagaagcgcc aaaaccaagc caaaaagggc
    13801 accaccaatt gtcaacataa ttgttgttgg aagttgttgg acaattctag ggattccatc
    13861 aaagaccgaa cgtaggctaa acagcttgcc atccggaatc aattgcatca agttttggta
    13921 ccaatctgat gctaaaatcg ttgtaacatt cataaaaaca tcctcttctg ataatgattc
    13981 attctaccat gcttctgccg tcaatgaaat tatttgctac ggtcagatac agactttgtc
    14041 tacctactag tttatcatac tttgaaacag ggaagttata tattttatct atcaaagaga
    14101 tagataaaaa ctatttcagt cccagttctt cataagattt tcgataaccg atttgcttaa
    14161 cagttccatt ttctactaaa atgggccgtt ttaacaacat accgtcactt gctagcaact
    14221 cagccgcttc ttggtttgac aaacttccta ccttatcttt tagccctaat tcacggtatt
    14281 tgataccact ggtgttgaaa aattgcttca attcaaatcc tgaggtttct agccaattca
    14341 aaatgacttc ttggctaggt gtttcttcca cgatatggac ggctttatag tccacaccta
    14401 attgatttaa ttcttgtttt gcttttttac aagttgaaca tttggggtat tcgataaatt
    14461 ctaacatgtg cttctccttc tatttgttta tatctttaaa atctacgcct tcatgctcca
    14521 agagccaagc tttcttttcc actcctgcag catagcctgt cagacgcttg cctgctccca
    14581 acacacgatg acaaggtact aggatagacc aaggattgcg tcccactgct ccaccaattg
    14641 cttgagcaga agccacttgc aggtcttgag caatttgtcc ataggtcact gtctgaccat
    14701 aaggaatgcc ctgtaaatag tcccaaactc tcttttcaaa attcgttccg attggcgcca
    14761 agagcaagtt ggataaatcc tgaggcttgc ctttaaagta atcatctaag caagcaataa
    14821 ctgggtctaa aataggatga ctaacaactt cttctatcgt ttcatctcct agtcccctct
    14881 caaaatgctt ctgctcctga acccaaattc catacaaata atggtcatca gctacaagtg
    14941 atagaatacc aattggtgag gagtagagta tttttacata cttcttttgc attatttctt
    15001 taatttttga taggccaagc gttcgccatc tactggcacc ttaccaactt gtttaaaacc
    15061 aagtttttca aaaatatgtt gcataacctt gttttcagca tgcgtatctg agcgaaaatc
    15121 aagataatca aaaccttcaa tcaagccctc taagaaggtt tgagcaactc cttttccctg
    15181 cacatctgct gccacagcaa tacggtgaaa gactagatac tctgactctc cagcttgcca
    15241 gtttccctca taaatagctt cataggcctc ctctggactc ttggtcacag cagcataggc
    15301 tagcagttct ccctcttcca aggctacgta ggcttgccct gagatgatat catcgataat
    15361 aacatctgca tttggatagc cattttgcca ctggtcacta ccagcatctg ctaaacattt
    15421 tttggcatcc tccatcacct gcatgatggc atctacttca tttggaaaag ctaaacgaat
    15481 ttccatcttt tttcctcatt tctgactagt ttctcccatc atagcataat ttaagtattt
    15541 ctacaagcag ttaaaacttg actttgtgtt tttgttattt tataatctag ttatcaaaac
    15601 tatataaaaa ggagttgaaa atgaaaacta ccttttccta cccaaaatgg gcagaaattc
    15661 caaacattga cctctatctg gaccaggttt tgctctatgt caatcaggtc tgcgccccta
    15721 tctctcctaa taaagacaag ggcctaacag catctatggt caacaattat gtgaaaaatg
    15781 gttacctgac aaagcctgac aaaaaaaaat accaacgcca acagattgcc cgtttgattg
    15841 ctatcacaac cctcaagtct gtattttcta ttcaagaaat agcccagaca cttaatactc
    15901 tacaaactca agcaagttca gaccaactct acgatgcttt tgtggactac atgaaccaag
    15961 gaattgaccc agctaaccct attatccaaa ccagctgcca aaccgtcaaa ctctatcatc
    16021 aaactctaga cttaatcgat catactcaag aggaggtaat ccaatgaaca ctagtcttaa
    16081 actcagcaaa caactcagtt ttggagagga gattgctaat agcgtgaccc atgctgtggg
    16141 tgcagtcatc atgcttatct tgctgcctat ttcatccatc tatagttatg aagcacacgg
    16201 atttttatcc tctatcggcg tttccatttt cgtcatcagt ctctttctca tgttcctatc
    16261 atccaccatt tatcactcta tggcctatgg ttcgacccac aaatatgttt tgcgaatcat
    16321 tgaccattct atgatttacg ttgccattgc cggctcatac acgcccgttg tcttgacctt
    16381 gatgaataac tggtttggct atctgattat tgtcatccaa tggggaacga ccatctttgg
    16441 tattctctat aaaatctttg ctaaaaaggt caatgagaaa tttagccttg ctctttacct
    16501 gattatgggc tggttggttc tggctatcat tcctgccatt atcagtcaaa cgacacccgt
    16561 tttctggagt ctcatggtaa ctggcggact ctgttataca gttggagctg gattttatgc
    16621 caagaaaaaa ccttatttcc acatgatttg gcatctcttt atcctagctg cgtccgcact
    16681 tcaatacatc gctattgttt attacatgta aaaaagttga gaaattcaat ctcaactttt
    16741 ttctttacac atattgataa agtactggtg caagcgcaca tcatcagtca attctggatg
    16801 aaaagaactt accaacatat ttttttcttg ggctgcaaca atttgattgt tcactgttgc
    16861 tagaatttct acaccctcac caacactact gataatcgga ccacggataa aggtcattgg
    16921 aatcttgcca actcccttac attctgcttc cgtgtagaaa cttcctaatt ggcgcccata
    16981 agcattacgc tcgaccacca tatccatagt tcctagatga ctctctttct gagaagtgat
    17041 ttccttagcc agcaaaatta agcccgcaca ggtcccaaac actggtaagc cagatagaat
    17101 ggcttctcgg atgggaagta gcatgttctg gtcacgtaag agcttgccca tggttgtaga
    17161 ctcaccacca ggcaaaatca aacccgacaa gtcactctga tcttgctgaa aatcatctag
    17221 atttctgagt tctacactct cgacacctaa ttgatctagc acttttgcat gttctgcaaa
    17281 ggccccttgc aaggccaata ttccaatttt catctatttt cctcgttcag ccatgagaat
    17341 ttggatttca ttttcattaa taccaaccat ggcttctcct aaatcttcag agatttgagc
    17401 taggatttga ggattacgga agttagtcac agccttaaca atggcactcg ctcgtttaac
    17461 aggatctcct gacttgaaaa tacctgaacc gacaaagacc ccctctgccc ctaattgcat
    17521 cattaacgca gcatctgctg gcgttgcaac acctccagca gcgaaattta caactggcaa
    17581 ttttccatgt tcatgaacat attggaccaa ttctacaggg acttgcaaat ccttggcagc
    17641 aacataaagc tcgtcctcac gtaagttttg aatgcggcga atttcctgat tcatcatacg
    17701 catatgacga acagcttgga cgatatcccc tgtccctggt tctcctttgg tacgaatcat
    17761 ggaagcacct tcagcgatac gacgcaaggc ttcacccaaa tccttagcac cacagacaaa
    17821 aggaacttgg aattctttct tgtccacatg gaaacggtca tcagctggag atagaacttc
    17881 actctcgtcg atataatcaa tttcaatagc ctctaaaatc tgagcttcaa caaaatgccc
    17941 gattctgacc ttagccatta ctggaatact aaccgcttct tggatttcct taatcatctt
    18001 tgggtcgctc atgcgggaaa ctcctccagc tgcacgaata tcagccggaa ttcgttccaa
    18061 ggccatcaca gctgccgcac cagcagcttc tgcgatacga gcctgttcag gattctgcac
    18121 atccataata acaccaccct tgagcatctg tgccaagttt ttatttagtt cataacgatt
    18181 ttcagtcatt tgttttatcc tcatcatttg tattggtagc taccttttca ttttaagtca
    18241 gaacagaatg aagcacaaga taaaaaaact ataattgtat ggggacagat tggctaaaaa
    18301 ctatctgacc ttaacttaag gccagatagc tcattcattt ttatttttca gccgtaaggg
    18361 cagccattgt gatgtagttg tatggtttat tgaagtgtgg caagaagaag aggtctgtca
    18421 atgccaattt atcaattgtc acatgctctt ggatagcaag tgagaacatg tggattccca
    18481 tgctaattgc aatatcatgt gaaaccattt gggcaccaag aatttcacgg ctatctttgt
    18541 caaagacaat cttaattgct acttcatggt tgtcatgttt catgaattct ggtttttgaa
    18601 gatcgttaaa gcctgtttca gttgcgttgt aaccagcagc tttcgctttt tcaagagtca
    18661 aaccagttga aaccatgtga agaccgtaga ttgagatacc atttgaacct tgaacaccga
    18721 ttccttccaa ttcatgtcca caagcgttgt aggcaccaac gataccagtg cgaacagcat
    18781 ttgaagcaag agcgatatag cttgtatctt tacgagcatt gtcataaaca gtcgcacagt
    18841 caccaacagc gtaaacgcct gggatagatg tttcttgttt cttgtctaca aggaaggcac
    18901 cgttgcggaa gagttcgatc ttaccatcag caagggctgt gtttggacgg aaaccaactg
    18961 caaggataac catatccacg tcaaagcttt ctttgtcagt aatcaagcgt tcaactttac
    19021 catcaccttc gattgcttta acagtttgac ctagagccaa gcggatgttg tgatcttcca
    19081 agttcttcgc catcatttgt gtgaagtctt tgtcatagta accgttcaag acagtatcaa
    19141 cgatatcaac aaggacaact tcttttccaa gacgttcaaa ggcttcagca agttcaacac
    19201 cgatgtaacc accaccaaca acggcgatac ggtcgagatg ttggctcttg tcagaaagtt
    19261 tattgataac ttcttcagca ttttggtaca atttcacgaa ttgtacgttt tcaagagttg
    19321 ctttaaattc gcggtttcct ttaacaattt caacaccttc gattggtggc aagattggtg
    19381 tagaacctgt agcgaaaatc aatttctcgt atgattcttt gtgttctttt ccttcaactt
    19441 ctgctgtaac cactttgtta tcatagtcga ttgaaagaac tggtgaattc atataaacct
    19501 tagcaccttt agcttctaat ttgtctttat cagaatagaa caagccttca gcaccgtcaa
    19561 tttgttcacc aatccaaaga gccattccac atcctaggaa agagatgtta gagttttggt
    19621 caaatacaac aatttcgttc tcatttccaa aattatccaa catggtattg atacatgctg
    19681 taccagcgtg gttagcaccg actacaacga ttttactcat aaaaaatttc ctgccttcat
    19741 tctaaattta ctttactagt ataccattta ccgttagcgt ttacaagggg gggactatta
    19801 tttcagcatt ttttatcaaa aataattccc catttatcct gttttcaagc ctcatgaatt
    19861 ttatgagcat atttcttgac tttttccaaa ttttgtttgt gaaaattcta acttcatggt
    19921 attataataa catgaaaata aaatgtaagg gcttcaattt agatgattat ttgatattgc
    19981 aacttatttt ctataagaga aaggagttgg tagcttgact cttcgttcac attctgaacg
    20041 gctaatgggg actactatca ctatttcatt agtggatgag caagccgata tctttctcca
    20101 aaaatccttt gacttgctca aagagcttga ataccgcttc aacgccaata gtcaagaatc
    20161 tgagttgatg gaaatcaatt atcaagcagg aatatccccc gtcacagttc atccagacct
    20221 gtttgagctg atttcacttg ggttagagca tagcctagcg ctctctagcc acctcaatat
    20281 cagcattggt cccttaattc aaacctggcg tatcggtttt tcagatgcca aggtcgccca
    20341 gcctcaagaa attgaatcgg tgctgcctct aatcaatcct catggtatcg agttagattc
    20401 ttctacttct actgtgtttt taaaacagaa aggaatgaag atcgatctag gttgtttagc
    20461 caaaggatac agtgcggata aggttgccca atttcttagg aaagaggggg tgacttctgc
    20521 cttgatcaat ctgggaggga atatcctgac cattggaaaa aatcaggcaa gaggggataa
    20581 cccatggcaa atcgggattc aagacccagc caatcctagg ggaaatcatt taatgaccat
    20641 ccctgttgtc aataaatctg tcgtgacttc aggcatttat gaacgtcacc tgaccgtcga
    20701 tggacaagat taccatcaca tttttgacag tcaaacagga tatcctgttg aaacggaact
    20761 agcgagtcta acaatcatct ctgataaatc agtcgatggc gaaatctgga cgactcgact
    20821 ctttggagaa agacctgctt ctatcctctg gcaagtcgaa agtttggagg gcatcgaagt
    20881 catcctcatc gataaagaag gccacctaag ctgttcttca ggaattccta ctctatagag
    20941 acaactgttt caatggaatt ttaaaattgt attatgtaaa aagagaaagg aaatatcatg
    21001 ttaaaactta ttgctattgt tggaacaaat tcaaaacgtt ctacaaaccg tcaattgctt
    21061 caatacatgc aaaaacactt tgctgacaaa gctgaaattg aacttgttga aatcaagacc
    21121 attcctgtct tcaacaaacc agctgacaag caagtacctg ctgaaatatt ggaaattgct
    21181 gctaaaatcg aagaggcaga tggcgttatt atcggtactc ctgagtatga tcactctatt
    21241 ccagctgttt tgatgagcgc tcttgcttgg ttgtcttatg gtatttaccc acttttgaac
    21301 aaaccaatca tgattacagg tgcttcttac ggtacgcttg gttcatctcg tgcccaattg
    21361 caacttcgtc aaatcttgaa tgctcctgaa atcaaggcaa atgttcttcc agatgaattc
    21421 ttgctctcac actctcttca agcatttaac ccaagtggcg acttggttga ccttgatgtt
    21481 atcaagaaat tggatgccat ctttgatgac ttccgtatct tcgtgaagat tactgagaaa
    21541 ttgcgcaatg cgcaagaatt gcttcgcaaa gatgctgaag actttgactg ggaaaatttg
    21601 taagatagga gaccgaaaga atgaaatttg ttggacttgt tggatcaaac tacgatcaat
    21661 catataaccg caaactcttg gaatttatcc gtcgcaattt caaattcaaa tttgaattag
    21721 aagtacttga aatcgacgaa gttccaatgt ttaaccaaga cgaaaaatgg gacgaaagct
    21781 tccaattgcg tttcttgtat aacaagatta cacgtgctga tggtgtcatt atcgccactc
    21841 ctgagcacaa ccacactatc tcagcttcac tcaaatctgt acttgaatgg ctttcatacg
    21901 aagttcatcc atttgaaaac aagcctgtta tgattgtggg tgcatcatac tatgaccaag
    21961 gaacatcacg tgctcaagtt caccttcgta aaatccttga tgctccaggt gttaatgcct
    22021 acacgcttcc aggtaacgaa ttccttcttg gtaaagctaa ggaagctttt gataacaacg
    22081 gaaatatcac caacgaaggc actgttaaat tccttgaaac ttgcttagat aactttgtta
    22141 aatacgtagg agtcgtttcg aaattgaaaa aaccaaaacc aatcgaatca gaagacttgg
    22201 attgtggaaa accaatcgct acaaccatca cagaagttga ccctgacgat ccagaatggg
    22261 tagaaaaagt tgcagcaatc actggtgctg tttctggtga tacctatgtc aaattggacc
    22321 acggtatcct aacagttaac caaattgata tgttcttgaa agctatgcca tttgaattga
    22381 catacgctga cgacaacaac caattcctct actacaacaa cgctcaccaa gatccagaca
    22441 ctatgttcgc taaacgtgta ccacctcaat caggtagccg tatgtcgact gttcatggtt
    22501 ctcttccacc agcacgtatg aagaacgtag agtgggttat cggaacactt cgcaacggaa
    22561 accaagaata cgtccgtacg atcgttccag gttctcctgc aggtgttatc aacactcaca
    22621 actaccaagc aatgtactat cctgatggat catacgctgg tatcaatgaa atcgtcttta
    22681 acttccaacc atggcttgac tggtacctaa aagagactgg tcaacgtttg gtgggtggta
    22741 gcggaccatt tgctcctgct gccggaggtc atggagacgc cgatgctact tctggcgctt
    22801 ctgattcagg ggatgctgga ggccacggtg gtgacgcaga cgctacagcc ggtgcaagtt
    22861 a
//
LOCUS       DAVBCN010000027        22343 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVBCN010000027 DAVBCN010000000
VERSION     DAVBCN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22343
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.027"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(171..395)
                     /locus_tag="VQ497_000993"
     CDS             complement(171..395)
                     /locus_tag="VQ497_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory protein Asp5"
                     /protein_id="HET2517318.1"
                     /translation="MIEMLIVLAIILSLALIVLVTIQPRQNQLFSMDATSNIGKPSYW
                     QSNTLVKVLTLLVSLALFVLLLTFMVITYK"
     gene            complement(400..579)
                     /gene="asp4"
                     /locus_tag="VQ497_000994"
     CDS             complement(400..579)
                     /gene="asp4"
                     /locus_tag="VQ497_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory system protein Asp4"
                     /protein_id="HET2517319.1"
                     /translation="MSEEDLFYKDVEGRMEELKQKPIKKEKETRGEKISKTFSLLLGL
                     MILIGLLFTLLGILR"
     gene            complement(581..1918)
                     /gene="gtfB"
                     /locus_tag="VQ497_000995"
     CDS             complement(581..1918)
                     /gene="gtfB"
                     /locus_tag="VQ497_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HET2517320.1"
                     /translation="MIELYDSYSQESRDLHESLVATGLSQLGVVIDADGFLPDGLLSP
                     FTYYLGYEDGKPLYFNQVPVSDFWEILGDNQSACIEDVTQERAVIHYVDGMQARLVKQ
                     VDWKDLEGRVRQVDHYNRFGACFAKTTYSADSEPIMTVYQDVNGQQVLLENHVTGDIL
                     LTLPGQSMRYFANKVEFITFFLQDLEIDTSQLIFNTLATPFLVSFHHPDKSGSDVLVW
                     QEPLYDAIPGNMQLILESDNVRTKKIIIPNKATYERALELTDEKYHDQFVHLGYHYQF
                     KRDNFLRRDALILTNSDQIEQVEAIVEALPDVTFRIAAVTEMSSKLLDMLRYPNVVLY
                     QNASPQKIQELYQLSDIYLDINHSNELLQAVRQAFEHNLLILGFNQTVHNRLYIAPEH
                     LFESSEVAALVETIKLALSDVDQMRQALGKQGQHANYVDLVRYQEIMQTVLGG"
     gene            complement(1911..3422)
                     /gene="gtfA"
                     /locus_tag="VQ497_000996"
     CDS             complement(1911..3422)
                     /gene="gtfA"
                     /locus_tag="VQ497_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HET2517321.1"
                     /translation="MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMIL
                     ADNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVL
                     RVFFFDQDKFVTCYLVDENKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAV
                     LYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVIL
                     DRETGIGQVVFEEAQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNADKVDFFI
                     VSTDRQNEVLQEQFAKYTQHQPKIVTIPVGSIDSLTDSSQGRKPFSLITASRLAKEKH
                     IDWLVKAVIEAHKELPELTFDIYGSGGEDSLLREIIANHQAEDYIQLKGHAELSQIYS
                     QYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVE
                     DQIKQAYAAKICQLYQENRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD"
     gene            complement(3434..5806)
                     /gene="secA2"
                     /locus_tag="VQ497_000997"
     CDS             complement(3434..5806)
                     /gene="secA2"
                     /locus_tag="VQ497_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HET2517322.1"
                     /translation="MFRRLGQDFQLRKVKKILKQINALKGKMSSLSDQELVAKTVEFR
                     QRLSEGESLDDILVEAFAVVREADKRILGMFPYDVQVMGAIVMHYGNVAEMNTGEGKT
                     LTATMPVYLNAFSGEGVMVVTPNEYLSKRDAEEMGQVYRFLGLTIGVPFTEDPKKEMK
                     AEEKKLIYASDIIYTTNSSLGFDYLNDNLASNEEGKFLRPFNYVIIDEIDDILLDSAQ
                     TPLIIAGSPRVQSNYYAIIDTLVTTLVEGEDYIFKEEKEEVWLTTKGAKSAENFLGID
                     NLYKEEHASFARHLVYAIRAHKLFTKDKDYIIRGNEMVLVDKGTGRLMEMTKLQGGLH
                     QAIEAKEHVKLSPETRAMASITYQSLFKMFNKISGMTGTGKVAEKEFIETYNMSVVRI
                     PTNRPRQRIDYPDNLYITLPEKVYASLEYIKQYHAKGNPLLVFVGSVEMSQLYSSLLF
                     REGIAHNVLNANNAAREAQIISESGQMGAVTVATSMAGRGTDIKLGKGVAELGGLIVI
                     GTERMESQRIDLQIRGRSGRQGDPGMSKFFVSLEDDVIKKFGPSWVHKKYKDYQVQDM
                     TQPEVLKGRKYRKLVEKAQHASDSAGRSARRQTLEYAESMNIQRDIVYKERNRLIDGS
                     RDLEDVVVDIIERYTEEVAADHYASRELLFHFIVTNISFHVKEVPDYIDVTDKTAVRS
                     FMKQVIDKELSEKKELLNQHDLYEQFLRLSLLKAIDDNWVEQVDYLQQLSMAIGGQSA
                     SQKNPIVEYYQEAYAGFEAMKEQIRADMVRNLLMGLVEVTPKGEIVTHFP"
     gene            complement(5799..6239)
                     /gene="asp3"
                     /locus_tag="VQ497_000998"
     CDS             complement(5799..6239)
                     /gene="asp3"
                     /locus_tag="VQ497_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HET2517323.1"
                     /translation="MIITQRQSIYWGEVGGTYMYGTTVSYYLDKSVRLYNPLLPSGEI
                     LKTWFSSVNYQAARTQPQLPLLKRKQEYQLSLVFDCQPENGVYTKITFFDRYGDILEK
                     KVEKAKDFIFTYPEDSYTYQVSLLSAGFESLTFYHFSIKEIRSV"
     gene            complement(6236..7771)
                     /gene="asp2"
                     /locus_tag="VQ497_000999"
     CDS             complement(6236..7771)
                     /gene="asp2"
                     /locus_tag="VQ497_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HET2517324.1"
                     /translation="MSKELNILQIGLANWENHYDIPENMSWYYFYPNSSKTLREIIEK
                     EDINRFHAVLIEDGQYSRDLFSYVKYFEPYTLFYNQNLQINDREVVDFLKKRCAQAID
                     FLSPQQLINDLSKSLFGGGYGDKLFPPTIQVNPNFTGAISYQGLDYVSLEGEFGQDFA
                     QLAYWAYNIMVQKTLPIELWLEYEKEGNCDFRLVIRKMWSGSVDDFFEEVIVSEKDLE
                     QALFMDSRDGDYFLSISVEARGRGTIKLGNLHQRWSRKQFGKFVLGGNILHDSKRDEI
                     NYFFHPGDFKPPLTVYFAGYRPAEGFEGYFMMKTLGCPFILFSDPRLEGGAFYLGTDE
                     LEGKVKDTITHYLDYLGFDHKDLILSGLSMGTFPALYYGASFEPHAIIVGKPLANLGT
                     IASRGRLDAPGVSNLAFDCLIHHTGGTSSQDMTELDQRFWKIFKQANFSKTTFGLSYM
                     KDEEMDPQAYEQLVSYLCNTGAKILSKGTAGRHNDDTDTNISWFLHFYRMVLETGFGR
                     EKR"
     gene            complement(7755..9335)
                     /gene="asp1"
                     /locus_tag="VQ497_001000"
     CDS             complement(7755..9335)
                     /gene="asp1"
                     /locus_tag="VQ497_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HET2517325.1"
                     /translation="MYYFIPAWYGSERTWHADITPWYFSHFRLEFDDTFHQIRLFQEQ
                     DIDSRLLVLAYQPHLRYFLYRHGVLETDTYSVFDVMQDFHNLHTQVLSIRDIEWDDDC
                     EFIYSPFTIIVQKNGKKFAKVEHGVEGFISDIQYFEPNGQIHMHHIMDDRGFISSIIF
                     FEDGQAAYQEYLNPKGVWQFRERLKEGGQVEVNPILGYRFKMLTYQNMGDLVAEFFEN
                     YLQTYVKDQDIFMLPSHSHHDQLVLDRLPSTNPKLLSLFIGRNPQDTFRDLDVTFEKS
                     DLILVDREDSLRLLQELYPERMHQCYHLSSFDTRLRLGRSQTKKESIIYFQLDFEQGI
                     DNQALLQVLSFVAENKDTEVIFGAFAASQEQMNEVEGIVESFIQENIQSENLGKAIDY
                     GDAENPLEENQHQDLRLQFVNLNDELDLIKTLEFVRLIVDLNRHPHLYTQIAGISAGI
                     PQINLVETVYVEHLKNGYLLTDVTEFSKAAHYYTDRLKEWNESLIYSIDKIKEHTGQQ
                     FLGKLEKWIEEVKNVKGT"
     gene            complement(9345..10562)
                     /gene="secY2"
                     /locus_tag="VQ497_001001"
     CDS             complement(9345..10562)
                     /gene="secY2"
                     /locus_tag="VQ497_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HET2517326.1"
                     /translation="MTKIYSSIAVKKGLFTLFLLFIYVLGSRITLPFVDLNTKDFLGG
                     STAYLAFSAALTGGNLRSLSIFSVGLSPWMSAMILWQMFSFSKRLGLTSTSIEIQDRR
                     KMYLTLMIAVIQSLAVSLRLPVQSSYSAILVVLMNTILLIAGTFFLVWLSDLNASMGI
                     GGSIVILLSSMVLNIPQDVLETFQTVHIPTGIIVLLALLTLVFSYLLALMYRARYLVP
                     VNKIGLHNRFKRYFYLEIMLNPAGGMPYMYVMSFLSVPAYLFILLGFIFPNHSGLAAL
                     SKEFMIGKPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGADTSR
                     FINRLVLRFSVIGGLFNVVMAGGPMLFVLFDEKLLRLAMIPGLFMMFGGMIFTIRDEV
                     KALRLNETYKPLI"
     gene            complement(10619..11296)
                     /locus_tag="VQ497_001002"
     CDS             complement(10619..11296)
                     /locus_tag="VQ497_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET2517327.1"
                     /translation="MGISVAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNY
                     YNYDESDGNFYFYVTGQDFCVEELAIQEIMNRQAGDWKFNSSAFILPTFKLIKKELFN
                     EVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVE
                     VFSKKISDCVLAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDA
                     EQRNGKS"
     gene            complement(11281..11526)
                     /locus_tag="VQ497_001003"
     CDS             complement(11281..11526)
                     /locus_tag="VQ497_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2517328.1"
                     /translation="MSELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDGSTD
                     NSGEICDAFMMQDNRVRVLHQENKGGGQHKLKIWGLV"
     gene            complement(11526..12722)
                     /locus_tag="VQ497_001004"
     CDS             complement(11526..12722)
                     /locus_tag="VQ497_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HET2517329.1"
                     /translation="MRKSIVLAADNAYLIPLETTIKSVLYHNRDVDFYILNSDIAPEW
                     FKLLGRKMEVVNSTIRSVHIDKELFESYKTGPHINYASYFRFFATEVVESDRVLYLDS
                     DIIVTGELATLFEIDLKGYSIGAVDDVYAYEGRKSGFNAGMLLMDVAKWKEHSIVNSL
                     LELAAEQNQVVHLGDQSILNIYFEDNWLALDKTYNYMVGVDIYHLAQECERLDDNPPT
                     IVHYASHDKPWNTYSISRLRELWWVYRDLDWSEIAFQRSDLNYFERSNQSKKQVMLVT
                     WSADIKHLEYLVQRLPDWHFHLAAPCDCSEELTSLSQYTNVTVYQNVLHSRIDWLLDD
                     SIVYLDINTGGEVFNVVTRAQESGKKIFAFDITRKSMDDGLYDGIFSVERPDDLVDRM
                     KNIEIE"
     gene            complement(12731..13951)
                     /locus_tag="VQ497_001005"
     CDS             complement(12731..13951)
                     /locus_tag="VQ497_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HET2517330.1"
                     /translation="MRNTKRAVAFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIP
                     QEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKV
                     LYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
                     KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIP
                     LEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIF
                     KLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPM
                     LLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSS
                     IPKKMVAAIRSYMR"
     gene            complement(13982..16426)
                     /locus_tag="VQ497_001006"
     CDS             complement(13982..16426)
                     /locus_tag="VQ497_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HET2517331.1"
                     /translation="MIKTIVLAGDRNYIRQLETTIKSILYHNRDVKIYILNQDIMPDW
                     FRKPRKIARMLGSEIIDVKLPEQTVFQDWEKQDHISSITYARYFIADYIQEDKVLYLD
                     SDLIVNTSLEKLFSICLEEKSLAAVKDTDGITFNAGVLLINNKKWRQEKLKERLIEQS
                     IVTMKEVEEGRFEHFNGDQTIFNQVLQDDWLELDKEFNLQVGHDVTAFYNKWENYFNE
                     LVPPSIIHFVSYRKPWTTLIANRYRDLWWEFHDLEWTKILQHHIGEFELTSSLDKEFS
                     CLTLTNSQDLEGIEELVTALPDVVFHIAAWTDMGDKLIKLAVYDNVRLHPQIVPPVLD
                     KLKKSTNLYLDINHGSADENFLKSLQEQEKTLLAFQSTQHGELGQIVFENGKVSFMID
                     TIKDFKKNGHLTCFRQLPSLTCLTFTASQDIEQLDYLAGQLPNVVFQVAAWTAMGPKL
                     YDLSNRYPNIQLYPAISRDKLDELKEKMDAYLDINLLTSTSDIVAEMAHLSKPILAFY
                     KSQNGNNGQRLYSSEHPERMLADLQKLITKDMLEKPLDIIQVKGIDETLDYIIEHNSS
                     LVRFGDGEINMLAGHSIPYQDYDEELVSIMRDIIGQESREDLVVCLPDAFTDRFRFTS
                     WAIPFWKDHMDHYMDFYRELCSDSWYGSTFVSRPYIDFEDKSQAKAQFEKLKSIWENR
                     DLLIVEGATSRSGVGNDLFDEANSIKRIICPSHSAFSRVHEIEQEIEKYAGGRLILCM
                     LGPTAKVLSYNLCQMGYQVLDVGHIDSEYEWMKMGAKTKVKFSHKHTAEYNFDQDIEF
                     IDDETYNSQIVARILN"
     gene            complement(16436..17446)
                     /locus_tag="VQ497_001007"
     CDS             complement(16436..17446)
                     /locus_tag="VQ497_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET2517332.1"
                     /translation="MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIA
                     SDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDV
                     VPLMFDSNYYLMKDYMYMYNLSDVLIVPSERMKTRLMEEGLTTKKILVQGMWDHPHDL
                     SLYTPAFKKELFFAGSLERFPDLQNWSQDTPLRVFSNKGEASSSARNLSIEGWKKDEE
                     LLLELSKGGFGLVWGTYQNDGESNQYYTLNISHKVSTYLTAGIPVIVPSSLSTAKFIV
                     DQGLGFVANSLEEVHAIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLLVDAIYHLG
                     VE"
     gene            complement(17475..18350)
                     /locus_tag="VQ497_001008"
     CDS             complement(17475..18350)
                     /locus_tag="VQ497_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2517333.1"
                     /translation="MENELISIVVPIYNVENYLRQCLDSIQKQTYPNFECIMVNDGSP
                     DHSSKICEEFVEKDSRFKYFEKANGGLSSARNLGIECSGGAYITFVDSDDWLEHDALD
                     RLYGALKKENADISIGRYNSYDETRYVYMTYVTDPDDSLEVIEGKAIMDREGVEEVRN
                     GNWTVAVLKLFKRELLQDLPFPIGKIAEDTYWTWKVLLRASRIVYLNRCVYWYRVGLS
                     DTLSNTWSEKRMYDEIGAREEKIAILASSDYDLTNHILIYKNRLQRVIAKLEEQNMQF
                     TEIYRRMMEKLSLLP"
     gene            complement(18390..19268)
                     /locus_tag="VQ497_001009"
     CDS             complement(18390..19268)
                     /locus_tag="VQ497_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HET2517334.1"
                     /translation="MKKNHLVGDALILTVSDQIEELDYLLESLPNICFYIAAPVQFSE
                     KIRSLETNYNVRLRTITNEEQLNFLVDTCDFLLDINHFQEVDAIVSKFVQAGKSVFAF
                     DNTVHGNQGQEVFLSSTPDKLVSRVRDYLNEVRVGTNHQEKIIQDGTWNVFKIDDKAH
                     FIVGANVICRNFENFHVSSGKLILHDGVFINNSCSFNCMERIEIGAGTMMGEGVRFYD
                     HDHIYTAEKIEKWQWTTAPIRVGRDCWIGSNVTILKGVTIGDNTIIGAGCLIRNDIPS
                     NSVVYNNGNLVVKRRD"
     gene            complement(19265..21350)
                     /locus_tag="VQ497_001010"
                     /pseudo
     CDS             complement(19265..21350)
                     /locus_tag="VQ497_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000341103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(21411..>22343)
                     /locus_tag="VQ497_001011"
     CDS             complement(21411..>22343)
                     /locus_tag="VQ497_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2517335.1"
                     /translation="ASTSASASASTSASASASTSASESASTSASASASISASESASTS
                     ASASASTSASASASTSASESASTSASASASTSTSASESASTSASASASTSASASASTS
                     ASESASTSTSASESASTSASASASTSASASASTSASASASTSASASASTSASASASTS
                     ASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSAS
                     ASASTSASESASTSASESASTSASASASTSVSNSANHSNSQVGNASGSTGKSQKELPN
                     TGTESSIGSVLLGVLAAVTGIGLVAKRRKRDEEE"
BASE COUNT         7082 a         4766 c         3552 g         6943 t
ORIGIN      
        1 acaacttcaa aacggtgttt tgagctgact tcgtcagttc catccacaac cttaaaacag
       61 tgttttgagc agcctgcggc tagcttccta gtttgctctt tgattttcat tgagtataaa
      121 aaagaaaact tcagaccaat ccacaaaagg tgaatatctg aagttttctt ttatttataa
      181 gtaatcacca taaaggttaa tagtagaaca aataaagcca aactcaccaa taaagtgagc
      241 accttgacca aggtgttgct ctgccagtag cttggtttac caatattact agtggcatcc
      301 atggaaaata gttgattttg acggggttgt atagttacca atacaatcaa agcaagagat
      361 aggataatag ctaaaacaat tagcatttca atcattgatc tacctcaaaa ttcccaacaa
      421 agtaaagagc aaaccaatca gaatcattaa gcccagtaaa agtgaaaaag ttttactaat
      481 cttctcccct cgggtttctt tttccttctt gatgggtttt tgtttcaact cttccatgcg
      541 accttcaacg tctttgtaaa ataaatcttc ctctgacatg ttagcctcct aaaacagttt
      601 gcattatttc ttgatatcgc accaagtcaa cataatttgc atgttggcct tgtttgccaa
      661 gtgcctgacg catttgatca acatctgaaa gggccaattt aatggtctca accaaggcag
      721 caacttcact actttcaaat agatgttctg gagcgatata aagtctattg tgaaccgttt
      781 ggttaaatcc aagaatcaag agattgtgct caaaggcctg acgcactgcc tgcaacaact
      841 cattgctgtg attgatatcc aagtaaatat ccgacagttg atacagctcc tgaatcttct
      901 gtggactagc attctggtaa aggaccacat taggatagcg aagcatgtct aagagcttag
      961 aagacatctc tgtcactgct gcgatacgga aggtaacatc aggcaaggct tctacgattg
     1021 cttctacttg ctcaatctga tctgaattgg tcaagattaa ggcatctcgt cttaggaaat
     1081 tatcacgttt gaactggtaa tgataaccca agtgcacaaa ctgatcatgg tatttctcgt
     1141 cagttaactc taaagcgcgc tcataagtcg ccttatttgg aatgatgatc ttcttagtac
     1201 gcacattatc actttccaaa atcaactgca tattacctgg aatggcatca tagagaggtt
     1261 cctgccatac caagacatcc gagccagatt tatctggatg atggaaggaa accaagaaag
     1321 gagtcgctag agtattaaag ataagctgac tggtatctat ttccaaatct tgcaaaaaga
     1381 aggtgataaa ttcaacttta tttgcaaagt aacgcatgga ctgaccaggc aaagtcaata
     1441 agatatcacc cgtcacatgg ttttccagta aaacttgttg gccattgaca tcttggtaaa
     1501 ctgtcataat cggctcgcta tctgcgctat aagtggtttt agcaaagcaa gcaccaaagc
     1561 gattgtagtg gtcaacctga cgtactcgac cttctaggtc tttccagtct acctgtttaa
     1621 ccaagcgagc ctgcattcca tcaacataat gaatgacagc cctctcctgc gtcacatctt
     1681 caatacaagc agactgatta tctcctaaaa tttcccaaaa atctgaaacg ggaacttggt
     1741 taaaatagag aggttttcca tcctcgtaac ctagataata ggtaaaagga gaaagcagac
     1801 catcaggcag aaaaccatct gcatcgatga ccactccaag ttgagaaaga ccagtagcga
     1861 ctagactttc atgtaaatct ctactttctt gactgtaact atcataaagt tcaatcatgg
     1921 agcacctcct ctactgtttt cttccacttt tctaaaattt ctttggtcaa gaagccttct
     1981 gcaatttgat aagaataggc acgcatagct tccaaacgat tttcttgata caattgacaa
     2041 atcttagcgg cataagcttg cttgatttgg tcttctacat ggtcagatga acttggaatc
     2101 aaataaccat tttgcccatc ctctataaag gtctgattac cataaggcac atcaaaacca
     2161 attagaggta gacctgaacc aatagcttcc atcaaggtca gaccaaatcc ttcgctggta
     2221 gaagccgtta agtagacctc atactggcta taaatctgcg aaagttccgc atgccccttg
     2281 agttggatat agtcctctgc ctgatgattt gcaataattt ctctaagcag agaatcttct
     2341 ccaccactac catagatatc aaaggttagt tccggtaact ccttatgagc ttcaatcaca
     2401 gctttcacaa gccaatcaat gtgcttttct ttggcaagac gtgaagccgt aatcaatgaa
     2461 aatggtttgc gcccttgact tgaatctgtc aaggaatcaa tactgcctac aggaatggta
     2521 acaatctttg gctgatgctg agtatatttg gcaaattgct cttgtagaac ttcattttgt
     2581 ctatcagtag acacgataaa gaagtcaacc ttatctgcat tggtaaactg atagtcataa
     2641 tagttattcc aaaggatata gtcctcattt gtagcatttt cactataatg ctccgcatga
     2701 acaactaccg ctagatgtgc tgtctgtgct tcctcaaaca caacctgtcc aatacctgtc
     2761 tccctatcca gaataaccaa atcagacttg ttcaaattca aagatttcat aaaggcacgc
     2821 acaaaagctt gctttccata gaaaatctta tccttgaaat gataaacttc ttccttccct
     2881 tgattcatca aaatatcata gactggagtc ccgtcttcat tgtaaaaagt tcgttggtat
     2941 aagactgcaa cattgtcctt gggagcaaaa tactcgctac aataacgcgt ataagaaaag
     3001 taatccttcc gaatcaggtt tcccttaaaa acatactcgg catgttgaac caagtccttg
     3061 ttctcatcaa ccaaataaca ggttacaaac ttatcttggt caaaaaagaa tacacgtaaa
     3121 accttgccat ttttttctct gtgactttct tcaccaccaa agtaagccaa gacatcatcc
     3181 actgtcacgc tagtaggtgc aattttgata tctgtgaaat gattataaag ccagataacc
     3241 tgattatcat caaaaccaat attggctgtt aagtgctgaa tattatcggc taaaatcata
     3301 tctgtaaaga taaacttaga ggacagattt aatttccgaa aaacaccagc acgataggct
     3361 tgagcgtatt caacaccgct actagcccaa ccaattccta aatttatatt gtaaattgtc
     3421 atattttctc ctcttatgga aaatgagtca cgatttcacc ttttggagtg acctcaacca
     3481 gccccatcag gagattacgc accatatccg cacggatttg ttctttcata gcttcaaagc
     3541 ccgcgtaggc ttcttgatag tactctacga ttggattttt ctgactagca gattgaccac
     3601 cgatagccat ggatagctgt tgtagatagt ctacctgctc tacccagttg tcatcaatgg
     3661 ctttaagcag tgaaagtcgt aaaaactgtt catataagtc atgttgatta agtaattctt
     3721 tcttttcaga aagttcttta tcaatcacct gcttcataaa gctacgaact gcagttttgt
     3781 cagttacatc tatataatct ggaacctctt taacatgaaa actaatattg gtcacaataa
     3841 agtgaaacaa taattcacga ctagcatagt gatcagccgc tacctcttct gtatatctct
     3901 caatgatatc cacaacaaca tcctctaagt cacgagaacc atctattaga cgatttctct
     3961 ctttatagac tatatcccgt tgtatattca tactttcagc atactccaga gtctgacgac
     4021 gtgctgaacg tccagcacta tcactggcat gctgagcctt ttcgactagt ttccggtatt
     4081 tacgaccttt caatacttcc ggttgagtca tatcttgaac ctgatagtct ttgtactttt
     4141 tatgcaccca agatggacca aatttcttga taacatcatc ctctaaggat acaaaaaatt
     4201 tactcatacc aggatctccc tgacgaccag aacggccacg aatttgtagg tcgatccgct
     4261 gactttccat ccgctcagtc ccaataacaa tcaagccccc aagctctgcg actcctttac
     4321 caagcttgat atccgtacca cgtcctgcca tagaggtagc cactgtcaca gcccccatct
     4381 gacctgactc ggagataatc tgagcctcac gcgccgcatt attagcattt aggacattat
     4441 gggcaatccc ttcacgaaac aagagagacg aatagagttg agacatttca actgagccta
     4501 caaaaacgag taaaggattt cccttagcat ggtattgctt gatgtactcc aaggatgcat
     4561 acactttttc aggtaaagtg atatatagat tatctggata gtcaatccgt tgtctcggac
     4621 gattggttgg aatgcgtact acagacatat tgtaagtttc aataaactct ttttccgcga
     4681 ccttacctgt ccctgtcata ccagatatct tattaaacat cttaaaaaga ctctgatagg
     4741 tgatcgaggc catagcccgc gtctcaggag ataatttgac atgttccttg gcttcaatag
     4801 cctgatggag acctccttga agtttagtca tttccattag acgccctgtt cccttatcaa
     4861 ccagtaccat ctcatttcca cgaatgatat agtccttatc tttagtaaag agcttatgag
     4921 ctcgaatcgc ataaaccaaa tgacgagcaa aagacgcatg ctcttccttg tataaattat
     4981 caatccctag gaaattctca gcagacttgg cccccttagt agtgagccaa acctcctctt
     5041 tctcctcttt aaagatataa tcctctcctt cgaccaaggt tgttacaagt gtatcaatga
     5101 tcgcatagta attagactga acacgaggag aacccgcaat aatcagagga gtttgtgcac
     5161 tatcaagcaa gatatcatca atttcatcaa taataacata gttaaacggt cgtaaaaact
     5221 taccttcttc attcgaggct aggttatcat ttagataatc aaaacctaaa ctactattgg
     5281 ttgtgtagat gatatccgaa gcatagataa gcttcttttc ttcagctttc atttccttct
     5341 ttggatcttc cgtaaatggt acaccaatgg tcaatcctag aaaacgataa acttggccca
     5401 tttcctcggc atcacgcttt gataaatact cattaggagt cacaaccatc actccttctc
     5461 ctgaaaaagc gttcaaatag acaggcatgg tagctgtcaa ggtcttacct tcccccgtat
     5521 tcatctcagc aacatttcca tagtgcatga caatagctcc catgacttga acatcatagg
     5581 ggaacatccc taaaatccgc ttatctgctt cacgcaccac agcaaaagct tcaaccaaaa
     5641 tatcgtctag actttctccc tcggaaagac gctgacgaaa ctctactgtt ttagctacta
     5701 attcttgatc cgaaagagag gacatcttgc ctttcagggc attaatctgc tttaaaatct
     5761 ttttcacttt cctaagctgg aaatcttgac ctaaacgtct aaacacttct gatctccttg
     5821 atagaaaaat gataaaaagt taaggactca aatccagcac ttaaaagaga aacttgataa
     5881 gtataactat cttctggata agtaaatatg aaatctttcg ctttttctac ctttttttct
     5941 aaaatatcac catagcggtc aaaaaaagtt atcttggtat aaactccatt ttcaggctga
     6001 caatcaaaaa ccagtgatag ttgatactcc tgctttcttt ttaataaggg aagctgaggt
     6061 tgggttcgtg cagcctggta attgacacta gaaaaccaag tctttagaat ctcaccagat
     6121 ggcaatagag gattatacag acgaacactt ttgtcaaggt aataagatac agttgttcca
     6181 tacatataag tcccaccaac ttctccccaa taaatagact gtctctgtgt aataatcatc
     6241 ttttctccct tccaaaacca gtctctaaga ccattctata aaagtgcaaa aaccaagaaa
     6301 tattggtatc tgtatcatca ttgtgtcgtc cagcagttcc tttagataaa atcttcgcac
     6361 ctgtattaca cagataagac actaattgtt cataggcttg tggatccatt tcttcatctt
     6421 tcatatagga taaaccaaag gttgtctttg aaaaatttgc ttgtttaaaa attttccaaa
     6481 aacgctgatc caactccgtc atatcttgag aacttgtccc acctgtatga tgaattaaac
     6541 aatcaaaagc taagttagag acacccggtg cgtccaaacg tccacgacta gctatagttc
     6601 ctaaattagc caagggctta ccaactatga tggcatgggg ttcaaaagaa gcaccataat
     6661 agagagccgg aaacgttccc atagaaagac ctgataaaat caaatcctta tgatcaaagc
     6721 ctaaataatc aagatagtga gtgatcgtat cctttacttt tccctctagc tcatccgttc
     6781 cgagataaaa agctcccccc tctaaacgtg gatcagaaaa taaaatgaag ggacatccaa
     6841 gagttttcat cataaagtaa ccctcgaatc cttctgcagg tcgataacct gcaaaataga
     6901 cagtcaaagg cggtttaaaa tcacctggat ggaagaaata gtttatttca tcacgcttgg
     6961 aatcatgtag gatatttcca ccaagtacaa acttaccaaa ttgttttcga ctccatcgtt
     7021 ggtgaagatt acctagtttg atagttccac gacctcttgc ttcaacagat atcgagagaa
     7081 agtagtctcc gtctcgacta tccataaaaa gtgcttgctc caagtctttt tcagatacta
     7141 ttacttcttc aaagaaatca tcaacagacc cactccacat tttacggatt actaaacgaa
     7201 agtcacaatt gccttccttc tcatattcaa gccacaactc aatagggagt gttttttgca
     7261 ccataatatt ataagcccaa taggcaagct gggcaaaatc ttgcccaaac tcaccttcca
     7321 aactgacata atccaatcct tgataagaaa tagctcctgt aaaatttgga ttgacttgta
     7381 tcgtcggagg aaagagttta tcaccatacc caccgccaaa aagagattta cttaaatcat
     7441 tgattagttg ttggggactt aaaaaatcaa ttgcttgtgc acatcgtttt tttagaaaat
     7501 ccacaacctc tctatcattt atttgtagat tctggttata gaacaaagta taaggttcaa
     7561 aatattttac ataggaaaat aagtctctgg aatactgacc atcttctatt aaaactgcat
     7621 gaaaacggtt aatatcctct ttttcgatta tttcacgaag ggtttttgat gagtttgggt
     7681 aaaaataata ccaactcata ttttcaggta tgtcatagtg attttcccaa ttggcaagtc
     7741 ctatctgtaa aatattaagt tcctttgaca tttttaacct cctctatcca tttctctaat
     7801 tttccaagaa attgttgtcc tgtgtgctcc ttaatcttat caattgaata tatcaaggac
     7861 tcattccact ccttcaacct atctgtgtaa taatgtgcag ccttagaaaa ttctgtcaca
     7921 tctgtcagca aataaccatt ttttaaatgt tccacataga cggtttcaac taggttgatt
     7981 tgaggaattc ctgcactaat cccagcaatc tgtgtgtaga gatgaggatg tctatttaaa
     8041 tccacaatca aacggacaaa ttctagtgtt tttatcaaat ctaactcatc attcaagtta
     8101 acaaactgta agcgtaagtc ctggtgttga ttttcttcca ggggattttc tgcatcacca
     8161 taatcaatcg cctttcccag attttcggat tgaatgtttt cttggatgaa agactcaaca
     8221 atcccttcaa cctcattcat ttgctcctga ctagcagcaa aggctccaaa aatcacctca
     8281 gtatccttat tttcagcgac aaaggacaag acttgaagca gagcttgatt atcaatcccc
     8341 tgctcaaaat ccagttgaaa ataaatgatg gattctttct tagtttggct tcgtcccaat
     8401 cgtaatcggg tgtcaaaaga tgataaatga taacattgat gcattcgttc aggatacaac
     8461 tcctgcaaca atcgtaaact atcctctcta tccaccaaaa tcaaatccga tttttcaaaa
     8521 gttacatcta aatccctaaa ggtatcttga ggattacgtc caatgaacag actcaacagt
     8581 ttaggattag tactaggtaa acgatctagt accaactggt catgatgaga atgagaagga
     8641 agcataaaaa tatcctgatc cttcacatac gtttgcagat aattctcaaa aaattctgcc
     8701 accagatctc ccatattttg ataggtaagc attttaaagc gataacccaa aattggattg
     8761 acttctacct gtcctccttc ttttaaacgc tctctaaatt gccaaactcc cttaggattc
     8821 aaatattctt gataggctgc ttgcccatct tcaaaaaaaa taatactcga tataaaccct
     8881 cgatcatcca tgatatggtg catatgtatt tgaccatttg gttcaaaata ctgtatatca
     8941 ctgatgaagc cttcaactcc atgttcaacc ttagcaaatt tcttcccatt tttttgaacg
     9001 ataatcgtaa agggactata aataaattca cagtcatcat cccactcaat atctctaatg
     9061 cttaaaactt gggtatggag attatgaaaa tcttgcataa catcaaaaac ggaataagta
     9121 tccgtttcta acacaccatg tctatataaa aaataacgta gatgaggctg gtaagctaat
     9181 actaatagac gagaatctat atcttgctct tgaaagagcc ggatctggtg aaaggtatca
     9241 tcaaactcta gacgaaaatg agaaaaatac catggagtga tatctgcatg ccatgttctt
     9301 tctgacccat accaagctgg aataaaataa tacataaaag tctcctaaat caaaggttta
     9361 taggtctcat ttagccttaa agccttgacc tcgtctctaa tcgtaaaaat catgcctcca
     9421 aacatcataa acaaacctgg aatcattgcc aatcgtaata acttttcatc aaacaaaaca
     9481 aaaagcatgg gaccacctgc cataaccaca ttaaagagac cacctatgac tgagaaacga
     9541 aggaccaatc gattaataaa tcgactagta tccgcacctg gataaatacc ataaatgtat
     9601 tctccagatt ttttcatacg gtctgcaatc tcttctccat tcatcgtaac aaaagcaaaa
     9661 atgatactaa ataaaaataa gaccgaaata taaacataga cccacaaagg ctttccaatc
     9721 ataaattcct ttgataaagc cgctaaccct gaatgattag ggaaaataaa tcccaacaag
     9781 atgaacaaat aagctggtac actaagaaaa ctcatcacat acatataagg catcccacct
     9841 gcaggattca acatgatttc gagataaaaa tagcgtttaa atcgattgtg taagccaatt
     9901 ttattaacag gaaccaaata gcgagctcga tacataaggg caagtaaata agaaaagaca
     9961 agggttaata aagcaagtaa cacaataatc cctgttggaa tgtgtactgt ctgaaatgtt
    10021 tccaaaacat cctgaggaat atttaaaacc atactggata ggagaatgac tatagaacct
    10081 ccaatcccca tactcgcatt taaatctgac aaccaaacaa gaaaaaatgt tcctgctatc
    10141 agcaatattg tattcattag aacaaccaat attgcagaat aggaggattg tactggcagt
    10201 ctcaagctaa ctgccaatga ttgaatgaca gctatcatca aggtcaggta cattttacgg
    10261 cgatcttgta tttctataga cgtagatgtt aaacccaacc gtttagaaaa agaaaacatc
    10321 tgccataaaa tcatagctga catccaaggg gacaatccaa cagaaaaaat tgacaaactt
    10381 cttagatttc cacctgttag ggcggctgag aaggctagat aggctgttga acctcctaaa
    10441 aaatctttag tatttaggtc aacaaaaggg agagtaatac gacttcccaa tacatagata
    10501 aacagtagaa ataaggtaaa tagtcctttt tttactgcta ttgacgaata aatcttagtc
    10561 ataaaacact cccctcacct tcattgtttg tgatatggta aataacaatt cttttatttc
    10621 aacttttacc atttctttgt tctgcatcaa aaaacaactt taatctgaaa caaatatctt
    10681 tatattcctc agtatctgtt aattgatggt attctaaagt ttgcttataa tcttttaaaa
    10741 gattgacaaa tcgaatacgc agaacggaga catccaaacc agccaagaca caatccgata
    10801 ttttcttaga aaacacttca acaatatctc ttgcccagga aagatcaaat tccgttctca
    10861 tgatgcttcc tgaacgtctt ctatacagat agagattatc gtttataaag acgattttag
    10921 aggctaaaag ataaaagcga tgcatagttg cttcatcatc aaagcggcga ccatttgaaa
    10981 agtgaacttc attgaataat tcttttttaa tcaacttaaa tgtcggcaat ataaaggccg
    11041 agctattgaa tttccaatct cctgcttgac ggttcataat ttcttgtata gctaattctt
    11101 cgacgcaaaa atcttgccct gttacataaa aataaaaatt cccgtcactt tcgtcataat
    11161 tatagtaatt ccctataaca acatctgcat ccttttcttg gacttgctga taaagagttt
    11221 caatcatatt ttcttttacg atatcatctg aatcaacaat cgtgatgtac tctcccttag
    11281 ctacactaat ccccatattt ttagcttgtg ctgccccccc cccttatttt cttgatgcaa
    11341 tactcgcaca cgattatctt gcatcataaa agcatcacaa atttccccag aattatccgt
    11401 agaaccgtca tcaactaaaa taatttctat attttgatag gtttgcttca gaatatgctc
    11461 gacacactcc actaaatatt ttcccgtatt gtatatcggt accacaacac taattaattc
    11521 actcattact ctatctctat attcttcatt ctatccacta aatcatctgg tctctccaca
    11581 gaaaaaatac cgtcatagag tccatcatcc atacttttac gtgtgatatc aaaagcgaag
    11641 attttcttgc cactttcttg tgcccttgta actacattaa aaacctctcc acctgtatta
    11701 atatctaaat aaactataga atcgtccaat agccaatcaa ttctactatg taatacattt
    11761 tgatatactg ttacattcgt atactgtgat agagaggtca gctcctcaga acaatcacac
    11821 ggtgcagcca aatgaaaatg ccaatcaggt aaccgttgta ctaaatactc taaatgtttt
    11881 atatctgcac tccatgtcac aagcatcact tgttttttag actgattgct tctttcaaaa
    11941 taatttaaat cggaacgttg aaaagcaatc tctgaccaat ccaaatctct ataaacccac
    12001 cataattcac gtagtctaga tatactatat gtattccaag gtttatcatg actagcatag
    12061 tgaacaattg taggtggatt gtcatctaga cgttcacatt cttgagcaag gtgataaata
    12121 tcaacaccca ccatataatt atatgtttta tctaaggcta gccaattatc ctcaaaataa
    12181 atatttaaaa tactctgatc cccaagatga acaacttgat tttgctcggc cgctaattcc
    12241 aataaactat tgacaataga gtgttctttc cattttgcaa catccattag taacatacca
    12301 gcattaaatc cagattttcg tccttcatag gcatagacat catcaacagc accaattgaa
    12361 tatcctttga gatctatctc aaacaaagta gctagttccc cagttacaat gatatcggaa
    12421 tccagataca ataccctatc agattcaacc acttctgtcg caaaaaatct aaagtaagaa
    12481 gcataattta tatgaggtcc tgttttatag ctttcaaaaa gttctttatc aatgtgtaca
    12541 ctgcgaattg tagaattcac aacttccatt tttctcccca ataatttaaa ccattcagga
    12601 gctatatcac tgttgagaat ataaaaatca acatctctat tgtgatacaa tacagacttt
    12661 atagtcgtct ctaaaggaat aagataggca ttatctgccg ctaatactat tgattttctc
    12721 atactgttct ctacctcatg taagatctga ttgctgcaac catttttttc ggaatggagc
    12781 ttggatagat accttcatat gaaatctcag aaagattttc agagcaagta ttgtcgaaag
    12841 ctatcattgg ttttttgtac ttaagcacaa actcatatgc atcttctaat tttcgatcat
    12901 gattcaaatc caaatacaaa tctgaagcta ttactatttg ttccaataac attggtaagg
    12961 aatttggata tacggtaaca ttaggaaaac gcgttaaagc tagtagttca tttgccatat
    13021 ttgtataagc aacaatatga aaatgaactt ctggcaattg ctccgccaaa taatcgattt
    13081 tctcgacaca ccaagaattc gttaaattaa cacattgtaa cttaaatatt tgtgatttac
    13141 taggatactt cacactcttt gaaaaccatt catttaaaat aacagaccaa tccatcaaag
    13201 agtattccca ccaaacttct cttagacgtc caacagaaaa ttgattccaa ggcttatcct
    13261 gagaaatata gtgtaaaatt agtggcagtg gttcgagcgg aatatcaaaa atgaattgat
    13321 gtttaaaggc tgccgcccca taatcatatc ctatttgaaa attatattga tcttcaaggg
    13381 aactatattg atctttaaac aacatattca aaattgactg gtctccttct tccacattct
    13441 catgttcttt ttctgttaag tcaatcaatt tttgtcgaat agtttcagat ccccattttt
    13501 tgttgttaat caagagaaca ccagcattga agccgactcc tgctccaaag caagaacgag
    13561 ctgctgccaa ataattttca cctaagtcta attcaaacaa atcggtcaaa tcaccagtca
    13621 caatcaaatc actatctaga tagagaactt tatcttctgt tacaaaatct ggaataaaat
    13681 agcgtgcaaa tgtcatatga ttgatatgag gtaatttatt agaccaattc atttgaaact
    13741 gtgagccaat taacttgcag tcaatcaagt cgccccccat ctcttgtaaa tatattctta
    13801 tttgactaaa ccattcctga ggaatgtcct gatttagcag ataaattttc aaatgactat
    13861 tgtgtctaca gagtgacttc atcgccgttt cgatttgtcg aatataagcg taatcacctg
    13921 caaatgctac agcgcgtttg gtatttctca ttttaaatcc ttatcattta ttttaaatag
    13981 tctagtttaa tattcgtgca acaatctgac tgttataggt ttcatcatca ataaattcaa
    14041 tatcttgatc aaaattatac tctgcagtat gtttatgaga aaatttaacc ttagttttag
    14101 ctcccatttt catccattca tactctgagt caatatggcc tacatccaaa acttgatagc
    14161 ccatctggca tagattataa ctcagaactt ttgctgtagg acccagcata cataaaatca
    14221 agcgaccacc agcatacttt tcaatttctt gttcaatttc atgaactcta gaaaaggcac
    14281 tatgagaagg acagataatt cgcttaatag aatttgcctc atcgaataaa tcatttccga
    14341 cacctgaacg agaagtcgca ccttcgacta tcagtaagtc acggttttcc caaatgcttt
    14401 tcaatttttc aaattgagct ttagcttgac tcttgtcttc aaaatcgata taagggcgag
    14461 atacaaaggt tgagccatac catgaatcac tgcataactc tctgtaaaaa tccatataat
    14521 gatccatgtg atctttccaa aatggaatcg cccacgatgt aaacctaaaa cgatctgtaa
    14581 aagcatcagg aaggcacact actaaatctt ctcgactttc ttggccgata atgtccctca
    14641 tgattgaaac caactcttca tcataatcct ggtagggaat tgaatgccct gcaagcatat
    14701 tgatttcccc atctccaaaa cgaactaaag aagagttgtg ttcaataata taatccaagg
    14761 tttcatctat ccctttcacc tggattatat caagcggttt ttctagcata tccttagtta
    14821 tcaatttttg caaatcagcc aacattcgtt caggatgttc acttgaatac aacctttggc
    14881 cattattccc attttgagat ttataaaagg ctagtatagg tttagataga tgagccattt
    14941 ctgcaacgat atcggatgtt gaagtcagta ggttgatatc taaataagca tccatcttct
    15001 ccttcaactc gtctagctta tctctagaaa ttgccggata gagctgaata ttaggataac
    15061 gattagacaa atcatataat tttggcccca tagctgtcca agcagcaact tgaaaaacaa
    15121 catttggcaa ctgtccagcc aagtaatcca attgttcgat atcctgagaa gccgtaaacg
    15181 ttaaacaagt taaacttgga agttgtcgaa aacaggtaag atgtccgttt ttcttaaaat
    15241 ctttaatcgt atcaatcata aaggaaactt tcccattttc gaaaacgatt tgtcctaact
    15301 ctccgtgctg agtcgattga aaagctagta gcgttttttc ttgttcttgc aaagatttta
    15361 aaaagttctc atctgcacta ccatgattga tatccaaata tagatttgtt gactttttca
    15421 gcttatctaa tacaggagga acaatttgtg gatgcaatct cacattatca tacacagcta
    15481 attttatcag cttatcaccc atatccgtcc aagcagcaat atgaaatacc acatctggta
    15541 aagctgtgac taactcttct attccttcta aatcttggga atttgtcaaa gttaaacaag
    15601 aaaattcctt atctaaagac gatgtcagtt caaactcccc tatatgatgt tgtaaaattt
    15661 tagtccattc caaatcatga aattcccacc ataaatcacg ataacgattg gctatcaagg
    15721 tggtccaggg ttttctgtaa gacacaaaat gaataatact aggaggtact aactcattaa
    15781 aataattttc ccatttatta taaaaagcag ttacatcatg acccacttga aggttaaatt
    15841 ctttatccag ctctaaccaa tcatcttgta agacctgatt aaaaatcgtt tgatcaccat
    15901 taaaatgctc gaaacggcct tcttcaactt ccttcattgt aacaatgctc tgttcaatta
    15961 gtcgttcttt taatttctct tgacgccatt ttttattgtt gattaataaa acacctgcat
    16021 taaatgtaat tccatctgta tctttaactg ctgcgagtga tttttcttct aaacaaatac
    16081 taaataattt ctctaaagaa gtatttacaa tcaaatcact gtctaaatat aaaaccttat
    16141 cttcttggat ataatctgca ataaaatatc tagcataggt aatgctacta atgtgatctt
    16201 gcttttccca atcttgaaac acagtttgtt caggtagctt cacatcgata atctcactac
    16261 ctaacatgcg agctattttt cgtggtttgc gaaaccaatc tggcataata tcttgattta
    16321 aaatataaat cttaacatct cgattgtggt ataaaataga ttttatggtt gtttctaact
    16381 gcctgatgta attacgatca ccagctaata caattgtttt aatcatcttg ttctcttatt
    16441 caactcccaa atggtaaatt gcatctacca ataatttttt agtaaaataa ccttctttta
    16501 gcaaataact aaaggttttt atccgattcg tcatttcttg atattcttgt agattcattt
    16561 tatcaactat cgcatgaact tcttctagac tattcgccac aaagcctaag ccttgatcta
    16621 ctataaattt agcagttgac aagctacttg gtacaatgac tgggatgccc gctgtcagat
    16681 aggtactcac cttatgagat atatttaagg tataatattg gttgctttct ccatcgtttt
    16741 gataagttcc ccagacaagg ccaaatccac cctttgataa ttctagcaac aattcctcat
    16801 cttttttcca tccttcgatg ctgagatttc tagcactaga actagcttcc cctttatttg
    16861 aaaatactct caaaggcgta tcttgagacc aattttgtaa gtctggaaaa cgctctaaac
    16921 ttccagcaaa aaaaagttct tttttaaaag caggggtgta taaggataaa tcatgaggat
    16981 gatcccacat cccttgaaca agaatcttct tagtcgtcaa tccttcttcc atcaggcgtg
    17041 ttttcattct ctctgacggc actatcaaaa catctgatag attatacatg tacatatagt
    17101 ctttcatgag ataatagtta ctatcaaaca tgaggggaac aacatcatgg ataaagcaaa
    17161 taattttcac ctgcatatcc ttcaacttat caaacaagag acgatcaaat tcaaagccat
    17221 tccaggttgg agactgaaag actaaaatat ccccaataga aatactggcc ataataccat
    17281 ccagacgctt attcatttca gaaggactat ctgaagcaat gttgtaaaaa taaataccaa
    17341 cctctctaaa tcccagttga ctagctatct tttgaacagc attttgagct aagataaccg
    17401 tactatcacc agccatgccg tataaatttg ttaaatgtag tttcatatta cgctatctca
    17461 ttttttatta ctatctacgg aagtaaagac aatttttcca tcattcttct ataaatctct
    17521 gtgaactgca tattttgttc ttctaatttt gctatcactc tttgtaatct atttttataa
    17581 atcaaaatat gattggtcaa gtcatagtct gaacttgcta aaatagctat cttttcttcc
    17641 ctagccccga tttcatcata catacgcttt tcactccatg tattcgataa agtatcagat
    17701 aaaccaacac ggtaccagta aacacaacga ttcaaataga ctatcctcga agctcttaga
    17761 agtaccttcc atgtccagta agtatcctct gcaatttttc ctataggaaa tggtaaatct
    17821 tgtagtaact ctctcttgaa taacttcaag acagctacag tccagttccc atttctgact
    17881 tcttcgacac cttccctatc catgattgct ttaccttcta tcacttccag agaatcatct
    17941 ggatccgtaa cataagtcat atacacatag cgtgtttcat cataagaatt ataacgcccg
    18001 atactaatat ctgcgttttc ctttttcaaa gcaccatata atcggtctaa agcatcatgt
    18061 tccaaccaat catcagagtc tacaaaagta atgtacgccc cccccgaaca ttcaatacct
    18121 aggttacgag ctgatgaaag accgccgttt gctttctcaa aatatttgaa acgagaatct
    18181 ttctctacaa attcttcaca tattttggat gaatgatctg gagagccatc attaaccatg
    18241 atacattcaa aattcggata agtttgtttt tgaatactat ccaaacactg tcgcaaataa
    18301 ttctcaacgt tgtagattgg gactacaata ctaattagtt cattttccat atactaactt
    18361 tcctagactt gatttattct atttccctct taatctcttc tctttacaac aagatttcca
    18421 ttattataaa ctacactgtt tgatggaata tcattgcgta tgagacaacc tgctccaata
    18481 atggtattat ccccaatcgt cactcctttt aaaatcgtga cattacttcc tatccagcag
    18541 tctctgccaa ctctaatcgg cgctgtagtc cactgccact tttcaatctt ttcagcagta
    18601 taaatatggt cgtgatcata aaaacgaaca ccctctccca tcatggtacc agctccgatt
    18661 tcaattcttt ccatacagtt aaaactacaa gagttgttga taaaaacacc atcatgcagt
    18721 attaacttac cagaagagac atgaaaattt tcaaaatttc tgcagatgac atttgctcct
    18781 acaatgaagt gagccttatc atcgatttta aagacattcc aagtcccatc ttggatgatt
    18841 ttttcttgat gattggtgcc tactctgact tcattcagat agtctctcac tctagaaacc
    18901 aatttgtctg gtgtacttga tagaaaaact tcctgcccct gatttccatg gactgtattg
    18961 tcaaaagcaa agacagattt tccagcctgt acaaacttgg agacaatagc atctacttct
    19021 tgaaagtgat tgatatctag taaaaagtca caggtatcga ctagaaagtt tagctgttct
    19081 tcatttgtaa tcgttcggag acggacatta tagttcgttt ctaagctacg gattttctcg
    19141 gaaaattgaa caggtgctgc aatataaaaa cagatatttg gcaaggattc aaggagataa
    19201 tccaactctt caatctgatc actaacagtc aaaatcagag catctcctac taaatgattt
    19261 ttcttcattt gcttatttct ctcaatttct caatcattgc ttgaacttgg tcaacagcat
    19321 atacctcctg acctgcttca taggttttgg tattttcaaa ggacaaaata gtttttccaa
    19381 gtcttgcaaa ttgattgata atttcctctg ttttttcacc atgattaata tctaaaagta
    19441 cttgactggt ttctaccaat tcattatcga catctaccaa atagtgaatt ccgttaaata
    19501 tagtcacgtt tggataaatt gtcatctgag ccaatcgatc actaactatt actctagctg
    19561 ctatcttaaa ctgaatatca ggcaaggatt gaaccaatgt ctcaatttgt tcaatatggt
    19621 ctgaggcagt atagattaga caagtgaaag gttcctttat tggatagatg tgagatcttt
    19681 gtaatggatg taaatgatgg ttttgtccca attctgtcca ttcaagccca tgatagtacc
    19741 accaaacttc acgataggtt tgggccgcca aatctttcca cggtttccga tgagaaagat
    19801 agtgaataat agcaggataa tcctgaccct caggcaattg ataatcagca aactgtttat
    19861 gaatgacaat atgattataa tcaaagtcca attccaacca tttatgttca aaaagcatat
    19921 tcaagatgct ctgatctgcc tgatccacct tatcatgcca ttcattggtt acatcaatta
    19981 atttttgggt catattctct tttttccaaa aagcattgtt taccaagaga acaccggcat
    20041 taaagatttc ttgaccaaaa taagctctgc ccccaaaatc tctaacagca gccaaaggat
    20101 aatcttgtaa gtctgtagca aacaaatcat caagattttt cgttacaagt aaatcacagt
    20161 ccaagtagag ggccttatct tcttgcacga aatcagctat gaaatagcgt aaaaaaactg
    20221 tgtaactaat atccgtttta taacatgaaa tttgctcaga agttacccga caattaataa
    20281 tttctgagtc aaacttctct aagcgcttat ttaattgctt aatccattca tttggaaaat
    20341 cgctatgaat cagataaaaa cgaatcgaac gattatgata acaaatagac ttgattgtcg
    20401 ttaaaacttg gtctacatag ccatagtttg ctgctaggac aatggctttc ttttcacttg
    20461 attcttgtac cgttaactga tttaaaagtc tttttttcat ttcaaactct ttatatattg
    20521 ctgtgtcaga taaaccacta gcttgaccgt tggcgagact gacttccaac atctgacgat
    20581 aaactgccaa gtgtctctct agaggatatc ccatattagc tagtagcgta atacgttcag
    20641 acatagcatc aactaaggcg tgcatccact tttctgtcca aactcttgat agactacctt
    20701 ttctaatccg ataagcataa agacctttat ttaaataaat taccttttcc gataataaat
    20761 ataccttttg attgaggtaa ccatcttctc ctaatttacc catgtcaaag cgcaactgct
    20821 caaacaatct tgccttatag agtttaccac aagcagatat caaagcaaaa ctcttcattt
    20881 cttgagtttc ataacaagtt ctcaaagatg gaaacattgt catatacctt atcataatag
    20941 gagtctcctg atatatgaaa gtagaacatt ccttcacttt cgttgaaaga atagtaattc
    21001 ccaactgcaa tatcagcttg atattctgtt atttttttat atagggtttc tacataatct
    21061 aactcaatcc aatcatctga atccacaaag gtcacataat ttccggacat attattcaga
    21121 ccggtgtttc gtgcggcaga aataccagca ttttcttgtt ctatatagag aattcggtga
    21181 tccatttctg aaaattcttt acaaatttca cctgaagcat ccgtagaacc atcattaacg
    21241 acaacaatct caatattttt atatgtttga gaaataatac tatctagaca tttcctcaga
    21301 tagttttcta cattgtatac tggtacaatg accgtaattt tatcatccac tagcaatttt
    21361 ctccttgatt tatgtgcgcg agttagttta gcctaacttt acaggttgtc ttactcttct
    21421 tcatcacgtt tacggcgttt cgcaaccaat ccaatacctg taacagctgc tagaactcca
    21481 agtaacacag atccaattga cgactcagta cctgtattag gcaattcttt ttgggattta
    21541 cctgtcgatc cagaagcatt tccaacttgt gagttcgaat ggtttgctga atttgaaact
    21601 gatgtacttg ctgaggctga cgcactcgtt gatgctgact cagacgcact ggttgatgcc
    21661 gattcagacg ctgaggtgct tgctgatgct gaggcactgg ttgacgctga agctgaggcg
    21721 ctagtacttg ctgaagccga ggcactggtt gatgctgagg ctgaggcgct agtacttgct
    21781 gaagctgagg cacttgtcga tgctgaggcc gaagcactgg tacttgctga agccgaagca
    21841 ctcgttgatg ctgaggctga agcactggtt gacgctgaag ctgaggcact cgttgaggct
    21901 gaagccgaag cactcgttga tgcggaagct gacgcactgg tacttgctga agctgaggcg
    21961 cttgtgcttg ctgaggccga ggcacttgtt gaggccgatt cagaagctga ggtactcgtt
    22021 gatgcggatt cagatgctga ggtgcttgct gaggccgagg cgctggttga tgcagaagct
    22081 gaggcactgg ttgaggccga ttcagaagct gaggtactgg tgcttgctga agctgaggca
    22141 ctcgttgatg ccgattcaga cgctgaggtg cttgctgaag ctgatgcact tgttgatgct
    22201 gaggctgagg cgctggttga tgccgattca gacgctgaga tacttgctga ggctgaggcg
    22261 cttgtacttg ctgactctga tgcactggta cttgctgagg ctgaggcact tgtgcttgct
    22321 gaagcggacg cactcgttga tgc
//
LOCUS       DAVBCN010000028        22195 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVBCN010000028 DAVBCN010000000
VERSION     DAVBCN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22195
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.028"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(70..2028)
                     /gene="ftsH"
                     /locus_tag="VQ497_001012"
     CDS             complement(70..2028)
                     /gene="ftsH"
                     /locus_tag="VQ497_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET2517336.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEETGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            complement(2044..2586)
                     /gene="hpt"
                     /locus_tag="VQ497_001013"
     CDS             complement(2044..2586)
                     /gene="hpt"
                     /locus_tag="VQ497_001013"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET2517337.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            complement(2590..3867)
                     /gene="tilS"
                     /locus_tag="VQ497_001014"
     CDS             complement(2590..3867)
                     /gene="tilS"
                     /locus_tag="VQ497_001014"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET2517338.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETIFMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIKNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            complement(3864..5132)
                     /locus_tag="VQ497_001015"
     CDS             complement(3864..5132)
                     /locus_tag="VQ497_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HET2517339.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            complement(5252..5620)
                     /locus_tag="VQ497_001016"
     CDS             complement(5252..5620)
                     /locus_tag="VQ497_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET2517340.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            complement(5613..5879)
                     /locus_tag="VQ497_001017"
     CDS             complement(5613..5879)
                     /locus_tag="VQ497_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET2517341.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            complement(5937..9446)
                     /gene="mfd"
                     /locus_tag="VQ497_001018"
     CDS             complement(5937..9446)
                     /gene="mfd"
                     /locus_tag="VQ497_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET2517342.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYAQHAFIHQLKENG
                     YQKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERELAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPIQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKESKEKNSI"
     gene            complement(9447..10016)
                     /locus_tag="VQ497_001019"
     CDS             complement(9447..10016)
                     /locus_tag="VQ497_001019"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET2517343.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVKYYLQEKNFEKTMQRYNG"
     gene            complement(10087..11202)
                     /gene="ychF"
                     /locus_tag="VQ497_001020"
     CDS             complement(10087..11202)
                     /gene="ychF"
                     /locus_tag="VQ497_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET2517344.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIIEFRFNV"
     gene            complement(11286..11480)
                     /locus_tag="VQ497_001021"
     CDS             complement(11286..11480)
                     /locus_tag="VQ497_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HET2517345.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            complement(11545..12681)
                     /gene="dnaN"
                     /locus_tag="VQ497_001022"
     CDS             complement(11545..12681)
                     /gene="dnaN"
                     /locus_tag="VQ497_001022"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET2517346.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            complement(12840..14201)
                     /gene="dnaA"
                     /locus_tag="VQ497_001023"
     CDS             complement(12840..14201)
                     /gene="dnaA"
                     /locus_tag="VQ497_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET2517347.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVSMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            complement(14414..15172)
                     /locus_tag="VQ497_001024"
     CDS             complement(14414..15172)
                     /locus_tag="VQ497_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET2517348.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            complement(15230..16411)
                     /locus_tag="VQ497_001025"
     CDS             complement(15230..16411)
                     /locus_tag="VQ497_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET2517349.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVIVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            16594..17073
                     /gene="rlmH"
                     /locus_tag="VQ497_001026"
     CDS             16594..17073
                     /gene="rlmH"
                     /locus_tag="VQ497_001026"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET2517350.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            17117..17190
                     /locus_tag="VQ497_001027"
     tRNA            17117..17190
                     /locus_tag="VQ497_001027"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17151..17153,aa:Arg,seq:tct)
     gene            17355..17480
                     /gene="comC"
                     /locus_tag="VQ497_001028"
     CDS             17355..17480
                     /gene="comC"
                     /locus_tag="VQ497_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HET2517351.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRISRIILDFLFLRKK"
     gene            17501..18815
                     /locus_tag="VQ497_001029"
                     /pseudo
     CDS             17501..18815
                     /locus_tag="VQ497_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            18812..19564
                     /locus_tag="VQ497_001030"
     CDS             18812..19564
                     /locus_tag="VQ497_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2517352.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            19616..19687
                     /locus_tag="VQ497_001031"
     tRNA            19616..19687
                     /locus_tag="VQ497_001031"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19649..19651,aa:Glu,seq:ttc)
     gene            19693..19766
                     /locus_tag="VQ497_001032"
     tRNA            19693..19766
                     /locus_tag="VQ497_001032"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19727..19729,aa:Asn,seq:gtt)
     gene            complement(19807..20349)
                     /locus_tag="VQ497_001033"
     CDS             complement(19807..20349)
                     /locus_tag="VQ497_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET2517353.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            20528..21295
                     /locus_tag="VQ497_001034"
     CDS             20528..21295
                     /locus_tag="VQ497_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HET2517354.1"
                     /translation="MIGISLIPDLYNIIFLSSMWDPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            21380..22060
                     /locus_tag="VQ497_001035"
     CDS             21380..22060
                     /locus_tag="VQ497_001035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HET2517355.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFVAAISTNMIFAKLPSGRH
                     PESRWAWLKSRAEINGIIAVLAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMV
                     TTLTTWNSRIGAFFLLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQT
                     ISINKSFS"
BASE COUNT         6868 a         4086 c         3621 g         7620 t
ORIGIN      
        1 cattttctcc attctgttcc ctttagctcc taaactgcac aaaaagagag gctccagcct
       61 ctctcagggt tatttttcgt cattcatttt tgactttact tcatcatagg atagtgcatg
      121 agattcctct tctactgctt caggcatctt tcctgtttcg taaagagctt taatttgtgt
      181 actatccaat gtttcgtatt tcaataatgc ttctgcaatt aacttgtgag tttcacgatt
      241 tgactgaata atttcagcag ctttatttcg tgcctcattt aataatgaac gaacctcttc
      301 atcaatttca taagctgttt gttctgaaat tgatttttga ggactctgtg caccaagcat
      361 agcatggttt ccttcatatt gtactgggcc aagtttttca ctcataccgt actctgtaac
      421 cattgcacgt gccatttgtg tcgcttgttc aaagtcgttt gaagctcctg tagtttggac
      481 attaaagata atttcttcag ctacacgtcc acccattaag ccagccaatt gctctttcat
      541 atcttcttta gatagaagca tttgatcctc tttaggaagt gcaatcatgt atccgcctgc
      601 acggccgcgt ggtacaattg taaccttatg gacaacgcga gcattcgaca agactagacc
      661 aacaatggta tgtcctgcct catggtaagc aaccaattct cgttcttttt gtgaaactgt
      721 cttatctttc ttagaaggtc cagcaataac tctatcttct gcttcatcaa tatctgaagc
      781 atcaattatc gatttattgc gacgagcagc aactaaagct gcttcattca agacattctc
      841 taaatcagca ccaacaaagc ctggagtttg ttgagccact aatttcaaat caacatcttc
      901 tgctaaaggc ttgttcttag cgtgaacttt caagattgct tcacgacctt taacatcagg
      961 acggccaacc aatacttttc tatcaaaacg tcctggacgc aaaagggcag gatcaagtac
     1021 atctgaacgg tttgtcgcag cgatgacgat aatcccttca tttccctcaa aaccatccat
     1081 ctcaatcaaa agttggttca aggtttgttc acgttcgtca ttacctccgc cgagaccgac
     1141 tccacgttga cgtccaacag catcaatttc atcgataaag atgatagctg gtgctgcttt
     1201 tttggcatcc tcaaaaagag agcgaacacg actagctcca actccgacaa acatttctac
     1261 aaagtcagaa cctgagatac taaagaatgg aacacctgct tctccagcga ctgccttagc
     1321 aagcaaagtt ttacctgtcc ccggaggtcc ctccaaaaga acacctgctg gaatacgggc
     1381 tccaagtttt gtgaatcgtt ttggatcttt taagaactca acaacttcaa ctagttcttg
     1441 tttttcttcc tcagctccag caacatctga aaatcttact ttaatatctt ctttatttgc
     1501 tgctttagcc ttactacgtc caaaactcat tggattacgg ccattgcctc ctcccatatt
     1561 tcccatcata gagaatagga agaagaatag aattccaaat ggcacaatgg atacgagtag
     1621 attaatccat ataccacttg aactttcatg cttaacagtt acttctgctt tatggtcagt
     1681 agcaagtttt tgcaattctg atacggtagt atctgcagga agaatagtgc tggtaaattt
     1741 ctctacctta gtaacagatg gcgtgaaaaa ctgaatacct gtttcttctt tacttgtttt
     1801 aggattttta tagacaccag aaacttcgat aacactacca tttggttggt aagttaattc
     1861 ttttacatta ccatcggtaa tttcttgtac caactcagta tagttgattt gctgacttcc
     1921 tcctgagtta ttcccagaat agaaatactg gaatcctgtc acaaggaaaa agataaataa
     1981 taaccataga aaaggatttt taattaaacc attattttgt tttttcatta aagattattc
     2041 tttctaattt gaatacactt cctctttcaa tactccaata taaggaagat tacgataatt
     2101 ttctttgtag tctaaaccat aacctactac aaactcattt gggatagtaa agcaggtata
     2161 gtctgcctca atttctacaa cacgtccttc tggtttatcc aacaaggttg caattttaac
     2221 agaagctgct tctctttctt taaacatatc tcgcaaattc ttcaaagttt gacctgtatc
     2281 aatgatatct tctacaaata gaacatgtct tcctttgata tcttgagtca catcttgttt
     2341 aatattgata acaccactac ttgctgttcc accatggtag ctagaaacca tcatgaagtc
     2401 catttcaata tgtgtatcaa tatgtttgac caattcagcc ataaaaggaa tagatccttt
     2461 taaaatccca actaagattg gattttttcc tgcataatct ttagttaatt gagcacctag
     2521 ttttttagct gcttctgtaa tttcatcgtg tgaaacgagg acttttttaa tatcgttttc
     2581 taacattttt tacctatcta ttttttctat ataaagtaca gtgttcatta tatcattttt
     2641 cgttttttta ctcaaattat tggtcgcaat tcccaaaatt gagacaattt caccaaattg
     2701 ctcaataata agagcagagt ttctcttttc catagggatt ttcaaatcaa taaataaacg
     2761 tctgagtttt tttctatgcc catttttaat caaaacatct cctgtttttc gatgacgaat
     2821 gtgtatggat gtttcacgtg aaacaggtat ttgttgaatt aattcacctt ctaatggaag
     2881 tccaaaagaa aataaatatc cttgataagc tacctgattt tgatagtgta acacaagttc
     2941 atcttccttt tcatcagcct gcggactgat tttacaaatc tgaaactgtt ggtactcttt
     3001 tatcaattca tagccatttt taatcggatg acgatactgg cttttagatt ttaaaatctg
     3061 ctgaacttca gcaaactgag cttttgtaag attcaaatct ggaaaacgat tcagataagt
     3121 ttgaagtaaa actctttgtg tagactcaga gtaagaaaat aactgctgta aatcttccac
     3181 attaatattg ttagataatt cagctattgc caaatcataa tctaaaattt cattgccaat
     3241 gcctaagatt gcatccctaa atcgaggatt ttctttttcc aattctggta agtaagaatt
     3301 tcgaatacga tttcgaaaat aatgattctc ctgatttgat gtatcttcaa agtgaaaaat
     3361 tgatggaaag tcttttttct gaaaatgcaa gaagggacga atgatttcta tctctccgac
     3421 tacttgcttc tccttaattc ctgatagata gcgcaagcga gttcctcgaa tcaagcgcat
     3481 aaaaatcgtt tccacctgat catcagcatg gtgggcagtg actaaagctg tcgcacctgt
     3541 ctttttcatg acctcttgaa aaaaatcata acgaaaattt cgtgcacgcg cttctgaaaa
     3601 ttctcctgaa aaattgctga tataaatagg aagctctgct tcagcagcca acttccttaa
     3661 ttccttttct tcccaatctg attcaattct ctgcttatga ttcacatgag ctagaatcaa
     3721 ttcaatctct aactcttttt gataagtaga caatacctta aatagaaaca tggaatctaa
     3781 tccaccagaa agagctagaa ccgccttagc atgctttttg aaatatccct tcttgagaaa
     3841 atgatttaaa aaatctggtt ccctcatttt agaacctcat aaacatcctt ggctatctta
     3901 gaaatcgtat cataatcaga attcttagtg aaaatagaaa gaataaatgg agaatctgca
     3961 tagacaacac ccgtatcatg cttaaattca tccgcatctc caattttatg agctacttta
     4021 acagaaacac ctttggcaat tcgctgacta tcaaaatctg ttttagtcaa agactctagc
     4081 acaaatccat tttgattata aatagcttcc ataaacttcc cggccatctt agaagaaatc
     4141 aatttttctt ttggatccca atcatctccc ataatggcag acatcttgga tttgaatgtg
     4201 gcatcagatt ggtttgaaat gtaatatccc aatagattat gagctacatt atcagattct
     4261 tttgatactt tcgtaattaa atcctttaaa gaatattctt tattatcttc ttttttagga
     4321 agactaccac ttccctctgg tttataagaa cctggaaaat cattgactgc agatacgtat
     4381 tttacagtcg tatctaactg ataaagaccc tcatttattt tttcttgcgt ataatagaga
     4441 taagagagtt tcaaaacgct ggctgcatac atcttttcat cttgattgat accagcttct
     4501 tttccagtag tcagttgctt aacataaata gagaaagaat ctttctgata tttttcagat
     4561 aacatttctt gaactttact catccgatta tcttcttcag aagttgattc tttagctacc
     4621 catccagcct gatcaatatg tagaaattct cttccttcta caaacatggt cttgtcgatt
     4681 gatacttgcg aataagctga taaggatgat ttcacttctt ttaaatcata aggactattg
     4741 tacagtttaa agtcagattc taaccatact ttttttattg ttggagttac ctctgattga
     4801 tcatataaaa atcgtttgtc cgcagctata aattgatgat ttgatagctt aaatactgga
     4861 attccttgtt tatttaagcg ccactcggtt atttgaaaac ttgttttggg agtcaatttc
     4921 ccagattcca ctactaaatc ttcattcgca taaacaggaa cctctccata aaccatggga
     4981 gaacttaatt tttctctaaa ataaatacca aagtcagatt gtgaaaggta ataaatttct
     5041 ttcgaagtat agacgacttc tttttctgtg ctaacgactt ttgaaatggt caaaaaactt
     5101 ggtagcaaca aaataattaa gaatttacgc attcttcctt tccttttctt cttgtaaacg
     5161 caataattga tttttatcag ccaattcttc cagcttttca tctgacaaac tcaaaaattg
     5221 ctctattacg tctaataaat tttccatttt atcacctttg aagcaagtca ggaatcgtat
     5281 aaactttttc cctcgactta gaataatagt acttcgctcg tgtatatttg gcagcataat
     5341 cttcatcttt caacttggta gcaaatgctg tctccttatc cttttcatca ctcaaagttt
     5401 gatactgagt ttgcaagtct gctaattgct gacgtctttg gagtaattgc tgataactct
     5461 gcgctaaatt aaaagttggc aagataaata atagcataat caaaatcaat acccctccca
     5521 taaaacgatt ccgtttttgt cgttctttca tcaggtagcg acgacgttgg tattcatttt
     5581 gaataaaaga attattcaat tgtacaatat ttttagacat tttcttctac ccgtgtttca
     5641 ctgataattt catacattcc tgctgcatct tctttttttg tactatcttt catctctagt
     5701 acttttacaa gcagcaactt attgccaaag cgaatttcaa cttggtcatt aactttcaag
     5761 tccgttgaac ttttggccaa gattccatta accttgattc tacctttatc tgctacttcc
     5821 tttgcgactg tacgacgctt gataattcgc gatactttta aatatttatc taatctcatc
     5881 cttattacct caatttatta ttgtaccatt tttatcttct ttatagaaga aaaatatcaa
     5941 atggaatttt tttccttaga ctcttttatc tctaataaac tttctccaaa aatcagcaga
     6001 ccttctaaaa tttcataatc tttcttattt tggacatcaa atacaagctc cattaatccc
     6061 ttattctcag cgatgcctgc ttttaagttc gttacggata aagctttaaa ataatcttga
     6121 gctaaaaaca gtcgttgagt gactttttca aattgaattg taattttatt atcttttctt
     6181 tccacacgtt gaacaaagac cttgtccaag tatgatttga ccaaaccaat ctctaaaaga
     6241 taggctacta catctgggta ttctccaaaa cggtctatca actcctcttg taactcttca
     6301 taattgacac ggttgtcaat ttgacgaatt ttcttgtaaa tttcaatctt atgtcgttga
     6361 tcagaaatat aagtatcagg aagataggca tcaatttgca aaatcaactc agcattccct
     6421 ttggttcttg tgttagcgtt agcattaccg tttcgtttag caatagcttc ctctaataac
     6481 tgcgaataca attcaaaacc aacagaatca atgaaaccag actgggattt tcctaaaaga
     6541 tttcctgctc cacgaatcga aagatctcgc attgcaatct taaagccaga gcccaattct
     6601 gtaaatcctt taatcgcttc taatctcttt tcagagactt cactgattga tttttctgga
     6661 cgatacatga gataagcata agcaatacga ttactacgac cgactcttcc tcttaactga
     6721 tataaggttg acaagcccat atggtccgca ttttcaataa ataaagtatt agcatttgga
     6781 atgtccaccc ctgtctcaat aatagtagtc gtcaccaaga tatcgtattg tccctcaata
     6841 aagtctaata gagtattttc caactggact tcactcattc gaccatgaac atatccaatc
     6901 gaagcctccg gaatcaactc ctgtaattct gaaaccttct gaacaattgt gtcaactttg
     6961 ttgtaaagat aataaacttg acctccacgc tccatttcac gcaagacagc atcacgaatg
     7021 acactatcat tcttttccaa aacataggtc tgaacaggat agcgattagt cggcggagtt
     7081 tcaataacag ataaatctct gattcccagc atagacatat ggagggtacg agggattggc
     7141 gtagcggtca aggttaggac atccacttgt ttcttcagtt ctttcaaagt ttccttatgc
     7201 ttgacaccaa atcgctgttc ctcatcaata atcatcaagc ccaaatcagc aaacacaaca
     7261 tcttttgaca aaacacgatg tgttccaatc aaaatatcga cttgaccgtt tttcaatttt
     7321 tcaagtgttg cagtctgctc ttttttactt ctaaagcgac tcaacacatc aatattaact
     7381 gcaaaatttt ggaatcgttc cttaaaattc gtatagtgct gttgcgctaa aaccgtcgtc
     7441 ggaactagaa tgacaacctg tttgtgatca ttgactgctt taaaggctgc acgcatagca
     7501 acttcagtct ttccaaaacc aacatcccca actaaaagtc gatccattgg ctgagaagcc
     7561 tgcatatccc tcttgatttc ctcaatacta cgaagttgat catccgtttc aacataaggg
     7621 aaagcatcat caaaggcatc ttgatcatca tcatcagctg agaaagcaaa acccttcaac
     7681 tgactacgtt cagagtagag tttgattaaa tcatcagcta tatcctctac ctggttctta
     7741 accttttgct tggccttttt aaaatgaccg tcatttaatt tattgagttt tggagcttta
     7801 ccatcacttg aaatatattt ggacagtaga tgaatctgtt ccacggggat agaaatttga
     7861 tcaccatttt ggtattggac actgacataa tcgcgatgaa ttcccttgat ttcaatggtt
     7921 tcaattccta gatattgacc aatcccatgg atatgatgga caacatagtc ccctttttca
     7981 agttcattgt aatcttttaa tctctctgca tttgaaacat gttgtcttcg aaaacgacgc
     8041 tttaatttct tttgaaaaat ctcatgttca gttatcaata aaatcttttc atctacaaaa
     8101 tgaaaaccat gtctgagatt accctctatt aaatttacag attctttaca gatacttgtc
     8161 ttatctctag aatccaattt aatctgatat tcctctaaca tatcctccaa tgttttactt
     8221 cccattgaat tgctagactg cagaataatg gtgtaatcca tttttttata tcgttcaatt
     8281 tcttctttta gaaaagaaaa ctgattgaaa aattcctgta taggatattg attgaattga
     8341 taaattttgt caaatttgag atttcctaaa cccttttgaa gattagagaa aaaggtcact
     8401 ggactttgtt ttttatagat ttgttcaata tcagaaaaat actgcatatc agaaaatgct
     8461 ttactattct gtaattcttc tgtaaagtac tgcgctaatt ctctttcaaa gacttcatac
     8521 tgattcatca atttttgata atcatcaaag aatattggag tatctttttc aatataatca
     8581 aagacagtcc atgtcttatc atagcacaaa gataaaaact tccgagaatc tgcatgactt
     8641 tgtttttggt gaaaacttga aagaatttct tctaggtatg atttcaaaat aggtgataaa
     8701 gtttttgaaa tttgtttttc taaagctgac tgtcctcgtt gataatcctt ttctctcaaa
     8761 agcatatcac tagctggaaa gatagtgagt tctgtcttat tttctttcga taattgtgtt
     8821 tctacttcaa atgacctgat accatcaatt tcatcaccaa aaaactcaat tcgacaaggt
     8881 tctaactggg atatttcaaa aatatctaaa atatctcctc gaagactaaa ttcgccctga
     8941 gtttgtactt gagtaacttt ttgatagcca ttttccttta actgatggat aaacgcgtgt
     9001 tgagcatatt cttcaccaac tgagattttt acaatactat ctttgaatgc attgggagac
     9061 ggtaaaatca atcgacttgc tgcgatatta caaactaaaa tccctttctt agatgaatca
     9121 gtcaaaaaac gcaaggcttc aacccgtgaa ataatttttt cctgtgaaga catcaaaaac
     9181 tccaccatag gagcatcatc taccaaaaat ggatagacga gttcctcacc caagatagaa
     9241 ataagatcac taacaagtcc ttctgcttct ccataagttg acgtcaataa cacaatccta
     9301 tcttcttttt ctaaactgct tgcaattgca agagccttag tagatgttga taaaccaagt
     9361 attagttgtc ttttcttatc tgttaaattt tgatgccatt ttttaatctg atcattttct
     9421 gagaataaat ctaataaggt caccatttat ccgttatacc tctgcattgt tttctcaaaa
     9481 tttttctctt gtaaatagta ttttacagaa tcgtcaactt tgtcaacaga ctgtaaaata
     9541 ccgatataat catccctgtc aaacttactc aaaacatgat gaacaactga cataccattt
     9601 ttaggtcttc caattccaat cttaacacgg ttaaagacct gagttcctat atgttgaata
     9661 atagacttga taccattatg accacctgct gagccttttg ctcttaaacg aattttccca
     9721 acttccatgt caagatcatc gtaaatgata agtaaatcgt caatatccaa accatagtaa
     9781 gttaataaag catgaactgc ttttccactt tcattcataa aggtcgttgg tttaaccaga
     9841 taaatttttt ctccatttag gaaaaaggat gctaggtcag cttgaaatat cttatcgtgt
     9901 gtaaaagtga cattctgttt cttcgctagt tgatcaatca acataaaacc aacattgtgt
     9961 tttgtttcaa aatatttatc ccctggattt cccaaaccta caagtaattt ggtcatttat
    10021 ttttcctttc aaaagccaaa agggttggaa tttttttcca acctaattga caccatttat
    10081 taatttttag acattaaagc ggaattctat gatgtcgcca tcttgaacga tatattcttt
    10141 tccttcttca cgcaagcgtc cagcttcttt tacggccttt tcagatccgt atttcactag
    10201 atcttcatat gacatggtta ctgcacgaat aaagcctttt tcaaagtctg agtggataat
    10261 accagctgct tgaggagcct tcataccacg tttgaaagtc caagcgcgaa cttctttttc
    10321 accagctgtg aagtaagttc ccaatccaag caagtggtaa gctgcacgcg tcaacttatc
    10381 tacacctgat tctgtcaaac caatggcttc aagaaactct tttttatctt catcatccaa
    10441 ttcagaaatt tcttcctcag cacgcgcaga aataacgact acttcagcat tttctgtcgc
    10501 tgcaaattca cgaatttgtt tgacatagtc gatagagtca ggttctgaaa ccacatcctc
    10561 gtccacatta gctacataaa gaactggttt agtcgtcaaa aggaaaagac ctttgacaac
    10621 cttttgttcc tcatctgtaa attcaatggt acgagctgat ttcccgtctt ctaggactgg
    10681 tttaatcttt tgaagaacat tgaattctgc tactgattct ttatcttttt gcgtacgtgc
    10741 catcttttct acacgcgcat atcgtttgtt cactgattct aagtcagcaa gaattaattc
    10801 cagattaatt gtatcaatat ctgcaagtgg atctacaaag gcgtcttcac gtccttgctc
    10861 gcgcatcaca ttttcatcat caaaagcacg aactacgtga acaatcgcat ctacttcacg
    10921 aatattggcc aagaatttat tccctagccc ttctcctttt gaagctcctt ttacaatccc
    10981 tgcaatatcc gtaaattcaa atgttgtggg aactgtcttt ttaggagtta tcatttcagt
    11041 tagtttttgt aggcgttcat ctggaacttc caccattcca acatttggat caatcgtcgc
    11101 aaatgggtag tttgctgcct ctgctcctgc ttttgtaatt gcattaaata gtgttgattt
    11161 accaacgttt ggcaaaccaa cgatacctgc tgtcaaagcc atatttctca ttctccgttt
    11221 tcatttcaat ccctaatatt ataacacaaa aagggataaa gtgctaaccc tctaactaag
    11281 ggttctcagt caataatttt attcattttt cgctcaaaat catatcgccc catcatgaca
    11341 acatgctcac aattactgca ttttattttg atatctgctc ctacacgtgt aatttcccaa
    11401 cgattagcct ttttaccagt tgacttgatt gtacaagcgt gtgatttttt catctcaaca
    11461 aaatttccaa cttgatacat actactctcc ttttcttttt cgattatatc ataaaagagg
    11521 cgagccaggc tcaacctctt tcacttaatt tgtacgaact ggtgtaatga gctgcatgaa
    11581 gtcttcgtca gtatctgctg gcacaagagt aaatggacga acagctgaga taaagctaat
    11641 agtcaccttt tcgctattta aagctttaag agaatcaatc aagtaagttg ggttgaaact
    11701 aatggtcaaa tcttcaccag taacctgatc agtatcgatt tcttcgttta ctttaccaac
    11761 ttctggagag tgaacatggg cgctaacaac cccatcctta atttcaagtt tcacagtacc
    11821 attttgagtc gcacttgata aaagacgggc acgctccatt gactggcgta agtttaccac
    11881 attaaaagta atagtagtgt taaagtctgt tggaatcaag cgatctgtat caggatagtt
    11941 tccttctagg agacgagtat agaagctaat attttcgctt ctaaagagga tttggttatt
    12001 ggcaaagaaa atctctacag tttcgatatc atctgtaaat accgctgaaa attcgcgtag
    12061 agaacggcta ggaattacga catcaaaatc atcactattt ttttcaagag tcaatttttt
    12121 ctggcttagg cgatgagagt ctgttgcaac tgtttttaac tctttgtgtt gactcaatac
    12181 gaagtggaca cctgttaaaa tcggacgact ctcttgtgta cttgcagcaa aggctgtttc
    12241 attaataatt ttcttgagta attttgtttc aagtattaaa ggagtgcttg ctgaaatttc
    12301 ttggattcgt ggatattgtt cgctatcttt tccttttagg gtaatttctg atttgccact
    12361 ggttaaaaca atttgatttt gttcaatttc tttaaaatca agagttacat caggtaaact
    12421 agatactaca ttgataaaga aagaagcttc aagaaggatc gaacctaaag aagtaattaa
    12481 caaaccagca tcttcatttt tttgagaaat aaaattttca attgaaattt gaccatttga
    12541 accaattaaa gtaatacctt cattggtcac gtcaattttt actgttgata aaataggaat
    12601 ggcattttta gaactaatag ctctcttagt agtatttaat gcttgtagaa ataaattttt
    12661 attaattgaa aaatgaatca tggattctcc tttatttatt tttagtatta gaaagataat
    12721 agtaataata gagtctgtga aatctgtgga aaacagcctt taaacaagtc atatcaagtt
    12781 ttttagcttg tttaaaaaat gtggataaaa aagataaaaa actaaaagtt atccacaaat
    12841 tatttgattt tctttttgat tgattcaatt tctaaacgta aattatcgtc ttgatcaatc
    12901 aaagatttta ttttggcatg agcatgaatg actgtggtat gatcttttcc cccaaattcc
    12961 ttcccaattt ttggaagact attatctgtt agttctctag ataaatacat ggatacttga
    13021 cgggccaaaa caatattttg aaggcgtcta cttcccttca tttctttgat actaacacca
    13081 taaaagttac caacttcagt ttggatttta tcaattggga tgacgagcat ttggctaaca
    13141 tcttgtttgc gggctctaat ggcttctgca gcaatatcaa tagtgatatc cttgattttt
    13201 tttactctgg caattaaagt gatgtcgttg atggctccct caagatctcg aacatttgaa
    13261 tcaaattgcc cagctaggta ttctagagta tcactttgga aattgtagcc taaatgttcc
    13321 gttttacttt gtaaaatggc aatacgtgtt tcaaagtcag ggggtgtgat agtttgtgtc
    13381 aatccccaac taaaacgcgt gacaagcctc tcctcgagcc cttctagatg ttttggacta
    13441 cgatcactcg ttaggacaat ctgtttttgc ttgtcatgaa gggcgttaaa ggtattgaaa
    13501 aattcttcct gagttgcgac tttttttccg ctgagtgact ggatatcatc gattaacaaa
    13561 agatcaagac tacgataggt ctttttaaac ttttccattt ccccaagtct taggtgatca
    13621 agaaagtcat taataaagct ttcggcaggg atatatttaa cacgcgcatt aggaatattt
    13681 tttagaattt catttccaat agcgtttaat aagtgagtct taccaaggcc tggtcctcca
    13741 tagataaaaa gagggttata ggtcagagcc aaatcttcag agacagctaa agcggctgat
    13801 acagcccaaa catttccatc cccttgaata aagttatcaa aggtatactt ttcttttaat
    13861 cccgtatctg aataaggaat agatactaac tttggactat agtcataaag agttaaattt
    13921 gtagcttctt caacttgtga gctagtcgta tcttgaggtt tggtgaaaat atagtgggga
    13981 gttatttcag cgtcataaat ttcaaaacca gctactacaa taatatcttt tagttgtttt
    14041 tcccagacca tttccatttc agagcgaggt agaaatatag tggcaacatt ttcctctacc
    14101 ttgataagtt cagcttgaat agcatagaaa tcatacatgg atcgagtcag tctttcttgt
    14161 gcaaattcta atatacgatt ccaaaattgt ttttctttca atcctttctc ctcctttact
    14221 atttaaaagt ttaatactag agatatttta ccatagatag caagaatttt ccacaagctg
    14281 tgaaaaataa tccacaatgt taccaaactt tatccacagg ttggggataa aagaagaaat
    14341 tattgattta ttaagctttt tatcgaaaaa catagtggat aaccttgtga gataaaaaaa
    14401 taaaagaaca gccttatttc aggctgttga taattctact atattcttct tgatttgaaa
    14461 aagaaataat gatttttcca ctgtcttttt tagatagttt aatttctaca tctaatccga
    14521 gtagttttct taactgtttt tcttcatttt gtatgaaatg attagttttt tgctgtttct
    14581 tttgtttttt ctctgtcaga agagcttcta atttccttac agaaatatct tcttctataa
    14641 tccgttgaaa gaaatagtct tgttgttcct tatttaaccc aactagggaa cgcgcatggg
    14701 cttgtgatag tttgccattt tctacttctg aaagaatctg ttctggcaag gaaagtaaac
    14761 gaatggagtt gctgatatat ggacgagact tgcccatctt atctgcaatt tcagcatggg
    14821 tgaatccttt ctctacgaga gattcatagg cgcgtgcttc ttctattggg tttaaatttt
    14881 ctctctgtaa attttcaata atggactgga ccatcatctc ttggtctgaa atctgtttaa
    14941 caacagctgg gatagaccgt agaccagcta aaagtgaagc ccgatagcgt ctctctcctg
    15001 caaggatttc ataaccaata acaggagatt gacgaacaat aatcggttga atgaccccat
    15061 tttctttgat agactgtgct agttcatcta gtttttctct atcaaattct tttcggggtt
    15121 gatagggatt tttttgtata tctgtgatag aaatcatttc aaatttttcc atgattctac
    15181 actaacacat cttttctctt atgtaaagct ttctttacat agatgtcaat taagattcta
    15241 aatcacctga actcttgtta agtttgatag aggtagtttc ttctttcccg ttacgatagt
    15301 aggttatctt aatggtgtct ccgatagaat ggttgtaaag agcactttgt aagtctgttg
    15361 atgaagcaat ctctttgtca tctacttttg taattacatc gtatttttca aggtgaccat
    15421 tggcaggcat attactttgt accgaacgaa caattacacc agatgtaaca ttacttggaa
    15481 tattgagtct tctgatgtcg cttgtactca cattagataa attaaccatc tggattccca
    15541 aagctggacg cgtcactttt ccgttttttt ctaactgttc aataatattg atagcatcat
    15601 ttgcaggaat tgcgaaacca agaccttcta cagatgttcc tccatttgta gcaattttac
    15661 ttgaggtaat tccgataacc tgcccttgaa tattgatcag tgggccgcca gagttacctg
    15721 ggttaatagc agtatcagtt tggatggctt ttgtagaaat agcttgtcca tcttccgatt
    15781 ttaaggatac atttctattg agactggata cgataccttg agtgacagta tttgcatatt
    15841 cagaacctaa cgggctaccg atggcaatag cagtttctcc tacagttaac ttactagaat
    15901 caccaaactc agctactgtt gtcacttttt ctgaagagat tttgacgaca gcaatatcag
    15961 agaaagtgtc agctccgaca atttctccag gtactttagt cccatctgac aatcgaatat
    16021 ctactttgct ggcgccattt ataacgtgat tgttggtgac gatgtaagct tctttatcat
    16081 tctttttata aataactcca gatccttcac tagagattcg ctgagaatct gtgtcagtat
    16141 catcattgcc aaatacgcta ttttgtctgt ttgccgaata agtaataaca gaaacaacag
    16201 catcttttac tttgttaaca gcctgtgttg ttgaattttc gttcttatag gcagtttgtg
    16261 taatagtact attgttgtta gagttgttta cactactttt ttgagttagt tgagttattg
    16321 aaaaactacc caaggctcca ctaaaaaagc taatgacgat aacgactaat aattgaaacc
    16381 attttttgta aaatgttttt agatgtttca tatttgcctc catatgtttg aattactgaa
    16441 agtataaact gactagctta attataactt aaacacaaaa gttttacaca aactgtggat
    16501 aactcttttg aaattgtgat tttcttaatt gaaatctatt ttttattttg tgaataagat
    16561 gtgaaaaaat agagaatatg ttagaataga gtcatgaaaa ttaaagttgt aacagttggg
    16621 aaactgaaag aaaagtattt aaaagatggt atcgcagagt attcaaaacg aatttctaga
    16681 tttgctaagt ttgaaatgat tgagttatca gatgaaaaaa caccagataa ggccagtgaa
    16741 tcagaaaatc aaaagatttt agaaatagaa ggtcagagaa ttttatcaaa aattgctgac
    16801 cgtgatttcg ttattgtgtt agccattgaa gggaaaactt tcttctcaga agaatttagt
    16861 aagcagttag aagaaacttc tataaaagga ttttctactc ttacttttat tattggggga
    16921 agtttaggat tatcatcatc tgtaaaaaat agagccaatc tttctgtcag ttttggtcgc
    16981 ctaaccttac cccatcagtt aatgagacta gttcttgttg aacaaatcta tcgcgctttt
    17041 acgattcagc agggattccc ctaccataaa tagaaaattg acttttaatt gaatttttgg
    17101 tagaataatt gtattaggtc tcatagctca gctggataga gcattcgcct tctaagcgaa
    17161 cggtcgcagg ttcgaatcct gctgggatca atataatagc aaagctggga attttcccgg
    17221 cttttttctt aaaaaagtac actttgggag aaaaaaatga cagttgagag aattttatct
    17281 aaaacgaaat tccattttgt ataatggttt ttgtaagtta gcttacaaga aaaaacattt
    17341 taggagattt tattatgaaa aacacagtta aattggaaca gtttgtagct ttgaaggaaa
    17401 aagacttaca aaagattaaa ggtggggaga tgaggatttc aagaatcatc cttgattttc
    17461 ttttcctacg aaaaaagtaa tgaaataagg agaaagagta atggatttac ttggatttgg
    17521 gacagttatt gttcattttt taattattag tcacagttac cgtttaattt gtaaaggtcg
    17581 aataaataga aaagaattat acgtttttgg tgcttataca ttactaactg aaatagtact
    17641 tgaattttcc ttttatcttc tatatttaga taaaataggg attgaaagat ttttatttcc
    17701 tttgggctta tattcctatt ttcgatggat gaaacagtat gagagggata gaggactatt
    17761 cctaagttta ctactatctc ttttatatga gagcactcat aactttctgt ccgtaatttt
    17821 ctcctctata acaggagata attttgtttt acaatatcat ttcccattct ttttcgttgt
    17881 gacggtgtta acctattttg ttacattaaa aatcatttac tatttccatt tggaactagc
    17941 ctattttgac aaggactacc tttatccttt cttgaaaaaa gtattttttg ctttactatt
    18001 gctacatatt gtatctttcg tttcagatat ggtaagtacg attaaacatt tgaatagttt
    18061 tggaagtatt ttgtcatcta ttgtctttat ctctctcctt ttgaccttct ttgcaatgaa
    18121 ttctcataaa gttcaaatgg agaaagagat tgctttgaag cagaagaaat ttgaacagaa
    18181 acatttacag aattacacag atgaaattgt tggtctgtat aatgaaatcc gtggttttcg
    18241 acatgattat gctggaatgc ttgtcagcat gcagatggca attgacagtg gtaatttaca
    18301 gaatttacaa tgaagtttta gtcaaagcaa atcataaatt gcgttcagat aagtacactt
    18361 actttgattt gaacaacata gaagactcag ctttacgaag tttggttgct cagtcaattg
    18421 tctatgctcg aaataatggt gtagagttta cactggaagt aaaagatacg attaccaagc
    18481 ttccaattga actattggat ttggttcgta tcatgagcgt tttattgaat aatgctgtcg
    18541 aaggatcggc tgatagctat aaaaagcaga tggaagtagc agttattaag atggaaactg
    18601 aaacagttat tgtgattcag aattcatgta aaatgacgat gactccttca ggagatctat
    18661 ttgccttagg attctccact aagggaagaa atcgcggagt cggattaaat aatgtgaaag
    18721 aactactaga taagtacaac aatattattt tagaaacaga gatggaaggc agtacattta
    18781 gacaaatcat tagatttaag agggaatttg aatgaaagtt ttaattttag aagatgttat
    18841 tgaacatcaa gtgagactag agagaatatt ggatgaaatt tcgaaagaat cgaatattcc
    18901 aatatcatac aagacaacgg gaaaagtccg tgaatttgaa gaatacattg aaaatgatga
    18961 agtaaatcag ctttatttcc tagatatcga tattcatgga attgagaaaa agggatttga
    19021 agtggctcag ctcattcgtc attacaatcc ttacgctatt atcgtcttta tcactagtcg
    19081 atcagagttt gcgactctaa cctataaata ccaggtatca gccctagatt ttgttgataa
    19141 ggatatcaat gatgagatgt ttaagaagag aattgagcaa aatatcttct acacgaagag
    19201 tatgttactt gaaaatgaag atgttgtaga ttatttcgac tacaattaca agggaaatga
    19261 tttaaaaatt ccttaccatg atattttgta tattgaaaca acaggggtat ctcataaatt
    19321 gcgcattatt ggtaagaatt ttgcaaaaga gttttatggt accatgacag atattcagga
    19381 aaaggacaaa catactcagc gattttattc tcctcacaag tcatttttgg taaatatagg
    19441 taatatcaga gaaattgatc gaaaaaactt agaaattgtt ttctatgaag accatcgttg
    19501 tcctatttca agattaaaaa ttagaaaatt aaaagatatt ttagagaaaa aatctcaaaa
    19561 gtgattgaca attagcaaga aattgatata atggttatat ctgctacaga tagaaggtcc
    19621 gttggtcaag gggttaagac accgcctttt cacggcggta acacgggttc gaatcccgta
    19681 cggactattt tatccgccat agctcagttg gtagtagcgc atgactgtta atcatgatgt
    19741 cgtaggttcg agtcctactg gcggagtata gataaaaggg aacacagctg tgttcctctt
    19801 tttgtatcaa tttgtatcac caagcatttt cataaggaag tctgttattt cttgagaact
    19861 ttcttttttt ccatgtgcaa tccaagtttg gcagacacca aaaagtgcat gagttagata
    19921 gatgctacta tattctaatt cagtggtatt tagattcagt tgcataaatc gcttttgtaa
    19981 atctgtacta agcatgatat gaagtttatt tcgtaagaaa ttttggattt ctttagtccc
    20041 attttcagaa agaagggcag ccagaagtgg ttctgactct agatattcaa aaacttctaa
    20101 aatagcgtct cttttgtgat gagcatgttt ttgaaaaata tattcaaatg tatggaatag
    20161 cttgctttga tagtgctcaa tcatatcata cttatcctta tagtgagtat agaagctgga
    20221 acgactaatt ccggcttttt ctactaattt gacagtagaa attttatcaa atggctgttc
    20281 catcagtaat tgtaccatag cattttcaat agttcgcttt gtttttaagc gtttgttact
    20341 ttcttgcata tttcctcctt gtaaacaaat tagactatat gtctaaaaat agatttttta
    20401 tcttgtaatt tagatttttt aatgtataat ctattatatc aaaattttag acaatatgtt
    20461 taaaaaagga gaaactaagt ttaaagaatg gaaagcaatt taaaaaaaac caacctttat
    20521 tattgtcatg atcgggattt ctcttattcc agatctgtac aatatcatat ttttgtcatc
    20581 aatgtgggat ccatatgggc aattgtctga cttacctgtg gcagttgtaa ataatgataa
    20641 agaggcttcc tataatggta atactatggc aataggaaaa gacatggtgt ccaatttaaa
    20701 agaaaataaa accttggatt ttcattttgt agatgaagag gaaggaaaga agggattgga
    20761 agatggcgat tactatatgg tagtgacttt accaagtgat ttatctgaaa aaacaactac
    20821 attatccaat attcaatcga cagcagctta tcaatcattg acaagtgagc aacaaactga
    20881 gataagtgat tctgtatctc aaaattcaac tgatagtatt caatcggctc agtcaattgt
    20941 agctttagta caagatttac agggaagttt agaaaactta caaaatcaat cttctaatct
    21001 ttcgacttta aaaaatcaat ctaatcaagt atcacctatt acttctactt ctttgatagg
    21061 attgtcaagt ggattaacag agatacaagg agatgttact agcaaattag ttcctgccag
    21121 tcagtcgatt gcatcaggtg taaacgcata tactacaggt gttgataaag tttctcaggg
    21181 cgcaagtcaa ctaagtgaaa aaaatgccac cttgacaggt agtttggata aactagtttc
    21241 aggctcaaac accttgacac aaaaatcttc tagattgaca gcaggagttg gttaattaca
    21301 atcaggatct gggcaattag cagacaaatc cagtcagtta ctttcaggtg cttctccatt
    21361 agagaataga gctaataaat tggcagatgg atctgggaaa ctagcagaag gtggaacaaa
    21421 gttaacttct ggattggaag atttacagac aggacttgct tctttaggac aaggactagg
    21481 taatgctagt gatcaactca aatcagtatc aacagaatct aaaaatgcag agattttgtc
    21541 aaatccactc aatctttcaa aaacagacaa tgatcaagtt cctgtaaatg gaatcgcaat
    21601 agctccttat atgatatcag ttgctctttt tgttgcagca atatcaacaa atatgatatt
    21661 tgcgaaattg ccttcaggac gtcatccaga gagccgttgg gcttggttga aatctcgagc
    21721 tgaaataaat ggtattatag ctgttttggc aggaattttg gtatatggag gagttcagct
    21781 tattggttta actgctaatc atgagatgag aatatttatt ctcatcatcc taacaagttt
    21841 agtattcatg tctatggtga ccactttaac aacgtggaat agccgtatag gagctttttt
    21901 cttacttatt ttgcttttac tacagttagc atcaagtgca ggtacttatc cacttgcttt
    21961 gacaaatgat ttctttagat ctattaatcc ctggttacca atgagctatt cagtttcggg
    22021 attacgacaa acaatctcta tcaacaagtc attttcctag ctgtcatact agctctattt
    22081 actggtttag gtatgctagc ctatcaacat aagaaaatgg aagaagatta aaaaaatcga
    22141 ccgattaatg aactgcaccc caaaagttag acagaaaaaa tctaactttt gaggt
//
LOCUS       DAVBCN010000029        21254 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVBCN010000029 DAVBCN010000000
VERSION     DAVBCN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21254
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.029"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(166..780)
                     /locus_tag="VQ497_001036"
     CDS             complement(166..780)
                     /locus_tag="VQ497_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HET2517356.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(1595..1864)
                     /gene="rpsO"
                     /locus_tag="VQ497_001037"
     CDS             complement(1595..1864)
                     /gene="rpsO"
                     /locus_tag="VQ497_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET2517357.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(2090..2635)
                     /locus_tag="VQ497_001038"
     CDS             complement(2090..2635)
                     /locus_tag="VQ497_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET2517358.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGSGS
                     LYIDETKTTRLLDTPDFPEEFKSLAKAQADELDLLRTKNNLNWAFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKRNKPD"
     gene            2874..3074
                     /locus_tag="VQ497_001039"
     CDS             2874..3074
                     /locus_tag="VQ497_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517359.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKGLYINNLL
                     FFTPMVILIVSLLYSYRFRFSL"
     gene            3505..3645
                     /locus_tag="VQ497_001040"
     CDS             3505..3645
                     /locus_tag="VQ497_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HET2517360.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQ"
     gene            3652..3828
                     /locus_tag="VQ497_001041"
                     /pseudo
     CDS             3652..3828
                     /locus_tag="VQ497_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
     gene            3886..5829
                     /gene="thrS"
                     /locus_tag="VQ497_001042"
     CDS             3886..5829
                     /gene="thrS"
                     /locus_tag="VQ497_001042"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET2517361.1"
                     /translation="MINITFPDGAIREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHGDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(5878..6852)
                     /locus_tag="VQ497_001043"
     CDS             complement(5878..6852)
                     /locus_tag="VQ497_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HET2517362.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVEMQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(6845..7522)
                     /locus_tag="VQ497_001044"
     CDS             complement(6845..7522)
                     /locus_tag="VQ497_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2517363.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            7678..8751
                     /locus_tag="VQ497_001045"
     CDS             7678..8751
                     /locus_tag="VQ497_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HET2517364.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDKLAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHKIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            complement(8842..9144)
                     /locus_tag="VQ497_001046"
     CDS             complement(8842..9144)
                     /locus_tag="VQ497_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517365.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(9212..9649)
                     /locus_tag="VQ497_001047"
     CDS             complement(9212..9649)
                     /locus_tag="VQ497_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET2517366.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKKSKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELTSVARGTGGTDIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(9725..10567)
                     /locus_tag="VQ497_001048"
     CDS             complement(9725..10567)
                     /locus_tag="VQ497_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET2517367.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            10679..11329
                     /locus_tag="VQ497_001049"
     CDS             10679..11329
                     /locus_tag="VQ497_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET2517368.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRMHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            11539..12173
                     /locus_tag="VQ497_001050"
                     /pseudo
     CDS             11539..12173
                     /locus_tag="VQ497_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
     gene            12304..12741
                     /locus_tag="VQ497_001051"
     CDS             12304..12741
                     /locus_tag="VQ497_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517369.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISNTYTILSISSFLV"
     gene            complement(12836..13135)
                     /locus_tag="VQ497_001052"
     CDS             complement(12836..13135)
                     /locus_tag="VQ497_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517370.1"
                     /translation="MNRNVLNFLRTESAERVSLYIDKANRLEGDVTLLAPSSQDLEDI
                     KNAMFSNPNLELKVARLDVMKKIAYASTRNHYLTGATIFGDISKGTYNCDPKSYV"
     gene            complement(13543..13905)
                     /locus_tag="VQ497_001053"
     CDS             complement(13543..13905)
                     /locus_tag="VQ497_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517371.1"
                     /translation="MRLASESRQILDKLKDDVYNKLGYEVSYSSIVSQAVREYVPKKE
                     RIDWIKLKETAIPFSSLKQSNNWEYQTSLMLEKDVLILLSELQNFFLDVFQAKRIHRA
                     FCIRLCLRAQFLLSNNDS"
     gene            14264..14678
                     /locus_tag="VQ497_001054"
                     /pseudo
     CDS             14264..14678
                     /locus_tag="VQ497_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14717..15160)
                     /locus_tag="VQ497_001055"
     CDS             complement(14717..15160)
                     /locus_tag="VQ497_001055"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HET2517372.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(15190..17412)
                     /locus_tag="VQ497_001056"
     CDS             complement(15190..17412)
                     /locus_tag="VQ497_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET2517373.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRMIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            17823..18065
                     /locus_tag="VQ497_001057"
     CDS             17823..18065
                     /locus_tag="VQ497_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517374.1"
                     /translation="MTRFEEIFEEYKNPQDTFFYPLVYKENTYEKTAISIFALLMLGV
                     CCLFLFSQQSYKKLVQYYANDQNLPSRITYSEYSDK"
     gene            18405..19034
                     /locus_tag="VQ497_001058"
     CDS             18405..19034
                     /locus_tag="VQ497_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET2517375.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(19157..21049)
                     /gene="pepO"
                     /locus_tag="VQ497_001059"
     CDS             complement(19157..21049)
                     /gene="pepO"
                     /locus_tag="VQ497_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HET2517376.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         6288 a         4745 c         3828 g         6393 t
ORIGIN      
        1 ttcttatttc attttactat atttataaga atctcctaat caaatcaaaa gccactctac
       61 aatagagtaa ctttaagatt gtccatttat actcaatgaa aaagccctct gaaaatcaga
      121 gagctgattt gagctcggac taaaatccta gtgaaacaaa aaaatctaca cgatcagaaa
      181 agtctctatc gtgccatttt catacatgat gtatagtcca agtagaatga atactaaggg
      241 cacaatgatt cgctcgtatt tttcaattgt ctcgaatatt aacggaatag aagataacac
      301 ccgactaatc tcgcaaaaga taattatgcc gattacaaac acaagcaagg ccacgagggt
      361 ctgtgaccaa tctaacgaag caaaataagg tatatagata cctaaattat ctccgccaga
      421 cgcaattgtc agcaatgtaa ctgtccaaaa cagttgattt gccttgcttt gttctaatct
      481 ttcaataatt tcttcctctt cttcttccgc atcttctcca acaattgcaa agcgaatccc
      541 taaatagata gggattaaac caagcaatcc aaccatccat tcttcaggca cgaaattaac
      601 gacataagca gcaactaaac tcgcccctac aagtaagcct gtgcctagat attgccccgc
      661 ataaatatgc catttctgtt tattctgtga tagctgtgca aataaaataa ttaaaataat
      721 taaataatcg atactggtgg aaatataaac accaatagca gatatgattg tctgtcccat
      781 aaaaaaaact cctgtattag tcagtaataa atcagcagat tttaggagag cacagacaca
      841 ttaatttagc tatcgctagt gggtaatatc gaacgtagga aagcatttta ctcctcctca
      901 aaacgtagtt acaaagtaat ttctaactgg aattcagtga ctacttccat gtaaagtata
      961 acatactata ctttactttg taaagagtgg gctcttctag ggggcttgca tacaactact
     1021 cggcttgaaa agccgagtaa ctgcgcacct ttatggtgta attagctgat accgtttgag
     1081 gtttttgtag tctccaaaat tgttaccgat aaaacctcaa taaaaaagcc ctctgaagtc
     1141 agagagctga tttgagtccg ggctaaaatc ctagtgaaaa agatgaaact ccttgtgctc
     1201 atcgaacact gtgtcatttc cctattttca tacggatttt tgacgccctt agcatcatga
     1261 ttcttgctag ataaatcgtt tatagactag gcgacgaggc gaagatcgta cgaaactttc
     1321 agtgaaacaa aaaaaatctc cccgaaggga gaaaagctgg tttattttac cttacagtgc
     1381 taggaaccgt aaccaaagat tatacttgag tatatcgagg taaggtgaca acacaaaaaa
     1441 gctacccaat ttagagtagc ttcattatca agatatgctc aattgaacac ggcctaagca
     1501 cttggcaaaa aagataaaat ctcctagaaa ctgaagtttc ttcgtaaatt ttcctatttt
     1561 tactttgtgc ttttgacagc ctttgtatct tgaattagcg acgaagtcct agagagttga
     1621 tcaactcacg gtaacggtta acgtcgtttt tacgcaagta tgcaagcaag ttacgacggc
     1681 gaccgatttt tttcatcaat ccacggtaag tagcgtggtc ttttttgtgt tgtttgatgt
     1741 gttcattaag gtggttgatt tcccaagtaa ggacagcaac ttgaacctct actgaacctg
     1801 tatcaccttc gtgacgtgca tattgtgcga tgatttcatt ttttttctct tttgagattg
     1861 ccatgatgtt tctccttttt atttggcttc atccgagtga caggttggca tgcctgtaac
     1921 caagaagaag ttatttgtct ttacgacaat ccctattcta ccaagattta aattttctgt
     1981 caaacattaa tactctcata gttattattt ttcaagtaaa gagatacgtt cttttatatg
     2041 atgacctttt tcaagttcat ctaccaatcc aatagcataa tctgcataac taatctggct
     2101 tattcctttt ttcattagta gtaaaaattt ctcctgctag aatgtagttt cctgtttttt
     2161 caccatcggg aataaaatct acagcaggac tgacaaaagc ccagttgaga ttattttttg
     2221 tacgaagaag atccaattca tccgcttgag cttttgctag tgacttaaat tcttcaggaa
     2281 aatcaggcgt atctaaaagt cgtgtcgttt ttgtttcatc tatataaagg cttcctgatc
     2341 cccctacaac tagaaaacgt gtctgtgttc cagcaagaag ctgattaaat agttcgattg
     2401 atttgctgtg gagcggtagc gtatctggtg tataagcacc aaacgctgaa ataacagcat
     2461 caaatccagt aagatcatct tttgtcaact caaataaatc ttttttaata atagactcag
     2521 cttgactttt gttttcagaa cgaacaatag ccgttacttc atgtcctcgt ttgactgctt
     2581 cttcaacaat tgccttcccc gcttgtccat ttgctgcaat aactgctagt ttcatttttt
     2641 atacccctct tgttgtaatt attttagtta caacaattgt gacactctta ataatcaatg
     2701 tcaatagtct tgcttaatta ttatcaaaat atttctacca agaaaactaa ccatgattct
     2761 agtgaaaaga aaaatcttct ttgtcaacaa atttactttc ttgttttaaa catactataa
     2821 taatcatagc aagagatcta agttgtctgt ttttttaaaa cgaggtgatt atcatgcgta
     2881 gattctattc ccatctcccc tactatctgg tcatattatt cttttattgg ccactttatg
     2941 agttgttctt gctagttgtt tctgaccccc ttacactcaa gggactctat ataaacaatc
     3001 ttctcttctt tacacctatg gtaatcttga ttgtatcgtt actctatagc taccgtttcc
     3061 gtttctcact ttgatggtta gttggtaacg gactgctctt ttactttact atcataacct
     3121 ttggtgtgtt tatactaatt tacttgctaa tctatgaaac agttgctctg gtgggcatgg
     3181 attctggtat tagcatcaag catattctac aaaaaatgaa aaacaaaaaa ctttcacaaa
     3241 atccttgaaa aatctcacaa tcatgctata ataatccata gagacaagtc acttagtccc
     3301 tttctactag agagtgcgtg gttgctggaa acgcatagga agtctaaact gatactactc
     3361 ttgagttttt tatgaaaaca taaaacggtg gccacgttag agccgatcag aggtgtccct
     3421 ctcttttgag gtacataaat gaaggtggaa ccacgttgcg acgtcctttc gaggatgtcg
     3481 cattttttta ttaggatact aattatggag ttgcaagaat tagtggagcg cagttgggca
     3541 atccgacaag cttatcacga actggaagtt aagcatcatg atttcaagtg gacggtagaa
     3601 gaagacctct tggctttatc taatgatatt ggaaatttcc aatgactggt gatgacaaag
     3661 caaggacgct actatgatga aacaccctac acactggaac aaaaactttc agaaaatatc
     3721 tggtgactat tagaactttc tcaacgtttg gatatagata ttctgacgga aatggaaaac
     3781 ttcctctctg ataaagaaaa gcaattgaac gttagaactt ggaagtagtc tgctgataaa
     3841 aaatcaatgc ttagaaacta tgaaataata aaaaaggaga acatcatgat taacattact
     3901 ttcccagatg gcgctattcg tgaattcgaa tctggcgtaa caacttttga aattgcccaa
     3961 tctatcagca attccctagc taaaaaagcc ttggctggta aattcaacgg caaactcatc
     4021 gacactactc gcgctatcac tgaagatgga agcatcgaaa ttgtgacacc tgatcacgga
     4081 gatgcccttc caatcttgcg tcactcagca gctcacttgt tcgcccaagc agctcgtcgt
     4141 cttttcccag acattcactt gggagttggt ccagccatcg aagatggttt ctactacgat
     4201 actgacaaca cagctggtca aatctctaac gaagaccttc ctcgtatcga agaagaaatg
     4261 caaaaaatcg tcaaagaaaa cttcccatct attcgtgaag aagtgactaa agacgaggca
     4321 cgtgaaatct tcaaaaatga cccttacaag ttggaattga ttgaagaaca ctcagaagac
     4381 gaaggcggtt tgactatcta tcgtcagggt gaatatgtag acctctgccg tggacctcac
     4441 gttccatcaa caggtcgtat ccaaatcttc caccttctcc atgtagctgg tgcgtactgg
     4501 cgtggaaaca gcgacaacgc tatgatgcaa cgtatctacg gtacagcttg gtttgacaag
     4561 aaagacttga aaaactacct tcaaatgcgt gaagaagcta aggaacgtga ccaccgtaaa
     4621 cttggtaaag agcttgacct ctttatgatt tcacaagaag tgggacaagg tttgccattc
     4681 tggttgccaa atggtgcgac tatccgtcgt gaattggaac gctacatcgt aaacaaagag
     4741 ttggcttctg gctaccaaca cgtctacact ccaccacttg cttctgttga gctttacaag
     4801 acttctggtc actgggatca ttaccaagaa gacatgttcc caaccatgga catgggtgac
     4861 ggggaagaat ttgtccttcg tccaatgaac tgtccgcacc acatccaagt tttcaaacac
     4921 catgttcact cttaccgtga attgccaatc cgtatcgctg aaatcggtat gatgcaccgt
     4981 tacgaaaaat ctggtgccct cactggcctt caacgtgtac gtgaaatgtc actcaacgac
     5041 ggtcacctat tcgttactcc agaacaaatc caagaagaat tccaacgtgc ccttcagttg
     5101 attatcgatg tttatgaaga cttcaacttg actgactacc gcttccgcct ctctcttcgt
     5161 gaccctcaag atactcataa gtactttgat aacgatgaga tgtgggaaaa tgcccaaacc
     5221 atgcttcgtg cagctcttga tgaaatgggc gtggactact ttgaagccga aggtgaagca
     5281 gccttctacg gaccaaaatt ggatatccag attaaaactg cccttggaaa agaagaaacc
     5341 ctttctacta tccaacttga tttcttgttg ccagaacgct tcgacctcaa atacatcgga
     5401 gctgatggcg aagatcaccg tccagtcatg atccaccgtg gggttatctc aactatggaa
     5461 cgcttcacag ctatcttgat tgagaactac aagggggcct tcccaacatg gctggcacca
     5521 caccaagtaa ccctcatccc agtatctaac gaaaaacacg tggactacgc ttgggaagta
     5581 gccaagaaac tccgtgaccg cggtgtccgt gcagacgtag atgagcgcaa tgaaaaaatg
     5641 cagttcaaga tccgtgcttc acaaaccagc aagattcctt accaattaat tgttggagac
     5701 aaagaaatgg aagacgaaac agtcaacgtt cgtcgctacg gccaaaaaga aacacaaact
     5761 gtctcagttg ataattttgt tcaagctatc ctagctgata tcgccaacaa atcacgcgtt
     5821 gagaaataag agtctagcat aaaagcctcc aatctggagg ctttttctca tctattttta
     5881 ctcaaggact aagttcactt gagcaaactg aatccgcact gtcgttcctt ttccgacctc
     5941 agactcgata cgaatctggt gccccagttc ttcagaaatt ttcttagata gataaaggcc
     6001 aagtccagag gactgctggg tcaaacggcc attgtatcct gaaaagccac gttcaaatac
     6061 tcggaggaca tcactgtttt ttatcccgat tcccgtatct ttgatacaaa gctcttggtc
     6121 atccatataa atctccagac caccttcctt ggtgtacttg agactgtttg agatgatttg
     6181 ctcaataacc actagcagcc actttttatc cgtcacgatt tctttatcaa ggtcatgtag
     6241 attgacattt aagccttttt gaataaagaa aagagcatat ttacgaatta tttccttgac
     6301 caagtcctca atttgaacct gctttaagag caaatcatca tggaaacttt ctaaacgcag
     6361 gtactgtaaa actaggttgg tataagagtc gattttgaaa atttcctgtt ctagctgctg
     6421 cttcagttgg cggtcgacca cttctgcaac taagagttga ctggctgcaa tgggggtctt
     6481 tatctgatgg acccacaagg tatagtaatc cagcaaatcc gtcagttttc tttctgcttt
     6541 tgacctctgc tgatagagtt ccatctcacg cgcttctaat ttttctgcta aagctatttc
     6601 caaaggagac ttggcttccc tctctccata gagaagttcc tggcgataga cctgcatttc
     6661 caccaatatg tcccaagtga aaaataagat ggttacaaag caacacaaga agaaaaagta
     6721 gaggaagtaa attcctagac tggcaaataa aaactgaaag agtaagacaa gaaatgccaa
     6781 agaaagcaga tagataaaaa gacgactacg ggagcgcaga taggctagaa aaaattgttt
     6841 ccaatcaagc atgcttcaat ccgtacccta ttcctttctt ggtctcgata aatcctacca
     6901 atccctgctc ctccaacttt ttacgcaaac gagccacatt gacagagagg gtattatcat
     6961 caatgaaaaa gtcactgttc caaagttccc gcatcaggtc gtcacgtgct acgatgttgc
     7021 ctgcatgctc aaataacacg cgtaaaatct ggaattcatt cttggtcaaa ttcaagactt
     7081 gcccttgata atgtaaatcc atggatttgg tattgaggat aacaccagca tattccagca
     7141 aactctcatc acgcccaaac tcataggaac gacgcaacaa gccctgaacc ttagctaaaa
     7201 gaacctgctg gtcaaaaggc ttggtcacaa agtcatccgc ccccatattg attgccatga
     7261 caatatccat agcctggtct ctcgaagaaa gaaacatgat aggtaccttg gaaatcttgc
     7321 ggatttcctg acaccagtga taaccattaa acaagggcaa accaatatcc atgaggacca
     7381 gatgaggttc cgactgaaca aatagactca aaacttccat aaagtcttct accaggacca
     7441 cttcaaatcc ccattcagag agcattttcc caatctgttg acgaatgacc tgatcatctt
     7501 ctattaataa aatcttgtgc atgcgcttct ccttttccat tattataaca gatttttcca
     7561 tgctagatgg tctgaaactg aatttgaaat agcctgtttt tagccagtgc aaacaggcta
     7621 tgctactagc taatttgagg gaaatttgct aagataaata aaaagaaagg agctcttatg
     7681 gccaatattt ttgactatct gaaagatgtc gcatatgatt cttattacga ccttcccttg
     7741 aatgagttag acattctaac cttaatagaa atcacctacc tctcctttga taatctggtc
     7801 tccacacttc ctcaacgtct tttagatcta gcacctcagg ttccaagaga gcccaccatg
     7861 cttactagca aaaatcgcct tcaattatta gataaattgg ctcaacacaa gcgcttcaaa
     7921 aattgcaaac tctcccattt tatcaacgac atcgaccctg aactgcaaaa gcaatttgcg
     7981 gctatgactt atcgtgtcag cctcgatacc tatctgattg tctttcgtgg gacagatgac
     8041 agtatcattg gctggaagga agatttccac ctgacctata tgaaggaaat tcctgctcaa
     8101 aagcacgccc ttcgctattt aaagaacttt tttgcccatc atcctaagca aaaggttatt
     8161 ctagctgggc attccaaggg aggaaatctc gctatctatg ctgctagcca aattgagcaa
     8221 agtttgcaaa atcagatcac agcagtttat acatttgatg cacctggtct ccatcaagaa
     8281 ttgacacaga ctgcgggtta tcaaaggata atggatagaa gcaagatatt cattccacaa
     8341 ggttccatta tcggtatgat gctggaaatt cctgctcaca aaatcatcgt tcagagtact
     8401 gccctgggtg gcatcgccca gcacgatacc tttagttggc agattgagga caagcacttc
     8461 gtccaactgg ataagaccaa cagtgatagc cagcaagtag acacaacctt taaagaatgg
     8521 gtggccacag tccctgacga agaacttcag ctctacttcg acctcttctt tggcactatt
     8581 cttgaggctg gtattagctc tatcaatgac ttggcttcct taaaggcgct tgaatacatt
     8641 catcatctct ttgtccaagc tcaatccctc actccagaag aaagagaaac cttgggtcgc
     8701 cttacccagt tattgattga tactcgttac caggcatgga aaaatagata atactcttga
     8761 aaattaaatg tatacaaaac aaaagaccta gaatacatac tttcatgtgc attctaagtc
     8821 tttttaaata gaatctaata gtcaataaaa atcaaagagc attgagagat aatggggctt
     8881 ggaacgtccc tctcgcttca acaaaatgac cccattatag attaaaaaga tgccacttag
     8941 aaaaagcaaa aaaggaagta agacaaaggc aaatacataa aaagctaact gaacattctc
     9001 gtatccattt ttataaaaaa ggtaggatag ataaaaataa cttgaaatga gggataataa
     9061 aaataatact ggattccaca aacttctatt atccttccaa aatgacacta taaaggctaa
     9121 tacaattcct ataacgagat acatttctta ctcctttaat agctacattt tatcataatt
     9181 atccaaagaa aaaagagggc atttatccct cttaatcctt catctgactc tctgcatcgg
     9241 ccacgacttt ttctagactg gtttgaccaa gttctgcctc catagtcaac tgaattctct
     9301 ccaatttttg atccaaaaca tcatgaatat gagctcctac agggcaattt ggattcggat
     9361 tgtcatggaa actgaagagt tgacctgtct taccaagaca ttcgaccgcc tgataaacat
     9421 ctaaaagact aatatcctta aggtccttga caatatctgt tccgcccgtt ccacgcgcta
     9481 ctgaagtcag ctctgccttc ttcaactggg acaagatctt tctgataatg acaggattga
     9541 ccccgacact agcagccaga aaatcactgg tcaccttgct tttcttcccc tcgagggcaa
     9601 tgattatcag catatgagtc gcaatggtaa atctacttgg aatttgcatc ctcttctcct
     9661 ttttacgagg ctaccctgcc tctactcttc tttttctatt attataccct ttttagttgt
     9721 aatgtcaatc gttaccactt ttcaaccagt cgtctaactc ccgatcgcaa ccctctttct
     9781 gagccaattc tctcaaaaat tcctgatgat gagtatggtg gatcccattg accagacttt
     9841 catagtaaac ctcaaaatag ggaagtctca ggtctttagc cagctgcaat tcagctgcca
     9901 catcgtaatc tacccgtcgg aagtccatat ctaccaggcc cttgtcatca aactccaaaa
     9961 tcatatactg ggcccgcaag tccttccgta gctgagcgtc caaaaagaaa ggttggccaa
    10021 tcgaacccgg attgacaatc aattgcccac cagtcccgta acgaagcaac tgctggtgaa
    10081 tgtgtccata aacagcaata tcacagggag gatgagtcac caagcggtca aattcctctt
    10141 gtttgccagt atgaatcaac tctcgccccc agttcttatc aggcagatga tggctaattc
    10201 ccactgtcaa atccccaaac tgacgatgaa tttgaagagg ttgattgtgg agcacttcaa
    10261 tttcttctag ggaaatttcc tctaaaacat actggcactg gcgcaagaga tagcgttgac
    10321 tagggcgagt actatccaat tccttacgga caccatgcca aagactgtct tcccagtttc
    10381 ccaaaactct agccgtaatc ggtagttgat ccaacaagtc caaaatcctt ctacgccctg
    10441 tccctggcat gagaatatct cccaaaagcc agtattcatc cactcctagc tgccgagcat
    10501 ctgccaaaac agcctccaag gcggtggtat ttccatgaat atctgaaaga agagctattt
    10561 tcgtcatatc catctcctcg ttttttctct tgcaataagt ataacataaa aagtcacagc
    10621 tagagaaatc tagctttttt tgatatacta gataaagata ttagacaaga ggaaacgaat
    10681 gaccccaaac aaagaagact atctaaaatg tatttatgaa attggcatag acctgcataa
    10741 gattaccaac aaggaaattg cggctcgcat gcaagtctct ccccctgccg taactgaaat
    10801 gatcaaacga atgaaaagtg aaaatctcat cctaaaggac aaggaatgtg gctatctact
    10861 gactgacctc ggtctcaaac tggtctctga gctctatcgt aagcaccgct tgattgaagt
    10921 ttttctagtt catcatttag actatacaag tgaccagatt cacgaggaag ctgaggtctt
    10981 ggaacacact gtctctgacc tgttcgtgga aagactagat aaactgctag gtttccctaa
    11041 aacctgcccc cacggaggaa ctattcctgc caagggagaa ctcctcgttg aaatcaataa
    11101 cctcccacta gctgatatca aggaagctgg cgcctaccgc ctgactcgga tgcacgatag
    11161 ttttgacatt ctccattatc tggacaagca ctcacttcac atcggtgacc agctccaagt
    11221 taagcagttt gatggcttca gcaatacctt cactatcctc agtaacgacg aggatttaca
    11281 agtgaatatg gacattgcaa aacaactcta tgtcgagaaa atcaactaat ttctcaagtc
    11341 ccctaccaac cctgaaagtt ttattttcag ggttttattt ctatcatttt attttacttt
    11401 catttggtgg tagaatattt gaaaaaaaat gggagaatat ttgaaaaaaa gaaaggtagt
    11461 ttaacctatg aaaaaatcac taaaaatttt tgctacatct aaatagtttg acctcttcgg
    11521 ggttgctttg gtcgttggat tgcgattgca tctggttacc tcaactcacg cctcgataaa
    11581 tttgtagact ggggaccatg gacagctctt gtaccctttg gattgatttc cgtaaccaac
    11641 gttgggattt ccatgttgtc cactcgtttc acggggaaat taagcaaatg ggaaaattac
    11701 tttggtattg ttaataccat tttgtccggt gctattgatt atatccttgg aaataaggcg
    11761 gccatcatta cctatcccgt caccttcctc atttatacct ttgctataaa gaaatggaaa
    11821 gcttcgcaag aaggcagacc caaccaaatg agccaaaaac aggtaaaatt ggcggccatc
    11881 atcatttcca tcatcgcctt cctctttgcc tttgtgacca actatatcgg atatgggggc
    11941 aagatgaatc tccttgccta cgtaacaact attgcctttg cactgtccct cattgccaat
    12001 gctttgaatg cattggcaat gagggacagt ggggcttttg gttgatttac aatttcgttc
    12061 agctgacaaa agctggtatt caaggaaact tcgccaatat cggtaaatac atcttttata
    12121 tcctcaatgc aatcggagct ttatttgtct ggaatgatga agaagttaga taatagaagg
    12181 aaactcaaat aaagaatact agccaactta agagaatctc ccctctgcta tttttatcat
    12241 tttagttgta actcccattc ttttagagta gaatgaagtt agataaaaga gggaggtctt
    12301 gtcatgaaaa aactctatag aatccatttt atagcaattg cagttattga tttgctacta
    12361 tttgcatttt ttatcactag actcgagaca tcttttgagt ggctcttgct ctctggttta
    12421 attttcttcc ttgctcaagg actcctgcta tttctcttgg tcgtccgact caaacatcaa
    12481 ttcgctgaga tttatcctca aatcaataaa aagattcgct tctactattt aggggttctc
    12541 accattgatt ttctattttt tgttctctta gccttcatta gttctcagcg tttttcatct
    12601 cttatgccaa tcatcactgc ttgccattct actttttatt atatgacagc tgactaccta
    12661 agagaaaact atccagactt ttacgacaaa cacatctcta acacgtatac tattttatcg
    12721 atttcttctt ttttagtata agacagctcg ttctccataa atattcatta actttcattt
    12781 ttatactttt cctttcgatt ttcaaaaaca aggggaggga ttaacctccc ttatattaga
    12841 cataactttt aggatcacaa ttgtatgttc ctttcgaaat atccccaaaa atcgtagccc
    12901 ctgttaaata atgatttctt gtagaagcat atgctatttt cttcatcaca tccagtctcg
    12961 ctacttttag ctcgagatta ggattggaaa acatagcatt tttaatatct tccaaatctt
    13021 gagaactagg tgcgagtaga gttacatcgc cctccaatct attagccttg tctatataaa
    13081 gactaactct ttcagcactt tctgttcgca gaaaatttag tacatttcta ttcatttttt
    13141 tacctcgtat ttttctaatt attgattttt tctagaaaca aaatcaattc aacggtgaag
    13201 ctctagcttt catcttcatt cgtacctcct tttgtgtata tctagcttta cataaaccta
    13261 tgatttagag tatagtttct caattattct cagaaactat ggagatgatt taattatcat
    13321 acaaatcaaa gacctctcct tgctcatctt caccgtaact ctcgagttcc tcataaaagt
    13381 attccaaagc agtttgttct tctggtgtca taaagaaact cctttctaaa caatattagt
    13441 aaaaaatacg ttcttatttc catcctaaat aaagtagtgt ttttatcccg taataggttt
    13501 tatttttgcg gagcattaca ctccgcatta atattgatag tattaagaat cattattact
    13561 taataaaaat tgcgctctca aacatagacg tatacaaaat gccctatgta tacgtttcgc
    13621 ttggaagaca tctaagaaaa aattttgtag ttcagacagt aagattaata catctttctc
    13681 taacataagt gatgtctggt actcccaatt attgctctgt tttaacgatg aaaaaggtat
    13741 agctgtctct tttaatttta tccaatctat acgttctttt ttaggaacgt attctctaac
    13801 agcttgacta acaatactgg aatagcttac ttcatagcct aatttattgt aaacatcatc
    13861 tttaagctta tctaatatct gtctggattc agacgctaaa cgcattctaa cattctcttt
    13921 tcccatatta tcaccccttt ctgacgggat tctgttatcc cgtaaattaa gtatataaca
    13981 atttagaaaa aaaatcaaga attaagttga aaaaaattcc acaatctaaa aatttatcat
    14041 tttatgacaa acatactatc gttaaatgct gatttaatcg aaattttgag ttataaactc
    14101 ctcagtttcg aactcaaaat tagccgtgca ggataggata cccttcataa acttatataa
    14161 ctactttaat accttaaaaa gctaataaaa taaggaatat ctaagcaata tacattctaa
    14221 aatacctgta cgggagtgtc tctaaagaaa aggaggtttt agcatgaaaa aaatcatctt
    14281 catcaaaacc attcaactcc ttgtcattga tggaatcatg ctggcatttt tgacatttaa
    14341 aaggggggct tacttgggac tggattttga tttatagtgg ttggctcatt ttctttcatc
    14401 ctgtgctatt gacctatctt tcaaaccaac tttgtgacca ctttagtcaa ctctattccc
    14461 agattagacc gagattctgg cgttttgctt tacaaattct cctatgggat agcctgatga
    14521 ttctctcctt ggtgtcttta agtgatattc cacttttcct tcagggaact ctcctcatcc
    14581 taggacatct catcccttcc tatcgcatct gccaaagcct gaaaagagac ttcccccaag
    14641 catatcaaga accgatttct ttttggagta ttttatgatc gatgagaaag accaagccga
    14701 ctgggcttgg tctttcttat ctctttttag tatctaggat aatggtaaca ggtccgttat
    14761 taaccagctc aacctgcata tctgctccaa agatacctgt ctgaacgggc acttcttgcg
    14821 ctaatttttg attgaaagca tcatagaagt ctgatgccat atcaggttta gctgcccctg
    14881 taaaggctgg acgattgcct ttcttagtat ccgcaaagag ggtaaactga gaaatagaga
    14941 ggatttctcc ttcaatatct ttgacagaca ggttcatctt gccttctgcg tctgaaaaaa
    15001 tccgcatatt gaccagtttt ctcacagcat agtccaaatc ttcctcttgg tcctctggtc
    15061 caacaccaac cagcaataaa agtccctgat tgatttttcc ctgaatctgg ccttctatac
    15121 tcacttgggc ttttttaacc cgttggataa tgattttcat aatagccttt ctagtaagag
    15181 ctaggacaac tagccgttgg tccgtttgac agagtaaact tctggcacac tcttaatttt
    15241 atcgacaacc gtggtcagtg tagagaggtt ggcaataccg aaggacacat ggatattagc
    15301 aaacttcata tccttggttg gttgggcatt gaccgttgaa atattcttgg ttgtatttga
    15361 aagaacttgc agtacatcgt tcaacagtcc tgtacggttg agaccgtaga tatcgatatg
    15421 ggccatatac tccttatttg agctagagta ctggtcttcc cattccacat caaggagacg
    15481 ttgctcgtag ttttcttggg cacgcaggtt catacagtcc acacggtgaa tagccacacc
    15541 acgacccttg gtaatgtagc caacaatatc gtcaccaggc acggggttac aacacttagc
    15601 aatccgcact aggagaccag aagcaccttc aataaccact cccccctcat gcttgacctt
    15661 gagagtttct ttattttcaa ccttgacctc gccacctttg acaagctcct ctgcctcagc
    15721 cttggccttg gcacgctctt cctcacggcg ttccttttca gtcagacggt taaagacggt
    15781 aatcgcaccg atttccccaa aaccaatggc cgcaaagagg gagtcttctg tcttgtaact
    15841 ggtcttttgc agaacttgat ccatgtggcg cttgtccata aatttatttg ccacatagcc
    15901 attttcttgg aactgagcca tcagcatctc acgacccttg ttgacagaca attccttatc
    15961 ttggttttta aagaactggc gaatcttatt gcgcgccttg ctagtcttga ccatattgag
    16021 ccagtcacgg ctaggtccaa aggagttcgg gttggcgata atttcaacct gatcccctgt
    16081 ctttaacttg gttgtcagtg gaaccatgcg gccattgacc ttggcaccag ttgctttttc
    16141 accgaccttg gtatggattt cgtaggcaaa atcaatcggt cctgaatctt tgggaagaga
    16201 acggacagct ccatctgggg taaaaacgta aatctcctca gccagatagt tttccttaac
    16261 agagtccaca aattccttag catcatcagc ctggtcttgg agctccatca tctccttgat
    16321 ccagttcatt ccaatagctg attccttgct gttaacttgc ccctttatac ctttcttata
    16381 agcccagtga gccgcaaccc cgtactcagc cacctcgtgc atttccttgg ttcgaatctg
    16441 gaattcaatc ggcccttttg gtccataaac agtcgtatgg atagactgat aaccattagc
    16501 cttgcggttg gcaatatagt ctttgaagcg acctggcatc ggtttccaaa attcatgcac
    16561 gtaaccaagc atggcataaa catcactttg ggtatctaaa atacaacgaa tagcaatcag
    16621 atcatagatt tcctcaaacc gttttctctt gtcctgcatt ttgcggaaaa ttgagtaaat
    16681 atgcttggga cgaccataaa tcttcccttt caagtgacgt tctgtcgtat actcctctaa
    16741 ttttgtgact acctcatcca ccaaggcctc acgctccctg cgcttttcct tcatcatatg
    16801 ggtaatcttg taaaactccg ttggattgag ataacggaaa gacaagtctt ctaattccca
    16861 tttgacactg gaaatcccca aacgatgggc aagcggggca tagatttcca tggtttcttt
    16921 ggaaatacgc tcctgcttgt cttttcgaag atgtttcagg gtccgcatat tgtgcaagcg
    16981 gtcagacagt ttgaccaaaa taacgcggat gtcctcagac atggccatga gcatcttgcg
    17041 atgattttcc gctaattgct cctcgatcga tttgtactcg accttgccaa gcttggtaac
    17101 tccgtcaaca atcatccgca catcaggacc aaactctctt tccaaatcgt ccaaagtcgc
    17161 atctgtatct tccaccacat catgcaagaa tccacaagct actgttacag catccagctt
    17221 tagcttagct aaaatacctg ccacttggat agggtgaatg atataaggct cgcctgattt
    17281 gcgatattga ccactgtggc attcaacagc atagaccaag gccttatgga caaaatgaac
    17341 atcctcttcc gttaaatatt ctttggttaa agcgacaact tcttcgcctg ttaaattcac
    17401 ttctttcggc atctctactc tccaattctt cctaccattt tatcactttt ttaagaatat
    17461 gaaaactaga ttggaacaga ataagaaaaa aataattcaa aattgcttga taattctgaa
    17521 ttattggccc ataatatact acgaagttag attttaaact taggtgatag aaggagagat
    17581 agaagaacgg aaaccatatt gtaacccaaa gactttctga tttccccaat tccattgaag
    17641 atacgaaaga taaacggtgg aactcgtatc acatacactg gtaccttgac tggattttgg
    17701 aattaatact caatgaaaat caaagagcaa actaggaaac tagccacagg ttgctcaaag
    17761 cactgctttg aggttgtaga taagactgac aaagtcagtc acatatatac ggcaaggcga
    17821 cgttgacgcg gtttgaagag atttttgaag agtataaaaa tcctcaagat actttcttct
    17881 atcctttagt ttataaggag aatacctatg aaaaaactgc tatttctatc tttgctctcc
    17941 taatgttagg agtttgctgc ctgttcctat tcagccagca aagctataaa aaactcgttc
    18001 aatactatgc taacgaccag aacctgccca gtaggataac ttatagtgaa tatagcgaca
    18061 aatgagaagc caactacggt agcactctaa acatcacgtc tatcaaacaa gctaatgacg
    18121 gagtttatgc aacctatgaa gggcaattga cacctttcca atattgataa attgataacc
    18181 agcctgtctt catctagtca tgctggtttt taagttcatt ttaaatcctt acctattctc
    18241 cctaactgtg ctatacttaa tttatactca atgaaaatca aagagcaaac tagaaagcta
    18301 gccgcaggct gttcaaagca ctgctttgag gttgcagata aagttgacgc ggtttgaaga
    18361 gattttcgaa gagtattagt acattctttg agattggagt tagtatgaaa atccataaaa
    18421 ccgtgaatcc tgttgcctat gaaaatacct attatctaga aggcgaaaag cacctcatcg
    18481 tcgtcgatcc tggtagtcat tgggaagcca ttcgtcagac aatcgagaag atcaacaaac
    18541 cgatctgtgc tattctcttg acccacgccc attatgacca tatcatgagt ctggacttgg
    18601 ttcgcgagac gtttggcaat cctcctgtct atatcgcaga gagcgaagcc agctggctct
    18661 acactcctgt cgataatctc tccggtctcc ctcgccacga tgatatggca gatgtggtca
    18721 caaaacctgc agaacacacc tttgtctttc acgaagaata ccaactagag gaatttcgtt
    18781 ttaaggttct accgacccca gggcactcta tcggtggtgt ttccctagtc tttcctgatg
    18841 ctcacctagt cttgacggga gatgctctat tccgcgaaac tatcggacgg accgaccttc
    18901 cgactggtag catggagcaa ctccttcata gtatccagac ccaactcttc accctaccaa
    18961 actacgatgt ctatccagga catggtccag ctactactat cgctcacgaa aaggccttca
    19021 atcccttttt ctagcaagat gatgacaatc gaaatttaag taaactatcc agcaaatctt
    19081 tctattacaa aaggcatcct atcaaggttt tcacacgtga ttggatgcct tttttctgat
    19141 gactagattt tttgtattac caaataatca cgcgctcctc tggtgaacgc cacattccgt
    19201 ctccttcttt gacatcatag gttgtaaaga aatcgtcgaa gtttggtact tgcacattga
    19261 cacggagttt ggctggtgcg tgcacatcga cgctagccaa aagtttcata aattctggtc
    19321 gacctttcat gcgccagatg cgaccgaagt tgtagaagaa ctcttctgct gagaagtctg
    19381 cttctctctt agctgcttca agcgctgctg cgattcctcc caagtcagcc acgttttctg
    19441 atacagtcaa tttaccgtta atggttgctc cataagaatc ctgtccatca aattggtcaa
    19501 tgactttttg tgttttctcc ttgaaggcag catagtcgct ctctgtccac caatccttga
    19561 ggctaccatt ttcgtcaaag gaagccccgt tagtatcaaa ggcgtgggaa atttcatggg
    19621 caatcactgc cccaatacca ccgtagttag cagaagatga ctgatgcaag tcatagaaag
    19681 gcgcctgtaa aatggccgct ggaaagacaa tcaggttctt ctgaggattg tagtaggcat
    19741 tgaccatatg agcaggcatg ccccattcct tatagtctac aggctggttc cacttactcc
    19801 aactgtgctt gatttccaca cgcgcaaagg ctagagcatt ctcaaaaaga ctggcagttt
    19861 cattcactac cttatccttg taacgtgcag gcaattcttc tggatagcca atataaggtt
    19921 tgatcacatt gagcttcacg atagcctgtt tacaggtttc tggagtgagc cagtcattct
    19981 taagcagacg ctccttatag acatcaatca tggttgccac ttttttctcc acatccgcct
    20041 tggcttctgg agagaacttc tcacgggcgt accaaagccc cagggcttgc ttgaaaggtt
    20101 cctgtgctag atgataagct gctttgacct tatcttttgc ctctggaact ccagaaaggg
    20161 cacggctgta ggcaccagac aaaacacgga tatcctctgt taaatagctg gttgaaagat
    20221 tgacaacact caaaatcaag gttgctttaa ggagagacca ggcttcctca ctgtaaaatt
    20281 gctctgctgc ttgccagaaa cgttcctcgt ctacaataac cttgtctggt aattgcccaa
    20341 taactgcttt gaagaagtca tccaaaggta gggcaggcgc gaatttcttg aaatcttcgt
    20401 aagaatatgg atgatagagt ttagcatatt ctgaactttc ttcattagag agcaccactg
    20461 ccgcaactcg gcggtccaat tcaagtcttt tttctagcaa gtcttcaatt tcttcatcag
    20521 agaaatcata agccttgagg agatttgcgc tgctttcttt ccaaagagtc aagagctctt
    20581 cgcgctgagg atgttcttct gcatagtagg tcgtatctgg caagattgtg cttggagcgc
    20641 tagcccatag aacattgatt ctagcatcca taaagtctgg cgatacacca aaaggaagga
    20701 agtttggttt tcctgcaagc tcaaactctg ctagtttagc tgtaaaatcc gcaaaagttt
    20761 ccaattcttg gaattcttta aggagtggta agacaggtgt gataccgtca gcttctctct
    20821 tgtcaaaatc acgaactagg cggtggtatt tgacaaagtt ttccaagata gcatcctcag
    20881 gcacttcttc acctgctaac cacttgtctg ttgtcgccag catcaggtct tcaatttcct
    20941 ggtctaaatc aacaaaacct cctgtttgag acttatctgc tgggatttca gctgtctgtt
    21001 gccattctcc attgatagca tcataaaaat catcttgata acgtgtcatc ttgttctcgc
    21061 tttcatttgt atttgcattt atcttaacaa aaatcactag ggaatgcaat taataaccat
    21121 ttaactatta ttttttagct attataaaaa acctttccat gataaaacgc ataattccaa
    21181 gttttttaca cttgatacta tgcgttttat aatttagaaa tttatcctga aagttatctt
    21241 tagaatctat tttt
//
LOCUS       DAVBCN010000030        21251 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVBCN010000030 DAVBCN010000000
VERSION     DAVBCN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21251
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.030"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(295..2022)
                     /gene="ezrA"
                     /locus_tag="VQ497_001060"
     CDS             complement(295..2022)
                     /gene="ezrA"
                     /locus_tag="VQ497_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HET2517377.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2104..4050)
                     /gene="gyrB"
                     /locus_tag="VQ497_001061"
     CDS             complement(2104..4050)
                     /gene="gyrB"
                     /locus_tag="VQ497_001061"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET2517378.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFIPDPKIFTETTTFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4065..4628)
                     /locus_tag="VQ497_001062"
     CDS             complement(4065..4628)
                     /locus_tag="VQ497_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2517379.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4713..5267)
                     /locus_tag="VQ497_001063"
     CDS             complement(4713..5267)
                     /locus_tag="VQ497_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET2517380.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5278..6501)
                     /locus_tag="VQ497_001064"
     CDS             complement(5278..6501)
                     /locus_tag="VQ497_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET2517381.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVSLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6538..8988)
                     /locus_tag="VQ497_001065"
     CDS             complement(6538..8988)
                     /locus_tag="VQ497_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HET2517382.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQAMGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIIGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9187..10116)
                     /locus_tag="VQ497_001066"
     CDS             complement(9187..10116)
                     /locus_tag="VQ497_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET2517383.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10256..10543
                     /locus_tag="VQ497_001067"
     CDS             10256..10543
                     /locus_tag="VQ497_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HET2517384.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10577..11911)
                     /gene="ciaH"
                     /locus_tag="VQ497_001068"
     CDS             complement(10577..11911)
                     /gene="ciaH"
                     /locus_tag="VQ497_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HET2517385.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(11901..12575)
                     /gene="ciaR"
                     /locus_tag="VQ497_001069"
     CDS             complement(11901..12575)
                     /gene="ciaR"
                     /locus_tag="VQ497_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HET2517386.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12684..15230)
                     /locus_tag="VQ497_001070"
     CDS             complement(12684..15230)
                     /locus_tag="VQ497_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET2517387.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHIAYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15354..15794)
                     /locus_tag="VQ497_001071"
     CDS             complement(15354..15794)
                     /locus_tag="VQ497_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HET2517388.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15769..16638)
                     /locus_tag="VQ497_001072"
     CDS             complement(15769..16638)
                     /locus_tag="VQ497_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HET2517389.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFTELTNTEANCLVGTKEFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDLESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16680..17408)
                     /locus_tag="VQ497_001073"
     CDS             complement(16680..17408)
                     /locus_tag="VQ497_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2517390.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            complement(17416..17895)
                     /locus_tag="VQ497_001074"
     CDS             complement(17416..17895)
                     /locus_tag="VQ497_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2517391.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVDHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVK"
     gene            complement(17906..18604)
                     /locus_tag="VQ497_001075"
     CDS             complement(17906..18604)
                     /locus_tag="VQ497_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HET2517392.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18597..18863)
                     /locus_tag="VQ497_001076"
     CDS             complement(18597..18863)
                     /locus_tag="VQ497_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HET2517393.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(18929..19855)
                     /locus_tag="VQ497_001077"
     CDS             complement(18929..19855)
                     /locus_tag="VQ497_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HET2517394.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19930..20745)
                     /locus_tag="VQ497_001078"
     CDS             complement(19930..20745)
                     /locus_tag="VQ497_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517395.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20749..21069)
                     /locus_tag="VQ497_001079"
     CDS             complement(20749..21069)
                     /locus_tag="VQ497_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET2517396.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLTKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
BASE COUNT         6426 a         4669 c         3717 g         6439 t
ORIGIN      
        1 aagttacttc aaagtacaga tttgaagctg cagataagac tgacgaaatc actttaatac
       61 taaatgaaaa tcaaaaagca aactaagaag ctagcctcag gctgtacttg agtacggtaa
      121 ggcgacgctg acgtgatttg aagagatttt cgaagagtat aaaacactgt tttgaagtgg
      181 tggataaatc ggctgggtaa aaactcaatc tcaggagaaa atgaagtaaa tctccccaca
      241 actatagaaa aagatttgat tctgctcctc acacaataaa atctttttct tttattaaaa
      301 acgaatcgtt tcacgtgttt tctcatatga ggtaacaaag cgattggtta caccaggctc
      361 tgccacttcc aatgcttgag aaatcttgtc aaatgaagcg tgataatcaa attctttttc
      421 aaaaatatct aaagcttcgt taaatgcttc ttgaatgcgt tcatcaaatg agcgatagcg
      481 gttagaatat tgcaagagtt gctctgtcaa agttgcatat tgtacaatat tataagtttc
      541 cgtttctaaa gcttccatat cattcgttgc aatttcaaga actcgggtaa cagattcaat
      601 gttaatcatt ttttgttcta actcaaccat taaatcctcg gtattattgc ttgccgtaaa
      661 gaataacttc aagaaagttt gtggaatacc tggcagattg cgtttttcca tgtatcgctt
      721 gatagtatgg agacgattga cataaacatt ggccttttga cgtgcattaa tatcatcttt
      781 ctcaatttgt gtcaggcgct cactaactga aatttgctca tcttcaatat cttttagttg
      841 agtttgtaaa tcctcaagat tttcttcaag aactgaataa gcttgggttg gttcttcttg
      901 atttgaagtt acctcaacaa tagctgcctc aaaactctct aattctgtct gaatacgacg
      961 aacatggctt gcagctgtct caggaagtaa ataggtcttg ttcaaacgtg caatatcttc
     1021 tcccaataaa gtattattct ctttcatatg ttgaaggtaa gttggaagag ttgcaagtag
     1081 attttccact actttctgag cagcaatttc tcgagtaaaa atatcataca aggcattgat
     1141 ttcctcttgt gcctgtccat tctcatattc ggcattatcc aattccaact gacgaatatt
     1201 ctcttggttt ttcttgaatg cttcataaag caagtggaaa cgcgcttcaa tatccgtttc
     1261 aacaaaatga taattagcat caattagttt acgataaccg gcttccaaat cctgtaattg
     1321 atctggcaat tctgtagaaa gcgtcgtaac caaggctgga acacgatcca caatatgact
     1381 taaggccaaa atgtgatttt ctgtattatc caaaatcact gcggcttcca cagggtcacc
     1441 cgatgaattc aaggttacaa attgtgaaaa ttcagattgg atattttcta attgtttttc
     1501 aatttcatcc aaggcttgac catactgttc tgaattttca gcaactctat gctgaagttc
     1561 ctcaaataaa tccaaagcat gaagaacacg accactattt ttagattctt gcttctctaa
     1621 gtctgccaaa gcattgcgaa ttgccgcaat atcttcttca atcaaagtaa tttgactctc
     1681 aatttggtca atttgatgac tggccttgag aaaacgaaat gaatggttat aaccttctgc
     1741 ttcaaagaga ttattttcaa tatcggcaaa agagttgaga gataaatcga cccatttttg
     1801 attccattca cggaaagcca cttgactttg tccaatcaag tgcatatttt ttacagcttc
     1861 tacttcatca tttactggaa gattgtatag ttcttctttt ctttcttcta gcgcctctaa
     1921 tctcccctcg tttcgcttac gtagaaagat tgccactaca taagccagaa ctaaaatgac
     1981 tgcaattgca accattaaat aaattagttg tccattagac atatcaaact ccttttttac
     2041 ttgaaacaat cgtaacgatt atatcatatt ttttagacca agggaactac ttctccctat
     2101 tttttagaca tcaagtgtac tatagacagc attttcttcg ataaactcac gacgaggctc
     2161 tactcgatcc cccatcaaca tatcaaagat tttatctgct tctgcagcat catctacaga
     2221 aactctagcc atcaagcgat gttcgggatc catggttgtt tcccacagct gatgatcgtc
     2281 catttcacct agccccttat aacgctgaat agtcggtttg gtacgacctt cactataacg
     2341 ggctaaagct tcttggagtt tgatttcttg atctgcaccc ggctggatat attctttaat
     2401 ctcgcttcca accttgacac catagattgg tggttgggca atataaacat aaccagcttc
     2461 taggattggt ttcatataac gataaatcaa ggttaaaaga agggtacgaa tatgggctcc
     2521 atcgacatcg gcatcggtca tcaaaacgag tttttggtaa cgggctttcg aaacatcaaa
     2581 ttctgcgcca aatcctgttc ccatggctgt gaaaagacta cgaatttctt cgttagctag
     2641 aatcttatcc atacttgctt tttcaacgtt caaaatctta ccgcgaattg gaaggatagc
     2701 ctgaaactca cggttacgac cagatttggc tgatccacca gctgagtctc cttcgacgat
     2761 gaagagttct gtttcagcag ggttattaga agaacagtct gctagtttcc ctggaaggtt
     2821 ggaaatttcc aaaccagatt ttttacgtgt gacttcacgc gcacgcttgg cagccacacg
     2881 agccttggca gctaaaatcc ctttttccac gatacgtttg gcaatctgtg gattttccat
     2941 gaggaaatcg gagaaggctt cactgaagag gcgattggta atcttgacca cttcgctatt
     3001 tcccaatttg gtcttggttt gtccttcaaa ctgtggattt gggtgtttaa ctgagataac
     3061 tgcagttaat ccttcgcgaa catcttcccc tgttaaatta tcttcattgt ctttcagtaa
     3121 cttattctta cgagcataat cattgataac acgagtcagt gccgtacgga aaccttgttc
     3181 atgcgttcca ccttcatggg tatgaatatt attggcgaaa ctcatgacat tttcatggta
     3241 acctgttgtg tactgcatgg ctacctcaac tgtgatatca tccatctcac cgtctgtata
     3301 gattggtgta tcaaagatta catccttgtt ctcgttgata tattcaacgt aactagcaat
     3361 cccaccttca taatgataat gcttggtttg ttccaaacct tggcgcttat cagtgataga
     3421 aatttgaaga ccgcgattta gaaaggccaa ctcttgaatc cgtttattta atttatcaaa
     3481 atcaaaggtt gttgtttcag taaagatttt tgggtccggt atgaagtgaa ccgttgttcc
     3541 agttctatct gtatccccaa ccacctcaag gtctgcaaca acatgaccac gacggtattc
     3601 ttggtaatga atcttaccgt ttttatggac atgaacgtct aattgagtgg aaagggcatt
     3661 aactactgac gaccccaccc cgtgaagacc acctgaaacc ttgtatccac caccgccgaa
     3721 ctttcctcca gcatgaagga ctgtaaagac ggtctcaaca gcaggacggc ctgttttttc
     3781 ctgaatatcg actgggatac cacgcccatc atccacaaca gtaatcgaat catctggctc
     3841 aataaaaact tgaatatggc tggcaaatcc tgccaaggcc tcgtcaattg agttatcaac
     3901 aatttcccag actagatggt gaagaccttc ttttgaggtt gatccaatgt acatccctgg
     3961 acgcatacga acagcctcta agccctctaa aacttgaatt tgactggcat cataatcctg
     4021 tgcctgcaga tttttgattt cttctgtcat cttattcctt tttcttatat gtctaatact
     4081 tgtcttaaat tcacgatact ggtaaacaag tgggtatcaa gtcctgcagc ttgacctgct
     4141 tcgatatcaa tcggccgatc accaatgaca agaccagagc taatctgata cttttctctt
     4201 aaataaagca tggattcggg atttggcttt ctcttaaagc ctgagctaga agtcaccact
     4261 tctgtaaaat aagctgctat agaggttttt tctaaaattt ccaaaacctg atcatttcga
     4321 tgagagacca aaaaatgacg gccaccttga tttaaaatgt cttccaatag gtcagaaact
     4381 ccttcaaata aaatcgggtg ttcaagctct ctggcttcat tttccttgta cttttctaaa
     4441 aaattctcta aattgggagc gaatgtctca atcgcaaaag gagtagaaac ctttaaagct
     4501 tgatagacac tgtcatggtc ttgtgtgata ccatacagtg ccaatgtttc aacaaatgca
     4561 gctgttgaag tttcataatt atccagtaaa gttccaccta aatcccagat gtaatcgtga
     4621 tatttcatac ccttcattat agcatttttt tatgaaaaaa gcgatgattt ccctgagttt
     4681 tccacataaa aaacgagaga ataactcccg ttttactgat ttttaccaaa gacatctcga
     4741 tagagcattc ctgttcgtaa actctctgcg tcccctccta gttgctccac caactcgtag
     4801 gcaaaggcaa gggctgttga aggaccccga ctggttgtca actgaccatc taccactact
     4861 gtttccttga cgtagtgacc atcaaggatt tgctcttgaa cgccgtcata acaagtgtat
     4921 cgcttatttt tcaatatctc tgcttgattg agggcaattg gtgccgcaca aatggctgct
     4981 agtttcttcc cttcttgctc gaagctttgc aattcttgaa tcaaggtctg attatcacgt
     5041 aaatgtgcag aaccaggcat acctccagga agaacaatca tatcatagtc tgataaatct
     5101 ccatcaaaga catgatctgc tcttacttgg attgcatgcg aacccgttac ttgctcttca
     5161 aaaccaacca tatcacatgt gatattggct cgacgcaaga catctacaac tgtcaaggct
     5221 tcaatttctt caaagccctg agctaacata actgctactt ttaccatgat tttctcctta
     5281 tttaatttgt tttaatacaa cctttttccg tttgaatggg acttttccgc tcttttcttc
     5341 agctagatta gtctggtaac tcatcgataa aagcaaacca acaccaatca gattactgat
     5401 aatagctgat cccccttgcg aaatgaaagg caagggaatc cccgtcaaag gaagtagtcc
     5461 agtcacagca ccgatattct caaagatgtg gaagagcaac atcataatca aacctgtgga
     5521 aatataagtg tagaactggt tatttgattt aagagtaatc ttcaacatac ggtaaatcaa
     5581 catgagatag agggcaataa ccaggacaga gccaataaag ccaaaatctt ctgcaataac
     5641 cgtaaaaatc atatctgact ctcgaactgg gataagcaga ttcgaagcat taaatccctg
     5701 accaaataag ccaccactcc caatggcaat ctgcccttga gcctgctggt aagtcgttgt
     5761 ttgggcaaac tcaaagggat tgagccaagc caaaatccga ttgatttggt aggtcggcat
     5821 tccaatctgg tgaagaaaag ctcgtccgtc cttgctaata aagatagcta agaaaccagc
     5881 aactcctgtt acagcagtca caaatactgg gataataatt ttccaagaaa cccctgataa
     5941 taaaacgatt cctgagaaaa tggctacaaa aaccaaagcc gtccccaagt cactttgaag
     6001 tgctaaaaga actaggactg gaatggtaaa gagaatcatc cagaaaatta acaaaaagtc
     6061 cagcgaaacc gtgcgtctcc attccttatg tttctttgta aattggacaa tgacacgagc
     6121 caacatgagg atataggata tcttcataaa ttctgacggt tgaaataggg taattccatt
     6181 tattgatacc cagtttttgg cacccgttga tgcaactaag cttggattat aaaatacaat
     6241 cggcaagatc ataagtccca agcctaaaat atatagaaag ggggtcacct tccaaagaaa
     6301 ttctgtatta aagagcatga ccacaaaacc aatcacaagc cccaaggcaa tccaggcgac
     6361 ctgctgccct aaaatgggca gaatattatt gggataatca tgactaacgg ctatatagat
     6421 agccaccaca ccgatgacca gtagaaaaaa tactggcaag agcaaactat aatcgactct
     6481 agagtcgaga gaacgtttca tatatactaa ccttatactt tcatacaata ctatttatca
     6541 aagttcatta aaaaatctat caatagcctc gtcaacttcg gatcgagaga tggttttaac
     6601 agtcgcttct tctgctagag atgctactat ttgtttgccg tatcgttttc cgatgattct
     6661 cctatccaaa ataagagtta aggaacgttg gtattcacgt ctcatacttc ttcccaaagc
     6721 ctgttttaaa cgaataatgg ccattggcaa ttgataatca taaaaggcat ttttcccttc
     6781 ttgattcagt tcttgattaa tctttttcgt caagggttct tgaggatttt ggaaaggaag
     6841 cctcggtaca acttgaatca caaaaggatg gcttgaaaaa tcaactccct cccagaaact
     6901 tgctgcacca agcaagattt gttgttcacc tttttcaaag cgtttcttta gctgatgaac
     6961 atccccattt ttatactggg ccaagtggct aactgtaagt aaatccgata ctgctagaag
     7021 catgtcttta gcggtaaaga gaaccaaaat cggttgctgg aaagcttgaa tttccactag
     7081 taaagcagct acctctctgg catagacttc taaggaggtt tctgttacca gagggaaatc
     7141 tttgaccata accacttctt gttcctgttt tccccgagat tcaatcttga caaatttggc
     7201 ttcaggatag cctaaaaggt ctgccaaaga aaccctctga ctaatctcaa gagtagccga
     7261 tactcccatg gcttggcaac tctcaggaag taaagaggac agaagagtac ggcctgattt
     7321 tgtagaagaa atctgaattt tcttttgact cgtttcagtt acttcaagcc agtaatcacc
     7381 ttcagctgta aaatagcgaa ccagctcatc aaagccttct acttctaatt ctgaaaaata
     7441 ctgatggaga ttgtccagag aatctaagat atttttccta gatttaccag actgaaattg
     7501 ttctatcaag tagagacact caaagcgaat actttctagt atccgttgtt gaaccctgtt
     7561 ttcttctcct actaaagcct tatcaattaa atcgataata gattgtatat cgtaggtctc
     7621 ttgaagcaga ttttctagag ctaacaaaat cttttggact tcatcaataa tcagtaaacg
     7681 gtcactgaca aattcaggat tatcttcaag tctggtcacg agataggcat gattagtcac
     7741 taaaagcttg caagtctctg ccctttcttg actacgtttc caaaaatctt ccgtcacaaa
     7801 taagctctgg gatgataaat tcccatcatg acgaaggtct gctagaaaat gttggtaacg
     7861 atagagttgc ccgatttcat ccaaatctcc tgtctctgtc tcagtaagcc agaccaagac
     7921 ttgcatttta aagcgtctaa ataagcgatt ttcatcattt tcctgcaagg aatgataaaa
     7981 ggcatccaac ttcagataat tttgtggtcc ctttaagcta tgaatatctg tatggaacac
     8041 ttccttgagg cgtttacctt cttcttccat gatttgattt tgaagaatct ttgtcggaac
     8101 actaagaaca atttgtcgct ctttggattg agataaagcg ggtaagagat agccataggt
     8161 tttcccaatc cctgtcggcg cttgaatcag agagacaggt tcatctttca atagcaagcc
     8221 aacctcttta gcaaaacttt cttgttcctc cctcacttca aggttcaaca gagaaatatt
     8281 tttagaaaag tcttgagata gttttcgtgg ctccagagaa gctgccgttt tcttaaaata
     8341 cagaccttga acttggacca agtctggaga actcaggata gattggttgc gataagtttc
     8401 ctcaataacc aggtaggact catataggag agcgtcagcc atttccagca agcgttccaa
     8461 gagaccttta ggaagctgag tcatcttttc ccgtaaaaaa agaagtaatt ctgctgtagc
     8521 ttgggcatct gaaagggctg tgtgtgcgtg tttaagagga attcctaatt ctcgacacaa
     8581 aatcggcaag ctatattttt ccagttcagg gaaaaagacc tgggccaatt cgaccgtatc
     8641 aacacgaggg tttcttagct catagccttc aaaaaataaa ttttccgcca agagattagc
     8701 atcaaactga acattatggg ctacaaaaat cccatcctcc accaagtcaa atatttttct
     8761 ggcaacttgc gaaaaatcag gtgcttgcgc cagacgttgg tctgtcaatc ctgtcagttc
     8821 tttgatatga gcatccaagg gttcatgtgg attgacatcc gtcgtatagt gatcgacgat
     8881 ttctccgtcc tcaatcacga caattcccac ttggataatt ttagccttac tacctgtgct
     8941 agttgcctct aaatccacca caacatagcg attaccaatc gttttcatta taaaaaatta
     9001 taccaaaaaa agggccattt ggcacctgca aacataggat aattttcaaa ctcaagaaca
     9061 ctacttttct taaaaatccc atcgaataga gcattctagc ctgctgattt tttaaaatag
     9121 tgaagaaata cgaaaaattg agagaagaaa tgttttcatc ttccttctct caacgaatca
     9181 tgtattttat ttaaccatgt caggatcgta gtcataaaat cctgtatcaa ggaaacggtt
     9241 tttagggtga agtttacgaa cttcttcatc cagcaagaag gcttggtcat ggctaaagtt
     9301 gccctcttga atcaagctac gcgcttggat aaagagtttt gcaatctcaa caaagttctg
     9361 accaggagtg tagagccctt ctagtttcca gcgagtaaag ccatgctcca ccaattctgt
     9421 taatttgatc atcaaatcaa ggtcattgtt ggcaaagata tgggtcccat gattatcttc
     9481 aaaaatggaa tagtggctct ctggatcact tggctcagcc aagaagaggt cacgtttatg
     9541 cgtcttttca tcatcgatat gtgtaaagtt atagtagttt tgcaagagtg gacgtttaga
     9601 atgatggatg acgctagcac cgtaaaccaa aacttcagca ggaatttcca aaatctctgg
     9661 cattttgaaa agttcagctg atggaatttc acgcgccaaa acagcctcag atgcgccagc
     9721 cttttgtccc cagaagttaa tctgacgact gctagttacc atggttgaag catcgtagat
     9781 ggtcttaaat gaataaccat cgcggttaac tacgtaaaag acgcctgcat ccccaatcgt
     9841 aatatagtct gtcttgattt cttccaagaa gtctaagaaa ggcttgatac ggtccatcat
     9901 atcttggtgc atgagagcat tgaccgcaac gagcaattcc ttaccagcat catgaaccaa
     9961 cttagcgatt tcacgtaatt ggtcataact aaaggtcgtt ggcagacgaa ggccaaaatc
    10021 tttctcaccg acatagatac ggtctacgcc agcttcgagt agttgttcaa cttgttcaat
    10081 actttcagca gttgctgtaa tgacaatctt ttccataaga aaaattatac cacatttctc
    10141 caaaatcatc catgctttga aaatgaaaag ggactttatt ctttttgtaa cctttctgta
    10201 ataattctct gccgaaaaaa tgataaaata ggtatgtact gttaaggaga gaattatgcc
    10261 cgtaagaaaa ttacaatcct atgaggtaga ctatcaagaa gaattaaacc agcagcttcc
    10321 tcattaccaa gcttatacac ctgaagcgca atctgatgcc aatctcaagg aaatcctatt
    10381 ttttattaat atcgctgttt tttgtatctg tattgctatc tttagtttta tctttttagc
    10441 attaaaatta tcaactgctc ttgcctttgc cgcagcaatc ggattcagct tacttgtttt
    10501 aaaagttcaa cggtctatta tcaaacgaaa acgtagaaga taaatccaaa atatcgcccc
    10561 agttggagcg atatttttat tttttctttt tagatggtgt ctgaatggca atcttcactt
    10621 caaagattgt tcccttgggt ttattatctt tgacagtaac agttcctttt agagcatcta
    10681 caatttgctt ggctagggat aatcctaaac caaaaccacc tttttgccga gttctagcct
    10741 tgtctactcg ataaaaacgg tcaaaaattt tctttttatc ttctgtcgaa ataccgattc
    10801 cattatcaga aacaagtaaa taaagattgc gatcggtcgc cgagataaga aaatcaattt
    10861 caccatcctc ctcagtatac ttgacggcat tatcgaaaag aatggtcatc agttgtttca
    10921 gaagaagctg atctgtgaca attgttcgat ggatacgatt ttcaaaacgg aagacacgat
    10981 tattttccga agcaatcatc tcgtagtttg tgaaagttgt attaaaaaag ctagttggaa
    11041 cttctgcaag ctccggctta atcccatcat ctctccgagc taagttcagc aagcttgtcg
    11101 ttaaaaaacg catatttcgg acttcttcca aactcgatgc aatgctttcg ctcacatcca
    11161 taatggtagc ttctggctta cgaaaaaggg tctctaagcg attttgcaaa actgcgagtg
    11221 gagttcgtaa ctcatgactg gcattttcca caaaagactg ttgcttctgc atactctcaa
    11281 gcaggggcct aacactgacc ctagctagat agagactggc aagtaaagac aaaatccaga
    11341 aactagccat cacgaccaca atcaattgct catgcttttg actagcctgt tccaactgac
    11401 tggtattaat caagacagca gcatacttga tattggttga aaccgaacta atattggtct
    11461 ccatcaaaat cacacgatag atttcttcct gcccatagct attaaaaacc tgaatctggt
    11521 agatatgtcc tagttctttc ttttctaact taatcttatc caagcccaaa aatcgatttc
    11581 cagaaagaag ttgagtaaag tctttatcaa agagaatgac ttccgtattg gaactgacat
    11641 taggttttat ttctacttta ctagcgtccg ccctagcatt ttctaaatct ttaatctctt
    11701 ctgttgccct atttatagcc agctgaataa ctgcttgagg attttcactc agtccatgaa
    11761 gcttatcgtc caccgaagta taaagactcg aatgcatgac ttgtaaaata atcagagtca
    11821 ttgtagaaaa aatcagggtg aagacaccga agttgcggat aaaataacta aagtcatccg
    11881 cataccatgt ttttttaagt ttactgaaca tcttttaaaa gatacccaac actacgcaaa
    11941 gtttgcaaat tctctgcaaa agtggttccc tttaatttct tacggacttt tgaaacatag
    12001 acttcgacaa ccgaaatcgt tgtatcacta tcaaatcccc atagacggtc aaaaatctgc
    12061 gtcttaggca aaatcacatt ttgattttga aggaaataaa ctagtaaatc gaactctttc
    12121 cccagcaatt cgacaggagt atcttcaact ttaacggtat tggttgataa attaaccacg
    12181 atattcccat aagtcaaggt gttttcatta aacttccctg aacgtttgag aagggcctga
    12241 atccgcattt taagttcttc taggtagaaa ggtttggtca gataatcatc cgctcccagt
    12301 tcaaatccat gtcccttgtc atccaaactt tccttggcag tcataatcag aactggtgtc
    12361 gtaattccct tttcacgcaa ttcttttaag acttggaaac catttttttc tggcaacatc
    12421 aaatccagca aaatcaagtc atagacacca ctctcagctt cgtagagacc ttcttctcca
    12481 tcaaatacct gcataacatc cgcaaaatcg tctaaaaagt caaatactga atttgacaga
    12541 cctaggtcat cctcaaccaa taagattttt atcatgagaa actcctcctt attaaaacta
    12601 ttataccaaa tttgccttaa aaaaaactca actctctgca ttttacatga gatagctgag
    12661 ttttcttttt attttaggct tatttatgca tttccgtatt gaagaacaac tgcttcgact
    12721 gcagcttttt cacggctaat caagtcaaca cgcgctgcaa tttccttgat tcccataccg
    12781 atgttacggc taagagcaag gtcagaaagt tgcggctcaa agaactcctt gtattccgcc
    12841 aagcgttgct gagtcttaaa tacatgagca ggaaggataa caaagctatc aaagctcata
    12901 tctcctccaa gggctgcctt aatccaagcc cagttttcac gcgcccaaga ccaagctgtt
    12961 ttctgagttg cttgatgagc taggaattgg taataccaag cagacaagtc ctgtggtttg
    13021 accacaaatt tgtccttcca agaagtaatc aagttttgga tattatccgc atctgtactg
    13081 tatgcaagag ctgctgccaa ctggcgttta aagacagcat ctgttgcgtg agtataagta
    13141 tcaagataaa gtgctaacaa gtctttagtc tcatgatgtt tcatctcatt aatcagaact
    13201 tgtgaacgaa tagctgctgg gagtcctgca agattctcct tgtgtgttgc gaagatttgg
    13261 ctagcgactt gactagcttc tgcatcattt gagcgaatca tcatcgaaac agccagctga
    13321 cgaaccaatt catcctcatc tgattctccg tctttagctt caaaaccaag acggtcatag
    13381 ttatgacgag ccaatttagc aaccagtcct ttgaaggctg tttcagcatc cgttccttca
    13441 tcaataaagc gctcaagggc tgaaatcact tgagaaacag ctgaaaccac cagataagac
    13501 tcttccttag caagtttatc aagaactgga agcaagtctg cataagcaat gtgccctgcc
    13561 tcagccaaca aacgacgttc ttgaacaatt tgcagtttgc ttgtgttatc aagtgtctct
    13621 agctcagcaa gaacagcttc taacaagtct ccttgatagt cggtaatata gtgggcagta
    13681 ttttcagtgt tgagacgaag agctccttca ttttcagcaa gaagagctgc gtagccaggg
    13741 atttcgatac tttcagtttc gagtgtatca ggcaagcctt tccagttgct attgaggggc
    13801 accacccaga gacggttctt gtcttcgttc tcaccgatga agaattgttt ttgtgaaatc
    13861 ttcaagacat cattttcaac tttaacagta agaactgggt aaccaggctg ttccaaccaa
    13921 gaatccatga aggctgcgac atcacgtcct gacgcttgac caagggcatc ccaaaggtca
    13981 ctaccaatgg tgttgctgta ttggtgtttt tcaaagtagg catgcaaacc tttagcaaaa
    14041 tcagcatctc ctagccaacg gcgaagcatg tgcatgagac ggcttccttt ggcatagacg
    14101 atagcgccgt caaagagtgt attgatttca tctggatgtt taacttcgac gtggacagac
    14161 tgaacgccat cagtagcgtc acgttcaaga gcaagaggta ctccacctgt ttggaaatct
    14221 tcaaagatat tccagcttgg ttcgatggta tccacacaga cgtattccat catattagcg
    14281 aaactttcat tgagccaaag gtcatcccac catttcatag tcacgaggtt cccaaaccat
    14341 tggtgagcca attcatgggc cacaacaagg gcaacttgtt gacggctagc aaatgtagag
    14401 ttctcatcga caaccaagta aacttcacgg taggtcacaa gaccccagtt ttccatagca
    14461 ccagctgaga agtcaggaag ggcgatgtgg agagattgag gaattgggta cttaactcca
    14521 tagtaatctt cgtaaaactc gatagagcga acagcgatat ccagtgagaa atcaagattt
    14581 gaaagtggat gtgctttggt tgagtagaca cctaccaggg taccattttt agttttagcg
    14641 gtcacccctt gcaaatcacc agcaacaaag gccaacaagt aagaagacat gcgaggtgtt
    14701 gtctcaaact tccagatacc tgtttcctta cggttttcaa catcgatttc tggcatgttt
    14761 gacaaggcca attcaccttc tgcttggtca aagcgaagag agaggtcaaa agttgctttg
    14821 gcttcaggct catccacaca tgggaaagct tcgcgcgcaa aatggctctc gaactgagta
    14881 gacaagacct ccttcttgac tccatcaact gtataataag aagggtaaat ccctgtcatg
    14941 ttgtctgtaa ttttaccaga aaaggcaaga accaattcaa cttgaccagc ctcagccaat
    15001 tcgatatgaa gggcttcatt gtcatggtca actgtaaatg gacgagcttg acctgcaact
    15061 tctacagagg tgatttccaa gtctttttgg tggagggaga tgcggtcact ctgtgcttga
    15121 ccagtgatgg tcactttccc agaaaaagtc ttggtctcac gactcaaatc taaaaataaa
    15181 tcataatgtt caggaacaaa ttgcttaata aaatgttcaa ctgcttgcat agttttctcc
    15241 tattctaagt ttaagagcta ctgctcttct tcaaacaaac ttattatatc atattttgct
    15301 tgagaaaaaa ggattggaca gcgatttcca aaactttctt ccttctaaat agtctacagt
    15361 gggtagacct tgcgaaattc ttctaaaaca accttgcttt caggtgtaaa agtcagccct
    15421 acttcaccca aacagtctgt gaaaaagtcc tcatgaacct ggcgccaata ggcaagtgat
    15481 ttgtctccct caccttcctt aaaggcatgt tgcgcagaaa cttgattgaa gagttcaaca
    15541 gaaacctttg taatttcgac aatgcagaca gactgatttt gactatctaa aatgacatcg
    15601 aaggtcccta cttggggaag tggttcgtct tctagtacat agaggtcata ggctgatgct
    15661 gttgctgtct tttctccttt aaacaccaaa tccgctaaaa ggtctggttc aactccaaaa
    15721 gcccaagcat cgatttcatc tccgatcaaa ggattgattt gcttgtattt attccacatt
    15781 tcttgcggta tcatgggtac tcctttgtaa ttttttactt tcttctttta tgtgtttaag
    15841 atgatctgga tggtcaatct ctaaatcaaa aatctctgga atagaactgt agtggataat
    15901 gcacttgata cccaactgat tcattttttg tatgaaagaa gtattcagat agcctgctac
    15961 agcaaaatta atcttgttct ttcttgcttt atcctgcata tctcttagca tatctaacat
    16021 tattggactt tccagatcat gccattgact gtttctcata gtcgcaaaaa caaaggaagt
    16081 caaatcattc attccaacta caatctttga aatgcccgtt tccagtatac tagataagtc
    16141 aaaatacgct gacggtaatt caatcatcgt tccgactttc ccagtaaagc catactgacg
    16201 caatactgta atagcttgtt ttaattggtc ggcatcattg acaaaaggaa agataacaga
    16261 tagattgggg ttggtttgat aaacttctgt aacgacatgt gcttcagcct gaaattcatc
    16321 caaacacgcc agtaaacgcc tggttcctct atagccaaac aagggatgcc cttcgtcaaa
    16381 aaactcttta gtccccacta aacaattggc ttctgtattc gttaattcag taaaacgata
    16441 ccaaacttct tcatctgagt acagggagca gatagtctct agataatctt ttacaaattg
    16501 ctgacaactt gctaatatga tgttttgatt aagctctctc aacaaatatt ccccacgaat
    16561 catgccgacg tggtgaaata gttgaggata aattttttca agaatttttt cgccactaag
    16621 ggcaagttga tttctcatag ccattcacct cgtcttaaaa gatacaatat catatataat
    16681 tatatcaaaa tttcctctag ttcaaaagct tcataaggtt cagataactc atatcctaaa
    16741 aattctgcta cttttttgga agcctcatta tgagcatccc aaagtggaaa catatctcta
    16801 tttaaactct ctagaatcat tgcagcacca agctttttag caaatccatt tccttgttcg
    16861 tttggtctag ttgcaacttc cacttcaact gctttacggt aaactaaccc tgaggaaatc
    16921 ccagcaatca gttcattatt tttaagaatc acaaagccaa atccaccttt taaaacaaaa
    16981 tcctgatagg actcaaaatc cccctgtaaa tcctgtgacc attcttccgt agaaaagcag
    17041 ttataaatat gattatcaat aggcacaata ttgtaacctt cctctaaatg agtaactaga
    17101 ttatttaaaa attcaacttg aaaatttgcc ttatctttaa aagaatagcg agtaaaagaa
    17161 tttagcccca cttttcgatg cagaaaatct tgccaattag tattttctga aataataatc
    17221 ttataatcta aaccatattt tctaacaaaa tcttcccaaa atctggaatc caactcccct
    17281 gctaggtata gaaaattccc aacatcataa aagcttgttg tcctatcatt gtttcggtag
    17341 acagttccaa tttgagattt aagaccatag agaaccatat ttttcttcca tgttttaaat
    17401 agttccatta tccctctact tcaccttcca attcatgtaa gaagtcttgt ccagttctgg
    17461 aaatcctaat aattcagact taaccttcaa gactaatggc gatgcatttt cttctgtaat
    17521 ctcttgaata tccatccaaa tatatccaag tgaatcattc gcaccatcag acacagcttc
    17581 cgaagtcgta acttgaggtg cactctcatt catttcaaca tcatacaagg ccatgacatg
    17641 gtcaaccata aaatttgtta actcttctct gacgaaaaca tcgtagattc gaggattaga
    17701 gtagcttcta acagtaaatc ccgtctcttc cataacttct ctagtcagcg tttccgtcag
    17761 tccttcacca agttgctgac tgcctccagg tagatcatac cgatgttgat aagggcctct
    17821 cgttttttca atgcagagta acttcccatt ttctaagcaa acagcgtaga ctccaaagtg
    17881 ttttttgatt tccatccaac tcctcctact tcaaagacca gccaccatct attgtcaaga
    17941 tttgtccttg catggcgctc gcttttccac ttgctaaaaa aagactaagc tctgctattt
    18001 cctctggctc aatccagcgc ttgattggag tttcactagc cacccagtca gccaaaccac
    18061 ctggttcaaa atccgcagcg gtcatagctg tcttgactgc tcctggagcg ataccaaaga
    18121 cctgaatccc agcttcagca tagtctagag ccaactgctt ggtgaagcca gccaaggcat
    18181 gcttggatga agtataggcg tgaccacctc cacctgctag gctagaagca atggaacaca
    18241 tattgatgat gattcccttt ttattttcca gcatttgtgt caaataatac cgagtcaact
    18301 ctactggaat aatgtagttg atttcaaaaa tctcttgaat gtcctgcgcc gtttgttcca
    18361 acagtggttt gtaatcatcc aaaactccag cagtattaca caaaacatcc acctgagggc
    18421 accagtcaaa aataggttcc aagtccaagg tcaaatctct ctgtaaaaag tgaaaatcac
    18481 cctctaagag tggcttttca ccttggtcaa ctccataaac ttgatagccc ttctctaaaa
    18541 agaggcgagc ttgagccaat ccgatccctg aactcactcc tgtaatgagt acacgtttag
    18601 tcatgcactt ctacccaatc cgttgccaaa acatcacaaa ctgtcgggct ccacatggaa
    18661 aaaccttctc cttcgccaga aacgttgatt aggaaataag gtgtcatttc aagtgcaagc
    18721 ccattttgct cgatggtatc aaagagttgg acatagtttt ccgcacctcc ccaaccagtt
    18781 cgtacatatt ttctcttagc ctttaaccca ggcaggatct cttcaaatgt catgtttttc
    18841 tcctttaatt ctacattctt catttaatta tagcaaaaaa ccgctttata cggctttttg
    18901 aatgtgagtt attcaaacct gctactactt acggcaaatt attccctgca gcaagataaa
    18961 tttcatacca ttcttttctt gttaagctaa agtttgccgc tcggctaact tctctcaagt
    19021 gcttaggatt tgttgtacct acgactgcct gcatttttgc tggataacgc aatatccaag
    19081 aaatggcaat agttgaagag gttactccat atttaatagc taaacgatca agtacttgat
    19141 ttaaagcttg aaatttctca tttccaacaa aattcccttt aaaatacccg aattgtaaga
    19201 cagaccatgc ttgaatgacc acatcgtgta attggcaata ttcaaaaatg ctgccatctc
    19261 gcatagctgc ttgactatct tccatattaa catgaaaagc tgattcaaat cctggagtaa
    19321 aagccgcact caattgtagc tgattaacag ctaacggctg cttgacatct tttttaagca
    19381 actccatcat cataggattt tgattagaaa ctccaaaatc tcgaacttta ccttgtttat
    19441 aaaggagatt aaaggcttct gctacttggt cagattccat caaagcatct ggtcgatgaa
    19501 ggagcaagct atctagatga tcaatcttca atctttgcaa aataccgtct actgatttta
    19561 taatatagtc cttagaaaaa tcaaaatagg taaattcttc aatgcgaatg ccacatttgg
    19621 actgaatcca catcttttct cttaaatctg gacgattttt taggacaaga cctaacagtt
    19681 cttcacaacg accacgacca taaatatcag ccaagtcgaa ggcattgatt ccaacagaaa
    19741 gtgctgtttc tacaagctct tcaacttctt ttacagactt atcttttatt ctcatcattc
    19801 cgagaacaat ttctgataat tctttgtcat cttgaccaag agttatgtat ctcatcaaat
    19861 ttttctcctt taatttctaa cattcttccc ttcattataa caaaaaaccg ctttgcaacg
    19921 gctttttgac tatacttcac tccattttat cttcttaaac ccacggaaca agacaaagat
    19981 tccaataaag aggacagcta aaggaataac ttttgtaagg aaaacatttg aaattcccat
    20041 ccactcataa taacggagca gagaacccac cacaagatgg gcaataatca tactgacaaa
    20101 tggacgaaag accgcttctt tccaattcca aataccgata actagcgaaa tcgtaaagac
    20161 agacaaacta tcccagggag ccggaatata aaaggctcct tcttgtatga agcttgccat
    20221 tcctacatat cctaaaacaa ctagaagaac tatagtccca acaacaatgt aagtgccaat
    20281 tttcatttta ggagaatctt ggactaaact tcttcgtaaa attgtggcca caagtccaaa
    20341 tccaatcaga aaaataagaa gttgccctaa aaatgtgagc aaattgactg ttaagagagg
    20401 acctttagaa aaatcactta gtagttgata ataacgtaat acagccagga caagaattgg
    20461 cgtcaaaagg gactctttga tagaactgcg aggtgctccc ttgagaatct ctttcattat
    20521 ttttttagga ttcttaccta gataatcctc tgcactcatg ccatctcgtt ctgcttctga
    20581 gaaatctagc atcatcaaat agatctgctc tctgagatag tcttcatcat agagaaatcc
    20641 agcaagatta aaactttccc acaactcctc aaaatacttt tgattctcct cagaaaactc
    20701 atgtagcaaa gcgcttgttt cttcgtaata cttcattttc ttcatggttt aacccccatt
    20761 cttaatccct tctacttttt gactcaaatc gtcccattgc tgccaaaaga ctgagacacg
    20821 ctcttctcct tctttcgtta atgaaaaata cttccgatct ggaccatctg gcgacgggcg
    20881 catgtcgcct cttatccatt gatttttttc taacttttgc aacaaaggat aaatagttcc
    20941 tggaacgata gtatcaaatc cagcctctcg caaagtctga accaactcat aaccataccg
    21001 ctctttttga ccaatcatat ccaagacaca accttcaaga acacctttta atagctgagt
    21061 ttctttcatc acttctccct tctaatctat tttgtaatac ctactagtaa cttcacctat
    21121 agtatattac ttctacacta gtttgtaaag cataatagtt aatactcttc gaaaatctct
    21181 tcaaaccacg tcagcgtcgc cctaccgtat gtatggttac tgacttcgtc agtttcatct
    21241 acaacctcaa a
//
LOCUS       DAVBCN010000031        20578 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVBCN010000031 DAVBCN010000000
VERSION     DAVBCN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20578
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.031"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            128..2110
                     /locus_tag="VQ497_001080"
     CDS             128..2110
                     /locus_tag="VQ497_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2517397.1"
                     /translation="MKSSKLLALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDDQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQGSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            2411..7714
                     /locus_tag="VQ497_001081"
     CDS             2411..7714
                     /locus_tag="VQ497_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HET2517398.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATANENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLFSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKTPGNSTWYKGNRVVAPEPGSVNRLSITLKSDGQLNASNNDVNLFDTVTLPGAVN
                     DNLKNEKKILLKAGTYGNDRTVVSVKTDNQEGVKADDTPAQKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVNDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGQGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWNGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLVSDQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALFLASVSLALSALFVVKTKKD"
     gene            complement(7881..10040)
                     /locus_tag="VQ497_001082"
     CDS             complement(7881..10040)
                     /locus_tag="VQ497_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HET2517399.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGIYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(10037..10633)
                     /gene="recU"
                     /locus_tag="VQ497_001083"
     CDS             complement(10037..10633)
                     /gene="recU"
                     /locus_tag="VQ497_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HET2517400.1"
                     /translation="MINYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLTVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            10699..11226
                     /locus_tag="VQ497_001084"
     CDS             10699..11226
                     /locus_tag="VQ497_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HET2517401.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            11296..11625
                     /gene="gpsB"
                     /locus_tag="VQ497_001085"
     CDS             11296..11625
                     /gene="gpsB"
                     /locus_tag="VQ497_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HET2517402.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            11646..12026
                     /gene="rnpB"
                     /locus_tag="VQ497_001086"
     ncRNA           11646..12026
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VQ497_001086"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            12111..13268
                     /locus_tag="VQ497_001087"
     CDS             12111..13268
                     /locus_tag="VQ497_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HET2517403.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARLPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVERKLELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            13281..14675
                     /gene="mapZ"
                     /locus_tag="VQ497_001088"
     CDS             13281..14675
                     /gene="mapZ"
                     /locus_tag="VQ497_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HET2517404.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTNSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLVTQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            14751..16175
                     /gene="gndA"
                     /locus_tag="VQ497_001089"
     CDS             14751..16175
                     /gene="gndA"
                     /locus_tag="VQ497_001089"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HET2517405.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVSDVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEDQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            16187..16876
                     /locus_tag="VQ497_001090"
     CDS             16187..16876
                     /locus_tag="VQ497_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2517406.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVFIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            16974..17930
                     /gene="cbpC"
                     /locus_tag="VQ497_001091"
     CDS             16974..17930
                     /gene="cbpC"
                     /locus_tag="VQ497_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HET2517407.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDDLFDNRPVFEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLRSSGAMA
                     TGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSSGAMATGWYQDGSTWYYLNAGN
                     GDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(18048..19280)
                     /locus_tag="VQ497_001092"
     CDS             complement(18048..19280)
                     /locus_tag="VQ497_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2517408.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALCIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVHIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLFALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFVTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(19323..19910)
                     /locus_tag="VQ497_001093"
     CDS             complement(19323..19910)
                     /locus_tag="VQ497_001093"
                     /inference="COORDINATES: protein motif:HMM:NF017000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517409.1"
                     /translation="MTILLKLSSTIVYGEIYHYFLQRDTAKESILDYSFAHGYCEIAY
                     ALFAYSKVLEPSMFYNDLHTFHAELKKLLEKVTSNTENLGNLQLSWCEGISGIILYLC
                     MYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKIQQVILA
                     CSERDDHGLLMFQGDSGKADLFDFGIGSRGYIGVY"
     gene            complement(19810..20146)
                     /locus_tag="VQ497_001094"
                     /pseudo
     CDS             complement(19810..20146)
                     /locus_tag="VQ497_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic-modifying protein"
BASE COUNT         6416 a         3718 c         4601 g         5843 t
ORIGIN      
        1 cggttacgat attcatattt tttgcaaaga tgttatttga aaaataattt tcaaaaattc
       61 tgaaaattct gttgacaact ttctgaaaag agtctataat ggagagaaag ttttaaagga
      121 gaaaaggatg aaaagttcaa aactacttgc ccttgcgggc gtgacattat tggcggcgac
      181 tactttagct gcatgctctg gatcaggttc aagcactaaa ggtgagaaga cattctcata
      241 catttatgag acagaccctg ataacctcaa ctatttgaca actgctaagg ctgcgacagc
      301 aaatattacc agtaacgtgg ttgatggttt gctagaaaat gatcgctacg ggaactttgt
      361 gccgtctatg gctgaggatt ggtctgtatc caaggatgga ttgacttaca cttatactat
      421 ccgtaaggat gcaaaatggt atacttctga aggtgaagaa tacgcggcag tcaaagctca
      481 agactttgta acaggactaa aatatgctgc tgataaaaaa tcagatgctc tttaccttgt
      541 tcaagaatca atcaaagggt tggatgccta tgtaaaaggg gaaatcaaag atttctcaca
      601 agtaggaatt aaggctttgg atgatcagac agttcagtac actttgaaca aaccagaaag
      661 cttctggaat tctaagacaa ccatgggtgt gcttgcgcca gttaatgaag agtttttgaa
      721 ctcaaaaggg gatgattttg ccaaagcgac ggatccaagt agtctcttgt ataacggtcc
      781 ttatttgttg aaatccattg tgacaaaatc ctctgttgaa tttgcgaaaa atccgaacta
      841 ctgggataag gacaatgtgc atgttgacaa agttaaattg tcattctggg atggtcaaga
      901 taccagcaaa cctgcagaaa actttaaaga tggtagcctt acagcagctc gtctctatcc
      961 aacaagtgca agtttcgcag agcttgagaa gagtatgaag gacaatattg tctatactca
     1021 acaaggctct attacgtatc tagttggtac aaatattgac cgtcagtcct ataaatatac
     1081 atctaagacc agcgacgaac aaaaggcatc gactaaaaag gctctcttaa acaaggattt
     1141 ccgtcaggct attgcctttg gttttgatcg tacagcctat gcctctcagt tgaatggaca
     1201 aactggagca agcaaaatct tacgtaatat ctttgttcca ccaacatttg ttcaagcaga
     1261 tggtaaaaac tttggcgata tggtcaaaga gaaattggtc acttatgggg atgaatggaa
     1321 ggatgttaat cttgcagatt ctcaggatgg tctttacaat ccagaaaaag ccaaggctga
     1381 atttgctaaa gctaaatcag ccttacaagc agaaggtgtg actttcccaa ttcatttgga
     1441 tatgccagtt gaccagacag caactacaaa agttcagcgc gtccaatcta tgaaacaatc
     1501 cttggaagca actttaggag ctgataatgt cattattgat atccaacaac tacaaaaaga
     1561 cgaagtaaac aatattacat attttgctga aaatgctgct ggcgaagact gggatttatc
     1621 agataatgtc ggttggggtc cagactttgc cgatccatca acctaccttg atattatcaa
     1681 accttctgta ggagaaagta ctaaaacata tttagggttt gactcagggg aagataatgt
     1741 agctgctaaa aaagtaggtc tatatgacta cgaaaaattg gtcactgagg ctggtgatga
     1801 ggctacagat gttgctaaac gctatgataa atacgctgca gcccaagctt ggttgacaga
     1861 tagtgctttg attattccaa ctacatctcg tacagggcgc ccaatcttgt ctaagatggt
     1921 accatttaca ataccatttg cattgtcagg aaataaaggt acaagtgaac cagtcttgta
     1981 taaatacttg gaacttcaag acaaggcagt cactgtagat gaataccaaa aagctcagga
     2041 aaaatggatg aaagaaaaag aagagtctaa taaaaaggct caagaagatc tcgcaaaaca
     2101 tgtgaaataa ctgttgcaaa atataagaaa ggatttagta tttctcttga atgctgaatc
     2161 cttttttaca tttgtaaaga aagattctaa atgtactgac ccccaaaagt tggagcctct
     2221 ttttgtcaga atagagaaaa tttttgttaa ttttacttgt ttcctattgc tttctcagct
     2281 attatttgtt atattaaaag tacaattatt ttttatttat cagggttaag cattgcactt
     2341 tcagaggaag gagtattttt taaaaaggaa atgtaaacgc ttgctaaaaa atgaaaggat
     2401 ttagaagttt atgaataaag gattatttga aaaacgttgt aaatatagta ttcggaaatt
     2461 ttcattaggt gttgcttctg ttatgattgg agctgcattc tttgggacaa gtccggttct
     2521 tgcagatagc gtgcagtctg gttccacggc gaacttacca gctgatttag ctactgctct
     2581 tgcaacagca aacgagaatg atgggcgtga ttttgaagcg cctaaggtgg gagaagacca
     2641 aggttctcca gaagttacag atggacctaa gacagaagaa gaactattag cacttgaaaa
     2701 agaaaaaccg gctgaagaaa aaccaaaaga ggataaacct gcagctgcta aacctgaaac
     2761 acctaagacg gtaactcctg aatggcaaac ggtagagaaa aaagaacaac agggaacagt
     2821 cactatccga gaagaaaaag gtgtccgcta caaccaacta ttctcaactg ctcaaaatga
     2881 taacgcaggc aaaccagccc tgtttgaaaa gaagggcttg accgttgatg ccaatggaaa
     2941 tgcaactgtt gatttaacct tcaaagaaga ttctgaaaag ggcaaatcac gctttggtgt
     3001 cttcctgaaa tttaaagata ccaataataa tgtttttgtc ggttatgaca aggatggctg
     3061 gttctgggaa tacaaaactc caggtaatag cacatggtat aaaggcaacc gtgtagttgc
     3121 accagaaccg ggttctgtaa accgtctttc tatcactctc aagtcagatg gacaactcaa
     3181 tgccagcaat aacgatgtca atctctttga cacagtgaca ctgcctgggg cggtcaatga
     3241 taatcttaaa aatgagaaga agatccttct caaggcagga acttatggca atgaccgtac
     3301 agttgtcagc gttaaaacag acaatcaaga aggcgtaaaa gcggatgata ctcctgccca
     3361 gaaagaaaca ggtccggttg ttgacgatag caaggtgact tatgatacga tccagtctaa
     3421 ggtcctcaaa gcagtgattg accaagcctt ccctcgtgtc aaggaataca gcttgaatgg
     3481 acatactttg ccaggacagg tgcaacagtt caaccaagtc tttatcaata accaccgaat
     3541 cacccctgaa gtcacttata agaaaatcaa tgagacaaca gcagagtact tgatgaagct
     3601 tcgcgatgat gctcacttaa tcaatgcgga aatgacagta cgcttgcaag ttgtggacaa
     3661 tcaattgcac tttgatgtga ccaagattgt caaccacaat caagtcactc caggtcaaaa
     3721 gattgatgac gaaagaaaac tactttcttc tattagtttc ctcggcaatg ctttagtctc
     3781 tgtttctagt gatcaaactg gtgctaagtt tgatggggca accatgtcaa acaatacgca
     3841 tgtcagcgga gatgatcata tcgatgtaac caatccaatg aaagacctag ccaagggtta
     3901 catgtatgga tttgtttcta cagataagct tgctgctggt gtttggagta actctcaaaa
     3961 tagctatggt ggtggttcga atgactggac tcgtttgaca gcttataaag aaacagtcgg
     4021 aaatgccaac tatgtaggaa tccacagctc tgaatggcaa tgggaaaaag cttataaggg
     4081 cattgttttc ccagaataca cgaaggaact tccaagtgct aaggttgtta tcactgaaga
     4141 tgccaatgca gacaagaaag tcgattggca agatggtgcc attgcttatc gtagcattat
     4201 gaacaatcct caaggttggg aaaaagttaa ggatatcaca gcttaccgta tcgcgatgaa
     4261 ctttggttct caagcacaaa atccattcct tatgaccttg gatggtatca agaaaatcaa
     4321 tctccacaca gatggtcttg ggcaaggtgt tctccttaaa ggatatggta gcgaaggcca
     4381 tgactctggt cacttgaact atgctgatat tggtaagcgt atcggtggtg tcgaagactt
     4441 caagacccta attgagaagg ctaagaaata tggagctcat ctaggtatcc acgttaacgc
     4501 ttcagaaact tatcctgagt ctaaatactt caatgaaaaa attctccgta agaatccaga
     4561 tggaagctat agctatggtt ggaactggct agatcaaggt atcaacattg atgctgccta
     4621 tgacctagct catggtcgtt tggcacgttg ggaagatttg aagaaaaaac ttggtgacgg
     4681 tctcgacttt atctatgtgg acgtttgggg taatggtcaa tcaggtgata acggtgcctg
     4741 ggctacccac gttcttgcta aagaaattaa caaacaaggc tggcgctttg cgatcgagtg
     4801 gggccatggt ggtgagtacg actctacctt ccatcactgg gcagctgact tgacctacgg
     4861 tggctacacc aataaaggta tcaacagtgc catcacccgc tttatccgta accaccaaaa
     4921 agatgcttgg gttggggact acagaagtta tggtggtgca gccaactatc cactgctagg
     4981 tggctacagc atgaaagact ttgaaggctg gcagggaaga agtgactaca atggctatgt
     5041 aaccaactta tttgcccatg acgtcatgac taagtacttc caacacttca ctgtaagtaa
     5101 atgggaaaat ggtacaccgg tgactatgac cgataacggt agtacctata aatggactcc
     5161 agaaatgcga gtggaattgg tagatgctga caataataaa gtagttgtaa ctcgtaagtc
     5221 aaatgatgtc aatagtccac aatatcgcga acgtacagta actctcaacg gacgtgtcat
     5281 ccaagatggt tcagcttact tgactccttg gaactgggat gcaaatggta agaaactttc
     5341 tactgataag gaaaagatgt actacttcaa tacgcaggcc ggtgcaacaa cttggaccct
     5401 tccaagcgat tgggcaaaga gcaaggttta cctttacaag ctaactgacc aaggtaagac
     5461 agaagagcaa gaactaactg taaaagatgg taaaattacc ctagatcttc tagcaaatca
     5521 accatacgtt ctctatcgtt caaaacaaac caatcctgaa atgtcatgga gtgaaggcat
     5581 gcacatctat gaccaaggat ttaacagtgg taccttgaaa cattggacca tttcaggcga
     5641 tgcttctaag gcagaaattg tcaagtctca aggggcaaac gatatgcttc gtattcaagg
     5701 aaacaaagaa aaagttagtc tcactcagaa attaactggc ttgaaaccaa ataccaagta
     5761 tgccgtttat gtcggtgtcg ataaccgtag taatgccaag gcaagtatca ctgtgaatac
     5821 tggtgaaaaa gaagtgacta cttataccaa taagtctctc gccctcaact atgtaaaagc
     5881 ctacgcccac aatacacgtc gtaacaatgc tacagttaac gatacaagtt acttccaaaa
     5941 catgtacgcc ttctttacaa caggatcgga cgtctcaaat gttactctga cattgagtcg
     6001 tgaagctggt gatcaagcaa cttactttga tgaaattcgt acctttgaaa acaattcaag
     6061 catgtatgga gacaagcacg atacaggtca aggaaccttc aagcaagact ttgaaaatgt
     6121 agctcaagga atcttcccat ttgtagtggg tggtgtcgaa ggtgtcgaag acaaccgcac
     6181 tcacttgtct gaaaaacacg atccatatac acaacgtggt tggaacggta agaaagtcga
     6241 tgatgttatc gaaggaaatt ggtcactcaa gacaaatgga ctagtgagcc gtcgtaactt
     6301 ggtttaccaa accatcccac aaaacttccg ttttgaagca ggtaagacct accgtgtaac
     6361 ctttgaatac gaagcaggtt cagacaatac ctatgctttt gtagtcggta agggagaatt
     6421 ccagtcaggt cgtcgtggca ctcaagcaag caacttggaa atgcatgaat tgccaaatac
     6481 ttggacagat tctaagaaag ccaagaaggc aaccttcctt gtgacaggtg cagaaacagg
     6541 ggatacttgg gtaggtatct actcaactgg caatgcaagt aatactcgtg gtgattctgg
     6601 tggaaatgcc aacttccgtg gttataacga cttcatgatg gataatcttc aaatcgaaga
     6661 aattacccta acaggtaaga tgttgacaga aaatgctctg aagaactact tgccaacggt
     6721 tgccatgact aactacacca aagagtctat ggatgctttg aaagaggcgg tctttaacct
     6781 tagtcaggcc gatgatgata tcagtgtgga agaagcgcgt gcagagattg ccaagattga
     6841 agccttgaag aatgctttgg ttcagaagaa aacggctttg gtagcagatg actttgcaag
     6901 tcttacagct cctgctcagg ctcaagaagg tcttgcaaat gcctttgatg gaaacttatc
     6961 tagtttatgg catacatctt ggaatggtgg agatgtaggc aagcctgcaa ccatggtctt
     7021 gaaagaacca actgaaatca ctggacttcg ttacgttcca cgtggatcag gttcaaatgg
     7081 taacttgcgt gatgtgaaac ttgttgtgac agatgagtct ggcaaggagc atacctttac
     7141 tgcaactgat tggccagata acaataagcc aaaagacatt gattttggta agacaattaa
     7201 ggctaagaaa attgtcctta caggtactaa gacttacgga gatggtggcg ataaatacca
     7261 atctgcagcg gaactcatct ttactcgtcc acaggtagca gaaacacctc ttgacttgtc
     7321 aggctatgaa gcagctttgg ctaaagctca gaaattaaca gacaaagaca atcaagagga
     7381 agtagctagc gttcaggcaa gcatgaaata tgcgacggat aaccatctct tgacggaaag
     7441 aatggtggaa tactttgcag attatctcaa ccaattaaaa gattctgcta cgaaaccaga
     7501 tgctccaact gtagagaaac ctgagtttaa acttagctct ttagtttccg atcaaggtaa
     7561 gacgccagat tataagcaag aaatagctag accagaaaca cctgaacaaa tcttgccagc
     7621 aacaggtgag agtcaatctg acacagccct cttcctagca agtgttagtc tagccctatc
     7681 tgctctcttt gtagtaaaaa cgaagaaaga ctagtattta gtaaaacctc ttaacaagat
     7741 tacggaagca gtctctatct tttccaatga ggtttatagt acagaaaaag cctgagaaga
     7801 catcttctca ggcttttgtt aagcacataa atacaatagt gctatgacaa aatcacccag
     7861 aaaaatctgg gtgataaatg ttatggttgt gctggttgag gattctgatt ttgttgatca
     7921 ggggttgtat ttgattgttg cgtattattg ttaggattgg tagtcgtact attatttgtg
     7981 cttggagtgg ttgagctaga ctgtgaagtt gaactatctg atgatgagct tgaactttca
     8041 gttgatgggg gttgttgtgg agcaggtgag ctccacgtag aacgagcacc atttttaaat
     8101 acgaattctc catttctgta gagcccctct ggtatattcc aatcctctgg attgcttcct
     8161 tcagacaggt aggtcatcat agagcggtaa actttggcag cgaccgtaag gccattgcct
     8221 acaagtggtg tcagacggtt agaatagcct gtccatacag ccattgaata tttacgcgta
     8281 tagccagcaa atagttcatc aggtgctaca aattgagagg tcttgatgtg gttttcaatt
     8341 tcctcgtctg tatagttaga ggttcctgtt ttaccagcct gagggagcca agcaagatag
     8401 gcatttcgtc cagttccata agtcaagact gttttcatca tgtcggtcat catataggct
     8461 gtcgtttcct tcatggcacg agttccgaca ttagagaact ctttttcact cccatcacta
     8521 aagacgactt tatggatata cattggttta tagtaagttc caccatttgc aaaggcagcg
     8581 taagcagcag ccatcttttc actacttgct ccatattttt tgtctgattc ggttgtgtta
     8641 cttgaaatgg catttgagta gtgaatactt gggtagtcga ttccgagacc atttaggaaa
     8701 gtcttggcgc ggttgagtcc gaccttgttt agagtttcca cggctgggac gtttcgcgat
     8761 tgttgcaggg cgtattgcaa ggtgatgttg ccaaagtagc ccctatccca gttataaaca
     8821 ggggtatttg tcccagggta gttatagggc tcatcgtgaa cgatagtagc agttgaatcg
     8881 tagataccgt actccaaggc aggagcatag tctgtgatcg gtttcatagt tgatccccag
     8941 tcgcggtttg tttctactgc ttggttaatt ccgaaggaaa cattacttga ctgatggcgt
     9001 gctcctagct gggcaatgac tttaccgtta gaaacatcaa caatggtaga agcgacttgc
     9061 aattcatcgt ctggataggc aacgtattcg tctgtattgt aaatatccca cagatgtttt
     9121 tgagcttctt ggtctacatt tgtgtagaca tccatcccag ttgtgagcag gttatagcct
     9181 gtttcttctt caacttgatt gatgacttcc ttgaggtaat tatccatgta agcagggtaa
     9241 ttacttgctg atttgagact ttgtagtcca tcagtaattg gtgtattgac tgctttctca
     9301 tactgttcag cagagatgta gccttgattt ttcatttcag ataagaccaa gtttcggcgg
     9361 tcttgggctg cttctggatg tgaatagggg tcatattggt ttggtgcctg aggcattcca
     9421 gccagcaagg ctaactgagg taaacttaaa ttattgaggt ctttaccata gtagttttga
     9481 gctgctgtct gcattccata gttcccatta gacatgtaga ccttatttat atagtaggtc
     9541 aagatttctt gcttggttgc tttttgttct aactgaatcg ctaaccaagc ttcctgagcc
     9601 ttacgagaaa tagtctggtc ggaagtcgaa gttgaaaagt aagtcaactt aatcaactgt
     9661 tgagtgagag ttgatccacc ttggagggaa ttgctttgca gattgcgcaa gaaagctccc
     9721 aggatacgga tggtatcaat ccccctgtgg tcgaagaagc gatggtcttc gatagaaacg
     9781 attgctttaa ccaaatctgt gggaatatca ttagcttggg cattgacgcg gcgttcagaa
     9841 cccaagtcag caatgagttg atttttattg tcgtagattt tactagaagt tgttgcaact
     9901 agtttactct cggataggct aggagccttg ctaacgtagt agaaaaaaac tcctccgcct
     9961 aagacaatgg ctgcgataac caagcttaag aagctaatgc tcagatactt gattaggcgc
    10021 agaatcgttg gtttgttcat cttgttttac cacctaataa atgttctttg ataacattga
    10081 gataaggaat ttgagggaag gcaccagcct tgatttcata tccatattct cgaatatatt
    10141 caagtggcat tgatttttgt cccttatctt gatgatagaa gcgaatcaaa tcgaatgccg
    10201 gcaataagta ggtttcttgc tgagaagaaa agtgaagaag gacaaagcag attccttgtt
    10261 gggcaaggac ttgttccata tgctgaatct gatgtggatg aaaatttttc atcggaatcg
    10321 cacgtttttg ttttgtttcc ttgacttcaa agtcgatgta atatccatta taaacgccag
    10381 aatagtccgt cgttgaagct tgtcgaaaat aggcttcaac aatcttggca cgacttcgtt
    10441 gtggatagtc cacttgtacg atttgaatag gagttggttt cttatgtata acagtcaagc
    10501 cctgagacaa atagtagtcg ttggtagcat tgatcatctt ttcaaaagac attcctcgat
    10561 ttgcgaaatt tttgggttga gaaagagatg tttgtctttt ttgtgatgaa actttatgtg
    10621 gatagttgat cataattctc cttattggta caataacatc actttattat accataattt
    10681 gcacagaaag ggttaaaaat ggctacagct ttggttttag gctattctgc ttttgatttg
    10741 ggtttatttt ctgataagga tcctcgtctt aaattgatta aaaaagcgat acgtaaagat
    10801 ttagaggcta tggcagcaga tggggtgtct tggcttgtgt ttacagggag tttgggtttt
    10861 gaatattggg ttttagaggt tgctcaggag atgaaaaccg aatacggttt ccagttggcc
    10921 accatttttg cttttgaaac ccatggggaa aattggaatg aaggcaatca gatgaaactg
    10981 agtcgcttta agcaggtaga ctttgtcaaa tatgcttatc ctcgctatga acacaagggg
    11041 cagttaagag attaccagca gtttttgctg gaaaacacga ctagttccta tcttttttat
    11101 gatgaagaaa atgaaacaaa actagcttat ttttaccaaa agatgaaaaa tcaagaggac
    11161 tattttataa agagattaac atttgatcaa ttaaatgaac ttgctgaaaa tttttccgaa
    11221 aattgaagct ttgaccttga tttttgtttg ccttttttta tataataata ctagcaagcg
    11281 agaatggaga gagacatggc aagtattatt ttttcagcga aagatatttt tgaacaagag
    11341 tttggacgtg aagtccgtgg ctataataaa gtagaagttg acgagttttt agacgatgtc
    11401 atcaaggact atgaaaccta tgctgccttg gtcaagtcac ttcgtcagga aattgcggat
    11461 ttgaaggaag aattaactcg taaaccgaaa ccttcaccag ttcaagcaga accccttgaa
    11521 gcggcaatta caagttctat gacgaatttt gatattttga aacgcctgaa tagattggaa
    11581 aaagaagttt ttggtaaaca aattttagat aactcagatt tttaagtagt tatttgagat
    11641 gtgcaatttt tggataatcg cgtgaggaga attgcttctc atgaggaaag tccatgctag
    11701 cacaggctgt gatgcctgta gtgtttgtgc taggcgaaac cataagccta gggacgagaa
    11761 atcgttacgg cagttgaaat ggctaagtcc ttggataggc cagagtaggc ttgaaagtgc
    11821 cacagtgacg gagtctttct ggaaacagag agagtggaac gcggtaaacc cctcaagcta
    11881 gcaacccaaa ttttggtcgg ggcatggagt atgcggaaac gaacgtagta ttctgactgc
    11941 tatcagctag agctgttagt ggtagacaga tgattatcga aggaagtggt cctagtcact
    12001 tctggaacaa aacatggctt atagaaaatt gcatataggt tggggctgag aaattttctc
    12061 aacctcattt tttaaagtgg acatatagaa aggtcttgca agactgtaac atgaaaaaag
    12121 aatttaattt aattgcaact gtggcagcag ggcttgaggc tgtcgttggt cgtgaagtgc
    12181 gagagttggg ctacgattgt caggttgaaa atggacgtgt tcgttttcaa ggagacgtga
    12241 gagctattat cgaaaccaac ctttggcttc gggcagcaga tcgtatcaaa attatcgtag
    12301 gaacgttccc agctaagact tttgaagagc tatttcaggg agttttcgct ttagattggg
    12361 aaaattattt accacttgga gctcggttac cgatttcaaa agctaaatgt gttaagtcca
    12421 aacttcacaa tgagcccagt gttcaggcta tttctaagaa agctgttgtc aagaaattgc
    12481 agaaacacta tgctcgccca gaaggggttc ctctgatgga gaatggccca gagtttaaga
    12541 ttgaggtctc tattctcaaa gatgtggcaa ctgtcatgat tgatacgacc gggtctagcc
    12601 tctttaaacg tggttatcgt accgaaaaag gtggcgctcc tatcaaggaa aatatggcag
    12661 cagccatttt acaactttct aactggtatc cagacaagcc tttgattgat ccgacctgtg
    12721 gttcggggac tttctgtatt gaggcagtta tgattgctag aaagatggcg ccaggtcttc
    12781 gtcgctcttt tgcatttgag gaatggaact ggatcagcga tcgcttgatt caagaagtgc
    12841 gcacagaagc ggctaaaaaa gtagaacgta agcttgagtt ggatatcatg ggctgtgata
    12901 ttgatgctcg catggtggaa attgctaagg ccaatgctca ggcagctggt gttgcaggag
    12961 atattacttt taagcagatg cgcgtgcagg atttacgttc cgataaaatc aatggagtaa
    13021 tcatttccaa tccgccttat ggtgaacgtt tgtcagatga tgcaggagtg accaagctct
    13081 atgctgagat ggggcaagta tttgcaccgc tgaaaacttg gagcaaattt atcctgacta
    13141 gtgatgaagc ttttgaaagc aagtatggta gccaagcaga taagaagcgt aagttataca
    13201 acggaacctt gaaagtggat ctatatcaat attttggtca gcgtgtcaaa cggcaagagg
    13261 taaaatagaa agggatactc atgagtaaaa aaagacgaaa tcgtcataaa aaagaaggtc
    13321 aagaaccgca atttgatttt gatgaagcaa aagagctaac agttggtcaa gctattcgta
    13381 aaaatgaaga agtggaagca ggagtcttgc ctgaggattc cattttggac aagtatgtta
    13441 agcaacacag agatgaaatt gaggcggata agtttgcgac tcgtcaatac aaaaaagagg
    13501 agttcgttga aactcagagt ctggatgatt taattcaaga gatgcgtgag gctgtagaga
    13561 agtcagaagc ttcttcggag gaagttccat cttctgaaga catcttacta cccttgcctc
    13621 tggacgatga ggagcaaggc ttggatcctc tattgctaga tgatgaaaat ccaacagaaa
    13681 tgactgaaga agtggaagag gagcaaaacc tttctcgtct ggatcaagag gactcagaaa
    13741 agaaaagtaa aaaaggcttt attttgaccg ttttggcgct tgtatcagta attatttgtg
    13801 tcagtgctta ttatgtctat cgtcaagtgg ctcgttcgac caaggaaatt gaaacttctc
    13861 aatcaactac agccaatcaa tcggatgtgg atgattttaa tacactttat gacgcctttt
    13921 acacaaatag caataaaacg gctttgaaaa atagccagtt tgataaactg agtcaactca
    13981 agactttact tgataagctg gaaggtagtc gtgaacatac gcttgccaaa tctaaatatg
    14041 atagtctagt aacgcaaatc aaggccattc aagatgtcaa tgcacaattt gaaaaaccag
    14101 ctattgtgga tggtgtgttg gataccaatg ccaaagccaa atcggatgct aaatttacgg
    14161 atattaaaac tggaaatacg gagcttgata aagtgctaga taaggctatc agtcttggta
    14221 agagccagca aacaagtact tctagctcaa gttcaagtca aactagcagc tcaagttcaa
    14281 gtcaagcaag ttcaaatacg actagtgagc caaaaccaag tagttcaaat gagactagaa
    14341 gtagtcgcag tgaagtcaat atgggtctct cgagtgcagg ggttgctgtt caaagaagtg
    14401 ccagtcgtgt tgcctataat cagtctgcta ttgatgatag taataactct gcctgggatt
    14461 ttgcggatgg tgttttggaa caaattctag caacttcacg ttcacgtggc tatatcactg
    14521 gagaccaata tatccttgaa cgtgtcaata tcgttaacgg caatggttat tacaacctct
    14581 ataagccaga tggaacctat ctctttaccc ttaactgtaa gacaggctac tttgtcggaa
    14641 atggcgctgg tcatgcggat gacttggact actaagcagt cgttacaaaa ttctttcttt
    14701 tcaaaagtaa aaatgataaa ataaaacaaa tgaaacaaga ggagtgtcaa atgacaaaag
    14761 ctaactttgg tgtcgtaggt atggccgtaa tgggtcgtaa ccttgccctt aatattgaat
    14821 cacgtggtta cacagttgct atctacaacc gtagtaaaga aaaaacggaa gatgtgattg
    14881 cttgccatcc tgaaaagaat tttgtaccaa gctatgacgt tgaaagtttt gtaaactcaa
    14941 tcgaaaaacc tcgtcgtatc atgctgatgg ttcaagctgg acctggtaca gatgctacta
    15001 tccaagccct tcttccacac cttgacaaag gtgatatctt gattgacgga ggaaacactt
    15061 tctacaaaga taccatccgt cgtaatgaag aattggcaaa ctcaggtatc aactttatcg
    15121 gtactggagt ttctggtggt gaaaaaggtg cccttgaagg tccttctatc atgcctggtg
    15181 gacaaaaaga ggcctacgaa ttggtttcgg atgttcttga agaaatctca gctaaagcac
    15241 cagaagatgg caaaccatgt gtgacttata tcggtcctga tggagctggt cactatgtga
    15301 aaatggttca caacggtatc gagtacggtg atatgcaatt gatcgcagaa agctatgact
    15361 tgatgcaaca cttgctaggc ctttctgcag aggatatggc tgaaatcttt actgagtgga
    15421 acaagggtga attggatagc tacttgatcg aaatcacagc tgatatcttg agccgtaaag
    15481 acgatgaaga ccaagatgga ccaatcgtag actacatcct tgatgctgca ggtaacaagg
    15541 gaactggtaa atggacgagc caatcatctc ttgaccttgg tgtaccattg tcactgatta
    15601 ctgagtcagt gtttgcacgc tacatttcaa cttacaaaga agaacgtgta catgctagca
    15661 aggtgcttcc aaaaccagct gccttcaact ttgaaggaga caaggctgaa ttgattgaaa
    15721 aaatccgtca agccctttac ttctcaaaaa tcatttcata cgcacaagga tttgctcaat
    15781 tgcgtgtagc ctctaaagaa aacaactgga acttgccatt tgcagatatc gcatctatct
    15841 ggcgtgatgg ctgtatcatc cgttctcgtt tcttgcaaaa gattacagat gcttacaacc
    15901 gcgatgcaga tcttgccaac cttcttttgg acgagtactt cttggatgtt actgctaagt
    15961 atcaacaagc agtgcgtgat atcgtagctc ttgcggttca agctggtgtg ccagtgccaa
    16021 ctttctcagc agctattact tactttgata gctaccgttc agctgacctt ccggctaact
    16081 tgatccaagc acaacgtgac tactttggtg ctcacactta ccaacgtaaa gacaaagaag
    16141 gaaccttcca ctactcttgg tatgacgaaa aataagtagg tcagccatgg ggaaacggat
    16201 tttattactt gagaaagaac gaaatctagc tcatttttta agtttggaac tccagaaaga
    16261 gcagtatcgg gttgatctgg tagaggaggg gcaaaaagcc ctttccatgg ctcttcagac
    16321 agactatgat ttgattttat tgaacgttaa tctgggagat atgatggctc aggattttgc
    16381 agaaaaattg agtcgaacta aacctgcctc agtcatcatg attttagatc attgggaaga
    16441 cttgcaagaa gagctggaag ttgttcagcg ttttgcagtt tcatacatct ataagccagt
    16501 ctttatcgaa aatctggtag cgcgtatttc ggcgatcttc cgaggtcggg acttcattga
    16561 tcaacactgc agtctgatga aagttccaag gacctaccgc aatcttagga tagatgttga
    16621 acatcacacg gtttatcgtg gtgaagagat gattgctctg acccgccgtg agtatgacct
    16681 tttggcgaca cttatgggaa gcaagaaagt attgactcgt gagcaattgt tggaaagtgt
    16741 ttggaagtat gaaagtgcga ccgagacaaa tatcgtagat gtctatatcc gctatctacg
    16801 gagcaagctt gatgttaaag gacaaaaaag ctacattaaa accgtgcgtg gtgttggtta
    16861 caccatgcaa gaatagaaaa gcagttgcag ttgtgtaact gcttttttga ggagttttta
    16921 tatattgaca tgcagtcgtg tctttgctac aatcgtttta ggaggatata taaatgaaac
    16981 ttttgaaaaa aatgatgcaa gttctactag cagtcttttt ctttggtttg ctagctacaa
    17041 atacggtatt tgcgaatacc acaggtggcc gatttgttga taaggataat agaaaatatt
    17101 atgtaaaaga tgatcataaa gcaatctatt ggcataaaat agacggtaaa acttactatt
    17161 ttggtgatat tggagagatg gttgtcggtt ggcaatactt agaaattcct ggaacaggtt
    17221 atcgtgatga tttatttgat aatcgtccag tcttcgaaat tggccttcag gagaagtggt
    17281 actattttgg acaagatggt gctttgctag aacaaacaga taaacaagta ctagaggcaa
    17341 aaacgtctga aaatacagga aaagtatacg gtgaacaata tcctctatct gctgaaaaga
    17401 gaacttatta ttttgataat aattatgctg taaagacagg ctggatttat gaagacggca
    17461 attggtatta tttaaataag ctaggaaatt ttggtgatga ttcttacaat ccactaccaa
    17521 ttggtgaagt tgctaagggt tggactcaag attttcatgt tactattgac attgatagaa
    17581 gcaaacctgc tccatggtac tacctccgtt cctcaggagc aatggccact ggctggtatc
    17641 aggaaggtac cacttggtat tatttagacc agccaaatgg cgatatgaaa acaggttggc
    17701 aaaaccttgg gaacaaatgg tactatctcc gttcatcagg agctatggca actggttggt
    17761 atcaagatgg ttcaacttgg tactacctaa atgcaggtaa tggagacatg aagacaggtt
    17821 ggttccaggt caatggcaac tggtactatg cttatagctc aggtgctttg gcagtgaata
    17881 cgaccgtaga tggctattct gtcaactata atggcgaatg ggttcggtaa tgacagggcg
    17941 cttataatta tattagaaca ctcgcttaat caaagaagtg actttgttgt acttgacctg
    18001 acgattgggg agattaaaag catgtgaaat ctacacatgc tttttcgtta gttatgagtg
    18061 ttgaacttga ctaaccattg acaaaagata agagcaatca aaataagata caaaaatatc
    18121 aatgctaact gcaccgaaac gcccgatgct atggataggg ctaataggga gagcagggac
    18181 ggggcgagaa tgtctactgt gctcagagca gagcccaccg tccccatctt gtcactgggg
    18241 atatgggcaa agacagctgc ttgtaggcga gggctgagaa tgccgataat cgtagaactg
    18301 atgaaactgg tcacgaaaat tacgatatag gcttgacaaa ggatactagt tattagagcc
    18361 aatagggaaa tctcacagaa aaccaagagg tgcgtgaaac ggatattttt aaatagatta
    18421 ctgctggtca tattgccaag gatagctcca caagacatga tgatggagag cagggcgaag
    18481 agttgcccaa tgctcagttg tgctagtgtc tgttggatga agcggagggc aatcagccga
    18541 ggggacacat ccaagatggc aatcatactg gtcgtcagaa acagtagtat gacggtatct
    18601 tttaacctca ggagcacagc cagcgactgt ttaagatgtt gaaaatattt cttaaaactc
    18661 agtgctgtat gtgacatttc aatccttttt tcaacctcat acaaggtatg tcgaataaca
    18721 tacaggccca aaaaggcaat gacaaaagtc agagtgttga taagggaaat agtttgaata
    18781 cttataacat tgataaggaa tgcgccagcc agattggcga caatgtggac aaccctcatc
    18841 agagactgcc tgaaccccat ggcatcatgc aggtcatcct taattacact gatgtagagg
    18901 gcgttcatca tgtaggcaga caggtagctc aaggtgtcag agatgaggtt gatgatgaca
    18961 atcataatga ctgaaactaa tgaaaagggc gacaagacga gaaatactgt caaaatagcg
    19021 tagagcagga ttttgatttt ggcaatacag agtgcgtgtt tgactctatt ttcttgaaaa
    19081 tctcctagaa tcccgagaaa gagttgcgat agtaggggaa ccatctctga aatggaaatc
    19141 agtaaaatcg ctagcggagc gaaattgtac tgagccacat aattgataat ggcgaggtag
    19201 aaaatactgt cgccgaatga tgaaaaacag ctgtttagca gcaactgtct atatatgttg
    19261 tttgtccaaa ataatttcat acatttctcc ttatgtttgc acatcgaatg ggaatttatt
    19321 atttaataga caccaatata ccccctgctt cctattccga agtcaaacaa atctgcttta
    19381 ccactatctc cttgaaacat cagtaaaccg tgatcatctc gttcagaaca tgctaaaatt
    19441 acctgttgga tttttttcat cagtaatttg ttttgattgt agacagtggt ttgtagtaag
    19501 ctagttattc cgtggcaata tcctgtcatc atttttagat gatggttaaa aacaaattct
    19561 tgatatttac taataatatc tttgtttccg tcacaatcgt acatacaaag atataagatt
    19621 attccggaaa ttccttcgca ccaagaaagt tgtaaatttc ctaaattttc agtattagaa
    19681 gtaacttttt ctaataattt ttttaattca gcatggaatg tatggagatc attatagaac
    19741 atagaaggtt ctaagacttt agaataggca aataaagcat atgcaatttc acaatatcca
    19801 tgagcaaagc tatagtctaa aatgctttct ttggctgtat ctctttgtag gaaataatga
    19861 taaatttccc catatactat tgtggatgaa agttttaaga gtattgtcat tggtaaattg
    19921 atagtatttt attaatgata ataggacacc agattttccc aatgcaaagt ctatttcttc
    19981 tagagtatca taattctcta ttaatttctt agcggtgacg tttgctaatt gtagatagta
    20041 ttgattttta ctgatagaaa ataaatgtag gagtaaccat agatagcctg catcaccaaa
    20101 tagcagtgag tattgatatt catatgtgtg tcgttctaat tgtcactgcc ttcttgatga
    20161 tagagagtat tttagaatcc agttctgggg ggtacaattt atttagagga aacagtagtc
    20221 cagctattcc gtgttggaat gataaattac ttacgaaatt tccaaattct gttgaagaag
    20281 tgatccttcc ttcagaattt acagttttat ctagtaaaaa tcgtagaaca ctattacatt
    20341 ctgttttgta atcatattta tgttcctcta tttttttaat tttgactaaa ttatttgttt
    20401 gacttaaatt tagtagacat tgtaaccaaa atagttgctc atctgtgagc tgaccttgag
    20461 tatgtgatag ttctagtaat tgaataggat cctttagtta gattcaatca atctataaca
    20521 caacaacata ataatacttt taggataact tgtcaaatat tatagtaaaa taaaataa
//
LOCUS       DAVBCN010000032        20575 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVBCN010000032 DAVBCN010000000
VERSION     DAVBCN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20575
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.032"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..128
                     /locus_tag="VQ497_001095"
     CDS             <1..128
                     /locus_tag="VQ497_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2517410.1"
                     /translation="FRSLENLEQAIVDYIDYYNNKRIKVKLKELSPVQYRTKSFG"
     gene            complement(176..2719)
                     /locus_tag="VQ497_001096"
     CDS             complement(176..2719)
                     /locus_tag="VQ497_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HET2517411.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQKKVD
                     ILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTKTFT
                     EAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQAQT
                     GFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(2781..4403)
                     /locus_tag="VQ497_001097"
     CDS             complement(2781..4403)
                     /locus_tag="VQ497_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2517412.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            4609..5634
                     /gene="trpS"
                     /locus_tag="VQ497_001098"
     CDS             4609..5634
                     /gene="trpS"
                     /locus_tag="VQ497_001098"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET2517413.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            5787..7265
                     /gene="guaB"
                     /locus_tag="VQ497_001099"
     CDS             5787..7265
                     /gene="guaB"
                     /locus_tag="VQ497_001099"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET2517414.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(7316..8413)
                     /gene="recF"
                     /locus_tag="VQ497_001100"
     CDS             complement(7316..8413)
                     /gene="recF"
                     /locus_tag="VQ497_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HET2517415.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(8416..8784)
                     /locus_tag="VQ497_001101"
     CDS             complement(8416..8784)
                     /locus_tag="VQ497_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET2517416.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SCRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            8943..10193
                     /locus_tag="VQ497_001102"
     CDS             8943..10193
                     /locus_tag="VQ497_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET2517417.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPAVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKGWIAKLEQV
                     DKDAICRAANNVKLQAIYFMEGIE"
     gene            10190..11473
                     /locus_tag="VQ497_001103"
     CDS             10190..11473
                     /locus_tag="VQ497_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET2517418.1"
                     /translation="MTKVVFEKKYYPAVKEMIYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            11515..12336
                     /locus_tag="VQ497_001104"
     CDS             11515..12336
                     /locus_tag="VQ497_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2517419.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            12347..12892
                     /gene="pgsA"
                     /locus_tag="VQ497_001105"
     CDS             12347..12892
                     /gene="pgsA"
                     /locus_tag="VQ497_001105"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET2517420.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIILIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            12889..13716
                     /locus_tag="VQ497_001106"
     CDS             12889..13716
                     /locus_tag="VQ497_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET2517421.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            13701..14540
                     /locus_tag="VQ497_001107"
     CDS             13701..14540
                     /locus_tag="VQ497_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET2517422.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            14533..15327
                     /locus_tag="VQ497_001108"
     CDS             14533..15327
                     /locus_tag="VQ497_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2517423.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            15386..16204
                     /gene="mreC"
                     /locus_tag="VQ497_001109"
     CDS             15386..16204
                     /gene="mreC"
                     /locus_tag="VQ497_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET2517424.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTVVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            16204..16698
                     /gene="mreD"
                     /locus_tag="VQ497_001110"
     CDS             16204..16698
                     /gene="mreD"
                     /locus_tag="VQ497_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET2517425.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            16792..17970
                     /locus_tag="VQ497_001111"
     CDS             16792..17970
                     /locus_tag="VQ497_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HET2517426.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            18194..18973
                     /gene="rpsB"
                     /locus_tag="VQ497_001112"
     CDS             18194..18973
                     /gene="rpsB"
                     /locus_tag="VQ497_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET2517427.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            19051..20091
                     /locus_tag="VQ497_001113"
     CDS             19051..20091
                     /locus_tag="VQ497_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HET2517428.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            20337..20575
                     /gene="tnpB"
                     /locus_tag="VQ497_001114"
                     /pseudo
     CDS             20337..20575
                     /gene="tnpB"
                     /locus_tag="VQ497_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT         6546 a         3290 c         4257 g         6482 t
ORIGIN      
        1 gctttagatc tttagaaaac cttgaacaag ctattgtgga ctacattgat tactacaaca
       61 acaaacgaat taaggtaaaa ctaaaagaac ttagccctgt gcaatacaga actaaatcct
      121 tcggataaat taattgtcta actttttggg gtcagtacat taactggtcg attttttatg
      181 ccttagatga ctttcgtctg tgattataga ttccaaatag taagagagaa gtaaaggaac
      241 agattgctcc agtaataaaa ccattgggaa tgaaggaaag tgtaatagtt cctttcccct
      301 tgggaatgtc aactttcata aatccagttt gagcttgttt aatttctatt ttcttaccat
      361 cttggtaggc agaccaacct ttgtcataag gaatggtgaa gaaaatagat gtatcttgtt
      421 ggacatcata tgtagcaaaa accttgtttt tagaagttga tactgtgaca ggttgttctt
      481 taattttttg aattgcctcg gtgaaagttt tggtatctaa acgatagaag gtaggagatt
      541 caaatgatac ttgtgaattt ccagggaaac taacattgat attgaaagtt tttttctctt
      601 tagtatatcc tagattaaag aaggagaaga cattatcagt tgtaaaagtc tttttttcac
      661 catttacaag gatgtcaacc ttcttttgtt tatcgttaga aaagtgaagg tttatgaaag
      721 agagataaac ttggctgttt tctggaactt caatttgata ctggattgct gcatcttcat
      781 ttgaagaact tgtgacacta atcaaatcat tagtattttc tgttttttca taaggtattg
      841 gagaaaaata atcaaaattg acgttagcaa gttgatttaa aaatgaggcc tgattatcca
      901 aggtatgttc attgaacttg acatcattgt aaacagattg actagcaact gcaatcggaa
      961 gagagtattg attttcatat agggtaagat tatctttttg atagatatct ttaaagccat
     1021 acttatcaat aggactgtct gagatattgt actggatacc aaataaacta tcagccaaaa
     1081 tactattatt tgcatatcgg agattgagat tagtcccaga ggatttaaaa ccaagtttat
     1141 ctaaagtaga gcttgatgaa cgatttcgaa cagatgaaaa ttgagagatt ccattgtagt
     1201 tgaatttcat actgtcattt cctgtctgag tttgtagttt ttcagtacga gtaaattgat
     1261 ttccaatata tgttgagaaa gattccatag ctgggatatc tcgactataa gcacttcgag
     1321 aagcaaatcc ccattcctta gcaattccgt ccatttgaga tgaagcattt aaactcattt
     1381 caaccagtat aaataaagag attagaatgg caaatagatt cacagatata aactttttga
     1441 taactgcaag gagtaaaaga gaatagacaa ccaaaaattc aagagtaagc agaatattca
     1501 aatctgttaa aaaagaataa tgcgatttta gatagatggt agctaaaaat cctgctacta
     1561 caagaaaaag cgaaactaaa aaattccaga ctttaagttc tttcagacgc tttaagactt
     1621 ctgctgctgt gtaaattaac aaggtagaga aaatccaagc atagcgatgt aaaaacatgt
     1681 ttggagtatg catgccttgc caaaataagt caagagcttc tatgtaaaag cttgcaatta
     1741 gaaatgcaaa gaatattaca tatatgagtt tcacgtgaaa cttaatagat ttcagcgtaa
     1801 aaaataaaat ggtcaaaata aagggaaata gtccaacaaa aatcattggg atggccccat
     1861 actttgttgt gtcaaaggaa ccaatgaatt gcttagcaaa gagatcaaga taccagctac
     1921 tttcagtttg aaactttgta acttcagtca atttttcccc atgtgtctgt aaatcaaata
     1981 gagtgggaag agtcataatc aaactagcca taccagctaa aaaggagata actatgaaat
     2041 caagaacaga tgattttcga gtcttaaagt cccacgaaat ttgacagaga taccagaaaa
     2101 taagaaacaa tactgtcata tatccaaaat aataattttg aataaataag attgacagac
     2161 ttgtaaagta caataggagt ttcttttcag ttatcagtag atgtaaacca gttataatta
     2221 aaggaatcaa gataaaaaca tctagccagg tttttatctc taattgactg acagtgaaac
     2281 tcatcagagc ataggaagta gataaggcta gttttaaagt ctgagggata gattgaaaca
     2341 atttattcaa actaaaaaag gttgacagac caatcaatcc aaattttaag agagtggtca
     2401 gatagacagc atctggcata ttcgttagat caaaaaagta aactagaggc gcgagaaaac
     2461 tacccaagta ataactagat agggcataga agtttagccc tagaccactt gtaaaggtgt
     2521 aaaacagact actatttcca tgtaggatat ttcgtaaggc tacatcaaaa ataacgtatt
     2581 gatgaaagcc atctcctaat agaggagagt tgtcgctatt ccagtagata ccttgagata
     2641 gatatactcc agacataatc actacaggaa tgatgaaaga aataaaatag gttcgatatg
     2701 tttttaaaaa tgatttcatg ttacctcgta gaatgataga aaactcagtt ggttaaccca
     2761 actgagtttt gaagttttat ttagtctttc caaagttctt taacttttgc ttgtacttct
     2821 gcattttcta ggaattcatc gtaggtttca tcgatacggt caatgacgcc atttttagat
     2881 aagacaatga tatggttagc caaagtttga ataaactcgt ggtcatggct ggcaaagatg
     2941 attgattctt taaagttttt cagtccatca ttcaagcttg agatagattc caagtccaag
     3001 tgatttgttg gatcatcaag tacaaggaca tttgatttta agagcatgag ttttgaaagc
     3061 atgacacgaa ctttttctcc ccctgacaag acatttacag gtttgttaac ttcatctcca
     3121 gagaagagca tacggccgag gaaaccacgt aggaaagtat tgtcatcttc ttctttactt
     3181 gcgaattgac gcaaccagtc aagaattgat tctcctcctg caaaatcagc tgagttatct
     3241 tttggtaggt aagattgact agttgtaact ccccacttga cagttccttc atagtcaata
     3301 tctcccatga ttgcacgaat taatgcagtc gtttgaatat cattttgtcc aataagtgct
     3361 gtcttatcat ctggacgcaa gatgaaacta atattatcca agatagtttc accatcaatc
     3421 tttacagtta gattttctac tgtcaagaga tcattaccaa tctcacgttc cgctttaaag
     3481 ttgataaatg gatatttacg actagatggt acaatctctt ctagctcaat cttatcaagc
     3541 attttcttac gtgatgttgc ctgccttgac ttagaagcat tggcagagaa acgagcaacg
     3601 aattcttgca attgtttaat tttttcttct gctttagcat tacggtctgc tagcaattta
     3661 gcagcaagct cagaagattc cttccagaag tcgtagtttc cgacatagag tttgattttt
     3721 ccaaagtcaa ggtcggccat gtgagtacaa actttgttta agaagtgacg gtcgtgggat
     3781 actacgataa ctgtgttatc aaagtcaatc aagaagtctt ctaaccaagt aatcgattgg
     3841 atatccaaac cgttagtagg ctcgtccaag agaagaacat ctggtttacc aaaaagtgct
     3901 ttggcgagga gaacctttac tttttcaccg ttggccaatt cgctcatgtt ttggtagtgt
     3961 aattcttctg gaatgtttag gttttgaagt agttgagagg cttcactctc tgcttcccaa
     4021 cctccaagtt cggcaaactc tccttcgagt tcggcagcac gaaccccgtc ctcgtctgag
     4081 aaatcttcct tcatgtagat agcatctttc tctttcatga tgctataaag tttttcattt
     4141 cccatgataa cgacatcaat ggcacgttca tcttcgtagt caaagtgatt ttgacgaaga
     4201 acagagagac gttcatctgg accaagagag atgtgaccag tagtaggttc gatatctcca
     4261 gctaaaattt ttaaaaaggt tgattttccg gcaccattag caccgattaa tccgtaagta
     4321 tttccttctg taaatttgat attgacatca tcaaaaagtt tgcgatcact aaaacgtagt
     4381 gaaacatcag atactgtaag caatgttttt ctcctatatg tgtaatatat ttattctact
     4441 agaaaataca gaaatattca aatttttatt tgtcaatttt gtgtaaatta tatttacagt
     4501 atcctttaca caaatctgta aaaagcaagg ctgatttatt ttgataaatt acggttattt
     4561 cattaaaaaa atgctataat tgaaaggact atatcgaagg agaacaaaat gactaaaccc
     4621 attattttaa caggagaccg tccaacagga aaattgcata ttggacatta tgttggaagt
     4681 ctcaaaaatc gagtattatt acaggaagag gataagtatg atatgtttgt gttcttggct
     4741 gaccaacaag ccttgacaga tcatgccaaa gatcctcaaa ccattgtaga gtctatcgga
     4801 aatgtggctt tggattatct tgcagttgga ttggatccaa ataagtcaac tatttttatt
     4861 caaagccaga ttccagagtt ggctgagttg tctatgtatt atatgaatct agtttcgtta
     4921 gcacgtttgg agcgaaatcc aacagtcaag acagagattt ctcagaaagg atttggagaa
     4981 agcattccga caggattctt ggtctatcca atcgctcaag cagctgatat cacagctttc
     5041 aaggctaatt atgttcctgt tgggacagat cagaaaccaa tgattgagca aactcgtgaa
     5101 attgttcgtt cttttaacaa tgcatataac tgtgatgtct tggtagagcc ggaaggtatt
     5161 tatccagaaa atgagagagc agggcgtttg cctggtttag atggaaatgc taaaatgtct
     5221 aaatcactaa ataatggtat ttatttagct gatgatgcgg atactttgcg taaaaaagta
     5281 atgagtatgt atacagatcc agatcatatc cgcgttgagg atccaggtaa gattgaggga
     5341 aatatggttt tccattatct agatgttttt ggtcgtccag aagatgctca agaaattgct
     5401 gatatgaaag aacgttatca acgaggtggt cttggtgatg tgaagaccaa gcgttatcta
     5461 cttgaaatat tagaacgtga actgggtcct attcgtgagc gccgtattga atttgctaag
     5521 gatatgggag aagtttataa tatgattcaa aaaggtagtg aaagagcgcg tgaagttgcg
     5581 ggtcaaaccc tatctgaggt aaaaggggca atgggacttc attactttaa ctaagtttat
     5641 tttacaagaa actatcattt ctatctcgaa gaaaagatag tttttttatt tacccctgta
     5701 acatttgaca aatttttata gaatggtatg atagatacaa tataaaaaga gtcaagctca
     5761 aaaagaaaga aaagaggaaa cttcgaatgt ctaattggga cactaaattt ttgaaaaaag
     5821 gttttacctt tgatgatgta ttgcttattc cagctgaaag tcatgtgttg cctaacgatg
     5881 cagatttaac aactaaattg gcagataatt tgactttaaa tatcccaatt attaccgctg
     5941 ccatggacac agttacagag agtcaaatgg ccattgctat tgctcgtgca ggcggtctcg
     6001 gagttatcca taaaaacatg tcaattgctc aacaagcaga cgaggttcgt aaggtaaaac
     6061 gttctgaaaa tggagttatt attgatccgt tcttcttgac gcctgaacat acaattgctg
     6121 aagcagatga gcttatgggt cgttaccgca tcagtggtgt tccagttgtt gaaacacttg
     6181 aaaatcgtaa attggttggt attttgacaa accgagatct tcgttttatt tcagattata
     6241 atcaaccaat ttcaaaccat atgactagtg aaaatcttgt tactgctcct gtgggtacgg
     6301 atcttgcaac ggctgagagt attcttcaag agcatcgtat tgaaaaactt ccgttggtcg
     6361 atgaagaagg cagtctttct ggtttgatca ctatcaaaga tattgaaaaa gttattgagt
     6421 ttccaaatgc ggctaaagat gagtttggtc gtcttctagt tgcaggtgca gtaggtgtta
     6481 cttcagatac atttgaacgt gcagaggctc tttttgaggc aggagcggat gcgattgtta
     6541 ttgatactgc acatggtcat tctgcaggtg tcttgcgtaa aattgccgag attcgtgctc
     6601 atttcccaga tcggactttg attgctggaa atattgctac tgctgaaggt gcacgtgccc
     6661 tttatgaagc gggtgtagac gttgttaagg ttggtattgg accaggttct atctgtacta
     6721 ctcgtgtgat tgctggtgtt ggtgttccgc aagtaacagc tatctacgat gctgcagctg
     6781 ttgcgcgcga atatggtaaa acgattattg ctgacggtgg gatcaagtat tctggagata
     6841 ttgtaaaagc acttgctgca ggtggaaatg ctgttatgct tggatctatg tttgctggaa
     6901 ctgatgaagc tccaggcgaa actgaaatct tccaaggacg taaattcaag acttaccgtg
     6961 gtatgggatc aattgctgct atgaagaaag gttcaagcga ccgttatttc caaggttctg
     7021 tcaatgaagc aaacaagctt gttccagaag gaattgaagg tcgtgttgct tataaaggag
     7081 cggcagctga tattgttttc caaatgattg gtggtattcg ctctggtatg ggttactgtg
     7141 gtgcagctaa ccttaaagaa ctacacgata atgctcaatt tattgaaatg tctggtgctg
     7201 gtttgaaaga aagccatcct catgatgtgc aaattactaa tgaggcacca aattattcta
     7261 tgtaaaaaac aatgaaaaga actccagtga aaacaggagt tcttttacaa tgttgtcaat
     7321 ttccatttac agcagcttta ccatcctgaa tagtgaagat acttagattt tctggcagat
     7381 tttgaagatg gtctaagctt gttgttgtga taaaggtttg gattgattga gaaatcgttt
     7441 ctaataattt taactgtcta gtgttgtcaa gttcactcat cacatcgtca agcagtaata
     7501 taggagattc tgtggtaatg ctttccatta attcgatttc tgctaatttt atcgagagga
     7561 cgagactacg atgttgacct tggcttccga aactagcatc catcccattt atataaaaag
     7621 aaatgtcatc tcgatgagga ccgacaccag tattcttttt aaataaatct ctggatctac
     7681 ttttttctaa agcaattttg aaagattcgg ataagttttg tttgtcagtt atattgacag
     7741 aagattgata ggatattgac aactcttcga tctgattaga gagttcaaaa tgtttcttac
     7801 gcccaaatga ttctagtttt tttatgaaat ctaagcggtg attcattaca cgacatccat
     7861 aatcaactag ctgatcatct aacacagaaa ggaatgtttc atctattttt tgagctgatt
     7921 ttaggtaagt gtttctttgc tttaggatgt ggttataatt ggttaagtca gataaataga
     7981 ttggcttaat ttgcccaagt tccatatcaa tgaattttcg tcgaatcgaa ggtgctcctt
     8041 taattagttg taaatcttca ggagcaaata agacaacatt catgtgtcct acataatctg
     8101 aaaggcgtgc ctgttttaag tgattaactt ttgttacacg gcctttttgt gttagttcga
     8161 tttctagggg aatggatcca gtttttttct gaacgagacc tgaaagatga agttgttcct
     8221 catcaaaatg aatgagattt ttatctgttc gagttcgatg actacgcgtt aaggctaaaa
     8281 aatagatagc ctctaacatg tttgtttttc cttgtgcatt acgtcccaaa aagacattca
     8341 atttaggatt aaagtctatt ttcgtttctt tgtaattacg aaaagtcttg agagatagat
     8401 gttgtagcca catgattatc taccagggaa tcgtggagct tgttggcttt taggtgatga
     8461 agcaggtttc gatttgtctt ttttaactcc cttattcatc tttttgacaa gtttagcgat
     8521 ccgttctttt tcaactttat cagcttggta ttcatcttgt tcttcagaag taggttgtgt
     8581 caacaagatg tcaatgttca tgtcagggat gtcaacttta tcgccaatac gaagtttttt
     8641 tccacgacaa ctctctaatt ccctattaaa gtaaacagaa tgttcagaga gaaatgattt
     8701 aatagctcct ccgctatgtg taattccaag ttctttgagt agtgcttgga gggtaataaa
     8761 ttcttcaaat aatttgtatt ccataattca cctcaatctt tggtattata ccatattttc
     8821 ctaaaaatag tgagagagac agctagaaat gtaagcgatt tttatttcaa aagtgttaca
     8881 gggaacaaga aaatttcagg ttttcgtgat ataatagaag tctgtatata aggaggtaaa
     8941 tcatggagtt agtgcatgga atttcaacac attttatcca atcaaaaaag tttaaaacaa
     9001 acaaaattac cgtgcgtttt accgctccat tatcccttga tacgattgca ggtcacatgt
     9061 tgagtgcaag tatgctagag actgctaatc agatgtaccc cacttctcaa gatttgagga
     9121 gacacttggc cagtctatac ggtacagata tgtcaaccaa ttgtttcaga agagggcaaa
     9181 gccacattat agaattgaca tttacctatg ttcgtgatga gtttttaagt aggaaaaacg
     9241 tgctaacctc tcagattttg gaacttgtaa aagaaactct tttttcaccc gcagtagttg
     9301 ataatgggtt tgatccggcc ttatttgaaa ttgagaaaaa acaattgcta gcaagtttag
     9361 cagctgatat ggatgattct ttttattttg cacataaaga attggataaa ttgttttttc
     9421 atgatgaacg tcttcaattg gaatatagtg atttacgaaa tcgtatttta gctgaaactc
     9481 cacaaagttc ttattcttgt ttccaagaat ttttagccaa tgatcgaata gatttctttt
     9541 tcctaggtga ttttaatgag gttgaaattc aaaatgtatt agaatcattt ggctttaaag
     9601 gtcgaaaagg agatgtgaag gttcagtatt gtcaacctta ttctaatatc cttcaggaag
     9661 gtatggttcg gaaaaatgtg ggacaatcca ttttggaatt aggttatcat taccgttcta
     9721 aatatggtga tgaacaacat ttacccatga ttgtaatgaa tggtttactt ggtggatttg
     9781 ctcactctaa gctctttaca aatgtccgtg aaaatgctgg attagcttat accatttcaa
     9841 gtgagcttga tttatttagt ggattcttga ggatgtatgc tggtatcaat cgagaaaatc
     9901 gtaaccaggc tcgtaaaatg atgaataatc aactgcttga tttaaaaaaa ggttatttta
     9961 cagagtttga gttaaatcag accaaggaaa tgattcgttg gtcgttgtta ctttctcaag
    10021 ataatcaatc ttcattgatt gaacgtgctt atcaaaatgc cttatttgga aaatcttcag
    10081 cagactttaa aggttggatt gcaaaactcg agcaagttga caaagatgct atttgtagag
    10141 cagctaataa tgtgaaacta caggcgattt actttatgga aggaatagaa tgacaaaggt
    10201 tgtttttgaa aaaaaatact atccagctgt aaaagaaatg atttatcgaa ctcgtttggc
    10261 caacggattg acagttgctc ttttgcctaa aaaggaattt aaagaggttt acgggagtgt
    10321 cactgtacag tttggttcgg tagatacgtt tgtcacagaa gttgacggag atgtaaaaca
    10381 atatcctgga ggaattgctc attttcttga acataaatta tttgagagag aagattctag
    10441 tgatttgatg tcggctttta cgagtctagg tgcagatagt aatgccttta caagctttac
    10501 aaaaacaaac tatctttttt cagcaacgga ttatttttta gaaaatttag atttacttga
    10561 tgaattggta acatcagcac actttactga agcttccatt ctgacagagc aggatattat
    10621 tcagcaagaa cgagaaatgt accaagatga tccagattcg tgtttattct tttcaacttt
    10681 agcgaatttg tatcctggta cacctttagc aactgatata gttggaagtg aggagtccat
    10741 ttcccaaatc aatctaacta atttgcaaga aaattttaca aagttttaca aacctgtaaa
    10801 catgtctctg tttttagttg gtaattttga tgtggagcga gtacaggact attttgaaag
    10861 caaagaactg aaagattcag attttcagga agtagcaaga gaaaagttgt ttttacagcc
    10921 tgtaaagcca acagatagta tgagaatgga agtatcttct cccaaactag cgattggagt
    10981 tagaggtaag cgagaagttt ctgaagcgga ttgctatcga catcatattt tattaaaatt
    11041 attgtttgca atgatgtttg gttggacttc ggatcgtttt caaaaatgtt atgaatcagg
    11101 taaaattgat gcgtccttat ctctggaagt tgaaataaca agtcgctttc attttgtcat
    11161 gttgacaatg gatacgaaag agccagttgc tttgtctcat caatttagga aggctattcg
    11221 taattttaca aaggatttag atattacaga ggaacattta gatattatca aaagagagat
    11281 gtttggcgaa tttttcagta gcatgaactc tcttgaattt attgcaacgc aatatgatgc
    11341 ttttgaaaat ggtgagacaa tttttgattt gccgaaaatt ttacaggaaa ttactttaga
    11401 ggatgtcctt gatgctggac atcatttaat agatgatggt gacatagttg attttacaat
    11461 atttccatcg tagtaaccta tcataataga cactagaaag aagggatgac aagtatgaga
    11521 aaaaaaacaa ttggagaggt tttacgatta gctagaatca atcagggatt gagtttagat
    11581 gaattgcaga aaaagacaga aatccagtta gatatgttgg aagcaatgga agcagacgat
    11641 ttcgatcaac ttccaagtcc tttttacacg cgttctttct tgaaaaaata tgcatgggct
    11701 gttgagttag atgaccaaat tgttttggat gcttatgatt ctgggagtat gattacttat
    11761 gaggaagtag atgttgatga agatgagttg acaggtcgta gacgttcaag taagaaaaag
    11821 aagaaaaaaa catcattttt acctttattt tattttatcc tttttgcttt atcgatttta
    11881 atttttgtga cttattatgt ttggaactat attcaaactc aaccagagga gccttctctt
    11941 tctaattaca gtgtggttca atcaacaagt tcaactagct ctgttcccca ctcctcaagt
    12001 agtagttctt ctagtataga atcagctata agtgtatcag gcgaaggaaa tcatgtagaa
    12061 atcgcttata agacaagtaa ggaaacagtt aaattgcaat tggcagtttc agatgttaca
    12121 agttgggtca gtgtttcaga aagcgaactt gagggcggtg taaccttatc gccaaagaag
    12181 aaaagtgcag aagcaacagt tgcaactaaa agtcctgtaa caattacgtt aggtgttgta
    12241 aaaggtgttg atttgacagt agataatcag actgttgatt tatcgaaatt aacagctcag
    12301 actggacaaa tcactgtaac ctttactaaa aattaaggaa aaacgaatga aaaaagaaca
    12361 aattcccaat ctcttaacaa taggtcgaat tctctttata cctattttta tctttatttt
    12421 aacgatagga aattcgatag agagtcatat agttgcagct attatctttg ctgttgccag
    12481 tattaccgac tatttagatg gatatttagc tcgtaaatgg aatgtggtca gtaattttgg
    12541 taaatttgca gatcctatgg cggataagtt actagttatg tcggctttta ttatcttgat
    12601 tgagttaggt atggctccgg cttggattgt tgcagtgatt atctgtcgtg agttagctgt
    12661 gacaggttta aggcttttat tggttgaaac tggtggaaca attttagcag cagcaatgcc
    12721 tggaaaaatt aaaactttta gtcagatgtt tgccattatt ttcttgctat tacattggac
    12781 tttgcttggt caagttctac tttatgtagc cttatttttc actatctact ctggctatga
    12841 ctatttcaag ggtagtgcct atgtatttaa agggacattt ggttcgaaat gaaatcaata
    12901 attgatgtaa aaaatctttc ttttcgttat aaagaaaatc agaactacta cgatgtgaag
    12961 gatattacgt ttcacgtgaa acgtggagaa tggctttcga ttgtagggca taatggtagt
    13021 ggtaaatcaa cgacggttcg attaattgat ggcttactgg aagcagaatc tggagagatt
    13081 gtaattgatg gccaacgttt gactgaggaa aatgtttgga atatacgtcg tcaaatcggt
    13141 atggtttttc aaaatccaga caatcaattt gttggagcga ctgttgaaga tgatgttgct
    13201 tttggtttgg aaaatcaggg actttctcgt caagaaatga aaaagagagt ggaagaagct
    13261 ctggctttag ttggcatgtt ggactttaaa aagagagagc cagcgcgtct atcaggtggc
    13321 caaaagcaac gtgtggccat tgcaggtgtt gtagccctaa gaccagctat tttaatctta
    13381 gatgaagcaa cgagtatgtt ggatcctgag gggcgtagag aacttattgg gacagtaaaa
    13441 ggaattcgaa aagactatga tatgacggtc atttctatta cccatgattt ggaagaagtc
    13501 gccatgagtg atcgcgtatt ggtcatgaaa aaaggggaaa ttgaatcaac tagtagtcca
    13561 agggagcttt tctctcgaaa tgatttagat caaattggat tagacgatcc ttttgctaat
    13621 caattaaaaa aatctttgag ccagaatggc tatgatttac ctgaaaatta tttgacagaa
    13681 agtgagctag aggataagct atgggaattg ctctagaaaa tgtgaatttt acatatcaag
    13741 aaggtactcc cttagcttca gcagctttgt cggatgtttc tttgacgatt gaagatggct
    13801 cttatacagc tttaattggg cacacaggta gtggtaaatc aactatttta caactcttaa
    13861 atggtttatt ggtgccaagt caagggagtg tgagggtttt tgatacctta atcacctcga
    13921 cttctaaaaa taaagatatt cgtcaaatta gaaaacaggt tggcttggta tttcagtttg
    13981 ctgaaaatca gatttttgaa gaaacggttt tgaaggacgt tgcttttgga ccgcaaaatt
    14041 ttggagtttc tgaagaagat gctgtgaaga ctgcgcgtga gaaactggct ctggttggaa
    14101 ttgatgaatc actttttgat cgtagtccgt ttgagctgtc agggggacaa atgagacgtg
    14161 ttgccattgc aggcatactt gccatggagc cagctatatt agtcttagat gagccaacag
    14221 ctggtctaga tcctctaggg agaaaagagt tgatgaccct gttcaaaaaa ctccaccagt
    14281 cagggatgac catcgtcttg gtaacgcatt tgatggatga tgttgctgaa tatgcaaatc
    14341 aagtctatgt aatggaaaag ggacgtttag taaagggggg caaaccaagt gatgtctttc
    14401 aagacgttgt ttttatggaa gaagttcagt tgggagtacc taaaattacg gccttttgta
    14461 aacgattggc tgatagaggc gtgtcattta aacgattacc gattaagata gaggagttca
    14521 aggagtcgct aaatggatag tatgattttg gggcgttata tcccagggga ttcgattgtt
    14581 caccgattgg atccacgtag caaattgctt gctatgatgc tactgatttt gattgtgttt
    14641 tgggctaata accccttgac gaatctgatt ctttttatag cgacagggat atttattgcc
    14701 ttgtcaggag tatctctttc attttttatt cagggcttga agtctatgtt tttcttgatt
    14761 gccttcacaa ctatttttca actatttttc atttctaatg ggaatgtttt atttgagttt
    14821 tcgtttgtga gaatcacgga ttatgctttg caacaagctg ggattatttt ttgtcgcttt
    14881 gtattgatta ttttcttttc aactttgtta accttaacaa ccatgccttt aagtttggca
    14941 tcagctgtcg aagctttatt agcaccttta aagcgtgtga aagttccagt tcatgaaatt
    15001 ggattgatgc tgtctatgag tttgcgtttt gtcccaacct tgatggatga tacgactcgg
    15061 attatgaatg cacagaaagc tcgtggagtg gattttggag aaggaagcat cgttcaaaaa
    15121 gtaaaggcga tgattcccat tttgattcct ctttttgcga caagtttaaa acgtgcagat
    15181 tccttggcta tcgctatgga agcgcgtggc tatcagggtg gaaaaggcag aagtcaatac
    15241 cgacaattga aatggactct aaaggatacg ctgaccattc ttgttattct cgtacttggt
    15301 tgttgtttat ttttcttaaa atcttagtag ctttcaggaa ttgataaaaa gttactgtaa
    15361 cagtttttga tataaaggta ggaatatgaa ccgttttaaa aaatcaaaat atgtcattat
    15421 tgtttttgtc actgttctgc ttgtgtcagc tctcttagcg acgacttatt caagtacagt
    15481 tgtgacaaaa ttaggagatg gaatctcatt ggttgataga gttgtacaaa aaccttttca
    15541 gtggtttgat tctgtcaaat cagatttggc tcatttgaca cgaacatata atgaaaatga
    15601 aagtttgaag aaacagcttt accaattaga agttaaatca aatgaggtgg aaagtttaaa
    15661 gacagaaaat gaacaactgc gccaattgct tgatatgaag tctaaattgc aagccacaaa
    15721 gactttagca gcagatgtta ttatgcgttc tccggtatct tggaagcagg agttgacctt
    15781 agatgcaggt agatcaaaag gtgcttctga gaacatgtta gctattgcaa atggtggctt
    15841 gattgggagt gtttcaaaag tagaggagaa ctctactata gtcaaccttc tgacaaatac
    15901 ggaaaatgct gataagattt ctgttaaaat tcaacatggc tctactacaa tttatggaat
    15961 tattattggc tatgacaagg aaaatgacgt tcttaaaatt agccaattaa atagtaatag
    16021 cgatattagt gcgggagata aggtgactac tggtggatta ggaaacttta acgttgctga
    16081 tattcctgtt ggtgaagtgg ttgccacaac gcatagtaca gactatttga cacgagaagt
    16141 aactgttaaa ttgagtgcag atactcataa tgtagatgtg atagaattag tggggaattc
    16201 ataatgagac agttgaagcg agttggagta tttttattgc ttcctttctt tgttctaatt
    16261 gacgcccata ttagccagct tctgggctca tttttccccc atgtacattt ggctagtcat
    16321 tttctttttc tatttctctt atttgagacg atagaagtat cagagtatct ctacctagtc
    16381 tattgttttg ttataggctt ggtttatgat gtttactttt tccatctaat agggattaca
    16441 actctcttat ttatcttatt gggagccttc cttcataaat tgaatagtgt tattttgttg
    16501 aatcgttgga caagaatgct agctatgatt gtgctgacat tcctgtttga aatgggtagt
    16561 tatcttttgg cttttatggt agggttgaca gtagatagca tgtcgatttt tatagtctat
    16621 agcttggtac cgacgatgat tttaaatttt ttatggatta ctgtttttca atttattttt
    16681 gaaaaatatt atctataaga acgacatata aatgtaacaa aggcgtaata tttattaggc
    16741 ctttttttgg tatactagta ttgtctttaa aagaaggagt atctacgtaa tatgaagaaa
    16801 aaaatcttag cgtcactttt attaagtaca gtaatggttt ctcaagtagc tgttttaaca
    16861 actgcgcatg cagaaacgac tgatgacaaa attgctgctc aagataataa aattagtaac
    16921 ttaacagcac aacaacaaga agcccaaaaa caagttgacc aaattcagga gcaagtatca
    16981 gctattcaag ctgagcagtc taacttgcaa gctgaaaatg atagattaca agcagaatct
    17041 aagaaactcg agggtgagat tacagaactt tctaaaaaca ttgtttctcg taaccaatcg
    17101 ttggaaaaac aagctcgtag tgctcaaaca aatggagccg taactagcta tatcaatacc
    17161 attgtaaact caaaatcaat tacagaagct atttcacgtg ttgctgcaat gagtgaaatc
    17221 gtatctgcaa acaacaaaat gttagaacaa caaaaggcag ataaaaaagc tatttctgaa
    17281 aaacaagtag caaataatga tgctatcaat actgtaattg ctaatcaaca aaaattggct
    17341 gatgatgctc aagcattgac tacgaaacag gcagaactaa aagctgctga attaagtctt
    17401 gctgctgaga aagcgacagc tgaaggggaa aaagcaagtc tattagagca aaaagcagca
    17461 gctgaggcag aggctcgtgc agctgcggta gcagaagcag cttataaaga aaaacgagct
    17521 agccaacaac aatcagtact tgcttcagca aacactaact taacagctca agtgcaagca
    17581 gtatctgaat ctgcagcagc acctgtccgt gcaaaagttc gtccaacata tagtacaaac
    17641 gcttcaagtt atccaattgg agaatgtaca tggggagtaa aaacattggc accttgggct
    17701 ggagactact ggggtaatgg agcacagtgg gctacaagtg cagcagcagc aggtttccgt
    17761 acaggttcaa cacctcaagt tggagcaatt gcatgttgga atgatggtgg atatggtcac
    17821 gtagcggttg ttacagctgt tgaatcaaca acacgtatcc aagtatcaga atcaaattat
    17881 gcaggtaatc gtacaattgg aaatcaccgt ggatggttca atccaacaac aacttctgaa
    17941 ggttttgtta catatattta tgcagattaa tttacagagg gactcgaata gagccctctt
    18001 ttcaggtttt accgtgacaa tccctattaa aaattatatc aaaatagctt gaaaatattg
    18061 gaaaagtatg gtagaatgaa aattgtcgtg tgaacgataa tactcattct tgatgaattg
    18121 tgaagcagtt gcccttgggt cgttttgcga gttgaagtca agaagaggaa aaaaacaaaa
    18181 aggagaaata ctcatggcag taatttcaat gaaacaactt cttgaggctg gtgtacactt
    18241 tggtcaccaa actcgtcgct ggaatcctaa gatggctaag tacatcttta ctgaacgtaa
    18301 cggaatccac gttatcgact tgcaacaaac tgtaaaatac gctgaccaag catacgactt
    18361 catgcgtgat gcagcagcta acgatgcagt tgtattgttc gttggtacta agaaacaagc
    18421 agctgatgca gttgctgaag aagcagtacg ttcaggtcaa tacttcatca accaccgttg
    18481 gttgggtgga actcttacaa actggggaac aatccaaaaa cgtatcgctc gtttgaaaga
    18541 aattaaacgt atggaagaag atggaacttt cgaagttctt cctaagaaag aagttgcact
    18601 tcttaacaaa caacgtgcgc gtcttgaaaa attcttgggc ggtatcgaag atatgcctcg
    18661 tatcccagat gtgatgtacg tagttgaccc acataaagag caaatcgctg ttaaagaagc
    18721 taaaaaattg ggaatcccag ttgtagcgat ggttgacacc aatactgatc cagatgatat
    18781 cgatgtaatc atcccagcta acgatgacgc tatccgtgct gttaaattga tcacagctaa
    18841 attggctgac gctattatcg aaggacgtca aggtgaggat gcagtagcag ttgaagcaga
    18901 atttgcagct tcagaaactc aagcagattc aattgaagaa atcgttgaag ttgtagaagg
    18961 tgacaacgct taatttatac aaatagtaat tacctaggag ggcggggctt agcccggctc
    19021 tcctattttc aaaaatatag gagaattaaa atggcagaaa ttacagctaa acttgtaaaa
    19081 gagttgcgtg aaaaatctgg tgccggtgtt atggacgcta aaaaagcgct tgtagaaaca
    19141 gacggtgaca tcgaaaaagc gattgaattg cttcgtgaaa aaggtatggc taaggcagct
    19201 aagaaagctg accgtgttgc tgcagaaggt ttgactggtg tttatgttaa cggtaatgtt
    19261 gcagcagtta ttgaagtaaa tgctgaaact gacttcgttg caaaaaacgc tcaattcgtt
    19321 gaattggtaa atactacagc taaagtcatt gctgaaggaa aacctgctaa caatgaagaa
    19381 gctcttgctt tgataatgcc ttcaggtgaa actcttgaag ctgcatacgt atctgcaaca
    19441 gcaactatcg gagagaaaat ctcattccgt cgctttgcat tgattgaaaa aacagacgca
    19501 caacactttg gagcatacca acataacggt ggacgtatcg gtgttatttc agttgttgaa
    19561 ggtggagacg aagcacttgc taaacaattg tcaatgcaca tcgcagcgat gaaaccaaca
    19621 gttctttctt acaaagaatt ggatgagcaa ttcgttaaag atgagttggc acaattgaat
    19681 cacgttatcg accaagacaa cgaaagccgt gcaatggtta ataaaccagc tcttccacac
    19741 ttgaagtatg gatcaaaagc tcaattaact gatgatgtta ttgctcaagc tgaagctgac
    19801 atcaaagctg aattggctgc agaaggcaaa ccagaaaaaa tctgggacaa aattatccca
    19861 ggtaaaatgg atcgcttcat gcttgataat actaaagttg accaagctta cacacttctt
    19921 gcacaagttt acatcatgga tgacagcaag acagttgaag cataccttga atcagttaac
    19981 gcttcggtag ttgagtttgc tcgctttgaa gttggtgaag gtatcgagaa agctgcaaac
    20041 gacttcgaag ctgaagttgc agctacaatg gcagcagcct tgaataacta ataatagaaa
    20101 ggaagcaaat tgcttccttt tttgtatagg gcttagattt ttcctgaaat tgttttttcc
    20161 ccagtctcac tcgattagaa taattaaaac aaaaagccct ataataaggg ctaagtgcat
    20221 ttaggagact atacttcaaa ttcatagtaa gcgcgtcata acaaggtatc tatcctaatt
    20281 ccccaacttc cagtatacta gataaaaaat aattatcgga ggaaaggtat tgtactatga
    20341 taattcaact aagtgattta ggtcaagttc accttgtttg tggcaagaca gatatgaggt
    20401 agggaataga ctcattagcc tatgtactta aaacccactt tgaattggat tctttctccg
    20461 gtcaagtctt tctcttttgt ggtggacgta aagaccgctt taaagtcctt tactgggatg
    20521 gtcaaggatt ttggctacta tataaacgct ttgagaacgg aaaactgact tggct
//
LOCUS       DAVBCN010000033        20510 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVBCN010000033 DAVBCN010000000
VERSION     DAVBCN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20510
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.033"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            210..623
                     /gene="rpsL"
                     /locus_tag="VQ497_001115"
     CDS             210..623
                     /gene="rpsL"
                     /locus_tag="VQ497_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET2517429.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            643..1113
                     /gene="rpsG"
                     /locus_tag="VQ497_001116"
     CDS             643..1113
                     /gene="rpsG"
                     /locus_tag="VQ497_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET2517430.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            1538..3619
                     /gene="fusA"
                     /locus_tag="VQ497_001117"
     CDS             1538..3619
                     /gene="fusA"
                     /locus_tag="VQ497_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET2517431.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     SKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            3723..8114
                     /locus_tag="VQ497_001118"
     CDS             3723..8114
                     /locus_tag="VQ497_001118"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HET2517432.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFQTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPVEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNEIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNDYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            8216..8479
                     /locus_tag="VQ497_001119"
     CDS             8216..8479
                     /locus_tag="VQ497_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HET2517433.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQVLTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            8472..8750
                     /locus_tag="VQ497_001120"
     CDS             8472..8750
                     /locus_tag="VQ497_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HET2517434.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            8771..8923
                     /locus_tag="VQ497_001121"
     CDS             8771..8923
                     /locus_tag="VQ497_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517435.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            8945..10186
                     /locus_tag="VQ497_001122"
     CDS             8945..10186
                     /locus_tag="VQ497_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET2517436.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEGFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENVGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            10196..10426
                     /locus_tag="VQ497_001123"
     CDS             10196..10426
                     /locus_tag="VQ497_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET2517437.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            10637..11359
                     /locus_tag="VQ497_001124"
     CDS             10637..11359
                     /locus_tag="VQ497_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET2517438.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVMDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            11576..12910
                     /gene="pepC"
                     /locus_tag="VQ497_001125"
     CDS             11576..12910
                     /gene="pepC"
                     /locus_tag="VQ497_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HET2517439.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQVTVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAK"
     gene            complement(12966..13877)
                     /locus_tag="VQ497_001126"
     CDS             complement(12966..13877)
                     /locus_tag="VQ497_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2517440.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(13901..14704)
                     /locus_tag="VQ497_001127"
     CDS             complement(13901..14704)
                     /locus_tag="VQ497_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HET2517441.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(14732..15721)
                     /gene="manX"
                     /locus_tag="VQ497_001128"
     CDS             complement(14732..15721)
                     /gene="manX"
                     /locus_tag="VQ497_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HET2517442.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            complement(15954..16973)
                     /gene="adhP"
                     /locus_tag="VQ497_001129"
     CDS             complement(15954..16973)
                     /gene="adhP"
                     /locus_tag="VQ497_001129"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HET2517443.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKNLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            complement(17312..18124)
                     /locus_tag="VQ497_001130"
     CDS             complement(17312..18124)
                     /locus_tag="VQ497_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2517444.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            18314..19732
                     /locus_tag="VQ497_001131"
     CDS             18314..19732
                     /locus_tag="VQ497_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HET2517445.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLTMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNDENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            19785..20492
                     /locus_tag="VQ497_001132"
     CDS             19785..20492
                     /locus_tag="VQ497_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2517446.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLVLSYLVIICSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
BASE COUNT         5971 a         4024 c         4673 g         5842 t
ORIGIN      
        1 cagaaattga tctctaacat taccaataaa aatgaaaaat atcttgacaa gcaagggaaa
       61 attttgttac aataatagac ggtacttttt acttttggtc tctcaagagt gtacagggac
      121 gtgctgacaa atgttgcaaa agtacacaca gatgatatct gtcaccaagt gtatcatcac
      181 caaaaataaa aaaacacagg agaatgtaga tgcctacaat taaccaattg gttcgcaaac
      241 cgcgtaaatc aaaagtagaa aaatctaaat caccagcttt gaacgttggt tacaatagtc
      301 ataaaaaagt tcaaacaaac gtttcttcac cacaaaaacg tggtgttgca actcgtgttg
      361 gaacaatgac acctaaaaaa cctaactcag cccttcgtaa attcgctcgt gtacgtttga
      421 gcaaccttat cgaagttact gcctacatcc caggtatcgg acacaacttg caagagcaca
      481 gcgtggtgct tcttcgcggt ggacgtgtaa aagaccttcc aggggtacgt taccatatcg
      541 tccgtggtgc acttgatact gcaggtgtta acgatcgtaa acaaggccgt tctaaatacg
      601 gtactaaacg tccaaaagca taaggaaagg ggataaagag aaatgagtcg taaaaataga
      661 gctccaaaac gtgacgtatt gccagatccg ctatacaatt cacaactagt tactcgtctt
      721 atcaaccgcg ttatgcttga tggtaaacgt ggtactgctg cttcaatcgt ttacggtgct
      781 tttgagcaaa tcaaagaagc tactggcaac gatgcacttg aagtatttga aacagctatg
      841 gaaaacatca tgcctgtact tgaagtacgt gcacgtcgtg ttggtggttc taactaccaa
      901 gtcccagttg aagttcgtcc agaacgtcgt acaacacttg gacttcgttg gttggtaaca
      961 atcgctcgtc ttcgtggtga acacacaatg caagaccgtc ttgcaaaaga aatcttggat
     1021 gctgctaaca acactggtgc agctgttaag aaacgtgaag atactcaccg tatggctgaa
     1081 gctaaccgtg cattcgcaca cttccgttgg taagatagga tgcgaaagcg ttaagaaagt
     1141 cccagagaaa atagggaatc gaagcaggtt gcggttgcaa ccaatgagat tcatcttttt
     1201 ctccagactt ttagcttgag ctcaactaaa tcatgatgct aggcacggta aggatgcaag
     1261 gtgaaaatag gaaactgacg cagtattcaa cgaatacaag gagttttatc tttttcacgc
     1321 agcatcccgt tccagctcac atcggctaac taactttagc ccgggttcaa attagctaaa
     1381 tcgattagta ttagctataa ctcagcttac catctcgtaa gttgaaacca acaatagcat
     1441 gaaaacattg agaacgggta ggtcctgcct atccgttttt attaaaatcg tgttataata
     1501 gaatagaaat caaaaataaa taggagaaac aaacctcatg gcacgcgaat tttcacttga
     1561 aaaaactcgt aatatcggta tcatggctca cgtcgatgcc ggtaaaacaa caactactga
     1621 gcgtattctt tactacactg gtaaaatcca caaaatcggt gaaactcacg aaggtgcgtc
     1681 acaaatggac tggatggagc aagagcaaga acgtggtatc acgatcacat ctgctgcgac
     1741 aacagctcaa tggaacaacc accgcgtaaa catcatcgac acaccaggac acgtggactt
     1801 cacaatcgaa gtacaacgtt ctcttcgtgt attggatggt gcggttaccg ttcttgactc
     1861 acaatcaggt gttgaacctc aaactgaaac agtttggcgt caagcaactg agtacggagt
     1921 tccacgtatc gtatttgcca acaaaatgga caaaatcggt gctgacttcc tttactctgt
     1981 aagcacactt cacgatcgtc ttcaagcaaa tgcacaccca atccaattgc caatcggttc
     2041 tgaagatgac ttccgtggta tcattgactt gatcaagatg aaagctgaaa tctatactaa
     2101 cgaccttggt acggatatcc ttgaagaaga catcccagct gaataccttg accaagctca
     2161 agaataccgt gaaaaattga ttgaagcagt tgctgaaact gacgaagaat tgatgatgaa
     2221 atacctcgaa ggtgaagaaa tcactaacga agaattgaaa gctggtatcc gtaaagcgac
     2281 tatcaacgtt gaattcttcc cagtattatg tggttcagcc ttcaaaaaca aaggtgttca
     2341 attgatgctt gatgcggtta tcgactacct tccaagtcca cttgacatcc cagcaatcaa
     2401 aggtattaac ccagatacag acgctgaaga aactcgtcca gcatctgacg aagagccatt
     2461 tgcagctctt gccttcaaga tcatgactga cccattcgta ggtcgtttga cattcttccg
     2521 tgtttactca ggtgttcttc aatcaggttc atacgtattg aatacttcta aaggtaaacg
     2581 tgaacgtatc ggacgtatcc ttcaaatgca cgctaacagc cgtcaagaaa tcgacactgt
     2641 ttactcaggt gatatcgctg ctgccgttgg tttgaaagat actacaactg gtgactcatt
     2701 gacagatgaa aaatctaaaa tcatccttga gtcaatcaac gttccagaac cagttatcca
     2761 attgatggtt gagccaaaat ctaaagctga ccaagacaag atgggtatcg cccttcaaaa
     2821 attggctgaa gaagatccaa cattccgcgt tgaaacaaac gttgaaactg gtgaaacagt
     2881 tatctcaggt atgggtgaac ttcaccttga cgtccttgtt gatcgtatgc gtcgtgagtt
     2941 caaagttgaa gcgaacgtag gtgcgcctca agtatcttac cgtgaaacat tccgcgcttc
     3001 tactcaagca cgtggattct tcaaacgtca gtctggtggt aaaggtcaat tcggtgatgt
     3061 gtggattgaa tttactccaa acgaagaagg taaaggattc gaattcgaaa acgcaatcgt
     3121 cggtggtgtg gttcctcgtg aatttatccc agcggttgaa aaaggtttgg tagaatctat
     3181 ggctaacggt gttcttgcag gttacccaat ggttgacgtt aaagctaagc tttatgatgg
     3241 ttcatatcac gatgtcgact catctgaaac tgccttcaag attgcggctt cactttccct
     3301 taaagaagct gctaaatcag cacaaccagc tatccttgaa ccaatgatgc ttgtaacaat
     3361 cactgttcca gaagaaaacc ttggtgatgt tatgggtcac gtaactgctc gtcgtggacg
     3421 tgtagatggt atggaagcac acggtaacag ccaaatcgtt cgtgcttacg ttccacttgc
     3481 tgaaatgttc ggttacgcaa cagttcttcg ttctgcatct caaggacgtg gtacattcat
     3541 gatggtattt gaccactacg aagatgtacc taagtcagta caagaagaaa ttattaagaa
     3601 aaataaaggt gaagactaat ccgtcctcac tctagaagga agtcacttag tggcttcctt
     3661 ttgtctttag aaaatacctc taaatatggt aaaatagtag aagaataatg tgaggaaaat
     3721 gaatgtcaaa tagttttgaa attttgatga atcaattggg gatgcctgct gaaatgagac
     3781 aggctcctgc tttagcacag gccaatattg agcgagttgt ggttcataaa attagtaagg
     3841 tatgggagtt tcatttcgta ttttctaata ttttaccgat tgaaatcttt ttagaattaa
     3901 agaaaggttt gagcgaagaa ttttctaaga caggcaataa agctgttttt gaaattaagg
     3961 ctcggtctca agaattttca aatcagctct tgcagtccta ctatagggag gctttctctg
     4021 aaggtccatg tgctagtcaa ggttttaagt ccctttatca aaatttgcaa gttcgtgctg
     4081 agggtaatca gctatttatt gaaggatctg aagcgattga taaggaacat tttaagaaga
     4141 atcatcttcc taatttagcc aaacaacttg aaaagtttgg ttttcaaact tttaactgtc
     4201 aagtcgagaa gaatgatgtc ctgacccaag agcaggaaga ggcctttcat gctgaaaatg
     4261 agcagattgt tcaagctgcc aatgaggaag cgctccgtgc tatggaacaa ctggagcaga
     4321 tggcacctcc tccagtggaa gagaaaccag tctttgattt tcaagcgaaa aaagctgcag
     4381 ctaaacccaa actggataag gcggagatta ctcctatgat cgaagtgacg acagaggaaa
     4441 atcgtctggt atttgaaggg gttgtttttg atgtggagca aaaagtgact agaacaggtc
     4501 gtgttttaat caactttaaa atgacggact atacttcaag tttttctatg caaaagtggg
     4561 ttaaaaacga ggaagaggcc cagaagtttg acctcatcaa gaagaattct tggctccgag
     4621 ttcgagggaa tgtggagatg aataacttca cacgcgattt gactatgaac gtacaggatc
     4681 tgcaggaagt tgttcactat gagcggaagg atttgatgcc agaaggtgag cgtcgggttg
     4741 agtttcatgc tcatactaac atgtcgacta tggatgcttt gccagaggtc gaagagattg
     4801 ttgcaacagc tgctaagtgg ggacacaagg cggttgctat cacagaccat gggaatgtcc
     4861 agtcctttcc acatggctat aaggcggcta agaaagcggg aatccagctg atctatggga
     4921 tggaagccaa tatcgtggag gaccgtgtcc ctatcgtcta taacgaagtg gagatggact
     4981 tatcagaagc aacctacgtg gtctttgacg tggaaacgac gggactttca gctatctata
     5041 atgacttgat tcaggttgcg gcctctaaga tgtacaaggg gaatgttatt gctgaatttg
     5101 atgaatttat caatcctggg catcccttgt cagcttttac tacagagtta actggaatta
     5161 cagatgatca tgtcaaaaat gccaaaccac tagaacaagt tttgcaagaa ttccaagaat
     5221 tttgcaagga tacggtccta gttgcccaca atgctacctt tgacgttggc tttatgaatg
     5281 ctaattatga gcggcatgac cttccaaaga ttagtcagcc agttattgat acgctggagt
     5341 ttgctagaaa cctctatcct gagtataaac gccatggttt ggggcctttg accaagcgtt
     5401 ttggtgtggc cttggaacat caccacatgg ccaactacga tgcggaagcg actggtcgtc
     5461 tgcttttcat ctttatcaaa gaggtagcag aaaaacatgg tgtgaccgat ttagctagac
     5521 tcaacattga tctaatcagt ccagattctt acaaaaaagc tcggatcaag catgcgacca
     5581 tctatgtcaa gaatcaggta ggtctaaaaa atatctttaa gctggtttcc ttgtctaata
     5641 ccaagtattt tgaaggagtg ccacggattc cgagaacggt tctagatgcc catcgagagg
     5701 gcttgatttt aggttcagcc tgttcagagg gtgaagtttt tgacgtggtc gtttctcaag
     5761 gtgtggatgc ggcggttgag gtggccaagt attatgattt tatcgaggtc atgccaccgg
     5821 ctatctatgc acccttgatt gccaaagagc aggtcaagga tatggaggaa ctccagacca
     5881 ttatcaagag tttgatagag gttggagacc gccttggcaa gcctgttctg gctacgggaa
     5941 atgttcacta tatcgaaccg gaagaagaga tttatcgtga aattatcgtc cgtagtttgg
     6001 gacagggtgc gatgattaat cgaactatcg gtcatggtga acatgcccaa ccagcaccac
     6061 ttccaaaggc tcattttcga acgactaatg agatgttgga tgaatttgcc tttttgggag
     6121 aggaactggc tcgtaaactg gttattgaaa acaccaatgc cttggcagaa atatttgaac
     6181 ccgttgaagt cgttaagggt gacttgtata cgcctttcat cgacaaggct gaagaaacag
     6241 ttgctgagtt gacctataag aaagcttttg agatttatgg aaatccgctg ccagatattg
     6301 ttgatttgcg gattgaaaaa gaattaacat ccatactggg gaatggattt gctgtgattt
     6361 atctggcatc gcagatgctg gtgcaacgtt ctaatgaacg gggttatttg gttggttctc
     6421 gtgggtctgt cggatctagt ttcgttgcga ccatgattgg gattacggag gtcaatcctc
     6481 tctctcctca ctatgtctgt ggtcagtgtc agtacagtga gtttatcaca gatggttcgt
     6541 acggttcagg atttgatatg ccccataagg actgtccaaa ctgtggtcac aaactcagta
     6601 aaaacggaca ggatattccg tttgagacct tccttggttt tgatggggat aaggttcctg
     6661 atattgactt gaacttctcg ggagaagatc agcctagcgc ccacttggat gtgcgtgata
     6721 tctttggtga agaatatgcc ttccgtgcgg gaacggttgg tacggtagct gccaagactg
     6781 cctatggatt tgtcaagggt tacgagcgag attatggcaa gttttatcgt gatgcagagg
     6841 tggaacgcct cgctcaagga gcggcgggtg tcaagcggac aacaggccaa cacccggggg
     6901 gaatcgttgt tattccgaac tacatggatg tctacgattt tacgcctgtc cagtatccag
     6961 cagatgatgt cacggctgaa tggcagacca ctcactttaa cttccacgat atcgatgaga
     7021 acgtcctcaa actcgatgta ctgggacatg atgatccgac catgattcga aaacttcagg
     7081 atttgtctgg tattgaccct aatgaaattc ctatggatga cgaaggcgtg atggcactct
     7141 tttctgggac tgatgtgcta ggggtaacac ctgaacaaat tggaacgcct acgggtatgt
     7201 tggggattcc agagtttgga acaaatttcg tacgtggaat ggtagacgaa acccatccga
     7261 caacctttgc ggaattgctt cagctgtctg gtctgtcaca cggtactgac gtttggttgg
     7321 ggaatgctca ggatctgatt aagcaaggaa tagcggacct atcgactgtt atcggttgtc
     7381 gggacgacat catggtttac ctcatgcatg cgggtctgga acctaagatg gcctttacca
     7441 ttatggaacg ggtacgtaag ggtttgtggc taaagatttc agaagaggag agaaatgact
     7501 atatcgaagc catgaaggct aataaggtgc cagagtggta tatcgaatcc tgtgggaaaa
     7561 ttaagtacat gttccctaag gcccatgcgg cagcctacgt tatgatggcc ttgcgtgtag
     7621 cttacttcaa ggttcaccat cctatttatt actactgtgc ttacttctcc attcgtgcta
     7681 aggcttttga tatcaagacc atgggtgcgg gcttggaggt catcaagcgc agaatggaag
     7741 aaatctctga aaaacggaag aacaatgaag cctctaatgt ggaaatcgat ctctatacaa
     7801 ctcttgagat tgtcaatgag atgtgggaac gaggtttcaa gtttggtaaa ttagatctct
     7861 accgtagtca ggcgacagag ttcctcatcg acggggatac ccttatccca ccatttgtag
     7921 caatggatgg tctgggagag aacgttgcca agcaactggt gcgggcgcgt gaagagggag
     7981 aattcctctc taaaacagaa ctacgcaagc gtggtggact ctcatcaacc ttggttgaaa
     8041 agatggatga gatgggtatt cttggaaata tgccagagga taaccagttg agtttgtttg
     8101 atgagttgtt ttaaaaaatt gcttaataat ctattaaaag aggctaacgt atatccaata
     8161 gatttacatt agctttcttt tttgttaaaa tagtctatgg aaatagggtg agagtatgtc
     8221 aaagatgagt ataagcatcc gtctggatag tgaggttaag gagcaggccc aacaggtgtt
     8281 tagtaatctg ggaatggata tgacaacagc tattaatatt ttccttcgtc aggcaattca
     8341 atatcaggga ttaccttttg atgttagact agacgaaaat cggaagttgc tccaagtgtt
     8401 aacagattta gaccaaaatc gtaatatgag ccagtctttt gaatcagtct cagatttgat
     8461 ggaggactta cgtgcttaag attcgttatc ataaacagtt taaaaaagat tttaagttgg
     8521 ctatgaagcg tggtttgaag gccgaattat tagaagaagt tttgaatttt ctggttcaag
     8581 aaaaagaaca tcctgccaga tatcgtgatc attcattgac ggcatccaag cattttcaag
     8641 gagttcgtga atgccatacc cagccagatt ggcttttggt ttataaagta gacaagtcgg
     8701 aattgatttt aaatttgctg aggacaggca gtcacagtga tttattttaa tctattttaa
     8761 gggggttctc atgaaactaa gaatatttgc ggaagataag ccggctaaga aggtatttga
     8821 atatcaatta gaacttgctg atcgtacaat tcttctatcg acagcactct tgtcaggtgc
     8881 tattgcttta gcaggaatct tttctgcctt gaaagaaaaa taaaaataga aaagagaaaa
     8941 cagaatggtt ttaccaaatt ttaaagaaaa tctagaaaaa tatgcgaaat tgttggttgc
     9001 gaacggaatt aacgtgcaac ctggtcacac tttggctctc tctattgatg tggagcaacg
     9061 tgaattggca catctaatcg tgaaagaagc ttatgccttg ggtgcgcatg aggtcatcgt
     9121 tcagtggaca gatgatgtca ttaaccgtga gaaattcctc catgccccga tggagcgttt
     9181 ggacaatgtg ccagaataca agattgctga gatgaactat cttttggaga acaaggctag
     9241 ccgtcttggg gttcgttcat ctgatccagg tgccttgaac ggagtggacg ctgacaagct
     9301 ttcagcttct gctaaagcta tgggacttgc catgaagcct atgcgtatcg caactcaatc
     9361 taacaaggtt agctggactg tagcagctgc agcaggactt gagtgggcta agaaagtctt
     9421 cccaaatgct gcgagcgacg aagaagcagt tgatttcctt tgggaccaaa ttttcaaaac
     9481 ttgccgtgtc tatgaagcag atcctgttaa ggcttgggag gaacatgcag ccattctcaa
     9541 gagcaaggca gatatgctta ataaggagca attttcagcc cttcactaca cagcgccagg
     9601 aacagattta acacttggtt tgccaaagaa ccacgtttgg gaatcagctg gtgctgtcaa
     9661 tgcacagggc gaaggattct tgccaaatat gccgacagaa gaggtcttca cagcgcctga
     9721 cttccgtcgt gcagatggtt atgtcacttc tacaaaaccg cttagctaca acggaaatat
     9781 cattgaaggc attaaggtga cctttaagga tggacaaatc gtagatatca ctgctgagaa
     9841 gggtgatcag gttatgaaag accttgtctt tgaaaatgtg ggtgcgcgtg ccttgggtga
     9901 atgtgccttg gtaccagatc caagtccaat ttctcagtca ggcattacct tctttaacac
     9961 ccttttcgat gaaaatgcgt caaaccactt ggctatcggt gcagcctatg cgactagcgt
    10021 tgttgatgga gcggagatga gcgaagagga gcttgaagct gcagggctta accgttcaga
    10081 tgttcacgta gactttatga ttggttctaa ccaaatggat atcgatggta ttcgtgagga
    10141 tggaacgcga gtacctcttt tccgtaatgg gaattgggca aattaaggag ataatatgtt
    10201 aggaagtatg ttcgttggtc tcctagtggg atttttagca ggtgctatga ccaatcgtgg
    10261 agagcgaatg ggatgttttg gaaaaatgtt tctcggttgg atcggagcct ttctaggtca
    10321 cttgctcttt ggaacttggg ggccagtttt atcaggaaca gctattatcc cagcgatttt
    10381 aggagccatg attgttttag ctattttttg gagacgagga agttaatttc ctaaatctga
    10441 tactcaatga aaatcaaaga gcaaactagg aaactagccg caggttgctc aaagcactgc
    10501 tttgaggttg cagatagaac tgacgaagtc agtaacatat atacggcaag gcgacgttga
    10561 cgcggtttga agagtatgaa acgaaaagga ggttggtcat tgtaccagcc tccttttgat
    10621 atgatataat agttctatga gattagataa atttttagtt gcctgtgcgg tgggaagtcg
    10681 gactgaggtc aaaaacttgc tcaaggctgg gcgcgtgact gtaaatggta aaaaagaaaa
    10741 atcagctaaa ttgcagattg atgaaaaaat agatgagatt cgctttgatg ggcaagtgtt
    10801 ggagtatgaa gagtttgtct actacatgat gaacaagccc aaaggagtta tctcagcgac
    10861 tgaggatccc aagcacagaa ccgttatgga cttgttggat gacttggcgc ggagtaagga
    10921 agttttccca gtaggacgct tggatattga cacgcatggt cttttgctct tgaccaatga
    10981 tggtcagctg gctcatgttc ttctttcgcc caagcgtcat gtggacaaga cttatctggc
    11041 acaagtcaag ggaatcatga cccaagaaga tgtggagaca tttgctgagg gtattcctct
    11101 caaagacttt acctgtcaac ccgctatact ggagcttgta tccatagata cagaaaagaa
    11161 tcaaagccaa atccgtgtga ccattgcaga agggaagttt catcagatca agcgtatggt
    11221 gggctactgt ggcaaggaag tggtggactt gcaacgattg actatgggaa ctttagtatt
    11281 agatgagaac ctggaacgag gggaatggcg tcgcttgacc aaggaagaat tagaaattct
    11341 ccgcgctaat attatttaat ttagtttaat actcaatgaa aatcaaagag caaactagga
    11401 aactagccgc aggctgtact tgagtacggc aaggcgacgt tgacgtggtt tgaagagatt
    11461 ttcgaagagt ataaattcga aaaaggtgag ggacaatgtc ctcgcctttt atgtttttta
    11521 gttgcttcct ttgtgaaaag agttataata gactgtagaa taaaaggagg aatctatgaa
    11581 cgcgattcaa gaatcattta ctgataaact atttgccaac tatgaagcaa atgtaaaata
    11641 ccaagcgatt gaaaatgctg ccagccacaa cggaattttt gcagctctgg aacgtcgcca
    11701 aagccatgta gacaacacac ctgttttctc attggattta accaaggaca aggtcactaa
    11761 ccagaaagcg tctggtcgtt gctggatgtt tgcggctctc aacaccttcc gccacaaact
    11821 catctcgcaa tacaaattgg agaactttga gttgtcacaa gcccacactt tcttctggga
    11881 taagtatgag aaatcaaact ggttcttgga gcaagtcatt gcgacttcag accaagaatt
    11941 gactagccgc aaggttagct tcttactcca aacacctcaa caagatggtg gtcaatggga
    12001 tatggtcgtt tccctctttg aaaaatacgg tgtcgtgcct aagtcagttt atcctgagtc
    12061 tgtttcatct agcagcagtc gtgagctaaa tgcgatcctt aataaattgc ttcgtcaaga
    12121 tgctcaaatc ttgcgtgatt tgcttgtttc tggtgcagat caagtgactg ttcaagctaa
    12181 gaaagaagac ctcttgcaag aaatctttaa ctttcttgct atgtcattag gacttccacc
    12241 acgcaagttt gactttgctt atcgcgataa agataacaac tacaaaagtg aaaaaggaat
    12301 cacaccacaa gagttctaca agaaatatgt caatcttcct ttagaagact acgtttctgt
    12361 tatcaatgct ccaactgctg ataaacctta cggaaaatct tacacagttg agatgttggg
    12421 gaatgtggtt ggtagccgtg cagttcgtta catcaacgtt ccaatggagc gcttgaaaga
    12481 attggcgatt gcccaaatgc aagcaggtga gactgtttgg tttggttctg atgtcggcca
    12541 gctcagtaac cgtaaggctg gaatccttgc gacagatgtt tatgactttg aatcaagcat
    12601 ggacattaaa cttactcaag acaaggctgg acgtttggac tatagcgaaa gcttgatgac
    12661 ccacgccatg gtcttgacag gtgttgactt ggacgaaaat ggtaaatcaa ccaagtggaa
    12721 ggttgaaaac tcatggggag acaaggtcgg tacagatggt tactttgttg cctcagacgc
    12781 ttggatggac gaatacacat accaaatcgt tgttcgtaag gaattgctga cagcagaaga
    12841 acaagctgcc tatggagcag aaccaattgt acttgcacca tgggatccaa tgggagcctt
    12901 ggctaaataa aagcatagaa aaaaggaatc agattttaga acctggttcc tttttagttg
    12961 cttgattaca tgatgtgaag aacatgtgcc acaataccca ctgcgaagag ggcaaggata
    13021 atagtgattg gagatacttt tttcttaagt aagtacatgc aaagtaaagt aaggagtagt
    13081 cctgataatc caggaatcaa catatccaag ttttgttgga aagtagtaac tttttcagga
    13141 gtttgagaca atccttgtcc tacttgtgcg aatgcttctt ggataccttt agacccttct
    13201 ggcaatttat cccaatggat ataagccttt tcatctagtt gaactttaga aacatcgaaa
    13261 gcaaatttaa tatttaccca gcgttgaaca aggacagcaa gaatgaacat cccaaggata
    13321 gaagctcctt tagtgatatc ttgaaggata ccaccagaca tatctttagt gatttctgat
    13381 ccagccttgt atccaatctc ttgaacatac cacaagaatg acatacgaat caagttccat
    13441 gcaacaaaga agaggagtgg ccccaagata ttgccagtaa gggcaagtga agcaccgaga
    13501 gatccaagga ttgggcgtac tgtaaaccag aatactgggt caccgatacc agcaagaggt
    13561 cccatcatac cgattttaac cccttggata gcagcgtcat cgatttccac accgttagca
    13621 cgttcttctt caagcgcaag agtaaccccc atgactggag cagctacgta tggatgagtg
    13681 ttgaagaact caaggtgacg ctcaagagca gcgatttgat cttctttttt agtatagagt
    13741 tttttgatag ctggaatgag tgtataagcc cagcccaagt tttgcatccg ttcaaagttc
    13801 caagaccctt gtaagaaggt tgaacgccac caaacttttt tacgatctga tttagttaat
    13861 tgaagttttt cagtcatgat gttttcagtc ctttcttatc ttagtagtct tctaggatat
    13921 cgccgattgg gtcgttagaa gttgcggctc ctccgccacc atttccacca gttttagaaa
    13981 ggtgaaggta gataagagcg atagcaacac cgatagcacc gaatccgatt agagtaatat
    14041 ctgacacagc agcgagaacg aaaccaagag cgaagaatgg ccatacttca cgagttgcca
    14101 tcatgttgat aaccatggcg taaccaacgg caacgaccat accaccaccg atagccatac
    14161 catctttgag ccagtctggc atggcactaa ggatactttg tacagtttca gttggtacca
    14221 taaggagaag agctgcagga agcgcgatac gaagtccttg gaagagtagc gcaatgaaat
    14281 gcgcacgctc cacagcgccg aagtcacctt ttttagcggc agcatctgca gtatgaacca
    14341 aaccaactga aattgtacga acaatcattg tcaagaaaag tccagctacg gcaagaggga
    14401 tagcaaccgc ttgggcaaca ccgataccag tcttggtaaa gtcaccacca agaaccatga
    14461 taatggcagc agcgacagaa gcaagtgcag catcaggagc gatagcagca ccgatatttg
    14521 accaaccaag ggcaatcatt tgaagcgatc caccgaggat aatccctgct tccaagtgac
    14581 ctgttacaag cccaataagg gtacaggcta caagtggttg gtgaaattgg aactggtcga
    14641 ggatgccttc aagacctgca aagaaggcta caacgactac taaaaccata gaaataatag
    14701 acatgtttaa aatcctttca taaataatgg cttatttgac attggctttg ttaatcaagt
    14761 caaacaaatc ttttttagaa tcatttggta ctttacggac atcaaattca acacccaagt
    14821 cacgcatttt ttcaaatgta gcaacatctt ctttgtccat agacaaaacg gtattgacca
    14881 atgttttacc tgttgagtga gccatagaac caacgttaag agtcttgatt ggcacgccgc
    14941 cttcgatggc acgaagggca tcttgaggtg tttcaaacaa gataagggca tgtgtttctc
    15001 caaaacgtgg gtcttttgaa atctcaatca gtttttgaat tggaaccacg ttagccttga
    15061 cattacctgg agctgcttgt ttaatcaatt ctttacgaag gtcgtcttta gccacgttat
    15121 ctgaagcaac gatgatacga tttgcttttg aatctggagt ccaagcagtt gcaacctgac
    15181 cgtgaagtag acgtgtgtca agacgggcaa gattgatttt caatttaccg tctccgataa
    15241 cagttccttc tgggatagca gtttgggcaa ctggagcagc tgcagcgctt gcaacttctt
    15301 caactggatt tagctcttct ggaagagctt tgatgccatc tttggcttct ttaatgatat
    15361 tagcagcgac tttttctaca cctgcagcag cgtccatgag gcgctctgtg taggcttgaa
    15421 tcaacatcgg taagttaagt cctgtgatga tggcaaactt acgctcagga ttttctccca
    15481 tcacgcgact agcttggtta aatggagaac cactccaaag gtcagccaaa actagaacct
    15541 catcttctgc gtcaaatgca gcaacagcgt tattaaactt agcgtataga tcatcaggac
    15601 cttcatttgg cataaaggtc acaacttgaa ccttttcttg ttcaccaaag atcatagatc
    15661 ctgactgatg aatacccgca gcaaattcgc cgtggctcgc aataatgatt ccgatactca
    15721 ttattgtcat tcctcctttt ttgttttagt tttcatttaa gaaaacttta agactgttta
    15781 agtataaacc gttttcataa aaaaagcaac tatttatcat aaaaaattaa aaaattttta
    15841 tttttgtatg ttgatatgtt gagtttttga ggagtaatct tgaattctta tttaaaattt
    15901 aatataaaaa agttggaaga agcttccaac ttttaaaata ggtttctatt agattagtgg
    15961 gtgaagtcga gtaccatacg tccttggatt tggccttttt ccatttcgtc gaaaatggca
    16021 acagcatctt ctactggacg tttttgaaca actgggacta ccagaccttc tgcaccaaat
    16081 tggaaggctt cttctaagtc tttacgagtt ccgacaagag aaccgatgac ttggattcca
    16141 tcgaggactg ttttaacgat gctgagttcc atcatttcag aaggaagacc aacagcgacg
    16201 acgcgaccac cagcacgaat ggagtcaaca gcctggttga aggcaacttt agacacagca
    16261 gttacgacag ctgaatgagc tcctccatca gttttttctt taatgagtcc agctacatct
    16321 tcaacttcga ggccgttaat cacaatgtca gcgcctactt cttttgcaag ggcaagtttg
    16381 tcattgttga tatcgactgc gataacatga gcattgaata cttttttagc gtattgaaca
    16441 gcgaggttac caagtccacc agcaccgtaa agaacaaccc attggcctgg ttcaactttt
    16501 gcttctttga tagctttata ggttgttact ccagcacatg tgatagaaga agcttgggct
    16561 ggatcaagtc cgtcaggaac tttgacagca tagtcagcag ttacgataca ttgttcagcc
    16621 ataccaccgt ctactgagta gccagcattt ttcactgtac ggcaaagggt ttcgcgacca
    16681 gttgtacagt attcgcaagt gccacatcct tcaaagaacc aagcaacgct gacgcggtca
    16741 ccgactttaa ggtttttcac atctggagca atctctttaa cgataccgat accttcgtgc
    16801 ccaagaacac gtcctgggac ttgaccaaag tcaccatgag caacgtggag gtcggtgtgg
    16861 caaacgccac agtattcaac ttctacaagt gcttccccag tttcaagtgg acggagtact
    16921 ttttcttcaa tagcaacacc agtgctttct ggatttacaa caacagcttt catagctatc
    16981 ctccttgata ttgaccgaga acagggacaa cgggactgga ttgatttttt gtcaacagag
    17041 tcgagtgtga cgagctctct tgtatttata tgtgaagtat atcacaaaag aaagcctttt
    17101 ccaaggtttt ggagacaaaa agtagaacaa tcgattaact ggttgatgag actgtgaaaa
    17161 gagcacaaag accagcttgc aagctggtca tactcttcga aaatctcttc aaaccacgtc
    17221 agcttcatct acaacctcaa agcagtgctt tgagcaacct acggctagct tcctagtttg
    17281 ctctttgatt ctcattgagt atcagtatag gttataagaa taagtcttgt agggttttgg
    17341 caaccccatc ttggtcgttg gtaagggaaa tttgctcatc tgcataaggg agtagctctg
    17401 gattggcatt tttcatggca taacccttcc cagcaaaagc gagcatttcg gtatcattgt
    17461 gttcatctcc aaaggcaatc aaatcttttt tgtcacgatt cattatcttg agcaagtagt
    17521 ccaaagcaaa ggccttgttg acaccttttg gggtacattc aaggatattg agcggacctc
    17581 cccaggtatt gatagaaagt tgatgctggt agaaggcgtt catttctttt gccaaggcat
    17641 atttgtcact ggctctggtc tgcaagagga tacagttagg gtccttggtc accaattcag
    17701 gctggaattg atcttcaggc tggaaagctt ctacaccaaa tagtttggga ttggcaattt
    17761 cttcattggg atttgtaatg tagaattttt tacgatattc tccagcgata aaatcggctt
    17821 gaatgtcctc tgaacgttga accatatcta gcagatattt tttgtctaca gtcaaacact
    17881 tttcaaaatc ccaaacttgg tctggtaaat gagtaaggga tccgttgaag ttaatcatag
    17941 gagtgtctaa gcccagttca cggtaaaaat cttttgacat acggtaaggg cgacctgtcg
    18001 taataataac ctgatggcct ttttcagcaa cttttttgat ggtttttttg gtaaagtcag
    18061 aaatttgact gtctgagttg agcagggttc catctaggtc aactgcaata atttttttcg
    18121 tcatatggac ctttctagtg tgttttcaga taagatgtct tctattataa cagaaagcag
    18181 gcagaaaagc agattccaaa caaaaaatga aattggatta atttcttaaa caaatcaaac
    18241 aaagacattg aaaaagtgaa tgataaatga tataattcta ttattgttcg taaaaattaa
    18301 aaggagattg atgatggaca aattatttaa actaaaagag aacggtacag acgttcgtac
    18361 agaggttctc gctggtttaa caactttctt tgcaatgagc tatattctct ttgtaaaccc
    18421 acaaatactt tcacaaacag gaatgcctgc tcagggtgtg tttcttgcaa cgattatcgg
    18481 tgcagttgct ggtaccttga cgatggcctt ctatgctaac ttgccatacg ctcaagcacc
    18541 aggtatggga ctcaatgcct tctttacctt tacagtcgta tttggacttg gttattcttg
    18601 gcaagaagcc ctagctatgg tcttcatctg tgggattatt tcattgatta ttaccttgac
    18661 aaatgttcgt aaaatgatca ttgaatcgat tcccaatgct cttcgctcag ctatttcagc
    18721 tggtatcggt gtcttccttg cctatgtagg gattaagaat gctggacttt tgaaattcac
    18781 gattgatcca ggcaactata ctgttgtagg agaaggggct gacaaagctc aagcaacgat
    18841 tgcagcaaac tcttcagcag ttccaggatt ggtcagcttt aataatccag ctgttttagt
    18901 ggctcttgca ggacttgcca ttactatctt ctttgtcatc aaagggatta aagggggaat
    18961 tattctctct atcttgacaa caactgttct tgctattgca gttggtttgg ttgatttgtc
    19021 tagtatcgat tttgctaata accatgttgg tgcagctttt gaagatttga agacaatctt
    19081 tggtgcagct cttggttcag aaggtttggg agctttggtt tcagatacag ctcgcttgcc
    19141 tgaaactctg atggccattc ttgccttctc attgacagat atttttgaca caattggtac
    19201 cttgatcggt acaggtgaaa aagttggtat tgtagcgaca aatgatgaaa atcaccaatc
    19261 agccaaattg gataaggctc tttactctga tttgattgga acgacagtcg gtgccattgc
    19321 aggtacttca aacgtaacga cttatgttga gtctgctgct ggtatcggtg caggtggacg
    19381 tactggtttg acagccttgg ttgtagctat ctgttttgcg atttcaagct tctttagccc
    19441 acttctagcg atcgtaccaa cagcggctac agctccaatc ttgattatcg ttgggattat
    19501 gatgcttggt agcttgaaaa atatccattg ggatgatatg tctgaagcag ttcctgcctt
    19561 cttcacatct atctttatgg gattcagcta ctctatcact caagggattg cagttggttt
    19621 cttgacttac actttgacta agcttgtcaa aggtcaagtt aaagatgttc atgccatgat
    19681 ttggattttg gatgccttgt ttatccttaa ctacatcagc atggccttat aatagaatga
    19741 cccaggggga tttccccctt tttttaatac aaaggagata ggtgatgaaa gagaaaaata
    19801 tgtggaaaga attgttgaat cgtgcaggct ggattttggt ctttttactt gccgtccttt
    19861 tatatcaggt tcccctagtg gttacctcta ttttgacttt aaaagaagta gccctgctac
    19921 agtcagggct gatagttgct ggcctttcaa ttgtggttct ggctctattt attatgggag
    19981 ctcgtaaaac caagttagct agttttaatt tttctttttt tagagctaaa gatttggcac
    20041 gtttggtctt gagttattta gtcattatct gttcaaatat acttggttct atcttgttgc
    20101 aactgtcaaa tgagacgaca acagctaacc agtctcagat taacgatatg gttcagaata
    20161 gttcgttgat ttctagcttc ttcttgctag ccttgcttgc tccaatttgt gaggaaatct
    20221 tgtgccgtgg gattgttcct aaaaagattt tccgaggcaa ggagaacttg ggatttgtag
    20281 tcggtacgat tgtgtttgct ttattgcatc aaccaagtaa tttaccttct ttattgattt
    20341 atggaggtat gtcgacagtt ctatcttgga cggcctacaa gacccaacgt ttggaaatgt
    20401 cgatcttgct tcacatgatt gttaatggga ttgctttctg tttgttggct cttgtggtga
    20461 ttatgagtcg gacattagga atttctgtgt aaaagttttt atgtaggaac
//
LOCUS       DAVBCN010000034        19776 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVBCN010000034 DAVBCN010000000
VERSION     DAVBCN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19776
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.034"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(52..1464)
                     /gene="sufB"
                     /locus_tag="VQ497_001133"
     CDS             complement(52..1464)
                     /gene="sufB"
                     /locus_tag="VQ497_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET2517447.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(1514..1954)
                     /locus_tag="VQ497_001134"
     CDS             complement(1514..1954)
                     /locus_tag="VQ497_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET2517448.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            complement(1941..3167)
                     /locus_tag="VQ497_001135"
     CDS             complement(1941..3167)
                     /locus_tag="VQ497_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2517449.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPASARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            complement(3178..4440)
                     /gene="sufD"
                     /locus_tag="VQ497_001136"
     CDS             complement(3178..4440)
                     /gene="sufD"
                     /locus_tag="VQ497_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET2517450.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKINYLERLESRGEGSAKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSYADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            complement(4468..5238)
                     /gene="sufC"
                     /locus_tag="VQ497_001137"
     CDS             complement(4468..5238)
                     /gene="sufC"
                     /locus_tag="VQ497_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET2517451.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITRLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            complement(5388..5582)
                     /locus_tag="VQ497_001138"
     CDS             complement(5388..5582)
                     /locus_tag="VQ497_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HET2517452.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            complement(5610..7265)
                     /gene="dnaX"
                     /locus_tag="VQ497_001139"
     CDS             complement(5610..7265)
                     /gene="dnaX"
                     /locus_tag="VQ497_001139"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET2517453.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            complement(7265..7762)
                     /locus_tag="VQ497_001140"
     CDS             complement(7265..7762)
                     /locus_tag="VQ497_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET2517454.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            complement(7848..9050)
                     /gene="rpsA"
                     /locus_tag="VQ497_001141"
     CDS             complement(7848..9050)
                     /gene="rpsA"
                     /locus_tag="VQ497_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET2517455.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(9212..9283)
                     /locus_tag="VQ497_001142"
     tRNA            complement(9212..9283)
                     /locus_tag="VQ497_001142"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9249..9251),aa:Gln,seq:ttg)
     gene            complement(9293..9373)
                     /locus_tag="VQ497_001143"
     tRNA            complement(9293..9373)
                     /locus_tag="VQ497_001143"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9337..9339),aa:Tyr,seq:gta)
     gene            complement(9423..9653)
                     /locus_tag="VQ497_001144"
     CDS             complement(9423..9653)
                     /locus_tag="VQ497_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HET2517456.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(9821..10465)
                     /gene="pcp"
                     /locus_tag="VQ497_001145"
     CDS             complement(9821..10465)
                     /gene="pcp"
                     /locus_tag="VQ497_001145"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET2517457.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(10480..11403)
                     /locus_tag="VQ497_001146"
     CDS             complement(10480..11403)
                     /locus_tag="VQ497_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HET2517458.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(11400..12089)
                     /locus_tag="VQ497_001147"
     CDS             complement(11400..12089)
                     /locus_tag="VQ497_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HET2517459.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(12154..12858)
                     /locus_tag="VQ497_001148"
     CDS             complement(12154..12858)
                     /locus_tag="VQ497_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2517460.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSDM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGFPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            complement(12914..13936)
                     /locus_tag="VQ497_001149"
     CDS             complement(12914..13936)
                     /locus_tag="VQ497_001149"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HET2517461.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(14068..16539)
                     /gene="parC"
                     /locus_tag="VQ497_001150"
     CDS             complement(14068..16539)
                     /gene="parC"
                     /locus_tag="VQ497_001150"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HET2517462.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(16959..18902)
                     /gene="parE"
                     /locus_tag="VQ497_001151"
     CDS             complement(16959..18902)
                     /gene="parE"
                     /locus_tag="VQ497_001151"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HET2517463.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            19038..19679
                     /gene="plsY"
                     /locus_tag="VQ497_001152"
     CDS             19038..19679
                     /gene="plsY"
                     /locus_tag="VQ497_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET2517464.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
BASE COUNT         5681 a         4496 c         3569 g         6030 t
ORIGIN      
        1 ggcgatgctg acgtggtttg aagagatttt cgaagagtat aaaatcaaat tttatccaac
       61 tgatccctcc atttcatagc taatcaagcg gttcagctca actgcgtatt ccattggaag
      121 ttcttttgta aagggttcta caaatcccat gacaatcatt tcagttgcct cagattctga
      181 caatccacgg ctcatgagat aatacaattg ctcttctgaa atcttagata ctttggcttc
      241 gtgttccaaa gccacttgcg agttgtgaat ttcattaaat ggaatagtat ctgatgctga
      301 caagtcatcc atgataatgg tatcacattc aatgtgggaa acagatttct tagagttctt
      361 gttaaaggtg acttgtccac ggtagtcaac ctttcctcca cctttagcga tggatttaga
      421 cacaatagac gagctggtat gtggagcatt gtgaatcatc ttagcacccg tgtcttggtg
      481 ttgccctgca ttagcaaagg cgatagagag catggtacca cgcgctcctt ctccatcaag
      541 ataaacagat ggatatttca tagtcgtttt ggcacccaag tttccatcaa tccactcaac
      601 agtggcatcc ttttgagcct tagcacgctt tgttaccaag ttatagacgt tatcagacca
      661 gttttggata gttgtataac gcatataagc tccgtccaaa gcaaaaattt ctacaatggc
      721 agcgtgcaag ctattgcttg aatatgttgg tgctgtacat ccttctacgt agtggacgct
      781 tgctccctca tcaacgataa tcaaggtacg ttcgaactga cctatatttt cgttattgat
      841 acggaaataa gtttgaagtg gaatatctac cttgacacct tttggcacgt agataaaagt
      901 tccacccgac catactgctg agttgagggc tgccaacttg ttatctgtcg gcggtaccaa
      961 cttcgcaaag tattgtttaa ataagtctgg gtattccttg agtgcggaat ctgtatctgt
     1021 aaagataata cctaattttt ggaactcttc cttcatgttg tggtaaacca cttctgactc
     1081 gtactgggca gaagcccctg ctaaataagc acgttcagct tctggaatcc cgatacgttc
     1141 aaaggtttct ttaatctttt caggtacatc atcccaagaa cgggctggtt tgtcagatgg
     1201 tttttggtag tagattaagt catcaaagtc aatctctgac aagtctgctc cccaagtttg
     1261 catgggcatt tttttgaagg tttcataaga cttcaaacgg aactccaaca tccactcagg
     1321 ctcaccctta gcagcagata attcacgaat aacaccttcg ttgagtcctt ttcctgtcga
     1381 taagacaggc tctacatcgt catggaaacc aaatttatat tcaccaaggt caattggttt
     1441 tggttctact ctttcttcag ccataatatc ctttcttcat ttctaataat tcataagaca
     1501 aaagaaactt gtcttactgt ttttcttgat tttcaattgt tttcttaagg gcattccaag
     1561 ctagggttgc acacttgatt ctttgaggga atttggcaac acctgacaag aatgccgcgt
     1621 ctccaagttg gtcttgacgc tcatcttttt gcccttgaac catttcagaa aaaatagtcg
     1681 ccagttctaa aatttcttgt ttggtttttc ctaaaacggc atctgtcatc atactagcag
     1741 aagcagttga aatcgtgcat cctgaattta gaaaagcaat atcttccaaa cggtcctctg
     1801 catcaaactt gacagagagg ttgatgacat ccccacaagt cggattgttg agactgattt
     1861 gctcagcgtc ttctaacttc ccttggtgat gtggattttt cgaatggtct gctaccactg
     1921 ccatataaag gctatctagt ttagaaagtg ccattgaaaa actcctttgt cttttgtagg
     1981 gcatcgacta gtttgtcgca atctgccttg gtattataga tataaaaact tgcacgagct
     2041 gatgctggga cttccaaata ctgaagcaag ggttgcgcac aatggtgacc agcacgaaca
     2101 gccactcctt cataatccag agccgtcgca agatcgtgag gatggagatc acctaggtta
     2161 aaggcaataa cacccgaacg ttgagccaaa tcctgagaac cgtaaatggt caatccctca
     2221 attgcctgca gttttggata gacgtacgca atcaattcct gttcatgagc ttcaacggca
     2281 tccataccaa tcttttccag ataatcaact gcagtcgcaa gtccaatagc tcctgccata
     2341 tttggcgttc cagcctcaaa tttccaaggc aattccttcc aactagcgga ttgctcgtag
     2401 acaaaatcaa tcatctcgcc gccaaattct actggagaca tttgctcaag atacttttct
     2461 ttgccgtaaa ggacaccgat accagtcgga ccagccatct tgtgacccga aaaggcgaaa
     2521 aagtccagat ccaagtcctg gacatcaatc ttcatatgag gtgtagattg agcaccatcc
     2581 actaccatga ttgccccaac ttggtgggct aattgagtga tttccttgat cggattgacc
     2641 acaccaagaa cattggaggc atgagctagg gaaacaaatt taaccttatc agtcaattta
     2701 gctcgcaaat cctccatatc caaggcaccg tctttaagat agacatagac aagctctgct
     2761 ccagtctttc gacaagcttc ctgccatgga atgatattag aatggtgttc cattactgaa
     2821 atcaagacct ggtctccctc agtgagaatt tcctcagcaa agcgtgccac ccagttaagg
     2881 ctggttgtcg ttcctctggt aaagagaact tcctttgtag agcctgcatt aataaactta
     2941 cgaatggttt cacgagcagc ttcataagaa gctgtcgctc gttccgctaa ggtatggaca
     3001 ccacggtgaa cattggcatt gtcctgctca tagtaactgt taatagcttt cagaactact
     3061 agtggttttt gtgtcgtcgc agcattgtcc agatagacca gaggttcatc attgacaatc
     3121 tgatctaaaa ttggaaaatc cttgcgaatc gcttctacat ctaacatagg cttcccctta
     3181 gcgttttgac aatttctctt cgatagttgc aatcatttca tcacgaactt ccttgactgg
     3241 aatctccacg ataacagatc caaggaaacc acgaacaacc aaacgctctg cagttgcctt
     3301 atccaatcca cgactcatga ggtaatacat gtcttctgga tcaacttgtc cgatagacgc
     3361 tgcgtgtcct gcagtgacat cattttcatc aatcaaaaga attgggttag catctgaacg
     3421 cgcttggtct gaaagcatga gaacacggct ctcttgttgg gcatctgctc ccttagcacc
     3481 cttgatgatg tggccgatac cattgaaagt caaagttgct ttttcaagga taaccccatg
     3541 ttgtaggata tttccgatag agttgcagcc atagttagtt acacgagtat caatcccttg
     3601 tacctgacga ccacttgaaa gagctacaac cttgaggtca gcatagctac cattaccaat
     3661 caagtcacta tcaaaatcag caacgacatt tccttcgttc atgacaccga tagcccagtc
     3721 aatacttgca tcgttgccta atttaccacg acggctaatg taggcagtga cgttttcacc
     3781 tagacggtcg atagcagcaa acttgacttg cgcacctgaa cgtgcaatca cttctactgt
     3841 gatattagca gttgctttgg cacttccttc accgcgtgac tctaaacgct ccagataatt
     3901 aatcttagaa tttttaccag cgataatcat aatatgcttg ttaaacggca cattgctatc
     3961 gctatcttgg tagaaaattc cttcaattgg ctctgtgatt tctacgttat ctggaatata
     4021 gagtacagca ccactgttaa agtaagctgt gtggtaagcc gccaacttgt catcatcata
     4081 cttaacagat gacatgaaga attcttcgat cagctctgga atttcttcta aagctgagtg
     4141 aaagtctgtg aagacaacac cctgttcagc taactcaact ggagtttgtt cgaaaacagt
     4201 ttgagttcct acttgcacca acttcaagtg atgatctaaa gctgtgaaat ctggaacatt
     4261 tgctgatggc tcattttctg taatcgttcc atcacccaga ttccaacggt ggaatttgac
     4321 acgctcaata actggtaatt ccaaagtctc aatcttgtca aaagcttttt gacggagatc
     4381 agccaaccag cttggttcag cgtgcatttc tgaaaaaagt ttaatatttt ctctagtcat
     4441 ttacttctcc taaaagatac gaggaaatta caattcttcc ttgtagtcgt agccaagttc
     4501 ttcagctaat tttgcgtatc cttcacgttc caaacgcgca gccaattctg gaccaccaga
     4561 aaggacaaca cgaccttcca tcatcacgtg taccacatca ggtgtgatat agttcaaaag
     4621 acgttggtag tgagtgatga tcatagcacc aaaaccttca ccacgcatgg cattgacacc
     4681 tttagacaca actttaagag cgtcaatatc aagacctgag tcaatctcgt ccaaaagagc
     4741 aaatgttggc tccaacatca aaagttgaag aatttcattg cgttttttct caccaccaga
     4801 gaagccttcg ttgaggtaac gctctgccat ttcttctttc atgttgagca attccatttt
     4861 ttcatctagc ctagtaataa actcacgaac tgaaatcttc tcatcatctt ctttacccgc
     4921 attcatagcg gcacgaagaa actcagcatt ggtaattcca gggatttctg atgggtattg
     4981 catagcaagg aaaagtccca tacgcgcacg ctcatccact tccaactcaa ggatgtttac
     5041 gccatcaaac aaaacttcac ctttagttac ttcatagttt ggatttccca tgatagcggc
     5101 agaaagagtc gatttacctg taccatttgg tcccatgata gcggcaattt ctcctgtttt
     5161 cagggtcagg ttaacccctt ttaaaatttc ttttccttca atctcaacgt gaagatcttt
     5221 gatctctaat actgacatga tagttccttt ctttttcaaa acttttacag tacttactag
     5281 aaaaacgtct gatttcccca gaaatagagc aaaaattcta taaatatact tttctagtat
     5341 aacaaaaaaa agcctaaaag gctttgattt tgtctggaag ctgagggcta gtctttccta
     5401 ttaaaatgct gatcgatgac atcaacgatt ttgcccatca cataactgac tcggaaattt
     5461 ctaaagagta aaattgccca aaaggctgcc ataatatagc ggccagtcat ccaggcttca
     5521 ttgaaaaaat aggtctcagc agctgtaaac agcgccataa taataaattg ttgtttattc
     5581 ataaacttat tgtatcacga aatctttctt tagtcttcct ctaccttcac tttatcagcc
     5641 aaaaattcaa atccttctgg aatcaggctt tcttctactt ctttttcagt ttgagaagat
     5701 ttggctttgg ctgaaaaggc tgcgcgaact tctttccatt cctccatgga aatagctaaa
     5761 atctcaggtg aaaaacctgc cgcctgactg aggatattgc caaacatggt attgagattg
     5821 tctcgtttca tagtttgacc agcattgaag ttagactcaa aagcaagaat agcatggtgt
     5881 tcattggcag caaccggttg agaaccaact agcagagcct tgtccggccc acctagactt
     5941 tcaattacct ctccccaggc attctgcaaa cgaattagat tttgacgtgc taaatcagga
     6001 ttttcgacgg cctcttgtaa gatagattgc actttattgc gatcgacacg atagactgtt
     6061 ttgcccgtag ctggtcgact aggagctggt gcaacttgtt taggaaccgc acctacatta
     6121 gaaagctctt gtttgagacg ggcaacttcc tgtctcagcg tagcaatttc attttcaacc
     6181 gctcctgata gagctggttc gggcttgatt tccgccaaac ggacggtcat catttcagca
     6241 taaatcttgg gctgcaaact agacttaata tctgctaaac tcactgttgc taagcgaatc
     6301 atttcaaaca gatttttttg aggaagtgcc aaattttcta caaagactga actatgatga
     6361 gtattttctc cccctgtttg aacaattaac aagtctctta aatagtgcaa aagatcggtc
     6421 acaaaacgag tcatgctctt accattgtca aaaagaagat tcaagcaaga caaagctttg
     6481 ggaacatcct gttgagacaa ggccgccaca taatcatcca aggctgatag gctaatagtg
     6541 ccagtaattt cttcagagat agcagtcgtc agctcatttc cctgtgtcaa actcagggct
     6601 tgatccaaaa tagacaaggc gtcccgcatt ccaccttccg cccgtctggc aatgatttcc
     6661 acagcctctg gttcagaact gatattttct ttttctaaga tatagtgaat atgttcctta
     6721 atatcctgtg tcttaattga tttaaactca aaacgttgca cacgggatag aatagtagca
     6781 ggaatcttgt gtaattcagt agtggccaaa ataaagacta cattctgtgt tggttcttcc
     6841 agcgtcttta ggagggcatt aaaagcccct gtagacagca tgtgaacctc atctatgata
     6901 taaaccttat aacgagcaag gctaggcgca taggtagatt tatcacgaat ttcgcgaatt
     6961 tcatctaccc cattattaga agctgcatcc atttcaatga catcttctaa actaccgtcc
     7021 gtcactgctt gacaaatata gcagttattg caaggttcgc cacccacttg attgggacag
     7081 ttcatagcct tggcaaagat tttagcaaca ctggtttttc ccgttccacg aggaccagaa
     7141 aaaagataag cgtgacttat tttctcttgc tccaccgctt gtttaagagt cttagccaca
     7201 acttcttgac caactaactg ggagaagttt tgacttctat attttcgata aagtgcttga
     7261 tacattaaga tttttcctca aacatcgtaa agtcccatgt tgtcttttca agcaaaatag
     7321 cgacaaattg ttccaaataa tctcgatcca tagcatcgta atcctcaatc tctgaagaat
     7381 ccagatccag aactccaagt aactgaccat tcttcatcat cggcaccaca atttcacttt
     7441 tagctagact atcacaagaa atatagttga gataggtcgt cacatctcca acaataacag
     7501 tttcctgaaa gtgagctgcc tcaccacaaa cacccttgcc tagtgcaata cggatgcagg
     7561 aaacacctcc ttggaaggga cctaaaacca attcctttcc atcgaacaaa taaaagcctg
     7621 caaatacggt attaggaaaa cgtgatttta taagagcact ggcgttggaa agattagcca
     7681 aaacattggt ttcgccttcc aataggaagg acaattcttc atttaacatt tgataacgtg
     7741 attgtttttc tgattttaac atagaactat tatatcaaaa aaggagcaga gacaaaagaa
     7801 aaatcccttg tttagtaaac aaaggatttt gtgaattttc aatttgatta aagttcgata
     7861 tcaccaaaca aatcagccat tgaaaatcct gtttgtgttt ctggaagttc gaaatcacgc
     7921 ttttcttgac gtcttggacg acgtggacga gcagcacgtt tttcttcttt ttgtccttct
     7981 tcttgggctg gacgttcttc aagagcttta atagaaagtg acacgcgttc tgcatctgcg
     8041 ttaacttcaa gaactttaac ttgaacttct tgaccaactt taagagcttc ttttggattt
     8101 tcaatccgtt tgtgtgaaat ttgtgatacg tgaacaagtc catcgatacc tggcaatact
     8161 tcaacaaatg caccgaagtc agtcaaacgt ttaactgttc cttctactac atcaccttta
     8221 gccaattttt gctcaacgcc atcccatggt cctggtactg ttgctttaag tgaaagtgat
     8281 acacgtcctt cttcttcgtt aagatcaagg attttcactt caatttcttc accaacagtt
     8341 acaactgatt ttggtgatac gttacgttca tgtgacaatt cagtcaagtg aaccaatccg
     8401 tcaacaccac caaggtcgat gaaagcgccg aagcttgtga tacgagcaac tttaccagtt
     8461 acaacatcac caacagccaa tttaccgaat acttcagcgc gagctgctgc agtagctgct
     8521 tcaacaactt cacgacgtga aaggatgaag cggttttctt tagcgttaac ttctttgatt
     8581 ttagtatcaa attcttgacc tacaaaacgc tcagcgttac gtacgaaacg agtatccaac
     8641 attgaagctg ggataaatcc acgaacacct tcaaattcta ctgaaagtcc acctttaacg
     8701 gcacgcgttc ctttaacagt aacaacttct tcttcgcgac caacaagttt gtcccatgct
     8761 ttgcgagctt caaggcgttt tttagataca aggtatgtaa ctgtatcagt atctttacca
     8821 actacttgac gaagtacaag aacatccaat acttctccta ctttaacaaa gtcattgata
     8881 tctgcatcac gatcgtttgt caattcgcga agagtcaaga caccttcaac accagttcca
     8941 gagattgcaa cgttagcttg agtcgcatca actgtcaata cttcagcact aacaacatca
     9001 ccagtctcaa cttgactaac gctatttagc aaatcttcaa attcgttcat ctaaaaaatc
     9061 ctccaacaat caagtttttc ttaaacttga catacttata atttttttcc taagcacccg
     9121 caaggacatg ttcatatcat tcacgtcttt caattataaa aataaaatac cctaagatat
     9181 tttgcttttt atcttgatta ttacctaaga actggggtag ctggattcga accaacgcat
     9241 gagggagtca aagtcccttg ccttaccgct tggctatacc ccattaatga aatggagaga
     9301 gagggattcg aacccccgaa cccgaaggag cggatttaca gtccgccgcg tttagcctct
     9361 tcgctatctc tcctacaatc aacatggact attatatcat gaaaatttca aaaaaacaag
     9421 acttattttg ctaaattata attttcaatc aaaatttcca cagctttttc caatttttta
     9481 taaaaactat cgacattccc attctttata atggcaaatt gtccgtctaa ttcggcaatt
     9541 tctccgataa cttttttacc gatagtcaat gtataacctt caaaactgtc tttaccaaca
     9601 ttaactttgg catccgctac ttgaatttcg atttttttat ctttcttgct catttcatac
     9661 ctctcttcac tttttctatt ttacaagaaa atcctaaaac tagcaagaaa aaactctata
     9721 tcaggcttct ctgatataga gttttatgta aacaacctca attgatttag catagttaat
     9781 agttagtaaa caagcataat tgactatcct ttttaggtta tcagtgagtc tcaccgccta
     9841 ctaactgcaa atccttatct ttgtagtcta caatagcttc aatagctacc tctatgcctc
     9901 ttacaatatc acatagactc attcctgcag tattcggctt atttactact tgttccatca
     9961 tatatggaat atgcatgaaa cctgctctca tattagggaa ttttttatcc actaaataaa
    10021 gagcttggta catcaaatga ttgcaaacaa aggttcctgc actattggat acaactgccg
    10081 gaagtccctg ttttttgata gcttgtacca tcgctttgat aggtaaacta ctaaaatagg
    10141 ccgatgctcc atcaatacga atcggtgtat caattggttg attgccttcg ttatcaggta
    10201 tgcgagcatc atcttgatta atagccactc gttcaggtgt taagccggtc ctgccgcctg
    10261 cttgtccaat acaaagtaca gcatctggtt gatatcgtaa tatttctgcc tctaaaactt
    10321 ctgacgactt ataaaaaacc gttggaattt ctacccagcg aacttcagcc ccattaatct
    10381 cagatggtaa taattttaca gcctccaaag ctggattaat cttttcacct ccaaaaggat
    10441 taaaacctgt aaccaatatt ttcattttat tttcctttac taaaatgcga gaaagtacat
    10501 taagaatatg tgaataacaa tcattactag agcaacacct gcttgagcct ttataacgcc
    10561 attctgatct ttcatatcca tcaatgctgc tggtagagcg ttaaaattag cagccattgg
    10621 ggtcaataag gtcccacaat aacctgctgt catggcaaga gcaccagcca caattggatt
    10681 agctcccaga gcaaatacaa agggaactcc aacacctgct gtaataacgg tgaatgctgc
    10741 aaaagcattt cccataatca ttgtgaatag aaccattcca agaacataag ccaaaactcc
    10801 tataaagcga ctatctgaag gaacaatacc gctaatcaga tgagagataa catcaccaac
    10861 acctgctaca gtaaaaatag cccccaaagc ccctaataat tgaggaacaa tcccacttgt
    10921 tgaaacttgc tgagtcattc gattattttc tgataacaga ctcttagggt gactattggt
    10981 aatcacaaga acagaaattg tagcaaacaa ggcggcaagg ctaatcgaaa tcttgctaaa
    11041 ttctggaatc atttgcgcta agaccaacgc aagtattgcc atcagcataa ctggaataaa
    11101 aattttattt ttcaacctgt tagattcaat attggctttc atttcatcta aggatggcaa
    11161 ggttccgata cggacttgct taaacaatgt taacagcgat aataggatta caataatacc
    11221 aatactcata tttggcatat aggaaccacc tataaacgta atagacaata gagtccaaaa
    11281 tgcagatgtc ccaagtcgaa ctgggtttgt tttatcttta taactacaat aggctgtatg
    11341 gagaaattga caaccaatca caatataggt caactctaat agttgctttg ccaactctgt
    11401 catttttgtt ctcctcccct agtctttttt gatatcaatt ttttatcaaa taaataattg
    11461 taaatcccca ctacaataag tgttataaca gcaacaataa tagatgtaga agcaatccct
    11521 gcataattgc tttcatagcc taactgatct aatgttcccc ctatcaagag gactccccca
    11581 gcacctacaa acgtattttg agcaaagaaa tttccaaaat tttcattcgc agccgcacgc
    11641 gcttttattg tctcatcttc aacctctgtt aactttctac ctaattgaga ctctgcagct
    11701 gcttctccca taggttgaac caaaggtctg acaaactgag ggtgtcctcc tagacgaatt
    11761 gaaaagaaac cagctaactc tcgaataaag aaataaactg tatagaagtt tccaactgtc
    11821 agacctttaa tctttcgaat caaatcgatt gatcgttgct tgagtccaaa ggtttctgac
    11881 agccccacaa gaggcaaggt aaccataaaa atcgtgagca ctcgctgatt gctaaattct
    11941 tttcccaaaa tctccaaaaa ttcaacgaga gaaacacctg aaactaaagc tgtaaccaaa
    12001 ccagctaaga ctactgttgc aattgtatca aattttaaaa taaaacccac aacaatgatt
    12061 gctattccta ttaatctaat ccactccata tcaaactcct ttatattcaa aatgacagta
    12121 ttttaaaaat tttatcaaga tcaataccat tccttattta atgtattttt ctagttcttt
    12181 ttggtatttg ctattggatt ccaatttttc tttttgccat tttttaaaaa cctcgttata
    12241 ttcttttgtt gtaacaatat ctttttgcaa tttcattcct ttaaagatat atggttcccc
    12301 cttaatacca acttgtgagt atggttttga gaatggtact acgttactta caactggaaa
    12361 accaccagat gaagctgttg gcatcaataa tgaactatct gtcgaccaag cttgagcttt
    12421 ggcatatttt tcatatctct tctctaggtc agtggtctca gaaacagcat cttctaacaa
    12481 tttcttatat ttatccaaac caggtttagc tacaacatcc ttatcttttc ctttcgtaat
    12541 gccaaggtgt ttcatgtcag aaccagattt tggatccata atattcaagt aagacgctgg
    12601 atcttgatag cttggagccc atcctgtact gttcaaatca tagtcttttt gagaaggagc
    12661 aacattgccg tatttatcat tttccatcaa accatcaata acatttccaa taacgtctgt
    12721 cctcgatgtt cgagtcgcta tactgtagcc caatgatgct ggatctactg catagacata
    12781 agaaaatgtt gtcggtgcat ctgcttcttt atcagttttt ccacaagcca ctaaaatagc
    12841 tgacgtgctc aggaccactc ctgctgttaa gagccacttt ttctatttca taaaaaatct
    12901 cctttggttt attttaatct acttttacaa tccaaccttc tggcgcttca atatcgccaa
    12961 actgaatacc cgtcaattca ttatataatt tacgcgtcac aggacctact tctgtttcac
    13021 tatagaatac atggaaatca tcaccatgtt gaatacctcc aattggagaa ataaccgctg
    13081 ctgtaccaca ggcacctgcc tctacaaaac ggtcaagatt atcaattgga acatcaccct
    13141 caataggagt taatcccaag cgatgttctg ccaaataaag caaggaatac ttggtaatag
    13201 atggcaagat agatggactc aatggtgtta caaattcatt atcagctgta attccaaaga
    13261 agttagctga tccgacttct tcaatctttg tatgagttga tgggtccaga tagataacat
    13321 ctgagaaatg acgtgacttg gccatttttc ctggtaagag acttgcagca tagtttccac
    13381 caaccttagc cgcacctgta ccatttggtg ctgcacggtc gtactcatcc tgaatcaaga
    13441 agttggttgg gaccaaacca cctttaaagt aatttccaac tggcatagca aagatggtga
    13501 aaatgtactc ttctgccggt tttaccccga taatatctcc gacaccaatc aaaagagggc
    13561 gaagatataa ggttccacct gttccgtatg gtggtacgta ttcttcattc gcacggacaa
    13621 ctgctttaca agcttctaca aacatgtctg tcggaacttg tggcatcaag agacggtcac
    13681 atgtacgttg cagacgttta gcattttcat caggacggaa cagttgaaca ctgccatcct
    13741 tagtacgata agctttcaaa ccttcaaatg cttgttgtcc atagtgaaga cttggagaag
    13801 actctgaaat atgcaaagtt gcatcctctg taagctctcc ttgatcccat tgtccatttt
    13861 tgaaatgagc aagatagcga taaggtaatt tcatatagga aaaaccgagg ttttcccaat
    13921 caatcgttac tgtcatattc cactccttat tctaaaaacc tatttcttat attctacact
    13981 atttttctaa aatagcaagt atattttgta attttcagaa aatttctcca ataaaaacca
    14041 gctcttagaa ctgattcttc atttcactta tttatcttca gtaactactt cctgaagata
    14101 agcgtcaaaa acttcttcat ctgaaatcgt gtcagaaatg aagcttccat tgctagtgcg
    14161 ttctgacaag ttcaagtctt gcaatcggct ttcatagatt gttcctttat tggattggac
    14221 aagcagagtt tggtcgttca catccacttc cgtactgaag aaatcgccaa caaatccttg
    14281 ctctgcaact gctcctgcca agaagacacg atgcggtttg tttttcaact cacgcaagac
    14341 ttgtaatcct cgtttggcac ggctggttgc tagaatttcc tcaatagaaa cacgtttcaa
    14401 gcttccacgc tgggtcaaga ggtagaagga cgaagtatta cagataaagc cagattggag
    14461 gacatcatct tctttcaaat tcatagcctt gacacctgct gccttagcac cgacaaccgg
    14521 aacctcttcg atattgaaac gcagggcata accattttga ctaaccaaga caacatcatc
    14581 tagtttaatc ggagccactg ctacaatctg atctgtatcg tctttgagct tagcatactt
    14641 gacagactta gatctatagg tccgccatgg agtgaattct tttcgctcta cccgtttgat
    14701 ttgaccaagg cgagtcactg caaagtaggt tgtcgcatcg tcaaactgat ccagtacttc
    14761 cacataaagg atttcttcat tcgtttcaaa gtttgtgatg gtttggctca gatgctctcc
    14821 gatgtccttc caacgaatat ctgccaactc atggattggt ctgtagatga catttccaag
    14881 acttgtgaac atcaagaggt gctgggttgt cttggcagat tgaacaaaaa tcaaacggtc
    14941 atcatcacgc ttgccaattt cttccaaggt ggaagccgca aaggaacgtg gactggtacg
    15001 cttgatgtaa cctgccttgg tcacgctgac gtaggtatct tcctcagcga taagactagc
    15061 tgtatcaatc tcaattgctt tcgcagtgtc ttctaaagaa ctcaaacgag gagttgcaaa
    15121 tttcttcttg acctcacgaa gttctttctt catgagattg tacatagtcc tttcatcacc
    15181 gataatagcc gccagcatag caatcttctc acgaagctct gcttcttctt cctgcaagac
    15241 aaccacatcg gtattggtca aacggtacag ttgcaaagtt acgatagcct cagcctgttc
    15301 ttccgtaaaa tcatagctaa ctttgaggtt ttccttggcg tccgccttat tctcagaagc
    15361 acggataaga gcaatgactt catccaaaat cgaaatcaca cgaatcaaac cttcgacgat
    15421 atggagacgt ttctcagcct tttctttgtc aaagcgtgaa cgcgccaaaa tcacttctcg
    15481 acggtgagcg atgtagctag acaggattgg aacaatccca acctgacgag gtgtgaaatt
    15541 gtcaatcgcc accatattaa agttgtagtt gatttgtagg tcggtgtact taaataagta
    15601 gttgagaaca agctcagtat tagcgtcttt cttaagttcg atagcgatac gaagaccatc
    15661 acggtcagac tcatcacgaa cctcagcaat cccagctacc ttgttattaa cacgaacatc
    15721 atcgattttc ttgactagat tggccttatt gatttcataa ggaatctcag taataacgat
    15781 ttgttcctta ccacctttta gcttttcaat ttcagtcttg gaacgaacaa ccacgcgccc
    15841 tttcccagtc tcataagctt tcttgatttc atcacgaccc tgaataatag cccctgtagg
    15901 gaagtctggt ccaggcaaga attccatgag tttatcaatc tttgcagttg ggtggtcaat
    15961 catgtaaact gcagcatcta tgacctcagc taaattatgg ggaggaatgt ctgtggcata
    16021 accagccgaa atcccagtcg aaccattgac caagaggttt ggaaaggctg ctggcaagac
    16081 cgttggttct ttctccgtat cgtcaaagtt ccatgcaaaa ggaactgtat ttttctcgat
    16141 atcctgaaga aggtaacctg caatttcaga caaacgtgcc tcagtataac gcatagccgc
    16201 aggaggatct ccgtccatag aaccgttatt accgtgcatt tcaactagaa tctcacgatt
    16261 tttccagttc tgtgacatac gaaccatggc atcatagata gaagaatccc cgtgtgggtg
    16321 gaaattcccc atgatgttcc cgactgactt ggccgactta cggtagctct tgtcaaaagt
    16381 attgctatcc ttattcatag aataaagaat acggcgctga accggcttca acccatcacg
    16441 aatatctggc aaagcccggt cttgaataat gtacttggag tagcgaccaa agcgctctcc
    16501 catgatgtcc tccagggaca tgttttgaat gttagacata agatacaaag cccataaaat
    16561 accaagtgaa aatagaaaat tcttgaagta agcaaactca caagagaatt tatctttttc
    16621 acacagtatc tagggcgtgt tcaactcctt tcaaagaatg tagagtaggt ttttatgcag
    16681 taaaagatat tttacgggaa ttcctcccgt gttcagttac gataagtaac caaactatcc
    16741 tgtttgtatt tttcaatatg aaaatctggt tttccaaaat tagtcttagt ttgtgtctta
    16801 gccgctccct taagcgcctc tttgagataa gcactcatag cagattcttc attaataatc
    16861 ctgcaatttt ttcaaaccaa gattttcaaa ctgctttttc acatagtcat tcacatccga
    16921 ctctaatttc cagtttacta acatattatt ttctttcatt aaaacactgt cgcttcttct
    16981 agcgtaaact tgacattatc ttcaatccat ttacggcgtg gttctacctt atctcccatg
    17041 agaacattga cgcggcgttc ggcgcgcgct aaatcttcaa ttgtgacacg gatgagggta
    17101 cgtgtttctg ggttcatggt tgtttcccag agctggtccg cattcatctc accaagtcct
    17161 ttgtatcgtt ggagggtagc gcctttaccg aactgtttac ggagttcttc tagttctccg
    17221 tccgtccaag cgtaggccac ttcttctttc tttcctttac ctttggacat cttgtaaaga
    17281 ggtgggaggg caatatagac atgacctgcc tcgactagcg gacgcatgta acggtagaaa
    17341 aatgtcaaga gcaaggtctg gatatgggca ccgtcggtat ccgcatcggt catgataatg
    17401 atcttatcat agttggcatc ttcaatagag aagtctgctc caacacccgc accaatggta
    17461 taaatcatgg tattgatctc ttcatttttg aggatatccg ccatcttggc cttggctgta
    17521 ttgataacct taccacgaag aggtagaata gcctggaact tgcggtcacg accttgtttg
    17581 gcagaaccac cggcagagtc cccctcaact agatagagtt cattcttagc aggattctta
    17641 gattgggctg gggtcaattt cccagacaac aagcccttat ctttcttgtt tttcttccca
    17701 tttcggctct catcacgcgc cttacgtgct gcttcacgag catcacgggc cttgatagcc
    17761 ttgcggatga ggttagaagc taattcccca ttttccataa ggaaaaaggt caacttatca
    17821 gccactattc catccacaac tgggcgagct agggggcttc ctagtttatc cttggtctgt
    17881 ccttcaaact gcaagtgttc ttcaggaact aagatagaaa gaacggccgc tagtccctca
    17941 cgatagtctg aaccttcaag gtttttatct ttttccttga gaagacctgt tttacgtgca
    18001 tagtcattca tgaccttggt aatggcagac ttgagtcctg tctcgtgcgt tccaccgtcc
    18061 ttggtgcgaa cgttattgac aaaggataga atgttatctg agaatccgtc attgtactgg
    18121 agggctactt ccacttgaaa accattgtct tccccttcaa agtaaagaac tggcgtcaag
    18181 atttccttat cttcgttgag ataagaaaca aaatcttgta ctccattctc atagtggaac
    18241 tcaatcgctt catctgttcg cttgtccgtt aaagacaagg tcacattttt caagagaaag
    18301 gctgattcat taaggcgctc tgaaatggta ttgtacttga aatctgtcgt agaaaatata
    18361 gtcgcgtcag gcataaaagt aactttggtg cctgttttag acttgggtgc tgtaccgatt
    18421 ttcttcaaag tcgtgacagg ttttccacca ttttcgaaac gttgcttgta aactgcgcca
    18481 tcacgggtaa tttcaacttc taaccagcta gaaagggcgt taacaacgga agaacccact
    18541 ccgtgaagtc cacctgatgt cttatagcca ccttgaccga atttccctcc ggcatgaaga
    18601 atggtaaaga taacctcaac agttggaatt cccatagcgt gcatacctgt cggcatccca
    18661 cgtccatggt cttgaaccgt tagactaccg tctttattga tggttacatc gatacgatca
    18721 ccaaacccag acaaggcttc atcgactgca ttatcaacga tttcccaaac taggtgatga
    18781 agaccagcgc catcggtcga tccaatatac atccctggac gttttcggac cgcatccaac
    18841 ccttctagca cctgaatagc atcatcatta taattgttaa tattgatttc cttttttgac
    18901 acaaggaacc tcctattcgt tcatctttac tattctacag gttttccaag gattttgcaa
    18961 aatttttctt tctccgatgt gacaatttta gcagagattc tctgcttttc tttcccaatt
    19021 catgatataa taggagtatg attacaatag ttttattaat cctagcctat ctgctgggtt
    19081 cgattccatc tggtctctgg attggacaag tattctttca aatcaatcta cgcgagcatg
    19141 gttctggtaa cactggaacg accaatacct tccgcatttt aggtaagaaa gctggtatgg
    19201 caacctttgt gattgacttt ttcaaaggaa ccctagcaac gctgcttccg attatttttc
    19261 atctacaagg cgtttctcct ctcatctttg gacttttggc tgttatcggc cataccttcc
    19321 ctatctttgc aggatttaaa ggtggtaagg ctgtcgcaac cagtgctgga gtaattttcg
    19381 gatttgcgcc tatcttctgt ctctaccttg cgattatctt ctttggagct ctctatcttg
    19441 gcagtatgat ttcactgtct agtgtcacag catcgatcgc ggctgttatc ggggttctgc
    19501 tctttccact ttttggtttt atcctgagta actatgactc tctcttcatc gctattatct
    19561 tagcacttgc tagtttgatt atcattcgtc ataaggacaa tatagctcgt atcaaaaata
    19621 aaactgaaaa tttggtccct tggggattga acctaaccca tcaagatcct aaaaaataaa
    19681 atgccagttc tgtgaactgc accccaaaag ttagacaatt aatttatccg aaggatttag
    19741 ttctgtattg cacagggcta agtcctttta gtttta
//
LOCUS       DAVBCN010000035        19556 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVBCN010000035 DAVBCN010000000
VERSION     DAVBCN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19556
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.035"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(<1..777)
                     /locus_tag="VQ497_001153"
     CDS             complement(<1..777)
                     /locus_tag="VQ497_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2517465.1"
                     /translation="MSKETVVYTKRREKIKDNILGYSFLAPALILLGIFLVIPVGMVI
                     YYAFTDYYLLTPDERKFVGLENFIRLTKDPIFLKSFLNTLKFVVWIIPVQLGAALGMA
                     LIVNKKRKGNMFFKVAFFAPVVMSLVVISILWLYLLNPNSGLLNAILNKVGIASQPFL
                     TSPKQAMYAIVFVSAWQGAGYQMLLFLGGMQNIPQDVYEAAELDGFSKWAQFRYITMP
                     LLKPTALFVLLTTLISAFKLIVQPMVMTQGGPLNSTITMVY"
     gene            complement(873..2183)
                     /locus_tag="VQ497_001154"
     CDS             complement(873..2183)
                     /locus_tag="VQ497_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET2517466.1"
                     /translation="MRKGTMLSVVAGLSLAILAGCSGGTNSKQASSSNDIKVWVQFSD
                     ETNEGKAWQKVVDNFNQSGKSKYKVVTEYIPRSGSGGGYEDKVNAAITTNSLPDVITL
                     DGPNTAAYAKSKVITPLDDYLKDNNMDDVLDSIKQQGTYNGKFYAFGFSESNVGVYYN
                     KKMFKEAGIAESELPTLEKPWTWDEFNTIAKKLKDHYNKPAIDFRINSNDEMLPYAYM
                     PLIWSNNGSVVNEDGTKAEGYFNSKESVEAVQFIQNLVKEGYTTVSPVEKGFETGEYP
                     MLLSGSWTIADMNTNYKDIDFGILPYPVSSKTKKLVSPSGSWQLAVTTKSDKKEAASE
                     FVKFATNTESSEIISLGNSVLPIRKSTIENIKDKVSEPMRFLMEQNSKTAHARPVVVA
                     YPQVSRAFQQAMQDISYYEENPNVQKVLDTRTKEMQTAIDQSLK"
     gene            complement(2310..3305)
                     /locus_tag="VQ497_001155"
     CDS             complement(2310..3305)
                     /locus_tag="VQ497_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2517467.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLRKVQR
                     AIKTLDYIPDNYARGMKKNRTETIALIIPSIWHPFFSEFAYHVEKELLKYNYKLFICN
                     ADSDSQNEIEYIEMLKQNKVDGIIGITYSDIDKYILSDLPFVSIDRHFSENVCYVTSA
                     NYKGGQTAAEELVRRDNKHLAFIGSINMYENETILRSKGFREKAKELGVDVYNFEMKE
                     PLINPEDQIRIFLQSHKEIDGIFTINDMMALRVISVMKELGKIAPDDYQIIGFDGLKT
                     SVDQSYLVSTIVQDIGMMAKESVAMILKMIKREEILEKRIVIPTYFGGDKTTKKI"
     gene            complement(3667..5148)
                     /locus_tag="VQ497_001156"
     CDS             complement(3667..5148)
                     /locus_tag="VQ497_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2517468.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSS
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            5591..5755
                     /locus_tag="VQ497_001157"
     CDS             5591..5755
                     /locus_tag="VQ497_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET2517469.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            5839..6405
                     /gene="amaP"
                     /locus_tag="VQ497_001158"
     CDS             5839..6405
                     /gene="amaP"
                     /locus_tag="VQ497_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HET2517470.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            6417..6587
                     /locus_tag="VQ497_001159"
     CDS             6417..6587
                     /locus_tag="VQ497_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HET2517471.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            6618..7226
                     /locus_tag="VQ497_001160"
     CDS             6618..7226
                     /locus_tag="VQ497_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2517472.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            7257..7460
                     /locus_tag="VQ497_001161"
     CDS             7257..7460
                     /locus_tag="VQ497_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET2517473.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(7569..7847)
                     /locus_tag="VQ497_001162"
                     /pseudo
     CDS             complement(7569..7847)
                     /locus_tag="VQ497_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8045..8332
                     /locus_tag="VQ497_001163"
     CDS             8045..8332
                     /locus_tag="VQ497_001163"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2517474.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCSRQRRLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(8610..9380)
                     /locus_tag="VQ497_001164"
     CDS             complement(8610..9380)
                     /locus_tag="VQ497_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET2517475.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(9395..10243)
                     /locus_tag="VQ497_001165"
     CDS             complement(9395..10243)
                     /locus_tag="VQ497_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HET2517476.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDRFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(10240..11715)
                     /locus_tag="VQ497_001166"
     CDS             complement(10240..11715)
                     /locus_tag="VQ497_001166"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HET2517477.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYVSDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTSLKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(12038..13393)
                     /locus_tag="VQ497_001167"
     CDS             complement(12038..13393)
                     /locus_tag="VQ497_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2517478.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(13403..13720)
                     /locus_tag="VQ497_001168"
     CDS             complement(13403..13720)
                     /locus_tag="VQ497_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2517479.1"
                     /translation="MDESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(13737..14054)
                     /locus_tag="VQ497_001169"
     CDS             complement(13737..14054)
                     /locus_tag="VQ497_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2517480.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(14067..15752)
                     /locus_tag="VQ497_001170"
     CDS             complement(14067..15752)
                     /locus_tag="VQ497_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HET2517481.1"
                     /translation="MKNKKLISSLFILFLLIFIHVKVFANENLIKNSNFEQGELFWNS
                     RNRGIINSNESYSNGHYSGMIPSTAINDNRADGYIGQVIDIEPNEDYLVSAFAKVDIS
                     GAEGYFTARWFDNNKQGEIVQNNIGEAVDQTVNTTEWKKYMFSFNSGKHDKVLIQLVK
                     WSEDDTTKKSNIFIDNVEMYQLSKGNSYKKIWRDDFDGEQLNKKYWGYELGSIRGWEQ
                     QHYVRSDENVFLRSGNLVLRATNRSKEDQYFNPRNNHRKVVYNSGSVRTHGKVEFLYG
                     KLEMRAKLPKGQGVFPAFWTLGSDFTLDGKINPVQGRGWPSTGEIDIMELVGERNSNG
                     SGNKTVYQTLHYGQSDNDDGKFAGRGTAFKLSSGNFNDSYHTFSIDWYKDYIVWMVDN
                     HIVRKVYYGSDNIAKKIFNRPQYVQLNLAMGGSWPGVVDNNLSGTEFVVDYVSYSRND
                     LQQHQAEEYYSQSPKINGAKDIVINRGDVPDLLKGITTNSGYELDYSIENEPMFNNFG
                     GNTSVDLLIRNKTEKEKISQMPPGVYNLHYTSRPKHLDYESKVDRKTVTLTIK"
     gene            complement(15757..16653)
                     /locus_tag="VQ497_001171"
     CDS             complement(15757..16653)
                     /locus_tag="VQ497_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HET2517482.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(16656..17918)
                     /locus_tag="VQ497_001172"
     CDS             complement(16656..17918)
                     /locus_tag="VQ497_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2517483.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDILLYLPFVIAIEKRFKLLED"
     gene            18198..19376
                     /locus_tag="VQ497_001173"
     CDS             18198..19376
                     /locus_tag="VQ497_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET2517484.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALFYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT         6658 a         3779 c         2827 g         6292 t
ORIGIN      
        1 gtacaccata gtaattgttg agtttaatgg gccaccttgc gtcataacca ttggttgtac
       61 aatcagctta aaagcagaaa ttaaagttgt taacaataca aacagggctg ttggttttag
      121 taggggcatc gtgatataac gaaactgagc ccattttgag aatccatcta attcagcagc
      181 ttcatagaca tcttgtggaa tattctgcat tcctcctaaa aataaaagca tctgataacc
      241 tgcaccctgc caagcagaaa caaaaacaat tgcatacata gcttgtttag gactagtcaa
      301 gaaaggttga gaagcaatac ctaccttatt taaaatagca tttaataagc cactatttgg
      361 atttaaaaga taaagccaaa gaatagaaat aactaccaag gacatgacaa ctggagcaaa
      421 aaaagccact ttaaaaaaca tatttccttt tcgcttttta ttgacaatca aagccattcc
      481 taaggccgct cccaattgaa ctggaataat ccaaacaaca aatttcaaag tattcaagaa
      541 gctctttaag aaaatcggat cttttgttaa tcgaataaaa ttttcaagtc caacaaattt
      601 tctttcatct ggtgtcaaca aataataatc tgtaaaagcg taataaatga ccattccaac
      661 tggaattact aaaaagatac ctaataaaat taaagctgga gctaaaaaac tataacctaa
      721 tatgttatcc tttatttttt cacgtctttt tgtatacaca acagtttctt tactcatatc
      781 catctccttt aatcatatat atcaaactag gctattaaca agaaagggga aggtagttcc
      841 aaacattttc cttccacctt cttttttaca gattatttga gtgactgatc aatagcagtt
      901 tgcatttctt ttgtacgagt atccaagact ttttgcacat ttggattttc ttcataatag
      961 ctaatatctt gcattgcttg ttgaaaagct cgagaaacct gaggatatgc tacgacaact
     1021 gggcgagcat gagctgtttt tgaattttgt tccatcaaga aacgcattgg ttcggatact
     1081 ttatctttga tattttcaat tgtagattta cgaattggaa ggaccgaatt ccccaagcta
     1141 ataatttcac ttgattctgt atttgtagca aatttaacaa attcagatgc ggcctctttt
     1201 ttatcagatt ttgtcgtcac agctaattgc caactacctg aaggggatac taattttttc
     1261 gtcttactag atactgggta aggaaggata ccaaaatcaa tatctttgta gtttgtattc
     1321 atgtcagcaa tcgtccaaga accactcaaa agcattggat actctcctgt ttcaaaacct
     1381 ttttcgactg gactaacagt tgtatagcct tcttttacaa gattttgaat gaattgaact
     1441 gcttctacac tctctttgga gttgaagtat ccttctgcct ttgttccatc ttcgtttacg
     1501 acagaaccat tatttgacca aatcaacggc atataagcat atggcaacat ttcatcgttt
     1561 gaattaatac gaaaatcaat tgctggtttg ttataatgat ctttcaattt tttagcaatc
     1621 gtattaaatt cgtcccatgt ccatggcttt tctagcgttg gaagctctga ttccgcaatt
     1681 ccagcctctt tgaacatttt tttattgtaa taaacaccaa cattcgattc agaaaatcca
     1741 aatgcataga atttcccatt ataagttccc tgttgtttaa tactgtccaa aacatcatcc
     1801 atattattat cttttagata atcatctagc ggagtaatta ctttagattt tgcataagct
     1861 gccgtatttg gtccatcaag ggtaataaca tctggaagac tattagtcgt aatagcagca
     1921 ttaaccttgt cttcatatcc tccaccacta ccactacgag gaatatactc agttacaacc
     1981 ttgtacttac tttttcctga ttgattaaag ttatcaacaa ctttttgcca agcttttcct
     2041 tcatttgttt cgtcagagaa ttgtacccag actttaatat catttgaaga actcgcttgt
     2101 tttgagttag ttcctccact acaacctgca agtatagcta acgatagccc cgcaacaaca
     2161 gataacattg tacctttacg catcattttt ctcctttttg aaacgtttca ttttttattt
     2221 attttttaaa caagcttcta gcctattttt gaaacgtttc atttgttgat tatattataa
     2281 cagcgctttc attaattgtc aagcgctttt taaatttttt ttgttgtttt atctccccca
     2341 aaatatgtgg gaattacgat tcgtttttct agaatctctt ctcgcttaat cattttcagt
     2401 atcattgcaa cagattcttt tgccatcata ccaatatctt gaactattgt agacactaga
     2461 taagattgat cgacagaagt ttttaaacca tcaaacccaa ttatctggta atcatcaggt
     2521 gctattttcc ccagttcttt cataacacta ataactctca gagccatcat atcatttatt
     2581 gtaaatatac catcaatttc tttatgagat tgcaaaaaaa ttcttatctg atcttcagga
     2641 tttattaaag gttctttcat ttcaaaatta tagacatcta ctcctaattc cttagctttt
     2701 tctctgaatc ctttacttct gagtatagtc tcattctcat acatattaat tgaaccaata
     2761 aaagcaaggt gcttattatc ccttctaacc agttcctctg ctgcagtttg tcctcctttg
     2821 taatttgctg aagtaacata gcatacattt tccgaaaaat gtctatctat acttacaaac
     2881 ggtaaatcag ataaaatata tttgtcaata tcagaatatg taataccaat aattccatcc
     2941 actttatttt gtttcaacat ttcgatatat tcgatttcat tttgtgaatc gctatcagcg
     3001 ttacaaataa ataatttata attatatttt aataactctt tctcaacatg ataggcaaac
     3061 tccgaaaaaa atggatgcca tatgctcgga ataatcaaag cgatagtttc tgttctattc
     3121 tttttcatac ctcgagcata attatcaggg atatagtcca atgtttttat agccctttga
     3181 actttcctta aagttacttc tttaatgcct ttttctttat taattacacg tgaaacagtt
     3241 ccaacactaa ctcctgcttc taaagcaaca tctttcatgg taattgattt tctttgttct
     3301 accatattat cacctccttt caatatatag tatcatgcaa atgcttttta agcaactatt
     3361 tctcaatcat ttttggccag atcatttatc ccatcatgaa taaaatcact ccaattagct
     3421 tttgaaaata cttcaatttt catgtgtaaa catctacata aaacaggaaa agccttggtt
     3481 ttatggcttt tttcgtatct tctacaaaaa aagcaagagt tttagagggc tataaatcta
     3541 gatgtacatt ttgcttaaat gattgaaggt cttttcttaa caaaaacacc ccaaaaatta
     3601 gattttttct gtctaacttt tgaggtacag ttcaaacgcg aaatagcgtt tttttgttat
     3661 ttttggttac tcatctaatc gaataaacat catggcattt aacaaatata tgagtgagac
     3721 cgtgtttata ttatttgaat agatgagtct cttattttca ataggaggaa taataaaatt
     3781 agaaataatg atatcataag atgaatcttc taaagattcc tttgataatt ctaattcagt
     3841 ccaaacttcc agttcaaaat tattgctaca gtaataagaa agtgtctctg caacgaattt
     3901 tgcatgatac tgatcaaaat tactcataac caaaaccttt agtttaggct gattttgtag
     3961 caaattaatc accaaatgtt tggtatgagt gatgaaggta taagatagat gatttaccat
     4021 cattgaacta gaacaaacct caagagtctc taaatagtga gaaagctctt tttttatatc
     4081 tgaaacaaat tttggaaaaa tattttgaaa gttcctgatt gtattccctt tttgatcaaa
     4141 taaaataaac tcagtaaaca actcttgacg atacagatgt gcggtattat gcagatgcca
     4201 aatcagatta tccttattct ccatttcaat ctgatacttg actgaaatct gatcaataaa
     4261 atcactcaat agatggtaag atttttcaac ataactatcc ttttttacgc atttcataaa
     4321 gagactttca tctatgaaaa acattttttg aaagtaagac acaaataatt ggcaaacaac
     4381 ttcttcatct aaagagatat tgtattctga ttcaaaactc tgagcaacac cttctattcc
     4441 ttctgcctgc attaaaaaat ccaaactttg gtcgttaaaa gaatctttat ctacttccat
     4501 aaaatgacca aactttattc tatataggtt cgtaactagg agcaacttta gcattctatg
     4561 cgttgacaaa ttcattggaa agcttgtttc cttataaacc aattctaaca attgagatag
     4621 tggctctgat gaaaaatttt caaatggcca ttctaggaaa taatattttt ctgaaaaata
     4681 ttgtgcaaaa aagtaacgaa tgtctctctc atttccaatg atttgaacag gggtcagact
     4741 gacttcaaat tgaaattgcc ttttaatcac tttattgatt tggctaataa tacgatagag
     4801 cgaagatgaa ctgatataaa attctttaca aatactctca gcttgacaac cttcattaaa
     4861 gaagatgaat tctaaaatcg aaaaatgagt tgaatgttta aagaaatgat ggtaaaccat
     4921 ttcaatatca ctatcatcgg tattaataat gcgtatacca ttagtagaag aatgaaaaat
     4981 caagtcagga aaagcagatt taacatggga tagatcatct ttgactgcac gttctgtaca
     5041 atttaataac tctgctagtt cagaacgatg aaaccaacgt ttatgttcaa ataataattc
     5101 taataattct aattgcctat gacttttttt agataataaa tctctcatga atatctttct
     5161 ctctttataa attatcggat taaacctctt gcaattatac cacaaagaat aggtatagca
     5221 tgatataacg acttttccta aaatctttta tttcgtataa taacactacg gagacaatat
     5281 ataaacaatt ttcttatttt accgtctatt gagggcgtga atacagaatc aaattcaagt
     5341 ctaaagatta tatttttaat tttaaaaatt atataatagc aacaattaaa gaatttgatt
     5401 ttttaaaatt atataataat aacaatcgaa ataattgact tttctatatt aaagttatat
     5461 aatagtatta atcaaagaaa ttgatttttt gatattaaaa taaaaaagga gggtaggcag
     5521 tgttgtgatc aattattgct ggaggtctta ttggtctctt ggcaggtaaa atcactaaaa
     5581 aagtagttct atgggaatca tcgcaaatgt attcgctggt ttagtcgggg catatgcagg
     5641 acaatctctt ttaggtagtt ggggtccatc aatcgctgga atggctttgc tcccatctat
     5701 tgtaggtgca gcgattgtga ttactgtagt gtcattcttt acaggtagaa agtaaacttt
     5761 tcgccagtaa agttagcaaa ctatttttaa atcaatgacg ggaaaaatag tttaaatgtt
     5821 aaatcgaaag gattgtatat gtcaaaagca aagaaaatat gtttcattat tttctgtatt
     5881 ttaatcttga caattttcct tcctgttttg atagattatc atcaagttag tgatctaggt
     5941 attcatctac ttagctggag acagaactcc gtagttgaat tctatcttgc tagatatgtc
     6001 ttttggggga cagtggttct atcaacttta gttttattat ccattttagt tgtgatgttt
     6061 tatcctaaac gttacttgga aatccaactt gaaactaaaa acgatacatt aaaattaaag
     6121 aattcggcaa tcgaaggttt tgttagaagt ttggtgagtg atcatagatt gatcaagaac
     6181 ccaactgttc atgtaaattt acgaaaaaat aaatgtttcg ttcatgtaga aggtaaaatt
     6241 cttccttcag acaacatcgc tgacagatgc caaataattc aaaatgaaat aactaatgga
     6301 ttgaagcagt tttttggtat tgagcgtcaa gtaaaacttg aagttgcagt aaaaaattac
     6361 caaccaaaac ctcaaaacaa aaagactgtt agtcgtgtga agtaaggaag taaaaaatgg
     6421 aatggcttaa acaatatcga tatccaatta tcgctggtct cataggcgta tttctggctt
     6481 gtttgattgt ctcctttggc ttcttcaaaa caatatttgt attgatttta ggagcactgg
     6541 gagttgcagc tggattatat atcgaaaaaa actatataga taaataaaaa ttactaattt
     6601 aattaaagga gtttcatatg tcaaacgaaa aaaacacaaa tactaacgta gaaaagaaag
     6661 atgctactgt tgtagctcac gaaatcaaag gggaacttac ttacgaagat aaagttatcc
     6721 aaaaaatcat tggtctttca ctagaaaacg tttcaggtct tttgggaatc gatggtggtt
     6781 tcttctcaaa tcttaaagaa aaaatcgtta acagcgatga cgtaacaagt ggtgttaacg
     6841 tagaagttgg taaaacacaa gttgcagttg acttaaacgt tattgttgag taccaaaaaa
     6901 atgttccagc tttatattca gaaatcagag aaatcgtatc ttcagaagtt gctaaaatga
     6961 ctgacttgga aattgttgaa atcaacgtaa acgttgtcga catcaaaact aaagaacagc
     7021 atgaagcaga ctcagtaagc cttcaagatc gcgtatctga cgttgctgaa tcaacaggag
     7081 aattcacttc agaacaattc gaaaaagcta aatctggtct tggatctggt ttctcaactg
     7141 ttcaagaaaa agttagcgaa ggtgtagaag ctgttaaagg tgcagcaaat ggtgtagtat
     7201 ctcacgaaaa cactcgtgta aactaagata aaataaatat aacaggagaa attatcatgt
     7261 cagtagaaga aaaattaaat caagctaaag gttctattaa agaaggtgtt gggaaagcca
     7321 tcggtgatga aaaaatggaa aaagaaggag cagctgaaaa agttgtttct aaagtaaaag
     7381 aagttgccga agacgctaaa gacgctgtag aaggtgctgt agaaggtgtt aaaaacatgt
     7441 tgagtggcga cgataaataa ggttaaaagt tactttatct ttttggtaac attagtcaaa
     7501 agagtctgat caagatgatt ctcagaaaac aaaaagctag agattcccaa ttgcggaact
     7561 ctagcttttt aattttgcct ctttctctta ttatatttca gcaggttgtt ggccatgagt
     7621 atgaatccca tgtcaattct cacttgacgc ttacctctca gatgacatct cttataaccc
     7681 aaacaaacct ttatctgccc aaagacagat ttcatatcaa tcttacgttt agcgaaaatt
     7741 tgtctaccct tggaagataa aagtgcctga tattctttag tttttaaaca ctggtaacgt
     7801 tcattcatat acagtccctt ttgaggggct gattcaggtt cataatcgca gtcaacattg
     7861 atttcaaggc tgtttgcttt ctatctcccc tttttcatga tatattttcg tacaggttct
     7921 tcaatagttt ctatagtaaa atgaaccaaa aatagtacac gttgcggtat aatgttcttg
     7981 tggcatattc attagatttt cgtaaacagt tctctcctat ttgtgagcga acaagtatag
     8041 tcaattgatt gtcagtttga agaccaagag tcgtaaggca aaggatatgg cagaatccat
     8101 ccaagggtgg ctggcacaat ttctagtgaa cctctttaaa tctattacgt ttgactgtgg
     8161 aaaagagttt tcaaaatgga aagatatttc caatcatcac gatagtgaga gcttttttgc
     8221 gaatctagga tgttcaagac agcggcgtct caacgaacat tcaaatagac ttttaaggtg
     8281 tcatgacttg ccaaagcaga cagatttcaa cgaagtcagt caagaatttt aggctggaca
     8341 aaaactcaat ttcaggagaa aatgaaataa atattcccac aataaaacgc atagtatcaa
     8401 agtttttcca acacctgata ttatgcattt tttaatttta aagacttttt cccagcctca
     8461 agaaatcaca tcccacctaa atcctttgga taccaaatac ctcatcaggt attttctgag
     8521 ttggtaagaa tgtctagctt aatttgacaa atggagggcg ttaaatccag tttttacagg
     8581 aagtctattt aaatattttc acactgacct tacaaaatat gaaataaatt atataaagtc
     8641 attcccatga caaagaataa catcccttta aaacagtatc ttacccaatt ttcaggtaag
     8701 acttttgata caaccgtccc caatacccct ccgcatatag aagcaagaaa aataactgga
     8761 acaggtgcta aatgatactg atttaaacct gtaaccacaa caattgtagc caaatgagac
     8821 agctgggaaa agaaaataat tgcaagagaa tacatcgttg cttcttttat cgaaatagaa
     8881 aaaaacacca tcaataaaga tacattgagc ggaccaccac caatccctaa aaaactagaa
     8941 attaagccca atagaagacc acaaatcata tagaatccta tcttatcaaa ttgataagtt
     9001 tttttaaaat ctgttaaaac aaatgataca agtagcatca caaatagtaa aatcatttga
     9061 acaatggata ctagccgtac tgataactga gataacaaaa cttgaaaaat aagatgacct
     9121 agaaatcctc ctaaaacaga agaaaaggat accgtaaaaa tcaatctcca ttttatctct
     9181 ttactttgtg aaaatctttt gattgttgaa ataatcgcca ttataaaaac agagaaacta
     9241 gaataaaaag caatatcact gaccgaatgg tatccaaaac tatccataag tggcttaatt
     9301 ataattccac cacccatgcc tgatatagaa cccaatccac tagctattat aatgattaga
     9361 aaataaataa tctcaaccac tttttaagtc catcctattc tttcaaacat ctaaaaccta
     9421 gatggctact agtggaagta gagatacatc catttctagc agctactcgg tatcgattac
     9481 aataagaaca atgacataaa aaggaacctc ctctgatagc atactcctca tcttgtatcc
     9541 catattttgg caactcatag tcatcttcat taaaactagc taataaggta tatctcggat
     9601 tgcgacacca ttcccaaaca ttcccaatca tttgatatag accaaaatca ttaggtaaaa
     9661 attcatagac aggagccgtt cctataaatc tatctaaggc agtattttca taaggaaatc
     9721 ttccttgcca agtattggca tgataatatc caccttctaa aagagtatct ccccaaggat
     9781 attcagattg tcttcctcct ctagcagcat attcccactg tgcttcagta ggtaaagaca
     9841 ttccgctcca attacagaaa gccaatgcat cttctaaagc aacatgaact actggatggt
     9901 cttctaaacc cactagatta ctgttctcac cgtagggatg tttccagcaa gcgttcggaa
     9961 cttgtaacca ccacggagcc ccagcaggat gaggatatcc ctccctttct gcctctggaa
    10021 cgaacaatat aaaaacaaaa gaccactctt gtcgctcagc tagtgtttta tagcctgttt
    10081 ctttcacaaa ttgagcaaat tcttgattcg taactggagt atccgcaatg gaaaaatcct
    10141 ttacactaac agcgacttga ggaccttcaa aatcatctaa aaagccctct tcagaatttg
    10201 ttcccatcat aaatgtccca cccttgattt ttttcatttt catgattgac tatcccccgt
    10261 tttcgttaga gttggcgtta ttttagaaag ttcaacagga actaactctt catccacaac
    10321 aaaaccttct tctctatatc taagaaagtc tactaatttt ttcttcatct gacgaacaat
    10381 aggttggtat ttttcgcttg gatacaaatc attcatttca tgcggatctt ttttcatatc
    10441 aaataactga taatgattta aaacaggaaa ccagataaat ttccactggt ctgtcaagat
    10501 atattgacta gaatcttttc ctaaagcatg ttcaccatga aattctgttc gccacccctc
    10561 gtactgtcca aataataaat tcttaacact ccgtccatct agttcatctg tagtagttcc
    10621 tcctgccaaa tccacgagtg aaggaaaaat atcttgaatt ttcactaatt gcttgattgt
    10681 tcccctatta ccagctatca atccagccgg atcgtaaata aacgaaggaa tgtggatact
    10741 tccctgataa ggatatccct ttctaaacag ataatgttca ccaagctgat ctccatgatc
    10801 cgaaacaaac cagataatcg tatctttatc atgacgaaat tcttttaggg aagtcaaaaa
    10861 gcgactaatt tgatggtcta tatgagtaat caatccaaaa taagcagcaa ccattcttct
    10921 ttgatcatct tcctttaatt tccctcttaa agcataaata gacggaattt gcttttctaa
    10981 tacttcccaa ttcccgatat gcaaatcgag aaattgaggc aagcgttcca tatacatatc
    11041 aaaatagtat tttgggggat ttaatggagc atgtggtttt tcaaaagaca ttttgagaaa
    11101 aaaaggaacc gttggatctc ttctttgtaa aaatgaaatg gactcactca caacccagtt
    11161 tgttggatgt aacttctcat ctttatccca aggtctagct tcccaagaat tacaatccat
    11221 tccgtcatca atcaaatcaa catcataacc aactttaccc tttaaaaagg ctagatagtc
    11281 actaacataa tcaaattgac ttccataagc cttatcatac tttctatcaa catgtagata
    11341 cccatcgtga agtaagacat gatcaaaccc aagtctctgt ctggaaggaa aaacatgcat
    11401 cttaccaata cactctgttt gataacccat atctttaaag acttttggaa gagtattagt
    11461 aaaattccaa ggcacctcat cttgataacc aacacgtcca cttttatctt ggtctaagcc
    11521 agtcaataaa gctgccctag caggaacgca agaaggaact ggagaatagg cattttcaaa
    11581 attataacct acactagcca tcatatctaa tgttggagtg ctaactagct tatccttact
    11641 attcaaggat aaggcgtctg ctctcatttg atctacaaca atcaaaataa tatttggctg
    11701 ttttgtctga accataaaat ctcctttcta atatggcaaa agaggcacaa gaagatatct
    11761 acctttactg cacccctttc tatatcaatc tctctatata aagcaataac attcttgtta
    11821 tgttttatag aacaatgaac taaaatatga ctaaatcgat taggaaattc aaatcatttt
    11881 ctagtactgt tttagaagtt acagtgtact attccaactt caacaaacta taaaccttgg
    11941 tctaataaca attttagtgg agataagaaa tcctacacct aactcatctt acacgtaatc
    12001 tatttctatt ttatcacaaa aaacgcaagt aagaccatta actcaattca gttttatctg
    12061 ccattttcac aaatgggaaa taagtcaaga cactaataat caaacaaaca actgataaga
    12121 tgatggcacg ccaatcaaat gctgtagaga agaaaccata taaaattgga ggcattaccc
    12181 aagtaacatt ttgtgtaaca ggtgaaacaa gaccccagct tgttgcccag taagctaccg
    12241 ttgccatgaa aaccgggcta agtacaaatg gtataaatag caaaggattc aagacaactg
    12301 gtaaaccata attgaccggc tcaccaatat taaacagaac tggtgctaga ccaagtttag
    12361 caacttttcg ataatgactg tttcttgaaa aaattaaaat agcaagtacc aatcctaatc
    12421 ctccaaacca gacaaacgcc ccaaaagacc cacttgtcca tatataagga atcggttcac
    12481 ctttttggaa agcatccaga ttcgctaaca tagcaactcc aaacagccct tccatgatgg
    12541 gagccaatac atttcctcca tggagaccaa aaaaccagaa taacttattt aaaaagatca
    12601 tcagaataac tgcaaagaaa ctttgagaca aacctagtaa tggcgtttgt aacaccttgt
    12661 aaacccaatc aatcaataag tcattgctaa gtaaatggaa aacataagtc aagatggcta
    12721 ctatatacat cgccataaat cctggaatga tagaagtgaa cggcttagca atcgcagggg
    12781 gaactgaatc tggtaacttg attacccagt tctttttcat tactttacag aaaataatag
    12841 aggctaaaaa tccaatcatc atggctgtaa agtagcctct ggcattaata tggtttcctg
    12901 gaatcacatt cccaatagtt accatcagat ttttaccatc aaatgctaga ttatcaattc
    12961 cttgtaagat ctgatctaat ttcacatctc ctacatttgc caaagggaaa ctctttgtaa
    13021 ctgtacttcc aatcgaaatg acaaacgaag caagtgatac caaaccagca gaaactgtat
    13081 caaccttgta aatcttagcg atattcactc ccaagcaata gatgaacaac aaagaaacaa
    13141 ttggtatact tcccttgaat accaaattat tgatgtcaac aagccactga aaggttttcg
    13201 taatactttc taggtgaaat tgttgtggta aatccactag aaaagcattt aataacaaag
    13261 caatggaacc tgtcataata acaggcatag tccccacaaa tgaatcacgt aaagcaacta
    13321 aaaacttctg attcccaact ttcattgcaa ttggagaaag tttttcttgc attttatcca
    13381 acattgtatt catactaaac tcctaatttt tttcaaatcg ttcgtatact tctaccactt
    13441 ctttcgccaa gtcaacaaaa gtcagactcg tcattaaatg atcttgcgcg tgtaccatca
    13501 agaggctaat tgatgtttct tccccctgtg cctctcttgt caacatttct gtttgagcct
    13561 tatgcgcctg taataaggca gcattcgcat cagctaatct tcgattggct ttagagaaat
    13621 cccctttttt tgctgcctga atagcctcca tagcattact tttggcttcc ccaccataca
    13681 taattagccc cattacagat tctaaattac tttcatccat aacattctcc tcaatcttag
    13741 tctaataaac tttcagctaa atctaaaact ttagaaccat tcatcattcc gtaatctgtc
    13801 atcggaataa ccgccacagg aatctgtcta tcttttagtt tttcttgaaa gtcccctagt
    13861 aaatagcgaa cttgggggcc taaaagcaac acatttactt cttttgttgc tacaatttct
    13921 tctgcttcag gagctggaac tgcaaaaatt tctgcatcca accccttatc ttctgctgcc
    13981 ttttgcatct ttgtcactag cagacttgta ctcatacctg ctgcacacgc taacataatt
    14041 gttactttag ccatagctta ctccttttat tttattgtca atgttactgt ttttcgatca
    14101 actttagact cataatctaa atgtttggga cgactagtat aatgcaaatt ataaactcct
    14161 ggaggcattt gtgaaatctt ttctttttca gttttattgc gaattaataa atctaccgaa
    14221 gtattccctc caaagttatt aaacataggt tcattttcaa tagaataatc taactcatat
    14281 ccactattag ttgtaattcc ttttaataaa tcaggaacat ctcctctgtt tattactata
    14341 tccttagcgc catttatttt gggagattga ctataatact cttcagcttg atgttgttgt
    14401 aaatcatttc tagagtaaga aacataatca actacaaatt cagtccctga taaattattg
    14461 tccactaccc caggccaact tcctcccatc gctaaattta actgaacata ctgaggtcta
    14521 ttaaaaatct ttttcgctat attatctgat ccataatata cttttctaac aatatgattg
    14581 tcaaccatcc atacaatata atctttatac caatcaattg aaaaagtatg atacgaatca
    14641 ttaaagtttc cactcgataa tttaaaggct gtaccacgtc ctgcaaattt tccatcatca
    14701 ttatccgact gtccataatg aagcgtttga taaactgtct tattcccact tccatttgaa
    14761 tttcgttcac caactaattc cattatatct atctcacctg ttgacggcca tcctcgtccc
    14821 tgaacaggat taatcttccc atcaagtgta aaatccgatc caagagtcca gaaagcagga
    14881 aaaactcctt gacctttcgg taattttgca cgcatctcta atttaccata aagaaattct
    14941 actttcccat gtgttctaac acttcctgaa ttatatacaa cctttctgtg attgtttctt
    15001 gggttaaaat actgatcttc cttagagcga ttcgtagctc taagaactaa atttcccgaa
    15061 cgtagaaaaa cattctcatc acttcttaca taatgttgct gttcccaacc acgaattgat
    15121 cctaattcat atccccaata ttttttattt aattgttcgc catcaaaatc atctctccaa
    15181 attttcttat atgaatttcc tttagacagt tgatacatct ctacattatc aataaaaata
    15241 ttagattttt ttgtagtatc gtcttctgac catttcacta attgaatcaa aactttatcg
    15301 tgtttaccgg aattgaaaga aaacatgtat tttttccatt cggttgtatt aactgtttga
    15361 tcaacagctt ctccaatgtt attttgaaca atctctcctt gcttattatt atcaaaccat
    15421 cttgcggtaa aatatccctc tgctccacta atatctactt tggcaaaagc agatactaaa
    15481 taatcttcat taggttctat atcaataact tgtccaatat atccatctgc tctattatca
    15541 ttaattgcag tagaaggaat catacctgag taatgtccgt tactataact ctcatttgaa
    15601 ttaattattc ccctatttct agagttccaa aacagctctc cttgctcaaa atttgaattt
    15661 tttattaaat tttcattagc aaaaactttc acatgaataa atattaataa aaataatata
    15721 aataaacttg aaattaattt cttattcttc attttatcac ctatttactg acctagccaa
    15781 gcgagatact gtatgtctca ctcctctaat tgccttgctc aatgcataaa tattatcgac
    15841 tgttgccagt cccccatatc ctgcgtcacc tatatgttga atatcaactc cacaaatttt
    15901 atttctaagt gcaatttcct tgatagtatc tgtatcagat gtttcttggc tagtaccaat
    15961 agcactcagt actagcccac ccttactatg aacgagatca acgacttcac gcaactcttg
    16021 gtcatgaaag gctggaacgg ttccaactgc tggaacaagt atcacatccg cacctgcttc
    16081 caacaattgc tctgcgacag aaagctctgc cacaggctca ttcactcctg ccccgtgcat
    16141 cttacctgca ataatcaaac cagaaaagtt ttccttagca gtttgaacag ccttaatgat
    16201 ttctcgattg ctaactcctg ttccaggatt tccagtcaaa cagacaaagt caaaacctaa
    16261 ctcctctatt cgattcaatg tttcaacact agcaacacga cctgcaacaa tttcctgtgt
    16321 ttcctctagc atctttgcag atgggtcaat cggttccaaa tttacaccaa ttggacaagc
    16381 aacaagctgg tgtaagcgat gaataacatc atcacctgaa ctatccaaag cataaatttt
    16441 aggctcaaaa acatcaacac aattcaataa aatcatatca gctccaaaag ctctagcaat
    16501 ttcagaatta gttatatccc caacaaatgt ctcacgagta actacatttt cagatagaac
    16561 aactctacct tcacttgcta aaatactttg ttttaattct tcagcattca tctgtaatat
    16621 ctcagatgga tttgcactaa ttaaacgttt cattctcaat cctccaataa cttaaatcgt
    16681 ttttcaatag ctattacaaa tggtaaataa agtaagatat ccaaaacaat caatacaatt
    16741 tgtagtaatg aaccagaaat acttcctgta gcaagtaaac cacttataac aggtggcata
    16801 gtccaacctg gatctgtata agttagcgga accaaacctg atgccattgc tccccacgct
    16861 actagtaagt taaacattgg agctaagata aatggaacta gtaacaaaat gttcaaaaca
    16921 ataggaacgc caaacattgc tggttcattt atattaaata atccaggtat aacagttata
    16981 ggtgccaaag tttttagttg tttactagct ttcttcttat gtgctaaata tcctaacgca
    17041 agaactaaac ctatggttgc accaccgcca cctatgaata caaaattatc cataaatacg
    17101 ctagtaaaaa tatttggaag agtttctcca gtttgataag ctactttatt tgcatctaga
    17161 ttagctaacc ataacggttt cataactgca ttaacaacat ttcctccgtg taatcctaca
    17221 aaccaaaaac tactattaag gaaaattaag ataagaagac caataacatt atttcctaaa
    17281 agtccaagtg gacctcccaa agctacttga gcaatttcat gaagattagg taaacttaat
    17341 ttatctaaag ttgcataaac aatcaaccaa ccaacaatag tcactgcacc aggaataatg
    17401 gcactaaagc ttttagatac tgctggtgga acaccatccg gcatttttat ctgtatatta
    17461 tgatttataa accattggta gatatagcca ttaatcaatc ctaaaatcat agcaacaaat
    17521 aaaccttttg atgccatgaa agcggtaggc attcctgctc cagcctctcc tctaatgaaa
    17581 ggcgtaactg taataaaaga tgataatgac acaataccag caggaatacc atcaactttg
    17641 tattgtctag ccataaagta agcgatacca aaactagcta caagaccaag taatccaaaa
    17701 ctactatcaa cacccttcca taaatagtca gcaacaccaa tatcacccag atatttttcc
    17761 catcctggaa taggaaaact tgcaataacc attagtaatg aacctatcaa caataaaggt
    17821 atagttagag taataccgtc tcgaatagaa accaacgctt tattattacc caatctagcc
    17881 gctactggaa gtaacttttc ttgtaataat ttttccataa ccttttcctc ttaaatatat
    17941 tttattatca aaagttatca attatcttta ttttttgacg tcaagttgtt atttgattat
    18001 cttttgttat ttatatttta caactataag aaagccttgt caaggatttg tgtaaacatt
    18061 tgattatttt ttattatcaa tatataataa aaagagacca gtaagtaatg aaaagcttaa
    18121 aaagattaac atctacattt atccatagca aaaaatcata ggattttaag aatctgacaa
    18181 ctaggaagaa aactaatatg accttatcta agaaacaatt acaactaaga gctaaaatac
    18241 tggaaacagt ttacacatta ggtcccattt cacgaattga aatcgctact aaaacaggaa
    18301 taacacctgc aacaactagc agtatcacaa atgatctgat taaagaaaat attctcttag
    18361 aacttggtga agacgaacac gatacaagcg tagggagaaa aaaaatcctg ctagacattc
    18421 aagctaaacg attttactac attggttgtg aactatctga aaaacatttc acttttgctt
    18481 taggagataa tctaggaaat atcttaaagg aagagaaaga aattgttact aaacagttaa
    18541 tccaagaaaa gggaaatcaa ctcattaacc agacactaaa gcaattctta aataactgct
    18601 ctgattatga aatagaagca attggaatcg cgctacctgg tcgctattta gatgattata
    18661 aaataacaac aaacaatcct ttatggcaac atatcgactt agaaatgatt caatcccact
    18721 ttgataagcc tttgtttttc tctaacaatg ttaactgtat ggctataggt aaacgcctat
    18781 ttagtcgcca acagaatgac cctaattttg cttattttca ttttgctaga ggtatgcact
    18841 gttcttatat ctacgacgga aacatttatg ggaaaggaaa tctaatgatt ggagaaatag
    18901 ggcatacagt tgtttcatct gagggagaag aatgtagctg cgggcgcaag ggatgcttac
    18961 aaacttttgc tggagaatct tggttaatca aaaaatcaaa aattctatat catcaatctc
    19021 cctattctct acttcctagt ttagtcaaaa atgccgatga tattgatatc caagttattt
    19081 taaccgctta tcaattaggt gacactggaa taatcacact tatccatcaa gccttattct
    19141 atcttagtca aaccatttta aatatcagta tgatgattga ctctcaaaaa atctacctac
    19201 atagtccttt actaacaaat caacacatca tccaaaaact atatagtgaa atgaactaca
    19261 aacccaaact gctctataat cgcctacctg aagtcattat tgaaccttac aatgatttta
    19321 cagccgcaca ctcagctatt gcactttgtt tgtatcatac aattctacat tcttaatttt
    19381 ctattacatt taataaatag gctaaaaaga gggagtatat cataaaaagc gaataaacct
    19441 gttttctgaa aatcagaatt ctgttttgtt cgctttcttt atatttggaa ttatactcct
    19501 atctcccctt tttcataatg tatagtaaaa tgaaataata acaggacgaa tcgatc
//
LOCUS       DAVBCN010000036        19196 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVBCN010000036 DAVBCN010000000
VERSION     DAVBCN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19196
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.036"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(<1..1879)
                     /locus_tag="VQ497_001174"
     CDS             complement(<1..1879)
                     /locus_tag="VQ497_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET2517485.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(1873..2742)
                     /locus_tag="VQ497_001175"
     CDS             complement(1873..2742)
                     /locus_tag="VQ497_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2517486.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPDLENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNILCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQDVKKAVDNFVDTYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(2826..5471)
                     /locus_tag="VQ497_001176"
     CDS             complement(2826..5471)
                     /locus_tag="VQ497_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HET2517487.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDERFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELIVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAFRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVFRYYNMSQENVHISEHQQTILDLLE
                     RPYPVHSGPLDPQEIRTEFIKKEEI"
     gene            complement(5562..6842)
                     /locus_tag="VQ497_001177"
     CDS             complement(5562..6842)
                     /locus_tag="VQ497_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HET2517488.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSIDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            7013..9097
                     /locus_tag="VQ497_001178"
     CDS             7013..9097
                     /locus_tag="VQ497_001178"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HET2517489.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKTSAQADWENLLHRFDIIEIGEADRTFFDHCLYRLFLFPQTF
                     YEINESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAT
                     SQNYRQLFDTETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKVCQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHSHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPNH"
     gene            complement(9137..10816)
                     /locus_tag="VQ497_001179"
     CDS             complement(9137..10816)
                     /locus_tag="VQ497_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HET2517490.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGACLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSMPLLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            11572..12801
                     /gene="arcA"
                     /locus_tag="VQ497_001180"
     CDS             11572..12801
                     /gene="arcA"
                     /locus_tag="VQ497_001180"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HET2517491.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            12859..13875
                     /gene="argF"
                     /locus_tag="VQ497_001181"
     CDS             12859..13875
                     /gene="argF"
                     /locus_tag="VQ497_001181"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET2517492.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            14040..14987
                     /gene="arcC"
                     /locus_tag="VQ497_001182"
     CDS             14040..14987
                     /gene="arcC"
                     /locus_tag="VQ497_001182"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HET2517493.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            15196..16707
                     /locus_tag="VQ497_001183"
     CDS             15196..16707
                     /locus_tag="VQ497_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HET2517494.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            16729..18060
                     /locus_tag="VQ497_001184"
     CDS             16729..18060
                     /locus_tag="VQ497_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET2517495.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQNVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKCVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNDLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            complement(18393..18683)
                     /locus_tag="VQ497_001185"
     CDS             complement(18393..18683)
                     /locus_tag="VQ497_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517496.1"
                     /translation="MGVLGYYEAFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
     gene            complement(18671..18820)
                     /locus_tag="VQ497_001186"
     CDS             complement(18671..18820)
                     /locus_tag="VQ497_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HET2517497.1"
                     /translation="MGDQPVQDTETSSALILSHYLDEQDLSEKLKSDLQWFELENKLL
                     NLWEY"
BASE COUNT         5400 a         4323 c         3918 g         5555 t
ORIGIN      
        1 aagtcgtata aatcgtcgaa gctgtcgcaa tggtatgcca ctggttagct gtagttgctg
       61 cgaaatcctt gtctgcgtag aagtcagtaa atggtaggat ttcaacttcc agctcgtcga
      121 tgcggtcaag ctggccagcc agataaacct cgatacggct ttctgctcgt ttgatgcgtt
      181 gcaagagtcc gcccatacgg atgtcaactg tatccaaacc aaagaccttg ttttctttca
      241 gccattggtg actaaagagg gcatggaagt cttcaatttg gcttctaagt tctggtaatt
      301 cttgtctggc gatttgttgt aaactttctt tatcatccgc ttggtaggcc tgacgaatgc
      361 gtcgtcccac atctactttg ctacttaaaa tagcattcaa ctgggcctga gtttcaaaga
      421 gataagcata gtttccagct ttttctttaa tgttagcaag cgtctcagca gcctgagcga
      481 agtgcggttt gtcctgttca ggtgtcatgt gttgatcaag aatcggacaa agaatatcct
      541 gataaaaaac atagcggttg ggattgatac cgctgagatt gcctggtagg tctggtaaga
      601 ggttggcaag gtcaatctgc ataaaatcct caaccgttag accagtattg gtcttgaagt
      661 gcgcagacaa accatctagg tcattgcgat agctgagttc tgcccagatt tgcaagcttg
      721 gtaggataga gaactgggca gtttcaccac cattgtctcc ccaacccgtt acgatgactt
      781 ctttaatctg attggcacgg caggctttat tagcctcgat agccactaga cggctaaaat
      841 ggttgtgagg tgtaaagcca atccacttcc aagctccccc tgcaaatgca aggtcatggc
      901 taatcttgtg atgattgcgg aaattacggt tgtatttttc ctcgctatcc tgataataat
      961 cccagtaaac cagagtcaca cggtctttga gacggtctag gtagacacga gtttcctctg
     1021 gaatttccac atcacggtcg tactggccat ccgctgacat gagtttgaag aacatatcac
     1081 tccacatctg gcagtggaaa ccatatttgt cagcaatatc cagcacgcgc tccaagtgtt
     1141 ggcacatgag gagactacga tccacaacac cgttcaaaat caggtagcgt cccaaaccaa
     1201 ccaagtgggc ttcgtccatc ccgatattga ccttgcgagt cttcagttta gacaacgtgg
     1261 caaacatgcc atcaatcagg tcataaacct tttcttcgcc aatgagaaga atgtcctcta
     1321 catcacggag ctcctgcact tccttgacac cccatttgac aaaggccgac aagtgggcca
     1381 aggtctggat gcatggtaca aaggtcatgt caaactgttg agcataggct tcgatttcct
     1441 gcaactcctc tgctgaataa gctccacgga aatagccaaa gtaaggctgc ccttcaatct
     1501 ggtaagtgtc ttccatgtaa agttcaaagg ttgagtagcc catgagagcc aagacctcaa
     1561 tcatctgctt ggcagaagcc acattcagca ccgcatttcg agaacagtca accatgtaag
     1621 ccaaatcttc gtaagctgct tgttcctcaa tctctacttt atcagcttct gctagaactg
     1681 ttaccaacaa ggataaggca cgataaagtt ggtgaggttt gcggtaggtc aattgatagt
     1741 gaccttcctc accctggata gagatagagg cttggtcaga ctgagtgaca gccacttcca
     1801 catctggtag agaaatatga ccttttaaaa cctcaatagc ttgcgtttgt ttgggactaa
     1861 gtcctgtaaa tcttaccatt gcttttcctc ctgtagccag ttgacaagag caccgtagag
     1921 attggcatct gcgtgatagg tgcaggcctg gataactggt gcgaccgtgt attcttcgta
     1981 ggtatcgaca aagttatcaa cagccttctt gacatcttgg ataaaatctg gattttgact
     2041 gatagagcca cccagactga tgacatctgg atcgatcaga tactggatat tgagcaagcc
     2101 ttgcgccaga ttgcggttca tgcgctcaat ggcttcttga caaaggatat taccagctgc
     2161 ggcctcttgg taaatcttgc gaccgtccca atcagtatga ccagattttt caatcacgta
     2221 tcgtaccata ttcccagttg atgctagttg cgaccagtta ttaagttttt cagcaggggc
     2281 aagggttgtc atgtagccaa attctccacc caagccgtgg cgacctcgat gaagtctacc
     2341 attgataatc atggctccgc caatccctgt cccaatcacg acacaggctg cattttcaag
     2401 atctggatga gctagtagtt cactgagtcc aacgcagttg gcatcatttt ctaaatggac
     2461 aggtagctga taagagctaa gcgcctcata ccaagaaaag ccatggatgt agggcaccgc
     2521 actgaagcca tcaatcacac ctgtctcttg attgactgca cctggaacgc tcatagcaat
     2581 cccactgtaa tcctgttctg acaagcgttg atctagccac gctagtaaat cctccaagtt
     2641 ttcaggcgtt gaaatacttg tcttatccag tattttccca tcaggagtca gactggcaaa
     2701 cttaatccca gtccctccga tatcaatcgt tgcaatggtc attgttttta ccataaaaag
     2761 gggcgaatca gcagttagtt cttacttttt cgcccctcct ttcttttgat gtttactttt
     2821 tgaaattaaa tttcttcttt tttgatgaat tctgtacgaa tctcttgtgg atccaatggt
     2881 cctgaatgaa ctggatatgg tcgctcaagt aagtcaagaa tggtttgttg atgttctgat
     2941 atgtgcacat tttcttgact catattatag taacggaaga catagccttc ttcattttcc
     3001 gccactttaa aggctgttgg acaaacttgc ggtatgctga gaacagaatg gctcaagagg
     3061 ctaccagtcg caaccacgct tccttcctgt ctagcaagct gaaggctggt aaacggtgtc
     3121 tgcaaggctt tggcacgacg gaaagctgaa aagcgttctt gggcttggtg gcattcaagt
     3181 gcaaactcga cttcaaactc acgcaagcat tgtgcttctg gcgttgggaa gtagccccag
     3241 tcacctagct cacctgatgc acgcaaaatg gtcacggcaa tggtgttatc cccaaggatt
     3301 tcgtattcat tcaatccctt attggataca gtcacccctt tttcatcgtc atacagactg
     3361 acaaaagctt gttggtgttg aggattttca ggattttccc atgaagcagc tggtttgttt
     3421 ggtcgtgtca ccacctcata gatactttca gaatcattgc ttggacgcgt gttatgagtc
     3481 ttgaccaaga gacggatacg gtgatccttg gcagtgttag taaagcgagt cttgaagcgg
     3541 atttgtggat tgtcaacaaa gacaatcaac tcagtttcca gaggaatgtt tgtcaattct
     3601 tctgaccgtc cagcctcacg cttcataaac tcgatgatac cttgttgctc ttcttctagc
     3661 ttttcatccg cactgacagg cacggtcaat tcatgtttga gcaagatttt agcatagcga
     3721 tctgtgtttt ccaagacctc gtggccctta agctctgcaa agattggctc tgttcctttt
     3781 ggttggaaat agatatactc gtttccgatg tccccacggt cttcgaagcg gataaagtct
     3841 tcataggctt cgtgagttgt cttgtcatat actgtgatat tgtcatccac actcaccgtt
     3901 acgaatggtg tatcaatcac tccgttttgg taaataccct cacggtgttc ttgttttcct
     3961 tccagcaatt ggaaggttgt ccaagaaagc ggcgctaggt gaactggaat ggtcacgcgc
     4021 acttgtcgag caatacgagc ttggcggaac ttgtcttttg gtaaatcgta ctcaaaatta
     4081 gctccgaggt cttcgattgt agcctctaca ggacgaccat ccaagtcctc cacacggtaa
     4141 cttggtaaga taagagcagc catctttttg tagccttctg ttgggtgcaa ttccttgaaa
     4201 tcacaagtcg ccacatcaat cactgtgctg acagtatcga ccttatcatg caagcctgtg
     4261 ttaatgacag taaagagata gtcactttga gccttatccg tagcaatttt acccttccac
     4321 tcgttgagca agttactttt aacaaagttt cctacttggt tgaccttggc aaaacgcgtt
     4381 tccatctcgc ggtgaacttc gtccacgcta cagccacaga tactatcatg tggcgcattc
     4441 tgcaaaagtg ttttccaagc ataggtcaac tggtccttgt ggttgtgtcc accagtgata
     4501 atagtcaagg gttctaccac ttgctctagg aggttgctat tttcttggaa ggcttgtttt
     4561 aggtaaatgc gggatgaaga agtgttggca agtgtgtacc agccatctgt ttcctgactg
     4621 gtcaactcgc ctgtaacagt tgataagtgt tcaggaagcg caccttctac agcttgaaca
     4681 tattcatcaa aagaactatg aacaaagatt acatccggga agagttcatt tgccacacga
     4741 atggcttcgc tcagattttt ctgtacaggc tggtggtcac agccgttcat catcaaccat
     4801 tggttggtcg aagcgtaggc acgcacatct gacaattttt gtttccagaa ggtcaaggcc
     4861 tcatctttgt caactgggat ttcattccca ttactgtacc agttggcaaa gagaatacct
     4921 aaaacacgac taccatccac accctgccag tacatttctg aaaactggga tgtaaaccgc
     4981 tcatcttcaa ggacttggtt gtcaaatcca atcggcttca caccacgacc aaaggctgcc
     5041 acgtgaatac ctgatttttg aaggatttga ggagcttgtc ccatatttcc aaaggtatct
     5101 ggaaagtagc caatctgggt tgatttaccc catttggcag cttcttgttg accaatcaag
     5161 gtattgcgga cattggcttc actggagatc aagtagtcat cctgcaagat gtaaaaggga
     5221 ccaattttaa gtttgccctc gtcaatgtag cgttggacct tgtcgcgatt ttcagggcga
     5281 atttgtaagt agtcatcaag gacaatagtt tgtccatcca agtggaaact cttgaactca
     5341 gggtcatttt caaagagatc aaagagattg tcaaacaatt ccaccaactg catacgatgg
     5401 ctttcaaaag gcaagtacca ctcacgatcc cagtgactat gtgagataat atgtacaaca
     5461 acattttcca tgaagtaaaa cctcattcta aatttaaatt ttaacgtttt aaatgtaaac
     5521 ttgtgattct gataagaatc ggttgagcta aagcgagccc cttagcggat atccaagtaa
     5581 tccaagacca actcacagaa catcatgtta gcccaggaga accattcacg agagtagagg
     5641 gtcggatcat ctacgtggaa gctttcgtgc atgacacctg taccaccatc gcaggcaacc
     5701 agctgatcca gcaagaattt tttctctgcc ttatctcttg ttgtcaatcc ttggatagaa
     5761 agggcgattg gccagatata gcgatagaag gtatgagaac ttccgagacc gctagcgtat
     5821 tctccttggt agaagtatgg attttcagag ctcagaatgg tacggcgagt ggcttgatac
     5881 acttcatcat cgatcgaaca gtagcccaga tagggcgcag ctagtagact tggtacattt
     5941 ggatcatcca tgatgctggc atttcctagg ccatctactt caaaagcgta aatcttttcg
     6001 cccttgctgt tggtggtgta agcgtagttt tcgattcctt cttggatttc atcctgaaga
     6061 cgcttggcat cagcaataac actctggcta tcagctaggt ttaatgctgc gaagatttct
     6121 tgcacataac ccaagactac tacagcaaac atatttgacg gaatcaagta actatactgg
     6181 caacagtcat cactcggacg aaaagctgac caagtcatac ctgtcactgc aaagtcaggt
     6241 ccaaagccat catttaccaa ggtgtcttcc ttacggtccg tatctcggac aaaacgataa
     6301 ggagagttct tgtggtcttg ttcaactgtc catagatgga gaatttcctt agtcgctgcg
     6361 acaaaagttt catcaaactg actagtctcg ccagtctctt tccagaggag ataagccaac
     6421 tgcaaaggat agcaaagcga atccacctca tacttgcgct cccagatcca gccgttaagg
     6481 tctgtgtggt cagtctcgtg gtgccctttc cagttctcct caatgttgaa ggagttagca
     6541 tagggatcct tgagtaccaa ggtcatctga cgtttgacca aacctgcaat ggtctgacgc
     6601 aggagggcat ctcttttagc tacatgaagg tagggtctga gttgggctgt cgaatctcga
     6661 agccacatgg caggaatatc cccagtcaag acaaaagttg aaccatcttc taagatttca
     6721 accgtattgt ccaaggtatc ggtgtagcaa cgctcaaaga catcaaccca ctctggatgg
     6781 tccttggccc gctctgctac ttcatctagc cattctctaa caatttcttt cgaataaatc
     6841 atcgtgcaat tccctctttc aaacaagttt catgttcagt atatagcttt tgagacagaa
     6901 aatacataca caaatgatag tcatttttct acaaaatagt tatctttaaa actccttttc
     6961 taaaggccct ctttttctga tataatgtag aaaaacagct aaaggaaaga ctatgaaacc
     7021 actacttgaa accatcgata cccgctttgg tactgctagc aagcatgcct tctctcgggg
     7081 aaataccctg ccatacacag gcgtgccttt tgggatgaat tactttgtgc cccagaccag
     7141 tgaccaggat ggatcttggt tttttgatcc gcatctgcct atctttcagg ggattcgatt
     7201 aactcaccag cccagtcctt ggattgggga ctactcttgg ctccttctga cacctgtcac
     7261 cagtcaactg gggggagaca gcctctttca ccgccagtct tcttatgata tagataaagc
     7321 ttgcttccag cctcactatt tgaagctctt ttctctgcgt tatcagattg aaacccagct
     7381 cacaccgact tgctatggtg cttctattcg tttgaatcaa aagcaaggca aagccctctc
     7441 cctctatctt cacgcagcag atgaactgac agtagagcaa gtagataagc ggactcttgc
     7501 cctgcgacaa gaaggtaaaa ctgaaactaa caaaaattcg ctaacgatgt tcactgccct
     7561 gcaaatgaac acggatattc ttgctatcag ccaagaagct ggggactggc gaattgactt
     7621 agcaagtagt caaaccgaga tgcaactagc cacttctttc atctctcctt ctcaagctct
     7681 aatcaatcta cctcaagaag attttgatag ctgtaaaaca agtgcccaag cggattggga
     7741 aaatctcctc catcgttttg acattataga gataggagag gctgaccgaa ccttctttga
     7801 ccactgcctc tacagactct tcctcttccc acagactttt tatgagatta atgaatcagg
     7861 gcaagccatc cacatggatc tggctactgg tactgtcaag cctggtgtcc tctttagcaa
     7921 caatggtttc tgggatacct tccgcaccac cttccccctc tttgccctta tcataccgga
     7981 gcactatcaa cgctttttag aaggtttcct caatagctac cgcgatactg gtttccttcc
     8041 aaaatggctg gctccagatg aacggggtat gatgccaggt acacttttag acggtattat
     8101 cgcagatagc gcctgcaagg acatggcccc tgacctagaa ggagaactct tccaagccat
     8161 gctcgaaaca gccagcaaag ccgaccctct cggcatcaat ggccgccacg gactagccca
     8221 ataccaagaa ctaggttacc tctctaccga ccaccacgaa agtgttagcc atactctaga
     8281 ctatgcctat agtgactttt gtatcgccag ctgtgccaaa aaactagaga acatagaaat
     8341 cgctgaaacc tacaaggcta cttcacaaaa ttaccgccag ctatttgaca ctgagacagg
     8401 ttacatgcga gcacgagata atcaaggaaa ctttcaccct gacttctctc cttatagttg
     8461 ggggcgagac tacgctgaat gctctgccat tcaagctact ttaggcgttc tccacgacat
     8521 ccctggctta atccaactga tgggtggaaa agaaaccttt agcaactatc ttttgaaagt
     8581 ctgtcaagat gctcccctct ttgaaacaac aggctatggt tacgaaattc acgaaatgag
     8641 cgagatggct actgctcctt ttggacaaat agccatttcc aaccagccta gtttccacat
     8701 tccttatctc ttccgctaca gcgattaccc tgactacact gcccttctta tcaagacgct
     8761 ccgtcagaaa gcttttcacc caagttggga agcctatcct ggagatgaag acaatggtag
     8821 tctctctgct tggtacatct ggtcagctct cggattttat ccaacctgtc caggaaaacc
     8881 aagctatgac ctcggaatcc ctctctttga ccatctacga gtctacctag ctaaagaaga
     8941 taaatggctg gatatccata ctaaacaaaa ccacagccat tttaactttg tcaaagaatg
     9001 ccgactggac aaaacactcg tatcaactat tcaacaccaa gacctcctaa aagctgaaca
     9061 actaactttc accctcagct ggctaccaaa tcactaataa ccaaaagaac ctttgcatcg
     9121 cgcaaaggtt cttcttttat gaaacttgga cttgaagttg atccacaagc tgaccatcca
     9181 ccgttaggtt cagatagaaa tccaaggttt tatctcctgc gaaatacagg gttggtagat
     9241 gcaaatcttt tttgctttcc cctgcttgga attgaaggac ttgaatctca tcctgatagg
     9301 cgacaccatg gacacctgtc cccggttgaa tcgaaatctt agcctctaaa gaggagctac
     9361 tttcactacg ttcaatccta aaatgaagag tctctccctt tgccacagct aggctttttt
     9421 ctgcaaatgc tagttcctga acaacttctt tttttgataa cccaggagtt ttataaaggg
     9481 aaatcttggt cagtagaggc atagattgtg cttcggtaat catcacgcgc accttctgtg
     9541 cctctactag cggacctcgc aagaggcgct tatgaccaac tgtgaagccc ctgccaaatt
     9601 cctgccagac accatccacc tctacttgca catggaaagc agcgattcgt tgccctagct
     9661 tcaaatcttc tcttaactca attacatcaa aagttttagg agaacctaag tcgagttcta
     9721 actggatggg caagtctgca tcgcttgccc aagagctggt ctcaaggccg tctgtcaaat
     9781 ggcgacaagc aaagtctgcg gaaagagctg gaccagatac ctcagctccc agagccaaat
     9841 cttctttata gagctcattg cgataggtcg caaattcata aagtcgttca atatcctttg
     9901 catcaaagag cccagcttga ttcggcggaa tattaagcaa gagtggagtt cctcgcccta
     9961 ctgagtgaaa gtagatttcg accaactcct cgagagactt aggatcctga tcctcatggt
    10021 agaaccagcc tggacggatg gaaacatctg cctctccgat tgaaaaaatc gtgcccgagg
    10081 gatccccgtg ctgaagatag ttcagctctg cttctgttcc tagtttatca ggattcacct
    10141 tttgccacag tggatcacct gcataccctc gttcattgcc aatccagcgg atactggtgc
    10201 cttctgttga aaaaatcaag caagcgccct gcaggtcacg aatggtttca aaccattttt
    10261 caaattcata attaaccttt tgcgcgccct ctcctctggc accatccatc caaacctcag
    10321 cgaacttacc agcattccca tagttaggat ttgataagat ttccttcaac tgagccagat
    10381 aataggcatt gtagtccgct tctcggtcca catgatagag gggactatgg gcatcccacg
    10441 gtgacaggta gacccccata tccatatcaa actctgtggc agcttgggat acttcaagga
    10501 gcaagtcgcc ctttcctctc ctccaaggac tgaccttaac cgaataatct gtgtgagctg
    10561 tcggataaag gacaaagcca tcgtggtgct tgaccaccaa aatcaacttt ttgaagcccg
    10621 tttccttgag cacacgaacc cactcacgcg catccaactg actcgggtta aagcgctcag
    10681 gatcctcctg tccagtcccc cattcttggt cataaaaggt attaggaccg aagtggataa
    10741 aagctgctag ttcatctccc agataagcta gctgagtctg acttggtaac ggtccatgcg
    10801 gtttgatttt cttcatcgtt atatatctcc ttctttagca acttctcttg ttcacaaggt
    10861 tatccacagg ttgtggataa taaaccatga tctaacagcc agctctttcc aactccaact
    10921 ggtaggctca ggatttttct cctcaatatt ccctcttcaa acagctctgt catatggttc
    10981 tgctaacacc tctcagaatg ctactctcca taattattct caggcttctt tcaggtcatt
    11041 gtgtgaagac cattcactct ccagactttt ccacagattg tgaataagtg attattctaa
    11101 actatcacct cgtcgactat aacaaaaaat aaaaagaggg tcaataagat aattatctct
    11161 tcctaaatac tcagaaaaga ttctataatt tgtacaattg aacaagaaat gcttctccaa
    11221 aatctatctt caaaagaatg ttgcatcttt ttatcctttc acacaaacat ttctctttat
    11281 acagatccaa gtgataacca gatgataatt ggacactttt tggttatttg tgtatttttt
    11341 gaaaaatttt ttaaaaaaat tttatatcct ctttaataga agtttatgca tttgtatata
    11401 tgtattccat cctatgttgt gtataataac ataaaattgt gctaaatatc ataatttata
    11461 ttattttccg agaaattata gccattgaca ttacctagga attgcgttat aacatacatg
    11521 taagcggtac ccaaaatggt gccaagtcaa aatttttaag gaggaaaata catgtcttca
    11581 catccaattc aggtcttctc agaaattggg aaactgaaaa aagttatgtt gcaccgtcca
    11641 ggcaaggagt tagaaaactt gttgccggac tatcttgaaa ggcttctttt tgatgatatt
    11701 cctttcttgg aagatgctca aaaagaacat gatgcatttg cccaagctct tcgcgatgaa
    11761 ggaattgagg ttctctacct agaacaactc gctgctgaat cattgacctc tccagaaatc
    11821 cgcgatcaat ttatcgagga atacttagac gaagccaaca tccgtgatcg tcaaaccaag
    11881 gttgctattc gtgaattgct tcacggcatc aaggacaacc aagaattggt tgaaaaaaca
    11941 atggctggga ttcaaaaagt tgaattgcca gaaattcctg acgaagctaa agatctaact
    12001 gacttagttg aatcagatta tccatttgca attgacccga tgccaaacct ctatttcact
    12061 cgcgacccat ttgcaacaat tggaaacgcc gtatcgctta accacatgtt tgcagacact
    12121 cgtaaccgtg aaacactcta cggtaagtat atcttcaaat accacccaat ctatggcgga
    12181 aaagtggatt tggtctacaa ccgtgaagaa gatacgcgta tcgaaggtgg agacgagcta
    12241 gttctttcta aagacgtcct tgcagtaggt atctctcaac gtacagacgc agcttctatc
    12301 gaaaaacttt tggtcaacat cttcaagaaa aatgttggct tcaagaaagt tttggccttt
    12361 gaatttgcta acaaccgtaa attcatgcac ttggatactg tcttcactat ggtagactat
    12421 gacaagttca ctattcaccc agaaatcgaa ggcgaccttc acgtttactc agttacttac
    12481 gaaaacgaaa aacttaaaat cgttgaagag aaaggtgact tagctgaact tcttgctcaa
    12541 aaccttggtg tagaaaaagt tcatttgatt cgttgcggtg gtggcaatat cgtagcagct
    12601 gcgcgtgaac aatggaacga cggttctaac actttgacca tcgcacctgg tgtggtagtt
    12661 gtttatgacc gcaataccgt gaccaataag attttggaag aatacgggct tcgcttgatt
    12721 aagattcgcg gaagtgaatt ggttcggggc cgtggtggac ctcgttgtat gtctatgcca
    12781 tttgaacgtg aagaagtgta atcgctgttc ggcattcgtc aatagaaaat gtaaaaaata
    12841 gaaagaggaa ataataaaat gacaaattca gtattccaag gacgcagctt cttagcagaa
    12901 aaagacttta cccgtgcaga gttagaatac cttattggtc tttcagctca cttgaaagat
    12961 ttgaaaaaac gcaatattca acaccactac cttgctggca agaatatcgc tctcctattt
    13021 gaaaaaacat ctactcgtac tcgtgcagcc tttacaactg cggctatcga ccttggtgct
    13081 cacccagaat acctcggagc aaatgatatt cagttgggta aaaaagaatc tactgaagat
    13141 actgctaaag tattgggacg tatgtttgac gggattgaat tccgcggatt cagccaacgt
    13201 atggttgaag aattggcaga attctcaggc gttccagtat ggaacggtct aactgacgaa
    13261 tggcacccaa ctcaaatgct cgctgactac ttgactgttc aagaaaactt cggtcgcttg
    13321 gaaggcttga cattggtata ctgtggtgat ggacgtaaca acgttgccaa cagcttgctc
    13381 gtaacaggtg ctatccttgg tgtcaatgtt cacatcttct caccaaaaga actcttccca
    13441 gaaaaagaaa tcgttgaatt ggcagaagga tttgctaaag aaagtggcgc acatgttctc
    13501 atcactgaag atgctgatga agcagttaaa gatgcagacg ttctttacac agacgtttgg
    13561 gtatcaatgg gtgaagaaga caaattcgca gaacgtgtag ctcttcttaa accttaccaa
    13621 gtcaatatgg acttagttaa aaaagcaggc aatgaaaact tgatcttcct acactgcttg
    13681 ccagcattcc acgatactca cactgtttat ggtaaagacg ttgctgaaaa atttggtgta
    13741 gaagaaatgg aagtaacaga cgaagtcttc cgcagcaagt acgctcgcca cttcgatcaa
    13801 gcagaaaacc gtatgcacac tatcaaagct gttatggctg ctacacttgg taacctttat
    13861 attcctaaag tataatttta gataataaac cgtctaccaa cagctatgag ggctgcgact
    13921 aatagcttta gtccggtcct cttttatgta atggtaatct attatttctt ataaaatatg
    13981 tgaaaaatca ttaaattgaa atctaaacgc attctattga gtgtgataaa ggagaattta
    14041 tggcaaatcg taaaattgta gtagctttgg gaggaaatgc gattctttct tctgacccat
    14101 cagcaaaggc tcaacaagaa gctttagttg aaacagctaa gcatcttgta aaattgataa
    14161 aaaatggaga tgatctgatt atcactcacg gtaatggacc tcaagttggg aatctcttgc
    14221 tccaacattt ggcatcagac tctgaaaaga accctgcctt cccactcgac tcacttgtcg
    14281 ctatgacaga aggtagcatc ggtttctggt tgaaaaatgc tttgcaaaat gctctcttgg
    14341 atgaaggcat cgaaaaaaat gttgcctctg ttgtaacgca agttgtcgta gataaaaatg
    14401 atccagcttt tgttaacttg agtaaaccaa tcggtccttt ctattcagaa gaagaagcaa
    14461 aagcagaagc cgaaaagagc ggagcgactt tcaaggaaga tgctggccgt ggctggcgta
    14521 aggtcgttgc ctcaccaaaa cctgttgaca tcaaagaaat tgaaaccatc cgtactcttt
    14581 taaataatgg tcaagtcgtc gtagctgcag gtggtggcgg tattcccgtc gtcaaagaaa
    14641 acaatggaca tttgactggt gtcgaagcgg ttattgataa agacttcgct tcccaacgtt
    14701 tggcagaatt ggttgatgca gacctcttca tcgttttgac aggtgtagat tatgtatttg
    14761 ttaactacaa caagccaaac caggaaaaat tggaacatgt gaatgttgcc cagctagaag
    14821 aatatatcaa acaagatcag tttgcaccag gtagcatgct tccaaaagta gaagcagcta
    14881 tcgcttttgt caatggtcgt ccagaaggaa aagcagttat tacttccctt gaaaatctag
    14941 gcgccttgat tgaatctgaa agcggaacaa ttattgaaaa aggataagtt gttttactaa
    15001 taagatgtat tctatttcta gtatctttat atcaaattag aaattattct tgaaaacatg
    15061 tacaatattt caaaagatac tagttttaga ctttaatatg gtaaaacaaa tataaataga
    15121 aagcgttttc ttaaatgttt atttaagaaa gtagttggtt ttttacactt tgttagacat
    15181 caggaggaaa aacaaatgag tgaaaaagct aaaaaagggt ttaagatgcc ttcatcttac
    15241 accgtattat tgataatcat tgctattatg gcagtgctaa cttggtttat ccctgcgggg
    15301 gcctttatag aaggtattta cgagactcag cctcaaaatc cacaagggat ttgggatgtc
    15361 ctcatggcac cgattcgggc tatgctaggt actcatccag aggaaggttc gctcattaaa
    15421 gaaacgagcg cagcgattga tgtagccttc ttcatcctta tggttggtgg tttccttggc
    15481 attgtcaaca aaactggtgc tcttgacgta gggattgcct ctatcgtgaa gaagtataag
    15541 ggccgcgaaa aaatgttaat tttggtactg atgcctttgt ttgccctcgg tggtacaact
    15601 tatggtatgg gtgaagaaac aatggccttc tatccactcc ttgtgccagt tatgatggcc
    15661 gttggttttg atagcctgac tggtgttgca attattttgc tcggttctca aatcggctgt
    15721 ttggcatcta ctctgaatcc atttgcgaca ggtattgctt cagcgactgc gggagttggt
    15781 acaggggacg gtatcgtact tcgtctgatc ttctgggtta ccttgactgc tcttagtact
    15841 tggtttgttt accgttatgc ggataagatt caaaaagatc cgactaagtc actggtttat
    15901 agtactcgca aagaagattt gaaacacttt aacgtagaag aatcttcatc tgtagaatct
    15961 acacttagca gcaaacaaaa atcagttctc ttcttatttg tgttgacatt catcttgatg
    16021 gtattgagct tcattccatg gacagacctt ggcgttacca tttttgatga ctttaatgct
    16081 tggttgactg gtcttccagt tattggtaat attgtcggtt catctacttc tgcactaggt
    16141 acttggtact tcccagaagg cgcaatgctc tttgccttta tgggtatcct gattggtgtt
    16201 atttatggtc ttaaagaaga taagattatc tcttccttca tgaatggtgc tgctgacttg
    16261 ctcagtgttg ccttgatcgt agcgattgct cgtggtattc aagttatcat gaacgacggt
    16321 atgattaccg atacaatcct caactggggt aaagaaggct tgagcggtct atcttcacaa
    16381 gtctttatcg ttgtaactta tatcttctat ctacctatgt cattcttgat cccatcttca
    16441 tctggtcttg ccagcgcaac tatgggtatc atggctccac ttggagaatt tgtaaatgtc
    16501 cgtcctagct tgattatcac tgcttaccaa tctgcttcag gtgtcttgaa cttgattgca
    16561 ccaacatctg gtattgtgat gggagctctt gcacttggac gtatcaacat tggtacttgg
    16621 tggaaattca tgggcaaact cgtagtcgct attattgtag tgaccatcgc ccttcttctc
    16681 cttggaacct tccttccatt cctataaaat agtgagtgag gtgattccat gaaaatagat
    16741 ataacaaatc aagttaaaga tgaatttctt atatcattaa aaaccttgat ttcctatcct
    16801 tcagtactca atgaaggaga aaatggaaca ccttttggac aagcaatcca aaatgtccta
    16861 gaaaaaactt tagagatttg tcgagacata ggtttcacta cctatcttga ccctaaaggt
    16921 tattacggat atgcagaaat cggtcaggga gcagagcttc tggccattct ctgtcatttg
    16981 gatgttgttc catcaggtga tgaagcagat tggcagacac cgccatttga agcaactatc
    17041 aaagacggct gggtattcgg acgtggtgtc caagatgata aaggcccttc gctcgcagct
    17101 ctctatgcag taaaaagctt gctggaccaa ggtattcagt tcaaaaagtg cgtacgcttt
    17161 atctttggta ccgatgagga aaccctctgg cgctgcatgg cacgctacaa taccatcgaa
    17221 gaacaggcca gtatgggctt tgcacctgac tcatcttttc ctctgaccta tgctgaaaaa
    17281 gggcttctac aagtcaaact tcatggccct ggatcagatc aactagagct tgaagtagga
    17341 ggcgccttta acgttgtacc agacaaggcc aactaccaag gtcccctcta tgaacaggtt
    17401 tgtaacgatc tcaaagaagc tggttatgat taccaatcca ctgaacaaac cgtaacggtt
    17461 ctcggagtgc caaagcatgc taaggatgct agtcaaggta tcaatgctgt catccgacta
    17521 gctaccattc ttgctcctct ccaagaacac cctgctctca gctttcttgc aacacaagca
    17581 ggtcaagacg gcacaggaag acaaatcttt ggtgatatag cagatgaacc ttctggtcac
    17641 ctatccttta atgtcgcagg tctcatgatc aatcatgaac gttctgaaat ccgtattgac
    17701 attcggactc ctgtcttagc tgataaggaa gaactagtag agttgcttac aagatgtgca
    17761 caaaactacc aactccgcta cgaagagttt gactatctag cgcctctata cgtcgcaaaa
    17821 gacagtaaac tcgttagcac actgatgcaa atctaccaag aaaagactgg cgataacagt
    17881 cctgctattt catccggtgg tgccactttt gctcgcacca tgccaaattg tgtagccttc
    17941 ggcgccttat tcccaggagc gaagcagaca gaacatcagg caaatgaatg tgccgttcta
    18001 gaagatttgt accgtgctat ggatatttat gccgaagccg tctatcgact tgcaacttaa
    18061 tcaggcaact gtttctacca aagaaaatcg accgattaat gaactgcacc ccaaaagtta
    18121 gacagaataa atctaacttt tggggtgttt tattatgaaa ttgagttatg aagataaagt
    18181 tcagatctat gaactaagaa agcaaggaca aagcttcaaa cagctttcaa aaagatttgt
    18241 gtgggtgttt cgataaactc aaacttttta gccgtggtat tgcctgattt tatagtatat
    18301 tgaaactaga atagtacacc tctacttcta aaacattttt agaaatcgat ttgactgtcc
    18361 tgaacgattt gtcctgttct tatttcattt tactatattt gagccacttc gtctttaacg
    18421 gctttattca taagctcttg taatttttct ttactatcaa ttacttctga ttttccgttg
    18481 taatttattg taataggttt taacttacct aatttctcga cacgctcatt aatttgatct
    18541 tttttgaagg ctgcatatgt ttttcctaat attttttcga aaatatattt atcagatagc
    18601 ggtttgcctt cttcttcagc ttggtttttg tattgatttg aaacataagg aacaaatgct
    18661 tcatagtaac ctaatactcc cataagttca aaagcttgtt ttctaattca aaccattgca
    18721 aatcagattt cagcttttca gataaatcct gctcatccaa ataatgactt aaaattagtg
    18781 ctgaactcgt ttctgtatcc tgtacaggct gatcacccat accagcaaaa aataaactcg
    18841 ttcctagcaa gacctaacaa gctcctattg catatggcct caaagaaaaa cgcggctttc
    18901 tctcaaattg aaattctttc atcccatctc ccatcattca ttattactgt atattttgta
    18961 tatcagaaat agtttgtatt cacaaatctt tctagttatt cccttatcat tcctaattaa
    19021 gggagataac atacaataat ttttagttaa atgtatatcg atgttttttg tttttcttaa
    19081 taaacgcaat acaaaaagag cctgttgcca agctctttgt actcaatgaa aatcaaagag
    19141 caaactagga aactagccac aggttgctca aaacaccgtt ttgaggtagt agataa
//
LOCUS       DAVBCN010000037        19131 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVBCN010000037 DAVBCN010000000
VERSION     DAVBCN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19131
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.037"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            16..339
                     /locus_tag="VQ497_001187"
                     /pseudo
     CDS             16..339
                     /locus_tag="VQ497_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            257..685
                     /locus_tag="VQ497_001188"
     CDS             257..685
                     /locus_tag="VQ497_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET2517498.1"
                     /translation="MSKSTSKRYYQVAIPFTRLFCLVLVSIDYINCLSETADKNWHKS
                     DRIFVTNTGKTVHSSILSKSLQRANERLKKPIPKHLSPHIFIHITISILSENKIPLKT
                     ITDRVGHPDSEVTNSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(1177..1587)
                     /locus_tag="VQ497_001189"
     CDS             complement(1177..1587)
                     /locus_tag="VQ497_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517499.1"
                     /translation="MPKKKIERISVIHREKILWLKWYFMRDKEKPKYSVLECKMFDAA
                     KNKDMLAYKKYATIKQITDIRVQTSEDDILTAIKEVYVYNHMNVIGACQRILFVSQSP
                     AYNKLNKWFETYSDLYFSIIPLPNMGAYHELVDS"
     gene            complement(1605..1982)
                     /locus_tag="VQ497_001190"
     CDS             complement(1605..1982)
                     /locus_tag="VQ497_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517500.1"
                     /translation="MRKYLKEIKELQELKELLSSRNTPEVIIVEGNDDLGEFFQVDGE
                     LFSDIELLENLKKWREWEVQVIVDDWCNRGLNEYETGILYFPKHEDKMDYIRFNKGLE
                     PLYHALDEPYTTISKSEWLKLLD"
     gene            complement(1924..2352)
                     /locus_tag="VQ497_001191"
     CDS             complement(1924..2352)
                     /locus_tag="VQ497_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HET2517501.1"
                     /translation="MAGNENDGLTSKQIKFIDAMLTEPTIDKACQKAGVSRATGHKYL
                     KVAAVKKTLRLKQDEMMDKTTQMLYLASSNAVSVLNDIMMDAKINPFIRTQAAKTILE
                     QSYKTHEIFGVVRQIEELRLEIEEVSKGDQRVTRTQGIIK"
     gene            complement(2396..3001)
                     /locus_tag="VQ497_001192"
     CDS             complement(2396..3001)
                     /locus_tag="VQ497_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517502.1"
                     /translation="MTFTTIKDDIKAFGKKKHGYMTGYIAKQEELHKQLQNGMIGEKY
                     AKDQLEAYKLEGDTYSRDTYNKIHAEIEKQHDLELEALKEKELSVTADDVAELTLLAS
                     MKMTKDELLGYFEKYKNKPLAIKKLWSIAEQYPEIAINLELFNAEQALESLILFFKRQ
                     LSYCHYSLLINGDKIQAVTTEMVVNSDAPELDRRLDEYLNK"
     gene            complement(3091..3264)
                     /locus_tag="VQ497_001193"
     CDS             complement(3091..3264)
                     /locus_tag="VQ497_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517503.1"
                     /translation="MTENKDNKLLEMMEKGFVLYSKNGIIKYIEIPDHGSIKLKAQDG
                     QLVYKEVTTGEQC"
     gene            complement(3863..4759)
                     /locus_tag="VQ497_001194"
     CDS             complement(3863..4759)
                     /locus_tag="VQ497_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET2517504.1"
                     /translation="MKSLQSSLGNIETFKLDELCPKHEIQLTQIKGERHVVVGWNENG
                     EVIKEVRCIPPYCEQCQEEQKKQDEEDAIADNLNAKLYLQTYNVLMSNKSYVADEFKT
                     KTFDDFNAVTSEEKRFLEFAKGQVQKYLDGMRGNTLITGGTGIGKTLLSVAIAKGINE
                     GYKTKGEPKSVLFVSLTEMIKQIKEGWNYGKGATLTEFEATELMKSVDYLIIDDLGAK
                     NAVIKPKSDWEQDLLFDVLNNRENTIFNTNLDSDELKIVYNERNYSRILKGLEGNTFK
                     AFTIKDKRYSINQLKNKVKQLK"
     gene            complement(4771..5580)
                     /locus_tag="VQ497_001195"
     CDS             complement(4771..5580)
                     /locus_tag="VQ497_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HET2517505.1"
                     /translation="MAKTKVYFWLKIDKKFFDNIFIKRLKTVPGGYTMTVIYIRLMLE
                     SLESDCILYYEGYFNNLKEELALKLDVSEDDIDMTMAYFTKCGLIQIDEDKNAELPQA
                     KAMVMSETNWASYKREQRKKGKLEEVQPSLTFSNSCPTEIEKELEIEVDKEQSPTPST
                     INQDFVNLYKSFEAETGKALSPLQIQELQYMLEDFSPELIHEALKEAVSQGKANFAYI
                     KAILNRWKQDNLLTVELVRNSRAAREAKKQQTNQLEPTSYEDWIPTQEKPF"
     gene            complement(5655..5870)
                     /locus_tag="VQ497_001196"
     CDS             complement(5655..5870)
                     /locus_tag="VQ497_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517506.1"
                     /translation="MNELDISNTQAVFVTVILIGLLLYLNHRDRKKSAQLERENQQTI
                     ETPSEDLNPDYGRYIQLAGVDVWGRIE"
     gene            complement(5872..6072)
                     /locus_tag="VQ497_001197"
     CDS             complement(5872..6072)
                     /locus_tag="VQ497_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517507.1"
                     /translation="MTLDLDNMTQAEFDEAMAEIQLQSPNIFQLISDFVNKKVTSVEI
                     DDLLNMKRAEQVAYIKNYKARA"
     gene            complement(6069..6260)
                     /locus_tag="VQ497_001198"
                     /pseudo
     CDS             complement(6069..6260)
                     /locus_tag="VQ497_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841301.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6249..6539)
                     /locus_tag="VQ497_001199"
                     /pseudo
     CDS             complement(6249..6539)
                     /locus_tag="VQ497_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            complement(6709..6861)
                     /locus_tag="VQ497_001200"
     CDS             complement(6709..6861)
                     /locus_tag="VQ497_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517508.1"
                     /translation="MKIVTFKPTKQIDGGFYLPGIDILFVSDKADAKSTEDVILFISR
                     NGLNKS"
     gene            complement(6872..7138)
                     /locus_tag="VQ497_001201"
     CDS             complement(6872..7138)
                     /locus_tag="VQ497_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517509.1"
                     /translation="MEILSKEIQLQGLQLLKQTLETLVELEKQRSSKLDLISRKELMD
                     LLGISATTLDNWEDLALKRYQTPMDGAKKVFYRLSDVYLFLAIK"
     gene            complement(7349..7531)
                     /locus_tag="VQ497_001202"
     CDS             complement(7349..7531)
                     /locus_tag="VQ497_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2517510.1"
                     /translation="MLITLSLAEKVRIKRARLQLTKKAVSEQLGIKSQTLTKVENGNY
                     DASKRIYESVMTWLVE"
     gene            7593..8096
                     /locus_tag="VQ497_001203"
     CDS             7593..8096
                     /locus_tag="VQ497_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2517511.1"
                     /translation="MYKMNRIKQLRKENNLTQRELANETKIPYRTIQRWENGETDIKS
                     DAAQVLADYFGVSIPHLLGYSDLIADTDSDIKAMAYSHLLTFVDEKQIKEFEKEFIRA
                     NDNSDTFGDLGSGEKALEKYYQALCYLVPPFRDILARYSFLSLDEKKSVYNIIVGLSN
                     NLSKENK"
     gene            8170..9333
                     /locus_tag="VQ497_001204"
     CDS             8170..9333
                     /locus_tag="VQ497_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET2517512.1"
                     /translation="MNIIEKIKKNGQRVYYASVYLGVDQLTGKKARTTVTATTKKGVK
                     VKARDAINTFAANGYTVKDKPTITTYNELVKVWWDSYKNTVKPNTRQSMDGLVRVHLL
                     PVFGDYKLSKLTTPILQQQVNKWADKANKGEKGAFANYSLLHNMNKRILKYGVAIQVI
                     QYNPANDVIVPRKQQKEKAAVKYLDNKELKQFLDYLDTLNQSNYENLFDVVLYKTLLA
                     TGCRISEALALEWSDIDLESGVISINKTLNRYQEINSPKSSAGYRDIPIDKATLLLLK
                     QYKNRQQIQSWKLGRSETVVFSVFTEKYAYACNLRKRLNKHFDAAGVTNVSFHGFRHT
                     HTTMMLYAQASPKDVQYRLGHSNLMITENTYWHTNQENAKKAVSNYETAISNL"
     gene            complement(9487..10791)
                     /gene="eno"
                     /locus_tag="VQ497_001205"
     CDS             complement(9487..10791)
                     /gene="eno"
                     /locus_tag="VQ497_001205"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET2517513.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            10955..11401
                     /locus_tag="VQ497_001206"
     CDS             10955..11401
                     /locus_tag="VQ497_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HET2517514.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(11398..12513)
                     /locus_tag="VQ497_001207"
     CDS             complement(11398..12513)
                     /locus_tag="VQ497_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET2517515.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIDVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGRHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(12522..12851)
                     /locus_tag="VQ497_001208"
     CDS             complement(12522..12851)
                     /locus_tag="VQ497_001208"
                     /inference="COORDINATES: protein motif:HMM:NF019887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HET2517516.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGGNNLLMLKSAELGIAFCSKEMLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(12805..13167)
                     /locus_tag="VQ497_001209"
     CDS             complement(12805..13167)
                     /locus_tag="VQ497_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HET2517517.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHC"
     gene            complement(13226..14659)
                     /gene="glgA"
                     /locus_tag="VQ497_001210"
     CDS             complement(13226..14659)
                     /gene="glgA"
                     /locus_tag="VQ497_001210"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HET2517518.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(14656..15795)
                     /gene="glgD"
                     /locus_tag="VQ497_001211"
     CDS             complement(14656..15795)
                     /gene="glgD"
                     /locus_tag="VQ497_001211"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HET2517519.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVRTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(15785..16927)
                     /locus_tag="VQ497_001212"
     CDS             complement(15785..16927)
                     /locus_tag="VQ497_001212"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET2517520.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(16917..18845)
                     /gene="glgB"
                     /locus_tag="VQ497_001213"
     CDS             complement(16917..18845)
                     /gene="glgB"
                     /locus_tag="VQ497_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HET2517521.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEYGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAIVTELPDKKWRDGLWLARRKRWGFAERPVNIYEVHAGSWKRNPDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDIYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEVIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         5683 a         4000 c         3226 g         6222 t
ORIGIN      
        1 ttgcaggtct aacaaatgaa tggtgagtta atcgctccaa tgacttacga agagatgatg
       61 acgagcgact tttttgaagc ttggtttcag aagtttctct taccaacatt aaccacacca
      121 tcggttatta ttatggataa tgtaagattc catagaatgg ggaagctaga actcttgtgt
      181 gaatagtttg ggcataaact tttacctctt cctccctact cacctgagta caatcctatt
      241 gagaaaacat gggctcatat caaaaagcac ctcaaaaagg tattaccaag ttgcaatacc
      301 ttttacgagg ctcttttgtc ttgttcttgt ttcaattgac tatataaatt gtctaagcga
      361 aacagccgat aagaattggc acaaaagcga ccgtattttt gttaccaata caggaaaaac
      421 agttcatagt tctatcttga gcaagtctct ccagcgagca aacgaacgcc ttaaaaaacc
      481 aattcccaaa catctgtccc ctcacatctt catacacatc actattagca tcttatcaga
      541 aaataaaatt cctttaaaaa caatcacgga cagggttggt catcccgact ctgaagtcac
      601 taattccatc tacacccacg tcacaaagaa catgaaagat gaagcaatca atgtactgga
      661 taaagttatg aaaaagattt tttaaaaagt tttgtccctt ttttgccctc taaatacaaa
      721 aatagccctt cggataaaat ccgaggggct agaaacgttg ttaaatcaac ggccgaactt
      781 ttgaatttca tggttcggga taaaatagtt cactgaacta ttttattttt taaggttatc
      841 ataatatcaa atagttcaat taaatacgct aaattactaa tatacttttt acctttttca
      901 ttctaaaatg taaagtacaa acaattacaa tatactagag ggggagtaaa aaaggtaata
      961 aaattgacga gttcagcagg caagggaaat agcacttttc atggtgttat ttttttaatt
     1021 cagcaatgga gtagccgctt aattgataga aacaaaaagc acctagtttc ctagatgcta
     1081 gcacaatagc ccggattcga accggaatct cctcaattaa ggtgcaatcc ctatatacta
     1141 ctcattgtgc tttggttcat tatatcatac aatcaactag ctatctacca actcatgata
     1201 cgctcccata ttcggtaatg gaataatact aaaatacaaa tctgagtaag tttcaaacca
     1261 tttattcagc ttgttatagg ctggcgactg actaacaaat aatatccgtt gacaagctcc
     1321 gataacattc atgtgattat acacatagac ctctttaatg gccgttaaaa tatcgtcttc
     1381 acttgtctgt accctaatat ctgttatctg cttaatcgta gcgtattttt tataagctag
     1441 catatcttta tttttagcag catcaaacat tttacactca agaacactat acttaggttt
     1501 ttctttatct ctcatgaaat accacttgag ccataaaatt ttttctctgt gaattactga
     1561 aatacgttcg atttttttct ttggcatgat tacctcctaa attattaatc taatagcttt
     1621 aaccactcac tttttgagat tgttgtataa ggttcatcta gcgcgtgata taaaggttct
     1681 aaacctttgt taaatcggat atagtccatt ttatcctcat gctttggaaa atataagatt
     1741 cctgtttcat attcatttag accacggtta caccaatcat caacgataac ctgcacttcc
     1801 cattcacgcc actttttaag gttttctaaa agttcaatat cactaaataa ctcaccatca
     1861 acttggaaaa attctcccag atcatcgtta ccctcgacga tgataacttc aggcgtattt
     1921 ctactactta ataattcctt gagttcttgt aactctttga tctcctttag atacttcctc
     1981 aatttctaac ctcaattctt caatttgtct tactactcca aaaatttcat gagttttata
     2041 tgattgttca agtatagttt ttgctgcttg agttcttata aaagggttaa ttttggcatc
     2101 catcataata tcattgagta cagaaacagc attggatgag gctagatata gcatctgtgt
     2161 agttttatcc atcatctcat cttgttttag tctcaatgtc tttttaacag ctgcaacttt
     2221 tagatactta tgccctgttg cccttgacac ccctgctttt tggcacgctt tatctatcgt
     2281 tggctcagta agcatggcat ctatgaattt tatttgttta gatgtaaggc catcattttc
     2341 atttcctgcc atctattacc tcctcgttat caaaataaag gggttgctag cccctctatt
     2401 tgtttaaata ctcatccaaa cgacggtcaa gctcaggggc atctgaattt actaccattt
     2461 cagttgttac agcttggatt ttatcgccgt tgataagcaa actgtaatga caatatgaaa
     2521 gttgccgctt aaagaataag attagacttt ctagcgcctg ctctgcattg aataattcca
     2581 agttgatagc aatttctgga tattgttcag caattgacca aagtttttta atagcaagag
     2641 gtttgttctt gtatttttca aaataaccta gcaattcatc ctttgtcatc ttcatactag
     2701 ctaacaaggt cagctctgcc acgtcatcag ctgttacgct gagttctttt tcttttaatg
     2761 cctctagctc tagatcatgc tgtttttcaa tttctgcatg aattttatta taggtgtccc
     2821 tagaataagt gtccccctct aacttataag cctctagctg gtctttagcg tatttttcgc
     2881 ctatcatacc attttgtaac tgtttatgta attcctcttg tttagcaatg tagccagtca
     2941 tgtacccatg ctttttcttg ccaaaagcct taatatcatc cttgattgtt gtaaatgtca
     3001 tgttatcctc cttacgaaaa aataaaagag gtatgacaaa agagcgttaa actcaattat
     3061 catacctctg gtttttccag tcagtattta ttaacattgt tctccagtgg tcacttcctt
     3121 ataaactaac tgcccatctt gagcttttag cttaatgcta ccatgatctg gaatttcaat
     3181 gtactttatt ataccatttt ttgagtataa aacaaagcct ttctccatca tttctaataa
     3241 tttattatcc ttgttttcag tcatgaattt ctcctttatt tcaagtgtcc caacgggtac
     3301 ggttgtaagc tgagacattt tagggctatc tttgagcgac aaaaatgtcg aaaagtcttc
     3361 agttcctgta taaccgttat tagttttgtt tgctactcat ttttgagtca caaatctgtt
     3421 aagcaaaaat gagtaatagc tcgtaagata agtaaatcta atacttgtat tcctaaagcg
     3481 ttgatataac tgatattttc aactatccaa cgctctttac tacccttttt agtgtgcttt
     3541 agctcatttt tgagcttttt tgagcttttt tgagttccat tttgggaatg caaagcaggc
     3601 gcgcaccgtg catgcttttt tcttgctcat agcctcaccc tcagaagttt gccgaccgag
     3661 agcgtggggc tttttatttt tgtataatta aaaacagctg cacaattttt gtacagctac
     3721 gaccgaggca ggggcgacct gccgtacttt acactagata atatcatcta ggtagtccaa
     3781 ctgtatttta tcatattaaa ggctacatta caatttctca ccctgtgaag agtgtctatt
     3841 tttgtttagg tttgtaagtc gattacttca actgctttac cttatttttt aactgattaa
     3901 ttgagtatcg cttatctttg attgtgaaag ccttaaaagt gttaccctca agacctttta
     3961 gaatacggct ataattccgc tcgttgtata ctatcttgag ttcatcgcta tcaagatttg
     4021 tattaaatat agtattttct cgattattca gtacatcaaa aagtaaatcc tgctcccaat
     4081 cgctcttagg tttaataaca gcattttttg cacctaaatc atcaataatt aagtaatcaa
     4141 cagacttcat gagttcagta gcttcaaact ctgtaagtgt tgcaccttta ccataattcc
     4201 acccctcttt aatttgtttg atcatttcgg ttaggcttac aaaaagcaca ctcttaggtt
     4261 ctccttttgt cttatacccc tcatttatac ctttggcaat agcaactgat aaaagtgttt
     4321 ttccaatccc tgtacctcct gtgataagcg tatttcccct catgccatca agatattttt
     4381 gtacctgacc ttttgcaaat tctaaaaatc gcttttcttc tgatgttaca gcattaaaat
     4441 catcaaaagt tttagtttta aactcatctg ctacatagct cttattgctc atcaacacat
     4501 tataagtttg cagatatagt ttagcattca aattatcagc aatcgcatct tcttcatctt
     4561 gctttttctg ttcttcttgg cattgttcac aatagggtgg gatacagcga acttctttta
     4621 ttacctctcc gttctcattc caccccacta ctacatgtct ttctcctttg atttgtgtta
     4681 gctgtatttc atgcttagga cacaattcgt ctagtttaaa tgtctcaata tttcctaaac
     4741 tagattgtaa tgatttcatt ttctgacctc ctaaaatggt ttttcttgtg ttggtatcca
     4801 atcttcatag ctggtaggct ctagttgatt ggtttgctgt tttttagcct cacgcgctgc
     4861 cctgctattt ctaacaagtt ccaccgtcaa taaattgtcc tgtttccaac ggttaaggat
     4921 tgccttgatg tatgcaaagt ttgctttacc ctgactgaca gcctctttta acgcctcatg
     4981 gataagctct gggctaaaat cttctagcat atactgcaat tcttgaatct gtaacggtga
     5041 caatgcttta cctgtctcag cttcaaaaga tttatagaga tttacaaaat cttgattgat
     5101 ggtagaggga gtgggtgatt gttctttatc tacttctatc tctaattctt tctctatctc
     5161 tgttggacat gagttggaaa atgtcaaaga tggttggact tcttccaatt ttcctttttt
     5221 tcgttgttca cgtttataac tcgcccagtt tgtttcactc ataaccatag cttttgcctg
     5281 tggtagttct gcgtttttat cttcgtcaat ctgtattaaa ccgcattttg taaagtatgc
     5341 catggtcata tctatatcat cttctgatac atctaatttt agggctaact cttccttgag
     5401 attgttaaag tagccctcat agtataaaat acagtcactt tccaagctct ctaacatgag
     5461 cctaatataa ataactgtca tagtgtaccc accaggtacc gtctttagtc tcttgataaa
     5521 aatattatca aaaaatttct tatcaatttt tagccaaaaa tagacttttg tttttgccat
     5581 cgtcctctcc cttgctcatt tataattctc taccttgatt agtcttaata ctcgcttgtc
     5641 attcattgtt agactcattc aattcttccc catacgtcta cccctgcaag ctggatatat
     5701 cgcccataat cagggtttaa atcctcgcta ggtgtttcta tcgtctgttg gttttctcgc
     5761 tccagttggg cgcttttttt gcggtctcgg tggtttaaat aaagcagtaa gccaatcaaa
     5821 atcacggtaa caaaaaccgc ctgtgtattg cttatatcta gttcattcat gttatgccct
     5881 cgctttgtaa ttcttgatgt atgccacttg ttcagctcgc ttcatattca acaagtcgtc
     5941 tatttccaca ctggttactt ttttatttac aaaatcagaa atcagttgaa aaatgtttgg
     6001 actttgtagc tggatttcag ccattgcctc atcaaattct gcttgtgtca tgttgtctaa
     6061 atctagtgtc attgcattgc ctcctcaaac ttctctataa gacaactttt atttgctttc
     6121 tgagttccat ttttagagtt aaaaagaata tattttaagg ttacagtagc ctctaaatac
     6181 tccttttcag catgctctat atacgcctgt tgctctgctt cgttctcaaa aaagtgctta
     6241 gcttagcgtt taaagaatgc tttgactagg tttagtttga attgccgtac tgcttcggta
     6301 ttctttagat aagtgatcag caaagtagcc tgttgctcgt tcagaatata ggatttttta
     6361 ggttgtcctc tagtgtctaa tttatggatt ttaaatccaa gtattcccaa ctcttcaaag
     6421 tcaaccttat tttctcttat taagcgtgtg atagtgtggt gttgtacgtc agcacattca
     6481 gcgatgatct cgcttgtggt atacggctct ttcttgccgt ccatgtagac tagttccatt
     6541 atggttctac ctcctgtata aatttagcta gcttactttt taattgtctc ctctagccct
     6601 ttttagcctc tacaacggtt ttggctaacg tgttagaggc ttttataacg gcctgtatag
     6661 ctgtctctat ttcctccgtg gaatagtagc tttgttttct aatatcattc aagacttgtt
     6721 taacccattt cttgaaataa ataaaattac atcttctgta ctcttggcat ctgctttatc
     6781 tgagacaaat agaatgtcaa tgcctggtag ataaaaccca ccgtctattt gtttagttgg
     6841 tttgaaagta actattttca tttcataact cctattttat tgctaaaaat aaatacacat
     6901 ctgacagacg atagaatact ttcttagctc catccatcgg ggtctgatat cgtttaagag
     6961 caagatcctc ccagttatca agggttgtag cacttatacc tagcagatcc attaattctt
     7021 tacgagaaat taaatctaac ttactagatc gttgtttttc tagctcaact aaagtttcaa
     7081 gagtctgttt aagaagctgt aaaccctgta actgtatttc tttagacaga atttccattt
     7141 atatcctcca ttttcctttt tataaattaa gttgtctttt tcctctaatt ttgataaaat
     7201 aagaagagat tatttagcaa agggggttgt ccctttgcta aatacctaac aaaaccacgc
     7261 gccttgcctt tgcttgagtt ttgttatttt tccttagccg tgtacgttgg tttggtcgcc
     7321 tgtacatggc tttttacttg cgttaaattt attctactag ccaagtcatc acgctctcat
     7381 agatacgctt agaggcatca tagtttccat tctcaacttt tgttagtgtt tgagatttaa
     7441 tacccaactg ctccgatact gcttttttgg ttagctgtaa tcttgcacgc ttgatacgta
     7501 ctttttcagc aagtgatagt gtaatcaaca ttggtttcac ctcccttttt tctccatttt
     7561 tggcattttg tgttataatc tctttagaaa ggatgtataa gatgaataga attaagcaat
     7621 tacgaaaaga aaataatctt actcaaagag aacttgctaa tgagactaaa atcccttata
     7681 gaaccataca acgttgggag aatggggaaa ctgatataaa atccgatgct gctcaggtac
     7741 ttgctgacta ttttggagta tcaattcctc acttactagg ttatagcgat ttgatagcag
     7801 atacagatag tgatattaaa gcaatggcat actcacatct actaactttt gtagatgaaa
     7861 aacaaatcaa agaatttgaa aaagagttca ttcgtgcaaa cgataatagt gatacttttg
     7921 gtgatttagg aagtggtgaa aaggcgttag aaaaatacta tcaagcatta tgctatttag
     7981 ttcctccttt tagggatata ttggctaggt atagcttttt gtcactagat gaaaaaaaat
     8041 cagtctataa tattattgtt ggtttaagta acaacttatc aaaagaaaat aaataacttt
     8101 aaacatcatg tataaatacc taacaaaacc acgcgccttg cctgctgatg gaaagaaagg
     8161 tacaaataca tgaatatcat agagaaaatt aaaaagaatg gccaaagagt ttattatgct
     8221 agtgtttatc taggcgttga ccaactaacg ggcaaaaaag cccgtacaac tgttacagca
     8281 accactaaaa agggcgttaa agtaaaagcg cgtgatgcga tcaatacttt tgctgctaat
     8341 ggctatacag ttaaagataa gccgacaatt acaacataca atgagcttgt aaaagtttgg
     8401 tgggatagtt acaagaatac agttaagcca aatactcgcc aatccatgga tggattggtt
     8461 agagtgcatt tattgcctgt atttggcgat tacaagctat ctaaacttac tacgcctatt
     8521 cttcaacagc aagtaaacaa atgggctgac aaggcaaata aaggcgaaaa aggggcattt
     8581 gctaactact ccttgctcca taacatgaat aagcgtattt tgaaatatgg cgtagctatc
     8641 caggtaatac aatacaaccc agctaatgat gtaatcgttc cacgcaaaca gcagaaagaa
     8701 aaggctgctg tcaaatactt agacaacaaa gaattaaaac agtttcttga ttatttagac
     8761 actctgaatc aatcaaatta tgagaactta tttgatgttg tcctgtataa gactttattg
     8821 gccactggtt gccgtattag tgaggctcta gctcttgaat ggtctgatat tgacctagaa
     8881 agcggtgtta tcagcatcaa taagacacta aaccgctatc aggaaataaa ttcacctaaa
     8941 tcaagcgctg gttatcgtga tataccaata gacaaagcca cattactttt actgaaacaa
     9001 tacaaaaacc gtcaacaaat tcagtcttgg aaattaggcc gatctgaaac agttgtattc
     9061 tctgtattta cggagaaata tgcttatgct tgtaacttac gcaaacgcct aaataagcat
     9121 tttgatgctg ctggagtaac taacgtatca tttcatggtt tccgccatac acatactact
     9181 atgatgctct atgctcaggc tagcccgaaa gatgttcagt atagattagg ccactctaat
     9241 ttaatgatca ctgaaaatac ttactggcat actaaccaag agaatgcaaa aaaagccgtc
     9301 tcaaattatg aaacagctat cagcaattta taaaaaataa gggtgaccca tttccgggct
     9361 accctcttac tataccaaaa attagtaggg gtagtaaaaa gggtattaaa ttataaaaag
     9421 cactaaggga aagcgcccca aagtgcttat ttcaaaggct ttatagccta taatcacata
     9481 aggagattat tttttaaggt tgtagaatga tttcaatcca cgatattcag ctacttcgcc
     9541 aagttggtct tcgatacgaa gcaattggtt gtatttagcg atgcggtctg tacgtgaaag
     9601 tgaaccagtc ttgatttgtc ctgcgttagt tgcaactgca atatcagcga ttgttgaatc
     9661 ttcagtttca cctgaacggt gtgatacaac agcagtgtaa ccagcttctt tagccatttc
     9721 gatagcttca aaagtttcag taagagtacc gatttggtta actttgataa ggattgagtt
     9781 agcagcacct tcttggatac cacgtgcaag gtagtcagtg tttgttacga agaagtcgtc
     9841 accaacaagt tgtactttct taccaagacg ttcagtaaga gctttccaac catcccagtc
     9901 gttttcatcc ataccatctt caatagtgat gattgggtat ttgttaacca attcttcaag
     9961 gtagtcgatt tgttctgcag atgtacgaac agcagcacct tcaccttcaa atttagtgta
    10021 gtcgtaaact ttacgttctt tatcgtagaa ttctgatgaa gcacagtcaa atccgataaa
    10081 tacgtcttta cctggtacat atccagcagc ttcaatcgca gcaaggatag tttcaacacc
    10141 atcttcagtt ccttcgaaac gaggagcgaa tccaccttcg tcacctacgg cagtttccaa
    10201 accacgtgat ttaaggattt tcttaagagc gtggaagatt tcagcaccgt aacgaagggc
    10261 ttctttaaat gttggcgcac caactggcaa gatcatgaac tcttggaaag cgattggagc
    10321 gtcagagtga gaaccaccgt tgatgatgtt catcattgga gttggaagaa ctttagtgtt
    10381 gaatccacca aggtagctgt aaagtgggat ttcaaggtag tcagcagcag cacgagctac
    10441 agcgatagac acaccgagga ttgcattcgc acccaattta cctttgttag gagtaccgtc
    10501 aagtgcgatc atagcacggt caatagcttg ttgatcacgt acatcgtagc caatgatagc
    10561 ttcagcaatg atgttgttta cgttgtcaac agctttttgt gtaccaagac caccgtaacg
    10621 agatttgtca ccgtcgcgaa gttcaactgc ttcgtgttca ccagtagaag ctcctgatgg
    10681 aaccatacca cgtccgaaag cacctgattc agtgtaaact tctacttcaa gtgttgggtt
    10741 accgcgtgag tctaggactt cgcgagcgta aacatcagta ataattgaca ttttttactc
    10801 tccttatgag ttaaattttt tacacctcta taatacctta aaacccctcc tttttcaaga
    10861 aaaaacgtta tctttgtgca acttttcctt aactttataa agtaatcgct ttcttttgtc
    10921 tgttttattc taacttttat gatatactgt tttcatgaca gatttatcaa aacaattact
    10981 tgaaaaagct catggtgggt taaaaataaa tccggatgag caaagacgct atcttggtac
    11041 ttttgaggaa agagttcttg gatatgtaga tattgacaca gcaaatagcc ctcagttaga
    11101 aaaaggcttt ttatttattt tagaaaacct tcaggaaaaa gcagagccac tatttgtgaa
    11161 gatttcacca actatcgaat ttgataagca agttttctac ttaaaagaag caaaagaaac
    11221 tgatagtcaa gccaccatag tatctgaaga gcatattact tctccttttg gcctggttat
    11281 tcatagcaat gcaccagttc aagtagaaga aaaagacctt cgacttgctt ttccaaaact
    11341 ttgggaagtt aaaaaggaag aaccagccaa aacatcctta tggaagaaat ggtttagcta
    11401 aatcttgcac atatttaata agtgcccaat attggcagcc gtgcgctcca gatagagact
    11461 ggcatttttc aaactatctt ctaaaggttc acttttctcc aaaatagaaa agacagcttg
    11521 gatattttca aatggtaggg gaggtaaatc ttcagcaaga ctaccgcaaa tagcaataac
    11581 aggaactcca acaggggttc tttttgcaac acctataggc gctttcccag caaagctttg
    11641 actatcaagt cttccttctc caacaacaac caagtcagca tctgaaactt tcttatcaaa
    11701 gttgattaag tccaagcagg tatcaattcc agacacgata cttgcctgag caaaagcaca
    11761 caaaccacca gcaaggcctc cacctgctcc tgctccttta atttctaatg ttgcaggtga
    11821 gaatttttca taaaaatctt ggatcgcctg atctacgact gcaaacatag tcggatgtcg
    11881 acctttttga ttgccaaaag tataagtcgc accttgatga ccacataagg gacttacgac
    11941 atctgctaaa atatgaattt gaacaccttc aggaatttta tagcaatttt ctgttgaaac
    12001 agaagctaag tttaataagg attgaccgga agcaggcaag acatttccat ccctatcata
    12061 aaattgataa cctaaaccag cagcaatccc cagtcctcca tcattactgg ccgtgccacc
    12121 aacatcgata taaatatctt taatcccttt agaaatgaga tggagaatca actctccaat
    12181 accacaagtt tggatttgaa gtggatttcg tttctctagc ggaatttttc caagaccaac
    12241 caagtcagct acttcaaata gtgccagttc ccctttttga aaatagcgca tggcttcttt
    12301 ttgtccaaaa gggtctgtca cttggatcca tttttctttt aggtcaagag aatgtcggat
    12361 agcatctaca gtaccttctc ccccatcacc aacagggcag aggagacatt ctacatctgc
    12421 tatcgattgt tggaagcctc tttttattgc ttcagctacc tgttgagctg tcaagctttc
    12481 cttaaacgaa tccggtgcaa ttacaatctt catattttcc ctcattctaa acagtcaatc
    12541 aaaggaagaa cttctaaaaa atccctcttg tcaacatgat gtggtatttc ttttttgagc
    12601 atttctttgg aacaaaaggc gattcctaac tccgccgact tcaacattaa tagattattg
    12661 cccccatcac cgattgccac cgttctttct ttagaaagtt ttagtttctt tctccatttt
    12721 tccagagttt cttttttgac ctggggactt ataatttgtc caactaattt tcctgttaaa
    12781 agaccttctt tgacttcaag ctggttagca gtgaaatagg caataccaag ggattttgct
    12841 aatctctcca actattggtg taaatccacc agacaccaga ccaactagga taccattctt
    12901 ttggagaata gagatgaact ctgggacatt tagcgataga tgaattgagt tgaagacgtt
    12961 atcaaagacc aaaataggaa gaccttccaa caaggacact ctttttctta aactgctttc
    13021 aaagaccaac tctcctcgca ttgctcgact tgtaatctgc gaaatttctg cctcatgacc
    13081 tgcctctctc cctaaaagat caatcacttc ttctaggatt aaggttccat ctacatccaa
    13141 aacacacaag ccttttactt gagacatcag ttctcctctc taaacagcct aaaaatcgta
    13201 tgaagtcatc atacgatttt atctattaat taactaaact atggtacaag tcaaggtatg
    13261 acttgcaggc tgtatcccat gagaagtcac tctccatagc ttgtttttgt aggtttctcc
    13321 aaatgtctgg atggtttcta tacaagtcca atgctgtttg gaaagtccaa tttaaccaat
    13381 aaggagatag attgtcaaag ctaaagccag taccgcttcc ttcgattgga ttgaaagcgc
    13441 gaactgtatc tcgcaagcct ccaacttcat ggaccaatgg caaggttcca taacgcatag
    13501 ccatcatttg agacaagcca cacggttcaa aacgacttgg catgaggaag aggtcacaag
    13561 cagcgtagat ttcctgagca agtttgacat caaaagtgat atttgttgat agcttgtctg
    13621 ggtaaatctg agcaaaccat gagaaagctc cttcaaaggc tggatcgcca gttcccaaaa
    13681 gaacaatctg aacatcttct tgcaagatat ggtgaagact ttcgaccacc acatcaaaac
    13741 ctttttgacg tgtcaaacga gaaacaattc ccaccagtgg aacgtctgct ctaacaggca
    13801 agccaactct ttcttgcaat tttgccttat ttttggcttt cccagacaaa tcttcctgat
    13861 tgaaatgata gtctaaaaga gcatccgtct gaggattata aagatcagca tcaatcccat
    13921 tcacgatacc agatacttta ccagactcca ttttaagaat ctgatccaaa ttacatccaa
    13981 actgactagt cataatttca tgagcatagc taggtgaaac ggttgaaaca cggttcgcat
    14041 agagaatacc tgccttcatc cagttcagac agttgttcca tcgaagggtg ccatcagcgt
    14101 aacgttcaaa gccaactcca aacaaatcac ccaacattcc ttctgaaaat tgtccttgga
    14161 attctaaatt atgaatggtt aaaactgttt caatgtcctc ataggcttga atccaacggt
    14221 atttttcctt caacaagaaa ggaatcatag ctgtatggta gtcatgaaca tggagaagat
    14281 caggaataaa gtcaatcctt tccatagcct caatggcagc cagttggaaa aaggcaaagc
    14341 gttctccgtc atcaaaatca ccgtaaacat gaccacggaa gaaataatat tgattgtcaa
    14401 taaagtagaa ggttacacca tttaatactg ttttcttaat tccacaatac tgtctgcgcc
    14461 aaccaacgct cacctcaaaa tgaagcacat cttcaatctg atttccaaat ttagcctcta
    14521 ccatatcata gtagggtaaa atcactgcaa cttcgtgccc agcttttacc agtgattttg
    14581 gaagagcgcc aatgacgtct cccaaaccac ctgtttttga aaagggtgca ccctctgctg
    14641 ctacaaataa aattttcatg aatgaatatc ctctgttact ttagcacctt tcttaaccac
    14701 aactggatgt tctgcagttc ctcgaatcac aacaccaggc tcaacttcaa cccctttgtc
    14761 caagatagca tattcgacct gagccccttc tccaataaca acacgaggga agagcaggct
    14821 atctttaacc aagctatcct tatggacatg gatattacgt gatagaacag aattagctac
    14881 ttgaccttca ataatactac cagaggcaaa ctgagaagtt cttaccttag atgtattagc
    14941 atagtaagtt ggctcttcgt ttttgacctt tgtataaatc ttttggtttg gtgagaaaag
    15001 agaatagaat ttttgtgatt caagcatatc gatattcgct tgataataag atttaacaga
    15061 gtgaatattg gctagatagc ccgtgtactc gtaggcgaaa gctccctctt ttacagccaa
    15121 atcccgtaaa acatagcgca atttctctgg atgttctttt ttagcttctt cttccaagtg
    15181 ttcaatcaac caaggtgtat caacgacaaa gatatctgta gacatattga acgtttcagc
    15241 tgttgacttg ctatcaaaga gtttatgaga aagaacatgg tctgtttcat ctacatccaa
    15301 gattgcattt acttctgaaa tatctttctt agctagtttt ttataaacta cagtgatagg
    15361 ctcttttgtt gtactatgta ggtggaaaac ttggttcaaa tcaatgttaa taagaacatc
    15421 gcagttgagg gcaaccgttt ggtttgagcc agaacgtttc aaataagtaa gaagctgttg
    15481 gtagtattct tttccaactg tactactttc tacacgggta ttgtaaattc ctagatagta
    15541 atggctaaga agggttgata agccccactc gcgtcctgaa cgaatatggt caaatactga
    15601 gctgatatta tcctgctgga aaataccaaa gacactacga acacctgcat tagcaaggct
    15661 tgaaagtggg aagtcaatca aacgatattt cccaccaaat ggcaaacttg ctactggacg
    15721 gtggtccgtc aatgtcgaca tattgtgaaa accaactgta tttcctaaaa tggcagaata
    15781 tttatcaatc ttcatctgtt gctaccccca ctacttcatt atatcctaca acttgtactt
    15841 catctgttcc atcaatttcg acaccgtcag aaataatcgc accttcacca ataatggcac
    15901 gtttaatctt agctccttga ccaatgatag ctccactcat gataactgaa tcaaggactt
    15961 ccgctccttc gcgaacttgc gcgcctgttg aaaggataga atgtttaaca gttccatcaa
    16021 cgaaacatcc gtctacaact aatgagtctt ccacatgagc atttgccccg aggaagtttg
    16081 gtggtgaaat caagtttctt gagtaaatct tccattgacg gttacgacta tccaaggcat
    16141 tttctggaga aatatactcc atgttcgctt cccaaagtga ctcaatagta ccaacatctt
    16201 tccaataacc actaaattcg taagcataaa cactttcacc tgactcaagg taatttggaa
    16261 tgacattttt accaaagtct gacatgccaa ccttgctctt ttcagcagcg actaacatat
    16321 tacgaaggcg ttgccaatca aaaatgtaga ttcccataga agcttttgta gatttaggtt
    16381 gagctggttt ttcttcaaat tcaacaatac gattgttagc atctgtgttc atgataccaa
    16441 aacggcttgc ttctttaaga gggacgtcta aaactgctac tgtcaagctg gcattattat
    16501 ccttatgaga ctggagcata tcatcatagt ccattttgta gatgtgatcc ccagacaaaa
    16561 tcaagacata ctcaggattg acactgtcga tatagtcgat attttggtaa atagcgtgac
    16621 tagtcccctc aaaccaacga tttccttcac ttgcagaata aggttgaaga atagagacac
    16681 ctgaattaat accgtctagt ccccagcttg aaccattccc aatatggttg ttgagagcaa
    16741 gtggttgata ctgtgtaacg accccaacat tatgaatccc tgagttggca cagtttgata
    16801 gggcaaagtc aatgatacgg tagcgcccac caaattgcac agctggtttt gcgatgcttt
    16861 gagtgagttt accgagacga gttccttgcc caccagcaag aatcaaagct aacatttcat
    16921 ttttcatttt ctactccttt ttggttttta tttgtgacgg ttttagtaga tttcaagcga
    16981 cgtttgattt tccatacact tgctcccata gccggtaggg taaaggttaa ggtctgctca
    17041 taatctttcc atagtccttc ttgcgtttga acagtttgat tatgttcttt ccaaacgcct
    17101 ccccactctt ccaactcagt attccatact tcttcgtaaa ttcctgcaac gggtagtccg
    17161 attgtaaaat ctttccgctc aacaggtacc atgttaaaga tacagactaa catttctccc
    17221 tttttaccct tacgaataaa ggaaagaaca ctctggtctc gattatccgc atcaatgact
    17281 tcaataccat catagctggt atcgatttcc cacagacagc gatgatcttt gtaaaactgg
    17341 tttagctgag aagtgaaata cttcatctta gcattcattg ggtcttctag gttagaccat
    17401 tccaactgtt cttcagattt ccattctagg aattgaccgt attcgctacc catgaagagc
    17461 aatttcttac cagggtgaca aatttggtac gtatagagat tgcgcaagcc tgcgaattga
    17521 ttgtaacgat ctccccacat cttatgcatc atactcttct tgccatgaac cacttcatcg
    17581 tgcgagaatg gcaagagata attctccttg aaaacataca taaagctgaa agtcaccagg
    17641 ttaaagtcat atttacgata aatcggatct tcttcgtaga aacggaggat atcattcatc
    17701 cagcccatgt tccatttgta gtcaaatcct agaccaccaa tctctttcat tcccgtaatc
    17761 ttggttgcag acgaactttc ttctgcaatc atcatcacat ctggatattc taacttaata
    17821 acttcattca agcgttgaag gaaataataa ccttcatagt tgagatttcc gccatcttta
    17881 ttaggtgtcc atggagcatc atcatagtcc aaatagagca tgttgctaac agcatccaca
    17941 cgaataccat ccaaatgata gatatcaatc caatgcttaa tgcaagaaat taagaaggac
    18001 tggacttcat tttttccaag gtcaaaatta agggcacccc aaccatggtt atgagcctta
    18061 ttatggtctt ggtattcaaa agtcggtgtc ccatcataat aggctaaggc atcatcattg
    18121 atggtaaagt gacctggtac ccagtccaca ataaccccaa tattatgggt atgacactcc
    18181 tcgacaaaat cttgaaactc ctctggtcta ccataggcat gctctaaagc gaagtaaccc
    18241 ataagctggt acccccaact caaacctagt gggtgggaca tcaagggcat aaactcaata
    18301 tgagtatagt tcatctcaac taggtaagga atgagttcat ctttcaattg ggcaaaacta
    18361 taaggactgc catcaggatt tctcttccaa gagccagcat gaacctcgta aatattgaca
    18421 ggacgttcag caaagcccca acgttttctt cgtgccagcc aaagtccatc cctccatttc
    18481 ttatcaggaa gctctgttac gattgcccct gttcctggac gagcctcata cctgacagca
    18541 aaagggtcaa tcttcatcag ttgatgacca ttttgacgtg tgacatgata tttgtaaata
    18601 tgcccttctt gagccatatt ggtaaagact tcccagaccc caaagtcatt tcttaccatt
    18661 ggaatctgat tttcaatcca attggtaaaa tcaccaacca agtgaacagc ctgagcatta
    18721 ggtgcccaaa cacggaaggt atagccatac tctccattta gttcttccct atgtgctcct
    18781 agataatgtt ggagataaaa attttcaccc gtcataaagg tttttaatgc ttctctatta
    18841 tccatatact ccctttctcc tgtaagcgtt ttctatgttt ttattatact acctttttag
    18901 agaagattca agtaaattac tatacttctt taattatttt gaaaatctac aacaagttca
    18961 cttactcgtt caattgtaaa tcaatatttt ttcaaaaaat tgcgaaaaca cctttctttt
    19021 tctactatag tgaaatgaaa taaaacatgc gcaaatcgat taaggaattt aatctaattt
    19081 ctaacaatgt cttagaaatc aaagtgtact attttaactt caatgtacta t
//
LOCUS       DAVBCN010000038        18755 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVBCN010000038 DAVBCN010000000
VERSION     DAVBCN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18755
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.038"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            65..652
                     /locus_tag="VQ497_001214"
     CDS             65..652
                     /locus_tag="VQ497_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2517522.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCSDELTILQILYGASLIS
                     FLWMYGQRKQVIKVNMKTRIQWLGIGFVSLLIISLCFSLIHAQESTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTIIAWCLYV
                     SLGMFLGLVRYKLDLWGSMGLHLVWNLLVYSLLLF"
     gene            1052..1861
                     /locus_tag="VQ497_001215"
     CDS             1052..1861
                     /locus_tag="VQ497_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2517523.1"
                     /translation="MLLQKELIPMIEANLPNMAYAEKDIAKFFLKQQPLNNYSCKALC
                     ECLNVSKATLTRFAKKCGFKGFRQFIFKYQEMIREKEKLALYTEATEKVLSDYEEMLR
                     KTYTVLDEVQLERIAEMIETAERVYLYGKVSSVLALQEMKMRFMRLGVIGEVLSDEDM
                     ILWNSLLLNENCLVIGASVSGQTDIVLEGLQKAADKGAKTVLMTTRKFDEEDCFFDEL
                     LLLASTNHLSYGNRISPQFPILLITDCLFSHYLESPERQYYYNQTIIHKEE"
     gene            1866..2567
                     /locus_tag="VQ497_001216"
     CDS             1866..2567
                     /locus_tag="VQ497_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2517524.1"
                     /translation="MSQIWTKEKFISQVHGGVIVSCQALPGEPLYNEEFSLMPFMAKA
                     ALEAGAVGIRANSVRDIKAIQKVVDLPIIGIIKRDYPPQEPYITATMKEVDELVECGT
                     TVIAFDATLRPRYDGLVINEFIKKIKEKYPNQLLMADVSNFDEGLYAFKSGVDFVGTT
                     LSGYTSTSVQSDEPDFELMKKLADFNIPVIAEGKIHYPEQLKKAYSLGVTSVVIGGAI
                     TRPKEIAQRFINVIK"
     gene            2592..3509
                     /locus_tag="VQ497_001217"
     CDS             2592..3509
                     /locus_tag="VQ497_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HET2517525.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQERVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHVACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAAPNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            3537..5078
                     /locus_tag="VQ497_001218"
     CDS             3537..5078
                     /locus_tag="VQ497_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HET2517526.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGNSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGAVAIVYSYTGGLKSVLWTDFIQGVILISGVVLALFVLMANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLATATVFYLIGTGLYVFYQVQNAGSAASNIPQDQIFMYFIAYQLPVGITGL
                     ILAAIYAASQSTISTGLNSVATSWTLDIQDVISKNMSDDRRTKIAQFVSLAVGLFSIG
                     VSIVMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTGNKVSAPKYTTIHDIPEIKADSS
                     WEVRH"
     gene            5097..5555
                     /locus_tag="VQ497_001219"
     CDS             5097..5555
                     /locus_tag="VQ497_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2517527.1"
                     /translation="MIISEQSDFRRYASINKHFSKVCDFLENTNLTDLVDGKVDIDGE
                     NVFANCMIYLADGVPGDIFETHKKYLDIHIVVENTEKMAVTSPVRAQSRVPFSEEKDI
                     AFYDSKDYQIVELLPGNMLVTFEEDLHQPKIHCNDEPVKKLVIKVLNEEK"
     gene            5557..7779
                     /locus_tag="VQ497_001220"
     CDS             5557..7779
                     /locus_tag="VQ497_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HET2517528.1"
                     /translation="MKKNIKQYVTLGTAVVLSAFVANSVAAQETETSEVSTPELVQPV
                     APTTPISEVQPKSGNSSEVTVQPRTVETTVKDPSSATEETPVLEKNNVTLTGGGENVT
                     NELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNHLVSRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPVPLEWPRDVGGRDLQISGGATYIDSVIVEKNNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLKKQGDTDYNYTIRENGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFKNNKKTEYRNGTHVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSTDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQIWIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN
                     "
     gene            7819..8928
                     /locus_tag="VQ497_001221"
     CDS             7819..8928
                     /locus_tag="VQ497_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2517529.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEYEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            9085..9975
                     /locus_tag="VQ497_001222"
     CDS             9085..9975
                     /locus_tag="VQ497_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2517530.1"
                     /translation="MKIIGIDIGGTTIKADLYNEFGTSLNQFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYILNHSIDGVGISTAGVVNANTGEIIYTGYTIPGYIGVNFTFEIEKRF
                     GLSTFVENDVNCAALGELWKGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLRAGDKVAKETFEIFVE
                     NLTKGLLTITYLLNPEILILGGGILAKKDILLPEIQSSLAKNAMDNRFLPKNLVAATL
                     GNEAGRIGAVKNFLDRISNK"
     gene            10000..10323
                     /locus_tag="VQ497_001223"
     CDS             10000..10323
                     /locus_tag="VQ497_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517531.1"
                     /translation="MTNSVFSTMQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKS
                     YDEKSHEELVSIESNLEMKQQNLIDEVNKTIKENYEKIQYISSSRRGEFVEKIIGRVV
                     EKYGY"
     gene            10313..12304
                     /locus_tag="VQ497_001224"
     CDS             10313..12304
                     /locus_tag="VQ497_001224"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HET2517532.1"
                     /translation="MAISQMKRISLLFSKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFPTIQISHTSNSNHGVIEGNDALIYLMNQQQYLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNRQINRVHELERHIQSNNEEIERLIKW
                     EKLEIIPAHLEQFFFCKGKVGTIPRTVDNRLYNSFLENNIEVQEIFSNDREYGVVVFY
                     QSSCSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENIIASLQDSKNNLID
                     LQWQIDYILSIYARQISKNNLLSTPHLVALEGWIEETRILYFIKVMDERFGHSIYIYE
                     SETLSDNQDEIPIKLTNHSLIEPFELLTEMYALPKYYEKDPTPVLAPFYFTFFGMMVA
                     DLGYGLLLFLGTMLALKIFHLPSETKRFLKFFNILGVAVAIWGGIYGSFFGYELPFHL
                     ISTTSDVMIILVVSVVFGFITVFAGLLASGLQKVRMKKYAEAYNSGFVWCVILLGLLF
                     IAVGMLMPDMRLLFVLGKWVSIFNAVGILVVSIIQAKSLSGIGAGLFNLYNISSYIGD
                     LVSFTRLMALGLSGASIASAFNLIVGLFPGTLAKLTIGLVLFILLHAINIFLSLLSGY
                     VHGARLIFVEFFGKFYEGGGKPFQPLKASEKYIKVITKN"
     gene            12320..12796
                     /locus_tag="VQ497_001225"
     CDS             12320..12796
                     /locus_tag="VQ497_001225"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HET2517533.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            12830..13411
                     /locus_tag="VQ497_001226"
     CDS             12830..13411
                     /locus_tag="VQ497_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517534.1"
                     /translation="MSNLENLRESVIEQAHEKGRMKLLDSKKKIDDEFEMQKSVIVKK
                     KEVEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALIEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNHLFSEQPISNESGLLI
                     SIGKIDDNYLYKTLIGSISKEESSSIANQIFIN"
     gene            13476..14483
                     /locus_tag="VQ497_001227"
     CDS             13476..14483
                     /locus_tag="VQ497_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HET2517535.1"
                     /translation="MDTNLFSKINTTISVKENDFITEDKFQKIIQSKDTETMAFILEA
                     TPYHLSIDVLENPSQTETSLMTKLVNDYRWAYAESPSNKIVTLFALRYVYHNIKVLLK
                     SKAAIKKDFSKLLIPIGIFDIESLKHLVSSLHSDTLPDFMVHEVESIWNEYETFNNIR
                     VLDVGADLAYFKHLKLLSNELDEVLSQVIVAMIDFYNIITVKRGLSQNKSHGDILQLL
                     SDEGSISAKEFIYIVENQEIFVWFNKINPSLDSIFSTYELKMQDATISSSELEFLCDL
                     LLYKILDQGRYNVDGPLVLARYLLGCEFEVKNLRMIISALQNTIPFGSIKERIRPHYG
                     S"
     gene            14473..14793
                     /locus_tag="VQ497_001228"
     CDS             14473..14793
                     /locus_tag="VQ497_001228"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HET2517536.1"
                     /translation="MEANKYKIGIIGSRDIILPFSMIGFDIFPAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HNIL"
     gene            14856..16631
                     /locus_tag="VQ497_001229"
     CDS             14856..16631
                     /locus_tag="VQ497_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HET2517537.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTIAIGQKVSTGDILGTVKETEVVNHKIMVPYGVSGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFSNVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGIPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGIYIDGKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIMEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIHLILETGGL"
     gene            16632..18017
                     /locus_tag="VQ497_001230"
     CDS             16632..18017
                     /locus_tag="VQ497_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HET2517538.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLSSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVRFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            18040..18651
                     /locus_tag="VQ497_001231"
     CDS             18040..18651
                     /locus_tag="VQ497_001231"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HET2517539.1"
                     /translation="MVRLNVKPTRMELNNLKERLKTAERGHKLLKDKRDELMRRFISL
                     IRENNRLRKEVESYLIDNLKSFAVAKSLKNSLMVEELFSIPSKEIELFIEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIDKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
BASE COUNT         6330 a         2653 c         3724 g         6048 t
ORIGIN      
        1 catgggtaga aaggattgtg caggtattag atttagatta tgaagattta tttcggaggt
       61 aattatgaaa agaataattc cagtttatat attccaacaa gtaaatgtcc tattggtatc
      121 tctatactta ttgaaatttc tttgtagcga tgagttaact atactacaga ttctctatgg
      181 agcgtcactc atttcttttt tatggatgta tggtcaacga aaacaagtga tcaaggttaa
      241 tatgaaaact aggatacaat ggcttggtat tggattcgtt agcctactga ttataagtct
      301 atgttttagt ctaatccatg cgcaagaaag cacgaatcaa gcaaacttaa ttggacttca
      361 acatcaaatt ccttggtttt catttttatt gtttttaatc aatgcgagta tggttgaaga
      421 atttctgtat agagaaattt tatggaactt ggttagaaaa ttagatattc gagttgcttt
      481 gacaagtgtt ttatttgcct tagcacatca tccaggaacc attatagctt ggtgtttgta
      541 tgtatcgctg gggatgtttt tagggcttgt acgctacaaa ttggacttat ggggcagtat
      601 ggggctacat ttggtgtgga acctactagt ttatagtttg ctgctttttt aaaataagtg
      661 atctgattta tctcataaat cagtagtttt tataggatta tttttaattc ggaaacaaaa
      721 atgtattaac agcataaaat aaagaaacta ggaacatttt ttttattttt tagaaataaa
      781 atgttgtata gaaattccga aagaaataat aaaatcaaaa taaaaattaa taaaataggt
      841 atatttgaac atattgacgt cgacaaatgt tcttgcaaga cataaaatac aaataaaaag
      901 aataaaaaat ttttatattt ttatatttgt tgttctgtta taaaatgaaa gaaattagaa
      961 actagtttct aaatcatgaa aaatattgaa aaaaaaaaaa ataagtgtta aaataaagaa
     1021 tgtaaacgtt tacttcaata aaggagctca tatgttactg caaaaagaac taattccaat
     1081 gatagaagct aacttaccaa atatggcata tgctgaaaaa gacattgcta aattcttctt
     1141 aaaacagcaa cctctgaata attattcatg taaggcattg tgcgaatgcc ttaatgtatc
     1201 caaagcaaca ttgactcgat ttgcgaaaaa atgtggtttt aaaggtttta gacaattcat
     1261 tttcaaatac caagagatga ttcgtgagaa agaaaagtta gcattgtata cagaggctac
     1321 agaaaaagtt ttatccgact atgaagaaat gttgagaaaa acttacacgg ttcttgatga
     1381 agttcaactt gagcgtattg ctgagatgat agaaactgct gagcgtgtat atctttatgg
     1441 taaagtaagt tctgttcttg ctttacaaga aatgaagatg agatttatgc gtctcggagt
     1501 cattggtgaa gtattatcag acgaggatat gattttgtgg aatagcttat tacttaatga
     1561 aaattgcctt gtcattggag catccgtttc aggtcaaact gatattgtac tagaaggtct
     1621 tcaaaaagct gcggataaag gcgctaaaac agttttaatg actacaagaa aatttgacga
     1681 agaagattgt ttctttgatg aactattgtt attagcttcg accaatcatc tctcgtatgg
     1741 caatcgcata tcacctcagt ttccaatact tttaattaca gactgcttat tctctcatta
     1801 tctggaaagt ccagagagac aatattatta caatcaaact attatccata aggaggaata
     1861 ataaaatgag tcaaatttgg actaaagaaa aatttataag tcaagtccat ggtggagtca
     1921 ttgtttcttg tcaagcttta cccggtgaac ccctttataa tgaagaattt agcttgatgc
     1981 cttttatggc taaagcagct ttagaggcag gagcagtggg cattcgcgca aattctgtgc
     2041 gtgatattaa agcaattcag aaagtagtag atttaccaat aattggaatt atcaaaagag
     2101 attatccacc tcaagaacca tatattactg ctacgatgaa agaagtagat gaacttgtag
     2161 aatgcggaac aacagtcatt gcatttgatg caactttaag accaagatat gatggcttag
     2221 ttatcaatga atttatcaaa aaaataaaag aaaaatatcc taatcaattg ttgatggcgg
     2281 atgtaagtaa ttttgatgaa ggtctctatg catttaaatc aggcgttgat tttgttggta
     2341 caacattatc agggtacaca agtacaagtg tacaatcaga tgagcctgat tttgaactaa
     2401 tgaaaaaatt ggctgatttt aatattccgg taattgccga aggaaaaatt cattatccag
     2461 aacaattaaa aaaagcttat agtttaggtg ttaccagtgt tgtcattggt ggagcgatta
     2521 cacgtccaaa agaaattgct cagcgattta ttaatgtcat caaataaaaa acaatataaa
     2581 ggagaaatct gatgaaagat ttaactaaat acaaaggcgt tattcctgca ttttatgctt
     2641 gctatgatga aaatggtgaa attagccaag aacgtgtaaa atctctggtg caatatttca
     2701 ttgacaaagg tgtaaaaggt atctatgtaa atggttcttc aggtgaatgt atttaccaaa
     2761 gtgtagaaga tcgtaaacaa attattgaag ctgttatgga agttgctaaa ggtaaattaa
     2821 cagttatcaa ccatgttgca tgtaataaca cgaaagatag tattgaattg gcaaaacatt
     2881 cagaaagtgt tggagtcgat gctattgctg ctatcccacc tatttacttc aaattgccag
     2941 aatattcaat tgcagcatat tggaatgcaa tgagtgaagc tgcgccaaat acagatttta
     3001 tcatttataa tattccacaa ttggcagggg ttgcgttgac tggtagtttg tatgcaacaa
     3061 tgcgtcaaaa tcctcgtgtg attggagtta aaaattcttc tatgcctgta caagatattc
     3121 aaatgtttgt agctgcaggt ggagaagatt acattgtatt caatggtcca gatgaacaat
     3181 atcttggtgg tcgcttgatg ggagcagaag ctggtattgg tggtacttat ggcgttatgc
     3241 cagatttgtt cttgaaattg gaaagtttga ttcaagaacg agatttagat actgctaaaa
     3301 aacttcaata tgctatcaat gaagttatct ataagatgat atcaggtaag gcaaatatgt
     3361 atgctgtagc aaaagaggtt ttgcgtctaa atgaaaaact tgatttaggt tctgttcgtc
     3421 aacccttaga agcattggca gaaggtgact tggaagttgc aaaacaagca gcagaactta
     3481 ttcaacaagc acgaaaagaa tttttataat aaataaaatc aatatggaga tatgttatgg
     3541 gcacaacagg atttacaata attgacttaa ttatcttgat tgtttattta cttgcggtgt
     3601 tggttgcagg tatctatttc tctaaaaaag agatgaaagg aaaagagttc ttcaaaggag
     3661 atggttcggt tccttggtat gttacttcgg tatccatttt tgccacaatg ctcagtccga
     3721 tttccttctt gggactcgct ggtaactctt atgcaggtag ctggatttta tggtttgctc
     3781 aattagggat ggtagtagct attccactga caattcgttt tatcttacct atctttgcac
     3841 ggatagacat cgatacggca tatgattact tggataaacg ttttaattct aaagcacttc
     3901 gtattatttc agcactcttg tttattattt atcaattggg acgtatgtct atcattatgt
     3961 acctcccatc agctggttta tcagtattga caggaattga catcaatatt ttgattattt
     4021 tgatgggtgc agttgcaatt gtttattctt acactggtgg tctaaaatcc gtattatgga
     4081 cagactttat tcaaggtgtg attctgatta gtggtgtcgt tttagcttta tttgtactga
     4141 tggctaatat taaaggtggc tttggtgcag tagcagaaac attagcaaac gggaaattcc
     4201 ttgctgcaaa tgaaaaactt ttcgatccta acttgctttc aaactccatc tttttaattg
     4261 tgatgggttc aggctttaca atcttgtctt cctatgcttc atctcaagat ttggttcaac
     4321 gttttactac aacacaaaat attaagaaac ttaataagat gttgttcaca aacggtgtct
     4381 tgtcacttgc aactgcaaca gtcttttact tgattggtac aggcttgtac gtattctatc
     4441 aagtacaaaa tgcaggtagt gcagctagca atatccctca agaccaaatc tttatgtact
     4501 ttattgcata ccagttacca gtaggtatca caggtttgat tttggcagcg atttatgcag
     4561 catctcaatc aactatttca acaggtttga actctgttgc aacttcatgg acattggata
     4621 ttcaagatgt catttctaaa aatatgtcag acgatcgtcg tacgaaaatt gcacaattcg
     4681 tatctctagc agtaggttta ttctcaattg gtgtttccat tgtcatggct cactcagata
     4741 ttaaatctgc atacgaatgg ttcaatagct tcatgggact tgtacttggt ctacttggtg
     4801 gtgtatttat tcttggattt gtttctaaaa aagcaaataa acaaggtgct tatgcagcgc
     4861 tgattgtatc aactatcgtc atggtattta ttaaatattt ccttcctcca acagctgtga
     4921 gctactgggc atattcattg atttcaatct ctgtatcagt agtttcaggt tatattgtat
     4981 ctgttcttac tggaaataaa gtatctgcac ctaaatatac aacgattcat gatattccag
     5041 aaattaaagc ggattcaagt tgggaagttc gtcactaata gaaaagagaa aataatatga
     5101 ttatttctga acaatctgat tttagacgat atgcttctat caataaacat ttttcaaaag
     5161 tctgtgattt tttagaaaat acaaatttga cagatttagt tgatggaaaa gttgatattg
     5221 acggggaaaa tgtttttgca aattgtatga tctatctagc tgatggagtc ccaggagata
     5281 tttttgaaac tcataagaaa tatttagata ttcatattgt tgttgaaaat actgaaaaaa
     5341 tggcagtaac ttccccagtt cgtgcccagt cacgtgtacc ttttagcgaa gaaaaggata
     5401 ttgcttttta cgatagcaaa gactatcaaa ttgttgaatt gcttccaggg aatatgttgg
     5461 tgacttttga agaagatctt caccaaccaa agattcattg caatgatgag cctgttaaaa
     5521 aacttgttat taaagtttta aacgaggaaa aataaaatga aaaaaaatat taaacaatac
     5581 gtaaccttag gtactgcagt ggtattatca gcatttgttg ctaactcagt tgcagctcag
     5641 gagactgaaa cttctgaagt atcaacacca gagttggtgc aacctgttgc accaacgact
     5701 ccgatttcgg aagtacaacc taaatcgggt aactcttcgg aagttactgt acaacctaga
     5761 acagtcgaaa ctactgttaa ggatccatct tctgcaacgg aagaaactcc tgtcttagaa
     5821 aaaaataatg ttactttaac agggggcgga gaaaatgtta ctaatgagtt aaaggataaa
     5881 tttactagcg gtgactttac tgtagtgatt aagtacaatc agtctagtga gaaaggctta
     5941 caagctctgt ttggaatatc taattctaaa cccggtcaac aaaatagtta tgtagatgtg
     6001 ttccttagag acaatggtga attgggaatg gaagcgcgtg atacttcttc caataaaaat
     6061 cacctagtat ccagacctgc ttcagtttgg ggtaagtaca aacaagaagc tgtgactaac
     6121 actgttgcag tagtagcaga ttcagtcaaa aaaacatatt ctttatatgc aaatggtaca
     6181 aaagtagtag aaaagaaagt ggataacttc ttaaacatca aggatattaa aggtattgat
     6241 tactatatgc ttgggggagt gaaacgtgca ggaaaaacgg cgtttggttt taacggaaca
     6301 ctagaaaata tcaaattctt taatagtgca ttggatgaag aaactgttaa aaagatgaca
     6361 acaaacgctg ttactggaca tttaatttat acggctaatg atacaacagg ttctaactat
     6421 ttccgtattc cagttctgta tacttttagc aatggtcggg tattttcaag cattgacgct
     6481 cgttacggcg gaactcatga ttttttgaat aaaattaata ttgctacaag ttatagtgat
     6541 gataatggta agacatggac taaaccaaaa ttaacattgg cattcgatga ttttgcgcca
     6601 gtaccattag aatggcctcg tgatgttggt ggacgtgact tacaaatcag cggtggtgca
     6661 acctatattg actctgttat tgttgaaaaa aataacaaac aagtactcat gtttgcggat
     6721 gtgatgcctg ctggagtaag ttttagagaa gctactagaa aagattcagg ttataaacaa
     6781 attgatggta attattacct taaattaaag aaacaaggtg atactgatta caattatact
     6841 attcgtgaga atggtactgt atacgacgat cgtaccaaca gaccaactga attttcagta
     6901 gataaaaatt tcggtattaa acaaaatggt aattatttga cagtagagca atattcggtt
     6961 tcatttaaaa ataataaaaa gactgaatat cgtaatggga ctcacgtcca tatgaatatc
     7021 ttctacaaag atgcattgtt caaagtagtg ccaacgaact atattgcata tatttctagc
     7081 aatgatcatg gcgaatcttg gtctgcacca actttattac ctcctataat gggacttaat
     7141 cggaatgcgc cttatttggg tcctggacgt ggaatcattg aaagctcaac tggacgtatt
     7201 cttattccgt cttacactgg taaagagtct gcattcattt atagtgacga taatggagca
     7261 tcttggaaag ttaaagttgt gccacttcct tctagttggt cagcagaagc acaatttgta
     7321 gaattgagtc caggagtaat tcaagcatac atgcgtacaa ataatggtaa aattgcatat
     7381 ttaacaagta cagacgcagg tactacttgg agtgcaccgg aatatttgaa atttgtttca
     7441 aatccaagtt atggaacaca attatcaatc atcaattata gtcaactgat tgatggtaaa
     7501 aaggctgtca ttttaagtac tccaaactcc acaaatggtc gtaaacacgg acaaatttgg
     7561 attggtctaa ttaatgatga taatacaatt gattggcgtt atcatcacga cgttgattat
     7621 agtaactatg gatactcata ttcaacattg acagagttac caaatcatga aattggattg
     7681 atgtttgaaa aatttgattc atggtctcgt aatgaacttc atatgaaaaa tgttgtacca
     7741 tatataacat ttaagattga agatctgaaa aagaattaaa gctgaaattt gaaaatatat
     7801 aaaaagagga taaaaattat ggtaaattac ggtattgttg gagctggata ttttggagct
     7861 gatttagctc gctcaatgaa caaaattgaa gatgcaaaag tggttgcggt atttgaccca
     7921 aatcatggag aagaagttgc tcaagagttg ggatcagatg tttgtgcaag tttagatgaa
     7981 cttgtagcac gtgaagatat tgattgtgtg atcgtagctt cacctagcta ccttcaccgt
     8041 gaaccagttg tgaaagctgc tcaacatggc aaacacgtat tttgtgaaaa gccaattgca
     8101 ttgtcttatg aagattgtaa agccatggtt gacgcatgta aagaaaataa tgttatcttt
     8161 atggctggtc acatcatgaa cttctttaac ggtgtacacc atgctaaaga attgattact
     8221 caaggtaaaa tcggtaaagt tctttattgc catgccgctc gtacaggttg ggaagaacaa
     8281 caaccaactg tatcatggaa gaaacttcgt tctcaatctg gaggacattt gtaccatcat
     8341 attcatgaat tagattgcat tcagtttatc atgggaggac ttcctgaaaa agcgacaatg
     8401 gtaggaggca atgtatatca taaaggtgaa aactttggtg atgaagatga tatgctcatt
     8461 gtaaacttag aatactctga tgatcgttat gctgttttgg aatatggtaa tgctttccgt
     8521 tggggtgaac actacgtctt gattcaagga actgaaggag ctatcaaact tgatttgttc
     8581 aatactggag gtactcttcg tgttaaaggt gaaggagaat cacacttctt agttcatgaa
     8641 actcaagagg aagatgatga ccgtacagct atctataccg gtcgtggtat ggatggagca
     8701 attgcgtacg gtaaaccagg agtacgttgc ccattatggt tgcaaacatg tattgataaa
     8761 gaaatggaat atctacatga catcattaaa ggtggagaaa ttacagaaga atatgaaaaa
     8821 cttctcaatg gtgtagctgc tttagaatca atcgctaccg ctgatgcatg tactttatca
     8881 gttaaagaag atcgaaaagt aagtctttca gaaatcacaa atgcttaact tttgtaaaac
     8941 agaatagtaa attcttatca ttatataatt tctaaagttc tgtgatacaa ctcattgaat
     9001 aaagaaatag agatgggact gggataatgc ccagtcccat tttttatcaa aaagtaatga
     9061 gatcaaaaat gtgggagtga tgaaatgaag attataggga tcgatattgg cggaacaaca
     9121 attaaagcag atttatacaa tgagtttgga acgagtttaa atcagttcaa agagatagaa
     9181 acaattattg actatgattt gggaacgaac cagatattaa atcaggtctg tgatttaatt
     9241 ggtgagtata ttttaaatca ttcaattgat ggtgttggga tttccactgc tggagttgtt
     9301 aatgctaata caggagagat catctataca ggctatacaa taccagggta tatcggagta
     9361 aactttactt tcgaaataga aaaacgtttt gggttgtcta cttttgttga aaatgatgtt
     9421 aattgtgctg cattaggtga attgtggaag ggacaagcca aagataagaa aaatgtagta
     9481 atggttacta ttggaacagg tataggaggc agtattattg tcaatggaca aattgttaac
     9541 ggatttaact atactgctgg tgaagtaggc tatattcctg taggtaattc ggattggcaa
     9601 agcaaagcct caacaaccgc attgattcat ttatatcaaa agaaaagctt gaaaactaat
     9661 caaactgggc gtactttctt tactgattta agagctggag ataaagtcgc taaagaaact
     9721 tttgaaattt ttgtagaaaa tctaacaaaa ggtttattaa cgattactta tctacttaat
     9781 ccagaaattc tcatattagg aggtgggatc ctggctaaaa aggatatttt gttacctgaa
     9841 attcaaagtt ctttagctaa aaatgcaatg gataataggt ttttgcctaa aaatcttgtg
     9901 gcagctacat taggaaatga agctggtcgt ataggagctg taaaaaattt cttagataga
     9961 atttctaata aatagtatga aagataagga ggtgtcacaa tgactaactc tgtattttcg
    10021 acaatgcaag atattgagaa tgttgcaacc gatattataa aatcatatga taatgagatt
    10081 tatacttata aagctgtttc ccaagaagaa ttggaaaaac tagaaaaaag ttatgatgaa
    10141 aaaagtcacg aagaattagt ttcaatagaa agcaatttag aaatgaaaca acagaacctt
    10201 attgatgagg ttaataaaac aatcaaggaa aattatgaaa agattcagta tatttcatca
    10261 agtaggagag gagaatttgt agaaaaaatt attggtaggg tggtagaaaa atatggctat
    10321 tagtcagatg aaaagaattt ctctactatt ttctaaaagt agtcttgatg atgttttaaa
    10381 aactattcaa gaactagagt cagtgcagtt ccgtgattta aaggttcagg ataactggtc
    10441 agaagctcta gaaaaagatg aggttgtatt tccaactatt caaatttctc atacttctaa
    10501 ttccaatcat ggggttattg aaggaaatga tgccttgatt tatttgatga atcaacaaca
    10561 atatttagaa gcaactgtag agaaattaca agaataccta ccgaaagaaa acacgtttaa
    10621 attattacag caacctccga taacgacctc ttatgaagaa ttagaaaaat ttggtaaagc
    10681 taatgttgct gagggtgttc ttaaaaaagt gaatcgtcaa ataaacagag ttcatgaatt
    10741 agaaagacac attcaaagta ataatgagga aatagagaga ttaataaaat gggaaaaatt
    10801 agaaattatt cctgcgcatt tagaacaatt ttttttctgt aaaggaaaag tcggaacaat
    10861 tccaaggact gtagataatc gcttatacaa tagtttttta gaaaacaata ttgaagttca
    10921 agaaatattt tctaatgata gagagtacgg tgttgttgtt ttctatcagt ccagttgctc
    10981 tattgatttt gatgaatact tatttgaacc atttgattat tctagaaagg aattaccgaa
    11041 gcagcgagta gtagatttgg atcaagaaaa catgcagtta ataactgaaa aagagaatat
    11101 tatcgcatcg ttgcaagatt caaagaataa tttgatagat ttacaatggc aaattgacta
    11161 tattttatct atctatgctc gtcaaatctc taagaataac cttttgagca ctccgcatct
    11221 agttgcacta gaaggatgga tagaagaaac tcgtatttta tattttataa aagttatgga
    11281 tgagcgtttt ggacattcta tttatattta tgaatcggaa acattgtcgg ataatcaaga
    11341 tgaaatacct atcaaattaa cgaatcattc tttaattgaa ccatttgaat tattgacaga
    11401 aatgtatgct ctgcccaaat attatgagaa agatcctaca cctgtattag caccatttta
    11461 ctttacattt tttggaatga tggttgctga tttaggctat ggtttactat tgtttttagg
    11521 aacaatgtta gcattaaaaa tttttcatct accttcagaa actaagagat ttttaaaatt
    11581 ctttaatata ttaggggtag ccgttgcaat ttggggtgga atctatggct cattttttgg
    11641 atatgagttg ccatttcatc tgatatctac aacctctgat gtcatgatta tattagtagt
    11701 gtcagttgtg tttgggttta ttacagtatt tgcaggtttg ttagcttcag gactacaaaa
    11761 agtaagaatg aagaaatatg cagaagcata taattcagga tttgtgtggt gtgttattct
    11821 gcttggcttg ttatttattg ctgtcggaat gttgatgcct gatatgagac tgttatttgt
    11881 attagggaaa tgggtatcta tttttaatgc tgtgggaatt ttggttgttt ctattattca
    11941 agccaaaagc ttgtcaggta ttggagcagg attgtttaat ctatataaca tttcatctta
    12001 tataggtgat ttagttagtt tcactcgatt gatggcatta ggattatctg gagcaagtat
    12061 agcatcagct ttcaatttaa ttgttggttt gtttccggga acattggcta aactaacaat
    12121 tggattagta ttattcattc ttttacatgc gatcaatatt tttctatcgt tactatcagg
    12181 atatgttcat ggagcacgtc tgatatttgt tgaatttttt ggtaagtttt atgagggtgg
    12241 aggaaaacca tttcaacctt tgaaggcttc tgagaaatat attaaggtta ttacaaagaa
    12301 ttaatggagg atatatataa tggaacattt agcaacttat ttttcaacct atggaggagc
    12361 tttcttcgct gcattgggaa ttgtattggc ggttggatta agtggtatgg ggtctgctta
    12421 tggagttggt aaggctgggc aatctgccgc agctttactg aaagaacagc cagaaaagtt
    12481 tgcctctgct ttgatattgc aattactgcc cggaacacaa ggattatatg gttttgttat
    12541 tggaattttg atttggttgc aattaactcc agaacttcct ttagaaaaag gcgttgctta
    12601 tttctttgta gctcttccaa ttgctattgt aggatacttt tcggctaagc atcaaggaaa
    12661 tgtagcagta gcgggaatgc aaatcttggc taaaagacca aaagaattca tgaagggagc
    12721 aattttggct gccatggtag aaacctatgc aattcttgcc tttgtcgtat cattcatttt
    12781 gacccttcgt gtataagaaa ttaatttgct attcaaagga ggtgtctaaa tgagcaattt
    12841 agaaaactta cgagagtctg ttattgaaca agctcatgaa aaagggcgta tgaaattatt
    12901 ggattccaaa aagaagattg atgatgaatt tgaaatgcaa aagtcggtca ttgtaaagaa
    12961 aaaagaagtg gaacatgaac gaaagttaaa agaattacaa cagaaatatc aaataatttt
    13021 tcaacaatta aaaaataagg aacgccaatc aacgttagta tcaaaacaga aaatattaaa
    13081 agaacttttt caatctgctt taatagaaat ggaatcttgg agtgcagata aagaaatgga
    13141 gttcatctat cgaattctgg aacgatattc acaacaagag gtcatagtaa cttttgggga
    13201 gcggacttta gctaaattca atctggaaca attagagaaa ttgaaattct cttttccaaa
    13261 tcatttattt agtgaacagc ctatctcaaa tgaatcaggc ttacttattt caataggtaa
    13321 aattgatgat aactatttgt ataaaacatt aattggatcg atttctaagg aagaaagttc
    13381 aagtatcgca aatcaaattt ttatcaatta aggatgaaat tggttaatcc ttcttagaaa
    13441 tttggagtat tccaataaaa ttagaaaggt attttatgga tactaatctt ttttcaaaaa
    13501 taaatacgac gatttcggta aaagaaaacg attttattac agaagataaa tttcaaaaaa
    13561 ttatacaatc caaagatacg gagacaatgg catttatctt agaagcaact ccctatcatt
    13621 tatcgattga cgttttagaa aatcctagtc agacagagac ttcgctaatg acaaaattag
    13681 tcaatgatta tagatgggcc tatgctgaaa gtccgtctaa taaaattgtg actttatttg
    13741 ctttacgata tgtttatcat aatatcaaag ttttattaaa atctaaggcg gcaattaaga
    13801 aagatttttc taaattatta attccaatag ggatttttga tatagaaagt ttaaaacatt
    13861 tagtttcttc cttacattca gatacacttc ctgattttat ggttcatgaa gtagaatcaa
    13921 tttggaatga gtatgaaact tttaataata ttcgtgtact tgatgtcgga gctgatctag
    13981 catattttaa acatctgaaa cttttatcta atgagttaga tgaggtgctg tctcaggtta
    14041 ttgtcgcaat gattgacttt tataatatta ttactgtaaa acgtggttta tctcaaaata
    14101 agagtcatgg ggatatttta caattacttt cagatgaagg aagtatttct gctaaagaat
    14161 ttatatacat tgtagaaaat caagaaatat ttgtgtggtt caataaaata aatccaagct
    14221 tagattcaat cttttcaact tatgaattga agatgcagga cgcaacaatt tcatcttctg
    14281 agttagaatt tttatgtgat ttattattgt ataaaatttt agatcaagga aggtacaatg
    14341 tagatgggcc gttagtactt gctagatatt tattgggatg tgagtttgaa gtaaagaatc
    14401 tcagaatgat tatatcagct cttcaaaata caattccctt tggatcaata aaagaaagga
    14461 tacgcccgca ttatggaagc taataagtat aaaattggca taattggtag ccgtgatatt
    14521 attttaccct ttagcatgat tgggtttgat atatttcctg cctatcaaga acaagaagct
    14581 ataaatactc taagaaaatt agcccaatct gattatggtg tcatttatat cactgaagac
    14641 attgcttcaa tgatattaga tacaattcgc cattatgatt ctcaagttgt gcctgctatt
    14701 attttattac cgactcataa acaaggttta aatttaggat taaaacgtat agaggataat
    14761 gtggagaaag cagtaggaca caatatttta taataatgta caaaattgtc tgtaatatta
    14821 ttctataatt tttggactta gtaaggagaa taactttgac tcaagggaag attataaaag
    14881 tatcgggacc tctagttatt gcatcaggta tgcaggaggc taatattcaa gatatttgcc
    14941 gtgtaggtaa gctagggtta atcggtgaaa ttattgaaat gagaagagat caggcatcta
    15001 tccaagtcta tgaggaaaca tctggtcttg gtccgggaga acctgttgtt acaactggag
    15061 aacctctctc ggttgaatta ggaccaggat tgatttctca aatgtttgat ggcatacaac
    15121 gcccattaga tcgatttaaa ttggctactc ataatgattt tctagttcgt ggggtagaag
    15181 ttccaagttt ggatagagat attaagtggc attttgattc cactatagca attggtcaaa
    15241 aagtgagtac gggtgatatt ctaggaactg tcaaggaaac cgaggtagtt aatcataaaa
    15301 ttatggttcc ttatggagta tctggcgaag tcgtttctat cgcatctggc gattttacaa
    15361 ttgatgaagt tgtatatgaa ataaaaaaat tggacggtag tttctataaa ggaacgctta
    15421 tgcaaaaatg gcctgttcgc aaggcgcgtc ctgtttctaa acgtttaatc ccagaagaac
    15481 cattaatcac aggtcaacga gttattgata cattctttcc agtaaccaaa gggggagcag
    15541 cagctgttcc tggaccgttt ggagcaggaa agacagttgt acaacaccaa gtagctaaat
    15601 tttccaatgt tgatattgtt atttatgtcg gttgtggaga acgtggaaat gaaatgacgg
    15661 atgtactgaa tgagtttcct gagttgattg accctaatac cggacaatca attatgcaac
    15721 ggacagttct gattgctaat acttcaaata tgcctgttgc tgctcgtgag gcttcaattt
    15781 atacaggaat taccatggct gagtattttc gtgatatggg ctactctgtc gccattatgg
    15841 ctgattcgac ttcacgttgg gcagaagcac tacgtgaaat gtcaggacgt ctagaagaaa
    15901 tgcctggtga tgagggttat cctgcttatc tgggaagtcg tatcgctgaa tattatgaaa
    15961 gagcgggacg ttctcaggtt ctagggattc cagaacgtga aggaactatt actgctattg
    16021 gagctgtatc gccacccggt ggagatattt cagaaccagt tactcaaaac actttacgga
    16081 ttgtgaaagt tttttggggg cttgatgctc cgttggcaca gagacgtcat tttcctgcaa
    16141 ttaactggct tacatcttat tcactctata aagacagtgt gggaatttat atagatggta
    16201 aagagaagac agattggaat agtaaaataa ctcgtgcgat gaactactta caacgggaat
    16261 ctagtttaga ggaaattgtt cgtcttgttg gaattgattc tctgtctgat aatgaacgac
    16321 taacgatgga aattgctaaa caaattcgag aagattattt gcaacagaat gcttttgatt
    16381 cagtagatac attcacttcg tttgcaaaac aagaagcaat gctaagtaat atcctaactt
    16441 ttgctgatca ggcaaatcat gctttagagt taggttctta ctttacagag attatggaag
    16501 gtaccgtggc agttcgagac cgtatggcgc gaagtaaata tgtttcagaa gatagattag
    16561 atgaaatcaa aattatttca aatgagatta cacatcaaat tcatttgata ttagaaacag
    16621 gaggtctata aatgagtgtt ataaaagaat acagaactgc tagtgaagtt gttgggcctc
    16681 ttatgattgt tgaacaagta aataatgtgt cttacaatga gttagttgaa attcaacttc
    16741 ataatggaga aattcgtcgt ggacaagttt tagagattca cgaagataaa gcaatggttc
    16801 aactttttga aggatctagt ggaataaatt tagaaaagtc taaaattcgt tttgctggtc
    16861 atgcactaga attggctgta tctgaggata tggttggtcg tatttttaat gggatgggaa
    16921 aaccaattga tggtggacca gatttaattc cagagaaata tttagatatt gatggtcaag
    16981 ctattaatcc tgtatctaga gattatccag atgaatttat tcagacaggg atctcctcta
    17041 ttgatcattt gaatactctt gtacgtggtc aaaaattacc agtattttca ggttcgggct
    17101 tacctcataa tgaattagca gctcagatag caagacaagc gactgtttta aattctgatg
    17161 aaaattttgc ggttgtattt gcagcaatgg gtattacttt tgaagaagct gagtttttta
    17221 tggaagaact cagaaaaaca ggagcgatcg atcgttcagt cttatttatg aacttggcaa
    17281 atgatcctgc aattgagcgt attgcaactc cccgcattgc tttaaccgcg gcagagtatc
    17341 tagcttttga aaaagatatg cacgttctag ttatcatgac ggatatgact aactattgtg
    17401 aagcgttacg tgaagtctcg gcagcgcgcc gtgaagttcc aggtagacga ggctatccgg
    17461 gatatttata tacaaattta tcaactctat atgaaagggc tggtcgctta gttggtaaaa
    17521 aaggttcagt gacacagatt cctattttaa caatgccaga agatgacata acacatccaa
    17581 ttcctgattt aactggatac attactgaag ggcaaattat tttgtctcat gagttgtata
    17641 atcaaggtta tcgtccacca atcaatgttt tatcttctct ctctcgatta aaagataagg
    17701 gatctggaga aggtaaaact cgtggagatc atgctccaac tatgaatcaa ctgtttgcag
    17761 cctatgccca agggaaaaag gttgaagagt tagcagtagt attaggagaa tcggctttat
    17821 ctgatgtaga taaattgtat gtgagattta caaagcgttt tgaagaagag tacatcaacc
    17881 aaggatttta taaaaatcga aatatagaag atacgttgaa tcttgggtgg gaattactat
    17941 caattcttcc tagaacagag ttaaaacgta tcaaagatga tttgcttgat aaatatttac
    18001 ctttggtaga agtttaatcc agaaatggag tgattatcta tggtacgttt gaatgtaaaa
    18061 ccaactcgta tggaattgaa taacttaaag gaacgtttga aaacagctga acgtggacat
    18121 aagttattaa aggataaaag agatgaattg atgaggcgat ttatttcttt gattcgtgag
    18181 aataatcgac ttcggaaaga agtagaaagt tatctaattg ataatctaaa atcttttgca
    18241 gttgctaaat cattaaagaa ctctctaatg gtggaggaat tattttcaat tccatcgaaa
    18301 gaaattgaat tatttattga gaaagaaaat atcatgagtg taacagttcc tagaatgcat
    18361 atgaatatta cttctcaaaa tgagaacagt gaatacagct atttatcttc taatagtgaa
    18421 atggatgatg tatttgctac aatgaatagt ttaattgata aattactaag actggcagaa
    18481 gttgaaaaaa cgtgtcagtt aatggctgat gaaatagaaa aaacacgtag acgtgtaaat
    18541 ggtttagaat attcgattat tccaaacttg tcggaaacta ttcattatat agaattgaaa
    18601 ctagaggagg cagaaagagc caatttagtt cgtattatga aagtgaagta gttcctttat
    18661 ttagattttt aattagatga acaaatatca gcttggataa ggcggtaagc ctttctaagc
    18721 ttttttaggc tctttgtcaa ctgtagtggg ttgaa
//
LOCUS       DAVBCN010000039        18268 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVBCN010000039 DAVBCN010000000
VERSION     DAVBCN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18268
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.039"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     regulatory      91..179
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            394..1062
                     /locus_tag="VQ497_001232"
     CDS             394..1062
                     /locus_tag="VQ497_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HET2517540.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVITEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVDKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     gene            1305..2087
                     /gene="thiM"
                     /locus_tag="VQ497_001233"
     CDS             1305..2087
                     /gene="thiM"
                     /locus_tag="VQ497_001233"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET2517541.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVMIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            2089..2718
                     /locus_tag="VQ497_001234"
     CDS             2089..2718
                     /locus_tag="VQ497_001234"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET2517542.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFGTQSKDDAGGTIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     regulatory      2927..3015
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3277..3837
                     /locus_tag="VQ497_001235"
     CDS             3277..3837
                     /locus_tag="VQ497_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2517543.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGFVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     gene            3838..5223
                     /locus_tag="VQ497_001236"
     CDS             3838..5223
                     /locus_tag="VQ497_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2517544.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIVERVQTVGILLQNPNHQFTMESLFEELVFTMENI
                     GYHLQEIDSKIAEVVQQCRCKDILHRLIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELVYDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGSITWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            5225..5875
                     /locus_tag="VQ497_001237"
     CDS             5225..5875
                     /locus_tag="VQ497_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HET2517545.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAIFPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQKLHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAYGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            5886..6578
                     /gene="tenA"
                     /locus_tag="VQ497_001238"
     CDS             5886..6578
                     /gene="tenA"
                     /locus_tag="VQ497_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HET2517546.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEINDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            6583..7107
                     /gene="thiW"
                     /locus_tag="VQ497_001239"
     CDS             6583..7107
                     /gene="thiW"
                     /locus_tag="VQ497_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HET2517547.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFAERID"
     gene            7107..7910
                     /locus_tag="VQ497_001240"
     CDS             7107..7910
                     /locus_tag="VQ497_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET2517548.1"
                     /translation="MQALTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSENQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            7903..8535
                     /locus_tag="VQ497_001241"
     CDS             7903..8535
                     /locus_tag="VQ497_001241"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET2517549.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(8744..9535)
                     /gene="thiD"
                     /locus_tag="VQ497_001242"
     CDS             complement(8744..9535)
                     /gene="thiD"
                     /locus_tag="VQ497_001242"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET2517550.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEGMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     regulatory      complement(9619..9717)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9901..10296
                     /locus_tag="VQ497_001243"
     CDS             9901..10296
                     /locus_tag="VQ497_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HET2517551.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTIQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            10307..10678
                     /locus_tag="VQ497_001244"
     CDS             10307..10678
                     /locus_tag="VQ497_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET2517552.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QVGEFGFACGMNMMHGKMIVE"
     gene            10688..12931
                     /locus_tag="VQ497_001245"
     CDS             10688..12931
                     /locus_tag="VQ497_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET2517553.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYIVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQSLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            13138..14913
                     /gene="spxB"
                     /locus_tag="VQ497_001246"
     CDS             13138..14913
                     /gene="spxB"
                     /locus_tag="VQ497_001246"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HET2517554.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            15024..15371
                     /locus_tag="VQ497_001247"
     CDS             15024..15371
                     /locus_tag="VQ497_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2517555.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(15488..15708)
                     /locus_tag="VQ497_001248"
                     /pseudo
     CDS             complement(15488..15708)
                     /locus_tag="VQ497_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            15879..16160
                     /locus_tag="VQ497_001249"
     CDS             15879..16160
                     /locus_tag="VQ497_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET2517556.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDSVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            16292..17236
                     /gene="manA"
                     /locus_tag="VQ497_001250"
     CDS             16292..17236
                     /gene="manA"
                     /locus_tag="VQ497_001250"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HET2517557.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTILVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVESWTLEGQDLELIVSHP"
     gene            complement(17276..18193)
                     /locus_tag="VQ497_001251"
                     /pseudo
     CDS             complement(17276..18193)
                     /locus_tag="VQ497_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822901.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
BASE COUNT         5449 a         3323 c         4053 g         5443 t
ORIGIN      
        1 gtttcaatat attatatata aagatatttt tcggtcaaat atcttgctag ggctttcatt
       61 ttttgctata ctggttcagt aattaaataa tagagacatt tggggtgctt taggctgaga
      121 taatacccat tgaacctgat acagttaaga ctggcgaagg gaaatgtgaa acgttttttc
      181 gtatggcgaa atgaacgatc taatcttgta agtcaactct aattcttgat tgtaattgga
      241 gttttttgtt tatataaact ggataggctt gtttagttag ctctctgcaa atgctctcga
      301 tgtgctttaa aaggatcaag tggaattacg attttgcttg atatttcttg gtgttttatt
      361 aattactaac aagtaaagag aggagaagta aatatggaaa cacaagacta tgcatttcag
      421 ccaggtttga ctgttggaga attattaaaa agcagtcaga aggattggca ggctgcaatc
      481 aatcatcgtt ttgttaagga actttttgcg gggacaattg agaataaggt cttaaaggac
      541 tatctgattc aagattatca cttctttgat gccttcttat ccatgctggg tgcctgcgta
      601 gctcacgcag ataaacttga atccaagctt cgttttgcca agcaactagg ctttcttgaa
      661 gcagatgaag atggttattt ccaaaaggct tttaaagagt taaaagtagc agagaatgac
      721 tatctagaag tgactttgca ccctgtaaca aaagcctttc aggatctaat gtattcggcg
      781 gtggcttcat cagactatgc ccatcttttg gtcatgctgg tcattacaga aggtctctat
      841 ttagactggg gttctaaaga tttggcttta cctgaagtct atattcattc ggaatggatc
      901 aatctccaca gaggtccttt ctttgcagag tgggttcaat ttctggttga cgaactcaat
      961 cgtgtcgata aaaatcgaga agatttgaca gaacttcagc aacgctggaa tcaagcagtc
     1021 gctttagaat tagctttttt tgatattggt tacgatgtct agagaaggct catgatattt
     1081 acaaatttga tgaggtaaga ctcttcgaaa atctcttcaa accacgtcag ttctatccac
     1141 aacctcaaaa cagtgttttg agcaacctgc ggttagcctc ctagtttgct ctttgatttt
     1201 cattgagtat aaatcattca atgtaaatga aaaaaattct taacgaaaga tagaaataaa
     1261 tcgaaatcag tagatcgtat aaagtgaaaa ggaaggattt caatatgaca agtttaaaat
     1321 tattaaaaga aaaagcacca ttggtcattt gtataaccaa tgatgtagta aaaaatttca
     1381 cagcaaatgg attagtagca ctgggcgcct caccagccat gagtgagttt ccagcagatt
     1441 tagaggattt gttaaagtat gctggtggtt tattaataaa cataggaaca ttgacagatg
     1501 aaaattggaa attataccaa gctgctctga aaattgcaga gaaatataat gtcccagcag
     1561 ttttagatcc tgtagcctgt ggagcaggag aatatagaaa aaaagtagca gatgatctaa
     1621 tcaacaatta taaactagca gcgattagag gaaatgctgg cgagattgcc tctttagtag
     1681 gaatagatgt ggcatctaaa ggagtagata gtgcgggcgt agataatatt gacgaaattg
     1741 ctctagcagc aaatgagaag ttcaatattc caatagtagt aacaggtgaa gtggatgcca
     1801 ttgctgttaa tggagaagtg gtaatgattc ataatggtag tgctatgatg ccgaaagtca
     1861 ttgggacagg atgcttatta ggagctgtag tagcaagctt tatcggacta gaaaaaggtc
     1921 aagaattgaa atcattagaa acagcaatgt tggtttacaa tatcgctgga gaaatggcag
     1981 aaaaacgtcc aaatggacat cttcctggga catttaaagt tgaatttata aatgccttat
     2041 acgagattac agatgaagat gtaaaggaat tcaaaagagt gaagtaagat gtttcataaa
     2101 gaattactaa aactatattt tatttgtgga acgactactt gccaaggaaa aaatctatat
     2161 acagtcgttg aggaagcctt aaaaggtggt ataaccttat ttcaatttcg agaaaaaggt
     2221 gagggtgctt tagaaggctt agaaaaactt gagctagcta tccaaattaa agaactttgt
     2281 aaaaaataca atgttccatt tatcgttaat gacgatatag atttagcaat ggaaattgac
     2341 gccgatggcg tacatgtagg tcaagatgat attggtgttg atgaaattag aaaattgatg
     2401 ccagataaaa taattggtct ttctataaga aacgaagaag aatttcaaca atcaaaagtt
     2461 gaatatgtag attatgtagg tgttgggcct gtatttggta cccagtcaaa ggatgatgct
     2521 ggtggtacta taggttatga aggtcttgaa ttgatgagaa aactattgcc acaaatgcct
     2581 ttagttgcaa ttggtgggat acagacgaaa catattaaag acattataaa gaccaatatg
     2641 gatggcgttt caatcatttc ggcaatatcg tatgcaaaaa atatagaaaa aactgttcgg
     2701 gaaatgagtg aacaatagat atctatcctt ggattctaag taagtaggat tttccttttt
     2761 taaaaagcta tgtccagatt cagcttgaat ggctaattca tcaaacaggt catttgtttt
     2821 ttgcaaaaga aagatctgct gtctttgtga gtgcaaggct gtatttccag ttcaatttct
     2881 tgaactagta taaaatgtgt gttataataa tcacataatt aaataataaa gacatttggg
     2941 gtgctttagg ctgagatgat acccattgaa cctgatacag ttaagactgg cgaagggaaa
     3001 tgtgaaatgt ttttttcgta tgtcgaaaag aactgtctaa tcttgtaagc tgaactctaa
     3061 tttgtatctg taattagagt tttttctttt acgaaactgg atgaagatga tatagtagat
     3121 tgaaataaga tgcgaacaaa tcaattagaa aattcaaatt aatttctaga aatattttag
     3181 tgttcttgtg tactgttcta aattcagtct gctatacaaa gtatttaggt cggtcttctt
     3241 gagtgcccca cttttcttta aaaaaaggag ttttttatgt tgaaaaaatg gcagttaaaa
     3301 gatgttatct tgcttgcttt cttgtctatc ttttttggtg gggttttcgt tggttcagga
     3361 tatgtgtata atattctcag tctactctta acacctcttg gtttgcaggc ctttgccaat
     3421 gaaatcctct tcggtctctg gtgtatggct gcgcccattg ctgccatctt tgttccgaga
     3481 gtcggaagtg caacgattgg agaagtgcta gctgcgcttg ctgaagtcct ttatggtagc
     3541 caatttggtc taggagctct tttgtctggc tttgttcaag gtttgggaag tgaatttggt
     3601 tttatcgtaa ctaagaatcg ctatgaaagt tggctctctc taactgctaa tagtattggg
     3661 attacgcttg ttagctttgt ctatgaatac attaagttag gttactacgc cttttccctt
     3721 ccgtttgtcc tttccttgct tgtggtacgt tttatttctg tttatttctt ctgtaccatc
     3781 ttggttcgtg ccattgtcaa actctatcat cagtttgcaa ctggaggaaa agcatagatg
     3841 ggtctggaac tacgagcgat tcagtcccca atcttctctg agccgtttga ttttactttt
     3901 catgcgcaag cctttacctt gttagttggg agtagtgggt ctggaaaatc cagcctcttt
     3961 caaatgattg cccaagttag ttctcttccc tatagcggtc aagtcctgat agatgggagc
     4021 gaggtcagtc agctttctat cgtcgaacgt gtccagacgg ttggtattct cttgcaaaat
     4081 cctaatcatc aatttaccat ggagagcttg tttgaggagt tggtttttac catggaaaat
     4141 atcggctatc accttcagga aattgattct aaaatagcag aggttgtcca gcaatgtcgt
     4201 tgcaaggaca tcttgcaccg tctcatccat cacttatcag gtggggaaaa gcaaaaagca
     4261 gcgctggctg tcctctttgc catgaatcct agggtctatc tcttggatga gcccttcgct
     4321 tccattgacc gcaagagcag aatcgagata ttggagattc taaaagagtt ggtctatgat
     4381 gggaagacag ttattttgtg cgaccatgat ttatctgact ataaagccta tatcgaccat
     4441 atggtggagc taagagacgg aaaactaagg gaagtgtttc aaatcccttc ctatgagatg
     4501 acacaggttg cttcaaagga agttgcttct agcccggaac tattccatat gaaccgtgtg
     4561 actggtgagc ttggtaatcg ccccctcttt tcaattgctg atttcacatt ctatcaaggg
     4621 atttcctgta tcctgggtga caatggtgtc gggaaatcaa ccctctttcg gtctattctt
     4681 caatttcaaa agtataaggg gagcattact tggaagggtt cggtcctgaa aaagaaaaag
     4741 agtttgtatc gtgatctgac tggtgttgtt caggaagctg agaagcagtt tatccgagtc
     4801 agtctgcgag aggagcttca attagatgga cctgattctg aaagaaatca gcggattttt
     4861 caagctttac gatattttga tttggagcag gcagtcgata agagtcccta tcaattaagt
     4921 ggtggtcagc aaaaaattct tcagctcctg accatcttga ccagtaaggc ttccgtgatc
     4981 ttgctagatg aaccttttgc aggtttggat gatagagcct gccattattt ttgcaagtgg
     5041 attgtggagg agaggaatca aggaagaagt tttctgctca ttagtcatcg tttagaccct
     5101 ttgatttctg tggttgatta ttggattgag atgactagtc agggtcttcg gcatgtgaaa
     5161 gaagtgacca ttaccaaacc acttacatct cagagtagca atacccaagg ggaggtgaga
     5221 tagtatggtc aaagtagcaa cccagacacc gattatcagt ctcttcttgc tgattttatc
     5281 cttggaaaca tctttcattc cttcgattgc tctgaatctt tcggtagtcg cattttgtat
     5341 tctctttatg ctctattacc gtcgatttaa aatgttagct tggatgatca tacttgccat
     5401 tttcccatct tttgccaact actgggcagt tcagttacac ggagatgctt cacaggcagt
     5461 catgcttgga acgagggcct ttgtgacagt ttgtatcggc cttgtctttg tttcctctat
     5521 ttcactaaaa gagcttctct tgtacttggc tcaaaagggg ctatcacgct cttggtccta
     5581 tgccttgatt gtggtattca attcttttcc tctcattcag caagaaatca agtccctcaa
     5641 agaagcttgc ctattacgtg gtcaaaaact acatttttgg tcgcccttga tttacagtaa
     5701 ggttctgatg acagtcttta ggtggcgcca tctttacctg agagctctat ctgcttacgg
     5761 atatgacgaa catgcacagt tgaagaatag ctatcggact ttttatattc ctaaaaaaac
     5821 aaaattaatc tacctgcttt tctttttatt gcttcaaacc agtctatttt tataaaggag
     5881 ttattatgga atttacagat attgcgatgg aattatccaa gaaagcttgg caggcttcct
     5941 ttcatcatcc ctttatttta caattacaag aggggaattt agaacctgcc attttccgct
     6001 attacctgat tcaggatgcc tactatctga aggctttctc agaaatctat catcttttgg
     6061 ctgataagac ttcaaaccaa gagatgaaaa gactcttgaa acaaaatgct cagggtttag
     6121 tggagggtga gttatttatt cgccaacaat ttttcaagga attggaaatc aacgaccagg
     6181 aaatggagca acatccaatc gctccaacct gttatcatta catttctcat atttataggc
     6241 aatttgcaga accgaactta gccatagctt ttgcaagctt gcttccttgt ccttggttat
     6301 accatgatat aggcaaatca cttaatctta aaccatcacc aaatcctctc tatcaacaat
     6361 ggattgaaac ttatattacg gatgagttag agcaacagat cagagaggag ggagcactgg
     6421 tcaatcagct ttatcgagaa agtgatgaga cagataagca aaaaatgcta gatgccttcc
     6481 acatcagtgt tcatatggaa gccaagtttt gggagatggc ttaccaacac cagacatgga
     6541 agagcgattt acagtcttta gaaaaaggag aagaatagaa acatgagaaa gcaccaacta
     6601 caagttcaca aattgaccat tttatctatg atgattgccc ttgatgtagt ccttacacct
     6661 atctttcgga ttgagggaat ggcaccgatg tccagtgtag tcaatattct agcgggaatc
     6721 atgatgggac ctgtttatgc cttggctatg gctacagtga cagcctttat ccgtatgacg
     6781 actcaaggga ttccgccttt agctctcaca ggagcgactt ttggagccct tctagcaggt
     6841 ctcttttata agtacggtcg aaaattttac ttttctgcct tgggagaaat tgtgggaaca
     6901 ggtattattg gttctattgt ttcctatcct gttatggtgc tctttacagg atcggctgct
     6961 aagcttagtt ggtttatcta cactcctcga tttttcggag caaccttgat tggtacagcg
     7021 atttccttta ttgcatttag atttttaatc aagcaggaat tctttaaaaa agtgcaagga
     7081 tatttctttg ctgaaaggat agactgatgc aggcattgac aaatcctttt cctataggct
     7141 ctagttccct cattcactgt atgaccaatg agatttcttg tgagatgctg gcaaatggga
     7201 ttttggctct gggatgcaaa cctgtcatgg cagatgatcc ccgtgaagtt cttgatttta
     7261 ctaagcaaag tcaggctctc ttcatcaatt tggggcattt gtcagctgag aaggaaaaag
     7321 caatccgcat ggcagcttcg tatgcaaacc aatcttctct cccgatggta gtagatgcgg
     7381 ttggcgtaac gacttcatcc attcgtaaga gcttagttaa agacctttta gactatagac
     7441 ctacggtcct taaaggaaac atgtcagaaa ttcgaagtct tgttggatta aagcaccacg
     7501 gcgttggggt cgatgcgagt gctaaagatc aagaaacgga ggatttgctt caagtcttga
     7561 aagactggtg tcagacctat cctggtatgt ctttcttagt tactggcccc aaggacctta
     7621 ttgtttcgga gaatcaagtt gctgtactgg agaatggctg tactgaatta gactggataa
     7681 cagggacagg agacttggtt ggagccttaa cagccgtttt tctcagccaa ggaaagactg
     7741 gttttgaagc ttcttgctta gcagtctctt atctcaatat cgctgctgag aaaatagttg
     7801 ttcaaggaat gggattggaa gaatttcgtt accaagtact caatcagctt tcgctcctaa
     7861 gaagagatga aaattggcta gataccatca aaggagaggt ttatgaatag agaagcactt
     7921 agactatatc tggtaaccaa tcgctatcaa gattccgtgg aaagctttct tgcaaaagtt
     7981 gagacggcct gccgttcagg ggttaccata gtccaattgc gagaaaaaaa tctcacaacc
     8041 aatcaatatt atcaactggc aaaacaagtc aaggaaataa cagatgccta tcaggtcccc
     8101 ttgataatcg atgatcggtt ggatgtttgt cttgcggttg atgcagcagg tctgcatatc
     8161 ggagatgatg aattaccagt ttcggtagct cgcaaagtct tgggtcctga aaaaatcctc
     8221 ggtgtcaccg ctaaaacggt taaaagagct ctggaagcgg agaaatcagg tgcagattac
     8281 ttgggtacag gagccatttt cccaaccacc accaaggaaa atgcgcccat tactctgata
     8341 tcgaccttga aaacaatttg ccaaacggtt gccattccag tagttgctat tggaggcttg
     8401 acttcggaga atattgatca gcttatgggc actggcatag ctggtgtagc tgttgtacgt
     8461 gatttgatgc aagcagaaga tattgaggca aaaacgcaag cctttttaac aaagttgcat
     8521 gacattcttt cctaattaat actcaatgaa aatcaaagag taaactagaa agctagtcgc
     8581 aggttgctca aagtactgcc ttgaggtttt agataagact gacgaagtca gctcaaaaca
     8641 gtgttttgag gttgcagatg gaagctgacg taatttgaag agattttcga agagtataaa
     8701 aactctctaa ttcccttaaa gtgggaacta gagagttttt ttcttaatct ttaaaagttg
     8761 tatggttgac tggaccagaa ccatgaccga gttgaggggc atcttgaata gcttttgtga
     8821 taaaggcctt ggccttatca actgcctggt aaagactctt gcccttggct agttcagcag
     8881 taatcactgc agcaaaggta catccagtac catgggtgtg acaggtttga attcgtgggc
     8941 tttcccagac aaattgttca ttcttggtaa agaggaaatc tttagcacca cctttgagat
     9001 gtccgccttt gataaccaca gactgaggac caaattcttt taaaatcagg cgaccagcac
     9061 gctgcatgcc ttcggggtca tggattgaaa aaccaacaat ctcttctgct tcaggaagat
     9121 ttggcgtaat aatagttgct agaggtagta agtttgtttt gagatagtct ctagcacttg
     9181 agtcaatcaa ggcatctcca cttgtagcaa ccataacagg atcaaggaca tagggacaat
     9241 ccagtttttt aagatagggt tggatgattt ccatgatttc agtagtagcc aacattccag
     9301 tttttacagc ctgaggtgga atatcagaaa agacactctc caattgggct ttcaacattt
     9361 gaggagaaac gtgctcgatt agctgaacac ctctggtatt ttgagcgaca agactggtta
     9421 caacagccat tccatagaca tctctcgctt ggaatgactt taaatctgcc ataatgccag
     9481 caccaccact agggtcagtc cctgcaatgg tcaaagcaac gggtaaataa gtcatctaaa
     9541 acctcccaac caactgaagt ttgtattctt aagaacaagc tagtctgttt tagaaaggca
     9601 atagaagact gctagtcccc attatcattt ccacttccat cagctagcat tacctagatt
     9661 gagtcagagg gatctaacag tcgttaatct cagctttacg cacccctagt ggttgttttc
     9721 ttttttcttt agtatagcat atttttattg tttatatagt aatatttgac tgcaaatcgt
     9781 ttttcaaatg attaaaattc tatttttcga agaaaatatt atttctataa ttgacaaatg
     9841 tagattttaa gagtatactg atgagtgtaa ttgacaaatg tagattttgg aggtatggta
     9901 atgcagattt cagatgcaga atggcaggtc atgaagatta tttggatgca gggggagcag
     9961 accagtacag atttgattag ggttttggca gagcggttcg actggtccaa gtcgaccatt
    10021 caaactcttt tggctcgttt ggttgagaaa gagtgtctga ctcggaaaaa agaaggcaag
    10081 tcctttgttt attcagccct tttaactcta gaccaaagtc gggatttact tgtccaagat
    10141 atcaaagaca aggtttgttc ccgtaggatt aggaatttgt tggctgactt gattgtagaa
    10201 tgtgaattta ctcagactga cttggaagac ttggaagctg tgatttcaga gaagaaatca
    10261 agtgctgtaa cagaagtaag atgtaattgt atgtaaagga gacgtcatgt taaatagtat
    10321 tgtaaccatt atttgtattg cccttatcgc gtttatcttg ttttggtttt tcaaaaagcc
    10381 tgaaaaatct ggacaaaaag cccagcaaaa aaacggatac caagagattc gagtggaagt
    10441 catgggaggc tatactcctg agttgattgt cctcaagaaa tcagtgccag cacgcattgt
    10501 ctttgaccgc aaggatcctt caccatgtct ggatcaaatt gtttttccag attttggtgt
    10561 acatgcgaac ctgccaatgg gggaagagta tgtagtggaa atcacgcctg agcaggttgg
    10621 agagtttggc tttgcttgtg gtatgaacat gatgcacggc aagatgattg tagagtaggt
    10681 ggagactatg acagaaattg tgaaagcaag cttagaaaat ggcattcaaa aaatccgtat
    10741 ccgagctgaa aaaggctatc atccagccca tatccagctt caaaagggaa ttccagctga
    10801 gattaccttt catcgtgcta ctccttcaaa ctgttataag gaaattctgt ttgaagaaga
    10861 aggtatcttg gaaccaatcg gcgtagatga ggagaaagtc attcgtttta cacctcaaga
    10921 attagggcga catgaatttt cttgtggcat gaagatgcaa aagggaagct atatagtcgt
    10981 tgagaagact cgaaaatctc tatctctcct gcaacgtttt tggattacta gtatctttac
    11041 tgtgcctctt gtgattctca tgattgggat gttgacaggt agcattagtc atcaagtcat
    11101 gcattgggga acctttctag caacaacgcc tattatgtta gttgcaggta agccatatat
    11161 ccagagtgct tgggccagtt ttaaaaagca caatgccaac atggatacct tggttgcgct
    11221 gggaactcta gtggcttatt tctatagcct agttgctctc tttgctggtc tccctgttta
    11281 cttcgaaagt gctggattta tcctcttttt cgttcttttg ggagcagttt ttgaggaaaa
    11341 aatgagaaaa aatacgtccc aagctgtgga gaaattactg gacttgcaag ctaaaaccgc
    11401 agaagtcttg agtgatgata gttatgtcca agttcctttg gaacaagtca aggtaggcga
    11461 cctgattcga gtgcgtcccg gtgaaaagat tgctgttgat ggtgtcgtag tagaaggtgt
    11521 ctctagtatt gacgaatcca tggtgacagg tgagagtctg cctgtggaca agacagttgg
    11581 agatactgtc attggctcaa ccatcaatca tagtggaacg cttgtcttta gagcagaaaa
    11641 agttggctca gagactgttt tggctcagat tgtggatttt gtgaagaaag ctcagacaag
    11701 tcgtgcgccg attcaggact tgacggataa gatttcaggg atttttgtcc cagtagttgt
    11761 cattttagga atcatgacct tttgggtttg gttcgtcttg ctcagggata gtgtggtcgt
    11821 gcttggagct agctttgtgt cctctcttct ctacggagtg gcggttttga ttatcgcctg
    11881 tccttgtgcc ttgggacttg caacaccgac agcccttatg gtggggacag gacgtagtgc
    11941 caagatgggg gttctcctca aaaatggaac tgtcttacag gaaatccaga aagttcaaac
    12001 tcttgtcttt gataagaccg ggactttgac ggaagggaaa cctgtggtaa cagatatcat
    12061 cggcgacgaa gtagaagtgt ttggattggc agcctccttg gaagatgctt ctcaacaccc
    12121 actggctgag gctatcgtta agcgagcgag tgaagctgga cttgagtttc aaactgttga
    12181 aaattttcag tccttgcacg ggaaaggtgt ttcagggcga atcaatggaa aacaagtttt
    12241 acttggaaat gctaaaatgc tggatggtat ggatatttct aatacttatc aagataaact
    12301 agaagaacta gaaaaagaag ctaagacagt tgtgttctta gctgttgaca atgaaatcaa
    12361 aggcttgctt gctttgcaag atattcctaa ggaaaatgct aagctagcca tcagtcagct
    12421 aaaaaaacgt ggtctccgaa cagtcatgct gacaggagac aatgctggtg tggcgcgtgc
    12481 tattgcagat caaatcggaa ttgaagaggt cattgcaggc gtcttgccag aagaaaaagc
    12541 ccatgaaatc cataaactgc aacagtcagg gaaagtagcc tttgttgggg acggtatcaa
    12601 tgacgctcct gcccttagtg tagcagatgt ggggattgct atgggagctg gaacagatat
    12661 cgccatcgag tcagcagatt tggtgttgac aaccaataat cttttaggag tggttcgtgc
    12721 ctttgatatg agtaagaaaa cctttcatcg aattctactc aatcttttct gggcttttat
    12781 ctacaatgtt gtcggaattc cgattgcagc aggagtcttt tcaggtgttg gactggctct
    12841 caacccagaa ttggcaggtc tagccatggc ctttagttct gtatctgttc tgaccagttc
    12901 ccttctgtta aattttagta aaatagacta aaagtaatga ctagatttct ttgttataaa
    12961 acagaaatga cagaactttc aaaactgtat tagaatagag aatagagagt tttgagcaga
    13021 tttttagaaa agtcagcata aatatgatac agtggaatag taaaaatttg gagaacgttt
    13081 ccaattctat gtaatcgtat tctccaaatc taaaaaaatt gaaggagagt tatcattatg
    13141 actcaaggga aaattactgc atctgcagca atgcttaacg tattgaaaac atggggcgta
    13201 gatacaatct acggtatccc atcaggaaca ctcagctcat tgatggacgc tttggctgaa
    13261 gacaaagata tccgcttctt acaagttcgc cacgaagaga caggtgctct tgcagcggtt
    13321 atgcaagcta aattcggcgg ctcaatcggg gttgcagttg gttcaggtgg tccaggtgcg
    13381 actcacttga ttaacggtgt ttacgatgca gctatggata acactccatt cctagcgatc
    13441 cttggatcac gtccagttaa cgaattgaac atggatgctt tccaagagct taaccaaaac
    13501 ccaatgtaca acggtatcgc tgtttacaac aaacgtgtag cttacgctga gcaattgcca
    13561 aaagtaattg acgaagcctg ccgtgctgca atttctaaaa aaggtccagc tgttgttgaa
    13621 attccagtaa acttcggttt ccaagaaatc gacgaaaact catactacgg ttcaggttca
    13681 tacgaacgct cattcatcgc tcctgctttg aacgaagttg aaatcgacaa agctgttgaa
    13741 atcttgaaca atgctgaacg cccagttatc tatgctggat ttggtggtgt taaagctggt
    13801 gaagtgatta ctgaattgtc acgtaaaatc aaagcaccaa tcatcacaac tggtaaaaac
    13861 tttgaagctt tcgaatggaa ctatgaaggt ttgacaggtt ctgcttaccg tgttggttgg
    13921 aaaccagcca acgaagtggt ctttgaagca gacacagttc ttttccttgg ttcaaacttc
    13981 ccatttgctg aagtttacga agcattcaag aacactgaaa aattcatcca agtcgatatc
    14041 gacccttaca aacttggtaa acgtcatgcc cttgacgctt caatccttgg tgatgctggt
    14101 caagcagcta aagctatcct tgacaaagta aacccagttg aatcaactcc atggtggcgt
    14161 gcaaacgtta agaacaacca aaactggcgt gattacatga acaaactcga aggtaaaact
    14221 gagggtgaat tgcaattgta tcaagtttac aatgcaatca acaaacatgc tgatcaagac
    14281 gctatctact caatcgacgt aggtaacact actcaaacat ctactcgtca ccttcacatg
    14341 acacctaaga atatgtggcg tacatctccg ctctttgcga caatgggtat tgcccttcct
    14401 ggtggtatcg ctgctaagaa agacaatcca gatcgccaag tatggaacat catgggtgat
    14461 ggagcattca acatgtgcta cccagacgtt atcacaaacg ttcaatacga ccttccagtt
    14521 atcaaccttg tcttctcaaa tgctgagtac ggcttcatca agaacaaata cgaagataca
    14581 aacaaacact tgtttggtgt agacttcaca aacgctgact acgctaaaat tgcggaagct
    14641 caaggagctg ttggattcac agttgaccgt atcgaagaca tcgatgcagt tgttgcagaa
    14701 gctgttaaat tgaacaaaga aggtaaaact gttgtcatcg atgctcgcat cactcaacac
    14761 cgtccacttc cagtagaagt acttgaattg gatccaaaac ttcactcaga agaagctatc
    14821 aaagccttca aggaaaaata cgaagcagaa gaactcgtac cattccgtct cttcttggaa
    14881 gaagaaggat tgcaatcacg cgcaattaaa taattcctct cgccgaaaat caaatatgaa
    14941 acttgtagac tttattcttt gattttcata gagcagaact ttaaaagatc ggagcaattc
    15001 ggtcttttta tggaggagag taaatgaatt taaatcaatt agatattatc gtttcaaatg
    15061 ttccccaagt ctgtgctgac ttggagcata ttttggataa aaaggcagat tatgctgatg
    15121 atggttttgc tcagtttacg attggtagtc actgccttat gttgtcacaa aatcatttgg
    15181 ttcctttgga aaactttcag tcaggaatca ttattcatat cgaggttgag gatgtagacc
    15241 agaactacaa acggttgaat gagcttggta taaaggtttt acacggacca actgtaaccg
    15301 attggggaac agagtcctta ttagttcaag gacctgctgg tctagtgctt gatttttatc
    15361 gtatgaaata ggtcgcgtag tcatttgacg ttaaccttag ctatttttaa aatagaaatt
    15421 gagaatatga actattgaaa gatcggagca atccggtctt tttatataaa gaaattgcat
    15481 ataatactca tctgctttca aaaagcattc tattccatct ccgattaacg atggacttta
    15541 tcacctcctt ctccagtcct tgtatgacat cttccaaagt tcgaaaggct ttattcttaa
    15601 atccacgttt atgaatctct ttccacactt gttcgatggg ttcatctctg gtgtgtatgg
    15661 aggaataaat gcaaaaccaa tattagtcgg agtctttata agatttgccc atcagacgaa
    15721 atagaatgat ttgaaggcat ttatgatatt tagctgtacg agagtcttta aaaagtgttt
    15781 tgatggtttg gatttcttct ttagttgatt tcatatcact attatataat gctttttgat
    15841 tttagtatag tattatcgta gaaaatggac tcggtgctat ggatacaccg gatgagaatg
    15901 gttatgtagc agatgactat cggattactt acttagaggc ccacatcaag gccatgcgag
    15961 atgccattta caaagacggg gttgacttgc ttggttatac gacatggggc tgtatcgatt
    16021 cggtttcagc tggcacaggg gaaatgaata agcgctatgg ctttatctat gtagatcgag
    16081 ataatgtagg aaatgggact ctcaaacgta gcaagaagaa gtccttctat tggtacatgt
    16141 catttatagc aatggtgtaa gcatcgagta gagcacattt gtttactatt tttataaaat
    16201 cttctccctg ctttataaga tgtaaaaaag agttcaaatg gaactctttt tttgctataa
    16261 tgaggggaga aaaatcagac aggagattaa gatgtcagaa ccattatttt tacaatcagt
    16321 tatgcaagaa aaaatctggg gtggtaccaa gctacgtgat gagtttggct acgacatccc
    16381 aagtgaaaaa gtcggagagt actgggctat ctcagctcat ctaaatggtg tttctaaagt
    16441 tgccaatggt cgttacgagg gaacagatct tgctactttg tatgcggaac accgtgaatt
    16501 atttggcaat cgtccagaac ctgtatttcc acttttgacc aagatcctcg atgccaacga
    16561 ctggctcagt gtccaagttc acccagacga tgcttatgga ctcgagcatg aaggcgaact
    16621 tggaaaaaca gaatgttggt acatcattgc agcagatgaa ggttcagaga ttatctacgg
    16681 tcacaatgcc aagtcaaaag aagaactccg ccagcaaatc gaggataaga actgggatgc
    16741 gctgttgaca aaagtgccag ttaaagcagg agatttcttc tatgtaccaa gcggtaccat
    16801 gcacgctatc ggggcgggta tcttgattct tgaaacccag cagtctagcg ataccactta
    16861 ccgtgtctat gactttgacc gtaaggatga caagggcaat ttacgtgaac ttcaccttga
    16921 aaaatctatc gatgtcttga acattggtga acccgcaaat agccgtccag tgacaatcaa
    16981 agcagatgat ctacgttcca ctatccttgt atccaatgat ttcttcgcag tttacaaatg
    17041 ggaaattact ggaaaagtta actttgaaaa gacagctgac tacagcttgt tgagtgtctt
    17101 ggctggtcaa ggtcaactaa ctgttgacgg gaaaaactat ccaatccaaa aagggagaca
    17161 ctttatccta ccaagtgatg ttgaatcttg gactctagaa gggcaagatt tagaattaat
    17221 tgttagtcat ccataaaaaa gaaaggacct gagttcatta ctcaagtcct tttgattaca
    17281 taaattgggc gatgaagacc acgatgatga ttggaatgac gaaacgaaga aggaagagcc
    17341 agacttggaa cagtccttgt ttccatgctc tttcatcgag atggagttcc tccattgcaa
    17401 gagccttttt aaagatatag cctgtaaaaa gtgaaaggaa gagagctcca aatggcatga
    17461 ggagattgga aaccaagaag tccatagcgt caaagaaggc tttaccaaaa atgtgaacat
    17521 ccgccatgac accgtaagat agggctgaag gaatgccaaa gacaaaggtc aaaattccta
    17581 aaataacact ccatttagca cgcttgctgt tgtcctgatt ggtgatattg tctacattga
    17641 tttccagcat aacgacagaa gaagtgacag tcgcaaagag gaagaggaca tagaaaatgg
    17701 ttccaaaagg catcttgtca aagagttgag gcaagacgat aaagaccaag ctcggtcccc
    17761 cttcaatatt cagtctgaag ggggaccgag cttggaaaat ggctagacct gccatgatgg
    17821 ataccgagat attcatggct acgatggaga ttcttgactg gaccagattg gttttcttgt
    17881 ccaagtaaga agcataggtc aacatggctg taacccctag tgagagggca aagaaagatt
    17941 gtctcagagc atagaggaga ccagcgctag tcagttttga aaaatctggt ttgaggaagt
    18001 aaagaacccc ttccatggca tttggcaaac tgagagagcg ttcgatgatg acgacaaaga
    18061 tgataaagag caggggcatc atgactttcg aagctctttc aatccctttt tgaaccccac
    18121 gtgatacaat gaagatattc aataggataa aggtcgcttg agctcctaga gcaatggctg
    18181 gatttgaaat gatcagactt cctgcgaaac aaaatatggc atagtagttc tatgaatgat
    18241 gaagcaagta aacaactaac tgatgcac
//
LOCUS       DAVBCN010000040        17816 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVBCN010000040 DAVBCN010000000
VERSION     DAVBCN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17816
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.040"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            119..778
                     /locus_tag="VQ497_001252"
     CDS             119..778
                     /locus_tag="VQ497_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HET2517558.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRIIEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            803..1870
                     /locus_tag="VQ497_001253"
     CDS             803..1870
                     /locus_tag="VQ497_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2517559.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            1974..2984
                     /locus_tag="VQ497_001254"
     CDS             1974..2984
                     /locus_tag="VQ497_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2517560.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            2996..4687
                     /locus_tag="VQ497_001255"
     CDS             2996..4687
                     /locus_tag="VQ497_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2517561.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            4690..5400
                     /locus_tag="VQ497_001256"
     CDS             4690..5400
                     /locus_tag="VQ497_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET2517562.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
     gene            5496..5864
                     /locus_tag="VQ497_001257"
     CDS             5496..5864
                     /locus_tag="VQ497_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517563.1"
                     /translation="MKDYKINFDLGKIEYFDNNCLIQVYKFISFYDICEMVFAFHLPP
                     DELITNVIFKEKINSMLKCYIDRLLYVFINPTHFTEKVNLQFYGSFFSYEFICREVGN
                     ILKNKGVKCNLNFFEGEEYL"
     gene            5972..6973
                     /locus_tag="VQ497_001258"
     CDS             5972..6973
                     /locus_tag="VQ497_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2517564.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            8156..9517
                     /gene="trpE"
                     /locus_tag="VQ497_001259"
     CDS             8156..9517
                     /gene="trpE"
                     /locus_tag="VQ497_001259"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET2517565.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQAIDRDPLDFLYEVTHKSQHHSDLPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRSVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            9514..10080
                     /locus_tag="VQ497_001260"
     CDS             9514..10080
                     /locus_tag="VQ497_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET2517566.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMICDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            10091..11095
                     /gene="trpD"
                     /locus_tag="VQ497_001261"
     CDS             10091..11095
                     /gene="trpD"
                     /locus_tag="VQ497_001261"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET2517567.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLLSFTPEDLGMEDYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            11092..11859
                     /gene="trpC"
                     /locus_tag="VQ497_001262"
     CDS             11092..11859
                     /gene="trpC"
                     /locus_tag="VQ497_001262"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET2517568.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDC
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNTGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            11846..12445
                     /locus_tag="VQ497_001263"
     CDS             11846..12445
                     /locus_tag="VQ497_001263"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET2517569.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVAADLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            12423..13646
                     /gene="trpB"
                     /locus_tag="VQ497_001264"
     CDS             12423..13646
                     /gene="trpB"
                     /locus_tag="VQ497_001264"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET2517570.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGFGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            13639..14415
                     /locus_tag="VQ497_001265"
     CDS             13639..14415
                     /locus_tag="VQ497_001265"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET2517571.1"
                     /translation="MPKTLTKKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(14387..14488)
                     /locus_tag="VQ497_001266"
                     /pseudo
     CDS             complement(14387..14488)
                     /locus_tag="VQ497_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            14717..14857
                     /locus_tag="VQ497_001267"
     CDS             14717..14857
                     /locus_tag="VQ497_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517572.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WT"
     gene            14894..15448
                     /locus_tag="VQ497_001268"
     CDS             14894..15448
                     /locus_tag="VQ497_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517573.1"
                     /translation="MFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFPL
                     VQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNSN
                     IEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDIP
                     NFSELSETEDFEIIPRIEKQEGQK"
     gene            15463..16614
                     /locus_tag="VQ497_001269"
     CDS             15463..16614
                     /locus_tag="VQ497_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET2517574.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNITIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGNRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(16666..17325)
                     /locus_tag="VQ497_001270"
     CDS             complement(16666..17325)
                     /locus_tag="VQ497_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET2517575.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFRHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLAACVIIFGKLLL
                     V"
     gene            17395..>17816
                     /locus_tag="VQ497_001271"
     CDS             17395..>17816
                     /locus_tag="VQ497_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2517576.1"
                     /translation="MTIRFEEKVSIENAQLVCQWSNSLGKVFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQGLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVR"
BASE COUNT         5726 a         2889 c         3843 g         5358 t
ORIGIN      
        1 cttttaatga aagataaatt aaaaaaaatg tttgacagcg cttgacaaag gaaacggttt
       61 cattgtataa tgtaaacaca atgacaccga tgtcaaatat aaaaaagaag gtctttatat
      121 gaaaaactta acaaaaatta agttcaaaga aaatggggaa tttaatcatt tccctgggaa
      181 tacagttgta gcaaatcttt atactaaaca agatttgatg gaagttgttg atattattca
      241 atcacgttat agagaattac catttatcga taagtttact ttaactccaa ggaattctat
      301 tcacatgact gtaattgaat tgttgtgcca tgaaaatcgt gaaacggagt tttggagcag
      361 taatcttcct ctagatacac ctttacagga aatacatgat tactttgcta aacaacttga
      421 aatttttcca ttgttggatg aagaaattca tatgcgtata attgaaatgg gaaaacaaaa
      481 catactagtt gaacctgcag atgaagcttc tgcaaagaga ttagaagaaa ttcgtactta
      541 tgtctcagaa aaagcaggtg tttgtttccc taatcatgat agatatcaat tccatatttc
      601 aattgggtat cttcggattc ctctaaccga agaggaggaa gaagagttta ctaaagtcag
      661 agcagaatta actgaaattt tattagagaa gattccaact attactgtaa accgtattga
      721 ctatactgta tttgaagata tgaggcaatt tgttccatat cacgaaaaat ttaaataaaa
      781 taataaaaaa ggagaactta tcatgaaaaa aatgaaagtt tggtctactg tacttgcaac
      841 gggagttgct cttactacac ttgctgcttg ctctggaggt tcaaattcta cgactgcttc
      901 ttcatctgaa gaaaaagctg ataaaagtca agaattagtt atctattcga actcagtctc
      961 aaatggtcgt ggtgattggt taactgctaa agcaaaagaa gctggtttta atataaaaat
     1021 ggttgatatc gctggcgctc aattagcaga ccgtgttatt gctgagaaga ataatgcagt
     1081 tgcagatatg gtatttggaa ttggtgctgt tgactcaaat aaaattagag atcaaaaatt
     1141 actagtacag tacaagccta aatggttaga taaaattgat caatctttat cagataaaga
     1201 taattattat aatcctgtga ttgttcaacc attagtttta attggggcgc ctgatgtaaa
     1261 agaaatgcct aaagattgga ctgaattagg tagtaagtat aaaggtaaat attcaattta
     1321 tggtcttcaa ggtggtacag gacgggcaat tctagcaagt atcttagttc gttaccttga
     1381 tgaaaaagga gatttaggcg tatcagaaaa aggatgggaa gttgcaaaag aatactttgc
     1441 aaatgcctat actcttcaaa aaggtgaaag ctcagttgtt aaagcgttag ataaagaaag
     1501 cccaattcaa tacggtatga tctggggttc tggagcgtta attggacaaa aagaacaaaa
     1561 tgttgaattc aaagttatga ctcctgagat tggtgtacca tttgtaactg aacaaactat
     1621 ggttttaagc actagtaaaa aacaagcgtt agctaaagaa tttattgatt ggtttggtca
     1681 atcagaaatt caagtagaat atagtaaaaa atttggatct attcctgcca ataaagatgc
     1741 cctaaaagag ttacctgaag atacgaaaaa atttgttgat caagtgaaac cacaaaatat
     1801 tgactgggaa gctgttggaa agcatttgga tgaatgggta gaaaaagctg aattagaata
     1861 cgtaaaataa gaaataagag ttatttttta tgtccaagtc tctctattat ttctaaattt
     1921 aaggaagaaa taaagagagg cttttggttt tagtaataga gggagttagt gagatgatta
     1981 aatttgataa tattcaaatt aaatatggtg attttgttgc aattgataat ctgaatttag
     2041 atatacatga aggggaattc tttacatttc ttgggccttc aggatgtggt aaatcaacta
     2101 ctttgagagc attggtaggt tttttagatc catcatcagg aagtattgaa gttaatggaa
     2161 cagatgtcac tcatttggaa cctgaaaagc gtggaattgg tattgtattt caatcttatg
     2221 cgctatttcc aactatgact gtttttgata atattgcatt tggtttaaaa gttaagaagg
     2281 tagctccaga tgttattaaa gctaaagtat cagcagtggc agcaaaaatt aagatctctg
     2341 atcaacagtt acagcgtaat gtatcagaat tatctggggg tcaacaacaa agggtagcat
     2401 tggctcgtgc tctggttctt gaacctaaaa ttctttgtct agatgaacca ttgtcaaacc
     2461 ttgacgcaaa attacgtgta gatttgagaa aagagttgaa aagacttcaa aaagagttag
     2521 gtattactac tttatatgtt actcatgatc aagaggaagc cttgacttta tctgatagaa
     2581 ttgcagtctt taacaatgga tacatcgaac aggtcggtac accagtagag atttatcata
     2641 attctcaaac tgaatttgta tgtgatttta ttggagatat taatgttttg accgatgaaa
     2701 cagtccacga agtattactg aaaaatacaa gcgttttctt agaggataaa aaaggttaca
     2761 ttcgattaga gaaagttcga ttcaatcgtg aaactgaaca agattttatt ctaaaaggga
     2821 caattattga tgttgagttt tctggagtta caattcacta tacaataaaa gtttctgaaa
     2881 gtcagattct taatgtaaca agtattgata gtcaggctgc tattagatct gtcggagaaa
     2941 gtgtggaatt atttatcaca ccatcagacg ttctgcaatt ttaaggaggt gcagtatgcg
     3001 tcataaatta aatttaaaag attggcttat tcgtttaggg ttaatctggt tcttagtaac
     3061 atttattatt tatccaaact ttgatctagt agtgaatgta tttgtaaaag gaggagaatt
     3121 ttcccttgat gctgtacatc gtgttctaaa atctcagagg gcacttcaga gtattatgaa
     3181 cagttttaag ttagcatttt cactcattat tacagttaat gtcgtaggta ttctttgtgt
     3241 tctatttaca gagtactttg atattaaagg tgctaaaatt ttaaaattag gttatatgac
     3301 ctctttaatt tatggaggag tggttttagc gactggatat aaatttgtct atggtcctta
     3361 tggattgatt acaaaatttt tacaaaatgt tatcccttct ttagactcta actggtttat
     3421 tgggtatggt gcagtcttat tcattatgac attttcagga actgctaatc atacattgtt
     3481 tttaacaaat acaattcgaa gcgttgacta tcacactatt gaggctgctc gaaatatggg
     3541 agcaaaacca tttactgttt tccgaaaagt agtgttacca accttaattc caactctatt
     3601 tgcacttact attatggttt ttcttagtgg tttatctgca gtagcagcac ccatgattgt
     3661 tggtggtaaa gaatttcaaa ctataaatcc aatgattatt acatttgcag ggatggggaa
     3721 ttctcgtgat ttagctgcct tacttgcaat tattttaggt attgcaacta caattttgct
     3781 tactatcatg aataagatag aaaaaggtgg aaattatatt tctatctcta agactaaagc
     3841 gcctcttaaa aaacaaaaaa ttgcgtctaa gccttggaat atcattgctc acattgtagc
     3901 atatggattg ttcacagttt tcatgcttcc actaattttt atagtattat actcatttac
     3961 agatccagtt gcaattcaaa caggtaactt aacattatca aactttactt tagaaaatta
     4021 tcgcttattc tttagtaata gtgcggcatt ctctccattc ttggtcagct ttatttattc
     4081 tattattgct gcgacaacag caacaattct cgcagttgta tttgctcgtg ttgtcagaaa
     4141 acataaatct cgttttgatt tcttatttga atatggtgct ctacttcctt ggttactacc
     4201 aagtacactt ttagcagtaa gtttattatt tacttttaat cagccacaat ttcttgtctt
     4261 gaatcagatt ttggtaggta gtttggtaat tctacttatt gcatatatag ttgtaaaaat
     4321 cccattttct tatagaatgg tacgtgctat tttatttagt gttgatgatg agatggaaga
     4381 tgcagcaaga agtatgggtg cttcaccttt ttatactatg atgaaggtta tcattccatt
     4441 tattttaccg gttgttctct ctgttattgc tttaaacttt aactctttat taactgactt
     4501 cgacttatct gtattccttt accaccccct agctcaacca ttaggtatta cgattcgatc
     4561 tgcaggtgat gaaacagcaa catctaatgc acaagctctg gtatttgttt atacaattgt
     4621 tctgatgatt atttctggaa cggtattata cttcacacaa agaccggggc gtaaagtaag
     4681 gaaataatca tgacagccac tagtcttggg ttatcaaata ttgaaatagt tgtcaggatt
     4741 gttttatcag tagtcattgg tagtataatt ggtttagaga gagggagcaa atcccagcct
     4801 gcaggcatcc gaacttatag tattgtttgt ctagctgcat gtttgattat gatgacgaat
     4861 gaatacgtat cttataaatt tgggacagga gatcctacac gattaggagc tcaagttata
     4921 tcaggtgtgg gttttctagg cgctggaacg attcttatta cagataaaaa gaaaattaca
     4981 ggtctgacaa ctgcagcagg catttgggct tcggcaggaa ttggattagc tattggagta
     5041 ggtttttatg agggagctct tttagtagcc atttctgttt ggggtgtgat atccatgttc
     5101 caaccactaa aaaaatatct acaaaatcgt tctaaaatga ttgaattgta tatagtagtt
     5161 aaatctacag aagcctataa tcgtgtattg gtttattgtg cagaaaatgg tatcagaatg
     5221 accgattcaa gaacagcgtt tggagatgtt aattctgaca gaattgaata ctttgatgtt
     5281 ccagataaaa aaatagcgtc atttattact ctggaattat caggtagatt tgaacatctt
     5341 cgactgatgg aagaaattgc taatattgta ggtgttattt atgtcgaaga aattagctga
     5401 aatttagtaa acaaagggac agatgttttt gtccttttgt tttttagata ttttaatcat
     5461 ctaatttttg agtagatttt tgctcagagg tttttatgaa agattataaa atcaattttg
     5521 atttaggtaa aatagaatat tttgataata attgtttgat acaagtatac aaatttattt
     5581 ctttttatga tatttgtgaa atggtgtttg cctttcattt acctcctgat gagttgataa
     5641 caaatgttat ctttaaagaa aaaattaact ctatgttaaa atgttacatt gacaggctat
     5701 tatatgtttt tataaatcct acacatttta ctgaaaaagt taatctacaa ttttatggta
     5761 gttttttctc atatgaattt atttgtcgtg aagttggaaa cattcttaaa aataaaggtg
     5821 tgaaatgcaa tttaaacttt tttgaagggg aagaatattt gtaaaattaa ttttgtgaat
     5881 ttgtggatct taaataattt actaaattct cttgttaaaa aaaattaaat attgtatagt
     5941 aatattagaa tgtttgtaat agaggactga aatgaaaaat ataaatggga aaaaggtaac
     6001 tatttatgat attgcacgtt tatcaggttt ttctccaaaa acagtttcac gagttattaa
     6061 tggtggagta aatgttaaag aagaaactta tcaagcgatt caaaaagtta tagaggaatt
     6121 gtcgtatatt ccaaatgcgt atgcaaaaaa tttaactaaa aaagaagcta taaatatatt
     6181 gatttctgta aaaaaaatcg actcttttcc tttaatttgg tttcatacgc tgttagataa
     6241 agtgttacgg acgtgtaaag aatttggtgt taatgctata gttgagtact ttggagagga
     6301 ggatacaatt agtaattcaa ttatatcaag cacaggtagc ctagtagatg gtgttattgt
     6361 tttttatgaa agtgtagatg atatcagaat tcagtattta aagaagaacc atatgccttt
     6421 tcttgttttc ggtgaatctc aaacgtctgg agtagtctat gtatctaata ataattttca
     6481 agctacttat gatatgatga aagctgtaac tgaagaaaaa tttaaaaata tgtggttgct
     6541 tatgggagga gaatcccatg ttaataagga tcgtgaaaga ggtgtgcgtt cttttctgaa
     6601 tgataaaaat tactttatgg atttaaaagt catttatggc ttatctacaa ttgattcagt
     6661 ttattcttat gcaatgcagc acttgactac ccaaaattat ccggacatta tttttgtttc
     6721 aggagatgaa aaggttcagg gcttgattcg tgcgtgctat gagaaaggaa ttcttattcc
     6781 agatgatatt tctatcattg gatttgataa tattcctatt tctcagtatt atactccagc
     6841 tttgtctaca attgctccca attatgttaa attggctaaa gaaatgatag aaggtgtttt
     6901 agcaattatt aaaggggaaa gtgtcacatc tgttgaagtt tctcctaaat ttgttaggag
     6961 acagagtttc tagtattgtt taattcttga aaatcctagt ggaaaagatg gagtgttcta
     7021 aaataagttc tgtttagcct ccattcagtt tctattgagt tggatccaat ttctaacaag
     7081 tgggacatgt tcatgttcga tctaaggaag atatggtcac attcgaatga tatcaaaaaa
     7141 atcgtaaaaa agtcttgaca aagaaaaaac aaagtattaa aataagttca acaaatcaga
     7201 aaagtaacta tttttgatct tcagggagcc tgtggtgatt gtgaataggt ggttggaagt
     7261 agtgaaagtg ggctgatttt gaaaatgaat ttgaaacaat gaaaattcgg tgcgcacacc
     7321 ttacagtgca gcttgttgtt aaacaaggca gagatgtaaa gggatagtcc ctttataatt
     7381 gaggtggcac cgcgttacca acgccctcac acggaagtat attctgtgtg tgggcttttt
     7441 tctatccgtc gtttggttta tcttttatta gggccttaag tcgctttgat gaacttgagt
     7501 tctatctaca gctccttgcc tcctactaaa agtaaacaaa aaggctggac taatacgaaa
     7561 aaagctagta atactcaatg aaaatcaaag agcaaactag gaaactagcc gcaggttgct
     7621 caaaacactg ttttgaggtt gtggatagaa ctgacgaagt cagctcaaaa cactgttttg
     7681 aggttgcaga tagaactgac gaagtcagta accataccta cggcaaggcg acgttgacgc
     7741 ggtttgaaga gattttcgaa gagtataaag gtgtaccgag atgaacatct cttcatcaat
     7801 gtctctggtc gtggaggcaa ggacctagct gcgattgcag attactagaa gctaaaaaat
     7861 aatagatgga aaaattacag tcttttctct cacagagagc ttgtggttgc tgaaaacaag
     7921 cagagaaagc tgtaaagttg ggttcatctg aaacgagaga agaaaacatc attctcaagg
     7981 gagtgccctt tatcgcacag cctgttacag gagatatctg agacaggaat gagagatagg
     8041 agaaatccta taattgaggt ggcaccgcga atttcgtcct cacgcaagtt attttgcgtg
     8101 gggatttttc atacagtcgc cacgaactag aattagaact gaaagggaaa tgacaatgga
     8161 acgaatcatt catggagatg tcttatcacc aatcttggct tatatgcgct taaaggggca
     8221 acataaggtt attttagaga gtattccgag agacaaggaa acagctcgtt tttctatcct
     8281 agcctataat ccagtttttg agattaagtt tgaaaatgga gttctctatc aaaatggtca
     8341 agcgattgat cgggatccct tggatttcct ttatgaagtg actcataaga gccagcacca
     8401 ttcagacctc ccttttggtg ggggagccat tggttttgtt ggttacgata tgatttcgct
     8461 ttatgaagaa attggtcaaa tccctgagga tacaattggg acgccagaca tgcatttctt
     8521 tgtctatgag agctatatgg tctttgacca caagaaggag aagatacatg tcatagagga
     8581 tgcactttat agtgagcgca gtcaagaggc cttggaaaaa tccttgaacc aagtgcttga
     8641 ggaattacgc attcctgctc caaatgaatt tgaagacttg gatttatcac cacttgactt
     8701 caaaccgcat atcgctccac ataagtttga gggaatggta gaaacagctc gtgacttgat
     8761 tcgtaacgga gatatgttcc aatgtgtact cagccagcgt ttctcagcag aagttactgg
     8821 aaatccattt gacttctaca gaaatctccg cgtgactaat ccatctaatt acctttattt
     8881 ctatgatttt ggggattatc aaatcatcgg agctagtcca gaaagtttgg tttctgtcaa
     8941 aaatggcatc gtgacaacca atccgattgc agggacgcga ccaagagggg ctacggatga
     9001 agaagacaag gccttggcga ctgacctcct ctctgatgag aaggaaacag cagaacatcg
     9061 aatgttggta gacttggggc gtaacgatat tggccgcatc tctgaaacgg ccagtgtcca
     9121 agttactaag tatatggagg tggagctctt ccgctacgtc atgcatttga ccagtgtggt
     9181 caagggacgt ttgcttgcag aactcactgc catggatgcc ttgaaagcaa cgcttcctgc
     9241 tggaaccgtt tctggagcac caaaaattcg agctatgaga cgcatctatg aactggaaac
     9301 ggaaaaacgg agcgtatatg caggagcaat cggctacttg tctgcgacgg gtgatatgga
     9361 tttggccatt gccatccgaa ccatgattct caaaaatcaa agagcctatg tgcaggctgg
     9421 ggcagggatt gtctacgact ctatcgccca aaacgaatac caagaaacca ttaacaaagc
     9481 aaaatctatg actagaattg gagaactaag accatgattt tattgattga caactatgat
     9541 tcttttacct ataacttggc ccaatacatt gggaattttg cagaagttca ggttttgaga
     9601 aatgatgatt ccaagctgta tgaagaagct gaaaaagcag atggtctggt cttttctcct
     9661 ggtcctggtt ggccagttga tgctggaaag atggaagaca tgatttgtga ttttgcaggc
     9721 aagaagccga ttctagggat ttgtttgggt caccaagcca ttgcagaagt ctttggtggt
     9781 aagctaggtt tggctccaaa agtcatgcat gggaaacaga gcaatatcaa ctttgaagcg
     9841 ccatctgttt tgtatcaagg tattgaggat ggacgtgcgg tcatgcgtta tcacagtatt
     9901 ttgattgagg aaatgccaga agactttgaa gtgacagctc gttcgactga tgaccaagcc
     9961 atcatgggga ttcaacataa aaacctaccg atttatggct tccagtacca tccagagagc
    10021 attggaacgc cagacggctt gtcttctatt cggaatttta tcgagaaggt tgtaaagtga
    10081 ggaaactagg atgaaagaga ttattgaaaa actagcaaaa tttgaaaatt tatcaggtgt
    10141 ggaaatgacg gatgtcattg agcgtatcgt aactgggcgt gtaactgaag cgcagattgc
    10201 ttctctcctc ttagctctta agatgaaggg ggaaacacct gaagaacgta cagccattgc
    10261 ccaagtcatg agaggacatg cccagcatat tccaactgag attcatgatg ctatggacaa
    10321 ctgtggtaca ggtggggaca agtctttcag ttttaatatt tccacaactg cagcctttgt
    10381 cttggctggt ggcggtattc acatggccaa gcatggtaac cgctcgattt cttctaaatc
    10441 tggttccgca gatgtcctcg aagccttggg aatcaatctt gacctcaaac cagctgaact
    10501 aggtaaggtc tttgataaaa ctggaatcgt ctttctcttc gctaaaaata tgcacccagc
    10561 tatgaaatac atcatgccag ctcgtttgga attgggaatt ccaacgatta tgaacttgac
    10621 tggtccactg attcatccaa tggctttaga aacacagctt cttggaatta gtcgtccaga
    10681 actcctagaa agtacagctc aggttttgaa aaatatgggt cgcaaacgtg ccatcgtggt
    10741 tgctggacca gaagggttgg atgaagctgg cttgaacgga acaaccaaga ttgcacttct
    10801 tgaaaatggc gaaatcagct tgttaagctt tactccagag gatttgggaa tggaagacta
    10861 tgctatggaa gatattcgtg gtgggaatgc tcaggaaaat gcagaaattt tgcttagcgt
    10921 tctgaaaaac gaagcaagtc cattcttgga aacgacagtc ttgaatgctg gtcttggttt
    10981 ctatgctaat ggtaagattg atagcatcaa ggaaggagtt gccttggccc gtcaagtgat
    11041 tgctagaggc aaggcccttg aaaaactcag actgttacag gagtaccaaa aatgagtcag
    11101 gaatttttag cacgaatctt agagcagaag gcgcgtgagg tggagcagat gaagctggag
    11161 caaatccagc ctctgcgcca gacctatcgc ttggcagaat ttttgaagaa tcatcaggac
    11221 tgcttgcagg taatcgctga ggtcaagaaa gctagcccta gtttgggaga tatcaatctc
    11281 gatgtggata ttgtgcaaca ggcccagact tatgaagaaa acggagcagt gatgatttcg
    11341 gtgttgacag atgaggtttt ctttaaaggg catttggatt atctacggga aatttccagt
    11401 caggtagaga ttccgacgct caacaaagac tttatcatag atgaaaagca aatcatccgc
    11461 gctcgcaata caggtgcgac agttatcttg cttattgtgg cagccttgtc cgaagaacgc
    11521 ctcaaggaac tgtatgacta cgcgacagag cttggtctgg aagtcttagt ggagactcac
    11581 aatctagctg aactagaggt agcccacaga cttggtgctg agattatcgg ggtcaacaac
    11641 cgcaacttga ctacctttga ggtcgacttg cagaccagtg tagatttagc cccttacttt
    11701 gaggaaggtc gctattacat ttctgaatct gccattttca cagggcagga tgcggaacga
    11761 ctagccccat actttaacgg aattttggta gggacagctc ttatgcaggc agaaaatgtg
    11821 gcccagagaa tcaaggagtt gcagattgac aaaggttaaa atttgtggac tatcgaccaa
    11881 agaagcggtg gaaacagccg tttcagcagg agccgactat atcggttttg tctttgcacc
    11941 tagtaaaaga caggtgactt tagaagaggc agctgagttg gcaaagctta ttcctgcaga
    12001 tgtgaaaaag gttggagtat ttgtttcacc aagtcgggta gaactgctgg aagcgattga
    12061 caaagttggc ttggacttgg ttcaagttca cggtcaggtg gcagctgatt tatttgagaa
    12121 tttgccttgt gccagtattc aggctgtgca ggtagatgga aatgggcatg tccccaattc
    12181 tcaggcagat tatctactct ttgatgcccc tgtggcagga agtggtcagt cctttgattg
    12241 gggtcaactg gatacgactg gactagcaca gcccttcttt atcgcaggtg gccttaatga
    12301 agataatgta gtaaaagcaa ttcaacattt tactccctat gcagtagatg tatcgagcgg
    12361 agtggagaca gatggacaaa aagatcatga aaagattaga agatttatag agagggtaaa
    12421 gcatggcata tcaggaacca aataaagatg gattttacgg aaaattcggc ggacgttttg
    12481 tcccagaaac attgatgaca gcagttttgg agttggagaa ggcctaccgt gaaagtcagg
    12541 cagacccaag tttccaagag gaattaaacc agcttttgcg ccagtacgta ggacgtgaaa
    12601 ctcctcttta ttacgcaaaa aacttgaccc agcatatcgg cggagccaag atttatctca
    12661 aacgggaaga ccttaaccat acaggagccc acaagattaa caatgccttg ggacaagttt
    12721 ggcttgccaa acgcatgggt aaaaagaaaa ttatcgcaga aactggtgct ggtcagcacg
    12781 gtgtggcaac tgcaactgct gcggccctct ttaacatgga atgtaccatc tacatgggtg
    12841 aggaagatgt caaacgccaa gccctcaatg tcttccgtat ggagcttttg ggagctaagg
    12901 tcgaggccgt gacagatggt tcgcgcgtgc tcaaggatgc ggtcaatgca gcccttcgtt
    12961 catgggtggc taatatagat gatacccact atatccttgg ttctgccttg gggcctcatc
    13021 catttccaga aattgttcgt gacttccaaa gtgtcattgg tcgagaggct aaacaacagt
    13081 accgtgactt gacaggacaa aatctgccag atgccctagt agcctgtgtt ggtggtgggt
    13141 cgaatgccat cggtctcttc catccctttg tagaagatga gtcagtagcc atgtatggag
    13201 ctgaagcggc tggatttggt gtggatacgg agcaccacgc agctaccttg accaagggtc
    13261 gtccaggtgt ccttcacggt tccctcatgg atgtgctcca agatgcccat ggtcaaattc
    13321 ttgaagcctt ctctatctca gcaggtttgg actatcctgg tatcggtcca gagcattctc
    13381 actaccacga tattaaacgt gccagctatg tccctgtgac agacgaagaa gccttggaag
    13441 gattccaact cttgtctcgt gtggaaggga ttatcccagc tttggaatct agccatgcta
    13501 tcgcctttgc ggtgaaattg gccaaagaac ttggaccaga aaagtctatg attgtatgcc
    13561 tatcaggtcg tggggacaag gatgtggttc aagtaaaaga ccgcttggaa gcagatgcag
    13621 caaagaaggg agaagctcat gcctaagaca ctaacaaaaa aattaaatgc tattaaagca
    13681 gctggaaaag gaatttttgt tccctatatc atggctggtg accacgaaaa aggtttggat
    13741 ggtcttgctg aaacaattca ctttttagaa gatttgggtg tctctgcaat tgaagtgggt
    13801 attccctttt cagaccctgt tgcagatgga cctgttatcg aagaagctgg cttgcgcagt
    13861 ttagcccacg ggacctctac ccaggcttta gttgaaacct tgaaaaccat tgaaacagag
    13921 attccactgg tcatcatgac ctacttcaac cccctctttc agtacggtgt ggagaacttt
    13981 gtcaaagatc tggctgatac agctgtgaag ggcttgatta tcccagacct gcctcatgaa
    14041 catgccaact ttgtagaacc atttttggca gatacagata tcgccttgat tcctttagta
    14101 agcttgacca caggaattga gcgccagaaa gagttgattg aaggggcgga gggattcgtc
    14161 tatgccgttg ccatcaatgg ggtaacaggg aaatcaggca attaccgtgc agatttggac
    14221 aagcacttgg cgcaacttca tcaagtggcc gacatcccag tcttgacagg ttttggtgta
    14281 tctagtcaag ccgatctgga acgcttcaat gcggtgtcag atggtgttat cgtcggttcg
    14341 aaaatagtaa aagctctcca ccaaggagag ccgattcagg actttatcaa acaagcagta
    14401 gcttaccaaa aataattaaa caagtagcaa gtaagaggaa aggcacgaaa ggaagtcttt
    14461 cctttttctt ttgcaggcga aaggctagtt ttatagtgaa ttgaatctgg aatattacac
    14521 tacggttcct aaaatatttt aagaaagtga tttgaatttc ccaataaatt tgttcagttt
    14581 ttaattgatt ttactataga agatataatt ttctaaatta ctctgacaca gaactttaag
    14641 aaaaagctaa ttattgtagt aatttcttta agaaaatgat ataattcatt aaaaagtatt
    14701 tggaggagga agaattatgt tgaatcagga tctctttgat tcgcttgagg cacaaaaaat
    14761 tgtagatact ttgatgaaag ggcaaaaaga ttatgtagat gaacgtctag aaaaaagaga
    14821 gacaatgata gtgtcgaatg gttatgcatg gacgtgacct aatcatattg atactgcatt
    14881 tgcatcagca gatttgtttg agtataaatt acaattagca ggacagactt ggggatattt
    14941 agaatttgaa acaaatacag aaaaatatgg gaaagtattg ttaattataa agggtaagaa
    15001 gcgacttacg aaccaatttc ctttggtaca aaaaaataag agtggctact tatttgaata
    15061 tgctcagatg aatacacttt atcttaatca acattcttcc tacaaaaatg atgaagatag
    15121 tcattccttt ccaattcaga tggagttagt ttctgatgaa atgattcaag aaattgaaca
    15181 agctactaaa aattcgaata tcgaaaaatt tatgatttta acttatgagg cggactcaga
    15241 aaacaatatt atatctgtag atgttgttat gcctgatgca cgaactggtc agttacactt
    15301 gattcaagat ttgtctgagt atattcaatc aagttcgtac catttcgaag aagctaaata
    15361 tcaagatatt cctaattttt cagaattatc ggaaacagaa gattttgaaa ttattccaag
    15421 aatagaaaaa caagaaggtc aaaagtagta aggaatatgt aaatgtttaa tggtcgggta
    15481 ttgaaagaat tacggctgtt aaatggttta agtagagcag aattagctca gagaattaat
    15541 ttaacggaac aagccatttg gcagtttgag tccaacgaaa cgaaacctaa attatcaacc
    15601 aaaatgcatt tggccaacca atttcatgtt gatttaactt attttgaaca ggaagaagag
    15661 agcattcgat ttgattcttc tgtaattgcc tttagaaatg cagacctagc aacacggaaa
    15721 acaatagata ttcaaactat gtatttacat aaggtagata gtttgattga ttattttgaa
    15781 agttttgtaa ttatacctaa tattacaatt catgacctaa gtaatgtagt gagtgaatct
    15841 tatcataagg gagaatccat tgaggaattg gctctttatg ccagggaaaa attaggtatt
    15901 tcaaaagata atcatgattt gctttataaa ttagaacgtt caggcatcta tatcgtggaa
    15961 cgattaatta atggccaagc tgatgcttat agcgcatggt caaaattggg aagaccttat
    16021 attgtgttgg gaacgaataa atcatctgta cgtcgaaatt ttgacttagc tcatgagcta
    16081 ggacatattc ttttacacaa atataaagat atgaatgaag atggcaatcg tttggagcaa
    16141 gaagcaaatt attttgcatc atgtttttta ttgccaaaag aagagttttt agtcaaattt
    16201 gaagagaggg ttggcaagcg tgtcagcaat cctgatagtt atattttatt gaagtcggat
    16261 ttgaatgttt cgatacaggc tttagagtat cgagctttta agttaggatt attgactcca
    16321 aagcaacatt cttactttta tcgtcaaatt gcgcaaaaag gttacaaaat gattgaacct
    16381 ttggatgatc aaatttttgt taaaaaacca agcaaagtaa agagtattct ggacgtcgtt
    16441 ttgagtaatc atctagtcag tctagcgact ataatgtcta aacaaagtat tcgtttacag
    16501 tttataagcg aaatattttc agtcgaaatg aaattttttg atcagtatca agaagataga
    16561 agaacagatc gatttgataa catcatccct ttgtacaaaa gaaataattt ataatttaac
    16621 tgtgaaataa tttcatgaag gacatatggc agaaattgga ttttatcaga caagcagtag
    16681 cttaccaaaa ataatcacac aagcagcaag taagaggaaa ggcacgaaag gaagtctttc
    16741 ctttttcttt tgcaggagaa aggccagaat acccgtcgca gaagcgaact gaatcaagat
    16801 cagtaactcc gttacgctaa agacgagagc acaagaagct aaaaagagga aatctcctgc
    16861 gcccatgcgg atatcgataa aatgagccaa aattccaaga gcaaggaagg agaccatgac
    16921 cagattccag ccagaggaag ctattaggat taggtggaaa gtcatccaga ccagtaaggg
    16981 atattcctga tggcgaaagt cgtagatgcc taaggtcaaa ccagcggtga ttaggacgac
    17041 ttgccccaag gaaagcaatt cccaagagta aagcagaaag aggagtccta agactaattc
    17101 aaagagggca taccagacag gatagcgaac tttgcagtag cgacagcgaa agcgattgaa
    17161 gacctgtgag agaatcggaa tcaaatctaa gggacgcaag cgagtctgac aggaatcgca
    17221 gtgactggct gaactgataa tggattgctc tggaaaacgg tcaatgacca aaccaagaaa
    17281 ggaagcgaga atgctcccga cgagaaaaaa ataaaaatca atcatactta tctattcgta
    17341 aaaaatggga gaaatagtat aatggagaaa catagataag atggtgagga aaagatgaca
    17401 attcgttttg aagaaaaggt gagtatagaa aacgctcagc tcgtatgcca atggtccaac
    17461 tccttaggca aagtctttca agaacaatgg atgggaccaa ggattccttt tctactgacc
    17521 cttcaagcct tggaaggagt cttttcaatc tttgatgaac aagagtttgt gggacttatc
    17581 cagaaaatca ggctagaaga cagcaatctt catatcggga gattttttat caacccccag
    17641 aaacaggagc aaggcttagg tagccaggct ttaaggaaat ttgttagttt ggcctttgaa
    17701 aatgaagata tagatagtat ttctctaaat gtcttcgagg caaatcaaag agctcagaat
    17761 ctttaccaaa aagaaggatt tgaaatcgtt caaatggttg aagcacctgt acgaaa
//
LOCUS       DAVBCN010000041        17710 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVBCN010000041 DAVBCN010000000
VERSION     DAVBCN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17710
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.041"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(114..1964)
                     /locus_tag="VQ497_001272"
     CDS             complement(114..1964)
                     /locus_tag="VQ497_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET2517577.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMVDFWGLSASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVARAVDEAKVPKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(2032..3171)
                     /locus_tag="VQ497_001273"
     CDS             complement(2032..3171)
                     /locus_tag="VQ497_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2517578.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKGKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDAEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGGLILKIDAHSKVTESFVMNNVAVIQQGEFVCGGPRP
                     TIVKGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLSLLTLLTLLKHK
                     NGFLIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKGTVIYLEK"
     gene            3229..4395
                     /locus_tag="VQ497_001274"
     CDS             3229..4395
                     /locus_tag="VQ497_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HET2517579.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTRLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TAMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIDILMTVALFTVDRADKIL"
     gene            4534..4860
                     /locus_tag="VQ497_001275"
     CDS             4534..4860
                     /locus_tag="VQ497_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET2517580.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            4847..5440
                     /locus_tag="VQ497_001276"
     CDS             4847..5440
                     /locus_tag="VQ497_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HET2517581.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEVAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            5433..6344
                     /locus_tag="VQ497_001277"
     CDS             5433..6344
                     /locus_tag="VQ497_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HET2517582.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            6407..7471
                     /locus_tag="VQ497_001278"
     CDS             6407..7471
                     /locus_tag="VQ497_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517583.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIETPEGGEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(7543..8403)
                     /locus_tag="VQ497_001279"
     CDS             complement(7543..8403)
                     /locus_tag="VQ497_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HET2517584.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHIYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTETDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLEVLPPSFYHQLDANQIEVQPILD
                     KDFKTLAQEK"
     gene            complement(8525..9511)
                     /locus_tag="VQ497_001280"
     CDS             complement(8525..9511)
                     /locus_tag="VQ497_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HET2517585.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(9783..11258)
                     /locus_tag="VQ497_001281"
     CDS             complement(9783..11258)
                     /locus_tag="VQ497_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HET2517586.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(11362..12285)
                     /locus_tag="VQ497_001282"
     CDS             complement(11362..12285)
                     /locus_tag="VQ497_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2517587.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(12299..13228)
                     /locus_tag="VQ497_001283"
     CDS             complement(12299..13228)
                     /locus_tag="VQ497_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2517588.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(13547..13897)
                     /locus_tag="VQ497_001284"
     CDS             complement(13547..13897)
                     /locus_tag="VQ497_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517589.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETADKDVLENSQEELQKAVLFLADEKGSEHTEAELI
                     DNLKEVIAKLKANA"
     gene            complement(14323..14478)
                     /locus_tag="VQ497_001285"
                     /pseudo
     CDS             complement(14323..14478)
                     /locus_tag="VQ497_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(14664..15275)
                     /gene="rpsD"
                     /locus_tag="VQ497_001286"
     CDS             complement(14664..15275)
                     /gene="rpsD"
                     /locus_tag="VQ497_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET2517590.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(15470..16522)
                     /locus_tag="VQ497_001287"
     CDS             complement(15470..16522)
                     /locus_tag="VQ497_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HET2517591.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVTKLEKGQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(16519..17217)
                     /locus_tag="VQ497_001288"
     CDS             complement(16519..17217)
                     /locus_tag="VQ497_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2517592.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(17384..>17710)
                     /locus_tag="VQ497_001289"
     CDS             complement(17384..>17710)
                     /locus_tag="VQ497_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2517593.1"
                     /translation="VKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMTSS
                     TNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLGLK
                     NKKEEN"
BASE COUNT         5253 a         3828 c         3281 g         5348 t
ORIGIN      
        1 acggcaaggc gacgttgacg tgatttgaag agattttcga agagtataag tctctacata
       61 ataaaacgca tattaccaag gttatttaac ttgataatat gcgtttttct tttttattca
      121 atgtgggttg tttgattagc aaaggcgata atagcttgct tcaactcatc tccactgaga
      181 gtgcggaact ctccaatctt ttgattgatg gattctaaag gcatgacatt aaccttacca
      241 acgaaaatct tatccataac ttgattatca acgagttcgg ttgataccaa gagttcttcg
      301 tagagttttt cacctgggcg gattccaact tcaacgattg gaatttcact ttcagtgtgg
      361 ccacttagaa gcaccatctt cttggccaag tcataaatct tgactggttt gcccatatca
      421 aggataaaga cttccccatc tttggcataa gcaccagcat ggataaccag acggctagct
      481 tctggaatgg tcataaagta acgggtcatg cggaagtctg tcaccgttac aggcccacct
      541 tcagcaatct gacgttcaaa gactggaatg acactaccac ggctaccaag aacattccca
      601 aaacgaactg cacagtaggt tgattggcta cgttggttaa agccagtgac aatcaactcc
      661 gccacgcgct tggttgctcc cataacattt ggtggattga ctgccttatc tgtcgaaatc
      721 ataaccatct taggcacctt ggcttcatca acggctctag caacattgta agttccacgg
      781 atattgtttt tgaaggcttc ttttggattg cgctccatca taggaacatg cttgtgggct
      841 gccgcatgat aaacaatagc aggtttgtac tgctcaaaga cttgcaacaa acgatcatag
      901 tcttgaatgt ccgcaatcac aggtacataa tcaatccctt ggaacttacg aatcaattca
      961 tgataaacaa ggtagattga gttttcccca tgaccgagca agacaatgcg ttcaggattg
     1021 aagcgactaa cttgacgaca gatttcagaa ccgattgaac ctccagctcc tgtgactaag
     1081 atggtcttac ctgtcagttc tgcacccaga cgcgattcgt caagacggat ttcctgacga
     1141 cccaaaaggt ccgtaatatc aattttttgg aagccagtac ctgcttggtg aaggccctga
     1201 acaacagttt caaccttagg catcttgtaa catttgacac ccagcttatt acacatctgc
     1261 aagatacgct catattctga cggatccagc gacggaatcg caacgatgac acgctcgatt
     1321 tgatggcgtt tggctaattc aggcagattg tcataagagc ccaaaacagg aataccacca
     1381 agtttttgac cctttttctt agaatcctta tccaaaatac cgaccagttc taattcactg
     1441 gttggatgtt ggtaactatc cataaagaga gccccaccat caccggcacc aatcaagaag
     1501 gtccgacggt gttctccatc accactacct tttttgcgtc tggagtagat taactgccaa
     1561 gtaatccgtg gcaataaaat caagaaggta ctcaacaaga taaagagaat gataaaacgg
     1621 atggagaaga gtggcaagaa ggcataacag atactatatg acaagacact gctagcagtc
     1681 acaccaaaaa agattttcat gaaatccgta atcttgctgt aacgactaat actcgcgctc
     1741 aagccccaaa aatcaaccat caattgatag aacaggaagg ccaaactcgt atagataatg
     1801 tagtcaacag gtgctggatt aatcagccca taaaataaaa tataagatac aatgatggaa
     1861 accaccatac tgaaaatatc aaatattccc cagaaaacct gcttttgttg tttatttaaa
     1921 atttccacca gatcaatcac ataatctgtg agttttttat tcatagaaat ttactcctcc
     1981 ttaaaatagt tctactatat tgttattata gcacatttta atttatttgg cttatttttc
     2041 caaataaatt actgttccct tgtactcctt cttccattta aatcctctaa ttaaacctac
     2101 aatcgtccca aggccataag caaagtgaat ggaaaataaa agaaagggca tcacaattag
     2161 aaatccattt ttatgtttta ataaagtcag caaagtgagt aatgacagta atgataaaag
     2221 tagaaaatag gcacctaata gtaaagttat gaatacgaat gtgatcggta acaatgctag
     2281 actaaacaca agactcaaaa caaataaaca aggaacataa tgaaataatg ataaacactt
     2341 aaactgaaca tgacttgtca agccaatcca caaaccattt gaatactttt gatgcagcat
     2401 tttcttgaat gttggtcgaa tatactgata agatagaata cttgggctat agcggatttt
     2461 ataaccatgt tctcgaattc tataatgaat atcattatct tcagttcggc caagttgctc
     2521 atttactaaa ccaaccttct ggaaaacctc tcgtttatac atcccatgaa aaatagaaga
     2581 aacatatcta tcctcggaac tatttcgata attggcaatg ctactgccaa acatattttc
     2641 ctcaacaaga tgcaaggtct ctgcccattt tccttttcct ttgacaatcg tcggtctagg
     2701 ccctccacag acaaattcac cttgttgaat aacagccaca ttgttcatta caaaactctc
     2761 agtaactttt gaatgagcat caatttttaa aataaggccc cctacagaat gcttaactcc
     2821 caggttaaaa ccactagctt gatttttctt aggattgtta tacaatctaa ttgagttaaa
     2881 ctctgcatct tcttttataa attgctgaat gatagctgtg gtcccatctg tggacatagc
     2941 atttataaat agaatttcaa tattctcttt aggataggtt tgatttttta agtcttcaat
     3001 tagaccaggc agatattttt cttcattgta tgcactgatt acaatggata cgagaagact
     3061 ttcaacattt tgagtcacta ctactctcct gaattttccc ttccaccatt ctatcataat
     3121 tttcaagact tttccatttt tatcttgaaa gccagaaacc ttacttgaca ttatagtgaa
     3181 aaagccttaa aataagctgt tattaaaatc agctagaaga gatattatat gaaaaagcaa
     3241 tcactctttt ttgttccagg tattatcctg attggtgttt ccttgcgaac tccttttact
     3301 gttttaccca ttattttggg aaatatttcg caaggtctgg aggtagaagt tagttcgctt
     3361 ggtgtcttga ccagcctgcc tctccttatg tttaccctct tttcaccatt ttctacccga
     3421 ctggctcaga aaatcggctt ggagcatctc ttcacctaca gcctcttctt cttgaccatc
     3481 ggctcactta ttcgactaat caatctgccc ctgctctatc taggaacctt gatggttggg
     3541 gcaagtgtcg cagtcatcaa tgtcctgctt cctagtctta tccaagccaa tcaaccaaag
     3601 aaaattggtt ttctgaccac cttatatgta acgtctatgg ggattgcaac ggctctggct
     3661 tcctatctag ctgtgcccat tacacaagcc agttcttgga aaggacttat cctcctcctc
     3721 acgttactct gtctagcaac ttttttggtc tggctcccaa atcaccgcta taatcataga
     3781 ctagctccac aaaccaaaca aaaaagtcaa ataaaggtca tgcgtaataa acaggtttgg
     3841 gcaattatta tcttttcagg ttttcaatcc ttgatctttt acaccgccat gacctggtta
     3901 cctaccatgt ctatccatgc aggtctatcc agtcacgaag ctggcttgct gacttctatc
     3961 ttatctctga ttagcattcc tttttcaatg accatcccaa gcctgacaac cagtttatct
     4021 actcgcaacc gtcagctcat gctcactctg gtttcactag ctggtgtggt cggcatttcc
     4081 atgctctttt tcccaatcaa taatttcatt tactggcttg ccatccatct cctcatcgga
     4141 accgcaacca gcgccctctt cccttatctc atggtcaact tttcactcaa gacaagcgcc
     4201 cctgaaaaga cagcccaatt gtccggccta tctcaaacag gaggctatat cctagcagcc
     4261 tttgggccaa ccctctttgg ttacagtttt gacctattcc actcttgggt accagctgta
     4321 gctgccctct tgctcatcga tatcctgatg actgtggccc tctttacagt ggacagagcg
     4381 gataagatcc tttaaacttc tagttttagc tagaagtttt ttatatataa aaattttaca
     4441 catttctctt gacagggctt tcttttagat gtacaatgta tgtgtagaaa aattatatat
     4501 aaaaatctta cacattagaa aaggaggttt cccatgtact ttccaacatc ctctgccttg
     4561 attgaatttc tcatcttggc tgtactggag cagggtgatt cttatggtta tgagattagc
     4621 caaaccatta agctgatcgc taatatcaaa gaatccacac tctatcccat tctcaaaaaa
     4681 ttggaaggca atagctttct gacaacctat tctagagagt tccaaggtcg catgcgcaaa
     4741 tactactcct tgacaaacgg tggtatagag cagctcttga ccctaaaaga tgaatggaca
     4801 ctctatacag acaccatcaa tggcatcata gaagggagta tccgccatga caagaactga
     4861 atacctgact cagctagaac tctatctcaa gaaactacct gaagctgacc gtatcgaagc
     4921 catggactat ttcagagagc tctttgacga tgctggagtc gaaggagaag aagaactcat
     4981 cgctagtttg ggaactccca aagaagtggc ccacgaagtt ctatccaatc ttctcgataa
     5041 aaaaatcaat gaagcacccg ctcaaaaaaa taaccgacaa attttacata tcgccttgtt
     5101 agccctcctt gcagcaccta tcggcattcc tctgggaatc gccatcctcg tgaccctgtt
     5161 cgcaatcctt gtagccgctt tgactgtcat tctggctttc tttgcagttt ccatactggg
     5221 tatcatcggc ggattcctat ttttagttga aagtttcact gtcctcgccc aagccaaatc
     5281 agcctttatc ttgatttttg gttctggttt actggctatc ggtgcttctt cgctagtttt
     5341 acttggcatt tcctatgtag ctcgcttctt cggtctactc attgttcgtc tggtacaatt
     5401 tgttcttaaa aaaggaaaga gaggtaatca gcatgcgtaa atggacaaaa ggatttctca
     5461 tctttggtgt ggtgactacc gttatcggct ttatcctgct ttttgtaggt atccaatctg
     5521 acgggattaa gagcctactt tccatgtcca aagaacctgt ctatgatagc cgtacggaaa
     5581 agctaacctt tggcaaggaa gtcgaaaacc tagaaattac tctccaccaa cacacgctca
     5641 ccatcacaga ctctttcgat gatcaaatcc acatttctta ccatccatct ctttctgctc
     5701 accatgatct tatcaccaat cagaacgata gaactctgag tctcactgat aagaaactgt
     5761 ctgaaactcc gtttctctct tctggaattg gtgggattct tcatatcgca agtagctact
     5821 ctagtcgttt tgaagaagtt attctccgac taccaaaagg gagaactcta aaagggatca
     5881 acatctcagc caatcgcgga caaaccacca tcataaatgc tagccttgaa aatgcgaccc
     5941 tcaatacaaa cagctatatc ctccgaattg aaggaagtcg tatcaaaaac agtaaactca
     6001 caacgcccaa tatcgttaat atctttgata cagttcttac agatagtcag ctagagtcaa
     6061 cagagaatca cttccacgct gaaaatatcc aagtccatgg taaggttgaa ctgactgcca
     6121 aagattatct cagaatcatc ctagaccaga aagaaagcca acgaattaac tgggacatct
     6181 caagcaacta tggttctatc ttccaattca caagagaaaa gcctgaatca agaggtacgg
     6241 aattaagcaa cccttacaaa actgaaaaaa ccgatgtcaa ggatcaactc attgcgagat
     6301 ctgatgataa tattgatcta atatccacac caagcagacg ttgacaaaaa cgcttacatc
     6361 tgctactatg gaagcatatt tatctaacta aaaaggaggt tctaccatga aacaagaatg
     6421 gtttgaaagt aatgattttg taaaaacaac aagcaagaac aagcctgaag agcaagctca
     6481 agaggttgca gacaaggctg aagaaacgat agccgatctc gatacaccaa ttggaaaaaa
     6541 tactcaagta gaggaggaag tctctcaagc tgaagtcgaa ctggaaagcc agcaagaaga
     6601 gaaaattgaa actcctgaag gcggtgaagc gagaacagaa atagaagaaa agaaggcatc
     6661 taattctact gaagaagagc cagacctttc taaagaaaca gaaaaagtca ctatagctga
     6721 agagagccaa gaagctcttc ctcagcaaaa agcaaccacg aaagagccac ttcttatcag
     6781 taaatcttta gaaagtcctt atatccccga ccaagctcca aaatctaggg ataaatggaa
     6841 agagcaagtg cttgattttt ggtcttggct agtggaagcg atcaaatctc ctacaagtaa
     6901 gttggaaaca agtatcacac acagttacac agcctttctc ttgctcattc tgttttctgc
     6961 atcttccttt ttctttagta tctatcacat caaacatgct tactatggac atatagcaag
     7021 cattaacagt cgcttccctg agcagctagc tcctttaact cttttttcta tcatctctat
     7081 cctagtagcg acaacactct tcttcttttc attcctcttg ggtagtttcg ttgtgagacg
     7141 atttatccac caggaaaagg actggacgct agacaaggtt ctccaacaat atagtcaact
     7201 cttggcaatt ccaatcttcc tcactgctat tgctagtttc tttgctttct ttgatagcct
     7261 acgatttacg gccctcttgt gtgtgattag cattggaatc attctgcttg ccagtctcca
     7321 tatcattaca agaccaagtc aagcaagtga aaccgactcc ttctatcaat tattcttgtc
     7381 tgtccttgtg aacggagtta ttatcctcct cttctttgta gctgaagtgg cactgattgg
     7441 agattatctt cgtatcttgg cctttcttta aaattcaatc ctgtcatcct tggcaggatt
     7501 ttttctatgt caaaaaagca agtcgattga cctgcttctg ctttactttt cttgtgctaa
     7561 tgttttaaaa tctttgtcta atatgggttg cacttctatt tgattggcgt ctagttggtg
     7621 gtaaaaagat gggggtaata cctctaagaa ttttgcatag tccaagcgag caatggcttc
     7681 atagtcttct ggtttagagc catctcctgt gatttgaacc aagtcaatct cccactgcac
     7741 ttccttatcc accaattgct caatataggc tgcaggaaca aacggttctc tcggtaaaac
     7801 tgtcttgact ccttgagcta gatggtaaac gccatgcact tttccttctc catcctgata
     7861 gaaggaaact ctcgcaaagt aggcatctgt ctcttgaacc gcacgcacac gcgcttcaaa
     7921 ctgagccaaa ccatctgcct ctaaaaagct tttcaagttc tcacgactaa agaaaatagg
     7981 attaaaaatt ccttggcaaa tcgtaaaacg agctacaggg tctgctagat aggcttgaat
     8041 acttgcttgg aaataagctt caaaatcaaa accatctagc ccttcaatat cagtccctgt
     8101 ataagcaagc aaagcatcta aaaatgactt gataatctgc cagtctgtct ctgttagtag
     8161 gtcaggaaga tcgacacggt aagccttttg accatcagca taactgacct taaaaagaaa
     8221 ttgtgattgc cccactatcg cacactcaat atactcgaga cgattaagag gctgacggag
     8281 atagactaca tcatagatat gtgactccaa accgtctagc aatgccaaga tcgacttggc
     8341 attcaaaatc tcttgttctc ctaaaatgct ctgtttattt ggaataaaaa atgttttcgc
     8401 cataactgga ctcctttgaa atcgcttaca tacagtatac cttatttttc tgaaagatac
     8461 agaaacaaac tttagatttt tgagtacaaa gcatagaaaa acagcccctg aggactgttt
     8521 gatcttatac agtagctgct tgatccaagc tttcaccgat agcggctagg cgctcgatca
     8581 cttcagcttg tgtcaattca ttttttgaaa catagcggtt acgtgggtga acacggcact
     8641 cgtgtgagca tccacgaagg tacttgtctt cattttcttc tgatgtcaag atacgacggt
     8701 tacagaaggg atttccacag ttgacataac gttcacatgg tgttccatca aaccagtctt
     8761 tccctacgat ggttgggttg acatggttga catcaacggc gatacgctcg tcaaatacgt
     8821 acatttttcc atcccaaagc tcaccttgaa cttctgggtc tttaccgtaa gttgcgattc
     8881 ctccgtgcaa ttggccgaca tctttgtagc cttcacggac catccagcct gagaatttct
     8941 cacagcgaac gccacctgta cagtaaacca cgacacgctt gtccatgaat ttttccttgt
     9001 tatcacggac ccattgtggt aactcacgga agttgcgaat atctgggcga atagctccac
     9061 ggaaatgtcc taggtcgtac tcataatcgt tacgtgtgtc aaggacaacg gtatctttat
     9121 caagaagcgc ttctttgaac tcttttggag acaagtaagc acctgttgtt tcaagtgggt
     9181 tgatgtcatt gtcaaagtcg ttgtcttcca aaccaaggtg gacaatttct ttcttgtagc
     9241 gaacaaacat cttcttgaag gcttgttcat tttcttcgtc aatcttgaac cagagttctt
     9301 ccattcctgg aaggctgtga acgtagtcca tgtatttttg agttgtttca tagtcacctg
     9361 aaactgttcc gttaattccc tcgtcagcga ctaggatacg gcctttaaga ccgattgatt
     9421 tacagaaagc caagtggtct gcagcaaatt gctctgcatt ttcaattgga gtataaaggt
     9481 agtaaagtaa gacacgaata tcttttgcca taagatttgt tctcttttct attcttaaat
     9541 tttctaaatt ttttatttat ccatactagt atacctgctt gagaaaacga atgcaattta
     9601 tttgactgga aattgtagag gattctcgaa atttcctgaa tggttaaaat aaggacaaga
     9661 gaaaacatgg atatctatat ccttgtgcca aaaaaaccac tgccctcccc agaccaacct
     9721 gaggaaagca gtgattctta ttttaggagt taggaatgaa tacacgaaat caatttagct
     9781 gattattttt tgtttttcaa gaattcatcg tattgttttt gcatttcgtt caatactttt
     9841 tcgtaggcac cttcagattt caatttttcc atcaattctg gaatcgcttt atctgggtct
     9901 acagtaccag tgttgatagc tgtatcaaat tgttgcattg tgttagcaat agctgagatt
     9961 tcagatttca cattgtcagt attgaagata aatccaagcg ctggagattc tttagcttct
    10021 gccaattctt tcttagaatt ttcgatttgt tggtctgtaa cgttttcgtt gatgtaaagg
    10081 atccagttgt taccagtgtt ccatccaccc atgtgagtgt ttcctttgta gccatcaaga
    10141 acgcgaacac ggttttcttt accttcaatt ttttcccagt tcttgccttc tggaccgtaa
    10201 acaagaccgt tcaaaagttc tgggttcgta ttcaagaggt tcaagatttc cattgatttt
    10261 tctttgttct tagagttgtt tgagatgaca aagttagcaa cttgtgttgt ttggtttttc
    10321 ttgatgaagt tagtgattgg tttgatttgg atatctttgt tggcaacacg tgaaagcaag
    10381 ctgttaccgt agtcagctgg tcctactgtt tcttcacgaa cgaaccaagt atcttgttga
    10441 aggtcaaatg aagtatcgct tgttgcgacg tcttttggaa tgtagccagc ttcatagaat
    10501 ttgtgaagag tcttcaagtg ttctttgaaa cgaggcactt cgtaacggtt tacaacttta
    10561 gtagtatcgc cttcaaggtc aataacgaat ggaagcccgt ttgctactgg gtagtcaaaa
    10621 ttatcagatg ggatgaaaac tttaccaata gcaaatggta ctacgtctgg agctttttct
    10681 ttgatttgtt tcaagactgg ctcaagagtt tcgtaagaag taacacctga aatatcgata
    10741 ccatatttgg caaggagagt tccgttgaag gcaaagtttt gagatgatgc tacgttggct
    10801 gcaactggta cagcataaat cttaccattt acagtattac ccttgatata agctggatca
    10861 agtgccttgt aaaggtcttt accttctttt ttgtacaatt ctgtcaagtc agcataagca
    10921 cctttttgag catttacaat atagttatct gcaaaggcga tatcatagtt ttcaccagat
    10981 gatgtgataa ctgacatttt cttaccatag tcaccccagc caaggtattg gatatccaat
    11041 ttggcaccaa ctttttcttc gatgattttg ttggcatttg ctaacaattc atccaagtta
    11101 tctggtttgt caccgatttg gtacattttg ataacaggtt tgtcacctga atcagcagct
    11161 tttttgctgt tacctgtcaa atttccacaa gcagcaagac ctgcagccag agcgactaca
    11221 ctagcagatg caaaagcata ttttttccag tttttcatga taaaaactcc tttttttatt
    11281 tttaaactta taaacaatgt aatgatctta acttcacaca agggaagttg ggaactgaga
    11341 aatgttcttt ttcaataagc actattcttt cacaccaccg atagtcaaac cttttacaaa
    11401 gtagcgttgg aagaatggat acaaaatcgc gattggaagg gttgctacca caaccatggc
    11461 catacgacct gtttctttcg gtagagcaac tcccagttga ccagtcaagc cgaccgcttt
    11521 ggcaatgtag tccatatttt gttggatttg catgagcaaa tattgcaatg gatacaagtt
    11581 atcactcttg atgtaaagaa gggcgttgaa ccagtcattc cagaaaccaa gagctgtcaa
    11641 aagcgtgatg gttgcgatac ctggtagtga caatggcaaa cagatttgga agaaaatccg
    11701 agcctcactg gcaccatcga tacgagccga ttcgagaatg gcttctggaa tggtcttctt
    11761 gaaaaaagaa cgcatcaaga tgatattgaa tggtgagaga agcattggaa caatcaaggc
    11821 ccaaacagtg tcaccaagct gaagtacacg ggtcaccatg atataacctg gtaccaaacc
    11881 agcgttgaac aacatactga gaaggacgaa gatggtaaaa aatctgcgat acttaaaggt
    11941 tgtccgtgaa atagcgtagg cataggttgt tgtgataaag acatttgtca atgtcccaac
    12001 tacggttaca aagacagaga tgaagagggc ttgtaggatt ttatccttaa actgtgccaa
    12061 aaactcaaaa ccgtctaagc caaattggga tgggaagaag ctatagccgt attggaggag
    12121 gcttttctcg tctgtcactg aaataatgat aacgaataca aaaggtagga tacaagagag
    12181 ggcaatcaaa cccgaaatga tactgaagaa gatatctgct ttcttactga aggagtgaat
    12241 gccgacatta tcaatttttt cttttttaat tttctttttt gccatattct cctcctttct
    12301 agaacaaagc tgagtttgga tcgactcgtc ttgcaagcaa gtttgatagg ataaccagaa
    12361 tcaaaccaac aacggattgg taaagaccgg ctgctgcagc cataccgata tctgctgtct
    12421 gagtcaaacc attaaagaca tatacgtcca aaacgttggt tacattgtaa agctgaccag
    12481 cattgtgtgg gatttgatag aagagaccga agtctgcgcg gaagatattt ccgactgcaa
    12541 ggatggtcaa tacagttaca agcggagtca actgaggaat ggttacgttg cgaatacgtt
    12601 gccacttgct agctccgtcc actgtcgctg cttcgtagta ggttggatca attcccatga
    12661 tcgtcgcata gtacatgaca ctgctatatc caaagccttt ccaaatacct aggaaaagta
    12721 ggagataggg ccagatgccc aggtcagcgt agaaattgac ttctttgaga ccaagacttt
    12781 ccaatagatg attgaacacc cctttatcaa tatttaggaa ggcatctgta aagaaactga
    12841 tgataaccca agacaagaag taagggaaca acatagaagt ttgaaaaatc ttcaccattc
    12901 tcttagaacg gagctcgctg aggataatgg caatccctac agatacaact aaacctagaa
    12961 agataaagcc aagattgtag aggacagtat ttcgtgtgat aataaaggcg tctcttgaac
    13021 taaataagaa tctgaaatta tcgagtccga cccatttact atttatgata ctatctatga
    13081 aaccattact ggtcatgtgg tagtctttga aggcaaccac gttcccaaat actggaatgt
    13141 aaaagaatag aatcaaccag agtgcccctg gcaaaaccat caagagaaag atccagttgt
    13201 ctctcaatgt ttttgaaaac tttttcataa tttcctccct ttttattttg atatccatct
    13261 aaaaattctt ttttagactt ttgataacga ttacattatt agtatactcc tatttgcagg
    13321 ttaggttaaa ctcctaatta tagaaaaaac tccacaaatt atgtagcaga tttaaaactt
    13381 tatcaccact atcaaacaaa tgtcctaaat caattgttta ttttatctct attagcccag
    13441 tgatggcgtc actctgttat aagcatccaa caacggggta tactgaaaaa tctccagact
    13501 agggaactca gcgatagttc ctaatctgga gatttttaat atgttattag gcgtttgctt
    13561 tcaacttagc aataacctct ttaagattat caatcaactc tgcttcagta tgctcagagc
    13621 ctttttcatc tgccaagaac aaaactgctt tttgaagttc ttcttgagag ttttcaagga
    13681 catccttatc tgctgtttct ttgcccgctg tttgtcctcc atccttagtt gctgactggc
    13741 tttccttaat ggactctagg gaagcaatgg catctttgac tgtttgcaag atatcacgta
    13801 aaccttgctc tgtcaaacta tcatctgcaa aagctttctt agcctctgcc aaaaccagac
    13861 gtgctgaatc tgtggtagga ttcgatacac ctgtcaatga tctcaaaaga ttttctaaag
    13921 tttgagtctg cttactaata ctagactaaa atcaaaaagc attatataac agtgatatga
    13981 aatcaactaa agaagaaatc caaactatca aaacactttt aaaagactct cgtacagcta
    14041 aatatcataa acgccttcaa atcgttctat agtaaaatga aacaagaaca ggacaaatcg
    14101 atcaggacag tcaaatcgat ttctaacaat gttttagaag tagaggtgta ctattctagt
    14161 ttcaatccat tatatttcgt ctgatgggca aatcttataa agagattata gaacttttat
    14221 agtagtttga aataagatgt gaacaactct atcaggaaag tcaaattaat ttatagaaat
    14281 attttagcag ctaaggtgta ctgttataga ttcaatacac tatacttggt ggtttagctc
    14341 tcctgttttc tcttttagct ttaaccagcc ataaatggta ttacgtgaga tttggaaaac
    14401 gtgtgatgct tctgttatac tacctgttcg ctcacaataa gagagaattt ttttacgaaa
    14461 atctattgaa tatgccataa aaggattata ccacattgtg tactattttt ggttcatttt
    14521 actatatcac tttggactat atcggtctag ttatgtctaa acgattatag ctggatacta
    14581 agagaaatca aaaaagcact aagggagaac gtcccaaagt gcttaatatc aaggcctcaa
    14641 agcctgtctt attcaataaa atattacaac atcttgttgt agaattcaac gacaagtgct
    14701 tcgttgattt ctgggttgat ttcgtcgcgt tctggcaagc gagtcaatga accttccaat
    14761 ttttcagcgt cgaatgatac gaatgctgga cgtccaagag tagcttctac tgcttcaagg
    14821 attgctggaa ctttcaatga tttttcacga actgagatca cttgacctgg agttacgcgg
    14881 tatgatggga tatcaacgcg tttcccgtca acaaggatgt gaccgtggtt tacgaattga
    14941 cgagcttgac gacgagtagt cgcgagacca agacggtaaa caacgttatc caaacgacgt
    15001 tccaaaagaa gcataaagtt gaaacctagg attccgcctt tgatttttgt agcttgtacg
    15061 aacaagttac ggaattgttt ttcacctaca ccgtaagtga aacgaagttt ttgtttttca
    15121 gccaattgca aaccgtattc tgacaattta gaacggttgt ttggtccgtg ttgtcctggt
    15181 acgtagttac gacgtgccaa ttctttacct gtacctgtaa gtgaaaggcc aaggcgacga
    15241 gcttgtttcc aagatggtcc tgtataacgt gacatatgaa tgtcctcctg atataaataa
    15301 tatttcggtg gaaatagtca cttagaaagc cctgattcgt gcagatgccc ttcgcctaaa
    15361 cagccaaggt tacttgtcat aagacacctg ttgacgagct tcatgctttc ctgctgatat
    15421 ttcacacaaa atccatttta ccatgaatag ctggatttgt aaaggggttt taggctttat
    15481 tttcactacc agagagattg agaacgaggg taaaggtact tcctagaccg tactggctgc
    15541 tgactgtgat ttccccaccc aattgatggg ccaattcacg cgcaatcgca agtcctaatc
    15601 catgaccacc tgtcttcatg ttacgcgaag tttcgacacg ataaaggcgt ttgaaaatat
    15661 tttccaaatc ctctggggca atgccctgcc cttcatcggt cacactgatt gaaagctggc
    15721 ccttctccag cttagtcacc acttccagct tggttcctgg agcagaatat ttaaaagcgt
    15781 tatcgaccag attcaccaag atacgagaaa gcttagcata atctccctca atccgggcag
    15841 actctgggat tacctgcaag tggacatctc ttctctcctg ctcaatcaaa aactgaaatt
    15901 cactcatgca ctcaattaag agcttgtcca gaaaaatact gtctttactg gtagtttcca
    15961 cctgatttct agctgtgttt agggtcaaaa aattcaactc ctcaaccagt ttattgagcc
    16021 tctccgtctg gcgtccaatg gttgctagat aatgagcttg ctccgactcc ttgataatcc
    16081 catccaaaat cccttctacc gtcgcttgga tcgaagtgat aggggtctta atatcatgcg
    16141 acaactgggc aatcatcaag cccttttctc gttcgctttc ttccaaggaa tcaaagctta
    16201 cctgcaaatc atgggacatc tcattaaaag tttgccctaa ttgctgaaat tctacaggac
    16261 cttgaacctc caaatttgaa ggaaaatcct tggccgctac ccgcttggca tgctccttga
    16321 gtttgcccaa cgacgtaaag actggcaata ggagaaagag actaatccca gcaccgacaa
    16381 ggctggcaac gatggtcatc ccaagcaaaa agtaaatctc gcctttcgca atcagcattt
    16441 tttgaacagc ccaaaaaaca accaaaatgg ttaagagggt tgaaataata tatccaacca
    16501 aaatataact ttttagtttc atgtttgtcc tctcggtttc tctatcttat atcccaaccc
    16561 ccaaactgtc ttaatagtgg gcgtttggtc actactatat tttgccagct cctgtcgaag
    16621 agcatggata tgcacattca aggtattggt gtcatccacg tagtcttctt tccagatctt
    16681 ttcatagagg tctgtcttgg agaaaactcg ctctggattg ctagctaaaa tccacagcaa
    16741 ttcaaatgat ttaacagtta aatccagcat ttcttctcct atttgaactt catgactact
    16801 atgattcatt tttagattgc caagggaaat cagctctgtt tcgcccccac gatgaaggcg
    16861 gcgcaaaata ttgtggacac gcaaaaccag ctcacgtggg ctaaaaggct tagcaataaa
    16921 atcatctgct cccaagctca ggccgtaaat cttgtcctgt tcactggtct tagcagtaat
    16981 aaataggaaa ggctgctctg gtgataagta ttgaacctca ctgattaaat cataaccatc
    17041 catccgaggc atcataacat ctgtgataat caaatcaatc ggttttttct tgaacagctc
    17101 tagcgcttcc agtccatcat gggctaccaa gacctgataa cctgcctgaa ttaagtatct
    17161 ctgatgaata tctgtgattt ctacctcgtc gtcaacgagt aaaattgtct ttcccatctg
    17221 tctctccttt gataaaaaca gtgctatact gctttaagta taacactatt tttattgttt
    17281 tacgatgatt ttaatctgct attgaaagaa agtcctgatt gctgattgag ttgttaaact
    17341 aatcttagtc agcccactat ccatcattta tttccttagc tgattagttt tcttcttttt
    17401 tgtttttcaa acctagacca ccgagtaaac ctgcaagcgc aagcccaagg aaaccaatac
    17461 ttgccattga tgtttgagtc tcaccagtat ttggtagcat agctttatcc tctgacatca
    17521 tcgtatcaga catcttgtta gcagaagcag ccatgttttc acctgccatc gtgttggtag
    17581 aacttgtcat ggtgtcagca ggcatgctat ctgtagtacc tgtagcatga ttgtgattca
    17641 tcggagtcac gccagaacca gagttagaag gtgataatga accatttgct gtgtctgatg
    17701 tttctttaac
//
LOCUS       DAVBCN010000042        17251 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVBCN010000042 DAVBCN010000000
VERSION     DAVBCN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17251
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.042"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(<1..692)
                     /locus_tag="VQ497_001290"
     CDS             complement(<1..692)
                     /locus_tag="VQ497_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HET2517594.1"
                     /translation="MNIILIAKPLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSYRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDG"
     gene            complement(803..1057)
                     /locus_tag="VQ497_001291"
     CDS             complement(803..1057)
                     /locus_tag="VQ497_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517595.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQV
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            complement(1415..1948)
                     /locus_tag="VQ497_001292"
     CDS             complement(1415..1948)
                     /locus_tag="VQ497_001292"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET2517596.1"
                     /translation="MTSFKEQEPEKVAEFLDILDNLKDLPVLYIDETGIDRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVMDLL
                     REVPTIFECLECFFKTR"
     gene            complement(1935..2150)
                     /locus_tag="VQ497_001293"
     CDS             complement(1935..2150)
                     /locus_tag="VQ497_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2517597.1"
                     /translation="MGKERREQGHIERKKRVVKNRKIPLEELKAFVEAHPDAFLREIA
                     AHFDCAVPSVWAALKQIKVTLKKDDEL"
     gene            complement(2117..2260)
                     /locus_tag="VQ497_001294"
     CDS             complement(2117..2260)
                     /locus_tag="VQ497_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HET2517598.1"
                     /translation="MAYSTDFKQRALDYIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KDT"
     gene            2494..4212
                     /locus_tag="VQ497_001295"
     CDS             2494..4212
                     /locus_tag="VQ497_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HET2517599.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            4323..4670
                     /locus_tag="VQ497_001296"
     CDS             4323..4670
                     /locus_tag="VQ497_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET2517600.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFIHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(4949..5785)
                     /locus_tag="VQ497_001297"
     CDS             complement(4949..5785)
                     /locus_tag="VQ497_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2517601.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPELLKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(5798..6427)
                     /locus_tag="VQ497_001298"
     CDS             complement(5798..6427)
                     /locus_tag="VQ497_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2517602.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLDNLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(6437..7078)
                     /locus_tag="VQ497_001299"
     CDS             complement(6437..7078)
                     /locus_tag="VQ497_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2517603.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(7224..8453)
                     /locus_tag="VQ497_001300"
     CDS             complement(7224..8453)
                     /locus_tag="VQ497_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET2517604.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(8443..9609)
                     /locus_tag="VQ497_001301"
     CDS             complement(8443..9609)
                     /locus_tag="VQ497_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET2517605.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            9753..10766
                     /locus_tag="VQ497_001302"
     CDS             9753..10766
                     /locus_tag="VQ497_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HET2517606.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FVRMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(10811..11230)
                     /locus_tag="VQ497_001303"
     CDS             complement(10811..11230)
                     /locus_tag="VQ497_001303"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET2517607.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(11241..12647)
                     /gene="atpD"
                     /locus_tag="VQ497_001304"
     CDS             complement(11241..12647)
                     /gene="atpD"
                     /locus_tag="VQ497_001304"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET2517608.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMEF"
     gene            complement(12733..13611)
                     /locus_tag="VQ497_001305"
     CDS             complement(12733..13611)
                     /locus_tag="VQ497_001305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET2517609.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(13627..15132)
                     /locus_tag="VQ497_001306"
     CDS             complement(13627..15132)
                     /locus_tag="VQ497_001306"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET2517610.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQSVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(15147..15683)
                     /locus_tag="VQ497_001307"
     CDS             complement(15147..15683)
                     /locus_tag="VQ497_001307"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HET2517611.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(15683..16177)
                     /gene="atpF"
                     /locus_tag="VQ497_001308"
     CDS             complement(15683..16177)
                     /gene="atpF"
                     /locus_tag="VQ497_001308"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET2517612.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(16191..16907)
                     /gene="atpB"
                     /locus_tag="VQ497_001309"
     CDS             complement(16191..16907)
                     /gene="atpB"
                     /locus_tag="VQ497_001309"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET2517613.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(16942..17142)
                     /locus_tag="VQ497_001310"
     CDS             complement(16942..17142)
                     /locus_tag="VQ497_001310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HET2517614.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
BASE COUNT         5209 a         3781 c         3095 g         5166 t
ORIGIN      
        1 ccgtcagtag tagtcatgct actgagtttt ttatctgtga cattgtagct ggtaccaatc
       61 agttgttttt tattggtaat gtaggttccg tcactttctt tttctccaat tccagtatca
      121 tttttcaatg atagcaaacg cccttgttca tcaacatagc gaactttcac attttctgag
      181 attagttctg ccaattctga ggtttttttc tttttcttga tttcttcggt tattttccct
      241 ttctcttctt cgggaatatt tagttttgga atgatttttt caacaacggt tcgtgatggt
      301 tccacagtat cttggatgac tgaaaagtca gctagaattg ggagattata ctgaacacgg
      361 taactttgag tgtttactcc tactctttca ttattctctg aaaatacttg tacggtataa
      421 gaaacaacat cttttcctaa tagaacatcc ccagtagaga aatagccgcc ttttcctagt
      481 ttgctatctc cagagcccac ttctttccta attttatcag atagtttttt accagtcagt
      541 acattcgttc gcacaatccc tttgtctacc cctacaaagt gggagaactt tttgaactct
      601 tcagaaccag atctagccca accattatta agggcatttg cttttgtatt tgtattctct
      661 ctcaaaggtt tggcgattag aattatattc atcggcactt agagttgctg ctatatctga
      721 ctcttgaata ccaacttcct tactaccatt tctagcggca gtatatgtgt atttatctgt
      781 ttctacttct agctgattat ccttaacatg ataggtaatt ttctcctggt ataagagtct
      841 tcagtagttt atatactctg tcatcatctt tagctttaga agcagctgag tttagccaag
      901 ttggggtatt aggagtatct ttatcaacct gtttcaagac attattcaag tcatttgcta
      961 gcctattggt tatcctcact gtgaaagttt tcttccagtc tgaaccactt ttattagact
     1021 tagatatgct catgaaatcc tggtcaacat tcaacacaat atcgccttgt tttttagcat
     1081 cttccgttgc ttctgcttca aattgatcat ggagtttttg ttgctctggt gtatagtttt
     1141 tttatttaca cgatttaatg tactattttt tattttagta ttaagaactc cattaatacg
     1201 atttttaaac tctgcttcaa aaaatccctt aatttgttgg tgcgcaagtt caacattcaa
     1261 acctttctta tacaagtctt cattctgata caagtcttca ttctgaacga tttcggctac
     1321 acggtgaatg gtttgatcgt ataccgtctt ctcttaacca gcagtatcct tagttttaat
     1381 gactactcga taggttcctt tagaaccgtt atagtcatct agttttaaaa aagcattcca
     1441 aacattcaaa aatagttgga acttccctta ataaatccat cactttcttt ttcaagatag
     1501 cccaagcttg ctcaatagga ttcaaatccg gtgaataagg tggtagaggt aagaaaaagt
     1561 gtttatcttg gatgcagagt tcatccaaaa tgttcttggg atgaaaacta gcattgccca
     1621 tgacaataac atgaggtgtc ttcaaagcag gtaggagttg cgttttgaac cactccacaa
     1681 agaaatcgct tgtcatgctt ttcttgtaaa tcatgggagc tataaactct ccgtctactt
     1741 gtcctgcaac aattgaagtt cgctcaaaac gacgtccgct aatcttttca tagactttct
     1801 cccctctagg agcccctgca taaggacgat agaggtagcg gtcgattcct gtttcatcaa
     1861 tatataggac tggtaaatct tttaggttat ccaaaatatc aagaaactca gcgactttct
     1921 ctggctcttg ttccttaaag ctcgtcatct ttttttaaag tgaccttaat ctgctttaaa
     1981 gctgcccata ctgaaggaac agcacaatca aaatgtgccg caatttcccg taaaaaagca
     2041 tctggatgag cctctacaaa ggctttcaat tcctctaaag gaatctttcg gtttttgacg
     2101 actcgctttt tcctctctat gtgtccttgt tcacgtcttt cttttcccac gtgaagagag
     2161 ttctgacgcc aacaccaaaa aactttgctg cctcgacatg gctgtgcccc tctttgatgt
     2221 aatctaatgc tcgctgttta aaatcagtac tatatgccat agctttatta taatatgctt
     2281 gtttaaaact aagcaactat atcaacgttt tgttatgaga agacttactt tttttactga
     2341 aattgaattt ttcctagcct caacatcata taaccccaaa attcaatctg acctggtttg
     2401 aagagatttt cgaagcagat aaggttttac taaaactaaa aaaaatgata taatcaaact
     2461 gattaaatgt tttaaaacta aaggagaatt tgaatgtctt accaagaaaa ttaccagaaa
     2521 tgggttgatt ttgtagagct tcctgactac cttcgtcaag atttggaaaa tatggacgaa
     2581 aaaactaagg aagatgcctt ctatacaaat cttgaatttg gtactgcagg tatgcgtggc
     2641 ttggttggtg ctggtacaaa ccgtatcaac atctacgttg ttcgccaagc tactgaagga
     2701 ttggctcgtt tgattgagtc aaaaggtgga aacgagaaag aacgcggtgt agcaattgcc
     2761 tacgatagcc gtcacttctc acctgagttt gcctttgaat ctgcggcagt tcttgctaaa
     2821 cacggcatca aatcttacgt atttgaaagc cttcgtccaa ctccagaact atcatttgca
     2881 gttcgtcatc tcaactgttt cgcaggtatc atggtcacag ccagccacaa ccctgctcca
     2941 tttaacggtt acaaggttta cggtgaagac ggtggacaaa tgcctccaca cgatgcggac
     3001 gctttgacta cttatatccg tgcaatcgaa aacccatttg cagttgaagt tgctgatgtg
     3061 gaaactgaaa aagcttctgg cttgattgaa gttatcggcg aagctgttga cgtagaatac
     3121 cttaaagagg ttaaggacgt aaacatcaac ccagccttga ttgaagaatt tggtaaagac
     3181 atgaagattg tctacacacc acttcatggt actggtgaaa tgttggctcg tcgtgctctt
     3241 gcccaagcag gatttgactc tgttcaagtt gttgaagcgc aagcaactgc tgacccagac
     3301 ttctcaactg taacatctcc aaacccagaa agccaagcag cctttgccct tgctgaagaa
     3361 cttggtcgtc aagttggtgc agatgttctt gtcgcaactg acccagacgc tgaccgtgtt
     3421 ggtgttgaag ttcttcaaaa agatggtagc tacctcaacc tttcaggtaa ccaaatcggt
     3481 gctatcatgg ctaaatacat cttggaagct cacaaaaacg ctggaactct tcctgaaaat
     3541 gccgctctct gcaaatctat cgtttcaact gacttggtaa cgaagattgc tgaaagctac
     3601 ggcgcaacta tgttcaacgt cttgacaggt ttcaaattta tcgctgaaaa aatccaagaa
     3661 ttcgaagaaa aacacaacca cacttacatg atgggatttg aagaaagctt cggttacttg
     3721 attaaaccat ttgtacgtga taaagatgcc atccaagccg ttcttgtcgt tgctgaactt
     3781 gctgcctact accgttctcg tggtttgaca cttgctgacg gtatcgaaga aatctataaa
     3841 gaatatggct actacgcaga aaaaacaatc tctgttactc tttcaggtgt cgatggtgct
     3901 gaacaaatca aagcgattat ggctaaattc cgcaacaatg ctccaacaga atggaacgca
     3961 acagctatca ctgtcgtaga agacttcaag gcacaaactg ctactgttgc tgacggtact
     4021 gttacaaact tgacaactcc tccaagtgat gtgttgaaat acacacttgc tgacggttca
     4081 tggattgccg ttcgcccttc aggtacagaa ccaaaaatca agttctacat tgcagttgta
     4141 ggggaaacca acgaagaatc acaagctaag attgctaaca tcgaagcaga aatcaatgca
     4201 tttgtaaaat aaagacctat aaaagatgag accaaccaac ctcatctttt ttcgtatcaa
     4261 atctaagcaa ttttagaaac tttatggcat actagactta tcaatgaaag cgaggtaatc
     4321 tcatggaaca atttttagat aacatcaaag accttgaagt cactacagtt gtgcgtgcgc
     4381 aagaagctct tgataaaaaa gaaactgcaa ccttctttat cggtcgcaaa acttgccctt
     4441 actgccgtaa atttgcaggt acattgtcag gtgtcgtagc tgaaaccaaa gctcacattt
     4501 acttcatcaa tagtgaagaa ccaagccaac tcaatgattt gcaagcattc cgctcacgtt
     4561 atggaatccc aactgtacca ggttttattc atattacaga tggacaaatc aacgtccgtt
     4621 gcgactcttc aatgtcagca caagaaatca aagatttcgc aggattgtaa aaaatcagct
     4681 aacaagtctt aaaatcacaa aaacgcataa tgtaaggtgc aaaaaccttg atattatgcg
     4741 ttttattgtt cctaaaacag ttacatcagc atttcattac agaacatttt aattttggaa
     4801 gtcaaatcaa tttttaacaa tgttttagaa atcacagtgg gctattccat attcaattat
     4861 ccagattcaa tcgctattat cgttttccta gattacaaaa aaaactacgt tttgctaatc
     4921 aaaacgtagt ctcagccact attttatctt actgtccttc ttttacttct ttggttgcta
     4981 catcttctcc aaaccatttt tggctgattt cttggaactt gccgtccttg taaagactag
     5041 aaaaagcttc atttatcttc ttaaccaagg ttgtatcttc cttacgggct ccaaccgcaa
     5101 aagcttctgt ttctagtcca actgtaaaga cattataatc gtttaaaaca ccttctgctt
     5161 ctaaataata gtttgcatag acacggtcaa tcaatagacc atcaattcga tcgtttttca
     5221 aatcaatcaa ggcttcatta aaggtttggt attgattcgc ttccttatta gcgacaatat
     5281 tcttcaaaag ttctggattt gcttcaaagt ccgcataacc agatgaacca gcttgagctc
     5341 ctaatgtctt tccagtcata tcctttgcag tcgtgatacc agatgatttc ttcgtaacca
     5401 atacctgctc attcttcata tatgagttac tgaaagccac cttttcacgg cgttcgtctg
     5461 tagcggaata gccattccaa atcagatcaa tcgttccttt tgtcaattca gcttctttca
     5521 aatcccaatc aatcggttgc caatttaccg tgattccgta tttttcaaaa acagctgtag
     5581 ctaaatcaat atcaaatcct gcataagaac catctttctg agcaaatccc attggaacaa
     5641 aagtactatc aaatccaata gtaatagact tgttagactg gtactttgac caattatctc
     5701 cactagtttc gctagaattt ttcccacaag ctactaagaa caaagcagtc atcagactga
     5761 ctaatacaag catccatttt ttcatccatt tttcctccta tttaggttct actttcaata
     5821 atacatctgc gatattttca gcaaactgca aatcatgggt aaccacaatc tgggtcatcc
     5881 caagttccct attttgcaag attagcttct ccacttccaa acgtaattct ggatccaggg
     5941 cagaagttgg ttcatcgtag ccaatgattt ctgggtcaat catcatagca cgcgccaaag
     6001 ccacccgctg cttttgccca ccagatagtg agaaaggata ggcctctgcg tgtcctccta
     6061 gtcctaactg ttccaagagt ccactcgcct tcttctcagc ctcttcctgc ttcattccca
     6121 tggtcttcac aggcgataag gtcaaattgt ccagaactga taaatgaggg aacagttgaa
     6181 aatcttggaa gacaaatccc agtagattgc gcttctgcaa ttcatccagc tctaaaggtt
     6241 gtccattata aaagatttgc cctgaatcaa tggtttcaag acctgcaagc atacgtaaaa
     6301 gagttgtctt acctccacca gaaggtccaa cgatagccag gatttgcttt tcaggaatac
     6361 ttagactgaa attagacagg atttgtttgt ctccaaagac tttattgata tttcgtaatt
     6421 ctaacatggc agcctcctat ctataataac tatacttctt ctcaactttt ttggaaataa
     6481 ttgtcacaat cccaatcaaa atcaaataaa tggctcctgc caagaacata ggaactagac
     6541 tagcatcgcg gttagcagct gttcgactag ccaagataag atctgaaatt ccgagagcat
     6601 agaccaaaga agtatccttg accaaactca taacttcatt aaagacacta ggaagaacga
     6661 tcttggtcac ttggggcaag ataatatagc gcactctgtc aaaagggcta aacttcaaga
     6721 ccttggcggc ctcatactgt cctcttggaa tagtgtcaat tcccccacgg aaaatttctg
     6781 caaagtaagc tgcatagttg agaacaaagg caataatagc tgcaggaagg cggtctaaac
     6841 gaatcccaat acttgggagc acataataga taaaaatcag ttgcaagagt aagggggttc
     6901 ctcgcataac ccagatgtaa atgttaatca gataatggag gggcttccaa tggacttgca
     6961 aggcaaaggc aatcaaaacg cccaacggaa tcgaaaatag caagaccagt gcaaagacct
     7021 gtacagtcgt gctcgcacca ttcagtaaac tcggtaatat ctcaaataaa taagacatac
     7081 tgcacctcct aaaagtaatt gttcctatta tagcatgaat aaacaagatt accaagtatt
     7141 tttgtaaaaa gattctacca tatacaaata aaaaccgtag ctatcaaaag agataaacta
     7201 cagttctata tgtttaaaca ttcttacaat ctctcaaaca gttcttgcat ttgcacatca
     7261 tctggaacca gttttaagta agtgtgagca tggacttttg cttcttcaaa atgtcccaat
     7321 tcacgcaaga gatagatata gtgttccaga aattctggat tgtccttcaa atctcctgtc
     7381 aactcttgat aatgctcata agcagtatcc aaatcgtcca tttcttgata agaacgagca
     7441 atcatccact tggtcaaaag attttctggc tcctcactct gcaagtctag aatatcctca
     7501 taacgctcct gctccagata aatagtggct aaacgaagca agatttcttc tgtatcctca
     7561 gcgtcttctt ttgcagtaag gagataattt tctgcaccac tagcatcatg caattcataa
     7621 gaaaattgtg aagcagctag caagaggcga gtttcaaagg gatttttctc taatccttgc
     7681 ttagcgatac gcagggcttc ttgaacttga tgttccttat gtaaagcctg actgtaccca
     7741 tactcatagc cttcaaagtc aggagaaatg gtatcaagct gcttaaagta gagggtggct
     7801 ttttgatatt cttcttgatc aaaataaaga ctggccaact caaaagctgt taagtcatcg
     7861 tattctaact ccagggcttt ttctaaaaac tcagtagccg tttcaaattt ccctaactga
     7921 gcataggcaa agccaattcg ttgataggtg gaaatgcccg tttgctcata aatcgagcga
     7981 ttatctaact gggcataggc ttgaatagcc gcttggtaat tttccaactc actatccaac
     8041 tctgccaaac ccaatatcaa gagagaatcc tctgagtagg tcaaggcctc caataatttc
     8101 tcacgtgcca catctgtcaa accttccagc tggtaaaggt ctgccttcag agccaaagac
     8161 gagacatacc agtcactgtc agcttggatt tcctcaagat aggtaaaggc ttcttctatt
     8221 tgaccatcct cgctagcaat tgcagctaga ttaagatgaa cctctggaaa atcctctaca
     8281 attttcaggt aaatttcctt ggcctgagga tagaaaccaa tcccttcaag ataagttgcc
     8341 aactcataca gaagatcact tgaatcattt tctaaagctt tggcgaaata atgctcagcc
     8401 ttagttaaat cttgctcctc caaagcctgt aacatctgtt gactattgtt cactcttgac
     8461 ttcctcaccc tctaattcat atagaccact gactacctta taccattcga aaatggctga
     8521 aatgacaacc ttagcagagg cataaaccgg aataccaagt aaaactcccc agataccaaa
     8581 catagatcct gaagttaaca aaacaaagag aacattaata ggatggatgt ttaattgact
     8641 tcccaaaatc aatggagaga caaaacggcc ttcaatagtt tgttctacga taaagacaat
     8701 cactactttc aaaagcatga ctggaccagc aatcaaaccc aatactagag caggaagcat
     8761 ggctagaaag ctaccaagat aagggaccag atttaaaata ccagcagtaa cccccagcgt
     8821 aaccgcatag cgtagaccaa taatcttgaa gaagatgata aacattactg ctacaataat
     8881 agccactgtc acttgccctc gaacatagtt ggacaactgt tgattcacat ctgataaaac
     8941 ttgtccaaca ggttccttca attttcttgg aatgaattgg gtcaaatagt tacgcaagcc
     9001 tttcccatca cgcaagagat aaaagagcat gaaaggaacg ataatcaagg caacaatcac
     9061 ttgagaagcc ccgctaataa aggcactcac ccagttgact gcctgagatg aaaccttact
     9121 tgccaaaact gtagcctggc tagaaaaatt ggtcaaaact tgctctaatt gaggtctgaa
     9181 atcatctggc aggtgctggg ctaccaatcc attaacaatc ctatctatat cttctaagta
     9241 aacaggaacg tttcttgcaa aggtcaaaac ctgacgttgc agatttggaa tggcgactgc
     9301 caagccccaa atgataaaga gagcgatgat aacaaagaca atagtgatag ctataacacg
     9361 attaacctta tgcttctcca tccaatcaac aataggattc aacaaataat ataacaaacc
     9421 agacaaaatg actggcaaca tcacaactgc taaaaagtct aaaacaggtg aaaatagaaa
     9481 actaatctta cttaaaataa aaagattcag tcccaataat aaggttacta aaaataccgt
     9541 aattgccttg ttatctaaaa accacttgaa aaaccaagat aggctaaaat gtttctcttt
     9601 ttgctccata aatacttcct ttctattgtt ctctcattat accatttttg aaacaaaata
     9661 actctttttt aaagtgctta catctaattg gatgtcttta atttgcttgt ttttgtgata
     9721 taatagtctt atctttacac agaggtatag taatgaaaga aactgtttat tttggaactt
     9781 atacacgtcg tacttctcaa gggatttaca aggcagactt tgatacagaa actggtcagc
     9841 tttcaaatct agaacttttt gcagctgagc caagtccaac ctaccttgcc tttgaccagc
     9901 accaacattt atacactgtt ggtagccaag acgataaggg gggaattgca gcctatcaaa
     9961 ctgacgggac tgtgttaaat catgttgttg aagaaggagc tccccactgt tatgttgctg
    10021 tcgatgaaaa gcgtgatttg gtttacgcag ccaactatca caagggacaa gtccttgttt
    10081 ataaacgcca ggaagatggt agtcttctac ttagtgatat ggatcaacac agtggccaag
    10141 gtccacatga aaatcaagct tccccccatg ttcactatac agatttaaca cctgaccact
    10201 atctagtgac ctgcgacttg ggaactgacc aagtcatcac ctatgacctc gatcaagaag
    10261 gaaaattatc taagctctat acctatcaca gcaagccagg agcaggctca cgccatatca
    10321 ttttccataa ccactataaa atcgcttatc tcatttgtga actcaatagt actatcgaag
    10381 ttttaatcta cgatggcgtt ggcgaatttg tacgtatgca ggttatttca actttaccag
    10441 aagcttacga aggctttaat ggaaccgctg ctattcatct ctctaaagac ggtaaatacc
    10501 tctacgcttc taaccgtggc catgattcta tcgcagtata taccatcctt gcggacggta
    10561 gcttagagtt attagaaatc gttccaacgc atggtcagac tccacgtgat tttgatttga
    10621 cccccgacca aaaatttctc attgttgtcc atcaagactc tgacaatgca actgtcttta
    10681 aacgtaattg tgacaatggt cgtctagcag aactctccaa cgacttccat gttcccgaag
    10741 cagtctgcat ccgttttgct ccttaaaaca ccataaaaaa tgaacttggt attttcaagt
    10801 tcattttttc ttatagtcta tttccgacat taatacggtt aatggcacgt tgcaaagcaa
    10861 tcttagcacg acgttcttgg tcaatcaaat gtttgtcttg tgcttcttca attgcacgtt
    10921 ctgcacgaag tttggcacgt tctgcacgac tgatatcgat atcacgagca cgttctgcag
    10981 agtcagcgac gattgtgatc atatcattgg caatttcaat aacgccaccg tttactgcaa
    11041 tccagttcac gtgatcttta tcatcgatac gttttacctt tacttcatca actgctaaaa
    11101 ccgcaatcat attttcatgt cgtggcaaga tccccatctc accatccaga gttcgaaccg
    11161 atacatagct ggcatggtga tcatagacga gaccatctgg tgtcacgatc tggacagtta
    11221 actgagccat aggtcacctc ttaaaattcc attttttcag cttttgcaat cacatcttcg
    11281 atagaaccta caccacggaa ggcatcttct ggcaagtggt cgtatttacc atcaaggatt
    11341 tccttaaagc cacgtacagt ttcagcaact ggaacataag aacctggctg accagtaaat
    11401 tgttccgcaa cgttgaagtt ttgtgacaag aagaactgga tacgacgggc gcgagcaacc
    11461 aaggtctttt cttcatcaga aagttcatcc ataccaagga tagcaatgat atcttgcaat
    11521 tcatggtaac gttgaaggac acgttttact tcagcagcaa ctgcatagtg ctcttctcca
    11581 acgatttcag gtgccaaggc acgtgagctt gaagcaagtg ggtcaacggc tgggtagata
    11641 cccaattgta ccaacttacg ttccaagttt gttgttgaat ccaagtgagc gaaggctgtt
    11701 gctggcgctg ggtcagtata gtcatccgct ggcacataga tagcctggat agaggttaca
    11761 gaacccttct tggttgatgt gatacgttct tgcaattgac ccatttccgt agcaagtgtt
    11821 ggttggtaac caacggctga tggcatacga cccaaaaggg cagatacttc tgaaccagcc
    11881 tgagtgaaac ggaagatatt atcgataaag agaagcacgt cttggccttc cacatcacgg
    11941 aagtattcag cgattgtcaa accagtaagg gcaacacgca tacgtgctcc tggtggctca
    12001 ttcatctgac caaagaccat ggctgttttc tcgataacgc ctgattcttt catttcccag
    12061 taaaggtcat tcccctcacg agtacgttcc ccaacaccag taaatactga aataccaccg
    12121 tgctcttggg caatgttgtg aatcaattct tggattaaga cggttttacc aactccggca
    12181 ccaccgaaaa gtccaacttt accaccttta aggtaagggg caagaaggtc aataaccttg
    12241 atccctgttt caaggatttc agaagaggta gacaactcat caaaagttgg agctttttta
    12301 tgaattggct gacgctctgc gtcttctgta aaaggagctt ccaaatcaat ggtatctccc
    12361 aaaacgttga agacacgtcc caaagtttct ttacctactg gtacagagat tggacgaccc
    12421 gtgtccaata cttccattcc acgagtcaac ccatctgttg attccatggc gatagtacga
    12481 accataccat ctcctaactc caaggctact tcaaggacga tttttgtttt tctttcgtca
    12541 tttttgtaga cgacaagtgc attgttaatc tcaggaagtt tttcccctgc tgcaaacaaa
    12601 acgtctacaa cgggaccgat aacctgagca attttacctg aactcatctc cttctcctat
    12661 tctatataag atactgtcgg ttcctagctc aactaggtcc taagttcatt ttcatacgag
    12721 ctggactaga gcctattcta aggcactagc acctgctacg atttctgtaa tttcttgtgt
    12781 aatcgccgcc tgtctggcac ggttatactg aattgtcaaa tcattgatga ctttcttagc
    12841 attatctgtc gctgtttgca tggctgtcat acccgcagca ttctcagctg tcttggcatc
    12901 gataatggca ccgtaaatca tactttctgc aaactgaggc aacaactgct ccagaatttc
    12961 ttctcggctg gtttccaatt caaaagtcaa gctgtactct tcatccgctt catttggatc
    13021 caagtcaaca atcggaagca tttgttccac acgcatttga ctggttagcg tattgacatg
    13081 gtggttgtag caaacataaa gctcatcaaa gagttcattt tggtacattt caacagtttt
    13141 tgaaataatc ttacgaactt gatcaaagct aggttggtct gccaagccac gtaattcata
    13201 aagtggttga ataccgcgag ccttaaagaa atcagctccc atcccaccga tacagatcat
    13261 ttcaaaacct ttaccgtctg ggtggtattc ttctttcaac tccataacag ctttcaaaat
    13321 agaggaatta taacctccaa ccaaaccgcg gtctgaagtg ataacgatat agcctgtctt
    13381 cttcacagaa cggctaatca acatcggatt agttgaagca ccagctccat taccatgaag
    13441 gatatctgtc aaaagtttac gcactttctg agcgtaaact tggaagttgc gagcagcttc
    13501 ttcagagcga cctaacttag cagccgatac catttgcatg gcattagtga tttgactcgt
    13561 attttttgtt gaggcgattt ttgttttaat atcatttaga gacactgcca tctgacacct
    13621 ctattcttat tggaagctag attgattgag aaactctgta atcgcagcat ccaagactgc
    13681 ttcttctggc aagtcttttg tatcacgaat ggtttccaaa atctctggat gttgagcatc
    13741 aaagaaggca tggaactctt cctcgaaacg aacaatatca tctactggaa cagtatccaa
    13801 gaaaccatgt gtcaaagcat aaagaatggt tacttgtttc tcaacaggta atggtttgtg
    13861 aacaggttgt ttcaagacct caacggtacg acgtccacgg ttcaacttag cctgtgttgc
    13921 tgcgtccaag tcagaaccaa acttagtaaa ggcttccaac tcacggtatg aagcaaggtc
    13981 gatacgaagt gtaccagcaa ccttcttcat ggctttgatt tgtgcagaac cacctacacg
    14041 agatacagat gaacccgcat cgatggctgg acgaatacct gcattgaaga ggccatcgcc
    14101 aaggaagatt tgtccatcag tgatagaaat cacgttggtt gcgatatagg ctgagatatc
    14161 tcctgcttgt gtctcgataa atggtagggc tgtaattgat ccaccaccaa gttcatcaga
    14221 aactttagct gagcgctcaa gcaaacggct gtggagatag aaaacatccc ctgggaaggc
    14281 ttcacgacct ggaggacgac gaagcaagag cgatagttca cgataagcta ccgattgttt
    14341 tgatagatca tcgtatacaa tcaaaacatg cttaccttga tacataaatt cttccgccat
    14401 agcaacccca gtataaggag ctaggaagag caatggagat ggttgtgaag cagaggctgt
    14461 cacaacgatt gtgtagtcca aggcaccgta ctgacgaagt gtttctactt gcgtacgaac
    14521 tgttgattct ttttgtccaa tcgcgacgta gatacagatc atatcttgat ctttttggtt
    14581 caagattgta tcaatcgcaa tggttgtttt ccctgtctga cggtcaccga taatcaactc
    14641 acgttgacca cgaccaatcg gtacaagggc gtcaatagct ttcaaaccag tttgcaatgg
    14701 ttctgaaaca gacttacgtt gcataacacc aggagctggt gcttctactg gacgagtttt
    14761 atcagtgtgg atttctccaa gaccgtcaac tggacgacca agcggatcca caacacgacc
    14821 aatcagactt tcacctacag ggacttccat gattttccct gtacggcgga ttgtatcgcc
    14881 ttcacggata tctgtaaagt cacctaggat gataatacca acgtctgttg actccaagtt
    14941 ttgagccata ccataagagc cgttttcaaa attcaacaac tctccactca tgacattttc
    15001 aaggccatga gcacgcgcga taccgtcccc gatataggtt acaacacctg tttcagtcac
    15061 atcaaaattg ggtttgaaat tttcaatttg ttgcttaatt aaagcgctga tttcttgtgc
    15121 gttaattgcc aaaagaacac cactttctat ttcaaatttt ctttaacaac tttaagttgt
    15181 tgtttaatac tcacatcaat tgtcttgtga ttggcaaaaa tgacaaaacc accaatgaga
    15241 ctttcatcga tttgttcttt tacactcctt actttcagag acattttttt ctcaatcaaa
    15301 gggagcaaac gagtcttctg ttcatcagtt agaggatgag cagacgtaat cgtcacttca
    15361 aatcgatttg tttctttttc aagtcggttc aagcaatcta caagcacatc ataaaaaaga
    15421 tttgctctgt gattgtaggc cagaacctgg ataaagtttt gtaataaagg cgacacagaa
    15481 tcttggaaaa aagcaattgt tttttcctta tccgactcgt ctactgccac ttgttttaaa
    15541 aaagaaggca gacctgtttt ttcaacaact tgcttgattt gagtcaagtc tgaaaagata
    15601 cggtcttctt ctcctttttc aagtaccaat tggacaaaag gcatgctgta tttttcaatt
    15661 acctttactg ttttcttgtc cattaagctt ctcctagctg atcgatatac tgatcaatga
    15721 gtgctttatg ggcatgactg tcaaggtttt gtgagatgat tttaccagct aagctgatgg
    15781 tcaaatctgc gacctcaccc ttaacactct gtaaagcttc tactttattt tgagcaattt
    15841 cttgattggc tttttctttt aagtgtcctg cttctagttt agcatctgct aagatattag
    15901 ccttactttg ctcagctgtt tcctttgcat tttcaatgat tgtcttagct tctttacggc
    15961 taccagccaa ttcatcttcg cgtttttgag ccaatacttc tgctttttga cgggcttctt
    16021 cagctctgtc aatatctgaa gcaatttttt cagctctttc ttcgaaaatg cctgtaatat
    16081 tagaccatgc aaatttttta atcaagacta gcaaaagaat aaaagagcca gtgattaaaa
    16141 taaaattacc aattaattca cctactgtta cgtgcatcag ttactccttt ctattcctct
    16201 tcaatattaa tcttattccc taaatacaca gatgtcaaaa gagtaaaaac ataagcttgg
    16261 atgcaggaaa taaagacaga aaatgcagtc caagccaaat tagctccaaa ggctactgga
    16321 taccaataaa tagcttggtg ggaaagaaga agtaacaaac tcgtcatgac ctctcctgca
    16381 aagatattcc caaaaatccg caaagccaaa gataagaagt ttgtaaattc ttccaagata
    16441 ttcatcggta tgacaaaaac aggactcata aaagatttta tacttttttt aaatccacga
    16501 cgacgaacgc tttctatatg tgtcaacaaa atgacaagaa aagataaggt taagtcatac
    16561 tgtaaattag cggttggcga actccaccag ttagtcccat cgatcgtttg aagctttgtc
    16621 attaagccaa ggttattggc aatcaccatg aaaaggaata aacaaaggaa aaagagtgag
    16681 taatctttca tgtagcgcga accaatgtta ggttctgtaa atccaataac aaagtcatag
    16741 acatactcaa gtacattttg ctttcctttg ggtttcaagg tcatattgcg gcttgcccaa
    16801 taaataaaga caaaaataac tcccacaatc aacaaagtca tggctaacat agtcagattg
    16861 aagataacag gaccaataga gatgattgga ttaatacttt cttccatcta aaatcctccc
    16921 ttttcttctc gttccatgta tttatttgat aataaatgag aagacaagag ttacaaagaa
    16981 agttccttca ataaaggcaa cacctaaaaa catcaaacta cgaaactcag aaagcatatc
    17041 tggttggcgt gctactgatt taaacagtcc attcatcaat aaaccttcac cgacagatac
    17101 gcccatacag gcaatacata agcctaaaaa tgttaaattc ataacaaatt ctcctttaaa
    17161 ataaaattta cttacttagt gtagtcctaa aatttaaatt tgtcaacttt taaccgctat
    17221 tggaagcgtt taataaaaat tatttccatt t
//
LOCUS       DAVBCN010000043        16071 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVBCN010000043 DAVBCN010000000
VERSION     DAVBCN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16071
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.043"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            63..527
                     /locus_tag="VQ497_001311"
     CDS             63..527
                     /locus_tag="VQ497_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HET2517615.1"
                     /translation="MGGHPGFNCLLLDDEVYEDYYLEFENEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            685..1110
                     /gene="lacA"
                     /locus_tag="VQ497_001312"
     CDS             685..1110
                     /gene="lacA"
                     /locus_tag="VQ497_001312"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HET2517616.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            1141..1656
                     /gene="lacB"
                     /locus_tag="VQ497_001313"
     CDS             1141..1656
                     /gene="lacB"
                     /locus_tag="VQ497_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HET2517617.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            1667..2596
                     /locus_tag="VQ497_001314"
     CDS             1667..2596
                     /locus_tag="VQ497_001314"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HET2517618.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDDQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKLVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLEELKEVLQESLFTGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAGLLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            2598..3578
                     /gene="lacD"
                     /locus_tag="VQ497_001315"
     CDS             2598..3578
                     /gene="lacD"
                     /locus_tag="VQ497_001315"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HET2517619.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKILVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            3812..4648
                     /locus_tag="VQ497_001316"
     CDS             3812..4648
                     /locus_tag="VQ497_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET2517620.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENH
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALIQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            4684..5001
                     /locus_tag="VQ497_001317"
     CDS             4684..5001
                     /locus_tag="VQ497_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2517621.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            5003..6697
                     /locus_tag="VQ497_001318"
     CDS             5003..6697
                     /locus_tag="VQ497_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2517622.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIF
                     ILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTN
                     QINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
                     VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFS
                     ATDGYIGLAIVFGAYAFFWFVGIHGPSVVEPLIVAISYANIEANVQLVQAGMHADKIL
                     NPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSF
                     VLAITLIIIDTLIYYPFVKVYDHQILEEERSGKSNDELKEKVAANFNTAKADAILEKA
                     GVDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLP
                     EFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQAQFD"
     gene            6906..8312
                     /gene="lacG"
                     /locus_tag="VQ497_001319"
     CDS             6906..8312
                     /gene="lacG"
                     /locus_tag="VQ497_001319"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HET2517623.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPLDPKNPSDVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            8355..8537
                     /locus_tag="VQ497_001320"
     CDS             8355..8537
                     /locus_tag="VQ497_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HET2517624.1"
                     /translation="MFKDFIQSIYEKVYIINFEKCSQIPCLTSEELKSLFLNFINPEE
                     WDTISFDSDFMPFQQS"
     gene            complement(8815..9576)
                     /locus_tag="VQ497_001321"
     CDS             complement(8815..9576)
                     /locus_tag="VQ497_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2517625.1"
                     /translation="MLKQEKLAKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNVSNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(9758..10720)
                     /gene="nrdF"
                     /locus_tag="VQ497_001322"
     CDS             complement(9758..10720)
                     /gene="nrdF"
                     /locus_tag="VQ497_001322"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HET2517626.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(10908..13067)
                     /gene="nrdE"
                     /locus_tag="VQ497_001323"
     CDS             complement(10908..13067)
                     /gene="nrdE"
                     /locus_tag="VQ497_001323"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HET2517627.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(13148..13366)
                     /gene="nrdH"
                     /locus_tag="VQ497_001324"
     CDS             complement(13148..13366)
                     /gene="nrdH"
                     /locus_tag="VQ497_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HET2517628.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            13717..13980
                     /locus_tag="VQ497_001325"
     CDS             13717..13980
                     /locus_tag="VQ497_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HET2517629.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            13986..15719
                     /gene="ptsP"
                     /locus_tag="VQ497_001326"
     CDS             13986..15719
                     /gene="ptsP"
                     /locus_tag="VQ497_001326"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET2517630.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            15974..>16071
                     /locus_tag="VQ497_001327"
     CDS             15974..>16071
                     /locus_tag="VQ497_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2517631.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTV"
BASE COUNT         5024 a         2819 c         3331 g         4897 t
ORIGIN      
        1 aactagaatt ctagttttgc aggaagtcta ataaagaaac tagcaaggtc aggccttact
       61 ttattggtgg acatccagga tttaattgtc ttctacttga tgatgaagtc tatgaagatt
      121 attatctaga gtttgagaac gaagagattt gctctgttcc acgtcctttc ccagaaacag
      181 gtatgttgga tttccaagat agaagtccat ggctagaggg acaaaaagaa atagatctta
      241 gttatgatct tttcagcaca gatgcagtga ctttggatga attgcaatct cgaacaattg
      301 cccttcgttc tcttaaacat gataagggat tgaaagtgca ttttgcagaa tttccaaacc
      361 tcatcatctg gtcaactttg aacaagggac ctttcattac ctttgaacca tggtctggct
      421 tgtcaacatt ccttgaagaa ggagatcatt tagaagataa gaagaatgtt tgtctcttag
      481 aagccaatca ggttgaagaa ttagggtttg agatagaagt tttataaaaa aagtaaaaaa
      541 taaataacaa aaacaaacac aaactgttga cttcttcaaa caatagtagt atattatgtt
      601 tgtgaggact aattgactgt tttttaaaga cagccaatac actgctggaa tccagcatag
      661 aaaaaataaa aaggagtata caatatgtct attgttatcg gtgcagatgc tgcaggtttg
      721 agattgaaag aagttgtgaa agacttctta gaaaaagaaa acttccacct tgtggatgtt
      781 acagctgaag gtcaagactt tgttgatgtg actctcgctg ttgctgcaga agtaaacaaa
      841 gaagaacaaa accttggtat cgtgattgat gcttatggag ctggtccatt tatggttgca
      901 actaagatca aaggaatggt tgctgcagaa gtatctgacg aacgttcagc ttatatgact
      961 cgtggccaca acaactcacg tatgatcact atgggggcac aacttgttgg tgatgaattg
     1021 gctaaaaaca tcgctaaagg atttgttaat ggtaaatacg acggtggacg tcatcaaatc
     1081 cgggttgaca tgttgaacaa aatgggctaa ttagattaca gtaagaaagg taagttaaaa
     1141 atgagaattg caattggatg cgaccacatc gtaacagatg aaaaaatggc ggtttcagaa
     1201 tttttgaaat caaaaggata tgaagtcatt gactttggta catatgacca tacacggact
     1261 cactacccaa tctttggtaa aaaagtaggg gaagctgtaa ctagcggcca agctgatctt
     1321 ggggtatgta tctgtggtac tggtgttggt atcaacaacg ctgtaaataa agttccaggt
     1381 gttcgttctg ccttggttcg tgatatgaca acagcccttt atgctaaaga acaattgaac
     1441 gctaacgtta tcggctttgg tggtaagatt actggtgaat tgcttatgtg tgatatcatc
     1501 gaagctttca ttcatgctga atacaaacca tctgaagaaa acaagaaatt gattgcgaaa
     1561 attgaacacc ttgaaagtca caatgctcaa caaacagacg caaacttctt tacagaattc
     1621 cttgaaaaat gggatcgcgg agaataccac gactaagagg tgacctatga ttttaacagt
     1681 cacaatgaac ccatccattg atatttccta tcccttggat gagttgaaga ttgatactgt
     1741 caatcgtgtg gtggatgtaa ccaaaacggc tggtggtaag ggacttaatg ttacccgagt
     1801 actttcagaa tttggtgatt ctgttcttgc tactggttta gtgggtggca aacttggtga
     1861 gtttttggtt gaacatatcg atgatcaagt aaagaaagat ttcttttcta ttcagggaga
     1921 aactcgtaac tgtatcgcta ttctccacgg tgacaaccaa acagaagttc ttgaaaaagg
     1981 tcctgaagta ttggaacagg aaggtcaaga ctttttggaa catttcaaaa aactcttgga
     2041 gtcagttgaa gtagtagcca tctcaggtag tctgccagct ggccttccag ttgattacta
     2101 tgcgagcttg gtagagcttg ctaatcaagc tggcaagcta gtagtcttgg actgctctgg
     2161 tgcagcactt caggcggttc ttgaatcacc ccataaaccg acagtaatca aacccaataa
     2221 tgaggaattg tctcagcttc taggaagaga agtttctgag gatttggagg aattaaaaga
     2281 agtccttcaa gaatctttgt ttacagggat tgaatggatt atcgtttcac ttggtgccaa
     2341 cggtactttt gccaaacatg gtgacacttt ctacaaggta gatattccta gaattcaggt
     2401 ggtaaatcct gttggatctg gagactctac tgtggcagga atttcttcag gacttcttca
     2461 caaagaatcg gatgcaggat tactcatcaa ggcaaatgtc cttggtatgc tcaatgctca
     2521 agaaaaaatg actggtcatg tcaacatggc caactatcaa gctctatatg atcaattaat
     2581 agtaaaagag gtataaaatg gctttaacag aacaaaaacg tgtacgctta gaaaaacttt
     2641 ctgatgaaaa tggtatcatc tcagctcttg catttgacca acgtggtgct ttgaaacgcc
     2701 tcatggctca acaccaaaca gaagaaccaa ctgtggccca aatggaagaa cttaaaatct
     2761 tggtagcaga tgaattgact aaatacgctt catctatgct tcttgaccct gagtatggac
     2821 ttccagcaac taaggctctt gatgaaaaag ctggtcttct ccttgcttat gaaaaaacag
     2881 gttatgacac aacaagcaca aaacgcttgc cagactgctt ggatgtttgg tctgcaaaac
     2941 gtattaaaga agaaggtgca gatgcagtta aattcttgct ttactatgat gtagatagct
     3001 cagacgaact caatcaagaa aaacaagcct acatcgaacg catcggttct gagtgtgtgg
     3061 ctgaagatat cccattcttc cttgaaatcc ttgcttacga tgaaaaaatt gcggacgcag
     3121 gttctgtaga atacgctaaa gtaaaaccac acaaagttat cggtgctatg aaagtctttt
     3181 cagacccacg ttttaacatt gatgtcttga aagttgaagt tcctgttaac attaaatatg
     3241 ttgaaggctt cgctgagggt gaagtggttt acacacgtga agaagcagca gccttcttca
     3301 aagcgcaaga tgaagcaacg aacttgccat acatctactt gagtgctggt gtatcagcta
     3361 aactcttcca agatactctt gtatttgctc atgaatcagg tgcaaacttt aacggagttc
     3421 tttgtggtcg tgctacatgg gcagggtcag ttgaagctta catcaaagat ggtgaagcag
     3481 cagctcgcga atggcttcgc acaactggat ttgaaaacat tgatgagctc aataaagttc
     3541 ttcaaacaac agcgacttca tggaaagaac gtgtgtaaga aagtcctcct agtttaggaa
     3601 catgaatcta aaaaaattta aaaaaagttg tatgtaaagg tttacaaaat aacttacttg
     3661 tgctatactt aaatcacaag ttaatacaag gtgagtgtta ctaagtaata ttaggcatga
     3721 tcacaggtga attagaaatc agctgatttt ctagttcatt tgtggtcatt ttttgtactc
     3781 atataccttt aagatataaa aggaggttga catgtatcga attctaaatc caatgaatca
     3841 caatgtctcg cttgtcagaa atgataaggg agaagaggtg attgtaattg gtaaggggat
     3901 tgcattcgga aagaagaagg gggatttgat tgctgaaaat catgttgaga aaatctttcg
     3961 gatgaagacc gaagagtcca gagaaaactt tatggctctt ctcaaagatg ttccgcttga
     4021 ttttatcaca gtgacctatg aaatcattga taagctatca aagaaatatc attatccgat
     4081 tcaagagtat ctctatgtaa ccttgacaga tcatatttac tgttcttatc aagctctaat
     4141 tcaaggaagg tacaaggata gtaatctgcc agatatttct gctaagtatc ctgtcgcttt
     4201 tcaaatcgca aatgaagctt ttgaaattta ccgtcagaag ctagcagatc attttcctga
     4261 ggacgaaatt attcggattg cttatcattt cattaatgct gaaggtgaaa atgaagtgga
     4321 acttgtggag tcgattgata agaggaaaga aattctcagg aatgttgaag aagttttaac
     4381 ggactatgca attcaacgaa ctaaaaagaa taaccatttc tatgatcgct ttatgatcca
     4441 tttgaattat ttcttggatt atttagacag atctagagat gataaccaat cacttctgga
     4501 tatggaagat catattaaac aatcctatcc aaaagccttc gagattggtt ccaagatcta
     4561 tgatgtgatt acgcaacata cgggtcttga tttgtataaa agtgaacgag tttatctagt
     4621 tctacatatc caacgtttat tgtcataaaa atttatttaa aactatataa ggagaattct
     4681 atcatgaata gagaagaagt aacattgtta ggttttgaaa tcgtagccta tgctggcgat
     4741 gctcgttcaa aactattgga agccttgaag gctgctgaag ctggtgattt tgaaaaagcg
     4801 gacgctctgg tagaggaagc tggtagctgt attgcagagg ctcaccacgc gcaaacaagt
     4861 ctattgacta aggaagcttc tggtgaggac ttagcgtata gtgtgaccat gatgcatggt
     4921 caagatcact tgatgacaac tatcttgtta aaagacctaa tgcaccattt aattgaactt
     4981 tacaagagag gagttaaata aaatggataa gttaataatt tttattgaga aaggaaagcc
     5041 tttctttgag aaattatcga gaaatatata cttaagagcg ataaaagatg gttttatttc
     5101 cagtatgcca gcagtattat tctcaagtat ttttatacta atagctgctg tgcccaatat
     5161 ttttgggttt aaatggtctg atgaacagtt agcttttatt ttgaaacctt acaactactc
     5221 aatggggatt ttagctctct tagtagctgg gacaactgca aaatcgttaa cagattcagt
     5281 taatacacgt agtatggaga aaaccaacca aatcaactac atgtcaactt ttttagcagc
     5341 tgttgttgga ttgttaatac tggcagctga tccaattgaa ggtggatttg ctaatggtct
     5401 tttaggtact agaggattac ttactgcatt tttggcagct tttattacag taaatatcta
     5461 taaagtatgt attaaaaata atgtaaccat tagacttcct gaagaggttc caccaaatat
     5521 tgcacaagtt tttaaagatg ttattccgtt tgccttatct gttctttcaa tatatggatt
     5581 ggatttaatt gttagaaata tttttggtac aaatgtagca gaatctgttg gtaaaatatt
     5641 agcccctcta ttttcagcta ctgatggtta tatcggttta gctattgtat ttggtgctta
     5701 tgctttcttt tggtttgttg gaattcatgg gccatctgtt gtagaacctt taattgtagc
     5761 tatcagttat gcaaatatag aagctaatgt tcagcttgtg caagctggta tgcacgcaga
     5821 taaaatttta aatccagtta ctcaaacttt tgttgtaaca atgggtggta ctggtgcaac
     5881 actagtagtt ccatttatgt ttatgtggtt atgtaaatca aaaagaaata gaattgtcgg
     5941 acgtgcgtct gtagttccta ctttctttgg cgtaaatgaa ccaatcctat ttggtgctcc
     6001 aattgtttta aatcctatct tctttattcc gtttgttact gctcctatta tcaatgtttg
     6061 gattatgaaa ttttttgtag atgttctaca aatgaatagt ttcagtatta ttttaccttg
     6121 gacaacacct gctccaattg gtattgttat gggaacggcg ctggctccat tgtcattcgt
     6181 cttggctata actttaatca ttatcgacac tttaatctat tatccatttg ttaaagttta
     6241 tgaccatcaa attttagaag aagaacgttc aggtaagtct aatgatgaat tgaaagaaaa
     6301 agttgctgca aacttcaaca ctgcaaaagc ggatgctatt cttgaaaaag cgggtgtcga
     6361 tgcagcacaa aataccatca ctgaagaaac aaatgtcctc gttctctgtg caggtggagg
     6421 aacaagtggt ctccttgcaa atgctttgaa taaggcagca gcagaataca atgtccctgt
     6481 gaaagcagca gcaggcggct atggtgctca ccgtgaaatg ttaccagagt ttgatcttgt
     6541 tatccttgcc cctcaagttg cttcaaactt tgaagatatg aaagcagaaa cagataagct
     6601 cggtattaaa ctagcgaaaa cagaaggcgc tcaatacatc aaattaactc gtgatggaaa
     6661 aggtgctctt gcattcgtac aagcgcaatt cgattaaggc tagagactct gaaatagtct
     6721 cctatcgtta cggaaatcgc tatggcgaat ttcctattat taattcgtcg gtaaaaagat
     6781 atcgttttta cctcctcatg tcacaattcg gtgacttggt acaagaagtg agatggagaa
     6841 ggatggctca ctgactcctc tcctctcact tttactttat ttaaatcaag aaataggtga
     6901 aaaaaatgac aaaaacactt ccaaaagact ttatttttgg tggcgcaaca gctgcctatc
     6961 aagcagaagg tgctacacat actgatggta aaggaccagt agcgtgggat aaatatcttg
     7021 aagataacta ctggtacact gcagaaccag ccagtgattt ttacaatcga tatccagttg
     7081 acctcaagct agcagaagag tatggtgtca atggtattcg aatttctatt gcttggtcac
     7141 gtattttccc gactggttac ggccaagtaa atgctaaagg tgttgagttt tatcataatt
     7201 tatttgcaga gtgtcacaaa cgtcatgttg agccctttgt aactcttcat cactttgaca
     7261 cgccagaagc tctccactca aatggagact tcttaaaccg tgaaaatatc gaacattttg
     7321 tagactacgc tgccttctgt tttgaagaat ttccagaagt aaactattgg acaaccttta
     7381 atgaaattgg gccaattggt gatggtcaat acttggttgg gaaattccct ccaggtattc
     7441 agtacgacct tgccaaagtc ttccaatcgc accacaatat gatggtgtct catgcgcgtg
     7501 cagttaagct atataaagat aaaggctata aaggcgaaat tggtgttgtt cacgctctgc
     7561 caactaagta tcctttagat cctaaaaatc cgtcagatgt tcgtgcagct gagttggaag
     7621 atattattca caataaattt atcttggatg caacttatct aggtcgctat tcagctgaaa
     7681 cgatggaagg tgtcaaccat atcttatcag tcaatggtgg tagcttggat cttcgtgaag
     7741 aagattttac agcattagaa gcagcaaaag acttgaatga tttccttgga atcaactact
     7801 atatgagtga ctggatggaa gcctttgatg gagaaactga aattatccat aatggtaagg
     7861 gtgagaaagg aagttctaaa taccaaatca aaggtgttgg tcgtcgtgta gctcctgatt
     7921 atgtaccacg tacggattgg gattggatta tctaccctca aggtttatat gaccaaatta
     7981 tgcgtgtgaa gaaagattat cctaactaca agaagattta catcactgaa aatggtctcg
     8041 gctataaaga tgagttcgtc gataacactg tttatgatga tggacgtatt gattatgtga
     8101 aacaacactt ggaggtcttg tctgatgcga ttgcagatgg agcgaatgta aaaggttact
     8161 tcatttggtc attgatggac gtcttttcat ggtcaaatgg ttatgaaaaa cgttacggtc
     8221 tcttctatgt agactttgaa actcaagaac gttatccgaa gaaatcagct cactggtaca
     8281 agaaagtagc ggaaactcag attatagact agtagaatta gtcattagat atagaatttt
     8341 agtgagtcaa aaagatgttc aaagatttta tccaatctat ttatgaaaaa gtttatatta
     8401 taaatttcga aaaatgctct caaataccgt gtttgacgag tgaagaattg aaaagtctat
     8461 ttttaaattt tatcaatcct gaagaatggg atactatctc ctttgattca gattttatgc
     8521 catttcaaca atcgtaacca atttctcaaa aaagttaaat cttatattta gtactctgca
     8581 aaactcttat ctaatcacgt tgcttatact caatgaaaat caaagagcaa ctttaaacta
     8641 ggaagcgagt cgcagatttc tcaatgcata gctttgagga attgggcaaa aagtctttga
     8701 tatagaaaaa gcatagtatc aggtgtttca acacctgata ctatgctttt tattgtggga
     8761 agatttactt tttttcttct gaaattgagt tgttacccag gctctttcag tttattaagg
     8821 cttgatgact ttaatgtgtt tagatagctt aaaaaggatt gaatcactta gtttagaatc
     8881 tgaaacaata gtatcaagat ttgatacatt ataaaaagta taaaaatcaa acttattgaa
     8941 cttactatga tctgcgagta aatatttttt attagaatta tttaaagcga tgcgttgagc
     9001 ctctccctct tcctcgctaa aagtagctag agctccgttt tgaataccat tacagctaac
     9061 gaaagcttta gaaaattgga gattagagag attttgtagg gtcaatgtgc caacaaaagc
     9121 acctgtaata tcgcgataat ttccacctat caaaatcaaa tctgttaatt ttcgttcgct
     9181 taaaatcaga aaaacaggta gactgttggt tacgacgcgg atattgtcaa taggcaactc
     9241 acgcgcaaaa aactctaatg ttgttcctgg tccaatgaaa atagtttctc tttcttctac
     9301 tagactgcct gcaaaatggg ctatttcttg tttttctgcc gtttggaggg cttgtttttc
     9361 aatatttgat cgctcattag tcaaaaggga gttggttcga agtttttcag ctccaccatg
     9421 cacacgaatc agcaaatctt tatcagctaa ttcctgtaaa tagcgccttg cagtcatatc
     9481 tgaaacggct atttcgtcca taatctgttt aactgttatg gttcctttac tatttactat
     9541 ctctaaaatt ttggctaatt tttcttgttt gagcatatta tcacctcgtt tcctactact
     9601 atcttaccat aaacaaactc atcattcaaa tacaaaaaca acaaaatgaa acaaaaacaa
     9661 aaatatcgaa gtttgttttc aaaactttcg atatttttgt tgggttataa ctttgatgtt
     9721 tctagtttac tttttgatga ttgagagtga tggagaatta gtctaaaccg tagttatagt
     9781 catcgtcttg catggcttca acttcgccaa gaaggtaacc atttccgact tgagagaaga
     9841 agtcatggtt ggaagttcct gttgaaatac cgttcataac gattgggttg acatcttcag
     9901 ctgaatctgg gaaaagtgga tcttgtccca tgttcatgag agctttattg gcattgtagc
     9961 gaaggaaggt tttaacctct tcagtccaac caacaccgtc ataaagactc tctgtgtagc
    10021 cttcttcatt ttcataaaga gtatagagta ggtcgtacat ccattctttg agtttttctt
    10081 gctcttcttc aggcaattca ttgaaaccaa gttggaattt gtaaccaatg taggttccgt
    10141 gaacagactc gtcacgaata atcaatttaa tgatttctgc aacgttggct agtttgttgt
    10201 taccgagata gtagagggga gtgaagaaac cagagtagaa gaggaaggtt tcgaggaaga
    10261 cgctggcaac tttcttttca agtgggctgc cgtttaggta gatttcgttg acaatctcag
    10321 ccttcttttg taggtaagga ttggtattgg tccattcgaa aatttcttca atctcagcct
    10381 tagtattcaa ggtagaaaag attgatgagt aagatttagc gtggacagat tccataaatt
    10441 ggatgttatt gaaaacagct tcctcatgtg gtgtacggat gtctgcgcga agggcttgaa
    10501 ccccagtttc agattgcata gtgtcaagaa gggttaaacc accaaaaact tttccgacca
    10561 agtctttctc tttgttagat agctttctcc agtcatccaa gtcgtttgat aagggaatac
    10621 gtgtatcgag ccaaaattgc tccgtcagtt tttcccaagt tgatttgtcg atgacatctt
    10681 cgatggcatt ccagttaatg gctttgtagt aagtttccat ttaaaatctc tttctgtgtt
    10741 tagtattgcg aactcacaat tatttctact ttaccataat tctataggag tatcgcacaa
    10801 aaagtcggaa gcccgacttt taaaatgtta cataaattat gttatgacat agtagatttg
    10861 attttatcag tgctgcttag ggaaaaataa tgtttctatg ctagaaacta aatcacacag
    10921 ctttcacatt ggttggcgcc gacttctcca ccgtcatctg taaaggtacg gacgtagtag
    10981 atagacttga ttcccttgtt aaaggcatag ttacgaagga tggacaagtc acgtgtcgtt
    11041 tgtttatttt ccctcttcca ttcgtaaagg ccttttggaa tgtcactacg catgaagagg
    11101 gtgagtgaaa gtccttgatc cacgtgttca gtcgcagcag cgtaaacatc gatgacttta
    11161 cgcatatcca tatcgtaggc agaagtgtag taaggaatgg tttctgtaga caagccagca
    11221 gcagggtagt agattttacc aattttcttc tcttggcgtt cttcgatacg ttgcgtaatc
    11281 gggtggatag aagcagaaac gtcgttgata tagctgatag aaccatttgg cgctacagca
    11341 aggcgatttt ggtggtaaag accatcttct tgaaccttgt cgcgaagttc agcccaatca
    11401 gcaacaccag ggataaagac atttttgaag agttctttaa cacggtctga tgttggaaca
    11461 aattcaccag ttacatactt gtcaaagtaa cttccgttag catagtctga tttttcaaag
    11521 ttgtggaagg taataccacg ttcacgcgca atattgtttg actctaccaa ggtccagtag
    11581 ttcataagca taaagtagat gcttgtaaat tcaacagact caggtgaacc atattcaatg
    11641 agttgttggg caaggtagct gtgcagtccc atggcaccga gaccaaaggt gtgggcttgg
    11701 ctatttccat ggtcaatcgt tggtacagct acgatatgtg aactatctgt aacgaaagta
    11761 agggcacgaa ccatagcacg gatagaacga ccaaaatcag gtgaagtcat catgttaacc
    11821 acgttggttg aacccaggtt acatgaaaca tctgttccca tttgaaggaa ttcttgagca
    11881 tcgttgatca agcttggttc ttgaacttga agaatctcag aacacaagtt actcatgata
    11941 atctttccat caacaggatt tgcacggtta gccgtatcga tgttgactac ataaggatag
    12001 ccagactctt gttgcaattt agagatttca gtttccaaat cccgcgcctt gatttttgtc
    12061 ttgcgaatat ttggatttgc gaccaattca tcgtattttt cagtaatgtc gatgtaattg
    12121 aatggcacac cgtattcttt ttctacagag taagggctga agaggtacat ttcttcattt
    12181 ttacgagcca attcgtagaa tttatcaggt actacaacac caagtgatag agtcttgaca
    12241 cgtacttttt catcagcgtt ttctttctta gttgaaagga aagcgatgat atctgggtga
    12301 aagacgttga ggtagacaac accggcacct tgacgttgcc ccaattggtt ggagtaagag
    12361 aagctgtctt caaaaagctt cataacagga acgacacctg aagcagctcc ttcatagcct
    12421 ttgataggtg caccagcttc acgaaggttg ctgagggtaa ttcccacacc accaccaata
    12481 cgtgaaagtt gaagagctga gttgatagaa cgtccgatag agttcatatc atccgtcact
    12541 tggattagga aacaagatac caactctcca cggcgagcac gtccagcatt caagaaggaa
    12601 ggagtagcag gttggtagcg ttggtggatg atttcattgg caatatcgat tgcaacagct
    12661 tcattcccat cagcgaaata aagggcgttg aagaagacac ggtcttccat attttcaaga
    12721 tagtattcac cgtcattagt ctttaaggca tattgattgt aaaatttata agctgccatg
    12781 aatgacttga attggaagtt ttggtctttg ataaattgag ctaattcttc caagaactct
    12841 ggacggtatt tcttgataaa ggctgtttcg atgtagttgt gttcaatgag gtaattgatt
    12901 ttgtctttga ttgaatcaaa aaccatagtg tttggaacta cattttcttt aaagaaagca
    12961 tccaaggctt ccttgtcttt atgaagcatg atttgtccat taacaggacg gttaatttcg
    13021 ttattaagac ggaagtaagt cacgtcttca agatgtttta atcccataaa atttccctta
    13081 tctaattaca aaagaaaggc ttctaagtta gccctaaaag cagtttcttc tggatgatgt
    13141 actaagatta tgctaattgt ttcagttttc ctggttggaa acctgaaaag acttcagttg
    13201 gtgtttggat aacaggagct gcgctaaaac cgagctcttt aacttgatcg acgtactcag
    13261 gttgctcatc aagattgatt tcacgataag agacattatt actgtccaag aaacgcttgg
    13321 tcattttaca ttggacacaa ttgtttttag aataaacggt taccattgtg taactcctct
    13381 tcaaaattta atactatctt agtatatcag aaaataaaat tttgtcaatg atttgaacta
    13441 aatatagtgg tgttcttttg ttgtttttat atataaacac aatatatagt gtttgtattg
    13501 tgaaatagca atgattatcc tataaattgt ttttaaagaa actatatcct gtgttttgtt
    13561 atctaggata gaagattttc agcaagttat ctgaaaggaa attgaaggaa aatccagaaa
    13621 atgcttgaaa aaaatcctag aagatggtat aatactaaat tgtaagggtt atcacatata
    13681 actcaaaaaa agaaagaaca aaaggagagt caaactatgg cttctaaaga tttccacgta
    13741 gtggcagaaa caggtattca cgcacgtcca gcaacattgt tggtacaaac tgctagcaaa
    13801 tttgcttcag atatcactct tgagtacaaa ggtaaatcag ttaaccttaa atcaattatg
    13861 ggtgttatga gtcttggtgt tggccaaggt gctgacgtaa ctatctcagc tgaaggtgca
    13921 gatgcagatg acgctatcgc tgcaatctca gaaacaatgg aaaaagaagg attggcataa
    13981 gggaaatgac agaaatgctt aaaggaatcg cagcatctga cggtgttgca gttgcaaaag
    14041 catatctact cgttcagccg gatttgtcat ttgagactat tacagtcgaa gatacaaacg
    14101 cagaagaagc tcgccttgat gccgctctac aggcatcaca agacgagctt tctgttattc
    14161 gcgagaaagc agtaggtacg ctcggtgaag aagcagctca agtttttgat gctcacttaa
    14221 tggttcttgc tgacccagaa atgatcagcc aaatcaagga aactatccgt gcgaagaaag
    14281 tgaatgcaga agcaggtctg aaagaagtta cagatatgtt tatcactatc tttgaaggca
    14341 tggaagacaa cccatacatg caagaacgcg cagcggatat ccgcgacgtg acaaaacgtg
    14401 tattggcaaa ccttcttggt aaaaaattgc caaacccagc ttctattaat gaagaagtga
    14461 ttgtgattgc gcatgacttg acaccatctg atacagctca attggacaaa aactttgtaa
    14521 aagcttttgt aaccaacatt ggtggacgta caagccactc agctatcatg gcacgtacac
    14581 ttgaaattgc tgctgtatta ggtacaaata acatcactga aatcgttaaa gacggtgaca
    14641 tccttgctgt taacgggatc actggagaag tgattatcaa cccaacagat gaacaagcgg
    14701 cagaatttaa agcagctggt gaagcctatg cgaaacaaaa agctgaatgg gcacttttga
    14761 aagatgctca aacagtgact gctgacggta aacacttcga gttggctgcc aatattggta
    14821 ctccaaaaga cgttgaaggt gttaacaaca acggtgcaga agctgttgga ctttaccgta
    14881 cagagttctt gtacatggat tctcaagact tcccaactga agatgagcag tatgaagcat
    14941 acaaggctgt tcttgaagga atgaacggta aacctgttgt cgttcgtaca atggatatcg
    15001 gtggagataa ggaacttcct tacttcgata tgcctcacga aatgaaccca ttccttggat
    15061 tccgtgctct tcgtatctct atctctgaga ctggagatgc tatgttccgc acacaaatcc
    15121 gtgctcttct tcgtgcgtct gttcacggtc aattgcgtat catgttccca atggttgcgc
    15181 tcttgaaaga attccgtgca gcgaaagcag tctttgatga agaaaaagca aaccttcttg
    15241 ctgaaggtgt tgcagttgcg gataacatcc aagttggtat catgatcgag attcctgcag
    15301 cggctatgct tgcagaccaa tttgctaaag aagttgactt cttctcaatt ggtacaaacg
    15361 acttgatcca atatacaatg gcagcagacc gtatgaacga acaagtttca tacctttacc
    15421 aaccatacaa cccatcaatc ctacgcttga ttaacaatgt gatcaaagca gctcacgctg
    15481 aaggtaaatg ggctggtatg tgtggtgaga tggctggtga ccaacaagct gttccacttc
    15541 ttgtcggaat gggcttggat gagttctcta tgtcagcaac atctgtactt cgtacacgca
    15601 gcttgatgaa gaaactcgac acagctaaga tggaagagta cgcaaaccgt gcccttacag
    15661 aatgctcaac aatggaagaa gttcttgaac ttcaaaaaga atacgttaat tttgattaat
    15721 cgaaaagtcc ctgcaactca gttacaggga ctttttttga tattttaaaa agaattttca
    15781 agaaaatctt tcttataaaa agtccaacct tgaaaaagta gtggtcagaa caaaaaatac
    15841 ttaaatggtt cataaaattc ttgacaagtt ggagatttag gagtaaacta ttaaccagtt
    15901 aagtaataga gaggagtttc tgcaatttag aaatgaattg caactagaaa tatcaaatag
    15961 aaagagagtt tcgatgaaaa ttaataagaa ataccttgtt ggttctgcgg cagctttgat
    16021 tttaagtgtt tgttcttacg agttgggact gtatcaagct agaacggtta a
//
LOCUS       DAVBCN010000044        16069 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVBCN010000044 DAVBCN010000000
VERSION     DAVBCN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16069
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.044"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(365..1138)
                     /locus_tag="VQ497_001328"
     CDS             complement(365..1138)
                     /locus_tag="VQ497_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2517632.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTLPDQKLLDAVELFDGEWVSDIQ"
     gene            1314..2717
                     /locus_tag="VQ497_001329"
     CDS             1314..2717
                     /locus_tag="VQ497_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HET2517633.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEATAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            2832..3470
                     /locus_tag="VQ497_001330"
     CDS             2832..3470
                     /locus_tag="VQ497_001330"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HET2517634.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMELMAEKFKTYGQRK"
     gene            3482..3922
                     /locus_tag="VQ497_001331"
     CDS             3482..3922
                     /locus_tag="VQ497_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HET2517635.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            3915..4346
                     /locus_tag="VQ497_001332"
     CDS             3915..4346
                     /locus_tag="VQ497_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2517636.1"
                     /translation="MFNRGILVASHGDFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVDELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISNYDSKINLEEIVHNAQNSLFNVKQQLNVEEEEDLCL"
     gene            4337..4807
                     /locus_tag="VQ497_001333"
     CDS             4337..4807
                     /locus_tag="VQ497_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2517637.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            4832..5605
                     /locus_tag="VQ497_001334"
     CDS             4832..5605
                     /locus_tag="VQ497_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2517638.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            5602..6402
                     /locus_tag="VQ497_001335"
     CDS             5602..6402
                     /locus_tag="VQ497_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2517639.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            6461..8755
                     /locus_tag="VQ497_001336"
     CDS             6461..8755
                     /locus_tag="VQ497_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HET2517640.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            8765..11881
                     /locus_tag="VQ497_001337"
     CDS             8765..11881
                     /locus_tag="VQ497_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HET2517641.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVPFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDSNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNNHEVFKQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            11938..13704
                     /locus_tag="VQ497_001338"
     CDS             11938..13704
                     /locus_tag="VQ497_001338"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HET2517642.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVERVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            13850..15001
                     /locus_tag="VQ497_001339"
     CDS             13850..15001
                     /locus_tag="VQ497_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET2517643.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGRPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            15146..16030
                     /locus_tag="VQ497_001340"
     CDS             15146..16030
                     /locus_tag="VQ497_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET2517644.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
BASE COUNT         5288 a         2368 c         3433 g         4980 t
ORIGIN      
        1 tatatttaga tcatcaaggt cacttaattc ttgaaatata gggtctaggc gacaaagtct
       61 ttaaaatcag aaaaacgcat aatatcaggt gtgcaaaaac ttgatactat gcgttttatt
      121 gtgggaaggt ttactccatt ttctcctgaa attgagtttt tgtccagcct ctgtttttag
      181 aattgctaag aaaataatgt catgtggtga atatttgtaa atcagtcagc agacagaacg
      241 aatactcttc gaaaatctct tcacatcatg tcagcttcgt cttgccgtgt ccatggggga
      301 cttgacttga tcagttttca aatctgtact ttgagcaagc tgagactagc ttcctatttg
      361 attttcattg aatatcagaa acccattctc catcaaataa ttcgactgcg tctaataatt
      421 tttggtctgg caaggtgtca gaaataaagg ttgtgtattt ggagagggga ttaattttaa
      481 aaaatccagt cttgtaaaat ttagaactat caatcagtaa gatggtttca tgggctttgt
      541 caataatatt cttttttgaa atagcttggc tgagagaagc ttcataaaca tattggtcat
      601 caatacctct tgctgaacaa aatgctaaat cgatattaaa atgatctaat aaagaatttt
      661 ccttatcata gttgaccacg gaacaggatt gatgtttgac ctcgccagat gtgataaaga
      721 ttttggagct atctttaaca gtttcagata gggtttgtgc agtatgtaaa ccatttgtaa
      781 aaataatcaa attatcaagt tcagaaagat agggacaaag ttcgtagaca gtagtactag
      841 aatctagata gatacacata ccagaccgaa taaagtcttt agcgagacta gcgattagtc
      901 ttttttgcct agtactttct ccttcccgta tttgatgaga aagttcaatt gtgttcatag
      961 aggacagggt cacgtatccg tgctttcttt tgataagacc ttgattttct aagaaaatta
     1021 aatcacgacg taaggtactt gtgctggaga aagtgatttc tgccagctct tttacggcaa
     1081 ttcttttttt ctttttaata atttcaatca attcaagtac acgttcatct tttatcataa
     1141 gctcctccta atttatcatt tcaactatat tatagcacaa attggaggaa tttgaattat
     1201 ttttatgaat attgggttaa catttgaaca ttattcaagt aagcgttcac atattgaaaa
     1261 aataaaacgt ggggattata ataaagttaa tcaaggacga agagagaaga aaaatggaag
     1321 cggttttagc aatagattta ggtgcgactt ctggaagagc aatcgttggt tacctttctg
     1381 aaaataaact agtaatggaa gaaataaatc gcttttctaa tctacctatt agagtaaaag
     1441 ggcatttatc ttgggatatt gactttctac tagctaaaat tcttgaaagt atccgcttgg
     1501 ctaatactag ttacaagatt ttatctatcg gtattgacac atggggagtt gattttggac
     1561 tgattgataa tgaaggtaag ctgttatcac aacctgttca ttatcgtgat gaaagaacaa
     1621 agggagtgtt aaaggaaata tctgaaatga ctgaattaga aaaactgtat tcagagacag
     1681 gaaatcagat tatggagata aataccttgt ttcaactctt taaggcacgt caagaatctc
     1741 ctgactcttt ctataagacc aataagattc ttttaatgcc agatttgttt aattatctct
     1801 tgacaggtaa gtttgctaca gaaaaaagca ttgcttcaac aactcaatta tttgatccta
     1861 ggagtcaaaa ttggaatcag aatatcttaa aactatttga attggattca tctttacttc
     1921 ctgaaattgt ttcagaggga aatgttcttg gaaggataaa aaaggagtat ggtttaggcg
     1981 atattcctgt tgtgaatgtt tgtagtcatg atacagcaag tgcgattgtc tcagtaccta
     2041 agacagaagg tagtttattt atttcatcag gtacttggtc tttggttgga gtggaactta
     2101 cttcaccgat tcttactacc gaatccttca gttatggatt tacaaatgaa gtcggtaaag
     2161 atggagtgat tacatttctg aagaattgta cagggttgtg gatcatagag gaactaagac
     2221 gttcatttga acgaagaggg aaagcctatt cttttgatga tattaggaca atggtggaga
     2281 aagaaaaaga aaatcttcct ctgattgata ctgaatcaac tgaatttgca acagaatctg
     2341 atatgcacaa gactttgaca gaatatctag cttatcatca tgaaactaga gagtggacag
     2401 atggacaact atttaagatt gtttatgaaa gcctagctga aacgtatagg aaagcgatag
     2461 agttattaga agaactaact cataaggttt ataagaggat atatgtgatt ggaggaggtg
     2521 ctagagccag ttattttaac caaatgattg ctgatagaac tggtaaagag gttcttacag
     2581 gtttgactga ggctacagct gtggggaata ttgttgtgca gctcatagct atgggacaat
     2641 taaaagggat ggaagaggct caccatgtta ttgaggagtt tctacaatta gagagttatt
     2701 actcccaaaa gaattaaaaa gattgagagt ttgtaaattt gcctccctcc cccttcttag
     2761 cttttgtgca ggaagggggg ataattggtg aattgaaaaa tatttagtgt tttgatatga
     2821 ggaggacaag gatgtcagat gtaaaacaag aattaattaa atatggtaag aagctagtag
     2881 aaacagattt gacgaaagga acaggtggga atctcagcgt tttcgatcgt gaaaaacaat
     2941 tgatggcaat taccccgtcg ggtattgatt tctttgaaat caaagaatcc gatattgtag
     3001 tgatggatat taatggaaat gttgtagagg gagaacgctt gccatctagc gaatggtata
     3061 tgcatttgat tcaatatcaa actcgtgatg atatcgatgc aattatccat gctcatacaa
     3121 cttatgcaac agtattagct tgtctcagag aaccacttcc agcgagtcat tatatgattg
     3181 cagtggcagg gaaagatgtt cgggtagctg agtatgcaac atatggcacg aaagaattgg
     3241 ctgtgaatgc agctaaagca atggaaggtc gtagagcagt tttactagcg aatcatggaa
     3301 ttttagcagg tgcacaaaat ttattgaatg catttaatat tgttgaagaa gttgaatatt
     3361 gtgcaaaaat ttattgttta gctaagaatt ttggagagcc agtagttctt cctgatgagg
     3421 agatggaatt gatggcagaa aaatttaaaa catacggtca gagaaaatag ggaggatatt
     3481 aatgttaaaa catataccga aaaatatttc tccagattta ttgaagactt taatggaaat
     3541 gggacatgga gatgaaatag tattagctga cgcgaattat ccttctgcct catgtgcaaa
     3601 taagctaatt cgttgtgatg gcgtaaatat tccagaatta ttagattcca ttctgtattt
     3661 aatgccatta gatagttacg tcgatagttc aattcagttt atgaacgttg tttcgggtga
     3721 tgatattcct aagatatggg gtacctatag acagatgatt gaaggtcatg gtacagatct
     3781 taaaacgatt acttatctta gaagagaaga cttttatgaa cgtagtaaga aagcttatgc
     3841 tattgttgct acaggagaaa cttcacttta tgctaatatt atccttaaga aaggagtagt
     3901 tgttgaaaga gaaaatgttt aatagaggaa ttttagttgc cagtcatggt gattttgcta
     3961 gcggagctct catgaccgca gaaatgtttg ttggtgagac aacaaatgat agagttagga
     4021 cattaggttt gatgcctgga gagaatattg tagagtttga gcattatttt aaaaatcaag
     4081 tggatgaact gttagactca aatcaagagg ttatcgtttt gactgacttg attggaggaa
     4141 gtcctaataa tgtggctttg tcacggtttt taaatttgga ttcagttgat attgtaacag
     4201 ggtttaatat tcctctccta gtggaattaa tatcaaatta tgattcaaaa atcaatttag
     4261 aagaaattgt tcacaatgct caaaatagtt tgtttaatgt taaacaacaa cttaacgtag
     4321 aggaggaaga agatttatgt ctatagagtt tgttcgtatt gatgaccgtc tggtacatgg
     4381 tcaagttgtc actacgtggc taaaaaagta tgatattgag caagttatca ttgttaatga
     4441 tcgcatctca gaagataaaa cacgacaatc tattttaaag atttctgcac cagtaggttt
     4501 aaaaattgtt ttctttagtg taaaacggtt tgtggaagtt ttaaactctg tgccaataaa
     4561 aaagagaaca atgctgatat atacaaatcc aaaagatgtg tatgattcta ttgaaggaaa
     4621 tttaaaattg gagtacctca atgtaggaca gatgagtaaa acggaggaaa atgaaaaggt
     4681 aacgggaggt gtagctctag gtgaagaaga caaatattat tttaagaaaa tagttgataa
     4741 gggaacgaga gttgaaattc aaatggttcc taatgataaa gttacaatgt tggaaaaatt
     4801 tttataaaaa taatttaagg aggtacagta tatgctattc acacaagcat tactggtgac
     4861 attagttggg attattgcca ctattgacta taatggaccg ttatttatga ttcaccgtcc
     4921 gttagttaca agtgcaatgg ttggcttagt cttaggagat ttcacccaag gtgttcttat
     4981 tggttcagct cttgaattaa cttggctcgg tgtaacaggt attggaggtt acactccacc
     5041 agatactatt tcaggtgcga ttattggtac tgcatttggt attttatctg gtcaaggaga
     5101 aactgctggt atcgctatag cagttccaat tgcagttgct acccaacagt tggatgttct
     5161 tgcaaaaact ttagatgttt attttgtgaa aaaagctgat aatgatgcta aaaacggaga
     5221 ttattcaaag atcggttttt atcattattc aagtttggtt ttaatcacgt tatttaaaat
     5281 tgtaccaatt ttcctagcta ttatgcttgg aggggaatat gtggcagact tgtttgctaa
     5341 ggttccacca atcgttatgc agggacttaa ctctgcaggt gctttactac cttcaattgg
     5401 ttttggtatg cttttaaata tgatgctcaa gaaaaatatg tgggtattct tgttgattgg
     5461 attcatttgt tctgtgtatg gaggaatgtc aaccattggg atctcactag ttggtattgc
     5521 ggtagcatac ttctacgata tgattggaag taaaccacaa gaaacaactt caagtagtga
     5581 tgttgaggag gatcttgatc tatgatgaat aataaagtaa ctaaagttga acttaaaaaa
     5641 gttttcaaac gaagttttat gtatggttct tcatggaact atgagagaat gcagaaccta
     5701 ggttttctat atacaattct tccagtattg aaaaaactat acccagacaa agattcagct
     5761 tctcctgcaa tgaaacgtca ccttgagttt ttcaatactc atcaaacagc ggcaccattt
     5821 attcttggag ttacttccgc tatggaagaa caagaaggaa atgaaggtgc agcttcaatt
     5881 actggtatta aagttggctt gatggggcca ctggctggtc taggagatag tttgttctgg
     5941 ctgacactag ttcctatctg ttttagtatt ggtgcgtctt attctaaaga cggcggtgct
     6001 ttaggtatct ttatcgcctt aatattgttt aatattatta atattcctgt taaatatttc
     6061 ggtttgaaat atgggtatac taagggttct agtcttatcc aagaaaataa tacaaaagga
     6121 acattgaatc gcgttacgag tatggcgaca gcattagggc tagtactagt gggtggtttg
     6181 attccatcaa tggttggtat taattttgga ttagaattta agcaggggga acttgttatt
     6241 tctgttcaag aaatgattac aaaattaatt ccaggattta tccctatggc tttgacttta
     6301 ttaatgtgta aattaattag aaaaggaaag aatccggttg tactaatctt tagtgttatg
     6361 gctattggag ttatcctagt tgttttagga attttgaagt agtagaaagt gtggaggtgg
     6421 tatttgggat atcacctcca ttttggaaga gaggtaaaga gtgaaattat ggtataagaa
     6481 agctgcctca aattggaatg aagccttgcc gattgggaac ggtcatttag gtggtatgat
     6541 ttatggttca gctacaaaag aatgtattca actaaacgat gagactattt ggtatagagg
     6601 aaagtcagat agaaataatc cagactcact attgcatctt aaaaaaattc gggaatatct
     6661 tttagatgga gaaattcaga aagccgaaga attgataaag ttaacaatgt ttgctacccc
     6721 aagagatcaa agccactatg aattacttgg ggaactttac attgagcata tagatattca
     6781 gtcttgtgct ctttcattgt atgaaagaga gctagattta gatacagcta tttctaatgt
     6841 tgtgtttgag cctaatagtt gtaatttaca aataaaaaga gaatatttta cgagttttaa
     6901 taagaatatt ttatgttgcc gtatagtgtc atcagttcaa aacacattaa atttaaacat
     6961 taatttgggt agaaataaac ggtttaatga cgaagtatct aaactggatt caagtacaat
     7021 tttaatgtcg gcctctgctg gaggtagaaa aggtgttcag tttaaagtag tatgtcattc
     7081 taaggttacg gatggtgaag taagtgtatt gggagagaca atagttattc ggaatgctac
     7141 agaggtattt ctttatctca aatcaatgac ggattattgg ggaaatatag atatttcttc
     7201 tcttcaggga gaatttagta gtattgatta ctttacagaa aaagatgaac atgtaaaaaa
     7261 atatcaggag caatttaata gagttgattt taaactagac tatagtaaag attgtcttag
     7321 cattccaacg aatctacttc ttgaaaacac taaaaagtat agtaactact tgactaactt
     7381 gttatttcat tatggaagat atctgttaat atcgtctagt caaccgaatg gtttacctgc
     7441 caatcttcaa ggaatatggt gtgatgaatt aaatccaatt tggggttcta aatatacgat
     7501 taatattaat actcaaatga attattggat ggtaggtcca tgtgatttac cagaagtaga
     7561 atatccatta tttgatatgc tcgaaagaat gagagaaccg ggaagactaa ccgctaagaa
     7621 aatgtatgga gctagaggtt ttacagcaca tcataatacg gatggttttg gcgatacggc
     7681 tccccaatct catgccatgg gggctgcaat ttgggtatta actattccat ggttatgtac
     7741 tcatatttgg gaacactatt tatatttcca agatgagcgt attcttacgg aacattttga
     7801 aatgataaaa gaagcatttc ttttctttga agattattta tttgaggtgg atggctactt
     7861 gatgacaggt ccaagtgtct caccggaaaa taaatatcgc ttaaaaaatg gtattgaagg
     7921 aaatgcttgt ctatcatcta caattgataa tcaaattcta agatattttt gtgattcatg
     7981 cattggcatt gcaaaacaat taggagacaa ttcggatttt attagtcgtg tgaaggagtt
     8041 aaaaaagaaa ctacctaaaa caaaaatagg tagtaatggg caaatccaag aatggttaga
     8101 agattatgaa gaagtagagc ctgggcatag acacatttca cctctatttg ggctttatcc
     8161 ttataatgag attgatattc ataaaactcc ggaattagca gaagcagcta aaatcactat
     8221 caataggaga ttatcaaacg ctaacttttt atcttcacag gagagggagc aagcgattaa
     8281 taattggtta gtaagtggtt tgcatgctag tacacaaaca ggttggagtg ctgcatggct
     8341 gattcatttt tttgcgagac tatatcaagg tgaacctgct tataaccaga ttaatggttt
     8401 gttaaataat gcgactcttg gcaatttatt tcttgaccat ccaccatttc aaattgatgg
     8461 taatttaggt ttggtgagtg gaatttgtga attattagta cagagccatc ataattggtt
     8521 atcactaatt ccagctttac cttctgcttg gtcagaagga gaagtgaaag gtttcagagt
     8581 aagaggagga tataaggtat cgtttgcttg gaaaaatggg gatataacat tcctaaaatt
     8641 ggaaggagga aacaaagatc aaaaagtaag agtaagaata tatggcaaaa atactgatgt
     8701 acaaaatatt gaattggtat ttaattcaga aaaaattatt gagttaaatt tttaggtata
     8761 agtcatgaat aaagaaaaaa taaaaagaaa attaatcaca atactgtttg tatgtattgg
     8821 gatgttatgt tttggattgt tagcaggagt taaggctgat aatcgtgttc aaatgagaac
     8881 gacgattaat aatgaatcgc cattgttgct ttctccgttg tatggcaatg ataatggtaa
     8941 cggattatgg tgggggaaca cattgaaggg agcatgggaa gctattcctg aagatgtaaa
     9001 accatatgca gcgattgaac ttcatcctgc aaaagtttgt aaaccaacaa gttgtattcc
     9061 acgagatacg aaagaattga gagaatggta tgtcaagatg ttggaggaag ctcaaagtct
     9121 aaacattcca gttttcttgg ttattatgtc ggctggggag cgtaatacag ttcctccaga
     9181 gtggttagat gaacaattcc aaaagtatag tgtgttaaaa ggtgttttaa atattgagaa
     9241 ttattggatt tacaataacc agttagctcc gcatagtgct aaatatttgg aagtttgtgc
     9301 caaatatgga gcgcatttta tctggcatga tcatgaaaaa tggttctggg aaactattat
     9361 gaatgatccg acattctttg aagcgagtca aaaatatcat aaaaatttgg tgttggcaac
     9421 taaaaatacg ccaataagag atgatgcggg tacagattct atcgttagtg gattttggtt
     9481 gagtggctta tgtgataact ggggctcatc aacagataca tggaaatggt gggaaaaaca
     9541 ttatacaaac acatttgaaa ctggaagagc tagggatatg agatcctatg catcggaacc
     9601 agaatcaatg attgctatgg aaatgatgaa tgtatatact gggggaggca cagtttataa
     9661 tttcgaatgt gccgcgtata catttatgac aaatgatgta ccaactccag catttactaa
     9721 aggtattatt cctttcttta gacatgctat acaaaatcca gctccaagta aggaagaagt
     9781 tgtaaataga acaaaagctg tattttggaa tggagaaggt aggattagtt cattaaacgg
     9841 attttatcaa ggactttatt cgaatgatga aacaatgcct ttatataata atgggagata
     9901 tcatattctt cctgtaatac atgagaaaat tgataaggaa aagatttcat ctatattccc
     9961 taatgcaaaa attttgacta aaaatagtga ggaattgtct agtaaagtca actatttaaa
    10021 ctcgctttat ccaaaacttt atgaaggaga tgggtatgct cagcgtgtag gtaattcctg
    10081 gtatatttat aatagtaatg ctaatatcaa taaaaatcag caagtaatgt tgcctatgta
    10141 tactaataat acaaagtcgt tatcgttaga tttgacgcca catacttacg ctgttgttaa
    10201 agaaaatcca aataatttac atattttatt gaataattac aggacagata agacagctat
    10261 gtgggcatta tcaggaaatt ttgatgcatc aaaaagttgg aagaaagaag aattagagtt
    10321 agcgaactgg ataagcaaaa attattccat caatcctgta gataatgact ttaggacaac
    10381 aacacttaca ttaaaagggc atactggtca taaacctcag ataaatataa gtggcgataa
    10441 aaatcattat acttatacag aaaattggga tgagaatacc catgtttata ccattacggt
    10501 taatcataat ggaatggtag agatgtctat aaatactgaa gggacaggcc cagtcccttt
    10561 cccaacacca gataaattta atgatggtaa tttgaatata gcatatgcaa aaccaacaac
    10621 acaaagttct gtagattaca atggagactc taatagagct gtggatggta acagaaatgg
    10681 taattttaac tctggttcgg taacacacac tagggcagat aatccctctt ggtgggaagt
    10741 cgatttgaaa aaaatggata aagttgggct tgttaaaatt tataatcgca cagatgctga
    10801 gactcaacgt ctatctaatt ttgatgtgat tctatatgac aataatagaa acgaagttgc
    10861 taagaaacat gttaataatt tgtcgggtga atctgttagt ctagatttca aagaaaaagg
    10921 agcaaggtat attaaagtta aattactaac gagtggagtg cctttgagtt tagcagaagt
    10981 agaggttttt agagaatcag atggtaagca atctgaagag gatatagata aaataacaga
    11041 agataaagta gtctctacaa ataaggtagc tactcaaagt tcaaccaatt atgagggtgt
    11101 agctgcttta gcagttgatg gtaataaaga tggagattac ggacatcatt cggtgactca
    11161 tactaaggca gattctaacg cttggtggca ggtcgatctg ggagaagagt ttacggtttc
    11221 taaagttgat atttataata gaacagatgc cgaacctcag cgtttatcta attttgatgt
    11281 tatttttcta tcttcatcag gagaagaagt ttttagaaga cattttgata aagtagttga
    11341 tggtttgtta tctttaaaag taccttctgt aggggctaag ctagtcaaaa tagaattaaa
    11401 atcagcagct attccgttaa gtttagcgga agttgaagtc tatggttcaa agagaactcc
    11461 gaagaaactt tctaatattg cattaaccaa agaaactcga cagagttcaa cggattacaa
    11521 tggtttttct cgtctagcag ttgatggaaa taaaaacgga gattatggtc atcattcagt
    11581 gactcatacc aaaggagatt ctccttcatg gtgggagata gatttagcac aaaccgaaga
    11641 attagaaaag ttaattattt ataatagaac agatgctgaa attcagagat tatcaaattt
    11701 tgatattatt atatatgatt caaataatca tgaagttttt aaacaacata ttgacagttt
    11761 agaaagcaat aatctatcca tagacttaaa agggctgaag ggaaaaaagg ttagaatttc
    11821 tttgagaaac gcaggaattc ctttaagttt ggcagaggta gaggtttata cttataagta
    11881 attttaaaaa ttatcatcgg tagtatgaaa gaaacagaaa aataagagga aaatagtatg
    11941 attcaacatc cacgtattgg tattcgtccg actattgatg gtcgtcgtca aggtgtacgc
    12001 gaatcacttg aagtacaaac aatgaacatg gctaaaagtg tggcagattt gatttcaagc
    12061 acattgaaat atccagatgg ggaacctgtg gaatgtgtga tttctccatc taccattggt
    12121 cgtgttccag aggctgcagc ttcccatgag ttgtttaaaa aatcaaatgt ttgcgcaaca
    12181 attacagtta caccatgctg gtgttatggt agtgaaacta tggatatgtc tccagatatt
    12241 cctcatgcta tttggggatt taatgggaca gaacgcccag gagctgtcta tcttgcagct
    12301 gtactagctt cacatgctca aaaagggatt ccagcctttg ggatttatgg tagagatgtt
    12361 caggaagcta atgatacaga tattccagaa gatgtcaaag aaaaactttt acgttatgcg
    12421 cgtgcagctc ttgcaactgg cttgatgaga gacactgctt acctatcaat gggtagtgtt
    12481 tcgatgggga ttggtggttc tattgtaaat ccggatttct tccaagaata cttaggaatg
    12541 cgaaatgaat cggtagatat gacggagttc acacgccgta tggaccgtgg tatttacgac
    12601 cctgaagagt ttgaacgtgc tatggtttgg gtaaaagaac atatcaaaga aggagttgac
    12661 cgaaatcgtg aggatttgat tctttctaaa gaagagaaag aaaaacaatg ggaatttgtt
    12721 attaagatgt tcatgattgg acgtgactta atggttggta acccaagact tgctgaactt
    12781 ggttttgagg aagaagcagt tggtcaccat gctttagtag ctggtttcca aggtcaacgt
    12841 cagtggacag accattttcc aaatggagat tttatggaaa ctttcctcaa tactcagttt
    12901 gactggaatg gtattcgaaa accatttgta tttgcgacag agaatgattc actaaatggt
    12961 gtgtctatgc tctttaatta tctattaaca aatactccac aaatctttgc tgatgtgcgt
    13021 acttattgga gtccagaggc tgttgaacgt gtaacaggac atactttaga gggtcgtgct
    13081 gcagctggct tcttacatct aatcaactct ggatcttgta cattggatgg tacaggtcaa
    13141 gctactcgag atggcaaacc tgttatgaaa ccattctggg agttggaaga aagtgaagtg
    13201 caggctatgc ttgaaaatac agacttccca ccagcaaacc gcgaatactt ccgtggagga
    13261 ggattctcaa ctcgtttctt gacgaagggg gatatgccag taacaatggt acgtctcaat
    13321 cttctaaaag gggttggtcc agtgctacaa attgcagaag gttacacact tgaacttcct
    13381 gaagatgttc accatacttt agataatcgt acagatccag gatggccaac tacttggttt
    13441 gctccacgtt tgacaggaaa aggtgctttc aagtctgtct atgacgtcat gaataattgg
    13501 ggagctaatc acggagccat aacatatggc cacattggag cagacttgat taccttggct
    13561 tctatgttga gaattcctgt caatatgcat aatgtacctg aggaagatat ctttagacct
    13621 aaaaattggt ccttatttgg aacagaagat ctagaatcag cagactatcg tgcatgtcag
    13681 ttgttgggac cactacataa ataaaacttg tttatatagg aggtgaactt acgtccctcc
    13741 tatcctttta aaaagatttg ttaaacaatt cacaaataat tgaaaacgaa tacaaaaagt
    13801 aatataatga tgttaaatag atagcgcaaa ggcgcaggag gaaaattata tggctacatt
    13861 ttatgttccg gcagtcaacc ttattggtaa aggtgttgta aatgaagtgg gtccttatat
    13921 caaggaactt ggctataaaa aggcactttt ggttacagat aagtacatcg aaggcagtga
    13981 cattttacct aaggttttaa aaccactgga tacagaagga atcgaatatg tcatctttag
    14041 cgatgtagag ccaaacccta cttgtaagaa tgtcacagat ggggtagctg ctttgcaaga
    14101 acatggatgt gattttatca tcagtcttgg tgggggttct ccacaggatg cagctagttg
    14161 tatttctatc atggctacaa atggtggaag accacaggat tatgaagggc ttcataagtc
    14221 tgctaaaaaa ggcttgccag ttgtggctat caatacaacg gcaggtacat cagcagaaat
    14281 taccattaac tatgtgatta ctgatgaaga acgcaaggtt aagatggtaa tggttgacaa
    14341 gaatagcctt gccttgattt ctgtcaatga ccctgaactc atgctttcca aacctaaagg
    14401 cctgactgct gctactggta tggatgctct gactcatgct gttgaagctt tggtaacacc
    14461 tggtgcttat gatgtaacca agaaactgtc tattggtgct attgagctta tcaaggaata
    14521 tcttcctcgt gctgtagaaa atggacatga tattgaagcg cgtgaaggta tggtcaatgc
    14581 catcttcctt ggtggtatga gctttaataa tgctggtctt ggctatgttc actcaatggc
    14641 tcaccaactc ggtgcagtat ataatttgcc acatggcgtg tgctgtgcca tgttgctacc
    14701 agttatagaa cgtgaaaatg ctaaacgtgt accagaagct ttccgcaatg ttgccaaagc
    14761 cttgggactt catgtagaag gtaaatcaga tcaagaatgt gccgattatg cgattgctga
    14821 gattgagaag ctttctgaga cagtaggtat tcctaagaaa ctgactgaac ttggtattga
    14881 agaaaaagat ttcgactttg aatacctttc taagaatgcc ttgattgatg cctgcgcacc
    14941 aggaaatcca tttatgccaa ccttagaaga aacgattgcc ttttataaag agttatttta
    15001 gcaagagatt ggacgacaat aggacaattc acctctaatt attgacattt caggaaaaat
    15061 cgcagtaaaa acggcacagc cttctttaaa tgtgctataa tactagaaaa atacttgtgg
    15121 aggttccatt atggcaatat ttttcatgat ttttctgatt gtttgtgtgc tcctattggt
    15181 gatagtcaca ctgagtacag tttatgtggt tcgtcagcag tcggtggcga ttattgaacg
    15241 ctttgggaaa taccaaaagg ttgccaatag cggtattcat attcgcttgc cttttgggat
    15301 tgactcgatt gcagcacgga ttcagttgcg cttgttgcaa agtgatattg tggttgagac
    15361 taagaccaag gacaatgtgt tcgttatgat gaatgtagcg actcagtacc gtgtcaacga
    15421 gcagagcgtg acagatgctt actataaact catacgtcca gaatctcaga ttaaatctta
    15481 tatcgaagat gctcttcgtt cttctgttcc aaaattaacc ttggatgaat tgtttgagaa
    15541 aaaagatgag attgcccttg aagttcaaca ccaagtagca gaagaaatga ccacttacgg
    15601 ctacattatc gtgaaaacct tgattaccaa ggtcgaacca gatgcagaag ttaagcaatc
    15661 tatgaatgaa atcaatgcgg cgcaacgtaa gcgggtcgca gcacaagaat tggcggaagc
    15721 tgacaagatt aaaattgtca ctgcagctga agccgaagca gaaaaagacc gccttcatgg
    15781 tgtggggatt gcccaacaac gtaaggcgat tgtggatgga ttggcagagt ctatcaccga
    15841 actcaaggaa gccaatgttg gaatgacaga agaacaaatc atgtctatcc tcttgaccaa
    15901 ccagtatttg gataccttga atacctttgc ctctaaagga aatcaaacca tctttttacc
    15961 aaatactcca aatggtgtgg atgatatccg aacacaaatc ttgtcagccc ttcgcgctga
    16021 gaagaaataa tagactaata ctcttcgaaa atctcttcaa accacgtca
//
LOCUS       DAVBCN010000045        16041 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVBCN010000045 DAVBCN010000000
VERSION     DAVBCN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16041
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.045"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(115..1356)
                     /locus_tag="VQ497_001341"
     CDS             complement(115..1356)
                     /locus_tag="VQ497_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HET2517645.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITAYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFIDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            1459..2568
                     /locus_tag="VQ497_001342"
     CDS             1459..2568
                     /locus_tag="VQ497_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET2517646.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            3102..3449
                     /gene="ssrA"
                     /locus_tag="VQ497_001343"
     tmRNA           3102..3449
                     /gene="ssrA"
                     /locus_tag="VQ497_001343"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(3632..3719)
                     /locus_tag="VQ497_001344"
     tRNA            complement(3632..3719)
                     /locus_tag="VQ497_001344"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3683..3685),aa:Ser,seq:gga)
     gene            3881..4621
                     /locus_tag="VQ497_001345"
     CDS             3881..4621
                     /locus_tag="VQ497_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2517647.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNITVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            4618..5529
                     /gene="pfkB"
                     /locus_tag="VQ497_001346"
     CDS             4618..5529
                     /gene="pfkB"
                     /locus_tag="VQ497_001346"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HET2517648.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDVVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            5526..7478
                     /locus_tag="VQ497_001347"
     CDS             5526..7478
                     /locus_tag="VQ497_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2517649.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            7642..9867
                     /locus_tag="VQ497_001348"
     CDS             7642..9867
                     /locus_tag="VQ497_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET2517650.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(9912..10340)
                     /locus_tag="VQ497_001349"
     CDS             complement(9912..10340)
                     /locus_tag="VQ497_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517651.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQSNAPTSENKSNQ"
     gene            10478..11620
                     /locus_tag="VQ497_001350"
     CDS             10478..11620
                     /locus_tag="VQ497_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2517652.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWLIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRHDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     NYPDIFVFSDEENFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            11629..12843
                     /gene="thiI"
                     /locus_tag="VQ497_001351"
     CDS             11629..12843
                     /gene="thiI"
                     /locus_tag="VQ497_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HET2517653.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            12978..13802
                     /locus_tag="VQ497_001352"
     CDS             12978..13802
                     /locus_tag="VQ497_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2517654.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPGQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYFECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            13824..14563
                     /locus_tag="VQ497_001353"
                     /pseudo
     CDS             13824..14563
                     /locus_tag="VQ497_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            14576..15742
                     /locus_tag="VQ497_001354"
     CDS             14576..15742
                     /locus_tag="VQ497_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET2517655.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIGRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMEQMIADFGQRQE"
BASE COUNT         4667 a         3006 c         3522 g         4846 t
ORIGIN      
        1 acctgcggct agcttcctag tttattcttt gattttgagt attagattta ctcaaaatca
       61 aggattttga agatgaactt gtatcaaaaa atcgcggttt aaaatcgcga ttttttataa
      121 tttctcgtta acaaagcgga caaactgatt ccaccaaact tttaagaaga aggctttttc
      181 aattttcttg tctgctacca tttcgaaact agggcgctct gtggtgatgt aaccttgacc
      241 aatcaagtcc ttgtcttcat aagtcaaatg gccaaccact gttccagctt caagtggtgc
      301 tgggattgct ttggaatcag gtgtgaattg aacagattgg gaagattgat tcccaacacg
      361 ttcgattaga tagatatcct ctggagccac tgcagttact gtatcttctt ttccatcttg
      421 tacaggggct ttgctatctt gataggcatc gccttgttga acgattttgc gaagtgtaaa
      481 tgtagaagaa atataatcca ttagggaaga tgtagctgta aatcgagcgt aaggattatt
      541 gtcttgatga tctgcattta aaacaactgt gatgactctc atgccttttt cgacagtagt
      601 accaacaaaa gactctccag ccttatctgt tgttcctgtt tttagcccat caaaaccacc
      661 acggtaagca ggcatacctt ctaacatgta gttggttgaa gtgattgtca tcccagcaaa
      721 agtagaagaa ggttttttgg tgatttctaa gacttgtggg tattttttga tgaggttgcg
      781 agcaacgata gcgacatcat aagcactaag cttattttcc tcatcttttt tagaacctgg
      841 gtaaatgtta tcccctagag tttcattgtt aagacctgtc gtattgacaa cagtggcatc
      901 ctgaattccc cattccaaga gttttgcccg catcatatcg atgaaatctt tttctgagcc
      961 agcaattttc tcagctaggg caatagcggc gctgttggca ctagatacca gagttgcttc
     1021 aagcaactct tcgacagtat aattacgggc ctccatagga atattactgg cttcagaatt
     1081 tgtcgtcaat tgataaggat aatcagaaat atctacagga gtggagaggg taatacttcc
     1141 gttttccaaa gcttcataga ccagataagc agtaatcaat tttgttatgg aagcaatttc
     1201 gacaggttgc gttgcatcct tctcatagag aattttacca gtatttgcct caacagcaat
     1261 cgcatgttta gcggcaatgg taaaatcttg agcaacagca gtagaagcac cccctaaaag
     1321 agagacagtt aacaaagtta aaaatatttt tttcatagta gtcttattct atcataaaga
     1381 aaaaaaatat tcttgcttta ataattcatc tgttaagctt tttgaaaata tggtaaaata
     1441 aagtgaggga ggtaactcat gtttcgtaga aataaattat ttttttggac cacagaaatt
     1501 ttactcttaa ccatcatctt ttacctatgg agacagatgg ggtctttgat taaccctttt
     1561 gttagcgtgc ttaatacaat tatgattcca tttttattag ggggctttct ttattatttg
     1621 acaaacccta ttgttacttt cttaaataaa gtctgtaaac tcaatcgttt gcttggtatt
     1681 ttaattacct tgtgtacttt ggtctgggga atggtcatag gtgttgtcta tctcttacct
     1741 attttgatta atcagttatc tagtttgatt atatctagtc aaactattta tagtcgagta
     1801 caagacttaa tcatagactt atctaattat cctgcgctcc agaatttgga tgtagaagct
     1861 acaattcagc agttaaactt atcctatgtt gatattcttc aaaatatcct aaatagcgta
     1921 tcaaatagtg tggggagcgt cttgtcagct cttatcagta ctgttttgat tttgattatg
     1981 actccagttt ttttggttta tttcttatta gatggacata aattcttgcc catgcttgaa
     2041 agaacgattc taaagaggga tcgcttgcat attgcaggct tattaaagaa tttaaatgcg
     2101 acgattgctc gctatattag tggagtttcg attgacgcaa tcattatagg ttgtttggct
     2161 tatattggct atagtattat tggtttaaaa tatgctttag tttttgccat tttttctggt
     2221 gtagccaatt taattcctta tgtggggcca agtattggtt tgattcctat gatcatcgca
     2281 aatatattca ctgatcccca tagactgctg attgcagtga tttatatgct tgttgttcag
     2341 caggtagatg gcaatatctt atatcctcga atcgtaggaa gtgttatgaa ggttcatcca
     2401 atcacgattt tagttttact tttgttgtca agcaatatct atggtgtagt tggaatgatt
     2461 gtcgcagtgc caacctattc tatcttgaaa gaaatttcta agttcttatc ccgtttgtat
     2521 gaaaatcata aaataatgaa agaacgagaa agagaattag ctaagtaaaa gtcaggagaa
     2581 ccctgatttt tctttactgg aagtggcctt tagattagaa gactgaaaat aagttaaagt
     2641 cttaaactaa ttttcacagc taagaatagt agaagttaat ctgataaaaa tcgaaaaaac
     2701 cagtggaatt ctgtgtcagg gtaagttcca ctggttttca tagtctatta aagttcgaat
     2761 gaaacctatt tatagtagat tgaaactaga atagtacacc tctaattcta aaacattgtt
     2821 agaaatcgat ttgactgtcc tgatctattc gttctattct tatttcattt tactatattt
     2881 tggtgcaata agtgaaaagt agtccgaata atataaggat tgattttata gtttttaaac
     2941 tcaaatgaat tgaaataaag agagtacgaa aattctcatc tgaaagtatt ttagaataat
     3001 tctcttcgtg aatttcttca aaacagatag cttcatctta ggtatgtgat ttctttttgc
     3061 atttttgagt tagataaggt ataatggttt tattgtcttt tggggtcgtt acggattcga
     3121 caggcattat gaggcatatt ttgcgactcg tgtggcgacg taaacgctca gttaaatata
     3181 actgcaaaaa ataacacttc ttacgctcta gctgcctaaa aaccagcagg cgtgacccga
     3241 tttggattgc tcgtgttcaa tgacaggtct tattattagc gagatacgat taagccttgt
     3301 ctagcggttt gataagagat tgatagactc gcagtttcta gacttgagtt atgtgtcgag
     3361 gggctgttaa aataatacat aacctatggt tgtagacaaa tatgttggca ggtgtttgga
     3421 cgtgggttcg actcccaccg gctccattat tcctttgcat tcttttgcat tccttggtaa
     3481 aacgttgtta aatcaacgtt ttttattttt atctttggta ttcctttgca ttcttttgct
     3541 aaaaagggag tcacaaacag accctatttt aaaaaaagga tagaaaaaag gatacaacat
     3601 ttgtcgcatc ctaaaaataa tcttttttcc acggaagaca tgggattcga acccacgcac
     3661 gctattacac gcctaccgcg tttccaacac ggcctcttaa gcctcttgag taatcttcca
     3721 atacttactc aaatagtcta ccataaaggc tcttatcttg caataaaaat tctagaaata
     3781 agaaaaatga tagattttga aagaaaatga taaaaaatgc ttgacttcaa aagaaagtat
     3841 gatagaatga atagtgtaaa cgataacagg aggtgattcg gtgttaaaaa cagagcggaa
     3901 gcaactgatt ttagaggagt taaatcaaca tcatgtagtt tctctagaaa aattagttag
     3961 tttgctagaa acgtcagaat caacggttcg aagagacttg gatgagttgg aagcggaaaa
     4021 caagcttcgt cgtgtgcatg gtggagcaga actcccccac tccttacagg aagaagaaac
     4081 cattcaagaa aaatctgtca aaaaccttca agaaaagaag ttgctggctc agaaagcagc
     4141 ctctctcatt aaagaaaaag atgtcatctt tatcgatgct ggaacaacaa ctgctttttt
     4201 gattcatgaa ttggtcaata agaatattac agttgtgacc aactccattc accatgccgc
     4261 tcagttggtt gaaaagcaga ttccaactgt catggttgga ggaaacgtca agacggcgac
     4321 agatgctagt atcggtggcg ttgctcttaa ccagattaac caattgcact ttgaccgtgc
     4381 ctttatcgga atgaatggtg ttgacgatgg ctattatacg actcctgata tggaggaggg
     4441 agctgtgaaa agagctattt tggagaatgc caagcagacc tacgtcttgg tggattcgtc
     4501 aaaaattgga caaacttgct ttgccaaggt agcaccactc aaacgcgcta tcgttatcac
     4561 tagtcaaggg catgagctct tgcaggttat taaggagaaa acggaggtaa tagaagtatg
     4621 atttatacag tcacactcaa tccatccatt gactatatcg ttcgtttgga ccaagtcaaa
     4681 gttggtagtg taaatcgtat ggacagtgat gataagtttg ctggtgggaa aggaatcaat
     4741 gtcagccgtg tcttgaaacg tttgaatata ccaaatacag cgacgggatt tatcggtggc
     4801 tttactggta aatttatcac agatacttta gcagaggaag aaatcgagac acgttttgtc
     4861 caggtggcag aagatactcg tatcaatgtt aaaatcaaag cagaccaaga aacagaaatc
     4921 aacggaacgg gtccaactgt tgaatcggtt cagctagaag aattgaaagc tattttatct
     4981 agtctgacag cagaagatac agttgtcttt gcaggttcaa gtgctaaaaa tctaggcaat
     5041 gttatctata aggatttgat ttccttgacg cgccagactg gtgcgcaagt ggtctgtgac
     5101 tttgaaggac agaccttaat tgatagtttg gactaccagc ctcttcttgt aaaaccaaac
     5161 aatcatgaac ttggagcgat ttttggggtt aaactcgaaa gtttagatga aattgagaaa
     5221 tacgctcgtg agttactggc taagggtgct caaaatgtta ttatctctat ggctggtgat
     5281 ggtgcccttc ttgtcacatc tgagggagct tacttcgcta aaccaatcaa aggaacagtc
     5341 aaaaattcag ttggagctgg tgattctatg gttgctggat tcacaggtga atttgtcaaa
     5401 tcaaaagacg tagtagaagc cttcaaatgg ggagtggctt gcggaacggc aactaccttc
     5461 tcagatgact tggcaacggc ggaatttatt aaagaaacat atggaaaagt tgaggtagaa
     5521 aaacgatgaa aattcaagac ctattgagaa aagatgtcat gttgctagat ttgcaggcaa
     5581 ctgaaaaaac agctgtcatc gacgagatga ttaaaaattt gacagaccac ggttatgtaa
     5641 cagattttga aacatttaaa gaaggaattt tggcgcgtga agctttgact tctactggtt
     5701 tgggtgatgg aatcgcaatg cctcacagca aaaacgctgc tgtcaaagaa gcgacagttc
     5761 tatttgctaa gtcaaataag ggtgttgact acgagagctt ggatggacaa gcaactgacc
     5821 tcttcttcat gattgcagct ccagaaggtg ccaatgatac tcacttggca gccttggcag
     5881 aattgtctca atacttgatg aaagacggtt ttgcagacaa acttcgtcaa gcaacatctg
     5941 cagaccaagt tatcgaactt tttgaccaag cttcagaaaa aactgaggaa cttgttcaag
     6001 cacctgctaa tgactctggt gactttatcg tagctgttac agcttgtaca acaggtattg
     6061 cccacactta catggcccaa gaagcccttc aaaaagtagc tgctgaaatg ggggttggta
     6121 tcaaggtcga aaccaacggt gctagcggtg ttggaaatca actaactgca gaagatatcc
     6181 gtaaggctaa agctattatc attgcagcag acaaggccgt tgaaatggat cgatttgatg
     6241 gaaaaccatt gatcaatcgt ccagttgctg acggtatccg taagacagaa gagctaatta
     6301 acttggctct ttcaggagat actgaagtct accgtgccgc taatggtgcc aaagctgcaa
     6361 cagcctctaa cgaaaaacaa agccttggtg gtgccttgta caaacacttg atgagtggtg
     6421 tatctcaaat gttaccattc gttatcggtg gtggtatcat gattgccctt gccttcttga
     6481 ttgacggtgc tttgggtgtt ccaaatgaaa accttggcaa tcttggttct taccatgagt
     6541 tagcttctat gttcatgaaa attggtggag ctgcctttgg tttgatgctt ccagtctttg
     6601 cgggttatgt tgcctactct attgctgaaa aaccgggttt ggtagcaggt ttcgtggctg
     6661 gtgctattgc caaagaaggt tttgcctttg gtaaaattcc ttatgccgca ggtggtgaag
     6721 caacttcaac tcttgcaggt gtctcatctg gtttcctagg tgcccttgtt ggtggattta
     6781 tcgcaggtgc cttggttctt gccatcaaga aatacgttaa agttcctcgt tcactcgaag
     6841 gtgctaaatc aatccttcta ttgccacttc ttggaacaat cttgacagga tttgttatgc
     6901 tagctgtgaa tatcccaatg gctgcaatca acactgctat gaatgacttc ctaggcggtc
     6961 ttggaggagg ttcagctgtc cttcttggta tcgtccttgg tggaatgatg gctgttgaca
     7021 tgggtggacc agttaataaa gcagcttatg tctttggtac aggtacgctt gcagcaactg
     7081 tttcttcagg tggttctgta gccatggcag cagttatggc tggaggaatg gtgccaccac
     7141 ttgcaatctt tgtcgcaact cttcttttca aagataaatt tactaaggaa gaacgtaact
     7201 ctggtttgac aaacatcatc atgggcttgt catttatcac tgagggagcg attccatttg
     7261 gtgccgctga cccagctcgt gcgattccaa gcttcatcct tggttcagca gtagcaggtg
     7321 gactcgttgg tcttactggt atcaaactca tggcgccaca cggaggaatc ttcgttatcg
     7381 cccttacttc aaatgctctc ctttacctcg tttctgtctt ggtaggagca atcgtaagtg
     7441 gtgtggttta tggttaccta cgcaaaccac aagcataaaa aatagaaaaa tgaaaagatt
     7501 ggaccgtttg gtgcagtctt tttctcttcc cgaaatgcct gtgaaatatg gtataataga
     7561 agaatggcaa acaagaatac aagtacaaca agacggagac cgtctaaagc agaactggaa
     7621 agaaaagaag cgattcaacg aatgttgatt tcgttaggaa ttgcgatttt attgattttc
     7681 gcagccttca aattaggggc tgcaggtata accctttata atttaattcg cttgctagtg
     7741 ggtagcctag cttatctggc gatattcggc ctattaatct atctcttctt tttcaagtgg
     7801 atacgaaaac aggaaggact cttatctggc tttttcacca tatttgctgg cttactcttg
     7861 atttttgagg cctacttggt ttggaaatat ggtttggaca agtccgttct aaaagggacc
     7921 atggctcagg ttgtgacaga tctgactggt tttcgaacga ctagctttgc tggagggggc
     7981 ttgatcgggg tcgctcttta tattccaaca gcctttctct tttcaaatat cggaacttac
     8041 tttattggtt ctatcttgat tttagtgggt tctctcctag tcagcccttg gtctgtttac
     8101 gatattgctg aatttttcag tagaggcttt gccaaatggt gggaagggca cgagcgtcga
     8161 aaagaggaac gctttgtcaa acaagaagaa aaagctcgcc aaaaggctga gaaagaggct
     8221 agattagaac aagaagagac tgaaaaagcc ttactcgatt tgcctcctgt tgatatggaa
     8281 acgggtgaaa ttctgacaga ggaagctgtt caaaatcttc cacctattcc agaagaaaag
     8341 tgggtggaac cagaaatcat cctgcctcaa gctgaactta aattccctga acaggaagat
     8401 gactcagatg acgaagatgt tcaggtcgat ttttcagcca aagaagccct tgaatacaaa
     8461 cttccaagct tacaactctt tgcaccagat aaaccaaaag atcagtctaa agagaagaaa
     8521 attgtcagag aaaatatcaa aatcttagaa gcaacctttg ctagctttgg tattaaggta
     8581 acagttgaac gggccgaaat tgggccatca gtgaccaagt atgaagtcaa gccggctgtt
     8641 ggtgtaaggg tcaaccgcat ttccaatcta tcagatgacc tcgctctagc cttggctgcc
     8701 aaagatgtcc ggattgaagc accaatccct gggaaatccc taatcggaat tgaagtgccc
     8761 aactccgata ttgccactgt atctttccga gaactatggg aacaatcgca aacgaaagca
     8821 gaaaatttct tggaaattcc tttagggaag gctgttaatg gaaccgcaag agcttttgac
     8881 ctttctaaaa tgccccactt gctagttgca ggttcaacgg gttcagggaa gtcagtagca
     8941 gttaacggca ttattgctag cattctcatg aaggcgagac cagatcaagt taaatttatg
     9001 atggtcgatc ccaagatggt tgagttatct gtttacaatg atattcccca cctcttgatt
     9061 ccagtcgtga ccaatccacg caaagccagc aaggctctgc aaaaggttgt ggatgaaatg
     9121 gaaaaccgtt atgaactctt tgccaaggtg ggagttcgga atattgcagg ttttaatgcc
     9181 aaggtagaag agttcaattc ccagtctgag tacaagcaaa ttccgctacc attcattgtc
     9241 gtgattgtgg atgagttggc tgacctcatg atggtggcca gcaaggaagt ggaagatgct
     9301 atcatccgtc ttgggcagaa ggcgcgtgct gcaggtatcc acatgattct tgcaactcag
     9361 cgtccatctg ttgatgtcat ctctggtttg attaaggcca atgttccatc tcgtgtagca
     9421 tttgcggttt catcaggaac agactcccgt acgattttgg atgaaaatgg agcagaaaaa
     9481 cttcttggtc gaggagacat gctctttaaa ccgattgatg aaaatcatcc agttcgtctc
     9541 caaggctcct ttatctcgga tgacgatgtt gagcgcattg tgaacttcat caagactcag
     9601 gcagatgcag actacgatga gagttttgat ccaggtgagg tttctgaaaa tgaaggagaa
     9661 ttttcggatg gagatgctgg tggtgatccg ctttttgaag aagctaagtc tttggttatc
     9721 gaaacacaga aagccagtgc gtctatgatt cagcgtcgtt tatcagttgg atttaaccgt
     9781 gcgacccgtc tcatggaaga actggagata gcaggtgtca tcggtccagc tgaaggtacc
     9841 aaacctcgaa aagtgttaca acaataaaaa aatagcttct ttccaagttt ggagggaagc
     9901 tattttagtg gctattgatt gcttttattt tctgaagttg gcgcattgga ctgtttttcg
     9961 ttttcagtag caggtttact tgaagcagga gtagaagagt cctgagttgc tgttttctga
    10021 tcttcttttt tctcttcctt gacgctagat tttggtgttt cctcttgctg tgttttttct
    10081 tgactagtgt tagtctcttt agttggactg gtgttttcct taggggattc cttttggatt
    10141 tctttgacaa tggttgtcgt ctggcttgtc gtaggttctt ttttaatatt tttgttatta
    10201 tccaaggcgt tcataatcgt gatacttgcg gtaatcaagc caaggatact agcgattgta
    10261 gcaacggttt tttgaaagac tgtaaagcct tttttgatgt gttctgtttt tggttttgaa
    10321 gttctacgat agttagacat acattctctc ctttgattat gatttattat actctgtttc
    10381 tttaaaaaaa tcttaaaaaa agagaaattt ccctttcttg tcgcaagctc cgtttcatga
    10441 tacaatagag gaagtgattt tagaaagcgt gagaaacatg atttactttg ataattcagc
    10501 gacgaccaag ccttatcctg aagcccttga aacctatatg caggtcgctt caaaaatttt
    10561 aggaaaccca tctagtctcc atcgtttggg agaccaagca acacgaatct tagatgcttc
    10621 tcgccaacag attgcagatt taatcggtaa gaaaagcgat gaaatcttct ttacctcggg
    10681 tggaacagaa ggggataact ggcttatcaa gggtgtggcc tttgaaaaag ctcagtttgg
    10741 caagcacatc attgtttcag ccattgaaca tccagcagtc aaagagtcag ccctctggtt
    10801 gaaaagtcaa ggatttgaag tggattttgc tccagttgat aagaaaggct tggtcgatgt
    10861 tgaggcgtta gcaggtttga tacggcatga tacaatcctc gtttccatca tggctgtgaa
    10921 caatgaaatc ggctctatcc aacctattga ggctatttca gaattcttgg cagacaagcc
    10981 gactatttcc ttccacgttg atgcggttca ggcgcttgcc aaaattccga ctgaaaagta
    11041 tctgacagaa cgggtggatt gcgcgacttt ctctagtcac aagttccacg gggttcgagg
    11101 tgttggcttt gtctatatca aatctggcaa gaagattaca cctcttctta caggtggtgg
    11161 ccaggagcga gattatcgtt cgacaactga aaatgtggca gggattgcag cgacagccaa
    11221 ggccctccgt ttgtctatgg aaaagctaga tatctttagg agcaagactg ggcagatgaa
    11281 ggcagtgatt cgccaagctc ttctgaacta tccggatatt tttgtctttt cagatgagga
    11341 aaactttgca cctcatattc tgacttttgg aatcaaaggt gttcgaggtg aagtcatcgt
    11401 tcacgccttt gaagactatg atattttcat ctcaacaacc tcagcttgtt catctaaggc
    11461 aggaaaacca gccggtacct tgattgccat gggagtggac aaagataagg ccaagtcagc
    11521 tgtgcgtctt agcctagact tggaaaatga tatgagtcag gtcgagcagt ttttgaccaa
    11581 gttaaaattg atttacaatc aaactagaaa agtaagatag gagcattcat gcagtattca
    11641 gaaattatga ttcgctacgg agagttgtca accaagggta aaaaccgtat gcgtttcatc
    11701 aataaacttc gtaataatat ttcggacgtt ttgtctatct atacccaagt taaggtaaca
    11761 gcagatcgcg accgtgccca cgcttacctc aatggagctg attacacagc agttgcagaa
    11821 tctctcaaac aagtttttgg aattcaaaac ttttctcctg tttataaggt tgaaaaatct
    11881 gtagaagttt tgaagtcttc tgtccaagag attatgcggg acatctacaa ggaaggtatg
    11941 acctttaaga tttctagcaa gcgtagcgac cacaactttg aacttgatag tcgtgaactc
    12001 aaccaaacac ttggaggggc tgtattcgaa gccattccaa atgtgcaagt tcaaatgaaa
    12061 agtcctgaca tcaatcttca ggtggagatt cgtgaagaag cagcctatct ttcttatgaa
    12121 accattcgtg gggctggtgg tttgccagtt ggaacttcag gtaaagggat gctcatgttg
    12181 tcaggaggga ttgactcacc tgtagcaggt tatcttgctc ttaagcgtgg ggtggatatc
    12241 gaggcagttc actttgctag tccaccatat actagtcctg gtgccctcaa gaaagcgcag
    12301 gacttgaccc gtaaattgac caagtttggc ggaaatatcc agtttataga ggtgcctttc
    12361 acagagattc aagaggaaat caaagccaaa gcgccagaag cttatttgat gactctaact
    12421 cgtcgcttta tgatgcggat tactgaccgt attcgtgagg tacgaaatgg tttggttatc
    12481 atcaatgggg aaagtctagg tcaagtagcc agccaaaccc ttgaaagtat gaaggctatc
    12541 aatgctgtta ccaacactcc catcattcgt cctgtggtta ccatggacaa gttggaaatc
    12601 attgacatcg cccaggaaat cgataccttt gacatttcaa tccaaccgtt tgaagactgt
    12661 tgtaccattt ttgcaccaga tcgtccaaaa acaaatccta aaattaagaa tgcggagcag
    12721 tacgaagcgc gtatggatgt tgaaggcttg gttgagcgag cagtggctgg aatcatgatt
    12781 actgaaatca cacctcaagc cgaaaaagat gaagttgatg acttgattga caatctgctc
    12841 taattcagaa aatccaaaag aatagcgaaa atcagtaaaa aaagttagtt ttttctctaa
    12901 aaacaggtaa aaaactaact ttttttattt ttatgatata atgatataaa attttgaata
    12961 tagagagttt tctgacaatg aatcaatcct acttttatct aaaaatgaaa gaacacaaac
    13021 tcaaggttcc ttatacaggt aaggagcgcc gtgtacgtat tcttcttcct aaagattatg
    13081 agaaagatac agaccgttcc tatcctgttg tatactttca tgacgggcaa aatgttttta
    13141 atagcaaaga gtctttcatt ggacattcat ggaagattat cccagctatc aaacgaaatc
    13201 cggatatcag tcgcatgatt gtcgttgcta ttgacaatga tggtatgggg cggatgaatg
    13261 agtatgcggc ttggaagttc caagaatctc ctatcccagg gcagcagttt ggtggtaagg
    13321 gtgtggagta tgctgagttt gtcatggagg tggtcaagcc ttttatcgat gagacctatc
    13381 gtacaaaagc agactgccag catacggcta tgattggttc ctcactagga ggcaatatta
    13441 cccagtttat cggtttggaa taccaagacc aaattggttg cttgggcgtt ttttcatctg
    13501 caaactggct ccaccaagaa gcctttaacc gctatttcga gtgccagaaa ctatcgcctg
    13561 accagcgcat cttcatctat gtaggaacag aagaagcaga tgatacagac aagaccttga
    13621 tggatggcaa tatcaaacaa gcctatatcg actcgtcgct ttgctattac catgatttga
    13681 tagcaggggg agtacatctg gataatcttg tgctaaaagt tcagtctggt gccatccata
    13741 gtgaaatccc ttggtcagaa aatctaccag attgtctgag attttttgca gaaaaatggt
    13801 aagttaagaa aggaaaaaac gaaatgcata ttgaacatct tagccactgg agtggtcatc
    13861 ttaaccgtga aatgtacctt aaccgttatg gacatggtgg gattccagtt gtggtctttg
    13921 cttcatcagg tggtagtcac aacgaatact atgattttgg catgattgat gcctgtgctt
    13981 cctttatcga ggaaggcctt gtccagttct ttaccctatc tagtttggat agtgagagct
    14041 ggttggctac ttggaaaaat gctcatgacc aagcggaaat gcaccgtgcc tacgaacgtt
    14101 atgtgattga ggaggccatt cttttatcaa gcacaagaca ggttggtttg atggcatgat
    14161 gacgacaggt tgctctatgg gagcctatca tgcactcaat ttcttcctcc agcatccaga
    14221 tgtctttacc aaagtgattg ctctcagtgg tgtttacgac gcacgtttct ttgtcggtga
    14281 ttactacaac gatgatgcta tttaccaaaa ctcgccagta gattatattt ggaaccaaaa
    14341 cgacggctgg tttattgacc gttaccgtca ggcagagatt gtgctgtgta cggggcttgg
    14401 agcctgggaa caagatggtt tgccatcctt ttacaagctc aaagaagcct ttgacaagaa
    14461 acaaattcca gcctggtttg ctgaatgggg acatgatgtc gcccatgact gggaatggtg
    14521 gcgtaaacaa atgccttatt tcctcggtaa tctctattta taaaaggagt tacctatgaa
    14581 ttaccttgtt atttctccct actatccaca aaactttcaa cagtttacca tcgaactagc
    14641 taataaaggc atcacagtct tgggaattgg tcaagagtct tacgagcaat tggatgagcc
    14701 cttgcgcaat agcttgaccg agtattttcg tgttgataat cttgagaaca tagatgaagt
    14761 caaacgtgca gttgcttttc tcttttataa acatggtcca attggccgca tcgagtctca
    14821 caatgaatac tggcttgagc tagacgcaac actcagagaa caattcaatg tttttggtgc
    14881 caaaccagag gatctcaaaa agacgaaata taagtctgaa atgaagaaac ttttcaaaaa
    14941 agcaggtgtt cctgtggtac ctggagctgt tatcaagacg gaagcagatg ttgatcaagc
    15001 agtgaaagaa atcggtcttc caatgattgc caaacctgat aatggagtgg gagcagccgc
    15061 aacctttaaa cttgagacag aagacgatat caatcacttc aagcaagaat gggaccattc
    15121 aaccctttat ttctttgaaa aatttgtcac ttccagcgaa atctgtacct ttgacgggct
    15181 cgtggacaag gatggaaaga ttgtcttctc aacaaccttt gactacgcct atacaccgct
    15241 tgacctcatg atttataaga tggacaattc ttattatgtg ctcaaggata tggatcctaa
    15301 actgcgcaag tatggggaag caattgtcaa agaatttggt atgaaagaac ggtttttcca
    15361 tattgagttc ttccgtgagg gggacgatta tattaccatc gagtacaata accgccctgc
    15421 aggtggtttt accattgatg tttataactt tgctcattcc ttggaccttt atcgtggcta
    15481 tgcagctatt gtcgcaggag aggagttccc ggcgtcagac tttgaaactc agtattgttt
    15541 ggctacttct cgccgtgcaa atgctcacta tgtttattca gaagaggatt tgcttgccaa
    15601 atatagccag cagttcaagg ttaaaaaagt catgccagct gccttcgcgg aacttcaagg
    15661 agattacctg tatatgctga ccactccgag tcgacaagaa atggagcaga tgattgcaga
    15721 tttcggacaa cgtcaagaat aagaactatc ggattaagga aattaactcc cttaatcctt
    15781 ttgttttgtc tgataaaaaa taagagcatc ccaacaaggt agctatcata aaacttgttc
    15841 gataactatt tgaagcagga ttaggtggtc agaaattaaa ttttaatatt tcaattgagt
    15901 catagtattg tgtttgcgta tccttaaatc agctaaaagg atccatgacg acacctatac
    15961 gatatagttt tcaagatacc aaacaagtct attaatattc aatgaaaatc aaagagcaaa
    16021 ctaggaagct agccgcaggt t
//
LOCUS       DAVBCN010000046        15641 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVBCN010000046 DAVBCN010000000
VERSION     DAVBCN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15641
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.046"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(48..797)
                     /locus_tag="VQ497_001355"
     CDS             complement(48..797)
                     /locus_tag="VQ497_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET2517656.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            930..3266
                     /locus_tag="VQ497_001356"
     CDS             930..3266
                     /locus_tag="VQ497_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET2517657.1"
                     /translation="MDKNKIMGLTQREIKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAVASILTSYMA
                     HSEDKNPTAQAIRQRFQGQVVYPMLSNLPFSSDRKWGAMELEGLGIVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLNY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELIALAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRKSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            3368..4045
                     /locus_tag="VQ497_001357"
     CDS             3368..4045
                     /locus_tag="VQ497_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HET2517658.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            4032..4829
                     /locus_tag="VQ497_001358"
     CDS             4032..4829
                     /locus_tag="VQ497_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HET2517659.1"
                     /translation="MLASEVIHAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            4846..5949
                     /locus_tag="VQ497_001359"
     CDS             4846..5949
                     /locus_tag="VQ497_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET2517660.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTPLASGYQIGEEEFEQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLVQDMEAYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPDLTGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            6250..7269
                     /gene="galE"
                     /locus_tag="VQ497_001360"
     CDS             6250..7269
                     /gene="galE"
                     /locus_tag="VQ497_001360"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET2517661.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTATLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAIEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            7271..8224
                     /locus_tag="VQ497_001361"
     CDS             7271..8224
                     /locus_tag="VQ497_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2517662.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRKLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDDRLKKIEFI"
     gene            8376..9041
                     /locus_tag="VQ497_001362"
     CDS             8376..9041
                     /locus_tag="VQ497_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517663.1"
                     /translation="MLSKEDYTEEIGLIKKQNYVEAELYPIVADIIKPTLKDSLSKRY
                     VFGRQRRGLGQIYYGLSNFPDIVILDKTYENKSRKSIKIEEWKKLRGCVEVKNLNYSL
                     ITEEKIKSTISNSFEHITGEMEQLTGEMGQLIGDLLWYKKVIYTNGIEWRFLSLDDKE
                     EIDNTIVEVVNKRIETEEAGNSFDWWKNIKDLSFNYTDICLSKDCRQEWNEFVKRVKE
                     IEW"
     gene            9080..9181
                     /locus_tag="VQ497_001363"
                     /pseudo
     CDS             9080..9181
                     /locus_tag="VQ497_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group 2 glycosyl transferase"
     gene            complement(9232..9438)
                     /locus_tag="VQ497_001364"
     CDS             complement(9232..9438)
                     /locus_tag="VQ497_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET2517664.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            9490..9966
                     /locus_tag="VQ497_001365"
     CDS             9490..9966
                     /locus_tag="VQ497_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET2517665.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            9975..10646
                     /locus_tag="VQ497_001366"
     CDS             9975..10646
                     /locus_tag="VQ497_001366"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET2517666.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDFETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            10777..11115
                     /locus_tag="VQ497_001367"
     CDS             10777..11115
                     /locus_tag="VQ497_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HET2517667.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLADGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            11118..11747
                     /locus_tag="VQ497_001368"
     CDS             11118..11747
                     /locus_tag="VQ497_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HET2517668.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMIRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            11987..13138
                     /locus_tag="VQ497_001369"
     CDS             11987..13138
                     /locus_tag="VQ497_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2517669.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            13451..14200
                     /gene="truA"
                     /locus_tag="VQ497_001370"
     CDS             13451..14200
                     /gene="truA"
                     /locus_tag="VQ497_001370"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET2517670.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQAI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFTVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            14190..14963
                     /locus_tag="VQ497_001371"
     CDS             14190..14963
                     /locus_tag="VQ497_001371"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET2517671.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            14941..15402
                     /locus_tag="VQ497_001372"
     CDS             14941..15402
                     /locus_tag="VQ497_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2517672.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
BASE COUNT         4473 a         2906 c         3612 g         4650 t
ORIGIN      
        1 tgatatataa atgaaaacct tagctgtttg gccaaggttc atttcttcta tgcaagttct
       61 tctcttttct tatctgggtt ccagatgaag cttgcgataa agctaaagat gatggttagg
      121 atagcgagaa taatagcaag gatgagggca gggatgcgga taaaccacca gagtggattt
      181 ggttttttat cattgtgcca ttgtttcgtt tcttcgtcca gacgttggaa ttctgtttga
      241 atacgattgg cgactgtttc aatgccttca tcattcattt tcttgatatc tgagatatcg
      301 attggatttc caaagttcat atcgacacgt tcacggctaa tcaagccctt caaagtcatg
      361 ggaccggtgt aggtaaccgg catgatacgg accttggcca ttttggcaat cagtgctgcg
      421 ccccccttga catcgtttga gtggcggcta ccacttggaa acatgatgag agagcggtca
      481 ctttttttga gaacgttgat aggatatttg atggctgagg cgctgggatt ttcacggtcg
      541 atgggaaagg cgccacacat acgaatccac caaccaaaga tacggttggt aaagagttct
      601 ttttttgcca taaagatgaa ctgttttggc ttggtcgcaa aggccatata aacaggatcc
      661 caccaggtac ggtgaggcgc aactaaaata taattttcat cttgattagg aattttatca
      721 gtattatgat agtgagcatt gccattgatg gaccataaga gcaatacaac taatccacgc
      781 aaataagtat aaaacatgcg atctccttcg attgttttct tgttattatt ataccttatc
      841 aaaggagggc tggcaaactt ttcccttgac tagatgcata tttgggatga aattagaatt
      901 ctgttaaaaa aaatgatata atagaattta tggataaaaa taagattatg ggattaaccc
      961 aaagagaaat caaggaaaga caggctgagg gtttggtcaa tgactttacc gcatcagcca
     1021 gtaccagcac ttggcaaatc gttaaacgaa atgtctttac cctttttaac gctttgaact
     1081 ttgccattgc tttggccctt gcctttgtgc aggcttggag caatctggtc ttctttgctg
     1141 ttatctgctt taacgctttt tctgggattg tgaccgagct acgagccaaa cacatggtgg
     1201 acaagctcaa tctcatgacc aaggaaaagg tcaaaaccat ccgtgatggt caggaagttg
     1261 ctcttaatcc tgaagaatta gtgctaggag atgtcattcg tttgtctgca ggagagcaga
     1321 ttcctagtga tgccttggtt ttggaaggct ttgcggaagt caatgaagcc atgttaacgg
     1381 gagaaagtga tttggtgcaa aaggaagttg acggcttact tttgtcagga agtttcctag
     1441 ccagtgggtc agttttatct caagttcacc atgtcggtgc agacaactat gctgccaaac
     1501 tcatgcttga ggctaagacc gttaaaccca tcaactcccg tatcatgaaa tcgctggaca
     1561 agttggctgg ttttactggg aagattatca ttccctttgg tctggctctc ttgctggaag
     1621 ccttgctttt aaaaggcctg cctctcaagt catccgttgt aaactcgtcg acagctcttt
     1681 tgggaatgtt gcctaaggga attgcccttt tgaccattac ttcgctcttg actgcagtga
     1741 ttaagttggg cttgaaaaag gtcttggtgc aggagatgta ctctgttgag accttggcgc
     1801 gcgtggatat gctctgtctg gacaagacgg gtaccatcac ccaaggaaag atgcaggtgg
     1861 aggctgttct tccactgacg gaaacttatg gtgaagaggc tgttgccagc atcctgacca
     1921 gctatatggc ccatagtgag gataaaaatc caacagctca agccattcgc cagagattcc
     1981 aaggtcaagt agtctatcct atgctctcta atcttccctt ctccagcgac cgcaagtggg
     2041 gagccatgga gttagaaggt ttgggaatag ttttcttagg ggcacctgag atgttgctgg
     2101 attctgaggt cccagaagcc agagaggcct tggagagagg atcacgtgtc ttggtcttag
     2161 ctctcagtca ggagaaatta gaccatcaca aaccacagaa accatctgat attcaggctc
     2221 tagccttgct ggaaatcttg gatcctattc gagagggagc ggcagagacg ctgaactatc
     2281 tccgttctca ggaagtgggg ctcaagatta tctctggtga caatccagtt acggtatcca
     2341 gcattgccca gaaggctggt tttgcggact atcacagcta tgtcgattgt tcaaaaatca
     2401 cggatgagga attgattgct ctggcggagg agacagcgat tttcggacgt gtttcccctc
     2461 atcaaaagaa actcattatc caaacgctga aaaaagcggg tcatacaaca gctatgactg
     2521 gggatggagt taatgatatc ctggctcttc gtgaggcgga ttgttctatc gtaatggcgg
     2581 agggagaccc ggcaacccgt cagattgcca acctggttct cttgaactca gactttaatg
     2641 atgttcctga gattcttttt gaaggtcgtc gtgtggtcaa taacattgcc cacatcgctc
     2701 cgattttctt gataaagacc atctattcct tcctgttagc agtcatctgt attgccagtg
     2761 ctttactagg tcggtcagag tggattttga ttttcccctt cattccgatc cagattacca
     2821 tgattgacca gtttgtggaa ggtttcccac cattcgttct gacttttgag cgaaatatca
     2881 aacctgttga gccaaatttc ctcagaaaat ccatgcttcg tgccctacca agtgccctca
     2941 tggtcgtgtt tagcgttctt tttgtgaaaa tctttggaac gagtcaaggt tggtctgagt
     3001 tagaaatctc aactctcctc tattatctct tggggtcaat tggtttctta tccgtattta
     3061 gagcctgcat gccattcact ctatggcgag tcctcttgat tgtctggtca gtaggaggct
     3121 tcctagccac agctctcttc ccaagaatcc aaaaactgct tgaaatttca accttgacag
     3181 aacaaacgtt gcctgtttat ggtgtcatga tgttggtctt tacagtgatt ttcatcctga
     3241 ctagtcgcta tcaagctaga aaataaagaa agactgcaat ctgtggattg cagttttttt
     3301 aggtacaaga ttgccagccg aaatgtggta taataagagg taatagagtt ttggaaagtg
     3361 agagaagatg atttcaaaga gattagaatt ggtagcttcc tttgtgtcac agggggctat
     3421 tttactagat gtgggaagtg accatgctta tctgcctatc gagttggttg agagaggcca
     3481 aatcaaaagc gctattgcag gtgaggtggt ggaaggtccc tatcagtctg cggttaaaaa
     3541 tgttgaggct cacggcctaa aggagaaaat ccaagtccgt ttagccaatg gcttggcagc
     3601 ttttgaagag actgaccaag tgtctgtcat taccattgct ggcatgggtg gtcgtttgat
     3661 tgctaggatt ttagaagaag gtttggggaa gttagctaat gtagagcgtt tgatcctcca
     3721 gcccaataat cgtgaagacg acttgcgtat ctggctacag gatcatggat tccagattgt
     3781 agcagaaagc atcttagaag aagctggaaa gttttatgag attttggtgg tggaagcagg
     3841 acaaatgaag ctatcagcca gtgatgttcg ctttggtccc ttcttgtcca aagaagtcag
     3901 tccagtattt gtccaaaaat ggcaaaaaga agctgagaag ctagagttcg ctctcggaca
     3961 aatcccagaa aaaaatctgg aagaacgtca agttctagta gataagattc aagctatcaa
     4021 ggaggtgctc catgttagca agtgaagtga ttcatgctta tgaagccttt tgccctcagg
     4081 aattttctat ggagggagac agccgtggtc tgcaaattgg cactttagac aagggtatcc
     4141 aaagggtcat ggttgccctc gatattcgtg aagagacagt ggcagaggcc attgaaaagg
     4201 gtgtggactt gattatcgtc aagcacgcgc ctatctttcg tcctatcaag gacttgcttg
     4261 ccagccgtcc gcaaaatcag atttacatcg acctgattaa gcatgacatc gcagtttatg
     4321 tcagccatac caatattgat atcgttgaaa atgggctcaa tgactggttt tgtcagatgc
     4381 taggaatcga ggagacgact tatctgcagg aaacaggtcc agaacgtgga attggacgta
     4441 ttgggaatat tcagcctcag acattttggg aattggccca acaggtcaag caagtctttg
     4501 acctagatag ccttcgaatg gtgcattatc aagagaatga tttgcagaag cctatttcaa
     4561 gagtagcaat ttgtggtgga agcgggcagt ctttctataa ggatgctttg gcaaaggggg
     4621 cagatgtcta tatcactggt gatatctact accatactgc tcaggatatg ctgtctgatg
     4681 gcttgttagc attggatcca ggtcactata tcgaagtgct ttttgtggaa aaaatcgcag
     4741 cactccttac tcaatggaaa gagaaaaaag gctgggaact agagatttta cctagtcaag
     4801 catcgaccaa tcctttccgt catatctagt tagaaagtga agacaatgaa aaaagttgcc
     4861 attattggag caggaattgt gggagcaaca gctgcctact acctctcgag agaaagtgac
     4921 ctagaggtga ccgtttttga ccatgagcaa ggtcaagcca ccaaggccgc agcaggaatt
     4981 atcagtcctt ggttttccaa acgccgtaat aaagcctggt acaagatggc gcgcttgggg
     5041 gctgattttt atgtggattt attagctgat ttagagaaat caggacaaaa aatcgacttt
     5101 taccagcgtt cgggagtctt tctcttgaaa aaggatgaat ccaagttgga agaactttat
     5161 caactggccc tccagcgcag agaagaatct cccttgatag ggcaattagc cattctggac
     5221 caagcctcag ctaatgaatt attccctggt ttgcagggat ttgaccgcct gctctatgct
     5281 tctggtggag cgagagtaga tggccaactc ttggtaactc gtctgctaga agccagtcat
     5341 gtcaagctgg tcaaagaaaa agtgactctg acaccgttag catcaggcta ccagattggt
     5401 gaagaggagt ttgagcaggt tattttggcg acgggagctt ggttggggga catgttagaa
     5461 cccttgggtt atgaagtaga tgttcgtcct caaaaaggac aactacgaga ttatcagctg
     5521 gtccaagaca tggaagctta ccctgttgtc atgccagaag gggagtggga tttgattcct
     5581 tttgcaggtg ggaaattgtc cttaggcgct acccacgaaa atgacatggg atttgatttg
     5641 acggtagatg aaaccttgct ccaacaaatg gaggaggcca ccttgactca ctatctgatt
     5701 ttggctgaag ctacttcaaa atctgagcgt gttggaatcc gtgcctacac cagtgatttc
     5761 tctcctttct ttgggcaggt gcctgactta actggtgtct atgcagccag tggactaggt
     5821 tcatcaggcc tcacaactgg tcctattatt ggttaccatt tagctcaact gatccaagac
     5881 aaggagttga ccttggaccc tgaaaactac ccaattgaaa actatgtcaa acgagtaaaa
     5941 agcgaataat actcaatgaa aatcaaagaa caaactagga aactagccgc gggttactca
     6001 aagcactgct ttgaggttgt ggatagaact gacgaagtca gctcaaaaca ctgttttgag
     6061 gttgtagata gaactgacga agtcagtaac catacccatg gcaaggcgac gttgacgtgg
     6121 tttgaattgg attttcgaag agtataagca ttttactgaa attttatcag gtagtttagg
     6181 atggcaaatg acattccctg tcaaaaatgg taaaataaga aaaaataatc tgagaatcga
     6241 ggaaaaaaga tgcaagaaaa gattttggta actggtggtg ccggttttat tggaacccac
     6301 actgttattg aattgatcca agcaggccat caagttgttg tggtggataa tcttgtcaat
     6361 agcaatcgta agagtttaga agttgttgaa agaatcacag gagttgagat tcctttctat
     6421 gaggcagata ttcgtgacac agctaccctc agagatattt tcaagcagga agaaccgact
     6481 ggtgtcattc actttgctgg tttgaaggct gttggcgaat caacacgtat ccctcttgcc
     6541 tactatgaca acaatatcgc tggaactgtc agccttttga aagccataga agaaaacaac
     6601 tgtaaaaaca tcatcttcag ttcttctgcg acagtttacg gggatccgca cacagtgccc
     6661 atcttggaag atttcccact ttcagtgacc aacccatacg gtcgtactaa gctcatgcta
     6721 gaggaaattt tgactgatat ttacaaagca gactcagaat ggaatgttgt cttgcttcgt
     6781 tactttaacc caatcggagc ccatgagagt ggtgatttgg gagaaaatcc aaacggtatt
     6841 ccaaacaatc tcttgccata tgtgactcaa gtagccgttg gaaaattaga gcaagtgcaa
     6901 gtgtttggag acgattacga tacggaagat ggaacaggtg ttcgtgacta tatccacgtt
     6961 gtcgatttgg ctaagggtca cgttgcagct ttgaaaaaaa tccaaaaagg ttcaggacta
     7021 aacgtttata accttggaac tggtaaaggt tactcagttc ttgaaattat ccaaaacatg
     7081 gaaaaagcgg tgggacgtcc tattccttac cgtatcgtag aacgtcgccc aggtgatatc
     7141 gctgcctgct actcagaccc agcaaaagct aaagcagaac tcggttggga agcagaactc
     7201 gacatcaccc aaatgtgtga agacgcatgg cgttggcaaa gcaagcatcc aaatggattt
     7261 gaagactaag atgatgattt caatcatcgt cccttgttta aacgaagagg aagtacttcc
     7321 tcttttttat caggctctgg aagctttact tccagatttg gaaacagaaa tcgagtatgt
     7381 ctttgtcgat gatggatcaa gtgatgggac cttggaactc ttaaaggcct atcgggagca
     7441 aaatccggca gtccattata tttctttctc tcgaaatttt ggcaaagaag cagcccttta
     7501 tgcaggcttg caatatgcga caggagattt ggtggtggtg atggatgcag acctccaaga
     7561 tcctcctagt atgttgtttg agatgaaaaa tgtactagac aaaaatgtag acttggactg
     7621 cgttgggaca cggagaacta gtcgggaggg agaacccttc tttcgcagtt tctgtgctgt
     7681 tctcttttat cgcctcatgc aaaaaatcag cccagtggct ctgccgtcgg gtgtccgtga
     7741 ttttcgtatg atgagaaggt ctgtagtcga tgccatttta agcttgaccg agtccaatcg
     7801 tttttcaaag gggctcttcg cctgggtggg tttcaaaacc tactatttgg actatccaaa
     7861 tgtcgaaagg caggctggca agaccagttg gagttttagg aaactctttt tctactccat
     7921 tgaagggatt attaattttt cagattttcc cttgattata gcctttgtag ctggtctcct
     7981 atcttgtttt ctttctctgc tgatgacctt ttttgttgtg gttcggaccc tcattttggg
     8041 caatccgaca tctggttgga cctctctgat ggctgttatt ctctttcttg gaggcattca
     8101 actcttgacc attgggattc tcggcaagta tatcagtaag atttatttag agactaaaaa
     8161 aagaccactt tatcttatca aagaaaaaag tgatgatagg ctaaaaaaga tagaatttat
     8221 ctgacatagt cactttttgt tatttgtaat tcttttagtt cggagtttgg gtgattttta
     8281 aaagaattag ggatttgaaa tgatttaaaa aatctatgta gactaaattt aaattgtagt
     8341 gacttaataa ataaaaaatt taggagtatt atgttatgct aagtaaagag gattatactg
     8401 aagaaatcgg gctaataaag aaacaaaatt atgtagaggc tgaattgtat cctatagttg
     8461 ctgatataat taagccaact ttaaaagata gtttatcaaa aagatatgtc ttcggaagac
     8521 aaagaagggg tttgggacaa atttattatg gtctaagtaa ttttcctgat atagttattt
     8581 tagataagac ttatgaaaac aaatctagga aaagtataaa aattgaagaa tggaagaaac
     8641 ttcgaggatg cgtggaagtt aagaatttaa attatagttt aattacagag gaaaaaatca
     8701 aatcaaccat atcgaacagt tttgaacata taactggaga gatggaacaa ctaactggag
     8761 agatgggaca acttattgga gaccttttat ggtataaaaa agtaatttat acaaatggaa
     8821 ttgaatggag atttctaagt ttagatgaca aggaagagat agataataca attgttgaag
     8881 tagttaacaa aagaatagaa acagaagaag cgggaaattc ttttgattgg tggaagaata
     8941 ttaaagattt atcgttcaat tatacggata tatgcctatc taaagattgt agacaagagt
     9001 ggaatgaatt tgtaaaaagg gtaaaggaaa tagaatggta gaagtctatt ctcattgaga
     9061 aatttagatt ttaagttaca gtaagattta tttagagact aaaaaaagac cactttatct
     9121 tatcaaagaa aaaagtgacc ttcctgattt tacagaaaaa aataaagtga aaagactata
     9181 attttacatg gaaatgtgct aaactagagg gagtggatta tcgccattga tttaggcttc
     9241 ctggtttcca atcagggcgc gagttgggca atttttaaca gcctctaaga catcctgact
     9301 aggagaaatt tctttttcca gttggtcagg gtcatcgtaa aaacgcacga ttccattatc
     9361 gtggtaatca aataaatcag aataagtttg gcaaagccca caggcgatac atcgttcagg
     9421 tataagtgtg attttcatgt tcatatttat attgtaataa gaaaagtaaa aaaaaacaag
     9481 gagtaaggta tggcaaaaga accgtggcaa gaagatatct atgatcaaga agaatcaaga
     9541 gcagagcgtc ggcatcgaaa ccacggaggg gctgatagga tggctaatcg tattttgacg
     9601 atcctagcta gtattttctt tgtaattgtg gtggtgatgg tcatcgttct catctatcta
     9661 tcatcggggg gaagtaatcg cacagcagcc ttaaaagact ttcatgattc tgatgcaagt
     9721 gtagtacaaa tctcatcttc aagcagttct cagcctgagc agagttcaga gccagaatct
     9781 acttctagta gttcagaaga agctgctaat cctgaaggaa cgattaaagt tctcgcagga
     9841 gaaggggaag cagctattgc cgctcgtgca ggaatctcca ttgctcagtt agaggccttg
     9901 aatcctgggc acatggctac aggatcttgg tttgctaatc caggtgatgt tataaaaata
     9961 aaataggagc cgatatgaaa acaattcaaa ttgctattga tggtcctgct tccagcggta
    10021 agagtacggt cgcaaagatt attgctaagg attttggatt cacctacctt gatacaggag
    10081 ctatgtatcg tgcagcgacc tatatggctc ttaagaacca attaggagtt gaagaagtcg
    10141 aagcccttct agccttgttg gaccagcatc caatcagctt tggacgttca gaaactggag
    10201 accagcttgt ttttgtagga gatgtggata ttacccatcc tatccgtgaa aatgaagtga
    10261 ccaatcatgt ttctgctatt gcagcaattc ctgaagtgcg tgagaaactg gtttctctcc
    10321 aacaagaaat tgcccagcaa ggcgggattg tcatggatgg tcgcgatatt ggaactgttg
    10381 tattgccaca agcagaattg aaaattttcc tagtagcttc tgttgatgag agagcagagc
    10441 gtcgttacaa ggaaaatatt gccaagggaa ttgaaacaga ctttgaaacc ctaaaaaagg
    10501 aaattgctgc gcgtgactac aaggatagtc atcgtgagac ttctcctctc aaacaagcag
    10561 aggatgctgt ctaccttgat acaactggtt tgaacattca agaagtagtt gaaaaaatca
    10621 aagcagaagc tgaaaaaaga atgtaaatgt agaaaagccg atagattgat atcggttttt
    10681 ttctaaattt gtcaaaatgc tgattttagg gtataatagt tgctgttcga gaaattttga
    10741 ttttcaaaga atagtgaatg atgataagga gaaaccatga acaatttacc aaattgtcca
    10801 aaatgtaact cagagtatgt ctacgaagac ggtgccctac tggtttgccc agagtgtgct
    10861 catgagtgga atcctgctga agttgcagaa gtagaagaag gtcttgtcgc tatcgatgcc
    10921 aacggaaata aattggctga tggtgataca gtaactctca tcaaggactt gaaagtaaaa
    10981 ggtgcgccaa aagatttgaa acaagggacg cgcgtgaaaa atatccgcat cgtagaaggc
    11041 gaccacaata tcgactgtaa gattgatggt tttggtgcca tgaaacttaa atcagagttt
    11101 gtgaggaaga tttaagcatg tcaaaacact ataaacttgt attttatagc cgtatcttct
    11161 tgtttctagc ggcttttacg ggagtttatc ttgaaattac taagcatggt ggttttggga
    11221 tgcttctcta ttacacggtt ctgtccaacc tcttggtaac tatttttacc ctttatcttc
    11281 taaaggttat gatccgtgta ggtgaaaatt ggcaaaggcc aagtctcttg cgcttaaaag
    11341 gtggggtcac catgagtatc atgattacct gtgtgattta ccatttcctc ttggcgccca
    11401 ttgcgactaa tttctatacc ctagaaaatt tcctttgcca ctatatcgtt cccatctggt
    11461 ttttagcgga taccctcttt tttgacaaac agggtcaata caagatttgg gatccagcag
    11521 tgtggaccat tttacccttt ctgtatatga tgtttgctct ttttaatggc ttggttctaa
    11581 aactcaatat tccaaatgcc aaggataatc ctttccctta cttctttttg aatgtgaaca
    11641 agggttggaa tgttgtgttt aagtggtgtc tgattatctt tgttgcctat atggtagcag
    11701 gatttatttt ctactttatc aagcaaatca agagaaagtc atcctaatac tcttcgaaaa
    11761 tctcttcaaa ccacgtcagt ttccatctgc aacctcaaaa cactgttttg aacaacctgc
    11821 ggctagcttc ctagtttgct ctttgatttt cattgagtat aagatacaag ggattcattt
    11881 tcgaatttaa gaaggagtct acaaaccaat gaggcttctt cttttcttgc ttatttgata
    11941 actatcaaaa aagtagaaga ttaagagaaa tatagaaaga gatttcatga aacaattttt
    12001 agaacgggcc agcattttgg ctctctccct cgttttgatt acctcctttt ccatttcgag
    12061 tgccctacca gccatgtttg actattatca gggttattct aaggaacaaa ttgagctctt
    12121 ggtgagcttg ccttcctttg gaatcatgat gatgttactg ctaaatggtt tcttagaaaa
    12181 aatatttcct gagcgcttac agattagttt gggcttgctg attttatcat tgagcggtac
    12241 agctcccttc tggtaccaag cctatccctt tgtctttgga acacggcttc tctttggttt
    12301 gggtcttggg atgatcaatg ccaaggccat ttctattatc agtgaacgct accaaggaaa
    12361 aaggcgaatt cagatgttag ggctacgcgc ttctgcagag gtcgttggag cttctctcat
    12421 taccttggcc gtcggtcagt tgttggcctt tggttggaca gctatctttc tagcctatag
    12481 tgctggattt ttggtgctgc ccctttatct gctctttgtc ccttatggaa aatcaaagaa
    12541 agaagtcaag aaaagagcga aggaagcaag tcgtttaact cgagaaatga aaggcttgat
    12601 ttttacctta gctatcgaag cggcagttgt agtttgtacc aatacagcta ttaccatccg
    12661 tattccaagt ttgatggtgg aaagaggatt gggggatgcc cagttatcta gttttgttct
    12721 tagtatcatg cagttgatcg ggattgtggc tggggtgagt ttttctttct tgatttctat
    12781 ctttaaagag aaactgctcc tctggtctgg tattaccttt ggcttggggc aaatcgtgat
    12841 tgccttgtct tcatccttgt gggtggtagt agcaggaagt gttctggctg gatttgccta
    12901 tagtgtagtc ttgacgacgg tctttcaact tgtctctgaa cgaattccag ctaaactcct
    12961 caatcaagca acttcatttg ctgtattagg ctgtagtttc ggagccttta cgaccccatt
    13021 tgttctaggt gcaattggct tactaactca caatgggatg ttggtcttta gtatcttagg
    13081 aggttggttg attgtaatct ctatctttgt catgtaccta cttcagaaga gagcttagga
    13141 ttgattccta agtttttctt ttgatactca atgaaaatca aagagcatag ttgattgagt
    13201 ttggaatagt acgctgtagc tgctaaaata tttctagaaa ttaatttgac ttttctaata
    13261 gagttattca tatcttattt caatctacta tagaaagcta gccgcaggtt gctcaaaata
    13321 gtgttttgag gttgcagatg gaaactgacg tggtttgaag agattttcga agagtatgag
    13381 atttttatac ccatacaata tttgttttag gtccttgttt acagactaat ttcttgataa
    13441 aatagaggtt atgacgagat ataaagcaac tatttcctat gatggttatg cctttgctgg
    13501 ctttcagcgc cagcctcatg cgcgtagcgt tcaggaagaa attgaaaaaa ccttgaccag
    13561 attaaataaa gggcaagcca ttactgttca cggtgctggt aggacagata gtggggttca
    13621 tgccctggga caggtcattc attttgacct gccttatcag atggatgagg agaaactccg
    13681 ttttgccttg gatacccagt ctcctgaaga tattgatgtg atttcgattg agcttgtggc
    13741 agatgatttt cattgccgtt atgccaagca tagcaagacc tatgagttta ctgtggatag
    13801 aggacgtccc aaaaatccga tgcgccgtca ctatgccacc cactttccct acccactcga
    13861 tgtggaacga atgcagattg caatcaaaaa gctagaggga acccatgatt ttaccggttt
    13921 tacagcctct gggactagtg tagaggataa ggttcgcacc atcacagaag ctagtttaat
    13981 agtcgatgag acaggacaat ttttgacctt taccttttca ggaaatggtt tcttgtataa
    14041 acagattcgc aatatggtgg ggactcttct caaaatcggt aacaaccgca tgccagtaga
    14101 gcagattgat ctcatcttgg agaagaagga caggcaactt gcaggtccca ctgcagcacc
    14161 aaatggtttg tatttaaagg agattcgtta tgaagaataa tcgtatttta gcactttctg
    14221 gaaatgatat ttttagtggt ggtggactgt cagctgattt ggctacctat accttgaacg
    14281 gtttgcatgg gtttgtagca gtgacttgtt tgacagcctt gacagaaaaa ggatttgaag
    14341 tctttccaac tgatgatacc atttttcaac atgaattaga tagcttgcgt gatgtggaat
    14401 ttgggggaat taagattggt cttctcccta ctgtcagtgt ggctgagaag gccttggact
    14461 ttatcaaaca acgcccagga gtacctgtgg tgttggatcc tgtcttggtc tgcaaggaaa
    14521 cgcatgatgt agctgtcagt gagctctgcc aagagttgat tcgctttttc ccttatgtca
    14581 gtgtgattac gcctaatctc ccagaagcag aattattatc cggtcaggaa attaaaacct
    14641 tggaagacat gaaaactgca gcgcagaaat tgcatgattt aggagcgcca gcagtcatta
    14701 tcaagggagg caatcgtctt agtcaggaca aggctgtgga tgtcttttat gatggacaga
    14761 cctttactat cctagaaaat ccagttatcc aaggccaaaa tgctggtgca ggttgtacct
    14821 ttgcctctag cattgccagt cacttgatta aaggtgataa acttttgcca gcagtagaaa
    14881 gctctaaggc tttcgtttat cgtgctattg cacaagcaga tcagtatgga gtaagacaat
    14941 atgaagcaaa caaaaacaac taaaatcgcc cttgtatccc tattaaccgc cctttctgtg
    15001 gttctaggtt atttcttaaa aatcccaaca cctacaggaa ttctaactct tttagatgct
    15061 ggtgtcttct ttgcggcctt ttactttggt agtcgtgaag gagcggtagt cggaggacta
    15121 gcaagtttct tgattgacct cttatcaggc taccctcagt ggatgttctt tagcttggtc
    15181 aaccatggct tgcagggatt tttcgcagga tttaaaggaa aaagtcagtg gttaggcctt
    15241 attttagcaa ctattgccat ggtaggaggc tacgccttgg gttctacttt gatgaatggc
    15301 tgggcagcag ccctcccaga aattctacca aatttcatgc aaaatatggt agggatgatt
    15361 gtaggattta ttcttagtca aagtatcaag aagattaagt aatactcttc gaaaatctct
    15421 tcaaaccacg tcagcttcca tctgcaacct caaaacactg ttttgagcag cctgaggcta
    15481 gctttctagt ttgcactttg attttcattg agtataaaga ggttgagtaa aaagtatttc
    15541 aaataagaaa aacgcatagt atcaggtgtt gaatgaactg ccccccaaaa agttagacag
    15601 aaaaaatcta actttttggg gtgtttttat tatgaaatta a
//
LOCUS       DAVBCN010000047        15613 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVBCN010000047 DAVBCN010000000
VERSION     DAVBCN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15613
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.047"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(1..741)
                     /locus_tag="VQ497_001373"
                     /pseudo
     CDS             complement(1..741)
                     /locus_tag="VQ497_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567576.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
     gene            complement(879..2501)
                     /gene="groL"
                     /locus_tag="VQ497_001374"
     CDS             complement(879..2501)
                     /gene="groL"
                     /locus_tag="VQ497_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET2517673.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(2517..2801)
                     /gene="groES"
                     /locus_tag="VQ497_001375"
     CDS             complement(2517..2801)
                     /gene="groES"
                     /locus_tag="VQ497_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET2517674.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(3071..3466)
                     /locus_tag="VQ497_001376"
     CDS             complement(3071..3466)
                     /locus_tag="VQ497_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET2517675.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(3544..4305)
                     /locus_tag="VQ497_001377"
     CDS             complement(3544..4305)
                     /locus_tag="VQ497_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET2517676.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(4338..4964)
                     /locus_tag="VQ497_001378"
     CDS             complement(4338..4964)
                     /locus_tag="VQ497_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HET2517677.1"
                     /translation="MIFTYNKEHVSDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(4980..5297)
                     /locus_tag="VQ497_001379"
     CDS             complement(4980..5297)
                     /locus_tag="VQ497_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET2517678.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(5294..5593)
                     /locus_tag="VQ497_001380"
     CDS             complement(5294..5593)
                     /locus_tag="VQ497_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HET2517679.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(5878..6069)
                     /locus_tag="VQ497_001381"
     CDS             complement(5878..6069)
                     /locus_tag="VQ497_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HET2517680.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFSFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(6230..6904)
                     /locus_tag="VQ497_001382"
     CDS             complement(6230..6904)
                     /locus_tag="VQ497_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517681.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYAK"
     gene            complement(6910..7356)
                     /locus_tag="VQ497_001383"
     CDS             complement(6910..7356)
                     /locus_tag="VQ497_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2517682.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(7470..7973)
                     /locus_tag="VQ497_001384"
     CDS             complement(7470..7973)
                     /locus_tag="VQ497_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET2517683.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(7970..8332)
                     /locus_tag="VQ497_001385"
     CDS             complement(7970..8332)
                     /locus_tag="VQ497_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HET2517684.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(8398..10257)
                     /locus_tag="VQ497_001386"
                     /pseudo
     CDS             complement(8398..10257)
                     /locus_tag="VQ497_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(10296..10505)
                     /locus_tag="VQ497_001387"
     CDS             complement(10296..10505)
                     /locus_tag="VQ497_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2517685.1"
                     /translation="MELEASPVDKQAKFVRLTDKARSQMQQVKAFFERIDKQLMEDID
                     EDELLIFEKVLGQLQAKYQGNRRRE"
     gene            complement(10515..10745)
                     /locus_tag="VQ497_001388"
     CDS             complement(10515..10745)
                     /locus_tag="VQ497_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2517686.1"
                     /translation="MDKPMLVFKRFGHQTYLMVQKEAKRCGIEFMGGPQGQVVRFWDN
                     REKNQDLVLIKDIEQELKITKSVASNLVKRIV"
     gene            complement(10869..11585)
                     /locus_tag="VQ497_001389"
     CDS             complement(10869..11585)
                     /locus_tag="VQ497_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2517687.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHVIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(12466..13881)
                     /gene="ply"
                     /locus_tag="VQ497_001390"
     CDS             complement(12466..13881)
                     /gene="ply"
                     /locus_tag="VQ497_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HET2517688.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(13892..14224)
                     /locus_tag="VQ497_001391"
     CDS             complement(13892..14224)
                     /locus_tag="VQ497_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517689.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(14295..14903)
                     /locus_tag="VQ497_001392"
     CDS             complement(14295..14903)
                     /locus_tag="VQ497_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517690.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(14916..15374)
                     /locus_tag="VQ497_001393"
     CDS             complement(14916..15374)
                     /locus_tag="VQ497_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HET2517691.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            15499..>15613
                     /locus_tag="VQ497_001394"
     CDS             15499..>15613
                     /locus_tag="VQ497_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET2517692.1"
                     /translation="MNYEASKQLTDTRFKLLVGVQRTTFKEMLAGLKTAYQK"
BASE COUNT         4502 a         3600 c         2858 g         4653 t
ORIGIN      
        1 ttgatatgag aatagtcttt ctcaacatcc ttatccaaca agcatgctgt ggcgtccttc
       61 ttaacttgaa ccaggtcaat attctgggct tgggctgcca tagacgcagc cacaataaca
      121 ttgacgatag atatcatact cctcacacat ctctactgaa cgtttgtagc cttgattttt
      181 cttgaaatcg ttgggaagat agtgggtcgt gtaaattttt tgcacatcga ttccgatgct
      241 attgatggtt tgagaattct tatgaggact gatggtcaag gctgaaccaa agtagtcaaa
      301 gcccaagtcc atagccactt gcgctgtttt atccagtcgg tagtcaaaac aaaccttgca
      361 acggtcgcca ccttcgggct cctcctctag tcccctaact agttttcggt attaattggg
      421 ttcgtaggga gcttctaggt actgaaccgt atttcctgtc cgatcattaa aatcactaac
      481 aaatttcttg atgacataga cccgcttatg gtattctact ttgggatgga tattagaatt
      541 ggcaaaatag atggtcacat ctgcatactt ggtcaaatat tctagtgtat aggtactaca
      601 aggggcacag caaacatgca cgagaatggt tggccgctgc tcatttttct cccatgcttg
      661 taccattttc tgcatgacac ggtcataatt aatcttctga ttggggggca tcttgcacag
      721 aatttcttct acatcgatca tgttcttctc ctttttctag tcttatttta tcatataagt
      781 tagaagagct caaatcaatt tagaagtgaa tattataaaa agaagggtta gtcattccct
      841 ccttttgtgt tttttataag ttgttttcta tagaaagctt acatcatccc gcccatcata
      901 cttggatcca ttgctggagc tggggctact ggttctggtt tattggctac gactgcttct
      961 gttgtcaaaa tcaagctggc tacagatgct gcattttgta gggctgaacg actcacttta
     1021 actggatcaa tgataccttg atcaatcatg ttaacccact cgccagttgc tgcgttgaat
     1081 cctataccaa gctcagcatt tttcaaacga tcgataacga tagatccttc aaatcctgca
     1141 ttgtgagcaa tttgacgaac gggttcttcc aaagcacgga gaacaatatt acgtcctgtt
     1201 gcttcatctc ctgtcaattc caaggtagca acagctggaa tcacattggc aagagctgtt
     1261 ccaccacctg caacaatacc ttcttcaaca gctgcacgag tagcgttgag ggcatcttca
     1321 atgcggagtt tcatttcttt caactcagtt tcagttgcgg ctccgacctt aataaccgct
     1381 acaccacctg acaatttggc caagcgttct tgcaattttt cacggtcaaa ttcagaagtt
     1441 gtagtttcga tttgagactt gataaccgca acacggtgag aaatcgcttc aggatttcct
     1501 gcaccttcta caataaccgt gctatctttg tccacggtca ctctcgctgc ttgaccaaga
     1561 gcttcaattg tcgcatcttt caactcaaga ccaaggtctt ctgtgataac tgttccgcct
     1621 gttaagatgg cgatatcttc aagcatggct ttgcgacggt caccaaaacc aggtgccttg
     1681 actgctacta cgttgaaggt tccacgaatc ttgttcaaaa caagagttgg aagagcctcg
     1741 ccatccacat catccgcaat aatcaagagt ggacgattgc tttggagaat gctttccaaa
     1801 agtggcaaga tttcttggat attggaaatt ttcttgtctg taatcaaaat gtacggattt
     1861 tcaaggtcag ccaccatttt ttcgctatca gtcaccatgt actgtgaaag gtaaccacgg
     1921 tcaaactgca ttccttctac gacttcaagc tctgtttcca taccacgtga ctcttcgatg
     1981 gtgatgacac cgtctttgcc aactttttcc attgcttcag agatgtactc accaactttt
     2041 tcagaacgag aagatacggc tgcaacttga gcgatagctt ctttattggc aacagggatg
     2101 gcgttgtttt tcaaagcttc aactgctgcg gcaactgctg tttcaatccc acgacgaata
     2161 ccgattggat ttgcacctgc tgtgacgttt ttgattcctt cacggacgat tgcttgggtc
     2221 aaaacagttg cagttgtagt tccatcacct gcgatatcat tggtttttga agccacttct
     2281 gataccaatt tggcacccat attttcaaaa tggtcttcta attcaatttc tttggcaata
     2341 gtcacaccgt cattggtaat caatggtgag ccaaatgatt tttcaagaac gacattgcga
     2401 ccttttggtc ccaaggttac tttaacagtg tctgcaagga tatcgacacc acggaccata
     2461 gctgaacggg catctgatga aaatttaatt tcttttgaca tacttacttt ctccttctat
     2521 tcttcaatga ttgccaagat gttagcttcg cctacgatga tgtacttttc atcgccatct
     2581 ttgacatcaa gacctgcgtg ggcttcaact aagacacggt ctccagtttt aacacttgga
     2641 gcaaccaagt caccgttcaa ggtacgaaca ccttgtccag tagccacaac ttgggctgtt
     2701 ttggtttttt cttgggctga acctgcgaga acaaagcccc caacggtttg ttctttttct
     2761 tctattttta agaccacacg gtcccctaat ggtttcaaca tctgatttcc tccataatga
     2821 gatagatatt attagcgctc tttatacctg agtgctaatt catagttcta ttgtatcact
     2881 tagtcagaaa taatcaagaa aaaagtctga ctttctcaag ataaaaagcc tgagaccaac
     2941 tcagactttt taatgtttat actcaatgaa aatcaaagag caaactagga agctagccgc
     3001 aagctgctca aagcattgct ttgatgttgt ggataaagct aacgtggttt gaagagattt
     3061 tcgaagagta ttaaaatggc aattcttcct cttccaagac taaatctgcc aaatcttggc
     3121 ctgcattatt ttcacgcatg gcacgttgag cacgactttc caagagttgg aatcctgtta
     3181 caagtacttc ggtcacgtag ttcatttggc catttttctc aaagcgacgg gtacgcaatt
     3241 ctccatcaac ggaaatgaga ctacctttgg ttgcgtagct tgccaaagtt tctgctagtc
     3301 tgccccatag gaccatattg acaaaatcag cttcacgttc accgttttgg tctttgtaac
     3361 gacggttcac agcgatagtt gctcgcgcta ccgacttgtc attgttggtt ttgtgcaatt
     3421 ctggtgtaga cgttaaacgc ccaatcatga taactttatt atacatattt tcttcctcct
     3481 acttatctat tcgtaggaaa tcaaaaaaag ttacagaaat ttgtaacttt tcgaggaaat
     3541 ttcttatttt ttatgaatca tgaaacctgt cgcctgttga ttggccataa tggtcatatc
     3601 tgtaatctga acacggcgag gctgactggt cacatagact actgtgtctg caatatcctg
     3661 agcttgcaag gcttctattc cttggtaaac ggacgcagct cgctctttat caccatgaaa
     3721 acgaacagtt gagaaatctg tttcgacaat cccaggctga atggttgtca ccttgatatc
     3781 cgttgcgatg gtatcaattc gcagtccatc cgaaaaggtc ttaaccgcag ccttggtagc
     3841 tgagtaaaca gcggcaccag cataggcgta gattcctgcg gttgacccca tattgataat
     3901 atgaccttga ttgactttta tcattgctgg caagaaacag cgagtaaccg ccatcagacc
     3961 tttaacattg gtatccaaca tggtcaacat atccaactcc tcatagtctt gatagggagc
     4021 caagtcaaga gccagtccgg cgttgttgac caagatatca atcctcccta tcgtttctag
     4081 aatatcagag caaacagtct tcaccatgac catatccgtc acatctagtg gaaaagtcca
     4141 gacggtttga tttggaaagc ttactgcaaa ctccgacttg aggatttcta atctgtctat
     4201 ccgtcgtcct gttagaacga catcctcacc ctgctccaga taagcacgcg caatcgcttc
     4261 accgattcct gaggttgctc ccgtaatcac tacatttttt gccatcttat ttccttctat
     4321 cagatgctga caagttccta ggcagtccag tgtttcgctg ggtcgaacgg tgttccgaca
     4381 acttggtctt ctgataattc aagcacccca cgtttttgcg gagcatttgg caaatgcaat
     4441 tcacgaggac tacacatcat cccaaaactc ttttcaccac gaagttcgcc tgggaaaatg
     4501 aaattccctt ttggcatcat ggcaccagga agagccacaa tggttttcaa cccaacacgc
     4561 gcattaggtg ctcctgcaac gatttgcact atcttgtcac ttgcgactgc aacttggcag
     4621 atgttgaggt ggtcactgtc tggatgagct accatctcga caatttcacc aacaacaaac
     4681 ttaggttcct tatcattaac aatttcttct gtaaaacctt ccgcctgtaa ttcttggttc
     4741 aaacgagcga cttgctcatc tgataaaaag acttgaccgc gctctgcaat ttcaaacaag
     4801 ctcgaaacct caaaaatgtt ccaagccact gtttccccat tttctttgag gaaaacacgg
     4861 gctaccttgc ctttgcgttc cacattcagt ttggcatctc cgctattttt cacgatgacc
     4921 ataaggacat cactgacatg ttctttgtta tacgtaaaaa tcattgtttc ctttttctcc
     4981 tatttcaatc ctgctaaaaa atcgttaatt tgctccttac ttttacggtc gcgattgaca
     5041 aaacgaccga tttccttgtc cttttctaga acaacaaggc taggaattcc gtaaacatcc
     5101 cagagtttgg ccaaatccat atactgatct cggtccattc gaataaaggt gaactctgga
     5161 ttggtctctt caatctctgg taaggcagga taaatataac gacaatcgcc acaccagtct
     5221 gccacaaaaa ggaagacctt cttgcccgct ttttccacta aagatgctaa ttcttctaaa
     5281 cttgctggct gtatcataag acttcctcct catagactag gtcttcattt tcatagacaa
     5341 aggtataatg acggccatcc tcaaaaatga cgccaccaac caagctctcc agactgcttt
     5401 cgtaaacttg aacataaagg gtcgcaattt cccccatgtc ggaaaaatgg tctcgcacaa
     5461 tctctgtcaa ctcttcctga gtcttcatga gcttacggtc atctgcaact tttttcgtag
     5521 caagagcaag gcttccgata cctagcagag ccaagcctgc catccacatt tttttagctt
     5581 tcataccatt cattttaaca caaaaaaggc ttcaggacaa atgaggaagc agcagaaaag
     5641 caagtaaaaa gcctcttcct ttaaggaaaa ggacttctta tactaagagc aaactagaaa
     5701 gctagccgca ggctgctcaa aacacggttt tgaggttgta gataaggcga cgttgacgtg
     5761 gtttgaagag attttcaaag agtattattc ttattgccag gcacctaagt tgccaacgta
     5821 gtaaccatca ggtgtgtagg tattgcgaag catcttacct gatgaagcca gataatacta
     5881 cttgccattg tctttgaccc aatcattcgc aatcatggaa ccagaagaac ttacataata
     5941 ccattctccc ttgtcataaa cccaagtact gactttcatg gttcctgagc aattaaagga
     6001 aaaaaaactg tccaatatca ttcgtttttt aaaagcattc gacactacat catctacgct
     6061 gtagtacata gatacatcaa acgtaaaagc caaaatatcg aaaaatatac aacttttaaa
     6121 ggcagcattt cattttttaa agtctaacaa ccatagaact gaaatcagag caaggcatca
     6181 atttcctact cttagtacgc ctattttgat ggttgaagtg aagatttttt tatttagcgt
     6241 agacaatttc caacttccca aaagccacat ctccacggat aatcaaggtt ttgctggttg
     6301 gggctacagg agcgtcagcc ttagctgcac caaaggaggt ttctactttc aaatctacac
     6361 gccagtgttg tggaacatag acggttgcat tcccgaaggc cacttcaata tttaaagttg
     6421 caaaatcacc tagcatctct gcattatcat agtagatttt agcatcccca aaagcgactt
     6481 ccacttggtc atctacgaga tcttgatctt gcttatagaa ggtcccgcta ccaaaagcga
     6541 cttccttatc cgtgacgact gtttttttcc cattgtacca ccattttttt tcattccaga
     6601 acttacttga atgcgtcaga taaccaacac caagtaccac caagatgcta gcccaaataa
     6661 gagaatgatt ggtaactggt aacaagtcat aagcgtaatt tgcaatgatg agcgccagta
     6721 aacctgtaaa aactgccgaa gtgagatgac gtctaaggat ggactcaatg gacttataag
     6781 caaaaaaaac aatacctagt aaaggccata ttttaccatc caaagaagga attccaaaat
     6841 tcccttgcag caagatccaa gctgctaaaa ccaataaaac aataccaaat gctttctttt
     6901 tcatgttttt tacctcatgt ttcttagatt ttcttttagg agggattggt aatgccgtga
     6961 gacatgaacc tccttgtgcg tctgataaaa ggaaatggtg cccgttcctg aaaaggactt
     7021 gtccactgag taaatctgac ggatgtttgc gatcgttgac ttggaaactc gattaaaata
     7081 gcgaggcaag atagactcca actcatagag cttgaggcga acctcataag cttccttctg
     7141 gttatgagcg tagatcttgc tcccttctgt ttcaaagaag agaatttctg acaggtctag
     7201 ataatattca cctgtcccct tgtaaaaagt caacctcgga gcctttgact cttgcaagag
     7261 ccgttgcaag tctgcaattt catctgtcaa agtgggggtc ttgatgacaa tttcagtctc
     7321 ctctaaattg ccgtcaatct caattcgtaa cttcatcaca tctcctttct gactctacta
     7381 tatcaaagct aagttaggaa tacaagggca aaaaagcaag tggtcaattt gaagcagtaa
     7441 gtggttgtag gactagctta acttacatgt cagaatagga agaaaaggaa tccccacacc
     7501 aaaccacaag catagccaac caagacatcc ttgggataat gaacaccccc taaaaccctc
     7561 accaacccca gcaaagctga aaagatcaag catactaagc caataggtag actagcatgc
     7621 agacaggcca tggagatgat agttgccgaa aagacatggc gactgggcat cgaccgtccc
     7681 gaactatcct tatcaagaag gggacagata tcccaagttt cataaggtct cgggtgattg
     7741 attttcttac gaaacaggga caaggccaca aaacctgatg caggaataag cacataaacc
     7801 ccaacttgct gaccaagtcc tagttgcagg taagtggtga ctagcaagat cagatagacc
     7861 aggggcatgg cgactgtcat caaacgattg aaactgcgta ataggaataa aagaattgga
     7921 cgactagtta gtctggagct gatatttcga taccattctt gataattttt catttatgct
     7981 tctcatactt ttctagaact tgttgagatg ttttcccctt ggcaagccaa tccactagct
     8041 tgtccaagcg acgtatttcc tgcatcagag gatcatcaat ttcctcaata cggacaccgc
     8101 aaatcttccc tgtaattgca attcgttcag ggacataata tggtgcttgt ctaaaaaaat
     8161 ctccgtaagt aacatcccta tccagacaag caagaacatc actcacttca taccctgtca
     8221 accaactggt cacttggtga actgactctg cctttccacc ctttcgctca acttttgcaa
     8281 tcaaggctaa gtagacagca gcaaatttca tattgtataa tttctgactc atacttctct
     8341 ctttctatgt gtactagtat aaataagaaa aagaaggccg tcaagccttc ttttgattta
     8401 ttcttctgct tcatcttctg taaattgact attgtacaag tcagcgtaga agctaccttg
     8461 cgccatcagt tcctcatagt tgccttgctc gatgatattt ccatctttca tgaccaagat
     8521 caagtctgca tttcggatgg ttgacaagcg gtgggcaatg acaaaggatg tgcgtccttc
     8581 catcaaacgg tccatggctt tttggatcaa ttcctctgtc cgtgtgtcaa cagaagaagt
     8641 cgcctcatcc aaaatcaaaa gcggtgcatc cttaagaagg gcacgagcaa tagtcaatag
     8701 ttgtttttgt cctacagaca aggtcacggt gtcatccaag atggtatcat agccatctgg
     8761 caaggtcata ataaagtggt gaattcccac agccttacta gcttccatca ttcgttcatc
     8821 actaatccct atttgattat agatgagatt gtctcgaata gttccttcaa agagccaggt
     8881 atcctgcaag accattgaaa aggcatcatg cacttctgaa cgcgtcatag ccttggtatc
     8941 cacaccatca atgtgaatac ttcccttatc aatctcatag aatttcatca aaagattgac
     9001 aatggttgtc ttaccagtcc cagtcggccc aacaatggca accttttgac cagcatgagc
     9061 tgtcgcagag aagtcatagt cttgaacatt gacaccgtcc accagaattt ctcctgctga
     9121 cacgtcgtag aaacgtggaa tcagattgac cagagttgat ttaccagaac ctgttgaccc
     9181 aataaaggcc actgtttgac cagtttctgc tttaaagcta acatgttcaa taactgcctc
     9241 cgaatttgcc gcatagcgga aggtcacatc cttaaactcg acctgacctt tgaagttttc
     9301 atcagtcagc tgcacttgaa cagggttttg gatagaagaa tgcaaatata aaacttgatt
     9361 aatccgctta gcagagacca tagttcgggg aagaacgatg aagagtgctc ccatgagaag
     9421 gaagcccatg acaacctaca tggcataaga catgaaaaca atcatgtcac taaagagagg
     9481 cagacgcgct atcggagcag cgtcgttaat cacataggcc ccaatccagt aaatcgccac
     9541 actcaaacca cttgaaatcc ccatcatgat aggattcaaa atagccataa gacggttgac
     9601 aaacaaattc aaacgggtca attcatcatt tactgctgca aatttttcat tttgataatc
     9661 ctctgcattg taggcacgaa cgacacgaat acctgttaaa ctctcacgag tgatactgtt
     9721 cagtttatct gtcagcccct gaatcaagga ctgttttgga aaggctagcg tcatcaaaac
     9781 ggtcgtcatc aggacgttga caatcactgc cacaagtacg gcccagagcc agtattctga
     9841 atgacctaaa atcttcccaa tagcccagat agccataatt ggaccacgcg ttaccacttg
     9901 caagcccata gtaatcaaca tttgaacttg agtaatgtca ttggtagtac gcgtcaagag
     9961 gctaggaatt gaaaatttct taatctctgt ctgcgagtaa tccaaaactc ggttaaaaat
    10021 atcacttctc agcctactaa tataaaaagc cgccactcgg gatgcaaaaa atccaactgc
    10081 aactacggac aagaaggcaa gaaagggcat tcccatcatc atgcttgccg gctgccacaa
    10141 ctcatctaaa ttagttcctt gactacctag caaatccgta attttcgaga tataggtcgg
    10201 cacttccaac tctagataga ccgaaaagca agtaaagaga atggctagta aaatcatccc
    10261 ccattctttt ctactaattc ttttggctaa tttctttatt ctctcctcct attcccttga
    10321 tattttgcct gtagttgacc gagaactttc tcaaaaatca gtaattcatc ttcatcaatg
    10381 tcttccatca actgcttgtc tatgcgttca aaaaaagcct taacctgttg catctgagaa
    10441 cgtgctttgt ccgtcagacg aacaaactta gcctgcttat caacaggact cgcctccaat
    10501 tccaccaaac cattttacac tatacgctta accagattac tagcaacaga cttggtaatt
    10561 ttgagttcct gctcgatatc tttaatcaag accaagtctt ggtttttctc gcgattatcc
    10621 caaaaacgca caacctgacc ttgcggccca cccataaatt caatgccgca acgtttggct
    10681 tccttttgca ccatcaggta agtttgatga ccaaaacgct taaagactaa catcggttta
    10741 tccataatct cccccttcta aataaaaata gttctctgga gaataattaa atttctatga
    10801 gaactatttt cttgattaaa aaaatcccaa gtgattttct cacttaggat catgttctat
    10861 aggttaaatt aaaacccatc tacgttcgta taaatctttt ggacgtcttc gtcgtcttca
    10921 agaacgctgt aaagtttttc aaaagtttca aggtcttcgc ctgacaattc cacttctgac
    10981 tgaggaatca tttccaattc agtcacttgg aattcttcaa taccagactc acggagggca
    11041 acgatagcct tgtgaaggtc agttggcgct gtgtaaactg tgattgtacc ttcttgtgct
    11101 tctacgtcat ccacatccac atccgcttcg agcaattgct caaagactgc gtctgcatct
    11161 tcacctccaa atacaataac acctttgttg tcaaagaggt aagaaacaga acctgaagcg
    11221 cccatgtttc cgccgttttt accaaaggct gcacggacat tggctgctgt acggttaacg
    11281 ttagaagtca aagtatccac aattagcata gagccatttg gcccaaaacc ttcgtaacgt
    11341 ccttctgtaa aggtttcgtc tgtgtttcct ttggctttat caatcgcttt atcgataacg
    11401 tgttttggca cttgggcttg tttagcacgg tcgataacga atttcaaagc tgagtttgat
    11461 tctggatctg gatcaccttt tttagctgct acatagattt ctacaccaaa ttttgcatat
    11521 actttagagt tggctccatc tttagccgtt ttcttggcta cgatattggc ccatttacgt
    11581 cccattagga atctcctttt ttcacatttt aatctttctt attataacac aagttttttt
    11641 gattttcact agaggaaatg gattttatta gcaaatcaag ctaggatagt actttacctg
    11701 ctaagatggt cttgcctttc tatctttatc aacagacact catccacatt caaaaaacaa
    11761 actagaccat tatctgcaaa tagaaagttt cagccaagtt tgacaaagtc agctcaaatt
    11821 actgtttgaa gtttgtagat ataagcgaca aaaacaatca tactgcacct tttgttgaca
    11881 gtctactcca gacatatcat agttccagta aatactttgc aatccaacag ttcttatagg
    11941 cgctattgta ttctaagaaa ttaatagacg agtttctaag caaacctcta atactcaata
    12001 aaaatcaaag agcaaactag aaagctagcc gcaggttgct caaaacactg ttttgaggtt
    12061 gcggataggg ctgacctggt ttgaagagat tttcgaagag tataatttac gtgttcccaa
    12121 gatggagaag ttagactagt acactggcac ttctaaacca ttgctagcaa ttgatttgtt
    12181 catatttaat ttcatttctt ccataaatgg gtattagata taaacagcaa aatatttccg
    12241 atacgtgtcg ttcttgaatt tccaatcatc taaaacaagt aaaggataat caatcccctg
    12301 tatatcaagg aattggctac cctttttact tttttacaca ttctgtttga tagattcatt
    12361 ttaacatcac gagcatactc caatggaaat cgctaggcaa gagataaact ttcagatatc
    12421 cgcagagaga tcatcgcctc tttttgtcgc aagcattctc ctctcctagt cattttctac
    12481 cttatcttct acctgaggat agagagttgt tccccaaata gaaatcgtcc gcttacgcac
    12541 tagtggcaaa tcggtttttt cataaaccgt acgccaccat tcccaggcaa gcccggtaca
    12601 ctctctaatt ttgacagaga gattacgaac attccctttt aaaggaatac tagtggtaaa
    12661 gtgagccgtt aaatcctgcc catttctgtc ccaagcctta ggagtcaaga cttccttacc
    12721 ttgatgatca taggataatt cattccaagt aatataatat tgggcaacat aggcaccact
    12781 atgatccagc agtaaatctc cgtttctgta agctgtaacc ttagtctcaa cataatctgt
    12841 actattttga aaggtcgcaa ctacattgtc acgtaaaaaa gaagttgtat aggaaatcgg
    12901 caagcctgga tgatctgctg taaagcgact gccttcttga atcaagtcct ctaccatatc
    12961 caccttgcct gttacaactc gggcacccga acttgggtcg ccccctaaaa taaccgcctt
    13021 cacttctgta ttgtccaaaa tctgcttcca ctctgtctga ggagctacct tgactccttt
    13081 tatcaaagct tcaaaagcag cctctacttc atcactctta ctcgtggttt ccaacttgag
    13141 atagacttgg cgcccataag caacactcga aatatagacc aaaggacgct ctgcagaaat
    13201 tcctctctgt tttaaatcct ctaccgttac agtatcttga aacacatctc ctggattttt
    13261 aacagcgtct acgctgactg tataataaat ctgcttaaaa ttaacaatct gaatctgctt
    13321 ttcgcctgaa tggacagagt taaaatcaat atcaagagaa ttccctgtct tttcaaagtc
    13381 agaaccaaac ttgaccttga gttgttccat gctgtgagcc gtgatttttt catactgcat
    13441 tctagctggg acattattga cctgaccata atcctgatgc cacttagcca acaaatcgtt
    13501 taccgctccg cgaacacttg aattgctggg gtcttccact tggagaaagc tatcgctact
    13561 tgccaaacca ggcaaatcaa tactataagt catcggagca cgatcgaccg caagaagagt
    13621 gggattattc tctaacaagg tctcatccac tacgagaagt gctccaggat agaggcgact
    13681 gtcgttggta gctgttacag aaatatcact tgtatttgtc gacaagctcc gcttctttct
    13741 ttcgataaca acaaactcat cgggtagctg attaccctct ttgatgaaac gattttcaat
    13801 actttctccc tgatgggtca agagtttctt tttatcgtaa ttcatagcta gtataaagtc
    13861 atttactgct ttatttgcca tcttctacct cctaataagt tcctggattg agttgcataa
    13921 actcagactt gttcagcgaa atcagccgtg gttggactaa gtaatccaaa atttcctcgt
    13981 acaattcttc tgagacattg cgtcgccgtc tggctagata agaagtcgga atgaccgtat
    14041 tatccaacat aaatacctta tctaagtcaa tcaaggttgg tcttgtaaaa ggattacgag
    14101 ctagatctgg ctcttctatc ataaagttct tgaccaaacg tctggtcaag agagctggtt
    14161 tgaaggtctg atttttaacc aactctttgt ttttagtcat gctgttgtca atacagatat
    14221 acatatgatt cttcacagcc aaatcgctac taatagtcgg aaaaggcaaa taaagagcta
    14281 caacatctcc tctcttaatc aagcaagagc accccctttt ctcctaatgt aacatagaca
    14341 ggattgacca agtcttctga ttgactcaga atttccaaag tttgagtttg gcgcgctgtc
    14401 aatttagtag catcttgtct cttcaataca aaatgcttgt cgccaataac cttgacaata
    14461 taatccttct ccaaagctga ctggtaaatc cacatcagat gttgtctgtc ctgagaactc
    14521 aagagagaag gattttcaag cctcccgata gtctgataaa aatcaaaaac aggagctaac
    14581 tcctgccaat ctgattggct agttgtcaag gctagaaaaa gggctttgcg agctgatact
    14641 tcttggttag ccttgagagt tactttcccc tccaagtttt ttagaaatcg ggaaactcca
    14701 gaaagcaaat ttttctctaa ctgcgagaaa taaaaacctt tcgttcccag acataagtct
    14761 ttcatgtcgc tttctctagc aaataagagc tcaaacattt gatagtaaaa gaaaaatatc
    14821 tggcactggg tcgcgctcat cttttcctta tcggcttctt tttttaacca gagcaagggc
    14881 gacaggtagc tggattgaga catttcctct acctcctact cttttttaac tggagcatct
    14941 gcactagctg ccacttcttt tgactggata ctttcccact ggttaatctc ctctgagata
    15001 agaccttcgc atgtcttgac aaatagggca aaagccttgg tctttcctgc atatttctcc
    15061 gtttggcatt gatagaggaa tttttctttc tccaggagtt gcgcagtttt ttggtaagaa
    15121 atccaatttt cctttgcatt atacaaattg ataatcccct cacacagcaa gccgagactg
    15181 gataaggcaa ccgaaatcaa acggtagcga tcacctggca taggaatagc acaaaagaca
    15241 gctatgagga aacctgccac gatttctgtt atttttaata ccttatagcg cctacgatgt
    15301 tgaacgcttt tctttaaaaa atgagctatc tgtacgtcta atcgctctgt caggtacatt
    15361 tcttctggcg tcatattcgt aactcctttc atttactttg ataatcatag tataacatat
    15421 ttgaatatca aagtatagta aaaactggac tataacttat tagacttcct gcgaaacaaa
    15481 atatggtata gtagttctat gaattatgaa gcaagtaaac aattaactga tacacgattt
    15541 aagctccttg ttggtgttca gcgtaccact tttaaagaga tgttagctgg attaaaaaca
    15601 gcttatcaaa aaa
//
LOCUS       DAVBCN010000048        15154 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVBCN010000048 DAVBCN010000000
VERSION     DAVBCN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15154
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.048"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(62..859)
                     /gene="proC"
                     /locus_tag="VQ497_001395"
     CDS             complement(62..859)
                     /gene="proC"
                     /locus_tag="VQ497_001395"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET2517693.1"
                     /translation="MKIGFIGLGNMGASLTKSVLQARPSDEILLANRSQVKVDAFIAN
                     FGGQTSNNEEIFEEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            complement(863..2125)
                     /locus_tag="VQ497_001396"
     CDS             complement(863..2125)
                     /locus_tag="VQ497_001396"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET2517694.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAVSFLPRLEQ
                     VLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVTGDGQIRE"
     gene            complement(2135..3265)
                     /locus_tag="VQ497_001397"
     CDS             complement(2135..3265)
                     /locus_tag="VQ497_001397"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET2517695.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMVEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRFQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(3348..5228)
                     /gene="cbpE"
                     /locus_tag="VQ497_001398"
                     /gene_synonym="pce"
     CDS             complement(3348..5228)
                     /gene="cbpE"
                     /locus_tag="VQ497_001398"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HET2517696.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSAYGNWWYQAPDSTGEYAVGWNEIEGEWYYFNQTGILLQNQWKKWNNHWFY
                     LTDSGASAKNWKKIDGIWYYFNKENQMEIGWVQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTGGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SE"
     gene            complement(5231..6118)
                     /locus_tag="VQ497_001399"
     CDS             complement(5231..6118)
                     /locus_tag="VQ497_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2517697.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(6108..6569)
                     /locus_tag="VQ497_001400"
     CDS             complement(6108..6569)
                     /locus_tag="VQ497_001400"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET2517698.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMFLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(6566..7474)
                     /locus_tag="VQ497_001401"
     CDS             complement(6566..7474)
                     /locus_tag="VQ497_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET2517699.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            7467..7664
                     /locus_tag="VQ497_001402"
     CDS             7467..7664
                     /locus_tag="VQ497_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2517700.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            complement(7716..8387)
                     /locus_tag="VQ497_001403"
     CDS             complement(7716..8387)
                     /locus_tag="VQ497_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2517701.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(8441..8662)
                     /locus_tag="VQ497_001404"
     CDS             complement(8441..8662)
                     /locus_tag="VQ497_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HET2517702.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAARF"
     gene            8934..9743
                     /locus_tag="VQ497_001405"
     CDS             8934..9743
                     /locus_tag="VQ497_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2517703.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(9924..10799)
                     /gene="aguB"
                     /locus_tag="VQ497_001406"
     CDS             complement(9924..10799)
                     /gene="aguB"
                     /locus_tag="VQ497_001406"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HET2517704.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKLLAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYNLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(10809..11894)
                     /gene="aguA"
                     /locus_tag="VQ497_001407"
     CDS             complement(10809..11894)
                     /gene="aguA"
                     /locus_tag="VQ497_001407"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HET2517705.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVV
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(11891..13018)
                     /gene="nspC"
                     /locus_tag="VQ497_001408"
     CDS             complement(11891..13018)
                     /gene="nspC"
                     /locus_tag="VQ497_001408"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HET2517706.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(13018..14277)
                     /locus_tag="VQ497_001409"
     CDS             complement(13018..14277)
                     /locus_tag="VQ497_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HET2517707.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVITLIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMLD"
     gene            complement(14274..15134)
                     /gene="speE"
                     /locus_tag="VQ497_001410"
     CDS             complement(14274..15134)
                     /gene="speE"
                     /locus_tag="VQ497_001410"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HET2517708.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
BASE COUNT         4436 a         3505 c         2770 g         4443 t
ORIGIN      
        1 agtttgaatt tgattttcga agagtatcag ccaccataaa agaggcagat aaaactacct
       61 cttatttacc tagttcttgt gttcgtttat aggcttgatg aactgcctcc atgactgtgc
      121 ctcgaaaagc atgcgcttct aggcttgcta cgccagcaat agtcgaaccg cctggactac
      181 aaacttgatc tttcaaaact ccaggatgtt gctggctttc taggaccaat tgcccagctc
      241 ctaccacagt ttgggctgcc attttcaagg ctgtttctcg tggtaatccc gtctgaacac
      301 ctgcatctgc caaggcctca ataaagagat agacaaaggc tggtccacaa cctgcaagac
      361 ctgtcgctgc attgattaag ctttctccta attcaaccaa gagaccagcc ttggctaaaa
      421 gctgacaaaa gatctcactg tcctcagccc tgcaattagg agacaaggca taactaatca
      481 ctccttgccc gatagaagca ggggtattag gcatcatacg aataattcgg tgttggcttg
      541 ggataagact tgctagtttt tctaaggtca atccagctgc catcgaaatc aaaagaagac
      601 tttctctttt ttcaaggatg gtctggtatt gagaaagcag ttcagaaaac tgagcaggct
      661 taacacctag aaaaatcaca tctgcttctt cgaatatttc ttcattgtta gaagtctgac
      721 caccgaagtt agcgatgaaa gcatccacct tgacttgact acgattggca aggagaatct
      781 catctgacgg tctagcctgc aagacagact tggttaaact agcacccata ttccccaaac
      841 cgataaatcc aatcttcatc tcttactccc ttatctgtcc atcaccagtg accacatact
      901 tgtagctagt caattccttc aagcccatag gaccacgcgc gtgcaatttc tgagtagaaa
      961 tccccatttc acaaccaaga ccaaattgtc ctccatctgt gaaacgagtt gaggcattaa
     1021 catacaccgc tgcagagtcc acttgatctg taaagtatgc tgcagcttca gcattttccg
     1081 tcacaatggc atccgaatga tgggtactgt gggattcaat atgcgcaacc gcttcttcta
     1141 aactgctcac aaccttaaca gctagaatat agtctaaaaa ctcggtatca aagtcttgtg
     1201 cttgagcagc ttgacctgaa acaaactggc ttgctttgct atctaggcgg aattgaattg
     1261 gttccaaccc agcttctttt cgatctgcaa ccaacacttg ctccaagcga ggaaggaagc
     1321 ttactgcttt gttttcatga accagcagaa cctccatggc attacaaaca gaaggacgac
     1381 tggttttagc attgttgatg atagacagcg ccttgtcttc gtctgcatcc ttatccacat
     1441 agacatggac aatcccagtt cctgtctcga taacaggtac aatcgcattc tcaaccactg
     1501 cattgatcaa gccagctcct ccacgaggaa tgagaaggtc tagatagccc ttggccttca
     1561 tcatagcata actactttca cggctagtat cctccaccag ttgaatcaca tttggatgaa
     1621 tagtagtcgt ctccaagccc ttcttcaagg ctgtgacaat ggcatgggtt gtttgatagg
     1681 catccttacc actacgaaga acaaccgcat ttccactctt aagagtcaaa gcagccgcat
     1741 cagacgtcac atttggacgg ctttcataga taataccgat gacacccata gctacacgtt
     1801 tttttgtgat aaccaaacca ttttcaagct gacttgtttc taaaacttca ccgattggat
     1861 ctggtaaggc aaccacttca cgaattcctc ttgccatcgc ttctatacga tctgcatcca
     1921 aataaagacg atccaacatc acatctgaga ttttcccctt agccgctgcc atatcgaggg
     1981 cattagccgc taaaatttcc tcagtagcag ccactaagtg atcagccatg gctagcaagg
     2041 cttggttttt cacttcttca ctagctgtgt taatcgattt tttaacagcc tgtacctgtt
     2101 caaattgttc ttgtctactc accatagttt acctctaaaa ttctgtaaaa agtagttgga
     2161 tttcaggagt aatggaaatc cagtcgtcac ggtaaatcaa gacacccttg gctttttgaa
     2221 aacgcaacat atcctccaaa gcagatgctc caaattgcac gcgtcctttt ccaagtgatt
     2281 ttccactttc cttatcaaat actgtcacga tatcaccgta agaaaagact ccttctgctt
     2341 caacgatacc agataagaga agactctttc catgttgaga gagagcttcc gcagcccctt
     2401 tatcaaccca aatagaacct tgactctgag catagaaggc aagccattgt ttctgggtac
     2461 gaagcccctt ctcttgagca acaaagtaag aaccatcctc ggtctcctct gccgcctcaa
     2521 ccatggaatc tgatttcaag gatgagcaga tataaacagg aactcctgat tccgtcgcga
     2581 tagttgcagc cttgattttg gttaacatac ccccagttcc gtttgacgaa ccagctccac
     2641 cagccatatc aataatctca cgattgatgg tctcgattct ctccaagcgt ttggctcttg
     2701 gatctgaatt aggatttcca gtatagagac cgtccacatc tgtcaagaaa actaaaaggt
     2761 ctgcttggac catcgccgct acttgagcac ttagagtgtc attgtcccca accttgagct
     2821 catcaataac gacactatca ttctcattga tgataggaat tgccccacgg ttgagcaaaa
     2881 ccgacaaagc ctgatgggca tttttataac gacgcttatc cacaaagtca tcttgggtca
     2941 gcaagatttg tgcagaaacg atttgacgca agagaagatt ggtcgtatat tcttccaaca
     3001 aaagtccctg ccctaccgct gctgaagcct gtttatcagc aattttagtc ggacgctttt
     3061 taaatcctaa ggctccaaaa ccagccgcaa tggcacctga agacaccaaa atcaactcat
     3121 gaccagcctc gtgcagcata gccaactgct gggtaatatc ctttacctta ctacgtgata
     3181 aacttccatc ctcatttgtc agagaagaag tgcccacctt aaagacaatc cgtttgtatt
     3241 tcatagtctc actccgatta ttcatattta tccattataa catgaataac aggaaataga
     3301 aaagagttga taggaaaaag aaggccctaa aaccttcttt tctactacta ttctgattcc
     3361 gatttgttta cactcgtttc agagctttgt gaagttgagg atgtcaaatc ttgactagtt
     3421 gaagaatttg taacactttc atgttgactc gtttctttat tttcaagatc ctttttcact
     3481 acttctttgg attcttttat ttctgaatga cttgtagttt taattgtagc agatttctcg
     3541 atcggaacat ccactaacca ggcaccactt gaatccacag tatagccttc tggtgtctta
     3601 ccattcacaa gtaattgacc attttctttc aagaagtacc atttatcctt gtctttaatc
     3661 caacctatag ctcgtgaacc acctgtcttg tagaaatacc aatcatttgc ctttttaagc
     3721 cagccttgct tcatagctcc tgattcttct agatagtaat gatgtccagc aacttctatc
     3781 tcacctgtct tcatgatacc agtagaatcc atatagtacc aggcttcttt atcttttacc
     3841 cagcccgttt tcatttcccc tgatggagca aagtaatacc attgcccctt atattgaagc
     3901 caacctgtct tcatagagcc atcaacatcc aaataatacc actgctcttt atcttgaacc
     3961 caaccaattt ccatctgatt ttctttgtta aaataatacc agattccatc aattttcttc
     4021 caatttttag cagaagcacc agagtctgtc aaatagaacc aatgattgtt ccattttttc
     4081 cattgattct gtaacaagat acccgtttgg ttaaagtaat accattcacc ttcgatttca
     4141 ttccaaccga cagcatactc tcctgtagaa tcaggcgctt gataccacca gttcccatat
     4201 gcactcttat gccaaccagc ttgaaaactt ggaatcggct tgtaggatgt tgaaatattg
     4261 acaaaaccgt cttttcgaat atcaaaaact gttgcatcat agtctttgct ggctgcgttg
     4321 attctctcaa ttcctcgttc tttgagccaa ttaacatact cactatcaac accatttttc
     4381 caaggtagac tatccgaagt ttgaacaatc aaactcggac tcaaattttt aatgaaatcc
     4441 ttggtatttg atttgttggt atcatggtga tgattaaact tcatcaaatc aacttttcca
     4501 atgagaggac catacttgtc ttctgctcca tgaacattat ctaaatcgcc cccaaggtaa
     4561 attttcttgc cattgacttt caccacgcta atcaaggaat tggaattgtc atcccaaatt
     4621 ttctttaatt cacccgatga atcagtttca ttttcataat tatagagctg aatatccatg
     4681 tccccaaact gaaaatgagc atccccttgt gtgatatttt gaataactga aacacctttt
     4741 tctgcagcag tctgtaaaac cttatcatag ccatacagat tatcccatag acgttcagaa
     4801 ttagtaatac gactatcact atatttctta agatagactc ggtcaactgg ataggtagac
     4861 agtaattcat caacatttcc aatatgatca ctgtgggtat gggtcaccaa aataaaatca
     4921 agtttttgga cacccaattc cttcaaacga cgaaagacac ggtctgttag aacatgctta
     4981 taagacgttt caattccttc tctccatgga taacgagaat cacttccatc tgggaaatca
     5041 taatcttctc ctgtatccac catggcaaaa tgtccattgc tttcaagaat aatcgcatca
     5101 ctgccacctt cttgaacatt gataaagtgg attttatttc ctgaactttc ttgagcctga
     5161 acattcccat accatgacaa acctaaaaaa gcgcctacaa gtgctaaact agttaatttc
     5221 tttttcattc ttactttctc aatctctcca aggtttcctt aaaaatctct gggatatctg
     5281 ctttaaattc caaggtctta cctgttctcg gatgagtaaa acctaaagtc ttggcatgaa
     5341 gaaattgtcc atgtcctttc aaagtcttgc gaggaccata gacctcatca ccagcgactg
     5401 gatggccgat ataagccatg tggacacgga tttgatgagt gcgccctgtc tccagttgca
     5461 actctactaa gctataatcg ccaaagcgtt ccaagacgtg aaaacgcgtc actgcaggct
     5521 tccctttagc agttacagcc tgtttcttac ggtctttttc actccggcca atcggcgctt
     5581 caattacacc acgatcatta ggcagatttc catgaacaat cgcccaatat ttgcggagag
     5641 actttttatc tttgagttct tgggcaagta ctagatgcgc atcatcgttt ttagcaatca
     5701 tgagaagacc tgacgtatcc ttatcaatac ggtgaacaat ccctggacgc agaaccccat
     5761 tgatacccga caagtcctta atatgataca tgagggcatt tactagggtt ccactggtat
     5821 gaccagcact cgggtgcaca accattccct gaggtttgtt aacgacagcc acatcctcat
     5881 cttggtagac tatttctagc ggaagatcct cagccacata ctctaatacc tctggttctg
     5941 gcacatggta agtgacgaca tcaccctctt ggactgtgta tttagctttc ttgacttgac
     6001 cattgaccaa gacctggcct gatttaattt gttcattcgc gagactacgt gataattctg
     6061 acaaatctga caaagcctta tccaaacgca gaccaccagt ttcaatttta atttccattt
     6121 atttcctctt ttagcattgc aatcaataaa ataatcactc caaccgtcag atagctatct
     6181 gccacattga aaattgcaaa gttgataaag tcaaggtgga acatatccac aacaaagccc
     6241 tgactgaccc tgtcaataaa gtttccaaga ccacccgcga ttattagagt caaacccaag
     6301 aacatccaga atgagtcctc catgtgttta tgtaaatacc aaatggcacc tatcacgaca
     6361 accagagtaa tgacagcgaa taacagctgc tgatcttgta agatagaaaa ggctgcacct
     6421 cgattttgca ggtaggtcaa gctaacgaaa ttgggaatcc aggagcgcac ttcacccagt
     6481 ggaatctgct ggacgatata ggatttgacc aactgatcca gcccaattaa aagcagtaca
     6541 atgactgcca ctattgctct ttttttcatg atttcctctt ttgatcaaaa tattcttgca
     6601 tgacttctac gaagagagtc ccagcttgac taagctccac ttcttcacgt ttaacataga
     6661 ccatgcggtt atctaggtta tccttgagac gaataactgt aatgccatta acactgtcac
     6721 tatctaaaaa tccagaacct gtcgcatagg cgtccgtccg ctccaaaata ccattcaagg
     6781 tggcacggtc tgtcacatta aacatctgtg agctagcgct ggtatcgaca aagttctctg
     6841 aataataaag gtactcgtct ttctcttgag tgaaacgaac cgttggtaaa tccgctaaat
     6901 cctccatgac taattcctct ttctgggcta aaggatgacc ctcacagaga taaatatggg
     6961 tatggaaagg aatcaattcg atgacctcca gacctaactt ttcaacccgt tgcataatcc
     7021 cctttttatt ttgattgttg aggtagataa tcccaatctc actatgccct tgcgccactt
     7081 catctaatat ttgaacagta gttgattcaa aaatacggaa gttcttatag tcaggatagc
     7141 gctctgaaaa ggccgtaata gttggtggta agaagtcata gtgctggcta gcaacggaaa
     7201 attcatcttt ttcttcttca ggattggcat actgattttg aaaaatatca aatcctttaa
     7261 ccaattcttg cgctttttca taaaattcca tcccacgacg ggtcaagaaa gtccctgagc
     7321 tggtccgacg gaaaatctta aagcccaact ctttttccaa atcacgaaca gaaatagaca
     7381 gactcggctg actaacatac atcttttcag cagcttcacg aaaagtacca ctattggcaa
     7441 tagccacaac atagcgtaat tgttgaatgt tcatcttcta cccccaactt ctttatcttt
     7501 tcattatacc atattttaga agttttccaa aaaggaaaaa agaacatcct attcttctta
     7561 actatcttca ctatctgcct ttctcacgcc aatcttattt tcaaaatcaa ttcaaaaaaa
     7621 taagtggcta cacaccactt cagtatcgga aagaaaaacg ttgactattt gtgaaaaaag
     7681 aaaatgccgg aaaattccga cattttttta gtaagctaac ttcctgaaaa taggagtaac
     7741 caaacatccc agaaggcagt gatattgatg agaatatgaa ctagtattgg atagtagaga
     7801 tttttagtca tgcgatacaa tagagctaaa atcagacctc cactagcata aatgaaaaaa
     7861 gcaagaggag ttaaagcaaa attgatatga atataaccga aaataatagc agaaagcaaa
     7921 acatctccgt accaaggtga gtttttgaaa aaggttgtca taagcacacc tcgataaatc
     7981 aattcctcag caataggggc gatgaagcaa acgatgagca agaaataagg gaactcctgt
     8041 ctccccatca tttctaccgt ttcattcaaa gaaatctgat ttgaagacag ggatatgaaa
     8101 tacgaaaaga ggaagtcaga catatatgaa atgatgtagc ccagtaaaag gtagatgaag
     8161 tacctcagct gccacttgaa atgaaaaatt ttctttcctg caaaaaccag tagatagatg
     8221 accgctaaga aaagaactcc gctctccatc aaaagcagaa tctgaaaaaa ttcacgactt
     8281 gctggtagat agggctttgc gaggtgattg aaaactctcc aaaaccaagt tgatttgtaa
     8341 aaaattaagg acaatgctag taaaatttga atagcccgtt ttttcatatt aaattctctg
     8401 ctttctccac ttatttcttc atattactaa taaattttac ttaaaatctc gcagcacttc
     8461 ctttgcaaag atgattgcct cctccaatat atcctaaatc ataggtgcct ctgggacaaa
     8521 atccatgaac cggcagagtt aatactggtt ctatttccca atcgtaccta ccattaggat
     8581 ttttaccata tgaatctgct ccaccttgta tcagctctaa ttcttcattt gttagattta
     8641 tattttttgt cattaaaatc atgatatatt tcctccatta ttcttcagat tagttgagta
     8701 atctgatact catacctact tacaaaaaaa ctattatatt aagttggttt tttaatttgt
     8761 ctagttgcaa cattgacaaa catagtatag catatctttg cgaaatatcc tcttcaaatc
     8821 atgaattgtc atcaaaacat cttaaactat aaaatcaatt agtctcaagc tttctatcaa
     8881 tttcttctcc aaaatatgct ataataatag caaaagataa agaaggaaga cctatgatta
     8941 aactactagc cttggatatg gacggcaccc tcctcaatga agccaaggaa atcccacaag
     9001 ctcacattac tgctattcac aaagccattg aaaaaggtgt caaactggtt ctctgtacgg
     9061 gtcgccccct tttcggtgtc ctcccctact acaaaaaact gggactcgac ctccagaatg
     9121 agtatgtgat tgttaacaac ggttgttcaa ctcaccagac tagcgactgg ggcttggttg
     9181 actggcaaga acttagtcca gctgacatcg aatacctcta tgaccttgct gaaaagagtg
     9241 atgttcagtt gacacttttt gacgagtcac attattttgt tctcggtgga aagcccaatc
     9301 aagttattga aaatgatgct aaactagtat tttcagacct gactgaaatt tctcttgagg
     9361 aagcgactag tggaaagttc cggatgttcc aaggtatgtt tttaggaaca aaagaacaaa
     9421 cagacgattt tgagcagcgt tttgctgaag agctttgcca acgattcagt ggagttcgtt
     9481 cgcagcctgt catttatgaa gcaatgccac ttggaacgac aaaggctact gctctttcac
     9541 gactagctga gattttgaag attgattcct cagagattat ggccatgggc gatgctaata
     9601 acgatatcga aatgctccag tttgcagggc ttgggattgc aatgggaaat gccagcgatt
     9661 atgtcaaatc tcttgcggat gccgttacct caagcaacga agaagacggc gttgcgcgtg
     9721 ctattgagaa atatattcta taaaaaagaa aagcaaatag acagaagtta ctgctatttg
     9781 cttttttgct aatattttaa aatacggact aatcaataga gaagaatagc gaatcataaa
     9841 aatcgattta ctagatgcca tagtatctta tagttgctaa taagaagtta gtctagcaga
     9901 atgaatctct catttctccc acactaatct gtaatttgtc tatacatttc tggtcttcta
     9961 tctcgaaaca agccccagtt taggcgttca cttgctccct tgtctaggtt ataagtagct
    10021 aacagaacag cttcttcttg tctttcagct cgttctagaa tagctcctgt ttcatccgtc
    10081 ataaaggagg aaccgtagaa gtcaagactg gaactctgtc cgccattttc ctcactagga
    10141 gtaacctcct ctaaaccata acgattggct gcgatgactg gaacaatatt cgctgctgcg
    10201 tgcccttgca tagtacgttg ccagtgacca caactatctg tatccaaaat tggctctgaa
    10261 ccgatagctg taggataaaa gagcaattca gcaccattca atgcaagaca gcgcgctgtt
    10321 tcagggaacc attgatccca acagataccg ataccaatct tagcatagcg agtattccag
    10381 accttgaaac cagtgttacc aggcgtgaaa tagaattttt cttgataata atggtcatct
    10441 ggtatatggg tctttcgata aacgcccagc acttccccat ctgcatcaat gacggcaata
    10501 gagttataca agacattacc atctttttca tagaaactga ttggtaaaac aacttgtagt
    10561 tccttagcaa gcaacttaaa atgctgaatg gcagtatttt ccgctacaga ttgggcatac
    10621 tggtagtagt catactgacg ttcctgacag aaatagggat gttcaaacaa ctcgggcaag
    10681 agaataattt gggctccttg ctcagcagcc tgacgtacta aacgctctgc ggtttggata
    10741 tttgttgcca catccttagc gcattgcatc tgaatggttg caactcttac atttctcatc
    10801 tttttctcct attctggaat ttgttgggtg atacagtgga tattgccacc acctaagaga
    10861 atatctctgg ctggtattcc gacaacttta cggtctggga aacacttgct gaggatatct
    10921 aaggccactt ggtcgtttac atcctcaaac tgtggaacca agacagcctt gttggcgata
    10981 taaaagttta cgtaggaagc tgctagtcgt tcacctgcgt atcgctcttc ttctccttct
    11041 tcatagatgt agcctggcaa atcttcttct gtcacaactt gtcgaactgc agggataggc
    11101 aatttatgaa tggtgaagtg acaacctttt gcatctgttt cctgttctaa gagttcgaga
    11161 tctgcttttg acatggcata ctggggatca ttttcgtcat ctgtccaagc caaaacaacc
    11221 tcagcaggac caacaaaggc aacaacatta tcgacgtgtt cattggtttc atcctgataa
    11281 ataccataag gaagccaaat aactttttca gcaccaagac tttctaataa tgtgttttca
    11341 atctcctctt tagtcaagtt aggattgcga ccaggactaa gcaagcaact ttcagttacg
    11401 agaatagttc cttgaccatc gctatggatt gcgcctcctt ccagtacaaa aggtttagca
    11461 tcatagacag gcctttccaa ggcctcagca aaacgactgg ctacttggtc atcctcttca
    11521 taatcttgat aaagaccatc ataggtgcct ccccaagcat taaaggccca atccacggct
    11581 aatttcttac ctttatcatt gacgagaatg gttgggccag tatcacgcgc ccaggcatca
    11641 ttggtgggaa tgtctaaata aacaaccttg tctccaagat aggattgggc ttcagataga
    11701 taggcctgct ccaccaaaag atagactctt tccccttctg ctatggtctc gatgatctga
    11761 gtaaatgctc ttttagcagc ctttccttga aaaggccatg atcctggtcg agtcggccat
    11821 atcatgaggg taccatgatg gggttcgtac tctgctggca tgtgatagcc taattttttt
    11881 ggactgtcca tcatgataat ctccctttaa agtcttgata gccaaaagct ttgagtaagc
    11941 tacagtctcc ctgttcgtcc atgagataga gacttggcaa tccaatacca ttaaaggtat
    12001 tatttttgac aaaagaataa atggccatgt cttgaaaata aagtctgtct ccgatttgga
    12061 ctggattttc aaaactataa tcaccaatca catcgccagt cagacaggta ttagaagaaa
    12121 gtctgtaggt atgggctttt tcctgtgact caaagccatt tctcaaaggt ggacgatagg
    12181 gcatctcaag tacatcaggc atatggcagg tcgcagaggc gtctaaaacc aagatttcca
    12241 taccgttttc tacaatatct aatacctcag ttgctaaata acccgcatta agcgcaatgg
    12301 cttcaccagg ctcgatatag atttcaagat tgtaagtttt tcggatacgc ttgatttctg
    12361 aaatcagcaa atccacatcg taaccttctc ttgtaatatg atgaccacca cccatattga
    12421 gccattttac ctcatgtaag tagggaccaa actgttcttc tactgctttc aaagttgttt
    12481 gtaaatcatc tgctccctgc tcgcaaaggg tatgaaaatg aagtccgtca accaaatcta
    12541 gcaaatcgct cggaatcttg tctatagtaa ctccaaagcg agaaccaggt gcacaagggt
    12601 catagagcgc gtgatctcct tgagttgaac actgagggtt gaggcgcaaa ccaacactga
    12661 caccagcctc tcgacaacga gccccatgct tacgcaactg tctctctgag ttaaagacga
    12721 tatggtctgt tatcccaagc aattcctcca tgtcctcatc cttgaaagct ggtgcaaaaa
    12781 catggacttc accaggaaat tcttcccttg ccaatttggc ctcatagagt ccactagctg
    12841 tcgtacctga tagatactgg ctaatcaagg gataagtttt gtagagggaa tatgccttct
    12901 gggcaagcaa gaccttgcaa ccggcctctt cttgtacata ttgtagaatg cggcaattag
    12961 cttctaactt ggccaagtca ataacatagg ctggtgttgg tacttgttct aacttcatta
    13021 gtccaacatt tgtggatttt caaccacaac ccatggcaaa ccatactcat tcaaagcttc
    13081 catgaatgga tctggatcta actcctcaag gttatacact ccagcttgtt tccaagttcc
    13141 gttcatgact aattttgtcc caatcatggc tggaacgcct gtcgtgtatg aaattgcttg
    13201 tgacccgact tctgcatagc actcttgatg gtcgcaaaca ttgtagatgt agatagtctt
    13261 ttcaacgcca tctttgacac ctgtaaagat acatccaata ttggttttac ctacagtacg
    13321 tgggccaagg ctagcaggat ctggaagcaa ggctttcaaa aattgaattg gaacaatttc
    13381 ttggccgtta aagttaatgg tatccgtacg aaggagtcca acattttcaa gacatttcat
    13441 gtgcgtcaag taagattgac caaaagtcat aaagaaacga atgcgtttga cacctggaat
    13501 gttcttggcc aatgattcga tttcttcatg gtgaaggaga tacatatctt tttgtccaac
    13561 ttgagggaaa tcatactcac gcttgataga catagcttcg acttcgaccc atttcccatc
    13621 ttcccagtaa gaacctggcg cagaaacctc acggagatta atttctgggt tgaagttggt
    13681 tgcaaatgga taaccgtggt caccgccatt acagtctaaa atgtcgatat aatggatttc
    13741 atcaaaatag tgtttgaggg cataagctga aaagacactg gttacacctg ggtcaaaacc
    13801 agaaccaaga agagcagtca agcctgcttc tttgaatttc tcttgataag cccactgcca
    13861 tgagtagtca aagtaggctg taaaaccaag ttccttacaa cgtttttcgt agatagcacg
    13921 ccactcaggg tcttctgtgt cttctgcttc gtagttggct gtatcgatat agtgaacacc
    13981 tgttgccaaa caagcatcca taatggttaa atcttgataa ggcagagcta cattcaaaac
    14041 agcttctggt ttgtagcttt caatcaaggt aatgacctct tctaccttgt cagcatcaag
    14101 tgctgcagtt tcaattttag tacttgtttt gccttctagc ttcgctttca agtcatcgca
    14161 ttttgacttg gtacggctag caatcataat ctctgtaaac gtttcgctat cttgacaaat
    14221 ctttgaaata gcaacttggg caacgccccc acaaccaata actagtaaac gactcatttt
    14281 tttccttcct cttcttctaa aatgtcctca acatacttgg gcaacataaa ggctcccacg
    14341 tgtaagtttg cagtgtagta ttctgtgaaa agctggcgtt ttttccagcc ttccttgtca
    14401 aaatctttga cagggtggta ttttttcgat gcaaatccaa acaaccaata gccagctggg
    14461 ctagttggaa tatgggcctg ataaacccga ctgattggaa aggcttgatt gaccttgcgg
    14521 tgcatgcttc ggcaggccga ctcatcctcg tcaaagaagg gactcccatg ctggtaaatc
    14581 atgatgccgt cttccttcag agctcgataa ctattgccgt agaattcctt ggtaaagagt
    14641 ccttccgtat ggccaaatgg atctgtcgca tcgttgatga taatatcgta atcatcttcg
    14701 cagtttcgca aaaagcgtag cccattttgg tagtaaatgg taacacgagg atcatctagc
    14761 cctgcagcaa agtctgggaa atactcacga cagacctcga ccaacatctc atccggttcc
    14821 acgatatcga tttgttccag ttcaggatag agtgttaata cttgggcaac accgccgtca
    14881 ccacccccaa taaccaatac tttctttgga tttgggtgga cagccatggg aacgtgaacg
    14941 gtcatttcat tgtagacgaa atcatccgca tctgagaaca agacatggcc atttaaaatc
    15001 agtattttcc caaaagctgg cgtatccaag acttcgatat cctgccattc actttttcca
    15061 gcgtaaagtt gcttggctgt tctcagagac aatttgacat ctggagtatg aacttcagaa
    15121 aaccataaat ccatctattt ctccttatat tata
//
LOCUS       DAVBCN010000049        14953 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVBCN010000049 DAVBCN010000000
VERSION     DAVBCN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14953
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.049"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(114..746)
                     /locus_tag="VQ497_001411"
     CDS             complement(114..746)
                     /locus_tag="VQ497_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2517709.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(768..1640)
                     /gene="sdaAA"
                     /locus_tag="VQ497_001412"
     CDS             complement(768..1640)
                     /gene="sdaAA"
                     /locus_tag="VQ497_001412"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HET2517710.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNEKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            complement(1649..2320)
                     /gene="sdaAB"
                     /locus_tag="VQ497_001413"
     CDS             complement(1649..2320)
                     /gene="sdaAB"
                     /locus_tag="VQ497_001413"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HET2517711.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            2562..3134
                     /locus_tag="VQ497_001414"
     CDS             2562..3134
                     /locus_tag="VQ497_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET2517712.1"
                     /translation="MKSITKKIKATLAGVAALFAVFSPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYSAE
                     NQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            complement(3186..4049)
                     /locus_tag="VQ497_001415"
     CDS             complement(3186..4049)
                     /locus_tag="VQ497_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2517713.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSKHAEQVVKKLWKKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKK
                     EVEIYYQAKER"
     gene            4515..4853
                     /locus_tag="VQ497_001416"
     CDS             4515..4853
                     /locus_tag="VQ497_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HET2517714.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKINGTRITK"
     gene            4831..5190
                     /locus_tag="VQ497_001417"
     CDS             4831..5190
                     /locus_tag="VQ497_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517715.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WILPILLMAGMGDVIIN"
     gene            5209..5421
                     /locus_tag="VQ497_001418"
     CDS             5209..5421
                     /locus_tag="VQ497_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517716.1"
                     /translation="MMVVLISVIVAFLYHKITEYVNNRKIDIVIFLSLLISHFIWNYY
                     FLPIEILIAISGANLLLSFLKKLQIK"
     gene            5836..6300
                     /locus_tag="VQ497_001419"
     CDS             5836..6300
                     /locus_tag="VQ497_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517717.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGGVGCNWWNKRDNIAAAAVSGG
                     MSVYQTYAIKKLIANRGMMSRVVRSAITRYIGGVSMATITNALNIAATVAEVFSLGGA
                     IAYGLDIVDGKFDGYNKINLINSIIIALGMILALILVLSRVYRKYFYRDDDD"
     gene            6457..7515
                     /locus_tag="VQ497_001420"
     CDS             6457..7515
                     /locus_tag="VQ497_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517718.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIESKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREIALLLATIIVYFFTHRILPLSVFTFIFSYILLFAQ
                     SYLGGNTVWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNLTPIILLAI
                     FENLLDSYLLQNQSEVDLDIFYKVLPLLYKEKYTMGFNYFVSLNYLLYKVGFLGIIYD
                     NEALGDLSKQYLNRNISELQDGSFDGGIQDAVASKQIVVINEFIACLNSRCMPSQYDR
                     FFYKDRPYIFSRKSPIKG"
     gene            7830..8804
                     /locus_tag="VQ497_001421"
     CDS             7830..8804
                     /locus_tag="VQ497_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517719.1"
                     /translation="MKNKRYFFDTILMILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLIPFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLKKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYMGIKHVSTK"
     gene            9184..9558
                     /locus_tag="VQ497_001422"
     CDS             9184..9558
                     /locus_tag="VQ497_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HET2517720.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGFSIPRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
     gene            9766..10668
                     /locus_tag="VQ497_001423"
     CDS             9766..10668
                     /locus_tag="VQ497_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517721.1"
                     /translation="MIRKLIVFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEGLIKIFPFIFIRRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWQKEDSFESNLHKILMDKIQKSELSYSDLMSLLLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEISISNNNNWQEKVDKIRIQLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            10724..10963
                     /locus_tag="VQ497_001424"
     CDS             10724..10963
                     /locus_tag="VQ497_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET2517722.1"
                     /translation="MELVLPNNYVVIDEEVMMYLDGGAYLSKRACQGICAAAGVLATA
                     AGKIAYCIGYGVLNRGCDINENPYPWDGFISATVR"
     gene            10960..11688
                     /locus_tag="VQ497_001425"
     CDS             10960..11688
                     /locus_tag="VQ497_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517723.1"
                     /translation="MSNVDKIRKIHIIVCWVYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLVINSILLMSFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IRKDFDIKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGKVDNHVLTLI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSVPFIKEKEYELFKNSK"
     gene            12823..13062
                     /locus_tag="VQ497_001426"
     CDS             12823..13062
                     /locus_tag="VQ497_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517724.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLYKITRDYASQTKPAIQDGVFILEDFRNILCTMM"
     gene            13612..>14953
                     /locus_tag="VQ497_001427"
     CDS             13612..>14953
                     /locus_tag="VQ497_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein"
                     /protein_id="HET2517725.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEEAPVASQSKAEK
                     DYDAAVKKYEAAKKEYEDGKAAQKKYEDDQKKTEEKAEEERKASEEEQAANLKYQQEL
                     VKYIRENDPTKKAEAKKAMDEAEKEYKKKQTEFAEVRAKVIPSAEELKKTRQKAEEAK
                     AKEAELTKKVEEAEKKVTEAKQKVDAEHAEEVAPQAKIAELEHEVQKLEKALKEIDES
                     DSEDYVKEGLRAPLQFELDVKQAKLSKLEELSDKIDELDAEIAKLEKDVEDFKNSDGE
                     QAGQYLAAAEEDLVAKKAELEKTEADLKKAVNEPEKPAEETPAPAPKPEQPAEQPKPA
                     PAPQPEKPAEEPENPAPAPQPEKSADQQAEEDYARRSEEEYNRLTQQQPPKAEKPAPA
                     PQPGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNANGAMATGWLQYNDSWYYL"
BASE COUNT         4935 a         2245 c         2910 g         4863 t
ORIGIN      
        1 caaccttaaa acagtgtttt gagcagcccg cagctagttt cctagtttgc tctttgattt
       61 tcattgagta ttacttcatt ttcttctgaa atagagttgt cgcccagtct atgctattga
      121 aaatacgcca aaacttctaa gggtttgtga gcgatataat caggttgata gtttagtaga
      181 tctgcttgct ctccaaatcc ccaagtgacg gccaatttct gaatacctgt ttctcgagct
      241 cccagcatat caaacttggt atctccgatg atgatggctt gttctggtgc tagttgatgt
      301 gtctgtaagg cttggtgaat gacatctgcc ttatggggtg cttcagggct agaaccataa
      361 ataccatcaa agaaatgatg gatttccaag tttttggcca tgtcttgagc agtagatgta
      421 ttctttgtcg tggtgatgta gagtggataa ctgctcgata actcctcaag caagtctata
      481 atctgaggaa agagttgagc ttcatagatg ccttttgcct tatagtaaga acgatatatc
      541 tgcatggctt cagaaatttg gtctttggac aggcaggtcg caaaactact ttcgagaggt
      601 ggtcccataa aaccacgaat agttttggca tcagggctag gcacccccag ctctttaaag
      661 gtataggtaa aggcattgtg aatcccgata gaactatcaa cgagggttcc atccaaatcg
      721 aaaaaaatcg ctgtgataga ggtcatggtt tctcctattt gataagctta ttctccgaaa
      781 atctcttttt ggaggcgacg accagtaggg gtggtagcga gtccaccttc agctgtttca
      841 cgaaaggcag ttggcatgct tgctcctact tggtacatgg catcgatcac ttcatccaca
      901 gggattttag attcgatacc tgccaaggcc atgtctgctg cgatgaaagc aaagctagct
      961 cccatggcat tacgtttgac acagggaact tcgaccaaac ctgcaacagg gtcacagatg
     1021 aggcctagca tatttttaat gacaaaggca atagcttgac tggcctgata aggtgttcca
     1081 cctgcagcca gagtcaaggc ggcagcactc atagcagagg ctgaaccaac ttcagcttga
     1141 cacccaccct cagcacctga gatggaggca ttgtttgcga tgactagtcc aaaggcacca
     1201 gcagcaaaga ggaaatccaa ttgttgctcg tggctgaggt ctaatttttc aatagcagca
     1261 gtgagaacgg aagggagaca accggcactt cctgcggtag gagtagcgca gaccaagccc
     1321 attttagcat tgtgttcatt gactgcgata gcatttcggg cagcagagag aatcgtgtaa
     1381 tctgataaag ttttttcgtt tttgatgtag tgatccaatt tggcagcatc tccacctgtc
     1441 aggccactac gagatttatt ttcattgagg ccaagttgga cagaggcttt cataacttcc
     1501 agattgcgtt ccatgagaag gaagacttct tcacgttcgc gaccggtcaa ttcaaactct
     1561 gttgtaatca tgagttctgc gacatttcct tgaaagtcca gatctgcttg ctcgaccaat
     1621 tctttgatag aataaaacat gcttcctcct atttaaagaa attgacattg tggagatgag
     1681 ggatttttcg aatttcttcg atagcctcat cacagttgcg actgtcaact tcgataatca
     1741 taatggcttt ttcaccagct ttttcacgag tgacattcat ctgggcgata ttgataccat
     1801 agcgggaaag cgcctctgta acaagggcaa tcatacctgg aatatcttga tgaacgatga
     1861 tgatagtcgg tgtattcata ttgagagaga cggcaaaacc attgagttcg gttacctgaa
     1921 tatttcctcc accgatagaa ataccagtca cgctgatggt cttgtgggca tttttaacag
     1981 taattttagt ggtgttaggg tgaggggcat tgctgtcttt ctgaatggtc cagacaatct
     2041 tgataccacg cttgtgggca atttccagac tatttggaat ttcaggatca tctgtatcca
     2101 ttcctaaaat acctgcaaca agggctaggt ctgttccgtg accacgatag gtcttggcaa
     2161 atgagttaaa aagttggaat tcaacttctg tcggagtatc atcaaaaatg gaagagacaa
     2221 tcttcccaat acgaacagca ccagcggtat ggctactaga tgggccaatc ataactggtc
     2281 cgatgatatc aaagacagat tgaaaacgaa gtgatttcat cagtttcccc ttataaaaat
     2341 tcttatctct attatatcaa agaatgaggg gcttggcttt aattgtggat gaaaaccttt
     2401 ctaatacctc aaatagcata aaaatagtat cttttatgac aaaaaacacc ttatttaggg
     2461 aaataaaaaa taattttgta atatttctac ataaaagtgt caagaaacag taatatttaa
     2521 agggtatgat agaactatag aaagaaggag aattttcgaa tatgaaatca ataactaaaa
     2581 agattaaagc aactcttgca ggagtagctg ccttgtttgc agtattttct ccatcatttg
     2641 tatctgctca agaatcatca acttacactg ttaaagaagg tgatacactt tcagaaatcg
     2701 ctgaaactca caacacaaca gttgaaaaat tggcagaaaa caaccacatt gataacattc
     2761 atttgattta tgttgatcaa gagttggtta tcgatggccc tgtagcgcct gttgcaacac
     2821 cagcgccagc tacttatgcg gcaccagccg ctcaagatga aactgtttca gctccagtag
     2881 taagtgaaac agttgtttca actgtaagcg gatctgaagc agaagccaaa gaatggatcg
     2941 ctcaaaaaga atcaggtggt agctatacag ctacaaatgg acgttatatc ggacgttacc
     3001 aattaacaga ttcatacctg aacggtgact actcagctga aaaccaagaa cgtgtagcag
     3061 atgcctacgt tgcaggacgt tacggttcat ggactgctgc taaaaacttc tggcttaaca
     3121 atggctggta ttaagaacta acgaacaaat aatatgtaaa gagtcgagga aatccctcgg
     3181 cttttttatc tttccttcgc ttggtaatag atctctactt cttttttgag atgagacagc
     3241 atagaagttt cctcggttag ctccagaagg gagaaccctt tttggataag tttagaatcg
     3301 tctgtacaaa tcccaatacg gacatagcag atagccagcc tatcgtagct tttcttattc
     3361 ttggcgtcct ccagtaaagt gtagcagatt tgcttacaca tgtttttatc ttgattgtat
     3421 agatagagtg tggaaaggtt taatagaatt gtcattcgca agtcatataa atgttgatag
     3481 tttttataga cttctaggcg ttgcaatatt tttccagtga tgagatggag gtattcaatg
     3541 ggaaaactga aaagaatggt attgaggatt tttagatcac tctcatacca tgtatcttgt
     3601 ttttcaattt ttttccaaag ttttttgaca acctgttctg cgtgttttga tagttcccct
     3661 gccccgtgtt gacggagata aataacaact tcaagcatat ctctgatttc ttctataggg
     3721 agatcgtggt gggtcttgag gtagtcttgg catttttgaa ataaattgac aagatcgctg
     3781 gtcccgataa tggaacgcat attgagatag gtttgcataa tctctgtgcg ttgacttggt
     3841 tgataaagtt gacagatata ttcaaactct tcaaaactca tattgatttg acggagaaga
     3901 aattccatat tttcatattt tggggttgtc ttgccacttt cgatttttga taagctggtt
     3961 cttgagagaa cacccccaca gacctcttct tgggtcagcc cctttgactc acgtatttct
     4021 ttatagacct ttccgaaatc ataacgcatg attttctcct ttctgtgaaa aaagttaaca
     4081 aacaataaaa ttttgttaaa aatattgtat catagcaatg taaaaagtaa aaaagaaagt
     4141 cgaaataaat ttgtgaaaaa agttaacgta attttaaaat gagcttaata agaggtatga
     4201 tgtaggtaga aaataaagat aggagaaaaa gtatgaataa aaaaatattt tgtattctag
     4261 tatgtattat atcattgatt agtctggcca taatctttcc atggggctgg tcgatataat
     4321 gtaagggagc ttgagttgtt aaaggaggct tagtccttgg acaaaaatct gctgtactac
     4381 aatgcttgga aagatatcaa gaacaaagaa tgatacgatt gaaaataaac tgattcaaga
     4441 actactgggc agtgattgag gtttgggtga catcaaagaa aagttagatt aattctaaag
     4501 aattgagtat ttctatgtta aaaaaatatc cttgtacgat gcaacatgat cagtcagatt
     4561 gtgctgcggc agttgtttca acagttcttt tatcttacaa aaaggaacta tcaatcatga
     4621 agattcggga aattattggt acagacatgt atggaacgac tgtcagtggt attgtttcag
     4681 gtctgaataa gttgaatttt acagtaaaag ctgttcgagt agcactggaa gatttgactc
     4741 caaaattaac atttcctgcg attcttcaag ttaagaatga tttaggtcaa aatcattttg
     4801 tggtattaca tagtataaag gagaaaataa atggaactcg tattaccaaa taattatgtt
     4861 gtgattgatg aagaagagat gatgtacctt gatggggggg ctatatatat acccaggtgg
     4921 gcaattacag gagccattac tggtgcagca tatgcagcat tagcagcagc aggaggtgga
     4981 ggccttcaac tagttcttgc atcttatgga ttacgctccg cactggtagc tgggattgtt
     5041 aaaggtttag gagtattagg aattcatatt ggaaatgctt ttgcaaatac tgttattaga
     5101 agtattgcat ctgctggaat tggtgctgga gctgattgga ttttaccaat attattgatg
     5161 gctgggatgg gcgacgtgat aatcaattga gaataggata atagtattat gatggttgtt
     5221 ttaatttcag ttattgttgc attcctgtac cataaaatta cagagtatgt caacaacagg
     5281 aaaattgata ttgttatttt tctatcattg ctgatttctc attttatttg gaattactat
     5341 tttttaccaa ttgaaatatt gatagcaatc agtggagcaa atttattatt aagtttttta
     5401 aaaaaattac agattaaata atattattcc atatgacata tcaactaggt cattagattt
     5461 tattgaagat ataaattaaa aacgaggttg ggaaaaaact caatttgaaa gtgaaattga
     5521 gtaaattttt ctattatata acgcatatta tcaagatttt tgaacacctg ataatatgca
     5581 cttttcttat tttaaagatt ttttgccttt cttcatttct tgaatgaatt ggagagatga
     5641 ctttatttag gtgtttgaca ttactataga taggatttag cagtactaat atgaactcat
     5701 attataggtg cagacttaaa ttttggaagc aagctaaagg atatcttatc taggtaacta
     5761 agtgctataa acaaagtcaa cgatcacttg ttaggaaacg atcctaacta tgcttaataa
     5821 aaaaggagaa aataaatgga actcgtatta ccaaataatt atgttgttct tgagcaagaa
     5881 gagatgatgt atcttgatgg gggtggtgtt ggttgtaact ggtggaacaa acgtgataat
     5941 attgctgcag ctgcagtttc tggagggatg tcagtatacc agacatatgc cattaaaaag
     6001 ttaattgcta atcgtggtat gatgagccga gtcgttagaa gtgctataac aagatatatt
     6061 ggtggagtta gtatggcaac tattactaat gctcttaata ttgcggctac tgttgctgaa
     6121 gttttctcat taggaggagc cattgcatat ggtttagata tcgtagacgg taaatttgat
     6181 ggatataata aaataaatct aataaattct attattatag ctcttggtat gatattggca
     6241 ttaattttgg tattatcaag agtgtataga aaatattttt atagagacga tgatgactaa
     6301 agtttttatc aataatttgg gctccttgtc aactgtagtg gattgaagaa aagctaacgt
     6361 ctggggaagg gtttaaagct ttgctttatc aaatattgac ttacagagtt gctctattgt
     6421 attgatgtat tcttagtaaa taatatggtg ttttgaatga aagatgatca gaaatattta
     6481 ttagcgggac tttattcgct tctagttgca atattttatt ttccattgat tgagtccaag
     6541 gggatttttg taagtatatt gatggcggtt ttacttttat atttaatata cttcattgca
     6601 acagtcattc atattgtgat aattaagttt ataagaaaaa aatcttttaa gtatttggtt
     6661 ctatatccat ttacttatga tgggagctgg agatttcaac ctataaactt actctacttt
     6721 ccagaaatgg tgagagatgt cattccgatt aatttagttc aagaatattg ccaaggacaa
     6781 ccttacggac tgttaaaaaa gatgctgaaa agaattcgat tgtcacgaga gatagcttta
     6841 ctcctggcta ccattatcgt ctattttttt acgcatagaa ttctacctct ttcagtattt
     6901 acgttcatat tttcttacat attgctattt gcacaatctt atttgggtgg taataccgtt
     6961 tggattggaa atagaagatt aataatagat gatgaatttg aaaaaatctt gctttcaaaa
     7021 agttatataa aagaaatatc atctgcacga tactcggaat acttgacatg tgaatacaaa
     7081 aaccttacac caatcatatt gttagctatt tttgaaaatt tgcttgactc ctacttgctt
     7141 cagaatcaga gtgaggttga cttagatatt ttttataaag ttttaccttt gctttataaa
     7201 gaaaaatata ctatgggatt caattacttt gtaagtttaa attatttact atataaggtt
     7261 ggatttttgg gaatcattta cgataatgaa gcattaggag atttgtcaaa acaatattta
     7321 aacagaaata tttcggaatt acaagatggt tctttcgatg gcggtataca agatgctgtt
     7381 gcctctaaac aaatagtagt tataaatgaa tttatagctt gtttaaattc cagatgcatg
     7441 ccatcccaat atgatagatt tttttataaa gatagacctt atattttttc cagaaagagt
     7501 cctattaagg ggtgaaaatt tgttacattt ggattaacaa gtgtcctagg gattagaaat
     7561 aactctagta tagctagata tttttgagta actagaaata tcattaggct acccatatga
     7621 ttgccagaaa agatggctga tgaatcttat ttatactgtt gcagtaggca ttgtctagga
     7681 taaataagct atcttgatgg gggaaattgg aataatttta gtagaaacat taaaggatta
     7741 tggacaagta gcgcaggatt aggtgatgca atgtgctaag ttggcttggt gttagcagta
     7801 gtattattta acaatggaaa gtagactaaa tgaaaaataa gcgttatttt tttgacacaa
     7861 ttttaatgat tttactgctt ataagcacga tattttgtgt cagcccagta tttataaaat
     7921 tggacatctt aggaaccccc tctcatgcca tcttaacctt tgtattggcg ataccgttat
     7981 tctatatttt gtctcaatgc ttacatactt tgctattact agtttcctca atcttttgta
     8041 aattaagacc aatttatttt tattttatat ttgtgatcat tataggagct aggaaatact
     8101 atcgaattct ctttcaccaa ctaatgggat ttagtccagg aatagcagtg ttttataagg
     8161 agagtcaaac aactaagaat ctgtttaaat tttactactt cttgtacttt acaacactaa
     8221 tttcatacta ttttttcttt acttttgtgt atgacaaacc gttgcttttg cctcttattc
     8281 cattttctat tatcatagca ttagtacaaa aactttatag aattgagaat caacaattat
     8341 ttctgttaaa atcaaaggta ctaacaatac tggaatctaa gaagaattgt gagtttaatc
     8401 ttcaggatta tcatgaaatt tggaaattgc aatcaaaatc tgagttgcca tgcgtggctt
     8461 tatcttatat cagtttgatc aagccatatc tttcagagag cgtgcgtgaa caaattgatt
     8521 tattagaagt taagcgattt aaaaaaataa atcatccaat atcactttat ggtatgttgg
     8581 atgttataaa attaaatcta tatttacggc attataatga aaaaaataaa tatgaatcaa
     8641 tgttaaagaa aattttagag gtaagaccag actttgtttt aatcgagcag aatattgatg
     8701 atagtttaaa tagctcgcaa ccattatctt tatctttagc catttcagaa atccaacttt
     8761 tgcttgaagt gtatatgggg atcaaacatg tttcaaccaa gtgataaaac cagaataata
     8821 tttgccatta taggggcaag ttgcatatta gtcagtatgt atcttattcc acttattaaa
     8881 actgagaata aattttggga aatactttta tttatattag gtgtattgct aattggaatt
     8941 gcgttactaa gtcaaaaaaa ataaattttg aaatagtatt agtgagttcg caatcattta
     9001 ttgctagtgg tttagaactg ttaagtttca aacaataatt gattttaaac cttaggtaac
     9061 ctataaaaac aaataaggta gagaaaggat atcttatcta ggtagctaag tgataaaaac
     9121 acagtcgtct atcacttgtt aggaaactat cctaactatg cttaataaaa aaggagaaaa
     9181 taaatggaac tcgtattacc aaataattat gttgttcttg agcaagaaga gatgatgtat
     9241 cttgatgggg gattttctat tccgagatgg cctgttgcaa cagccattaa tatagctttt
     9301 aatggtgttt taggtggagg agcaatcagt ctagttagaa attatattcg taattatggt
     9361 ttgcggcgag ttacaagcgc aattgctgga gcagctgcaa gatatgttgg ggtacgagtt
     9421 gcaaatagag tggcaggatt tgcactgtct gctattaatg gatttgcagc ttggatgtca
     9481 attggcgatg ctattacaac aatctgggcc aacaatgatg taaataggag agacccaaat
     9541 ttaaacgcct tgtggtaact agatatgggt attaaaatgg aaaaaatttt tgttattatt
     9601 ttttttgtat gtctatttat atcatccata acttttttag cctatgattt tgttagcgaa
     9661 gaaataaaaa agttgattat ttggattaat gtcgtttttt tgattttaat catagcaatg
     9721 atgatttatc caaaattaag aaaatgataa ggacactgta aaattatgat acgtaaacta
     9781 attgttttct tactaatgct ccctcttttt gtcttgtggc tagagttgca tgtcctagtg
     9841 aataacttac tattgaatct agaatctccc cttgattttg tgattagtat gagtcttgct
     9901 ttctgtagtt tgattttatc aaaaatagtt ttagacatac tatacgcatt gaaagattta
     9961 tacaagaaag aaggtcttat taagattttt ccctttattt ttataagaag gaaaaaagtg
    10021 aatgtgagat tctcgcccta tttttcattc catcgtaaaa gtttgtcgcc tgatgattta
    10081 agatctagga ttatatggag ttttattctt gaaattgcta ttatcctagt atttatttta
    10141 aaaattcctt ttgcaatcat catgcttact acaatatttt tctggactat aatggatatc
    10201 aatcatctcg tttttaataa gacggaattt ctttttaatc aaaacaaatg gcaaaaagaa
    10261 gattcatttg aaagtaattt gcataagata ttgatggaca aaatacaaaa atcagaatta
    10321 agctattctg acttaatgtc ccttcttctc tatgatgcga tgaaccaatc caccttttta
    10381 acggatagtg aactttttga ggatattttg aaaaaaatcg aagattctca taacaccctt
    10441 ctgtgtacag gtcttgtcga gttactgctg tatgagattt ctattagtaa caataacaac
    10501 tggcaagaaa aggttgataa aattagaata cagctcatca gaatcaatca attagatttt
    10561 ttctattata caagttggtt aaggcaaaat tttgatttct gtatgaatag agagtatcat
    10621 aaaatgaaat ctcggaaatt attactgagt aataaaaaga tagtttaagt tcgtcctatt
    10681 tgtaaaacaa atgaaacaat ttcaactaag gaggagaaaa caaatggaac ttgtattacc
    10741 aaataattat gttgtgattg atgaagaagt aatgatgtat cttgatgggg gagcttattt
    10801 aagcaagcgt gcttgtcaag gaatttgcgc agcagcaggt gtattggcta cagcagcagg
    10861 gaaaatagct tactgtattg gatatggtgt tcttaataga ggttgtgata ttaacgagaa
    10921 cccttatcct tgggatggat tcatatctgc aacagtaaga tgagtaatgt agataagatt
    10981 cgaaaaatac acatcatcgt gtgttgggtg tatatatttt tatcattcag ggcgattata
    11041 aatgatacgg agtatttttt gcttattttt ttagctttta tctactcaat agtatcacta
    11101 cctctatatt ctgtgaaaaa taaaatagta tctatctgtc tagttataaa ttcgattctg
    11161 ttaatgagtt tccccatttt aatcaataag tttttcccag aaagtttttt gacttatacc
    11221 gtattaataa gtgtttttat cctagagtta gcaatctttc atttaattag gaaagatttt
    11281 gatattaaat tgactaacga atataagaaa attagtcagt ttagaagcaa ggtgtctcaa
    11341 tctccttgga taaaatattt agagatttct agttttatat taactatatt tccatctatt
    11401 ctttatggta aagttgataa tcatgtactg actcttatct ttttgataaa aatttgtgta
    11461 gatactacga taaaattttt attcatcaga ttatttgaca caagtacttt aatgaagagg
    11521 agaatatttt ttcttttcgc attggatgtt atagcttact tatttttagg atatctttta
    11581 gtgattcaaa aagcaggcta tttgttttcg gttttacttc ttttttctaa tttttcagtt
    11641 ccatttatca aagaaaagga atacgaatta tttaaaaata gcaagtaata gaaatatgga
    11701 ttaaatctta gaacggctta aaatcagata tgataaaggg aaatagtctt atctaggtaa
    11761 ctaagtgcta caaacacaat cgaggatcac ttgttaggat tacattttaa tcatgcttaa
    11821 taagaatgga gaattcctat ggggattgta ttactctgaa gtttcgaata ccttagttct
    11881 cgtgattttg agctattcta gctttagaat ccttcagaac gtaaaatttt aagcagtcaa
    11941 agacttagaa tagcctgaaa acatttattt tatagcaatt ctaggcttag aattgctcat
    12001 atatatgtat ttgagctgct cgatagttag gacagctcta ttctgagaaa tctgagctat
    12061 caatgcttca gaatagtatt tcgccaatgg tttttattga agaggtgatt gtgttatgat
    12121 agtctaaaat aaaattgatt taatagagta aatggagaat cgttacatga aacatttttt
    12181 tgcaggaatt ggtgagataa aacttgttgg ctacttattg tatatatgtg taggactagc
    12241 ccctcttttt catatatatg tcataggttc ggaactgagt tttgtgaaaa tagtgttatc
    12301 tattttggga gttatatttg taagtatgtt aactattgct cgaatttatc ggacttttct
    12361 ataaagaata gtttggactt aaactgtatc taaaaacaga ggttggtcga ttatcagcct
    12421 ctttcttgtc gttgaaaaga gtgagatata ttgacttttg aaaaaaagat gtcataattg
    12481 agttgggata aacctaaaag taatatagta gattgaatct ggaatagtcc gttatgactg
    12541 ttaaaatatt tctagaaatt aatttgactt tcctaatcga tttgttcaca tcttgtttca
    12601 atctactata ctttctcgtt aaatcgttcg gcaattcatg gtgacatgga cacatagtag
    12661 taagcacact atgtggcttg gcagagctaa aaactgttcc cttgcgataa gcctaataag
    12721 cacaacatag ggagttagag aagccgactc taatcaacca ctttgggcaa tagtgagaac
    12781 tgccccgtgc ttttttattt tgagaaaata tggagtttgt cgttgaaatt acttgattgt
    12841 attttggact atcaagaaag attcaatgga aaaacatgtc aagtatcaac gaattataag
    12901 tatttagaga ttttcaaagt gaatttttgt ttgactgatt tacatcattt atttgactta
    12961 tacaaaatca cacgagatta tgctagtcaa acaaaacctg ctattcaaga tggtgttttt
    13021 attttagaag attttagaaa tatcctatgt acaatgatgt aatcgaaagg atatctttat
    13081 gtgagtttat cggcgatatc ttctatatct aagctagcaa gttgttgcat cgtagctaag
    13141 gatttatcta aaaatactat gaaattggac gtcatatttt ttgaggataa aaataaaaga
    13201 tccgcagttt taggtttacg aagagacaaa agcggagtat ttaaaccagt tactctacat
    13261 tttacaagcg ctaagaaata tgctaaagtt cgtaaaacag atgtgaaaga aatgaaatgg
    13321 ctataaagat tgtccgcagg cttaagcttg cgttttttgt tgtttaacga aattaaaaaa
    13381 caagctctct catcggaaat gtttttatag gaatgaagga agatgatgct atgtttaaaa
    13441 agagaatcga aaagggcaaa agtagtgtct ttatcttttt ggagtagaag cttatgatat
    13501 agaaatttgt aataaaaatg taatataaaa catttgacaa atatttacgg ggggagtata
    13561 cttaatataa gtatagtcta aaaatgacta tcagaaaaga ggtaaattta gatgaataag
    13621 aaaaaaatga ttttaacaag tctagccagc gtcgctatct taggggctgg ttttgttacg
    13681 tctcaaccta cttttgtaag agcagaagaa gctcccgtag ctagtcagtc taaagctgag
    13741 aaagactatg atgcagcagt gaaaaaatat gaagctgcta agaaggaata tgaggacgga
    13801 aaagctgctc agaaaaagta tgaagatgat cagaagaaaa ctgaggagaa agcggaagaa
    13861 gaaagaaaag cttctgaaga agaacaagct gcaaatctga aatatcaaca agagttggtt
    13921 aaatatatac gtgaaaatga tccaacaaaa aaagctgaag ctaagaaagc aatggatgag
    13981 gctgagaaag agtataagaa aaaacaaacg gaatttgctg aagttcgtgc aaaggtaatt
    14041 cctagcgcgg aagaattaaa aaagactaga caaaaagcag aagaggctaa agcaaaagaa
    14101 gcagaactta ctaaaaaagt agaagaagct gagaaaaaag ttactgaagc caaacaaaaa
    14161 gtggatgcag aacatgctga agaagtcgct cctcaagcta aaatcgctga attggaacat
    14221 gaagttcaga aactagaaaa agctctcaaa gagattgatg agtctgattc agaagattat
    14281 gttaaagaag gtctccgtgc tcctcttcaa tttgaattgg atgttaagca agctaaacta
    14341 tcaaaacttg aagagttgag tgataagatt gatgagttag acgctgaaat tgcaaaactt
    14401 gaaaaagatg tagaagattt caaaaactca gacggtgagc aagctggaca ataccttgct
    14461 gcagctgaag aagacttagt tgctaaaaaa gctgaattag aaaaaactga agctgacctt
    14521 aagaaagcag ttaatgagcc agaaaaacca gctgaagaaa ctccagctcc agcaccaaaa
    14581 ccagagcaac cagctgaaca accaaaacca gcgccggctc ctcaaccaga aaaaccagct
    14641 gaagagcctg agaatccagc tccagcacca caaccagaga agtcagcaga tcaacaagct
    14701 gaagaagact atgctcgtag atcagaagaa gaatataatc gcttgaccca acagcaaccg
    14761 ccaaaagcag aaaaaccagc tcctgcacca caaccaggtt ggaaacaaga aaacggtatg
    14821 tggtacttct acaatactga tggttcaatg gctacaggtt ggctacaaaa caacggttca
    14881 tggtactacc tcaacgctaa tggtgctatg gctacaggtt ggctccaata caacgattca
    14941 tggtattacc taa
//
LOCUS       DAVBCN010000050        14935 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVBCN010000050 DAVBCN010000000
VERSION     DAVBCN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14935
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.050"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(72..620)
                     /locus_tag="VQ497_001428"
     CDS             complement(72..620)
                     /locus_tag="VQ497_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HET2517726.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLVGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(635..1483)
                     /locus_tag="VQ497_001429"
     CDS             complement(635..1483)
                     /locus_tag="VQ497_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HET2517727.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            1671..3020
                     /gene="trkA"
                     /locus_tag="VQ497_001430"
     CDS             1671..3020
                     /gene="trkA"
                     /locus_tag="VQ497_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HET2517728.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            3024..4463
                     /locus_tag="VQ497_001431"
     CDS             3024..4463
                     /locus_tag="VQ497_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET2517729.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVVVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(4589..6268)
                     /locus_tag="VQ497_001432"
     CDS             complement(4589..6268)
                     /locus_tag="VQ497_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2517730.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGVIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSAMLAAICGYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSTFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QSISFVFLSLILVVDILIYLPFCRAYDRQLLMKEDVASSNDIILEEETSEIMPGELDE
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKSPSKSLQFVLEHYQAV"
     gene            complement(6338..7750)
                     /gene="lacG"
                     /locus_tag="VQ497_001433"
     CDS             complement(6338..7750)
                     /gene="lacG"
                     /locus_tag="VQ497_001433"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HET2517731.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGINMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGEPNILAVRYYHR
                     VFQSCLKYNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSDDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            complement(7760..8104)
                     /locus_tag="VQ497_001434"
     CDS             complement(7760..8104)
                     /locus_tag="VQ497_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2517732.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            complement(8272..10158)
                     /locus_tag="VQ497_001435"
     CDS             complement(8272..10158)
                     /locus_tag="VQ497_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HET2517733.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYMNRVFKTNIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRRHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEKFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYMRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGQMLWKKTRSYEELIEEYFFALYGENWQSVVEYLEKL
                     SIYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            complement(10160..11482)
                     /locus_tag="VQ497_001436"
     CDS             complement(10160..11482)
                     /locus_tag="VQ497_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2517734.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEAFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            11650..12873
                     /locus_tag="VQ497_001437"
     CDS             11650..12873
                     /locus_tag="VQ497_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET2517735.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            13172..13342
                     /locus_tag="VQ497_001438"
     CDS             13172..13342
                     /locus_tag="VQ497_001438"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2517736.1"
                     /translation="MTKQIPKFTKDTAQLYTCKWLLYNKVTKMYDHTIVNHSVREYIT
                     DSISTNTIEEAE"
     gene            13954..14238
                     /locus_tag="VQ497_001439"
     CDS             13954..14238
                     /locus_tag="VQ497_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517737.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            14231..14469
                     /locus_tag="VQ497_001440"
                     /pseudo
     CDS             14231..14469
                     /locus_tag="VQ497_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14565..14783
                     /locus_tag="VQ497_001441"
     CDS             14565..14783
                     /locus_tag="VQ497_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517738.1"
                     /translation="MMSIREQDLKDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
BASE COUNT         4906 a         3089 c         2390 g         4550 t
ORIGIN      
        1 acaatttcaa aacactgttt tgagcaacct gcggctaact tcctagtttg ctctttgatt
       61 ttcattgagt attagtcctt tttcaaactt cctgcacgag tttgggttcc tgcataggca
      121 agtaagagaa gagttcctgc aatagctaca gatacaccat tggcaattcc cgcaacaatt
      181 ccttgtgcaa atactttttc tgccgcttct tgataaatca caacatctcc aagtggtgcc
      241 aagacacccc aaacaagggc atttgcaagt agttgaatga ggttaaaaat aagaatatct
      301 ttccagtcaa aaacaccatt gatcacgcga acgtactttc taaaaagtcc cacaactaaa
      361 ccaaagagtc cgctagcgat aatccaagtc caccatagac cataaccaac aagagagtcc
      421 ttgattgcat gaccaatcaa cccaacaagc aaaccgataa tcggtccaaa aataatagaa
      481 agtagcgctt gtaccgcata ctgaagctgg atgcttgtat ttggaacagg ggttggaatg
      541 ttgatcatcc cgatgacgac aaagagggca gcgccaattc cgacagcaac aacttgttta
      601 attgtaaatt tgatttccat actattctcc tattttatcc ttctattttc tttatttcaa
      661 tggtccaaga tgaaccgaca cctacattat aggccttggc aaaggaacct tggttgatag
      721 ccaaacctaa acgatagaga gagttgatgt aaaggatggg ttgcccaatt ctcacatctg
      781 caaatgattt gccatagaca acctgatttt gatagaccag catatcagca tgatagatgg
      841 tcacttcaaa acgatcacca aattctggtt ccaacttgta aaattcttcc cgtgtgatag
      901 aggtccaaag cgaaccgaaa cgcacatcca gaatatcaat ggctcccttc accagatgat
      961 cttctatgat ggtcgctacg actggaagct ctacaatctg ttccacactg agctctggcc
     1021 ctacttcctc aaaagtaatg tgaccactgg ccagtttagc accagtatag gcatagacat
     1081 cacgaccgtg gaaggtataa gaatgctctg tgttttgacg cctattggcc acctcagaaa
     1141 tctcacgaat ggctacaatg ccaacgtgtt tcttgataaa ggaaagcgtc ccattatctg
     1201 gcgtgacaat gtattgattt tttgcagtct tggcaactac actcttacgt ttcgaaccga
     1261 cacctggatc gacaaccgat acaaacgtcg ttccctcagg ccagtaatcc accgtctgaa
     1321 agagacgata gctcccctca aaaatattat aaggcgtgat atcgtgcgtc aagtgatgga
     1381 tttttaaggt tggagactct tctaaagcca ctccaatcat agccgatacc gcaccatcaa
     1441 ccagaccaaa gtctgattgt aataccagta aattattatt cattttatct ccttgtatat
     1501 cctttatcat accatatttc agagaaagtc gctaatagct atttcaattg gttagggtat
     1561 agttttatct tatagcgaat tttcactaaa agctcttgtt attagctagt aggtgcattt
     1621 tttcttgaaa aagggtatga tagttacatc atgaaaaagt aggttttatt atgaaaatta
     1681 tccttgtcgg agggggaaaa gttggttttg ccctctgtcg ctccttggtt gcagaaaagc
     1741 atgatgtttt gctgattgag caagacgaag ctgttctcaa tcatattgtc agtcgctttg
     1801 atatcattgg tatccttggt aacggggccg attttgccat tcttgagcaa gccagcgtcc
     1861 aagattgtga tatctttatc gccctgactg agcacgatga agtcaacatg attgcagcag
     1921 ttctagccaa gaaaatggga gctaaagaaa ctatcgttcg ggtgcggaac cctgaatatt
     1981 ctaactctta tttcaaggaa aagaatattc tcggtttttc tcttatcgtt aatcctgagc
     2041 tcttggctgc ccgtgctatc gcgaatatca ttgacttccc caacgccctg tctgtcgaac
     2101 gctttgctgg tggacgcgtt agcctcatgg aatttgtcgt caagtccacc agcggtcttt
     2161 gccaaatgcc catttctgat tttcgtaaaa aatttggtaa tgtcattgtc tgtgcgatag
     2221 agagggatca tcaaattatc attccaagcg gtgacatgac tgtacaggat aaagatagaa
     2281 tctttgtcac tggtaaccgt gtcgatatga tactcttcca taattatttt aaatcacgcg
     2341 ccgtgaagag ccttctcatc gttggggcag gtagaattgc ctattatcta cttggtattc
     2401 tcaaagatag tcgtatcgat acaaaagtca ttgaaatcaa tcctgaaatc gccagcttct
     2461 ttagcgagaa attcccaaat ctctacatcg ttcaaggaga tggaaccgca aaagatatcc
     2521 tgctggaaga aagtgctcaa cactatgatg ccgttgcgac tctaacagga gtcgatgagg
     2581 aaaatctgat tacatctatg ttccttgaca gggtaggtgt acagaaaaat attactaagg
     2641 tcaatcgtac cagtctcctc gagattatca atgcgcctga tttttcaagt atcatcacac
     2701 ctaaaagcat cgctgtagat acgattatgc actttattcg tggtcgagtt aatgcccagt
     2761 attcagacct tcaagccatg caccatctag ccaatggcca aatcgaaacc ctgcaattcc
     2821 atatcaagga agccaataaa atgactgcca aacctctttc tcaactgaaa ttgaaaaaag
     2881 gggttcttat tgcagccatt attcgaaagg gcaagactat tttcccaact ggggaggata
     2941 tgttggaagt tggagacaag ctcctagtaa caaccttgtt gccaaacatc accaagattt
     3001 atgacttgat tgcgaggtaa gaaatgaata aaagtatgat tcgttacctc ctttcaaagt
     3061 tacttttgat tgaagctgtt ctcctcttgg ttcctgtggc tatcgctgtc tattaccgtg
     3121 aatcgagcca agtctttaca gccctctttt cgaccatagg gattctcgta ttgttaggcg
     3181 gttcagggag tttacagaag ccaaaaaatc aacggattta tgccaaggag ggagtcttga
     3241 tcgttgccct ctgttggatc ctttggtctt tctttggtgg tctccccttt gtcttttctg
     3301 ggcaaatccc cagccttatt gatgcctttt ttgagattag ttccggcttt acgactactg
     3361 gagcaagtat tttgaacgac gtttcagttc tcagtcgttc cctcctcttc tggcgaagtt
     3421 ttacccactt gattggaggg atgggagtgc ttgtttttgc acttgctatt atggacaatg
     3481 ccaaaaatag ccacctagag gtgatgaagg ctgaggttcc aggtcctgtt tttggcaagg
     3541 ttgtatccaa actaaaaaac actgcccaga ttctctatct cctttatcta gctctcttct
     3601 ccctctttgt catcatctat tatctagctg gtatgcctct ctacgatagt tttgtcattg
     3661 ctatggggac agcgggaact ggaggcttta ccgtctataa cgacggaatt gcccactatg
     3721 gcagctcact gattacctat ctggtcagta tcggagttct ggtttttgga gtaaatttca
     3781 acctctacta ctacctcatg ctccgtcgca tcaaggcctt ctttggagat gaagagctta
     3841 gggcttactt ggtcattgta gtggtttcta caggcttgat tagcctcaac accctctacc
     3901 tctacccagg gttttcaaag agctttgaaa tgaccttctt ccaggtttcc aacatcatta
     3961 caacaactgg ttttggatat ggagatatta ccaactggcc cctcttctcc cagtttatcc
     4021 tccttttcct catggcaatc ggtggctctg ctggatcaac tgcaggtgga ctcaagatta
     4081 ttcgaggcct catcctttca aaaattgcca aaaaccaaat tttgtcaatc ctatcgcccc
     4141 accgtgtttt gaccctccat gttaataaaa cggtgattga caaagatacc cagcataaga
     4201 ttctcaagta ctttgtcatc tatgctatga ttttgctagc gcttatcttt attgtcagcc
     4261 tagatagcaa tgattttctg gtcgtgacca gcgctgtctt tagctgtttc aataatatcg
     4321 ggcctattct aggaaccact tctagtttct caatctttag tcccatctca aaaattctcc
     4381 tatcctttgc aatgattgca gggcgcttgg agatttaccc aatcctcctt ctctttatga
     4441 agagaacttg gtctaagaga taaaatacaa aatccctctt tttacaattg tgaaagaggg
     4501 attcttatat taaaaaaggg ggaacgtttt taaatccaag cctatctgta agcaaaatcc
     4561 ttactcaaat aaaagatgga aaaacttact acacagcttg gtaatgctcc aatacaaatt
     4621 gtaaggattt acttggactc tttgttaaat gaatatattc cattcctctg gtagcaacta
     4681 tctgaatacc taatctttct gcatccacct tcatctctct ataataactc cgaacttgtg
     4741 gagccagaat aattaaatca taaacaccca taatatcata atgagctccg tacgctcctg
     4801 aattcgcaat cactctaact tctgctaatt gagcgccctc gttaattgca ttggctaatt
     4861 gcgcacttgt tccagaccct gcacaaagaa ccagtacttt caactcctta ctttttattt
     4921 catctaactc accaggcatt atttcacttg tctcctcctc taaaataata tcatttgagc
     4981 ttgcaacatc ttctttcatc agtaactgtc tatcatacgc tctacagaat ggcaaataaa
     5041 tcaatatgtc gacaactaaa atcaaagata aaaatacaaa ggagatagat tgaaaattcg
     5101 ttccaattaa caatcccaag ggaccaggaa aggcccaagg taactggata taaaatccat
     5161 tcattccaaa gaaatcaata aagacctttc ctagaaatac attcactggt ggagtcatca
     5221 aaaaaggtac aaaaagatag ggattcaaaa taactggcat accaaataga agaggttcat
     5281 tcaccgcaaa taaaacagga gtaattgtag ctttacctac cgattttaat tgtttagacc
     5341 gcataaagaa aatcaaaata aatggtacaa caaaggtagc ccccgttcct ccgatagcag
     5401 cgatataatt tccgaaatta ggcgttaaag aatgataagg aaactgccca tttgcaagaa
     5461 gttgagcatt ctcttccata ttgctcagtt gtaaagctgt aacagcaggt aagacaatag
     5521 atggtccatg aattccaaca aaccaaagca aagcgcacat aaaccaaatc aacatcatag
     5581 caggatatga ttctgccccc ttaaaaatag gagtcaatag ttgttgaaat acttgggcaa
     5641 aaggaacatc taaactaaac cgagatacaa tatctaacaa accactaata agtaaaacaa
     5701 aagaaaaagg aaaaatatct ctaaaagctt gtgatatagc cccaggaact tccttaggta
     5761 aatgaatagt aatgtctcgc ttaatacaga atcggtaaac atttacagta ataaaggtac
     5821 tgacaaacga agttatcaat ccttgagatc ctaaatagtt tgtcgacaaa cttgtagatc
     5881 ccgttttctc atcaactaca agcgttacag ttaatacaag atagccacat atggccgcca
     5941 acattgcaga agtatcattt attacctttc caggaggcat ttttctatta acatttccag
     6001 ccaatgactt tgcaactgtc cctgaaacaa taacacccaa aactcccatg gttagcatat
     6061 acactttcct catccaggca atagctggac tcggtaactc tactccgaaa tttttaggaa
     6121 tcataataat aatcatcaaa aagcttgaaa acataattaa aggcatagta gcaataaatc
     6181 catctttaat agccatcata taattattag tagaaatttt ttcaaatttc ccttgttgct
     6241 tttcaatggc agcgactaac ttatccataa aaactcctcc tgtaaaaaat ttaagctcat
     6301 agttaaaata tattaaacat cagtcaaatc gctaacctta attccgtttt aatcctttta
     6361 cccaaagagc acttttctta atatatctct tctgtgtttc ataatcaaca aagaaaagac
     6421 catatcgctt attgtagcca ttcgcccaag aaaattgatc ttgcaaagac catataaaat
     6481 agccttgaat attgactccc ctatctcgag cctctaaaac tttgtgtaaa tgttgctcaa
     6541 tataacggat tcttttactg tcatcaataa tatcattctc gccctctggc ttaacctctt
     6601 taagggctgt accattttca gttaaataaa tgaccggatg ttgaggatat tcttctttga
     6661 tacgaagcaa catatcaaat aacccttgag gataaatatt ccaatcccaa tctgttgtcg
     6721 gaataccagg cttttttaca aattcaccta gagcatttaa tttaaaagaa gactgtcctt
     6781 ttattcctgt tgaattaaat tcttgtctat tttcaccttt gtattcacga ataaattctg
     6841 aacgataata attcatccca aacatcgtat taagaggtgc agctcttttc ataatttcta
     6901 aatcaccatc ttcaataata aagctagaat tattagcttc caatatttga ttcaagtgaa
     6961 aaagcgtgtc atcactgtag tagcccaaaa aagttccatc taatagaaat ttattattat
     7021 aaacatcata ccgtttagct gccaaaatat tttctttttg cccatcaata ggatagcctg
     7081 gctttaaagc atgaatacaa cctacctttc cctcaatccc taattgatga aattcgagga
     7141 ctgcaagagc atgcgccaac aacatgttat gatttgcttg aattgcttca gataattgaa
     7201 aatggtgatt cggaggaaac tgacctccta tgtattggcc tgcagcaaga gacatcagtt
     7261 cattgattgt aaaccaatgc ttgacttctg taaattcttg gaaacaaaag cgagcatatc
     7321 gtatgaaacg atcaatattc tctctattca accaatcccc tgtttctaac attttctgag
     7381 gcgaatcaaa atgatgtaaa gaaacaaacg gaatcacatt atattttaag caggactgaa
     7441 agactctatg gtaataacga acagccaata tattcggctc cccatctata tcaggaaata
     7501 tacgaaccca agaaatagat aaacgcaaag cctgcaaacc atgttctgcc gccaaagcta
     7561 tatcctcttc gtaacgataa taaaaatcac tagctggatc tggtaagaac ggactatttt
     7621 cttgcaaata aacatcccac atatttattc ctttgccatc taccctagtt gcaccctcta
     7681 cttgataagc agcggtagca gctcctaata aaaactcctt cggtaaggct ttaacctgta
     7741 aattttccat gactaatcct cattgcaaac caatatgttc ttcaatcttt cgaattcgtt
     7801 catattcatc aataaaaaac tttacctgat catacaatag catcgttgtc atcaaagtat
     7861 cttgaccatg aatcatgata aaactcactt caaaattatc tccagatgcc tcctccgcta
     7921 ataaattagt ctgttctcga tgggcagaca ctattgagtc gtttgcagac tcaaccaatt
     7981 ctcttgcctg ttcaaaatat ccatctctcg caaacgccaa agcatccatc aaatctgacc
     8041 ttgcatctcc tgcgtaagcg acaattgtaa aaccaagcat atttatttct tcttttgatt
     8101 tcatctttct tcctcctcaa tcttgaacca tcttttacac atcattatta aaatgacttc
     8161 gctacgatac aaatagaaag tcgaccctaa atatcttaaa aatccttaaa tttcataata
     8221 tttaggatca atcttctctg aataccttgg ttcattttct agacttatga tttataagtg
     8281 gaaaccagcg taattttttg ctacttcaat tacacgataa acatccaaat tagattggaa
     8341 tagcagttcg tgcccacgga tataattcaa cacatttctc cattcatctt gagcttgcct
     8401 tgtttttcca gttgcttgaa gatataaagc cttcgccatc ttaacaacat attcacgatg
     8461 ataactgagc tgtttccatt catccttttg actatttaat aacttagaaa tattttcctc
     8521 aataattggt aaaaaattat cagctagatt gtaagctata taataatgat tcgctaaaac
     8581 atcattttga cgactgccaa ttgcattaaa atagtcacaa gaggaataaa tggataattt
     8641 ttctaaatat tcaacaacag actgccaatt ttccccatac aaagcaaaaa agtattcttc
     8701 aatcaattct tcataacttc ttgtcttctt ccagagcatt tgccccatga cataattagg
     8761 aaaattatga gggaatccgg cacgtaattc ttgacacgaa atgtacccat tcaaatgtag
     8821 gtttgaaaga taagatacat ctctgtaaat agtttgacta attttcatat agcctaaatc
     8881 gccataatga gcacgcccta aaggatagtc atatacgaaa ctatctcctt taaatgtttt
     8941 ttgccactca aaaagataag ataaattttc ctcaagagaa ttcggaagta taattttatt
     9001 acgcatataa ggtttaggcg taggtatgga attgtcaaaa tctacatctg cataactcat
     9061 ttcaaatgtt cttgtaatcg gtgcaaacat catggtaaag cgttcaggat tatctaattt
     9121 ttctttctga ggcgcccata acaactcatg gtaaagcaga aaacaaatct ttgtatctaa
     9181 tccctcactc gttaaagccc tatccaattg attgagaata cgaatatact gatccgaaac
     9241 caattcttgt ctacagtttt cgcattcaca aatattatta cgagcatccg acaaccatac
     9301 atgtaagtag ttaacatcag gtcttttctt ggcataatcc ttgataattt ctaccatctt
     9361 atcagctaca tctggatttg aaaaatccag gctggttaaa atcggagccg tattaaacaa
     9421 ttctcgtttc ccgtttattt cagcgacata gggtttcttc tcctctgaaa tactaagacc
     9481 tgattcccag ccaaattttg aagagtaacc taaaacctca cctgtccatc catgaccaac
     9541 acgatgatga ataagacctc ttttttgcaa ttctttatcc aacctatcac tcaattcttg
     9601 tactaattca tttgagaatt tttctttatt taggtatgga ttaaactcat gttcatacca
     9661 acgtttcaaa aaagagtaag gattttcaaa ctggatgaaa aaactgttca tcccaatctt
     9721 aggtagccaa tcaatgaaat ctagtatatt ttcaaatgaa tccgctccct ctatacatac
     9781 tcccctatga tagtaactgg ctgtttcatc tatagatagc tgtttatcta aaaaatcttc
     9841 aaatcgtaac tctggaacaa agtcatgtct gcgccctggt ctagtataca caaccccaaa
     9901 ttcatgaaac attcggtaaa ttccaataag taaacctaca attgtattag actcaattct
     9961 ccccttaccc ttgtctaatg agataagaac atggtctttt ctgtcctctt tattgactaa
    10021 tcctacaaaa atatccgctt ccttctcctc gacaagctct atgttagtct taaagacccg
    10081 attcatatag taacttaatt cctctaaggc aaagctagaa taagtatttt tctgttgatc
    10141 aaatactctg ataaccatct tatttccctc caacagtagc tttttctttt tcaagattgc
    10201 gtttatctgc aattttgaaa aatggataat aaattgctac agaaaccaaa atcaaaacaa
    10261 cttgtaatag agctcctcgc caactagctc ctgtcgcaag gaagcctcct atgatcggtg
    10321 gcattgtcca cggaaggatt acacctgttg tatagggtac taatcctaca gccattgata
    10381 cataggtaat caaggcattg attgtaggag tagcaataaa cggaatcagc ataatcggat
    10441 ttaaaactgt tggaaacgta aatagaatag ctgtattgat attaaaaata gacggtataa
    10501 tagctagctt acctaatgtt ttattcgcac gactcttact aaataggaag agacaaatcg
    10561 caagaccaat agtcgctcca ccgccaccaa taaatacaaa taaatcttta aacggtaatg
    10621 taataatgtg ttggagtgtt tgtcctgcag ccacagcttc ttggttttct gtagtaaatt
    10681 gtaaccaaac tgggtctaca aaagcattta aaacttgtcc cccattaact ccacagaacc
    10741 aaaagaatga gtttacaata acacatagaa tcatacctgg aagcgttcct gcaattaact
    10801 taagcggtgt tccaacaatt gcacctagga gtccgttgag acctcctgca actcctgccg
    10861 cttcaagacc ttttaagacc agagcccaca aaacaaaagt agtaaagcca ggtaaaagag
    10921 ctgaaaatga tttacttacc acatctggga caccacttgg catttttatc gtaattccgc
    10981 gctggataaa catacgataa atctctgctg taaccaatcc aatagtaatc gccataaaca
    11041 aagaagatgc attcaggcta gaaaatggta ttgcgccgcc aaataactgc ttgacctgct
    11101 ccccattttt atcataaacc atactcgaaa aacgaggtgc catcaataca aaactggata
    11161 aggctatgat ccctgccgac ggaccatctg taccatatcc ttccgacaac ctgtaggcaa
    11221 taccgaaaca agcgaccaag gagataatcg ctactgttga ctgattcata gttgtcaaga
    11281 tacttagcaa tccaacacta ttcagccaat ttgtaaaagc ctcttgaggc caactgatta
    11341 gaataagaaa taaagagccg actaaaatca aaggcattgc catcataaat ccttcacgaa
    11401 tagctttcaa atgccgttgg ttgccaactt tttcaccaaa cggagcgact tttgtttcta
    11461 aaaacgctaa aatattgttc atcaccggaa ctcatttttt tattttttat aaattaaata
    11521 taccactaac agaaagcgtt gtcaatatat tttttgttgt ttttacaata tttttttctt
    11581 tgtatagaat aataatttta attgattttt tcttatgttt aatataaaat aaaccaaagg
    11641 aggaaacaaa tgattacaaa acaatcagcc tttttacaaa atagagccac tatactggag
    11701 tttctatatc gaaatccagc aacatcccgt acagatattg ttaatgaaac aggacttact
    11761 ccagcaacaa cgactaacat cataaaagag ctaagcgaac agtccctcat atacgaaact
    11821 ggtgatgaat ttagcgagtt ttccggatct ggaagacgta gaaaaactat ttctatcaca
    11881 gataatattc cgtatgttgt tggaggtatt gaaataaacg tcctaggtat ctttctcagt
    11941 ctatgtgact tacaagggaa aactcttttc gagacagaaa ttttgaatga agattatcct
    12001 atttcagaaa tcaattccac cattaccaat atgataaaaa cagctataga gtacgtccct
    12061 ttggaaacaa aattacttgg atttggctta tcaatacctg gacattataa caaagactcc
    12121 ggaagtatca ttacaaacaa ccccatatgg gaatctttta atttattaaa tgtaattaaa
    12181 agattcaatt ttccttttat tgtaaaaaat aatatcgatt gtatggctat aggacaatac
    12241 ctttttaatc cacacaatac ccccgataac tttattttcc tacacgctgg attaggtatt
    12301 tacacttcct ttttcacaaa agaaaaaata ggagcctcta aaaatcctta tatcggagaa
    12361 attggacaca ccattgtcga attgaatggg caatattgtg aatgcggaaa aaaaggttgt
    12421 ttacaaacat atatttcgga tgcttggtta atcaaacatg cccaattatt atttaaaaat
    12481 tctcaactaa ctgtactaaa aagccttgta aagactgaaa aagacattca tttagatacc
    12541 cttttaacag cttacaactt aggcgactct gctttacgcc aacaaattga taaaggagtc
    12601 aatttattgg ccacttctat tgcaaatctt ctcctcatca atcctgctga taaaatctat
    12661 atcaacagtc aattgcttaa ttatcaacct ttcactcatg aagtcaggga taaaatccaa
    12721 gatcagctcc acttcgttcc ctttactcgt aatatagaaa ttgaaatttt accttacaac
    12781 aaacatcgtg gaagtatagg agcttgtgca ttagctatcg ttgctttttt catagagcat
    12841 agcaatgtat tacaagatat tatttcacct taatatatat tagaaatcta tagacctgtt
    12901 taaatcaact ataacctgta gtaaatatct cgtatttaga caatatgaaa acaagacgac
    12961 ttccatatag gaaaccgcct tctcgctatg ctgcatgaaa ctgtaaaaat agatgaaatt
    13021 gatagcggta gtcagatcaa gaaacatcgt aactctaaaa agtcacaata tataagttca
    13081 tcctcggaaa aatatcattc taattattga aatgcctaca tgaaaagaaa cgttaaatgc
    13141 tcataaaaca acgaatacag gtatcaaaac tatgacaaaa caaatcccta aatttactaa
    13201 agacactgct caactttaca cctgtaaatg gttgttgtat aataaagtta caaagatgta
    13261 cgaccacact attgtaaatc atagtgttcg cgaatatatt actgatagca tttctacaaa
    13321 tacaattgaa gaggctgaat aggctttaaa cgaaatatgt cagtgttttg gcattcctag
    13381 ccttcatatc atttaaagaa ttgtatagat aaaatttttt ccaatacaga cactcgtaac
    13441 aactgcttca tttttctacc aacatattta ggaacaggat aagatacaag aggattaatc
    13501 catagctcag ttctatacca atctaagaca aataagctaa aaaaacgatt gataataagc
    13561 aaatagattc caaattttct ctatctgctc attttaataa acaatactag tgtaactatc
    13621 cttccagtca gaagcttgtc aaatcacacc gaaaattctt ctaaaattta tctcgttagg
    13681 caatcaagca aaaactcgac gatagtacaa acattatcat acagaattga cttcctaaat
    13741 tatatacttt agtaaggttt tcggataaga aaaaaggttc attttacatt tctaaacatt
    13801 cttttctaag atgaaaaaca gaatttttcg attgtaattt aaagcaacaa gaagattttc
    13861 agtatcatcc tatagatacg agctaattaa gaaaaactac atttttgaat ataaactaca
    13921 ataatataaa ctaaattcca taggaggaag acaatggatt ggtacgatta tatgatacag
    13981 gcatccaaac aatcacaatt caacgcaagc cattggtttc gctatttgcg aaaagttatt
    14041 tttgaagact attcttattt aacaaaccaa gatgtagaaa agttgctaga ctccaaagaa
    14101 ctaacccgtt ttcaaaaaat tagcttgaag tatgcctttc aagagcatac tccaactcat
    14161 aaatatgtga tttcattaaa taaacctgct aagttaacca atgttcaaaa attgatggag
    14221 aaatacaaac atggataaaa tgaaaccggt cttccaagcc ctaaataaga aattaattca
    14281 ggaaaatctg actttaacaa ttatggttat gtcttagaat atcatggttt acgtgccaca
    14341 caagatgttg atgcttttta tgaccaaaat cagaaaatca atgaaattat tgctcgtgta
    14401 ggaaaacagt ttaatcttaa catccacgaa gagttatggc tcaacaatca tgttgcaaag
    14461 caaatatgaa caaacaacct cccttaagtt tatgcgaatc cttgtactca tttgagaatc
    14521 tgaccgtttt agtagtccct attgagtatg tgttaggaat gaagatgatg agtatcagag
    14581 agcaggattt gaaagatatt ggtgcaatta ttaaatacaa aaactttcat tcaccattcg
    14641 atacctttaa atatttaaaa gacatgggat ttgatactat tgacctctct gttctattag
    14701 aaggttttag ttatgcttat ggtatggatt ggttagaaaa attttttaaa gaaaatcaag
    14761 ataaacttcg cgaattttat taacagtatc aaacaagatg attgtcaatt agccagtcgt
    14821 cttgttttta tgatggaatg aaataaaatc ggaacaaatc acttagagaa ttccagacaa
    14881 tttctagcac tgtttgagac gtttcagcgg actatagcaa aatgaaataa gaata
//
LOCUS       DAVBCN010000051        14377 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVBCN010000051 DAVBCN010000000
VERSION     DAVBCN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14377
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.051"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(107..1396)
                     /locus_tag="VQ497_001442"
     CDS             complement(107..1396)
                     /locus_tag="VQ497_001442"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET2517739.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQTLRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(1474..2142)
                     /locus_tag="VQ497_001443"
     CDS             complement(1474..2142)
                     /locus_tag="VQ497_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2517740.1"
                     /translation="MSLSMSSADGENLKNEKLSIKDQFLTIYRNLRLVFLKSGQKNFG
                     FMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALFINQFCVLVAVIISSLTGND
                     YFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFFTLYVSGKVQPKISAMLMKN
                     LAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNMQLTWMLFVGLSLLAIFLTILNL
                     KNDI"
     gene            complement(2454..2720)
                     /locus_tag="VQ497_001444"
     CDS             complement(2454..2720)
                     /locus_tag="VQ497_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517741.1"
                     /translation="MKLLFRNQAYRLLTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTFFTVFAGIRADYTRDKVKWMVYSGLFQAVLFF"
     gene            complement(2910..3761)
                     /gene="rgg"
                     /locus_tag="VQ497_001445"
     CDS             complement(2910..3761)
                     /gene="rgg"
                     /locus_tag="VQ497_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HET2517742.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELLV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSYNKQKHILNAIIIKSYMKSIDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNFFHTILLNGFLASIECEKFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            3885..4226
                     /locus_tag="VQ497_001446"
     CDS             3885..4226
                     /locus_tag="VQ497_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET2517743.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIEDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            complement(4435..6138)
                     /gene="ilvD"
                     /locus_tag="VQ497_001447"
     CDS             complement(4435..6138)
                     /gene="ilvD"
                     /locus_tag="VQ497_001447"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET2517744.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(6371..7303)
                     /locus_tag="VQ497_001448"
     CDS             complement(6371..7303)
                     /locus_tag="VQ497_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HET2517745.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATNNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(7300..8157)
                     /locus_tag="VQ497_001449"
     CDS             complement(7300..8157)
                     /locus_tag="VQ497_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET2517746.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(8161..9507)
                     /locus_tag="VQ497_001450"
     CDS             complement(8161..9507)
                     /locus_tag="VQ497_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2517747.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMVIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSATEE"
     gene            complement(9520..9804)
                     /locus_tag="VQ497_001451"
     CDS             complement(9520..9804)
                     /locus_tag="VQ497_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2517748.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(9808..11838)
                     /locus_tag="VQ497_001452"
     CDS             complement(9808..11838)
                     /locus_tag="VQ497_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET2517749.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NSHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            12030..13037
                     /locus_tag="VQ497_001453"
     CDS             12030..13037
                     /locus_tag="VQ497_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET2517750.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            13040..13240
                     /locus_tag="VQ497_001454"
     CDS             13040..13240
                     /locus_tag="VQ497_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2517751.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            13392..13574
                     /gene="rpmF"
                     /locus_tag="VQ497_001455"
     CDS             13392..13574
                     /gene="rpmF"
                     /locus_tag="VQ497_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET2517752.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKITKAASAE"
     gene            13590..13739
                     /gene="rpmG"
                     /locus_tag="VQ497_001456"
     CDS             13590..13739
                     /gene="rpmG"
                     /locus_tag="VQ497_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2517753.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(14231..>14377)
                     /locus_tag="VQ497_001457"
     CDS             complement(14231..>14377)
                     /locus_tag="VQ497_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2517754.1"
                     /translation="YLNESGSMATGWVTVSGKWYYTYNSGDLLVNTTTPDGYRVNANG
                     EWVG"
BASE COUNT         4621 a         3045 c         2476 g         4235 t
ORIGIN      
        1 ataacttcaa aacagtgttt tgagctgact tcgtcagttc tatccataac ttcaaaacag
       61 tgttttgagc aacctgcggc tagcttccta gtttgctctt tgattttcat tgagtataaa
      121 atcctagttt ttcaaagatt tctgagaagt tttggctgat tgtctcaagt gacacttgca
      181 cttcttctcg ggtttggttg ttcttgaccg tcacttgtcc gctttcgact tcgctctctc
      241 ctagggtgat gagggtctta gccgcaaaga catctgctga cttgaactga gctttgagct
      301 tacggttgag gtaatcacgc tctgctttga aaccttgttg gcgaagagtt tggactagtt
      361 ctagggcttt gacatttgct ccatcaccca agactgcgat atagacatct agggcgtttt
      421 cgatagggag ggccacgcct tgtttttcaa ggatgagaag caggcgctct acaccaagcc
      481 caaaaccaaa tccagcagtt tcagggcctc caaagtaagc aaccaaacca tcgtagcgac
      541 cacccgcaca gactgtcaag tcattgccct caatctctgt gataaactcg aaaatggtgt
      601 ggttgtagta gtccagacca cgcaccatat tggtatcgat gatgtaatct actccaagat
      661 tttccaacat ctgacgcaca gcatcaaaat gagcttggct ttcttcatca agaaagtcca
      721 agatagacgg cgcattctct actgccacct tgtcttcttt ttccttagag tccaagacac
      781 gaagaggatt ttcctccaag cgacgttggc tatccttaga caaggtctcc ttgagcggtg
      841 tcaaatagtc aatcaaggct tggcggtagg ctgcacggct ctcaggattt ccaagagtgt
      901 tgaggtgcaa tttgacacct tgaataccga tttccttcaa aaaatgggct gccataacga
      961 ttgtttccac atcggtagct ggattgctag agccaaaaca ctcaacacca atctggtgga
     1021 attggcgcaa gcgccctgcc tgtggacgct cataacggaa cataggtccc atgtagtaga
     1081 acttgcttgg cttttgcact tctggggcga agagtttatt ttccacatag gaacggacaa
     1141 cgggcgcagt tccttctgga cggagggtaa tatgacggtc acccttgtca taaaaatcgt
     1201 acatttcctt ggttacgata tccgttgtat ctccgacaga gcgactgata acctcgtaat
     1261 gctcaaaaat aggcgtgcgc acttctgcgt agttgtagcg tttgaaaatt tcacgggcaa
     1321 agccctcaac gtactgccac ttggcagatt cagcaggtaa aatatcctgc gttccttttg
     1381 gtttttgtaa tttcataggg aatcctcttt aaacttaata gtcttatttt accataaata
     1441 gagggattaa aacagtgaga aaaaaattag gatttagata tcatttttga gattaagaat
     1501 tgttaaaaaa atagctagca aggaaagacc aacaaatagc atccaagtca actgcatgtt
     1561 ccatacggct actagtgaaa aacaagctgt tcccacaggt atggataagg taaacaatag
     1621 acctaaaaaa ttactagtac gagctagaac ctctggagct agatttttca tgagcatggc
     1681 actaatcttt ggttgaactt taccagacac atacagagta aagaagagaa atagcaaacc
     1741 aagcacgact tgattgaata aattagccaa accaactaga ctaagtccta cggtcgccca
     1801 catcatcaat ctaggcaagg actgcttccc aaaataatca ttgcccgtaa ggctactgat
     1861 gatgactgct actaaaacac agaattgatt gataaatagt gcctctgtat aagaaaaatt
     1921 caagagagaa tggctcaaaa agaagatatt ataaattcca cccaaagcgc cacccaagga
     1981 attaataagc aagacagcaa agagcataaa accaaagttt ttctgtccac ttttaagaaa
     2041 aacgagacgt aaatttcggt aaattgttag gaactggtct ttgatagaaa gcttctcatt
     2101 ttttaagttt tcaccatcag cagatgacat tgacaggctc aatttgcttt ttcctaaaaa
     2161 gaggatagtg gctgatacga ggaaaaagca agcattaatt cccgcaacga gggaaaaatt
     2221 gttgaccgat agagctaaga gccagactcc gaaagcttga ccaccaatag ctgaaatata
     2281 ggtgatgaac tgtgaaaaag aataagcctc catcagatca tcttcagcta ctttttcctt
     2341 aataagaggc atacgcaggc cacctgcaaa atcactgatg atatcactaa tgacattgat
     2401 caaacacagg ctagaaaagg caaagagact agcttgctga acaactaggg ctgctagaaa
     2461 aatagaaccg cctgaaacaa accgctatag accatccatt tgaccttgtc cctcgtgtaa
     2521 tctgcccgaa tccctgcaaa aactgtaaag aaggtcggaa gaatcatgac aatattcgcc
     2581 atagcaacag caaaagatgc ttgtgacaag gtcgatgcat agacgataaa gaccaggttg
     2641 aaaatcgaag caccaaaagc attgaagaag cgtgacaaag tcaataggcg ataagcttga
     2701 tttctaaaca atagtttcat agataatctc cctccttgtt ctaagttcta catcaagaat
     2761 agctttagta tagcacttat gaatttgcga aacgattctt ttgaatatat gcaacaaata
     2821 aaatgagcaa aaaacgatag aacttttgta atgacctgag aaagggcaac agttctatcg
     2881 cttcaaattt tttcttagtt tgcagatatt caacattcgg tcgtatttct atagtattcg
     2941 gcagatttat tacagccaag catctcaaaa atacggacag catcctccat ctttttctgg
     3001 ccttccttga ctctaccttg cttgctatca aggagacctt ctgcccacag ataaacaatt
     3061 cggaaatagg tctcattttc cttgtagaaa tgctcttcga taacacgttt aaaataatag
     3121 gcattggtaa atttttcaca ctcaatacta gctaaaaagc cattcaatag tatagtatga
     3181 aaaaagtttc gattaccaga catttccatt agaaaatcag atttacgtac catttctcgt
     3241 acatatctag taaaaagaga aacagataaa aatggagaac tgactgaaaa taaattgagt
     3301 tcatagattc cccagatctc ggtagaaaac aaataatcat gaaggacttt tccttcctct
     3361 gctgttaact ctaccctttc atctatgctc ttcatataag acttgataat aatggcattt
     3421 agaatatgtt tctgtttgtt gtaagaatgg gcatgctttt gatactccct gcgatataag
     3481 tcctcaagag gtgctatatt ctttggttcc aagacatctg taatttcttt tctcaactca
     3541 gaatctgtat catactggaa acctctcgcc agaaagagga tctcctccac actggcagat
     3601 atattttcca gagcaaatag aaacttttcc accaaaagct cactctgacc tgtttcaaaa
     3661 cgggacaaca tagacggcga aaattgtcct ccggttgctt gtctcagtga gatatttctt
     3721 gactctcgta attgtctaaa gacttttcca atctgctcca tagacttccc cttgattccg
     3781 tattttcttc attttatcat atttttcaga aaattcatca aaaacttgcc aaattgtcag
     3841 aattatgaga aaatagagga tatttatcac gtggagggac tgctatgaga gacgatatca
     3901 aaatcaatga ccgtgctttg gccttgcaag accaaattat cgaaaaacta gagaaagttt
     3961 ttgatacaga tgtggaattg gatgtttaca atctaggtct gatttatgaa atcaatctgg
     4021 atgaaacggg gctctgcaag attgtcatga ccttcaccga tactgcctgt gattgcgccg
     4081 aaagcctgcc tattgaaatc gtggcaggtc tgaaacaaat cgagggtatc gaagatatca
     4141 aggttgaagt tacctggtcg cctgcttgga aaatcacacg aatcagtcgc tatggccgta
     4201 ttgcccttgg actaccacct cgttaagcag gccaatcact tttaaagatg aaaaaagcaa
     4261 gccgaaaatg gcttgctttt tgatactcaa tgaaaatcaa agggcaaact agaaaactag
     4321 ccgcaggttg ctcaaaacac tgttttgaag ttatggatag aactgacgaa gtcagtaacc
     4381 atacctacgg caaggcgacg ttgacgtgat ttgaagagat tttcgagtat gagtttattt
     4441 tttacctgac ttgtccatat tccagaagtc tgtcacggct ccgcgtgaag cagatgatac
     4501 gatgtgggca tatttaccga ggacaccacg gctgtaaagt ggtggcaagg ttgtttctgc
     4561 cttgcgtttt tcaagttctt cttcggatac ggccatggaa atttctttgg tatcttggtc
     4621 aaccgtaacg atatcgccgg tacggagata ggcaattggt ccaccatcct gagcttcagg
     4681 agcgatatgt ccaacaacca gaccataagt accaccagag aaacggccgt ccgtcaagag
     4741 ggccacctta tctccctgac ctttaccaac aatcattgaa gaaagtgaca gcatctcagg
     4801 cataccagga ccacctttag gtccaacaaa acgaacaacg actacatcgc catcaacgat
     4861 ttcatctgtc agaacggcct gaatcgcatc ttcttctgag tcaaagacct tagctggccc
     4921 aacgtgacga cgcactttaa cacctgatac cttggcaact gcaccgtcag gagcaaggtt
     4981 cccgttcaag atgataagcg gaccatccgc acgttttgga ttttcaagtg gcatgataac
     5041 tttttggcct ggagtcaagt ctgcaaagtc agccaagttt tcagctacag tcttaccagt
     5101 acatgtgatg cgatctccgt gaaggaaacc atttgccaac aagtacttca taaccgcagg
     5161 gacaccaccg acttcgtaga ggtcttggaa gacatactga ccagatggtt tcaagtcggc
     5221 caagtgaggc acacgttctt gaatcgtatt gaagtcctca agtgacaagt caacatttgc
     5281 ggcatgggca atggcgagca agtgaagagt ggcgtttgta gaaccaccga gagccatcgt
     5341 tacagtgata gcatcttcaa aggcttcacg agtcaagata tctgatggtt tgagaccaag
     5401 ttccaacatc ttaacaacag cacggcctgc tgcttcgata tcttctttct tatcagctga
     5461 ttcagctggg tgagaggatg accctggcaa actcatccct agaacttcga tagcagttgc
     5521 catggtatta gcagtataca taccaccaca gccaccaggg ccagggcagg cattacattc
     5581 aagacgtttc acgtcctcag ctgtcatgtc accgtggttc cattttccga taccttcaaa
     5641 gacagaaacc aagtcgatat ctttaccatc aagatttccc ggtgcaatag ttccaccata
     5701 ggcgaaaata gctgggatat ccatattagc aatagcaatc atagatccag gcatgttctt
     5761 gtcacagcca ccgatagcga cgaaggcatc cacgttgtga ccactcatag ccgcctcgat
     5821 ggagtccgcg atgatgtcac gagatgttag agagaaacgc ataccaggcg ttcccatagc
     5881 gatcccgtcc gctacggtaa tggttccaaa ctgtacaggc caagcgcctg cagatttgac
     5941 accttcttta gccagtttcc cgaaatcatg caagtgaatg ttacatggtg tattttccgc
     6001 ccaagtcgaa atcactccca caatcgatgt ttcaaagtcc ttatctgtca taccagtcgc
     6061 acgaagcata gcacggttag gtgatttaac catgctgtca tagatgctac tgcggtgacg
     6121 tttatctaat tcagtcatct tatccctccc atttcagttt ttactattat agcacaattt
     6181 tcgcatgaag aacagaataa aattcttgaa ttttcagaaa attctataca catgtgaaat
     6241 atttaaaatt aaaaacaaca aagcggatta gtgcactttc tgatgaccag aatatgcttt
     6301 ttaatccgct ttctttaaat aacgtactgt aatttttaca gaaattcttt caaataagtg
     6361 tatttaacat ctatcttgca ttataaattt ctagaacctt ctcttttata ttcgattcac
     6421 tcaaaccata ctcattaaga agataatcca ttttccctac ttgaccgaat ctttcttgaa
     6481 cacccatccg atgaattttt gttattccat catcagagaa taattcacat aaagcactgc
     6541 caattccacc tatctgattg tggttttcta cagtaaatat agtttttcca cttaacattg
     6601 tttttatctg ttctggtatc ggtttgattc taaataaatc tatcacacct actgaataac
     6661 ctaatttaga cagttcatct gcaactcgaa tacttggagc aaccattatg ccagaagcaa
     6721 cgattacaag atcttcacca tgccttaact caatgtagcc tttagaaaaa tcttctccac
     6781 cttgatacac aggaactgga gcttttctaa ttgttcgaat atattttagt ccttttaagt
     6841 ctaatgtctg gttcaatatt tcacgaaatt ggatatcatc agttgcttcg aaaatgattg
     6901 atttaggaat taaacgtaac aatccaattt cttcaaatgg catatgtgtt ccaccattca
     6961 tctctgccgt tactcctgca tctgatccaa tcacagtggc atccaattgt gcgtatccaa
     7021 gagaaataaa taattgatca aatactcttc gtgaagcaaa aggaccaaat gtatgaagat
     7081 aaggtctaaa cccctgaata gacaagcctg ctgcaaggcc gaccatttct gcttccataa
     7141 tcccaacatt cacataacgg tctccaaagt ccttttcaag attattagta gccatcgaac
     7201 ttgacaaatc ggcttctaag actactatat cagaatcact ttgattagcc tctaaaagga
     7261 agtctctata tacatgccgt aattctttcg tacttctcat cattctgttt cctccaattc
     7321 ctgacttaat ctttctacaa ctgaagttaa catttgtttc tcctctacag tagggcgaag
     7381 atgatgattg gatttcattt cttccagctc ttgaacccct tgacctttaa tagtatctaa
     7441 tacaatgcac ttaggtgatg aattatttga ctgttttaat tggacaatcc cttcataaat
     7501 ttctctaata tctgaaccct tgaccctaat ggattcaaat ccaaatgctg aaaatttttc
     7561 tacgaaatca cctggattac aaatatcctt tgtaaaacca tctaattgtt ttttgttatc
     7621 atcaacaaat acaattaagt tggataactg ttgatgagaa gcaaactgta tagcctccca
     7681 acattgtccc tcatttaact caccatctcc aacaatagcg taagtataaa agggactctt
     7741 tcttattctc tgaccatatg caagtccagt tgcaacacta attccttgtc ctaaagagcc
     7801 cgttgtcata tctatgcctg gcgttagatt tctatcagga tgagacggta atttggttcc
     7861 atttgtattt aaagaatata agaattcttt gtcaaagaaa ccattcaaat agagtgtact
     7921 gtatagagct ggtcctccgt gaccttttga taatatgaaa taatctctat ctcgtgctgc
     7981 aaatatttct ggagtcattg gcattatttc accataaagc accgctaaaa cttctacgat
     8041 agacagactt cctccgtaat gtccgaatcc aagatgattc aatgttctaa gagtatttaa
     8101 tcggatgtta gtcgcaaatt ttcttaaccc atcttctcta tttttactta aaatcatccc
     8161 ttattcctcc gttgcagatg gctttttaat aaaggatact ccaaacataa ctgctagaat
     8221 aagaacaaga ccaatcacaa tgcctgcttg tgagccaaat tgatttaaca ttcctaaaat
     8281 aattcctgat agaccaaaat ctgcatctga gaaagttgat ccttggaaac caagtcctcc
     8341 caaaactggc attaaaaaga ctggaagaaa actgattaaa ataccttgta aaaatgctcc
     8401 aatagtggct ccacgaacac caccagatgc attcccaatg acacctgcag tcgctccaca
     8461 gaagaaatga ggcacaacac ctggtaagat aacaaccgtt cctgaagcaa tcataattac
     8521 catacttact aaaccaccaa caaaactaga gataaatcca attagaactg cattgggtgc
     8581 ataagtataa acaatcggac aatccaaagc aggttttgaa ttaggtacaa gacgctctga
     8641 aataccttta aaggctggaa caatttcgcc caaaataagg cgaacacctg ctaaaataac
     8701 aaatacccct gctgcaaatt gacctgctaa ttgtaaagca taaactagac cacttgtacc
     8761 actactgatt tctttttcta tatattctga ccctgcaaag atagctacaa taatgtaaat
     8821 aactgccatg gataaagtaa tactaacagt actatcacgt aaaaaagcta aactctttgg
     8881 aaatttaatg tcctctgttg attttgattt gtcaccgata aggctaccag taaaaccact
     8941 caaccaatat cccaaagaac tgaaatgacc taaagctacc ttgtcatttc cagttaattg
     9001 aaccatatat ttttgcacaa atgctgggga aatactcata ataataccga gtgctaatcc
     9061 tcctagtaag atgagaggca agctagtaaa gccagcaact gataaaatga ccgcaatcat
     9121 acatgccata tatagagtgt ggtgccctgt taaaaaaata tatttaaatc gagtaaaacg
     9181 agcgattaag atattgaaca ccatgcctgc aaacataatc attgcagtag ctgagccata
     9241 tgttgttaaa gctacagcta caattgcttc attattcggc acaacgccag ataaatgaaa
     9301 agcatgctca aacatggtac caaatggatt taaagaattt tgtacaattc ctgcaccacc
     9361 agatacaact aagaaaccaa caaaggtctt aattccacct ttaataatat caggtaattt
     9421 cttcttctga agaactaatc ctaagattgc aattaaagct actaaaatag ctggtgtact
     9481 aacaatatcc aatatgaact tcatcatgac gctagcctcc tatataagtc ctttttcttc
     9541 acaaagttta gtaattaatt ctcgtagttc atccatatca ataatactat ttaagatacg
     9601 aacatctcca agatgactag ctgaatcagc tagatcacga ccaacaatcc aaatatcagc
     9661 tgcatttgga tctgctccac ctaaatcata atgttcaact tctacatccg aaacattcaa
     9721 atcactcaat acagattcaa tattcatctg taccataaaa cttgaaccta atcctgaacc
     9781 acaagctgta ccaattttta acattatcta atcctcctgt ttaattatca ttttaatgtc
     9841 atcatagttt tttgatgata ttaaagtttg aacatgattt ttatctctta aaattgttgt
     9901 taaatgtgac aaagccttta aatgactctc attatcaatg gctgcaatac aaatcaacaa
     9961 tcttacctct tgttctggat tatccaataa ataaatcggt tcttccaaaa ctaacattga
    10021 cattcctatt tcattcacac cttcatctgg ccgagcgtga ggaattgcta ctcccttccc
    10081 taaattaata aaaggtccaa actcttctac tttttgaatc attgcctcag ggtagttctc
    10141 agttatctta tcttgatcca aaagcggttt agctgctaaa cgaatcgcct ccttccatcc
    10201 taatttttgc gaactaacct gataggtttc tttggtaata agttgttcta gcactggtac
    10261 aatttccttt ctatcatttt tttggtaaag ataattcttt aacgccaatc ttaattccaa
    10321 ttcttgtgta ataattccat atcttttgac aatattcagg atttgttcaa tctcaaaatc
    10381 tccatactct aaattcggaa aatcttttaa cactagttct actagttgta ttgcttgctc
    10441 ttcagtcatc ataaccgaaa ctagataatt tggcttttct gtctccacct ttatggtaga
    10501 aaaaaccata tcatagtcac tactagcttt cacctgtaaa tcatcaatct ttgaggttcc
    10561 tataaactca atttgaggaa ataatgctaa tagattctct tttaacatca atgaagaact
    10621 aacaccatta ggacaaatga ttgctgcttt ataccatttt tgaggcaaag tatctgcttt
    10681 ctttaaataa cctccgaaat ggataacaaa atatgctgtt tcactatcag gtatgggatt
    10741 gtcaatagcg tccatcaagg gcatcaaaga atctttgact aattcaaata aatcaggata
    10801 atgttcttta acatgcaata catattcatt tgaactaggt aggccgaact ttaatctata
    10861 gtaagccggt ataaggtggc ggcgaagatt ttctctcaat ccttcccttt gtttaaaatg
    10921 taacaaagaa atatcttcca ttctacttat aatagcctct gttaattgat taaagtaaac
    10981 cggagcaaca tctacttcac cttcaaagca acttgataat aaaacggtga tatagcgata
    11041 atcatcctca gaaaacaccg tatctataat tcccaaatca accactgtat ccaataaaat
    11101 agtggttata tcttgaataa caggagatac taatgtctct gaaagacata ctctttcaac
    11161 atccctttga tacctacaca gaatgaatac taaaccgaaa aggtaaactt ttaattgatt
    11221 aacaataggt actagctgta gcttctcata ataatcttta actacctgat caatcaaatc
    11281 ataagttaat gaataccccc aactggataa aacataatcc aaaccccaaa tccctatgga
    11341 ggattccagc aactcactaa ccatttgaaa agctaagcgg tgcttattcc actctgaacc
    11401 gtgtaaagta taaccttttg ctctactgta ccctagctcc aaatcattat ctaacataat
    11461 ctttcttaat gattgaatat cagataaggt tgtattctta cttactttca aaaagtcttg
    11521 gtaatgacta ttcgatataa aatctaatcg gcaaaaagtg taaagataga ttaaagctaa
    11581 gcgagtcgac tttggtaaaa ccaattcatc cgacttaata atatctgtca aagactgctt
    11641 cgtacgattt gataaactat agcgaccttg ctttttatcc agcactatcc ctttattagc
    11701 tagataaggc actaaataat ctattccttc tttgacttcc tttataggta agctcacctt
    11761 aacagataat tcatataatg atagctcaca atgatccatc aaagtcatca aaataactag
    11821 tgctctataa tcaaacattt ctcttccttt ctaactacaa ttatagtgta aaaattataa
    11881 gaataaaaga gtatttgagc acaatataat ctcacctttt aaaagaaatt attgggctga
    11941 aagtttttag tgattatttt tcaactttct attgacttta taaattaaag tgatatcatt
    12001 tagatatcaa ataacaggag gtcattctta tgactgaaaa actaatcaat tcaaaaccaa
    12061 atggtgtatt cgcattgatt ctcattgagt tgacaatcgt acttggtatc tttatattta
    12121 taatgggggt tggttcggaa aacatttttg gaattattat cggaccttta ctaatcgtaa
    12181 ttgcaggtct agctcatgct ggtttaaaag ttgtcaaacc tcaagaagct ctggttctga
    12241 cactctttgg taactataca ggtaccatca aagaacctgg cttttacttt gtcaatccct
    12301 tcagcgtagc agtcaaccct gcaaaccaca ctcgacttgg acaaagtggt gatgttagca
    12361 caaaatctcc ttttttagga gctaaatcat caaatgacaa tgatgtaaat cttgaaattg
    12421 gcaagaaaca gatttccctc aaagtcatga ccttgagcaa ttctcgtcaa aaaatcaatg
    12481 attgcttagg aaaccctgta gaaatcggta tcgcggtaac ttggagagtt gtcgataccg
    12541 ctaaggcagt cttcaacgtt gataactaca aagaatatct ttcattgcaa tgtgatagcg
    12601 ccctccgtaa tattgtccgc atctatcctt acgatgtgtc tcctaatgtg gatactacgg
    12661 gtgatgggca agcagatgaa ggtagtctcc gtggctctag cgaaattgtt gctaaccgta
    12721 ttcgtgaaga aatccaaagt cgtgttgagg atgctggctt ggaaatcctt gaagcacgta
    12781 tcacttacct agcttatgct ccagaaattg ctgccgttat gcttcaacgc caacaagcat
    12841 ctgccattat tgatgcacgg aagatgattg tagatggtgc tgtaggaatg gttgaaatgg
    12901 cactagaacg gctcaatgaa ggggaattgg tagaacttga cgaagaacga aaagctgcca
    12961 tggtttcaaa tctccttgtc gttctttgtg gcaatcatga tgcacaacca attgtcaaca
    13021 caggaagcct ttactaaaga tggctgaagc taaaaaaaag cagatccccc ttcgactctc
    13081 aacaaagtta tacgctgcac tcgcatcatg ggcagaagac gactttcgtt cagtcaatgg
    13141 gcaaatcgaa tatctcctca cagagtgtgt caagcaacgg aagaaagacg gaaaatacgt
    13201 atcagaaacc attgacgaac catttgagat tgatatttaa caccttctcc tgtctgccgt
    13261 gtcggatagg agaatttttt catctttttt tgtcaagtaa ctttacttta caaaaaaaat
    13321 gtgttatcct agtatggttg atgaaaatca gtagattgaa tcgaatataa atacctaaag
    13381 gagaaatcaa aatggcagta cctgcacgtc gcacttcaaa agcgaagaaa aacaaacgtc
    13441 gtacacacta caaagtaaca gctccatctg taaactttga cgaaactact ggagattact
    13501 cacgttctca ccgtgtatca cttaaaggat actacaaagg acgtaaaatc actaaagctg
    13561 catcagctga ataatagaag ggagatacca tgcgcgtaaa tattacactt gaacacaaag
    13621 aatctggtga acgcttgtac cttacttcta aaaacaaacg taacactcca gaccgccttc
    13681 aattgaagaa atactcacca aaacttcgca aacacgttgt gtttacagag gttaaataag
    13741 cattcaatac gaatcaatac agaagaaaaa cgcttgatca gcgttttttc tttttgttaa
    13801 tttcactaca ttgtaaggga aatcattcga atgactacat ttttcactac cttttttgaa
    13861 ataagaatac ggattcggac aattaaaagc aagaggaact caaatgtaag ttggggttcc
    13921 ttttttgtat atttttcaaa taacgcctct acactatttg tagcaaattc accaactaca
    13981 gttgtatctt agttaaaata agttagaata tgtaagtgag taccagatat accaagacat
    14041 cgtcaccatc taaggtatat tcaaaataca aaagttgacc aactagattt ctgaatatcc
    14101 ttatatatcc attcttaaaa ttggtttaaa tagcgtagtc ttttaaacta gttttgagaa
    14161 tccaaaaaat cttcctacat atgtaagaag attttttagt tcagaatgat tagatttagc
    14221 taatggatac ctatcctacc cactcaccgt tagcattgac tcgatagcca tcgggtgtag
    14281 tcgtgtttac taataaatct cctgaattat aggtatagta ccatttacca gaaactgtaa
    14341 cccaaccagt agccattgaa cctgattcat ttaagta
//
LOCUS       DAVBCN010000052        14357 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVBCN010000052 DAVBCN010000000
VERSION     DAVBCN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14357
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.052"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            65..721
                     /locus_tag="VQ497_001458"
     CDS             65..721
                     /locus_tag="VQ497_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HET2517755.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            711..1034
                     /locus_tag="VQ497_001459"
     CDS             711..1034
                     /locus_tag="VQ497_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HET2517756.1"
                     /translation="MVSKYLLLAVIFSDLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAV
                     LRLVF"
     gene            1138..1968
                     /locus_tag="VQ497_001460"
     CDS             1138..1968
                     /locus_tag="VQ497_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2517757.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NSSPKPFNYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYQMAV
                     NNISYTKERAEKYLYAAPTVKDPNVLVVKKDDSSIKSLDDVGGKSTEVVQGTTSAKQL
                     EDYNKQHSDNPTILNYTKANLQQIMGHLSDGQFDYKISDKITVETVIKNQGLDNLKVI
                     DLPSDQQPYVYPLLAKGQDELKTFVDKRIQELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2009..2086
                     /locus_tag="VQ497_001461"
                     /pseudo
     CDS             2009..2086
                     /locus_tag="VQ497_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC protein"
     gene            2189..3019
                     /locus_tag="VQ497_001462"
     CDS             2189..3019
                     /locus_tag="VQ497_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2517758.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NGSPKPFIYEENGELTGYEIEVVRAIFKDSDKYNVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            3173..4027
                     /locus_tag="VQ497_001463"
     CDS             3173..4027
                     /locus_tag="VQ497_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HET2517759.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            4127..5500
                     /locus_tag="VQ497_001464"
     CDS             4127..5500
                     /locus_tag="VQ497_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET2517760.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            5493..6554
                     /locus_tag="VQ497_001465"
     CDS             5493..6554
                     /locus_tag="VQ497_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HET2517761.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            6556..7248
                     /locus_tag="VQ497_001466"
     CDS             6556..7248
                     /locus_tag="VQ497_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2517762.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(7278..7847)
                     /locus_tag="VQ497_001467"
     CDS             complement(7278..7847)
                     /locus_tag="VQ497_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HET2517763.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            7982..8596
                     /locus_tag="VQ497_001468"
     CDS             7982..8596
                     /locus_tag="VQ497_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET2517764.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVVLIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            8598..10244
                     /locus_tag="VQ497_001469"
     CDS             8598..10244
                     /locus_tag="VQ497_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2517765.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYYRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDR
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQKYFEHRASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            10256..11542
                     /locus_tag="VQ497_001470"
     CDS             10256..11542
                     /locus_tag="VQ497_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2517766.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGCD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            11603..12136
                     /locus_tag="VQ497_001471"
     CDS             11603..12136
                     /locus_tag="VQ497_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2517767.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRTAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKASAEEEETEELA"
     gene            12214..12684
                     /gene="nrdI"
                     /locus_tag="VQ497_001472"
     CDS             12214..12684
                     /gene="nrdI"
                     /locus_tag="VQ497_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HET2517768.1"
                     /translation="MKIISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(12977..14239)
                     /locus_tag="VQ497_001473"
     CDS             complement(12977..14239)
                     /locus_tag="VQ497_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HET2517769.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFSGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
BASE COUNT         4254 a         2678 c         3264 g         4161 t
ORIGIN      
        1 ttattaagac aatatgagga gaaagatgaa agaaaaagga ttttaggagg gggcgcaggc
       61 agccatgcca acggctcttg gctatgtcag tatcggcctg gcctgtggga ctatcggtgc
      121 gccctatgtg acacctgttg agatgggctt gatgagtctc tttgtttatg ctgggagtgc
      181 ccagtttgcc atgttggcac tgattgtggt tcaagctcct gtggcagcta ttgctatgac
      241 ggtttttcta atcaacttgc gtctcttttt gttgagttta cacgcatcga cttatttccg
      301 tcataccagt ctttggtaca atatcggtat gtctagtatc ttgacagatg agacctatgg
      361 cgttttgatg ggtgaattgg cccatacaga caaggtaaat cctatgtgga tgcacggaaa
      421 caatcttaac agctatgtgg cttggtttgt ggggacagta gtcggaacgg ctctgggtgg
      481 cctgctacca aatccagaaa tctttggctt ggattttgcc ctggttggga tgtttattgg
      541 tatttttgct tcgcaatttc agattatgca aagacggatt cctgtccgca atctgctcat
      601 tatcctagca gttgttgcgg tgtccttctt tttgctcttg acagtggtgt ctcagtcact
      661 agctgttctg tttgcgacgc tacttggttg tagcatgggg gtggttttag atggtcagta
      721 agtatctttt attagcagtt attttctctg acttggtgac ttggattctc cgtatgattc
      781 ccttcatctt agtcaagtat aagggcttgc ctgcaatcgt tgagcgtttt ttgaagttct
      841 tgcccgtttc cattatcttt gccttgattc tttcaagcgt agtgacaggt aaggttggga
      901 gccttcctca aattaaatgg ctagacttct tagccgtctt tccaacagct tgggtagcct
      961 ttcgctaccg caatctagtc ggaacagttc tctttggagt agtcttgatt gccgtcttgc
     1021 gtttggtctt ttaaatcagc cataaagaaa acctatcaca gatatatata tcatataatg
     1081 gtgtaaatga tttttttctg ttaagattat aaggtattct attttggagg aaatgacatg
     1141 aaaaaaatcg ttaaatactc atctcttgct gccctagggc ttgttgctgc aggtgtgcta
     1201 gcagcttgct ccggtggtgc taagaaagaa ggacaagcag ctagcaagaa agaaattatt
     1261 gttgcaacta attcttcacc aaaaccattc aactatgaag aaaatggcga attgactggt
     1321 tacgagattg aagtcgttcg cgctatcttt aaagactctg ataagtatga tgtcaagttt
     1381 gagaagacag agtggtcagg tgtctttgcg ggtcttgacg ctgaccgtta ccaaatggct
     1441 gttaacaaca tcagctacac taaagaacgt gcggagaaat acctttatgc agccccaact
     1501 gttaaagacc ctaacgttct tgtagtcaag aaagacgatt ctagcatcaa atcgcttgat
     1561 gatgttggtg gaaaatcaac agaagttgtt caaggaacaa catctgctaa acagttagaa
     1621 gactacaaca aacaacactc agataatcca actatcctta actatactaa agcaaactta
     1681 caacaaatca tgggccactt gagcgatggt cagtttgact acaagatttc tgataaaatc
     1741 actgttgaaa cagtcatcaa gaaccaaggt ttggacaact tgaaagtcat cgatcttcca
     1801 agcgaccaac aaccttacgt ttacccactt cttgctaaag gtcaagatga gttgaaaaca
     1861 tttgtagaca aacgtatcca agaactctac aaagacggaa ctcttgaaaa attgtctaaa
     1921 caattcttcg gagacactta cctaccggca gaagctgata ttaaataatt tcttgaaatc
     1981 atccattctg agtaagatgg gtgatttttc agttcgctac cgcaatctag tcggaacagt
     2041 tctctttgga gtggtcttga ttgccatctt gcgtttggtc ttttaaatta cccataaaaa
     2101 aacttatcac agagatagat atcatataat ggcgtaaatg ctctttttct gttaagatta
     2161 taaggtattc tattttggag gaaatgacat gaaaaaaatc gttaaatact catctcttgc
     2221 agcccttgct cttgttgctg caggtgtgct tgcggcttgc tcaggtggtg ctaagaaaga
     2281 aggacaagca gctagcaaga aagaaatcat cgttgcaacc aatggatcac caaagccatt
     2341 tatctatgaa gaaaatggcg aattgactgg ttatgagatt gaagtcgttc gcgctatctt
     2401 taaagattct gacaaatata atgtcaagtt tgaaaagaca gaatggtcag gtgtctttgc
     2461 tggtcttgat gctgaccgtt acaatatggc tgtcaacaat cttagctaca ctaaagaacg
     2521 tgcggagaaa tacctctatg ccgcaccaat tgcccaaaat cctaatgtcc ttgtagtgaa
     2581 gaaagatgac tctagtatca agtctctcga tgatatcggt ggaaaatcga cggaagtcgt
     2641 tcaagccact acatcagcta agcagttaga agcatacaac gctgaacaca cggacaaccc
     2701 aactatcctt aactatacta aggcagattt ccaacaaatc atggtacgtt tgagcgatgg
     2761 acaatttgac tacaagattt ttgataaaat cggtgttgaa acagtgatca agaaccaagg
     2821 tttggacaac ttgaaagtta tcgaacttcc aagcgaccaa caaccgtacg tttacccact
     2881 tcttgctcag ggtcaagatg agttgaaatc atttgtagac aaacgcatta aagaactcta
     2941 caaagacgga acgcttgaaa aattgtctaa acaattcttc ggagacactt atctaccggc
     3001 agaagctgat attaaataat ttcttgaaat catccattct gagtaagatg ggtggtttct
     3061 cagtttttag ggatatagtt ttaaactata tatctgacta tctaataaca atttgtgaaa
     3121 tcaaacaaat tgtgagatac tagtacggta ttatttttaa ggagaaagaa tcatgaaaat
     3181 caaaaaatgg cttggcctag cagcccttgc tacagtcgca ggtttggctc ttgcagcttg
     3241 cggaaactca gaaaagaaag cagacaatgc aacaacgatc aaaatcgcaa ctgttaaccg
     3301 tagcggttct gaagaaaaac gttgggacaa aatccaagaa ttggttaaaa aagacggaat
     3361 taccttggaa tttacagagt tcacagacta ctcacaacca aacaaagcaa ctgctgatgg
     3421 cgaagtagat ttgaacgctt tccaacacta taacttcttg aacaactgga acaaagaaaa
     3481 cggaaaagac cttgtagcga ttgcagatac ttacatctct ccaatccgcc tttactcagg
     3541 tttgaatgga agtgccaaca agtacactaa agtagaagac atcccagcaa acggagaaat
     3601 cgctgtaccg aatgacgcta caaacgaaag ccgtgcgctt tacttgcttc aatcagctgg
     3661 cttgattaaa ttggatgttt ctggaactgc tcttgcaaca gttgccaaca tcaaagaaaa
     3721 tccaaagaac ttgaaaatca ctgaattgga cgctagccaa acagctcgtt cattgtcatc
     3781 agttgacgct gccgttgtaa acaatacctt cgttacagaa gcaaaattgg actacaagaa
     3841 agcacttttc aaagaacaag ctgatgaaaa ctcaaaacaa tggtacaaca tcattgttgc
     3901 gaaaaaagat tgggaaacat cacctaaggc tgatgctatc aagaaagtaa tcgcagctta
     3961 ccacacagat gacgtgaaaa aagttatcga agaatcatca gatggtttgg atcaaccagt
     4021 ttggtaataa gaaacaggga ggtgggagag aaaattccac ctcttgcttt tgtatagagt
     4081 atagattgta aagaagacta ttcgttcata gaaaggtaga gagaatatgg tttttcctag
     4141 cgaacaagaa cagattgaaa aatttgaaaa ggatcatgta gtccaacatt attttgaggt
     4201 tttgcgtacc ttgatttcta agaaatcagt ctttgcccag caggttggac tcaaggaagt
     4261 cgcaaattat ctgggtgaga ttttcaagcg tgttggagct gaagtggaga ttgatgagag
     4321 ctatacagcg ccctttgtca tggcacattt caagagttcg cgtccagatg ccaagacctt
     4381 gattttctat aaccactatg acactgtgcc agcggatggg gatcaggtct ggacagagga
     4441 tccttttacg ctttcggtcc gcaatggctt catgtatggg cgtggggttg atgacgacaa
     4501 gggtcatatc acagctcgct tgagtgcttt gagaaaatat atgcagcacc atgatgattt
     4561 acctgtcaat atcagcttta tcatggaggg agcggaggaa tcggcttcaa cagacctaga
     4621 taagtatttg gaaaagcatg cagacaaact ccgtggggcg gatttgttgg tctgggaaca
     4681 agggaccaaa aatgccttgg aacagctgga aatttctggt ggcaataagg ggattgtgac
     4741 ctttgatgcc aaggtaaaaa gcgctgatgt ggatatccac tcgagttatg gtggtgttgt
     4801 ggaatcagct ccttggtatc tcctccaagc cttacagtct cttcgtgctg cggatggccg
     4861 tatcttggtt gaaggcttgt acgaagaagt acaagagccc aatgaacgag aaatggcctt
     4921 gctagaaact tatggtcaac gaaacccaga ggaagttagt cggatttatg gattggagtt
     4981 gcctctctta caggaggagc ggatggcctt tctaaaacgt ttctttttcg agccagcgct
     5041 taatatcgaa ggaatccagt ctggttatca aggtcagggt gttaagacta ttttacctgc
     5101 agaagccagt gccaagctag aggttcgtct ggttccgggc ctagaaccgc atgatgttct
     5161 ggaaaaaatt cggaaacaac tagacaaaaa tggctttgat aaggtagaat tatactatac
     5221 cttgggagag atgagctatc gaagcgatat gagcgcacca gccattctca atgtgatcga
     5281 gttggccaag aaattctatc cacagggcgt ttcagtcttg ccgacgacag cggggacagg
     5341 acctatgcat acggtctttg atgccctaga ggtaccaatg gttgcatttg gtctaggaaa
     5401 tgccaatagc cgagaccacg gtggagatga aaatgtgcga atcgctgatt attacaccca
     5461 tatcgaatta gtagaggagc tgattagaag ctatgagtag agatattatc aagttagatc
     5521 agatcgatgt gacttttcac caaaagaaga gaaccatcac agcggttaag gatgtgacca
     5581 ttcacatcca agaaggggat atctacggaa tcgttggata ttctggagca gggaaatcaa
     5641 cccttgtacg ggtgattaac ctcttgcaaa aaccatctgc agggaaaatt accattgacg
     5701 acgatgtgat ttttgatggt aaggtcacct tgacggcaga gcagttgcgt cgtaaacgtc
     5761 aagatatcgg gatgattttc cagcatttta acctgatgag ccaaaagaca gcagaggaga
     5821 atgtagcctt tgcccttaaa cactctggac tcagcaagga agaaaagaag gctaaagtag
     5881 ctaagttgtt ggacttggtt ggtttggcag atcgtgctga aaactaccct tcacaactat
     5941 ctggagggca aaaacagcgt gtggcaattg cgcgtgcctt ggccaatgat ccaaaaatct
     6001 tgatttcaga cgagtcaact tctgcccttg atccgaagac aaccaagcag attttggcct
     6061 tgttgcaaga tttgaaccaa aaattaggct tgactgttgt cttgattacg catgaaatgc
     6121 agattgtcaa agacattgcc aaccgtgttg cagttatgca ggatgggcat ttgattgaag
     6181 agggtagtgt gcttgaaatc ttctcaaatc ctaaacaacc tttgactcaa gactttatct
     6241 caacagctac aggtattgac gaagccatgg tcaaaatcga gaagcaagaa atcgtggaac
     6301 acttgtctga aaacagtctc ttggtgcaac tcaagtacgc tggagcttca acagacgagc
     6361 cacttttgaa tgaattgtac aagcattacc aagtaatggc taatattctc tatgggaata
     6421 tcgaaattct cgatggtact cctgttggag aattggtggt ggtcttgtca ggtgaaaaag
     6481 cagcgttggc aggtgcccaa gaagccattc gtcaagcagg tgtacaacta aaagtattga
     6541 agggaggaca gtaagatgga atcattgatt caaacctatt taccaaatgt ctataaaatg
     6601 ggttgggctg gtcaggcagg ctggggaacg gctatctact taactcttta tatgacagtt
     6661 ctttccttca ttatcggagg cttcttgggg ctagtggcag gtctctttct cgtcttgaca
     6721 gcgccaggtg gtgtcttgga gaataaagtc gtattctgga ttttagacaa aattacctca
     6781 atttttcgtg cagttccctt tatcatcctc ttggcaatct tgtcaccact ttctcacttg
     6841 attgttaaaa caagtatcgg gccaaatgca gcccttgtcc cactttcttt tgcagtcttt
     6901 gccttctttg cccgtcaggt gcaggttgtc ttggctgaat tggatggcgg tgtcattgag
     6961 gcggctcaag cgagcggagc gactttctgg gacatcgtgg gtgtttacct atcagaaggt
     7021 cttccagatt tgatccgtgt gacgactgtg accttgattt cccttgttgg ggaaacagct
     7081 atggccggtg cggttggagc tggtggtatc ggtaacgtag ccatcgctta tggatttaac
     7141 cgctacaatc acgatgtgac catcttggca accatcatta tcattttgat tatctttgca
     7201 atccaattct tgggagattt cttgactaag aaattgagcc ataaataaaa aagagccgcg
     7261 tggctctttt taactgatca gattttctgt gcaaattttt tactcaaggc ttgtccaatc
     7321 aaggcaccca ctagggctcc gatgacaata cttgcgataa atagaaggac agttccaggg
     7381 tttggagcga ccatgatgcg gtcgatatat tcttgggatt ttcctcttgc cagaagagta
     7441 gccatatagg ctttgggcgc aatccacata agcaagattg gtcctgttgt actaaaggcg
     7501 aaaataatga aagaaaggaa gttctttgtt ttgtccttgt attttcctaa atgagctact
     7561 ccatctgcta ggaggccaca gataattcca ggaaggaagg aaccagcacc gtgtttagtt
     7621 cccaagaaaa agagggcaat gacaaggccg atagtggtaa tggctccaaa gcgcggaact
     7681 tttgcgacta ggatcatata gacgctaccg ccgacaaggg cagtaaaggc aggcgcataa
     7741 aacatgtttc cagtttggtc aaagagattg cccaaaagga caccaatccc catgcagagg
     7801 aagtaaagaa ctgcaaaaag cagtgtagtt aagatagatt tcttcatgaa ttgtctcctg
     7861 ataatttttt cacaattctc attttaccat ggtttggtgg gattgtaaat ggatactaga
     7921 agtttttgaa aacgcttgaa atatgatata atggtttcat agttattttt aggggaatat
     7981 catgggtaga tttttagact ttgtctttaa tcgtttcttt ttagggatga ttgcgacagc
     8041 cttcttttgg ctattaactt tagcaggagg gattatcctt ggtctagcgc cggctagtgc
     8101 caccttgatg agcttatatg cagaacatgg ttatagcttt cgggaataca gtttgaagga
     8161 ggcttggtct ctttacaagc aaaattttgt ctcaagcaac ctgattttct atagcttttt
     8221 aggtgtgggt ctagttttga cctatggttt gtatctcttg gtgcaattgc ctcatcagac
     8281 tattgttcat ttgattgcga cccttttgaa tgtcctagta gttgtcctga tctttttggc
     8341 ttatacagta tctttaaaat tacaagttta ttttgccttg tcctatcgaa atagtctcaa
     8401 attatccttg attggcatct ttatgagtct agcagctgtg gccaaggttc tccttgggac
     8461 tgtgctactt gtagcaattg gttactatat gcctgccctg ctattttttg taggaattgg
     8521 gatgtggcat ttctttatca gtgatatgtt ggaacctgtc tatgaaatca tccatgaaaa
     8581 attggcgaca aaatagaatg aagcagtttt ggctacatac gcttctaaga acctatagtt
     8641 cagtgatgat cattatcatt gcgagttttg caatcttact ctcttacgct gactgggatt
     8701 cacgtgaaaa ggaagcccag agagtagccc agcgtgtaac tgctagaaca gtgagtgaaa
     8761 ttgaatatta ctatagagag tcaacccaga tagctcaggc tttagttgaa aatcaagctc
     8821 gtattgaggg aatctataaa tactttagcc ttagcatgcc agactatttt tactggcaat
     8881 tagagcggaa agcttcgcct tatatatcag tctctctgta tgaaaatgtt gatgacctct
     8941 atgttcgaaa tgattttgta actggggtgg ccattgcttt tcaagattac aaggaagtct
     9001 atgtttctac taaagacaaa cgtagtggag aaaaaatcag ggctgaggat ttcaaaccag
     9061 caggaaatag ttttgccatt ccggtgtcag atccagtgtc agatcaagat ttaggagtga
     9121 tttacatctc cttggatcct gctgttttat accatgccat tgataatact agaggtcata
     9181 ctccgatggc agtaacagtg acctcacctt ttgatacgga gatttttcat attggtgaga
     9241 cagttgatag ggagagtgaa aattggctag ttggcttaac ttctcatgga tatcaggttc
     9301 aggtggcagt tcctaaaaac tttgttttac aaggaacagt gactagctct gctttgattg
     9361 tgggtttgag ccttctcttt attgtcattc tttatctgac tttgaggcag acttttgcta
     9421 attaccaaaa gcaggtagtg gatttagtgg aatccattca agtcattgct caaggcgaag
     9481 aggggcgtcg gattgacatt tccgaaaaag atcaggaatt actcctaatc gcggagacga
     9541 ccaatgatat gttggatcga ttggaaaaga atatccatga tatttaccag ttagagctta
     9601 gtcaaaaaga tgccaatatg cgagccttgc aggcgcaaat caatcctcat tttatgtata
     9661 atacgctgga gttcttgcgc atgtatgcag ttatgcagag tcaagatgag ttggcagata
     9721 tcatttatga attcagtagt ctcttgcgta acaatatttc cgacgaaaga gagaccctcc
     9781 tcaaacagga attagaattt tgccgtaaat acagctatct ctgcatggtt cgctatccca
     9841 agtccattgc ctatggtttc aagatagatc cagagttaga gaatatgaag attcccaagt
     9901 ttaccttgca accgctggta gaaaactatt tcgcgcatgg tgttgaccac aggcggacag
     9961 ataatgtgat tagcatcaag gctcttaaac aggatggttt tgtggaaatt ttggtggtcg
    10021 ataatggtag aggaatgtcg gctgaaaagt tggcaaatat ccgagaaaaa ttaagtcaga
    10081 aatattttga acaccgagcc agctacagtg atcaaaggca gtctatcggg attgtcaatg
    10141 tacacgagcg ttttgtgctc tattttggag accgctatgc cattacgata gagtctgcag
    10201 agcaagccgg tgttcagtat cgtattacaa ttcaagatga gtagaaaggg agaaaatgta
    10261 taaagtatta ttagtagatg atgagtacat ggtgacagaa ggtctgaagc gtttgattcc
    10321 ctttgataag tgggatatgg aggtcgtcgc aacagccagt catgccgatg aagctctaga
    10381 atatgttcag gaaaatcctg tcgatgtcat catttccgat gtcaatatgc cagacaaaac
    10441 agggcttgat atgattcggg agatgaaaga gatcttacca gatgctgcct atatcctgct
    10501 ctcaggttat caggagtttg attatgtaaa aagagcaatg aaccttagtg tggtggacta
    10561 tttggtcaag cctgttgata aggtagagct gggaaatctg ctggagaaga ttgcaggtca
    10621 gctcggcgag agagggaaga aaagtcagac tcttagtcaa gaattagacg aggctggatt
    10681 tgttagttat ttaggggata aggagaattg gtggataggt ctatccaagg aaaaacaagg
    10741 ttccttcacc attccctact atgtcttggg ccaagactgg cagattttca tttctgacca
    10801 acccctagat ggtttagtcg ttacaccttt tgaagctcct tatcaagaac actttgaacg
    10861 ctggaagctg aatgctgaga aaaccctctt ttacggttct gtaaatttgc agcagtctga
    10921 gagtctcttt gcctattacg aaccgattta tagggttatc attcagggaa atctcaatca
    10981 aatcgtagaa gagttaaatc tcttggagaa ggtagttctt gaaaatacgc cgcgtgttcc
    11041 gattactaaa cagcttttta ttcagtttgt catggatgtt ttccatttat ttgaacatct
    11101 caaagctgat gacctgacag acatcgtcaa aaccattcat gctattcaat ccttcgatga
    11161 attggttcct tatatcaagg aaactctgag cagctttttc ggtcaatacc gtatgaatga
    11221 aaatgtggtc agtgtgctgg aagtcattgg ttgtgattac caaaaagagc tttccctcaa
    11281 ggatatcagt aaggctctct ttatcaatcc tgtctatcta gggcagttga ttaagcgtga
    11341 aaccgattcg acttttgcag agttactaaa caaacaacgt attaaggctg cccaacaact
    11401 tttgctttca actagtgaca gcatcgaaga tatttgttat gctgttggtt acagtaacct
    11461 tggatatttc tataaagttt tccgaaaatt gtgcggaaaa tcgccaaaag cctaccgaaa
    11521 acaggtagaa actatactat aagatttgta ttcctttaca aaatgtgcta taatataaat
    11581 attattatta ggaggttcta tcatgaaaaa gaaaccaatt tatctatggg tcttgctaat
    11641 cttgtctgcc cttatttcag ttccgtctct gtttggaatt gtgagtccct tgcctagtaa
    11701 agaagccctt cgtactgctc agaaacaagt tgcaggggtc aatgctcagc aattagaaga
    11761 ccagcttaat tatacctata gagtagcaga agcatctcat tctattttta atgttgcttt
    11821 gattgtgcta tctactattt tagtggtcgt agcgattgtc ttccttgttc gtaaaaattt
    11881 gcaatatgca aactatactt acgttggcta tgttttgtta gctattattg gttcgattta
    11941 tggctatgtt ggtttacaag acgctgtgca gttggttcaa gatgagagca tgcgtttgac
    12001 agtgagtatt ggttcaaaag ctgttagcat tttctatatc gtcatcaatg ttctcttcct
    12061 agcccttgtc ttctataaga tgtggcgaca acagaaagcc tcggcagaag aagaggaaac
    12121 agaagagctt gcctaagtat tgacaaaaaa gaactatcaa gatacaatct tggtagttgt
    12181 tttttgatta tagataaaat catggaggta catatgaaga taatttcttt agtttatatt
    12241 agtttgagtg gcaacactga aagttttgtg acgcgcttga aagactatct cttgtcccag
    12301 tacaaaggga ttgaggttca aaagattcat atcaaggatt tagtcaagga aggcaaaaat
    12361 ttctatgaaa tggaccatcc ctatgtcgct tttttgccga cctatctcga aggtgggaat
    12421 ggcgtggata acggagatgt tgagattttg acgacaccgg tgggagattt tattgcctat
    12481 ggtaacaatg ctagtaagtg ttttggtgtg gttgggtcag gaaatcgtaa ctttaataac
    12541 caatactgcc taacagccaa gcaatacagt caacgctttg gctttcctgt attggctgac
    12601 tttgaaatgc gaggcatgct ggaagatatc aaacatgtcg cagctattat cgcagatttg
    12661 tatgagttgg aaaaggagaa ttaatactca atgaaaatca aagagcaaac taggaagcta
    12721 gtcgcaggtt gctcaaagca cagctttgag gttgcagata gagctgacgt ggtttgaaga
    12781 gattctcgaa tagtataaaa ttcttataag aggtggttga agttgttcaa ctgttttttg
    12841 agtataaaca gtctttaaga acgtaaaaaa gcataagttc aggtcttcaa aaccttgttc
    12901 ttatgctttt tatcatagaa atatttgctg aattttctct tgaaatgaaa ccttatcttc
    12961 atctcgcgct tgtcccttac cctgcttttc cgattgcgag tgcgtagata aagaagatgg
    13021 cgaagccaaa gaggaaaatc aatcccgcaa tggcaaggtg tttgagccaa gaaggaagat
    13081 ttttgttttc acgcgttacc aaggcgtaat tcaaaagagc aaagaaaggt gttgtcagga
    13141 aagagccaat catggcaaag cggagcatgg ttgaaacctg accagagaag aacttgataa
    13201 tgacgatacc gatgatagca gtgatggtca tccagatgtt caaagattta cgattgtcct
    13261 ctttttgact gattagcagt cggagagatt cctgattaac gcgagaatag ccatcgataa
    13321 cagttataac tgttccaaag atacagagga aggcaataaa ggtaatcaag taacgggacc
    13381 attcgccaag aacagaggca tacatgccca cgaattgaga gatgtatttg gctgaagcag
    13441 cttcaaccgc ctgccctgta ggatactgaa tcagtgctcc cagtgccaca aagaagacgg
    13501 ctaggatagc tgttccaata taaccagtgt taaagtcaaa cagagcgtct tctgtgttaa
    13561 agttgacggt ctttctcttt tcagctgacc aaagtgaatt gatggctgaa atttcaatag
    13621 gagccggcat ccatcctagg agggagacga tgaagggcag agctgccatt tgccaaggtg
    13681 tcttctcgac aaaatcagaa ctgtattctg gatgcttgac cgccgcaatg ataactgcca
    13741 gaacagtcgc aatggttaag gcagacataa tccatttgac catgccgtct aaaagtttgt
    13801 agcctccaaa gagtagcata gcccaaatga ttgcaacgag aatgagggac cactgagtaa
    13861 tgctaagtcc aatcattggg aaggcactgg cgatgatagc tgagcacaga atggcaacac
    13921 cagccgtgtt gaccatagcc gaaaagacat tgaggataaa gaaaatccag agatagagtt
    13981 ttcctttttc ggcataacct tcaaccaaag tctttccagt atcagctgtg tattcagcac
    14041 caaaacggaa aaatggatat ttaaagacat tggctaagat gaccaagaga agtagagacc
    14101 aaccgtaaga accgccagct tgagttgagg atacaatgtg ggaacctcca acagcggcag
    14161 ttgccattag gattccaggt cccattgctt ttagcttgct tgcccaggtt gattgattta
    14221 aggaaatagc ttgcgacatg ataaatactc ctttttgaat tttcagaata atctgtatat
    14281 gtaatctata ttctacaaaa aattttggaa aaatgcaaga atattttgaa aaaagataga
    14341 aaatttacaa agaaaat
//
LOCUS       DAVBCN010000053        14159 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVBCN010000053 DAVBCN010000000
VERSION     DAVBCN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14159
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.053"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..170
                     /locus_tag="VQ497_001474"
     CDS             <1..170
                     /locus_tag="VQ497_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2517770.1"
                     /translation="KACNHALSKERSKVENIFAKVKTFKMISTTYRNHRKRFGLRMNL
                     IVGIINHELGF"
     gene            321..797
                     /locus_tag="VQ497_001475"
     CDS             321..797
                     /locus_tag="VQ497_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2517771.1"
                     /translation="MKLSLYSEVEHYHFLSSYSLNDITYTDTPKNIIEKSKKDKYRYP
                     IVILNKDHKVIGFFCLHLNTGPKEYGYYQNDYALIRGFSIHDNFRNQGYGSNALNEIF
                     EFIDSTLKLEINHIILAVNEKNILAQKAYRNSGFSVVKKDLEGKRGKLIIMEKSRN"
     gene            complement(1471..1863)
                     /locus_tag="VQ497_001476"
     CDS             complement(1471..1863)
                     /locus_tag="VQ497_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET2517772.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLTRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTDKSQYEFLFPSVK
                     IKEEKTDKKEEVATDSSEKAEKKIRREA"
     gene            complement(1841..2302)
                     /locus_tag="VQ497_001477"
     CDS             complement(1841..2302)
                     /locus_tag="VQ497_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET2517773.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVGQLEAELDRSQFEKVEGNRLYMKQDDKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            complement(2265..2525)
                     /locus_tag="VQ497_001478"
     CDS             complement(2265..2525)
                     /locus_tag="VQ497_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517774.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            complement(2530..2958)
                     /locus_tag="VQ497_001479"
     CDS             complement(2530..2958)
                     /locus_tag="VQ497_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET2517775.1"
                     /translation="MEGQIVKSMIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVE
                     EQIFFMEFEELYRETQKRSVASQQKTSLNLDGQMISNGSQKLTVPKGIQAPSGQSITF
                     DRAGGNSSLAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            complement(2927..3253)
                     /locus_tag="VQ497_001480"
     CDS             complement(2927..3253)
                     /locus_tag="VQ497_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET2517776.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLDKNEDATLSKLQGDGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            complement(3255..4271)
                     /locus_tag="VQ497_001481"
     CDS             complement(3255..4271)
                     /locus_tag="VQ497_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET2517777.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLDFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            complement(4219..5160)
                     /locus_tag="VQ497_001482"
     CDS             complement(4219..5160)
                     /locus_tag="VQ497_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HET2517778.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            complement(5236..5601)
                     /locus_tag="VQ497_001483"
     CDS             complement(5236..5601)
                     /locus_tag="VQ497_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HET2517779.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            complement(5752..6810)
                     /locus_tag="VQ497_001484"
     CDS             complement(5752..6810)
                     /locus_tag="VQ497_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET2517780.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPKKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            complement(6973..8124)
                     /gene="nagA"
                     /locus_tag="VQ497_001485"
     CDS             complement(6973..8124)
                     /gene="nagA"
                     /locus_tag="VQ497_001485"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HET2517781.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            complement(8277..10094)
                     /locus_tag="VQ497_001486"
     CDS             complement(8277..10094)
                     /locus_tag="VQ497_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HET2517782.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMIFLLSATAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            complement(10195..11337)
                     /gene="tgt"
                     /locus_tag="VQ497_001487"
     CDS             complement(10195..11337)
                     /gene="tgt"
                     /locus_tag="VQ497_001487"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET2517783.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMLLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFRKYFVEKYGYNKSGRNF"
     gene            11467..12324
                     /locus_tag="VQ497_001488"
     CDS             11467..12324
                     /locus_tag="VQ497_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HET2517784.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            complement(12352..12996)
                     /gene="pcp"
                     /locus_tag="VQ497_001489"
     CDS             complement(12352..12996)
                     /gene="pcp"
                     /locus_tag="VQ497_001489"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET2517785.1"
                     /translation="MKVLVTGFEPFGGEKGNPTLEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSVTLERVAINQDDARISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKFPYVK
                     AGFMHIPYMMEQVVNRPTTPTMSLVDIRRGIEAAIGAMIEHGDQELKLVGGETH"
     gene            complement(13103..13471)
                     /locus_tag="VQ497_001490"
     CDS             complement(13103..13471)
                     /locus_tag="VQ497_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HET2517786.1"
                     /translation="MSIITIILTTIVALEHFYIFYLESIATRSDATSRVFNMEKEELA
                     HPSVSSLFKNQGIYKALLGVFLWYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKYT"
     gene            complement(13482..13907)
                     /locus_tag="VQ497_001491"
     CDS             complement(13482..13907)
                     /locus_tag="VQ497_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET2517787.1"
                     /translation="MIEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIKVKEEMESILTEFERTELSRLLNKLVLGIENIEI"
BASE COUNT         4232 a         3154 c         2480 g         4293 t
ORIGIN      
        1 ataaagcctg taaccatgcg ctatccaagg agagaagcaa ggttgagaac atctttgcca
       61 aagtaaaaac gtttaaaatg atttcaacaa cctatcgaaa tcatcgtaaa cgcttcggat
      121 tacgaatgaa tttgattgtt ggtattatca atcatgaact aggattctag ttttgcagga
      181 agtctaataa tgaaaataaa ggactaaagc aataactcta aatacatctg atttttatag
      241 gatatttcat aaaaaaaatc tacataccta gagtcctatt tagttatgta tcttacataa
      301 agagaaagga attaattatc atgaaattat ctttgtatag tgaagttgaa cattaccatt
      361 ttttaagttc ttattcttta aatgatatta catatactga taccccaaaa aatattattg
      421 aaaagtctaa aaaagataag tatagatacc caatagtaat acttaataaa gaccataaag
      481 taattggatt tttttgccta cacctaaaca ctggtcctaa agagtatggc tattatcaaa
      541 atgattatgc gcttattaga ggattttcta ttcatgataa tttcagaaat caaggatatg
      601 gttctaatgc tctgaatgaa atatttgaat tcatagactc aactttaaaa ttagaaatta
      661 accatattat attggctgta aatgaaaaaa atatattggc tcaaaaagca tatagaaatt
      721 ccggtttttc tgttgtgaaa aaagatttag aaggaaaaag ggggaaatta attattatgg
      781 agaaaagcag aaactagtaa tgatactagt ttctgctttt ctctaagtct gctataaatt
      841 tataacatgt tgaaataaga tgtgaataaa ttgattgcag aattcaaacc aatttctaac
      901 aatgttttag aaaactcggc ggactattcc agattcaatc cactatattc tatcttattt
      961 gcatattttg agagaaataa tctatatact aaacttttat gtttataatt cattcctttc
     1021 gtttcactca aggcacaaga cagaatgaaa aagtggtgtg ctctttattt tgttttataa
     1081 taatagtgag aaaacctatc actactacaa atcacgggga ggtgaataag tgagtggtat
     1141 agccactacc tcgcattttt ataggtgtca ttctttccat caagcacaag aactgtgact
     1201 aggagcacgt aaacttataa ttgaataagc gactcgtttg tgaaaggaaa gaatagtcaa
     1261 gggataggca gactcaaatt tggtgaaggg aaacccctca tgttaaaagc caaacttcaa
     1321 ttttaaaaat tttaaaattt gtttcgtatt cactactcct tgcctttata ttggattgat
     1381 taagagaacc tttcgcgttt ctagaaacta cagtggacta ttctggattc aacgcattat
     1441 agttgaattg actaggaatt ctctttcttt tcaggcttct cttctgattt ttttctccgc
     1501 tttttcgctt gaatcagtcg ctacctcttc ctttttatcc gttttctctt ctttgatttt
     1561 gactgaagga aacagaaact catattgact cttatccgta cgaacagtcg tcaccaagct
     1621 tgttttcttg ttttgatagc tcacctgacc tagattaaaa acctgttccc cactttcttg
     1681 ctcggcttta tccttggttc gtttagccat ggcaaaagca accaattttt ctttatttaa
     1741 agcatagtct tgatagtggg cgatttgtcg ggtcaaataa aattgcaaca aaagactaaa
     1801 gatggctgct atggtgactg cgtagaggag aacacctgcc ttaactttct tctttttcca
     1861 cacgatagat gaactccctt tctaagcctt tttgaaactg gaaacgaaaa tgaaccagtt
     1921 gattgtcctc tgtaatctga gctgatttga ggccataaac cataggctga tacccccgtc
     1981 cactagcatc ggttttacga aaatcgtctg actttgactt accgatggcg atgtccttgt
     2041 catcttgctt catgtatagg cgattgcctt ctactttttc gaactgcgaa cggtctaatt
     2101 ctgcctcaag ttggcccaca aacaagagcc actccttttg ctcgctttgt tgctggtagc
     2161 gaacttctga aatgaggagc tgactcatag cttgaaaaag gagtaatccc ccactgatga
     2221 caatgagggc aagcagggat tctaacaagg taaaagcctt gaccttatgg ctctttgatt
     2281 gccaacaact gttctgaacc atggtagacc tccaatccct tttcactaga aaatacctga
     2341 atctcaactc cgttgatgct tacctgattt tgccccgtct gcaaggccat cttagctacc
     2401 ctcaagactt cttccttttg caagattttt gcttcctctt gcctattttt ttgaatttgt
     2461 cccaaaagga gggtcgcaat gctggcaaag atagctagag cgactactgc ttccagtaaa
     2521 atcactgccc taattttttg tttccttaat gcgtttaatt tttccatttc ctagatataa
     2581 ttgatagcga atcgctcctt tactggtctg aaattcaacc ttagccaggg acgaattgcc
     2641 cccagctcgg tcaaatgtaa tactttggcc tgatggtgcc tgaattcctt taggaactgt
     2701 caacttttga ctgccattgc taatcatctg cccatccaag ttcagactag tcttttgttg
     2761 actagctaca ctgcgttttt gggtttcccg atagagttct tcaaactcca taaagaaaat
     2821 ctgttcctct accgctgcaa aagtggactg gacagagccg gacaagccca aggcaaggat
     2881 actcacaagt cccaaaacca agagactttc cagcatggta aaggccttaa tcattgactt
     2941 tacgatttgc ccctccattt ttagtatagt attcattata agctttaacc tgttcttccg
     3001 tgatgcgccc atctccttgt aacttgctca gagtagcatc ttcattttta tccaagctat
     3061 aaagttctgc ctggctttcc accaccttaa caacagctgc ttttcctttg tcattgactg
     3121 cttctttttg cttggtcaga ttaggtacaa agagcaagaa aagcacgctg ataatcagca
     3181 agaccaccaa catctccacc aatgtaaaag ctttaacctt agcttttttc aagaatgtca
     3241 tcattttttt cattttaaaa atttacctcc atattttgat acatgggcat gagcattgcc
     3301 gcataaagta aaacgataat cagtgccaca aagataaaaa ccagtggctg caccaaattc
     3361 atggtgcggt tgactcgggt aaaaaaggct tcccaagttt tttcagcata gatttccaac
     3421 tcactaccca gcttggactt aacttcccca tactctatga tgagactcaa ttccttccta
     3481 aagaaaggat aggttcctat cgtctgagaa aattcacggc catttttcag ggtttgagcc
     3541 agatcttgac cgatttcttt aaagagctgg gaaccttgtt cctgcatcat ttgaaaaatc
     3601 tgcgtcagct ccattccctg tgaaatcata ttcccccatt cacgtgcata ataggctgtc
     3661 aagtaggtct gcacaaagat tccaataaag ggaaggcgtg ctaagataga aaagacactc
     3721 atcttagaac ttcttttata aaaagttagt gctaaaaggg caagcacgga aacaagccct
     3781 accatgccta gaaaaatttg tggcagatta ccgataattc gggtggcaat attgctacta
     3841 tccagttgtg ggagcaggta attccgtagc cccagcataa ttaagagaag aaaatccagc
     3901 aaaatcaaag gataggtcgc tacttcaatt aactttttct tgaccttggc cagattgtct
     3961 agatattctt ctatctttcc caaactcagg tgaagattgc catgaacttc agctagagat
     4021 aactgagtga caatggtact tgaacatccc aaactttcca tcatttctga gaatgatttc
     4081 ccctgagaca agcctgtacg catctgggtc acacactgct tgtccaacaa ggaactccta
     4141 tctaaaaagg agatagtctc caccagatga aaaccgctag aaaagagatt gttaaatagg
     4201 gtgatgatat ttttttgctt agctgtagct aattttttcc gtctcagcct gaagacttgt
     4261 gatatgtcca tctttaagaa gttggtcaat ttgctcattc cacttggctg cttggtgttc
     4321 ttgataatct ctgtttgcaa agtcaacgat tcctcctccc ccgattaatc tctggtagca
     4381 gactccttgc agaacaactg ccaattcttc ttcactcaca cccaactcca gcagacgctc
     4441 ataaacacct cggatactct tggcgtgaat ggttgaaaag actgtcgcac ctgtcaaact
     4501 agctctgacc actgcacgcg ccgtctcgct gtcacgaatt tctccgataa tcaagagatc
     4561 tggtcgatga cgcaaggaaa gtttgattag attttcatag gttaggccga ttgcttcgtt
     4621 caactgcaac tgaagcatgt cgtcctgctt gatttcgaca ggatcttcga tggacataac
     4681 ttgctgtcct ttaaagagtg acttggacaa ttcatgcatc aaggtcgtct taccactccc
     4741 aaccgggcca gcaaaaagat agagtccccg ttgcctgtac tgcttgccta attcttcaat
     4801 atcctgaaac caaaaatgca agtcctgctc ctcatcgtgc aacaaacgga taaccaaact
     4861 ctcatgcccc cgataatcgc ctacagtaga taaacgtaga gacgccatct tctggtcata
     4921 ggcataatca caggaaccca gttgactacg tcttttttct cccacattca tacccgccac
     4981 aaacttaaag tgactgataa cggctgcaaa cttttcaaaa tcatagctac caattttaca
     5041 gcgctcgtct cctaccctca tatgaagctc ataggcgtct aacttaggga caaaataaat
     5101 atcctgcgtc ccttttttcc gagctgaacg aatgatttct tgtgcaattt cttgaaccat
     5161 acttacctcc tcacctatac tattcgcaaa gcgttagaaa aataaaaaaa gcaactccaa
     5221 aaaagttact tttctctatt ttaatttcat acggcaagaa cgattccttt cctgactggt
     5281 ttgtttttca tagcctgagc gtagtttttc gtctgggata acctgctcat cctgcaaaag
     5341 agccaaagaa tggtattgtt gtaaatactc atcacattca tcacaccagc gttggtcttc
     5401 cgggtcccaa aaaatggtca tcaagtcgct tctactttta taaagagggg attcttgttc
     5461 caatctaaac cagcaagttt tgtagtattt gagagcatca taatactggt caaattttct
     5521 acttgctaca atatcttctt cccaaccttc taagaaccac cacggttcaa aatctccgta
     5581 catttctata acacgataca tattcattca ttcctttgta ccgtatatta taaccaattc
     5641 cgccagacaa ggaaagaaat atgctcattt cttaattttt tgataaaaaa tccagccatc
     5701 tgatggatga ctgaatttct atttgcttat gtacaaaaat atttttacta cttaggcttc
     5761 tgagatatcg ttttcgataa tgaccttaat ggcatggtgg tctgctgcct tactgaagac
     5821 ttcgtaggct ttttcaattt cactgagttt gaaatagtga gttaccaatt tttccggttc
     5881 aatcttatga ctttcaagtg ctttcaacaa ttgtggagtc gtatttgtag ataccaaacc
     5941 agttgttaca ttgatgttgc gaatccaaag tttatctaaa tcgaattcaa ctggtttacc
     6001 atgcacacca cagttggcaa ccgttccgtc tacaccgata atcttttgac agaaatcaaa
     6061 tgtttcagga atccctacag cttcgatagc gacatccaca ccacgaccat ctgtcaaatc
     6121 ataaatttct ttaatggctt ttttagggtc tgaagaatta accttatgag tcgcaccgaa
     6181 tgatagggca gtttccaagc ggttatcgtc taggtctacc ataatcaatt tagctggtga
     6241 atagaattgg gctgttaaaa gagcagccaa tccaactgga cctgaaccaa taatggctac
     6301 gctgcaacca ggttctactt tcccttttaa gacaccaatt tcatatccag taggcagaat
     6361 gtctgacagc ataaccaaag cttcatctga caagtcttct ggagtatggt aaagagtatt
     6421 atctgcatga gggacacgta gatattcagc ctgcatacca tcaatcaagt gaccgaaaat
     6481 ccagccccct tcgtcttcac agtgggcata aattcctttt ttacagtagt agcatttacc
     6541 acaggcacag acgcaagaaa tcaagacctt gtcacctttt ttgaagttgg aaactccttc
     6601 cccaacttct tcaacaatcc caatcccttc gtggccaaga atggtaccac tttggcaagt
     6661 aggaacatcc cctttgataa tatggaggtc tgttccacaa atagtggttt ttacaatacg
     6721 cacaatagcg tctgttggct tgcgaataac tggtttgtct acatcaacaa aagaagcaag
     6781 tcctggttta acataagtat aggctttcat aaagcactcc tccgtttttt tatttgtaat
     6841 atttatagtt caattgtaag cctttttctt tgtttcttca agcttttttt gtgatttttt
     6901 taacttttta tttaccagta aagtaccctg tataaaaaag cagaagctag tctctaactt
     6961 ctgccttctc tcttatgctt gataacgttt cacaccatct aggtaggttg ctaccaattc
     7021 caaatcttta tctaatacga tgaagtcagc gtcgtagcct tcacggattt ggccacagac
     7081 gtcatcgatg tgaacggatt tagctgggtt gaagctggcc atcatgactg cttcatgcgg
     7141 attcgcaatt ccccattcga ccacattctt caaaccatct ttgagtttga ggatagaacc
     7201 tgccaagtta cctgtcgatt tgaggcgtgc agttccattt gcaacgacaa ctgggaattc
     7261 tcccaacata taatctccgt cttccaatcc cccagctgtc atacagtctg tgataagagc
     7321 gatgttttct gttccttttt gtttgataag aatttcgcaa gcctttggat ctacgtggtg
     7381 accatcacag atcaactctg cataggtatg tggcaattgg tacatggctc caaccatacc
     7441 caattcacgg tgagtcaacc cacgcattcc attgtaggca tgcacccaaa cactcgctcc
     7501 agcatcgact gcttttttgg cttcatcaaa agtcgcgttt gaatgtccaa gagcaaccgt
     7561 cacaccttcg cccgtaactg tacgaacaaa gtcttccacc ccatcacgtt ctggtgcaag
     7621 ggcaatttta ttaagcaagc catttgccgc tttttgccaa gaatgaaact cctcaacacc
     7681 cgggtctctc atataagttg gattttgtgc ccccttaaaa gtttctgtga aatatggacc
     7741 ttcataataa atcccacgaa tcttagcacc tgttgcttct ttataatggt ttccaagatt
     7801 ttcagtgact gcaagcaatt gctcataagt ggctgttaaa gttgtgggta agaaactggt
     7861 aacaccggta ctaagaagtc cttcactcat agtatgcaat gtaccttcaa tgttgttgtc
     7921 catcacatct acacctgcat atccatgaat atgagtatcc acaagtcctg gggcaatgct
     7981 ataacctgta tagtcaatca cctcagcccc ttcaggaatc tgctctacat gtttcccaaa
     8041 cttgccgtcc acaagttcca agtaaccacc tcgacgaact ccgtgtgggt agaaaaactg
     8101 atccgcttta atatagttag gcataatgtt aacctcctta aaagattgat tctacaattt
     8161 attatgtcaa ttacattata aacctttctt ttttatttgt aaatggtata gacctatttt
     8221 ttggagatat tttaaaagag ctggatttct ccaactcttc tcttttttaa tacaagttat
     8281 tttgatttaa ccggcttgtc ttgagctgtc tgcaaagctg tggcaatcgt atctgcatac
     8341 aattttgctc ctgcttcgat agtgctactc tcactcccga aatgaacctg gtctgttcca
     8401 gcccaaattt ctggatgctc tttcgcaact tgattccaat ctgctatcgt aatgtaaggt
     8461 gtcttctctg ccaattctct catataggca gcagccttct caacgatggc ataggtctct
     8521 tttgtcttat ctccctcata aggagtcacc aaaatcatat ggtgtccctt aggaagattt
     8581 ttcacgatac tgtcccagtc atccttgtaa ttctcaggat tatttacccc agtcgcaatg
     8641 accaccgtct taggtaaaaa tttattctgg ctattattta gcatgatttc atttgcggtc
     8701 ttggttgtta cgctgacctg cgcgttaatc tgtgctccag gaagagctgt ctgtagtgct
     8761 gtatttgccc ttaaagccac tgagtcacca attaacatag tgccatcagc aattcccaaa
     8821 ctgtttgcat ctgcccgttc tgccatcacc ttggtctggc caatatttgt tgcagcttgc
     8881 ttcaagccat tgacagtcaa gtctgtctca aacgctccca cttgtggtgc caacaaggtc
     8941 accatgcaga caatgaaggt caagattcct gtacctgctg caagaattgc gtgaatataa
     9001 ggcaggggac gaagagtttg taaaataggt gtgttcttgc ctgcaatcca aggttccaat
     9061 acataaaatg acagactggc aaagccataa gaacaaatca gagtcagtaa tacagcaaga
     9121 agatttgatg tcaactgtga gaaaatgata tagaaaggcc aatggaaaag ataaaccgca
     9181 tagctagtat ccgctaaaaa gctgataatc ttcggctcct gtatatgatg tgtcttttca
     9241 tgtaagacac gcgccgccag aatcatggca agagctgcaa gactggcaag caagaagccg
     9301 ataagatagg caaaaagata agtgaatttg acaaagaaag tcaaaagaac taggaaacca
     9361 aagcctcctc caaaaactac taaagtcttt cgtaaatccc agattttatc caactgcttg
     9421 acgagggaag tcgtctgacg aacgcctaca atagttgcta acatacttcc caaaaagaat
     9481 ggatagacat gagttaaact ggagaaataa acagaggaat aagaggtcac tagaaaacta
     9541 ccaataaaca tggagaagaa actgatcaag aaggcagtag cagataagag aaagatcatc
     9601 cccttcaact gaccatttga tttagcttgt ttggataaga accaaactgc caatccccaa
     9661 agaatatagt agtgaacctc aactgccaaa ctccaattat gaacaaacaa atgaggaatg
     9721 aactgagatt cataactccc acctgttaag agttcataga agttggtcat aaagcctaag
     9781 acgcccgcaa tctggccacc aattccagca acatagtctt ggcgaaccaa gaaagtaaaa
     9841 ggcatggtca ccaagaccat caaaaccaca ggtggcacaa tccgataaaa gcgtcttcta
     9901 aaaaatccta tcaaatcaat ctcattgttc ttagaaaatt cttcaatgag tagagctgta
     9961 atcaggaaac ctgaaaatgt gaaaaagaca tctaccccga aaaatcctcc aggaaagatc
    10021 gtctgaaaga agtgatacaa gagtaccaaa agtaaaccta taatcctaat caaggaaaac
    10081 catttaatgc gcatacgagt ttattcttcc tttcattgta cactttattc tatcaaaaaa
    10141 agaagaaaaa tcctaagaga aaacttagga tttttagctt atatcaattc cattttagaa
    10201 attacgtcct gacttattat agccatattt ttccacaaaa tacttacgga attccaagag
    10261 attgtcatcc atgatggctt gtcgcacttg cttcatcagg ttaagcaaga agtaaagatt
    10321 gtggtagcta gtcaagcgga taccaaaggt ttcatcagcc ttgagcaggt gacgaaggta
    10381 agcgcgtgta tagttattac atgtgtagca atcacactca ggatccagtg gcgtaaagtc
    10441 ctcagcaaac tgggcatttt tcacaaccaa acgtccttga ctggtcatac aagtcccgtt
    10501 acgagcaatt cgagtcggta agacacagtc aaacatatcc accccacgaa tgaccccatc
    10561 gatcaagcta tctggcgctc ccacacccat cagataacga ggtttatttt caggcagcag
    10621 ttgagttgta aagtccaaga ccgcattcat ctcttcatgg gtttctccca ctgccaaacc
    10681 accgatagag tagcctgaga aatccatgct gacaagatcg tgagctgatt ggcggcgaag
    10741 gtcttcaaat cctgcacctt gcacaattcc aaacaaacct tggtcatgtg gacgacggtg
    10801 agccttcaaa ccacgctcag cccaacggct ggtacgctcg atcgatttct taacgtagtc
    10861 ataaggttga taaaactgag gacattcatc aaaggacatc atgatgtctg aacccagatt
    10921 attctgaata gagatggctt tttctgggga taggagcatc ttagaaccat ttagatgatt
    10981 tttaaaggtt actccttctt ctgtgatatt acggctatct gctaaagaat aaacctgaaa
    11041 accaccacta tctgtcaaga taggctggtc ccaattcatg aacttgtgga gaccaccagc
    11101 gcgtgcaatg agttcatctc cagggcgaag ccagagatga taggtgtttg ataggataat
    11161 tcccgaaccc atctccttca attcttcagg tgactgagtt ttgacagtgg cttgtgtccc
    11221 aactggcata aacataggtg tcggaaaggt accgtgggga gtgatgattt ctcctagacg
    11281 agctcctgtg tgtttttctt tcttaatcaa acgatatttg attggtgaat ctgacatttt
    11341 ttacctccga agctgggaaa gacagtccca gttcatacag tgtgcccaag gacacactgt
    11401 atgattttat caaaaaaagt aggaactgtc acgaactatg tataagaact atggtataat
    11461 aagagaatga actatccaaa aattgattta aaaacaattc gtcaggagtc aaaacatttt
    11521 caggctgaca ccccccgtct ctttctcctc tatatccttc ccagtatgct ggtcatctta
    11581 tctggttttc ttaatccctt atctcggatc cacgggactg ttttagaaca gccatttttc
    11641 agcatacttg gccaaatact tcaaacctac ctctttcctc tactagtctc ctttatcggg
    11701 actattcttt tgaccagttc cgtctatgca acactgacac tcatgaaaga ttctaaaacg
    11761 gagccatctg taaaaaacag cctagctctc tttgatgagg agcgcttttc tcaaaccttt
    11821 ttgaccctac ttctcaaacg tttctatctc tttttatgga gtatccctaa tttacttggg
    11881 atttacctcc tcttttatag tagcttttta gccaaaaaat tcgtcaccct acatcctgag
    11941 tttccaaatc tagatctctc atcagttgaa accgagcgtt tcctcatggt ctttggactc
    12001 tactttcttg caagtctcat cttgatcatt gtaggaaata tcctctatat tccacagtat
    12061 tatgcctatt cacaggtgga atttcttctc tgttatagtc tagacctagg acaggtccca
    12121 ccaagacgaa tcctaaaaac tagccgttcc tttatgaagg gctacaagtt ccagcacttt
    12181 gtcctagatt tacagcttct tccttggtac ttcctcaatt ggattacctt tggcattgct
    12241 agtttttcac tcctacctta tatccaatgc actaaaatta tgttttaccg agcagtattg
    12301 gctcgaaaac gtccaaaagc ttgaaggttt ttcccctcaa gcttttttct atcaatgagt
    12361 ttctccgcct accaacttga gttcctgatc tccatgttct atcatagcgc cgattgctgc
    12421 ttctatccct cgccgaatat ccactaaact catagttgga gtagtcggtc tgttcaccac
    12481 ctgttccatc atataaggaa tatgcataaa acctgcctta acatatggaa atttcttttc
    12541 taccaaatag agagcctgat acatcaaatg gctgcagaca aaagtccctg ccgtattgga
    12601 aacagaggcc ggtaagccct ctttttttat agcttgaacc atcgctttaa tcggcaaact
    12661 actaaagtag gccgaagcac catctgggcg aatgggacgg tcaatcggtt gattatcttc
    12721 gttatcagaa atgcgtgcat cgtcttgatt aatggccact cgttcaagtg tcacactagt
    12781 tcttccacca gcttgcccaa tacaaaggac aaagtcaggt tgataacgat tcatctcttc
    12841 ttccaatact tgagcagatt tgtgaaaaac tgtcggcacc tctagccaac ggacctcagc
    12901 accatggatt tcagctggta aacctttaat ggcctccaaa gttggattgc ccttttcccc
    12961 tccaaagggc tcaaaacctg tcactaatac tttcatttct aactcctcaa aaccaattca
    13021 atgagacttt tttcttaaaa acaagtataa catatttccc ttattctgga ttaaaaagga
    13081 ttagcgagat taaaatcgac cttcaagtgt atttaaagag taaaatacta atcaaggcca
    13141 aaatagctga tccaccttgc ttcaaaataa tttttttatc cgctgttaaa gagccgtaag
    13201 tcgcagcacc aatcacaaat aagacaaaaa tagtcacaat ttctaaattc tgtgagaaat
    13261 aaatgccata ccagagaaag actcctagca gagccttata aatcccttga tttttgaaca
    13321 atgaacttac tgacggatga gccaattctt ccttttccat attaaataca cgactagtcg
    13381 catctgatcg cgtagcaata ctttccaaat aaaaaatgta aaaatgctcc aaagcaacga
    13441 tcgttgttaa aataattgta ataattgaca tctatttctc cttaaatttc tatattttca
    13501 atacccaaaa ccaatttatt taataaacgg ctgagttctg ttctctcaaa ctctgttaag
    13561 atactttcca tctcttcctt aaccttgata tgttgcagag ggggattcac cactaactgc
    13621 tccttggcat acttcgcagc ctctaccaac acttcccgct gattttcagg attacgatga
    13681 cgctccacca aaccttccgt ttccaaaatc ttgaaatgcc gtgtcaaagc agcctgatca
    13741 attttcaaac gctcctgaac cgccatttgg ttacaaggag aatgctccag caaaaacagt
    13801 aaaatctgat accgtgtcaa actaatccca agcctttttt caaacaattg cgtactcgct
    13861 tgctcagaca agcggagttg atacagtaaa tcttgaatct ctatcatttc ttcctcctat
    13921 ccattgactt atcaatagtt gaccaatcaa ttataatata aagtatagtg aaatgaaata
    13981 agagcaggac aaatcgatca ggacagtcaa atcgatttct aacaatgttt tagaagcagt
    14041 tgtgtactat tctagtttca atctactata aaatagattg caagaatgtt gcttagatta
    14101 ttacaagatg ataactttcc attttccatt caaattgata ctcaatgaaa atcaaaaag
//
LOCUS       DAVBCN010000054        12469 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVBCN010000054 DAVBCN010000000
VERSION     DAVBCN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12469
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.054"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(251..433)
                     /locus_tag="VQ497_001492"
     CDS             complement(251..433)
                     /locus_tag="VQ497_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517788.1"
                     /translation="MAVIFKNVLIEEKTEEEKQHILSILNGEYDLSDDVSCRLRRLNE
                     ESKRLEVFVKETNSLV"
     gene            complement(649..1881)
                     /locus_tag="VQ497_001493"
     CDS             complement(649..1881)
                     /locus_tag="VQ497_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET2517789.1"
                     /translation="MKIAVAGTGYVGLSIAILLAQHHQVVAVDIIPEKVELINKKKSP
                     IQDDYIEKYLAEKELNLVATLNAEEAYENADFIVIAAPTNYDPKKNFFDTSSVEAVIE
                     AVMKYNPDAIIIIKSTVPVGYTKSVRRKFLTDNIMFSPEFLRESKALYDNLYPSRIII
                     GTDKDDKDLVKSAEIFVKMLQEGAVKENIETLYMDFTEAEAVKLFANTYLALRVSYFN
                     ELDTYSEMKGLDTQAIIDGVCLDPRIGSHYNNPSFGYGGYCLPKDTKQLLANYSDVPQ
                     EMITAIVQSNRTRKDYIADRILDLAGSYVGNDFYKPDKEKEIVVGIYRLTMKSNSDNF
                     RQSSIQGIMKRLKAKGVTIIIYEPTLESGTTFYGSQVVNDFEIFKKSSQVIVANRFDE
                     TLEDVRGKVYTRDIFKRD"
     gene            complement(1910..2980)
                     /gene="gla"
                     /locus_tag="VQ497_001494"
     CDS             complement(1910..2980)
                     /gene="gla"
                     /locus_tag="VQ497_001494"
                     /EC_number="5.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate 4-epimerase Gla"
                     /protein_id="HET2517790.1"
                     /translation="MRGKTILITGVAGFIGSNLVRKLLELEGTMTIIGLDNLNDYYDI
                     ALKDYRLLQLNKLIQDYPEKNWIFIKGDIADKMLINKIFKSYHPQIVVNLAAQAGVRY
                     SITNPDVYIESNIVGFHNVLEACRHSYEIYDGGVEHLVYASSSSVYGGNKKIPFATED
                     KVDNPISLYAATKKTNELQAYAYSKIYNIPSTGLRFFTVYGPAGRPDMAYFGFTDKLR
                     SGSDIEIYNHGNCKRDFTYIDDIVEGIVRVMQSAPKKLVGSDNLPLAPYKVYNIGNSK
                     PENLLDFVDVLQQELIKAGVLPENYDFDSHKKLVPMQPGDVPVTYADTSDLERDFGFK
                     PKTSLREGLRKFAEWYKDYYNV"
     gene            complement(3048..4217)
                     /locus_tag="VQ497_001495"
     CDS             complement(3048..4217)
                     /locus_tag="VQ497_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET2517791.1"
                     /translation="MRLSKKYWFALTSKFLLILLTLLSSIIINRGLGVEKKGDYAYVM
                     NLVEIFYILGGVGLGQAYSTFKRNHSNEYQNKFVFLTFIQGLLFGIIGLIIVLADVPI
                     DYGGVIIVLSSLSIMRTNLTMIAVIENSIKRNKIVLGVSVVYSVMLVLLLLFHLVSIE
                     MVFLVYAINEFLRIILFLYYYNMFPVFEHISIKEFFTIYKFGFLSMILTLLITFNYSI
                     DIIMLKQMSSSYNVGIYSVAVTFSNMFLMVPDSFKEILFGDSAKRTFEKNKAINAIKV
                     SFLFMILLIIGFLSFGEFAITLLYGSSYIKAFPITLVIFLGSLSMIFFKILQPIYISQ
                     GKQSVAIKILSVSALINISLNWYLIPNYQITGAAIASAISYTVCGLFFIIDYLRN"
     gene            complement(4403..5707)
                     /locus_tag="VQ497_001496"
     CDS             complement(4403..5707)
                     /locus_tag="VQ497_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HET2517792.1"
                     /translation="MKFSLQSNDIKNKIILLVGVVFSGTLILCSENNDVTIPLFSMIF
                     LSFMAMIIVKFNIFHPYVWYSSFFTIYSISYPLLYRMEYINYGYTKDLIFMQWLALAT
                     LLLTLPSKAIVLSERKINLNNNNYLLFLNNMNTFITTVAIIYLLGSGFRNKGEIYSGA
                     NIVIMLVFSLVYFMILIHTYQLFIQLSTKKWRLLNCNTNTAIVICLFSIVTGERDYMF
                     TLFILDVIILFYFHKIKRFYLVMLVPLAALLIPLSAVFKYTLLSGQVSSINTNNIWFD
                     LLDGEFVSASRNLQILILHNMGNYFEGRSFFNDIVRIFYNTGYSNQTWFMDTFFPNVH
                     STKYGFTLVGEGYVNGGYFGIVMIFMLTGFLMRFLYINAQRNIYGMLIYLYMIPIFIY
                     STRADFANILSPLLKYAILGTLVIVFINRMVLISPLDEMGGE"
     gene            complement(5824..6978)
                     /locus_tag="VQ497_001497"
     CDS             complement(5824..6978)
                     /locus_tag="VQ497_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2517793.1"
                     /translation="MKVFIATEFRCTIYKNEIYLKPKAYFIYERYAKAFGEIVLCSRY
                     EKVESYPEGYHKANFINKFIPIEGLHQVLFGQNKNKIIEGMIDSDLIVVRIPSIIGSK
                     TADYALKIGKPYLTEIMGDAWDSYWYHSLKGKLLAPYIYAKTKSIVKNANYCIYVTEK
                     YLQDRYPNIKSNIVASNVNITSVENRSLKSRLYKLKKFNPQKISIMTTASVNVRAKGH
                     RFVLEAMKRLEIQGILLDYYLAGDGDQSFLKKKAEELGVANRIHFLGELTTSQIYEYL
                     DKVDLYIQPSLQEGLPRAVIEAMSRACPCIGSNKAGIPELLGPHCLFTPSSSQAIAGS
                     IIDFISMDKEKMVIKQFEFSKKFLSSVLNKRRSDYFAQIRRELNNSDGLD"
     gene            complement(6975..7526)
                     /locus_tag="VQ497_001498"
     CDS             complement(6975..7526)
                     /locus_tag="VQ497_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET2517794.1"
                     /translation="MNRIIKNIVGLLRANICLILLKVCRNKDLTFHYFLKFGKNVTVE
                     QESNAKLILGKKIRVNAGGVLKVRKGAKLKISDDVFLSNNCMIACRKYIDIKSGVKCG
                     PGVLIYDHDYDVSVPGGLKAKKFKTAPVMIGENVWIGANSIVLKGVSIGENSVVAAGS
                     VVTKDIPADTIFIQKRLSREMKL"
     gene            complement(7519..8655)
                     /locus_tag="VQ497_001499"
     CDS             complement(7519..8655)
                     /locus_tag="VQ497_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2517795.1"
                     /translation="MNLQKKKIMLITNHDDDIYCFRKEVIESLVEYGYEVLVSCPYGD
                     KIELMKSIQFEYEDITIDRRGINPLKDIRLFLSYIRLLRKYRPDVVLTYTVKPNVYAS
                     LAATILGIKYINNVTGLGSVLTMGSCVRSLVLLLFRISFRKSNCVFFQNEYNMKMLKT
                     LGLILGDSKLIPGSGVNIEKYPVQQYPNGGNGIQGETIVFNFIGRILKEKGIDTYLAA
                     AQIIKSRYPKTEFNIIGFIEPTESNYELKICDLEKKGIVYYLGQQKDVIPHITRSHAI
                     IHPSVYGEGMSNVLLENASSGRVLITTDNPGCKEIVKDRETGYIFQGGNVEELVSILE
                     VFLGLENEKRKEMGLQGRMYIEQYFSRQIIIDAYLDKIKGALDE"
     gene            complement(8796..9461)
                     /gene="cpsD"
                     /locus_tag="VQ497_001500"
     CDS             complement(8796..9461)
                     /gene="cpsD"
                     /locus_tag="VQ497_001500"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HET2517796.1"
                     /translation="MPTLEIAQKKLEFIKKAEEYYNTLCTNIQLSGDKLKVISVTSVN
                     PGEGKTTTSVNIAMSFARAGYKTLLFDGDIRNSVMSGFFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVVQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTPPIGIVI
                     DAAIITQKCDASILITATGEANKRDVQKAKQQLEQTGELFLGVVLNKLDISVDKYGVY
                     GRK"
     gene            complement(9471..10163)
                     /gene="cpsC"
                     /locus_tag="VQ497_001501"
     CDS             complement(9471..10163)
                     /gene="cpsC"
                     /locus_tag="VQ497_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HET2517797.1"
                     /translation="MKEQNTLEIDVLQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYVVNRNQGEKPGLTNQDLQAGAYLVKDYREIVLSQDVLEEVISDLKLD
                     LMPKGLANKIKVTVPVDTRIVSVSVSDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPAISPSSPNIKRNTLIGFLAGVIGTSVIVLLLELLDTRVKRPEDIENALQMT
                     LLGVVPNLGKLK"
     gene            complement(10172..10903)
                     /locus_tag="VQ497_001502"
     CDS             complement(10172..10903)
                     /locus_tag="VQ497_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase CpsB"
                     /protein_id="HET2517798.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYRQIHTGLSNILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QINSYHVSKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKAKELFVDNPRKIIMDQLI"
     gene            complement(10905..12350)
                     /locus_tag="VQ497_001503"
     CDS             complement(10905..12350)
                     /locus_tag="VQ497_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET2517799.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLICFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLLFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSKIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADS
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLTDGDRDRGRNQQKVIVAIIQKLTST
                     EVLKNYSSILQGLQDSLQTNIPIETMIDLVNTQLESGGNYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLTVVKAAIQDVMEGR"
BASE COUNT         4192 a         2422 c         1640 g         4215 t
ORIGIN      
        1 aattcactta cttcatatta catttagcat gaaatgaaat aagcgaattc tactttgttt
       61 gcatacaatt aaaaatcaat tcctaaggat gttttaaata actgtgtaaa aggcataatt
      121 tcaccatttc ttagtttttc aatgctaaat tacatagtaa ctggagagct attagaaatc
      181 tctaacttcc tcatagttaa aaaatgacaa tgagatactt attgatttca attcattaac
      241 agaatacgaa ctagacaagt gagttcgtct ccttaacaaa cacttccaag cgtttacttt
      301 cctcattcag tcgtcgtaat ctacaactta catcatcaga taagtcatac tcaccattta
      361 gaatagatag aatatgttgt ttttcttctt cagtcttctc ttcaataagc acatttttaa
      421 aaattactgc cattatcagc acctcacttc gaaggaatat atgcaactat atcctattaa
      481 gtcaatctct actgacaaaa gaagtatatt tacttcacta agtgcgagcg agaatataga
      541 aatatcttta ttgttttaga tgagttgtcg ctatcctcca gctagttttt aactattcta
      601 tcattcaaat gatatagatt attagggact agaaatatac aaattgattt aatctctctt
      661 aaaaatatcc ctagtataaa ctttacccct cacatcttct aaagtctcat caaaacgatt
      721 tgcaacaatc acttgactag atttcttaaa tatttcaaaa tcattaacta cttgactacc
      781 atagaaagta gtaccacttt caagagttgg ttcgtaaata ataatagtaa caccttttgc
      841 tttcaaacgc ttcataatac cttgaataga agattgtcta aaattatctg aattactttt
      901 cattgttaaa cgataaattc ctacaacaat ctctttttct ttatctggtt tatagaaatc
      961 attgcctaca taagatccag caagatctaa aatacgatct gcaatataat cttttcttgt
     1021 ccgattactt tgtactatag ctgttatcat ttcttgtgga acgtcagaat aatttgcaag
     1081 aagttgcttg gtatctttag gaagacagta acccccataa ccaaaagaag gattattgta
     1141 gtgagaacct attctcggat ctaaacaaac tccatcaata atcgcttgag tatctagtcc
     1201 tttcatctca gaataagtat ctaactcgtt aaaataggaa acccggaggg caagataggt
     1261 attagcaaaa agttttactg cttcagcttc tgtaaaatcc atatacaatg tttcaatatt
     1321 ttctttcact gcaccttcct gaagcatttt tacaaaaatt tcagcagact ttaccaaatc
     1381 tttatcatct ttatctgtac ctataataat tcgactcgga tataggttat catataacgc
     1441 ttttgactcc cgtaaaaact caggactaaa cataatattg tctgtaagaa atttcctccg
     1501 tactgacttt gtatatccaa caggaacagt acttttaatt attataattg catcaggatt
     1561 atatttcata actgcttcaa tgacagcctc gactgaagat gtatcgaaaa aatttttttt
     1621 cggatcataa ttagttggag ccgcaataac tataaaatcc gcattctcat atgcttcctc
     1681 tgcattcaat gtagctacga gattcaattc tttttccgcg agatattttt ctatataatc
     1741 gtcttggata ggggacttct ttttattgat taactcaact ttctcaggaa tgatatctac
     1801 ggcaacaact tgatgatgtt gagccagtaa gattgcaata gataatccaa catatcctgt
     1861 acctgctact gctattttca taattttttc ctttctcaaa acactctcgt tatacattat
     1921 agtagtcttt ataccactct gcaaactttc tcaaaccttc tcgtaaagaa gtttttggtt
     1981 taaaaccaaa atctctctct aaatcacttg tatctgcata agtaacagga acatcgcctg
     2041 gttgcatagg aactaatttt ttatgactat caaaatcata attttccggc aaaactcctg
     2101 ccttgattag ctcctgttga agaacatcca caaaatctaa caaattttcg ggtttactat
     2161 tcccaatatt gtacacttta tatggggcta acggtaaatt atcagatccc actagtttct
     2221 tcggagctga ttgcatgacc cttactatcc cctcaacgat atcatctata taagtaaaat
     2281 ctcgtttaca attaccatga ttataaatct caatatcgga accacttcgc aatttatctg
     2341 taaatccaaa ataggccata tctgggcgcc cagcaggacc ataaactgta aaaaagcgta
     2401 agcctgtcga aggaatattg taaatctttg aataagcata cgcttgaagt tcatttgttt
     2461 ttttagtcgc agcatatagt gaaataggat tgtctacctt atcttctgtt gcaaacggga
     2521 tttttttatt tccaccatat accgatgagg acgatgcata aactaagtgt tccacaccac
     2581 catcatatat ctcatacgaa tgacgacaag cttccaacac attatgaaag ccaacgatat
     2641 tactttctat atatacatct ggatttgtaa tggagtagcg tactcctgcc tgggcagcta
     2701 gattaacaac aatctgagga tgataactct taaaaatctt attaataagc attttatctg
     2761 caatatcacc cttgataaat atccagtttt tttctggata atcctgaatt agcttattaa
     2821 gttgaagcaa tcgataatct ttaagtgcaa tatcatagta atcattcaaa ttgtccaaac
     2881 caatgattgt catcgttcct tctaattcca ataatttcct aaccagattt gatccaataa
     2941 aaccagctac tcctgtaatt agaattgttt ttcctctcat atcataatac ctcttgttat
     3001 ttttatattt tcgtccacta atacacattc tttaacaact attataatca atttctaaga
     3061 taatctataa taaaaaacaa accacataca gtataagaga ttgctgatgc tatcgcagct
     3121 cctgtaattt ggtagttagg aattaagtac cagtttaaac ttatatttat caaagccgac
     3181 acagataaaa tttttatagc tacactctgt ttaccctgag atatataaat tggttgtaaa
     3241 attttaaaga aaatcatcga taagcttcct aaaaaaatga ctaatgttat aggaaatgcc
     3301 tttatataac tggaaccata aaggagcgta atcgcaaatt ctccaaaaga aagaaatcca
     3361 ataattaata aaatcataaa tagaaatgat acttttattg catttattgc tttatttttc
     3421 tcaaaagttc ttttagccga atcaccaaat aaaatctctt taaatgagtc tggaaccatc
     3481 aaaaacatat tagaaaacgt aactgcgaca ctgtatatac ctacattata actactagac
     3541 atttgcttca acattatgat atcaatacta taattaaatg taattaatag agtaagaatc
     3601 atagaaagaa atccaaactt atatatagtg aaaaattctt taatactaat atgttcaaaa
     3661 actgggaaca tattgtaata gtataaaaat aaaatgatac gtaaaaattc attaatagca
     3721 tataccaaaa ataccatctc tatagaaact aaatgaaata gtaacaacaa tactagcata
     3781 acggaatata ctacgcttac tcctaataca atcttattcc tctttatact attttctatt
     3841 actgcaatca tagtaagatt tgttctcata atagatagac tggacaaaac tattattacc
     3901 ccaccataat ctatcggaac atcagctaaa actattatta aacctattat tccaaacaat
     3961 aatccttgaa taaaagttaa aaaaacaaat ttattctgat actcattaga atgattcctt
     4021 ttaaaggtcg agtaagcctg acctaaacca actccaccta aaatataaaa aatttctact
     4081 aagttcatta cataggcata atctcctttt ttttcaacac ctaatcccct attaattata
     4141 attgaggata aaagtgttaa taagattaac aaaaatttac tcgttaaagc aaaccaatat
     4201 ttcttagata gtctcactta ttttctccgt atgttaaatt ttataacagt aaatttaaaa
     4261 ttatagtata ttgaaataga atatgaacaa aacgattagg gaatttaaag taatttataa
     4321 aaattcttta gaagctgcat tgtactactc caacttcaaa ccactatagt atcaaaaagt
     4381 ataccccacc tttttaaata aactactcac cacccatttc atccaatggt gatatcaaca
     4441 ccattctatt gataaaaaca ataaccaatg ttcccaatat agcatattta agtaatggag
     4501 ataatatgtt agcaaaatca gcacgagtag aataaataaa aataggtatc atatacagat
     4561 atatcagcat cccgtatata tttctttgag cgtttatata aagaaatctc atcaaaaagc
     4621 ctgttagcat aaaaatcatt acaattccaa agtatcctcc attcacatat ccctctccca
     4681 caagagtaaa accatatttg gttgaatgta cgtttggaaa aaaggtgtcc ataaaccaag
     4741 tttgattaga ataccccgta ttataaaaaa ttcttactat gtcattaaaa aaagaacgac
     4801 cttcgaaata attgcccata ttatgtaata tcagaatctg taaattacga ctagctgata
     4861 caaactcacc atctagcaaa tcaaaccaaa tattatttgt atttatagat gaaacttgcc
     4921 cacttaataa agtatattta aaaactgcag ataacggaat cagtagtgcc gcaagcggaa
     4981 ctaacattac aagataaaaa cgttttattt tgtgaaagta aaataatata atcacatcaa
     5041 ggatgaataa agtaaacata taatctcttt cccctgtaac gatagagaag agacagataa
     5101 ctatcgctgt gttagtatta caatttagta gtctccattt tttagtactc aactgtataa
     5161 atagctgata tgtatgaatg agaatcataa aataaaccaa agaaaatact aacattatca
     5221 caatattggc acctgaatat atttcccctt tatttctaaa accacttcct aatagatata
     5281 taattgctac tgttgtgata aacgtattca tattattgag aaataataaa tagttattgt
     5341 tatttaggtt tattttcctt tcgcttaaca caatggcttt agaaggtaga gttaacaaca
     5401 aagttgccaa agccagccat tgcatgaaaa tcaaatcttt tgtataacca tagtttatat
     5461 actccattct atagagtagc gggtaagaaa tagaataaat agtaaagaag ctactatacc
     5521 acacataggg atgaaaaata ttaaatttca caattatcat tgccataaaa cttaaaaaaa
     5581 tcattgaaaa taatggaatt gtaacatcat tattttcact gcataaaatt aaagtccctg
     5641 aaaaaactac acctactaga agtattattt tattttttat atcatttgat tgtagactaa
     5701 acttcattta cttttttcct ttaactaata atttaaacgt aatgcgtatt caaaaatttg
     5761 attggctatg tagtcagttt ttatagcata tttaatatat cgaaacggat tttccatcgc
     5821 attttaatct aaaccatcag aattatttaa ttctcttcta atttgagcaa aatagtcact
     5881 tctgcgttta tttaatacac tcgaaagaaa tttttttgag aattcaaact gttttatcac
     5941 catcttttcc ttatccatac taataaaatc tataatagat cccgcaatcg cttgtgaaga
     6001 ggatggagta aatagacaat gtggccccag caactctgga attcctgctt tattagaccc
     6061 tatacaaggg caagcacggc tcatagcttc aattaccgca cgaggtaacc cttcttgtaa
     6121 acttggttgt atatataaat caactttatc caaatactca taaatttgtg atgtagtcaa
     6181 ttcccctaaa aaatggattc tattcgctac tcccaattcc tctgctttct tttttaagaa
     6241 actttgatca ccatcacctg ctaaataata atccaacaaa ataccttgta tttctaatct
     6301 cttcattgct tccaatacaa atctatggcc cttggctcgt acattcacag atgctgttgt
     6361 cattattgaa attttttgag gattaaattt tttcaactta taaagacggc tcttcaaaga
     6421 tctattctct acagaggtaa tattaacatt tgaagcaacg atattagatt taatattagg
     6481 atatctatct tgtaaatatt tttctgtcac gtatatgcaa taattagcgt tttttacaat
     6541 tgatttagtt ttggcgtata tatatggagc taataatttt ccctttaaac tatgatacca
     6601 gtaagaatcc caagcatccc ccattatttc tgtcagatac ggcttaccta tcttcaatgc
     6661 gtagtctgca gtttttgatc caattataga cggaatacga acaactatta agtcgctatc
     6721 aatcattcct tcaataatct tatttttatt ttgaccaaat agaacctgat gtagaccttc
     6781 tatagggata aatttattaa taaagttcgc tttatgatat ccttcagggt atgattccac
     6841 cttctcataa cgactacata gtacaatctc tccaaatgct ttagcatagc gctcataaat
     6901 aaaataagct ttcggtttta aatatatttc atttttatag attgtacatc taaattcagt
     6961 cgctataaat actttcataa tttcatctcc cttgataaac gtttctgaat aaatatagta
     7021 tcagctggaa tatcctttgt tacaacactt cctgctgcaa ccacactatt ctcaccaata
     7081 ctcactccct tcaagacaat gctgttagct ccaatccaaa cattttctcc aatcataact
     7141 ggggccgtct taaatttttt tgctttcaat ccacctggaa cactaacatc atagtcatga
     7201 tcatatataa gtactccagg accacatttt actccagatt taatatctat gtatttacga
     7261 caagctatca tacaattatt actcaaaaat acatcatcag aaatcttgag ttttgctcct
     7321 tttctaactt tcaatactcc cccggcgttt actctaatct tttttcctaa gattaattta
     7381 gcattagatt cctgttcgac agttacattc tttccaaatt ttaaaaaata atgaaaagta
     7441 agatccttgt ttcgacacac ttttaataat atcaagcaaa tatttgcacg aagtaaacca
     7501 acaatatttt ttataatcct attcatctaa agctcctttt attttatcta aataagcatc
     7561 tataataatt tgacgagaaa aatattgctc tatatacatt ctcccttgaa gtcccatctc
     7621 ttttcgtttt tcattttcta gacctaaaaa aacttccaat atagagacta gttcctcaac
     7681 atttccccct tgaaatatat agcctgtctc tctatcttta acaatttctt tgcaacctgg
     7741 attatctgtc gtaattaaaa cacgtcctga actagcgttt tctagtaata cattgctcat
     7801 tccttcacca tacacactgg gatggataat tgcatgggaa cgggtaatat gaggtatcac
     7861 atctttttgt tgtcccaaat aataaacgat tccttttttt tctaagtcac aaattttaag
     7921 ttcataatta ctctctgtcg gttctataaa gccaataata ttaaactctg ttttgggata
     7981 tcgactctta ataatttggg cagcagccag ataagtatct ataccttttt cttttaatat
     8041 cctaccaata aaattaaaaa cgattgtttc tccttgaatt ccatttccac catttggata
     8101 ctgttgcaca ggatactttt caatgttcac tcctgaccca ggtatcagct tagagtcacc
     8161 caaaatcaag ccaagagttt ttaacatttt catattatat tcattttgaa aaaaaacaca
     8221 atttgatttc ctaaaagaaa ttctaaacag taataacacc aggcttctta cacaagaacc
     8281 cattgttaag acactaccta aacctgttac gttatttata tattttattc ctaaaattgt
     8341 tgcagctagg ctagcatata catttggttt cacggtatat gtcaagacta catccggtct
     8401 atattttctc aataatctta tataactaag aaatagacga atatctttta aagggtttat
     8461 tcctcttcta tcaatagtaa tatcttcata ctcaaattga atagacttca tcaactctat
     8521 cttatctcca taaggacatg acactagcac ttcataccca tactcaacta gtgactctat
     8581 aacttctttt cgaaaacaat agatatcatc gtcatgatta gttattaaca taattttttt
     8641 cttttgtaaa ttcatcaaaa taatcttcct aaaatataat taaccgctac tccattatcc
     8701 tttacaacta tcattttaaa aatagtaatt ttctataagt aattgtttcc aaattgtgac
     8761 actttaaaat tctatacaca tatagaactt acaaactatt tacgaccgta aactccatac
     8821 ttatcaaccg agatatccaa tttatttaaa acaactccca ggaacagttc ccctgtttgt
     8881 tctaattgtt gtttcgcttt ttggacatca cgtttattcg cctcacctgt tgctgttatc
     8941 aagatggacg catcacactt ttgagtgata attgccgcat caataacaat tccaataggt
     9001 ggtgtatcaa caatgatata atcaaaatat ttacgcaatg tttcaatcat atcattaaaa
     9061 tttttacttt gtaacaaggc tgtagggttt ggtgatacag atcccgattg aactacaaat
     9121 aaattttcaa tatttgtatc acataaaccg tgagataaat cagctgtccc agataaaaat
     9181 tctgttagcc ctgtaatttt ttcacgagat ttaaaaaatc ctgacataac tgaatttcga
     9241 atatcgccat cgaacaaaag agttttatag cctgcacgcg caaatgacat tgctatattt
     9301 acggaagtag ttgtttttcc ttcccccgga ttaacagaag taacggaaat tacttttagt
     9361 ttatctccgc tcaactgtat atttgtacac aaggtattgt aatattcttc tgccttctta
     9421 atgaactcca gttttttttg tgctatttct aatgtcggca tctctctctc ctatttcaac
     9481 ttacccaagt ttggtacaac tcccaaaagt gtcatctgca gtgcattttc gatatcttcc
     9541 ggacgtttca cacgagtgtc caaaagttca agaagaagaa ctataacact agttccaatc
     9601 acccctgcca aaaaaccaat tagtgtattg cgtttaatat ttggcgaaga cggtgatatc
     9661 gccggtcttg cctcctccag tgttgtcaca tcagaaacac gagtaatact gataattttt
     9721 tgagcagcta cttctcttaa agagttagcg atacggcttg cctcttcagg aactcgatca
     9781 ctaactgaaa cagagacaat acgggtatca actggtactg ttactttaat tttattagcc
     9841 aaaccttttg gcatcaaatc tagtttcaaa tcagaaataa cttcctccaa aacatcctgc
     9901 gataggacaa tttcacgata gtctttaacc aagtatgctc ctgcctgcaa gtcttgattg
     9961 gttaaaccag gcttctctcc ctgattacgg ttaactacat aaatccgagt cgtactagta
    10021 aactcaggtt tgataacaaa agtactgtag gcaaaagcaa ctgaagaagt tataattgcc
    10081 actaataaaa tgaccaactt tcttttccat aaagctctga atagttgcaa tacatcgatt
    10141 tccaaagtgt tttgttcctt catattttct cctaaattaa ttgatccatt ataatttttc
    10201 tgggattatc tacaaaaagt tctttcgctt ttttcgctcc atatttctta gcaatgatat
    10261 catatgcctg ttgcatatat ggaggtctac tgtctaaatt gtgcatgtca cttgcaacta
    10321 catgaactaa atcacgttcc aaaaaatacc gagctctctt tttcatgaat ttatattttt
    10381 caccaaagaa cttaggtttt gaaacatgat aactatttat ctgagtatag caccccatat
    10441 caatcagttc acgaacacgt ttttcgttat tctctaaagc atcataacgt tcaatatgag
    10501 caattactgg cgtgattccc aacatcaaaa tattgctcaa tcccgtatga atctgacgat
    10561 aggaagtatg catgctaaac tcaatcaagg cataacgact atcattaagg gtaggaattt
    10621 cttttttttc tagcttttct agagcatcca gagtatagta tatctctgcg ccataagcaa
    10681 tgactaaatc atctgctact tcttttgcaa tttcccgaac ctgaagaaag ttttctgcta
    10741 tcttctcttc cggagtttca aacatgccct tgcgacggtg agaggtagag acaatggttc
    10801 tcaccccctg cctgtaggat tctgccaaga gagccttgct ttcctctctt gacttgggac
    10861 catcatctac atcaaagacg atgtgcgaat ggatgtctat cattttatct accctccatc
    10921 acatcctgta tagctgcttt aactacagtt aaactactat catctatttc catcacatag
    10981 aggttactgt ctggcattgc ataagaagga agatccatcc gacctgtccc ttttaaatct
    11041 tgagaattta ctttataatt ccctccactt tccaactgag tattcactaa atctatcata
    11101 gtctcaatcg gtatatttgt ttgaagagaa tcctgcaatc cttgaagaat actactatag
    11161 tttttcaaaa cctctgtaga agttaacttc tgaataattg ctacaatgac cttttgttgg
    11221 ttgcgaccac ggtcacggtc tccatcggtt agtgagtagc gttcacgtac aaaacctaaa
    11281 gcttgctcag agtctagatg gacattccct actgggaaat ggaacttccc atgtagagct
    11341 gaaaactctt gatcattatg aacatctacc cctcccaata agtcaatcat tttcaagaaa
    11401 gaagtgaagt tcaatcgcac atagtaattg atatccactc catagagatt ttctaaggtg
    11461 tgaatggacg aatcaactcc ataaatgccc gcatgggtta atttatcttt ttgattattt
    11521 ccactatctg cgattggtac ataggcatca cgtggcgttg tggtcaagag gattttcttg
    11581 gtatctcgat tgacagtcat taggatattg acatctgatc gcgacaccga actaatagga
    11641 ccataggtgt caattccact aacatagata ttgaaagact gattcttaga cgtcttagga
    11701 gcctctactt ttttagtgaa tcccttggta taaatctttt ttatcttcga tgcgtagtct
    11761 ggatactctg actcgatgat attttcaaag acactattta ggacaatggc cttagtctcc
    11821 cctgcaatca aactcttgta agctgccaag taagacgaac tctggttgac cgtcaaatcg
    11881 gtattctgac ttgacttgat atcagctagt agtttttgaa tattttcatt atcagtccca
    11941 gtcggtgctg tcacactcgt cagttgcgta acattttcga tcttactatc tgctaaaaca
    12001 gcgacactga ttgaatattc tgagtaatta gaagtcgcat ttaaacgatt ggtcagtcca
    12061 acaaactgct gtactgcaaa gagcgacact gagctgacaa ggatagagaa cagcaacaga
    12121 aaaatagtaa acttttcagc ttttttatag ataatcaaga gtagccctac caaggcaact
    12181 agtaggacta acgcagttac cactaggtta agatatctaa aagcaaggat attgtactta
    12241 aagattaaga acaataaaaa acaaattaac aataaataaa tagtcagcaa aacgatatta
    12301 acacttcgct tcactttctg tgaacgtgat tttttaaaac gtctactcat gattaacacc
    12361 tataccttga acatcataca attatatcac ttttttacgg taatgtctac acctttattt
    12421 tcactatctg catctttaag tatcttagta gacttcccgc gaaccaaaa
//
LOCUS       DAVBCN010000055        12400 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVBCN010000055 DAVBCN010000000
VERSION     DAVBCN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12400
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.055"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            298..558
                     /locus_tag="VQ497_001504"
     CDS             298..558
                     /locus_tag="VQ497_001504"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2517800.1"
                     /translation="MSGGETKRVALAKIILKNPPLIFADELTASLDSTTSQGIMKLLL
                     GMKNKNRLIIIATHNPDIWNQADEVINLNQLLQSIYIFYILK"
     gene            complement(662..1132)
                     /locus_tag="VQ497_001505"
     CDS             complement(662..1132)
                     /locus_tag="VQ497_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2517801.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQTLLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(1149..3422)
                     /locus_tag="VQ497_001506"
     CDS             complement(1149..3422)
                     /locus_tag="VQ497_001506"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HET2517802.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLASDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            3769..6870
                     /locus_tag="VQ497_001507"
     CDS             3769..6870
                     /locus_tag="VQ497_001507"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET2517803.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQGVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRLAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEVREGAFYYIKGKVQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSDELEAKLNEIVMKT
                     IYR"
     gene            6952..7959
                     /gene="pfkA"
                     /locus_tag="VQ497_001508"
     CDS             6952..7959
                     /gene="pfkA"
                     /locus_tag="VQ497_001508"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET2517804.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            8016..9521
                     /gene="pyk"
                     /locus_tag="VQ497_001509"
     CDS             8016..9521
                     /gene="pyk"
                     /locus_tag="VQ497_001509"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET2517805.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(10253..10478)
                     /locus_tag="VQ497_001510"
                     /pseudo
     CDS             complement(10253..10478)
                     /locus_tag="VQ497_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            11326..12198
                     /locus_tag="VQ497_001511"
     CDS             11326..12198
                     /locus_tag="VQ497_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HET2517806.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
BASE COUNT         3786 a         2152 c         2648 g         3814 t
ORIGIN      
        1 ttgttttgtc gcttttcatt ataggtcata tgggactttt tttctacaat aaaataggct
       61 ccataatatc tatagtggat ttacccacta caaatattat agaacccttt tttattgaca
      121 gtatcaggat atctttttca aatttttggt ttgttagata atgaaaatgt ttctatcaat
      181 ctagatttag gattaataaa tcgtaaatgt aattataaag aaaaagataa gttaaaaaaa
      241 gatgtattag caagagttgg tctaactcat attcatctat aacaaaagat atatgaattg
      301 tctggcggtg aaacaaaacg tgttgcttta gcgaaaatca ttcttaaaaa tccaccatta
      361 atattcgcgg atgagttgac tgcatcactc gattcgacga cttcacaagg aattatgaaa
      421 ttgttattag gtatgaaaaa taaaaatcgt ctaatcatca ttgcaactca taatcccgat
      481 atttggaatc aagcagatga agttataaat ttaaatcaat tattacagtc aatatatatc
      541 ttctatattt taaagtaatt ttatctgaat ccgttaagag actattgtcc caggctcttt
      601 ctgatgttaa agactttcta ccaggttttt taaaagcata attgttagtt gtagtcattt
      661 attattcttc aaagaaaaat ggtggggcga attttttcag ttcttcaaag cacttttgag
      721 cagtatctgc atcttcacag atgataagac agacatcatt accacaaagg gtagcgatag
      781 cgtcagggaa gctcaaagta tcaatgatag aaccaaagga ttgagccagt ccaggaaggg
      841 tttttagtag gacttggtgt tgaactgggc gcatccagac aagggcgtct tccatgtaga
      901 gttcgagacg tttttcccat tttgagatgg aaccattgtt aagaacataa taagcgctat
      961 cttcttcgcg gacttttgat aggttcatat ttttgatgtc gcgtgagagg gttgcctgag
     1021 ttacttgaat gtcgttctca gcaagaaggg tttgcaactc agcctgtgta tgaatcttgt
     1081 tttttgtgat aagagcgcgt ataagttggt ggcggtgttc tgatttattc ataataatgt
     1141 aactcctttt agcaaggtaa ggtaagcatg gactgagcga ggtcgacagt caagtggtag
     1201 tctgtattgt cacggatggt gatttcaaag tcagtagtat agaggactaa acggagagtg
     1261 tctccttctt ttagcttgta aatagttggc tgcagttcaa attgaacgtc catccattca
     1321 tctgcagtaa tatcctctac taacagtaaa tcatttctat tttgtaaatt aaggtaacct
     1381 tttgtcacga ctctttgtgc ctctggtcta aatggcaatt cacagagatt ttccaacatg
     1441 tgatagcgac cgttgtcaat ggttctagca cttaaaatag ctggataagg ttgtaggtat
     1501 ttcttttgcc caaattctag cagttgggca gataagagcc ccttgtttgt actggatttg
     1561 atacgaagat tgagctgagc gcgaccgttt aggtggagat ctttagtcac aggaaggtta
     1621 atagtaatct gattagcttt cccttgatag agctctgtat tgaaggtttg gtatgtctta
     1681 ccatagcgct caaaatcctt atctgggtac tggttttgaa tagcttgctc ttcttgacca
     1741 agtgagaagg tttcacagtt ttcttgccca ccgaagttat caagtgataa ccaagtctgt
     1801 ggagctgtat tgtcctgcca gataacagta ggaagttgaa agtctgtttc ctgtcctagt
     1861 aatttcttgg tcaataaggc atttatggac tcacggaagt caattgattg ccaattgttc
     1921 atgtaaacat gggcaccatt atggaaaaag agatgcttgt gtatatgagt aggaagagca
     1981 tggaacatct ggtaaacatg aagtggtttg acattccaat cctgagaacc atgagtaaag
     2041 acaacctctg cctttacttt atgggcattg agcagataat tgcggtcatg ccaaaactga
     2101 ttgtagtccc cagtttttcg gtctagttga gctttcactt tttctaagtc agcttggtga
     2161 gcttcattgc cacggatata gtcgctagcc aagagattac gagaataggt taactcagca
     2221 agggagtcaa agtcctcacc tggataacca cctgggctag tcaccagacc gttttcacgg
     2281 tagtagttgt accatgatga aattcctgcc tcggcaatga taacttctaa accatcgact
     2341 cctgtagtcg caagaccatt ggacatggta cctagatagg aaagtcccgt tgtagcaact
     2401 tttccgtttg accaatcagc cttgacttga cgctggcgcg tgtgatcagt aaaggcacgg
     2461 caacggccgt taagccaatc gatgacattt ttataagcct cgatttgctg gtagtctcca
     2521 ttagtcatga aacctgtcga gtctttggta ccaacacctg agacatagag attggcaaag
     2581 cctctcggaa ggaagtagtc gtttagtgta tagctagagt tgatgtgagt tagcttttcc
     2641 tcagcctctg ctataagctc agctttacct tggggttgga cgagatttag ttgaggtttc
     2701 tctagctcaa tcttgtgagg aagtttaacc tcaagctcgc cctccatctt gtagagagcc
     2761 ttgtcactag ccttgtcatt ggttccctga tgataagggc tggctgtcat gatggcaggg
     2821 atttttccat caaaacgagg gcgaataatg ctaaccttta ctaggtctga tagccctttt
     2881 tggtcagtat cgacacgaga ctcaacgtaa acgacttcac gaatgacatc ctggttagaa
     2941 aaagtagcca aactcttgcc gttaaagtag tggtagtcat tatcctccgg aataagacca
     3001 tcactaacaa gttggtcgat aagagtattt ccttttttgg tgcgagtatt gagtaactga
     3061 tagagatttt caatcaagtc accatatata atgggaaatc cagtttcttt acgaaaaacg
     3121 tcactatctt cgaagtcaac caaataagaa aagcctaaaa gttgaaaagc aacagtataa
     3181 aaaatatctg ctgtcagttc atcttctgat tgaaaaaatg tcagcaggtc tgttttttta
     3241 tcagctgcta ggatagaaag tgggtagttg gtgtcttgat aagtgaaaaa gaaacgacgt
     3301 aaaaaggttt caagtgagtc tttgtgattg gctgtatttt gtaaatcaaa gccacatttt
     3361 tttagttcag ataagacatt ttcttttgga aaattgatat aactatattg attaaaacgc
     3421 atagaacctc catatagaat gacagttaag gttattatat caaaaaaaaa gcagaaaggg
     3481 aattgttaac ttcaaaagga aataatccaa taaaaatgaa taaagtacta aattcaatat
     3541 agagaacaga gtaacaataa gaataaatag atagggtata aaagttctag gagatttata
     3601 ttatatgctt tctattttta tatacaatat agtataaata taaaaatgat gacaaaaata
     3661 caaatgaata gaaaataaat tagtaagctg atgaaatttt tctcaagaga agccatttat
     3721 aggtgaaaat ggtataatat agtgagaagg atagaggaga agtgtaaatt gatcgcacaa
     3781 ctagatacaa aaacagtcta tagttttatg gaaagcgtca tttcgatcga aaagtatgtg
     3841 agagcagcta aagaatacgg ctacactcat ttggctatga tggatattga caatctttat
     3901 ggcgctttcg actttctaga gattacaaaa aaatacggca ttcatccttt gctagggctt
     3961 gaaatgacag tgtttgtaga tgatcaggga gtgaatttgc gctttttagc tctatctagt
     4021 gtgggctatc agcagttgat gaagctttcg acagccaaga tgcaggggga gaaaacttgg
     4081 tcagtcctgt cccagtacct ggaggatatc gcggtcattg tgccttattt tgatagagtt
     4141 gagtcgttag aactaggctg tgattactat ataggggttt atccagaaac actagcaagc
     4201 gaatttcatc atcctatctt acctctttat cgggtcaacg cttttgaaag cagggataga
     4261 gaagttcttc aagttttaac agcgattaaa gaaaatctac cgctcagaga agttcccttg
     4321 cgttcgagac aagatgtctt tatatcagca agttctttag agaaactatt ccaagagcgt
     4381 tttccgcaag ctttggacaa tttagaaaag cttatttcag gcatttctta cgacttggat
     4441 actagtctga aactgcctcg ttttaatcca gctagaccag cagtagagga gttgagagag
     4501 cgtgctgaac tggggcttgt tcagaagggg ttgactagta aagaatatca agatagacta
     4561 gaccaagaat tgtctgttat tcatgatatg ggctttgatg attatttctt ggttgtttgg
     4621 gatttgttgc gttttggacg atcgaatggc tattatatgg gaatgggaag gggttctgca
     4681 gtaggcagtt tggtttctta tgccttagac atcacgggga ttgacccagt agagaaaaat
     4741 ctgatttttg aacgctttct taatcgtgaa cgctatacca tgcctgatat tgatattgat
     4801 atcccagata tttatcgtcc agattttatc agatatgttg gtaataaata tggtagtaaa
     4861 catgcggcac aaatcgttac tttttcaacc tttggagcca agcaagctct tcgagatgtc
     4921 ttgaaacgct ttggtgtgcc agagtatgaa ttatctgcaa ttactaagaa aatcagtttt
     4981 cgtgacaatc ttaagtcggc ctatgagggc aatctccagt ttcgtcagca aatcaatagt
     5041 aagttagaat accaaaaagc ttttgagatt gcttgcaaga tagagggcta tccaaggcaa
     5101 acctctgtcc atgcggctgg tgttgtaatt agtgaccaag atttaaccaa ctacattcct
     5161 ctaaagtatg gtgatgaaat tccactgact cagtatgatg ctcatggagt tgaggctagc
     5221 ggacttttga agatggactt tctgggacta cgaaatttga cctttgtcca gaagatgcaa
     5281 gagttgcttg ctgaaacaga aggtattcat ctgaaaattg aagaaatcga tttagaagac
     5341 aaagaaacat tagctttatt tgcctctggt aatacaaaag gtatctttca atttgagcaa
     5401 ccaggtgcca ttcgtctgct taagcgtgtg caaccagtct gttttgaaga tgtcgtcgcg
     5461 actacttctc taaatcgacc gggtgctagt gactatatca ataattttgt ggcaagaaag
     5521 catgggcagg aagaagtgac tgttctggat ccagtactgg aggatatttt ggctccaacc
     5581 tacggcataa tgctctatca ggagcaggtt atgcaggttg cccagcgact tgccggattt
     5641 agtcttggga aagccgatat tttgcgtcgg gctatgggga aaaaggatgc ctctgccatg
     5701 catgagatga gggcttcctt tattcaaggt tcattagaag ctggtcatac tgtggaaaaa
     5761 gcagagcagg tctttgatgt tatggagaag tttgcaggtt atggttttaa caggtcacac
     5821 gcctatgcct actcagcctt ggccttccag ttggcttatt tcaaaacgca ttatccagcc
     5881 attttttatc aggtcatgtt aaattcttcc aacagtgatt acttaataga tgcacttgaa
     5941 gcaggttttg aagtagcctc tctatccatc aacaccattc cctatcacga taaaattgcc
     6001 aacaaggcca tctatctagg tttgaaatcc attaaaggag tcagtaatga tttagctctc
     6061 tggattattg aaaatagacc ttattctaac attgaagatt ttatagctaa attacctgag
     6121 aattatctga aacttcctct gctagaacct ttggtaaaag ttggtctttt cgattcattt
     6181 gaaaaaaatc gtcaaaaagt atttaataac ttagctaatc tatttgaatt tgtgaaagag
     6241 ttgggaagtt tgtttggaga tgctatttat agttggcagg aatcggaaga ttggacggaa
     6301 caagaaaaat tttatatgga acaagagctt ttagggatag gtgtcagcaa acatccacta
     6361 caagctattg caagtaaggc tatttacccg attaccccaa tcggaaattt gtcagaaaat
     6421 agctatgcta ttatcttggt tgaagttcag aaaataaaag tgattcgtac caaaaagggt
     6481 gaaaatatgg ccttcttaca ggcagatgat agtaagaaaa aattggatgt tacactcttc
     6541 tcagatttat atcgacaggt tggtcaggaa gtaagagaag gagcattcta ctatatcaaa
     6601 gggaaagtcc aatcacgtga cggtcgtctg caaatgattg cacaagaaat aagagaagca
     6661 gttgctgaac gcttttggat acaggtgaaa aatcatgaat cggatcaaga aatttcacgt
     6721 attttagaac aatttaaagg cccaatccca gtcatcatcc ggtatgaaga ggaacagaaa
     6781 accatcgttt ctccccatca ttttgtagct aaatctgatg aattagaggc gaaattgaat
     6841 gaaatcgtta tgaaaacgat ttatcgctaa aaatacggaa aatagaagaa ttttaaaatc
     6901 aaatgtgata taatcaagaa gaatgttaaa agaaaaagga gcataaccaa tatgaaacgt
     6961 attgctgttt tgactagtgg tggagacgcc cctggtatga acgctgccat ccgtgcagtt
     7021 gttcgtcaag caatttcaga aggaatggaa gtgtttggta tctatgacgg atatgctggt
     7081 atggttgccg gtgaaattca tcccctagat gcagcttcag taggggacat catttctcgt
     7141 ggtggtactt tccttcactc agctcgttac ccagagttcg ctcaacttga agggcaactt
     7201 aaagggattg agcaattgaa aaaacacgga attgaaggtg tagttgttat cggtggtgac
     7261 ggatcttacc acggcgctat gcgtttgact gaacatggct tcccagctat tggtcttcca
     7321 ggtacaatcg ataacgatat cgttggtact gactttacaa tcggttttga cacagcggtt
     7381 actactgcca tggacgctat cgataagatt cgtgatacat catcaagtca ccgtcgtact
     7441 tttgtaatcg aagttatggg acgtaacgct ggtgatatcg ctctttgggc tggtattgca
     7501 actggtgctg atgaaatcat catccctgaa gcaggcttca agatggaaga tatcgtagca
     7561 agcatcaaag ctggttatga atgtggtaaa aaacacaata tcatcgtctt agctgaaggt
     7621 gtgatgtcag cggctgaatt tggtcaaaaa cttaaagaag ctggagatac aagcgacctt
     7681 cgtgtaacag aacttggaca tattcaacgt ggtggttctc caactgcgcg tgaccgtgtt
     7741 ttggcgtcac gtatgggtgc acatgctgtt aaacttctta aagaaggtat cggtggtgtt
     7801 gcggttggta ttcgtaacga aaaaatggtt gaaaatccaa ttcttggtac tgcagaagaa
     7861 ggggcattgt ttagccttac tgcagaaggt aagattgtgg ttaacaaccc gcacaaagcc
     7921 gatattgagc tatctagctt gaataagagc ttgtcataat ttacaattta gttaataaga
     7981 ccaaaaaggt catatataaa ggagtcacaa aaatcatgaa caaacgtgta aagatcgttg
     8041 caactttggg tcctgcggta gaaatccgtg gtggtaaaaa attcggtgag gacggatact
     8101 ggggtgaaaa acttgatgtt gaagcttcag ctaaaaacat tgctaaattg attgaagctg
     8161 gtgctaacac attccgattc aacttctcac acggcgacca ccaagaacaa ggtgagcgta
     8221 tggcaactgt taaacttgcg gaaaaaattg caggtaaaaa agttggtttc cttcttgata
     8281 caaaaggacc tgaaatccgt acagaattgt tcgaaggtga agctaaagaa tattcataca
     8341 aaactggtga aaaaattcgt gttgcaacta aacaaggaat caaatcaact cgtgaagtga
     8401 ttgcgttgaa cgttgctggt gctcttgata tctatgatga tgttgaagtt ggtcgtcaag
     8461 ttttggttga cgatggtaaa cttggtcttc gtgtggttgc taaagatgat gcaactcgtg
     8521 aatttgaagt tgaagttgaa aacgatggta tcatcgctaa acaaaaaggt gtgaacatcc
     8581 ctaacactaa aattcctttc ccagctcttg ctgaacgcga taacgacgat atccgtttcg
     8641 gtcttgaaca aggtatcaac ttcatcgcaa tttcattcgt acgtactgca aaagatgtga
     8701 acgaagttcg tgcaatctgt gaagaaactg gaaacggaca tgttcaatta ttcgctaaaa
     8761 tcgaaaacca acaaggtatc gataacttag acgaaatcat cgaagcagct gatggtatta
     8821 tgattgctcg tggtgatatg ggtatcgaag taccgttcga aatggttcca gtttatcaaa
     8881 aaatgattat caagaaagtc aatgctgcag gtaaagttgt tatcactgca acaaacatgc
     8941 ttgaaacaat gactgaaaaa ccacgtgcaa ctcgttcaga agtatcagac gtattcaacg
     9001 ctgttatcga cggaactgac gctacaatgc tgtcaggcga gtctgcaaac ggtaaatacc
     9061 cactcgagtc agtaactaca atggctacaa tcgacaagaa tgctcaagct cttcttaatg
     9121 aatacggacg tcttgattca gattcatttg agcgtaactc taagacagaa gtaatggctt
     9181 ctgctgttaa agatgctact agctcaatgg atatcaaatt ggttgtaact cttactaaga
     9241 caggtcatac tgcacgtttg atttctaaat accgtccaaa tgctgacatc ttagcattga
     9301 catttgacga attgacagaa cgtggcttga tgttgaactg gggtgttatc ccaatgttga
     9361 cagatgctcc atcttcaact gacgatatgt tcgaaatcgc tgaacgtaaa gcggtagaag
     9421 caggtctcgt tgagtcaggc gatgatatcg ttatcgttgc tggtgtgcca gtaggagaag
     9481 ctgttcgcac aaacacaatg cgtatccgca cagtacgtta agaaaaatat aaaaacctat
     9541 catatccagc tttagagctt gtgtgatagg ctttttgtat agagggtaag aaataggcaa
     9601 aactttcata atggattgat actcttcgaa aatcaaattc aaaccacgtc agcgtcgcct
     9661 taccgtatat atgttactga cttcgtcagt tctatctaca acctcaaagc agtgctttga
     9721 gcaacctgcg gctagcttcc tagtttgctc tttgattttc attgagtatg aaataagata
     9781 tgcacaaatt gattaggaaa ttcaaatgaa tttctacaaa tgttttagca atcgtaatgt
     9841 acttgtctag attcgatctg atatattttc gatttaatga tatggtattt aaaacctcca
     9901 aagtagcttg ctccattctt ttacttacgt gagtgtagat gttatttact gttttagcgt
     9961 ttttgtgttc cactctaacc attatagcat tcttctcagc tagtgtacta aggagtgtgt
    10021 gcctgaaaat atgggaacta aggggctggt ttatcggttt ctctagttta gtatttgcct
    10081 tttgcaaagt gatgttaaat gcctttctct aaatttacat atcactattg tttaacaaaa
    10141 tctaatctac tttaggtcac ttattctttt tttgaaatgt agaatgaact ttttcaaagt
    10201 ttttcgaatc ttttaaaatc tgtttgtttt atatcgccat tctccccctt ttttaattct
    10261 ccctatatag cctgacaact ttcccgatgg tacgaatatg gttgctttcg tctaggtgga
    10321 tgtcggggta tttgggattg agttttttta aggtagcctt ggcggagttt cttgacatag
    10381 ttagcgccgt ctacttggaa gatgccgatg gtattatagt caatctgtgg ggtattcttg
    10441 ataaataggt agtcgctgtt tcttatcttt ggctccatgg acttgctgac gacataagcg
    10501 attgggtcgt agtcgtctgg gataatggaa actccataac taaatcgtcg tcctgcatcg
    10561 agcggctacc tgcagagata aactacctaa cacgagagta agtagtctgt ctgtagtcgt
    10621 ccagtctgat gatttttacg atatttcgtt tttctgctca tacagttgcc tctcggcata
    10681 ggtcagaact ttaccttgtc tgggtggtgc ccgttggtcg tagatagatt ggatatcgct
    10741 aggagaatcc ttttgaactg gaggaaagag ggtatcaatc aagctactga atactttagc
    10801 taagtcaaat atagtatttt tcttagtaga cctaaccctt ttttcataat ttccaatggt
    10861 gtttttactt ataccaatct tagtacccaa ttcttattga gtccaaccat tactagtcta
    10921 tattgtttta tagttgattg agtttggaat agtacgctgt agctgctaaa acatttctag
    10981 gaattaattt gactttccta atagagttgt tcatatctta tttcaatcta ctatgttttt
    11041 cacctctaac aatcgcaatc tcttctttat ctatgaatga aatcgctttc tatttttgta
    11101 agtaaagcat cacacgaaat ccacgaaaat gaaaaccttt gttgtgtttt cgtaaaaaat
    11161 ttgttgacag agcacgaaac gcctactata attaaatcaa gcttaaggaa ataacaaaaa
    11221 aacaaatcgg agggaaagac gatgaacaca ttaaatgaga aagtaatcaa tatctgtaaa
    11281 gcagtagtta aagaaacttt aatccaagac atttaggaga aagaaatgaa gaaatcaaga
    11341 aaattagcca cattaggaat ctgttcagcc ttgtttttag gcttggctgc ttgtcaacaa
    11401 caacatgcta cttctgaggg gacgaatcaa aggcaaagca gttcagcgaa agttccatgg
    11461 aaagcttcat acaccaacct aaacaaccag gtaagtacag aagaggtcaa atctctctta
    11521 tcagctcact tggatccaaa tagtgttgat gcatttttta atctcgttaa tgactataat
    11581 accattgtcg gctcaactgg cttatcagga gatttcactt cctttactca caccgaatac
    11641 gatgttgaga aaatcagtca tctctggaat caaaagaagg gcgatttcgt tgggaccaac
    11701 tgccgtatca atagttattg tcttttgaaa aattcagtca ccattccaaa gcttgaaaag
    11761 aatgaccaat tgcttttcct agataacgat gcgattgata aaggaaaggt ctttgattca
    11821 caagataagg aagagtttga tattctattt tcgagagttc caactgaggc aactacagat
    11881 gtcaaggttc acgctgaaaa gatggaaaca ttcttctcac aatttcaatt caatgaaaaa
    11941 gctcgaatgc tgtctgtagt cttgcacgac aatctggatg gcgagtatct gtttgtaggc
    12001 catgttgggg tcttagtacc tgctgatgac ggtttcttat ttgtagagaa attgactttc
    12061 gaagagccct accaagcgat taaatttgct agtaaggaag attgctacaa gtatttgggc
    12121 accaagtatg cggattatac aggcgaggga ctggctaagc cttttatcat ggataatgat
    12181 aagtgggtta aactttaata gtgttatgat atgacagtaa tcttaggatt tttatttcta
    12241 tttttgatga cttggtgggt tatttcaatc gttaatagcc agaaaccacg caaaaatgaa
    12301 acatttattg gctatatcca aagatacgat attgatggga atggctatca atcattaccg
    12361 tgtcctcaga actgagagga gttcttgaaa ccgtaaaacc
//
LOCUS       DAVBCN010000056        12257 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVBCN010000056 DAVBCN010000000
VERSION     DAVBCN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12257
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.056"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(259..2517)
                     /locus_tag="VQ497_001512"
     CDS             complement(259..2517)
                     /locus_tag="VQ497_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2517807.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2746..2976)
                     /locus_tag="VQ497_001513"
     CDS             complement(2746..2976)
                     /locus_tag="VQ497_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HET2517808.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3166..3849
                     /locus_tag="VQ497_001514"
     CDS             3166..3849
                     /locus_tag="VQ497_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2517809.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSEIVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPVLGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3849..4583
                     /locus_tag="VQ497_001515"
     CDS             3849..4583
                     /locus_tag="VQ497_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2517810.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4737..5594
                     /locus_tag="VQ497_001516"
     CDS             4737..5594
                     /locus_tag="VQ497_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HET2517811.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5736..6608
                     /locus_tag="VQ497_001517"
     CDS             5736..6608
                     /locus_tag="VQ497_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET2517812.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLMTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVILLGPGL
                     GDNAFGEDLVKQVFAGLKQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            6890..7573
                     /gene="rpiA"
                     /locus_tag="VQ497_001518"
     CDS             6890..7573
                     /gene="rpiA"
                     /locus_tag="VQ497_001518"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HET2517813.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7587..8798
                     /locus_tag="VQ497_001519"
     CDS             7587..8798
                     /locus_tag="VQ497_001519"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HET2517814.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIRESNKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8800..9342
                     /locus_tag="VQ497_001520"
     CDS             8800..9342
                     /locus_tag="VQ497_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET2517815.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            9358..10167
                     /locus_tag="VQ497_001521"
     CDS             9358..10167
                     /locus_tag="VQ497_001521"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET2517816.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10643..11623
                     /locus_tag="VQ497_001522"
                     /pseudo
     CDS             10643..11623
                     /locus_tag="VQ497_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
     gene            11648..12160
                     /locus_tag="VQ497_001523"
     CDS             11648..12160
                     /locus_tag="VQ497_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HET2517817.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPISAQPHRKITHWGTTL
                     NSKVSDDDVL"
BASE COUNT         3614 a         2238 c         2776 g         3629 t
ORIGIN      
        1 ttgttgcaat accttttacg aggctctttt gtcctgctct tgtttcaatt gactatagca
       61 gattgaaata agatgagaac aaatcgattg ggaaagtaaa attaatttct ataaatgttt
      121 tagcaattgt ttcgtactat ttcagattca gtctactata tatcataatg gacaaagggc
      181 aagctgtcaa gaaattacag ttattttagt aaaaaaagaa gcagaaaaat ttaattttct
      241 actccttttc tataggattt atttttcttt ttcagaactt ttaacttcag cttttttggc
      301 agatttaatc tgaatgtttc ccttgctagt cataactgct ttgagatctt tttcgcttgg
      361 attttcaagg tagtagtcag tgattgtgtc ctcaatatag tcttgaatag tccgacgaag
      421 tgggcgtgct cccatttttg gatcataacc taggtcaacc aacttttcct tgaccttgtc
      481 agttacatcc aaacgaatgt tgttgctaga gaggcgcttg ttaacatctg ctagcatgag
      541 ctcgacaatc tgaaggaggt tatccttgct gagagcctta aattcgataa tgccatcaaa
      601 acggttcata aactctgggc taaagaagtt accgagttca ccgaggacag aattggtacg
      661 tccttctcta gcagcaccaa atccaacgct agcttcggtc tttcctgtac ctgcatttga
      721 ggtcatgata atgatggcat ccttgaagct aacggtgcgt ccttgcccgt ctgtcaaacg
      781 accatcgtcc aagacttgaa gaaacatgtg cataacatct gggtgagctt tttccacttc
      841 atcgagaagg atgagagaat atggattgtg gcgaactttt tcagttaatt gaccagcctc
      901 atcatagcca acataacctg gaggagcgcc gaccaactta gccacactat gtttttccat
      961 gtattcactc atatcaaagc gaatcatact atcagcagaa ccaaaaagtt cgatagccag
     1021 ttgtttggaa agttctgtct taccgacacc agttggccca acgaagagga agcttccgat
     1081 tgggcggtta ggggtaccaa gtccgacacg attacggcga atagccttgg caatcttatc
     1141 gactgcatca tcttggccaa taacatgaga cttgagatct tcggctagat ggatgagttg
     1201 agattgttct ttctctttca aatcaccaac agggatattg gttttctgct cgataatgtg
     1261 ctcaatagtt ttctcgctga tgataggagt atcctggtct gtgatctttt tcttttgcat
     1321 ttccttatac ttggcaatct ggtcgcggaa gtaggccgcc ttctcaaaat cttcttctcg
     1381 tgtagcttga gacttgagat tttcagcctc aatcaagcgc tgatcaatta ctttaggatc
     1441 cacaaaattc aaggtcaagt tcatcttaga accagcttca tctaggaggt caatggcctt
     1501 gtcaggcaag aagcgatctt ggatgtagcg attggaaaga gttgcagctg cttcaatcgc
     1561 agcatctgta tattgaacgt ggtggtaatc ttcgtatttc ttttgaatcc ctttgagaat
     1621 agtgattgtt tcgtccaccg ttggttcatc gactttaaca ggctgcatac gacgctcgag
     1681 ggcagcatcc ttttcaatga tacggtattc attgagggta gtagcaccga ctagttgcag
     1741 ttctccacga gcaagggctg gcttgaggat atttcctgcg tccatattac catcactcgc
     1801 agaaccagca ccaacaattt catggatttc atcgataaag aggatgatgt cttcacgttt
     1861 gcgaatttct tccatgagtt tttgcatgcg ttcttcaaat tgtcctcgaa tccccgttcc
     1921 ttgaactaag ctaaccacat ccagacggat gacttgttta ccttggagtt tatgtggcac
     1981 atcgccatcg acaattttct gagctagacc ttcgacaacg gccgtttttc cgacaccagg
     2041 ttcaccgata aggacaggat tattcttggt tctacgattg agaatctcga tgacacggat
     2101 aatctcatcg tcgcgcccaa taacggggtc aatgtctcca cgacgggcaa tttcagttac
     2161 attaatacca aattcttcca gcaggccttt ttcttggcta ggtggcggag tttgagcaga
     2221 tccacgattt tgggaaccat aaccgccgtt tccaccgtaa cctccacctg attgggttgg
     2281 gggaatagga ggagtattgc tagaaggtct gaaattgttt agatcattga agaaatcacc
     2341 aaagggatcg aagtcacgat tgttcagatc cgtcatacct ttgaagaggc tattgttagg
     2401 atctgttttg ataatcttat agcagttttg acagaggtca atttgttttt gttttccatt
     2461 gagattggtg taaagatgaa ttgttgagtc gttaatttta cagttttgac aaagcataca
     2521 tctacctcat ttctttcttt agcctgactt gtttaaaggt caaaaatagt caaattctat
     2581 actctcatta taacaggatt ggggtgtaaa gcaagtaaaa agattgaagc tttgagaagt
     2641 tgttacaatg aaaatttttg tgaatttgga ctataaaaaa atcctatttg tgagacaaaa
     2701 aggattttac ttagacatgc agggacaatc ccagcttgtt ttagattagt agagaagttc
     2761 gtaaatttcc attgcgatta agtcaatgct atcaaacgta tacgtttcgc gatcttctac
     2821 gtcacgaagg gtaaagattg ggccgttttc ttcgtcgtgg ctgtacgcaa cttctgcaac
     2881 aacaactcct tcgcgttcaa aattacgttt taaatttccg ccatcgtttg ccattgcttc
     2941 aaggcggttg atgattctaa ccaaatgtga ttccattttg attctccttc atttcttatc
     3001 gttactattt taccataaag gaggccaact tgactagaaa aaactaagat ttctcgctaa
     3061 ttctgctagt ttaaagattt ttaaataaat tggataaaaa atttaaattt taattcaaaa
     3121 catgttataa tcatacagta ttctatttta gaaagcagtg tgactatgaa tttttctttt
     3181 ttacctaagt atttacctta ttttaactat ggggctgttg tgacgattct tatttctatc
     3241 tgtgttatct ttttgggaac tattttgggt gttgtcttgg cttttgggca acgttcaaag
     3301 tttaaaccgc ttgtttggtt ggccaacttg tacgtttgga ttttccgtgg gacaccgatg
     3361 atggttcaaa ttatgattgc ctttgctctt atgcatatca atgctccgac tattcagatt
     3421 ggaattttag gtgttgattt ttcgcgtctg attccaggga ttttgattat ctctatgaat
     3481 agtggtgctt atgtttcgga gattgttcgt gccggaatca atgcggttcc aaaaggtcag
     3541 ctagaggcgg cttattcgct agggattcgt cctaaaaatg cgatgcgtta tgtgattttg
     3601 ccacaagcag tcaaaaatat cttgccagta ttggggaacg aatttatcac cattatcaag
     3661 gacagctccc tcttatcagc tattggggtc atggagttgt ggaatggggc tacaacagtt
     3721 tctacaacaa cctatctacc tttaacacca cttttatttg cagcatttta ctacttgatt
     3781 atgacctcta ttctgacagt agccttgaaa gcttttgaaa aacatatggg acaaggagat
     3841 aagaaataat gacagaaacc ttgataaaaa ttgaaaattt acataaatct tttggaaaga
     3901 atgaagtatt gaagggcatc aacctcgaga ttaaaagagg agaagttgtc gttatcatcg
     3961 gtccttcagg gagtgggaaa tctaccttgc ttcgctctat gaatttgttg gaggaagcaa
     4021 ccaaggggaa ggttatcttt gagggagtcg atattacgga caagaagaat gacctgtttg
     4081 ccatgcgtga gaagatgggc atggttttcc aacaatttaa cctctttcct aatatgactg
     4141 tgatggaaaa tattaccttg tcacctatca agactaaagg tgaaagcagg gaagttgctg
     4201 agaagagagc ccaagagctt ttggaaaaag ttggtttgcc agataaggca gacgcttatc
     4261 cacagagtct gtcgggtggg caacaacaac ggattgccat cgcgcgtggg ttggctatgg
     4321 aaccagatgt tttgctcttt gacgaaccaa cttcagcctt ggatcctgaa atggtaggtg
     4381 aggtattggc tgttatgcaa gaccttgcca agtcagggat gactatggtt atcgtaacac
     4441 atgagatggg atttgcccgt gaggtggcag atcgtgttat ctttatggca gacggtgtgg
     4501 ttgttgaaga tgggacacct gagcagattt ttgaacaaac ccaagaacaa cggactaaag
     4561 atttcttgag taaggtttta taatctattt taaaatttca agacaagatt agtgaaaagt
     4621 tcaaccagag ctttttctta tagtttgaaa ctataggata acctaggaaa gaagtgttag
     4681 agggcactag cagtttttgg tataatggaa gatatttgaa agaaaagaga agtgatatga
     4741 cacagattat tgatgggaaa gctttagcgg ccaaattgca ggggcagttg gctgaaaaga
     4801 ctgcaaaatt aaaggaggaa acagctctag tgcctggttt ggtagtgatt ttggttgggg
     4861 ataatccagc cagccaagtc tacgttcgca acaaggagag gtcagctctt gcggctggtt
     4921 cccgtagcga agtagtgcga gttccagaga ccattactca agaggaattg ttagacctga
     4981 ttgctaaata taatcaggat ccagcttggc atgggatttt ggtccagttg ccattaccaa
     5041 aacatatcga tgaagaggcg gttttattag ccattgaccc agaaaaggat gtggatggtt
     5101 tccatcccct aaacatggga cgtctttggt ctggacatcc agtcatgatt ccctcgacac
     5161 ctgcaggaat tatggaaatg tttcatgaat atgggattga cttggaaggt aaaaatgcgg
     5221 tcgtaatcgg tcgttcaaat atcgttggaa aacctatggc tcagcttctt ttggctaaaa
     5281 atgcgacagt aaccttgacc cactcacgaa ctcataatct tgccaaggtg gctgctaaag
     5341 cagatattct tgtagtcgca atcggccgcg ccaagtttgt gactgctgac tttgtcaaac
     5401 caggtgcggt tgtcattgac gttgggatga atcgagatga aaatgggaag ctctgtggag
     5461 atgttgatta tgaggcagtt gcaccacttg ctagccatat cacgccagtc cctggaggtg
     5521 tcggtcctat gaccattacc atgctgatgg agcaaactta tcaagcagca cttcggacat
     5581 tggatagaaa ataagagaag cattcgatta atagagtgta ttttctatag ctatatctaa
     5641 aatggcagaa atgaatatta aattttagat ataagattac aaaaggaggt ctgcgtctcc
     5701 tttttattgt ataataaaag tgagaggaaa aaaggatgaa agtgattaat caaaccttat
     5761 tagaaaaagt tattattgaa cgttctcgtt caagtcataa aggagattat ggtcgtctgc
     5821 tgctgcttgg tggtacttat ccttatggag gtgccatcat catggctgct ttagcagctg
     5881 taaaaagcgg tgcaggattg atgaccgttg gaacggacag ggaaaatatc ccggctctgc
     5941 acagccattt acctgagcct atggcctttt ctcttcaaga ccagcaattg ttaaaagagc
     6001 aattggagaa ggcagaagtt atcttgctgg ggcctggttt aggagacaat gcttttggag
     6061 aagatttagt taaacaggtc tttgctggct taaaacagaa tcagattttg attgtagacg
     6121 gaggtgcctt gaccattctt gctaggacaa gtttgtcatt tccctcaagc cagcttatcc
     6181 taactcctca ccaaaaagaa tgggaaaaat tatctggaat tacgattgaa aagcaaaagg
     6241 aagatgcaac ggctagtgtt ctgacttcct tccctcaagg aacaattttg gtggagaaag
     6301 gcccagctac tcgtatttgg gaagttggcc aatctgatta ttaccagtta caggttggtg
     6361 gtccttatca ggcgactggg ggaatgggag atactctggc tgggatgatc gcaggatttg
     6421 taggccaatt ccgacaggcc agtctctacg agcgtgtggc agtagcgacc catcttcatt
     6481 cagccatagc ccaagaacta tctcaagaaa attatgtggt cttaccgacg gaaatcagca
     6541 ggtatcttcc caaaataatg aaaattattt gtcaacaaga gagggtaagc aaagataaac
     6601 ttgtttaaaa tagacagata aatctacttc cttctcttaa tttatgattc ttgaaatgcg
     6661 cctgctttaa ggctaaacta gacgtatcaa agttaagagt ttgtttttgt aactggaaag
     6721 agatgaacaa atcaaataca taaaagataa actcattttt attcgttttg taaaaataaa
     6781 atgcatattt ttaaagaaag attgatgaaa aatgtagatt tgatgttttt acttgaataa
     6841 aaagcggttt catagtaaaa taagaactga gaatgatgga ggtaaatcgg tggaaaatct
     6901 gaagaaaatg gcaggtatca aggctgctga gttcgtgagc gatggaatgg tcgttggact
     6961 tggaacaggc tcgactgcct attattttgt cgaagaaatc ggtcgtcgta tcaaggaaga
     7021 aggattgcag attacagctg taacaacttc tagtgtgacc actaaacagg cagaaggcct
     7081 taatatcccg ctcaagtcta ttgaccaagt agactttgtc gatgtgacgg ttgatggcgc
     7141 ggatgaagtg gatagtcagt ttaatggaat caaaggcggt ggtggtgccc ttctcatgga
     7201 aaaggtggtc gcaacaccat caaaagaata catttgggtg gtggatgaaa gcaagctggt
     7261 cgaaaaacta ggtgctttta aattgccagt agaagtggtt cagtatggtg cagagcaggt
     7321 ctttcgtcat tttgaacgag ctggctacaa accaagtttc cgtgaaaaag acggtcaacg
     7381 ttttgtgacg gatatgcaga attttatcat tgacctcgcc ttggatgtca ttgaaaatcc
     7441 aattgctttt ggacaagaat tggaccatgt cgttggtgtt gtggagcatg gtttattcaa
     7501 ccaaatggtg gataaggtaa tcgttgctgg acgagatgga gttcagattt caacttcaaa
     7561 aaaaggaaaa tagaaggggg cataagatgt ctaaatttaa tcgtattcat ttggtggtac
     7621 tggattctgt aggaatcggt gcagcaccag atgctaataa ctttgtcaat gcaggggttc
     7681 cagatggagc ttctgacaca ctgggacaca tttcaaaaac agttggtttg aatgtcccaa
     7741 acatggctaa aataggtctt ggaaatattc ctcgtgaaac tcctcttaag actgtagcag
     7801 ctgaaagcaa tccaactgga tatgcaacaa aattagagga agtatctctt ggtaaggata
     7861 ctatgactgg acactgggaa atcatgggac tcaacattac tgagcctttc gatactttct
     7921 ggaacggatt cccagaagaa atcctgacaa aaatcgaaga attctcagga cgcaaggtta
     7981 ttcgtgaatc caacaaacct tattcaggaa cggctgttat cgatgatttt ggaccacgtc
     8041 agatggaaac tggagagttg attatctata cttcagctga ccctgttttg cagattgctg
     8101 cccacgaaga cattattcct ttggatgaat tgtaccgtat ctgtgaatac gctcgttcga
     8161 ttacccttga gcgtcctgcc cttcttggtc gcatcattgc tcgcccttat gtaggtgaac
     8221 caggtaactt cactcgtacg gcaaaccgtc gtgacttggc tgtatctcca ttttccccaa
     8281 ctgttttgga taaattgaat gaggctggta tcgatactta tgctgtgggt aaaatcaacg
     8341 atatctttaa cggtgctggt atcaaccatg acatgggtca caacaagtca aatagtcatg
     8401 gaattgatac actattgaag actatgggac ttgctgagtt tgaaaaagga ttctcattca
     8461 caaacctagt tgactttgat gccctttacg gccatcgtcg taatgctcac ggttaccgtg
     8521 attgcttgca tgagtttgat gaacgcttac ctgaaattat cgcagctatg agagagaatg
     8581 accttctctt gattactgcg gaccatggaa atgacccaac gtatgcagga acggatcaca
     8641 ctcgggaata tattccattg ttggcctata gccctgcctt taaaggaaat ggtctcattc
     8701 cagtaggaca ttttgcagat atttcagcga ctgttgccga taactttggt gtggaaactg
     8761 ctatgattgg ggaaagtttc ttagataaat tggtataaga tgacgcgcta tgctttgctg
     8821 gtgagaggta tcaatgttgg tggtaagaat aaggtcgtca tggcggagct tcgtcaagaa
     8881 ttgacaaact tgggactgga aaaggttgag agctacatca atagtggcaa tattttcttt
     8941 acttcgatag attccaaagc ccaattggtt gaaaagctag agactttctt tgcagtccat
     9001 tatccattta ttcagagctt ttctttactg agtctagagg actttgaggc ggaacttgaa
     9061 aatctaccag cttggtggag cagagacttg gcacgaaaag attttctctt ttacactgag
     9121 ggtttggatg tggaccaagt catcgcgaca gttgaaagtt tagagctgaa agatgaagtg
     9181 ctttattttg gaaaacttgg gattttctgg gggaaatttt ctgaagaatc ctattctaag
     9241 actgcctatc ataagtactt gctgaaggtg cctttctacc gccacattac tattcgtaat
     9301 gctaaaacct ttgacaaaat tggtcaaatg ctaaaaaaat aataaaggag acacacaatg
     9361 acatttttaa acaaaatcca tgaaactgct actttcctga aagaaaaggg aattgcagcc
     9421 cctgagttcg gtctaatcct tggatcagga cttggagaat tggcagaaga aatcgaaaat
     9481 ccagttgtag tagactatgc tgagattcca aactgggggc gttcaacagt agtcggtcat
     9541 gctggtaaat tggtatatgg tgaactggca ggtcgcaagg tcttggctct tcaagggcgt
     9601 ttccatttct atgaagggaa tcctctggaa gtggtgactt tcccagttcg tgtgatgaaa
     9661 gttcttggat gtgaaggtgt tattgtaacc aatgcagctg gcggtatcgg atttggtcct
     9721 ggtaccttga tggctatctc agaccatatc aacatgacgg ggcaaaatcc attgatgggt
     9781 gaaaacttgg atgactttgg cccacgtttc ccagatatgt ctagggccta cacaccagaa
     9841 taccgtgcca ctgcccatga agtggctaaa aaacttaata tcaagcttga tgaaggtgtc
     9901 tatatcggag ttactggtcc gacttatgaa acaccagcag aaattcgttc ctataagaca
     9961 ctgggagcag atgcagttgg tatgtctacg gttcctgaag ttatcgtggc agcccactct
    10021 ggcttgaaag ttctgggaat ttcatgtatc actaactttg cggccggttt ccaagaagaa
    10081 ctcaatcacg aagaagttgt agaagtgact gaacgtgtta aaggtgattt caaaggcttg
    10141 cttaaagcga ttcttgctga attgtaagaa aaaagattta aaagggggag tgcctctgtt
    10201 ttttcaggat tgactgccta tccggattaa agaagaaaca gaggaatact atgagcttct
    10261 tcctgctctt ataactgaaa gaagcggaag aataggtatg tctgatctga tagccagcat
    10321 tgtgaaagac aagattctag gatactagca ttagcttcct agccaagcag actagtatga
    10381 taaggagaga tgagaatgaa ttgactttct gaatttctca gtcttatcat atatagcaca
    10441 atgagatttc gcttgagtct gcttgtaaat aaacgaaaag aaagataaga aataatgaaa
    10501 attggtcaac gaattatgcg ctttggcata aaaaattaag tatcggagtt gtatctgttg
    10561 tagtcggctt tgatttctag ctccagctgg aatttcagcc aatgaagtaa agcaagatgt
    10621 aacatctgaa gtggtaatag gtgtgctaga ttctaaggag gaattgaaag agtcagaaaa
    10681 tgatgctcca aaactagaaa ctcctcttag agaggagcca agactagctc ctcaaacgct
    10741 tccggaagca agtgaagttc ttgaaaacaa aagggaagag tcaaaagtag agataacata
    10801 accagctcaa gcggatgata tccgcaaggt tgttggggaa ttagccaagg atataagtat
    10861 tactaagttg tatatgacag gtcattctct tggatgttac ctagctcaga ttgcagcggt
    10921 tgaagcttac caaaaatatc ctgattttta taaccatgta ttgaggaaag tgacaacttt
    10981 cagtgctcct aaagtgatta cttccagaac tgtttggaat gctaagaatg gtttctggga
    11041 tgttggtttg gaaagtcgta aattagctgt tagtggaaaa attaagcatt atgtggttga
    11101 taatgacaat gttgtgactc ccttgattca taataatcgt gatattgtta catttacagg
    11161 taattcacgc tttaaacacc gttctcgtgg ctattttgaa agtccaatga atgatattcc
    11221 taactttaat attggtaaac aagctacctt ggataaacat ggttatcgtg atccgaaatt
    11281 ggataaagtg cgattcttta agaaacaggc tctgcctcga tcttctagtc aaccaagcgc
    11341 tgaaccaatg gaaaatattg ccttaggaaa acaggttact caaagttcga cagctttcgg
    11401 aggagatgct agaagagctg tggatggcaa agtcgatggt aactatggtc acaattctgt
    11461 cactcataca aacttccaat ctaagccttg gtggcaagta gatttggcta aagaagaaac
    11521 cattcgccaa atcaatattt acaaccgaac agacactgcc caggatagat tggcaaactt
    11581 tgatgtcatt cttttagaca gttctggtaa agaaattgag tgaaaacgta taatatctcc
    11641 taaagatgtg tcagcacaaa ttacgattaa ccataaaaaa gcgcgctatg ttcggattga
    11701 gctagaaggc tataatgccc tcagtcttgc agaagttgaa gttttccgct ttatagctac
    11761 gaatgctgaa acggcgacac aagtttctaa gccagttcaa ccaatcagtc agactcctgt
    11821 gaaagataaa acattgacaa ttcaacacag tggagcttac attgcccgct actccataac
    11881 ttgggaagaa gttccagtag ataaagatgg aaaccaagtt gttcgtagtc attcttggga
    11941 aggaaacggt cgcaaccaga ctgcgggttt tgtcctcaac ctcccaatca aagaaaatat
    12001 gagaaatctg cgagttaaga ttgagaaaaa gacgggccta ctatggaata gatggcaaac
    12061 aatctatgaa aacagaccaa tttcagctca accccaccgt aaaattaccc attggggtac
    12121 gacattgaat tccaaggtga gtgacgatga tgtcttgtaa tctgatggta caatgacagt
    12181 tagtttgtct agtttataag aaagtactac ctgagcttga atagaactca ggtagctctc
    12241 tgtgaaagaa caaaatt
//
LOCUS       DAVBCN010000057        11977 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVBCN010000057 DAVBCN010000000
VERSION     DAVBCN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11977
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.057"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            173..2464
                     /gene="pcrA"
                     /locus_tag="VQ497_001524"
     CDS             173..2464
                     /gene="pcrA"
                     /locus_tag="VQ497_001524"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HET2517818.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTTYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            2507..3187
                     /gene="radC"
                     /locus_tag="VQ497_001525"
     CDS             2507..3187
                     /gene="radC"
                     /locus_tag="VQ497_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET2517819.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3184..3873)
                     /locus_tag="VQ497_001526"
     CDS             complement(3184..3873)
                     /locus_tag="VQ497_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET2517820.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYNLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(3891..4532)
                     /locus_tag="VQ497_001527"
     CDS             complement(3891..4532)
                     /locus_tag="VQ497_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET2517821.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(4615..4830)
                     /locus_tag="VQ497_001528"
     CDS             complement(4615..4830)
                     /locus_tag="VQ497_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET2517822.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(4838..5185)
                     /locus_tag="VQ497_001529"
     CDS             complement(4838..5185)
                     /locus_tag="VQ497_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HET2517823.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFSLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(5190..6305)
                     /locus_tag="VQ497_001530"
     CDS             complement(5190..6305)
                     /locus_tag="VQ497_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2517824.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(6315..7274)
                     /locus_tag="VQ497_001531"
     CDS             complement(6315..7274)
                     /locus_tag="VQ497_001531"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET2517825.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(7387..7956)
                     /locus_tag="VQ497_001532"
     CDS             complement(7387..7956)
                     /locus_tag="VQ497_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HET2517826.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAEDYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            8084..8755
                     /locus_tag="VQ497_001533"
     CDS             8084..8755
                     /locus_tag="VQ497_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HET2517827.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            8739..9557
                     /locus_tag="VQ497_001534"
     CDS             8739..9557
                     /locus_tag="VQ497_001534"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HET2517828.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            9554..10450
                     /locus_tag="VQ497_001535"
     CDS             9554..10450
                     /locus_tag="VQ497_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2517829.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            10494..11468
                     /gene="pta"
                     /locus_tag="VQ497_001536"
     CDS             10494..11468
                     /gene="pta"
                     /locus_tag="VQ497_001536"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HET2517830.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(11784..>11977)
                     /locus_tag="VQ497_001537"
     CDS             complement(11784..>11977)
                     /locus_tag="VQ497_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2517831.1"
                     /translation="DKEKIVNALQLPYSNAKLEATNNLIKLIKRNAFGFRNFENFKKR
                     IFTALNIKKERTKFVLSRA"
BASE COUNT         3475 a         2288 c         2584 g         3630 t
ORIGIN      
        1 aggtattgag ctagtttatg aaaaaattgg gtgaaaaagt cgagtgtttt agaaacccac
       61 agtgtagtat tctagtttca atccactata ttttgctact ccccgtaaag tttctatttt
      121 ccctgatttc tgatataata gaaatattga cttcaagagt aaggaagaga agatgaacgc
      181 attattaaat ggaatgaatg accgtcaggc tgaggcggtg caaacgacag aaggtccctt
      241 gctaatcatg gcaggagctg gttctggaaa gactcgtgtt ttaacccacc gtatcgctta
      301 tttgattgat gaaaagctgg tcaatccttg gaatatcttg gccattacct ttaccaacaa
      361 ggctgcgcgt gagatgaaag agcgtgctta tagcctcaat ccagctactc aggactgtct
      421 gattgcgacc ttccactcca tgtgtgtgcg tattttgcgt cgcgatgcgg accatattgg
      481 ctacaatcgt aattttacaa ttgtggatcc tggtgaacag cgaacgctca tgaaacgtat
      541 tctcaaacag ttgaacttgg atcctaaaaa atggaatgaa cgaactattt tggggaccat
      601 ttccaatgct aagaatgatt tgattgatga tgttgcttat gctgctcaag ctggcgatat
      661 gtatacgcaa attgtggccc agtgttatac aacctatcaa aaagaacttc gtcagtctga
      721 atccgttgac tttgatgatt tgattatgct gaccttgcgt ctctttgatc aaaatcctga
      781 tgttttgacc tactaccagc aaaaattcca atacatccac gttgatgagt accaagatac
      841 caaccacgct cagtaccaat tggtcaaact cttggcttcc cgttttaaaa atatctgtgt
      901 ggttggggat gcggaccagt ctatctacgg ttggcgtggt gctgatatgc agaatatctt
      961 ggactttgaa aaggattacc ccaaagctaa ggttgttttg ttggaggaaa attaccgctc
     1021 aaccaaaacc attctccaag cggccaacga ggttattaaa aataataaaa atcgccgtcc
     1081 taaaaatctc tggactcaaa acgctgatgg ggagcaaatc gtttactatc gtgctgatga
     1141 tgagctggat gaggctgtat ttgtagccag aaccatcgat gaacttagtc gcagtcaaaa
     1201 cttccttcat aaggattttg cagttctcta tcggactaat gcccagtccc gtacaattga
     1261 ggaagccctg ctcaagtcta acattcctta taccatggtt ggcggaacca aattctacag
     1321 ccgtaaggaa attcgcgata ttattgctta tctcaacctt attgctaatt tgagtgacaa
     1381 cattagtttt gagcgtatta tcaacgagcc taaacgtgga attggtctag gtacagttga
     1441 gaaaatccgt gattttgcaa atttgcaaaa tatgtctatg ctggatgctt ctgctaatat
     1501 tatgttgtct ggtatcaagg gtaaggcagc ccaatctatc tgggattttg ccaatatgat
     1561 gcttgatttg cgggagcagc tagaccactt aagcattaca gagttggttg agtccgtcct
     1621 agaaaaaaca ggttatgtcg atattcttaa cgcccaagcg actctagaaa gcaaggcaag
     1681 ggttgaaaat atcgaagagt ttctttctgt tacgaagaac tttgatgaca ccactgatgt
     1741 gacagaagag gaaactggtc tggacaaact gagtcgtttc ttaaatgact tggctttgat
     1801 tgccgacaca gattcaggta gtcaggagac atcagaagtg accttgatga ccctgcatgc
     1861 tgccaaaggt ctcgaatttc cagttgtctt tttgattggg atggaagaaa atgtctttcc
     1921 acttagtcgt gcgactgaag atccagatga attagaagaa gagcgccgtc tagcctatgt
     1981 aggtatcacg cgtgcagaga aaattctcta tctgaccaat gccaactcac gcttgctttt
     2041 tggtcgtacc aattataacc gtccgactcg ttttattaac gaaatcagtt cagacttgct
     2101 tgagtatcaa ggtctggctc gtcctgcaaa tacaagcttt aaggcatcat atagcagtgg
     2161 tagtatttcc tttggtcaag gtatgagttt ggctcaggct cttcaagacc gtaaacgcgg
     2221 tgctgcccca aaatcgatcc agtcaagcgg tcttccattt ggtcaattta cagctggcgc
     2281 aaaatcagca tctagcgagg caaattggtc cattggtgac attgctctcc acaagaaatg
     2341 gggagaggga actgttctgg aagtttcagg tagcggtgct aggcaggaat tgaaaatcaa
     2401 tttcccagaa gtaggtttga aaaaactttt agccagtgtg gctccaattg agaaaaaaat
     2461 ctaattttcc atccttctca cgaataataa agtgaggagg atttttatgt acagtatttc
     2521 attccaagaa gattcactat taccaagaga aaggctggcc aaggaaggag ttgaagcgct
     2581 tagtaaccaa gagttgctag ctattttact caggacagga acacgtcaag ctagcgtttt
     2641 tgaaattgcc caaaaagtct tgaacaatct ttcaagccta acggatttga aaaaaatgac
     2701 cctgcaggaa ttgcagagtt tgtctggtat tgggcgtgtt aaggccatag aattacaagc
     2761 tatgattgaa ctggggcatc gtattcacaa acacgagact cttgaaatgg aaagtattct
     2821 cagcagtcaa aagttggcca agaagatgca gcaggaatta ggggataaaa aacaagagca
     2881 cctggtggca ctctatctca atactcaaaa tcaaatcatc catcagcaga ccatttttat
     2941 cggttctgta actcgtagta tcgctgaacc gcgagagatt cttcactatg caatcaagca
     3001 tatggcgact tctcttatct tggtccacaa tcatccttca ggagcggtag cgcctagcca
     3061 aaatgatgat catgtcacta aacttgttaa agaagcctgc gaattgatgg ggattgttct
     3121 cttggaccat ttgattgtct ctcattctaa ttactttagt tatcgtgaaa agacagattt
     3181 aatctaaagt tcattaacga catagtcaaa gagtttttta tctttgggac gattttcaaa
     3241 aagaagttct ggatgccatt ggacaccgag aaaggcgaca tcatccgtac tcatgacagc
     3301 ctcaatgata ccatctttag gatcatgagc cacaactttt aaatttggtg ctaagtcctt
     3361 gatgctctgg tggtggaagg agttgatatg agagatttct ccatagattt cttggagaac
     3421 ggtatctggt tctgttacca agcgttgagt tgtgtactca acagaagaat cctgccaatg
     3481 gtcttcgata tcttggtaca aagttccacc catggcaacg ttaaagagtt gggtaccacg
     3541 gcagacagag aaaatgggct ttttctgttt aatagcttcc ttgatgaggg ccagttcgaa
     3601 gatatctctt tgaaggttat agtcatcgct atcaatggtt ttgggttcgc cataaaattt
     3661 tggatcgaca ttttgcccac ctgtcaagat gagcttgtca atcaaactga tatagtggca
     3721 ggccatttct tgatcaccaa tcggtaggat gatgggaatc cctccagcat ctttaacgcc
     3781 ttcaacaaag ccttttgctg cgtagctcat catgatgtca tcatctggat gagctttttc
     3841 gtttcctgta atcccaataa ctggtttttt cataaaatga ttttcgcttt ctaatcctct
     3901 tttcgcatga agtagaggag ggtttggagt tcacttgtca aatcgacata ctgaacgacc
     3961 acgtcttttg gtaaatgcag atggactggt gaaaaactga gaattccttt cacaccagca
     4021 tcaaccaaga gattagcaac ctcttgtgac ttgacgctgg gaacagttag gatagcagtc
     4081 ttcacatcag catccttgat tttatccttg atctgagaaa tcccgtaaat gggaatcccg
     4141 tcaggagttt gggtaccgac ttcaggatgg tcgtctaggt caaaggccat gataatcttc
     4201 atcttgttac gttcgtggaa gcggtagtgg agaagggcat ggcccatatt tccaatacca
     4261 accagcatga cattggtaat agagttgtca ttgagcaaat cggcaaaaaa tgtcattagt
     4321 tttttgacat catagccaaa accacgacga ccaagttcac caaaatagga aaaatcacga
     4381 cgtacggtcg ctgaatcaat accgatagcc tctgcaattt gcttagagtt ggcacgttca
     4441 atcttttctg catgaaatct cttaaaaatt cgatagtaga gagagagtct ttttgctgta
     4501 gcttttggaa tagcaaactg tttatctttc acaaaatcac aacctttcta ttcttctatt
     4561 ttatagaaac attgtgaaaa aatcaacaaa aataagaaaa aactaagaaa aatcttagtt
     4621 ttgatgtaaa aaatctgcat gagatagaaa acggtagagg tctccgacca gaccctgata
     4681 aactttttgg cccctaaaag tcagagaagt cacataaagt gtatctggta aggttacaca
     4741 tcctgacaaa gtcaacatga gagcctcatg atcctcatac ttgagagtac gctctacatg
     4801 atagcagtcc ttataggtca gttcaaacat tttggctcta tctttccgat tttgtaaaga
     4861 caccacgttc taccaagcta tccatgagga agtagaattt ttcctgatga atatggtggt
     4921 cttctgattt gaaaatatca actagacgaa ggctaaactt gtcagtgata ttgattttag
     4981 cccctgtaag ttccttgtta atgatgattt tgagttggaa gccttcaccg ctgtttggca
     5041 ctttttccaa aaggcgagtc agttcatagt taccaacctt agtttcaaaa aaggtgttat
     5101 ctttgagggt gaatttttta acagaagggc taagagtgta atcgtaacga caatttttta
     5161 actgaatgat tttttcaaat gccatatggc taacctccga taatttcttt taaggttttt
     5221 gcgagggttt gtaggtcttc aacggtattt tgtggcgaca aactgatgcg aagggattcc
     5281 ttcaagcgtt ctgaatttgc gccatacatg gcttcaagaa catggctgga ttggacaacg
     5341 cctgcagtac aggctgagcc agtagagatt gaaattccag ctaaatctag ccgaaggagt
     5401 aagaggtcat ttttctgacc aggaaatcca atattgagaa cataagggag atgatgtttt
     5461 cctctattca agtaatactg aatgccctcc agctctgcca gaaaggcggt ttctagattt
     5521 tgtacatgtt gaaaatgttc ttcttgtttt tctaggtctt cttttagggc tgcaaccatg
     5581 cctacgatag caggcagatt ttcagttcct gcacgttttt tctgttcctg gtctccgcca
     5641 tgtagatagg aatcaaagtc catgctagat gcgtagagaa aaccgattcc cttaggacca
     5701 tggaatttgt gggcagaagc agtgagaaaa tcaatgccca attcttctga atgaattggg
     5761 attttaccaa tagcctgaac tgcatcaaca tgataggcag cagggtgttg cttgagtatt
     5821 tggccaattt cagcgatggg cagtaggttt cctgtctcat tattggcaaa catggtagaa
     5881 accaaaatcg tatcgtcacg taaagccttt tgaatttgct gggctgtgat ttcttgattt
     5941 tctggctgga taatggttgc ttcaaaccca aagtgttgaa ccaagtaatc aattgtttca
     6001 aggacagcat ggtgctcgat ggcagttgtg atgatatgtt ttccttgttc ttggtgacga
     6061 agacagtagc caatgatggt agtattattg ccttcagtcc caccagaagt gaaaaagata
     6121 tgttgaggtt ttgtccttag taactgggct agttcctgac gggcttctcg caagagtttg
     6181 ccagcttgac gaccatgacc atgaatacta gaaggatttc cgtgggtttc ttgcataacc
     6241 ttggtcatag ctgaaatagc aactgctgac ataggagtcg ttgcagcatt gtccaaataa
     6301 atcaaagaat caccttattt ctttttattg taggcaaaga gtgggctgac tggttttctt
     6361 tcgtgaatac ggacgatagc atcaccaatt aactcactag cagtgatgta gcatacattt
     6421 ttaggagttt tttcttttgt tgctactgaa tcagtcacaa gaatttcttt aatattagta
     6481 ttgtcaagaa gctcagcagc tccctcgacg aagagaccgt ggctagaaac agcataaatt
     6541 tctgtagctc cttcacgttc aacgatttta gaagcttcag agaaggtacg tcctgtattt
     6601 aaaatatcat caatcaagat agctttctta ccttcaacat caccaataat ataaccttcg
     6661 ttacgagttg catcgtcttg agggtagtcg ataatggcga taggagcatc aagatattca
     6721 gccaggctac gcgcacgttt gacacctgaa tttttagggc taacgacaac aacatctgaa
     6781 ccaagcaatc ctttatcgca gtaatgtttt gcgaataggg gaacagtgaa aagattatcc
     6841 actggaatat caaagaaacc ttgaacctga acggcatgca aatcaagagt caggatacga
     6901 tcaactccag ccttaaccag catattggca actagttttg ctgtaagtgg ctcacgagga
     6961 caagcaatgc ggtcttgacg tgcatagcca aaatatggaa ggacaacgtt gatactgtgg
     7021 gcacttgcac gcacacaagc atcgaccatg attaacaatt ccattaggtg gttgttgaca
     7081 gggaaacttg ttgattggat gatgtaaaca tcataaccac ggacactttc ttcgatattt
     7141 acttggattt ctccgtctga aaattgacgt gatgatagtt ttccaagtgg gacaccaaca
     7201 gcttgggcaa ttttttgtgc aatctcttgg ttagagttga gtgcgaaaag tttcatgttt
     7261 tttctatctg acattataga ccgtcctctg taaactttat aaatcctagt tatatttacc
     7321 ttacatatat gaactgggat ttgtgtattt ttatcttttc tattttacca aaaaatggag
     7381 attatttcag ctatttttca tacttttgac aaatcgaacc aattttgaag gagctttttg
     7441 gtaggaaatc tgatttttct ctaaaaattg tcggaaatcc tgtttgcctt gctcatgatt
     7501 ttccacttca agctccaatt cgtaatctgt tatatcaaag tatcggctct gatccagtgc
     7561 catgagacca atagctgttt tcatttcata gcgaagcgtt gttagacaac caagaacctg
     7621 ccagttctta ctttggatac catgtttcgc caattcatcc agtactagtc cttgaggaag
     7681 ttcttcctta ctcagatagt cctcagcatc ttttagttgc aatttttggt tgtattccat
     7741 gtttccaaca ctctgcggga ctttgagtgt caactcagcc cagtcttcaa aggttcgaat
     7801 gcgcatagcg actttctttt ctcgcagttc aaaatcaggc gtgtcgatgt agtaatttgt
     7861 ttgaagaaca ggagtgacac ctgtgaactg gtcttttaga cgattgtatt catctttttt
     7921 caatagtgtt ttcaattcaa tttctaaatg tttcattttt cttacctttt tttatcgttg
     7981 aaagcggatt tatggtataa taagcattgt atttattgta tatgaatctg gagaaaaaat
     8041 caaagatatt tttgacggat aatatgagaa caagggagaa tatatgacct tagaatggga
     8101 agaatttcta gatccttaca ttcaagctgt tggtgagtta aagattaaac ttcgtggtat
     8161 tcgtaagcaa tatcgtaagc aaaataagca ttctccaatt gagtttgtga ccggtcgagt
     8221 caagccaatt gagagcatca aagaaaaaat ggctcgtcgt ggcattactt atgcgacctt
     8281 ggaacacgat ttgcaggata ttgctggctt acgtgtgatg gttcagtttg tagatgacgt
     8341 caaggaagta gtggatattt tgcacaagcg tcaggatatg cgaatcatac aggagcgaga
     8401 ttacattact catagaaaag catcaggcta tcgttcctat catgtggtag tagaatatac
     8461 ggttgatacc atcaatggag ctaagactat tttggcagaa attcaaattc gtactttggc
     8521 catgaatttc tgggcaacga tagaacattc tctcaactac aagtaccaag gggatttccc
     8581 agatgagatt aagaagcgac tggaaattac agctagaatc gcccatcagt tggatgaaga
     8641 aatgggtgaa attcgtgatg atatccaaga agcccaggca ctttttgatc ctttgagtag
     8701 aaaattaaat gacggtgtag gaaacagtga cgatacagat gaagaataca ggtaaacgaa
     8761 ttgatctgat agccaataga aaaccgcaga gtcaaagggt tttgtatgaa ttgcgagatc
     8821 gtttgaagag aaatcagttt atactcaatg ataccaatcc ggatattgtc atttccattg
     8881 gcggggatgg tatgctcttg tcggcctttc ataagtacga aaatcagctt gacaaggtcc
     8941 gctttatcgg tcttcatact ggacatttgg gcttctatac agattatcgt gattttgagt
     9001 tggacaagct agtgactaat ttgcagctag atactggggc aagggtttct taccctgttc
     9061 tgaatgtgaa ggtctttctt gaaaatggtg aagttaagat tttcagagca ctcaacgaag
     9121 ccagcatccg caggtctgat cgaaccatgg tggcagatat tgtaataaat ggtgttccct
     9181 ttgaacgttt tcgtggagac gggctaacag tttcgacacc gactggtagt actgcctata
     9241 acaagtctct tggcggtgct gttttacacc ctaccattga agctttgcaa ttaacggaaa
     9301 ttgccagcct taataatcgt gtttatcgaa cactgggctc ttccattatt gtgcctaaga
     9361 aggataagat tgaacttatt ccaacaagaa acgattatca tactatttcg gttgacaata
     9421 gcgtttattc tttccgtaat attgagcgta ttgagtatca aatcgaccat cataagattc
     9481 actttgtcgc gactcctagc cataccagtt tctggaaccg tgttaaggat gcctttatcg
     9541 gtgaggtgga tgaatgaggt ttgaatttat cgcagatgaa catgtcaagg ttaagacctt
     9601 tttaaaaaag cacgaggttt ctaagggatt gctggctaag attaagtttc gaggtggagc
     9661 tattctggtc aataatcaac cgcaaaatgc aacgtatcta ttggacgttg gagactacgt
     9721 taccattgac attcccgctg aggaaggctt tgaaaccttg gaggctattg agcatccatt
     9781 agatattctc tatgaggatg accactttct agtcttgaat aaaccctatg gagtggcttc
     9841 tattcctagt gtcaatcact ctaacaccat tgccaatttt atcaagggtt actatgtcaa
     9901 gcaaaattat gaaaatcagc aggttcacat tgttaccaga ctagataggg acacttctgg
     9961 cttgatgctc tttgccaagc acggttatgc ccatgcacga ttagacaagc agttgcagaa
    10021 gaaatctatc gagaaacgct actttgcttt ggttaaggga gatggacatt tggagccaga
    10081 aggggaaatt attgctccga ttgcgcgtga tgaagattcc attattacca gacgagtggc
    10141 taaaggcgga aagtatgccc atacttcata caagattgta gcttcttatg gaaatattca
    10201 cttggtctat attcacctgc acactggtcg aacccatcaa atccgagtcc atttttctca
    10261 tatcggtttt cctttgctgg gagatgattt gtatggtggt agtctggaag atggtattca
    10321 acgtcaggct ctgcattgcc attacctatc cttttatcat ccatttttag agcaagactt
    10381 gcagttagaa agtcccttgc cggatgattt cagtaacctt attacccagt tatcaactaa
    10441 tactctataa aaactgtctc agagtataat tattatctta aaggagaaaa ctcatggaag
    10501 tttttgaaag tctcaaagcc aaccttgttg gtaaaaatgc tcgtatcgtt ctccctgaag
    10561 gggaagagcc tcgtattctt caagcaacaa aacgcttagt aaaagaaaca gaagtgattc
    10621 ctgttttgct tggaaatcct gaaaaaatta aaatttatct tgaaattgaa ggaatcatgg
    10681 atggttatga ggtcatcgac cctcaacatt atcctcaatt tgaagaaatg gtttctgcct
    10741 tggtggagcg tcgcaagggc aaaatgactg aagaagatgt acgcaaggtt ttggttgaag
    10801 atgtcaacta ctttggtgtg atgttggttt acttgggctt ggttgatgga atggtgtcag
    10861 gagcgattca ctcaacagct tcaacagttc gtccagctct acaaatcatc aaaactcgtc
    10921 caaatgtaac tcgtacttca ggagccttcc tcatggttcg tggtacggaa cgttacctat
    10981 ttggagactg tgccattaac atcaatccag atgcagaagc cttggctgaa attgccatca
    11041 actcagcaat cacagctaag atgtttggca tcgaacctaa aattgccatg ttgagctatt
    11101 ctactaaagg ttcagggttt ggtgaaagcg ttgataaggt cgttgaagca actaaaattg
    11161 ctcacgactt gcgtcctgac cttgaaatcg atggtgagtt gcaatttgat gcagcctttg
    11221 ttcctgaaac tgcagctctg aaagctcctg gaagtacggt agctggtcaa gcaaatgtct
    11281 tcatcttccc aggtatcgag gccggaaata tcggttacaa gatggctgaa cgcctgggtg
    11341 gctttgcggc tgtaggacct gttttgcaag gtttaaacaa gccagttaat gatctttctc
    11401 gtggatgtaa tgcagatgat gtttacaagt tgaccctcat cacagcagct caagcagttc
    11461 atcaatagtg aaaactataa agcgatatac tatgctatac tgtagttatg aaactatgta
    11521 cgaaaagcac tgccattaat tcctgagaac taaattactg attggtgtca aaaaggaaaa
    11581 cttccaagcg atgatatcct gtctatacac gacctataga aatctgtaat atgtatgtcc
    11641 gtaaaacgat aatttccctt tttgatttta aatgagtatg aaaagagaat tttttcatga
    11701 gaaaacgcat aatattaagt gtcttgacac ctgctattat gcgttttctg gctctttgtc
    11761 aactgtagtg ggttgaagaa aagctaagct cgagaaagga caaattttgt cctttctttt
    11821 ttgatattca gagcggtaaa aatccgtttt ttgaagtttt caaagttccg aaaaccaaag
    11881 gcattgcgct tgataagttt gatgagatta ttggtcgctt ccaatttggc gtttgaatag
    11941 ggtagttgaa gggcgttgac gattttctct ttgtcct
//
LOCUS       DAVBCN010000058        11620 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVBCN010000058 DAVBCN010000000
VERSION     DAVBCN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11620
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.058"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            458..1168
                     /gene="deoD"
                     /locus_tag="VQ497_001538"
     CDS             458..1168
                     /gene="deoD"
                     /locus_tag="VQ497_001538"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET2517832.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(1191..1715)
                     /locus_tag="VQ497_001539"
     CDS             complement(1191..1715)
                     /locus_tag="VQ497_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HET2517833.1"
                     /translation="MKIAIIGYSGSGKSTIAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(1768..2004)
                     /locus_tag="VQ497_001540"
     CDS             complement(1768..2004)
                     /locus_tag="VQ497_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET2517834.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(2072..2992)
                     /locus_tag="VQ497_001541"
     CDS             complement(2072..2992)
                     /locus_tag="VQ497_001541"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HET2517835.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            3182..3586
                     /locus_tag="VQ497_001542"
     CDS             3182..3586
                     /locus_tag="VQ497_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517836.1"
                     /translation="MKILKRYILELCFILSFALPFIKGTNADNGRCFVETYYGFTFLM
                     EHAIVTAVFIYSFLIAFLLKKRWAKWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWT
                     SLEILTQTYQLGFYLNILLGILLIIKYFKVKQ"
     gene            3802..4383
                     /locus_tag="VQ497_001543"
     CDS             3802..4383
                     /locus_tag="VQ497_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2517837.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGQKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            4380..5657
                     /locus_tag="VQ497_001544"
     CDS             4380..5657
                     /locus_tag="VQ497_001544"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HET2517838.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIRGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVHQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            5675..6337
                     /gene="deoC"
                     /locus_tag="VQ497_001545"
     CDS             5675..6337
                     /gene="deoC"
                     /locus_tag="VQ497_001545"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET2517839.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            6324..6713
                     /locus_tag="VQ497_001546"
     CDS             6324..6713
                     /locus_tag="VQ497_001546"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET2517840.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            6803..7855
                     /locus_tag="VQ497_001547"
     CDS             6803..7855
                     /locus_tag="VQ497_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HET2517841.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALNNAVKDAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            7999..9534
                     /locus_tag="VQ497_001548"
     CDS             7999..9534
                     /locus_tag="VQ497_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2517842.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            9527..10585
                     /locus_tag="VQ497_001549"
     CDS             9527..10585
                     /locus_tag="VQ497_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2517843.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            10588..11544
                     /locus_tag="VQ497_001550"
     CDS             10588..11544
                     /locus_tag="VQ497_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2517844.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         3305 a         2074 c         2663 g         3578 t
ORIGIN      
        1 agtcacatat ataatccaag gcgacgttga cgtggtttga agagattttc gaagagtata
       61 aacagaaagg tagagcgcgt gttctaattt gaacacgagt agaaaacttt tctaaaaaca
      121 aaaacgaaag gatgggtaaa ctgtattcgc tgaactgaat acgggcgact ctcctctaaa
      181 tcaaaattaa gaaaggaatt gaccccaccc taaaagtagt gggaaaaaga tagttggtct
      241 agcgagcatc gctcactgcg cccaactcct attttccctt cgctttttga tgggtttggt
      301 atctttctca atataaaata aagaaaggta gagcgtgtgt tttgatttga acacgagcgg
      361 aaaactcgga aaatagataa tctgactgaa aaatcaggat ttctcgtcag gttcctaatt
      421 ttcagtcgtt ttcttctcgc tctttgtatc ataaattatg tctatccata ttgctgctca
      481 gcagggtgaa attgctgata aaattcttct tcctggggat cctcttcgtg ctaagtttat
      541 tgcggagaat ttccttgatg atgctgtttg ttttaacgaa gtgcgtaaca tgtttggtta
      601 cactggtact tacaagggtc actgtgtatc tgtcatggga actgggatgg gaatgccatc
      661 tatttcgatt tatgcgcgtg agttaatcgt agactacggt gtgaagaaat tgattcgtgt
      721 gggaactgca ggttctttga atgaagaggt tcatgttcgt gaattagttt tggcgcaggc
      781 ggctgcaacc aactcaaaca tcgttcgtaa tgactggcca cagtacgatt ttccacaaat
      841 tgctagcttt gatttgcttg ataaagccta ccatatcgcc aaaaaacttg gtatgactac
      901 tcacgttggg aacgttttgt catctgatgt cttttactca aattactttg aaaagaatat
      961 cgagcttggt aaatggggag tcaaggctgt ggaaatggaa gcagcagctc tttactatct
     1021 tgctgcccaa taccatgttg atgcgctagc tatcatgacc atctctgata gcttggtcaa
     1081 tccagacgaa gacacaactg cagaagaacg tcaaaatacc ttcactgata tgatgaaggt
     1141 tggtttggaa accttgattg cagaataatt atagccaaaa aggaagccct ttatgaattg
     1201 taggacttcc ttttcttatc cagaaattga tctagctctc tctgatttcg aagaatagtg
     1261 actttatgtg aatattcttg gcaaagtttt tggtaatttt ctttttgagt tttgctacgc
     1321 ccatcccaaa gaatccatct gataaactcc cactcaaagc gttcagggca atctgccgcc
     1381 atactttctc tgacttttcc acggtattta agataacgct taaaggctct aaagagacag
     1441 gtcaatggcg aaaaattgag aaagatgatt tggtcagctt cttgcattcg ttcttggtag
     1501 tagcaccaag aataattacc atcgatgacc caagctttat gcttggtgag aaagtttttt
     1561 atctcggtta acatccattc gcagtcactg tcttgccaac caggttgaaa ttggagtgtg
     1621 tccatgtgca gttttggaat ggagtagtag ttagataact tttctgctat agttgactta
     1681 ccagaaccag aatatccgat aattgcgatt ttcattttct actttttcct atttggagac
     1741 aaaaaaacag cctctatgga ctgtttctta tttagcaagt ttagctgaaa gacgagcttt
     1801 atcgcggctt gctttgtttt tgtgaatcaa acctttagtt tctgctttat cgatagctga
     1861 gctagcagca cggaaaagtt cttcagatgg gtttgcttcg aaagctttta tagcagtacg
     1921 catagctgat ttttgagctg agttcttttc gttttgttta acgttcaatt cagcgcgttt
     1981 gatagctgat ttaatgtttg ccaatgttct tacctccata tttactaact ataccattat
     2041 atatgaaaac ttacgttttg acaagggaaa attacttttt taagtaaatt tcatcgattt
     2101 catggttctt gcttttatga agaatcactt ctgcacgatt tctggttggt tcaatataat
     2161 tttgcagatt tgtgagattg atactgatcc agacctgatg ggcaaaggac tccacttccc
     2221 caatcggcat ctgagtaaaa cgataatagt agctatcagg gtcgttttgg gctaggctca
     2281 gcattttcaa gaaacggtcc agataccaac tttcaatatc atccactcca gcatctacat
     2341 agatggaaaa gtcaaagaag tcagtgatat agagacgatc gttttgtgga ttttgaaaga
     2401 cattaattcc ctcaacaatt acaaaatcag cagctttgac actttgtttc ttttcgggta
     2461 cgatgtcgta aacttcatga gaatagacag gaatatctac atcttgtcca tttttgatgc
     2521 ggtccaagaa gttgagaaga gcttccatat catagctttc aggaaatcct ttacgattta
     2581 aaatcccctg ctcaatcaag gtttgattgg gatagagaaa accatcagtt gtaaccaact
     2641 caaccgtagc atctgtaaac gtacgggaca gtaggatttg aagtaggcga ctggttgtgg
     2701 attttccaac ggcaacactc ccagaaaccc caataataaa aggttgagat ttactttcac
     2761 gttggaggaa aattcctttt gaaaaggcta aatcttcctt agttcgcttg taaatctgaa
     2821 tcagatgagc caaggggaga tagatatctg taacgtcttg gagactgatt tggtcattaa
     2881 aactcttgat agattccaat tcttcttctg tcaaaggagg tgttgtcttt cgatgtaaag
     2941 attgccaagt ctggcggctg attttttcaa aatgtaaaaa ttcgttggtc atttgttttc
     3001 ccctagatat tttgtctatc ataccataaa aagctaaaaa aataaagcgg tttcttgaat
     3061 gtggtaagtc aatggtatat agtataagtg atcaatttat tgatgaaata ggagatagat
     3121 tctactgatt tataagctta taaagggggc tttttagttc acaacttata ggaggtgtac
     3181 tatgaaaatc ttaaaacgtt acatattgga actctgtttt attttaagtt ttgctttacc
     3241 ttttataaaa ggaaccaatg cagataatgg tagatgcttt gtggaaacct attacggttt
     3301 tacttttttg atggaacatg ctattgtaac agctgtcttt atctattcgt ttttaattgc
     3361 tttcttacta aaaaaacgat gggcgaaatg gattgctgct ggtagttatt gctttttagt
     3421 cctatggatt gcgacagagg gttatttctt ccgcatgtca ctagaagatt tgatacgact
     3481 ttggacaagt ttggaaatcc tgacacaaac gtatcagttg ggcttttatc taaacatttt
     3541 gttaggaatt ttgttgataa taaagtattt taaggttaag caatagaaat aataatctgc
     3601 ctgattatag atgataaact ttttgttttt atctggactg tgatatttat agttctaaat
     3661 ggtttattat ttataagtgt tgaaagagat tgaagaaaat ggcttcgtga cttgctttct
     3721 tcggtctttt tcgttttatt atcaatttgt aaacgattta caatttgagt caaattatgg
     3781 taaaatagtg tcatgagtaa aatgtattat gcagaaaatc ctgacgctgc tcacgacatt
     3841 catgagttga gagtggactt gttgggacaa aaaatgactt ttttgacaga tgcgggtgtt
     3901 tttagcaaga aaatggttga ctttggaagt caactcttgc tcaagtgtct ggatgtcaac
     3961 caaggagaga cagtcctaga tgtaggctgt ggttatggtc cattaggttt gtccttggtc
     4021 aaggtttacg gagttcaggc gaccatggtt gatattaaca ctcgtgcctt ggatttagct
     4081 cggagaaatg ctgaaaaaaa taatgcaaaa gcgacgattt tccagtccaa catctatgaa
     4141 caagttgaag gacattttga tcatgtcatt tccaatcctc ctatccgtgc gggcaagcaa
     4201 gtcgttcatg aaatcattga gaaaagtaaa gatttcttgg aaactggtgg ggatttgaca
     4261 atcgtcatcc agaaaaaaca aggggctcca agtgctaagt ccaagatgga ggatgtgttt
     4321 ggtaattgtg aaatccttaa aaaagacaaa ggatactata tccttagaag tgtgaaagaa
     4381 tgagagcagt tgatttaata caaaaaaaac gagacggtca agaactgact tcaagtgaaa
     4441 ttgaatggct ggtagaaggc tatgtgtcag gaactgttcc tgattatcag atgtctgcct
     4501 ttgctatggc tgtttatttt aaaggaatga cgactcgtga aatttctgat ttaacgatga
     4561 atatggttaa gactgggcaa gagtttgacc tgtcagccat tgatggtgtg aaagttgaca
     4621 agcattctac tggtggtgtc ggcgataagg taaccttgat tttagctcct cttgttgcta
     4681 gcttcggtgt gcctgtagca aaaatgagtg gtcgtggtct tggccataca ggcggaacaa
     4741 ttgataaatt ggagtccatt aggggctatc aagtagaacg tagtcaagag gattttattc
     4801 gtcaggttca ggatattgga gtatctgtca ttgggcaatc agaccagctg gtcaaagcag
     4861 ataagcttct ctatgccctt cgtgatgtga ccgcaactgt cgacacgatt cctttgattg
     4921 cgagttcggt gatgagcaag aaaattgcgg caggggcgga tgctattttg ctagacgtga
     4981 ctgtcggtga gggtgccttc atgaagacgg ttgatgaggc gcgtgagttg gctcaaacta
     5041 tggtagatct tggtaaggtt gttggacgga agacagtagc agtgattacg gatatgagcc
     5101 agcctttggg acgagcgatt ggaaatcgtc tggaaatact tgaagcattg gagattttgc
     5161 aaggtcaagg acgtcaggat attacccact ttatttgtga attggctcaa attatgcttg
     5221 gtttggcaaa tgttaacaag actgttgaag aagttcacca acatcttgag aatggtcaag
     5281 cactggctaa gtttgaggaa atggtgcaag cccaaggtgg agacttggaa gacctctatc
     5341 gtcctgtaaa tgtagctcat gtggtggaaa tccctgctca ggaaacgggt gtcatttcag
     5401 ctcttccagc tatggacttt ggtctttatg ccatgaggct gggggctggt cgtgcagtta
     5461 agtctgatgc cttggactat gaaacaggaa ttgtttttga aaagaagatt ggagactccg
     5521 ttcaaaaggg tgaaattgtt gcaaaagtat acacaaatga aaaaattcca cctcaactag
     5581 ttacagattt ccaaaaatgt gttaaaataa gtgatgaagt gaaaaaaatc cgtgaaattg
     5641 tagaaatcat ctcctaaatg gagggaaaac cgaaatgaaa ttaaataaat atatagatca
     5701 tacgctttta aaacaagatg caaagaaaaa acaaattgat agtttgttgt ctgaggctag
     5761 agagtatgac tttgccagtg tttgcgttaa tccgacctgg gttgaacatg ctaaaaaagg
     5821 acttgaaggc acagatgtta aggtttgcac ggtagtaggt ttccctttgg gagcaacaac
     5881 ttcagccgtg aaagcatttg agacaaaaga agctatccaa aatggtgcag atgagattga
     5941 tatggtgatc aatgttggag ctctcaaatc aggtaattta gccttggttg agtcagatat
     6001 tcgcgcagta gtggaagcaa gtggtgataa gttagtgaaa gtcattattg aagcttgcct
     6061 tctgacagac caagaaaaaa ttgttgtttg ccaattggcc caaaaagctg gggctgactt
     6121 tgttaaaaca tctactggtt tttcaactgg tggtgctacg atagcagatg ttacattaat
     6181 gcgtgaaaca gttggatctg atatgggagt caaggctgct ggtggagctc gttcttatgc
     6241 agatgctctt gcctttgtcg aagcaggtgc gacccgtatc ggaacgtcag ctggggtagc
     6301 tattttaaaa ggagaattgg cagatggcga ctactgagtt gattgaactg gcaattgaaa
     6361 ccagcaaaca tgcctatgtc ccctattctc actttcctat cggagcagtt ttagtagcca
     6421 aagacggaag tgtttacacg ggagtgaata tcgaaaatgc tagctatcct ttgaccaatt
     6481 gcggtgagag aacagctatt tttaaggcta tatctgaagg ccaaagagaa ttttcagaat
     6541 tgattgtcta tggtcaaact gaaaaaccga tctcgccatg tggtgcttgt cgccaagtga
     6601 tggctgaatt ttttgaacaa gatctaaaag tgaccttagt cgcaaaagat aaatcgacgg
     6661 tcgagatgac ggtcggggaa ttacttccat actcttttac agacttaaac tagtctgagt
     6721 cgctctctga gtggcacggt ccttgtggcc aaccaatcca tacttgcaac atcgttgcac
     6781 atcttattta ggaggttcag taatgaacaa gaaacaatgg ctaggtcttg gcctagttgc
     6841 agtggcagca gttggacttg ctgcatgtgg taaccgctct tctcgtaacg cagcttcatc
     6901 ttctgatgtg aagacaaaag cagcaatcgt cactgatact ggtggtgttg atgacaaatc
     6961 attcaaccaa tcagcttggg aaggtttgca ggcttggggt aaagaacaca atctttcaaa
     7021 agataacggt ttcacttact tccaatcaac aagtgaagct gactacgcta acaacttgca
     7081 acaagcggct ggaagttaca acctaatctt cggtgttggt tttgccctta ataatgcagt
     7141 taaagatgca gcaaaagaac acactgactt gaactatgtc ttgattgatg atgtgattaa
     7201 agaccaaaag aatgttgcga gcgtaacttt cgctgataat gagtcaggtt accttgcagg
     7261 tgtggctgca gcaaaaacaa ctaagacaaa acaagttggt tttgtaggtg gtatcgaatc
     7321 tgaagttatc tctcgttttg aagcaggatt caaggctggt gttgcgtcag tagacccatc
     7381 tatcaaagtc caagttgact acgctggttc atttggtgat gcggctaaag gtaaaacaat
     7441 tgcagccgca caatacgcag ccggtgcaga tattgtttac caagtagctg gtggtacagg
     7501 tgcaggtgtc tttgcagagg caaaatctct caacgaaagc cgtcctgaaa atgaaaaagt
     7561 ttgggttatc ggtgttgatc gtgaccaaga agcagaaggt aaatacactt ctaaagatgg
     7621 caaagaatca aactttgttc ttgtatctac tttgaaacaa gttggtacaa ctgtaaaaga
     7681 tatttctaac aaggcagaaa aaggagaatt ccctggcggt caagtgatcg tttactcatt
     7741 gaaggataaa ggggttgact tggcagtaac aaacctttca gaagaaggta aaaaagctgt
     7801 cgaagatgca aaagctaaaa tccttgatgg aagcgtaaaa gttcctgaaa aataatggat
     7861 ggaagtcatt tcttaggaag cggccaaggg ccgctttttt aagagttgag acaaagtcat
     7921 tttgtctcag taaagcttgc tactagacaa actagcaagt tttattgaaa taaaataaga
     7981 ctctgaaagg aagagcacat ggcacacgaa aatgtcattg agatgcgtga tattaccaag
     8041 gtgtttggtg gatttgttgc caacgacaaa atcaacttgc acctacgaaa aggtgaaatt
     8101 catgcacttt taggagaaaa tggggctggt aagtccacgc taatgaacat gttagcaggc
     8161 cttcttgaac caactagtgg tgaaatcgcg gtcaacggtc aagttgtcaa tctcgactcc
     8221 ccatctaaag cagctagctt gggaatcggg atggttcacc agcactttat gttggttgaa
     8281 gccttcacag tggctgaaaa catcatttta ggtagtgaat tgactaaaaa tggtgtgcta
     8341 gatatcgctg gagctagcaa agaaatcaag gctctttctg aacgttatgg cttagctgtt
     8401 gacccttctg ccaaggtagc agatatctca gttggagccc aacaacgtgt agaaatttta
     8461 aaaacacttt atcggggggc tgatatcctt atctttgacg aaccaacggc tgttttgact
     8521 ccatcagaaa ttgatgagtt gatggctatt atgaaaaatc ttgtcaaaga aggaaaatca
     8581 attatcttga ttacccacaa attggatgaa attcgcgcag tttctgaccg tgttacagtt
     8641 atccgtcgtg ggaaatcaat tgaaaccgtt gaaattgcag gggctaccaa tgctgatttg
     8701 gcggaaatga tggtaggacg ttctgtttcc tttaaaacag agaagcaagc ctctaaacca
     8761 aaagaagtgg ttttgtctat caaagatttg gtggtcaatg aaaaccgtgg tgttccagct
     8821 gttaaaaatc tgtccttgga tgttcgtgct ggagagattg ttggtattgc ggggattgat
     8881 ggaaatggtc agtctgaact gattcaagcc attacaggtc ttcgtaaggt tgaatctggt
     8941 agcattgagc taaaaggaga ttcaattgta ggcttgcacc cacgtcagat tacagaacta
     9001 agtgttgggc acgttccaga agaccgtcac cgtgatggct tgattttgga aatgatgata
     9061 tctgaaaata ttgcccttca aacctactat aaagaaccac atagtaaaaa tggaattttg
     9121 aattattcaa atattacttc ttatgctaaa aagctgatgg aagagtttga tgttcgcgct
     9181 gccagtgaat tagttcctgc agctgcactc tcaggaggaa atcaacaaaa agcaattatt
     9241 gctcgtgaaa ttgatcgaga tcctgatctc cttatcgtta gccagccaac tcgtgggttg
     9301 gatgtcggtg ccattgagta tatccacaaa cgcttgattg aagagcgtga taatggcaag
     9361 gctgtccttg ttgtcagctt tgaattggat gagattttaa acgtctcaga ccgtattgcc
     9421 gttatccacg atggtaagat tcaaggtatt gtatcaccag aaacaaccaa taaacaagaa
     9481 cttggtgtct tgatggctgg tggaaacttg ggaaaggaga agagtgatgt ctaaaaaatt
     9541 acaacaaatt tcggttccct tgatttctgt atttctagga attttactcg gagccattgt
     9601 catgtggatc ttcggttatg atgctatttg gggctacgaa gaattgttct atacagcctt
     9661 tggcagtctg cgtgggattg gagaaatctt ccgtgctatg ggtcctctgg tcttgattgg
     9721 tcttggtttt gcggttgcca gtcgagctgg tttctttaac gtcggacttc ctggtcaggc
     9781 tttggcaggt tggattctca gtggttggtt tgcgctgtcg catccagata tgccccgtcc
     9841 cttgatgatt ctagcaacca tcgtgattgc cttgattgct ggtgggattg tcggagcgat
     9901 tccaggtatt cttagggcct atctagggac gtcagaggtt attgtaacca tcatgatgaa
     9961 ctacattgtc ttgtatgtag ggaatgcctt tatccatgct ttccctaaag acttcatgca
    10021 aagtacagat tcgaccattc gtgttggggc taatgcaacc tatcagacac cttggttggc
    10081 tgagttgact ggtaactcac ggatgaatat tggtattttc tttgccatca ttgccgttgc
    10141 agttatttgg ttcatgctca agaaaacaac tcttggtttt gaaatccgtg cagttggtct
    10201 taatccacat gcttcagaat atgctggtat ttctgccaag cggactatta tcctatctat
    10261 gattatttca ggtgccttgg caggtcttgg tggagctgtt gaaggtttgg gaaccttcca
    10321 gaacgtctat gttcaaggtt cgtcattagc tatcggattt aacggaatgg cggttagttt
    10381 gcttgcggcc aactcaccaa ttggtatact ctttgcagcc ttcctatttg gcgttctcca
    10441 agttggggct cctggtatga atgcggcgca ggtaccatct gagcttgtca gcattgtaac
    10501 agcgtctatt atcttctttg tcagtgttca ttaccttatc gaacgctttg tcaaaccgaa
    10561 aaaacaagtt aaaggaggta agtaaagatg tctattataa ccttgctccc cctcttggtg
    10621 tcttctatgc tgatttactc agcacctctc atctttacaa gtatcggtgg tgttttctct
    10681 gaacgtggtg gtgtggtaaa cgttggcctt gaaggaatta tggttatggg tgccttttct
    10741 ggagttgtct ttaaccttga atttgcagaa caatttggag cagcaactcc atggctatcc
    10801 ttgcttgtag caggattggt tggtagtgtt ttctctatca tccacgcagc agcgacggtt
    10861 catttccgtg cagaccatgt tgtcagcggt acggtattga acttgatggc gcctgccttg
    10921 gctgttttct tagttaaagt tctttataac aaaggacaaa ccgacaacct aagtcaaact
    10981 tttggacgct ttgatttccc agtcttggca aatatcccag tgattggtga tatcttcttc
    11041 aagtcaacta gtctacttgg ttatctggcg attgccttct cattccttgc ttggtttatt
    11101 ctctttaaga ctcaatttgg tcttcgtctc cgctctgtcg gtgaacatcc tcaagcagcg
    11161 gataccttgg gaatcaacgt ctacaagatg agatatttag ggattattat ttcaggtttt
    11221 ctaggtggaa ttggcggagc gatttatgcg caatcaatct cagttaactt ctcagtgaca
    11281 actattgttg gacctggatt tatcgccctt gctgcgatga tctttgggaa atggaatcca
    11341 atcggtgcta tgctttctag tctcttcttt ggactttcac aaagtttggc tgttatcggt
    11401 tctcaattgc cgttcctaca aggagtgcca gcggtttatc ttcaaattgc accttatgtt
    11461 ttgacaattc ttgtcttagc agccttcttt ggaaaagcag tcgcaccaaa agcagatggt
    11521 atcaactaca tcaaatcaaa ataagcatac aaaaaaacgt cagtccaatg actgaactgc
    11581 cccccaaaag ttagacagaa aaaatctaac ttttggggtg
//
LOCUS       DAVBCN010000059        11527 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVBCN010000059 DAVBCN010000000
VERSION     DAVBCN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11527
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.059"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            183..437
                     /gene="blpM"
                     /locus_tag="VQ497_001551"
     CDS             183..437
                     /gene="blpM"
                     /locus_tag="VQ497_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HET2517845.1"
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            453..656
                     /gene="blpN"
                     /locus_tag="VQ497_001552"
     CDS             453..656
                     /gene="blpN"
                     /locus_tag="VQ497_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HET2517846.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            900..1049
                     /gene="blpO"
                     /locus_tag="VQ497_001553"
     CDS             900..1049
                     /gene="blpO"
                     /locus_tag="VQ497_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HET2517847.1"
                     /translation="MDTKMMSQFAVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            1153..1272
                     /locus_tag="VQ497_001554"
     CDS             1153..1272
                     /locus_tag="VQ497_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HET2517848.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            2058..2456
                     /locus_tag="VQ497_001555"
     CDS             2058..2456
                     /locus_tag="VQ497_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HET2517849.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEVGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKKQTVFLILQQ"
     gene            2494..3183
                     /locus_tag="VQ497_001556"
     CDS             2494..3183
                     /locus_tag="VQ497_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2517850.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNFV
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            3225..3473
                     /locus_tag="VQ497_001557"
     CDS             3225..3473
                     /locus_tag="VQ497_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517851.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLKWFVLERLELKDDA"
     gene            3503..4114
                     /locus_tag="VQ497_001558"
     CDS             3503..4114
                     /locus_tag="VQ497_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2517852.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            4275..5069
                     /locus_tag="VQ497_001559"
     CDS             4275..5069
                     /locus_tag="VQ497_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET2517853.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            5066..5701
                     /gene="trmB"
                     /locus_tag="VQ497_001560"
     CDS             5066..5701
                     /gene="trmB"
                     /locus_tag="VQ497_001560"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HET2517854.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            5827..6306
                     /gene="rimP"
                     /locus_tag="VQ497_001561"
     CDS             5827..6306
                     /gene="rimP"
                     /locus_tag="VQ497_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET2517855.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            6350..7486
                     /gene="nusA"
                     /locus_tag="VQ497_001562"
     CDS             6350..7486
                     /gene="nusA"
                     /locus_tag="VQ497_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HET2517856.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            7508..7801
                     /locus_tag="VQ497_001563"
     CDS             7508..7801
                     /locus_tag="VQ497_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HET2517857.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            7794..8093
                     /locus_tag="VQ497_001564"
     CDS             7794..8093
                     /locus_tag="VQ497_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HET2517858.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            8110..10902
                     /gene="infB"
                     /locus_tag="VQ497_001565"
     CDS             8110..10902
                     /gene="infB"
                     /locus_tag="VQ497_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET2517859.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            11153..11503
                     /gene="rbfA"
                     /locus_tag="VQ497_001566"
     CDS             11153..11503
                     /gene="rbfA"
                     /locus_tag="VQ497_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET2517860.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         3522 a         2017 c         2622 g         3366 t
ORIGIN      
        1 gctggtatta tcaatcatga actaggattc tagttttgca ggaagtctaa tgtaattttt
       61 gcaattcaag acgtttcgat gactattcaa gatctggatg aaaaatttta aaaaacgatg
      121 atatactaaa cttgtcaaag ttacaacaag acaaaaatta aaaataaaaa agagtatttg
      181 tcatggatac aaaaattatg gaacaatttc atgaaatgga tataactatg ttatctagta
      241 ttgaaggagg caagaataat tggcaaacta atgtcttgga aggtggtggt gctgcttttg
      301 gtggctgggg gctagggaca gctatttgtg ctgcgagtgg tgttggagca ccatttatgg
      361 gagcatgtgg atacatagga gctaaatttg gtgtggatct ttgggcagga gtaacagggg
      421 caacgggtgg attttaataa aaggagactt gtatgaatac ttattgtaat ataaatgaaa
      481 caatgctgag tgaggtttat ggaggtaatt ccggaggagc agctgtagtt gctgctttag
      541 gttgtgcagc gggtggagtg aaatatggga gacttctagg accatggggc gctgcaatag
      601 gaggaattgg aggagcagtg gtttgtggat atttagccta taccgctaca tcataaaaat
      661 gaaagctaaa ttttatgaag gctatattct ttattattct cttcgctttt caaacctatc
      721 ttatttatct atccataagt atctccgata aaaaacaaaa gactattgaa ttaacatcat
      781 tgaattgttt tgtcatatta tttctaatct atgataaatt aatttttctc tttattgcct
      841 atgttttttt gataattttt atattaaact tgttccgtaa ctgaaaagtg atatactata
      901 tggatacaaa aatgatgtca caatttgcag ttatggataa tgaaatgctt gcttgcgttg
      961 aaggtggaga tattgattgg ggaagaaaaa ttagttgtgc agcaggggtt gcatatggcg
     1021 caattgatgg gtgtgcaaca acggtttgac atttctattg ggaccatttg ctataggaat
     1081 aggtgtaact ggtgctgcag gtggagctat acttggaggt gtagcctatg cagcaatatg
     1141 ttggtggtaa ttatggattt taaaagtttt attattggtt tagtagttgg tatatttggt
     1201 ccttatatgg atgacttaat tagaaaaata ttttcaaaat cttccaaaaa ggatacggat
     1261 agtactctct aaaaaataaa tttctcccct gtatttgcta gcttaattta caggggagtt
     1321 tctttaatat tgtttcgcct gtcaaaatga tatgtgatag caggatgaaa agtctacaaa
     1381 caagttggta tcaatacaat ataaagagga aagtatgttt ttaatgctta ctttttaaat
     1441 tttttaacaa ttcaagatgt ttcgatgaca attcaagatt tggatgaaaa attttaaaaa
     1501 acaatgatat actaaacttg tcaaagttgc aacgggtgct gctacattat gttaaaaatc
     1561 gctcttgatt gataagctgg atttgttagg aaaggagtaa ataattatgg ataagaaaaa
     1621 gatagtttcg actataatat gtatagtatt tcttgtagtg tcagtagata atttttttag
     1681 agacttaacc ccgttactgt tcattttaaa tattattggc ctgtcttgct tttcagtgct
     1741 aacatacatt aacataaaag agatactctt aaatataagt aaatgatgat ttggttagtc
     1801 ctagaaccaa tcaacttatg tttatcggct tggcagattt tatgtttgtc atttgtttgt
     1861 atagaggtat ttcagaaaca gaattctatc aacaactaat cgcttatata ggtgtattct
     1921 ctgcttgttt atcacgcttc tgctcatgtg gggcttgaag tattataaaa agtagctttc
     1981 tatccttttc tgacaggata tttgagtaga aggctctatt aaattgttag tgaaataaat
     2041 gaatggaggt atttaatatg aaatacagat tattttttgt tattttcttg agtagtgtgt
     2101 tggatattct tttagggaca tttttacaaa tctctatcgt atctattggg tggcttgttc
     2161 tttacagtgg tttgtttgaa gtaggagttt tccttcttgc taataaaggg gtggcggtaa
     2221 aaatcaagga agtagatatt cgaaatcgct ttaaatttat ttttggaaaa accttatggt
     2281 ttcaaattct tttgctcatc tttttgataa tcaaacttta tcttggtttg gatgcgaggt
     2341 tgattttatt ctatggacat attttcattg tctttaatgc cttaatgtat ctattatcta
     2401 gtagtcaggt tagccttaaa aaaaaacaaa ctgtcttctt aatcttacag cagtagagtc
     2461 tgacaaggtg aggtcatagt agggaggatt tctatgaaaa agtatcaact tctattcaaa
     2521 ataagtgcag tcttctctta cttatttttc gtatttggtc tttctcagct gacgcttatc
     2581 gtccaaaatt attggcaatt ttcttcccag attggtaatt tcgtctggat tcaaaatatc
     2641 ttgagtttac tttttattgg agtcatgatt gtggttcttg ttaagacagg ccatggttat
     2701 ctcttccgca ttccaagaaa aaaatggctt tggtattcga ttttgacagt attagtggta
     2761 gtgttccaga tctcttttaa cgttcagaca gctaaacatg ttcagtcaac tgcggaaggt
     2821 tgggctgtat tgattggtta tagtgggact aactttgcag agctaggtat ttatatagcc
     2881 ctgttctttc tggttccact gatggaagaa ttgatttata gaggattact gcaacatgct
     2941 ttctttaagc attcgcgatt tggtcttgat ttgcttcttc cttctatttt atttgctctc
     3001 cctcattttt caagcctgcc tagtctgtta gatatcttcg tctttgcaac agttggaatc
     3061 atctttgctg gtttgacccg ctataccaag agcatttatc catcctatgc ggtgcatgtg
     3121 atcaataata ttgtagcgac cttcccgttt ttgctcactt ttctacatag ggtcttgggg
     3181 taaaaaaaca aaaggcttgc ttttcagcca tagaggaggt catcatgtat aaacacttat
     3241 ttttcctaga ttccaaaact ttagatcggt tgacacccta tattctagtc ttggcttctg
     3301 acaccattgc ttttaatgtt tttgtgctaa cctttgtatc tgcggtggtt tttaatttcc
     3361 taaattccat gctagcttta atggctatat tcataggggc tggctatgtg gtcggatttt
     3421 ggttgctaaa atggtttgtt ttggaaaggt tagagctaaa ggatgacgcg taggaagtct
     3481 gtttggtgag gaggatagtt ttatggagtt ttttgataaa tttcatgcct tgtgttttgg
     3541 atttttagta ctaataattg tcattacagt tccttatacg attaaccatg ggggtttttt
     3601 tcaaaatgaa tctgcattga ttcttgtaag tcttcttgta acctcgctga gtgttgctta
     3661 tgctagaaag tttgaaatga tttcttttgg gatgttaagc aagaaacaac ttttgctttt
     3721 cattgcaatc tttcttctaa gtgtacttga gacgctggtt tatattcatt tcttcgctgt
     3781 ttcttctggc tcaggggtcc aacacttggc ggaagtcagc agaggaattt ccctgtcttt
     3841 gattttgact acctcagttt ttggccccat ccaggaggaa ctcattttca gaggacttct
     3901 tcaaggtgcg gtttttgaca attcttggtt agggcttgtg ctaacttcct ctctcttttc
     3961 tttcatgcat ggaccttcta atgtcccttc gtttattttt tatctacttg ggggtttgtt
     4021 gctgggcttt gcttataaaa agagtcaaaa cctatgggtt tctactctag tacacatgtt
     4081 ttacaacagt tggccactcc tatattattt ataaaaatta tgaaaaggta agcagaagac
     4141 ggtgcttacc tttttcaagc tataatgata cccaagccaa tccagaggct tttctttgag
     4201 aatcgggtta gagtggttga cgaataggcc aaaaactagt agaatagtaa ggaaacttta
     4261 tacggaggaa agaaatggat ttgggtgata atgagctaac actgactccc atacctggga
     4321 aaagtggcaa ggcttatatg ggtagctatc ctgacgggaa gcgcatcttt gtaaaaatga
     4381 acacctctcc aatcctacct ggtctagcta gagaacaaat tgctccacaa ttattatgga
     4441 gtcgtcgttt ggcagatggg cgtgatatgt gtgctcaaga atggttgaca ggcaagatat
     4501 tgacccccta tgatatgaat cgtaagcaaa tcgtcaatat tttaacccgt cttcatcgct
     4561 cacgtccgtt gatgacacaa ttgagtcgtt tgggctatgc catggaaaca cctgtagatt
     4621 tactacagtc ttggcaggaa acggctccag atgctttgcg taaaaatcat tttatcagtg
     4681 aagtgatggc tgatttacgt cagactattc caggatttag agaggaccat gcgaccattg
     4741 tccatggaga tgtacgacat agtaattgga ttgagacaga tagtggcttg atttatttag
     4801 tagattggga ttcggttcgc ttgaccgatc gcatgtttga tgtggcccat atgctctgcc
     4861 attatatttc agaacatcag tggaaggaat ggttgaccta ctacggttac aagtacaatc
     4921 aaacggtatt aagtaaattg tattggtatg gtcaattgtc ttatttgagt cagatttcca
     4981 agtattatat gaaccaagat ttagaaaatg tcaatcggga gattcatggt ttgcgtcatt
     5041 tccgagacaa gtatggaaag agaagatgag agttagaaat cgtaaagggg caacagaatt
     5101 actagaggca aatccccagt atgtggtcct caatcccttg gaagccaagg caaaatggcg
     5161 ggacttgttt ggcaatgata atcccattca tgtggaagtt ggaagtggaa agggtgcctt
     5221 tgtttcaggt atggccaagc aaaaccctga catcaactat atcgggattg atattcaaaa
     5281 gtctgttttg agctacgctt tggacaaggt gcttgaagtt ggagtgccta acatcaagct
     5341 cttgtgggta gatggttctg acttaactga ctattttgaa gacggtgaga ttgatcgctt
     5401 gtatctgaac ttttcagatc catggccgaa aaaacgccat gaaaagcgtc gtttgaccta
     5461 caagaccttc ttggatacct tcaaacgtat cttgcctgaa aatggagaaa ttcatttcaa
     5521 gacggataac cgtggcttgt ttgagtacag tttagtgagc ttttctcaat atggcatgaa
     5581 actcaatggt gtctggttag atttgcatgc cagtgatttt gaaggcaatg tcatgacaga
     5641 atacgagcaa aaattctcaa acaaggggca agttatctac cgagttgagg cagaatttta
     5701 agagatagcc taaaattagg ctgtacaagt gcttttgctt tacataagtt ggcaaacgtg
     5761 ctatactgat agtaagaata tgaaaagtga ggcggggaaa tatcttcgcc tcttgcttat
     5821 gaggaggtgg acgcaatcgc aacaatcgta gaattagtca gagaagttgt agaacctgtc
     5881 atagaagctc cttttgaact cgtggatatc gagtatggaa agattggcag tgacatgatt
     5941 ctcagtattt ttgtagataa acccgaagga attaccttga acgacacggc agacttgaca
     6001 gaaattatca gtcctgtcct agacaccatc aagccagatc ccttcccaga acaatatttc
     6061 ctagaaatta ccagtccagg tttggaacgc cctttgaaaa ccaaggatgc cgtcgctgga
     6121 gcggttggaa aatacatcca tgtcgggctc taccaagcca tcgataagca aaaggtcttt
     6181 gaaggaacct tgttggcctt cgaagaggac gagttgacta tggaatatat ggacaagacg
     6241 cgtaagaaaa ccgtccaaat tccatacagt ttagtatcaa aagcacgttt agcagttaaa
     6301 ttatagaaaa agaaaggata gcttttgagg attcaaaagt gaagaaaaca tgagtaaaga
     6361 aatgctagag gccttccgca ttttggaaga agacaaggga atcaaaaaag aagatatcat
     6421 cgacgcagta gtagagtcgc ttcgttccgc ttatcgcaga cgctatggtc agtcagacag
     6481 cgtagctatt gacttcaacg aaaaaacagg tgactttaca gtttatactg tccgtgaagt
     6541 tgttgatgaa gtatttgata gccgtttgga aatcagcttg aaagatgctc ttgccattaa
     6601 ttcagcttat gaacttggag acaaaatcaa gtttgaagaa gcaccagctg agtttggtcg
     6661 tgtagcagcc cagtctgcca aacaaaccat catggaaaaa atgcgcaagc aaacacgtgc
     6721 catcacttac aatacttaca aagaacatga gcaagaaatc atgtctggta cagtagaacg
     6781 ctttgacaac cgctttatct atgtcaacct tggtagcatc gaagcccaat tgtcaaaaca
     6841 agaccaaatt cctggagaag tttttgcttc tcatgatcgt atcgaagttt atgtttacaa
     6901 ggttgaagac aaccctcgtg gtgtgaacgt ctttgttagc cgtagtcatc cagaaatgat
     6961 caaacgttta atggagcaag aaattccaga agtttatgat ggaactgttg aaatcatgag
     7021 cgtggctcgt gaagcaggtg accgtacgaa ggttgctgtt cgtagccaca atccaaacgt
     7081 ggatgctatc ggtacaatcg ttggacgtgg tggtgctaat atcaagaaga ttactagcaa
     7141 attccaccca gctcgttacg atgctaaaaa tgaccgcatg gtaccaatcg aagaaaatat
     7201 cgatgttatc gagtgggtag cagatccagc tgaatttatc tacaatgcca tcgctcctgc
     7261 tgaggttgac caagttatct ttgatgaaaa cgacagcaaa cgtgccttgg tggttgttcc
     7321 agataacaag ctttctcttg ccattggtcg tcgtggacaa aacgtgcgct tggcggctca
     7381 cttgactggt taccgtatcg atatcaagtc tgctagcgaa tttgaagcca tggaagacgc
     7441 tgcttcagta gagttggaag tagaaaacga tactgtagaa gaataaaagc tgctagagga
     7501 gggaaagatg aaaacaagaa aaatcccttt gcgcaagtct gttgtgtcta acgaagtcat
     7561 tgataagcgt gatttgctcc gcattgtcaa gaacaaggaa ggacaagtct ttattgatcc
     7621 tacgggcaag gccaatggcc gcggcgctta tatcaaacta gacaatgcag aagccctaga
     7681 ggcgaaaaag aagaaggtct ttaaccgcag ctttagcatg gaagtggaag aaagctttta
     7741 tgacgagttg atcgcttatg tggatcacaa agtgaaaaga agagagttgg gacttgaata
     7801 agcaaaagat aagtaatctc ttggggcttg ctcagcgagc agggcgcatc atatcgggtg
     7861 aagaattggt ggtcaaggcc attcaagacg gcaaggccaa gttggtcttt ctagctcatg
     7921 atgctggacc caatctgacc aagaagattc aagataaaag tcattattat caagtagaaa
     7981 ttgtaaccgt gttttcaaca ctggaattaa gcatagcagt cgggaaatcg agaaaggttt
     8041 tggctgtaac agatgctgga tttacaaaga aaatgaggtc tcttatggaa tagaagagga
     8101 ggacatgatt tgtctaagaa aagattgtac gaaatcgcaa aagaacttgg aaaagaaagt
     8161 aaagaagttg tagcgcgtgc aaaagagttg ggcttggatg tgaaaagcca ctcatcaagt
     8221 gtggaagaag ctgtcgctgc aaaaattgct gccagcttta agcctgcagc tgctccgaaa
     8281 gtagaagcaa aacctgcagc accaaaagta agtgcagaaa agaaagccga aaaatctgag
     8341 ccagctaaac cagctgtagc taaggaagag gcaaaaccgg ctgagccagt tgctccgaaa
     8401 acagaaaaag tagcagtgaa accgcaaagt cgtaatttca aggctgagcg tgaagcccgt
     8461 gctaaagagc aggcagaacg acgcaagcaa aataagggca ataaccgtga ccaacaacaa
     8521 aacggaaacc gtcagaaaaa cgacggccgt aatggtggaa aacaaggtca aagcaaccgc
     8581 gacaatcgtc gctttaatga ccaagctaag aagcagcaag gtcagcaaaa acgtagaaat
     8641 gagcgccgtc agcaagagga taaacgttca aatcaagcgg ctccacgtat tgactttaaa
     8701 gcccgtgcag cagccctaaa agcagagcaa aatgcagagt acgctcgttc aagtgaggaa
     8761 cgcttcaagc agtatcaggc tgctaaagaa gccttggctc aagctaacaa acgcaaggaa
     8821 ccagaggaaa tctttgaaga agcggctaag ttagctgaac aagcacagca agttcaagca
     8881 gtggttgaag tcgtccctga gaaaaaagaa cctgcagtgg atacacgtcg taaaaaacaa
     8941 gctcgaccag acaaaaatcg tgacgattat gatcatgaag aagatggtcc tagaaaacaa
     9001 caaaagaatc gaagtagtca aaatcaagtg agaaatcaaa agaatagtaa ctggaataac
     9061 aacaaaaaga acaaaaaagg caataacaag aacaaccgta atcagactcc aaaacctgtt
     9121 acggagcgta aattccatga attgccaaca gaatttgaat atacagatgg tatgaccgtt
     9181 gcggaaatcg caaaacgtat caaacgtgaa ccagctgaaa tcgttaagaa acttttcatg
     9241 atgggtgtca tggccacaca aaaccaatcc ttggatgggg aaacaattga actcctcatg
     9301 gtggattacg gtatcgaagc caaacaaaag gttgaagtgg ataatgctga catcgaacgt
     9361 ttctttgtcg aagatggtta tctcaatgaa gatgaattgg ttgagcgtcc accagttgtt
     9421 actatcatgg gacacgttga ccacggtaaa acaacccttt tggatactct tcgtaactca
     9481 cgtgttgcga caggtgaagc aggtggtatt actcagcata tcggtgccta ccaaatcgtg
     9541 gaaaatggta agaagattac cttccttgat acaccaggac acgcggcctt tacatcaatg
     9601 cgtgcgcgtg gtgcttctgt taccgatatt acgatcttgg tcgtagcggc agatgacggg
     9661 gttatgcctc agactattga agccatcaac cactcaaaag cagctaacgt tccaatcatc
     9721 gtagccatta acaagattga taaaccaggt gctaacccag aacgcgttat cggtgaattg
     9781 gcagagcatg gtgtgatgtc aaccgcttgg ggtggagatt ctgaatttgt tgaaatctca
     9841 gctaaattca accaaaatat cgaagaattg ttggaaacag tccttcttgt agctgaaatc
     9901 caagaactca aggcagaccc aacagtgcgt gcaatcggta cggttatcga agcgcgcttg
     9961 gataaaggaa aaggtgcggt cgcaaccctt cttgtacaac aaggtacctt gaatgttcaa
    10021 gacccaatcg ttgtcggaaa taccttcggt cgtgtccgtg ctatgaccaa cgaccttggt
    10081 cgtcgtgtta aagttgctgg accatcaaca ccagtctcta tcacaggttt gaacgaagca
    10141 ccgatggcgg gtgaccactt tgccgtttac gaggatgaaa aatctgcgcg tgcagcaggt
    10201 gaagagcgtg ccaaacgtgc cctcatgaaa caacgtcaag ctacccaacg tgttagcctt
    10261 gaaaacctct ttgataccct taaagctggg gaactcaaat ctgttaatgt tatcatcaag
    10321 gctgatgtac aaggttctgt tgaagccctt tctgcctcac ttcaaaagat tgacgtggaa
    10381 ggtgtcaaag tgactatcgt ccactcagcg gtcggtgcta tcaacgaatc agacgtgacc
    10441 cttgccgaag cttcaaatgc ctttatcgtt ggtttcaacg tacgccctac accacaagct
    10501 cgtcaacaag cagaagctga cgatgtggaa atccgtcttc acagcattat ctacaaggtt
    10561 atcgaagaga tggaagaagc tatgaaaggg atgcttgatc cagaatttga agaaaaagtt
    10621 attggtgaag cggttatccg tgaaaccttc aaggtgtcta aagtgggaac tatcggtgga
    10681 tttatggtta tcaacggtaa ggttgcccgt gactctaaag tccgtgttat ccgtgatggt
    10741 gtcgttatct atgatggtga actcgcaagc ttgaaacact ataaagacga cgttaaggaa
    10801 gttacaaacg gccgtgaagg tggattgatg atcgacggct acaatgatat taagatggat
    10861 gatgtgattg aggcgtatgt catggaagaa atcaagagat aagatttttt gctcctttct
    10921 taggtggtga gggacgcaag caaaccgatg gtttcattgc ttatttttga gcctagggtc
    10981 tcaaaaatcc cctgtgatgg gactgataaa tcagttccat cactttcacc acggcgaaag
    11041 aagcagatga cttcaaattg aacttcgttt caatttaaac tgaaaatcaa gaagtttaaa
    11101 atagctaggt ctgctggcct agcttttggt tcaaagtaga gaaaggaata tcatggcaaa
    11161 tcatttccgt acagatcgtg tgggcatgga aatcaagcgt gaagtcaatg agattttgca
    11221 aaagaaagtc cgtgatccac gtgtccaagg tgtgaccatc acagatgttc agatgctggg
    11281 tgacttgtct gttgccaagg tttattacac cattttgagt aaccttgctt cggataacca
    11341 aaaagcccaa atcgggcttg aaaaagcaac tggtaccatc aaacgtgaac ttggtcgcaa
    11401 tttgaaattg tacaaaatcc cagatttgac cttcgtcaaa gacgagtcca tcgagtatgg
    11461 aaacaagatt gacgagatgc tacgcaatct ggataagaac taaagaagag gggttgcccc
    11521 tcttttt
//
LOCUS       DAVBCN010000060        11392 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVBCN010000060 DAVBCN010000000
VERSION     DAVBCN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11392
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.060"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(39..2036)
                     /gene="metG"
                     /locus_tag="VQ497_001567"
     CDS             complement(39..2036)
                     /gene="metG"
                     /locus_tag="VQ497_001567"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET2517861.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEYDGKLTLLTVDPAVPNGSVIG"
     gene            complement(2160..3419)
                     /locus_tag="VQ497_001568"
     CDS             complement(2160..3419)
                     /locus_tag="VQ497_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2517862.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKKSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(3421..4122)
                     /locus_tag="VQ497_001569"
     CDS             complement(3421..4122)
                     /locus_tag="VQ497_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2517863.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(4106..5305)
                     /locus_tag="VQ497_001570"
     CDS             complement(4106..5305)
                     /locus_tag="VQ497_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET2517864.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPFQTAVKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAQAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILIPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            5591..6937
                     /gene="gor"
                     /locus_tag="VQ497_001571"
     CDS             5591..6937
                     /gene="gor"
                     /locus_tag="VQ497_001571"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HET2517865.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            7134..7670
                     /locus_tag="VQ497_001572"
     CDS             7134..7670
                     /locus_tag="VQ497_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HET2517866.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALAVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            complement(7876..8091)
                     /locus_tag="VQ497_001573"
     CDS             complement(7876..8091)
                     /locus_tag="VQ497_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HET2517867.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            8189..8509
                     /locus_tag="VQ497_001574"
     CDS             8189..8509
                     /locus_tag="VQ497_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517868.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(8540..8878)
                     /locus_tag="VQ497_001575"
     CDS             complement(8540..8878)
                     /locus_tag="VQ497_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517869.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(8890..9609)
                     /gene="trmD"
                     /locus_tag="VQ497_001576"
     CDS             complement(8890..9609)
                     /gene="trmD"
                     /locus_tag="VQ497_001576"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET2517870.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(9599..10117)
                     /gene="rimM"
                     /locus_tag="VQ497_001577"
     CDS             complement(9599..10117)
                     /gene="rimM"
                     /locus_tag="VQ497_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET2517871.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKRYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(10148..10936)
                     /locus_tag="VQ497_001578"
     CDS             complement(10148..10936)
                     /locus_tag="VQ497_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2517872.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
BASE COUNT         3275 a         2506 c         2075 g         3536 t
ORIGIN      
        1 aaaaaaacag aactagtctg aactagtcct gtctactttt acccaatcac acttccattt
       61 ggtacagctg gatcaactgt gagaagggtt aatttgccat catattcagc tgatagaatc
      121 ataccctggc tgacatattt tttcatcatt ttacgtggtt tgaggttagc aacgatttga
      181 actttcttgc cgaccaattc ttgttcattt ggatagtatt ttgcaattcc tgaaagaatc
      241 tgacgatctt ctccatcacc agcatccaag cggaattgaa gcaacttatc tgaaccttct
      301 actttagaca cttctttgac ttctgcgaca cggatttcaa ccttgtcaaa gtcttcaaac
      361 ttgatttcat ccttgtttag tttgagctca acttcgtccg gattccattc tttttcgact
      421 gctggtttat tgccttccat ttgttccttg atataggcga tttcttcttc catatttaga
      481 cgtggaaaga taggtgttcc tttggcaact acagtcacat ctgctgggaa gtcagccaaa
      541 ctcaagtttt caagactaga aacttcttcc aaaccaagtt gagtcaaaac tgcacgacta
      601 gtttccatca taaatggttc aatcaagtga gcaactacac gaatgctggc tgccaagtgg
      661 ctcatgacac ttgccaattg gtcacgaaga gcttcatcct tggccaagac ccatggtgcg
      721 gtctcatcga tgtatttatt ggtacgagag atcagagtcc agactgcttc aagcgcacgt
      781 ggatagtcaa ctgcttccat gtgtgtatgg aagtctgcga ttgattgttc tgcaacctca
      841 gcaagaacat gatcaaattc agtcacacct tctacatagg cagggatttg tccatcaaag
      901 tacttattaa tcatggaaac cgtacggtta aggaggttcc caaggtcatt agccaattca
      961 tagttgatac ggccgacata gtcttcagga gtaaaggttc cgtctgaacc aactggaagg
     1021 ttacgcatga ggtagtaacg aagtggatct agtccataac gctctaccaa catttcaggg
     1081 taaacgacat tcccttttga cttagacatt tttccgtctt tcatgacaaa ccaaccatgg
     1141 gcaatcaaac gatcaggtaa tttaacatcc aacatcataa gaaggattgg ccagtagata
     1201 gagtggaagc gaaggatatc ttttcctacc atatggaaga ctgttccatt ccagaacttg
     1261 tcaaagttac catgttcgtc ttgagcgtag ccaagagctg tcgcatagtt aagaagggca
     1321 tcaatccaaa cgtagacaac gtgttttgga tttgatggga caggcactcc ccatgtaaag
     1381 gttgtacgag ataccgccaa atcttccaag cctggctcga tgaagttgcg tagcatttca
     1441 ttaaggcgac catctggcgt gataaattca ggatgagctt tgaaaaattc gaccaaacgg
     1501 tcttggtatt tgctaaggcg aaggaagtat gattcttcag aaacccattc aacctcatga
     1561 cctgatggag caataccacc agtcacattt ccagcttcat cacggaaaac ttctgccagc
     1621 tggctttctg taaagaattc ttcgtctgat actgaatacc aaccagagta ttcacccaag
     1681 tagatatcat cttgagcaag taagcgttca aagacctgtg cgacaacttt ttcatggtag
     1741 tcatcggttg tacggataaa tttatcgtat gagatatcta gtaattgcca gagttcttta
     1801 actccaaccg ccattccatc aacataggct tgaggtgtaa taccagcttc ttccgctttc
     1861 tgctggattt tctgaccatg ttcatcaaga cctgtcagat aaaatacatc gtagcccatc
     1921 aggcgtttgt aacgtgctag gacatcacat gcgatagttg tgtaggcaga accgatatga
     1981 agtttcccag atggatagta aatcggcgtt gtaatataaa aatttttttc agacataatt
     2041 tttcctttcc aggcaaatga aacctgtttt tctaacactt cattatatca catttttaat
     2101 gaatttcgat agggaaatcc atacaaaaac aagatagacg agtgtccatc ttgttgatct
     2161 cattcataac gaagggcttc aattggatca agtttcgatg ccttgttggc tggcaagact
     2221 ccaaaaatca taccaacact agccgaaact gcaagactaa atagggcaac tgggattgat
     2281 actccaactt ctataccttc tattaaacct tgcagtaaca aacctgctaa ggcagttaaa
     2341 ccacttgcaa ttgtcaagcc aattaagcca cctaacaagg tcaaaatcat ggattcaatc
     2401 aaaaactgaa ttaaaatatt ggcacgtgtt gcacccaaag ctttacgaag accaatctca
     2461 cgagtgcgct ctgtcaccga aaccagcatg atgttcatga caccagttcc tccaacaaag
     2521 agagaaatcc ctgcgatgga actaataatc gtcgtcataa aactaaacga ttgttgaatt
     2581 tctgcaaata caacggactc atctgccacc tggtattctc cctgttgtaa gcctgcaagc
     2641 tctgtcattt ttcgtgccag ttctggaccc agagttgggg ttaaactggt atcattcact
     2701 cgaaagacaa tactagctat ttcatctata ttaaaattcg cagcaaggga gatattggta
     2761 gtaataggca agccaccaaa cccatatatt tttgactttt tagcctccgg actagtataa
     2821 accccaatga cccggtaact aaatccattg acttctacaa ccttgttaat agcctcttga
     2881 ggagattcaa ataaactaat ggacaattcc tcatctagca aaataacact tgcaaactct
     2941 ttgaaatctt gctctctcag actacgacct gcaataattt cattcttaac agcgtccatg
     3001 taagttctgt ttccacctgt caaattagca ttctcaacct ttttatcttg ataggtcaag
     3061 atggcattcg ttgaattggt tacatagtaa ctatccactc ccttcagttt agctgcctct
     3121 tgaacccagg attcttgcgg ttttggcggt tcaacaggaa cttcctcttc ctttccagaa
     3181 accgtaaaag ctgattgttt ctgagtaaaa gacccatctt tacttttttt aggagagaaa
     3241 aagacgctaa tatttttctg agatttagtc atatctttat tgacttgacg agatagggaa
     3301 tcacccaaag ccataatcac aacaactgat gaaacaccga taataatccc aatcatagta
     3361 agcaaagaac gcatcttgtg agccatgata gatgaaaagg caaatttcag attctgcatc
     3421 ttagttttcc tcctttccta actgagcact gtcagacgaa atgaccccat cccgaatgac
     3481 aatctgacgt ttggcatagg cagcaatctc aggctcatgc gttaccatga taatggtttt
     3541 tccttcttta ttcaaatcaa ccaataattg cataatttgg ttacctgttt tggtatccaa
     3601 ggctcctgtc ggttcatccg ctaggataat agaaggattg tttaccaagg cacgcgcaat
     3661 ggctacacgt tgcttttgac caccagataa ttctgaaggt aaatggtgac tacgttctgt
     3721 caattcaacc ttgtctaaat attcctcagc caacttgcga cgttttgaag acgaaactcc
     3781 tgcgtaaatc aagggcaatt ctacattttg cagagcattg agcttcgata gaagaaagaa
     3841 ctgctgaaag acaaaaccga tttgttggtt acggacctta gctagttgtt tttcaccaag
     3901 cccagccact tcttgacctt caagataata ttctccactg gttggtgtat ccaacatgcc
     3961 aatcgtattc atcagagtgg acttaccaga cccagatggt cccatgatgg ctacaaattc
     4021 accctcattc acttctagat tgatattttt gagaacctgc agttcttggt caccattacg
     4081 gtaacttctg aagatatttt ttagactaat tagttgcttc atcagccttc acctcttttc
     4141 cttcttccaa ggaagatgtt ggattactga tgaccttagc accgttcgtt aaaccagaag
     4201 tgatttcttg attttctgcg tcagcatttc ccaatgaaac ctcaactttt ttagcctttt
     4261 gttgttcatc cacaatccag acataatttt tactatcatc cattactaga ctgctaacag
     4321 gaataagaat agccttagtt ttgcttttaa cctcaatgtt gacagaaaaa ccttgtttca
     4381 aatcaccaac ctcgcctgtc acatcaatag tataagggta tttagaacct gtattattcc
     4441 cggctgctgg actagctgct tcaccattgt ttttaggata gtcagaaata tagcttaatt
     4501 tcccagtcca ttttttatca ggatacactt tagaagtaaa gcttacttct tgacctacag
     4561 aaaggttggc tagattgtac tcagacaatt ctcccttgac ttgtaaattt tcattgctga
     4621 caatatgaac cataacttga ctcgcccctg ttggagattt agaaacattg ctattgactt
     4681 cgactacagt tccctctagg gtactgagaa cagttgttgc atccaattga ctttgagcct
     4741 tgcttaattg cgctgcagca tctgcacgcg catcacgggc atcacccaat tgagcatcaa
     4801 tagaagcaac agaatttcca gccactggag ttgggctttg caccgttgca tcttctcctc
     4861 ctactggcgc tggtaactgt ggagcctgag ctgaagcggc ttcatttcgt gcttgattga
     4921 gttcattgat atgacgatct gccttagcta ctgctcgact agctgaatca taggccgcct
     4981 gcgcttctga actactgtac ttgactaaag cctgcccttc gctgacctta tcgcccacag
     5041 aaacaaggat ttcatctaaa tcacccttac tagcatcaaa ataaacatat tgttcatttt
     5101 ttgctgttac tgtccctgac aataaaacag aggatgccac gcttccttcc ttggcaacaa
     5161 caagatgagt aggctcatct tttacagcag tctgaaaagg ttgtctaaaa agtaaaatcc
     5221 ccccggcacc caatacaact acactcgcag caccgattgc tgcatacagt tgccactttt
     5281 tagctttacc attctttttc ttcataatga aactcctttt cttttttaca atactttgct
     5341 attataccaa atttccctcc agcaaacaat gcagttcaga tggaaataat ggcagttcag
     5401 gattaaacaa tcgttcggaa ttttgctttt ctattgtact agatatataa tacactatct
     5461 tgacttattt ggcaagcttt tttgcaattt tttgtgcgta gcagattttt gcaatcaaat
     5521 caaaaaaaga ataaaatata actatcaaaa aagacactct actatttaaa agattacaaa
     5581 ggagttttca atgagagaat atgatatcat tgctatcggt ggaggtagcg gaggaatcgc
     5641 taccatgaac cgtgctggtg aacatggagc caaagcagcc gttattgagg aaaagaaatt
     5701 aggtggaacc tgtgtcaacg tcggttgtgt tcctaaaaaa atcatgtggt acggggcgca
     5761 aatcgctgag actttccatc aatttggaga agactacggc tttaagacta ctgatcttaa
     5821 ctttgacttt gcaaccctac gtcgcaatcg tgaagcctac attgatcgcg ctcgttcttc
     5881 ttatgatggt agttttaaac gcaacggtgt agacttgatt gaaggtcatg ctgaatttgt
     5941 agattctcat actgtaagcg taaatggtga actgattcgt gctaaacata tcgtgattgc
     6001 tacaggtgcc catccaagta ttcctaatat tcctggtgct gagctaggtg gctcttctga
     6061 tgatgtattt gcctgggaag aacttccaga gtcaattgcc attctaggcg ctggttatat
     6121 cgccgttgaa ttagctggcg tactccacac ttttggtgtc aagacagatc tctttgttcg
     6181 ccgcgatcgt cctttacgtg gttttgattc ctacatcgtt gaaggtttgg tcaaggaaat
     6241 ggaaagaaca aacttaccac ttcacactca caaagtccct gtcaagttag aaaaaactac
     6301 tgacggcatt accattcatt tcgaagatgg tactagtcac acagctagcc aagttatctg
     6361 ggctacaggt cgccgtccaa acgttaaggg cttgcaactt gaaaaagctg gagtgactct
     6421 gaacgaacgt ggctttatcc aagtggatga ataccaaaat actgttgttg agggaatcta
     6481 tgctctaggt gatgtaacgg gcgagaaaga actgactcca gttgcaatca aggccggacg
     6541 taccctatct gaacgtctct ttaacggaaa aactactgca aaaatggatt actcaactat
     6601 tccaactgtt gtcttttcac accctgctat cggaactgtt ggtttgacag aagagcaagc
     6661 tattaaagaa tacggtcaag accaaatcaa ggtttataaa tcaagctttg catctatgta
     6721 ctctgcttgc actcgcaacc gtcaagaatc ccgtttcaaa ctcataacag ctggttcaga
     6781 agaaaaagtt gtcggacttc atggaattgg ctacggcgtt gatgaaatga ttcagggatt
     6841 tgccgttgct atcaaaatgg gagcaaccaa ggctgacttt gatgcaactg tggcaattca
     6901 cccaactgca tctgaagaat ttgtaaccat gcgttaatcg aaacaaaaga agctgatata
     6961 aatatgtctg cttctttttt aatgattttc gaatcaacaa aaaaattatc caacttgact
     7021 agtcacgaaa atcggctgtt acagtttttg ttaccttgtt tttaaaaaat ggttgacatc
     7081 gactcctata tgagtataat agttatcata cattagaaaa gaggttaact attttgaaaa
     7141 aagctcacgt ttatgctatc cctgctattg gggctgctct cattgctgct ttggcacaaa
     7201 tcagtcttcc aattggacct gttcccttca ctctgcaaaa ctttgcaatc ggcttgattg
     7261 ctactgtctt tagaccgaga gaggctgtac tttctgctgg actctatctt cttctaggtg
     7321 ctatcggtct tcctgtcttt gcaggaggtg gagctggttt tcaggcttta gttggcccta
     7381 ctgcaggcta tctttggttt tatctcgttt actctggact tacttcctct ctaaccaaca
     7441 gcaagagtgg tgttgttaag atttttcttg caaacctctt gggtgatgcc cttgtctttg
     7501 tcggcgggat tctcagcttg catttcctag ctggaatggc atttgaaaaa gctcttgctg
     7561 tgggggttct tccctttatc attccagacc ttggcaaact tctggctatt agttttatta
     7621 gccgtcccct acttcaacgc cttaaaaatc aggcttactt tactaactaa aaaaggatat
     7681 cgagttatca tgactcaata tccttttctt ttattttgaa aacttatact caatgaaaat
     7741 caaagagcaa actagaaagc tagccgcagg ctgctcaaaa cactgttttg aggttgtgga
     7801 tgaaactgac gaagtcagta accatacata cggcaaggca aagctgacgc ggtttgaaga
     7861 gattttcgaa gagtattagt tgcctttaaa ggcatccacc atcgtttgaa attcttcatt
     7921 tgagagagta atccctttgc ccattttagt atggtctgga ctccaagcac gaatatcaaa
     7981 ctttgcaggg gcaccattaa agctcacacg gttaatttcc ttggtccaac ctttttcgtt
     8041 ttcagaaaga gtcaacaagt gctcttcgat ttcaaatgta aattctgcca ttttcttctc
     8101 cttttttagt ttcattagtt tattcgtaaa atcttgtaga ttttaggaaa attttatata
     8161 atattgatat aaaagaaggg aggccaatat gagacataaa ttccagcaag ttctaaataa
     8221 aatacatgat tttttaaatg gatatgacca acctgaccag actgaaacca actcccttac
     8281 agccactatt gaagaggcta tccagaaaca aaccgctgtt caccttatct tgtctgagac
     8341 aagttttaca ggtgatatca tcaaatatga tcagcaaggc cagcaaatta tcgtgaaaaa
     8401 ttttgccaaa aatgtgagcc ggattatccg tataagcgat attcaacgcc tgcgatttgt
     8461 cccctcaact gtccaaacag cccaaaaaaa tagatttaag aaagagtgag atgtagttgc
     8521 ttcatcccac tcttttttct tagcgaattt gttcaaaatg taaatgaact gcaatatgat
     8581 ctccataacc acttctttcc aagtcacgtt gtaaacgata ggaaatgtag tgttctgcaa
     8641 tggtaatgta acctgcgccc aataaacgat gttcaaccat agattgaatc atactgatag
     8701 tcgcacgttc caccttggct tcttgtaaat ccaaaactac cttcttagtg acttgagcaa
     8761 gattttgacg caaatcatct gtcaaaacat aaacagtttg ggctgccttc aagatggctt
     8821 ggtaaatctt atctggatta aattcagcaa tttcgccatt acgtttgatt acttgcatag
     8881 gtttctcctt tattctttgt tttctttgat ttctgccagc attttttctt cttctactgt
     8941 cagttgataa tgttcaagta aatccggtct gcgctcgtag gttttcttta aactctcgta
     9001 caatcgccac tgacgaatct tttcatggtg gccactcatc aatacatctg gcacgaccat
     9061 gcctcgataa tcatagggac gtgtgtactg aggatattct aaaagacctg aagaaaaact
     9121 atcatcttgg tggctagact ccttgccaat cacttctgga atcaggcgaa ctgtagcatc
     9181 aatcatggtc atagctgcca attctccacc agtgaggaca tagtcgccta gggaaatctc
     9241 atctgttacc aaggtcttaa tgcgctcatc ataaccctca tagtgcccac agataaagat
     9301 tagctcttcc tcttgagcca aatcttcagc ataagcctga tcaaactgct ttccagcagg
     9361 atcgaggaga ataacgcgcg gatttttctt ttcaatagca tcaaaggaat cgaaaatagg
     9421 ttgtgctctg agcaacatgc cctgaccgcc tccgtagggc tcatcatcta catgacgggc
     9481 cttttcagca ttttctcgaa aattatgata ctggatatcc aagagccctt tttctcgagc
     9541 ctttccaacg attgagtgct ccagtggaga aaacatctct ggaaagaggg ttaaaatatc
     9601 aatcttcatc gtctaaccct tctaagattt ccacatcgac ccgtttattt ggaatatcaa
     9661 cattgagaac cactggtggg atataaggta aaagcaaatc acgtttgcct tttcgtttga
     9721 ccacccagac atcattagca cctggttgca ggatttcctt gatggttcca accaagctat
     9781 caccctcata gacttccaaa ccgataatct cgtgatagta aaattcacca tcgtctaggt
     9841 cattcaaatc ttcctcagcg accttgagac tatatctctt gtacttttcg atagtattga
     9901 tatggtacat atctttgaat ttaataatgt caaagttctt ctgtttacgg tggctagcga
     9961 tggtcactgt ttggacaaac tgatcttttt catcaaacaa agccagctca gctccttttt
    10021 taaaccgttc ttctgcaaaa tccgtcacag acaagactcg catctcaccc tgtaatccct
    10081 gcgtattaac gattttccca acattaaagt agttcatctt gtctcctgta atctcctttt
    10141 ttccatctta ttctgacaat tctcgaataa tagccgcaat tttttccgat tctgaccatt
    10201 gtaaataatg gtgattccct cctaaaatga gtttagtatt ggaagtccaa tattctgatt
    10261 ctctgtactc tttttctcta taaggctgac aaaaaacaaa tacaggaata tgagcttcta
    10321 tagatacatc ctcaaaatct tcctcagtaa tctctccaga tatctgaaat tctggatctt
    10381 gattttccaa ctctaagcct ttttcttgca ttaattccca gattttttta ttcgtttcag
    10441 gactaaatgt tgcttgagtt aagttcttaa aataaagttc aggaccacac tcgtcaatca
    10501 gcctcatctg ctcttccatt tctggataag gattttctga aaaatcagca aacatgactt
    10561 ttttagttgt cggttcaatt gctactaaag tctgacgctt aattggtttc tcgagtaatt
    10621 tgcaagctaa aattccactc caactatgtg cacaaagtat atattcagaa attcctaatt
    10681 cttcaagtac ttcataaacc gcatctgcaa gattatctag attttttcca gcttggtcat
    10741 gaatcggact cctacctgtg ttcggaaaat caattgtcaa ataaccaatt gtaggaggag
    10801 gtttttcaag tataagtgaa aaattttcat aacttggtag caaacctgct ccgtttagac
    10861 aaactagcac tttcttttgc ttttgataag taacagagag gctaccaatt tctgtagata
    10921 cttcaaacct cttcataaag aaatccactc attctatata atgaattatt aaaaatcctt
    10981 atcttttatt ttatcacgtt ccaaggattt tctcaagttg gaggaagggg acaacatctc
    11041 tactttccct tcaaaaatcc ttccaaatca tgtttatgtt ggtaattaac ggctgcggtt
    11101 ttgtctttct caaagacagt cttggtaagg tcaatatgat taatagctac gattgcgacg
    11161 gtgtagtaaa tgatatcagc cagttctctg gcaagttcct cgttcgaatc ctatcccttc
    11221 ttttcgacca gagcgcctat tcaaaacctc ggctacttct ccgacttcct ccactaactt
    11281 cataaagaga ccttcatcag tccgagactg ctgttaatgt tcgattaagt agtcttggaa
    11341 ttgcctaaac gttcaatctt ttatagtata ttgaaactag aatagtacac ct
//
LOCUS       DAVBCN010000061        11086 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVBCN010000061 DAVBCN010000000
VERSION     DAVBCN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11086
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.061"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(6..890)
                     /locus_tag="VQ497_001579"
     CDS             complement(6..890)
                     /locus_tag="VQ497_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HET2517873.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDMIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(894..1280)
                     /locus_tag="VQ497_001580"
     CDS             complement(894..1280)
                     /locus_tag="VQ497_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HET2517874.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLISLMLEEQILTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(1273..2616)
                     /locus_tag="VQ497_001581"
     CDS             complement(1273..2616)
                     /locus_tag="VQ497_001581"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET2517875.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYEVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEGLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(2698..3582)
                     /locus_tag="VQ497_001582"
     CDS             complement(2698..3582)
                     /locus_tag="VQ497_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2517876.1"
                     /translation="MIQVRNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHIEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(3594..4211)
                     /gene="cysE"
                     /locus_tag="VQ497_001583"
     CDS             complement(3594..4211)
                     /gene="cysE"
                     /locus_tag="VQ497_001583"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HET2517877.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFIW
                     KHGFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(4227..6440)
                     /gene="pnp"
                     /locus_tag="VQ497_001584"
     CDS             complement(4227..6440)
                     /gene="pnp"
                     /locus_tag="VQ497_001584"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET2517878.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDDQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRLELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            complement(7125..7991)
                     /gene="metF"
                     /locus_tag="VQ497_001585"
     CDS             complement(7125..7991)
                     /gene="metF"
                     /locus_tag="VQ497_001585"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HET2517879.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(8055..10304)
                     /gene="metE"
                     /locus_tag="VQ497_001586"
     CDS             complement(8055..10304)
                     /gene="metE"
                     /locus_tag="VQ497_001586"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HET2517880.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVASDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(10565..10831)
                     /locus_tag="VQ497_001587"
     CDS             complement(10565..10831)
                     /locus_tag="VQ497_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2517881.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGVSQPVIARMETGESSLQLDTVLKVLTSLGKTLAVVPLEQGKS"
     gene            complement(10915..11086)
                     /locus_tag="VQ497_001588"
                     /pseudo
     CDS             complement(10915..11086)
                     /locus_tag="VQ497_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         3096 a         2540 c         2193 g         3257 t
ORIGIN      
        1 cttctttaca aaccaactat agttgacaaa gaacctaaaa tcaattgata acacaaggtc
       61 aggtcggtca actctttcaa ctgaagccct gtcaactctt cccatttatc aatcttgtat
      121 tggagagaat tgcggtgcag atagagttgc tgggctgttt tagtgagaac agcactattt
      181 tcccaaagag agagaatgat ttcctgaatc tgatcttgat ccaaaatcat ctggtgtaga
      241 cattctttaa ttggcttcaa gtccacgagt ctttctccca tactccaaag atagagctga
      301 gaaaaagtat gaacaccttg gtgaccctga cgccaccatg tcttgaacaa atcccgctca
      361 gctttgatta agtctgatag ggcttgatgt cctgtctgag accaaacctg acccaacatg
      421 atagaaagac gaagtccaaa gtcatactca actgcttcaa tcatatcact taaaatatct
      481 cttacagaag tgtatttgtc ttgttgaagc acgaaaacat aatcctgagc tccgacctgt
      541 aacactgtct gacaattcgg aaaaagagtc cgcatcatat ctagccaaga agccagattt
      601 tcctgctgaa aataagaaag atggcaataa accaactgaa tctttttaaa aacttgcggt
      661 gcctgtccct tgccttcaac cagataggaa taccaagggt ttagcgaacg agcctgctcc
      721 tgctgggtca aaagggcaac caactgcttt tcacgctcgc tgagcccagc ttcctccagc
      781 aaaatccact gctgagaagc taaaggaagc gtgagatagc cctctttctc tactggttgg
      841 tctgaaatcc gagcctcagg aaaccagtct tgtagttctt ttgccctcat gttctagccc
      901 tccacttttt ggatgcacca tgaaaccaaa ctctcaagac gttccagatt ctcagtcata
      961 tggagatagc ccataactgc ttcaaatccc gtggacatac gataagtcac gacatctgca
     1021 tttttagcct ttgtgtgact attggtattg cggccacgtt tgtagatttc ttcttctttt
     1081 tccgttagga tctgctcctc caacatgaga gaaatcaggc gagcttgagc cttggctgac
     1141 acgtacttgg tcgcctcttg gtggagttta ttgggcttgg tcataccttt gaggatgagg
     1201 tggcggcgaa tatacataga atacaccgca tctccctcaa aggctagcgc aatcccgtta
     1261 atgagattga catcaatcac gtgtccacct cactccatcc ttggtatcaa ggagcttaat
     1321 tccttgagta accaattggt cacggatttg gtctgctgtc gcaaagtcac gattggcacg
     1381 cgcctcttgg cgtttttgaa tcaagtcttc aatctctgca tccaaaactt cctcaacaaa
     1441 gacaattcca aaaatttcta acatatctgc aagagcttgc ttgacacttg catcatagtt
     1501 ccctgagttg atccatttgg ccatttcaaa gacaactgtg ataccgttgg cagcattaaa
     1561 atcttcatcc atagctgcta caaacttatc tttaaagttt tgtaactctt gggcatccac
     1621 gtttcctgta aatggttgtt cgtaagtatt cttcagatac ttgagattgg tctcggcatc
     1681 gcgaactgcc ttttccgtga agttgatagg cttacggtag tgctgggtcg caaagaagaa
     1741 acgaagtact tgcccatcaa gagttttaag ggcatcgtgt accgtaatga agttacccaa
     1801 ggacttagac attttgacat tgtcgatatt gacaaagcca ttgtgcatcc agtagttagc
     1861 aaaagccttg cctgttttag cttcagattg ggcaatttca ttggtgtggt gtggaaactc
     1921 taggtcagct ccaccaccgt gaatatcaat ggtatcacct aaaatctctg tcgacatgac
     1981 tgaacactca atatgccaac ccggacgtcc aggtccccaa ggactatccc aagaaatctc
     2041 acctggtttg gaagatttcc atagagcaaa gtctacagga ttttccttac gagccgtttc
     2101 ttcatcggta cgacctgaag cacctagctc caaatcttcc aaggttttat tagccaattt
     2161 agcatagttg tgggattttt ctacacggaa atagacatcc ccttgactct cataggcaaa
     2221 gcctttctcg atcaagcctt ccacaaaacg gatgatgtct gccataaact ccactacacg
     2281 cggatggcga gtcgcaggtt tcacgcccaa ggccgtcaca tcctcacgaa aggcagcgat
     2341 gtacttatcc gcaacctcct gaggcgtgat gccttcttcc ctggcacggt tgataatctt
     2401 atcatccaca tccgtaaaat tggaaatata ggcaacctcg tacccacgat actcaaaata
     2461 gcgacgaatg gtatcaaaag ctaccgttga acgagcattc cctacatgaa tatagttgta
     2521 caccgttggc ccacaaacat acatcttgat cttgccgtcc tcaatcggga caaattctcg
     2581 caaatcacga gacatggtgt catagatttt aatcataaat cataatcaag aaagctgaaa
     2641 tccaagaaca attagtttca tcactaaaag ttcaagtaaa tttcagtccg aatatctcta
     2701 cacttcggaa tcccttgctc ctttctcatt cagataaacc acctgagtct gtttgacaaa
     2761 gccaattttt tcatacaaac gtttggcacc tacattgcta tcttccactg caatctgaaa
     2821 ttccttgtca ttttgctcaa ttagttggtt gacgagggat tttgctaagt agcttccata
     2881 gcctttccca cgttcaggtt ccgatattgc taaaccgtag aagtaattcg tattagtcga
     2941 taaatctacc gtgcaagtcc caataacctg accgtctttt aacaaaatat atagtcggct
     3001 ttctggatcc ttcagagctt cagcgacata tctatccaca acttctctcg attcatgttc
     3061 ctctgaaaat gcctgaaatt ttaattgact aatttgatcc tgatacgaac tatctgctaa
     3121 caaaacttca atatgggaaa catttgctaa cggataaggt cttctatcct tacctaacca
     3181 agtttctgtc tcttcatcct cgattagtcc ccagttactg gcaaagtcag gatgattctc
     3241 taaaaaaata cgttctgtct gaaaagtgac tgatcgaatg gggaaagaag ctgtttctct
     3301 ctcaaaacta gtaaacaatg cacgcgcaat cccctgacgg cgatgacctg gatgaaccag
     3361 tatcgtcact tctacatctt ggtcatctgc atagacagtt aataaaccaa caagttcgcc
     3421 tttttcataa taaaggaaaa aggcgggcat gtttgggtca aaattaagca tgttagagag
     3481 atagggatcg cgataggtac cgtcatagtt ttggcaacag ttaattactt ttttcgcctc
     3541 agatagctcc tcttggctta acttgtttct tacttgaatc atataggtat cctctacaaa
     3601 ccagacgatc tgtgactggc atctttagcc tgctcgagtt tattgacata atactctcgt
     3661 ttttcttcga cttcgtgaat gacaggctca tctttcttac catgaagacg gacaatcttg
     3721 gccggaatac cgacaaccgt cacatcacta ggtacatctg ctacgacaac tgctgcagca
     3781 ccgaccttgg cattttcacc aatttccaca ggtccgataa cttgggcatg ggctgatatg
     3841 agggctccct ttcgtacagt cggatggcgt ttgccacagt ctttccctgt tcccccaaga
     3901 gtcactccgt gatagagaag aacgcctttt tcaacaatcg ctgtctctcc aatcaccaga
     3961 ccagaaccat ggtcaataaa gacacctgaa tcaatctggg ctcctggatg aatctcaatc
     4021 tgagtccaaa agcgccaaaa ctgactgtgc atacgagcta agagtttgaa gccgtgcttc
     4081 cagataaaat gcgagagacg gtgggccgcc aaggccttga cacctggata agtcagcaaa
     4141 acctccaaag tggtgcgggc cgctggatca ttttctttta cgatatcaat ggtttcgcgc
     4201 caccacccca tacatttctc cttttcttat tctgaatctt ttggtgtttc tgtaaattct
     4261 ttcttaggtt tgtgatcctt gtgatgacgt gggcggtgag gacgctcaga cttttcacct
     4321 ttttcatcat gctcaggttt tggcggacga ggtagaagag ccttcataga ggcatcgata
     4381 cggccttttt catcaatctt gataacctta acatcaactt catccccgat ttctaccaaa
     4441 tcctctacac gattggtacg agtccaagcc atctcagaga tatgaacaag ggcatctgtc
     4501 ttatcaaaga ggttaacaaa ggcaccaaat ttctcgatac gaacgacttt agcacggtaa
     4561 acttcatcca ctttggcttc acgaaccaaa ccagcaataa tttctttggc acggttaata
     4621 gcatcttggt cactagagta gatagacaca tttccttctt cgtctatatc aatcttaaca
     4681 cctgtttcag cgataatctt gtcgatggtt tctccaccct taccgatgac aatcttaatc
     4741 ttgtccacat caatcttgat cgtatcaatt ttcggagcag ttggagccaa ttctagacga
     4801 acttctggaa tggttgcttc aatgacatca aggatttcaa aacgcgcttt cttggcttga
     4861 gcaagagcct ccgtcaagat ttctgcagta atcccttgaa tcttgatatc catttgaagg
     4921 gctgtaatcc catcacgagt acctgcaacc ttgaagtcca tatctccaaa gtgatcttcc
     4981 aaaccttgga tatctgtcaa tactgtgtag ttatttccat ctgagataag tcccatagca
     5041 ataccagcta ctggcgcctt gattggcaca ccaccagcca taagggcaag agttcccgca
     5101 cagatagaag cttgagatga agaaccgttt gattccaaaa cttctgctac tagacggata
     5161 gcgtatggga attcttccaa gcttggcaag acttgagcaa gagcacgctc accaagggca
     5221 ccgtgaccga tttcacgacg acctggcgca ccgtaacgac ctgtttcccc tacagaatat
     5281 tgagggaagt tatagtggtg cataaagcgt ttcttgtact ctggatccaa accatcaatg
     5341 atttgagttt ctcccatcgg agccaaggtc aagactgaaa gagcttgagt ttgcccacga
     5401 gtaaagagac ctgaaccatg tacacgagga aggaagtcaa caaccgcatc caaaggacgg
     5461 atttcatcga ccttacgacc atcaggacgc accttgtctt ctgtaattaa acgtcgcact
     5521 tctgcgtgtt ccatttgttc caagatttca gccacatcac gcataatacg gtcaaattct
     5581 tcgtggtccg catatttttc ttcgtaaacg gcagtcactt ggtctttcac tgcttgagtt
     5641 gcagcttcac gggccaattt ctcttctact tgaactgcct tttggaggtc actgttgtag
     5701 gctgcaatga tttcagcttg caattcagca tccacgtgaa gcaattccac ttctgctttt
     5761 tctttaccga cagcagcaac gatttcttct tggaaggcaa tcaattcttt gacagcttcg
     5821 tgccctttaa gaagcgcttc caacatgatt tcttctgaca attctttggc accagactct
     5881 accatgttga tagcgtgctt agttccagct actgtcaatt caagaagaga ttgctctgct
     5941 tgttcttgac ttgggttgat gatgatttgg tcatctacat atcccacttg taccccagca
     6001 attggtccgt caaatggaat atctgaaata gacagtgcca aagatgaacc aaacatagca
     6061 gccattggtg cagatgcatt ttcatcataa gaaagcactg tattgatgac ttggacttca
     6121 ttacggaaac cttccgcaaa cataggacga atcggacggt caatcaaacg cgctgtcaag
     6181 gtcgcatctg ttgaaggacg tccttcacgt ttcataaagc caccaggaaa cttcccagcc
     6241 gcatacattt tttcttcgta gttgacttgg agtgggaaaa aatccccagt tgccattttc
     6301 ttagacataa cggcagcagt caagacagtt gactcaccgt aacgtacgac aacagagcca
     6361 tttgcttgct tagcaacctg accagtctct acaattaact cacgacccgc aaaagtcgtt
     6421 tgaaacactt gttttgccat tttaatcccc tttggattga tgaaattata cgccttgcct
     6481 acaaagatca agataccaag gacgtcaaaa gcaaagtaaa aataggaaac tgacgaagtc
     6541 ttcgatgaag acaagacagt ttatcttttt tacacagctt ttcggccgtg ttcaattaca
     6601 caagatattt tggacggttc ggcttgccga acatttctgt agaaaaatag gaaggtgacg
     6661 tcgcactcga cgagtgctag gaagcttatc ttttttccta agaaatgaga ccaaaattca
     6721 agtcatcaag ataccaagcc gtcaagcaac tcaaaggaaa ataggaaatc gaacgacgga
     6781 gcgactgctc ctaggcagat ttatcttttt ccacagagtt gtaggcgtgt tcagttttca
     6841 agatacatca ttagaaaggt ttaatattaa agtatctaaa cctttcacgc taatcgctat
     6901 cgggcgatta gctaaatgct ttactaactc tttcgtcaaa taacatcgat ttgactcact
     6961 cgtgtcgtta aatcttacag tttaaatgca ttgtattatt taataccttc atctttgtat
     7021 caagtacgta cagaatctat tttatcatat ttttcttaaa aagtgaggtc tttaccatta
     7081 aaaaggaacc attcccctca cctgagaaga atggtttgct tttattatcc tagagactgg
     7141 tgattaaaca aggcatgggt tgcttgatgg atgtattttg ctgtatcagc attattcatc
     7201 gtatagagat gcacaccggc aacatcctga gttaccaagt ccacgatttg gtccactgca
     7261 taggcaagtc ctgctgctct gagcgactca gggtcatgct catacttgtc taagatggct
     7321 ttaaatttgc gtggaagatg gatattctca caagtcttca agagtcggag agcctgattt
     7381 cgattcagaa ttggcataat tcctgcatga ataggaacat caatcccagc caagatacac
     7441 ttgtcctgaa aatcatagaa gcgctcattg tcaaagaaga gctgagttac gaggctcgaa
     7501 cagcctgcat ccactttctt cttaagattt tgaatatctg aaatctgatt tggcgaatct
     7561 ggatgccctt ctggatagca agctccaata atatcaaagt gaggggcttg ttccttgata
     7621 aactcaatca agtcggttgc atagcggaaa tccttttgtg gttctacgtc tggaataata
     7681 tccccacgaa gagccaagat tttctgaacc ccaactttgt ccaagtcagc aatagtttca
     7741 gcaaccttgt ccttagttag ataaatagct ggcaagtggg caatggtcgg aatcgccaag
     7801 tcattttgga taaagtcagc caaacgaacc gtcgtttcct tgatattaaa tttattattg
     7861 ctggcagtta cactgataaa atggggagcc aactcctgca tatcctgcaa ggctgaaata
     7921 atgttaccat tacccacggc tgggtttgga gggaacactt caaatgagag tgacggtgtt
     7981 tggcgtgaca tatgtaataa ccttttctag ttgatttctt tttgaacaac cactgtatgg
     8041 agagaaatcc aatcttacaa tttctcacgc gcagctttag ctgcttcaac aaggcggatc
     8101 aagctttctt ttgtttctgg gataccacgt gttttcaaac cacagtcagg gttgatccaa
     8161 actttcttgc ttggcacttt agcaaggatg gcttcgattg tgttgtcgat ttcgccttca
     8221 tttggtacac gaggtgagtg gatatcgtaa accccaggtc ccacttctgt ttggaagttt
     8281 ttcgctttga gttcgtccaa gatttcaagg tttgaacggc tggcttcaaa ggaaataacg
     8341 tctgcatcca tgttatcgat agctgggatg atatctgtaa attctgagta acacatgtga
     8401 gtgtggattt gtgtgtctga cgctactgtt gagtgtacca agcggaaggc aggaattgcc
     8461 cagtcaaggt aatcttcgta ccagtcacta cgacggagtg gcaatttttc acgaagagca
     8521 gcctcatcaa tttggatgat tttcacacca gcagcttcaa ggtcaagcac ttcatccttg
     8581 atagcaaggg cgatttgaag agtagaatcc ttgatagaga tgtcttcacg tgggaatgac
     8641 cagttaagga tggtaacagg tccagtcaac atacctttaa caggtttgtt tgtacgactt
     8701 tgtgcatagc tagaccattt aacagtgata gggttaagac gagtgacatc accccagatg
     8761 attggtggtt ttaccccacg cataccgtat gattgtaccc atccattttt agagaagagg
     8821 tatcctgaca agttttgacc gaagtactca accatgtcat tacgctcaaa ttcaccgtga
     8881 acaaggacat caaagtcaat atcttcttgc cacttgatcc attcgtcaat cgtttcagca
     8941 aggaaagcat cgtactcttt ttgagacaat tcacctttac ggtaagccaa acgtttggca
     9001 cgaacttctt ttgtttgagg gaatgaacca atcgttgttg ttggaagagc tggaagtttg
     9061 aaagcttctt cttggatagc ttcacgttct gcaaaggctg gcaaacgagt gtagtctgcg
     9121 tctgtcaagc cagcgatacg cgcacgaagt tcagcatttt caccaacacg ctcagtcgca
     9181 aagagttctt tgttggctgc aagagcttct gaaccttgac cattgcggat agcatccaag
     9241 tcacggattt catccaattt ttcaactgca aaggcaaagt ggttcaagag tgctggttca
     9301 aattcttcat tagcagttgt aaatggcaca tgaagaagtg agcaagagct tgtcaaaacg
     9361 atgttttcag ctggaatttg ctcaagaaca gccaagctct tttcgtagtt gttgcgccag
     9421 atgtttttac cattgacaat acctacatag agagtcttgt cagctgggaa gccaccttta
     9481 acgagttcaa gagttttctt accttcaaca aagtcaagac cgatagcatc tactggtaag
     9541 tttacaaggt cagcgtatac gtcacgaaca tcaccgaaat aagtttgaag caagacttca
     9601 agaccttttt tgtcagccaa gagtttgttg taaaggttca agaagagagc tttttcttca
     9661 gctgtcaagt cttttacaag agccgcttca tccaattgga tgcgagtcgc accaagttca
     9721 gccaatttag caaaaacttc ttggtaagca gccactaagc tatctacgaa gtcgtctgct
     9781 ttcacgcctt cttcaaagtc tgacaattga aggaaagtga agggacctac aagaacagga
     9841 cgagtgttca atccaagttc tttggcttct tggaactcat cgaaaatctt gtgaccagcc
     9901 aattttactt gagtgtcttt ttcaaattta ggaacgatgt agtggtagtt agtgttgaac
     9961 catttcttca ttggaagggc gcgaacgtcc cctttttctc cctggtaacc acgtcccaaa
    10021 gcgaagtagc gctcaaggtc agacaagtcc aagttttgaa cggatgcagg caccacgttg
    10081 aaaaggaaag ccgcatctag gaagttatca tagtgagaaa agtcatttga tggaatttca
    10141 gtgatgcctt tttctttgac aatgttccag tgtttagcac gcaagtcttt tgctgctgct
    10201 aaaagttctt cttctgagat ttcttttcta aagtattttt cagttgtaaa ttttaattca
    10261 cggaattcgc ccaaacgagg gaaaccgatg attgtagttg acatgatgtg tcctccaaaa
    10321 tttgttgttg aaactatctt aacagaaaag aaagcgtctg tataattgta aaaaattagg
    10381 gtttgatata gtttgaaact atatatctgt ttcggacaaa agaaaaagac ttgaagcaaa
    10441 cgtctcaaat cctttgtaat tcttacttta cagctatatt ccaattagaa tactaaaaca
    10501 tgttattagt aattcttata agtgactatg acctgttatt agaaaagact ataactgatt
    10561 ctagtcaact tttcccctgt tcaagtggaa cgactgctag tgtctttcct agactggtta
    10621 gaacttttaa gaccgtatcc aactgaagac tgctctctcc tgtttccatc ctagctatga
    10681 caggttgact cactccactg acttcttcca gctttttctg actgattcct tgttcatacc
    10741 tagcctcaat caactcgctc atgatagcca ctcgcatatc actttcaagg atttcctcct
    10801 tgctaaagag ctcagatgga catccttcca attactccca atagcactat tcttcatcac
    10861 ttaaccctct ttcttttacg tctatgtatt tttaaaagaa tgagcgaatt atgattcgat
    10921 agattgacca gcaggtttaa agttggtgct agcctgtttc ttaagcgctt ttccttttct
    10981 agggtaaagc agatcctgct tgcttaaccc caattttcca cgatgaatcc aatagtaaat
    11041 ggttgaaatt cccacgttaa cccctttagc catcaccatc atttca
//
LOCUS       DAVBCN010000062        11021 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVBCN010000062 DAVBCN010000000
VERSION     DAVBCN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11021
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.062"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(<1..695)
                     /locus_tag="VQ497_001589"
     CDS             complement(<1..695)
                     /locus_tag="VQ497_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET2517882.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMKELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     KGEILEEFSMSAV"
     gene            1319..1501
                     /locus_tag="VQ497_001590"
     CDS             1319..1501
                     /locus_tag="VQ497_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET2517883.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLSFNEKFGTENT"
     gene            1511..1876
                     /locus_tag="VQ497_001591"
     CDS             1511..1876
                     /locus_tag="VQ497_001591"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET2517884.1"
                     /translation="MPNSENTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYYGDSAWEHLRAVTGI
                     DLLSILTDIANKKTGVKSK"
     gene            1873..3063
                     /locus_tag="VQ497_001592"
     CDS             1873..3063
                     /locus_tag="VQ497_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET2517885.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADM
                     FQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERK
                     RNGNSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQ
                     IIKIATDKNDIVLDPFCGSGTTLVASKILNRNYMGIDLSEEAINITQQRLENVIKTSS
                     NLLNKGIEAYRTKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQKKPIPIKIQKNNEC
                     LNESISLLQNAINSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKS
                     QQLL"
     gene            complement(3578..4570)
                     /locus_tag="VQ497_001593"
     CDS             complement(3578..4570)
                     /locus_tag="VQ497_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2517886.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
     gene            4730..6484
                     /locus_tag="VQ497_001594"
     CDS             4730..6484
                     /locus_tag="VQ497_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2517887.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISAVLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIVTPILVLVLGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAEVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAIIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            6468..8189
                     /locus_tag="VQ497_001595"
     CDS             6468..8189
                     /locus_tag="VQ497_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2517888.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERIHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            8200..8829
                     /locus_tag="VQ497_001596"
     CDS             8200..8829
                     /locus_tag="VQ497_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HET2517889.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALIALGAFLGGILGAYIGKALLKKHFSNGLYCVGYFTPCLILWCYLN"
     gene            8730..9287
                     /locus_tag="VQ497_001597"
     CDS             8730..9287
                     /locus_tag="VQ497_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2517890.1"
                     /translation="MYRQGSIEKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIP
                     IVIRMFILPFMAASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKN
                     IKMAMRVRGINFKNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRV
                     KIQLIDFVYVLAVAGLIVGGLIWLK"
     gene            9275..10639
                     /locus_tag="VQ497_001598"
     CDS             9275..10639
                     /locus_tag="VQ497_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2517891.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGSGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDILEIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDESSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLKLDKNELNALSLRDKELSKLKVPYLKEGGEYQIKNLSYKFTDDECLSLK
                     DISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVM
                     QDVNHQLFTDEVFNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAI
                     ASVMCKTSPFIFFDEPSSGIDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADE
                     IFEL"
BASE COUNT         4054 a         1539 c         1864 g         3564 t
ORIGIN      
        1 tcaacggctg acattgaaaa ttcttctaga atctcaccct tcaaactctt acgttctttc
       61 ccaaatggca tatcgtaaag gtcgtatttc caacggcatg actgagaaca cccaccacgg
      121 ttggcatcac gcatactcat gtggtttgaa agagtacaac gtccagagta tgaaatacac
      181 atagctccat ggacaaaggc ttcaatttca acatctgtac gtttgcggat ttcagctaat
      241 tctttcattg aaacctcacg tgctaaaacg acacgagtca agcctagctc tttccagaac
      301 tcaagggttt catagttagt ggcactggct tgggtagaaa ggtggatttc aaggcctggt
      361 gcttcagtcg ctgcaatcat aatcaaggct gggtcagata cgataactgc tgcaatcccg
      421 atatcacgca gtttacgaaa ccactcacca gcaccagctt catttccttc gtgcataacc
      481 atattagccg ctacatagac cttggcacca tacttggccg caaactgcac gccttcttcc
      541 atctgttcga aagtaaagtt tcccgcacgg ctacgaagac cataggcctg accaccgata
      601 aagacagcat ctgctccata ctgaacagct acctttagct tctctaaagt ccctgcaggt
      661 gataaaacct caggacgttt taatgttttt gtcatgattt ctcctatttg caatataaaa
      721 gtttgacatt tttccttctc attttatagt aaataaagga taaaatcaag cctagacgga
      781 ttgttttgac aattctaatc ttttagactt tgcataataa ctattaaaca gtcgaaaatt
      841 accccagttt taacaagtag tcaaaaagtc taaaaaagta aaaaccagta tcccaagata
      901 ctgactcgta aaaatatata aattagtcct tagatgacta ctcccactct gttgctccta
      961 taatggaaaa ggattgatac agtctagaat atcgcttttt tatttattat tctgatataa
     1021 aactgttaga attgattttt tgttttatac ccaaatatga atagagacgt agctaataag
     1081 ttgaataaca tatttggaaa aatgagaaat agcaatatta ctagaacttt tagaaacaat
     1141 caatatttaa aaagaaaaaa gctcaaagaa tttattaaac catgttataa tagatatagc
     1201 tataatttta tttataacaa gaggatagaa atgaccgaat tagaaagaaa aaatcgaaaa
     1261 attagctaag aaatattctg ataacttaaa catcaaagtt caagagagag ttcgtgaaat
     1321 ggcaaatgat aataagagcc attatttgat atacagagtt ttaggtattt catttgaaga
     1381 aggagaaaat atcgatttgt atcaaaataa aggtcgtttt ttatacaaat atgctggttc
     1441 atttttagaa gaagctgcag tactatcctt taacgaaaaa tttggtacag aaaatactta
     1501 aaaagttaac attcctaatt ctgaaaatac aaaacctaag acttttgaaa ttgattgttt
     1561 agtcggagaa aaacacgcat acgaaataaa atggtgggat gcaactacag atggagacca
     1621 tataactaaa gaacacacta gaataaaagt tattcataac aaaggatata taccaattcg
     1681 gttaatgttc tactatccaa atagaactca agctataaaa attcagcaaa ctttagaaac
     1741 attgtataac ggtattggag ggaaatatta ttatggagat tctgcctggg aacatttaag
     1801 agcagtgacc ggtattgatt tacttagtat tctaacagat attgcaaata aaaaaacagg
     1861 ggtaaaatca aaatgacagt attaaaagga gataacttag aaatattaaa aactattgaa
     1921 tcctcaagta ttgatttaat ctatatggac cctcctttct ttacacagaa aacccaaaaa
     1981 ttatctaata acaaaaatat tatgtattca ttcgaagata cgtggacttc gattgaggat
     2041 tacaaagaat ttttgtctgt aagattagaa gaatgcaaaa gagtgctaaa aaatagtggc
     2101 agtattttcg ttcattgtga taaaattgca aatcatcata ttagattaat tttagataat
     2161 atctttggag cagatatgtt tcaaagcgaa attatatgga actataaacg gtggtctaat
     2221 tcaaaaaagg gattattgaa caatcatcaa aacatttact tttattcaaa gtcaaaagat
     2281 tttaaattta atacaatttt tacagagtat tcttctacta caaatatcga ccaaatacta
     2341 gtagaacgaa aacgaaatgg aaactctaaa actatatata aagttgataa taatggtaac
     2401 tatattctag caaaagagaa aaatggagtt cccctttcag atgtttggaa tataccattt
     2461 cttaatccaa aagctaaaga aagagtaggt tatcctacac aaaaacctat tctgttatta
     2521 gaacaaatta taaagattgc tactgataaa aatgatatag ttttagaccc gttctgtgga
     2581 agtggaacta ctttagtagc ctccaagatt ttgaatagaa attatatggg gattgattta
     2641 tctgaggaag ctatcaatat aactcagcaa cgtctggaaa atgttataaa aacaagttca
     2701 aatttattga ataaaggaat cgaagcatat agaaccaaaa ctgaggaaga ggaaaacatt
     2761 cttaaattat tacaggcaaa aattgttcaa agaaataaag gaattgatgg ttttttacct
     2821 aaacattttc aaaaaaaacc gatacctata aaaattcaaa aaaataatga atgtctgaat
     2881 gagagtatct ctttattaca gaatgctata aactccaaaa aacttgattt tggagtagtt
     2941 ataaaaactc attcagataa tttattattt gattttgata cgattcctga aaatataata
     3001 gtagttgacc attttgaact tacaatcgaa aaatggttaa gtaaaagtca acagttacta
     3061 tagtttgcaa attatttaca gaaagaacaa aaaatgctct gaaacactga tttcaaggca
     3121 ttttttgtta gtttaaaatt actcccattc ttcttttcca aacgtacaat atatccaaaa
     3181 ccattcaaaa tactagattc tattttttat aatatcacta aatccaccta attataggac
     3241 gttttcagat ttttagtccc gtcccagtcc cagtaccgga gaaatattgt tttaatataa
     3301 tatctctttt tgtcttctaa gctcttaaaa gcaaaagaac aagtaaagag tcaagacaag
     3361 gataaaaagt ccatattagg gcaaataaaa agctttaaga cagatgacaa atctaagtca
     3421 aataagaaag accatagcaa aggtgcagag agataaatat tggcggtctt cggactgcct
     3481 ttattttttt atccattttt caaatcaaat ttattcagac tatatatgca catatacact
     3541 taaattcata taaaaacatg gcttgtaaaa aattacttta atcacaataa tcgcatttaa
     3601 aattgtgatg tttgcaagct aaattacgga cttcacttgg aagttttccc ttgtatcttt
     3661 tataatagat agaaaatttg ctggcagatg aatatccaac agattctgct atctctttta
     3721 taggtagttc agtgtttaaa agaagagttt cagctacatt cattcttttt ctttgagtgt
     3781 actctgtaat gctttgacaa tatttttcct taaataaatt ttttaattta gtaccactca
     3841 ttttagatat tttttcaagc gtgccttgat ttacattcgt tgcaaaatga tcatctaaga
     3901 atcttgctac atcttcaagt gctttatcat catcaatttc aattttatat ttttttctat
     3961 ttaagtatgt gtcaattact atacttatcc attcatttgc ctttgcttta aagaaaaaat
     4021 cagcggcagg agcgtccatc ttacaattta atatttccat tgccactctt tctaaggcct
     4081 ttgtaagtat tatttgattc ggttgaagca aggttgaata aaaagattct ggattaatgt
     4141 taatagatgc taaatgtttt tctattagct cttttttaaa accaatggaa acagcaagat
     4201 aacaacaatt ctcgtgtaat aaaaaaacaa aatcatcttt tatattatca aaatcaaaag
     4261 tacataaaga gtttgcggta atagtttgat acggattaaa cttttctccg tttgcactga
     4321 caatgtaact tgaataaatt gaaacatagt ctgacatact ataagtgcta ttttgaacta
     4381 cttcctcttt gatataaaaa tcatgtatat cgataatgaa gctgcctcct tcataaaacc
     4441 aatataggcc ttctgcataa gttgaattct cttttttcca acaaaatgta tgacctgcac
     4501 ttgaatactt ctcattgttt ttacattcat ctactttcat ataatctttt acaaaatcat
     4561 aatatgacat aacacactat cccttttaga caatattcca attagcctta ttaattcaaa
     4621 actattgtat tagtaattat aacagatgta taatagaaaa gcaatgatag atattatcaa
     4681 ttaagcgaat ttatatctaa aagggatatt aaagaaagga gatatgctta tgaagattta
     4741 caaaaaacta tttgcttatg tccaagataa gaaatatctt ggggttttgg ccataatttt
     4801 ttctgctata tctgctgtac ttacagtata tggatattat ttaatctaca aatttctaga
     4861 taagttaata attaattcaa acttatccgg tgcagagagt atagcattaa aatctgttat
     4921 tacactaaca agtggagcga tattttattt tgtctcagga atgttttcac atatcttggg
     4981 atttaggctt gaaacaaatt taagaaaaag gggaatcgat ggtctggaaa aagcaagttt
     5041 taggttcttt gacttaaatc catctggtca aataagaaag atcatagatg ataatgcagc
     5101 acaaactcat caggtggtag ctcacatgat tccagatagc tctcaggcaa tagtcacacc
     5161 tatacttgtc cttgtacttg gctttatagt aagtataaga gttggcataa ttttgcttgc
     5221 tcttactata attggtggct taattttagg ggcaatgatg ggcgagcaag aatttatgaa
     5281 gatataccaa gaatccctat ctaaactaag tgctgaaact gttgagtacg tgagaggaat
     5341 gcaagttgta aaaatattta aagcaaatgt agagtctttt aaaagctttt ataaggcgat
     5401 aaaagattac tcaaagtatg cttatgatta ttccctatct tgtaaaaggc cttatgtttt
     5461 gtatcaatgg ttattttttg gactgattgc aattttaatt attcctatag tttattttat
     5521 gactagctta gctagcgcag aggtgatttt acttgagctt atcatgattt tatttttatc
     5581 aggagttctc tttgtttcat tcatgagaat gatgtggtac tccatgtata tttctcaagg
     5641 aaattatgca gtagatactt tagaggcgct ttacgaagat atgcaaaaag acaaattagt
     5701 gcatggtaat gtcaataatt ttaaaaacta taatatagaa tttgagaatg ttagctttgc
     5761 ttataatgat aaagctatca ttgaaaattt atcctttaat ttagaagaag gaaagtccta
     5821 cgcacttgtc ggttcatctg gatcaggcaa atcaacagta gcaaaactta tatcaggttt
     5881 ttacaatgtt aataaaggaa gcataaagat aggcgggata gcaataagtg aatattctga
     5941 cgaagcctta attaaagcca tttcctttgt ttttcaagat tcaaaattat tcaagaagag
     6001 catttatgat aatgtagctt tagctaataa agatgcgacg aaagatgacg ttatgagagc
     6061 cttaaaatta gcaggatgcg atttaatatt agacaaattc ccagaaagag aaaatacaat
     6121 cataggctca aaaggtgttt atttatccgg tggagaaaaa caaagaattg caattgctag
     6181 agcaatttta aaggattcca aaattattat tatggatgaa gcatcagcat ctattgaccc
     6241 agataacgag tttgaattgc aaaaagcttt taaaaatctt atgaaggata aaacagttat
     6301 catgattgca cacaggctat ctacaattaa agaccttgat gaaattattg tcatggatag
     6361 tggaaaaatt atagaaagag ggtctgacaa agaattaatg tcaaaagata caaggtataa
     6421 gagcctgcaa gagatgttta acagtgcgaa tgaatggagg gtttcaaatg aaagagtttt
     6481 ataaaaaaag atttgctctt acagatggag gagcaagaaa tttaagtaaa gcaacactgg
     6541 cttcattttt cgtttattgt ataaacatgc ttcctgccat attacttatg atttttgctc
     6601 aggaagtttt ggaaaatatg ggcaaaagca atggctttta tatagtattc tcagttttga
     6661 ctttgatagc aatgtatatt ttgctttcta tcgaatacga taaattatat aacacaacct
     6721 atcaagaaag tgcagattta agaataagga cagcggagaa tttatcaaaa ttacctctat
     6781 cttacttttc taaacatgac atttccgaca tttcacaaac aatcatggct gatattgaag
     6841 gcatagagca tgcaatgagc cactcaatac caaaggtggg cggcatggta ctgtttttcc
     6901 cattaatatc tgtaatgatg ctagcgggca atgtcaagat gggtttagct gtaattattc
     6961 catctatttt aagctttata tttatacctt tatctaaaaa atatcaggtt aatggacaga
     7021 atagatatta tgatgtctta agaaaaaact cagaaagctt tcaagaaaat atcgaaatgc
     7081 aaatggagat taaagcatat aatttatcga aggatattaa agatgactta tataaaaaaa
     7141 tggaagatag tgagagaata cacttaaagg cggaagtaac tacaatttta actttgtcta
     7201 tatcttcaat atttagcttt atatctcttg ctgttgtgat atttgtcggc gtaaatctaa
     7261 ttattaataa agagataaat tctctctacc ttataggata tttactagct gctatgaaga
     7321 taaaagactc tttagatgca tctaaagagg gcttgatgga aatattttat ttatcgccca
     7381 aaatagaaag attaaaagaa attcaaaatc aagatttaca agaaggcgat gactatagct
     7441 taaaaaaatt tgatattgat ctaaaagatg ttgagtttgc ctacaataaa gacgcaaaag
     7501 ttttaaatgg tgtaagtttt aaagctaagc agggagaggt cactgctttg gtaggtgcaa
     7561 gtggctgcgg taaaacaact atcttgaaac ttatatcaag actttatgat tatgacaagg
     7621 gacaaatctt aatcgatggc aaagatataa aagaaatatc aacagaatct ctttttgata
     7681 aggtctctat agttttccaa gatgtggttc tctttaatca aagcgttatg gaaaatatta
     7741 gaatcggtaa gcaagatgca agtgacgaag aggttaaaag agcagcaaaa cttgcaaatt
     7801 gcacagattt tatagaaaaa atggataaag gtttcgatac agttattggt gaaaacggag
     7861 ctgagctatc aggaggagaa agacaaagat tatcaatagc cagagccttc ttaaaagatg
     7921 cgccgatatt gatcttagat gagataacag caagccttga tgttaacaac gagaaaaaga
     7981 ttcaagagtc tttaaataat ttagttaaag ataaaactgt tgtaatcatt tcacatagaa
     8041 tgaaatccat agaaaatgca gacaagatag tagttcttca aaacggaaga gtagaaagcg
     8101 aaggtaagca tgaagagctt ttacaaaaat caaaaattta caaaaattta atagaaaaga
     8161 caaaaatggc agaagaattt atttattagg aggactacaa tggataataa aaaattaaaa
     8221 gtaaaagatt tagtaagtat cggtgttttt ggcgtaattt attttgcctt catgtttgga
     8281 gttggtatga tgggcttgat tccaatattg ttcttaatat acccgacagt attagccata
     8341 gttgcaggaa ctgttgttat gttatttatg gctaaggttc aaaagccatg ggcactattt
     8401 atatttggta tgatatcacc acttgtgatg tttgcagctg gtcataccta cgtagttgtg
     8461 gttttatcac ttatagtaat gataatagca gaattaatta gaaagattgg taattataat
     8521 tcatttaaat acaatatgct ttcttatgca atcttcagca catggatatg tagctcttta
     8581 atgcaaatgc ttttagcaaa agaaaaatat atggagtggt ctttgatgac gatgggaaaa
     8641 gattatgttg atgtattaga aaagttaata acttatcctc acatggcttt aatagcctta
     8701 ggtgctttct taggaggaat tcttggagca tatataggca aggctctatt gaaaaaacac
     8761 ttttcaaatg gattatattg tgtgggatac tttactcctt gcctaatttt atggtgttat
     8821 ctgaattaaa ccctatagtt aagatgtttt tgagtatacc tattgttatt agaatgttta
     8881 ttttaccatt tatggcagca agctttatga taaagacctc ggatgtaggc gcaataattt
     8941 catcgatgga taagcttaag atttcaaaga atgtatccat acctattgcg gttatgttta
     9001 gattcttccc atcttttaag gaggagaaga aaaacatcaa aatggctatg agagtaagag
     9061 ggataaattt taaaaaccca gtcaaatatc ttgaatatgt ttctgtgcca ctactcatta
     9121 tatcatctaa tatatcagat gacattgcaa aagcggcaga aacaaaggca atagaaaatc
     9181 caattgccaa gaccagatac attcgcgtaa agatacagct aattgatttt gtttatgttt
     9241 tagcggttgc tggacttatt gtgggaggct taatatggtt gaaataaaaa atttaagtct
     9301 tgattatggt gaagagcata tattagatga tatatcacta tccatagccg agggagagtg
     9361 cgtgctattt acaggaaaaa gtggaagtgg taagtcatct ttaataaatt caatcaatgg
     9421 actagctgta aggtatgata acgcaaagac aaagggcgaa ataattattg atggtaagaa
     9481 tataaaaaat ttggaacttt atcaaatctc aatgcttgtt tcaactgttt ttcaaaatcc
     9541 taagacatat ttttttaatg tcaatacgac attagaatta ttattttatt tggaaaatat
     9601 cggtcttgca agagaagaga tggacaggcg tttgaaggat atacttgaga tattcccgat
     9661 aaaaaatctt ttgaacagaa atatatttaa tctatccggc ggtgaaaaac aaattctttg
     9721 cattgcagct tcttatatag caggtacaaa gattatagtt atggatgagt cttcatcgaa
     9781 tttagatatt aaaagcataa gtgttttggc aaagatgcta aagatattaa aagagaaagg
     9841 cataagcata attgttgcag agcatagaat ttattatttg atggacatag ttgaccgtgt
     9901 atttttaata gataaaggaa agcttaaaaa aacttatact agaagtgaat ttttaaagct
     9961 agataaaaat gaattaaatg ctttaagttt aagagataaa gaattaagta aattaaaagt
    10021 tccttattta aaagaaggtg gagagtatca gataaaaaat cttagctaca aatttactga
    10081 tgatgagtgt ttaagcttaa aagatatttc gttcaagctt gggaaaattt atggcataat
    10141 aggatccaac ggacgaggaa aatcaacgct tttaagatgt ttaataggtc ttgagaaaaa
    10201 atcaaaagaa gaaatttatt ttaagggaga gaagctatct aaaaaagaaa gactcaaaaa
    10261 ctcttcactt gttatgcaag atgtaaatca tcaattattc acagatgaag tattcaacga
    10321 gcttagatta ggagtaaaga attttgatga agaaaaggcg aaaatcattt taaaagattt
    10381 aggcctggac gaatttattg aaaggcatcc gatgagttta tcaggagggc aaaagcaaag
    10441 gcttgcaata gcatctgtta tgtgcaagac ttctccattt atcttttttg acgaaccttc
    10501 aagtggtata gattattcca atatgataaa aatatctgaa ctgattaata agtataaaac
    10561 catggataaa ataattttta ttgtttccca tgatatagaa tttttaaatg aagtggcaga
    10621 tgaaattttt gaattgtaaa atagagtctg ttaaacatgt ctatagaact ggtaaattgt
    10681 ctaaaaattt aaaagatact aacacatatt gatatttatt agaaaattaa cttcaggatt
    10741 tttacagtat ttataaactt tataatatgt atttatagta ttaataattt ccatatcttt
    10801 agggaaatta tttatttact taccataatt aaagagatga gagataaata tcggcagtct
    10861 tcggactgcc tttatttttt tacttaattt aatgcagtaa atcatacgat tgaaaggaat
    10921 ctgcgttata tgctataatt aggtagttaa tctatacatt tagaaaggta aaatatagaa
    10981 ttggagaatg atatgggatt ttcatataat aaattgtaac c
//
LOCUS       DAVBCN010000063        10989 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVBCN010000063 DAVBCN010000000
VERSION     DAVBCN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10989
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.063"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            136..2316
                     /locus_tag="VQ497_001599"
     CDS             136..2316
                     /locus_tag="VQ497_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2517892.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDTSQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKNLTEEVYSVADGQVVGLEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            2368..3183
                     /locus_tag="VQ497_001600"
     CDS             2368..3183
                     /locus_tag="VQ497_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET2517893.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETLVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            3323..4666
                     /locus_tag="VQ497_001601"
     CDS             3323..4666
                     /locus_tag="VQ497_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2517894.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLEIVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            5043..6233
                     /locus_tag="VQ497_001602"
     CDS             5043..6233
                     /locus_tag="VQ497_001602"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET2517895.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            6786..7721
                     /locus_tag="VQ497_001603"
     CDS             6786..7721
                     /locus_tag="VQ497_001603"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HET2517896.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQYVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            7755..8792
                     /locus_tag="VQ497_001604"
     CDS             7755..8792
                     /locus_tag="VQ497_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET2517897.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNSSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            8965..9570
                     /locus_tag="VQ497_001605"
     CDS             8965..9570
                     /locus_tag="VQ497_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET2517898.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            9726..10256
                     /locus_tag="VQ497_001606"
     CDS             9726..10256
                     /locus_tag="VQ497_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HET2517899.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            10277..>10989
                     /gene="rlmN"
                     /locus_tag="VQ497_001607"
     CDS             10277..>10989
                     /gene="rlmN"
                     /locus_tag="VQ497_001607"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET2517900.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQGIVQESADGTVKYLFELPDGMLIETVLMCQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERISHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSISGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFP"
BASE COUNT         3341 a         2017 c         2394 g         3237 t
ORIGIN      
        1 tagaaatttt ttagaaaaaa gtgtaatttt tttataaaag cctttacaaa aaaaattaaa
       61 gtggtataat taaatcatag aaggtgcaaa cgttttcgta aaacgtttgc ctaaataaaa
      121 ataaaggaga tttgaatgat gaaagataca ttcaaaaatg tcttgtcttt cgaattttgg
      181 caaaaattcg gtaaggcttt gatggtagtt atcgcggtta tgccggctgc tggtttgatg
      241 atttcaatcg gtaagtctat cgtgatgatt aacccaacct ttacaccact tgtcatcaca
      301 ggtggaattc ttgagcaaat cggttggggg gttatcggta accttcacat tttgtttgcc
      361 ctagccattg gaggaagctg ggctaaagaa cgtgctggtg gtgctttcgc cgctggtctt
      421 gccttcatct tgattaaccg tatcactggt acaatttttg gtgtatcagg cgatatgttg
      481 aaaaatccag atgctatggt aactactttc tttggtggtt caatcaaagt tgctgattac
      541 tttatcagtg ttcttgaagc tccagccttg aacatggggg tattcgtagg gattatctca
      601 ggttttgtag gggcaactgc ttacaacaaa tactacaact tccgtaaact tcctgatgca
      661 ctttcattct tcaacgggaa acgtttcgta ccatttgtag ttattcttcg ttcagcaatc
      721 gctgcaattc tacttgctgc tttctggcca gtagttcaaa caggtatcaa taacttcggt
      781 atctggattg ccaactcaca agaaactgct ccaattcttg caccattctt gtatggtact
      841 ttggaacgtt tgctcttgcc atttggtctt caccacatgt tgactatccc aatgaactac
      901 acagctcttg gtggtactta tgacatttta actggtgcag ctaaaggtac tcaagtattc
      961 ggtcaagacc cactatggct tgcatgggta acagaccttg taaaccttaa aggtactgat
     1021 actagtcaat atcaacactt gttagataca gtacatccag ctcgtttcaa agttggacaa
     1081 atgatcggtt cattcggtat cttgatgggt gtgattgttg ctatctaccg taatgttgat
     1141 gctgacaaga aacataaata caaaggtatg atgattgcaa cagctcttgc aacattcttg
     1201 acaggggtta ctgaaccaat cgaatacatg ttcatgttca tcgcaacacc tatgtatctt
     1261 gtttactcac ttgttcaagg tgctgccttc gctatggctg acgtcgtaaa cctacgtatg
     1321 cactcattcg gttcaatcga gttcttgact cgtacaccta ttgcaatcag tgctggtatt
     1381 ggtatggata tcgttaactt cgtttgggta actgttctct ttgctgtaat catgtacttt
     1441 atcgcaaact tcatgattca aaaattcaac tacgcaactc cagggcgcaa cggaaactac
     1501 gaaactgctg aaggttcaga agaaaccagc agcgaagtga aagttgcagc aggctctcaa
     1561 gctgtaaaca ttatcaacct tcttggtgga cgtgtaaaca tcgttgatgt tgatgcatgt
     1621 atgactcgtc ttcgtgtaac tgttaaagat gcagataaag taggaaatgc agagcaatgg
     1681 aaagcagaag gagctatggg tcttgtgatg aaaggacaag gggttcaagc tatctacggt
     1741 ccaaaagctg acattttgaa atctgatatc caagatatcc ttgattcagg tgaaatcatt
     1801 cctgaaactc ttccaagcca aatgactgaa gcacaacaaa acactgttca cttcaaaaat
     1861 cttactgagg aagtttactc agtagcagac ggtcaagttg ttggtttgga acaagtaaag
     1921 gatccagtat ttgctcaaaa aatgatgggt gatggatttg cagtagaacc tgcaaatgga
     1981 aacattgtat ctccagtttc aggtactgtg tcaagcatct tcccaacaaa acatgctttt
     2041 ggtattgtga cggaagcagg tcttgaagta ttggttcaca ttggtttgga cacagtaagt
     2101 cttgaaggta aaccatttac agttcatgtt gctgaaggac aaaaagttgc agcaggagat
     2161 ctccttgtca cagctgactt ggatgctatc cgtgcagcag gacgtgaaac ttcaacagta
     2221 gttgtcttca caaatggtga tgcaattaaa tcagttaaat tagaaaaaac aggttctctt
     2281 gcagctaaaa cagcagttgc taaagtagaa ttgtaatata cttgaggttg gaagctgtat
     2341 tccaacctct tattttggga gaaaagaatg aaatttttaa cactcaatac tcacagttgg
     2401 atggagaaag aagcagagga aaaattccag attttgcttg aagatattct tgaaaaggac
     2461 tatgatttga tttgtttcca agaaatcaat caggagatga cctcgtcaga ggtggaggtt
     2521 aatgaccttt atcaagcttt gccagcagct gagcctattc accaagacca ttatgttaga
     2581 ctcttggttg aaaagttgtt agagcaaggg aaaaattact actggacctg ggcttataac
     2641 catatcggct atgaccgcta ccacgaaggt gtggctatct tgtctaaaac acctattgaa
     2701 gccagagaaa ttttggtttc agatgtggat gatccaacag actatcatac tcgccgtgtt
     2761 gccctagctg aaactttagt cgatggcaag gagctagcag ttgccagtgt tcatctctct
     2821 tggtgggata aaggtttcca agaagaatgg gcacgatttg aggctgtctt gaaaaaattg
     2881 aacaagccac ttttactagc tggagatttt aacaacccag caggtcagga aggttaccaa
     2941 gctattttag ctagtccatt aggcttacaa gacgcatttg aagttgctca agagaaaagt
     3001 ggtagctata ctgttccgcc tgaaattgat ggctggaaag ggaacactga accccttcga
     3061 atcgattatg tctttactac caaagagtta gcggtggaaa atttacatgt cgtatttgat
     3121 ggtaacaaga gtccacaagt gagtgatcac tatggcttga atgctatgtt aaactggaaa
     3181 taataactga aaagaggttg gaactataaa attccagcct tttcttacta gagaagctac
     3241 tggaaatagc ctaaataagt gagactactg taatggaata aaatatggta taattgataa
     3301 gatagataga atcgaggatg ttatgtcatt tacgaaattt caatttaaaa actatattag
     3361 agaagccttg aaggagttaa aatttacaac tccaacagag gtgcaagata agttgattcc
     3421 tattgttttg gcaggtcgtg acctagtagg agaatcaaaa acaggttcag gtaagactca
     3481 tactttcttg ttaccgattt tccagcaatt agatgaagct agcgatagtg tacaagcagt
     3541 gattactgca ccgagtcgtg agttggctac tcaaatttac caagtagcgc gtcagatttc
     3601 agctcactca gatgtcgaag ttcgtgtggt taattatgtg ggtggtacgg ataaggctcg
     3661 ccagattgag aaattggcaa gcaatcagcc tcatattgtt attggaacac caggccgtat
     3721 ctacgacttg gttaaatctg gtgatttagc tattcataaa gccaagacat ttgttgttga
     3781 tgaagcagat atgaccttgg atatgggatt cttggaaatt gttgataaga ttgctggcag
     3841 tcttccaaaa gacttgcaat tcatggtctt ctcagcgact atcccacaaa aactgcaacc
     3901 attcttgaaa aaatacttat caaatcctgt tatggagaaa attaagacta aaacggttat
     3961 ttctgacacc attgataatt ggttgatttc gaccaaggga catgataaga atgctcaaat
     4021 ttaccaattg actcagttga tgcagccgta tttggcaatg atttttgtta acactaaaac
     4081 gcgtgctgat gaattgcatt catatctgac tgctcaaggc ttgaaggttg caaaaatcca
     4141 tggagatatt gcccctcgtg aacgcaagcg aatcatgaat caggtgcaaa atctggattt
     4201 tgagtatatt gtcgcaacag atttagcagc gcgtgggatt gacattgaag gtgtcagcca
     4261 tgtcatcaat gatgccattc cgcaagactt atcttttttt gttcaccgtg ttggtcgtac
     4321 tggacgaaat ggcctaccag gtacagctat taccctttat cagccaagtg atgactcgga
     4381 tatccgtgag ttggagaaat tgggaatcaa gtttagtcct aagatggtca aagatgggga
     4441 atttcaagat acctatgacc gtgatcgtcg tgccaaccgt gagaaaaaac aagataaact
     4501 tgatatcgaa atgattggtt tggttaaaaa gaaaaagaaa aaagtcaaac cgggttataa
     4561 gaagaaaatt caatgggcgg ttgatgaaaa gcgccgtaaa actaagcgtg ctgaaaatcg
     4621 cgctcgcggt cgtgcagagc gtaaagctaa acgccaaaca ttttaataga aattgttgga
     4681 gtactgagct ccaacttttt tatttatgag aacgaactat ctaaaccgaa acactacatt
     4741 aaagactgca aattgcgatt aaaaatggta taatgataaa gttatatagt cccgataaga
     4801 tggtaggtat ttattacgaa gagttttcct atcagtagtt tgcaattcgc aaagaatgac
     4861 ggcttcgagg tcgctcgtct ttctacggtt gccgccatac tgcggtcacc ctatgcgcct
     4921 tactagcttc agaaataaaa aagtatcgtt tttatttctg aagctagtaa ctcttagata
     4981 atggaaattc ctatcagtac tttgtaactc tataacaata ttttttaagg ggggacattt
     5041 ttatgtcaga acgtaaatta ttcacgtctg aatctgtatc tgaggggcat ccggataaga
     5101 ttgcagacca aatttcagat gcgattttgg atgctatttt agcaaaggat ccagaggcgc
     5161 acgttgctgc tgaaacagct gtatatactg gttctgtcca cgtttttggt gaaatttcta
     5221 caaatgccta tgtggatatt aaccgtgtgg ttcgtgatac cattgcagag attggttata
     5281 ccaatacaga atatggattt tctgctgaga cggtgggagt acacccatct ttggtggaac
     5341 aatctcctga catcgctcaa ggtgttaacg aagccttgga ggttcgtgga aatgctgatc
     5401 aagatccact ggacttgatt ggagcaggtg accaagggct catgtttgga tttgcagtag
     5461 atgaaacaga agagctcatg ccattgccaa ttgcactcag tcataaattg gttcgtcgtc
     5521 tggcagaact tcgtaagtct ggagaaatta gctatctccg tccagatgca aaatcacaag
     5581 ttacagttga gtacgatgaa aatgaccgtc cggtacgtgt agatacagtc gttatttcta
     5641 ctcagcatga tccagaggcc actaatgaac aaatccatca agatgtgatt gacaaggtca
     5701 tcaaagaagt tattccatct tcttatcttg atgataagac aaaattcttt atcaatccga
     5761 caggtcgttt tgtaatcggt ggtcctcaag gggactcagg tttgactggt cgtaagatta
     5821 ttgtagatac ttatggtggc tactctcgtc atggtggtgg tgccttctct ggtaaagatg
     5881 cgactaaggt ggatcgttca gcctcttatg cggctcgcta tattgccaag aatatcgttg
     5941 cagcaggcct tgctaagaag gcagaagtgc agttggccta tgctatcggt gttgcgcaac
     6001 ctgtttctgt tcgtatcgat actttcggta caggaacagt agctgaaagt caacttgaaa
     6061 aagcggctcg tcaaatcttt gaccttcgcc ctgcagggat tatccaaatg ctggacctca
     6121 agcgtccaat ttaccgtcaa acatcggctt acggtcacat gggacgtaca gatattgatc
     6181 ttccatggga acgtttggat aaggtagatg ctttgaaaga agcagtaaaa taagatttta
     6241 agaggggaac gtcctctctt ttttatagtt tttaactata ctgggatact gttctgaaaa
     6301 tccattttgc gaaagtagag atttacatgt ataatggatt gaaactagaa tagtacacct
     6361 caacttctaa aacattgtta gaaatcgatt tgactgtcct gatcgatttc tcctgttctt
     6421 gtttcatttt actatatttc tttaaaaatg ataaaggttg agatttctcc tcgtaataga
     6481 taatcatggg gatatttcaa tccaaagttt tattcgttat cacttgacta ttgcaaggtt
     6541 ttctagagca acagagtcat ggaatggact catggttgag atttctcctt gttgcttgga
     6601 cttcattcaa aagtctgtta cccaagcctt gttcaaactt ccaatacact agctgtttcc
     6661 atagcatgac ttctgtacta gactttcttt tccgaataaa tagatagaac cacagaatct
     6721 agtaaaccta gaattaaaat tatggtataa tattagcaat aaaagaaatc tggaggatta
     6781 gaatcatggt atcaacgaaa acacaaattg ctggttttga gtttgacaat tgcttgatga
     6841 atgcagcagg tgtggcttgt atgacgatag aggagttaga agaggtcaaa aactcagcgg
     6901 caggaacctt tgttactaag acagcgacct tggacttccg tcaggggaat cctgagccac
     6961 gctaccaata tgttccactt ggttccatca actctatggg cttgccaaat aatggcttag
     7021 actattattt ggattatctt ttagatttgc aggaaaaaga gtcgaaccga actttcttct
     7081 tatctctggt cggcatgtct ccagaggaaa cccatactat tttgaaaaaa gtccaagaga
     7141 gtgattttcg tggtctgact gagctaaatc tttcctgtcc aaatgttcca ggtaaacctc
     7201 agattgccta tgattttgag acaacagacc ggattttggc agaagtgttt gcttacttca
     7261 ccaaacctct tggaattaaa ttgccacctt attttgatat tgttcacttt gaccaagcgg
     7321 cagctatttt caacaaatat ccgctcaagt ttgtcaactg tgttaactct atcggaaacg
     7381 gcctctatat agaagacgaa tctgtcgtta ttcggcctaa gaatggtttt ggtggaattg
     7441 gtggagaata catcaaaccg actgctctag ccaatgttca cgccttttat caacgtttaa
     7501 atcctcaaat ccaaattatc ggaacaggtg gcgttctgac tggtcgagat gcctttgaac
     7561 acatcctctg tggagcaagt atggtgcagg tgggaacgac ccttcacaaa gaaggcgtca
     7621 gtgcttttga ccgcattacc aatgaactga aagcaatcat ggtggaaaaa ggctacgaga
     7681 gcttagaaga tttccgtggg aaattgcgct atattgacta aattaaatcg aaaaatctga
     7741 agaaaggaga gacgatgcta gccattgaag aaagtcagaa gttgacttta tcaaatttac
     7801 cgagcctgag cctatttaca gggacagatc agggtcagtt tgaagtgatg aagagtcaag
     7861 tgttgaaaca gattgggtat gattctgctg acctcaactt tgcctacttt gatatgaaag
     7921 aagtagttta caaggatgtg gaactggagt tggtcagcct tcctttcttt gcggatgaga
     7981 aaatcgtgat attagattat tttatggata tcacgactgc taagaaacgc tttttgacag
     8041 atgatgagct taagtcattt gaggaatacc ttgacaattc ttctccaaca accaagttga
     8101 taatctttgc agaaggaaag ctggatagca aaagacggtt agtcaaatta cttaagcgtg
     8161 atgccaaggt cttcgatgca gtagaagtaa aagaacaaga attgcgccag tatttccaaa
     8221 agtggagtca gaaacaaggt ctgcagttta ccaatcattc ttttgaaaat ctcctcatca
     8281 agtcggggtt tcaatttagc gaaatccaga aaaatcttct ctttttacag tcctataagg
     8341 cgaattctgt tattgaggaa gaggatattg ttaacgcaat tcccaagacc ttgcaggaca
     8401 atatttttga tttaactcag tttattctga ctaaaaagat ggatcaggcg cgcgatttgg
     8461 tgagagactt gaccttgcaa ggggaagatg aaatcaaact gattgcagtc atgctgggac
     8521 aatttcggac ttttactcag gtgaagattt tggcggagtc tggccaaaca gaatcgcaga
     8581 ttgcaagtag tttaggtagt tatctgggac gtaacccaaa tccttatcaa atcaagtttg
     8641 cattaagaga ttcgagagga ctttctttga gctttctgaa gcaagctatt tcctatttga
     8701 ttgagacaga ctatcagatt aagacaggtc tttatgaaaa aggtttcctt tttgaaaagg
     8761 cactcttaca gattgctagt caggtcaatt gacatttgtt gaaactacaa cccgcgtcag
     8821 gattatcttg tcgtgggttt cttgctgatt tgcttatttt attagaaagt atgtcagtga
     8881 tttccttatc tataagcaaa aaacttgaaa aaagtgaatg tttgcgttat cattttcata
     8941 tagaaagaaa aagaggtatt acagatggct attatcttac cagaacttcc atatgcatac
     9001 gacgctttgg aaccatacat cgatgcggaa acaatgcatt tgcaccatga caaacaccat
     9061 caaacttatg tcaacaatgc caatgcagct ttagaaaaac accctgaaat cggtgaagac
     9121 cttgaagcct tgcttgctga tgtagaatct atcccagctg atatccgtca agcacttatc
     9181 aacaatggtg gcggacactt gaaccacgct cttttctggg aattgatgac tcccgagaaa
     9241 acagctcctt cagcagaact ggcagcagca atcgatgcaa catttggttc atttgaagaa
     9301 ttccaagcag ccttcactgc agcagcaaca actcgttttg gttcaggttg ggcatggttg
     9361 gttgtcaaca aagaagggaa acttgaagtg acttcaacag caaaccaaga cacaccaatc
     9421 tcagaaggta aaaaaccaat cttgggcttg gacgtttggg aacatgctta ctacgtgaaa
     9481 taccgcaacg tgcgtcctga ctacatcaaa gctttctttt cagtaatcaa ctggaataaa
     9541 gtagatgaat tgtacgcagc tgctaaataa tgatagttgg agggaagaat tgttcttctc
     9601 tttttaggtt ataagattct ggtctgacaa aatcgtcaga cttttttcat ttttatgaga
     9661 aacgtgctaa ctgctagaaa ttatggtaga ataaagtatt aagtaatcgt ggaaaaagga
     9721 tatctatgcg aaaagaaatt gcacctgaat tatacaacta taacaagttt cctggtccgg
     9781 agttccattt acacggggat aaggtcgaaa cggaagggat agctttttcc ttggttgaaa
     9841 atatcaagga tgcctttgat gtgacgactt ttaatcagcg tttttcagaa gtcttaacca
     9901 agtttgatta tatcgtgggg gactggagca acgaacagct tcgcctacga ggtttttaca
     9961 aggatgaccg aacagaagaa aaacttgaaa aaatcagtcg tttacaagac taccttttag
    10021 agtattgtag ttatggttgt gcctattttg tcttagaaaa tgaagcccct aagcgagcat
    10081 catttgacaa gaaaatgcgt aagacggaag aagaaacacc ttctagaaaa ggaaagaaac
    10141 cgactcaaac taaacgaaag tcgaatgcag ataagaaaaa tagacgtcgt cagaaagacc
    10201 agcattctca gaaagaggac aaggaacaac gtcattttgt cattcgtcag aagtgagtag
    10261 agaataagga gaagaaatga aaccctcaat tcatagtttg gcacatcaaa ccatgcagga
    10321 atgggtattg gagcagggag aaaagaaatt ccgtgcagat caaatctggg aatggctcta
    10381 ccgtaaacgt gtgcagtcat ttgaagaaat gaccaacctt tccaaggatt tgattgctaa
    10441 gctcaatgac cagtttgtgg tcaatccctt gaaacaaggt attgtgcaag agtctgctga
    10501 tggtacagtc aaatatctct ttgaattgcc tgacggtatg ttaattgaga ctgtactcat
    10561 gtgtcaacac tatggtttat ctgtctgtgt gaccactcag gtcggctgta atatcggttg
    10621 taccttctgt gcctctggtt tgattaagaa gcagcgtgac ctcaataacg gtgaaatcgt
    10681 agcgcaaatc atgctggttc agaaatactt tgatgagcgt ggtcaggatg aacgcatcag
    10741 ccatatcgtt gtcatgggaa tcggtgagcc ctttgataat tacaacaatg tcttgaattt
    10801 ctttcgtact atcaatgatg ataaaggaat ggctatcggt gctcgtcaca tcacggtttc
    10861 aatctcaggt ttggctcata aaattcgtga ctttgctgat gaaggggttc aggtcaatct
    10921 tgccgtgtct cttcacgcac ccaacaatga acttcgttca agtatcatga agattaaccg
    10981 tgcctttcc
//
LOCUS       DAVBCN010000064        10343 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVBCN010000064 DAVBCN010000000
VERSION     DAVBCN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10343
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.064"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..266
                     /locus_tag="VQ497_001608"
     CDS             <1..266
                     /locus_tag="VQ497_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HET2517901.1"
                     /translation="SIDERLRTRLRVIIWKQWKKKSRRLWGLLKLGVPKWIADKVSGW
                     GDHYQLVAQKSVLKRAISKPVLEKRGLVSCLDYYLERHALKVS"
     gene            720..1898
                     /locus_tag="VQ497_001609"
     CDS             720..1898
                     /locus_tag="VQ497_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2517902.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            2213..2344
                     /locus_tag="VQ497_001610"
     CDS             2213..2344
                     /locus_tag="VQ497_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HET2517903.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            2365..3915
                     /gene="dltA"
                     /locus_tag="VQ497_001611"
     CDS             2365..3915
                     /gene="dltA"
                     /locus_tag="VQ497_001611"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HET2517904.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEALVQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMIMDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGVTHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQSLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            3912..5156
                     /gene="dltB"
                     /locus_tag="VQ497_001612"
     CDS             3912..5156
                     /gene="dltB"
                     /locus_tag="VQ497_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HET2517905.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQTIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            5170..5409
                     /gene="dltC"
                     /locus_tag="VQ497_001613"
     CDS             5170..5409
                     /gene="dltC"
                     /locus_tag="VQ497_001613"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HET2517906.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDICVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            5402..6670
                     /gene="dltD"
                     /locus_tag="VQ497_001614"
     CDS             5402..6670
                     /gene="dltD"
                     /locus_tag="VQ497_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HET2517907.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKGELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            6825..7265
                     /gene="adcR"
                     /locus_tag="VQ497_001615"
     CDS             6825..7265
                     /gene="adcR"
                     /locus_tag="VQ497_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HET2517908.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            7265..7969
                     /locus_tag="VQ497_001616"
     CDS             7265..7969
                     /locus_tag="VQ497_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET2517909.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            7962..8768
                     /locus_tag="VQ497_001617"
     CDS             7962..8768
                     /locus_tag="VQ497_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET2517910.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            8778..10283
                     /gene="adcA"
                     /locus_tag="VQ497_001618"
     CDS             8778..10283
                     /gene="adcA"
                     /locus_tag="VQ497_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HET2517911.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDVEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISIMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
BASE COUNT         2955 a         1935 c         2236 g         3217 t
ORIGIN      
        1 ccagcataga tgagcgcttg cgtactcgcc tacgagtgat tatctggaag caatggaaga
       61 agaaatcgag acgattatgg ggattgctta agttaggagt tcctaaatgg atagcagata
      121 aggtatctgg ctggggcgac cattatcaat tagtagctca gaagtcggta cttaaacgtg
      181 ctatatcaaa accagtcctg gaaaaacgtg gactggtttc gtgtttggat tattaccttg
      241 aacgacatgc gttaaaagtt agttgaaccg ccgtatgccg aacggcacgt acggtggtgt
      301 gagaggggct agagattatc ctctactcga ttaactcccc tgaaatttgt tttaattatg
      361 caaatttcac gtatttttga tgctgagacg acgatcctgg gaacttttca gatatttttt
      421 tgactatcta aaatctatca ttagaaaagc ttagagcgcc aaaggatttg agcgtttttc
      481 tgatttttaa gactttttcc cagtctcttt ttcgattgaa gatgtaatta ttctactaac
      541 taacttctta gtactagcca acaacgataa tcataattcc tcctaaaatt aggaataata
      601 aaggcaatag tttttgtttt ttcatgtaaa aaacctcact tttgttttct gctattttat
      661 gctaaaatat taaaaatcaa atttaattcc aaagtttgta actaaagggg gagcgctaca
      721 tgtctaattc atttgtcaag ttgttagtct ctcaattatt tgcaaattta gcagatattt
      781 tctttagagt aacaatcatt gctaacatat acattatttc aaaatcagta attgccacat
      841 cactagttcc tatcttaata ggaatatcct cttttgttgc gagtctttta gttccgttgg
      901 ttactaaaag gttagcgcta aatagggttt tatctttatc tcaatttgga aagactatat
      961 tattggcgat actggtagga atgtttaccg taatgcaatc cgtagcgcct ttggtgacct
     1021 atctatttgt tgttgcaatt tccatactag atggttttgc agcacccgtt tcctatgcta
     1081 ttgtgccacg ctatgcgacc gatttgggta aggctaattc agccttatca atgactggtg
     1141 aagctgttca attgataggt tggggattag gtggactctt gtttgcaaca attggtctgt
     1201 tacctaccac gtttatcatt ttagtcttgt atatcatttc tagctttctg atgttatttc
     1261 ttcctaacgc tgaagtggag gtgttagagt cagaaactaa tcttgaaatt ttgctcaaag
     1321 gttggaagtt agttgctaga aatcctagat taagactttt tgtatcagca aatttattgg
     1381 aaattttttc aaatacgatt tgggtttctt ccattatact tgtttttgta acggagttat
     1441 taaataaaac ggaaagttac tggggatatt ctaatacagc atactctatt ggtattataa
     1501 ttagtggctt aattgctttt aggctatctg aaaagttcct tgctgctaaa tgggaaagta
     1561 ttctttttcc tcttgtagct atggcaatag tgacactgac tattctatat tttccaaacg
     1621 cacagatgtt tttattattt tcagctttag ttggtatgtt atcgcaacta aaagaagttc
     1681 ctgaaagtgt atttcttcag gaaacagtag aggaaaatca tttagttaat gtctattccg
     1741 ttcttgaagt gatttctaca ttagcatttt cagtatttgt tttgctaatg agctatatta
     1801 ctgagagttt tggtattagc atcagttttt ggctatcagc catttgtctg atgatagaag
     1861 ctattttaat ttatatcaga cgagattatt ttaaatgaga gagtggttaa aaagtcttta
     1921 aaaatcagaa aagcgcatag tatcaggtgt tgaaaaaact tgatattgtg tgttttatta
     1981 tggggaagat ttacttcatt gtctcctgaa atggagtttt gaagcaggat cactctattt
     2041 gaaaatatac atagaaaaac tgtataatcc ttaaaataag ctgaaatcta ttggaaacat
     2101 acggtaaaca attgaaaaga tactatataa agtggtacaa tggtagaaaa ataagctagt
     2161 aagaatcact cttattttag cgaaaaatta cagaaatgaa tggtttttct tgatgaaaca
     2221 gagaaaagaa ttgtacctct ttcttggtcg gacagccttg tattttctta tctttctagg
     2281 gctgctttac ttctttagct atcttggtca gggtcaagga agctttatct ataatgaatt
     2341 ttaacttgaa ggagaatccc tattgtgtca aataaaccaa tagcagatat gattgaaacc
     2401 attgagcatt ttgctcagac acagcctagc tatcctgtct ataatgtttt ggggcaggaa
     2461 cacacttatg gcgatttaaa ggctgattcg gatagtttgg ctgcagtcat tgaccaacta
     2521 ggcttgcctg agaagtctcc tgtggttgtt tttggtggcc aagaatatga aatgttggca
     2581 acctttgtag cgctgactaa gtcaggtcat gcctacattc caattgatag ccattcggcc
     2641 ttggagcgag tttcagctat tttagaagta gcagagccaa gcttgattat tgccatttca
     2701 gcctttccct tggagcaggt ttctacacca atgataaatc tagctcaggt tcaagaagcc
     2761 cttgttcaag ggaataacta tgaaatcacg catccagtca agggagatga taattactac
     2821 attatcttta cttctggtac gactggtaag cctaagggag tgcagatttc acatgataat
     2881 ctcctcagct ttacaaactg gatgattatg gataaggaat ttgcgacacc gagtcgtccg
     2941 caaatgctgg cacagccacc ttattctttt gacttgtctg tcatgtattg ggcaccgacc
     3001 ttggcacttg gtggtacgct tttcactctt ccttcagtca tcactcagga ctttaagcaa
     3061 ctctttgcgg ctatcttttc attgccaatc gctatctgga catcaacacc atcctttgca
     3121 gatatggcca tgttgtctga atacttcaac agtgagaaaa tgcctggagt cacgcatttc
     3181 tactttgatg gtgaagaatt gacggtcaaa acagctcaaa aactgcgcga gcgtttccca
     3241 aatgcccgta tcatcaatgc ttacggccca acagaagcga cagtagctct ctcagcagtt
     3301 gccgtgacag acgagatgtt agcgactctc aaacgcctac caatcggcta taccaaggct
     3361 gattctccaa cctttatcat tgacgaggaa ggaaataaac tgccaaatgg cgaacaggga
     3421 gaaatcattg tttctgggcc agctgtttca aaaggttata tgaacaatcc tgaaaaaaca
     3481 gcagaagcct tctttgagtt tgaaggtctg ccagcctatc acacaggcga tgtgggaacc
     3541 atgacagatg agggcttgct tctctacggc ggacgcatgg acttccagat taagtttaac
     3601 ggttaccgca ttgagttaga agatgtctct caaagcctca acaagtctcg ctttatcgaa
     3661 tctgctgtcg cagtaccgcg ctataacaag gaccacaagg tacaaaatct attggcttat
     3721 gtcatcttaa aagacggtgt tcgtgagcag tttgagcgag atatcgatat taccaaggcc
     3781 atcaaggaag acctgacaga catcatgatg tcctacatga tgccatctaa attcctttac
     3841 cgagacagtt tgccactaac tccaaatgga aagattgaca tcaaaggatt gattaacgag
     3901 gtgaataaga gatgatggag ttttttcaac agcttcctca tttagagcca tatggcaatc
     3961 ctcagtattt tgtttatgtg attgctgcaa ccttgcccat ctttataggc ctctttttca
     4021 agaaacgctt tgcctggtat gaagtgttgg taagtctctt ctttattgtc accatgttgg
     4081 tgggtggaaa gaccaatcaa ctagctgcct tgggtattta cctttgctgg gaaatattgc
     4141 tcctgctttt ctacaagcat tatcgaaaaa gcaaggatgg caagtgggtc ttctacttag
     4201 ttagttttct gtccctactt ccgattatct ttgtcaaggt gcaaccagct atcaatggaa
     4261 cgcagtcttt gcttgggttc ttgggaattt cttacctgac ctttcgttcg gttgggatta
     4321 tcatcgagct gagagatgga gtgattaagg attttaccct ctgggaattc ctccgtttcc
     4381 ttctcttcat gccaactttc tcgagtggtc caatcgatcg ctttaagcga tttaatgaaa
     4441 attatcagac cattcctgag cgagatgagt tgatggatat gctggatgaa tctgtccgct
     4501 atatcatgcg gggctttttg tataagttta tcctagctca tgttttagga gagaccttac
     4561 tacctcctct gaagaattta gccttgcagt caggtggctt ctttaatctc tatgccttgg
     4621 cagttatgta tacttttggt ctggagctct tctttgactt tgcaggttat tctatgtttg
     4681 ctttggccat ctcaaacttg atgggaatcc gtagccctat caactttaac aagccctttt
     4741 tatcaaggga tttaaaggag ttttggaatc gctggcatat gagtctgtcc ttctggttcc
     4801 gtgactttgt ctttatgcga atggtgatgg tgttaaccag aaagaaagtc tttaaaaatc
     4861 gtaatgtaac ctcaagcatg gcctacattg taaatatgct gattatggga ttttggcatg
     4921 gtgtgacctg gtactatatc gcctatggac tctttcatgg actaggcttg gtcatcaatg
     4981 atgcctgggt tcgcaagaaa aaaacgctca ataaggaacg gaaaaaagca gggaaggctg
     5041 ccctacctga gaatcgctgg attcagttgc ttggtatggt tgtcactttc catgttgtca
     5101 tgttgtcatt cttaatcttt tctggattct tgaataatct atggtttaaa aaataaaagg
     5161 aaataaaata tggatatcaa atcagaagtt atcgaaatta ttgatgagtt gtttatggaa
     5221 gatgtttctg acatgatgga tgaagatctt tttgatgcag gtgtcttgga tagtatggga
     5281 acggttgagt tgattgtgga gattgagaac cgttttgaca tttgtgtccc tgtaacagag
     5341 tttggtcgtg acgactggaa tacagctaat aaaattatag ctggtattgt ggagctacaa
     5401 aatgcttaaa cgcttatgga tgatcttcgg accggtcttg atcgctggtt tgttggtttt
     5461 tctgctcatt ttcttttatc ctactgagat gcatcataat ctaggagctg aaaagcgttc
     5521 agcagtggct actactatcg atagttttaa ggagcgaagt caaaaagtca gagcactatc
     5581 tgatccaaat gtgcgttttg ttcccttctt tggctctagt gaatggcttc gttttgacgg
     5641 tgctcatcct gcggtattag ctgagaaata caatcgttcc taccgtcctt atcttttagg
     5701 acagggggga gctgcatcgc ttaaccaata ttttggaatg caacagatgt taccacagct
     5761 ggagaataaa caagttgtgt atgttatctc acctcagtgg ttcagtaaaa atggctatga
     5821 tccagcagcc ttccagcagt attttaatgg agaccagttg actagttttc tgaaacatca
     5881 atctggggat caggctagtc aatatgcagc gactcgctta ctgcaacagt tcccaaacgt
     5941 agctatgaag gacctggttc agaagttggc aagtaaagga gaattgtcga cagcagacaa
     6001 tgaaatgatt gaattattgg ctcgttttaa tgaacgccaa gcctcctttt ttggtcagtt
     6061 ttcggttaga ggctatgtta actacgataa gcatgtagct aagtatttaa aaatcttgcc
     6121 agaccagttt tcttatcagg caatagaaga tgttgtcaaa gcagatgctg aaaaaaatac
     6181 ttccaataat gagatgggaa tggaaaatta tttctataat gagcagatca agaaggattt
     6241 gaagaaatta aaggattctc agaaaagctt tacctatctc aagtcgccag agtataatga
     6301 cttgcagttg gttttaacac agttttctaa atctaaggta aacccgattt ttatcattcc
     6361 acctgttaat aaaaaatgga tggactatgc tggtctacga gaggatatgt accaacaaac
     6421 ggtgcagaag attcgctacc agttagaaag tcaaggtttt accaatatag cagatttttc
     6481 taaggacggc ggggagcctt tctttatgaa ggacaccatt caccttggtt ggttgggttg
     6541 gttggctttt gacaaggcag ttgatccttt cctatccaat cccacaccag ctccgactta
     6601 ccatctgaat gagcgctttt tcagcaaaga ttgggcgact tatgatggag atgtcaaaga
     6661 atttcaatag ataatataat atagaagagt tcaagaatga aactagtttt cacgtttttt
     6721 cttgactctt tttttgcttg tgatataatt aactggtaaa caaaatgtca aagaatagta
     6781 tttttctctt aaaaaagaga agtctaaaag gaaaggagtc agatatgaga cagctagcaa
     6841 aggatatcaa tgcttttttg aatgaggtga ttttgcaggc ggaaaatcag catgaaatcc
     6901 taataggtca ttgcactagc gaggtggccc tgaccaatac tcaggagcat atccttatgc
     6961 tcttgtcaga ggaatcttta acaaattcag aattggcccg tcgtctcaat gtcagtcagg
     7021 cggcagttac caaggccatt aagtctttgg tcaaggaagg gatgttggaa acatctaaag
     7081 attctaaaga tgcgcgtgtg attttttatc agttgactga cttggctcgt ccaatcgctg
     7141 aggagcacca ccatcaccat gagcatacac ttttaaccta tgaacaagtg gcgactcagt
     7201 ttactccaaa tgaacaaaaa gtgattcagc ggtttttgac tgctttagta ggagaaatca
     7261 aataatgaga tatattacgg tagaggattt gtccttctat tatgataagg agcctgttct
     7321 tgaacatatc aattatagtg ttgatagtgg ggaatttgtt accttgactg gggaaaatgg
     7381 agcggctaag acgacgctca tcaaggctag tcttggaatt ctgcaaccac gcattggaaa
     7441 ggtggctatt tcaaagacaa atacgcaagg taagaaattg agaatagcct atcttcctca
     7501 acaaattgcc agttttaatg ctggttttcc aagtacggtc tatgaatttg tcaagtcggg
     7561 tcgctatccg agaaaaggct ggttccgtcg tttgaatgct catgatgagg agcatatcaa
     7621 ggccagtctg gactcagttg gcatgtggga acatcgagac aaacgcttgg ggtctctatc
     7681 tgggggacaa aagcagcgag cggtgattgc gcgtatgttt gcttctgacc ctgatgtgtt
     7741 tatcctagac gagccgacaa cggggatgga tgcaggaagt aaaaatgaat tttacgaact
     7801 catgcaccac agcgcccatc atcatggcaa ggctgttttg atgattaccc atgaccctga
     7861 agaagttaag gattatgcgg atcgcaatat tcatctagtc cgtaaccaag actcgccatg
     7921 gcgttgtttc aacgttcatg agaatggcca ggaggtgggc catgcttagt ttattatctt
     7981 acgactttat acaacgcgcc tttttggcgg ttattgctat gagtcttttc tcaccggtat
     8041 tgggaacctt cctcatcttg cgtcgtcaga gtttgatgag tgataccctt agccacgtct
     8101 cactttcagg tgtagccttt ggtctggttt tggggatttc tccaactgtt tctactattg
     8161 ccattgtctt gattgcggcg gtctttctgg agtatctccg tacggtttac aagagcttta
     8221 tggaaatcgg gacagctatc ctcatgtcaa caggtctggc tgtttctctg attgtcatga
     8281 gcaagggtaa aagctcgagt tcaatgagtt tggaccaata tctctttggt tcgatcgtga
     8341 ctatcagtga agaacaggtc atttccctct ttgtcattgc ggcggttgtt ttgattttga
     8401 cctttctctt tcttcgtcct atgtatatct taacttttga cgaagatacg gcctttgtgg
     8461 atggcttgcc agttcgtacc atgtccattc tttttaacat ggtgacaggg gtggctattg
     8521 cccttatgat tcctgcagca ggagctcttc tggtatcgac cattatggtc ttgccagcta
     8581 gtattgccct gcgtctgggg aaaaacttta aatcggttat gctgcttgcc agtgcgattg
     8641 gctttttggg aatggtagca ggactttaca tttcctacta tgcagaaaca cctgcaagtg
     8701 caagtattac cattattttt gtaactgtct ttatactaat cagtttagta agacgtttta
     8761 tcaaatagga gacgaacgtg aaaaaaatta gcttattact agccagtcta tgtgccttgt
     8821 ttttagtggc ttgttccaat caaaaacagg cagatggtaa actcaatatc gtgacaacct
     8881 tttaccctgt ctatgaattt accaagcaag tcgcaggaga tacggctaat gtagaactcc
     8941 taatcggtgc tgggacagaa cctcatgaat acgaaccatc tgccaaggca gttgccaaaa
     9001 tccaagatgc agataccttc gtttatgaaa atgaaaacat ggaaacatgg gtacctaaat
     9061 tgctagatac cttggataag aaaaaagtga aaaccatcaa ggcgacaggc gatatgttgc
     9121 tcttgccagg tggcgaggaa gaagagggag accatgacca tggagaagaa ggtcatcacc
     9181 atgagtttga tccccatgtt tggttatcac cagttcgtgc cattaaacta gtagagcaca
     9241 ttcgtgacag cttgtcagca gattatcctg ataaaaaaga gacctttgag aagaatgcag
     9301 ctgcctatat cgaaaaattg caagccttgg ataaggctta cgcagaaggc ttgtctcaag
     9361 caaaacaaaa gagctttgtg actcaacacg cagcctttaa ctatcttgcc ttggactatg
     9421 gactcaaaca agtcgcaatc tcaggacttt ctccagatgt agagccatca gctgctcgct
     9481 tggcagaatt gacagagtat gtcaagaaaa ataaaatcgc ctatatctat tttgaagaaa
     9541 atgcctcaca agcccttgct aacacacttt caaaagaagc aggtgtcaaa actgatgtcc
     9601 tcaatccttt agaaagtctg acagaagagg acaccaaggc tggagaaaat tacatctcta
     9661 tcatggagaa aaacctcaag gctttgaaac aaacaacaga ccaagaaggc ccagcaatcg
     9721 agcctgaaaa ggcagaggat accaagacag tccaaaatgg ttacttcgag gatgcagctg
     9781 tcaaggaccg caccttgagt gactatgcag gtaactggca atcagtttat cctttccttg
     9841 aagacggcac gtttgaccaa gtctttgact acaaggctaa gttgactggt aagatgaccc
     9901 aggctgagta caaggcttac tatacaaaag gctatcagac agatgtgact aagattaaca
     9961 ttactgataa tactatggaa tttgttcaag gtggacaaag caagaaatat acttacaagt
    10021 atgtcggtaa gaaaattttg acttacaaga aaggcaatcg tggcgtgcgt ttcctctttg
    10081 aagccacaga tgctgacgct ggacaattca agtatgttca gtttagtgac cacaatgttg
    10141 ccccagttaa ggcagaacat ttccatatct tttttggagg tacaagccaa gaagctctat
    10201 ttgaagaaat ggataattgg ccaacctact acccagataa cctatctggc caagaaatcg
    10261 ctcaagaaat gttggcgcat taatgagaaa tcgactagtt cataagagct agtcggtttt
    10321 ttgatactag atggctaact tca
//
LOCUS       DAVBCN010000065        10219 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVBCN010000065 DAVBCN010000000
VERSION     DAVBCN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10219
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.065"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..184
                     /locus_tag="VQ497_001619"
     CDS             <1..184
                     /locus_tag="VQ497_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HET2517912.1"
                     /translation="VESFFGILKSEMFYGYEKTFKSLNQLEQAIIDYIDYYNNKRIKV
                     KLKGLSPVQYRTKSFG"
     gene            complement(312..1334)
                     /gene="cbpF"
                     /locus_tag="VQ497_001620"
                     /pseudo
     CDS             complement(312..1334)
                     /gene="cbpF"
                     /locus_tag="VQ497_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
     gene            complement(1353..2174)
                     /gene="cbpG"
                     /locus_tag="VQ497_001621"
     CDS             complement(1353..2174)
                     /gene="cbpG"
                     /locus_tag="VQ497_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HET2517913.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHNYYRHDYGKEADDIYVLPAVSP
                     SQELFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYYYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNRKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(2255..2494)
                     /locus_tag="VQ497_001622"
     CDS             complement(2255..2494)
                     /locus_tag="VQ497_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517914.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(2644..2910)
                     /locus_tag="VQ497_001623"
                     /pseudo
     CDS             complement(2644..2910)
                     /locus_tag="VQ497_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(2935..3246)
                     /locus_tag="VQ497_001624"
     CDS             complement(2935..3246)
                     /locus_tag="VQ497_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET2517915.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(3171..3413)
                     /locus_tag="VQ497_001625"
                     /pseudo
     CDS             complement(3171..3413)
                     /locus_tag="VQ497_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3414..4046)
                     /locus_tag="VQ497_001626"
     CDS             complement(3414..4046)
                     /locus_tag="VQ497_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2517916.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(4060..5055)
                     /locus_tag="VQ497_001627"
     CDS             complement(4060..5055)
                     /locus_tag="VQ497_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2517917.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FFFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVVDPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(5052..5750)
                     /locus_tag="VQ497_001628"
     CDS             complement(5052..5750)
                     /locus_tag="VQ497_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HET2517918.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(5828..6838)
                     /locus_tag="VQ497_001629"
     CDS             complement(5828..6838)
                     /locus_tag="VQ497_001629"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HET2517919.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(6822..7829)
                     /locus_tag="VQ497_001630"
     CDS             complement(6822..7829)
                     /locus_tag="VQ497_001630"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HET2517920.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLTPNPDYSLIQETIALMGDFLAVRGQNLRSFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKSEKIIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(7816..8769)
                     /gene="mvaD"
                     /locus_tag="VQ497_001631"
     CDS             complement(7816..8769)
                     /gene="mvaD"
                     /locus_tag="VQ497_001631"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HET2517921.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVIADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YSVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(8751..9629)
                     /gene="mvk"
                     /locus_tag="VQ497_001632"
     CDS             complement(8751..9629)
                     /gene="mvk"
                     /locus_tag="VQ497_001632"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HET2517922.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(9751..10080)
                     /locus_tag="VQ497_001633"
     CDS             complement(9751..10080)
                     /locus_tag="VQ497_001633"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2517923.1"
                     /translation="MTRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMIPHDFPLYLTVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVQ"
BASE COUNT         2986 a         2390 c         1799 g         3044 t
ORIGIN      
        1 ggtggagtcc ttctttggga ttctgaaatc ggaaatgttt tatggctatg agaaaacatt
       61 taaatcactt aatcaattgg aacaagctat tatagactat attgattatt acaacaacaa
      121 gagaattaag gtaaaactaa aaggacttag tcctgtgcag tacagaacta aatcctttgg
      181 ataaattatt tgtctaactt ttggggggca gtacagaaaa accttgatac tatgcgtttt
      241 ttgattgtga agacttttat ccacctatta tacaaagtta agtatccatc acagtttaca
      301 attagccttc attacttaac ccattcacca ttatagttta agtagtaacc acctactgtg
      361 gtattaacag ctaaagcacc tgaatcatag gcatagtacc agttaccatt gacttggaac
      421 cagcctgttt tcatatctcc attacttgca tttagatagt accaagtcga accttcctga
      481 taccaaccag ttgccatagc tcctgatgaa cggagatagt accacttgtt accaaggtat
      541 tgccagccag ttctcatatc accattttca gcatctagat agtaccaagt tgagccttcc
      601 ttataccagc cagttgccat agctcctgac gaatggaggt agtaccatct attacctaga
      661 aattgccaac cagtttgcat aatgccagtt gttggatcta gatagtacca tggagctgct
      721 tttagcttct cttcatcata cgtaagaggg gaatccttaa cccaaccaca tgctagctct
      781 ccaaccgcca atctgttgat gcgcgaatca aagccaccat ccttctgtaa ataataccaa
      841 tgaccctctt cgtaaatcca accagttttt aaagtatgat aactacgctg atcttcaaaa
      901 taatagactc atttctctgc tgggctatca tattcttccc catgatgttt gttggtattc
      961 gtagcagttt ttgcttctaa aacttgcttg ccaacaaatt cttgtaatac accatcttga
     1021 ccaaaataaa accaatctgg tctaagagaa atctctattc ttggagaagg accaatcgta
     1081 acccccttgt gtggagcagg tatatattgc cagccgacaa ccatctctcc ggataaagga
     1141 tcaaaataat agtacttccc atctatcact ctccagtagg tttcttttag atcccccttt
     1201 ttgtagtagg ttctaccatt ttcttggaca aactgccatc cttcagaatc atctgcaaat
     1261 actgtactgg tccctagcaa accgaagaaa aatgtggcta gtgcgacttg catcattttt
     1321 ttcaaaagct tcattttata tatcctccaa cattaaatcc actcaccaga tgaagcgaag
     1381 ttataaactt taccatcgat agtttggcta cctgtaacca ttgctccatt tgaattgaga
     1441 taataccatt ttctatttac tttttgccaa tctgtaagca tcttaccgga actgtctaaa
     1501 taataccaag tatcatttat ctcctgccaa cccgtttgtt tatcatgttg tctataatag
     1561 taccaactac catttatttt cttccatcct tcaagagagt aacctttaag aaccgaataa
     1621 ataaatggca aatttcgttc atttaattta actgcactgt taatttgatt agctccatct
     1681 cctaaagtat gcactcctac tacacggtga ctagcatcat aaactgtaga tccactagac
     1741 ccctctaaag tatcaacttg gtaatccaag aacatgccat catcacttaa aacttgtttt
     1801 ttatctgtat acatttgatg aattttaaaa ttatatgatg gatagcctgt gatagtcaca
     1861 gttattcctg tcaaattttt ttgactagta ggaagaccca aagtccctaa ttttgcacca
     1921 atgggctctt ctagaattaa taaagccaag tcatattccc ttgcatcctt agaatttaaa
     1981 tttctaaatt ccttcaaata acgaacttcc tttactttga tctttccaaa tagttcttga
     2041 cttggactaa cagccggaag aacataaata tcatccgctt ctttcccata gtcatgtctg
     2101 tagtagttgt gagccgctgt aattaacaca ttatctttaa taaaattcgc acttcccctc
     2161 ccttctacat tcattccatc ggctactcca taatacttag ataaaaccat agctgaagtc
     2221 gaatacggat actgtaaagt attatcaatt ttaatcaaat catcattacc gataatactt
     2281 ctgattgctt ttggtagtat gaaccatacg ttggtgaaat ctcagataat gaagaatcat
     2341 tagactctgg acctttttct agtgtctcac ttacctcata ttcttcacct gtactagaaa
     2401 taacactcaa agcagatact gtcgataact ggctagccaa taaagtactc gcaataattg
     2461 aaatacccaa ttttttataa acagttttct tcattattgt atcctcctaa tgtaattata
     2521 gcgtactatt ctaaatttct taatctacta tagaatcaag aaatctacca ccttctttaa
     2581 ataccctcca ttatcacata aacaggtaaa cttttcaatt aatgactgcg cttttcaatc
     2641 acgctagagg tacttacttg cttctttgat actaagttca gccattcttt ccttgttttc
     2701 atcaataaag catgttaccc aagttggatt cgttttggag tagtctcgca gaatccagcc
     2761 aatggcttta ttgataaaaa attctgtttg gttcaagtta tgaagtagaa tcttttccat
     2821 taattgagta ttggtcttct cttttcttaa caactggtgg tcaatagcga cacgtctcag
     2881 ccagatatta tctgataggc tccattttat actcaatgaa aatcaaagag caaactagga
     2941 agctagccgc agattgctca aaacactgtt ttgaggttgc agatagagct gacgtggttt
     3001 gaagagattt tcgaagagta ttaagattat ttcttctagt tcagggtgtt catacaccaa
     3061 actccctact actcgatcta ggatatctac cgtgtcccac caggattttg tcacgactaa
     3121 ctgctctagc ttaggcaaat cggtttcctt tagataagac tgcattgctt tcaaatagtt
     3181 agcagccaca tattggtatt ttctaggctc cttttcccag caagtgtctg caaaatccca
     3241 atcgataatc tttgtttttt tcgcttctag taaatatttt tatagagttt atttctttca
     3301 ggcaccgcaa tacctagaaa agaaaattga tggcgcatat aggcttccat gggccttgct
     3361 tttttagagt cttttgctgc ttctagctcc tcaagtaaat ctgctaaact catctaaaac
     3421 tcctcttgcc ccaccaaatg gtgctgaaag gcatagacag ccgcctgggt acgatcgctg
     3481 acttcaagtt tggcaagaat attggacacg tgggtcttga ccgtcttgag agagataaag
     3541 aggtcatctg cgatgcgctg attttcgtag cccttggcga tgagttggag aacatctcgc
     3601 tcacgcgcag tcaattcttc atgaagttcc atatgattgc ggtggtattc aaccttcttg
     3661 ctaacctctt gctcaatggc cagctcgcca gcagctacct tactgacggc atgaagcaat
     3721 tcatctgcac tagaagtctt gagcatatag cctttggcac cagcatctaa gactggcatg
     3781 attttttcat tgtccaaata agaggtcaca atcaaaatct tggcttcagg ccattcttta
     3841 aggattgcta aggtcgcgtc aattccattc atctcaggca tgacaatatc catgacaatg
     3901 acatctggac gcagttccaa ggccaagtca atcccttgag acccgttgga cgcctcaccc
     3961 acaacttcta catcgtcttg gaggtcaaag tagcttttca agcccaatcg gaccatttca
     4021 tggtcatcta ctagtaaaat tttcatcttt actcctttat cattccttat ctaacagggg
     4081 aatacggata tcaaccgcca gcccttgctt gggagctgtc aagagttgaa ctgttccagc
     4141 catatcttca acccgctcct tgatatttcg cagtccataa ctcaagtcgt ctaagctccc
     4201 taactggaaa ccaatcccat tgtccaccac cttcagttgc aattcaacat ctgtctgata
     4261 gaggtagaca tctaggcaag atgcctgggc atggcggagg gtattgctaa tcaactcttg
     4321 caggatacgg aagatatgct cctcgatttt cttaggcaat ttcgtcatat tctgcttgag
     4381 actaacccta agatcactct tgtcctcaag ctcttttaaa agaatttgaa tcccttctat
     4441 caagctcttc tgctccagtt caactggtcg caaatgcaag agcaaaaccc gcaaatcctt
     4501 ctgggctgtt tctaaaatag ctgtgacact ctgcaactgg gtctgcatct tttctctatc
     4561 caatttcaaa gcctgctgac tgatacccga taaaatcatg tgggccgcaa acaactcctg
     4621 actgactgta tcgtgcaaat cccgagcaat tcgcttccgt tccttctcga tgatttcctc
     4681 ttcctgagca aggctatgat tttcagcttt ttgaagagcc tctgtcaaaa ggttaagttt
     4741 acctgataag gacttgaaac tggcatccaa atctggatct acaacctgaa ccacttcttg
     4801 ccctgccaat aaacgcttga gattagcctg catttttctt agagaaagct cttcgatccc
     4861 tcgccaaaac agggctaaga gacaggtcat ggacatgctg aaaaccaaca ataaaaagaa
     4921 aaatttttct gttttttcga catcgtgcaa aaagatagac cagtcaaaat caagtatttc
     4981 cagcaagcta tgggagaaaa aaaagacaaa caggaaggag gtgagagcga taatgacata
     5041 ggcttgtttt ttcatcctct aaccacctca acatcaccaa tcatagtggt caagaaaatc
     5101 ttgacactct tgttactctt gagatagtct tttgtttctt gatgatagtg ttcattgcgg
     5161 agggctcgct tgggctggtt gaaaaaagtc aaatccccat agagacagtt aacgctgaga
     5221 ctgacttcca catctacagg tacgatgatt ttggtcgttc ctaccatctt tctgaggata
     5281 atgacattgt catgattggt taagatgact ctctccagat gaatagtgtc cttgcccatg
     5341 aagcgaaaga gattgatatc atcaaattgg caagtctggt agcttgaaaa atgatgaaga
     5401 tttccaaacc aacgattttt ctccttctta accgtcacaa cctcttcaaa aaccaaattg
     5461 gtctgctctt tttcctggtt catcatcggg taaagaagaa agagactata gataaccgca
     5521 acaaaaatag ctagaatcac aaaaggattg agcataacga tgaaaaagaa gagaatggtt
     5581 gccgctacta aaagaagatt attcccctct ttaccagtat agtagcgaat caaaagcaaa
     5641 aagaggaata gtatcagcag aaaacgcgaa aaatgctctg ataccatcaa aatcagagct
     5701 cctgtcagaa gacaggcttc gataaataaa aagattttaa attttctcat aggttcatcc
     5761 tctcccttct attttatcac aattcaaaaa agtcacctca gtctgaggat ggaaaaaaag
     5821 gcgctggtta cgcctttttc atctgatcct ttgcttcttt taattttcca taaagaagat
     5881 agtctacttt ttgtagatct gctatggtgg cacagttaag ggaacacata atcaagcgta
     5941 gatctgcttt ccagccttgg acaatgccaa tcacttcttc aactgtgtag gtttcaacca
     6001 attccagaac ggttcgtgac aatcccacag ccttagcacc aaaaaccaag cacttaatca
     6061 tatccagcgg attccgaacc cctccactaa ccaagagttc gaccttatct ttccattctt
     6121 gggcattgag aagggcctgc atggtagact gaccccattg attgaggtaa tcacgctggc
     6181 cactacgacg gttttcgata taggcaaagc tggtgccacc acgacccgat aggtccactg
     6241 tacgaacacc gaattcatag gctctttcga ttgtcttggc atccattcca aagcccactt
     6301 ccttgaggac aataggaacg ggaatttgct tgctataatc tgctagatgc gattgccagc
     6361 ttctaaactt cctttctccc tcgggcatga gtaattcctg catgacattg acatgcactt
     6421 gcaatagaac aggattcatc tcttctacag tctgaagtcc taactcgaca ggtttgtcca
     6481 atccaatatt ggttccaagg aggagattgg gatgactaga cttgacagaa aaagaatcat
     6541 ccgttggatt tttgagggct gcgctataag aacccgttac aaataaaata ccacaggatt
     6601 ccgccacctg agccagcttt tgattgattt ctcttccctt attacttcca ccagtcatgg
     6661 cattgatata aaaaggaaag tcccactttc gaccagcaaa ctctgtcgaa agatcgattt
     6721 catccagatt gtaaagaggc aaggaagaat gaatcagctc cacctcatca aagctattat
     6781 aggaactttt ctgctcaagg gcatagagga tatgctcgtc cttacgattt gtcgtcatgt
     6841 cctatccttt cttgatataa gagctcaatc cccagatcgg cccaacgatt ttttaaggtt
     6901 ttggttgatt gcgcatcaaa actcagggcg atgccacagt caccaccacc agcaccacta
     6961 ctcttggcaa cggcctgcaa atcttgactg gcttctttca actgtctaag caaaggcgtg
     7021 taaatatctg tactcaagcc ttctaaaagc ttgctggcta cttctacttg ctcgataatc
     7081 ttttctgatt tcccctgttc caaggcttcg accaaagaaa ccaccgtttc ttttgaggaa
     7141 cttaaaaaat tttgattgat attttgcttg atttgctgga ccatgtgact cgatacagcc
     7201 acttccttgg tccatcccac taagaaatca cattctaaag ttggtttcac ttgtgaaatt
     7261 gaaaagcccc aatcacgctc cagaactgtc gccaagtttt cttcttctaa ccaagcagcc
     7321 accttctggc gatcaaatga ctggtagaga accaaatcct ctgccacaat acaggcaagg
     7381 tcgcccatgg aaccattgtc tcctcgcttg agcaagacag cgctagtcag cttgaacaag
     7441 agctcctgat caacagaaac atcatacaga gccagtaaag ccttgacaac caagacaacg
     7501 acgctgccac tagaacctag accaaacttt ttcccttctc gttccatttt gccacagatt
     7561 tctagggaaa aagatcttaa attctgaccg cgaacagcga ggaagtctcc catcaaagca
     7621 atcgtttctt gaatcaagct gtagtcagga ttgggcgtta agtccactgc gaaatcaaac
     7681 atatctgaat agatacggta gctgtcagaa aaagcaatct cagccctcat atagatggga
     7741 atatccttta tcaaagctaa ctgccctggc tctaaaatag catattcacc tgcccaatag
     7801 agttttccgc aagttttaac agcaatcatc ttgactcaaa tcctttgttt ttgacacaat
     7861 caagcgataa cgctgaccga aaatttctga caaatgctcc aagtctttct cctgacaaaa
     7921 gaccttaaca ttgggaccag catccatggt aaagtagcag gcctctcctt tctcacgaag
     7981 ctggcgaaca aagtccatag cctcataaga ggcatccgtc agataagaaa aggctggact
     8041 agcagtcttt gtcgtagcat gcatagccag ggcatttttc tccgttaatt ctccaatctt
     8101 ggcaaaatca ttttccttga gataaatcag catatcctga tagtccttct cagactgacg
     8161 aacccagtcg tcaaaagtcg tcgaggtttc cacacaaagt ttcatcccgt cacggctaga
     8221 gattggtttt ttcttgtcct ctagcaccaa cataatcata gctagtttca agtctgtctc
     8281 tacagagtaa atttctccac tatccttatc ccaggctcct agcggtccat aaaaactccg
     8341 agaagaagag cctgaggcaa acttggcttc ctgcgctaac tgacttctat ccaatccaag
     8401 cttgaaataa gcattacaag ccttgaccag ggcggacaaa ccactagaac ttgaggacag
     8461 gcccgctgca gtaggcatat tgttttgagt atcgatacgg acaaagccct caccagctgg
     8521 acgataacgg tcaataatct tactcatctt ggcatgctcg acctcatttt gtagctgacc
     8581 attgatgtaa aattcgtcag ctattacatt ggctggtaaa ggcgacaagg tcgtctctgt
     8641 atacatattt tccaaagtta gagaaatact gctagtagca ggcaccatct ctttttcttt
     8701 tttctttccc caatatttga taatagcaat atttgcgtag gaacgtactg tcacaggctc
     8761 tctatccatg tctgaacagc tcctttctct tctaatcttt ctgctagttc ttgtgcgtgt
     8821 gtcaaattgg ttaccaaggc tatgatacaa cctcctagcc caccaccgct catcttggca
     8881 cccagagcac catggctaag agtcgtttca accaaaaagt ctgcctcagg gctactgact
     8941 ccaatttctt ttaaatgtaa atgcgcttga ctgaggattt gtcccagtcc ttcagcatct
     9001 ttttgtgaaa tcgcaacttc tgcttgctgg gttaattctc ccaaggcatg caaaaacggt
     9061 agggcatcct tgcccttatt ttgaaccact tggatggctt cacgagtatg accataaaca
     9121 cccgtatcgg caatcaccaa ataggcggat aaatccatct caagttctgt aaatcctacg
     9181 ttcttgataa agcgaatagg ttggtcactg agacaggtct tagcatccaa accactagga
     9241 ttcatatggg caatcatctc agctcgattg accaagattt ctagtacatc atgaggcaga
     9301 tcagcctgat agtagtcaaa taccgcacga atggccgcta tgctgatagc cgctgacgaa
     9361 cccatccccc gtttctcagg gatagccgag tcaatctcac aacgaatgca ggcttctgtg
     9421 atatccaaat actccagtga ggcataaacc gccatggaca aggtatcctc ctcataaagg
     9481 cgccaaggac tctctgcagg aactacctta caggtcacct ccacctccaa aagaggcagg
     9541 gaaatggcag gataaccgta aacgaccgca tgttccccta ttaaaattat cttactatgt
     9601 gcctgaccga caccaacttt ttttgtcatt ttttcctttt actagacgaa aaaacgtctt
     9661 atttttcata caagtattaa ttctttccta tctattttat tatattttca caaaaaaagc
     9721 gattgtttcc attcacaatc gcttctttca ttattgaacc cattcgccat tatagttgac
     9781 agaatagcca tctacggtcg tattcactgc caaggcacct gagctataag cgtagtacca
     9841 tctgccattg acctggaacc aacctgtcgt catagaacga cgaaagaaac tccataccgt
     9901 taagtaaaga ggaaagtcgt gagggatcat gcgccattga caacctgttt tagtgacgta
     9961 caaagtctca ttaacaagta ctcgtttcgg ccatttatag gtgcggtgtt tggagaaata
    10021 gggttcaatc ttagcccatt cttgatcgtt taaatcagta tcatatgctt tgcgtgtcat
    10081 aactctagtt taacattttt tgtgaataca agttctaaat aatcgaccac gaaaatttct
    10141 taagtggaaa acgccttatg aagtatgcta cgggaaactt atgcacttaa tttgacaatt
    10201 caagatgtaa aaatatata
//
LOCUS       DAVBCN010000066        10156 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVBCN010000066 DAVBCN010000000
VERSION     DAVBCN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10156
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.066"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            122..1564
                     /gene="gtfA"
                     /locus_tag="VQ497_001634"
     CDS             122..1564
                     /gene="gtfA"
                     /locus_tag="VQ497_001634"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HET2517924.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTEILPEIHEHYSIQFKIADHDYYVYDFTLPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAREVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVSF"
     gene            1702..2895
                     /locus_tag="VQ497_001635"
     CDS             1702..2895
                     /locus_tag="VQ497_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET2517925.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            3582..5561
                     /locus_tag="VQ497_001636"
     CDS             3582..5561
                     /locus_tag="VQ497_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2517926.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            5628..7124
                     /locus_tag="VQ497_001637"
     CDS             5628..7124
                     /locus_tag="VQ497_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2517927.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            7124..8050
                     /locus_tag="VQ497_001638"
     CDS             7124..8050
                     /locus_tag="VQ497_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2517928.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSIRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            8059..9126
                     /locus_tag="VQ497_001639"
     CDS             8059..9126
                     /locus_tag="VQ497_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2517929.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            9137..10063
                     /locus_tag="VQ497_001640"
     CDS             9137..10063
                     /locus_tag="VQ497_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2517930.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
BASE COUNT         2992 a         1802 c         2127 g         3235 t
ORIGIN      
        1 atatctacgg taaggcgaca ctgacgtggt ttgaagagat tttcgaagag tataacatga
       61 atttgattta cgaagccaga tcatatgaga cttggcttca attagaaaaa ggagagtaat
      121 gatgccaatt caaaataaaa ccatgttgat tacctattct gatagccttg gaaataatct
      181 taaagactta tatgataatt tggaagagca ttttggagat gctattggag gagttcacct
      241 tttaccattt ttcccatcaa caggtgatcg tggatttgcg ccagttgact acgacgaagt
      301 ggactcagct tttggtgatt gggaggatgt taagcgttta ggtgagaaat attatcttat
      361 gtttgatttt atgattaatc atatttctcg tcaatccaag tattataagg actatcaaga
      421 aaaacatgaa gccagtgaat ttaaagatct ctttttaaac tgggataagt tttggccaga
      481 aaaccgtccg acacagtctg atgtagattt aatttacaag cgtaaggatc gtgcaccaaa
      541 gcaagagatt gtgtttgaag atggttcagt ggaacatttg tggaatacct ttggtgagga
      601 gcagattgat cttgatgtga ccaaagaagt aactatggaa tttatccgta agaccattca
      661 gcacttggca agtaatgggt gtgatttgat tcgtctagat gcctttgctt atgcagtgaa
      721 gaaattggat actaatgatt tctttgtgga accagatatt tgggatttat tggacaaagt
      781 tcgagatatc gctgctgagt atgggacaga gattttacct gagattcatg aacactattc
      841 gattcagttt aaaatagcag accatgatta ctatgtttat gattttactc ttccaatggt
      901 gacactttat actctttaca gttccagaac agagcgtttg gctaagtggt tgaagatgag
      961 cccgatgaag caatttacga cgctagatac ccatgatggg attggagtag tagatgtcaa
     1021 ggatatcctg accgatgagg agattgacta tgcttcaaat gaactctata aggttggagc
     1081 caatgtcaaa cgtaagtact ctagtgccga gtataacaac ttagatatct accaaatcaa
     1141 ttcaacctac tattcagcgc ttggagatga tgatgtcaag tattttctcg ctcgtctaat
     1201 tcaagctttt gccccaggta ttcctcaggt ttactatgtg ggtctattag caggcaagaa
     1261 tgacttgaaa ttattagaag aaactaaaga aggtcgaaat attaatcgtc attactatag
     1321 caacgaggaa atagcaagag aagtgcaacg acctgttgtg aaggcccttc tcaatctatt
     1381 ttctttccgt aaccgttcag aagcctttga tctagaaggg actactgaga tagagacacc
     1441 aacagctcac agcattgtaa tcaaacgtca aaataaagat aagtccgtaa cagcagtagc
     1501 agaaattgat ttgcaaaatc agacttatcg ggtaattgag aatggagttg aagtatcatt
     1561 ttgaagcctt gatatggtgg ataaaatagg gtttttattt tggaaaacgt ttcctttgtt
     1621 ttcaaattgc taaaaaagtg gtacaatata ggatagctta ctattatctg aatcagctga
     1681 tttggagaga aaggattcat tttgaaatca ataggcttta ttgaaaagct gaaggggttg
     1741 tctagtaaag agctgatttt attgggaatt atcctaagta tctttttacc cttttatctt
     1801 tttgtagttg tactctgttt atatattatc agtttgattt ttacaggaga catgaaaagt
     1861 attcttcaga aaatggggga gcatccgatg ctgcttcttt ttcttagcta tagtactgtt
     1921 atatccattc ttgcacaaaa ttggatgggt cttgtggctt cagtaggaat gtttctattt
     1981 actattttct ttttgcacta tcagtcgatt ttatcccata aattctttcg attgattttg
     2041 cagttcgtct tgtttggtag tgtcttgtca gctgcttttg ccagtttaga acatttccaa
     2101 attgtgaaga aatttaacta tgcttttctt tcacccaata tgcaggtgtg gcatcagaac
     2161 cgggcagaag tgaccttctt taatcctaat tattatggaa ttatttgttg tttctgtatt
     2221 atgattgctt tctatctgtt tacaacgacc aagttgaatt ggttgaaagt attctgtgtg
     2281 attgcaggct ttgttaatct ctttggtttg aactttactc aaaatcgaac tgcctttcct
     2341 gctattatcg ctggagcaat tatctatctc tttacgacta ttaaaaactg gaaggccttt
     2401 tggcttagta ttggggtctt cgcgattggt ttgagtttcc tcttttctag tgatttggga
     2461 gttcgaatgg gtactttaga ctcttctatg gaagaacgca tttctatctg ggatgctggg
     2521 atggccttgt ttaagcaaaa tcctttttgg ggtgaagggc cattgaccta tatgcactct
     2581 tatcctcgga tacatgctcc ttatcatgaa catgcccaca gtctttatat tgatacgatt
     2641 ctgagttacg gaattgtggg taccatttta ttagttttgt cttctgttgc tcctgttcgc
     2701 ttgatgatgg atatgagtca ggagtcgggg aaacgtccga ttatcggcct ttatctatct
     2761 ttccttacag tggttgctgt gcacggaatt tttgacttgg ctctcttctg gattcagtca
     2821 ggctttattt tcttgctagt tatgtgcagt attccattgg agcatcgaat gttggtatcg
     2881 gacatgacgg attaagtttt ataagtttga aatcttctac cttgggtagg agattttttt
     2941 atacgatggg catgtcgtgc atctgaggtg taagtcctct gtagggatcc actaccggtg
     3001 aatacaatag cgctccccaa gcctgactat cgtgaggtag cggattaaaa tggtctgggg
     3061 atagatcgtt ttaagtctga cgcaggaaat aagaattgtc agagaaagca atagtgaaat
     3121 cgtaggctat gaatagaaac gtgatagcga ggagcatata gtggataagg tagcgagaaa
     3181 ttttaaagta taaaagatag tcttaatcta tatatgtaaa tcacaaaagt aattgaattc
     3241 ggaataaggt tggtgtgaaa agacatagta aactgaatct tgaatactag aatgatactt
     3301 ctacaatatt gttataattt aatttgaatt ccaaaatcac attgttccgt ccgtatttca
     3361 ttttactgta tgtcttccta aactccaaag tttctgcgtc aaatttccta ttttcactgt
     3421 agtctttgac acttcgaacg acaatttgaa tcatatattc aaaaaaaaat gaaaaatttg
     3481 tgccaacaag cctaaatatt ctgaaaattt gtttgatttt taatggataa tgtgatataa
     3541 tggttgtcaa tctataattc acataaacag aggagtagaa aatgaaaaaa aatcgtgtat
     3601 ttgctacagc aggtcttgtt ttattagcag caggtgtact tgcagcatgc agttcttcaa
     3661 aatcatctga ttcatcagcc cctaaagctt atggctatgt ttatacagca gacccagaaa
     3721 ccttggacta cctgatttca agtaaaaata gtacaacagt agtgacttca aatgggattg
     3781 atggtttatt cactaacgat aattacggta atcttgctcc tgcagttgca gaggattggg
     3841 aagtctctaa ggatggtttg acctacactt ataagattcg taaaggggtt aaatggttta
     3901 cctctgatgg agaagaatat gcagaggtga cggctaaaga tttcgtgaac ggtttaaaac
     3961 acgcagcaga taaaaaatca gaagctatgt atttagctga aaattcggtt aaaggcttgg
     4021 cagattatct atcaggaact tcaacagatt tttcaacagt tggtgtcaag gcggttgatg
     4081 attatacgtt acaatacact ttgaaccagc ctgaaccgtt ctggaactct aagttgacct
     4141 attctatttt ctggcctctg aatgaagaat tcgaaacatc aaaaggaagc gattttgcta
     4201 aaccaacaga tccgacatcc ttgctttata atggtccatt cttgttgaaa gggttgactg
     4261 caaaatcttc tgtagagttt gtaaaaaatg agcaatattg ggataaagaa aatgtccacc
     4321 tagatactat caatctagct tactatgatg gatcagatca ggagtcgcta gagcgtaact
     4381 tcactagtgg agcttatagt tatgcccgtc tttaccctac cagctccaac tattctaagg
     4441 ttgcagaaga atacaaggac aatatctatt acacacaatc aggctctggg attgctggtc
     4501 tgggtgtgaa tattgatcgc caaagttaca actatacttc taaaactaca gattcagaga
     4561 aagtagctac taagaaggca ttgcttaaca aagatttccg tcaagccttg aattttgctc
     4621 ttgatcgctc agcttactca gctcaaatca atggtaaaga tggagcagct ttagcagttc
     4681 gtaatttatt tgtaaaacca gactttgttt cagctggtga gaagaccttt ggtgatttag
     4741 tcgctgctca acttcctgct tatggtgatg agtggaaagg tgtgaattta gctgatgggc
     4801 aggatggttt attcaatgct gacaaggcca aggcagagtt tgcgaaagct aagaaagctt
     4861 tagaagcaga cggcgttcag tttcctattc atctggacgt tccagtagac caagcatcaa
     4921 aaaactacat atctcgtatt cagtccttta aacaatctgt agaaacagtt cttggtgttg
     4981 aaaatgtcgt tgttgatatt caacaaatga caagtgatga attccttaat attacttact
     5041 atgctgccaa tgcttcatct gaggattggg atgtatcggg aggagtttca tgggggccag
     5101 actatcaaga cccatctact tacctggata ttttaaaaac aactagcagt gaaactacaa
     5161 aaacatattt aggatttgat aatccaaata gcccttcagt agttcaagtt ggtttgaaag
     5221 aatacgataa attagttgat gaagctgcca aagagacaag cgacttgaat gtccgttatg
     5281 aaaaatatgc agcggctcaa gcatggttga cagatagttc actctttatt cctgctatgg
     5341 cttcttctgg tgcagcacca gtgctttcac gaattgttcc atttactgga gcttctgcgc
     5401 aaacaggctc taaggggtca gatgtttact tcaaatattt gaaattacaa gataaagtgg
     5461 tgactaagga agagtatgaa aaagctcgtg aaaaatggtt gaaagaaaaa gctgaatcaa
     5521 atgagaaagc tcaaaaagaa ttggcaagtc atgtgaagta aataatttct aatagaactt
     5581 tctctccatg aggagaaggt tcttttggga tttttaaagg aaataatatg aaaaaatata
     5641 tttttatgcg tgttttgcgg tcattggttt cgattttctt agtaacgact ttgacctaca
     5701 cgattatcta taccttggtt cctcgaaaat tgattttcaa gcaggatcct aactataata
     5761 aaattgcaac aacggctgat aaacgtgata actatgaaaa tactgtgttt gagcgtatgg
     5821 gctacattga gtattacgat actaaagagt tgcaagaaaa ggcaagtagc atggattctt
     5881 ctgtaacagt agaagcaaat gcgaccaata aagctattta tgaaaagtac atcaatcaat
     5941 taggtcatgg ttggactttg ggagaattta ctgaaagtgg tcaattctat gctactcgtg
     6001 aaattccaat ttttgaacgt gtttttcact tctatgctaa cttgattgac attgaccata
     6061 caaataaaat ccaagaccct gaaaatccag acttgaaacg ctaccttcgt tttgaaaatg
     6121 atccagctat cggatggtca ttggtcggtt caggaactaa acataaatat ctcttgtact
     6181 ttaacagtca gttcccattt gttcatcaaa actttgtgaa cttgaattta ggtgactctt
     6241 acccaaccta tgctaataca ccagttcttc aggttattac tcaaggtcaa ggacaaacca
     6301 aaactgccca agttcagttc ccaacaggta agaaaacgtc ttctgtaaat atttactcaa
     6361 gaacctacaa gtcacctagt caggctgact ctcgtgaagt agctagctat gggaaagatg
     6421 atccttatac agcgactgaa agtaattacc aatatccatc tatgattgtc agctctgcta
     6481 ttactggttt gattggtttg gttcttgcct atgctcttgc cgtgccactt ggttcagcca
     6541 tggctcgttt caagaacact tggattgata gcctctcaac aggggctttg accttcttgc
     6601 ttgctcttcc aacgattgcc ttggtttaca tcgttcgatt gattggatca tctattgccc
     6661 ttccagattc attccctatc ttgggagctg gagattggcg ttcttacgtt ttaccagcag
     6721 tcatccttgg tttgttgggt gctcctggta cagccatttg gattcgtcgt tacatgattg
     6781 acttgcaatc tcaagacttt gttcgtttcg ctcgtgcaaa aggtttgtct gaaaaagaaa
     6841 tttcaaacaa acacatcttt aaaaatgcca tggttccgct ggtttcagga attcctggtg
     6901 ccatcattgg ggttatcggt ggtgcaaccc ttactgaaac agtcttcgcc ttcccaggta
     6961 tgggtaaaat gttgattgac tctgtaaaag catctaataa ctctatggtc gttggtcttg
     7021 tcttcatctt tacatgtatt tctatcttct cacgtctttt gggagatatt tggatgacta
     7081 ttattgaccc acgtattaaa ttgactgaga aaggaggcaa ataatgtcta caatcgataa
     7141 agaaaaattt cagtttgtaa aacgtgacga ttttgcctct gaaactattg atgcgccagc
     7201 atattcttac tggaaatcag tgtttaaaca atttatgaag aaaaaatcaa ctgtagtcat
     7261 gttgggaatc ttggtagcca tcattttgat aagtttcatc tacccaatgt tttctaagtt
     7321 tgatttcaat gatgtcagca aggtaaacga ctttagtatt cgttatatca agccaaatgc
     7381 ggagcattgg ttcggtactg acagtaacgg taaatcgctc tttgacggtg tctggttcgg
     7441 agctcgtaac tccatcctca tttctgtgat tgcgacagtg attaacttgg ttatcggtgt
     7501 ttttgtcggt ggtatttggg gtatttcaaa atcagttgac cgtgtcatga tggaagttta
     7561 caacgtcatc tcaaacatcc cacctctttt gattgttatt gtcttgactt actcaatcgg
     7621 agctggattc tggaatctga tttttgccat gagcgtaaca acatggattg gtatcgcctt
     7681 catgatccgt gtgcaaatct tgcgctatcg tgacttggaa tacaacttgg catcacgtac
     7741 tttgggaaca ccaaccttga agattgttgc caaaaatatc atgcctcaat tggtatctgt
     7801 tattgtgaca accatgactc aaatgcttcc aagctttatc tcatacgaag ccttcttgtc
     7861 tttcttcggt cttggattac cgattacagt gccaagtttg ggtcgtttga tttcggatta
     7921 ttcacaaaac gtaacaacca atgcttactt gttctggatt ccattgacaa cccttgtctt
     7981 ggtatccttg tcccttttcg tagttggtca aaacttagcg gatgctagtg atccacgtac
     8041 acatagatag gagtagaaat gacaaaagaa aaaaatgtaa ttttgactgc tcgcgatatt
     8101 gtcgtggaat ttgacgttcg tgacaaagta ttgacagcca ttcgcggcgt ttcccttgaa
     8161 ctcgttgaag gagaagtatt agccttggta ggtgagtcag gatcaggtaa atctgttttg
     8221 acaaagacct tcacaggtat gctcgaagaa aatggtcgca ttgcccaagg tagtattgac
     8281 taccgtggtc aggatttgac agctttatct tctcacaagg attgggaaca aattcgtggt
     8341 gctaagattg cgactatctt ccaggaccca atgactagtt tggaccccat taaaacaatt
     8401 ggtagtcaga ttacagaagt tattgtaaaa caccaaggaa aaacagctaa agaagcgaaa
     8461 gaattggcca ttgactacat gaataaggtt ggcattccag acgcagatag acgttttaat
     8521 gaatacccat tccaatattc tggaggaatg cgtcaacgta tcgttattgc tattgccctt
     8581 gcctgccgac ctgatgtctt gatctgtgat gagccaacaa ctgccttgga tgtaactatt
     8641 caagctcaga ttattgattt gctaaaatct ttacaaaacg agtatcattt cacaacaatc
     8701 tttattaccc acgaccttgg tgtggtggca agtattgcgg ataaggtagc ggttatgtat
     8761 gcaggagaaa tcgttgagta tggaacggtt gaggaagtct tctatgaccc tcgccatcca
     8821 tatacatgga gtctcttgtc tagcttgcct cagcttgctg atgataaagg ggatctttac
     8881 tcaatcccag gaacacctcc gtcactttat actgacctga aaggggatgc ttttgccttg
     8941 cgttctgact acgcaatgca gattgacttc gaacaaaaag ctcctcaatt ctcagtatca
     9001 gagacacatt gggctaaaac ttggcttctt catgaggatg ctccaaaagt agaaaaacca
     9061 gctgtgattg caaatctcca tgataagatt cgtgaaaaaa tgggatttgc ccatctggct
     9121 gactaggagg aaggaaatgt ctgaaaaatt agtagaaatc aaagatttag aaatttcctt
     9181 cggtgaagga agtaagaagt ttgtcgcggt taaaaatgct aacttcttta tcaacaaggg
     9241 agaaactttc tcgcttgtag gtgagtccgg tagtgggaaa acaactattg gtcgtgctat
     9301 catcggtcta aatgatacaa gtaatggaga tatcattttt gatggtcaaa agattaatgg
     9361 taagaaatcg cgtgaacaag ctgcggaatt gattcgtcga atccagatga ttttccaaga
     9421 ccctgctgca agtttgaatg aacgtgcgac tgttgattat attatttctg aaggtcttta
     9481 caatcaccgt ttatttaagg atgaagaaga acgtaaagag aaagttcaaa atattatccg
     9541 tgaagtaggt cttcttgctg agcacttgac tcgttaccct catgaattct caggcggtca
     9601 acgtcaacgt atcggtattg cccgtgcctt ggtcatgcaa ccagactttg ttattgcaga
     9661 tgagccaatt tcagccttgg acgtttctgt acgtgcccaa gtcttgaact tgctcaaaaa
     9721 attccaaaaa gagctcggcc tgacctatct cttcatcgcc catgacttgt cggttgttcg
     9781 ctttatttca gatcgtatcg cagttattta caagggtgtt attgtagagg ttgcagaaac
     9841 agaagaattg tttaacaatc caattcaccc atatactcaa gccttgcttt cagcggtacc
     9901 aatcccagat ccaatcttgg aacgtaagaa ggtcttgaag gtttacgacc caagtcaaca
     9961 cgactatgag actgataagc catctatggt agaaatccgt ccaggtcact atgtttgggc
    10021 gaaccaagcc gaattggcac gttatcaaaa aggactaaac taataatggt tttataattt
    10081 ccatgtcaat ttttatggga attataaaac ttttttgttg gactgatttg aaaaactttg
    10141 aaagcttcct gaaaga
//
LOCUS       DAVBCN010000067         9827 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVBCN010000067 DAVBCN010000000
VERSION     DAVBCN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9827
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.067"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            275..370
                     /locus_tag="VQ497_001641"
                     /pseudo
     CDS             275..370
                     /locus_tag="VQ497_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(623..1243)
                     /locus_tag="VQ497_001642"
     CDS             complement(623..1243)
                     /locus_tag="VQ497_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2517931.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(1243..1725)
                     /locus_tag="VQ497_001643"
     CDS             complement(1243..1725)
                     /locus_tag="VQ497_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HET2517932.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1725..2567)
                     /locus_tag="VQ497_001644"
     CDS             complement(1725..2567)
                     /locus_tag="VQ497_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HET2517933.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2662..3462)
                     /locus_tag="VQ497_001645"
     CDS             complement(2662..3462)
                     /locus_tag="VQ497_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HET2517934.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3606..4985
                     /gene="glmU"
                     /locus_tag="VQ497_001646"
     CDS             3606..4985
                     /gene="glmU"
                     /locus_tag="VQ497_001646"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET2517935.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            4995..5540
                     /locus_tag="VQ497_001647"
     CDS             4995..5540
                     /locus_tag="VQ497_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2517936.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            5552..5866
                     /locus_tag="VQ497_001648"
     CDS             5552..5866
                     /locus_tag="VQ497_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HET2517937.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            5883..6575
                     /locus_tag="VQ497_001649"
     CDS             5883..6575
                     /locus_tag="VQ497_001649"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HET2517938.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNTQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            6652..7143
                     /gene="rocS"
                     /locus_tag="VQ497_001650"
     CDS             6652..7143
                     /gene="rocS"
                     /locus_tag="VQ497_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HET2517939.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7241..7831
                     /locus_tag="VQ497_001651"
     CDS             7241..7831
                     /locus_tag="VQ497_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET2517940.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGVLDLSQLFD"
     gene            7840..9603
                     /locus_tag="VQ497_001652"
     CDS             7840..9603
                     /locus_tag="VQ497_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET2517941.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         3036 a         1707 c         2092 g         2992 t
ORIGIN      
        1 aaaacacccc aaaagttaga ttttttctgt ctaacttttg gggtgtagtt catgtacacc
       61 tgatatgatg cgttttataa ttttaaagac tttttgacca gcctcatttt tttaacttga
      121 tactcagtga aaagcaaaga ttaaactagg aagctagctg taggctgctc aaagaacagc
      181 tttgaggttg cagataaaac ttgtgaggtc accaacatat ataatgtgaa gctgacgtgg
      241 cttgaataga ttttagaagg gtatgagtct ggaagtttta atggataatg caagattcca
      301 tagaatgggt aagctagagt tcttatgtga agagtttggg cataaacttt taccttttcc
      361 tccctactca tcttagtata gaaaagtgaa tctaaaatag tacataactg cttctaaaac
      421 attcttataa attgatttaa attctcaaat catattattc agttcttatt tcattttgct
      481 ctacaatcct gttgagaaga cacgtgttca tatcaaaaag gtattggcaa gttgcaatac
      541 ctttttacaa ggttcttttg tcttattttt gtttcaactg actatatctc ctatggttct
      601 agttcagaag gctaggctat aattatgatt gataagaagt atcattccaa gtattgagag
      661 tgaatgtttc aaaatcatgg gtttctataa tggtcaggct ggcatttgct agaccgccat
      721 ctttacgaag aagtggttct ttatagccta ggagagtacg aagactggca gtaagattgg
      781 cgccgtgtcc gacaattaga atacgctcag ctggactatc ttttaatgat ttgataaatt
      841 ggatggtccg ttgagttgta ctatagaggg attcggctcc gaacattcga gtgtcaaatt
      901 gagcaagatt tgaacgaaaa gcctggattt gttgcgggta aatagcttcc aaggttgcaa
      961 ttttcaaacc ttctaacttc ccaagttgcc attcacggag attaggaacg atttctaaag
     1021 aacagggggt atagagttga ctttggataa tctcagcaga tttgaccgct cgaggtaaat
     1081 cacttgaata aatctgatca aaaggaattt ccttgagata ctggccaagt cgttttaggg
     1141 tttcaatgga ttcaggaaga agaggagaat caccgctagc accttgaaaa cgaccttctt
     1201 ggttccagag ggtacgaccg tggcggacaa agtagagttt cattacttga tgtcctccaa
     1261 aatatctaca aagtctgcct ttacaaagct agccaagtct tgtggcgcga cgataatgct
     1321 gtgtccgact tcgcctgcag agacaatcat tcgatccaaa tctagagcaa ttttatcgat
     1381 aaaaatggga taattgtgtt tctgacgaat tccgacagga ttattggctc catgaatgta
     1441 accagttgtt ttttctaagt ccttttgtgg aatcatgctc acttttttat tgccagaaat
     1501 tttagctagt ttcttttcag acaagtgctg agtgataggg acaattccga taatcggtcc
     1561 ggtcttgtct cccaaaagcg ccaaggtttt gaaaatctga tctcgttcat aaccttgagg
     1621 aagctctcct tctagggcat tgatttgaat cccctgatga gggatagctg ctttagatag
     1681 gatttgttcc accaatgttt ttttgatttt aacttttttt gccattattt atatttatcc
     1741 tccaattgac tcatccaaat accaagccag attcccagcg caaagaagaa ggcgatgatg
     1801 acataaccga caagtgaaag tcctgtgtat tggatacttt cagcgtttcc tgcatttgga
     1861 attaagatca aaagggtact tgataggacg ataccgatga tgaaatgata gacgcgtgag
     1921 tggtagttgt ttaaggcata atccatcaat tttgaaaaaa cgatgagagt tgcacctgca
     1981 ccaattccaa tcggaaagaa ggttcccaag agatcaaaag ttttaaaacc agtcaacata
     2041 ggagcataga gtcccaaaat caaaagtaag ttcgatgggc tgaggccagg aaccaagacg
     2101 ccaagggcca atagtgcgcc tgctaggacg aagttaagaa agctggcgct taaggttcca
     2161 acgacaaaat ttaaggcata gagtcctaat ccagaaatga taaaggttgt ccataaccaa
     2221 gctaaatcaa tcttgtctcg gtcagattct cgagttgatt ctttgaggag gctaggaact
     2281 gtaccgataa tagctcccgc aaagctccat aatacaaaga cctgataatt ttcaagcagg
     2341 tattcaatcg ggtaggaaaa taagccgatt cccagaagca taccgatggc aactggaata
     2401 aagtacaaaa cattttcttt aaggtcttta aagggatggg ccagaaagcc aatcattcgt
     2461 tcatagattc ctaagattgc tgctagaacc cctccggaaa ttcccggtag gataaatcca
     2521 agagcaatta caatcccttt aataacgcgt gctaaccatg agagcatata tttcctccaa
     2581 ctatttctat ttcaaaaatt ccttactata ttgtatcaca atcagacaca aaaagaaaaa
     2641 gcaaatgata aacaaatgct tttaaggttt taaaaaaagt tttcgaaagg tttcttcttt
     2701 attttttaag ggagagataa cgttgatatc taaatcgtgg tcaaagccgg caatttttcc
     2761 tttagatgtg tattggtgaa tatcataatc taaatcagtt ttaggactgc tctccaaaaa
     2821 tcctgagtct gagccgtagg acggaatcca aacagaggta aacttgcctg tatcaatact
     2881 gtgttcttcc atgaagtaga caccaacgta gatgccgatg tttttagcac ctagtgatgc
     2941 tagttttgct cgaaagtttt cgacaccttc gttcatatta gacatggttt tgtcttccac
     3001 gtcaagccaa tagtaactag ggctgtaagg agaggcagca ttgtagaaaa cttcggcagc
     3061 cttttccatt tcttggacac tttttccagc tacataagcg tagacagcaa ctgggacatt
     3121 ccgcttttga agttcagtga tatgactctt ataggccttg tctattccat tgataaatga
     3181 agcatcattt tcttttgttg tttgagcacc actgtgaaca cgaacaatag cacctgaaat
     3241 attttgtgag agggcatcgt agttgatttc ctcaggacgc tgccagccag agaggtcaat
     3301 aatcggtttg tctaagtgtt tcaaagcctg tgcttcaatc tgtgctatat tggattttgt
     3361 tttaaacgat tggctgtcat taagtgggcg attgatgatt aaaatgaaca tcataatccc
     3421 aaaaaaacta aataaaataa gtggatgaat ttgttttctc atatcttata agtctaccct
     3481 aaaaatcaaa aaaaatcaaa aaaatgggtt aaggaagaga ctttagagca ttttttcatt
     3541 caagagtgcg gaatgatttg aaatatggta taataaaagg gaatttctac agaaaagaga
     3601 agattatgtc aaattttgcc attattttag cagcgggtaa agggactcgc atgaaatctg
     3661 atttgccaaa agttttgcac aaggttgcgg gtatttctat gttggaacat gttttccgta
     3721 gtgtgggagc tatccaacct gaaaagacag taacagttgt aggacacaag gcagaattgg
     3781 ttgaggaggt cttggctgga cagacagaat ttgtgactca atctgaacag ttgggaactg
     3841 gtcatgcagt tatgatgaca gaacctatct tagaaggttt gtcaggacac accttggtca
     3901 ttgcaggaga tactccttta atcactggtg aaagcttgaa aaacttgatt gatttccata
     3961 tcaatcataa aaatgtggcc actatcttga ctgctgaaac ggataatcct tttggctatg
     4021 gacgaattgt tcgtaatgac aatgctgagg ttcttcgtat tgttgagcag aaggatgcta
     4081 cagattttga aaagcaaatc aaggaaatca acactggaac atacgtcttt gacaacgagc
     4141 gtttgtttga ggctttgaaa aatatcaata ccaataacgc tcaaggcgaa tactatatta
     4201 cagacgtcat tggtattttc cgtgaaactg gtgaaaaagt tggcgcttat actttgaaag
     4261 attttgatga aagtcttggg gtaaatgacc gtgtggcgct tgcgacagct gagtcagtta
     4321 tgcgtcgtcg catcaatcat aaacacatgg tcaacggtgt tagctttgtc aatccagaag
     4381 caacttatat cgatattgat gttgagattg ctccggaagt tcaaatcgaa gccaatgtta
     4441 ccttgaaagg gcaaacgaaa attggtgctg agactgtttt gacaaacggt acttatgtag
     4501 tggacagcac tatcggagca ggagcggtca ttaccaattc tatgattgag gaaagtagtg
     4561 ttgcagacgg tgtgacagtc ggtccttatg ctcacattcg tccaaattca agtctgggtg
     4621 cccaagttca tattggtaac tttgttgagg tgaaaggatc ttcaatcggt gagaatacca
     4681 aggctggtca tttgacttat atcggaaact gtgaagtggg aagcaacgtt aatttcggtg
     4741 ctggaactat tacagtcaac tatgacggca aaaacaaata caagacagtc attggagaca
     4801 atgtctttgt tggttcaaat tcaaccatta ttgcaccagt agaacttggt gacaattccc
     4861 tcgttggtgc tggttcaact attactaaag acgtgccagc agatgctatt gctattggtc
     4921 gcggtcgtca gatcaataaa gacgaatatg caacacgtct tcctcatcat cctaagaacc
     4981 agtaggagcc tatcatggag tttgaagaaa aaacgcttag ccgaaaagaa atctatcaag
     5041 gaccaatatt taaactggtc caagatcagg ttgaattacc agaaggcaag ggaactgccc
     5101 gacgggattt gattttccac aatggggctg tctgtgtttt agcagtaacg gatgaacaaa
     5161 aacttatctt ggtcaagcag taccgcaaag ctatcgaggc tgtctcttac gaaattccag
     5221 ccggaaaatt ggaagtagga gaaaacacag cccctgtggc agctgccctt cgtgaattag
     5281 aggaagaaac agcctataca gggaaattag aactcttgta cgatttttat tcagctattg
     5341 gcttttgtaa tgagaagtta aaactatatt tagcaagcga tttgacaaaa gtggaaaatc
     5401 cgcgtccgca ggatgaggat gaaaccttgg aagtccttga agtgagctta gaagaagcga
     5461 aagaattaat ccaatcaggt catatctgtg atgccaagac aattatggct gttcagtatt
     5521 gggagttgca gaaaaaatag aggaggtcag tatgggtaaa tctttattaa cggatgaaat
     5581 gattgaaaga gctaatagag gcgaaaaaat ttcaggtcct cctttgctag atgataatga
     5641 ggaaactaag attttaccaa cctcttcttc ccgttttggt tatgccaatc ctaaggatca
     5701 tggttttagc caggaaacct tgaagattca ggtcgaacca tctattcata aaagccgtcg
     5761 tattgaaaat accaagagaa atgtcttcaa ttctaagttg aataaaatct tatttgcggt
     5821 catctttctc ttgattttgc ttgttttagc aatgaaactt ttgtaataga aaaggaattg
     5881 aaatgaaaat aggaattatt gctgctatgc cagaagaact ggcttatctg gtccagcatt
     5941 tagataatac ccaggagcaa gttgttttgg ggaataccta tcatacagga accattgctt
     6001 ctcatgaagt cgttcttgta gaaagtggaa ttggtaaggt catgtctgct atgagtgtgg
     6061 cgattttggc tgatcatttc caggtggatg cccttattaa tacgggttca gctggggcag
     6121 tagcagaagg tatcgctgtt ggggatgtcg tgattgctga caaattagcc tatcatgacg
     6181 tggatgtcac agcttttggc tatgcttatg gacaaatggc gcaacaaccg ctttatttcg
     6241 aatcagacaa aacctttgtt gctcaaatcc aagagagttt atctcaattg gaccaaaact
     6301 ggcatcttgg tttgattgct acaggagata gttttgttgc aggaaatgac aagatagaag
     6361 cgattaagtc ccatttccca gaagttttag ccgtggagat ggagggggca gctattgctc
     6421 aagcagcgca tgccctcaat ctcccagtct tagtcatcag agctatgagt gacaatgcca
     6481 accatgaagc aaacatcttt tttgatgagt ttattatcga agctggacgt cgctctgccc
     6541 aagtcttgtt gacctttttg aaggctttag attaagcgga aatttgacag tttttctagc
     6601 ttatgataag atttaagtaa agaaaagcta gaaaacgttt cagaggatat tatgagtatt
     6661 gaaatgaccg tcagtgagat tgcagaggtc ttaggattat ctcgccaagc aatcaataac
     6721 cgtgtcaaag aattaccaga agaagacaca gataaaaatg acaaaggggt aacagttgtt
     6781 accagaagtg gcttgattaa gctagaagaa atctataaaa aaacgatttt tgaagatgag
     6841 cctgtcagtg aagatgtcaa acaacgtgaa ctgatggaga ttctagtgga tgagaagaat
     6901 gcagaaattc ttcgtcttta tgaacaatta aaagccaagg atcgtcagtt atcagaaaaa
     6961 gacgagcaga tgcgtatcaa agaccgtcag attgctgaga aagacaaaca attagatcag
     7021 caacaacaat tgacccttca agccatgaag gatcaagaaa atcttaagct agagctggac
     7081 caagcaaaag aagaagtcca atccactaag aaaggctttt ttgctcgttt atttggagga
     7141 taatcaagga gctgtttagg ttaaatgcta acctgatggc ttcttttgtt tgtgaatagt
     7201 atagttgatt agaacagaga cagtagagga gaggtacaga atggaacgat tagaagatta
     7261 cattgctttt gacttagaat ttaatcagca cgaaggacaa acacacttga ttcaagtatc
     7321 ggcagtgcgt tttagagatg gtcgggaaat cgatgcctat gattcctatg ttcataccag
     7381 cgtccctcta aaaagcttta tcaatggttt gaccgggatt acagctgaga ccttaaaaga
     7441 tgctcctcca gttgagaaag tcttagaaga attccaagag tttgtgggtt ctttgctgat
     7501 gataggctac aatgctgcta aaagtgattt acctatttta gcagagcatg gtttagacta
     7561 tagccaacag tacaaggtgg atttgtatga tgaagctttt gaacgtcgga gttcagattt
     7621 acatggtatt gccaatctca aattgcaaac tgttgcctca tttcttggat ttcaaggtca
     7681 gtctcataat agtttagagg atgctcggat gacggcgcgt gtttacgaat ccttcttaga
     7741 gtcggatcaa gctagggaat tgttagagac agaaagtagc ttatcaaaca atccttttgg
     7801 tgtcttggac ttgtctcaat tatttgacta ggagtgccta tgaaacctga aaaacctaaa
     7861 aaattgggtc ttaaacagat ttacctcaat cttgataaaa tcctctttct atttttcttg
     7921 atttttatga tagtggagta tttgtgggta ccattcaatt catggttggc tagcttctta
     7981 ttagatcaga caggttatct ttttatttcc tataacaatg tcttagccat ccttactcat
     8041 tctccattga ttagtctagc ttttttagcc ttgattgtaa ttaaccttct ggttgcttat
     8101 tttcaaatag gactcctctt tattggtgct cgtcatctcc tctataatga aaagcgaagg
     8161 ctaattgagt atatccgcaa ggttttccat gaaagttttg gatttatgaa aaagctaact
     8221 tgcgctaaag ccttgtttat ctttttctat atagctatgc tttttccttt tatacggaaa
     8281 atgctgaaga tttattatct taataaaatc gtcattccag agtttatcca agcttattta
     8341 gaagataaat attggatgtg gtggctgagc atcctacttc tatctcttat ctttctctat
     8401 gtctctgtca gattgatgtt tgcccttccg aagattgtat atgatcagct gactgttcga
     8461 gaagcagtga tgtttagctt ggagaaaacc aagaagagag ttacttttta tgcttggaat
     8521 ttattttaca tcttgctcaa agcacattta ttattttatc ttcctttgat tcctttatta
     8581 ttggctcaaa ctttggtaga tgatatcact cagagagagt ctctgattct tggtattttg
     8641 aattttgtcg tgattaaaaa tctctattat atggctctga cttatttttt ggttaaattt
     8701 gtttcatttt tgacaggaaa ggagctggat atgcttccta ggagagaaaa agatcacatc
     8761 gtgcgatggg gtgtcatgac ttgtgccagt cttttctttg ccttagaagg ttatatttat
     8821 ctggaggctc ccatggttca tctacctcaa cttatttctc accgaggggt ttccaatgca
     8881 aatgggattc aaaatacagt agagtccttg gaaactacag cacaactcaa accagacttg
     8941 gtggagacgg acgtgcagga aacaaaagat gggcagtttg tcatgatgca tgatgctaac
     9001 ttgaaaaatc tagcaggtat caataaaagt cctcaagact taaacttgga ggagcttaaa
     9061 gggattgata tttttgaaaa tggctaccag actaaaattt caagctttga agattatctc
     9121 agtcgagcca acgaacttgg tcaaaaatta ctaattgaaa ttaaaaccag taaaaaagat
     9181 agtccagaca tgatgaaccg ctttttagcc cgttatgctg caaagctcaa gatttatgga
     9241 catcaaatcc agtctttaga ctaccatgtt gtcgaaaaag taagacagta tgatgccgaa
     9301 ctgccagttt atttcatcat gccctacaat tctgtctttc ctaaaacaag agcgacagga
     9361 tatactatgg agtactcaac cttggatgaa tattttgtaa gcaaactatg gacaacggat
     9421 cagaaacttt atgtctggac tgtcaatgat tcagaagcta tcagcaaatc tcttcactta
     9481 ggagtagatg gagtgattac agatgatttg gaaaaagttc agcgggaaat agaagtagcc
     9541 caagaagatc cagaatatac ggatttgctc ttgaaaaaag cattggaatt ctttgaattt
     9601 tagtaataaa aaagaggaac ggttgttcct cccatgatag gtaagtattc ttgtgaatgc
     9661 ttgctttttt gtggttgcaa tccgtttacg agaattgttt gtattgttct tgtttcaatt
     9721 tactttatag tgctttgaag ttggaatgct acactataac ttctaaaata tagtaaaatg
     9781 aaccaaaaat agtacacaat gtggtataat cttcttatgg catattc
//
LOCUS       DAVBCN010000068         9729 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVBCN010000068 DAVBCN010000000
VERSION     DAVBCN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9729
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.068"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(418..1554)
                     /gene="dnaJ"
                     /locus_tag="VQ497_001653"
     CDS             complement(418..1554)
                     /gene="dnaJ"
                     /locus_tag="VQ497_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET2517942.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(1759..2112)
                     /locus_tag="VQ497_001654"
     CDS             complement(1759..2112)
                     /locus_tag="VQ497_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517943.1"
                     /translation="MSVNKNNLSDIADYHEKIKFFEELFNLDYNPTEMLVTVERNGNV
                     IYKEFTEVAVGKSKYDHTSVFKDVMIYFSDHICQELHLYKTQDPHYNKFEFDLAKRSL
                     KITTRDNTTIFISNE"
     gene            complement(2125..3948)
                     /gene="dnaK"
                     /locus_tag="VQ497_001655"
     CDS             complement(2125..3948)
                     /gene="dnaK"
                     /locus_tag="VQ497_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET2517944.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(4428..4952)
                     /gene="grpE"
                     /locus_tag="VQ497_001656"
     CDS             complement(4428..4952)
                     /gene="grpE"
                     /locus_tag="VQ497_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET2517945.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(4979..6013)
                     /gene="hrcA"
                     /locus_tag="VQ497_001657"
     CDS             complement(4979..6013)
                     /gene="hrcA"
                     /locus_tag="VQ497_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET2517946.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFHRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTGLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLEAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(6177..6686)
                     /locus_tag="VQ497_001658"
     CDS             complement(6177..6686)
                     /locus_tag="VQ497_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HET2517947.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTTTFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            7294..9231
                     /locus_tag="VQ497_001659"
                     /pseudo
     CDS             7294..9231
                     /locus_tag="VQ497_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            9229..9729
                     /locus_tag="VQ497_001660"
                     /pseudo
     CDS             9229..9729
                     /locus_tag="VQ497_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819352.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         2862 a         1981 c         1896 g         2990 t
ORIGIN      
        1 atgacaagcc gacgctgacg tggtttgaag aaatttttga agagtatcat tcaccatttc
       61 acaactaaat cgaagacttt ctgccgtaat gaaaacacct gaaacagctt ggtttcagct
      121 gtccggaaac tttaagactt aggttcaaag tttaggtatg gaacttcgaa ggaggtcgct
      181 accgtccgtc attacttagg aaagtcttaa aaaatctata aagtaaaaag agcccgacag
      241 ctctttttgt ttatactcaa tgaaaatcaa aaagcaaact aggaaactag ccacgggctg
      301 ctcaaaacac tgttttgagg ttgtagatac gactcacata tatacggcaa gccgacgctg
      361 acgtggtttg aagagatttt cgacgctgac gtggtttgaa gagattttca aagagtatta
      421 ttctccatca aaggcatctt taatatggtc aaagaagcct tttttctttg gatttacttt
      481 caagtcacca gcagccgcga attctttcaa ggctactttt tggcggtcgt tcaagcctgt
      541 cggtgttacg acattaacag taacgtattg gtcaccaact gcaccgccac gaaggctcgg
      601 tgccccctta ctacgtaggc ggaatttctt accagtctga gttccctctg gaataaccaa
      661 ttcaacatca ccgtgaacag ttggaatatc tactgtatca ccaagagccg cttggacaaa
      721 gttgaggttg agattgtaga agatagtcgt tccttcacgt tcaaacttgt cgctagcttc
      781 tacagaaact actacataca agtccccata aggtccaccg ttaaagcctg cttcgccttg
      841 accagcgagg cgaatttgtt gacctgtttc cacaccagca gggattttca catgtacgct
      901 atgagcttgt ttctcatgac ctgttccatg acaggttgta catggatatt tgatttcttt
      961 tcctcgaccg tgacagacat cacaggttac ttggcgacgc atcataccaa gaggagtctg
     1021 cgtatcgacg ttaatgacac cagcgccatg acagcgtcca caagtgactg gacttgtccc
     1081 tggcttagca ccagagccat tacatgtacg acagccagct tcacgatgat acttaacttc
     1141 cttctcagtt ccgaagatag cttcttcaaa ggtcaaattg acacgatact ggagatcatc
     1201 tccttggcga ggagcgtttg gattgcgcga agaaccgcct ccgccgaaga aacttgaaaa
     1261 aatatcctca aaaccaccga agccacctgc cccattgaaa ccaccgaaac caccagctcc
     1321 accaaaacca ccattggcgc ctgcagcacc atactggtca taggcagcac gtttttggtc
     1381 gtcactcaaa gtctcatagg cttcttgaac ttccttgtac ttgtcctcag caccaggctc
     1441 cttgttgata tctgggtgat attttttgga aagcttacga taagcctttt tgatctcgtc
     1501 tgccgaagcg ttttttgaca cccccagacg atcataaaat tcagtattgt tcatacaaga
     1561 taccaagacc gtaaaattcg actgaaaaat aggaaatctg acgctggagc gacgctccta
     1621 gacagatttc tcttttttcc gagaatttag gtcgggttca attcctttct tttatattga
     1681 tgagataata ctccagccac tcggccctat ccctttttac cgaaaaacag aggctgagtt
     1741 gcaacctctg tggttagatt attcatttga aataaaaatg gttgtattat ctctagtagt
     1801 aatcttcaaa gaccttttag ctaaatcaaa ctcaaattta ttatagtgtg ggtcttgtgt
     1861 tttatatagg tgtaactcct gacatatatg atccgaaaag taaatcataa catctttaaa
     1921 cacagaagtg tggtcatatt tactcttacc aacagctact tctgtaaatt ctttataaat
     1981 gacatttcca tttctttcca cagtcaccag catttctgtt ggattataat ccaaattaaa
     2041 taattcttca aaaaacttta tcttttcatg ataatctgca atgtcagaaa gattattttt
     2101 attaacactc ataacactag ccccttattt ttcagtaaac tctccgtcta cgacgtcatc
     2161 gcctgcgttt cctgttgctt gtgcgccttc tgctccttct tgagcttgtt gcgctgctgc
     2221 agcttgttcg tagagtttaa cagcaagtcc ttgagctttt tcgttcaatg cttcaagttt
     2281 tgctttcatg tcgtccaagt tgttgtcttc ttgagctttc ttaaggtcat caagggcagc
     2341 ttgggcagcg tcacgttctg cgtcgaagcc tttaccttca gtttccttga ttgtcttttc
     2401 agtcgcaaag attgcttggt ctacttcatt acgaaggtct acttcttctt tacgtttctt
     2461 atcggcttca gcgtttgctt ctgcatcttt catcatgcgg tcgatttctt cgtcagtcaa
     2521 acctgagttc gattggatga caatagtttg ttctttttga gttccaaggt ctttggcctt
     2581 aacagacacg ataccgttct tgtcgatgtc aaatgttact tcgatttgag gaattccacg
     2641 aggtgcagct gggatatcag tcaattggaa gcgtccaaga gtcttgttat ctgctgccat
     2701 tgggcgttca ccttgaagaa cgtggatatc aacggctggt tggttgtctg ctgctgttga
     2761 gaagacttgt gatttagatg ttgggattgt agtgttgcga tcgataagtt ttgtaaatac
     2821 tccacccatt gtttcgatac caagtgacaa tggcgttaca tcaagaagga caacgtcctt
     2881 gacatcacca gtaatcacac caccttggat agccgcaccc atagcaacta cttcatcagg
     2941 gtttactgat ttgtttggtt ctttaccagt ttcagcttta acagcttcaa caacggcagg
     3001 gatacgagtt gaaccaccaa caaggataac ttcgtcgatt tctgacaagc tcaaacctgc
     3061 atctgaaagg gcttgacgaa ctggaacttt tgtacgttca acaaggtcac gagtcaaatc
     3121 gtcaaatttc gcacgagtca aagtcatttc caagtgaaga ggtccagcct cacctgcagt
     3181 gataaatggc aagctgattt gtgttgaagt tacaccagaa aggtctttct tcgctttttc
     3241 agccgcatct ttcaaacgtt gcattgccat cttgtcagta gacaagtcga taccgttttc
     3301 tttcttgaat tctgctacca agtggtcaat gattttttgg tcaaagtcgt caccaccaag
     3361 tttgttgtcc cctgcagttg acaatacgtc gaagacaccg tcacccaatt caaggataga
     3421 gacgtcgaat gtaccaccac caaggtcaaa taccaagatt ttttcttctt tgtcagtctt
     3481 gtccaaacca taagcaagag ctgctgcagt tggttcgtta acaatacgtt ctacttcaag
     3541 accagcaatt ttaccagcgt cttttgttgc ttgacgttga gcgtcgttga agtaagccgg
     3601 aactgtgata acagctttgg ttactttctc accaaggtag tcttcagcgt agcctttcaa
     3661 gtattgaagg atcatagctg agatttcttg tggagtgtat tcttttccat ttgcagaaac
     3721 tttttcagaa gttcccatct tagatttgat agagataact gtatctgggt ttgtaactgc
     3781 ttgacgtttt gcagcatcac caacgatgat ttctccgttt ttgaatgaga ctacagatgg
     3841 agttgtgcgg tttccttctg ggtttgcgat gattttgctt tcagttcctt caagaactgc
     3901 aactgctgag tttgttgtac ctaagtcaat accgataatt ttagacatgt gtttttctcc
     3961 ttgttagtta attttctatt tttctttttg atactcttct taagtggcga cgccgtcaga
     4021 gcttgacttc gtcaatctct ttgactaaac tttgagccta aggtctcaaa atttgcgcaa
     4081 atagcaccac ggcgaagagt atcgtctttg gttcgcttcg ctcactatta caaagccaaa
     4141 catattttat ctttcttcat agtttattgt cgtttcggac aagttttcta ttatgtaaat
     4201 tgcgacacga ggagtcgaaa tcgattttat ttcgacgacg agttagtaag gaagctaggc
     4261 aaacgtcata gcgattgccg ttttctgacg agttgcttta gctaccgtca gaattgccga
     4321 atcgaacacg ccctaagctc tgagtaaaaa agatagattt tcttgtgttc atcgaacact
     4381 tcgtcaatct cctattttta ctgcgagctt tttacgagct ttgtatctta gttatacacc
     4441 actaccattg ctgggcgtag gatgcggtca tggagtttgt agcctttttg aaagacttgg
     4501 gcgatggtat ctactgggtg ttcatcgtct gctgggagag tttggatggc catatggtag
     4561 ttatggtcaa attcgccatc tgctgcgatt tcttcaattc cttcttcttt caaagcgtga
     4621 atcaagcttt cttgcaccat ccccaagccc ttcttcacat catctgtcaa accttcaact
     4681 gcaagtgcac gctcaaggtt gtcaagagat ggtaagattg cttttgccaa gtcctggcta
     4741 cgataacgtt gcaagttttg acgttcttca ttggcacggc gttggatatt ttgcatttct
     4801 gcatgagcgc gaagatattt gttttcgaac tcatctgcac gttcatttgc caagtccaac
     4861 tcagactttt caggagttgt ttcttcagct gtttccacaa cttcctcttc ttgaacttct
     4921 tctacttctt cattttttat atcttgggcc atgttcgcct cctttaatga tttcaatctt
     4981 aatgtacttc gtaatgatta ctgctgaggt agcggtaaaa atctgtcaac ttcatggtca
     5041 aaacacggtt gaccacattg acttgattga ttagctgttg gtaatccaga ttaactggac
     5101 cgataatggc tagaattcca actccccgat aaggaatgag gaacttacta ctaatcaccg
     5161 ctaggtcagc taaacaggac tcttgaccgt ctgcaacacg aacattttgc atctgatcct
     5221 cacgcaaccc ctcacgaatc tccaaggcca ccttttgcgg ttggtcaaag aactgatagg
     5281 cttctagatt ggcaaaattc aagagattga ccttgcccgc catcacaatg ttttcgttga
     5341 acatttcctt aaagatgtgt tcaaagagat cgatgacatt atccgttgtt gtaaagtaac
     5401 gctggataat ctgcggaatc tccgtccgaa tcttgtagtg aatatctaaa acggtgtgac
     5461 cgaggaaacg ttcctgaatg atgctcttca gtttcagcaa atcctcctgc aagaagttcc
     5521 ttggaatcag aaactgacta gtaaccgttc gcgactcgtc tagggtaaat accgccaagg
     5581 ctgtatgttg ccccaaaaca acgatatcaa aggctgtcaa acgttgcctg ctcggctcaa
     5641 catccagtgc cactaccgta cagccactca ggcctgttag caagttagca gcctcttgca
     5701 gaatatcctc caatttgaag aattcctgat caaaggcttt gacaatctca tatacctcat
     5761 tttcagccag ccggtgaaaa tccagtgagt gtttcacata gtactgaaaa ccagcaacac
     5821 ttggcatccg accgcttgaa gtatgagcct tctcaagcaa cccttgtttt tctagttccg
     5881 ccatgtcatt acgaatggtt gcactgctag agttaataga ctcttgcaag gcttttgatc
     5941 cgacaggttc gtgcgttttg gtaaagatgt caataatcag atttaaaata tcctgctgac
     6001 gctctgtaac catctcatca ctcctctctg tctgatcaat tagcactcaa tgcatccaag
     6061 tgctaactta tgattctatt atacaccctt aaaagagaat gtcaagacaa aaactcaaaa
     6121 aattagcact cttttatcaa gagtgctaaa aatcaatctg aagacctaga accttgctat
     6181 tcatctactc ggtatttcct ttttagtctg aaataaccat gaattagata agaaatcaga
     6241 aaagcatata aaacaaaaat aagaaagaaa gacatagaaa gatcctctct aaacaaactc
     6301 gtacaaacaa caagaccacc taagaaagta gttgtacagg aacgaagtct agatcgcata
     6361 acttcccatt tgagcccctt actcatatag acttgatcct ctggaagtaa atcagaagac
     6421 gatttacgaa gaatcgaaca agaacctgct cctatcaatt cccaacctct atctctaaaa
     6481 tcttgcagtt catgcttata ttttttaaga aatctagaat catagatacg gtagatgaca
     6541 tcgtctggtt gacattggtc aaaatagaac aaaccaaaac gactcgttct atacctccaa
     6601 cctttcaaat gcatctcatg taaatattct tcttccttgt ccaaatcaac aatggtgaaa
     6661 atccgaaatt ctactctgct attcattgtc ttaccccaaa attagaaaac atgcctggcg
     6721 ttatttatta gataattctt tccacttttg actcaatctc caaaaaatat aagaaatctg
     6781 aatcgcaaaa actatcaata aaacccaatc tattatgaaa atcaaaaaca ctttccaact
     6841 gaaagaacta cctccagtga caaactttga gcaaaacggt agtagagcta aaaagagaag
     6901 taaaatagga agcatccgca ttgttaaaat ccgtttggca taaaaaaatc tttatttaaa
     6961 ctaaaatatt atagcaaaat ttacgccagt ttttgaacgg ctgatgtaga tattttatac
     7021 tttcaaaatg tttaaatgtg attatttatt tttgaaaaat agctcaccag cccgactgaa
     7081 agtgcttata gaatgataat aagtcgcctg ccgaaaacag cggaaaatag cggtgttatg
     7141 cggagataat ctgacgcgat gcgaaagtat attgcatact tattttcaac aatttagcag
     7201 agtattttta taagtgtgat ataatagaag tataatttgt tctgatagtt tattttatgg
     7261 agaagtagat ttttagaatg cggagggttc aatatggttg agtttataaa gtctaagaaa
     7321 gaaatgagtg aggaggatat taaagcaaat ttcatcactc ctgctattgt atccaaagga
     7381 tggaaaaatg gtgagcatat cgcttacgaa gaatacttca ctgatggtcg aattgaggtt
     7441 agaggagata aggctcgtcg taaagaagga aaaaaatcag actattcact gtattaccaa
     7501 tttggaactc gaattgcaat tgttgaggca aaggataata aacacagcgt tcgagcagga
     7561 ttacaacaag ctattgaata tggagagatt ttagatgttc catttgttta ttcttcgaat
     7621 ggtgatggct ttattgaaca cgaccgtatc acgagagaag aacgtgagct ggagttagac
     7681 gaattcccta ctcgtgaaga attattttct cgtatgacga aggaaaaagg attgacgtac
     7741 gaaattacag aagctatctc aactccatac tatacagacg ccttctcaat gaaaacgcca
     7801 cgctattatc agcaaatagc tatcaaccgt actattgaaa cagttgccag aggacaaaaa
     7861 cgagtaatgt ttgtgatggc aacaggaaca gggaaaacgt tcatggcttt tcaaattatt
     7921 catcgccttc gaaaagctgg tttggctaaa cgagttttat tcttagcaga tagaaacatc
     7981 ttagtagacc aaacgatggc tgaagacttt aagccattcg aaaaggtaat gacgaaaatt
     8041 acaccaaaac ttttgactgc tcctgaaaaa ttaaattctt tcgaaattta tctagggctt
     8101 tatcagcaac taactggtga agatggaact gaaacacatt atcaaaaatt tgacaaagac
     8161 ttctttgatt taatcgtaat tgatgaagcg caccgtggtt cagctaagga aaacagtaac
     8221 tggcgtaagg taattgatta tttcagttct gcgacacaga ttgggatgac cgctactcct
     8281 aaagaaacca agaatgcttc caatacggaa tactttggta agccaatcta tacttatagt
     8341 ttaaaacagg gaatcgagga tggttttttg gctccatatc gtgttatgag ggttaattta
     8401 gatgtggatg tggatggtta tcgtccagaa actggaaaag ttgatgctaa cggacaatta
     8461 atagaagata ggtactacgg caggaaagat tttgataaaa ccattgtcat tgatgataga
     8521 acgcaaagag ttgccaagtt tgtttctgat tatatgaagc aaaacaatgc acgatttgat
     8581 aaaacaattg ttttttgtgt tgatattgac catgccgagc gaatgcgtgc tgcacttgta
     8641 aaagagaatc tagacttagt ccaagaagac tatcgttatg tcatgcaagt aactggtgac
     8701 aacgctgaag gaaaagctca actggataac tttatggatg tcaattctaa ttttcccgct
     8761 attgtaacaa cgtctaaatt attaacgaca ggagttaatg ctaaaacatg tcgtttgatt
     8821 gttttagact ctaatatcca atccatgact gaatttaaac aaattattgg tcgtggcaca
     8881 cgtctttatc ctcaaaaggg gaaagaattt tttacgatta ttgattttcg aaatgttacc
     8941 aatttgtttg ctgaccctga ttttgatggt gagccagtga aggtgctgga aacaggtgcg
     9001 aaaacagtca gtggttctac gcctggtttc gtaggtgagg aaggtgaccc agtagaaaaa
     9061 tatatcgtta cagacaagca ggttaccatt cttaattcta ctgttcaagt attggatgaa
     9121 aacgggaaac tgattaccga aagcctgacc gactacactc gaaagaatat cttaggtagc
     9181 tacgccactt tgaacgattt tatcacagtt tggcatacgg cagataagaa acattatggg
     9241 aagtttgctg atggaagcac tcaagaaatt gatgttcctt atgatattcc tgatacttgg
     9301 gagtgggtga ggttttctac attggttgaa attgtcagag gtggctctcc acgaccaatc
     9361 aaagattatc ttacttctga agtagatgga ataaattgga taaaaatagg tgatactgaa
     9421 aagggtgaaa agtatataaa taatgttaaa gaaaaaatca aaaaatcagg gcttaacaaa
     9481 actagatttg taaaaaaagg tacatttttg ttaactaatt ctatgagttt tggtagacct
     9541 tatattttga atgttgatgg tgcaatacac gatggatggt tggctatttc gaactatgaa
     9601 aactcattaa ataaagatta cctattctat attctttcat caaatgtagt ttattctcaa
     9661 tttctatctc taattagtgg agctgttgtg aaaaacttga atagtgataa agttgcttct
     9721 attcttatc
//
LOCUS       DAVBCN010000069         9633 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVBCN010000069 DAVBCN010000000
VERSION     DAVBCN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9633
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.069"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..77
                     /locus_tag="VQ497_001661"
     CDS             <1..77
                     /locus_tag="VQ497_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2517948.1"
                     /translation="MPQSPWVERIVQVLDLDYEDLFRR"
     gene            80..667
                     /locus_tag="VQ497_001662"
     CDS             80..667
                     /locus_tag="VQ497_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2517949.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLIISLCFSLIHAQGSTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHTGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNLLVYSLLLF"
     gene            869..1006
                     /locus_tag="VQ497_001663"
     CDS             869..1006
                     /locus_tag="VQ497_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HET2517950.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
     gene            1185..3794
                     /locus_tag="VQ497_001664"
     CDS             1185..3794
                     /locus_tag="VQ497_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HET2517951.1"
                     /translation="MDYNFNLEHPFFFTNNDYSTDTSIKYQVSLPFNWHEVMNNDEWV
                     YQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICHEMRIPFKHLSTEDKFIMRNGK
                     LVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGPYILSDKRWDEAPIYLRYGVFR
                     PSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVPPDFLNKWLDKKDKKQGDFPFI
                     IDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLGFDGTYSSEKLASECKALKILN
                     EWSETPKIYWHGKIWEHTFLGIEHMKGVPLNRWVTNNFPLYEVVDKTKDYLLRVSKIV
                     EKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIIDWEQAVFSNDEKVVHKVAAPGF
                     RAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNYVSQTRIEGKKLFESLGYTREH
                     IDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIESEQDIQDFIIKLLRGFTLTYG
                     QWRKEFQSRFFPVHYYGLNFNQGIAFSDLAILWSYQQLAKKVKNFKFDDYYEIRTQVI
                     NEAVNNFKKSSLSGLFDGKIGTIWLIYEFGEIDRAVELFTTHFIEIFENSQNKNLYSG
                     QAGILLVGLYFLSKGEIDNKLGEEILIRLREYTLNYIENPETFCKVGASDVQSNDPYE
                     NFGGLLYGHAGVAWLFGEAYKLTGESIYKNGLELAVDKELVAYKVDSNNSLQYSQGHR
                     LLPYLATGSAGLLLLINRNKEILSSKYLKYLTSLERATDVVFCVLPGLFNGFCGLEVA
                     NNIYSDIDDNFSGQKKLIEQLYRYLCVIEEGFVIAGDNGLKITTDIASGFAGVAIGLV
                     SIMDNKLTILPQI"
     gene            3827..5767
                     /locus_tag="VQ497_001665"
     CDS             3827..5767
                     /locus_tag="VQ497_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET2517952.1"
                     /translation="MNQSNEIFLNTIQEKTHYKRSISSSFQELKKWNSLIFRRGRYWY
                     QFDNWSIPNTGVIKEWYVEDNDWIPKVSSSSLDNSNSLKIVFDVEKYTLPDDSILQDV
                     RISSDESYIVLVVSSLRSTNLIGIKKYTMEELFIIEDISVESSFYLGKFGVMYTRSKE
                     YGRPSKLFYKSFDSFTEEELFEENECSFRLKIVHIDSNNCFVKSVDFQKGRIFLYSFD
                     RTGFVRHSYTETVAPTPRDIALFSTEKAQYFLGLSSTEEKKDQLILRETSSGDRVSIT
                     IPYQDRARRVHCIGRYILLDCSNAANSVFYLATFKNNSLRELSVNKITFDEKTTLYEN
                     SFSDRVLLFLERRTFYEKILSFDLIENTLKTEFERPIIKSNNTKYFSKVIWTKKDSYD
                     VSIPISLFWKSEDTDELPRRKKCILSVYGAYGKNDNSDLDEIMLSIIDAGFIYAIVHV
                     RGGGYLGGEWYRSGKALNKWNSIRDFIEGVNYLRENDVIDSKRLGLITSSAGGIIAGA
                     VLNEEKNLLQSILLFSPFINPYDTLQNPNDPLSKTEIAEWGDIRDPEVKAYIKSYSPM
                     QNIEKARDSNTVIVNILGEKDPYINNNEVIEWSKKLNSIGVKSLLYLNKAAGHGGFTP
                     SDVLLMIDTLNYFFEEVGRNNL"
     gene            5764..7371
                     /locus_tag="VQ497_001666"
     CDS             5764..7371
                     /locus_tag="VQ497_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2517953.1"
                     /translation="MNYLKFIKKTKLILMGILIFLSSFNGVLLSGIIVYAGSLNQTSS
                     FSDVLRFGAISILGWSAIYISNYYLEVTEASITKDINVKIKQGYFREQYLSSEMVKDY
                     SSIISVLSNDLRLIEENYFRQIFEIISSILLFIVSLSFMLYLNFLVSIIFIVLSALPI
                     IVPVFMKKMLSNSANEYSNSNAEYTHIIKEIFNGFKTLKSYSVTKEIISLSDKKLDKL
                     EDSTFNLKRSEVLSKLVAVLISGFCFLVPLVVGCYFVIYHKSLSFSELIGIFLANDKV
                     LGPIQSIAYSLNKINTTKDLRKPFLKYLSGEKNFIDAEHDNNGLYTSSIDEIHMKDVV
                     YSITPENKLSIDFSFKSPFRVLLTGTSGSGKTTILNLINGSLKPQKGYVNLLSHGKKS
                     SDSIPTVDQTPYIFDTTIRENVTLFQNEYFSDDQIIEVLKKVNLYEELEKIDILNYQC
                     GENGSNLSGGQKQKIALARALIRNNKVYLFDEISANLDNDNSNSIHDILFNLGISFIE
                     VSHHYDLNDKRYTDIYKLENGTLFKIK"
     gene            7386..8126
                     /locus_tag="VQ497_001667"
     CDS             7386..8126
                     /locus_tag="VQ497_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2517954.1"
                     /translation="MDKDYILKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            8129..9448
                     /locus_tag="VQ497_001668"
     CDS             8129..9448
                     /locus_tag="VQ497_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517955.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASLLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVIIYQLSPFIQSCFIKLLSIIIFRNNFMFIASKWNLLYRKPY
                     IKSISAAITGAILLISSFQMISQNILSQFQDDSDLELKVAFIVYVGAPILIVLANIIS
                     IAFLSSHQERIEIQQFEILGTSNYQMVKIKVGEAIFLTFVTSLIAFLLNIKIMALIYY
                     SLEDVLIDDMNLLGLILPNFIVSIILFILIFITKSSYFIFKNAKIIS"
BASE COUNT         3286 a         1200 c         1826 g         3321 t
ORIGIN      
        1 gaatgcctca aagtccatgg gtagaaagga ttgtgcaggt attagattta gattatgaag
       61 atttatttcg gaggtaacta tgaaaagaat aattccagtt tatatattcc aacaagtaaa
      121 tgtcctattg gtatctctat acttattgaa atttctttgt atcggtgagt taactatact
      181 acagattctc tatggttcgt cactcatttc ttttttatgg atgtatggcc aacgcaaaca
      241 agcgcataag gtcaatatga aatctaggat gaaatggctt ggtgttgaat ttgttagcct
      301 actgattata agtctatgtt ttagtctgat tcatgctcaa ggaagcacga atcaagcaaa
      361 cttaattggc cttcaacatc aaattccttg gttttcattt ttattgttct taatcaatgc
      421 gagtatggtt gaagaatttc tgtatcgaga aattttatgg aatttggtca gaaaattaga
      481 tatcagagtt gctttgacaa gtgttttatt tgccttagca catcatacag gaaccattct
      541 agcttggtgt ttgtatgttt cacttgggat gtttttaggt atggtgcgct ataaatctga
      601 cttatggggc agtatgggac tacatttggt gtggaaccta ctagtttata gtttgctgct
      661 tttttaaaat aggtgacctg atttttaggt aacttctaaa actaacttcc agttttggga
      721 gagagatgga agttactttg aaaagttacg gaaacattat tttctatgtg acaacattgt
      781 ataattgctt gcattttact ggtggagaga gtataatgag ggtggataaa gtacttaatc
      841 ccaaaaattt aaataggagg agctagctat ggcagaagaa gtattaaatc ttcaattagt
      901 atctgttcaa gttgacgaaa cagatgaagt tgatggaatg cgatttagta cattcagtac
      961 aaatcgttgc ggtaactgga gtgcttttag ttgggaaaat tgttaattca tttaaattgt
     1021 aaactttaga gattctctgg agttatggat gtcgttccaa tatgtgcacg ttggaatgtt
     1081 agtgcttata tggggagaat ccaaaggatt ctcccttttt atttaaataa gtctgtctag
     1141 ctatagatat attaagtttg ataaaatttc ttggagattt tgttatggat tataatttca
     1201 atttggagca tcctttcttt ttcactaata atgattatag tacagatact agcataaagt
     1261 accaagtaag tctgcctttt aattggcacg aagtaatgaa taatgatgag tgggtttatc
     1321 aataccctat tggaaaattt gttgaaagac aagggtggaa aatacacatt agttcagagt
     1381 ataacagttc acatgaattg ttacaagatg tagcaaaaat ttgccatgaa atgcgaattc
     1441 catttaaaca tcttagtaca gaagacaaat ttatcatgcg aaacgggaaa ttggttagtc
     1501 gaggattttc tggtaaattt attacatgtt atccaaatca aaatgaatta gagtcggttt
     1561 tgcaaaggtt agaaagtgcc ttaaagcaat ataatgggcc atatatttta agcgataaac
     1621 gttgggatga agcaccaata tatctgaggt atggtgtgtt taggccgtca agggatgatg
     1681 aaaagaaggt tgctattgat gaattaattg ttggcgatga agttgttaaa gatgaacgtt
     1741 taccagtatt taaaattcca aaaggtatag tgccccctga ctttttgaat aaatggcttg
     1801 acaagaaaga taaaaaacag ggagactttc cctttattat agataatgca attagatttt
     1861 caaacagtgg tgggatatat aatgctaggc taaaagaaga tgggaaaaaa ataattttaa
     1921 aagaggctag accgtataca ggtctaggat ttgatggtac ttattcttca gagaaattag
     1981 cttcggagtg caaagcacta aaaattctaa atgaatggag tgagacgcct aaaatatact
     2041 ggcacggtaa gatctgggag cacaccttct taggaattga acatatgaag ggcgttccac
     2101 taaatagatg ggttaccaat aattttcctt tatatgaagt tgttgataag accaaagatt
     2161 atctattgag agtttcaaag atagtagaaa aactcatcga tttaacgaat aagtttcatt
     2221 ctgaaaatgt atatcatcaa gatttacatt taggaaatat cttggttaag gatgaagatg
     2281 agattagtat aatagactgg gaacaggctg tcttttcaaa tgatgaaaaa gttgtacata
     2341 aagttgcagc ccctggtttt agagcatggc gagaaacact tcccagtgag atagattggt
     2401 atggtattag acaaatagct cattatttat acatgccact agtaactaca tcggatttga
     2461 catacaacta tgtaagtcaa accaggatag aaggaaaaaa actatttgag tctttgggtt
     2521 atacaagaga acatatagat tatgtagaaa gtttattgtc atatctagat tctaaatgtc
     2581 cacagattga gaatatttcc aggaaaaaag ttttaaaacc catgcatgag attagaacta
     2641 tcgagagtga gcaagatata caagatttta ttataaaatt attgagaggg tttacgctaa
     2701 cttatggaca gtggcgtaaa gaatttcaat caagattctt cccagttcat tattatggtc
     2761 tgaattttaa tcaaggaatt gcgttttcag atttagctat cctttggagt tatcaacaac
     2821 tagctaagaa agtaaaaaat ttcaagtttg atgactatta tgagattcga acacaggtta
     2881 ttaatgaggc agtaaataat tttaagaaat cgtccctttc tggtcttttt gatggtaaaa
     2941 ttggtacaat ctggctcatt tatgaatttg gggagataga tcgggcagtt gaacttttta
     3001 caactcattt tatagagata tttgagaatt cacaaaataa aaatttatat agtgggcagg
     3061 ctggaatttt actggttgga ctgtattttc ttagtaaagg ggaaattgat aataaactag
     3121 gagaagaaat actaattagg cttagagaat atactttaaa ttatatagaa aatccagaaa
     3181 ctttttgtaa agttggcgct agtgatgttc agagtaatga tccttatgag aattttggtg
     3241 gattactata tggtcacgca ggggtagctt ggctcttcgg tgaagcttat aaattaactg
     3301 gtgaaagtat ctataaaaat ggtctagaat tggctgtaga taaagaatta gttgcttata
     3361 aagtagatag taataattca ttgcagtaca gccaaggtca tagattacta ccatatctag
     3421 caactggaag tgctggcctg cttttactta ttaatagaaa taaagaaatc ttgtcaagca
     3481 aatacttaaa atatttaact tctttagaga gggcaactga tgtagttttt tgtgtattac
     3541 caggtttatt taacggattt tgtggtcttg aagttgctaa taatatatat agtgatatcg
     3601 atgataactt ttcaggacaa aagaaattaa ttgaacaact ttatagatat ttgtgtgtaa
     3661 ttgaagaagg atttgttatt gcgggagata atggtttaaa gataacaaca gatattgctt
     3721 cgggatttgc gggtgttgca attggattgg tatcaataat ggataataaa ttgacgattc
     3781 ttcctcaaat ataatattta gataccatta ttaaggggtc atacttgtga atcagagtaa
     3841 tgaaatattt ttaaatacaa tacaggaaaa aacacactat aagaggtcta tctcttcatc
     3901 atttcaggaa ttaaaaaaat ggaattcttt gatatttagg agagggcgat attggtacca
     3961 atttgataat tggagtatac ccaatactgg tgtaataaag gaatggtatg tcgaagataa
     4021 tgactggatt cctaaggtta gttcttccag tttagacaat tcaaattcac ttaaaattgt
     4081 ctttgatgtg gaaaaatata ctttgccaga tgatagtatc ttacaggatg ttaggatttc
     4141 atctgatgag agttatatag ttttggttgt atcctcactg cgtagcacta atttaattgg
     4201 aataaaaaaa tatactatgg aagagttatt tatcattgaa gatatttcag tagaaagttc
     4261 gttctatttg ggtaaattcg gtgttatgta tactcgctcc aaagagtacg ggaggccaag
     4321 taagttattc tataaatctt ttgatagctt tacagaagag gaactgtttg aagaaaacga
     4381 atgctctttt cgattaaaaa tagtccacat tgatagcaat aattgttttg taaagtcagt
     4441 agactttcag aaaggcagga tttttctata cagtttcgac cggacgggtt ttgttagaca
     4501 ctcttacaca gaaacggtag ccccaacacc tagagatatc gctctattta gtacagaaaa
     4561 agcacagtac tttcttgggc tctcttcaac tgaagaaaag aaagatcaac taattctaag
     4621 ggaaacttct tcgggagaca gagtttctat tactatacca tatcaagata gggctagaag
     4681 agttcactgt ataggacgtt atatcctttt agactgttca aacgcagcga attccgtctt
     4741 ttatcttgct acatttaaaa ataatagttt gagggagttg agtgttaata agataacatt
     4801 tgatgaaaag acaacacttt atgaaaattc attttctgat cgtgttcttc tgtttttaga
     4861 gaggagaact ttttacgaaa aaatacttag ttttgattta atagaaaata cattaaaaac
     4921 tgaatttgaa agacccatca taaaatcgaa taatactaaa tatttttcta aggtgatttg
     4981 gactaaaaaa gacagttatg atgtaagcat accgatttct ttattttgga aaagtgaaga
     5041 tacggatgag ctcccaagaa gaaaaaaatg tattctaagt gtctatgggg cttatggtaa
     5101 gaatgataat tctgatttag atgaaattat gttatctata atagatgcag gttttattta
     5161 tgctatagtt catgtaagag gtggaggcta tttgggtggt gaatggtatc gctcaggaaa
     5221 ggcgttaaac aaatggaatt ctatcagaga ttttattgag ggagtaaatt atttaaggga
     5281 aaatgatgta attgacagta agcgattagg tttaataact tctagtgcag gtggaataat
     5341 tgctggtgcg gtgttgaacg aggaaaaaaa tttattgcaa agcattctct tattttcgcc
     5401 atttataaat ccttatgata cacttcagaa tccaaatgat cctctttcta agaccgaaat
     5461 agcagagtgg ggagatatta gagaccctga agtaaaggct tatataaagt catattcgcc
     5521 tatgcaaaat attgaaaagg cacgagactc aaatactgtt atagttaata ttttgggtga
     5581 gaaagaccca tatattaata ataacgaagt gatagagtgg tcaaagaaat taaattctat
     5641 tggagttaaa agtttgttgt atttaaacaa agcagctggt catggaggtt ttactccatc
     5701 agatgttctc ttaatgattg atactttaaa ttatttcttt gaagaagtgg gaaggaataa
     5761 cttatgaatt atttaaaatt tataaaaaag actaaactta tattaatggg gattttaata
     5821 tttctatctt cgtttaatgg tgtcttgctc tcaggaatta ttgtatatgc aggtagttta
     5881 aatcagactt cctcattttc tgacgttcta agatttggtg ctataagtat tctgggttgg
     5941 tcagcaatct atatatcaaa ttactattta gaagtaacgg aagcatcaat aacgaaagat
     6001 ataaatgtaa aaattaaaca aggatatttt agagaacagt atctttcttc tgaaatggtt
     6061 aaagattact catctattat ttcagttttg tcaaatgatt tgagattaat cgaagaaaac
     6121 tattttagac aaatttttga aataatttct tcaatattgt tgttcatcgt ctctctaagt
     6181 tttatgcttt atttaaactt cctagtttca ataatattta ttgtattatc agcattgccg
     6241 attatagtac ctgtctttat gaagaagatg ttgtctaatt cagctaatga gtactctaac
     6301 agcaatgcag agtatactca cataataaaa gaaattttta atggttttaa gacattaaaa
     6361 tcttattctg ttactaaaga aataattagt ttgtcggata aaaagttgga taaactagaa
     6421 gattctactt ttaatttgaa acgatcagag gttctttcaa aattggttgc agtactaatt
     6481 tcaggttttt gctttctagt tcccttggtt gtaggatgtt attttgtaat ttatcataaa
     6541 agcctttctt ttagtgaact gataggtatt ttcttagcaa acgataaagt tttagggccg
     6601 atacaatcaa ttgcctattc attaaataag ataaatacaa ccaaagattt aaggaaaccg
     6661 tttttaaaat acttaagtgg agagaagaat tttatagacg ctgaacatga taataacgga
     6721 ctgtatactt catcaataga tgagatacac atgaaagatg ttgtatattc tattacacca
     6781 gaaaataaat taagtattga cttctcattt aagtcaccat ttagggtatt attaacagga
     6841 acttctggta gtgggaaaac aacgatttta aatttaatta atggttcttt aaagccacaa
     6901 aaaggttatg taaatttgtt atcacatggg aaaaagagtt cagattcaat accaacagtt
     6961 gatcagacac catatatttt tgacactact attcgtgaga acgtaacttt atttcaaaat
     7021 gaatattttt cagatgatca gataattgag gtgttaaaaa aggtaaatct atatgaagaa
     7081 ttagaaaaga tagatatact aaattatcaa tgtggtgaaa atggtagtaa tttgtctgga
     7141 ggtcaaaaac aaaaaatagc tttagctaga gctctgatta gaaataataa agtgtactta
     7201 tttgacgaaa tatcagctaa tttagataat gataattcaa attccataca tgatattctg
     7261 ttcaatttag gtatttcatt tattgaagtt tcacatcatt atgacttaaa tgacaagaga
     7321 tacactgata tatataaatt ggaaaatgga acacttttca aaattaagtg attggaggca
     7381 agaatatgga taaagattat atattaaaag tgaaagggct gtatcatcaa tttttactag
     7441 gaaataataa aacgttgcaa gtgctgaaaa atgtttctct ttctgcttcg agaggagaat
     7501 ttataagtat tctaggaatt agtggttctg gaaagtcaac tttattaaaa tgtatttcta
     7561 gtttgcttga accaacaagt ggggaagtaa ttttaaatgg aatcaacccc tataaaatca
     7621 gaaatgcaaa attgtcaagt ataagacgta acgaagtatc ttttatattt caagcataca
     7681 atttaatacc ttccctgccg gtaatagaaa atatagcact tcctttgcga ttatcacaaa
     7741 aaaaattaac tattaaaaat gtagaaaact tactcaaaag aatgaagttt aatgctggct
     7801 taaacgattt tgttggaact ctgtctggtg gagaacaaca gaaggttgct atagctagag
     7861 cggttattgc tgatagtgat ataatatttg ctgatgagcc aactggggct ttagacagcg
     7921 tttctcgtga agtaattttt gaattattga gagagttagt aggggcgggt aagtgtgtaa
     7981 ttatggtaac gcacgatata gaattggcct cgaaaactga tcgtgcatta atattgaaag
     8041 atggaaaaat tttcaaagaa cttcatagac ctagcgggga agagttgtat aaaatcttag
     8101 aggtacaatc aactacggag gaatagagat gctcagatta gtttactatc aatttttaca
     8161 taataagaaa caatggttag gcgtttcacc agtaattttt gtttcgagtt tagttatggg
     8221 attggctgtt aatggggtaa ttaatgttga gaataattcg caagtatttg tcggacttcc
     8281 agatcctaaa ccaattttta tgtttccgat agtattcgga ggagtaacat tattctttgt
     8341 gctctctaac ataataaata tgttggttga aatttttaga gatgattacg aacttctgga
     8401 ggtgttggga gcaagtctcc ttcaactatc atttcttgtg gggggacaga tttttatcat
     8461 ttcctcgatt attagtttta ttgcatatct atgctctata tttgttacgt caaattatta
     8521 ttatttttta caatactttt tcggggaaaa tatattacct gatatccaat ttcaaacgag
     8581 tgctgttgga tgtatcatta cagtagtgtt gatttcattt ttagcctttc taagtggttg
     8641 cttttatacg ttcaagaaga ttagaaatag gaagagttct aaaataaggc atgttctttc
     8701 aatagtaaaa agaatactgc tattggctgg ttttagtgtt atctggttgc tttctttgca
     8761 acaaattttc caagatagca caattttagc gaaggcccag ataatattca atattgtaat
     8821 cttagatatt gtgattattt atcaattatc tccttttata cagtcatgtt ttatcaaact
     8881 actttctata attatattta gaaataattt tatgttcatt gcttcgaagt ggaatttact
     8941 atatcgtaag ccttatataa aatctatcag tgctgcaatt acaggagcta tattactaat
     9001 ttctagtttt caaatgattt ctcagaatat actatctcaa tttcaagatg acagtgattt
     9061 agagctaaaa gtggcattta ttgtctatgt aggagcacca attttgatag tacttgcaaa
     9121 tattatttca attgcatttt tgtcatcgca tcaagaaagg attgagattc agcaatttga
     9181 aattttagga acttctaatt accagatggt taagattaaa gttggtgagg ctatattttt
     9241 gacatttgtt acttcattaa ttgcattttt acttaacatt aaaattatgg ctttaatata
     9301 ctactcttta gaggatgttt taatagatga tatgaatctt ttggggttaa tacttccaaa
     9361 ttttatagtg tctattattt tatttatttt aatatttatt actaaaagtt cctattttat
     9421 ctttaagaat gcaaaaatta tttcttaaaa atttgtaagt tttcgctata taaaatagtt
     9481 aaatctattt tgtatcattg cttgcatttt actggtggag agaatataat gagggtggat
     9541 aaagtactta atcccaaaaa tttaaatagg aggagctagc tatggcagaa gaagtattaa
     9601 atcttcaatt agtatctgtt caagttgacg aaa
//
LOCUS       DAVBCN010000070         9594 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVBCN010000070 DAVBCN010000000
VERSION     DAVBCN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9594
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.070"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(<1..947)
                     /locus_tag="VQ497_001669"
     CDS             complement(<1..947)
                     /locus_tag="VQ497_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2517956.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQVIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFILVTFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTI"
     gene            complement(1027..1890)
                     /locus_tag="VQ497_001670"
     CDS             complement(1027..1890)
                     /locus_tag="VQ497_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET2517957.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTSLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(1893..3794)
                     /locus_tag="VQ497_001671"
     CDS             complement(1893..3794)
                     /locus_tag="VQ497_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2517958.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNDLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENAAV
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINCVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
     gene            complement(4222..4497)
                     /locus_tag="VQ497_001672"
     CDS             complement(4222..4497)
                     /locus_tag="VQ497_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET2517959.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(4603..5442)
                     /locus_tag="VQ497_001673"
     CDS             complement(4603..5442)
                     /locus_tag="VQ497_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2517960.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVIVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRIGRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            5584..6432
                     /locus_tag="VQ497_001674"
     CDS             5584..6432
                     /locus_tag="VQ497_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET2517961.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSGWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(6525..7442)
                     /locus_tag="VQ497_001675"
     CDS             complement(6525..7442)
                     /locus_tag="VQ497_001675"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET2517962.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            7879..8084
                     /locus_tag="VQ497_001676"
                     /pseudo
     CDS             7879..8084
                     /locus_tag="VQ497_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(8395..8688)
                     /gene="rpmA"
                     /locus_tag="VQ497_001677"
     CDS             complement(8395..8688)
                     /gene="rpmA"
                     /locus_tag="VQ497_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET2517963.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(8705..9049)
                     /locus_tag="VQ497_001678"
     CDS             complement(8705..9049)
                     /locus_tag="VQ497_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HET2517964.1"
                     /translation="MIQAVFERAEDGELRGAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(9065..9379)
                     /gene="rplU"
                     /locus_tag="VQ497_001679"
     CDS             complement(9065..9379)
                     /gene="rplU"
                     /locus_tag="VQ497_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET2517965.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYIEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(9415..9502)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
BASE COUNT         2931 a         2013 c         1613 g         3037 t
ORIGIN      
        1 aaaatcgtca taaacaattc acaaactaaa tttccagaaa aggaaagaaa agttggaagt
       61 ggtaatccca tcataaaaac tccgacacct gtcaaagcca gtaaaatcaa aagattataa
      121 atattagctt taattttact agctagaaga gccccaatga tggaaccaat agctcccata
      181 gttaaaatac ttgcataggc tccttctgac ccgtaaagct gattcgaaaa gggaagtaga
      241 aattcaaaag ctgcaaaaaa gaaattaacg ctggaagcta ccagcaaaag gaagaaaatt
      301 tcttgctgat gccagatata gtgtaaccca tccttgatat ctacaaaaat atctctccca
      361 gtaaaagcct ttttctcttg aacttttgct tcctcttttg gaaggaaagt cactagaata
      421 aaagcaatga aaaaagtcag cgagtctagc agtagcgtca tatggagact tgcaaactgt
      481 aaaacaagga aggaaagaac aggagagcta acacctacaa cctgcaaaac cagctctaag
      541 cgagaattat agatcacaat ctcatctttc tccaccactt cagttatgat agctttattg
      601 gctgtgcgag aaaaggcaaa agcaataacc tgcacaatgt tagcaacaat caaagcgcca
      661 atcatccagc tatcattcct tatgaaagaa atagccagac aaagaatccc acaaacaaga
      721 tctgccgtca ttaaaatctt acgacgagaa aaacggtctg aaataactcc gccaaaggga
      781 ttgacgagaa tagatgtgac gagctcagaa atctgataca ttcctaaaac tgtctgtcct
      841 atagtcccca tagaagccaa ccagacacta tttccataat catagagcat atttcccatt
      901 ttattgatag ccccacggct aatcaactgc actgcatagc gattcatatt aaagctcctc
      961 tcaaattttg aaactattgt atcaaaaccg aaaggagctt tttatttttt cccttatttg
     1021 ggaaaattaa cttttgacaa atttttcgta gtgttcctga taataggcta cttgctctgg
     1081 aagacctaac acatcaaaaa tatgcatggc ctcttgcatc tgcttacagc cttctttaca
     1141 ctgtcctttt tgatataagg caaaaccttt taaataatgg aaaacattac gctcataaag
     1201 cttaatacct ttgtcaataa tcttctctgt ataagcctca aaatagttgg cattataaaa
     1261 agaagaatgc tctaaacaat gctggtaaca attgagggcc aaaatcaaca ctaatctctt
     1321 atggcgacta atctcttggt aaaattcctc cctctccata acttctctac caatccgagt
     1381 gacatagtct acatcgtaga aactatagag gttaccgaaa agaatcaact catacatggt
     1441 ccattcttct gttttgaaga gataatctgc taccttatcc aaatcatcct gcttcatatc
     1501 ataactcgca tctctttgac aaatcagacc ttgtagcaaa atccagttca gctcaaaata
     1561 aagggaagtc gtcgaactct tagacttttc aagttgttct ctttgaagct tttgaaaacc
     1621 tgcaatatcg tttgaatagt aaagtgggat aatctgtgcc atcatagaca catgttcatg
     1681 attatgaaaa ttccttgcct tatccatgaa attttcgatt gttacatgaa tgttatccaa
     1741 aatctcaaag aaacgggaga ctgccaggtc agactcccca agctcaaagc gagataactg
     1801 agaggtagag caggattcgc ctgctgcttc ctttaaagaa taatttccac ttgttcgaaa
     1861 ttcacgaaat acttttccaa gatgttccat ctttacacct gatctgataa ttcttcccac
     1921 tcaagcatag cttcttcctg acgatggctg attttatcca gctcagcctg taattccatg
     1981 agtttgtcgg catcgtttgt ttccaacatt tgttcagaaa tggcttggct ttgactttct
     2041 agctcttcaa tttcagcttc tagactttcg atttgtcgca tgagtttgcg aacttctttt
     2101 tgactttctt tctgggcctg atagtcattg actggacttg cttcctttgc ttgattgcta
     2161 gttgaagctt cctcagtctg actcatttct gctgttgctt tcttctcaac atagtagtcg
     2221 taatctccaa ggtagagagt tgaaccattc tcagacaatt ccaaaacatg agttgccaca
     2281 caattgataa agtaacgatc atgactgaca aaaagcaagg taccatcaaa gtcaatcaag
     2341 gcattttcta gcacttcctt actatcaata tccaagtggt tggtcggctc atccagaatc
     2401 aaaaagttat tgttttccat agacaattta gctaaaagca aacgagcttt ttcgccacca
     2461 gatagcatgc cgactgattt tttaacatca tctcctgaga aaaggaaggc tccaagacgg
     2521 ttgcggattt caacttctgg tgtcaatttg aaatcattcc agagttcatc cagcaccgta
     2581 ttacttggtg tcagcttgct ttgggtttgg tcatagtaac caacctcaac attagcgcca
     2641 aagcgctttt ctcccttgat aaacggaatt tgatccacga ttgatttgat aaaggttgat
     2701 tttccgatac cattgggacc aacgatagca acagcattca tcttacgaag gtctaggtta
     2761 atcggttgtg acaggacttc cccatcatag ccaacagccg cattttcaac agtcaaaaca
     2821 acattgcccg acgttttttc agactggaag gtcatgttgg ctgctttctt gccagcttcc
     2881 ggcttgtcca aacgctccat tttttctagt tgtttacggc gagattgagc acgtttggtc
     2941 gttgaagcac gaactagatt gcgattgaca aagtcttcca gagcagcgat ttccttctgt
     3001 tgcttttcat agttttttgc ctcagtagct agcttttgct ccttcaactc gacaaaacga
     3061 gagtaattcc ccacatagcg atccaaggaa tgcttggtca aatctagcgt aattgtcgca
     3121 accttgtcca agaaataacg gtcgtggctg acgataatga gggcaccgct atagtttacc
     3181 aagtaattct ctagccagga gatggtttca atatccaagt ggttagttgg ctcgtccaag
     3241 accaagagat tgggcttttc aaggagcatt ttagctagag ccaaacgagt attttgacca
     3301 cctgaaagtt cagctatttt catctgccac atagactcgt caaacttgaa tccattcaaa
     3361 atcgctcgaa tatcagcttc ataggtaaag ccacctgctt ggcgaaaatt ctcagataag
     3421 cggtcataat ctgacatcag tttatccaaa tcctcaccag acttttcacc catctccagc
     3481 tccatctgac gcagttgtct ctccgtccga cgcaaatcat taaagacatg aagcatttca
     3541 tcgtagatgg tattttcaga ctcaaaacgg ctatcttggg ctaggtaaga cagagaaata
     3601 tcttttttct tattgatttc tccgctagtt ggctcctctt ctccaactaa aatcttcaaa
     3661 agagtagact tacctgcacc atttttccca acaagagcaa tccgatctcg ttcatcaacc
     3721 tgcaggttga tattatcgaa aagaacctct cctgcaaaag aacgttcaat tttattagct
     3781 tgtaaaataa tcataagagt agtatagcat gtttccctaa ggcattcaag ataatcgtaa
     3841 gtcttttagt acaactttta taacataaaa taaactaaat tatgtatatt ttatattaga
     3901 ttccttcact atcttgttgg attttctaac cagctaatct tgtttcaaat agttatcgca
     3961 caagcctatt atttaattct tttcattatt tacgtacgcg tatagcagat tgaaataaga
     4021 tgagaacaaa tcgattggga aagtaaaatt aatttctata aatgttttag caattgtttc
     4081 gtactatttt agattcagtc tactatgtac aatattttcg gaacattcaa ctttttaact
     4141 ctatttatta ctagatttca taattaaaaa gcctactgac caagctagaa agcttgatac
     4201 aataggcttt ttaaagactg attatttaac agcgtcttta agagctttac cagctttgaa
     4261 tgctggtact ttagaagctg caattgtcat ttctttacca gtttgtgggt tgcgaccttt
     4321 acgttctgca cgctcacgaa cttcaaagtt accaaaaccg atcaattgaa ctttttcacc
     4381 agctgcaaga tagtcagcta ctgctgcaaa tacagcttca actgctgctg ctgagtcttt
     4441 cttagtcaat tctgtagctt ctgctacttt agcgatcaaa tcttgtttgt ttgccatgtt
     4501 aatgattcct ccagataatt tctaattaac aaatataatc atatcctaaa atccctttta
     4561 ggtcaagtta aaaacgctat ttcttccaat tttatttatt ttttaggagt ggtaacgtat
     4621 caaaatagcc caagcgttct cacccgtgtg agtttgaata atggaacccg tttccaaaac
     4681 agaaattggc ttttcaacat aagcttgtaa gctttctttc atctcttttg cccaatcatc
     4741 actaccagaa tatgaaattc caatctctgc tacagcacgt tcagaaagcg atgttatcaa
     4801 ctcatctaac cattttttaa atgttttagt tccacgacct ttaaccattg gctgcaattc
     4861 atggtctttc atttgcatga caacacggat attgagaagg gagctcaaca agccagttac
     4921 acggccaatt cgtccacctt tgacaagatt ttccaaagtt gaaacaccaa tataaagctc
     4981 tgtatggttt ttaacctctt ctacatgaga taaaattgcc tccatatctt taccttcttg
     5041 agctaacttc gcagcctcaa caacttggaa tttcagggct tggtcagtga aggaactatc
     5101 aacaacaatc acatctgcag tagataggct agcaccttgg cgtgctgctt ctaccgtacc
     5161 cgaaagagca tgggacatat gaatagcaag aatctggcca ccatctttgc ataggtcttc
     5221 aaaaatctca gcaaagacac ctacaggtgg ctgacttgtt ttcggaagat tcttactttc
     5281 ttgcatcaac tgaagaaatt taccttcttc tttcaaatcc gcatcagaat aaacaacatt
     5341 atcaatcatt acagataatg gaacaattgt aatatctaat tgctttacta gttcaggttc
     5401 aatagtaaca gatgaatcgg ttacaatctt aatttttgtc atagtatcaa tctttctatt
     5461 ttaggattca gattggtttc cttacttcta attatatcaa aaaaaagatt aaaaatccta
     5521 atggagtcaa tcaaattttc cgtaaaattt gatataatca acttataaga aaagaggtgt
     5581 cctatgatta aaaaaattta ccccattttt accattttac taggtgctgc tatttatgct
     5641 tttggactga cttattttgt agttccccat catctctttg aaggaggggc gacaggtatt
     5701 accctcatca cattttatct ttttaaaatc cctgtttccc tcatgaacct gctgattaat
     5761 attccccttt tcatcctagc ttggaagatc tttggagcca aatccctcta ttctagttta
     5821 ctaggaacct tagctttgtc cggctggtta gctttttttg agcatattcc ccttcatatt
     5881 gatcttcaag gtgatttact aatcacagcc cttatagcgg gaatcctatt gggaattggc
     5941 cttggaatta tttttaatgc tggaggtaca actggcggaa ctgatattct agctcgtatt
     6001 ctcaacaaat acactcatat atccatagga aaactgctct ttatcttaga tttttgtatt
     6061 ctcatgttga ttctcctaat cttcaaggat ttgagattgg tttcctacac gcttttgttt
     6121 gattttattg tttctcgtgt tattgatttg attggtgaag gaggatatgc cggcaaaggc
     6181 tttatgatta tcacaaaacg tcctgaccaa cttgctaagg cgattaatga tgacctcgga
     6241 agaggtgtta cttttatttc tggtcaaggc tactatagta aagaaaattt gaaaatcatc
     6301 tactgtattg tcggaagaaa tgaaattgtg aaaacgaagg aaatgattca tcgaatcgat
     6361 cctcaagcct ttataactat tacagaagcc catgaaatcc taggagaagg cttcaccttt
     6421 gaaaaagaat aaaaagaggt aatgtcgtga cctcaaaagt tagactaaat catctatctt
     6481 ttgggttaca gacaacctct tttttgtttt atttactcaa gctcttaaga ccaattccga
     6541 gttacttctt catcagcctt taactgatcc actaattggt caactgagtc aaatttggtc
     6601 atatcacgaa tgcgatcaag ccaataaacc atgacggttt ccccataaat atcttgatta
     6661 aaatcaaaaa tattgacttc aaaacgtgct tcttctccat caaaggtcac atttttcccg
     6721 acactagcca tagcacgata cttctgtctt tgaatctcaa catcaacgac ataaacgcca
     6781 tctgctggca tataagtacg gtctaaaagc actaaattcg ctgtcggata accaattgta
     6841 cgaccacgag cattaccatg aaccaccata cctcttgatg gaagcggtgc ccccaaaagt
     6901 tttcctgctt ctttcacatt tccatctaaa atagcttgac ggatacgagt tgaactaatc
     6961 tttcctttct catcttctac aggtggaaca atgataactt ctccatcaaa gtaatccttt
     7021 aaatcttctg ctgttttttt gtcagaacca aatgtataat caaaacctgc aacaataatt
     7081 ttggcattca tagccttgat ataagttgca aagaattctt gtgcagtgag actagcgaat
     7141 tgactactaa aatcaaggag atataattct tctacacctt cgcgctttaa ttttctttca
     7201 cgttcagcag ggttcaaaat atgcaaaaac aaatccggat gataaggctc taaagcgatc
     7261 tttggagatt cattaaaggt cataacgacg ataggtaata aatcctttct cgcagccttg
     7321 ttggcaacac gaaataattc ttgatgcccc ttatgtatgc catcaaaata gccgagaaca
     7381 acgactgaat cagatggtgt gccaatatct ttttggtttt ttataggaat agtaataatc
     7441 ataaaataat tatatcatag cgatagctat ttctggaaca gaaaatctga aatgttgttt
     7501 ttttcacatg aagtgtacct gttttcaaaa agcactttat tctatcgttg cttaactatg
     7561 aactttgcaa tattcttctc aaaaacttgt aggacatctt caaaattttg caaggagtga
     7621 ttagacttgt tcggtaacca taaagtgtca tactatgtta tgtatgaaaa agcaatgcaa
     7681 ctaactcctg agaactttaa attactaatt ggtgccgaaa aggtagaatt tagaatcgag
     7741 gtacacctat ggctgtaaaa tttacaaaat gagacaactt gggcaagatg tttgaagaat
     7801 ttcctaaact ccctgatttg aagcaagtca ctttccctga tgacaaagaa aaaagccaaa
     7861 acagcaaaga aaaactagat gactgctttc caacaactcc catctagtgt gcttcagact
     7921 gggctatttt tctctccatc tgttagcttg gattctcaga ccgtttcagc taaagaatat
     7981 cttttccctt atcagaagga acggctcaaa ccattcagac aagtgaaggg acgacaagcc
     8041 aatatttgaa accagatagc agttcttata gtcaattgaa ataaaatctg aagaaatcga
     8101 gtaggaaact catatcaatt tttaacagtg ttctattcca gattcatact caatgaaaat
     8161 taaagtgcaa actaggaagt tagccgcagg ctgtacttga gtacggcaag gtgaaactga
     8221 cgtggtttga atttgatttt cgaagagtat caatccacta tacatattaa aaagtatgga
     8281 tatcaaaaca aaaaaacagg ctctccgaaa actcggaaag ccttatttta agctgtttct
     8341 agcttcctca cctttacatt tcaaggttcg ggataaaagg ttcaatggac ctttttattt
     8401 agcgattggg taaacagaaa cttgtttttt atcgcgtcct ttacgttcaa agcgtactac
     8461 gccttcaact ttagcgaaca aagtatcgtc tccaccacga ccaacgttta cacctggata
     8521 gatgtgtgta ccacgttgac ggtaaaggat tgatccacct gttacagttt gtccgtcagc
     8581 tgctttagct ccaagacgtt ttgcttgtga atcacgtccg tttgatgtag aacctccacc
     8641 ttttttgtgg gcgaaaagtt gcaagttgtt aagagtcatt tttaacataa tgttttcctc
     8701 cgtgttagtt ttctgtgata actctggttt ggacgaactc agaatagttc tccgataagt
     8761 ttgccatacc taagaaaaat gattcaaaga ataactgggt catttctctc tggtgtgaag
     8821 gaagatcttt tggtatttca accatcagat agccaccttc atcttcgttt aattctagga
     8881 ttggttcata gcctgcaaat ttctcaatag aattgataaa gttaatggca agcgtagaaa
     8941 ccgatgcaca cacgacatct aagccgtatt cgccactctc ggcgtgtcca gtaatttccg
     9001 cacccctcag ctcgccatct tcggctctct caaagactgc ttgtatcatg tgttctcctt
     9061 aaaattaagc gttaattgcg ttgatgacaa cttttgtata tggttgacgg tgaccttgtt
     9121 tacggtggct acctttttta ggtttgtact tgtaagtaac aactttcttt tgttttcctt
     9181 gtttttcaac agttccaact acagtagctc cagcaacaag tggagttccg acaacagtgt
     9241 tttcaccacc aacaagaaca acttcgttaa aagtaacttc ttgaccagct tcaacgttca
     9301 atttttcaat gtaaactgct tgaccaactt caactttaac ttgttttccg ccagttttga
     9361 taattgcgta tgtgctcatt atgcacctcc tatgattttt atgggtttcc ccgatttttt
     9421 gtgaagactc gcctagcatc gtgggacaaa ccacttaaac ttgaataatc aagcaacgat
     9481 gttcgtgcgg ttgcacagga tcgtgcatag tcaactcttc aagtatagca tatctcctat
     9541 tttcttacaa gtaataacac ctaaaatcaa gctttttctt ttactttttt ctgc
//
LOCUS       DAVBCN010000071         9406 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVBCN010000071 DAVBCN010000000
VERSION     DAVBCN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9406
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.071"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            168..854
                     /locus_tag="VQ497_001680"
     CDS             168..854
                     /locus_tag="VQ497_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2517966.1"
                     /translation="METKKIKNLKGQIIVSCQALEGEPLYTPNGGVMPLLAKTAFQAG
                     AKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILAL
                     DCTNRSRPGYDNITDFIHDIKVKYPNQLLMAAISTFEEGKVAAESGVDFVGTTLSGYT
                     PYSPKKDNPDFELVERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVGGAITRPL
                     EIAKKFIEVV"
     gene            855..1787
                     /locus_tag="VQ497_001681"
     CDS             855..1787
                     /locus_tag="VQ497_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2517967.1"
                     /translation="MNQEILKKSKIQLTVEHIQKYWILYLMMIPATVLLILFTYGPMY
                     GIIMAFQDFTVFKGYTGSPFVGLKHFQRLFSDPLFYRLFKNTFMVGVLDFLFFFPAPL
                     IFALILNEVRKVRFKSVVQSISYLPHFIPLVVMVGIIFELFGSYGIINSLLSSLGMEP
                     ISFFTKSEWFLPLYIGSGVWKTIGWGSIIYMGALTNIDSTLYEAADMDGANRWHKMWH
                     VTLPSLRPTVVTLFILNAGGIMQVGFEKVFLMSSPATYEVSDVLSTYVYRQGILNSDF
                     SYSAAVGLFNNIVALLFVLLANKIAKKLGEEGIV"
     gene            1798..2727
                     /locus_tag="VQ497_001682"
     CDS             1798..2727
                     /locus_tag="VQ497_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2517968.1"
                     /translation="MIFSKNTKKSNRDKETFVDYLFIFILIIITLLMLYPLWWVVMTS
                     LSEPVWLSKNVVKLFPLKIDITAYKFIFGDSKLWQAYGNSVLYAALSTIVSVAVCSMY
                     AYPLTIPEIRGKKFWNTLILIPMFFSGGMIPLYIQVANLGLLNTAWALVLPGAVSTYY
                     IILFRTQFKSIPMELRESARIDGASEFRIFWNLVLPLSKVIFMIIALYCIVGSWNSFT
                     PPLLYLNDDTKMPLSIYLRNLVVTNTTDTAELSLSAGNTYYQQIAAQGGGIGMRIAIK
                     MGTIVVSVIPIMMIYPFIQKHFVKGILLGSVKG"
     gene            2741..4180
                     /locus_tag="VQ497_001683"
     CDS             2741..4180
                     /locus_tag="VQ497_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517969.1"
                     /translation="MQNLKKYLLLDSGLVVGYTLENAQLKLNKLKKDTENNPLFGEEY
                     FAENPKLWEVRFDNSYPNVIYDKELKLYRCWYQTFVSDEASEETPLAERGEKEYIVKS
                     SRMTALCYAESKDGVKWEKPNLNLVKFKGSKDNNIVLMHAHGAGIMLDEQELDPKKRY
                     KLVSKLEFSPNLHYMVVVFSEDGIHFDEPIPWKKWNPAADSHNFPFRDPKTGKYAVIT
                     RTWANNVRLSAITFSDDFINWSEPKEILRGDGFEDQIYSMPVISYADIYLGFASMYHE
                     GDTLNENYDKVDLELKFSANLEQWDSVCKGDYLIERGGGVYPQNADFDASCIFTAAPI
                     IEDDKIWFYYMGGNGNHTGFRESSLARGYIKKDQFAYYTNKREGIPAKISTVSFQTYD
                     DEIYILADIPENGYIKVAVGTKYGNAYSDFGQEDISLVQVSDGKYKLEFNKKSILDLG
                     NSPVAFKFEIENAKLYGIEGSIQQHYLKY"
     gene            4249..5826
                     /locus_tag="VQ497_001684"
     CDS             4249..5826
                     /locus_tag="VQ497_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2517970.1"
                     /translation="MVKLNKVLKGSVAFLSASLLLAACGNNANSKPAEKTADGKTKIS
                     FLSTWQINDVRPPKDETLVGQWLEEQLGIDIDLVTIPGEGVSSKVNSLITSNQLPTVL
                     LTTGDKSDIAGINKLGKQGAFLDLSQHMDKLPNYKKYLEKNNALAQIEDDEKHIYAFT
                     KFFTDENIMYTTPILRKDLLVGSEFEDLSKIKTVDDYTKVLKYLTEKQGSPAFIQRNG
                     YEGFMKRVTPLWNLSHRTYYDYESDSYKHPVEQPQLKQFVEWLKELRKDNVLHPDWAV
                     MKDETWEGLLASNKGSFTVDRMNIIGDNNFDKSFDWQPLVYPEINGKRYLQPKTPYIS
                     DSGWVINANAPKEEIDKALEFFNFLYADENQEKLAIGFEGKTFTRENPNTQAGIKWLI
                     QIYGENAGNDKAELLFKHGNQAFTRVQTKLDIEAQPGTFPKVMYDQVDKIKKDLGGFR
                     PDTPVLSFTTEELDVLTKSEAGLNTFFDENIIKFIEGQRDMSEWDSFINEAKSKDLDK
                     VTKVYNDAYKRVKDRMK"
     gene            5886..7346
                     /locus_tag="VQ497_001685"
     CDS             5886..7346
                     /locus_tag="VQ497_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET2517971.1"
                     /translation="MYDVVIIGAGSSGALAAIASGRLGLKTCLIEKGSRIGGVPINTL
                     MGSFANLYFDDSGRVNASKIVGELIERIIKKGGTVFNSMEELMNVNDFYQITIPYQPE
                     VYESVLTDMLIESKVDILLNVEVNNIIYDSKNIKSLEILSNQEVKLIDSKVFIDATGN
                     ATIASASGADVYEKNSSYGCLMRVGGVDISKTIEHIEKTKQWNYIEGYNQWLEEVLAN
                     RKYRATGAVLKDPVSYDHAPMLSKDDHFMNDEKWNYIKERWEKFRFIYTLEVSLFRNE
                     LKKAVESGDFNFEIYHDDKSGVTMNGDGISYGSWGQNVALINVAKAFGFNPKYMEDES
                     HANILSRKYNLTFLSFLKKYVPGFENSFILDQGTRATNRSCRHIDNSSMMDEILDFPM
                     YTFKTMYSYQTPKEITYKSIVGGKLNNLFVIGKGAYQSESFRSQISCMLMGISSAAAA
                     KTIIEDKSNTLNINRDLFKSNLDILFTWEFSNWITD"
     gene            7368..7823
                     /locus_tag="VQ497_001686"
     CDS             7368..7823
                     /locus_tag="VQ497_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET2517972.1"
                     /translation="MNDTNKNQYFVYEFDTSFVEMSYSQFFEGYCILFSKEPYPSLSD
                     MPMEFRKEFLMEMSILGDAMLEVLNAYRINYDILGNSTPVLHAHLFPRYMWEEEERRK
                     TVVHRYDSSVFSNPEFEFTIEKHGPIIKKLRSAIDRHYREYQQLLDDSN"
     gene            7910..8315
                     /gene="cdd"
                     /locus_tag="VQ497_001687"
                     /pseudo
     CDS             7910..8315
                     /gene="cdd"
                     /locus_tag="VQ497_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221146.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
     gene            8328..8777
                     /locus_tag="VQ497_001688"
     CDS             8328..8777
                     /locus_tag="VQ497_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2517973.1"
                     /translation="MELVEFKNGHVNTVHEHIAITKVKEFLNNHDILKDKGGIHKIST
                     DFFYNIIDMETTTEENRPWESHKEYYDVHYLLNGEETILYNFLSQMELSEYKVDDDWQ
                     QMNGTALFSIKLKKDMLLLLEPNDAHRTGLLVEEPLNIKKVVFKVKI"
     gene            complement(8889..9406)
                     /locus_tag="VQ497_001689"
                     /pseudo
     CDS             complement(8889..9406)
                     /locus_tag="VQ497_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         3247 a         1270 c         1754 g         3135 t
ORIGIN      
        1 gtgtattgaa tctataacag tacgccttaa atgctaaaat atttctataa attaatttaa
       61 ctttcctgat agggctgttc atatcttatt tcaattcact atataaataa aattgtagta
      121 gatgttattg aattagtaaa aaaaataaaa aaacggaggt tttctgaatg gaaactaaaa
      181 aaattaaaaa tttaaaaggt caaataattg tttcctgcca agcgttagaa ggagagccac
      241 tttatactcc aaatggagga gtgatgccgt tattagcaaa aaccgccttt caagcaggcg
      301 ctaaaggtat tcgtgctaat tctgtccgcg atatttcaga aataaaagaa gaagttgact
      361 taccaattat aggtatcata aaaagagatt acgatggctt tgaacctttt atttcagcaa
      421 ctatgaaaga gattgatgaa ttagttagtg aaggagtaga cattttagct ttggattgta
      481 ctaatcgttc aagaccaggt tatgataata ttactgactt tattcatgat attaaagtca
      541 agtatccaaa ccaattatta atggctgcta tttctacttt tgaagaaggg aaagtagctg
      601 ctgaatcggg agtagatttt gtgggtacga cattatctgg ttatacacca tatagcccta
      661 aaaaagataa tcctgatttt gagttagttg aaagattggt aaaagagctt gacgtaccag
      721 ttattgctga aggaagaata agtactccgg aacaagctag aaaaatgtta gatttaggtg
      781 cttacgcagt ggttgttgga ggagctatca ctcgtccttt ggaaatagcg aagaagttta
      841 ttgaggtagt ttagatgaat caagaaatat taaaaaagag taagattcaa ttgacagttg
      901 aacatattca aaaatattgg attttatatt taatgatgat tccagcaact gttctactaa
      961 ttttatttac gtatggacca atgtacggca ttattatggc ctttcaagac tttactgtct
     1021 tcaagggata tactggaagt ccatttgtcg gtttaaaaca ttttcaaaga ttgttctcag
     1081 atcctttgtt ttatagattg ttcaagaata cgttcatggt aggggtttta gattttttat
     1141 tctttttccc agctccgctt atttttgcac tcatattaaa cgaagtcaga aaagtaagat
     1201 ttaagagtgt agttcaatct attagttatc ttcctcattt tattccccta gtggttatgg
     1261 ttggtattat ttttgaacta tttggttcat atggtattat taacagttta ctttcatcac
     1321 ttggaatgga gccaataagt ttctttacaa aatctgaatg gttcttgcct ctctacattg
     1381 gttcaggagt atggaaaaca ataggttggg gttctatcat ttatatggga gcattaacca
     1441 atatagattc aactttatat gaagcagcag atatggacgg ggctaataga tggcacaaaa
     1501 tgtggcatgt aactctacca agtttaagac ctacagtagt tactttgttt atactaaatg
     1561 ctggtggtat catgcaagta ggatttgaaa aggtattctt gatgtctagt cctgcaacat
     1621 atgaagtatc tgatgtatta tctacttatg tttatagaca aggtatttta aattctgatt
     1681 tcagttactc agctgcagta ggattattta ataacatagt agctttgtta tttgttttat
     1741 tagctaataa gattgctaaa aaacttggcg aagaaggaat tgtataaggg gggtaaaatg
     1801 attttttcaa aaaatacaaa aaagagtaat agagacaaag aaaccttcgt tgattatctc
     1861 tttatattca ttttaataat aataacctta cttatgttgt atccattatg gtgggtagtg
     1921 atgacgtcac tttcagaacc agtttggcta tctaaaaacg ttgttaaatt atttccctta
     1981 aaaatagata ttactgcata taagtttatt tttggggatt ctaaactatg gcaagcatat
     2041 ggtaatagtg tattgtatgc tgcactatct acaattgtta gtgttgcggt atgtagtatg
     2101 tatgcctatc cacttacaat tcctgaaatt aggggtaaaa aattctggaa tactcttatt
     2161 ttgattccaa tgttctttag tggaggaatg atcccgcttt atatacaagt tgcaaacctc
     2221 ggtttattga atactgcttg ggctcttgtt ttacctggtg ctgtttctac atattatata
     2281 attttgttta gaacacagtt taaatcaatc ccaatggagc ttcgtgagtc tgctagaatt
     2341 gatggggcaa gtgaatttag aattttttgg aatttagtat taccattatc aaaagttatt
     2401 ttcatgatta tagcacttta ttgcatagtt ggttcatgga atagttttac tcctccatta
     2461 ctttatttga atgacgacac taagatgcct ttatctattt atttgagaaa tttagtcgtt
     2521 acaaatacaa ctgatactgc tgaattatca ctttctgcag gtaatacata ttaccaacaa
     2581 attgctgctc aaggtggagg tattggtatg cgtattgcta ttaagatggg tactattgtt
     2641 gttagtgtta ttccaattat gatgatttat ccatttattc agaaacattt tgttaaggga
     2701 atcctgctag gttctgtgaa aggttaataa ggtaattatt ttgcaaaatt taaaaaaata
     2761 tcttttatta gatagcggtt tagtagttgg gtatacatta gaaaatgctc aattaaaatt
     2821 aaataagcta aaaaaagata ctgaaaacaa tccactattt ggtgaagagt attttgccga
     2881 aaatcctaaa ttatgggaag ttcgttttga taattcatat ccaaacgtta tctatgataa
     2941 agaattgaaa ttatatagat gttggtatca aacatttgtt agtgatgaag caagcgaaga
     3001 gactccttta gcagaacgtg gagagaagga atacattgtc aagtcatcta gaatgacagc
     3061 actttgctat gctgaaagta aagatggagt aaaatgggaa aaaccaaatc tcaatctagt
     3121 aaaatttaaa ggttcaaaag ataataatat tgttctgatg catgcacacg gtgcaggtat
     3181 catgctagat gaacaggagt tggatcctaa gaaacgatat aaattagttt caaaattaga
     3241 attttctccg aatcttcatt acatggttgt tgttttttca gaagacggta ttcattttga
     3301 cgaacctatt ccatggaaaa aatggaatcc tgcagcagat agtcataatt ttccatttag
     3361 agatccaaaa actggtaaat acgctgttat tacaagaacc tgggcaaata acgtacgtct
     3421 aagtgcaatt actttcagtg atgattttat taattggtca gaacctaaag aaatccttag
     3481 gggagatgga tttgaagatc aaatttattc tatgccagta atttcatacg cggatatcta
     3541 tttaggtttt gcttcaatgt accatgaagg cgacacttta aatgaaaact atgataaagt
     3601 agacttagaa ttgaaattct ctgctaattt ggaacaatgg gatagtgtat gtaagggcga
     3661 ctatctcatt gaaaggggag gtggagtata cccgcagaat gctgattttg atgctagttg
     3721 tattttcacg gctgctccaa taattgaaga tgataagatt tggttctact atatgggtgg
     3781 aaatggtaat cacactggct tccgagaaag ttcattagct agaggatata ttaagaaaga
     3841 ccaatttgct tattatacta ataagagaga aggaattcca gctaaaatat ctacagttag
     3901 cttccaaact tacgatgatg aaatttatat cttagctgat attcctgaaa atggttatat
     3961 taaagttgcg gtaggtacta aatacggtaa tgcttattca gatttcggtc aggaagatat
     4021 ctctcttgtt caagtttccg atggtaagta taagttggaa tttaataaaa aatctatctt
     4081 agatttaggt aattctccag ttgcgttcaa atttgaaatc gagaatgcaa aattgtatgg
     4141 tattgaaggt agtattcagc aacactatct caaatattaa ccagaataat tcgttaacaa
     4201 ataatctcta tattagtgga gatagatata taaaatggag gtcttattat ggtaaagctt
     4261 aataaggtat taaaagggag tgttgcattc ctgagtgcaa gtttattgct agcagcttgt
     4321 ggcaataacg ctaattcaaa gcccgctgaa aaaacagctg atggtaaaac gaaaatttcg
     4381 tttctatcta catggcaaat taatgatgtt cgtccgccta aagatgaaac acttgttggt
     4441 caatggttag aagaacagct aggtattgat attgatcttg taactattcc tggcgaggga
     4501 gtaagttcta aagtaaattc tcttattact agtaaccagt taccgactgt tttgttgaca
     4561 acaggagata agagtgatat cgctggtatc aacaaacttg gtaaacaggg tgcattctta
     4621 gatttgtcac aacacatgga caaactacca aactataaga agtaccttga aaaaaataat
     4681 gcattagctc aaatagaaga tgatgaaaaa catatttatg ctttcacaaa attctttact
     4741 gatgagaata tcatgtatac tacaccaatt ctgagaaaag acttacttgt tggttcagaa
     4801 tttgaagatc tttcaaaaat taaaactgtt gatgactata caaaagtttt gaaatatttg
     4861 actgaaaaac aaggttctcc agcatttatt caacgtaatg gctatgaagg cttcatgaag
     4921 cgagttacac cattgtggaa tttaagccat cgtacatact atgactatga atcagattca
     4981 tataaacatc cagttgaaca acctcaattg aaacaattcg ttgaatggtt gaaagaatta
     5041 cgtaaagata acgtacttca ccctgactgg gcagttatga aagatgaaac ttgggaaggt
     5101 ctattggctt ctaacaaggg ttcattcact gttgaccgta tgaatatcat tggagataat
     5161 aactttgata aatcatttga ttggcagcca cttgtttatc cagaaattaa tggaaaacgt
     5221 tatttgcaac cgaaaacacc gtacatttct gattcaggtt gggtaatcaa tgccaatgca
     5281 ccaaaagaag aaattgataa agcgttagaa ttctttaatt tcttatacgc tgatgaaaat
     5341 caagaaaaac ttgctattgg atttgaaggt aaaacattta cacgtgaaaa ccctaatact
     5401 caagcaggta ttaaatggtt gattcaaatt tacggtgaaa atgctggaaa tgataaagca
     5461 gaactgctgt ttaaacatgg taaccaagca tttactcgcg ttcaaactaa attagatatt
     5521 gaggcacagc caggaacatt ccctaaagtt atgtatgatc aagtagataa aattaagaaa
     5581 gatttaggtg gttttagacc agatactccg gttctttcct ttacaactga agaattagat
     5641 gttttaacta aatctgaagc aggattaaat actttctttg atgaaaatat tattaaattc
     5701 attgaaggac aaagagatat gagtgagtgg gattcattta tcaatgaagc taaatctaaa
     5761 gacttggata aagttacaaa agtatataat gatgcctaca aacgtgtcaa agatagaatg
     5821 aaatagacta aatggtagct ctctggtttt tcagagagct actactgcct gagggggagt
     5881 aagctttgta tgatgtagta attataggtg caggaagttc gggcgcattg gctgcaattg
     5941 cttcggggcg tctagggtta aaaacttgtc taatagagaa gggaagtaga attggtggag
     6001 ttcctattaa tacactaatg gggtcatttg ctaatctata ttttgatgat tctggtagag
     6061 taaatgcaag taaaattgtt ggggaattaa tagaacgtat tatcaaaaaa ggtggtactg
     6121 tctttaactc tatggaagaa ttgatgaatg taaatgattt ttatcagatt acaattccct
     6181 atcaaccaga agtttatgaa tcggttttaa ctgatatgtt gattgagtct aaagttgata
     6241 ttttactaaa tgttgaagtt aataatatta tttatgatag taaaaatatt aaaagtcttg
     6301 aaattttaag taatcaggaa gttaaattga ttgattctaa agtttttatt gatgctacag
     6361 ggaatgcaac aattgcttct gctagtggag ctgatgttta tgaaaaaaat tcttcttacg
     6421 gttgtttaat gagagttgga ggagttgata tctcaaagac tatagaacat attgaaaaaa
     6481 caaagcagtg gaactatata gaaggatata atcagtggtt agaagaagta ttagcaaatc
     6541 gaaagtacag agccactgga gcagttttaa aagatccagt ttcttatgat catgccccta
     6601 tgctttcaaa agacgaccat tttatgaatg atgaaaaatg gaattacatt aaagaaagat
     6661 gggaaaaatt tagatttatt tacactttag aagtatcact atttaggaat gaattaaaaa
     6721 aagctgtaga atctggggat tttaattttg aaatttatca tgatgataaa tctggagtca
     6781 ctatgaatgg cgacggtata agctatggtt catggggtca aaatgtagca ttaattaatg
     6841 ttgcaaaagc atttggattc aatcccaaat atatggaaga tgaatcacat gctaatattc
     6901 tttctagaaa atataattta acatttttat cttttcttaa aaaatatgta ccaggttttg
     6961 aaaattcttt tatccttgat cagggaacta gagctactaa cagatcatgt aggcacattg
     7021 ataattcatc tatgatggac gaaattcttg attttccaat gtatacattt aagactatgt
     7081 atagctatca aactcctaaa gaaataacgt acaaatcaat tgtaggtgga aaattgaata
     7141 atttgtttgt aataggtaaa ggtgcttacc aatccgaaag ttttagatca caaattagtt
     7201 gtatgttaat ggggatatca tcagcagcag cggcaaaaac tattattgaa gataaaagca
     7261 atactctaaa cattaataga gatttgttta aatcgaactt agatatatta tttacatggg
     7321 agttttctaa ttggattact gattaacaaa taataaggga atattaaatg aatgatacta
     7381 ataaaaatca atactttgtt tatgaatttg atactagctt tgtagaaatg tcttattctc
     7441 agttttttga aggatattgt attctttttt caaaagaacc ctatccttct ttatctgata
     7501 tgccaatgga atttagaaaa gaatttttaa tggaaatgag tatattaggt gatgcgatgc
     7561 ttgaagtgtt aaatgcctat agaattaatt atgatatctt aggaaattca acacctgtac
     7621 ttcatgctca tttatttccg agatacatgt gggaagagga agagagaaga aaaactgtag
     7681 ttcatagata tgattcttca gtttttagca atcctgagtt tgaatttact atagaaaagc
     7741 atggaccaat tattaaaaaa ctgaggtctg ctattgatag acattataga gagtatcaac
     7801 aattattaga tgattcaaac tgaactccac attttcgatt agatttaata ccttgtatat
     7861 tacaactagg ttgtggatga tataattttt tgaaaaatat attttaggaa tgacttataa
     7921 agcaattata tcagaggcta ttaaatcaat ggccaaagct tatgctcctt attctgattt
     7981 taaggttgga gctgcgttag taactgaaga taaaaaattt tttctggatg taatatagaa
     8041 aatgctgctt tttctcctac ttgctgtgca gaacgagtat ctttttataa agctgtattt
     8101 aaaggagaac gttcgttttc aagaacatgt attgtaggtg gaaaaaatgg tatcttatca
     8161 tcttttatag caccttgcgg agtgtgtcgc caggtaatga tagaatttgt aatcctgatt
     8221 catttgaagt tatattggct agtgatgttg atacttataa agtatataaa cttaaagaac
     8281 tactaccagt tagttttaat caagaaaatt tataacagga gggaactatg gaattagttg
     8341 aatttaaaaa tggacatgtt aatacggttc atgagcatat cgcaataacg aaagtaaaag
     8401 agtttctaaa taatcatgat atattaaaag ataaaggtgg catacataaa atttctactg
     8461 atttctttta caatataatt gatatggaaa ctacaactga agagaataga ccgtgggaaa
     8521 gtcataagga atattatgat gtccattatc tgttaaatgg agaagaaaca attttatata
     8581 acttcttgtc tcaaatggaa ttgtctgaat ataaagtaga tgatgattgg caacaaatga
     8641 atgggacagc tttatttagt attaaattaa agaaagatat gttacttttg ttggaaccaa
     8701 acgatgcaca tagaacaggc ctattggttg aagaaccact taatattaag aaagttgttt
     8761 ttaaggtaaa aatttaactg aaagtacttt cggggattaa gaattttaaa atatttaaga
     8821 aattgtattc taaagttcta aaattataga tatttaattt aaagcttgtt aaaatagaat
     8881 agtaacactc atctgctttc aaaaagcatt ctattccatc tccgattaac gatggacttt
     8941 atcacctcct tctccagtcc ttgtatgaca tcttggagtt gattcatgac atcttccaaa
     9001 gttcgaaagg ctttattctt aaatccacgt ttatgaatct ctttccacac ttgttcgatg
     9061 ggttcatctc tggtgtgtat ggaggaataa atgcaaaacc aatattagtc ggagtcttta
     9121 aggtacttga tttatgccat atagcattgt ccataacgag taaaagataa tcatctggat
     9181 aagcttgtga aagctcttct aaaaaggcgt tcatccactc agtattacat ccaccagcta
     9241 ttaagaaaaa tgattcgcct gtatgggcat caacagctcc ataacaatag tgaaattctc
     9301 gtatatagtg actatggaca tgtggaccta ctcctattgg agaccaacaa gatcccagtt
     9361 tactgattct accgaagcca gcctcatctt ggtacatcaa gcgaac
//
LOCUS       DAVBCN010000072         8978 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVBCN010000072 DAVBCN010000000
VERSION     DAVBCN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8978
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.072"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(1..781)
                     /locus_tag="VQ497_001690"
                     /pseudo
     CDS             complement(1..781)
                     /locus_tag="VQ497_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(992..1351)
                     /locus_tag="VQ497_001691"
     CDS             complement(992..1351)
                     /locus_tag="VQ497_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HET2517974.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            1443..2399
                     /locus_tag="VQ497_001692"
                     /pseudo
     CDS             1443..2399
                     /locus_tag="VQ497_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            2383..2940
                     /locus_tag="VQ497_001693"
     CDS             2383..2940
                     /locus_tag="VQ497_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2517975.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQADLTNAQLILQGHETLDQFVIKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            2933..3187
                     /locus_tag="VQ497_001694"
     CDS             2933..3187
                     /locus_tag="VQ497_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517976.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERSVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            3199..5253
                     /locus_tag="VQ497_001695"
     CDS             3199..5253
                     /locus_tag="VQ497_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET2517977.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            5432..6292
                     /locus_tag="VQ497_001696"
     CDS             5432..6292
                     /locus_tag="VQ497_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET2517978.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLCFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            6503..7213
                     /gene="ccdA2"
                     /locus_tag="VQ497_001697"
     CDS             6503..7213
                     /gene="ccdA2"
                     /locus_tag="VQ497_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET2517979.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            7233..7799
                     /locus_tag="VQ497_001698"
     CDS             7233..7799
                     /locus_tag="VQ497_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HET2517980.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            7810..8922
                     /gene="msrB"
                     /locus_tag="VQ497_001699"
     CDS             7810..8922
                     /gene="msrB"
                     /locus_tag="VQ497_001699"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET2517981.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
BASE COUNT         2551 a         1674 c         1973 g         2780 t
ORIGIN      
        1 cctctcagat tacatctctt gtaacccaaa caagccttta tctgcccaaa gacaggttcc
       61 acatcaatct tgcgttgagc gaaaatctgt ctaccttggg gagataaaag cgcctgacat
      121 tctttagctt tcaagttttg atagcgttcg ttcatataca gtcccttttg agggactgat
      181 tcaggttcat cagcgtagta aaccttgatt tcctgttgaa agtctgtctg tgttttctgg
      241 tacttgatat ggtgaaaacg atagcaccag ccatcaggat gtgtgtagct atcctccttg
      301 tcattatagt gccaattcgc taagttttta gctgactgtt tataccctct cttttgttcc
      361 ttatcaaaca tggcatattt aatcagaagg ggagtgattt cttcttgaaa atagacctgt
      421 atctgttctt gaagtttggc ggagaatttc tctgtcgttt tcttccacac aaaactatac
      481 ttgttggcat tggcttcaat cttagttccg tcaataaaca aacaatctaa ggtcactaac
      541 tcttccattt ttcaattttt cgaccagaga aaatcccttg tgaataggca aatagaagag
      601 tagatacaag cattttaggg tgataagacg ggcgatcaaa ggcatgatag aaggcgtgga
      661 agtgacaatc ctccaaggta ttcaccactt tttcaatagt aaaaacgaga tgatcttgtg
      721 gcaagaagga actgatttct agtggtaaag ttgtttgatt tgtgttatag tgaatatgca
      781 tgggatagcc tttctaaatg gtttattaag caattctatt ttaccaagac tatcgcaacc
      841 atgcaaaaaa gcaacggcaa atccgttgcc ttttttggag ataagagact attgtcccag
      901 actcttttct ttgtataaaa ttgtatagtg ttattgtcta tactattttg tagagaaagc
      961 aaaaatccct agaaaatgct tagggattcg actatttcat taagaacaat tcaacaatca
     1021 tttctatata catgataagg gttaataaga atcctgcacg ccagaagaat ttgataaatt
     1081 taggatagta aaagctgcgt tttttcaaaa ggaagaaaaa gacgagaata atggctagga
     1141 tcgagagggc tagccccagt ctagggagga aattatgggt aaaggtttta gctgtaatca
     1201 agtaatactc aaataccaag actggaaaag ccaaatccgc aaagtttcgt cctagttttt
     1261 ttaatctaaa aagtttggtt aggataatgc agacgactaa ggtcagtatc aataataaaa
     1321 tagatgctaa tttcattaaa atcataccca tattgtatca taaaaaaagg ctaatggaaa
     1381 cgagaaacag aggaatttat aggaaagtca ggcttttgag attgtgggtg atttttgtta
     1441 taatgaaagt tataaaatct tataatacct tgaatgatta ttatcgaaaa ctctttggag
     1501 aaaagacttt taaagtccct attgatgcgg gatttgactg tcccaatcgt gatggaactg
     1561 tggctcatgg aggctgtact ttttgtacgg tttccggttc tggagatgcc attgtggcac
     1621 cagatgcgcc tatccgtgag caattttata aggaaattga ctttattcat cgtaagtggc
     1681 cagatgttca gaagtatctg gtttattttc aaaattttac caacacccat gaaaaggtgg
     1741 aagttattcg agagcgttat gaacaggcta tcaatgagcc aggtgtggta ggaatcaata
     1801 ttggaacgcg cccagactgt ttaccagacg aaaccatcga atatttggct gagttatcgg
     1861 agtgcatgca tgtgacggtt gaattgggct tgcaaactac ttatgaagca acctctgacc
     1921 tgattaaccg tgcgcattct tatgaattgt aggtggaaac ggtcaagcgt ttgagaaagt
     1981 atccaaaaat tgagattgtt tcccatttga tcaatggttt gcctggtgaa acccatgaga
     2041 tgatggttga aaatgtccgc cgttgtgtca cggataacga tattcaaggg attaaactgc
     2101 atttgctcca tcttatgaca aatactcgta tgcaacgaga ttaccatgag gggcgtttac
     2161 aattgatgag tcaggacgaa tatgtcaggg tcatctgtga ccaactggaa attattccca
     2221 aacatatcgt catccatcga attacaggag atgcgcctag ggatatgctg cttgggccta
     2281 tgtggagcct caataaatgg gaagctctca acagcattga gatggagatg cgacgtcgtg
     2341 gaagtgttca aggatgcaag gctgtaaaac aggagtttga aaatgaaaag accacttgag
     2401 atggcacatg attttttggc tgaggtcgtg acaaaagagg atgtcgtagt ggatgcgact
     2461 atgggaaatg gtcatgacac gcttttttta gccaagctag ccaagcaagt ctatgccttt
     2521 gatattcaga agcaagcctt ggaaaagacc caagagcgtt tgcatcaggc tgacttgaca
     2581 aatgcccagt taatcttgca aggccatgag acactggacc agtttgtgat aaaagctaag
     2641 gcagggattt ttaatctggg ctatttgccg tcagctgata agtctgtcat cacccgaccg
     2701 cagacaacga ttgaggcatt agaaaagcta tgtggcttac ttgtcaaagg tggacgaatt
     2761 gctattatga tttactatgg tcatgaagga ggcgatctcg agagagatgc tgtcttggat
     2821 tttgtgatcc agttgaacca acaagagtac acagctgcca tttaccgaac tttaaaccaa
     2881 gtcaacaacc cgccgttttt agtgatgatt gaaaaattag agagatacag acatggataa
     2941 acaataccta cacgaaaagc tggatgccat gcgccagaat tttgttgaat caacccacca
     3001 cgaacgatcg gtgggcgtgc tagaccaagc gcatatgagc aaaaaaatgc ttaaaatcaa
     3061 gaaaaaatta gttgctcttg aaatggaacg gtgccagaga aaaattgagc acaaagactg
     3121 ttccaagatt gaccaaaaaa tcaaagagca gaaggagata tttgaatcct gttgtaaaaa
     3181 agattaagga ggacgtgcgt ggaattactg atttacctca tcctattttt actggttttg
     3241 attgtctcaa gtacaaccaa taagctcctg ccctttttgc ctctcccttt ggtgcagatt
     3301 cttttgggaa ttgtgattgg tctcttttta cccaatactg actttcacct taatacggag
     3361 ttgtttttgg cactggttat cggacccttg cttttccgag aggctgaaga agcagatgtt
     3421 acggctattt taaaacactg gcgaatcatt gtttatctca tatttccagt gatttttatc
     3481 tcgaccttga gtttgggtgg cttggcccat cttctttggt tcagccttcc cttggcagct
     3541 tgcttggctg ttggggcagc ccttggtcct acggacttgg tggcctttgc ctctctttcg
     3601 gagcgtttta gctttcctaa gcgcgtgtcc aatattctta agggcgaagg actcttgaat
     3661 gatgcttctg gtttggtggc ttttcaggta gctttgacag cttggacaac tggagctttt
     3721 tctctggggc aagctagcag ttcgctcatc ttttcaatcc taggcggttt tttaattgga
     3781 tttttaacgg ccatgaccaa ccgcttcctc cataccttct tgctaagtgt gcgcgcaacg
     3841 gatattgcca gtgaactttt attagaattg agtttgcctc tagtgacctt ctttctggca
     3901 gaagaagttc atgtttcagg tattattgcc gtcgtagttg ctggaatttt aaaggcaagt
     3961 cgcttcaaga aaatcacgct cctcgaagcc caagtggata cggtgaccga gacggtctgg
     4021 catacagtga cctttatgct caacggttct gtctttgtga ttttagggat ggagttggaa
     4081 atgatagcag aacctatctt gaccaatcca atctataatc ctctactttt attgctatct
     4141 ctcatcgccc ttacctttgt cctctttgtc attcgtttta ttatgatcta tggctattat
     4201 gcctatagaa cccgacgcct caagaaaaag ctaaataagt atatgaagga catgtttctc
     4261 ttgacctttt caggtgttaa gggaacggtg tcgattgcta cgattctctt gataccaagt
     4321 aatctagaac aggagtatcc tcttttgctt ttccttgttg caggtgtgac gcttgtcagc
     4381 tttttaacag gtctcttggt cttgcctcat ctttctgatg aagaggaaga aagcaaggat
     4441 tatctcatgc atatcgccat tttgaatgaa gtaacgctag agttggaaaa agagttggaa
     4501 gacaccagaa ataaacttcc cctctatgcg gctattgaca attatcatgg acgtattgaa
     4561 aatctcattt taagccaaga aaaccaggat gatcaagaag attgggctgc tttgaaactc
     4621 ttgattctta gtattgaaag tgatggtttg gaacaggcct atgaagaggg gaacattagc
     4681 aatcgtgctt accgagttta ccaacgttat ctgaaaaata tagaacaagg aatcaatcgt
     4741 aaacttgcct caagactgac ctattatttt cttgtttcct tgaggatttt acgttttctc
     4801 cttcatgaag tttttactct tggaaagacc ttccgtagct ggaaggacaa ggagcaaagc
     4861 cgtctccgtg ctcttgatta tgaccaaatt gcagagctct atcttgccaa tacagagatg
     4921 attattgaaa gtttggaaaa cctgaaggga gtctacagac gctctttgat tagttttatg
     4981 caggagtctc gtcttcgaga aacagctatt atcagcagtg gtgcctttgt cgaacgggtt
     5041 atcaatcgtg tcaaacccaa caatatcgat gaaatgctga gaggctatta tctggagcgc
     5101 aagttgattt tcgaatacga agaaaaacga ttgattacga ctaagtatgc caagaaatta
     5161 cgacaaaatg taaataactt agagaactat tccttgaagg aagctgccaa taccctgccg
     5221 tatgatatgg tggaattggt aagaagaaat tagttaatac tcttcgaaaa tctcttcaaa
     5281 ccacgtcagc gtcgccttgg attatatatg tgactgactt cgtcagtttc atctacaacc
     5341 tcaaagcagg gctttgagca acctgcggct agcttcctag tttgctcttt gattttcatt
     5401 gagtataaga ttataaatga aggagtatga catgaaaaag tggtggaaag agctgataga
     5461 caagccttta ttaaaagctt ttttgcatta ttatcaagca tcagatagtg agttgaccag
     5521 tgtcgcagta gcctactatt ggttgatttc gattttcccc ctgcttttgg tggtggtcaa
     5581 tatcctcccc tattttcaga ttcctgtggg agaatttctg tgctttatga aagacgttct
     5641 gcccccaagc ctctatgaag gtgtggaaaa aatagccaga gaagttttga cgcaaccttc
     5701 aacaggttta ttgagttttt ctgttttatc ggcgttatgg agtttttcaa aatctatgaa
     5761 tttcttgcaa aaagctttta acaaggctta tggcgtcgaa aagagtcgtg gacttatttc
     5821 ccatcagatg ctaagtctcc tagtcagttt tggcttgcag ctcctctttg cgtttgcctt
     5881 gtttttgatc ttatttggtc agatgatttt ggctttactt gctcattatt ggacaaaaga
     5941 tggcattatc tatcaggctt tacaagggtt ggcaggtcct ctgatttacg ccctcctctt
     6001 tgctatcttg gtcatgcttt attattttct tcccaatcta tctaacagga aaattagcta
     6061 taccctacca ggcagtgcct ttgttctctt agtgatttta ggtttactaa ctctgttctc
     6121 tagttatctc aattactatg tccatcattt ggtggatgtc cgaattttag gttccgttct
     6181 tcttgttgtt atgatgtttt ggtttatctt gattgctaaa attgttatcc taggcgcggt
     6241 tatcaatgcc agtatgcaga gtttgaaaga tccggtcttt aaaaaagact aactatttat
     6301 ccattacaaa agcgcatagt atcaaaactt tatagaacct tgatactatg cgcttttgtg
     6361 ctttgacagt tttttgtata gtttttgtcc aagatttcta aatcatctga ttaaattcct
     6421 attttcccct atcattgtcc ctgttttttc gaaaatggaa ggagtataat aaatctatca
     6481 ataaaatgat aggaggaact ttatgggtca tattttcttt tttctaagtg tctttttggc
     6541 agggattcta tccttctttt ctccttgtat cttacctttg ttaccggtct atacaggagt
     6601 gttactagat gataaggatg gtgctcaggc ttctagcggc aaattttcaa tctcagttac
     6661 tagtttatta cgaactctgg cctttatagc aggaatttcc tttatattta ttttgttggg
     6721 ctatggagct ggttttttag gcgatttgct ttatgcttct tggttccaat atcttactgg
     6781 ggcaattatt atccttcttg gtttgcacca aatggagatt ctacacttta aggggcttta
     6841 taaggaaaag aggctacaac tgcaaggaca ggggcaaaat ggtaagggct atagtcaggc
     6901 atttttattg ggcttgacct ttagttttgc ttggacgcct tgcgtggggc cggttctggg
     6961 gtctgttttg gccttggcgg cttcaggtgg ttcaggagct tggcagggag ctggtctcat
     7021 gttggtgtat acgctgggct tggcgctacc attcttgctt ctagctctga cctctagtta
     7081 tgttttgaaa catttccgaa aacttcatcc ctatctcgga atcctcaaaa aagtgggtgg
     7141 ttttctcatt attgtgatgg gactcttggt tctgtttgga aatgcttcaa ttttaagtca
     7201 attatttgaa taaaatggaa aggaatatca atatgaaaaa atggcaaaca tgtgttcttg
     7261 gagcaggttc gctcctttgt ttgacggctt gttcaggcaa gtccgtgact agtgaacacc
     7321 aaacgaaaga tgaaatgaag acggagcaga cagctagtaa aacaagcgca gctaaaggga
     7381 aagaggtggc tgattttgaa ttgatgggag tagatggcaa gacctaccgt ttatctgatt
     7441 acaagggcaa gaaagtctat ctcaaattct gggcttcttg gtgttccatc tgtctggcta
     7501 gtcttccaga tacggatgag attgctaaag aagctggtga tgactatgtg gtcttgacag
     7561 tagtgtcacc aggacataag ggagagcaat ctgaagcgga ctttaagaat tggtataagg
     7621 gattggatta taaaaatctc ccagtcctag ttgacccatc aggcaaactt ttggaaactt
     7681 atggtgtccg ttcttaccca acccaagcct ttatagacaa agaaggcaag ctggtcaaaa
     7741 cacatccagg attcatggaa aaagatgcaa ttttgcaaac tttgaaggaa ttagcctagg
     7801 aggtgtctta tgaatgataa gttaaaaatc ttcttgttgc taggagtatt ttttctagcc
     7861 ataaccggtt tctatgttct attgatacga aatgcagggc agacagatgc ctcgcaaatt
     7921 gaaaaggcgg cagttagcca aggaggaaaa gcagtgaaaa aaacagaaat tagtaaagac
     7981 gcagacttgc acgaaattta tctagctgga ggttgtttct ggggagtgga ggaatatttc
     8041 tcacgcgttc ccggggtgac ggatgccgtt tcaggctatg caaatggtag aggagaaaca
     8101 accaagtacg aattgattaa ccaaacaggt catgcagaaa ccgtccatgt cacctatgat
     8161 gccaagcaaa tttctctcaa ggaaatcctg cttcactatt tccgcattat caatccaacc
     8221 agcaaaaata aacaaggaaa tgatgtgggg acccagtacc gtactggtgt ttattacaca
     8281 gatgacaagg atttggaagt gattaaccaa gtctttgatg aggtggctaa gaaatacgat
     8341 caacctctag cagttgaaaa ggaaaacttg aagaattttg tggtggctga ggattaccat
     8401 caagactatc tcaagaaaaa tccaaatggc tactgccata tcaatgttaa tcaggcggcc
     8461 tatcctgtca ttgatgccag caaatatcca aaaccaagtg atgaggaatt gaaaaagacc
     8521 ctgtcacctg aggagtatgc agttacccag gaaaatcaaa cagaacgagc tttctcaaac
     8581 cgttactggg ataaatttga atccggtatc tatgtggata tagcaactgg ggaacctctc
     8641 ttttcatcaa aagacaaatt tgagtctggt tgtggctggc ctagttttac ccaacccatc
     8701 agtccagatg ttgtcaccta caaggaagat aagtcctaca atatgacgcg tatggaagtg
     8761 cggagccgag taggagattc tcaccttggg catgtcttta cggatggtcc acaggacaag
     8821 ggcggcttac gttactgtat caatagcctc tctatccgct ttattcccaa agaccaaatg
     8881 gaagaaaaag gctacgctta tttactagat tatgttgatt aatactcttc gaaaatctct
     8941 tcaaaccacg tcagcgtcgc cttaccgtat gtagggtt
//
LOCUS       DAVBCN010000073         8957 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVBCN010000073 DAVBCN010000000
VERSION     DAVBCN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8957
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.073"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(169..972)
                     /locus_tag="VQ497_001700"
     CDS             complement(169..972)
                     /locus_tag="VQ497_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HET2517982.1"
                     /translation="MQSTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRSEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(1249..2004)
                     /locus_tag="VQ497_001701"
     CDS             complement(1249..2004)
                     /locus_tag="VQ497_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HET2517983.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(2074..4725)
                     /locus_tag="VQ497_001702"
     CDS             complement(2074..4725)
                     /locus_tag="VQ497_001702"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET2517984.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYSTTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPKFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTDDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNEAGEIYVGEEAPAGDGWKQDEDVLDTWFSS
                     ALWPFSTMGWPNTETTDFKRYYPNSTLVTGYDIIPFWVSRMIFQGIEFTGKSPFKNAL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIDKYGTDSLRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAEYPTVNPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLVDLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            complement(4718..5296)
                     /locus_tag="VQ497_001703"
     CDS             complement(4718..5296)
                     /locus_tag="VQ497_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET2517985.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYQLMYLKQIQNLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKHKPLKRHIEVSEREILETAETLQLNSQYQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            complement(5296..5766)
                     /locus_tag="VQ497_001704"
     CDS             complement(5296..5766)
                     /locus_tag="VQ497_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2517986.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGYG
                     IVVKGTDKIVGSVDFNHRHEDDVLEIGYTLHPDYWGRGYVPEAARALIDLAFKDLGLH
                     KIELTCFGYNLQSQRVAEKLGFTLEARIRDRKDAQGNCCDDLRYALLKSEWEVI"
     gene            complement(5753..6331)
                     /locus_tag="VQ497_001705"
     CDS             complement(5753..6331)
                     /locus_tag="VQ497_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET2517987.1"
                     /translation="MKQSFKTNKLYYGFPVFILGYQDQNFGYNITTCSSSYSLGDWVV
                     IGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEKLERLGVHYEISERTQ
                     TPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDEKGHFKNQLFAPTYFM
                     GDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            complement(6614..7009)
                     /locus_tag="VQ497_001706"
     CDS             complement(6614..7009)
                     /locus_tag="VQ497_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HET2517988.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(7105..7359)
                     /locus_tag="VQ497_001707"
     CDS             complement(7105..7359)
                     /locus_tag="VQ497_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HET2517989.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            complement(7368..8702)
                     /locus_tag="VQ497_001708"
     CDS             complement(7368..8702)
                     /locus_tag="VQ497_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HET2517990.1"
                     /translation="MTDEWIHILRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVLDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYELMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTVKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKRLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(8702..8935)
                     /locus_tag="VQ497_001709"
     CDS             complement(8702..8935)
                     /locus_tag="VQ497_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HET2517991.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLTGTPMDKIVRTLEANGYEVIGLD"
BASE COUNT         2527 a         2012 c         1698 g         2720 t
ORIGIN      
        1 taaaactaaa aggacttagc cttgtgcaat acagaactaa atccttcgga taaattattt
       61 gtctaacttt ttggggtcag tacacctaaa actttgatga tatacgtttt cttgtgagaa
      121 tatttacttc atttttgcct aaaattcaat gtttactcag tatttggatt atgaaaaatc
      181 gaggtctaaa tctagataca ttttttctga agacaaatca ttttgaccac cgagcaagag
      241 attttcaaaa aaagctgtta aaaactcaga acgtcgctgt aaaatctttg cattatctaa
      301 taccaaggca tcacgaaaat atttggaatg ttgctgaaat ggtgtattat caatatcaaa
      361 accaaactca cgaagatact gaatcaaaaa gaccgttact gtccgagtgt ttccttcgcg
      421 aaatggatga atctgccaga ttcctgaaat aaaatgctgg atttgtttaa ccacatccgc
      481 ctgagttagt gtcgcatatg caacttgttt ttcctgatta aaatcataat ctaaggtcat
      541 ttgaatcatg gagtaatcag agtacacaac actttcacca ttcaaaacag gttcattctt
      601 tgtgatattg gtctgacgaa attgccccac cggaatagag ggttcaaata tatcttgaaa
      661 caactcctta tgaatagcaa gtaaggtcgc aggactaaag ctaaagcctc ttcgagacaa
      721 tagttctaca atacgtagag aaaccaagtc tgcctcttcc gtacttgcat caatagtatg
      781 gtgataagcc gttgcatcct cataaacctg ctcataagtc agttctcccc gggactgttt
      841 ctcagccaaa gattccatat acgctgatgg cactagattg tcaactttct gcagaccaaa
      901 acctatccgc cataaatcac gcttcgcttc ataagacaag tttggattgt caatgttgta
      961 agttgattgc atagaaatat cctttcaatt gtttttcttc attaccagta aatcacgaaa
     1021 ttggttggaa actggcttaa actataaaaa gtcctcacaa taggttctat gatatatata
     1081 tatacttcat gtaaataata aaagagttat aatcagcact actgataaat tcaataccga
     1141 tttcgccatc ttaaaactta gtcaaacgaa taccgttgat ttccagttca ttaagtcgtt
     1201 gacctgtcaa gacttctcgt tccttcaact ggaaaagtta agggggaact aatacaaatc
     1261 ctctacgctt ctatattcaa tgatttcatt tttgataata ttctttttaa actcaaaatg
     1321 ttttaaagga ttatcgatta acactaactt tggaatatca tctagatttg tgaccaagga
     1381 taaaataaaa tccgattgaa actccttgac tttttcaaat tcttttgcag ttaaacgcac
     1441 acgcccctta gattttcgga taccttttac ttcggcaagg taagaatact cttggacatc
     1501 tacctgaaag tcatatccat caccataaag acgggcatcg gttaactgtc caccttggaa
     1561 tttttcttct ttatcaaaat gaaggataaa ataattctca gcctccattc ccgtttcctg
     1621 tagacgtttg aatttagttc gaactattgg tttttcaacg atggttatgc ctgttttctt
     1681 cccttcactg gcaagtaaca ttttaactat ttcagcataa ctatgaacat cttcatttcc
     1741 aaacatcata tcaattaaat ctttacgaaa acgataaact tcagctttct gccaccaacc
     1801 ttttcgatta ttgtcaaaat aaggatcaaa taaatccatt ctatttttta caactccagt
     1861 tgtttcagca attcccagag aaaccacata gcgataaaac tccgacttgc tagagcattg
     1921 aaattctttt ataaaaagct tgtcaaactt ggcaagaccg tagccaatta aatttaacaa
     1981 ttcataatga gggtgtttgg acatcacttt ctccatcaat agatattgat ttcattatac
     2041 caaaaaacac ggctcatcac cgtgtttctg tgtttatttc accaacttct tcatctcatc
     2101 aatacgtgct acggtcgcat catacttagc ttggtaatct gcttgcttgt cgcgttcttt
     2161 ttggacaact tctggtttag cgttggcgac gaagcgttcg ttgctgagtt ttttgccaac
     2221 catgtcgagt tctttctgcc atttggcaag ttctttttcc agacgtgcca attcttcttc
     2281 gacattcaag aggtctacca gtggcaagaa gatttcggct cctgtgatga cgcttgacat
     2341 agccagttca ggtgcaggga tggttgatgc gatttccaag tgttcaggat ttgtgaaacg
     2401 tttgatgtag ttgacattgc tgttaaagaa ggcttccaag tcgctatcgc ttgtcttaac
     2461 aaggatagtg ataggcttgc ttggagcaac gtttacttcc gcacgcgcat tacgaacggc
     2521 acggatcaag tctttaaggc tttccacacc agtgtgagca gcaaggtctt caaaggctgg
     2581 gttgacagtt gggtattctg ctgtaacgat agatccttct gagatttgtc caaagatttc
     2641 ctctgtcaca aatggcatga ttgggtgaag gagacgaagg atcttgtcca aagtgtatag
     2701 tagaacagaa cgtgtgatga ctttctcttc ttcgttatcg ctataaagga cttccttggt
     2761 caactcaaca taccagttgg caaattcttc ccagatgaag ttgtaaagga tatgaccagc
     2821 cacaccaaac tcgaacttat caaagttttc agtaactttt gcaatggttt cgttgagatt
     2881 gtggagaatc cagcggtccg tcacattacc agcctcacct gttgcaactt ttgtgacatt
     2941 gtcatgcgcc acatccagcg tcaaaccttc attgttcatg aggatatagc gagaaatgtt
     3001 ccaaattttg ttaataaagt tccatgaagc atccattttc tcgtaagaga aacgaacgtc
     3061 ttgaccagga gctgagccgt ttgacaagaa ccaacgaaga ctatctgtac catatttatc
     3121 aataacatcc attgggtcaa tgccatttcc aagagattta gacatcttac gtccttcttc
     3181 atcacgaatt agaccatgga taagagcatt tttaaatggg ctctttcctg taaattcaat
     3241 tccttggaaa atcatgcgtg atacccagaa aggaataata tcatagcctg ttacgagtgt
     3301 tgaatttgga tagtagcgtt taaagtcagt agtttcggta ttaggccagc ccatggttga
     3361 gaagggccag agggcagagc tgaaccaagt gtcaaggaca tcttcgtcct gtttccagcc
     3421 atcgcccgct ggggcttcct cgccgacata gatttcgcct gcttcattat accaagcagg
     3481 gatttggtga ccccaccaga gctgacggga aatcacccag tcgtggacat tttccatcca
     3541 ttgcaggaag gtgtcgttga agcgcggtgg gtaaaattcc accttgtcat ctgtgtcttg
     3601 gttggcaatg gcgttcttgg ccaattggtc catcttgacg aaccattgag tagacaagcg
     3661 tggctcaact acgacacctg tccgttctga gtgaccgact gaatggacac gtttttcgat
     3721 tttgacaagg gcaccgattt cttccaactt agcaacgact gccttacgag cttcaaaacg
     3781 atccatgcct gaaaattcaa aggcaagctc gttcatagtt ccgtcgtcgt tcatgacgtt
     3841 gacttgtggc aagttatgac gttggccaac caagaagtca tttggatcgt gggcaggtgt
     3901 gattttcacg acaccagtac caaacttagg atctgcgtgc tcatcaccaa cgattgggat
     3961 taatttatta gcaattggaa ggatgacatt tttaccaatc aagtccttgt agcgcgggtc
     4021 ttctgggttg accgcaaccg caacgtcccc aaacatggtc tcaggacgag ttgtagcaac
     4081 ttcaagggcg cgtgaaccat cttccagcat gtaattcatg tgatagaagg caccttcgac
     4141 gtccttgtga ataacctcga tatcagaaag ggctgtgcga gctgctgggt cccagttgat
     4201 gataaactca ccacggtaga tccagccttt cttgtaaagg tccacaaaga ccttacgaac
     4261 agcttttgac aaaccttcgt caagagtgaa acgctcacga gagtagtcta cagagagccc
     4321 catcttgccc cattgttcct tgatagtagt ggaatattcg tctttccatt cccagacttt
     4381 cgtcaagaaa gactcacgac caaggtcata gcgggaaatg ccctcaccac gcaagcgctc
     4441 ctctacctta gcctgagtgg caatccctgc gtggtccatc ccaggaagcc aaagggtatc
     4501 aaaaccttgc atgcgttttt gacggatgat aatatcttgc aaagttgtat cccaagcgtg
     4561 accaaggtga agtttacctg taacgtttgg tggtggaata acgattgaat aaggcttagc
     4621 cttttgatcg cctgaaggct tgaaaacatc agcatcaagc catttttggt aacgaccagc
     4681 ctcaacctcg gctggattgt atttagatga aagttcttta gacatgtgtg tgtcctttct
     4741 ctattttgtt tattttattt tgaatttgct tagcagcttc ttctgcagac aaattcgtat
     4801 tatttatttt aaagtagtgg tgcaactcat tcggttgata ttgggaattt aattgaagtg
     4861 tttcagcggt ctctaaaatt tctctttcag atacctcaat atgtcgtttt aagggtttgt
     4921 gctttaatcg attctccgtt cgatttcgac gtatgcactc ttcaagactt gtttccaact
     4981 caacaaaaag aatctcttga tgataatcat ccaacaaatt ctgaatttgc tttaaataca
     5041 tcagttgata ctgatttgaa aaatcaatta catctgttaa aattactgat cgctgatttc
     5101 ttgcacttgc tccaaggaat gaaaaagtga ttccacgaac aaattcccac atttcctctg
     5161 tatagtcctg atagatttct agtgcaaaat caatggcttg atggttataa aatagggtag
     5221 catccgtcag tcgagataat tcttgaccaa tggtcatttt tcctgatgct ggagcaccaa
     5281 tgatgaaaag atgcatcaaa tcacctccca ctcactcttc agcaaggcat atctcaaatc
     5341 atcacagcag ttgccttgag catctttacg gtctcttatg cgagcttcga gggtaaagcc
     5401 aagcttttcc gcgactcgtt gactttgaag gttatatcca aagcaagtta gttcaatctt
     5461 gtgaagaccc aaatctttaa aggctaagtc aatcaaggca cgcgcagctt ctggcacata
     5521 acctcgcccc caatagtctg ggtgtaaggt atagccgatt tccagcacat catcttcatg
     5581 gcgatggttg aaatcgacag agccaacgat cttatccgtt cctttgacga caatcccgta
     5641 gcctgctgga taggcgacct ctggcaaact agcatagtca aagatatcct cagcatccgc
     5701 cacagtacgg actcgtaaaa cgagacgttc tgtttcgatt ttatctggca gctcagttct
     5761 tgccatcctt cttcctcgct tttttgatga aactgccctt catatctaca cgcttatcca
     5821 gatagcgata aacgcgctga tatccatctc ccatgaaata ggttggggca aacagttgat
     5881 ttttaaaatg tcccttttca tccaggagtt ctggggcaac aagtcgctca agaatcttgg
     5941 caaagatgtg gcaaataccg tcttcctcaa caatcctatc tacccgacaa tctaaaacaa
     6001 gtggacaggc gtctaaaata ggagtctgag ttcgttcaga aatttcataa tgcactccca
     6061 aacgttccaa tttctcctga tgactgataa aaccagcctg ctccatcgca agcatagaag
     6121 tttcatcaga aatattcaca gtaaattttt gatactgttt gatctgctct gcggcattct
     6181 ctctcgcaac gactccaatc acaacccaat ctcctagact atacgaggaa ctacaggtcg
     6241 tgatgttata gccaaaattc tgatcttgat atcctaaaat aaaaacagga aaaccataat
     6301 atagtttatt agttttaaat gattgtttta taacaacctc ctttgactaa gacacaaaag
     6361 aaaagccctg ccatctacat gacagggacg aatgtattta tccgcggtac cacccaattt
     6421 cgggccaagc ccgcatcttt agtctttaaa ataaaaagac agaaatattt cattttttca
     6481 gtcattagca accagttttg acacatttgt ggacttctca gcgccgccac tttctgtaaa
     6541 atgtgggatt caaaacacct ctaatagctc tattgtagca agtttttctc ggaaatgcaa
     6601 ggctcaagaa aaattacttg aacttgatgc cattttcttt caatttcttg atggtagctg
     6661 ggccgattcc tttcaaggca aggagttctt tttcagtcca gtttttgaaa tctgaagcag
     6721 acttgattcc ttcatcatag aaagtcttgg cacgatcaag tggaagtcca cccaagtttg
     6781 ctgcaaaatc ttctacagaa ttagctactt tttgagcttg ctctttggta gcttctactg
     6841 cttgttcaac ttttttagaa acagccttac cagctgcgct tgctgactgt tctgcacgct
     6901 tcacgacttt ttttgtctca tctacaacct tagtcacagt acttgaaaag gcacctgcgc
     6961 gacggaggcc atttcgtaat tgttttttac gattgagttt ctttgacatg ataaaatcct
     7021 ttcaataaaa aatccctgct ctgacaagga tttaacataa acattctatc aagattttag
     7081 tgaaaaatca agaatctttc tcgtttaata atttcgctca ccaaacaagc cttctggcaa
     7141 atcatggaat cccttgccta ctttgaagtt ttcaaactta ccaagcaaga actgataagc
     7201 atccaagcga ctctcgtagc gaatcatggc atctttggca cgctcgtcct ggtcttcttc
     7261 gtaaactttt tgactttcct tgtgcgccat gtcgttaatc atgatttggg tattgaccca
     7321 agcttcaaat tccttcataa attcttgttc gtaactcatt tttctcctta ttctaatcca
     7381 cgaaaatagt ccgtatcaat ctcacggtag ggctggatat cctgaacata ctccaacact
     7441 ccttggaatt ctccgtcttc atcgtgtact gcagcatagg tgatgtggac aaacttacct
     7501 cgcgactcag acttgaacca catttcatac ttgtctttgc ttccctcacg aagcctcttc
     7561 atgatagttt tgaccttgtc caagtactta ggcggatggc agagttcgac attgcgcccg
     7621 acttgagacg gcgtccgttt gaaaatcatc tcatcagctg gcgtattgtc attgtaatac
     7681 tggaaaatat cttctttatt gacaaaggta atctccatag ggagatgatt gaggatgaga
     7741 ttggcctgct cgactgaaag atagccatta ccaaaagcct gttgactatg gcggtccagc
     7801 acagcttcct tttccttagg ggtaaaggta atggtaaaat ggccttctgg cgtatctatg
     7861 acttgttgaa cttgaccttc tgccgtatct agctgtacag gctcctctgc aatcttttcc
     7921 tcaataaagc tctgtcgttc tggcacccat ttctctgacg gacggatgat ggcatagcca
     7981 taggcatcgc tctcctccgc aatctgaagc cagtcatcct gagtaaaaga ctcaaggaga
     8041 atcatgagga ggatggactc ttccttgaaa atcatacttt caaactctgt cgcaaaagct
     8101 tcaaaagctt cctttacact gctaattgac acttctggta gtgacttgac tgtcgttaga
     8161 gctgtttgaa agagttccct aatctgatca tccactcccc acataacttt gggaggtgaa
     8221 tcgtgtccat agcgctccat gataggaaag aagagttctt ccttacgttg gtaatggatg
     8281 tcaaattgcc ccacaagccc catctgacgc accaagccct tgcgcatctc cgcaagcatt
     8341 tcctcgtctt ccatcaactc ataagtatct aacaatctac gaatgcgaat caaagctgca
     8401 cggagggcca aattttcttc cttaaagaca cgaactgggt gcccagggtg ttcggtatct
     8461 aaaacttcga cacctttgat agcattttta aaaagattgg catggacatc acagagttcc
     8521 ataacatctt caaaagtgac gcccgaatcc gagttcatca gctcgtgctc cataagggaa
     8581 atctcgatgg ctgacacgcc cgtaaaggtc gcatcaaagc gatcttgaac cgactcagga
     8641 gaggcgccat tgtgcaattc taacaaaata tcccgtagga tatgaatcca ttcatctgtc
     8701 attagtctaa tccaatcact tcgtagccat tcgcttccag tgtgcgtaca atcttgtcca
     8761 taggagttcc tgttagctta gaaccctgtt taagtgatac tttacgacca actgtattgc
     8821 gcattaaggg attggcaagg ggtttaaaac ccaactccac tagaatttcc aagacttctg
     8881 gatgcttgtc caccacttct gcaacaggaa ttgacacatc gatgatattg tccatgacga
     8941 cctccattgt atgttct
//
LOCUS       DAVBCN010000074         8936 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVBCN010000074 DAVBCN010000000
VERSION     DAVBCN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8936
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.074"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..186
                     /locus_tag="VQ497_001710"
     CDS             <1..186
                     /locus_tag="VQ497_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517992.1"
                     /translation="IFTKQIRHWILSDQVISGKRKLFFREDTPKEILDLYENIKSKLD
                     FAYQEVHSNNGLKKYEK"
     gene            351..530
                     /locus_tag="VQ497_001711"
     CDS             351..530
                     /locus_tag="VQ497_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2517993.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            667..846
                     /locus_tag="VQ497_001712"
     CDS             667..846
                     /locus_tag="VQ497_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HET2517994.1"
                     /translation="MPMTQKEMVKLFTAHGWIKTRGGKGSHIKIEKQGERPITILHGE
                     LNKYTERGIGKQAGL"
     gene            883..1335
                     /locus_tag="VQ497_001713"
     CDS             883..1335
                     /locus_tag="VQ497_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HET2517995.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            1626..2096
                     /locus_tag="VQ497_001714"
     CDS             1626..2096
                     /locus_tag="VQ497_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HET2517996.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     VLFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            2115..3203
                     /locus_tag="VQ497_001715"
     CDS             2115..3203
                     /locus_tag="VQ497_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET2517997.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            3184..3612
                     /locus_tag="VQ497_001716"
     CDS             3184..3612
                     /locus_tag="VQ497_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2517998.1"
                     /translation="MELEISDFQGCKIALFCGDKLLTILRDDKDDIPCPNMWELPGGG
                     REGDESPFECARREVYEELGIHLDEDCLLWSKIYPSVIFKGKKSVFMVGQLRQEQFDN
                     IIFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            3750..4700
                     /locus_tag="VQ497_001717"
     CDS             3750..4700
                     /locus_tag="VQ497_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET2517999.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYMGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDMTVVEADLQARLAELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNTG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDKW
                     DMVRQSAESAGFFLETHMIQGEWNACVFKKTKDISGVIGG"
     gene            4702..5445
                     /locus_tag="VQ497_001718"
     CDS             4702..5445
                     /locus_tag="VQ497_001718"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET2518000.1"
                     /translation="MQQYFVKGSAVSPVTIEDRETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFITQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNVVPSIHLFENKADFLAQLDQ
                     FDRILVAYEEAAKEGEAAALIKAVTGLEKGAKLLFIFGPEGGLSPAEIENFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            5456..7252
                     /gene="pepF"
                     /locus_tag="VQ497_001719"
     CDS             5456..7252
                     /gene="pepF"
                     /locus_tag="VQ497_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET2518001.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYGFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVAADEEVLDRLGKLPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDATLTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKHSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIISYSAELGE"
     gene            7498..8052
                     /locus_tag="VQ497_001720"
     CDS             7498..8052
                     /locus_tag="VQ497_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HET2518002.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRNVLLNQGL
                     HYLSKEKDTRGFSSQYGWVHAFAHGADLLTEVVCHPGFPKNRVHEVFEILGQLFKRMS
                     IRFTDDEDWRLARVIYEPILQGKLAQEQVASWIKTVDFPIEEREDFYKFSNFRSCLVE
                     VYVQLDQRNSLQDELKEAIQSFQY"
     gene            8163..8831
                     /locus_tag="VQ497_001721"
     CDS             8163..8831
                     /locus_tag="VQ497_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HET2518003.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLSN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
BASE COUNT         2591 a         1580 c         2131 g         2634 t
ORIGIN      
        1 atttttacaa aacaaattcg tcattggatt cttagtgatc aagttatttc agggaaaaga
       61 aaacttttct tccgggaaga tactccaaaa gagattttag atttgtacga aaacattaaa
      121 tctaaacttg attttgctta ccaagaagtt cattctaata atgggttgaa aaaatatgaa
      181 aaatagaata attgatgttt ttgaagtagt aaatcgcctc cttgtaataa ctgttgaaaa
      241 tcctgatttc gaagacttga gagttaatca tactcaatga aaatcaaaga gcaaactagg
      301 aaactagccg caggctgtac ttgagtacgg taaggcgacg ttgacgtggt ttgaatttga
      361 ttttcgaaga gtatcagttt gtaaaaattg gagataaaaa atatagagta cgcagtggtc
      421 ctatgattca ttcaactccg cctcaatctg tcttagatcg agatactttt acaattgact
      481 atacagatga tgaattgctc gataaagaag cagtttttac tactcattag aaaaataggt
      541 ctgaataaat tggtttccct tagaaccaca tctaggggct tttatgtata atagaataat
      601 aagtatcact cctttacaat acgtgtaaca cgtgttataa tggtgttgtc aggaggtaaa
      661 agcgctatgc ctatgacaca aaaagagatg gttaaactct ttacggccca tggttggata
      721 aagactagag gcggtaaagg ctcccatatt aaaatcgaga agcaagggga aagacctatc
      781 acaatccttc atggtgagct gaataagtac actgaaagag ggataggaaa gcaagctggg
      841 ttgtaaaatt cagtccctgc tttttgatat ggaggtagtg aaatgttagt tacgtatcca
      901 gctttatttt attatgacga cacagacgga acagaagcga cttattttgt ccatttccca
      961 gattttgaat actcagctac acaaggagag gggatttctg aggctttggc tatggggtcg
     1021 gagtggctag ggataactgt tgcagatttg atagagagtg atggagagtt acctcagcca
     1081 tcagatatca atagcttgtc tttaattgat aatgatcctt ttaaagatga tgaagatttc
     1141 gtgtcaacct atgaccttga taaatctttt atttctatgg tatctgttga tgtatcagaa
     1201 tacctaggaa gtcaggaacc cattaaaaag actttgacca taccaaaatg ggcagataag
     1261 ttgggacgag aaatgggact taacttttct cagactttga cagacgctat tgcagataag
     1321 aaagttcaag cctaagatta agcgtggtat cgtggtaata gtcaataaaa aagcacgtcc
     1381 acttgtgcta gttactcgcc tattgatttt atagatttat tagccctttc gagggctttt
     1441 tatattgatt tttatgagaa atgaagaaat cagactttta agaaaatcac tgataccaag
     1501 ggtttaaatg aggtaatatg gtataattag gacataaaat aattttgtgg taagatggta
     1561 gtatctattt tagcatattt ccgagcaatc ggggcgatta aagagtcgca tagaaagagg
     1621 acaaaatggc aacatacgga tttttagatg ttttagagga agagttggac aagaactttc
     1681 cctttgactt tgagattagc tgggacaaac gcaaccatgc ggtagaagtg agttttctat
     1741 tggaagcgca aaatgctgca ggtgtggaga tggtggatga agacggagag gtttcatcag
     1801 atgacattct ttttgaggaa gcggtccttt tctacaatcc tgctaagtca acagtcaatg
     1861 aggaagacta tttgactgtt attccttacc tgcctaaaaa aggattttcc cgtgaatttt
     1921 tagcttattt tgtgcttttc cttaaagata ctgctgaggt tgggctagat gtcctcatgg
     1981 actttttgga agacccagaa gcagaggaat ttgtcatgga atggaaccaa gaagtctttg
     2041 aagaaggaaa agttggcttg gaagagggag aattttatcc ttatccaaga tactaggagt
     2101 tggttggagg tgttatgaag aaaattggga cttatttggt ttatgtgcta gctgttgtct
     2161 ttattatgct agcttttgct tgtggaacaa tcgcatttgc agagttgggg tattccgcag
     2221 ttttagcctt tacttttggt tatgccttcg ctcttctaag catgtattta atctttattc
     2281 ttcatgagct gggtcatgct ttttgtggct acttgacagg ctatcggctg gtggcttttg
     2341 gattaggaca ttttattttg accaaaaagt caggcaggtt tcatcttagt agaacagcca
     2401 ttctgaaaaa tgttggtgct caatacattg gtttaaaaga ggatgaaagc gatcaaagaa
     2461 tcatcctgat gctttcagga ggcttgatgg ttcatctcgg cttgatatta ttggcgatag
     2521 tgtttggatt tttgacaaga agctggtatt ttgcagggac ttggattttt cttaatttat
     2581 ctttcttcct aaataacatt ttgccagtcg acatcaccga tggagcaaaa atttgggaat
     2641 tactacaaca ccctgaaaat accaaatacg cctacctggt attgagacat tctgctcaaa
     2701 ctttgctggc tcctcaagaa tatgatttga aagactttgt tcaagcagtt cctgaggatg
     2761 cacgagggag ttttgcggat ggtgtattag gtatgcaagg tgaagtttcc attttggaag
     2821 ggaaggaaga agtagctaaa cagcagtttc aagcattact tgagaggaca actactccga
     2881 tgatgcagac agttgcccaa ctatccctgc ttcatatcgc cttgttggaa ggagattttg
     2941 agcaagcgga gcaatatgct agtattcgac aggtcaaatc gtttctgtct ctcaaactgt
     3001 ccaatcttca agtgatacaa gcctggtatc aattaaaggt gaagaaagat gtagatcgaa
     3061 ctcgtaaggc tctcaagatt gctagacaga aaatggatag tagtcgaatg ttgagggacg
     3121 agaaacgcta ttatgaaaac ttgttagcag agctggaaaa ggaaatgtta gaaggaactg
     3181 aagatggaac tagaaatttc tgatttccaa ggttgcaaga ttgccctctt ttgtggggat
     3241 aagctactga cgattttgcg tgatgacaag gacgatatcc cttgtcccaa catgtgggag
     3301 ttgccaggtg gtggccgtga aggggacgaa agcccttttg aatgcgctcg gcgtgaagtt
     3361 tatgaggaac tgggaattca tttagatgaa gactgtctgc tttggagcaa gatttatcct
     3421 agcgtaattt ttaaaggcaa gaaatcggtc tttatggtgg gtcagctaag acaagaacaa
     3481 tttgacaata tcatcttcgg agatgaagga cagggctatc agctgatgaa tgttgaggaa
     3541 tttcttagtt ccagtcaagt tgtgcctcag ttgcaagaga gattaaaaga ttatttaaaa
     3601 gtaagtgatt agaaaggatg gttcagttac atttctaaac tgaacccgcc ctaaacactg
     3661 tgccaaaaag ataaacttct cttagacaca agcgtcttca gagaatttcc tattttggtt
     3721 ttgtgtttta cgggcttggt atcttaataa tggaaacatg gcaagagtta aaagttacag
     3781 tgaagcgtga gggagaggag ttggtttcca atctcttgat tgagctggga gcgcaaggag
     3841 tcgcgattga agacagcatg gactatatgg gaaatgtgga tcgttttggc gagattttcc
     3901 cagaggtgga gcagcaagaa gaaatcgtag tgacagccta ctaccctgac acggtggata
     3961 tgacagtggt tgaggcggac ttgcaggctc gcttagcaga attgacggat tttatggatt
     4021 tgggagagtt aaaaataggt acgactgcct tggctgagga agactgggca gacaactgga
     4081 agaaatacta tgaacctgct cgcatcactc atgatttgac catcgttccc tcttggacag
     4141 actatgaggc gactgcggga gaaaaaatta tcaagctgga tcctggcatg gcttttggta
     4201 ctggaaccca cccaactacc aagatgagcc tttttgcctt ggaacaggtt cttcgtggtg
     4261 gcgaaacggt gctagatgtg gggactggtt caggggttct ttctattgca agctcactcc
     4321 taggtgccaa ggagattttc gcctatgact tggatgatgt ggcggttcgt gtcgctcagg
     4381 aaaatattga gctcaacact ggtatggaaa atatccatgt agcagcagga gacttgctta
     4441 agggagttga gattgaagca gatgtcatcg tggctaatat cttggcggat attctcattc
     4501 atctgacgga ggatgcttat cgcttggtaa aggatgaagg ctacctcatc atgagtggca
     4561 ttatcaagga caagtgggac atggtgcgcc agtcggctga gtcagctgga tttttccttg
     4621 aaacccatat gattcaaggg gaatggaatg cctgcgtctt caagaagacc aaggatattt
     4681 caggtgtgat tggaggctag catgcaacag tattttgtca aggggagtgc agtctctcct
     4741 gtcaccatcg aggacaggga aaccagcaag catatgtttc aggttatgcg cttgaaagaa
     4801 gaggatgagg ttaccttagt ctttgatgat ggcatcaagc gcttggcgcg cgtgctggat
     4861 atggaaaatc gtcagtttga gttggtccaa gaattaaatg acaatgtgga actaccagtc
     4921 caagtgacca tcgcatctgg atttcccaag ggagataagt tggagtttat cactcaaaag
     4981 gtgacggaac taggtgccag ccagatttgg gccttccctg ccgattggtc cgttgctaag
     5041 tgggatggca agaaattggg taaaaaggtt gaaaaactag aaaaaattgc tcttggagca
     5101 gcagagcaaa gtaagcgtaa tgttgttcca agtatccatc ttttcgagaa taaagcagac
     5161 tttctagccc aacttgacca gtttgaccgc atcttagtag cctatgaaga ggcggccaaa
     5221 gaaggagaag ccgctgctct tataaaagca gttactggac tcgagaaagg agccaaattg
     5281 ctctttatct ttggtccaga aggtggtctg tcacctgcgg aaattgaaaa ttttgaagct
     5341 aagggagctg ttttggcagg acttggccct cgcattctaa gagcagaaac agcaccgctt
     5401 tacgccttat cagcccttag tgttttagta gaattagaga aataagagga agaaaatgga
     5461 acaaaaacac cgttcagaat ttccagagaa ggaactttgg gatttaacag ctctatacca
     5521 agaccgtgag gatttcttgc gtgcaatcga gaaagctcgc gaagacatca accagtttag
     5581 ccgtgattac aagggcaatc ttcacacttt tgaggatttc gagaaggcct ttgcggaatt
     5641 ggaacagatc tacattcaga tgagccatat tggcaactat ggttttatgc ctcagacgac
     5701 ggactatagc aatgacgaat ttgcaaatat tgctcaagct gggatggaat ttgaaacaga
     5761 tgccagcgta gccttgacct tttttgacga tgccttggtg gcagcagacg aggaagtctt
     5821 ggatcgtttg ggtaaattgc cacatttaac agctgccatt cgtcaggcta aaatcaaaaa
     5881 agcccactac ttaggggcag atgtggagaa ggccttgaca aatctcggtg aagttttcta
     5941 cagtccgcag gacatttata ctaagatgcg agctggggat tttgaaatgg ctgactttga
     6001 agcccatggc aagacctaca aaaacagctt tgtgacctat gagaatttct accaaaacca
     6061 tgaggatgct gaggttcgtg agaaatcctt ccgttccttc tcagagggac ttcgtaagca
     6121 ccaaaatacg gctgcagcag cctatctggc tcaggtcaag tctgaaaaac tcttggctga
     6181 tatgaaggga tacgactctg tctttgacta tcttctagct gaacaagaag tggaccgtgt
     6241 catgtttgac cgccagattg acctcatcat gaaggacttt gcaccagtcg ctcagagata
     6301 cctcaagcat gttgccaagg taaatggtct tgaaaagatg acctttgcag actggaaatt
     6361 ggacttggac agcgccctga atcctgaagt gactattgac gatgcctatg atttggtcat
     6421 gaagtcggta gaacctttgg ggcaagaata ttgtcaggaa gttgctcgtt accaagaaga
     6481 gcgctgggtg gactttgctg ctaacagtgg caaggattcc ggtggttatg cggcggaccc
     6541 atatcgcgta cacccttatg tactcatgag ctggacaggc cgtttgagcg atgtctatac
     6601 cttgattcat gaaatcgggc attctggtca attcatcttt tcagacaatc atcaaagtta
     6661 cttcaatgcc catatgtcga cctactatgt tgaagcaccg tcaaccttca atgaattgct
     6721 actcagtgat tacttggaga accagtctaa tgacccacgt caaaaacgct tcgctctggc
     6781 tcatcgcttg acagacacct acttccataa ctttatcacc cacctcttgg aagccgcctt
     6841 ccagcgtaag gtgtatacat tgattgaaga aggggagacc tttggagcaa gcaagctcaa
     6901 cagcattatg aaggaagttt tgacggattt ctggggagat gctattgaaa ttgacgatga
     6961 tgcaactctg acttggatgc gccaagctca ctactatatg ggcttgtata gttacactta
     7021 ctcagcagga ctagttatct cgactgctgg ttaccttcat ctaaaacatt ctgaaactgg
     7081 agctgaagac tggctcaatc tcctcaaatc aggtggtagc aagacaccac ttgagtcagc
     7141 catgattatc ggtgctgata tttcaacaga caaaccactc cgtgatacca tccaattctt
     7201 gtctgacaca gttgaccaga ttatctccta tagtgctgag ttgggagagt aggggaatag
     7261 aaaaattttt tagaatgatg tttatgggct tgttaagcgt cattctattt gtcttagttg
     7321 tagctatttt caaaaatgtc gttgaagatc gtgatgttaa atttgaactt actgtagaac
     7381 tggtattgat agttatttac gtcattttat atcagattgt atttaattga gaattcaata
     7441 ttgagtcatt taagactgcc tttttgaaca gttgctagaa atgtagaggt attgaccatg
     7501 tatcaagatt tactaagaaa aatagcagaa gaaaaaccaa attataacca ggaagaaatc
     7561 cagtggttgt ttgatcattt gggaaatcct tctccagaaa ttcgtaatgt ccttttaaat
     7621 caaggcttgc actatctttc aaaagaaaag gatacaaggg gtttttcaag tcagtatggt
     7681 tgggttcatg cttttgcaca tggagccgat ttactgacag aggtggtttg tcatccgggc
     7741 tttcctaaaa acagagttca tgaagtattt gagatacttg gccaattatt taaaagaatg
     7801 tcgattcgct ttacagatga tgaggattgg cgtttagcaa gagtgatcta tgaacctatt
     7861 ttacaaggga agttagcgca agaacaagta gcttcttgga taaaaactgt tgattttccg
     7921 atagaagaaa gggaggattt ttacaaattt tccaacttta gatcctgtct ggtggaagtc
     7981 tatgtccaac ttgaccagag aaatagttta caagatgagt tgaaagaagc tatccagtct
     8041 tttcaatact agagattagc taatagttct atgattttca aaaaatttcc aatcaatttt
     8101 cttgaaaccc tttcctactt ttgatagact agtatctagt ttttgaaaaa aggagaaaga
     8161 aaatgaaaaa atttgttgct gagttaatcg gtacgttcat gcttgtgttc gttggaacag
     8221 gagctgttgt ttttggaaat ggtcttgatg gccttggtca ccttggaatc gcctttgcct
     8281 ttggtttggc aatcgtggtg gcagcctact caatcggaac tgtttcaggt gctcacttga
     8341 acccggctgt ttcgattgct atgtttgtaa acaaacgttt gtcatcttca gaacttgtaa
     8401 actacatcct tggtcaggtt gttggagctt tcatcgcttc tggcgcagtc ttcttcctct
     8461 tgagtaattc aggtatgtca actgctagtc ttggtgaaaa tgccttggca aacggtgtca
     8521 ctgtctttgg tggtttcttg tttgaagtca tcgcaacttt cttgtttgta ttggttatca
     8581 tgactgtgac ttcagaaagc aagggcaatg gcgcgattgc tggtttggta atcggtttgt
     8641 cattgatggc gatgattctt gtcggattga agattactgg actttcagta aacccagctc
     8701 gtagcttggc accagctgtc ttggtaggcg gcgcagccct tcaacaagtt tggattttca
     8761 tccttgcacc aatcgctggt ggagttcttg cagcccttgt tgcaaaaaat ttccttggaa
     8821 cagaagaata attgaaactc aaaaagcctt gctcctcatc ttgaggaaca gggctttttc
     8881 gtatgatact cttcgaaaat ctcttcaaac cacgtcagct ttaccttgcc gtaggt
//
LOCUS       DAVBCN010000075         8286 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVBCN010000075 DAVBCN010000000
VERSION     DAVBCN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8286
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.075"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(<1..215)
                     /locus_tag="VQ497_001722"
     CDS             complement(<1..215)
                     /locus_tag="VQ497_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HET2518004.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAARGLQ
                     LGIKTGTWQGAATGAAGGAILGGVAYAA"
     gene            complement(284..553)
                     /gene="blpJ"
                     /locus_tag="VQ497_001723"
     CDS             complement(284..553)
                     /gene="blpJ"
                     /locus_tag="VQ497_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HET2518005.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            complement(1020..1217)
                     /gene="blpI"
                     /locus_tag="VQ497_001724"
     CDS             complement(1020..1217)
                     /gene="blpI"
                     /locus_tag="VQ497_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HET2518006.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            1499..3652
                     /gene="blpA"
                     /locus_tag="VQ497_001725"
     CDS             1499..3652
                     /gene="blpA"
                     /locus_tag="VQ497_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter BlpA"
                     /protein_id="HET2518007.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLVIGDPDPSVKITKMSKERFFSEWTGVAIFLAPKPSYQPHKDKKNGLLSFLPL
                     IFKQKSLIAYIVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVITYIL
                     QQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDANSII
                     DALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFEKMNH
                     DVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYSILQT
                     SLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLLSYFTTPMENIINLQTKLQS
                     AKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDINLTIK
                     QGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINYLPQQ
                     AYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAGLSGG
                     QKQRIALARALLTKSPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRLSIAE
                     RTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            3663..5024
                     /locus_tag="VQ497_001726"
     CDS             3663..5024
                     /locus_tag="VQ497_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HET2518008.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLNQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            5081..5209
                     /gene="blpC"
                     /locus_tag="VQ497_001727"
     CDS             5081..5209
                     /gene="blpC"
                     /locus_tag="VQ497_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HET2518009.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(5280..6620)
                     /locus_tag="VQ497_001728"
     CDS             complement(5280..6620)
                     /locus_tag="VQ497_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2518010.1"
                     /translation="MNVAWILLYTLVTHGLKIVIFFKVDGISLTFERIFKAFLFKILL
                     AVVFGMLGYMVGNTYLSYFMEPLYGIGLSFLLLRELPKKLLLFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDDYSFIWLCIIIFNFFISLAFLKWLDYDFTSLRKGILDKDFQK
                     SLTQINWIMGAYYLVIQNLSYFEYEQGIQSTTVRHLILVFYLLFFMGIIKKLDTYLKD
                     KLHERLNQEQDLRYREMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EIYDSVLKDSSEKLQDNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEGSQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(6634..7371)
                     /locus_tag="VQ497_001729"
     CDS             complement(6634..7371)
                     /locus_tag="VQ497_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2518011.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKEHHITPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANGQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSTRPHRVILYTKTDRLEFTASLEEVLKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            complement(7376..7714)
                     /locus_tag="VQ497_001730"
     CDS             complement(7376..7714)
                     /locus_tag="VQ497_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2518012.1"
                     /translation="MKHMIIQTQKTVYKVNVDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQYGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            7991..>8286
                     /locus_tag="VQ497_001731"
     CDS             7991..>8286
                     /locus_tag="VQ497_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HET2518013.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKT"
BASE COUNT         2718 a         1746 c         1304 g         2518 t
ORIGIN      
        1 gctgcatagg ctacacctcc aagtatagct ccacccgcag caccagttgc tgcaccttgc
       61 catgttcctg ttttaatgcc tagttgaaga cctcttgctg ctgctcctcc aacacctgct
      121 ttggcaaaat ctccccaatt gcatccgcca ccttcaacgc aagcaagcat ttcagtatcc
      181 ataacagaaa attgtgacat catttttgta tccatgacaa atactccttt tttaaaaaac
      241 taaaataaat cagaatagaa tcctcataat tttactataa gtcttaccaa cttagtccca
      301 atttatcacc aaccatacct cctaagcatg ttaatccacc cccaattgca ccaatgtgtg
      361 ctccaacaaa tgcaccagca agtccagcta ctcctaaagt ggccaaacct gctccagttc
      421 caccagttat aattcccgta gtgactcctg taatcagtgc attttgacaa tcagtggagc
      481 tatacccccc ttcaactttc gcaagcattt cagtatccat aacctctaac tgtgacaaca
      541 tttttgtatt catgatgaat acctcctttt tattttcaat ttgttaccaa agtcttaaat
      601 tcaataaaca aatagatttt ttatagtatc tttttgattt tcttaaaaaa gtatatacgt
      661 ctactatctt cttaaaggta gcagtaccta ttttttagtc taagatttca ataatcttga
      721 gtatctaaaa tatcttaatt tcgttattct ccttgcaata aaaagtttta ctatactatt
      781 tattaacttg cagaaagcaa aaaatattag taaataatag tttatagtta agttttttat
      841 tcctaccaat ccatcaacta agtaaaacat caacgattac ataaacgatt gataatataa
      901 ttaaaatttt gctaactatc ttattctcat cattcttaga taactttgat attttgtaag
      961 taagtaaata agacagtaaa ttaatagcga taataatact atatttaaga atcataatct
     1021 tacaaagagg acataattcc tgaacctaca caaataagtg ttgctgctcc cccagttatc
     1081 ggaccagtcg cagcagctaa tagtactgct ccaatacaac caccgattgc agatcctaaa
     1141 ttgcctcttc ctccactaac tatttcgagt tcttcattat ccataacaga aaattgttcc
     1201 atcatttttg tattcatgac aaatactcct tttttctttt tttatttttg tcttgttgta
     1261 actttgataa gtttagtata tcatcgtttt ttaaaatttt tcatccagat cttgaattgt
     1321 catcgaaacg tcttgaatta gcttttttat ttcaagccac ctctaaatgt ttaaaaaaaa
     1381 taatttctaa tcactttttt accattcagg aagttttaat gactattcaa gatttcataa
     1441 aatatgaact tagttttatg acataataga cctatccact atatgaaaag aattgccaat
     1501 gacttcttat aaacgtacat ttgttcctca aatagatgcg agagactgtg gtgtcgctgc
     1561 cttagcctcg attgctaaat tctatggttc agatttttct ctagctcact tgagagaact
     1621 tgcaaagacc aataaagaag ggacgactgc tcttggcatt gtaaaagccg ctgatgaaat
     1681 gggctttgaa acaaggcctg ttcaagcaga taaaactctc tttgacatga gtgatgtccc
     1741 ctatccattt atcgttcacg ttaacaaaga aggaaaactc caacattact atgttgtcta
     1801 tcaaacaaag aaagactatc tggttattgg tgatcctgac ccttctgtaa aaatcactaa
     1861 aatgtcaaaa gaacgctttt tctctgaatg gactggagta gctatttttc tagctcccaa
     1921 acccagctat caaccccata aagataaaaa gaatggtcta ctaagcttcc ttcctctgat
     1981 tttcaaacaa aaatctctca ttgcttacat tgttctctca agcttattgg tcactattat
     2041 caatataggt ggttcttatt atctccaagg aatcttagat gaatacatcc caaatcagat
     2101 gaaatcaact ttaggaatca tctcagttgg tctggttatc acctatatcc tccaacaagt
     2161 catgagcttc tccagagatt atctcctaac cgttctgagc cagagattaa gcattgatgt
     2221 gattttatcc tatattcgcc atatttttga acttcccatg tctttctttg cgacacgtcg
     2281 tacaggagaa atcatttcac ggttcacaga tgctaactct attatagatg ccttggcttc
     2341 taccattctt tctcttttcc tggatgtttc tattctgatt cttgtagggg gcgtcttact
     2401 ggcacaaaac cctaatctct tccttctttc tcttatttct attcctatat acatgttcat
     2461 catcttttct tttatgaagc ctttcgaaaa aatgaaccat gatgtcatgc aaagtaattc
     2521 tatggttagc tctgccatta tcgaagatat caacgggatt gaaactataa agtcgctcac
     2581 gagtgaagaa aatcgctatc aaaatataga cagcgaattt gtagattatt tggaaaaatc
     2641 ctttaagctc agtaaatatt ctattttaca aacaagttta aagcagggaa caaaattagt
     2701 tctgaatatc cttatcctat ggtttggcgc tcaattagtc atgtcgagta aaatttctat
     2761 cggtcagctg attaccttta acacacttct ttcttacttt acaactccta tggaaaatat
     2821 tatcaacctc caaaccaaac tccaatctgc gaaggttgct aataaccgtt tgaacgaagt
     2881 ctatctagtc gaatctgaat ttcaagttca agaaaaccct gttcattcac attttttgat
     2941 gggcgatatt gaatttgatg acctttctta taagtatggt tttggacgag ataccttaac
     3001 agatattaat ctcacgatta aacaaggaga taaggttagc ctagttggag ttagtggttc
     3061 tggtaaaaca actttagcca aaatgattgt caatttcttt gaaccctaca aaggacatat
     3121 ttccatcaat catcaggata ttaaaaacat tgataaaaaa gtcttgcgcc gtcatattaa
     3181 ttacctacca caacaagcct atatctttaa tggctctatc ttggaaaact taactttggg
     3241 cggtaatcat atgattagcc aagaagatat tctaagagct tgtgaattag ctgaaattcg
     3301 tcaagacatt gaaagaatgc ctatgggcta tcaaactcag ctctctgatg gagctggtct
     3361 atcaggagga caaaagcaac gaatagccct cgctcgtgct cttttaacta aatctcctgt
     3421 tttaatacta gacgaagcta ctagcggtct tgatgtcttg actgagaaaa aggttataga
     3481 taatcttatg tctctaactg ataaaaccat tctctttgta gcccatcgtc tcagtatagc
     3541 cgaacgaacc aaccgtgtca ttgttcttga ccaggggaaa atcattgaag ttggtagtca
     3601 ccaagagtta atgcaggcgc aaggtttcta ccatcatcta ttcaataaat aaggagaatg
     3661 tcatgaatcc taatcttttt agaagcgtcg agttttatca gagacgttac cataactatg
     3721 cgacagtgtt aattatacct ctttcattac tatttacttt catcttgatt ttctcccttg
     3781 ttgccacaaa agaaattact gttacttccc aaggagaaat cgcccctaca agtgtcattg
     3841 cctccattca gtcaaccagt gataatccta tcctagctaa tcatttagtg gcaaatcaag
     3901 tagttgaaaa aggggactta ctcatcaaat actctgaaac aatggaagaa agtcagaaaa
     3961 ctgccttagc aactcaatta caaagacttg agaagcaaaa agaaggactt ggaattttga
     4021 aacaaagctt agaaaaagcg actgatcttt tttctggcga ggatgaattt ggctaccata
     4081 atacctttat gaattttact aaacaatccc atgatattga actgggtatc acaaagacta
     4141 acaccgaagt ttcaaatcaa gctaatcttt ccaatagcag ttcatcagct attgaacaag
     4201 aaattacaaa agttcaacaa caaattggag aatatcaaga gttgagagat gctatcataa
     4261 ataacagagc acgcttacca actggcaatc cgcaccagtc aattttgaat cgttatcttg
     4321 tagcctcaca aggacaaaca caaggaactg cagaggagcc atttttatct caaattaatc
     4381 aaagtattgc aggtcttgaa tcatctatcg caagcctcaa aattcagcaa gctggtatcg
     4441 gaagtgtagc aacttatgat aacagtttag caaccaaaat tgaagtactc cgcactcagt
     4501 ttttacagac agcctcacag caacaactaa ctgtggagaa tcaattaaca gaattaaaag
     4561 tacaactaaa tcaagccaca cagcgtttgg aaaacaatac cttaacctcc ccaagtaaag
     4621 gtatcgttca tctgaacagc gaatttgaag gtaaaaatag aattccaact ggtacagaaa
     4681 ttgctcaaat attccctgtc atcacagata caagagaagt actaatcact tactacgtat
     4741 cttctgacta tctacctcta ctagataaag gacaaactgt aagattaaaa ctggagaaga
     4801 ttggaaatca cggcaccacc atcatcggcc aacttcagac aattgatcaa actcctacca
     4861 gaacagagca aggaaatctc tttaaattaa ccgctcttgc aaaactatct aacgaggata
     4921 gtaaactcat ccaatatggc ttacaaggtc gcgtcactag tgtaactaca aagaaaacat
     4981 attttgatta tttcaaagat aaaattttaa cacattctga ttaattttca gataacactc
     5041 tataactatt tattatctta tcaaaaagga gaatcataac atggataaga aacaaaacct
     5101 aacttcattt caagaactaa caactaccga actcaaccaa attacaggtg gaggattgtg
     5161 ggaagattta ttatataaca ttaatagata tgctcattac atcacataag aacttcatca
     5221 tccaatacaa ctataaaaaa ataagaccga gaaacaagta ctctcggtct tatttttcat
     5281 cattctgtat gtatcacagt aagtacctga cgaaagactt gattttgaca ggtggtattt
     5341 agactggtat taggatggct ttccacaatc ttcatgacgg tatagagacc aactcctctc
     5401 tcctcccctt tagaactggc tccaaaggag aagatttcag aaatatcgat gccctcttct
     5461 ttgatggagt tttcgatgat aaaggtctcc tgtgctccat tttttaaaaa ggcgattgaa
     5521 acatgaggtt gactaccttc cacactggct tcaatagcat tgtcacaaag gatagacaca
     5581 atggttagaa aatcaagtag actcatcccc tcgacctgaa tctcctcagg aacttcgaca
     5641 ttaaagacaa tgttcttatc tctagctttt agaaatttcc ctgctaggag acttttgagg
     5701 gctttatcac gaatattcac caatcgacca aggtcatatt tattgtcctg caatttttca
     5761 ctagaatcct ttaagaccga gtcgtagatc tcttttatct gctccatatc ctcctcttca
     5821 atgcccagac gtaagctagt caagaggttg gtgtagtcat ggcgaaaact ccgtacttcc
     5881 ttataaagct cctctatatg ccgactatac cgctccatct ctctgtagcg caagtcctgc
     5941 tcttggttca gtctctcatg gagtttgtcc ttcaaatagg tatccaattt cttgataatc
     6001 cccataaaaa agagcaggta aaagactagg atgagatggc gaacagtcgt tgattgaatc
     6061 ccctgttcat attcaaagta agacagattt tgtatcacca aatagtaagc ccccattatc
     6121 cagttaatct gagtcaggga cttttgaaaa tctttatcga gaatcccctt tctcaagcta
     6181 gtgaaatcat agtccaacca tttcaaaaaa gctagagaaa tgaagaaatt gaaaattatt
     6241 atacataacc aaataaatga gtagtcatca tatacttgcc cttgtcccaa aaatggaagc
     6301 acaaaatagg aaacacctct ataaaagaga ttcactaata tcattggaaa gagaccataa
     6361 aagagaagga gttttttagg aagttctctt aacaataaga aagataagcc tatgccgtac
     6421 aagggttcca taaaataaga tagataagta tttcctacca tatagccaag cattccaaaa
     6481 acaacggcca acagtatctt aaaaagaaag gccttaaaaa tcctctcaaa agtgagacta
     6541 attccatcta ccttaaagaa tatgacaatt tttaatccgt gagtaacaag tgtatacaac
     6601 aatatccaag caacattcat aaactctcct agctcagtgt aagttattaa tagcctcaga
     6661 cacttccctg accttataac gcgcgattag acaacttcca ccattgggaa agaaaagcag
     6721 tttttctttc ttatctaaat gcaccacatt ggcaggattg atgagaaaag agcgatggca
     6781 ctgcaagaga cgaggctcct gcttgagaac ctcctctaga ctcgctgtaa attccagcct
     6841 gtctgtcttg gtatagagaa taacacgatg gggtcttgtg gacgtttcga gatagtaaac
     6901 ctctttaaaa ggatactgga attgagcaaa ttttgattta aagtaaaagc aatcttccgc
     6961 tagactttta ctgtcttgac cattggcata gaggagggct gtctcgatac gagattcaaa
     7021 ctcctctgcc gaaagggcct tatcaatata gtccagagca gacacttggt agcgaaagga
     7081 caggggcata aactccgagt gagtcgtcac aaagacaatc agggcatacg gatcccgatc
     7141 ccgaatcttt ctagccactt ccagaccctt catctcctca tttcgaatct caatatccaa
     7201 aaagaatagc tgatgggccc ccttctcatg cacctctgcc agcagttggt ctggcttgcc
     7261 aaatacctca aaagagctgg gagtgatatg atgttccttc aagagtttct caatcgtcgt
     7321 ttcaatccta gtctgttggg aaaaatcatc ctctaaaaca aatattctca tcttcttact
     7381 ctccctcttt caaccatttt tgacgaattt ctctatagcg acgtctggca tacttgacag
     7441 cgtattgacc ttcttctccg agaaaaagga ttctttcttg aaaatcaatc gattttaatt
     7501 tatcaagatt aaccaaacaa tttcgatgac atctcatcaa ggtttcccca tattggttct
     7561 caagattact taaattttga agcatattaa aactagcttc ttctgtaaca atctgtacgg
     7621 tatgggcttt agttggatgt gtttggatat agtagatatc gtcgacgttt actttataga
     7681 ctgttttctg cgtctgaata atcatatgtt tcattggatt ctcctcctaa gaaaagaata
     7741 acagaaaagt tctagcccaa gcccacccaa tcctaaacag tctttgaaag ctcctaaaag
     7801 gattattttt ctacaacatc atgatgagct tacaactatt ttatcataaa attcactcag
     7861 cgcaattcaa gacatttcaa tgacaattaa agatttgaat aaaaaaaaag aattaaaaat
     7921 gataaactaa cagtatcaaa acacagttgc cagaagtaag actggcaccc agattaaagg
     7981 aggcattccc atgacagata cagaccccat caaaagagct catactttga ttactgactt
     8041 aaacaaagcc taccaagcat gcaaacaggc aagcgctgac gacgtccgct ttcaggagca
     8101 attaaactct attcttggtt ttttagccaa ggctgaaaca gtggataatc gattcttgat
     8161 tgaactagaa aaattttacc agacttccag tcttctcatg ggactcagcg ctcttgatcc
     8221 agatgctcca actcgcgccg cttggcgggc ctatgaccgc ttccactttg accaagtcaa
     8281 gaccaa
//
LOCUS       DAVBCN010000076         8195 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVBCN010000076 DAVBCN010000000
VERSION     DAVBCN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.076"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(135..1103)
                     /locus_tag="VQ497_001732"
     CDS             complement(135..1103)
                     /locus_tag="VQ497_001732"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET2518014.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(1248..2063)
                     /locus_tag="VQ497_001733"
     CDS             complement(1248..2063)
                     /locus_tag="VQ497_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HET2518015.1"
                     /translation="MKKSSILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKKWELPKKVLILSLLGGMFLSGWLSSFTNTYIHDLLGVLFPD
                     SPFLNAFESAIVAPLVEEPLKLLPLVFVLALIPVRKLKSLFLLGIASGLGFQMIEDIG
                     YIRTDLPEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAHKEQKVGKKQGLI
                     FLGLALRTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(2088..2585)
                     /locus_tag="VQ497_001734"
     CDS             complement(2088..2585)
                     /locus_tag="VQ497_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET2518016.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(2658..4019)
                     /gene="radA"
                     /locus_tag="VQ497_001735"
     CDS             complement(2658..4019)
                     /gene="radA"
                     /locus_tag="VQ497_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET2518017.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MSVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(4033..4548)
                     /locus_tag="VQ497_001736"
     CDS             complement(4033..4548)
                     /locus_tag="VQ497_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2518018.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGFPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(4550..4993)
                     /locus_tag="VQ497_001737"
     CDS             complement(4550..4993)
                     /locus_tag="VQ497_001737"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET2518019.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(5050..5147)
                     /gene="ffs"
                     /locus_tag="VQ497_001738"
     ncRNA           complement(5050..5147)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VQ497_001738"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5180..5647)
                     /locus_tag="VQ497_001739"
     CDS             complement(5180..5647)
                     /locus_tag="VQ497_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET2518020.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(5848..7134)
                     /locus_tag="VQ497_001740"
     CDS             complement(5848..7134)
                     /locus_tag="VQ497_001740"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET2518021.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGIGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGARNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(7365..7601)
                     /locus_tag="VQ497_001741"
     CDS             complement(7365..7601)
                     /locus_tag="VQ497_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HET2518022.1"
                     /translation="MLQKIYEQMTNFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            7867..8121
                     /locus_tag="VQ497_001742"
                     /pseudo
     CDS             7867..8121
                     /locus_tag="VQ497_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2613 a         1967 c         1322 g         2293 t
ORIGIN      
        1 tgatgacgta cagtcatatt tcttctcttt ttattatatc acagttttaa atctagcttt
       61 actagattca ccgctactat ctatttattc ggaaaaaaga cgaaaaaacc tgagaatcat
      121 ctcaggcttg gtcattaaat ttttttctca atatcgaaaa gtggagaaag tggtcgtttt
      181 tcatgaatac gtacgatagc atcccctagg agatgagcga ttgaaatctg ctcaatctta
      241 tcaatcaaac gctcttctgg cagatagatg gtatccaaaa caaccaattt cttaatagct
      301 gatttttgga tattgtccgt agcaggacca gaaagaactg ggtgcgtaca gcttgcatag
      361 acttcaacag caccagcttc cgcaagagca tctgccgcat gacaaatcgt tccagcagta
      421 tcaatcatat catcaatcaa gatacaagtc ttgccttcaa ccttaccgat gatgttcata
      481 acttcactag tattcatctt atcaacgcta cgacgtttat caataatagc gatagatgtt
      541 ttcaaaaatt ctgccaactt acgagcacga gtcacccctc catggtccgg gctgacaacc
      601 acatagtcag aaccaaccat accacgacgc tcaaaataat ctgcaatcag aggagcaccc
      661 atcaaatgat ccacaggaat atcaaagaat ccttgaattt gcgcagcatg caagtcgatg
      721 gtcaataaac gatccactcc agctacttca agcatatttg cgacaagttt tgaagtgatt
      781 ggctcacgcg ctctcgcctt tctatcctga cgtgcatacc catagtaagg catgacaaca
      841 ttgacagatt ctgcactcgc acgcttcaaa gcatctacca taatcaaaat ttcaagcaga
      901 ttgtcattta caggcgaact agttgattgt aggataaaga cgtgtttccc acggattgat
      961 tcttcaatgt tgacctgaat ctctccatct gaaaattggc gaacacttga tttccccaac
     1021 tctatcccaa tctcctgcgc cacacgttct gccaattctt tattagaaga aagggcaaac
     1081 agctttaaat cagaaaaaga catgatttcc tccggtatat atgtatcgct tgtgcttttc
     1141 acaagatttt ccatctacca ttgtagcgct ttttgcacta tttttcaatc aaaaataaaa
     1201 gaagggcacc atatttgtac ccttgcatca ttcttttgaa aaatattcta ggtcatcaac
     1261 tcattgtgtt tctcaacaaa gcaataagca tgataaaaac catagagagc aatagccgta
     1321 accactggaa tcgctaaagg caactctgtt tccaactcca caaaaggaga gttaaacaag
     1381 aagtgagttc tcaaggctaa acctagaaaa ataaggccct gtttcttgcc aaccttctgt
     1441 tctttatggg ctctgtaaag caagtaaaca cctactacag ctagacctga aaaagtccag
     1501 tgagaggcaa ttcctgagat gatacgctct aaaattcgcg aaatagtaaa gtcaaagccc
     1561 tctggcaaat ccgtacgaat ataaccaata tcctcaatca tttggaatcc caaaccggaa
     1621 gcaattccaa gtaaaaacaa agattttaat tttcgcacag gaatcaaagc caaaacaaaa
     1681 acaagtggca ataatttcaa gggttcttct accaaaggag ccacaatagc actttcaaag
     1741 gcatttaaaa atggactatc tgggaaaaga acccccagta aatcatggat ataagtatta
     1801 gtaaaactag acaaccagcc tgaaaggaac atccctccca ataaagacag aatcaaaacc
     1861 ttctttggca attcccattt tttcccaata cggaagagaa aataaagagc cggaatcatg
     1921 taaaagagag ctagaaagat agaaactccc attagtccat attccgcacc tgacctcgaa
     1981 ccgtccgtat agtagatggt ttcatactgt aaaccaatac atagcaataa aataaaaata
     2041 aataaaatac tgcttttctt catacacttt ctttctaaat gaagtattta taattctacg
     2101 actgtcatac ttcctgtatc aacattgtaa atggcaccag agataatgac atcgtctggt
     2161 attaggggag actcgataag cagttgcata tcctcgcgta cactctcttc tatatcttgg
     2221 aagggcaaga agtcctggtc tgacacatct acacctaatt cctcttttaa atactcctga
     2281 aaaggttcat tttcaaaggt ctgagcacca cagtctgtat ggtgcaatac cacaatctct
     2341 cttgtcccca tttgttgctg ggaaataact agcgaacgaa tcatgtcttc agtcactcga
     2401 ccacctgcat tccgcaagat atgagcatcc ccaagtgcca agcccagagc ttgcgcaacg
     2461 tgcagacgag agtccataca ggtcacaata gctactcttg ttttgggttt aagtggcaga
     2521 tttaactgcc catgtagggc aacataagcc tgattagctt gcataaactg ttcaaaatac
     2581 gacacgattc cctccttgaa aatttgatag tcaaatattt ctcctatctt atcattttta
     2641 agagaatttg tcacggatta tgcaaagacc tttttcaaaa cttcctgaat cgttgtcacg
     2701 ccaatgacct gaatttcctt aggcagagtg attcctgtca aggaattctt aggtacataa
     2761 atcttagtaa agcccagttt agcagcttcg ttgatgcgtt gctcaatacg attcacgcgc
     2821 cgaatctctc ctgtcaagcc cagttctccg acaaaacatt cctgaggatt agttggcttg
     2881 tctttgtagc tcgaagcaat agcaactgca acagccaagt caatggcagg ttcatccaat
     2941 ttaacaccac cagcagattt gagataggca tcctgatttt gcaagagaag ccctgcccgt
     3001 ttttccaaaa cagacataat caagctagca cggttaaaat caagtcctgt cgtagtacgc
     3061 ttggcatttc caaacatggt cggtgttacc aaagcctgaa cctccgccaa aatcggacgc
     3121 gtcccttcca tggttacaac gatggaggaa ccagtcgccc catccaaacg ctcttctagg
     3181 aaaacttgac tcggattgag tacctcaacc aagccgcccg actgcatctc aaaaatccca
     3241 atctcattag tggaaccaaa acgatttttg accgctctca aaatacgaaa ggtgtggtga
     3301 cgctcccctt caaagtaaag caccgtatcc accatatgct ccaacatacg aggcccagcc
     3361 aaggttcctt ctttggtcac atgacctacg ataaagatgg caatgttatt ggtcttggcc
     3421 aactgcatga gttcagcggt cacttcacgc acctgagaaa cagacccctg cacccctgaa
     3481 atctcaggag acatgatggt ctggatggaa tcaataatga gaaagtctgg ctggatacgc
     3541 tccacttctg cacgaacact ctgcatattg gtctctgcat agagataaaa ctcactatca
     3601 atatcaccta agcgctctgc acgtagttta atctgctggg cagactcctc cccactgaca
     3661 tagagaactg tccccacttg ggacaactgg gttgagactt gtaggagaag agttgatttc
     3721 ccaatcccag gatccccacc gatgaggacg agacttcctg gtaccactcc gcctccaagc
     3781 acacggttga attcctccat ctccgtcttg gttcgattga cattgatgga agtcacctca
     3841 gctagtttca tgggcttggt tttctcacct gtcaaggaca cacgcgcatt cttgacctcg
     3901 gcaacctcaa cctcttccac aaaagaagac caagacccac agttggggca acgtcccaga
     3961 tatttagggg aattataccc acaattttga catacaaatg tcgctttttt ctttgcgatg
     4021 acaaacctct ttctatatct ctaactcaca ctcaatcact tggcaaaaat caatcttctc
     4081 atttggcaca aactggcgca tgagcattcg atgagcaaca actaccacag tctgatgttc
     4141 tcgatactta gacatacatt ctagaaaccg agacttcatt tccgtagctg tctcatattg
     4201 aataggacta ttaggaagca actccccctt gttttctaaa aacagtcttc tagctgtttc
     4261 aaagttttct attcctgttt tatagacctg ccattcatgt aataaaggct ctactcttaa
     4321 aggaaaaccc gtagcacaga ccacatacga agccgtttct aaagctcttg tgactgcaga
     4381 agatacgatt atttcagctg acgagagtaa aggatttttg ctcaatttct ggacttgctg
     4441 ccgtcccatc tcagacaagg gtgccaaatc tatcccaaat cctatataag aacgctcctc
     4501 taactcacgg taatctggct ccccatgacg tacaaagata atcttcattc tagtgccctg
     4561 tcgatccaaa tccaccagtt cgaacgccat cagccgcatc tccatctgca attaagaaag
     4621 tagcaaaaac agcctggaca atacgctccc caacttcaag aacaacctct tggtctgtga
     4681 tattcttcat ctgcgcaaaa atatgccctt catttccagg atttccataa taatccccat
     4741 caatgactcc aactgagtta attaaaacca agcccttctt acgaggattt gaagaacgat
     4801 catagaggta gagaacctca gtcggctgca tataagcctt aacccctgtc ggaaccaaga
     4861 caatctctcc tggcgcaaca actgtacgca cagcaacctt taagtcgtaa ccagccgcat
     4921 gcgctgtctc acgcttgggc aataaatttt catctgtaaa actcgaaacc aattcaaaac
     4981 cacgaatttt cataattttc tcttttctat tatcatttat tctagattat tctatcttat
     5041 ttattcggaa aaagcacgaa aaaaagagca cacaattcaa atcgcttagg gctgctggat
     5101 tcctcccctg acccgcttca cgcagaactg ttgctccact atttattata tcacattcct
     5161 attcatttta aaagcaaaat tattttttcc gtctatttct aaaaaaatcc tgcatgatag
     5221 ctgcgcattc atcttccaaa attcctattt caacctccac acgatgatta agacgctcat
     5281 ctgtcaagat atcgtacaaa cttccagcag cgccaaattt ctggttttta gccccataga
     5341 ccacatttgg aatgcgggca agcccaatcg ccccactaca catgacacaa ggttcaatgg
     5401 tcacaaaaag tgtgcaatcc agcaagcgcc agctctcctc actcaagttc gcatcctcta
     5461 tagccataat ttccgcatgc ataaccgctc gctgtaattc ctcacgcgca ttatgcccac
     5521 gaccaatgat ttccccatct ttgacaatca cacaaccaat tggaatttca tcgtgttcaa
     5581 gagcaatctc agcctctctc aaagcctccc tcataaagac ttctttttct tcaagcgtat
     5641 aatacatctc ttttctcttt tcctacttat cgattttatt attatatcat gaatcccaac
     5701 acaaaaaaag ccaccgaatg cggtgactct atagggagat tattatgaaa aagaaaagtt
     5761 taggatattt gttacaacaa gttaggaggt cttcttgtaa ctgtctatag tatacccgac
     5821 ctatcttaaa caaatcttaa aaatctctta ggaccaaaca ctttctaaaa tatttgtttg
     5881 ttcacgacca ggacctactg agaaagtaga aatacgaacg ccaaccaatt cactcacacg
     5941 acgaacatag ttacgcgcat tctcaggaag atcttccaaa ttgcgagctc cggtaatatc
     6001 ttctgaccaa cctggcaact cttcatagat aggcttgcaa cgtttcaatt gctcaagact
     6061 agctggatag tagtcaatac gttgaccgtc aagatcatag gccacacaaa ttttcacagt
     6121 atccaagccg ctcaaaacat caatagagtt caaagaaagg ttagtaatac cagaaacacg
     6181 acggctatga cgcatcacaa ctgagtcaaa ccaacctaca cgacgtggac gaccagttgt
     6241 tgtaccatat tcatgaccca cttcacggat acgttctccc acttcatcaa acaactcagt
     6301 tgggaaagga ccatctccta cacgactcgt ataagcttta catacaccta caaccttgtc
     6361 aatcttgctt ggaccgatac cagaaccaat tgtcacacca ccagctacag ggtttgatga
     6421 cgtaacaaat ggataagtac cttgatcgat atctagcata acaccttgtg caccttcaaa
     6481 aagcacacgt ttgccattat caagcgcatc attcaagata acagatgtat ctatcacgta
     6541 tttcttgatt tgttgaccat attcgtaata ttcttcaaaa atatcatcga aaacaatcgc
     6601 tttactgtca tacaattttt caaaaagacg attcttttca gcaaggttac gttctaaacg
     6661 ctcacggaaa atatctttat ctaaaagatc tgcaatacga attccaacac gagcagcctt
     6721 gtccatataa gctggaccaa ttcccttaat tgtcgtacca atcttattgt cgcccttagc
     6781 ttcttcttgc aagcgatcca actcgatatg ataaggcaaa ataacatgcg cacgatcaga
     6841 aatacgcaag ttatcagttg ttacaccttc ctcatgaaga tagctcaact cttttacaag
     6901 agatttagga tttacaacca taccattccc aatgacagat attttttcag ggaagaaaat
     6961 cccagatgga atcaagtgca acttaaattt cttaccgtca atcacaatcg tgtgaccagc
     7021 attatcacca ccttggtaac gtgcaattac ttctgcattc gctgaaagga agtctgtaat
     7081 cttcccttta ccttcatcac cccattgggt acctacaaca acaactgaag tcataatttt
     7141 gtctgagccc tcaggctctt cctttctcac atacatggca ggactctcac ctgcaattat
     7201 atcttacaat ttattataat aaaaaatcgc ctttttatca agaagaaaca atagaaagat
     7261 ttgctatttc caactattaa aaaatgattt agaaaaatta ctagctattt actattatct
     7321 ttccataaaa gagtaaatta gttcggaaat ttactaaaat tacctcaaca agaaataaaa
     7381 ccccgattca ttaccaattt ttcaagatac aaacgataag caacacgata atggtaaacg
     7441 ataaaatccc tacgacaacc aatgccatat ctcactaaat aataaattaa aaatttaaaa
     7501 tgaacatgtt cccagtcaaa attatcacca aatgtaggac catactcttc ttcaatacta
     7561 tcatagaaat tagtcatctg ctcataaatt ttttgtaaca taatcaaata ctccttttct
     7621 tttttataaa cttattctaa caaaaaattt tacacattca ctatcaattc ctgaattgtt
     7681 aaaataccag cctcaacaag ataaaaatag gaaaagttga caaaatagaa acaaatttgc
     7741 tacctaaatt tcaaacgatt aaagttctaa aaagaccgcc ttaaaacttt tgggtaaatc
     7801 ctattagaac tataatattt atatatatgt attatggttc tgattttcta gtataaaaaa
     7861 attcaaacta gaattcttct tagaaaaggc cgttttaagt gctatcattg ctcaaaaatg
     7921 gaggtcgctg agacttctat cgtcaagaag aatcatcaaa ttccttgtat catcaaccaa
     7981 aagattgctc agaagttaat tgaaaagact tctatgaccg acattgatca tcagttgtct
     8041 atttcaactt caactgtcat tcgcaagatc aataattttc actttgagca tgatttttcg
     8101 cgtcttcctg agattatgtc ctaggacgtt gaaacagtcc ggggagtgac tgtttcaatc
     8161 gggagatgga gatgagcttt attgctcagg acttt
//
LOCUS       DAVBCN010000077         8175 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVBCN010000077 DAVBCN010000000
VERSION     DAVBCN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.077"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            106..1731
                     /gene="treC"
                     /locus_tag="VQ497_001743"
     CDS             106..1731
                     /gene="treC"
                     /locus_tag="VQ497_001743"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HET2518023.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTGNGIGDFRGIIGKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCIGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNPNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            1873..2121
                     /locus_tag="VQ497_001744"
     CDS             1873..2121
                     /locus_tag="VQ497_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HET2518024.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            2328..3122
                     /gene="racE"
                     /locus_tag="VQ497_001745"
     CDS             2328..3122
                     /gene="racE"
                     /locus_tag="VQ497_001745"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET2518025.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIYVEHVEL"
     gene            3119..4090
                     /locus_tag="VQ497_001746"
     CDS             3119..4090
                     /locus_tag="VQ497_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HET2518026.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGKSSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            4066..4587
                     /locus_tag="VQ497_001747"
     CDS             4066..4587
                     /locus_tag="VQ497_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET2518027.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            4584..5045
                     /locus_tag="VQ497_001748"
     CDS             4584..5045
                     /locus_tag="VQ497_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2518028.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            5036..5776
                     /gene="xerD"
                     /locus_tag="VQ497_001749"
     CDS             5036..5776
                     /gene="xerD"
                     /locus_tag="VQ497_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET2518029.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            5776..6504
                     /locus_tag="VQ497_001750"
     CDS             5776..6504
                     /locus_tag="VQ497_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET2518030.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            6523..7092
                     /locus_tag="VQ497_001751"
     CDS             6523..7092
                     /locus_tag="VQ497_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HET2518031.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKSQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            7079..7801
                     /locus_tag="VQ497_001752"
     CDS             7079..7801
                     /locus_tag="VQ497_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET2518032.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            7801..8043
                     /gene="yidD"
                     /locus_tag="VQ497_001753"
     CDS             7801..8043
                     /gene="yidD"
                     /locus_tag="VQ497_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET2518033.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
BASE COUNT         2364 a         1631 c         1948 g         2232 t
ORIGIN      
        1 ggctagcttc ctagtttgct ctttgatttt cattgagtat aattgaaata gcacaaatag
       61 ggacggaaga ctgcttcctc ccttttatca gaaaaggaga aacagatgac acttgataaa
      121 ggaaaagtag tctatcaaat ctatccaaaa tcttacaaag acaccactgg caatggtatt
      181 ggggatttcc gtgggattat cggaaaaatt ccctatctag ccaaactagg tgtggatatg
      241 gtctggctca atccattcta tccaagtcct caacgggata atggttacga tatttcagat
      301 tatatggcag tggatcctct ttttggtgat atggctgatt ttgaggaaat ggtgtgtatc
      361 ggtaaagagc acaagattga ctttatgctg gacatggtac ttaatcattg ttcgacagag
      421 catgaatggt ttcagaaagc cctagctggt gataagtatt atcaagactt tttcttcatc
      481 caagaccaac caacagattg gcagtctaag tttggtggtt ctgcatgggc gcctttcggg
      541 gatactggga aatactatct tcacctattt gatgagaccc aggctgacct taactggcgc
      601 aatcccaatg tccgtaagga gcttttcaag gttgttaatt tctggcgcga caagggtgtc
      661 aaaggttttc gatttgatgt gatcaatttg attggtaagg acgaggtttc agtggactgt
      721 cctgaaaatg aagggaagcc agcttataca gacaaaccca tcgttcataa ctatttgcgt
      781 atgatgaacc aagccacttt tggatctgac gatagcttta tgacagttgg ggaaatgtct
      841 tctaccacca tggaaaactg tgtcctctat tcgtcgcctg atcgtcagga attatccatg
      901 acctttaatt ttcatcacct caaggtggac tataaggatg gacaaaagtg gaccttggct
      961 ccctttgatt ttgaagagtt gaagagtctt taccatagtt ggggtaagga aatgagtgat
     1021 aaagacggtt ggagtgccct cttttggaac aatcacgatc aaccacgtgc cttgaaccgt
     1081 tttgtcgata ttcaaaactt ccgcaaggaa ggagccacca tgctagcagc cagcattcac
     1141 ctgtcacgtg ggacacctta tatctacatg ggtgaggaaa tcgggatgat tgacccagac
     1201 tatgattcta tggctgatta cgtggatgtc gaatcgctta atgcctatca gatgctccta
     1261 gaagaaggaa agagtcagca agaagccttc cagattattc aagctaagtc gcgtgataat
     1321 tcacgcattc ccatgcagtg ggatgcttcg gaaaatgcag gattttcaac aggcactcct
     1381 tggttgaaag ctggtaaatc ctataaagac atcaatgtag aaaatgaaat ccagggtccg
     1441 atttttacct tctatcaaga cttgattcga ctgcgtaagg aaatgccaat catttcagaa
     1501 ggaagttaca aaccagcctt tgaagatagc aaacaagtct acgcttttga acgccagttt
     1561 gaggatcaaa agttactagt gctcaataac ttttatgcta aagaagtgga aatcgacttg
     1621 ccagcagtct accaaaatgg acagattttg atttcaaact atgaagatgc agaagtgtct
     1681 gagaaaattc tactaaaacc ctatcaaaca ctggctattt atgtaaatta aacttagcaa
     1741 ggagacctct ttttcagaaa agcagggttc tttttgctgt ctattccatc cctgaaagtg
     1801 tagaacaaac tggtcagggt ggctagtttg tggtataatg aaagggactc aaaaagaaac
     1861 aggagaaaaa tgatggattt acttttagca attgtattga ttgtgctagc ttttctagga
     1921 ggagctcttg gaggaatgta cttggttcgt aagcaaatcg aaaaagaatt tgctgacaac
     1981 ccacgtttga atgctgaagc agttcgtact cttttgagtg caaatggtca aaaaccaagc
     2041 gaagctaagg tacaacaagt ttaccaccaa atcatccgcc aacaaaaggc agcccttgct
     2101 aacaataaaa agaaaaaata aataaggaaa agtctgggat gaaagttcca gacttctcac
     2161 tatgttggtt atggtttagg gatgagactt tttccttgca ttctattgat atttgattat
     2221 gattaagaga gaggtagttg acattctatc aagctgtcgg tcagttcgtt cagtacaagg
     2281 aatcataatg aacgatcaat cagaaaaaag actagaaaga agactgtatg gataatcgac
     2341 caattggttt tttggattcg ggtgtcgggg gcttgaccgt tgtgcgcgag ctcatgcgcc
     2401 agcttcccca tgaagaaatc gtctatattg gagattcggc gcgggcgccc tatggccccc
     2461 gtccagctga gcaaattcgt gaatatactt ggcagctggt caactttctc ttgaccaagg
     2521 atgtcaaaat gattgtcatt gcttgtaaca ctgcgactgc ggtcgtctgg gaagaaatca
     2581 aggctcaact agatattcct gtcttgggtg taattttgcc aggagcttcg gcagccatca
     2641 agtccagtca aggtgggaaa atcggagtga ttggaacgcc catgacggta caatcagaca
     2701 tataccgtca gaaaatccat gatctggatc ccgacttaca ggtggagagc ttggcctgtc
     2761 ccaagtttgc tcccttggtt gagtcaggtg ccctgtcaac cagtgttacc aagaaggtgg
     2821 tctatgaaac cctgcgtccc ttggttggaa aggtggatag cctgattttg ggctgtactc
     2881 attatccact ccttcgccct attatccaaa atgtcatggg gccaaaggtt cagctcatcg
     2941 atagtggggc agagtgcgta cgggatatct cagtcttact caattatttt gaaatcaatc
     3001 gtggtcgcga tgctggacca ctccatcacc gtttttacac aacagccagt agccaaagtt
     3061 ttgcacaaat tggtgaagaa tggctggaaa aagagattta tgtggagcat gtagaattat
     3121 gacaaataaa atttatgaat ataaggatga ccaggattgg tatgttgggt cttatagtat
     3181 ttttggtggc gttaacagtt tgagcgacta taagacagat tttcctctgt ttgaattctc
     3241 caaaatattt ggagatgaag agtatggttt cccgctttca gttactgttt tacgctatgg
     3301 ttctacctac cgtttgttct cctttgtggt agacatgctt aatcaagaaa tgggacgaaa
     3361 cttggaagtt attcaacgtc atggggccct gctcttggtt gaaaatgggc aactcttgta
     3421 tgtagaattg cctaaagaag gggtcaatgt tcatgatttc tttgagacaa gcaaggtcag
     3481 agaaaccttg ttgattgcga ctcgtaacga aggcaaaacc aaggaattcc gagctatctt
     3541 tgataagtta ggctacgatg tggaaaatct taatgactac cctgacctgc ctgaagtagc
     3601 agaaacaggt atgacctttg aagaaaatgc ccgccttaag gcagaaacca tttctcaatt
     3661 aacgggcaag atggttttgg cagatgattc tggtctcaaa gtcgatgtcc ttggtggctt
     3721 accaggcgtc tggtcagctc gtttcgcagg tgtgggagca actgaccgtg aaaataatgc
     3781 caaactcttg cacgaattgg ccatggtctt tgaactcaag gaccgctcgg ctcagttcca
     3841 cacaacccta gtcgtagcca gcccaaataa ggaaagttta gttgttgaag cagactggtc
     3901 aggttatatt aactttgaac ctaagggtga aaatggcttt ggctatgatc ccctcttcct
     3961 tgtaggagag acaggtaagt catcagctga attaaccctg gaagaaaaaa atagtcaatc
     4021 tcaccgtgcc ttagccgtta agaaactttt ggaggtattt ccatcatggc aaagcaaacc
     4081 atcattgtaa tgagcgattc ccatggcgat agcttgattg tggaagaagt ccgtgatcgc
     4141 tatgtgggca aagtcgatgc cgtttttcat aacggcgatt ctgaactacg tccggattct
     4201 ccactttggg agggcatccg cgttgttaaa ggaaacatgg acttctacgc cggctaccca
     4261 gaacgtctgg tgactgagct tggttcgacc aagattatcc aaactcatgg tcacttgttt
     4321 gacatcaatt tcaactttca aaagttggac tactgggctc aggaggaaga ggccgctatc
     4381 tgcctctatg gtcacttgca tgtgccaagt gcttggttgg aaggcaagat cctctttcta
     4441 aatccaggtt ctatcagtca accacgaggt accatcagag aatgtctcta tgctcgtgtg
     4501 gagattgatg atagttactt caaagtggac tttttgacac gagatcacga ggtgtatcca
     4561 ggtttgtcca aggagtttag ccgatgattg ccaaggagtt tgagactttc ttgttggggc
     4621 aggaggaaac ttttttgacc cctgctaaaa atctagctgt gttgattgat acccacaatg
     4681 cggatcatgc gaccctcttg ctcagtcaga tgacctatac ccgtgttccc gttgtgacag
     4741 atgaaaaaca gtttgttggg acgattggac tcagagatat tatggcttat cagatggagc
     4801 atgacttgag ccaagaaatc atggcggata cggatatcgt tcatatgaca aaaacggacg
     4861 tagcggttgt ttcgcctgat ttcaccatta cggaggtctt gcacaagcta gtagatgagt
     4921 ccttcttacc ggttgtggat gcagagggta ttttccaagg gattattacg cgtaagtcca
     4981 tcctcaaggc cgttaatgcc ctcttgcatg actttagtaa ggaatatgag attcgatgcc
     5041 aatgagagac aggatttcag cctttttaga ggaaaagcag ggcttgtctg tcaattccaa
     5101 gcagtcctat aagtatgatt tggagcaatt tttagacatg gtaggtgagc ggatttctga
     5161 gaccagtctc aagatttacc aagcccagct agccaatcta aaaatcagcg cccagaagcg
     5221 aaagatttcg gcctgtaacc aatttctata ctttctctat caaaaaggag aggtggacag
     5281 cttttaccgc ttggaattag ccaaacaagc tgaaaagaag acggaaaagc cagagattct
     5341 atacctagac tctttttggc aggaaagcga ccatccagag ggccgcttgc tagcgctctt
     5401 aatcctagaa atggggctct tgcccagtga gattttagcc atcaaggttg cggacatcaa
     5461 tctggatttt caggtgttgc gaatcagcaa ggcttcccaa cagaggattg tcaccattcc
     5521 cacggccttg ctttcagaat tggaaccctt gatggggcag acctatcttt ttgaaagagg
     5581 agagaaaccc tattctcgtc agtgggcctt tcgtcagtta gaatcttttg tcaaggagaa
     5641 aggttttcca tccttatcag ctcaagtctt acgtgaacag tttattctaa gacaaataga
     5701 aaacaaggtc gatttgtacg aaattgcaaa aaaattagga ttaaaaacag tcctgacctt
     5761 agaaaaatat agataatgga tattaaatta aaagattttg aaggccccct ggacttgctc
     5821 ttgcatctgg tttctaagta ccagatggat atctacgatg tgcccattac ggaagtcatc
     5881 gaacagtatc tagcctatgt ttcaaccctg caggccatgc gtctggaagt gacgggtgag
     5941 tacatggtca tggctagtca gctcatgctg attaagagtc gtaaactcct tccgaaggta
     6001 gcagaagtga cagacttggg ggatgacctg gagcaggacc tcctctctca aatcgaagaa
     6061 tatcgcaagt tcaagctctt gggtgagcac ttggaagcca agcaccaaga acgggcccag
     6121 tattattcca aagcgccgac agagttgatt tacgaagatg cggagcttgt gcatgacaag
     6181 acgaccattg acctcttttt ggctttttca aatatcctag ccaagaaaaa agaggagttt
     6241 gcacaaaatc acacgactat cttgcgggat gagtataaga ttgaggacat gatgattatc
     6301 gtgaaagagt ccttgattgg acgagatcaa ttgcgcttgc aggatttgtt caaggaagcc
     6361 cagaatgtcc aagaggtcat caccctcttt ttggcaaccc tagagttaat caaaacccag
     6421 gagctgatcc tcgtgcaaga ggagagtttt ggagatatct atctcatgga aaagaaggaa
     6481 gaaagtcaag tgcctcaaag ctagacttga tagagaggaa agatgagtac tttagcaaaa
     6541 atagaagcgc tcttgtttgt agcgggtgaa gatgggattc gggtccgcca gttagctgaa
     6601 ctcctctctc tgccaccgac aggcatccag cagagtttag gaaaattagc ccagaagtat
     6661 gaaaaggacc cagattccag tttggctttg attgagacaa gtggtgctta tagattggtg
     6721 accaagtctc aatttgcaga gattttgaag gaatactcta aggcgcctat caaccagagc
     6781 ttgtctcggg ctgcccttga gaccttgtcc attattgcct acaaacagcc gattacgcgg
     6841 atagaaattg atgccatccg tggagttaac tcgagtggag ccttggcaaa gttgcaggct
     6901 tttgacctga taaaggaaga cgggaaaaag gaagtattgg ggcgccccaa cctctatgtg
     6961 actacggatt atttcctaga ttacatgggg ataaaccatt tagaagaatt accagtgatt
     7021 gatgagcttg agattcaagc ccaagaaagc caattatttg gtgaaaggat agaagaagat
     7081 gagaatcaat aagtatattg cccacgcagg tgtggccagt aggagaaaag cagaagagct
     7141 gattaagcaa ggcttggtga cggttaacgg ccaagtggtg cgtgaactag caaccactat
     7201 caagtcaggc gacaaggtcg aagttgaagg tcaacctatc tacaacgaag aaaaggtcta
     7261 ctatctgctt aacaaaccac gcggtgtgat ttccagtgtg acagatgata agggtcgcaa
     7321 gacggttgtc gacctcttgc ccaatgtcaa agagcgtatt taccctgtgg gtcgtttgga
     7381 ctgggataca tcaggtgtct tgattttgac caatgatggg gactttacag acgagatgat
     7441 tcaccctcgt aatgagattg acaaggttta tgtcgcgcgt gttaagggtg tggccaataa
     7501 ggacaatctc cgccccttga cccgtggtct tgagattgat ggtaagaaaa ccaagccagc
     7561 tgtttatgaa attctcaaag tggacccagt caaaaatcgc tctgtggtgc agttgaccat
     7621 ccatgaaggg cgtaaccatc aggttaaaaa gatgtttgaa gctgttggtc tccaagtaga
     7681 taagttgtct cggactcgtt tcggacacct agacttgaca ggactccgtc caggagaatc
     7741 ccgtcgtctt aataaaaaag aaatcagcca actacacacc atggctgtaa ctaagaaata
     7801 atgaaacgaa ttttaatagc gcctgtgcgc ttttaccaac gttttatctc accagtcttt
     7861 ccaccctctt gtcgctttga gctgacttgt tccaactaca tgattcaggc tattgaaaaa
     7921 catggtttta agggggtatt gatgggcttg gctcggattt tacgttgtca tccctggtcg
     7981 aaaacaggta aggaccccgt tccagaccac ttttccctta aacgaaatca agaaggggaa
     8041 tgaggtgggg taaatagatt tcaaaatgat aaaaacgcat cctatcaggt ttgagtgaac
     8101 ttgataggat gcgttttaga atgtcaaaat tttatactct tcgaaaatcc cttcaaaccg
     8161 cgtcagcttt catct
//
LOCUS       DAVBCN010000078         8109 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVBCN010000078 DAVBCN010000000
VERSION     DAVBCN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8109
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.078"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            120..497
                     /locus_tag="VQ497_001754"
     CDS             120..497
                     /locus_tag="VQ497_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HET2518034.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIALV
                     YAIAIVDSNYFSDDLAFHSFIVK"
     gene            732..2123
                     /locus_tag="VQ497_001755"
     CDS             732..2123
                     /locus_tag="VQ497_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET2518035.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIDQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            2237..3079
                     /locus_tag="VQ497_001756"
     CDS             2237..3079
                     /locus_tag="VQ497_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET2518036.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQEQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            3352..4113
                     /locus_tag="VQ497_001757"
     CDS             3352..4113
                     /locus_tag="VQ497_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET2518037.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            4483..5400
                     /gene="glyQ"
                     /locus_tag="VQ497_001758"
     CDS             4483..5400
                     /gene="glyQ"
                     /locus_tag="VQ497_001758"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET2518038.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            5660..7696
                     /locus_tag="VQ497_001759"
     CDS             5660..7696
                     /locus_tag="VQ497_001759"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET2518039.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSELFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            7738..7995
                     /locus_tag="VQ497_001760"
     CDS             7738..7995
                     /locus_tag="VQ497_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HET2518040.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
BASE COUNT         2555 a         1431 c         1794 g         2329 t
ORIGIN      
        1 gctttctttt ccgaataaat agatagaagc atagaatcta gcaaacctag atttaaaaat
       61 gtgctataat agaaggagga aaaggaggat tctcagacat ccaggtatca gcccaactaa
      121 tgatttgtca atttatccgc gatatgctgg acttgccagc aaaaaatgtg acgattttgg
      181 agggaagtaa cattcacgtc ttgccttcca tgccctactc agcgcaagat ttctatacta
      241 gtatagacgt cttggcggag ttagataatg gaatccaagt tatcatcgaa attcaggttc
      301 atcatcagaa ttttttcatc aatcgcctat gggcttatct gtgcagtcag gttaatcaaa
      361 acctagaaaa aattcgccaa cgtgaaggtg atacccacca gagctacaaa caaatcgcac
      421 tagtatacgc tatcgcaatt gtcgatagta attacttctc agatgaccta gcttttcata
      481 gttttatagt aaaatgaaat gagaacagga caaatcgatc aggacagtca aatcgatttc
      541 taacaatgtt ttagaagtat aggtctacta ttctagcttc aatctactag aaattccata
      601 gatagaaaac tacataatct ctacagatac ggatgttgga gttgatgtaa gatgctttgg
      661 cttgctagag gaattgtgga ttgccaaatt gtatcattga aattattgct caaatttgtt
      721 atgatataaa tatgaataaa agtagactag gacgtggcag acacgggaaa acgagacatg
      781 tattattggc tttgattggt attttagcaa tttctatttg cctattaggc ggatttattg
      841 cttttaagat ctaccagcaa aaaagttttg agcaaaagat tgaatcgctc aaaaaagaga
      901 aagatgatca attgagtgag ggaaatcaga aggagcattt tcgtcagggg caagccgaag
      961 tgattgccta ttatcctctc caaggggaga aagtgatttc ctctgttagg gagctgataa
     1021 atcaagatgt taaggacaag ctagaaagta aggacaatct tgttttctac tatacagagc
     1081 aagaagagtc aggtttaaag ggagtcgtta atcgtaatgt gaccaaacaa atctatgatt
     1141 tagttgcttt taagattgaa gagactgaaa agaccagtct aggaaaggtt cacttaacag
     1201 aagatgggca accttttaca cttgaccaac tgttttcaga tgctagtaag gctaaggaac
     1261 agctgataaa agagttgacc tccttcatag aggataaaaa aatagatcaa gaccagagtg
     1321 agcagattgt aaaaaacttc tctgaccaag acttgtctgc atggaatttt gattacaagg
     1381 atagtcagat tatcctttat ccaagtcctg tggttgaaaa tttagaagag atagccttgc
     1441 cagtatctgc tttctttgat gttatccaat cttcgtactt actcgaaaaa gatgcggcct
     1501 tgtaccaatc ttactttgat aagaaacatc aaaaagttgt cgctctaacc tttgatgatg
     1561 gtccaaatcc agcaacgacc ccgcaggtat tagagaccct agctaaatat gatattaaag
     1621 cgactttctt tgtgcttggg aaaaatgttt ctgggaatga ggacttggtg aagaggataa
     1681 aatctgaagg tcatgttgtt ggaaaccata gttggagcca tccgattctc tcgcaactct
     1741 ctcttgatga agctaaaaag cagattactg atactgagga tgtgctaact aaagtgctgg
     1801 gttctagttc taaactcatg cgtccacctt atggtgctat tacagatgat attcgcaata
     1861 gcttggattt gagctttatc atgtgggatg tggatagtct ggactggaag agtaaaaatg
     1921 aagcatctat tttgacagaa attcagcatc aagtagctaa cggctctatc gttttgatgc
     1981 atgatattca cagtccgaca gtcaatgcct tgccaagggt cattgagtat ttgaaaaatc
     2041 aaggttatac ctttgtgacc ataccagaga tgctcaatac tcgcctaaaa gctcatgagc
     2101 tgtactatag tcgtgatgaa taagcaagaa aaaataggtc tgttagatat ttgacagact
     2161 tattttttac agaatatagt actacttaaa aaatgtttta tgctataatt gatgaataaa
     2221 atagaaggag aagcatatga atacctatca attaaataat ggagtagaaa ttccagtatt
     2281 gggatttgga acttttaagg ctaaggatgg agaagaagcc tatcgtgcag tgttagaagc
     2341 cttgaaggct ggttatcgtc atattgatac ggcggcgatt tatcagaatg aagaaagtgt
     2401 tggtcaagca atcaaagata gcggagttcc acgtgaagaa atgttcgtaa ctaccaagct
     2461 ttggaatagt cagcaaacct atgagcaaac tcgtcaagct ttggaaaaat ctatagaaaa
     2521 actgggcttg gattatttgg atttgtattt gattcattgg ccgaacccaa aaccgctcag
     2581 agaaaatgac gcatggaaaa ctcgcaatgc ggaagtttgg agagcgatgg aagacctcta
     2641 tcaagaaggg aaaatccgtg ctatcggcgt tagcaatttt cttccccatc atttggatgc
     2701 cttgcttgaa actgcaacta tcgttcctgc ggtcaatcaa gttcgcttgg cgccaggtgt
     2761 gtatcaagaa caagtcgtag cttactgtcg tgaaaaggga attttattgg aagcttgggg
     2821 gccttttggt cagggagaac tatttgatag caagcaagtg caagaaatcg cagcaaatca
     2881 cggaaaatcg attgctcaga tagccttggc ctggagcttg gcagaaggat ttttaccact
     2941 tccaaaatct gtcacaacct ctcgtattca agctaatctt gattgctttg gaattgaact
     3001 gagtcatgag gagagagaaa ccttaaaaac gattgctgtt caatcgggtg ctccacgagt
     3061 tgatgatgtg gatttctaga aaatcataaa aagaattgta cattattcta atttttgata
     3121 taatagtcag caggaaagaa agtcttatgg cgttcttcaa gcgagcttgg gatagtggga
     3181 gccaagtagg gcaaaataat gagctggcgc tttctgtagt attttcaaaa acaatgaagt
     3241 aataaattag ggtggaaccg cgtttctgac gcccctaggt taaatcaacc taggattgtc
     3301 ggacgcggtt cttttgctta ttcagtctat tgtgtgaaag aaaggagagc cgtggacaac
     3361 ctttatcttg taaaagacga tagtcaacta gctacatttc gtgattttgt agtaagaaat
     3421 actgaaaagt tgaaagatta tcaatctttt ttaaagaatg aacttgcagt ctgtgattta
     3481 ccgcaagccg ttatttggtc agattttaat gctgctacac agattattag ggaaagcgct
     3541 gttccagcct atacaaataa tagacgaatg gttatggcgc ctgatttagc tgtttggaaa
     3601 gagttgtatt tgtatcaatt gatggactac gagtgttctc agcaaactca agcaatagaa
     3661 agtcactatc attctttatc tgaaaatttc ctcttacaga ttgtaggaca tgagttagct
     3721 cattggtcgg agcatttttt agatgatttt gatggttatg actcttatat ctggttcgaa
     3781 gaggggatgg ttgaatatat tagtcgcaag tatttcttga cagaagagga atttcaagcg
     3841 gaaaaaattt gtaatcaatc tctcgtagaa ctttttcaga agaagtatgg ttggcattca
     3901 ttgaatgatt ttggttcttc gacttatgat aagaactatg caagtatttt ttatgaatac
     3961 tggcgcagct ttttgacagt agataagttg gtagaaaatt taggtagtgt acaagcggtc
     4021 ttagattctt atcatttatg ggcaaataca gaaaaaactt ttcccttgtt agattggttt
     4081 gttcagcaga aattaattga aaaagaaata taaaaactaa aggagtaaac aatgtctaag
     4141 aaattgacat ttcactgcgt cagtggcaga aacctcctta cagtcggact gccccacgct
     4201 cagcactaga gtgcctgagc tagacgcagt actaactcgt cttgcctcgt atgatcgacg
     4261 aggcagactc atgtcgcaag taattatttt ttattaagga gtaaacaatg tctaagaaat
     4321 taacatttca ctgcatcagt ggcagaaacc tccttacagt cggactgccc cacgctcagc
     4381 accctagtgc tgagctagac gcagtactaa ctcgtcttgc ctcgtataat cgacgaggca
     4441 gactcgtgtc gcaagaaatc attttttatt aaggagtaaa caatgtctaa gaaattaaca
     4501 tttcaagaaa ttattttgac tttgcaacaa ttttggaatg accaaggttg tatgcttatg
     4561 caggcttatg acaatgaaaa aggtgcgggg acaatgagtc cttacacttt ccttcgtgct
     4621 atcggacctg agccatggaa tgcagcttat gtagagccat cacgtcgtcc tgctgacggt
     4681 cgttatgggg aaaaccctaa ccgtctctac caacaccacc aattccaggt ggtcatgaag
     4741 ccttctccat caaatatcca agaactttac cttgagtctt tggaaaaatt gggaatcaat
     4801 cctttggagc acgatattcg ttttgttgag gacaactggg aaaacccatc aactggttca
     4861 gctggtcttg gttgggaagt ttggcttgac ggaatggaaa tcactcagtt cacttatttc
     4921 caacaagtcg gtggattggc aactggccct gtgactgcgg aagttaccta tggtttggag
     4981 cgcttggctt cttacattca agaagtagac tctgtctatg atatcgagtg ggctgatggt
     5041 gtaaaatacg gagaaatctt tatccagcct gagtatgagc actcaaaata ttcatttgaa
     5101 atttcggacc aagaaatgtt gcttgaaaac tttgataagt ttgaaaaaga agctggtcgt
     5161 gcattagaag aaggcttggt acaccctgcc tatgactatg ttctcaaatg ttcacatacc
     5221 tttaatctgc ttgacgcgcg tggtgccgta tctgtaacag agcgtgcagg ctatatcgct
     5281 cgtatccgta acttggcccg tgttgtagcc aaaacctttg ttgcagaacg caaacgccta
     5341 ggttacccac ttttggatga agaaacacga gttaaacttt tagcagaaga cgcagaatag
     5401 tgagtaatac tgtactctaa aatcgttaga ggcaagtcga agacttgcta gagggatatg
     5461 caccgccgta ctgttatgtg gggatttttg aaaccttagg ttcaaaaatc agtcagctaa
     5521 atccactttg atgagattgt tggaaatctt gataaattta gcattggatt tccatacaga
     5581 ctcacagagt tagttagcga cgtccccagt acctaaggtg catatcaaaa aagattgaag
     5641 aaaatgtaaa ggaaaaaaca tgacaaaaaa cttattagta gaactcggtc ttgaagaatt
     5701 accagcctat gttgtcacac caagtgaaaa acaactaggc gaaaaaatgg cagccttcct
     5761 caaggaaaac cgcctgtctt ttgaagccat tcaaaccttc tcaacaccac gtcgtttggc
     5821 tgttcgtgtg actggtttat cagacaaaca gtctgatttg acagaagatt tcaagggacc
     5881 agcaaagaaa attgccttgg atagtgatgg aaactttacc aaagcagctc aaggatttgt
     5941 ccgtggaaaa ggcttgactg ttgaagatat cgaattccgt gaaatcaagg gtgaagaata
     6001 tgtctatgtc actaaggaag aagttggtca agcagttgaa gccattgttc ctggtgttgt
     6061 agatgtcttg aagtcattga ctttccctgt cagcatgcac tgggctggaa atagctttga
     6121 atacattcgt cctgtccaca ctttaactgt tctcttggat gagcaagagt ttgacttgga
     6181 tttccttgat atcaagggaa gtcgtgtgag ccgtggccat cgtttcttgg ggaaagaaac
     6241 caaaattcag tcagcattga gctatgaaga agaccttcgt aagcagtttg taatcgcaga
     6301 tccatgtgaa cgtgagcaaa tgattgttga ccaaatcaag gaaattgagg caaaacatgg
     6361 tgtacgtatc gaaattgatg cggatttgct gaatgaagtc ttgaatttgg ttgaataccc
     6421 aactgccttc atgggaagtt ttgatgctaa ataccttgaa gttccagaag aagtcttggt
     6481 gacttctatg aaggaacacc agcgttactt tgttgttcgt gatcaagatg gaaaactctt
     6541 gccaaacttc atttctgttc gtaacggaaa cgcagagcgt ttgaaaaatg tcattaaagg
     6601 aaatgaaaaa gtcttggtag cccgcttgga agacggagaa ttcttctggc gtgaagacca
     6661 aaaattggtg atttcagatc ttgttgaaaa attaaacaat gtcaccttcc atgagaagat
     6721 tggttccctt cgtgaacaca tgattcgtac gggtcaaatc actgtacttt tggcagaaaa
     6781 agctggtttg tcagtggatg aaacagttga ccttgctcgt gcagcagcca tttacaagtt
     6841 tgacttattg acaggtatgg ttggtgaatt tgacgaactc caaggaatta tgggtgaaaa
     6901 atacaccctt cttgctggtg aaactccagc ggtggcagct gctattcgtg aacactacat
     6961 gcctacatca gctgaaggag aacttccaga gagcaaggtc ggcgcagttc tagccattgc
     7021 agacaaattg gatacgattt tgagtttctt ctcagtagga ttgattccat caggttctaa
     7081 tgacccttat gcccttcgtc gtgcaactca aggtgtggtt cgtatcttgg atgcctttgg
     7141 ttggcacatt gctatggatg agctgattga tagcctttat gcattgaaat ttgacagttt
     7201 gacttatgaa aataaagcag aggttatgga ctttatcaag gctcgtgttg ataagatgat
     7261 gggctctact ccaaaagata tcaaggaagc agttcttgca ggttcaaact ttgttgtggc
     7321 agatatgttg gaagcagcaa gtgctctcgt agaagtaagc aaggaagaag attttaaacc
     7381 atctgttgaa tcactttctc gtgcctttaa cctggctgag aaggcagaag gggttgctac
     7441 agttgattca gaactatttg agaatgacca agaaaaagct ttggcagaag cagtagaaac
     7501 actcgtttta tcaggacctg caagtcagca attgaaacaa ctctttgcgc ttagcccagt
     7561 cattgatgct ttctttgaaa atactatggt aatggctgaa gatcaggctg tccgtcaaaa
     7621 tcgtttggca atcttgtcac aactaaccaa gaaagcagct aagtttgctt gttttaacca
     7681 aattaacact aaataaaatt tgataaacgg actttatctt attacaaagg agaagaaatg
     7741 gatccgaaaa aaattgctcg tatcaatgag cttgctaaaa agaaaaaaac agaaggctta
     7801 acaccagaag aaaaagtgga acaagccaaa ctacgtgagg agtacatcga aggttatcgc
     7861 cgcgctgttc gtcaccacat tgaaggaatc aaaattgtgg acgaagaagg aaacgatgtt
     7921 acaccagaaa aactacgcca agtacaacgt gaaaaaggat tacatggccg tagtcttgat
     7981 gacccaaatt cataatatgt actctttctt ttaacaaaga tagatgaaac gataacaaag
     8041 agactagcag tttgtgtttg ctagtctttt ttcgctaaaa aaggaaccat aatggttcct
     8101 aaaaactat
//
LOCUS       DAVBCN010000079         7948 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVBCN010000079 DAVBCN010000000
VERSION     DAVBCN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7948
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.079"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            16..1965
                     /gene="mutL"
                     /locus_tag="VQ497_001761"
     CDS             16..1965
                     /gene="mutL"
                     /locus_tag="VQ497_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET2518041.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKIQITDNGYGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(2290..2757)
                     /locus_tag="VQ497_001762"
     CDS             complement(2290..2757)
                     /locus_tag="VQ497_001762"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET2518042.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(2758..3963)
                     /locus_tag="VQ497_001763"
     CDS             complement(2758..3963)
                     /locus_tag="VQ497_001763"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET2518043.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(3983..4618)
                     /gene="ribE"
                     /locus_tag="VQ497_001764"
     CDS             complement(3983..4618)
                     /gene="ribE"
                     /locus_tag="VQ497_001764"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET2518044.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(4603..5703)
                     /gene="ribD"
                     /locus_tag="VQ497_001765"
     CDS             complement(4603..5703)
                     /gene="ribD"
                     /locus_tag="VQ497_001765"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET2518045.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(5838..5952)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6109..6702
                     /gene="ruvA"
                     /locus_tag="VQ497_001766"
     CDS             6109..6702
                     /gene="ruvA"
                     /locus_tag="VQ497_001766"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET2518046.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            6712..7275
                     /locus_tag="VQ497_001767"
     CDS             6712..7275
                     /locus_tag="VQ497_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET2518047.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRDFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            7272..>7948
                     /locus_tag="VQ497_001768"
     CDS             7272..>7948
                     /locus_tag="VQ497_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2518048.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     VGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
BASE COUNT         2260 a         1594 c         1541 g         2553 t
ORIGIN      
        1 gaaaggggct gaaagatgtc tcatattatt gaattgccag agatgctggc aaaccaaatc
       61 gcagctggag aggtcattga acgtcctgcc agcgtggtca aagaattggt agaaaatgcc
      121 attgacgcgg gctctagtca gattatcatt gagattgagg aagctggtct caagaaaatc
      181 caaatcacag ataacggtta tggaattgcc cacgatgagg tggagttggc cctgcgtcgc
      241 catgcgacca gtaagataaa aaatcaagca gatctctttc ggattcggac gcttggtttt
      301 cgtggtgaag ccttgccttc tattgcgtct gttagtgtct tgactctgtt aacggcggtg
      361 gatggtgcta gtcatggaac caagttagtc gcgcgtgggg gtgaagttga ggaagtcatc
      421 ccagcgacta gtcctgtggg aaccaaggtt tgtgtggagg atctcttttt caacacgcct
      481 gcccgtctca agtatatgaa gagccagcaa gcggagttgt ctcatatcat tgatattgtc
      541 aaccgtctgg gcttggccca tcctgagatt tcttttagct tgattagtga tggcaaggaa
      601 atgacgcgga cagcagggac tggtcaattg cgccaagcaa tcgcagggat ttacggtttg
      661 gtcagtgcca agaagatgat tgaaattgag aactctgacc tagatttcga aatttcaggt
      721 tttgtgtcct tgcctgagtt gactcgggct aaccgcaatt atatcagcct cttcatcaat
      781 ggtcgttata tcaagaactt cctgctcaat cgtgctattt tggatggttt cggaagcaag
      841 cttatggttg gacgttttcc actggctgtc attcacatcc atatcgaccc ttatctagcg
      901 gatgtcaatg tgcatccaac taagcaagag gtgcggattt ccaaggaaaa agaactgatg
      961 accctggttt cagaagctat tgcaaatagt ctcaaggaac aaaccttgat tccagatgcc
     1021 ttggaaaatc ttgccaaatc gactgtgcgc aatcgtgaga aggtggagca aactattctc
     1081 ccactcaaag aaaatacgct ctactatgag aaaactgagc cgtcaagacc tagtcaaact
     1141 gaagtagctg attatcaggt agaattgact gatgaagggc aggatttgac cctgtttgcc
     1201 aaggaaacct tggaccgatt gaccaagcca gcaaaactgc attttgcaga gagaaagcct
     1261 gctaactacg accagctaga ccatccagag ttagatcttg ctagcatcga taaggcttat
     1321 gacaaactgg agcgagaaga agcatccagc ttcccagagt tggagttttt cggacaaatg
     1381 cacgggactt atctctttgc ccaagggcga gatggacttt acatcataga tcagcacgct
     1441 gctcaggaac gggtcaagta cgaggagtac cgtgaaagca ttggcaatgt tgaccagagc
     1501 cagcagcaac tcctagtgcc ctatatcttt gaatttcctg cggatgatgc cctgcgtctc
     1561 aaggaaagaa tgcctctctt agaggaagtg ggcgtctttc tagcagagta cggagaaaat
     1621 caatttattc tacgtgaaca tcctatttgg atggcagaag aagagattga atcaggcatc
     1681 tatgagatgt gcgacatgct ccttttgacc aaggaagttt ctatcaagaa ataccgagca
     1741 gagctggcta tcatgatgtc ttgcaagcga tctatcaagg ccaatcatcg tattgatgat
     1801 cattcagcta gacaactcct ctatcagctt tctcaatgtg acaatcccta taactgtcct
     1861 cacggacgtc ctgttttggt gcattttacc aagtcggata tggaaaagat gttccgacgt
     1921 attcaggaaa atcacaccag tctccgtgag ttggggaaat attaaaagta taaaaaagtc
     1981 tgggaaaaat tttcaaaatc agaaaaacgc ataaaatcag gtgttcaaaa accttgattt
     2041 tatgcgtttt atcatggaaa tagttacttc atgttttcct aattcttttc gaaactcttt
     2101 ttaaaccacg tcagttttat cagtaatctc aaaacagtgt tttgagctaa ttttgccagt
     2161 tttatctgta acatcgaagt tgtgtttgac cactctgcga ctggtttcct agtttgctct
     2221 atgattttca cagagtatta aattgcgatt ttgccaagtt tctttattcg tctaaaagta
     2281 gagtctgttc tatgcgtcta atgtacgaat taggttgacc atttcaatag ctccttgtgc
     2341 acactcagaa cccttatttc ctgctttggt accagctcgt tctatggctt gttcaattgt
     2401 atctgtcgtt agcacaccaa acataacagg aatttcgcta tttaaactga tttgggcgat
     2461 tcccttagat acctcgctac atacataatc ataatgactt gtattccctc taatgacagc
     2521 tcccaagcag ataattgcat catatttttt actttttgcc atttttgatg caatcagtgg
     2581 tatttcaaaa gctcctggaa cccaggctac ctctatatct ttctcgttta cattctctct
     2641 tttgagatta tctagtgctc cagataataa ttttgaagtt ataaattcat taaatctcgc
     2701 tacaacaata cctattttaa tattgtttgc tactaaatta ccttcataag tgttcattta
     2761 tttttcctcc atatttaaaa tgtgacccat tcgatttttc tttgtttcta aataaaaact
     2821 atcgtaagga ttggcttcta tttcgattga tattctactg gaaatggtaa ttccatattt
     2881 ttctaactgt tcaaccttgt caggattatt tgtcagtaaa tgaagtgact gaagtcccag
     2941 atctttaagc atttgtgctc caatatgata ttctcttaaa tcaccttcaa agcctaatgc
     3001 aagattggca tcaagcgtat ccatgccttg atcttgtaaa tgataggctt ttaatttatt
     3061 gataagtcca attcctcgtc cctcctgtcg caagtaaagt aagacacccg aaccattctc
     3121 aacaatcatt ttcatagctt tatcgaattg ctgtccacaa tcgcaacgta aagagcctaa
     3181 aacatctcct gttaaacatt cggagtggac ccgacataat acattggctt catcctctat
     3241 atttcccata ataagagcaa gatgatgttc cccatttagt ttatctatat agctaattgc
     3301 tttgaaatta ccgtatctag taggcatatt gacagttgaa actcgttcta ccagctgatc
     3361 atatactttt ctatattctt gtaattcttt gatggtaatt agtggaatgt tgtgtttctt
     3421 cgagaactga attaaatcat ctgttctcat cattttgcca tcatgattca ttatttcaca
     3481 acataggcca cactctttta gtccagctaa ttttaataaa tcaacagttg cttctgtgtg
     3541 tccatttctt tctagaacac cacctttttt tgcaattaaa ggaaacatgt gtcctggcct
     3601 gcgaaaatca gagggtgtta tatcttcagc tacacacata cgtgcggtca gtcctctctc
     3661 ctcggcagaa atacctgtgg tcgtttcttt ataatcaatt gaaactgtaa aagcagtctt
     3721 atgattatct gtattgtttt caaccatagg tgaaagcatt aattgattag ctaaactttc
     3781 gctcataggc atacaaatta atcctttggc ataagtagcc ataaaattaa cattttctgt
     3841 tgtagctgct tgtgcagaac aaattaagtc tccttcattt tctctatcct tgtcgtctat
     3901 aacaagaaca agtcgtccct tctgcaatgc ttctaatgct tcttgtattt ttcgatattc
     3961 cattgactga ttatcctttc tgctaaaatc cattttgata taatagttcc ttagatattt
     4021 ctgattttgg agagttatcc atcagttttt gcacatattt acctaagata tcattttcaa
     4081 gatttactgt actcccgact tgtttactct taagaatggt ttgttccaag gtatgaggga
     4141 taacagatac tgaaaagttt actttggaga ctttagcgac agtcagacta atgccgtcaa
     4201 ttgtaataga tcctttttca actattaaat ctaaaatttc tttttgtgtg ttgatttgat
     4261 accatacagc attatcatct ttttttattg acgagatttt tcctgtacca tcaatgtgtc
     4321 ctgtaacgac gtgaccccca agtcgaccgt tgacagataa ggctctttct agattcacct
     4381 cacttccatg ttttaataga gtaagagctg ttcgactcca tgtttcattc attacatcaa
     4441 ctgtaaagga ttgatgattg aaatgagtaa ctgtaagaca gataccattt actgctatac
     4501 tatcgcctaa atggatatcc gttaatattt ttgaggcttt aattgatagt ttacaattac
     4561 gagagtcttt ctgtattctt tcaacttttc cgatttcttc aattattcct gtgaacatgg
     4621 ataaatcact tcactttcta tgagatagtc atttcctatt tgagaaaaag cataaggttt
     4681 caatctaata gcgtcatttg gcaaagaaat gccttcacct ccgacaggaa acttggcact
     4741 gcctccaaaa atttttggtg caatatatat tttcagctca tcaacaattt gttgttccaa
     4801 agcactccaa ttcattagac tgcccccttc tagaactagg ctatcaatct gcatgtttcc
     4861 tagatgttgc attaaactcg ataagtctat atgattgcct tttttcttta tggaaagtat
     4921 ttcacagcca tgattttgat atagcttcat tttatttttg tcttcagagg aagtggcaat
     4981 gtaagtttta atatcatttg ctgtttttac gattttagag gtaagaggag ttcgtaaatg
     5041 tgtatcgcat atgatacgga taggattttt cccttcctcc aatctacatg tcagcaaagg
     5101 atcgtcttga ataacagtat tgactcccac cataattgca ctaacatggt gtcgtaactg
     5161 atgcacatgc tttcttgctt cttcttcagt aatccatttg gattgatttg ttttagtggc
     5221 tatttttcca tccattgaca ttgcatattt cataaaaaca tagggtacat gctgggtaat
     5281 atactttcta aaacttttta ttaagttaag acactcattt tctaaaattc caacagtaac
     5341 ttgaagatta ttttcctcaa gtatctttac tccttttcca gatacaatag gattacagtc
     5401 taggcttcca atgactactc ttgtaatacc actatcgatt atagcatcta tacagggagg
     5461 tgttttcccg aagtgacaac agggttcaag tgttacataa agcgtcgctc cgacagggga
     5521 ttctctacag tttttaagag catttctctc agcatgtggg ccaccaaaaa actcatgata
     5581 accttgtccg atgatgtgat tatcttttac aataactgcg ccgaccatag gattgggatt
     5641 gacgtaacca gccccttttt gtgccagttt tattgctaat ttcatatatt ttgaatcgct
     5701 catctcacta cctccaaaaa aaatatacct tgaatagggg actactcaag gcatacaaaa
     5761 gaaaacttat gcgattaaca aaaatactct gaaatgacaa gtaatcattt cagagcacgc
     5821 aaaaagcaca aatatacttt tatcttcttt catccagact atactgtcgg ctttggaatt
     5881 tcaccaaatc atgcctttcg gctcgtgggc tataccaccg gtagggaatt acaccctgcc
     5941 ctgaagatag ttattcaatt acagatgatt atagtactta attttgaata tgtcaacaga
     6001 taaataccga ttgtttttga tatactgtat ttgtgataat cgattctcgc tcctcggata
     6061 aagaaaatat gatatactag ataaacgaaa taagagagaa ggaatactat gtacgcatat
     6121 ttaaaaggaa tcattaccaa aattactgcc aaatacattg ttcttgaaac caatggtatt
     6181 ggttatatcc tgcatgtggc caatccttat gcctattcag gtcaggttaa tcaggaggct
     6241 cagatttatg tgcatcaggt tgtgcgtgag gacgcccatt tgctttatgg atttcgctca
     6301 gaggatgaga aaaagctctt tcttagtctg atttcggtct ctgggattgg tcctgtatca
     6361 gctcttgcta ttatcgctgc tgatgacaat gctggcttgg ttcaagccat tgaaaccaag
     6421 aacatcacct acttgaccaa gttccctaaa attggcaaga aaacagccca gcagatggtg
     6481 ctggacttgg aaggcaaggt agtagttgca ggagatggcc ttcctgccaa ggtcgcagtg
     6541 caagcaagtg ctgaaaacca agaattggaa gaagctatgg aagccatgtt ggctctgggc
     6601 tacaaggcaa cagagctcaa gaaaatcaag aaattctttg aaggaacgac agatacagct
     6661 gagaactata tcaagtcggc ccttaaaatg ttggtcaaat aggagcagag aatgacaaaa
     6721 cgttgttcgt gggtcaagat gaccaaccca ctctacatcg cctatcatga tgaggagtgg
     6781 ggccagcccc tccatgatga ccaagcgttg tttgagttat tgtgtatgga aacctatcag
     6841 gcaggcctgt cttgggaaac ggtactcaac aaacgccaag ctttccgaga agcctttcat
     6901 agctatcaaa ttcactcagt cgcagagatg actgacactg aattggaagc catgctggag
     6961 aatccagcta tcattcgaaa tagagccaag atttttgcta cacgcgctaa cgcccaagct
     7021 tttctacagt tacaggcaga gtacggctct tttgatgcct atctttggtc ttttgttgag
     7081 gggaaaactg tcgttaacga tgttcctgat tatcgccaag ccccagctaa aacaccctta
     7141 tctgagaaat tagccaaaga tctcaaaaaa cgagacttca agttcacagg cccagtcgcc
     7201 gtattgtctt ttctacaggc tgcagggcta gttgatgacc acgagaatga ttgtgagtgg
     7261 aaaggtctta aatgatgtct aacaaaaata agggaattct gatttttgcg attctctata
     7321 cagtcctctt tgtgtttgat ggcgttaaat tgctggcttc tttaatgcca tctgccattg
     7381 caaattatct tgtttatgta gttttagctc tatatggctc cttcttattc aaggatagat
     7441 tgatccaaca atggaaggag attagaaaga ctaaaagaaa attcttcttt ggcgtcttaa
     7501 caggatggct ctttctcatt ctgatgactg ttgtctttga atttgtatca gagatgttga
     7561 agcagtttgt ggggctagtc ggacaaggtc taaatcagtc taatattcaa agcacttttc
     7621 aagagcaacc actactgata gctgtttttg cttgtgtcat tggacctctg gtagaagaat
     7681 tatttttccg tcaggtctta ttgcattact tgcaggaacg gttgccaggt ttactaagca
     7741 ttattctggt aggacttgtt tttgctctga ctcatatgca cagtttggct ctatcagagt
     7801 ggattggtgc agttggttac ttaggtggag gccttgcctt ttctattatt tatgtgaaag
     7861 aaaaagagaa tatctactat cccctacttg ttcacatgtt aagcaacagc ctctccttaa
     7921 tcattttagc tatcagtata gtaaaatg
//
LOCUS       DAVBCN010000080         7479 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVBCN010000080 DAVBCN010000000
VERSION     DAVBCN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7479
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.080"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            64..933
                     /locus_tag="VQ497_001769"
     CDS             64..933
                     /locus_tag="VQ497_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET2518049.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            937..1893
                     /locus_tag="VQ497_001770"
     CDS             937..1893
                     /locus_tag="VQ497_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET2518050.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKLPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            1893..2657
                     /locus_tag="VQ497_001771"
     CDS             1893..2657
                     /locus_tag="VQ497_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2518051.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            2657..3367
                     /locus_tag="VQ497_001772"
     CDS             2657..3367
                     /locus_tag="VQ497_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2518052.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKIPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            3832..4488
                     /locus_tag="VQ497_001773"
     CDS             3832..4488
                     /locus_tag="VQ497_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2518053.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDESIDLPALREKFEANGIQVEEIARTSAKVL
                     "
     gene            4596..5691
                     /gene="prfB"
                     /locus_tag="VQ497_001774"
     CDS             join(4596..4667,4669..5691)
                     /gene="prfB"
                     /locus_tag="VQ497_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET2518054.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            5709..6401
                     /gene="ftsE"
                     /locus_tag="VQ497_001775"
     CDS             5709..6401
                     /gene="ftsE"
                     /locus_tag="VQ497_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HET2518055.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            6394..7320
                     /locus_tag="VQ497_001776"
     CDS             6394..7320
                     /locus_tag="VQ497_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2518056.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
BASE COUNT         2210 a         1292 c         1682 g         2295 t
ORIGIN      
        1 tataagaacc tatcaaaaag tgagggaaaa ccctcggaat tataaataga aagagtgaat
       61 cttatgctcc aacaactcgt aaatggtttg attctaggta gtgtttacgc gctgttagcc
      121 ctaggatata ccatggttta cggaattatc aagctcatca acttcgccca tggtgatatt
      181 tatatgatgg gagcctttat cggttatttc ttgatcaatt ctttccaaat gaatttcttt
      241 gtagcgctta ttgtagctat gctagcgaca gctattcttg gtgtcgtgat tgagtttctt
      301 gcttaccgac ctttgcgcca ctctactcgt attgctgttt tgattacggc tattggggtt
      361 tctttcctat tggagtatgg aatggtctat ctggttggtg ccaatacccg tgccttccct
      421 caagcgattc aaacagttcg atatgatttg ggaccaatta gcttaacaaa tgtgcagtta
      481 atgattttgg ccatttcctt gattttgatg attttgttac aagtcattgt ccaaaagact
      541 aagatgggga aagccatgcg tgcagtatca gtagatagcg acgcggcgca attgatgggg
      601 atcaatgtaa accgtacgat tagctttacc ttcgctttgg gttctgctct tgcgggtgcg
      661 gctggtgttc tgattgctct ttattataac tctcttgagc ctttgatggg ggttactcca
      721 ggtcttaaat ctttcgttgc cgcagtactt ggtggtatcg gaattattcc tggtgcggct
      781 cttggtggct ttgtgattgg tctattggaa acctttgcga ctgcctttgg gatgtcagat
      841 ttccgtgatg ccattgttta tggaatcttg ttgttgatct tgattgtccg cccagctggt
      901 atccttggta agaatgtgaa agagaaggtg taaacgatga aggaaaattt aaaagttaat
      961 attctatggt tactcctttt gttagctggc tatagcttga ttagtgtact ggtttcagtc
     1021 ggagtactta atctattcta tgtacagatt ttacaacaaa ttggaattaa tattattttg
     1081 gctgttggtc tcaacttaat cgttggtttt tcaggacaat tttcacttgg tcatgctggt
     1141 ttcatggcga ttggtgccta tgcagcagct attattggtt ctaaattacc aacctacggt
     1201 gccttctttg gagctatgct tgtaggggct ttgctttcag gagcagttgc cttacttgtc
     1261 ggcattccaa ccttgcgctt gaagggggac tatcttgcgg tagcaactct aggtgtttct
     1321 gaaattatcc gtatctttat catcaatggt ggaagtctta caaatggtgc ggcaggtatc
     1381 ttagggattc ctaactttac aacttggcaa atggtttact tctttgtcgt gattacaacc
     1441 attgcaacct tgaacttctt gcgtagccca attggccgtt caaccctctc tgttcgtgaa
     1501 gatgaaatcg ctgctgagtc agttggggtt aatacgacta aaattaaaat catcgctttt
     1561 gtctttggtg ccattactgc aagtattgct gggtcacttc aggcaggatt tatcgggtct
     1621 gttgtaccga aagattacac cttcattaac tcaatcaacg ttttgattat tgttgtattt
     1681 ggtggactcg gttccattac aggtgcgatt gtttcggcta ttgttctggg aattttgaat
     1741 atgcttctcc aagatgttgc tagtgtgcgt atgattattt acgctttagc cttggtattg
     1801 gtaatgattt tcagaccagg tggactcctt ggaacgtggg aactgagcct atcacgtttc
     1861 tttaaaaaat ctaagaagga ggaacaaaac taatggcatt acttgaagta aaacagttaa
     1921 ccaaacattt tggtggtcta acagctgttg gagatgtgac tcttgaattg aacgaagggg
     1981 aactggttgg attaatcggt ccaaacggag ctgggaaaac cacccttttc aaccttttga
     2041 ccggtgttta tgaaccaagc gagggaacag taaccctaga tggtcacctt ttgaatggga
     2101 aatcacctta taagattgcc tctttgggac ttggacgtac tttccaaaat atccgtctct
     2161 ttaaagattt aacagtttta gataatgttt tgattgcttt tggaaaccat cacaaacagc
     2221 atgtttttac tagtttctta cgcttaccag ctttttacaa gagtgaaaaa gaattaaagg
     2281 ctaaagcttt ggaattgttg aaaatctttg atttagatgg tgatgcagag actcttgcta
     2341 aaaatctttc ctacggacaa caacgtcgtt tggaaattgt tcgtgccctt gctacggaac
     2401 ctaaaattct cttcttagat gaaccagcag caggtatgaa cccacaggaa acagccgaat
     2461 tgactgagtt aattcgtcgt atcaaagatg aatttaagat tacaatcatg ttgattgaac
     2521 acgatatgaa tctggtcatg gaagtaacag aacgtatcta cgtacttgaa tatggccgtt
     2581 taattgctca aggaactcca gacgaaatta agaccaataa acgcgttatc gaagcttatc
     2641 taggaggtga agcctaatgt ctatgttaaa agttgaaaat ctttctgtgc attacggtat
     2701 gatccaagca gtccgtgatg taagctttga agttaatgaa ggagaagttg tttcccttat
     2761 cggtgccaac ggtgcaggta agacaactat tcttcgcacc ttgtcaggtt tggttcgacc
     2821 aagttcagga aagattgaat ttttaggtca agaaatccaa aaaataccag ctcagaaaat
     2881 cgtggcaggt ggtctttcac aagttccaga aggacgccac gtctttcctg gcttgactgt
     2941 tatggaaaat cttgaaatgg gagctttctt aaagaaaaat cgtgaagaaa atcaagctaa
     3001 cttgaagaag gttttctcac gctttcctcg tcttgaagaa cgtaagaacc aagatgcagc
     3061 tactctttca ggaggggaac aacaaatgct tgccatggga cgcgctctta tgtcaacacc
     3121 aaaacttctt cttttagatg aaccatcaat gggacttgcc ccaatcttca tccaagagat
     3181 ttttgatatc attcaagata ttcagaagca aggaacaacc gtcctcttga ttgaacaaaa
     3241 tgccaataaa gcacttgcaa tctctgaccg aggatatgta ttggaaacag gaaaaatcgt
     3301 cctatcagga acaggaaaag aactcgcttc atcagaagaa gtcagaaaag catatctagg
     3361 tggctaaaac aatccagtgg attgttttag tcggcagatg aagattacga agtaatcatc
     3421 aatatagtcc gggggccctt tttagtcggt agattgagat tgcgaagcaa tcatcaaatc
     3481 cagaggattg ttttagccgg cagataaaga ttacgaagta atcatcaata tagtccgggg
     3541 gcccttttta gtcggtggat gaagattgcg aagcaatcat caaatccaga ggattgtttt
     3601 agccggtaga ttgagattgc aaacaaatct gcatctacat tgaaagctta atttctaata
     3661 attgaaaaaa tcgaatgaaa agtttcttac cttcattcac agagctcgat tttagagctc
     3721 tttttgctag cttattcata cttttctaaa ttttgaaaaa gaaatgtaag cgtttgatag
     3781 atttgcaaaa agattgtata atagggataa gaatagaaaa ggagaagtct catggcagtt
     3841 aaagatttta tgacccgcaa ggtagtttat attagtccag atacaacagt atctcatgca
     3901 gcagatttga tgagagagca aggtttgcac cgtctgcctg ttatcgaaaa tgatcaattg
     3961 gttggtttgg tgactgaggg aaccattgca caagcaagtc catctaaagc aacaagtctt
     4021 tctatctatg agatgaatta tcttctgaat aagacaaaag taaaagatgt catgattcgc
     4081 gatgttgtca ctgtctcagg ctatgctagt ctagaagatg caacttatct gatgttgaaa
     4141 aataagattg gtattctccc tgtcgtagat aaccatcaag tatacggagt tattactgac
     4201 cgtgacgttt tccaagcctt tcttgaaatt gcaggttatg gcgaagaagg gattcgtgta
     4261 cgctttgtta cagaagatga agttggtgtt cttggaaaaa ttgtttcttt gattgtagaa
     4321 gaaaatttga atatctccca tacagtcaat attccgcgta aggatggtaa ggtgattatc
     4381 gaagtgcaaa tcgatgaatc aattgattta ccagccttga gagaaaaatt tgaagcaaat
     4441 ggtattcaag tggaagaaat cgctcgcact tcagcaaaag tcttgtaaga agggaagtcc
     4501 aaaggcttct tttttcatga aaaggggatt agagcaaaag atggaaagaa atgataaaat
     4561 atgctataat gaaataatgt aaaaaaggag tatttatgga catttcagta attcgtcaga
     4621 aaattgacgc aaatcgtgaa aaattagctt ctttcagggg gtctctttga cctcgaaggg
     4681 ctagaggaag agattgccat cttggaaaac aagatgacag aacctgattt ttggaacgat
     4741 aatattgcgg cccaaaaaac gtcgcaagaa ttgaatgaat taaaaaacac ttacaatacc
     4801 ttccataaga tggaagagtt gcaggatgaa gtcgaaattt tattggattt tttggctgaa
     4861 gacgagtcag tgcatgatga actggtagcg cagttagccg aacttgataa gataatgacc
     4921 agctacgaga tgactctact cttgtcagaa ccttatgacc acaacaatgc catcttggaa
     4981 atccatccag gttctggtgg tactgaggcg caggactggg gtgatatgtt gcttcgtatg
     5041 tatactcgtt atggtaatgc taaaggcttt aaagtggaag tgttggatta ccaagcaggt
     5101 gatgaggctg gtattaagtc ggtaacttta tcatttgaag ggcctaatgc ctatggtctc
     5161 ctcaagtcag aaatgggtgt tcaccgctta gtgcgaatct caccatttga ctctgccaaa
     5221 cgtcgccata cctctttcac atctgtagaa gtgatgccag aattggatga tactattgaa
     5281 gtggaaatcc gtgaagatga tatcaagatg gataccttcc gttcaggtgg tgccggtgga
     5341 caaaacgtca ataaggtttc aacaggtgta cgtttaaccc acattccaac tggaattgtt
     5401 gtccaatcaa cagtggatcg tacccagtat ggaaatagag atcgtgccat gaagatgttg
     5461 caggctaagc tctatcaaat ggagcaagat aagaaggctg cggaggtaga ttctctcaaa
     5521 ggtgagaaaa aggagatcac ttggggaagc caaatccgtt cttatgtctt cacgccttat
     5581 actatggtaa aagatcaccg aactagcttt gaggttgctc aggtagataa ggttatggat
     5641 ggggacctag atggttttat agatgcttat ctcaagtggc gaattagcta agatagaaag
     5701 gaactcacat gtcaattatt gaaatgagag atgtcgttaa aaaatacgac aacggaacga
     5761 ctgctctacg cggtgtttcg gtcagcgttc aaccggggga atttgcttac atcgtaggac
     5821 cttcaggagc agggaagtca acttttattc gttctctgta tcgtgaagta aaaatcgata
     5881 aaggaagtct atcagttgct ggttttaatc tggttaagat caaaaagaaa gatgtcccgc
     5941 ttctacgtcg tagtgttggg gttgtcttcc aagattataa attgttacca aagaaaactg
     6001 tctatgaaaa tattgcttac gctatggaag taatcgggga aaatcgccgt aatatcaaaa
     6061 gacgagtgat ggaagttttg gacttggttg gattgaagca taaggttcgt tctttcccaa
     6121 atgaactctc aggtggggag cagcagcgaa ttgcgattgc gcgtgcaatt gtaaataatc
     6181 ccaaagtatt gatagctgat gagccaacag gaaatctgga tccggataat tcatgggaaa
     6241 ttatgaatct cttggaacgg attaacctac aagggacaac tattttgatg gcgactcata
     6301 atagccagat tgtaaatacc ttgcgccacc gtgtcattgc cattgaaaat ggccgtgtcg
     6361 ttcgtgacga atcaaaagga gagtatggat acgatgatta gtagattttt tcgccattta
     6421 tttgaagcct taaaaagttt gaaacgaaat ggttggatga cagtagctgc tgtcagttca
     6481 gtcatgatta ctttgacctt ggtggcaata tttgcatctg ttattttcaa tacagcgaaa
     6541 ctagctacag atattgaaaa taatgtccgt gtagtagttt atatccgaaa ggatgtggaa
     6601 gataatagtc agacaattga aaaagaaggt caaactgtta caaataatga ctaccacaag
     6661 gtatatgatt ctttgaagaa catgtctacg gttaaaagtg ttaccttttc aagtaaagaa
     6721 gaacaatatg aaaaattaac cgagataatg ggagataact ggaaaatctt tgaaggagat
     6781 gccaatcctc tctatgatgc ctatattgta gaggcaaaca ctccaaatga tgtaaaaact
     6841 atagccgaag atgctaaaaa aattgaaggt gtctctgagg ttcaagatgg cggtgccaat
     6901 acagaaagac tcttcaagtt agcttcattt atccgtgttt ggggactagg gattgctgct
     6961 ttgttaattt ttatcgcagt tttcttgatt tcaaatacca ttcgtattac cattatttcc
     7021 cgcagtcgcg aaattcaaat catgcgcttg gttggagcta aaaacagtta tatccgtgga
     7081 ccgttcttgt tagaaggagc ctttatcggt ttattgggag ctatcgcacc atctgttttg
     7141 gtctttattg tttatcaaat tgtttaccaa tctgtcaaca aatcgttggt agggcaaaat
     7201 ctatccatga ttagtccaga tttatttagt ccgttgatga ttgccctact atttgtgatt
     7261 ggggttttca ttggttcatt gggatcagga atatccatgc gccgattctt gaagatttag
     7321 gtaaaatagc tgcttttatg aggagattgt aaaatctcct tttttgctac aagagttttt
     7381 gaaaagagat gcgtagaaga aaagagcttc caaagaagtc ccccagagaa gacttcttga
     7441 tggctaattt gaacttgaaa tttggcgcac tagaaattt
//
LOCUS       DAVBCN010000081         7452 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVBCN010000081 DAVBCN010000000
VERSION     DAVBCN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7452
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.081"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(24..857)
                     /locus_tag="VQ497_001777"
     CDS             complement(24..857)
                     /locus_tag="VQ497_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2518057.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKSTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIIAGISNGAV
                     K"
     gene            complement(871..1737)
                     /locus_tag="VQ497_001778"
     CDS             complement(871..1737)
                     /locus_tag="VQ497_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2518058.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            complement(1751..3010)
                     /locus_tag="VQ497_001779"
     CDS             complement(1751..3010)
                     /locus_tag="VQ497_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2518059.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADDTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRSE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            complement(3102..5264)
                     /locus_tag="VQ497_001780"
     CDS             complement(3102..5264)
                     /locus_tag="VQ497_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HET2518060.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATITSYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIATLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIILSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYFYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            5372..6232
                     /locus_tag="VQ497_001781"
     CDS             5372..6232
                     /locus_tag="VQ497_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET2518061.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFYYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            6229..7164
                     /locus_tag="VQ497_001782"
     CDS             6229..7164
                     /locus_tag="VQ497_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HET2518062.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
BASE COUNT         2382 a         1544 c         1337 g         2189 t
ORIGIN      
        1 tgctctttga ttttcattga gtattacttc actgccccgt tgcttattcc tgcgatgata
       61 tggcgttgga agaagagata gacaatggtg atactgataa tgccgaccac gtaagaggca
      121 aagcttggtc cgtagtcgtt gaaatattgg cctgcgtagt tgtattggaa caaaggcaga
      181 gtccacattt tggaatcccg gttcaagaca aggagtggca acatgaagtc attccagaac
      241 caaagggcat tgatgatcat ggttgtcgca tgcatcggtt tcatcattgg gaagatgatg
      301 cggaaatagg ttgtaaattg attagcccca tcgatctctg ctgcttcatc cagactttct
      361 ggaatcgaga ttttgatata gccaacatag agaaagaggg tctgtggaat cgcataggtc
      421 aagtagagca agatcaaacc aaaggtatta gccaaaccga gtttactcat cataaccgta
      481 atcggaatca tgatgacttg gaaaggtacg aagattccga ggattaagag ggtatacatg
      541 atggtaaagg cttttctttt actcatattg cgagcgatgg agtaggctgc catagggata
      601 aagatcatta ctgcaagtaa agacaagaca gtgatgacga cagagttcca ataatagcct
      661 ccaatcccat cagctaagag acggctaaag ttgtcccatg tgaagttggt tggaaagcca
      721 aagaaattat ctacaatatc cttagtggat ttgaaggaac taaagagggt agcaaggagc
      781 ggcactaaaa tcagaaccga tcctagaatc aatagaatgt atttgccaat cagggctttt
      841 ctttcatctt gtttcatcat gcttctcctc ttaaatttca aatttcttag atactctcaa
      901 ttggatgatc gaaatcacta caattaagaa gaacaagatt acggcaatgg cattggcata
      961 accgaattgg ttgtttttaa aggcatagtt ataaaccaag agcccaagtg aggttgtggc
     1021 attgtttgga ccaccaccgg tcatggcaaa gacttggtca aaggcagtca gcccaccttt
     1081 tagggctagg ataaagacta tagagacact tggtagcaag taaggcaatt caatgttcca
     1141 gaaaacttgc ttgctagtcg caccatcaat ccttgctgcc tctgtaatct cagttggaat
     1201 agattgcaaa ccagctagga agatgatgat gggcatagcc accccttgcc aaagaaggac
     1261 aaagacagcc gcaaagattg ctccccactt agtccctaaa agactggttt ggagaaattc
     1321 aatatgaagg gcattcccaa tcgctggaag accgtagttg aagacttgct tgaagatcaa
     1381 agccactgtc aaaccagata aaacagctgg gaagaagaac caagcacgga agaaggtttg
     1441 gcctttgatt ttagagttca agacacgcgc aatgaagatc ccgagtgcaa tctcaccaac
     1501 caccatggca atcgtaatga ttgcggtaaa gccaatcgca ttcatgaatt ttggatccat
     1561 gaagaggagc ttaaagttgt ttaagccgac aaatttgtag ttgtaagtca atcctgtcca
     1621 gttggtaaaa ctgtaaaagg ctccttgaaa catcggcaca tagaagaaaa ttgcttgtaa
     1681 caagaggggg atgaccacaa aagcccatgc ccaatatttt tgtaatactt ttttcatagt
     1741 ctctctactc ctaatccaca tccgctttca tcgggttaaa gaaggcattc aaatcattga
     1801 ccatgccttg tttatcaccg gtcaagacat agttcatggt caaggtatgg aagtctgctt
     1861 cactggtcca gtattgttgc aaccagacca agtgacgatc cgtaaaggca tattcggtca
     1921 taccagcaag cggtgaatct tctcctgctt gtttgacccc ttcgatcgct gttggagatc
     1981 cgtccacatc gtagtatttt tgcatgactt ctgaacgggt catatattcc acaaaggcat
     2041 tggcttcttt tggatgtttg gtggtggctg agatagacca tgccaagtct cccgcaccaa
     2101 cggttaagct ttgtcctttt tcttttcctg gaatcatgaa ggttccaatc ttaaagttcg
     2161 gtttttgttc attaatcgct gtgatcgccc aagacccatt tggtgtcatg aggacatccc
     2221 cacgtgcgaa ggctccaata acatcggtat agccagcacc ttcccagttc ttttgcttag
     2281 agccattaat gcgaaggatg tccatgacct tgatatcatc tttcataatc ggatccgaca
     2341 atttaatagc atttggttga gaataacgaa ggtattgatt tgcttctttt cctccacctg
     2401 ttgctgtcgc aaaggctaat tgattgtaac cattgagtgt ccaagcatct gcacctgcaa
     2461 ttccaaatgg tgtttgtcct ttagcaacga tatctttgac taactgttca aattcatccc
     2521 aggtttcagg aaccttcaag cccagttctt cgaatttatc tttgttgtag taaattccat
     2581 aagcattagc tgtaaaagga acgttgtaaa ctttttcgtt tacagcatat ttttcagcgt
     2641 agccattttt cacgcgtttc aggtagtctt tgttactcaa atcttcaaag acacctgctt
     2701 ttgcccattc ttgcagttcg atggactgtg ggtaaatatt gaccacatca ggcacatctc
     2761 ctgcgagaac gcgtgtcttc aatacttcac cagcatttgg tacattgacg actttgacct
     2821 ttatcttagg gttttccttc tcaaaatcac gagtgatttc ttccaaggtt ttggtcattt
     2881 cttttttctg gttgaaatac tcgatggtca ctgtgtcatc cgcagattta ccatagttgg
     2941 agcaagcacc gagcccaaac aaagctaaac ctgtagttgc aagaagtccg atttttttat
     3001 accattccat tagaaagcct cctttataaa tttatacacc cttatcaaac tgcacttcaa
     3061 aagttagata gaataaatct aacttttggg gtcagtacat atcatagttt tctaaaaata
     3121 tactgtctac tcaaaaaatc tccttgggat aagataacag ttaagcccgc atacattagt
     3181 tctgcacctg agtaaacttc gccattttcc tgtaatttat atagtccctc ttcatccaaa
     3241 tcttttaatt ttaaagttgt ttccatggtc tctacaacag ataaaacgcg aacgtaggtt
     3301 acaatcgttt gatttccgta attaaattgt acagctgctt cattggatgc agtatcagga
     3361 ttaattagtc tatactgctg tcctaactga actactggtc gtaattcttt atacaagttc
     3421 acctgattag caatcgtagc tttctcttca tctgataaat ttgtcaaatc aagctcatag
     3481 cccaaatttc ccatcattgc tacatggcca cgtgtttcta atggtgtcat tcgtcccatc
     3541 tgatgattcg gtactgctga cacatgagcc cccatagaaa tggttggata gaaataggat
     3601 gaaccgtatt gaattggtaa acgtgcaatg gcatcagtat tatcactagc ccagacttgt
     3661 gggaaatagc gtatcatacc aagatcattt cgtccaccac caccagagca ggactcaaag
     3721 agaatatggc tgtgcttctc tgtcagataa gaaacgagtt cataaagccc cagcatgtac
     3781 tgatgagatt gcatctgtgt ctctagataa gttaatccat tccctagctt agtgatattg
     3841 cggttcatat cccatttaat gtaatcaatc tcatgataag ataggagttg atctaagaca
     3901 cttttcaagt attctactac ctgaggattg gcaagattaa gtactaattg attccgagaa
     3961 taagtatgct catagtcagg aacctgaata gcccagtcag ggtgttgacg atacaaatca
     4021 ctatctacag aaatcatttc gggttctaac caaagtccaa actgcaaacc tctttcatgg
     4081 atagctgaaa tcagactttc tagacttcca cccagttttt cctcattaac aacccaatca
     4141 cctaaagcac gattatcatc aaaacgattg ccaaaccaac catcatctaa tacaaaaagt
     4201 tcaatgccaa ctttcttagc ttcatctgct aactctaaca gtttttctct ctgaaagtca
     4261 aagtaagtag cttcccagtt attgattaga attggacgtt cttttttaga aaattcactt
     4321 agaataatgt gcttcagtac aaaattctga ctttcatgac taataccagt taatccctga
     4381 tctgaatgag tcactaaagc taccggtgtt tcaaagtatt cctcaggggc taacttccaa
     4441 gaaaagtttt ctggattaat gccaatagcc acccgaactt cattcaattg atttttttga
     4501 acaaaagctt caaagttgcc actatacatt agttgaatag caaacacatt cccagcatcc
     4561 tctgtgactc cttgttcgca tagtagaaga gctggtgttt gagcatgacc agaagcacct
     4621 cggtttgaac taatcgaaaa gattccttgt tctacctgtt gacgtctaac agtcttttca
     4681 cgagcataag caccctgcag agttgctatt tcgtaatctg cagctggaaa atcagccata
     4741 aaagaaaaat ctttatggat gacaacttcc tgattactat tattatctaa tttactgtag
     4801 ctagtaatag tcgcatcatt attaaaagta gtataataca aagtcagact aagttgagcc
     4861 ttagaatctt ctaacattaa gacaagagtc tctgtatcgt ccatgctatg tggagaaggt
     4921 aagccctgtg gaccattctg accttttaaa atctttgctt ctacaaatcg aaagtctgtt
     4981 acttcagtta cactatgctg aacctgtata gttggtttcc taaaatctcc taagccatgt
     5041 tgtccaaaaa tctgtcgctg agtatctaaa ctaaaggttc gattagtagc cgttggattt
     5101 cctgaaaagg catggtctcg ttcataaaca ctattggaac ctttatagtt cttaatagtc
     5161 tttcctaaat gtttcaaaag taagtagcca tttcgatttt caataatcaa acttagattt
     5221 ttactctcaa cataaaatag attattctct atcctaactc ccatttactt cacctcatca
     5281 ctttattgat tatattttat cacctgaaat cgctttccaa aatagaaaaa tgtctcaaga
     5341 atatggtaaa atgttaggta ggaggtagca catgttagtt ttttcagaat accagactgg
     5401 aacaatcgac cttgccctaa gcttttatgg atatgaggaa tgcacaccta attactcttt
     5461 tggtccagcc attcgtgata catacgttct acattacatt actaaaggac aaggaaaatt
     5521 ttattacaag ggtaaaattg ttgatttaaa agaaggagat ttctttctat taaaaccaga
     5581 ggaactaacc ttttatcaag cagatagtaa agaaccttgg gcctactact ggttaggaat
     5641 cactggaggg aaatcccctg attattttgc tctttctcaa atttctgatc aatcctatct
     5701 catccaatct gaaacttgtc atacccagac tactgcaaaa ctcatctcag acattgtccg
     5761 cttcgctcag attacaaaat caagtgaatt agctcaactc catatcatgg gacaacttca
     5821 tgaactgatg tttcatctgg gaactattgc tcccaatcag aaaaaaaaga atatttcatc
     5881 aacccaccaa ctctatcttg aatgcaaacg attaattgat agccactatc ctcaatcact
     5941 tacaattcaa gatttagcaa aagaactatc cgttcacaga agctacttat caagcgtatt
     6001 caaagaattt aataccttat cacccaaaga atacctactc tacgttcgaa tgcaccgagc
     6061 tagacaactt ctcgaaaata cccaagagtc catcaaggta attgcatact cggtaggttt
     6121 ttcagatcca ctccattttt cgaaagctta taaacaatac tttaatcaga ctccaagtca
     6181 tacaagaaaa gaatactctc aataccaact agtgagaaag gcaacattat gaaatcctac
     6241 caagctgtct accaaatcct atctaaagaa accgactata tcagcggaga aaaaatcgca
     6301 gaaaaactat ccctaagccg aacagcaatt tggaaagcca tcaagcgact agaacaagaa
     6361 ggcattgaaa ttgatagtat caaaaataga ggatataaac tgatgaatgg tgaccttatt
     6421 cttccagaga ttctagaaga aaatcttcca attaaagtca gctttaaacc cgaaacaaaa
     6481 tcaacacaac tagatgcaaa agaagcaatt gatttaggcc atgaagcaaa taccctctat
     6541 ctagcttcct atcaaacagc aggccgaggc cgttttcaac gttccttcta ctcaccacaa
     6601 ggtggtattt atatgacact ccatcttaaa ccaaatctcc cctatgacaa attaccatcc
     6661 tacacactac ttgtagctgg agctgtctac aaagccatta agaacctaac tttaatagat
     6721 gtcgacataa aatgggtcaa tgatatctat ctaaacaatc ataaaattgg aggaatcctt
     6781 actgaagcaa tgacctctgt agaaactggc ttagtcacag atatcattat tggagtaggt
     6841 atcaatttca ctattaaaga cttccctcag gaattaaaag aaaaagctgc cagcttattt
     6901 aaagctacag ctcctataac aaggaatgaa ttgatcatag aaatctggcg tgctttcttc
     6961 gaaacaccag cagaagagct attataccta tacaaaaaac agtcattcat tctaggaaaa
     7021 gaagtcactt tcacactaga gcaaaaagac tacaagggac ttgctaaaga catctcagaa
     7081 aatggaaaac ttttagttca atgtgataac ggaaaagaaa tctggctaaa tagtggcgaa
     7141 atttctctca atagttggaa gtaaaataac acaattataa tataaacgat ataaaaataa
     7201 cttcagatta gtaattcaat taagttttac ggatctgaag ttttattgtc tctaaaaaca
     7261 aaaaagagag ttacaaactc tcattaaaac gggctctata atatttgtag tgggtaaatt
     7321 ccctatggat attatggagc ctattttgtg tagaaaaaaa gtcccatatg acctataatg
     7381 aaaagcgaca aaacaactca ttagaaagaa tcatatggaa caattacatt ttatcacaaa
     7441 attactagac at
//
LOCUS       DAVBCN010000082         7343 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVBCN010000082 DAVBCN010000000
VERSION     DAVBCN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7343
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.082"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(317..2008)
                     /locus_tag="VQ497_001783"
     CDS             complement(317..2008)
                     /locus_tag="VQ497_001783"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET2518063.1"
                     /translation="MNTKELIASELASIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            2144..2590
                     /gene="argR"
                     /locus_tag="VQ497_001784"
     CDS             2144..2590
                     /gene="argR"
                     /locus_tag="VQ497_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2518064.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            2641..5175
                     /gene="mutS"
                     /locus_tag="VQ497_001785"
     CDS             2641..5175
                     /gene="mutS"
                     /locus_tag="VQ497_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET2518065.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKLDSQNNFLVAIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSCQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPALAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLITGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTENPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            5322..7016
                     /gene="patA"
                     /locus_tag="VQ497_001786"
     CDS             5322..7016
                     /gene="patA"
                     /locus_tag="VQ497_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HET2518066.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDVPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
BASE COUNT         2002 a         1413 c         1735 g         2193 t
ORIGIN      
        1 tttggttagg tgaattagtg aagcgtttag gcaagtgtct ctggttacga cgtcatggac
       61 tctaaatcga ttatatttag gggtcatgac tagtgaagca gttagctagt tcgcatataa
      121 gcggctagcg tctaacaatt aggaacttta gttccaataa ctttaagatt acgacgtttt
      181 aggacataaa tcgatcatat ttatgtccta aaactagtga agtatgtcct aaaactagtg
      241 aagcgcctag ccaaagtccg aataggattt ggcgttagtt acttagattg ctttgcaatc
      301 aagtaacttt ggcgatttac atcttctctg gcgcttctac tccaagcaag cgaagggctt
      361 ctttgagaac gactgcggtt gcgtagctga gggctagacg gctgtcgcgt tctgggcttt
      421 catccaagat acgtgtatgt gcatagtatt tgttaaagga ttgagccagg ctaattgcaa
      481 atttagcaat gatagaaggt tcaaagttat ctgccgcacg gttgataata cgtgggaagt
      541 cttgaatgag tttaatgatt tcccagcttt cagtatcatt caagctatag ttgccagctg
      601 tttctggttt gaaatcggct ttgcgtaaga tagattggat acgagcgtag gcatattgaa
      661 cgtaaggtcc agtttcaccc tcgaaggata ccatagcctc taggtcgaag tcgtatccat
      721 ttgtacggtc ggttttgagg tcatagaatt taatggctcc aaccccaaca gcatgtgcta
      781 cttggtcttt gttttctagt tcaggatttt tagcctcgat ttggaccttg gcacggctaa
      841 cagcctctgc aacagtaggc tctagcaaga tgacattccc tttacgagta gagagtttct
      901 tcccttcttt tgtaaccaaa ccaaaaggaa cgtgagtaat gtcgtcactc cagtcgtagc
      961 ccatctcttg caagacagct ttgagctgtt taaagtgggc agattgttct tgaccaacga
     1021 catagataga tttagcaaat tggtattcgt ttttacggta gagggctgca gccaagtcac
     1081 gtgtgatata gagagttgca ccatcagact tcttgatgag ggctggatgt tcaattccgt
     1141 atttctcaag attcacaact tgggcacctt ctgattcaag aagtagtcct ttttcagaaa
     1201 gaatgtctac aactgcatcc atcttatcat tgtagaaggc ttctccgtta tagctgtcaa
     1261 attcaacctt caattcattg taaaggcggt taaattccac taaactttca tcgcggaacc
     1321 attgccaaag agcgagagct tcctcatctc cattttcaag tttacggaac cattcgcgcg
     1381 cttcttcatc caagctaggg tcattttcag cttcagcgtt gatgcggaca tagagtttaa
     1441 ggagttcatc gattggatga gcttttacag cttcttcgtc gccccatttt ttgtaggcaa
     1501 caatcaacat cccaaattgt ttaccccagt ctcccaaatg gttgaccttg accgtttgat
     1561 aaccgatttt ttggaaaata tgtgacaagc tatctccgat aacagttgaa cgcaggtggc
     1621 caatagaaaa tggtttagcg atattcggac tagacatgtc gataacaaca ttttcttgtt
     1681 taccaatatt ttggtcagca tagtgttctt tttcagtggt aacagcttgc aatacttgag
     1741 cagaaatggc agatttatca aggaaaaagt taacgtaagg tcctgttgcg acaacttttt
     1801 caaaggcttg gctgttcatt ttttcagcca gttcagccgc aatcatttgt ggtgctttac
     1861 gttcgacttt ggcaagagaa aaagcaggga aagcgatgtc acccatttct gagtttttag
     1921 gggtttccag taactttaaa atagcctctt ggtccaggct atcaatgatg ctagccaatt
     1981 cgctagcaat caattctttt gtattcatta agagctcctt tttggacttt tctactattt
     2041 tatcacaatt ttaaagaaag aagaaaaaat ttttgaaatc tcctgttttt ttggtataat
     2101 atggttataa atatagttat aaatatgcac gcaagaggat tttatgagaa aaagagatcg
     2161 tcatcagtta ataaaaaaaa tgattactga ggagaaatta agtacacaaa aagaaattca
     2221 agatcggttg gaggcgcaca atgtttgtgt gacgcagaca accttgtctc gtgatttgcg
     2281 cgaaatcggc ttgaccaagg tcaagaaaaa tgatatggtg tattatgtac tagtaaatga
     2341 gacagaaaag attgatttgg tggaattttt gtctcatcat ttagaaggtg ttgcaagagc
     2401 agagtttacc ttggtgcttc ataccaaatt gggagaagcc tctgttttgg caaatattgt
     2461 agatgtaaat aaggatgaat ggattttagg aacagttgct ggtgccaata ccttattggt
     2521 tatttgtcga gatcagcacg ttgccaaact catggaagat cgtttgctag atttgatgaa
     2581 agataagtaa ggtcttggga gttgctctca agacttattt ttgaaaagga gagacagaaa
     2641 atggcgatag aaaagctatc acccggcatg caacagtatg tggatattaa aaagcaatat
     2701 ccagatgctt ttttgctctt tcggatgggt gatttttatg aattatttta tgaggatgcg
     2761 gtcaatgctg cgcagattct ggaaatttcc ttaacgagtc gcaacaagaa tgccgacaat
     2821 ccgatcccta tggcgggtgt tccctatcat tctgcccaac agtatatcga tgtcttgatt
     2881 gagcagggtt ataaggtggc tatcgcagag cagatggaag atcctaaaca agcagttggg
     2941 gttgttaaac gagaggttgt tcaggtcatt acgccaggga cagtggtcga tagcagtaag
     3001 ctggacagtc agaataattt tttggttgcc atagaccgcg aaggcaatca atttggccta
     3061 gcttatatgg atttggtgac gggtgacttt tatgtgacag gtcttttgga tttcacgctg
     3121 gtttgtgggg aaatccgtaa cctcaaggct cgagaagtgg tgttgggtta tgacttgtct
     3181 gaggaagaag aacaaatcct cagctgccag atgaatctgg tactctctta tgaaaaagaa
     3241 agctttgaag accttcattt attggatttg cgattggcaa cggtggagca aacggcatct
     3301 agtaagctgc tccagtatgt tcatcggact cagatgaggg aattgaacca cctcaaacct
     3361 gttatccgct acgaaattaa ggatttcttg cagatggatt atgcgaccaa ggctagtctg
     3421 gatttggttg agaatgctcg ctcaggtaag aaacaaggca gtcttttctg gcttttggat
     3481 gaaaccaaaa cggctatggg gatgcgtctc ttgcgttctt ggattcatcg ccccttgatt
     3541 gataaggaac gaatcgtcca acgtcaagaa gtagtgcagg tctttctcga ccatttcttt
     3601 gagcgtagtg acttgacaga cagtctcaag ggtgtttatg acattgagcg cttggctagt
     3661 cgtgtttctt ttggcaaaac caatccaaag gatctcttgc agttggcgac taccttgtct
     3721 agtgtgccac ggattcgtgc gattttagaa gggatggagc aacctgctct agcctatctc
     3781 atcgcacaac tggatgcaat ccctgagttg gagagtttga ttagcgcagc gattgctcct
     3841 gaagctcctc atgtgattac agatggggga attatccgga ctggatttga tgagacttta
     3901 gacaagtatc gttgcgttct cagagaaggg actagctgga ttgctgagat tgaggctaag
     3961 gagcgagaaa actctggtat cagcacgctc aagattgact acaataaaaa ggatggctac
     4021 tattttcatg tgaccaattc gcaactggga aatgtgccag cccacttttt ccgcaaggcg
     4081 acgctgaaaa actcagaacg ctttggaacc gaagaattag cccgtatcga gggagatatg
     4141 cttgaggcgc gtgagaagtc agccaacctc gaatacgaaa tatttatgcg cattcgtgaa
     4201 gaggtcggca agtacatcca gcgtttacaa gctttagccc aaggaattgc gacggttgat
     4261 gtcttacaga gtctggcggt tgtggctgaa acccagcatt tgattcgacc tgagtttggt
     4321 gacgattcac aaattgatat ccggaaaggg cgccatgctg tcgttgaaaa ggttatgggg
     4381 gctcagacct atattccaaa tacgattcag atggcagaag ataccagtat tcaactgatt
     4441 acagggccaa acatgagtgg gaagtctacc tatatgcgtc agttagccat gacggcggtt
     4501 atggcccagc tgggttccta tgttcctgct gaaagcgccc atttaccgat ttttgatgcg
     4561 atttttaccc gtatcggagc agcagatgac ttggtttcgg gtcagtcaac ctttatggtg
     4621 gagatgatgg aggctaataa tgccatttcg catgcgacca agaactctct cattctcttt
     4681 gatgaattgg gacgtggaac tgcaacttat gacgggatgg ctcttgctca gtccatcatc
     4741 gaatatatcc atgagcacat cggagctaag accctctttg cgacccacta ccatgagttg
     4801 actagtctgg agtctagttt acaacacttg gtcaatgtcc acgtggcaac tttggagcag
     4861 gatgggcagg tcaccttcct tcacaagatt gaaccgggac cagctgataa atcctacggt
     4921 atccatgttg ccaagattgc tggcttgcca gcagaccttt tagcaagggc ggataagatt
     4981 ttgactcagc tagagaatca aggaacagag aatcctcctc ccatgagaca aactagtgct
     5041 gtcactgaac agatttcact ctttgatagg gcagaagagc atcctatcct agcagaatta
     5101 gctaaactgg atgtgtataa tatgacacct atgcaggtta tgaatgtctt agtagagtta
     5161 aaacagaaac tataaaacca agactcacta gttaatctag ctgtagcaag gagacttctt
     5221 tgacaattct ccactttttt gctagaatga catcacacaa acagaatgaa aaggagctga
     5281 cgcattgtcg ctcccttttg tctatttttt aaggagaaag tatgctgatt cagaaaataa
     5341 aaacctacaa gtggcaggcc ctggcttcgc tcctgatgac aggcttgatg gttgctagtt
     5401 cacttctgca accgcgttat ctgcaggaag tcttaggcgc cctccttact gggaaatatg
     5461 aagctattta tagtatcggg gcttggttga ttggtgtggc cgtagtcggt ctagttgctg
     5521 gtggactcaa tgttgtcctc gcagcctata ttgcccaagg agtttcatcc gaccttcggg
     5581 aggatgcctt ccgtaaaatt caaacctttt cttatgctga tattgaacaa tttaatgcgg
     5641 gaaatctagt cgttcgaatg acaaatgata tcaaccagat tcagaacgtt gtcatgatga
     5701 ccttccagat tcttttcaga cttcccctct tgttcatcgg ttcgtttatc ctagcggttc
     5761 aaaccttacc ttctctgtgg tgggtgattg ttctcatggt agtcttgatt tttggtttga
     5821 ctgctgtcat gatgggaatg atggggcctc gttttgccaa gtttcaaacc cttcttgagc
     5881 gcatcaatgc cattgccaag gaaaatttac gtggcgttcg tgtggtcaag tcctttgtcc
     5941 aagaaaaaga gcaatttgct aagtttacag aggtctcaga cgagcttctt ggtcaaaacc
     6001 tttacattgg ttatgccttt tcagtagtgg aaccctttat gatgttggtt ggttacgggg
     6061 cggtcttcct ctctatttgg ctggtcgcgg gaatggttca gtcggatccg tctgttgttg
     6121 gttccatcgc ttcttttgtt aattacctaa gccagattat ctttaccatt gttatggttg
     6181 gatttttggg aaattctgtc agccgtgcca tgatttccat gcgtcgtatt cgagaaattc
     6241 ttgacgcaga gccagctatg accttcaagg atgtcccaga tgaagagttg gttggaagcc
     6301 ttagctttga aaatgtgacc tttacctatc caatggacaa ggaaccgatg ctgaaagatg
     6361 tgagctttac tattgaacct ggtcaaatgg ttggtgtagt tggagcgact ggtgcaggaa
     6421 agtcaacctt ggctcaattg attccacgtc tctttgatcc acaggaaggg gctatcaaaa
     6481 tcggaggtaa ggatattcga gaagtgagtg aaggaaccct gcgtaaaaca gtttccatcg
     6541 ttctccaacg tgccattctt tttagtggaa cgattgcaga taacttgaga caggggaagg
     6601 gaaatgctac tctatttgaa atggagcgcg cagccaatat tgcccaggct agtgaattca
     6661 ttcatcgtat ggagaaaacc tttgaaagtc cagttgaaga acggggaacc aatttctctg
     6721 gtggacaaaa acaaaggatg tcgattgcgc gtgggattgt cagcaatcca cgtattctga
     6781 tttttgatga ttcgacctca gccttggatg ccaaatcaga gcgcttggtg caagaagctt
     6841 tgaataagga cttgaagggg acgacaacca ttattattgc tcaaaaaatt agctcggttg
     6901 tccatgcaga caagatcttg gttctaaatc aaggacgatt gattggtcaa ggcacgcatg
     6961 cagacttggt tgccaacaat gccgtttacc gtgaaatcta tgaaacacag aaatgaaaga
     7021 caaactataa gaaaagtcaa tagttttatc taaactattt cttatttcaa tttgatgatt
     7081 tggcgatgat tttagagcac ggcaaaaagc ccttgaaaaa gtccattttt tcaaaggtaa
     7141 tcctgtgttg atttcagaaa ttacatcact ttttgttcgt caaatggcag ctctttttta
     7201 ggatataaaa cagggttcgg ataagttttt ttgcaaggtg gatgatggct acattgtaat
     7261 gttttccttg ttataactta gtcttaagat aggccttaaa agcaggtgaa aagtgagggc
     7321 atgctttggc agcttgtatg agt
//
LOCUS       DAVBCN010000083         7095 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVBCN010000083 DAVBCN010000000
VERSION     DAVBCN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7095
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.083"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            145..6831
                     /locus_tag="VQ497_001787"
     CDS             145..6831
                     /locus_tag="VQ497_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET2518067.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTVEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQTSNQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDVDVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGFKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVARVRTLIGKEPKMPKTVGFVYSDGSREKLPVTWSQ
                     VDVSQAGVVTVKGTANGREVEAHVEVLAIAKELPTVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRIEQYA
                     KEGVFTVNGRLEDTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQIRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLRFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGKERIFT
                     AINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSEASQTN
                     KAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
BASE COUNT         2336 a         1364 c         1581 g         1814 t
ORIGIN      
        1 catatctacg gcaaggcgac actgacgaag tttgaattgg attttcgcag agtgtaaatt
       61 gttgaaacta ggttgtcata ccatgtatac cacttgggct ttggtagtag ctaactgcac
      121 taaatataat ataaggaggg ttttatgggg aaaggccatt ggaatcggaa aagagtttat
      181 agcattcgta agtttgctgt gggagcttgc tcagtaatga ttgggacttg tgcagttcta
      241 ttaggaggaa atatagctgg agaatctgta gtttatgcgg atgaaacact tattactcat
      301 actgttgaga aacctaaaga ggaaaaaatg atagtagaag aaaaggctga taaagctttg
      361 gaaactaaaa atgtagttga aaggacagaa caaagtgaac ctagttcaac tgaggctatt
      421 gcatctgaga agaaagaaga tgaagccgta actccaaaag aggaaaaagt gtctgctaaa
      481 ccggaagaaa aagctccaag gatagaatca caaacttcaa atcaagaaaa accgctcaag
      541 gaagatgcta aagctgtaac aaatgaagaa gtgaatcaaa tgattgaaga caggaaagtg
      601 gattttaatc aaaattggta ttttaaactc aatgcaaatt ctaaggaagc cattaaacct
      661 gatgtagacg tatctacgtg gaaaaaatta gatttaccgt atgactggag tatctttaac
      721 gatttcgatc atgaatctcc tgcacaaaat gaaggtggac agctcaacgg tggggaagct
      781 tggtatcgca agactttcaa actagatgaa aaagacctca agaaaaatgt tcgccttact
      841 tttgatggcg tctacatgga ttctcaagtt tatgtcaatg gtcagttagt ggggcattat
      901 ccaaatggtt ataaccagtt ctcatatgat atcaccaaat accttcacaa agatggtcgt
      961 gagaatgtga ttgctgtcca tgcagtcaac aaacagccaa gtagccgttg gtattcagga
     1021 agtggtatct atcgtgatgt gactttacaa gtgacagata aggtgcatgt tgagaaaaat
     1081 gggacaacta ttttaacacc aaaacttgaa gaacaacaac atggcaaggt tgaaactcat
     1141 gtgaccagca aaatcgtcaa tacggacgac aaagaccatg aacttgtagc cgaatatcaa
     1201 atcgttgaac gaggtggtca tgctgtaaca ggcttagttc gtacagcgag tcgtacctta
     1261 aaagcacatg aatcaacaag cctagatgcg attttagaag ttgaaagacc aaaactctgg
     1321 accgttttaa atgacaaacc tgccttgtac gaattgatta cgcgtgttta ccgtgacggt
     1381 caattggttg atgctaagaa ggatttgttt ggttaccgtt actatcactg gactccaaat
     1441 gaaggtttct ctttgaatgg tgaacgtatt aaattccatg gagtatcctt gcaccacgac
     1501 catggggcgc ttggagcaga agaaaactat aaagcagaat atcgccgtct caaacaaatg
     1561 aaggagatgg gagttaactc catccgtaca acccacaacc ctgctagtga gcaaaccttg
     1621 caaatcgcag cagaactagg tttactcgtt caggaagagg cctttgatac ttggtatggt
     1681 ggcaagaaac cttatgacta tggacgtttc tttgaaaaag atgccactca cccagaagct
     1741 cgaaaaggtg aaaaatggtc tgattttgac ctacgtacca tggtcgaaag aggcaaaaac
     1801 aaccctgcta tcttcatgtg gtcaattggt aatgaaatag gtgaagctaa tggtgatgcc
     1861 cactctttag caactgttaa acgtttggtc aaggttatca aggatgttga taagactcgc
     1921 tatgttacca tgggagcaga taaattccgt ttcggtaatg gtagcggagg gcatgagaaa
     1981 attgctgatg aactcgatgc tgttggattt aactattctg aagataatta caaagccctt
     2041 agagctaagc atccaaaatg gttgatttac ggttcagaaa catcttcagc tacccgtaca
     2101 cgtggaagtt actatcgccc tgaacgtgaa ttgaaacata gcaatggacc tgagcgtaat
     2161 tatgaacagt ctgactatgg aaatgatcgt gtgggttggg ggaaaacagc aaccgcttca
     2221 tggacttttg accgtgacaa cgctggctat gctggacagt ttatctggac aggtacggac
     2281 tatattggtg aacctacacc atggcacaac caaaatcaaa ctcctgttaa gagctcttac
     2341 tttggtatcg tagatacagc cggcattcca aaacatgact tctatctcta ccaaagccaa
     2401 tgggtttctg ttaagaagaa accgatggta caccttcttc ctcactggaa ctgggaaaac
     2461 aaagaattag catccaaagt agctgactca gaaggtaaga ttccagttcg tgcttattcg
     2521 aatgcttcta gtgtagaatt attcttgaat ggaaaatctc ttggttttaa gactttcaat
     2581 aaaaaacaaa ccagcgatgg gcggacttac caagaaggtg caaatgctaa tgaactttat
     2641 cttgaatgga aagttgccta tcaaccgggt accttggaag caattgctcg tgatgaatct
     2701 ggcaaggaaa ttgctcgaga taagattacg actgctggta agccagcggc agttcgtctt
     2761 attaaggaag accatgcgat tgcagcagat ggaaaagact tgacttacat ctactatgaa
     2821 attgttgaca gccaggggaa tgtggttcca actgctaata atctggttcg cttccaattg
     2881 catggccaag gtcaactggt cggtgtagat aacggagaac aagccagccg tgaacgctat
     2941 aaggcgcaag cagatggttc ttggattcgt aaagcattta atggtaaagg tgttgccatt
     3001 gtcaaatcaa ctgaacaagc agggaaattc accctgactg cccactctga tctcttgaaa
     3061 tcgaaccaag tcactgtctt tactggtaaa aaagaaggac aagagaagac tgttttgggg
     3121 acagaagtag caagagttcg tacattaatt ggtaaagaac caaaaatgcc gaaaacagta
     3181 ggttttgtat acagcgatgg tagtcgtgaa aaacttccag taacttggtc tcaagtcgat
     3241 gttagtcaag caggagttgt gactgttaaa ggtacagcaa atggacgtga ggtagaggct
     3301 catgtcgagg ttctagcaat tgcgaaagaa ttaccaacag ttaaacgtat tgctccaaat
     3361 actgacttga attctgtaga caaatctgtt tcttatgttt tgactgatgg aagtgtacaa
     3421 gagtacgaag tagatagctg ggagattacg gaagtagata aagccaaact ttcagtagct
     3481 ggatcacgta ttcaaatgac tggtcagtta gctggagaaa ctattcatgc aacccttgtg
     3541 gtagaagaag gaaatgctgc agcacctgta gtgccaactg ttactgttgg aggtgaggca
     3601 gtaacaggtc ttactagtcg acaaccaatg caatatcgta ctctatctta tggtgcccaa
     3661 ctgccagaag tcacagcaag tgctgaaaat gctgatgtga cagttcttca agcaagcgca
     3721 gcaaacggca tgcgtgcgag catctttatt cagcctaaag atggtggtcc tcttcaaacc
     3781 tatgcaattc aattcctaga agaagcacca aaaattgctc acttgagttt gcaagtggaa
     3841 aaagctgaca gtctcaaaga agaccaaact gtcaaattgt cggttcgagc tcactatcaa
     3901 gatggaacgc aagctgtatt accagctgac aaagtaacct tctctacaag tggtgaaggg
     3961 gaagtcgcaa ttcgtaaagg aatgcttgag ttgcataagc caggagcagt cactctcaaa
     4021 gcggaatatg agggagctaa aggccaagtt gaactcacta tccaagccaa tactgagaag
     4081 aagattgcgc aatctatccg tcctgtaaat gtagtcacag atttgcatca aaaacctact
     4141 cttccaacaa cagtaacggt tgagtatgac aaaggtttcc ctaaagctca caaagtcact
     4201 tggcaagcta ttccgaaaga aaaactagac tcctatcaaa catttgaagt actaggtaaa
     4261 gttgaaggaa ttgaccttga agcgcgtgca aaagtctctg tagaaggtat cgtttcagtt
     4321 gaagaagtca gtgtgacaac tccaatcgca gaagcaccac aattaccaga aagcgttcgg
     4381 acatatgatt caaatggtca cgtttcatca gctaaggttg catgggatgc gattcgtata
     4441 gagcaatacg ctaaggaagg tgtctttaca gttaatggtc gcttagaaga tactcaatta
     4501 acaactaaac ttcatgttcg cgtatctgct caaactgagc aaggtgcaaa catttctgac
     4561 caatggaccg gttcagaatt gccacttgcc tttgcttcag attcaaatcc aagcgaccca
     4621 gtttcaaatg ttaatgacaa gctcatttcc tacaataacc aaccagccaa tcgttggaca
     4681 aactggaatc gtactaatcc agaagcttca gtcggtgttc tgtttggaga ttcaggtatc
     4741 ttgagcaaac gctccgttga taatctaagt gtcggattcc acgaagacca tggagttggt
     4801 gcaccgaagt cttatgtgat tgagtattat gttggtaaga ctgtcccaac agctcctaaa
     4861 aaccctagtt ttgttggtaa tgaggaccat gtctttaatg attctgccaa ctggaaacca
     4921 gttactaatc taaaagcccc tgctcaactc aaggctggag aaatgaacca ctttagcttt
     4981 gataaagttg aaacctatgc tgttcgtatt cgcatggtta aagcagataa caagcgtgga
     5041 acgtctatca cagaggtaca aatctttgcg aaacaagttg cggcagccaa acaaggacaa
     5101 ataagaatcc aagttgacgg caaagactta gcaaacttca accctgattt gacagactac
     5161 taccttgagt ctgtagatgg aaaagttccg gcagtcacag caagtgttag caacaatggt
     5221 ctcgctaccg tcgttccaag cgttcgtgaa ggtgagccag ttcgtgtcat cgcgaaagct
     5281 gaaaatggcg acatcttagg agaataccgt ctgcacttca ctaaggataa gaacttactt
     5341 tctcataaac cagttgctgc ggttaaacaa gctcgcttgc tacaagtagg tcaagcactt
     5401 gaattgccga ctaaggttcc agtttacttc acaggtaaag acggctacga aacaaaagac
     5461 ctgacagttg aatgggaaga agttccagca gaaaatctga caaaagcagg tcaatttacc
     5521 gttcgaggcc atgtccttgg tagtgatctt gttgctgagg tcactgtacg agtgacagac
     5581 aaactaggcg aagctctttc agataaccct aactatgatg aaaacagtaa ccaggccttt
     5641 gcttcagcaa ccaatgatat tgacaaaaac tctcatgacc gcgttgacta tctcaatgac
     5701 ggagatcatt cagaaaatcg tcgttggaca aactggtctc caacaccatc ttctaatcca
     5761 gaagtatcag cgggtgtgat cttccgtgaa aatggtaaga ttgtagaacg gactgttgcc
     5821 caagccaaac ttcgtttctt tgcagatagt ggtacggatg caccaactaa actcgtttta
     5881 gaacgctatg tcggtccaga gtttgaagtg ccaacctact attcaaacta ccaagcctac
     5941 gacgcagacc atccattcaa caatccagaa aattgggaag ctgtgcctta tcgtgcggat
     6001 aaagacatcg cagctggtga tgaaatcaac gtaacattta aagctgtcaa agccaaagct
     6061 atgagatggc gtatggagcg taaagctgac aagagcggtg ttgcgatgat tgagatgacc
     6121 ttccttgcac cgagtgaatt gcctcaagaa agtacgcaat cgaagattct tgtagatgga
     6181 aaagaacttg ctgatttcgc tgaaaatcgt caagactatc aaattaccta taaaggtcaa
     6241 cggccaaaag tctcagttga agaaaacaat caagtagctt caactgtggt agatagtgga
     6301 gaagatagcc ttccagtact tgttcgcctc gtttcagaaa gtggaaaaca agtcaaggaa
     6361 taccgtatcc agttgactaa ggaaaaacca gtttctgctg tacaagaaga tcttccaaaa
     6421 ctcgaatttg ttgaaaaaga tttggcctac aagacagttg agaaaaaaga ttcaacactg
     6481 tatctaggtg aaactcgtgt agaacaagaa ggaaaaactg gtaaagaacg tatctttaca
     6541 gcgattaatc ctgatggaag taaggaagaa aaactccgtg aagtggtaga agctccgaca
     6601 gaccgcatag tcttggttgg aaccaaacca gtagctcaag aagctaaaaa accacaagtg
     6661 tcagaaaaag cagatacaaa accaattgat tcaagtgaag ctagtcaaac taataaagcc
     6721 cagttaccaa atacaggtag tgcggcaagc caagcagcag tagcagcagg tttagctctt
     6781 ctaggtttga gtgcaggatt agtagttact aaaggtaaaa aagaagacta gaaattttga
     6841 taccttcttt atcattaaag accaaagaaa aactcttgtc ctaaatagaa gataaagagt
     6901 ctggaacaaa atagttctca accacaaaaa agctagatat ttccaattgc ggaactctag
     6961 ctttttaatt ttgagtcgtt ctcttattgt atagtagatt gaaactagaa tagtacactt
     7021 ctacttctaa aacattgtta gaaatcaatt tgactgtcct tatcgatttg tcctgttctt
     7081 atttcatttc actat
//
LOCUS       DAVBCN010000084         7061 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVBCN010000084 DAVBCN010000000
VERSION     DAVBCN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.084"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(52..1113)
                     /locus_tag="VQ497_001788"
     CDS             complement(52..1113)
                     /locus_tag="VQ497_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET2518068.1"
                     /translation="MNKRVQAFLAKMQEKELDGVIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            complement(1106..3937)
                     /gene="uvrA"
                     /locus_tag="VQ497_001789"
     CDS             complement(1106..3937)
                     /gene="uvrA"
                     /locus_tag="VQ497_001789"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET2518069.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAIKASSVEQIVDKVLELPERQRLQILSPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDNSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPNASKTLHEGALAPWNPISSNYYPNMLEQAMTAFGVAMDKP
                     FEDLSEEDKNLILYGSDGKKFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCHGYRLNDQALSVRVGGEQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            4069..5013
                     /locus_tag="VQ497_001790"
     CDS             4069..5013
                     /locus_tag="VQ497_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET2518070.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            5025..5708
                     /locus_tag="VQ497_001791"
     CDS             5025..5708
                     /locus_tag="VQ497_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HET2518071.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASVFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            5963..6994
                     /locus_tag="VQ497_001792"
     CDS             5963..6994
                     /locus_tag="VQ497_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HET2518072.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGRALFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNAGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
BASE COUNT         1990 a         1666 c         1437 g         1968 t
ORIGIN      
        1 aatttttttt gacttttttc gtcattttcg tacttttttc ttgacaaaca actaaatgac
       61 tatcaactct tttggagcta gggtcaataa ttcacaacct gtctctgtaa tcaggatatc
      121 atcctcgata cgaacgccat atttgccttc gatatagata cctggttcat cggtcaaggc
      181 catacctgtc ttaatagttt ctgtagaagt ctgactaaag tagggttcct catggatatc
      241 cagaccaata ccgtggccaa tgccgtgagt aaagtagtca ccataacctg cctcaatgat
      301 aatatcacga gggattttgt caaagtcacg gaaacctaag cctgccttag cttggtcaat
      361 caaggcttgg ttagctttta gaaccgtatt gtaaatctct gcctgctcat cgctaacatg
      421 ccctagatag atagtccggg tcatatcact gacatagtgg tcatagagac agccgaagtc
      481 catggtaatg gcttctccca actccactgg tttgtgcatt ggatgggcat ggggtttaga
      541 agaattgata ccgctagcta ggatcgtatc aaaagataag ccagatgctc ccaactcacg
      601 catgcggaaa tcaaggaagt tggcaatctc aatttcagtt tttcctggtt tgataaagtc
      661 aagcgcatcg cggaaagctt ggtctgagat agaacaagcc ttgcgaatcg ctgcaatctc
      721 tgcctcatcc ttaatcatac gaagaccttc cacaaactga gtttgtggaa gcaagttcaa
      781 acctgcaaaa gctgcctgca tacggtggta ataagacact gaaatctcat cttcaaaacc
      841 gatacgagtc aagcccatgt ccttaacaat tcctgcaatg acagccaatt catcacgatc
      901 agccacaatc tcaaaaccac tggtttcttg cttagctgcg atgatatagc gagagtctgt
      961 cactaatacc tgacggtcac ggctgataaa gactgttccg tttgagcccc aaaaaccagt
     1021 caaataatag acgtttttaa gattgttgat gatgacacca tctagttctt tttcttgcat
     1081 tttagctaga aatgcttgta cgcgtttatt catgatgtaa ctttcctttc aaatagtgtc
     1141 ctgtatagct ggcttcgttg gcagctactt cttctggagt tcctgttacg atgatggttc
     1201 caccaccgac accgccctca ggtcccaagt caatgatatg gtctgccgtc ttgataacat
     1261 ccagattgtg ctcgatgacg aggactgtat tgccatcgtc tacaaagcga gctaaaacct
     1321 tgagcaggcg agcaatgtcc tctgtatgaa gccctgtcgt cggttcatcc agaatgtaga
     1381 aagattttcc tgtcgagcgt ttgtggagtt cgctagctaa cttcatacgt tgggcttctc
     1441 ccccagaaag ggtggtagct ggctgtccca aggtcacata gcctagccct acatccttga
     1501 tggtctggag tttgcgttga attttcggaa tgtgttggaa aaattctacc gcatcgttga
     1561 ccgtcatatc caagacctgc gaaatattct tttccttgta gtgaacttct agggtttcac
     1621 tgttatagcg ggttccgtgg caaacttcac aagccacata aacatctggc aagaagtgca
     1681 tctcaatctt gataatcccg tcacctgagc aagcttcaca gcgacctccc ttgacgttga
     1741 aactgaagcg tcccttcttg tagcctcgaa tcttggcttc atttgtctga gcaaaaaggt
     1801 cacgtatatc gtcaaaaact cctgtatagg tagctgggtt agacctcggc gtccgtccga
     1861 tagggctctg gtcaatatca atcaaacggt cgacatgctc aatccctgta atagtcttaa
     1921 acttaccagg tttgtctgaa ttacggttga gcttctgggc aatggctttt ttgagaatgc
     1981 tgttgattag agtcgatttc cctgaacccg acacacctgt cactgcgata aattttccta
     2041 gtggaaagcg agccgtgaca ttttgcaagt tgttctcacg cgctcctatc acttcaataa
     2101 aacgaccatt tccgacacgg cgctcttctg gtactgggat gacacgtttg cctgacaagt
     2161 actgacctgt gatagacttg ctgttgcgag ccacttgctt aggtgtacct gctgcaacaa
     2221 tctcaccacc aaaaacaccg gcaccaggac caacgtcaat cagataatca gcttcacgca
     2281 tggtatcttc gtcgtgttcc accacgataa gagtattgcc caagtcacgc atctttttca
     2341 gactggcaat caggcggtca ttgtctctct ggtgaagacc gattgacggc tcgtctagga
     2401 tatagaggac gcctgatagg ttggaaccaa tctgggttgc caaacgaatg cgctgacttt
     2461 ccccacctga aagggttcct gctgaacgtg acagggttag atagttaaga cccacattat
     2521 taaggaaggt caaacgatcc ttgatttcct tgagaatggg acgagcaatg atggcttcat
     2581 tttcagacaa agttaactgg ctcaccaagt ccaagtgatc tgcgatagac aggtctgaaa
     2641 tttctccgat atgtggccct tgctcaccac ccacacggac agacaaggcc tggtcattga
     2701 gacgataacc gtgacaggtt ccgcaagtca gctcattcat gtagaggcgc atctgagtgc
     2761 gagtgtaatc gctattggtt tcatggtaac gacgtttgat attattgata actccctcaa
     2821 acggaatgtc gatatcgcgc acgccaccaa attcattctc atagtggaaa tggaatttct
     2881 taccatctga gccgtagaga atcaagttct tgtcttcttc tgacaagtcc tcaaaaggct
     2941 tatccatagc cactccaaaa gccgtcatgg cctgctctag catgtttgga tagtagttgg
     3001 atgagatagg attccaaggt gccagcgctc cctcatgtaa ggttttgctg gcatttggca
     3061 ctaccaaatc agtatccacc tccagcttga tgcccaagcc gtcacactca ctacaagagc
     3121 caaaaggagc attgaaagaa aagagacgag gctctaactc tgggacagta aaaccacaaa
     3181 ctggacaggc ataatgctca gagaacaaca actcagagtt gtccatggtg tcgataatga
     3241 cataaccttc tgcaatacga agggcagcct caatggaatc aaagagacga ctacgaatgc
     3301 cctccttgat aacaatacgg tcaaccacga catcgatatt gtgttgcttg ctcttagaca
     3361 actctggcac ttcggtcaca tcatagactt ccccatccac acggacacga acatacccgt
     3421 ctttctgaac cttctcgata acactcttat gttggccttt tttcttgcgg atgacaggag
     3481 acaagatctg caagcgctgg cgttcaggta actccaaaac cttatcaacg atttgctcca
     3541 cagaagaagc cttgatagcc ccatgcccgt tgatacagta aggcgtcccc acacgcgcgt
     3601 agaggagacg cagatagtca ttgatttcag tcgttgttcc caccgtcgag cgagggtttt
     3661 tgctagtcgt tttctggtcg atagaaatag ctgggctgag accatcaatg gcatctacat
     3721 ctggtttttc catatttccc aagaactgac gagcgtaggc ggacaaactc tctacatagc
     3781 gacgttgtcc ctccgcatag agggtatcaa aagccaaact ggacttccct gaacctgaca
     3841 agccagtcac gacaaccaac ttgtctcgcg gaatctccac atcaatattt tttaaattat
     3901 gggcacgcgc cccatgaatg acaattttat cttgcatctt tgttctttct agtccattat
     3961 tgcttaccat tataccaaaa aaagtgagat tctattaccc aaaaggccga ttttgtagta
     4021 taatagtaca gtgtgaaaaa atctgaaaaa tgagaaagga taagggatat gaaacaagtt
     4081 tttctctcta caacaactga atttaaagag atcgatacgc ttgaaccggg tacttggatc
     4141 aatctcgtca atccgactca aaatgaatca ctcgaaatcg ccaacacctt cgatattgat
     4201 attgccgacc ttcgagcacc gctcgatgcg gaagaaatgt ctcgtattac cattgaagac
     4261 gagtataccc tgattatcgt agacgtgccg gtcacggagg aaagaaataa ccgcacctac
     4321 tacgtaacca tcccgcttgg tattatcatc actgaggaaa ccattatcac tacgtgtttg
     4381 gaaccactac ctgtccttga tgtctttatc aaccgtcgat tgcgtaattt ctataccttc
     4441 atgcgttcac gttttatctt tcaaattctt tatcgcaatg cagagcttta cctaacagcc
     4501 cttcgttcaa tcgaccgcaa gagtgaacaa atcgaaagtc aactgcatca atcaactcgt
     4561 aatgaagaat tgattgagct catggaattg gaaaaaacta tcgtctattt caaggcctcc
     4621 ctcaaaacaa atgagcgcgt gattaagaaa ttgaccagtt caaccagcaa tatcaagaaa
     4681 taccttgagg acgaagacct gcttgaagac accctgattg aaacccaaca ggccatcgag
     4741 atggcagata tttatggaaa cgtcttgcat tctatgacag agacctttgc ctctatcatt
     4801 tctaacaacc agaacaacat catgaaaacc ttggcccttg tgaccatcgt catgtccatc
     4861 ccaaccatgg tcttttctgc ctacgggatg aactttaagg ataatgaaat tcccctaaac
     4921 ggagagccaa atgccttctg gttaatcgtc tttatcgcct ttgctatgag tgtctcgctc
     4981 actctctatc tcatccataa aaaatggttc taaaaggagt tcctatgtct caaattgatc
     5041 tacaaaaatt aactaagaaa aaccaagagt ttgtccacat tgctacccaa caattcatca
     5101 aagatgggaa aacagacgct gaaatccaga ctatttttga ggaagtcatt ccccaaatcc
     5161 ttgaggagca atctaaaggt acaactgccc gttccctata cggcgcacca actcattggg
     5221 ctcatagctt cactgtcaaa gagcagtacg aaaaagagca tccaaaagaa aatgatgacc
     5281 caaaactgat gattatggac tcagctcttt tcatcactag cctctttgcc cttgtcagcg
     5341 ccctcacaac cttctttgcg gcagatcaag ctttcggcta tggattgatt actcttctat
     5401 tagttggact ggttggtgga tttgccttct acttgatgta ctactttgtt taccaatact
     5461 atggaccaga tatggatcgc agtcaacgtc cacctttctg gaaatctgta ctagttatcc
     5521 tagcttctgt gttcctttgg ttgcttgtct tctttgcaac aagcttccta ccagctagcc
     5581 ttaacccagt actggatcca ttgccactag ctattattgg agcagccctc ctagcccttc
     5641 gcttctatct caagaaacgc ttgaatatcc gtagtgcaag tgcaggacca acacgctatc
     5701 aagaataaga aaacgataaa agcaactgca ggtgcggttg ctttttcact tacttttttg
     5761 agttatactc aatgaaaatc aaagagcaaa ctaggaagct agctgcaggt tgctcaaagc
     5821 acagctttga ggttgcagat aaaactgacg tggtttgaag agattttcga agagtattaa
     5881 aagtattctt ctgaaatccc acatagcttt ctcttatatt ttgtgataaa ataggctcaa
     5941 tctatttcta ggaggatgag atatggtttc tactattggt attgttagtt tatctagtgg
     6001 cattatcgga gaggattttg tcaaacacga agtggacttg ggtatccaac gtctcaagga
     6061 tctgggactc aatcccatct ttttgcccca ttcgttaaaa ggattagact ttatcaagga
     6121 ccatcctgaa gctcgtgcag aggatttgat tcatgccttt tctgatgata gcatcgacat
     6181 gatcctatgt gccatcggtg gagacgatac ctatcgcttg ctaccttatc tttttgaaaa
     6241 tgaccaactc caaaaggtta tcaagcaaaa aatttttctt ggcttctcgg atacaaccat
     6301 gaaccacctc atgttgcata aattaggaat caagactttt tatggtcaat cctttttagc
     6361 agacatttgt gaattagaca aagaaatgct agcctatagc cttcactact ttaaagaatt
     6421 gattgagacg ggaagaattt cagaaatccg ccctagtgac gtttggtatg aggaacgaac
     6481 tgattttagt cccacggccc tgggaacacc tcgtgtcagt catacaaata caggttttga
     6541 cttgttgcaa ggaagggcac tattcgaggg gaaaatcctc ggtggttgcc tcgaatccct
     6601 ctacgatatc tttgacaact ctcgatacgc agatagcacg gagctctgcc aaaaatacaa
     6661 acttttccct gacttgtcag actgggaagg gaaaatcctc ttgctcgaaa caagcgaaga
     6721 aaagcctaag ccagaagact tcaaaaagat gttgctaact ttaaaggaca ctggcatatt
     6781 cgcggtcatc aatggactct tggtcggaaa acctatggat gaaactttcc atgacgacta
     6841 taaagaggca ctattggata tcattgacag caatatcccg attgtctata atctgaatgc
     6901 cggccacgca actccaagag caattgtacc ctttggggtc catgctcatg tagatgcaca
     6961 ggaacaagtc attcgctttg actataacaa ataaagctgg gaaaagtttc tcagcttttt
     7021 tctcggtatc attactggca ttttcatcac atattacagc a
//
LOCUS       DAVBCN010000085         6989 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVBCN010000085 DAVBCN010000000
VERSION     DAVBCN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6989
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.085"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            226..6657
                     /locus_tag="VQ497_001793"
     CDS             226..6657
                     /locus_tag="VQ497_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET2518073.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     VLDKETENKEKHEDIHNAIETSQDFKEKKTAVIEGKEVVSKNPVTDNNTSNEEARIKE
                     DSNQSQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKQLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPYGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANHADEKG
                     MISYYLVDPDKDASKYEKLGEISEDKLKNAKSPEENTNNNQAKDEDSKPDEKSSVEGE
                     ASLEINKTISTIREFENKDLKKLIKKKFREVDDFTSETGKRIEEYDYKYDDKGNIIAY
                     DDGSALQYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIEI
                     KATKYDSHSKTMIFDLYANINDIVDGLAFAGDMRLFVKDDNQIKAETKIRMPEKNKET
                     KAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGY
                     ALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQ
                     AVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKS
                     NVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWE
                     LRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDG
                     NVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRN
                     NGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTG
                     EVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKD
                     AGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQ
                     REDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVG
                     IFLGLKKKNQD"
BASE COUNT         2759 a          943 c         1347 g         1940 t
ORIGIN      
        1 taatcttatt ttttaacgtg tgctaaaaaa taaattaatg catgttaatt tttcttgaat
       61 taaatttttt taaatgatat actgttttta tggagagata acaattatat ataaatgtac
      121 tactctattt cctagataat acttagtaaa actttttata acaaaggcta gcaaagttaa
      181 agtttattat ctattggaag ttaaataaat atgtaaggaa ttaaaatgaa aaaaagtaca
      241 gtattgtcat taaccacagc tgcagttatt ttagcagcct atgcccctaa tgaggtagtc
      301 ttagcagaca catctagctc tgaagatgct ttaaacatct ctgataaaga aaaagtagta
      361 ttagataagg aaacagaaaa taaagagaaa catgaagata ttcataatgc tatagaaact
      421 tcacaggatt ttaaagagaa gaaaacagca gtcattgagg gaaaagaagt tgttagtaaa
      481 aatcctgtga cagacaataa cactagcaat gaagaagcaa gaatcaaaga agattccaat
      541 caatcccaag gagatcatac ggactcattt gtgaataaaa acacagaaaa tcccaaaaaa
      601 gaagataaag ttgtctatat tgctgaattt aaagataaag aatctggaga aaaagcaatc
      661 aagcaactat ccaatcttaa gaatacaaaa gttttatata cttatgatag aatttttaac
      721 ggtagtgcca tagaaacaac tccagataac ttggacaaaa ttaaacaaat agaaggtatt
      781 tcatcggttg aaagggcaca aaaagtccaa cccatgatga atcatgccag aaaggaaatt
      841 ggagttgagg aagctattga ttacctaaag tctatcaatg ctccgtttgg gaaaaatttt
      901 gatggtagag gtatggtcat ttcaaatatc gatactggaa cagattatag acataaggct
      961 atgagaatcg atgatgatgc caaagcctca atgagattta aaaaagaaga cttaaaagga
     1021 actgataaaa attattggtt gagtgataaa atccctcatg cgttcaatta ttataatggt
     1081 ggcaaaatca ctgtagaaaa atatgatgat ggaagggatt attttgaccc acatgggatg
     1141 catattgcag ggattcttgc tggaaatgat actgaacaag atatcaaaaa ctttaacggc
     1201 atagatggaa ttgcacctaa tgcacaaatt ttctcttaca aaatgtattc tgacgcagga
     1261 tctgggtttg cgggtgatga aacaatgttt catgctattg aagattctat caaacacaac
     1321 gttgatgttg tttcggtatc atctggtttt acaggaacag gtcttgtagg tgagaaatat
     1381 tggcaagcta ttcgggcatt aagaaaagca ggcattccaa tggttgtcgc tacgggtaac
     1441 tatgcgactt ctgcttcaag ttcttcatgg gatttagtag caaataatca tctgaaaatg
     1501 accgacactg gaaatgtaac acgaactgca gcacatgaag atgcgatagc ggtcgcttct
     1561 gctaaaaatc aaacagttga gtttgataaa gttaacatag gtggagaaag ttttaaatac
     1621 agaaatatag gggccttttt cgataagaat aaaatcacaa caaatgaaga tggaacaaaa
     1681 gctcctagta aattaaaatt tgtatatata ggcaaggggc aagaccaaga tttgataggt
     1741 ttggatctta ggggcaaaat tgcagtaatg gatagaattt atacaaagga tttaaaaaat
     1801 gcttttaaaa aagctatgga taagggtgca cgcgccatta tggttgtaaa tactgtaaat
     1861 tactacaata gagataattg gacagagctt ccagctatgg gatatgaagc ggatgaaggt
     1921 actaaaagtc aagtgttttc aatttcagga gatgatggtg taaagctatg gaacatgatt
     1981 aatcctaata aaaaaactga agtcaaaaga aataataaag aagattttaa agataaattg
     2041 gagcaatact atccaattga tatggaaagt tttaattcca acaaaccgaa tgtaggtgac
     2101 gaaaaagaga ttgactttaa gtttgcacct gacacagaca aagaactcta taaagaagat
     2161 atcatcgttc cagcaggatc tacatcttgg gggccaagaa tagatttact tttaaaaccc
     2221 gatgtttcag cacctggtaa aaatattaaa tccacgctta atgttattaa tggcaaatca
     2281 acttatggct atatgtcagg aactagtatg gcgactccaa tcgtggcagc ttctactgtt
     2341 ttgattagac cgaaattaaa ggaaatgctt gaaagacctg tattgaaaaa tcttaaggga
     2401 gatgacaaaa tagatcttac aagtcttaca aaaattgccc tacaaaatac tgcgcgacct
     2461 atgatggatg caacttcttg gaaagaaaaa agtcaatact ttgcatcacc tagacaacag
     2521 ggagcaggcc taattaatgt ggccaatgct ttgagaaatg aagttgtagc aactttcaaa
     2581 aacacggatt ctaaaggttt ggtaaactca tatggttcca tttctcttaa agaaataaaa
     2641 ggtgataaaa aatactttac aatcaagctt cacaatacat caaacagacc tttgactttt
     2701 aaagtttcag catcagcgat aactacagat tctctaactg acagattaaa acttgatgaa
     2761 acatataaag atgaaaaatc tccatatggt aagcaaattg ttccagaaat tcacccagaa
     2821 aaagtcaaag gagcaaatat cacatttgag catgatactt tcactatagg cgcaaattct
     2881 agctttgatt tgaatgcggt tataaatgtt ggagaggcca aaaacaaaaa taaatttgta
     2941 gaatcattta ttcattttga gtcagtggaa gaaatggaag ctctaaactc caacgggaag
     3001 aaaataaact tccaaccttc tttgtcgatg cctctaatgg gatttgctgg gaattggaac
     3061 cacgaaccaa tccttgataa atgggcttgg gaagaagggt caagatcaaa aacactggga
     3121 ggttatgatg atgatggtaa accgaaaatt ccaggaacct taaataaggg aattggtgga
     3181 gaacatggta tagataaatt taatccagca ggagttatac aaaatagaaa agataaaaat
     3241 acaacatccc tggatcaaaa tccagaatta tttgctttca ataaccaagg gatcaacgct
     3301 ccatcatcaa gtggttctaa gattgctaac atttatcctt tagattcaaa tggaaatcct
     3361 caagatgctc aacttgaaag aggattaaca ccttctccac ttgtattaag aagtgcagaa
     3421 gaaggattga tttcaatagt aaatacaaat aaagagggag aaaatcaaag agacttaaaa
     3481 gtcatttcga gagaacactt tattagagga attttaaatt ctaaaagcaa tgatgcaaag
     3541 ggaatcaaat catctaaact aaaagtttgg ggtgacttga agtgggatgg actcatctat
     3601 aatcctagag gtagagaaga aaatgcacca gaaagtaagg ataatcaaga tcctgctact
     3661 aagataagag gtcaatttga accgattgcg gaaggtcaat atttctataa atttaaatat
     3721 agattaacta aagattaccc atggcaggtt tcctatattc ctgtaaaaat tgataacacc
     3781 gcccctaaga ttgtttcggt tgatttttca aatcctgaaa aaattaagtt gattacaaag
     3841 gatacttatc ataaggtaaa agatcagtat aagaatgaaa cgctatttgc gagagatcaa
     3901 aaagaacatc ctgaaaaatt tgacgagatt gcgaacgaag tttggtatgc tggcgccgct
     3961 cttgttaatg aagatggaga ggttgaaaaa aatcttgaag taacttacgc aggtgagggt
     4021 caaggaagaa atagaaaact tgataaagac ggaaatacca tttatgaaat taaaggtgcg
     4081 ggagatttaa ggggaaaaat cattgaagtc attgcattag atggttctag caatttcaca
     4141 aagattcata gaattaaatt tgctaatcat gctgatgaaa aggggatgat ttcttattac
     4201 ctagtagatc ctgacaaaga tgcttctaaa tatgaaaaac ttggtgaaat ttctgaagat
     4261 aaactcaaaa atgcaaagag cccagaggaa aataccaata ataatcaagc taaggatgaa
     4321 gattcaaaac cagatgaaaa aagttcagtt gagggagaag ctagccttga aataaataaa
     4381 actatttcaa caattagaga gtttgaaaat aaagacctaa agaaactcat taaaaagaaa
     4441 tttagagaag ttgatgattt tacaagtgaa actggtaaga gaatagagga atacgattat
     4501 aaatacgatg ataagggaaa tattattgct tatgacgatg gtagtgcctt acaatatgaa
     4561 actgaaaaac ttgacgaaat aaaatcaaaa atttatggtg ttctaagccc atctaaagat
     4621 ggacactttg aaattcttgg aaagataagc aatgtttcta aaaatgccaa ggtatattat
     4681 ggaaatagct ataaatcgat agaaatcaaa gcgactaagt atgattcaca ttcaaaaacg
     4741 atgatatttg atttatacgc taatattaat gatattgtag atggattagc ttttgctgga
     4801 gatatgagat tatttgttaa agatgacaat caaataaaag ctgaaactaa gattagaatg
     4861 cctgaaaaaa ataaggaaac taaagcagaa tatccctatg tatcaagtta tgggaatgta
     4921 atcgaattag gagaaggaga tctttcaaag aacaaaccag acaatttaac taaaatggaa
     4981 tctggtaaaa tctattctga ttcagaaaaa caacaatatc tgttaaagga taacatcatt
     5041 ctaagaaaag gctatgcact aaaagtgact acctataatc ctggaaaaac ggatatgtta
     5101 gaaggaaatg gagtctatag caaggaagat atagcaaaaa tacaaaaggc caatcctaat
     5161 ctaagagtcc tttcagaaac aacaatttat gctgatagta gaaatgttga agatggaaga
     5221 agtacccaag ctgtattaat gtcggctttg gacggcttta acattataag gtatcaagtg
     5281 tttacattta aaatgaacga taaaggggaa gctatcgata aagacggaaa tcttgtgaca
     5341 gattcttcta aacttgtatt atttggtaag gatgataaag aatacactgg agaggataag
     5401 tccaatgtag aagctataaa agaagatggc tccatgttat ttattgatac caaaccagta
     5461 aacctttcaa tggataagaa ctactttaat ccatctaaat ctaataaaat ttatgtacga
     5521 aatccagaat tttatttaag aggtaagatt tctgataagg gtggttttaa ctgggaattg
     5581 agagttaatg aatcggttgt agataattat ttaatctacg gagatttaca cattgataac
     5641 actagagatt ttaatattaa gctgaatgtt aaagacggtg acatcatgga ctggggaatg
     5701 aaagactata aagcaaacgg atttccagat aaggtaacag atatggatgg aaatgtttat
     5761 cttcaaactg gctatagcga tttgaatgct aaagcagttg gagtccacta tcagttttta
     5821 tatgataatg ttaaacccga agtaaacatt gatcctaagg gaaatactag tatcgaatat
     5881 gctgatggaa aatctgtagt ctttaacatc aatgataaaa gaaataatgg attcgatggt
     5941 gagattcaag aacaacatat ttatgtaaat ggaaaagaat atacatcatt tgatgatatt
     6001 aaacaaataa cagacaagac actaaacatt aagattgttg taaaagattt tgcaagaaat
     6061 acaaccgtaa aagaattcat tttaaataaa gatacgggag aggtaagtga attaaaacct
     6121 catagggtaa ctgtgaccat tcaaaatgga aaagaaatga gttcaacgat agtgtcggaa
     6181 gaagatttta ttttacctgt ttataagggt gaattagaaa aaggatacca atttgatggt
     6241 tgggaaattt ctggtttcga aggtaaaaaa gacgctggct atgttattaa tctatcaaaa
     6301 gataccttta taaaacctgt attcaagaaa atagaggaga aaaaggagga agaaaataaa
     6361 cctacttttg atgtatcgaa aaagaaagat aacccacaag taaaccatag tcaattaaat
     6421 gaaagtcaca gaaaagagga tttacaaaga gaagatcatt cacaaaaatc tgattcaact
     6481 aaggatgtta cagctacagt tcttgataaa aacaatatca gtagtaaatc aactactaac
     6541 aatcctaata agttgccaaa aactggaaca gcaagcggag cccagacact attagctgcc
     6601 ggaataatgt ttatagtagg aatttttctt ggattgaaga aaaaaaatca agattaagac
     6661 aaaagctata gaaaaaaatg gtttatgtac tgagattaga tagtgaggtg atgacatagt
     6721 tttgtgaaaa cagccattta taactcaatt atttagttta cttcactatt gatactattt
     6781 ggagttatta atggacttag tttagataac taatgaattg attgaaaggg ttagtattga
     6841 caatattggt catattgatt agaaaataga gtctatcaaa atttaaaggc taatagaggt
     6901 gatgagacaa tttcggctct ttgtcaactg tagtgggttg aagtcagcta agctcgagaa
     6961 aggacaaatt ttgtcctttc ttttttgat
//
LOCUS       DAVBCN010000086         6795 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVBCN010000086 DAVBCN010000000
VERSION     DAVBCN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.086"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(<1..207)
                     /locus_tag="VQ497_001794"
     CDS             complement(<1..207)
                     /locus_tag="VQ497_001794"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518074.1"
                     /translation="MENMTNPLLLVQIRVHGYLLDFASSEGQRQKHYRLKNLPQTVEL
                     TVDDVEEDVDLTLPENRSYQEADFF"
     gene            complement(275..916)
                     /locus_tag="VQ497_001795"
     CDS             complement(275..916)
                     /locus_tag="VQ497_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2518075.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(918..2948)
                     /locus_tag="VQ497_001796"
     CDS             complement(918..2948)
                     /locus_tag="VQ497_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2518076.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEVLSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLL
                     SLAIFLLTFMSLPLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQFICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSTYYQRLFTDYL
                     QNFSSESVEVTSQKYYLPQVSLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(3217..4116)
                     /locus_tag="VQ497_001797"
     CDS             complement(3217..4116)
                     /locus_tag="VQ497_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2518077.1"
                     /translation="MLIGQKIKEIRIEKGVSRPYFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPPAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMSQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(4188..4748)
                     /gene="rnmV"
                     /locus_tag="VQ497_001798"
     CDS             complement(4188..4748)
                     /gene="rnmV"
                     /locus_tag="VQ497_001798"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HET2518078.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINAQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVRHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(4748..5521)
                     /locus_tag="VQ497_001799"
     CDS             complement(4748..5521)
                     /locus_tag="VQ497_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET2518079.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     RALELVDEYDQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKILVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(5826..6719)
                     /locus_tag="VQ497_001800"
     CDS             complement(5826..6719)
                     /locus_tag="VQ497_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HET2518080.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLENTPGGDKGGNTGSSDANANAGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIVMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
BASE COUNT         1908 a         1619 c         1173 g         2095 t
ORIGIN      
        1 aaaaaaatca gcttcttgat aacttcgatt ttcgggtagg gtcaaatcca catcctcctc
       61 cacatcatcc actgtcagtt caaccgtttg aggtaaattt ttcaaacgat aatgcttttg
      121 cctttgccct tcactactag caaaatcaag caaatagcca tgcactcgga tttgaaccaa
      181 aaggagagga ttggtcatat tttccatgat gaggatatct tataaaccac ctctggcact
      241 aggataatca gataccctgc gaatacggat tttttcacac atgagcaagt ttcctcatat
      301 caatgatttc atcagcctta ttccagacta ggggattatg ggtcgcaatg atgataattc
      361 gattttcatc tttcaaatcc accaagagat tcataacctc ctctgaattt tcaggatcaa
      421 gagctgctgt tggttcatct gccaaaatca agggtggatt tttcaaaata gttttcgcaa
      481 gggcaactcg ttgggcctct cccccagata aagtataaat tttttgttct aaatccaaat
      541 accctagatt aactttttct aaagccccca cttgcctttc caaacgttct acttttgaga
      601 ttttctgacc aacaaaaccc aaatccaaat tttctttgat tgattggttt tctaagaggc
      661 caaaattttg aaagagatag cccatctgat ctcgaaaata ctcacgagtg ggaatggttt
      721 ttaaatcttt cccctgatag agaacccttc ctccatctat cttttctagc tttcccaaga
      781 tattcagcag cgtcgtcttt ccgcttccac tctttccgat taaggcataa accttgccct
      841 tctccagctt gagattcaaa ccagagaaaa tcgcgcggtc attaaatttc ttttccaatc
      901 cttgaatatc aatcatctta ttttcctttc aatactgcca tgctaccagc ttcttctttt
      961 ttgtcctgcc ttactaaaat caagagggcg ttaagcgtaa acaaagctac aactagagcg
     1021 ctaatcaaac catctcttgt caaaatacta gataggacta ggccaagcaa gagaactcct
     1081 ccttgcgcca gtaaatactt gccatgaagc tcatagattg tcataccagc aagacgttta
     1141 atcataagct cccgtctgaa ctgctcaaaa tagagaagat tcatggaatc aaataacaag
     1201 atagccgtag acagggtcaa aatcgtccca ataaggagag agtaaaactc tacccgtttg
     1261 tcatttagta ccttggcaaa aaatagctgg ctttttacca agtaagaaac cttgtgatac
     1321 agaccttgct cttttagagc tgtgatgctg tctccatatc gatctagttt caaggcacta
     1381 ttagccgtag ttccccacaa catccctgca acaggtcttg ttccagtcgc ttgcggcgtt
     1441 agaactacaa taatcggatc ttttaggtac tggcgtgttg tcttgtaccc atcattatag
     1501 aggaaacgtt cctgtcctgt ttctgtaaaa gctagactta cctgtgggag gtagtatttc
     1561 tgactcgtca cttctacact ttcagatgaa aagttttgca ggtaatctgt aaacaatctt
     1621 tggtagtagg tagactgctc tcgcaagctc tcaggcaaga ttagcccaaa ctctccctca
     1681 gacaagtttt gcatcttgtc cataaactct gaagaaacgg taatcttttc ttctatcaga
     1741 tagcgtggtg agacatagat aacattccct gacggtgtgt agtcactgag acgattgcca
     1801 tctaggtcca cttctgctcc gtctgagaaa ttgtcaacat tgctcataat ataaaaagag
     1861 tctgtattgg ctaaccgttc ttcagtaaat gtctgccact cacgattatc cttacgcgtt
     1921 ccttcttcat cggcaaatgc actagaccaa ccaaaggata gacggtaacg gtctgaggac
     1981 tggctccatt tattgctagc tctctccatt tcctgcattt cacggtagtg gggtaggaga
     2041 gctgtcgcac tcgatccgac caccaataca gctaagagtt gccccaccat catcaatgtc
     2101 atcatacgtt tgagagggag tttccctttc aatagatcca ccagactatt ttcctgtaaa
     2161 ccaagtagat agacgacact tagtaaggta gaaatccctg ccagggtcaa tccataaagt
     2221 agaagagcaa tgatgaccag ttgcaccgtt gccataaaca aggcaccttg ataccagaga
     2281 atccccaatc ccaaaagact ggataccagc actgagcaga taaactgtct cacatcttct
     2341 aacactggtc tgagagcaac tccaaacaag ctctcaccag ctattaagcg aatccctgcc
     2401 tgacgaaggg atttgatccg ataaatcagg ggcagactca taaaggtcag cagaaaaata
     2461 gctaaactca gtagcaccat aggggtggcc gccaatagta agactatcga aaatggatct
     2521 tctccattcg aaacaaagcc ttgatagcca agttctttca aggtggtctg taagctcact
     2581 ccatccaaac ttcctgacac aatcaagtaa gaccctaata aatcactcgt ttctgcactc
     2641 tccttggagg aaatggtcag accttctgga agctctccct caccataagt ggcataggta
     2701 aagagtgttt ctccagcttc atttggctca acgattcgac gggcgatgag actattatgc
     2761 tctaccgcca agtgactcaa aacctctgtc acctcttcgc gactagctac tggttgattt
     2821 atcatcattc ctgtcacatc aacagaagga tagttaccaa ataaaatctc ttctcgatgg
     2881 tcaacggaag ttatcaccat ataaagcgat accaatacca ttgaaatcaa aataaataaa
     2941 cgtttcatca tttcctccaa aaagttactt ggtcacttag tgagaaggtg accacagtca
     3001 ctgcgatgtt aagaagctta ttaagttctt tctactagca acatcttcaa atacattata
     3061 tccgtttttt caagaactat caaggacatc aatgtcctta aaatctaact atttcatatt
     3121 atttttagct tatatctaaa aaaacagttc taaagacgaa ctttagaact gttttgatac
     3181 gaggacaagg acatttttgt cactgattca ctatgtttat aaatattttt tgacatcctc
     3241 ttgccattcc tctgtcaatt tctttactag aaatccatct ccaattagct tcgcaaatgt
     3301 tttagaagac tgataaaatg attcagctaa actaaaatct ctttcttctc ttaatgcttg
     3361 cttccacaaa aacataaata caagaggttt cttttgataa tcttgagttt tatccataat
     3421 ttcttgtaaa cacgatagat aatcattaaa acaatcagtt actcctacct tttcaagaca
     3481 acacattcct gaaataagag tatctctaat tataaagaag ttttctaatt tagttacctt
     3541 tttctgttcc aaaagaaaga gcattagctt ttcgatttga tttataaatt tggcaagatc
     3601 ttttctaatg agcatttgat aaaaaaacaa acgaatcaac aacatatcat ttgttgaata
     3661 aacttctttt tctatgatct gagacatatg ttcctcaagt attaactctg catattcagg
     3721 ccttctagtt ctagaagtat ccaaacacgc ctgcaatact tcacatgcta attgttcttc
     3781 tttaggaaga ttatcaaagt atgttttata aatctcttcc aaacacgctt ccttcttatc
     3841 gtactcctct tctttaccat agattggttc acgtaaaatc tggtatttca attctaaata
     3901 agcaggagga agagctgaaa aatccggcat aaggctataa actggaactc ctagccggcg
     3961 agcaatatag gctaacttgg gcaaactcgg ttgcgaagct ccactttcaa ttcgcgacag
     4021 ttgacgaact gtcagttctt gctcatctcc acaaaaatat ggacgactaa ctcctttttc
     4081 tatccgaatc tctttaattt tttgaccaat taacatcaga cgcctcctct tctacctgct
     4141 tcgaaaaata ctgtgaaact taaaaaattg taacggctac atctttccta ctcataagat
     4201 ttcatagctt cttccacttc tgccaaagta accccaaaca actctaggcg tttgaggagt
     4261 tgcttgccgt tagaatagcc gattcggaga gtctctccga gatattctct acgcttacgg
     4321 ctgtctgccc ctgctagaaa accaaggcga atcaagtcgc tacgactaat gtcaaactga
     4381 ctctcatgtt caaattgttc tgtcacctga gctagagccg ttttcaggtc ttcatagctg
     4441 gcatgctcaa ttcccagaga acgccccttg gtcttggact ttggaacagc ttcatctcgc
     4501 ttgagaaagg catgccgaac tgttggaatg gccgtcataa tcatgcgccg aatccgctcc
     4561 ccattaaaat ctgggtctgt aaagacaatg actccatgac gttggtgcag gcgctgaatc
     4621 cgctctatat cctgagcatt gatggcagaa cctcgagtct cataggtctc cacatcgaaa
     4681 taacgtttga gattgaccgt atcatcacgc ccttcaacca cgataacttg agaaattttc
     4741 tctttcatta cttgctgtcc aatccaaaaa tgcgttctgc atttgcagtc gttgctaccg
     4801 ccagctcttc tgtcgtcata ccacgcaagt cagcgataaa gtcgaccaca tagcgagtat
     4861 aggctgtttt attttcacga ccacgcttgg gtacaggtgc taagtaaggc gcatctgttt
     4921 ccaccaaaat cttgtccaaa ggtaactctt tagctgcttc ttggaggtca gttgccttct
     4981 taaaagtcac cactcctgag aaggaaatgg tcataccaag atccacaaac ttctctgccc
     5041 actcaagcgt ccctgaaaat gaatgcatga taccaccacg aggaccaacg ccctcactct
     5101 tgataatctc ataggtatct tccagcgcat cacgggtatg gacaacaaaa ggcaaatcca
     5161 agtccttaga tagctgaatc tgacggcgaa aaacctgctc ctgcacctct ttgggcgctg
     5221 tcatccaatg gtaatctaag ccaatttcac ctaaagccac aaccttggga tgttttaact
     5281 tatccaacaa gtaagcctca acttcctctg tataagtacc agcttctgta ggatgccaac
     5341 caatagtcgc atagagctga tcatactcat ctaccaactc caaggcacgc tcaatcgtcg
     5401 gtttatcaaa accaacaata ttcatctgtg tcacacccat ctcagcagcc aaggcaattt
     5461 cttctgcctc acgacctgca aattcttcta cattcaagtg tgtatgtgta tcaaaaatca
     5521 tctcttctaa cctcgttttc tatcttctat tataccaaaa aagaggaggg gcacctaatt
     5581 tttcggtttc ccctcctctc ttcaatagag agctattctg ctatcttttc tatccgatat
     5641 tgcccatctc ctattccaca gttagagaca gaagagattg gctacattga tagaatattc
     5701 ctattcagct aaagtatgtg attatcttgg gttctcactt ttctcaccca tcactaggat
     5761 tgatgacttc tcaaaatccc ccttataatc taactttaag tagagaatag aattttctag
     5821 ctgttctatt ttctacgtct agtctttctt cctagcaaag caaaaccact tgttgccacc
     5881 actagaccaa gactagcaag tgctgcactt gatgctgaac ctgtgttagg taattgtttg
     5941 ccagcaggtg actggtaggt aacatcttgc attgttcctg cagttgcttg cggtgcactt
     6001 gttgtcttag gagcagcatt aggagccact ggtctcttag gaactgtaac catgacaatg
     6061 gcttgcactc caatcgtttc tgcttccttc acatcttcca tagttttcgc attttcaatc
     6121 tctttgagag ctgcttgttt ttctgcttcc actcttgcta aaagttctgc tttttcttta
     6181 tcagaaagcg gtgcgccttt gatttcatcc tgcttgtcct tggctgcttt ttcaatggca
     6241 tttttagctg attctttttc agtagctaat tgcttagaag cttgtgattg agctgagttt
     6301 tgtgaacctg tgtgagctga tccacccgcc tggctaccac cgcctgcatt cgcattagca
     6361 tctgagcttc cagtattgcc tcccttatct ccacctggtg tattttctag cttatactct
     6421 ggtgtagctt cttgagttac agattcacct ttcgcagatt gttgaactgt atcttttcct
     6481 agaatcgtca ctactgaacc atctgggaat gtgattgttg ctgtaccatc tccagctaca
     6541 ttgattgtcg ctccgtctaa tgctgaacca tttgcttgta aaatagcaac cttaactttt
     6601 tcttcatctg ttaaatgagt tatatctttt acatctactc tttgagcagg tgctttaaca
     6661 gttgattttt cagcagtcgg tttagtgcta tcttgtgctt ttggatcttg tgctaccaat
     6721 tgatttcctg gaattgtaat agctgaacca tctttaaaga cgattgtcgc tgcgccatct
     6781 ccagctactt caact
//
LOCUS       DAVBCN010000087         6769 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVBCN010000087 DAVBCN010000000
VERSION     DAVBCN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.087"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(124..1266)
                     /locus_tag="VQ497_001801"
     CDS             complement(124..1266)
                     /locus_tag="VQ497_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET2518081.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETSSAVAEEAPQGTTPAEKQETQASPQAASAVEATTTS
                     SEAKEVASSNGATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            complement(1432..2052)
                     /locus_tag="VQ497_001802"
     CDS             complement(1432..2052)
                     /locus_tag="VQ497_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2518082.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQNLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNTFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            complement(2056..3336)
                     /locus_tag="VQ497_001803"
     CDS             complement(2056..3336)
                     /locus_tag="VQ497_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET2518083.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKLTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            complement(3397..4881)
                     /locus_tag="VQ497_001804"
     CDS             complement(3397..4881)
                     /locus_tag="VQ497_001804"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET2518084.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKGGVSKVQELQMTTQNGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTAELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVYESDS
                     YIEDPHTAVASAVYRKYQAVTGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            complement(4957..6234)
                     /locus_tag="VQ497_001805"
     CDS             complement(4957..6234)
                     /locus_tag="VQ497_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HET2518085.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            complement(6341..6424)
                     /locus_tag="VQ497_001806"
     tRNA            complement(6341..6424)
                     /locus_tag="VQ497_001806"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6388..6390),aa:Leu,seq:caa)
     gene            complement(6434..6505)
                     /locus_tag="VQ497_001807"
     tRNA            complement(6434..6505)
                     /locus_tag="VQ497_001807"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6471..6473),aa:Gln,seq:ttg)
     gene            complement(6519..6591)
                     /locus_tag="VQ497_001808"
     tRNA            complement(6519..6591)
                     /locus_tag="VQ497_001808"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6556..6558),aa:His,seq:gtg)
     gene            complement(6606..6676)
                     /locus_tag="VQ497_001809"
     tRNA            complement(6606..6676)
                     /locus_tag="VQ497_001809"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6642..6644),aa:Trp,seq:cca)
     gene            complement(6681..6761)
                     /locus_tag="VQ497_001810"
     tRNA            complement(6681..6761)
                     /locus_tag="VQ497_001810"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6725..6727),aa:Tyr,seq:gta)
BASE COUNT         2042 a         1596 c         1202 g         1929 t
ORIGIN      
        1 cagataaaac tgacgtggtt tgaagagatt ttcgaagagt atgacaaaga aaaaacgaac
       61 gagaatcgct tcccatcacg aaagctagat ctcattcgtc aaaatgatgt taccaagtcg
      121 ggtttatcca ttcattgaaa cgtgaacgtg atcatagtga ttttctgtca cactaccacg
      181 gtctggcatt gggttccaag tgttagctgg cccatatttg ctatcgaatg gagcatagaa
      241 acgttgtttc cagatgatgt aactaatgcc acggctggcc atgttttgaa tagcatattc
      301 cgcaatctta tcccctaatt ctgaacgttc tggtaccata aagtcgatag ccaaaccttt
      361 tccgtgatct ccactgtctc ctggacgata accgctaaag gatgtaatgc caaacaagtt
      421 agcaatttct tctttaaagg cagctgtttg tggttgaaga cctgcatttt cagattttgc
      481 tactgcaagt ccagcataat cgggcgcagc tggagcctcg taagttgttg aaattatttt
      541 cgtctcttct ggttgataag tagaaactgc tgctgtagct ccatttgatg atgctacttc
      601 ttttgcttct gaacttgttg tagttgcttc cactgctgat gcagcttgag ggcttgcttg
      661 tgtttcctgc ttctcagctg gagtcgttcc ctgaggagct tcttctgcaa ctgcactgct
      721 tgtttcggcc gtttgctcct ctgggacaga agtgcccgta gatggtgcca cttcttcaga
      781 agcaatcact gtctttgtaa cttctgaact tgatgctact tcttttggag cttctgcaat
      841 tggttgagaa aggtctgcaa cctgaacagt ttgatcatca acggtcactt gattagtggt
      901 caaatctgct gtcgcagttg tcacttcttc actagagtct gcttgaggtg tttggatttc
      961 aacttctgtt acttcttctg cttcattgac agtcgttgtc aaaacagttt ctgggaaaat
     1021 caagtccata ttagtgattt tgttcagatt cgcaagcact gtgacatcta cacccaaggc
     1081 ttctgcaatg gtgctcaaag tatcaccata ctgtacggta taacttgttt tgttgtccgt
     1141 tttagtcaaa tcgttttgga tttgctcaac actacgtgca gtccaaagaa cttcttctgc
     1201 ttgagttgcc aatactgggg caaatgacaa ggctactgtt gacgctaata acattctttt
     1261 cttcattctt tcaaattcct ttcaaatgag tacctgtcta tcataacaca aaaaatgtag
     1321 aaaaaaagcc ctctcgggcc tatttaacag aactgtctat tccttgtaac aaactagatt
     1381 acttgaaata gtttgttagg tctctgtcac aaaactgaca caatcttttt atcacttagt
     1441 ctcaaaaata cgggaaatat ctgctaacgc ttgaatcctg tgattccctt cataagtaga
     1501 ctctaaaaag ttgatacttt gaatcccact attctgggca aattccacat ccagagtccg
     1561 atcccctata taataagtat tatcagaatt caactgatac ttatctagca gataggtagc
     1621 cgcttctgga cttggcttcc gcacaaagcc actctgactg gtcaaaatct ctgtaaaata
     1681 ggattccacc cccaagtcct tgagaatggt aaaagtgttg ttccccttat gagtatatat
     1741 aaactgctga attcctgatt cgtctgccca agctagcacc tcacgcgcac ctggcatcaa
     1801 aactacctga gcattcttct cagccagact ctgggcacgc acctgattta gcacctcaac
     1861 atccagattt ctatcttctg ccacccgcac aagcaaattt tgcaccgaat acttgaagat
     1921 aaactctctc accttctcct tatcataagg aatagaaaac tgagcaaaag tctcctcaat
     1981 ccctgataaa atcgcttcgt aagagtccaa taaagtcccg tctaaatccc aaataaaagc
     2041 tgttttttgc atttcctatc ctttcaagct catatagcgc tggtaacgat agcgtagaaa
     2101 taaccagcga aaaccattat ccaagagaga ccctgcccaa ataccaggca agccccaacc
     2161 aagcacaata cccatcagat atcctgtccc aatgcggata caccacatgc ctatacttgt
     2221 cgcataaaaa gggaggcgtg catttcctaa tccctgccag actgccgtat agatgactgt
     2281 tcctgtcgtc ataggggtac caagtagtga aaacagtgtc actagaacac tagcctccac
     2341 cgctagagaa tcagtcgtat agagatgagt taatggtaca cccaagacat atatactaaa
     2401 ggacaggggc aacatgagga acagagaaag ccaaaaggtt tgtttactca aactagcaac
     2461 tcttttccaa tcatcctctc caactgctcg ggccaacagc atgaccgttg ccgtagcgac
     2521 accaaaggca ggcatatagt taaactgggt caagacttct ccgattgcat tcccagcaac
     2581 tgcctccgtc ccaaaagaaa cgaccaaggc aatgatcact acatctccag ccctcatcat
     2641 aagtcgctct ccagctgctg gtaaagccaa ggtcaacagt tccttatcta aaccaaaagt
     2701 tagcttccca taaggcagtt ttaattgtga ccacaaaatc acaagaccaa ccaaacgaga
     2761 cagaattgtc ccccaagcaa caccagctat ccccatatcc agaacaaaaa tagctagact
     2821 tgaaaaaaga atattcaagg cattggataa aaaactaaca tagagaggca gacgtggatt
     2881 atgcgttgca cgaatcaagg ctcccaaact agtcattaaa cctaagagaa caatcgatcc
     2941 gcctaccaaa gatagataca gtccaccact ctcagctaca tccctctccg tccccaaaag
     3001 tcctatcatc tctttcccag cgaagatgga caaaaatcct aaaaggaaac ttaatagtaa
     3061 ggtaatcttc aacgcctcag tcacatgata ggccaacttc gactggtctt tctgccctat
     3121 gctttttgaa ataacactgg aaatagcagc tcccagagcg atgaaaatcg cctgataaat
     3181 ggtgataata ttaccagcta ctgaaacccc tgaaatagct atcaatccta aatgagcaac
     3241 caaataactg tccaccattc ccattagcat ctgcaaaaag ttttcaccca tagctggcaa
     3301 tgcaatatta agaatgtctt tatttttctt aaacaatctc tcctcctgat gaaaagaaac
     3361 tcagttggtt tcccaaccga gtttgctccc tctgtcttaa agtcctaaat aagcctcaac
     3421 cgctgcttgc atgtcagcag ctgccactgt tgtcttgtga cgaattggag ctatttcaag
     3481 cccatcaact gctggtggca ctgcaacgcc tgagatttca tgtaattgag ccaaggcttc
     3541 aaagtctgtc aaacctgctt ttccagttac agcttctact gcaactactg ggaacttgta
     3601 tggactagct gttgaagcaa tcactgtctt agttacatct ccagtaaccg cttggtattt
     3661 tctataaact gctgaggcaa ccgccgtatg tggatcctca atataagaat ctgactcata
     3721 aacacgcttg atttctgccg ctgtttcttc ctcagtcgca tattcagctg caaagaggtc
     3781 caaaatctct gcatcaaagt ctgtcaactt atattgtcct tgcgtgttca aggcattcat
     3841 aagttcagct gtcttttcag cattatttcc caaaagatgg aaaatcaagc gctccaagtt
     3901 tgaagatacc aagatatcca tagatgggct ggttgttacc ttaaactcac gttttttgtc
     3961 atagacacgt gttttaaaga agtctgtcaa gacattattg tcatttgaag cacagattag
     4021 cttgccaact ggtaggccga tttgtttggc ataaaaggca gccaagatat ttccaaagtt
     4081 tcctgttggt actgtgaagt taaccttttc accagctaca atttcaccag tcttaaccaa
     4141 ttgagcgtaa gcataaacat aataaacaat ttgtggcacc agacgaccaa tgttcataga
     4201 gttagctgat gaaaattgca acttgttggt agtcaatttt tcacgaagag ccacgtcgtt
     4261 aaacatgtgc ttcacatttg tttgcgcatc gtcaaaatta ccatcaatag caataacatg
     4321 agtattgtca ccattctgag tggtcatttg caactcttgt accttgctga caccaccctt
     4381 tggataaaag acgataatct cagtaccagg cacatccgca aaccccgcca tagcagcttt
     4441 ccccgtgtca ccagatgtcg ctgtcaagat aacaatcttg ttctccaaac catgtttctt
     4501 agcagcagtc gtcataaagt atggcaaaat agacaaggcc atatccttaa aggcaatcgt
     4561 tgaaccatgg aaaagttcca aattgtattg cccatctaat ttcactaatg gtgcaatagc
     4621 tggagtatca aatttgctat cgtaggcatt gttgatacag tagtccaact cctcagctgt
     4681 aaagtcatct aaaaatgctg acaaaactag cttagcaact tcctggtaag aagcatcttt
     4741 caatttgtca aagtccaaat ctacctttgg ataagtaacc ggtgtaaaca aaccgccgtc
     4801 cgtcgccaaa ccttgcaaaa ttgcttggct ggcagttact gtattgttgg catcacgcgt
     4861 tgattgataa actaatgtca ttactctcta tcctttatct atagtctcgt ccattatatc
     4921 atgatttttc agaaaattct ccctttataa gagaaattat aggctcaatt cttctctcaa
     4981 aggctgtaaa taggtcattt cttccttatt tctcaccact agtccctcct cagctagtag
     5041 gagtaagtct ttggaaaatt caataatcgt agtttcttcc tcatctgtaa gatttttctt
     5101 agaaaattgt ctccttaaag acttgtaatc ataaccaaat actttaaaga aaggtgctgt
     5161 ttctaagtaa gcttctaact tgtctaaatt aaccaataat cccaagtgaa aagctgcaga
     5221 agcaaaagtc ctatcaagtg gctgtgtaca cacactacga aactcaactg ttcctcgagt
     5281 cgttaaatct tggtactggt aactacgatg agtttcaaaa tccttctctt gggggtaaat
     5341 aataacctca tccccattca gagcaaatgc ttggatttcg gacgtagcca aatagtcccc
     5401 agcctgaata ggataaaaat aataggtctg cccatcacgt tccgcagtaa aaatcgcaga
     5461 atgatttaga tagtcaaaaa aatcagcttc atcattaagg agtctagcat tgaccccaac
     5521 attctctgga tagataccat gcatagattc ttcccagaaa atatcccttg aaattttcgt
     5581 atcccaatcc gcacccgaaa attcagagtt tgcaaataaa taagccttag ccgcttcaat
     5641 ttgagtaaaa gcattaatca cccgtaagta gttggttttt gaaatatcca gctgaacctg
     5701 gctcccacag ataaaagtac catattcagg gaaatgatgt aaatctgatt taataatatt
     5761 tctactcaaa ttcaaataat ccatcaacat ctgatagcgt ggataagcca ctggacaatt
     5821 ctcattttta tcccagttgg gatggatacc acagccaaca atagcatgat ttgattcagc
     5881 taactttctc tgaattacat tcatataatt attgaaacga ttttcgacct cttgaatcgt
     5941 ttcagcctta ccaaatgcaa actcaatcgt tgtataggaa acttcaaata aaatagcatc
     6001 ctgacttatc ggatctacta actggatcag attgccaaaa tcatctacct ttgctacggt
     6061 gagatccaaa gtagaaacta aataatgaaa tagatccttt ataacttcaa catctgtagc
     6121 atccccttct aaacttgcaa caggatactc caactcaatt ccgacaaaca attcaggatt
     6181 ttctttaata tttttcaaat accgatgttt taataaatcg atagaacaag acatactacc
     6241 ctacactttc ttaatctaaa taaaatttga ataactataa ttataccaaa aataatataa
     6301 aaaaggaacg aagaataaca acgttcctaa cactatacta taccggcggc cggggtcgaa
     6361 ccggcacgtc cttgcggaca ctggattttg agtccagcgc gtctgccaat tccgccacgc
     6421 cggcaaatag taactggggt agctggattc gaaccaacgc atgagggagt caaagtccct
     6481 tgccttaccg cttggctata ccccaataat ataaaatagg cgagtgatgg ggatcgaacc
     6541 cacgcatgcc agagccacaa tctggtgtgt taaccacttc accacacccg ccataattct
     6601 attaacacgg gcagtaggaa ttgaacccac actgaaggtt ttggagacct tagttctacc
     6661 tttaaactat gcccgtaaaa tggaagggga gggattcgaa cccccgaacc cgaaggagcg
     6721 gatttacagt ccgccgcgtt tagcctcttc gctacccttc caaaatata
//
LOCUS       DAVBCN010000088         6752 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVBCN010000088 DAVBCN010000000
VERSION     DAVBCN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6752
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.088"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(119..1543)
                     /locus_tag="VQ497_001811"
     CDS             complement(119..1543)
                     /locus_tag="VQ497_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HET2518086.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMLKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2087..4366)
                     /gene="pulA"
                     /locus_tag="VQ497_001812"
     CDS             complement(2087..4366)
                     /gene="pulA"
                     /locus_tag="VQ497_001812"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HET2518087.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4478..6436)
                     /gene="ligA"
                     /locus_tag="VQ497_001813"
     CDS             complement(4478..6436)
                     /gene="ligA"
                     /locus_tag="VQ497_001813"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET2518088.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6529..>6752)
                     /locus_tag="VQ497_001814"
     CDS             complement(6529..>6752)
                     /locus_tag="VQ497_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2518089.1"
                     /translation="FFTQVQTKVESEFLGRVLSTIFTLAILFMPIAKGFMTVLPSVHL
                     SSFLIIGSGVIILSCISFIYVRTHFEKLI"
BASE COUNT         1984 a         1492 c         1227 g         2049 t
ORIGIN      
        1 atctgtacta ttctagtttc aatatactat aataccaaaa agttcgtatt ttttctataa
       61 ttattattat tttatagcaa aaaataaaac caggaaaaca atttcctgat tttacacgtt
      121 atttcacatc aaaaacaatg gatttgacat ttgtcatcgc ttcaatgcta tatttaattc
      181 cttgcactcc agctccagaa cctttgacac caaggaatgg gaaattatct ggaccacgct
      241 gggttttatt attaatgtgg actgtaccta cttcaagttt ttcagcaatt tcaaaggctt
      301 ttttgaaatc atttgtaaag actgatgatt gaaggccgaa ttcagattcg ttggcaaagg
      361 caatagcttc ctctacacta gccacacgaa tgattggtaa aacaggacca aatggctctt
      421 cccatgccac tttcatatct tttgtaactt ggtcaaaaag cactggccag aggagattgc
      481 cctcacgttt gattggtgta agagcctgag cctctttttc ttgtgcatcc tcaatcaagc
      541 cccaaatgaa gtcggctgaa gcattgtcaa taacaggtgt aatatcagca ttgtcaaatg
      601 gatcaccaac tgttaattta gaaacttctt cctgaagcaa agtagctaat ttatctgcta
      661 cactttcgag aacaatgaca cgtttaatgg ccgtgcaacg ttgtcctgag tagctaaagg
      721 ctcccgcaac aatttgcttg gcagcatgtt ccaaatctgc atcttctagt acaagagctg
      781 catctttccc accaagttcc aacatgatag gacgcatacc agctaaacga ccaatacgtt
      841 ctccaatagg agttgaacct gtaaagttga tgaagttgac ttctttgtgc tcaatgatat
      901 aatccccaat ttctgaacca cgacctgtaa tggtgttgaa aacacctgcc ggaatccctg
      961 cttcttcaaa tgctttagcc aacaagagtc cagaaatgga accttgtgtt ggtggcttca
     1021 acatgaccac attccctgca atcaaggcag gtgcaatttt agaagcagat aaattaactg
     1081 gataattaaa gggagcaatc gctagcacga taccaactgg ttcacgacgg acaacagcca
     1141 gtttgttttt acttgctgcc tcaaaaccac caccttccat tgcttgtcca gtgatacgga
     1201 gaccttcctc agcagcataa cgaatcaagt ctgctgtacg cactacttct ccaattgctg
     1261 ctttaatccc ttttgctact tctttggcaa ggatagtacc aattttttcc ttatcgcgtt
     1321 ctaaaatagc tgctgtttta tgcaaataag ccgcacgttc aattgctgat aaagctcgcc
     1381 atgctggcag ggctgcacgc gcagcttgca tagcctcatc agcttcagtc tgagtcatgg
     1441 ctggaactgt acccaattct tcttgattga ttggtgaata aatcgtaatt tcttgttcag
     1501 atgatttcca ttttccattt actaaattct gatatcttgt caaattcttg tcctcctgaa
     1561 aatgattaag atatatttta ccaaattttc agaaaataac aacccttttt gcaaaatttt
     1621 tgagattatt ttcacaagct tgatttttca aagtatactc aataaaaata aaaaaatcca
     1681 ctacgtcaag gcgaggctaa tgtggtttga agaaattttc gaagagcgtg aatgagtatc
     1741 atctatagta aaataaaaaa actgaacaat ttggttgggg acagccaaac caatttctca
     1801 caatgtttca gaaacaaggg tgtgctattc caatttcagc ctactataac tgtcatagat
     1861 tgctgaaaca aagtctaggt aaaagtcttc ataataaaaa gacctcctat caaatgttca
     1921 aaaactttga taggaggtct tgtttcgtga aaatatttat caaattttct atacaagtga
     1981 gctgttagcc aggttctttc tattctttca atttcaatga atggattttt taccaatact
     2041 cataactggg aatttttctg tctaaaaata gcgagataga tggtatttat aaaacactca
     2101 agacagctag actaatatca tttaaaacat tatcttcttt tgagcgactg ttggttacca
     2161 acatagctaa atttcctgca ttttcaaatt gatagggttc tgatttagca ttcacaacta
     2221 ccaagaggtg ttctttgccg tgaacttcat agataaggca gccgctatgt tcaatcgcag
     2281 aatgcacaaa gacatgatgg taaatttcat catagctaga gtaagaaaag gcaccagttt
     2341 ttgtcttcaa tcggatgact tgacggataa actcaatact gtcttgacgc tcattaatca
     2401 agttccagtt cacttggttc acactgtcag gagcattata gctattcatc gcacgctctc
     2461 tatcatcatg ggtcaactca ccattttcac cagtcgcaac cagtttggta cgaccaaatt
     2521 cttgaccgat ttccataaag gccatcccct gcatgagcag attcatggct gtggcagttt
     2581 cgaccttgcg catgatttgc tctgaacttt ggtctggatg aagggttgcc aataaatcgt
     2641 gaagattgta attgtcatgg gcttctacat agttaagcac ctgatttgga tgtgtatagc
     2701 ttcctaattc acgacttcct aggattgctt tagctagaat tggctctgtc gcagcaccac
     2761 tgacaaaacc tgacttgata gcaccataaa cttctccccc tttgacagca tcgcgctgat
     2821 tgtcattaaa gaaaccaata tttggcatct ggtaggcatt gtccttcttg gccttatcat
     2881 aaggggcaag acctgttccc atatcccatc cttctccata gaggataatg ttggagtcaa
     2941 tttcatccaa gctttgacga atcatctgca tggtcttgac atcatgaatc cccatcaagt
     3001 caaaacggaa gccgtcaata ttatattcct gcacccagta tagaagagaa tcaatcatat
     3061 acttgcgaaa catttcgtgt tcactggctg tttcatttcc aacacccgtt ccattctgga
     3121 aggtaccatc tggattcata cgataatagt aatcagggac tgttgtttgg aatggtgcat
     3181 caacaactga gaaggtatgg ttatagacta catccataat gactccaata cccgcatcgt
     3241 gataagcttg aaccatcacc ttcaaatcac gaatgacctg agctggatca tctggattag
     3301 ttgaaaaact agtttctggc gcgttatagt tttgtggatc ataaccccag ttgtaggtta
     3361 catttccatc ctcatcgtat tctttatgac ggtctgcaat tggttgcaat tgaacataat
     3421 tgtagcccag cttcttgatg taatcaaaag cagttgactg gccgtattgg ttaactgttc
     3481 cagcctgagc agcacccaag aaagttcctc gaagatgttc atctacaccc gatgtaggtg
     3541 atttagtcaa atcacgaata tgcatttcac agataactgc cttacatgga ttttccaagc
     3601 gccaagtagc ctccgaaccg tgcttaacct cgaagttttc aacttgcttt tctacatggc
     3661 tcagaatagc tgaacgtttg ccatcagggc tggtcgcgat tgtataagga tcacgtgtca
     3721 gtgtttggtg atgagggaat tggacttgat actgataagt cttacctacc aaatcttctt
     3781 caacatccaa actccagaca ccgattgtat tgtccttatg attataagag tagctattgc
     3841 ctcttttcat ctcaaaagtc ttccaaacgg gtgcatcatt agcagctgat tcataaacga
     3901 caacttgcac ttctgtcgct gtaggtgacc agagggaaaa atgagcctga ttgtcctcta
     3961 cacggcaacc caattctcct tggtaacccc aatgatgatc aaaactagca ctgttaatgg
     4021 ccttatcaaa ggcaaaagga ttttgatttt tatagaaagg actggcaata gcaggatttt
     4081 cagagtaata aatcctatca tcgccttcca aaatccagac ctctgttaat aggggatagt
     4141 gattaaaacg gatagaatat tctttactag tttgacctgt atgaaccaca aaattcaagc
     4201 tttctataac atgtgaactt gggtgttcaa agctaaataa agctccaaaa taatcttctt
     4261 tgtaggttag caaatcaatt cgttgatcct gactttttac aaaggagcaa gtgtcatatt
     4321 ctccattctt acgatggtaa tgaatgcgca tagggtagtt atacattttt tatttttcct
     4381 ttttactttg tttctatttc actaataaat ttttgtcaat ctcgtctcaa ttaacagaca
     4441 tagtcatatt ctctaaactc tgtttttaaa cgatccatta caaactttct agccatgcct
     4501 catctctgac ctggatacca agttcttgtg ctttttgcag tttacttcca gcgtctgcac
     4561 ctaccacgac gaggtcggtc tttttagaaa cactacctgt cactttggca cccagacttt
     4621 cgagtttact tttagcttct gagcgcttga gtcgttccaa ttttcctgtc aataccacgg
     4681 tcaaacctga caaggccgca tccgctacta ccgtctgtcc tttatagtcc agattgaccc
     4741 cagtttcttt caattctctg agcagaattt cagagccttc tgtcgcaaaa taagtctgaa
     4801 gacttttggc aatcacgcca cctagacttt caatactagc cacttcctct gaatctgcct
     4861 gagacagatt ttcaattgaa tggaaatatt gaagtaaaag ctgactagcc ttgcttccga
     4921 catgacgaat tcccaaacca aataagagct tctcggcaga attttccttt gatgcttgga
     4981 tagcctgata cagtttagca gcggactttt ccttaacccc ctctaaaagg aggaaatcct
     5041 cttcttgcaa acgataaata tccgccacat ccttgactaa attagcagca aaaagcttct
     5101 caacaataga tggaccaagg cctgtaatat tcatagcatc acgagaagca aagtgaatca
     5161 agccttccat gatttgagca gggcaacgcg gattgataca acgtagggcc acttcatctt
     5221 caaagtgcaa caagtcagag ttacaacttg gacagtttgt agggatatct agtttttctt
     5281 cagaaacccg tttggactct accacacgta aaacggcagg gatgatgtca ccagccttat
     5341 atacaatgac cgtatcgtct tttcggatat ctttttcagc aatataatct acattgtgca
     5401 gggtcgcacg gctaacagtc gtaccggcaa gttgtactgg tgttagatta gcagttggag
     5461 ttacaacacc ggtacggcca actgtccagt caactgataa gagttgagct tctttttctt
     5521 cagcagggaa cttgtaggct actgcccact ttggagcctt aacggtaaaa ccaagttctt
     5581 cttgacttgc taggtcgttg accttgatta ccactccatc aatatcgtaa ggcagatttt
     5641 cccgttcctg tcctacttct tggataaaat tccagatttc atctatgttt tcagccaaga
     5701 ttcgcttagg attgaccaca aaacctagtt gttctaggta cttcaaaccc ttttcttggc
     5761 tatcacgagt tgaagggctg gcttcttgat agagaaacgt tgcaagatta cgcttggcaa
     5821 ctactgctgt atccaactga cgcagagttc ctgctgccgc attacgagga ttagcaaatt
     5881 caggctctcc attttcttgg cgcacttggt taacttggtc aaaggaagcg cgtggcatgt
     5941 aacattcccc acgaactgtg atatctagtt cttctggcaa agtcaaaggg atgtccttaa
     6001 cacgcttgag gttttctgtg atattttcac caattgaacc atctccacgt gttaccccag
     6061 caaccaaaat ccccttttca taagtcagcg agatagataa gccatcgatt ttcagctcac
     6121 aaatataggt cggatgagcc acttccttac gaacacgcgc atcaaaagca tctagctcct
     6181 cacgtgaaaa agcatcctgc aaactataaa gaggatactg atgactgtat ttttcaaaac
     6241 catctaaaac cttgccacca acacgatgag tcggactgtc tgctagcact tgctctggat
     6301 aagcagtttc taactcgacc aactcacggt aaaggcggtc atactcactg tctgaaaccg
     6361 agggattatc gctggtatag tactcagtcg catagcgatt gagcaaagcg actaactcat
     6421 tcattctttt attcataaga ccattttacc ataaaacaag ccctcctcac aaacgagaag
     6481 ggcggaaaaa acacttagtt tgaaattatt tttgaaactc aagcaacctt atatcaattt
     6541 ttcaaaatga gttcgaacat aaataaacga tatacaagac aagatgataa caccacttcc
     6601 aattatcagg aaagaagaga gatgtacact tggcaagact gtcataaatc cttttgcaat
     6661 aggcataaat agaatagcta aggtaaaaat tgtactcagt actcttccaa gaaattcgct
     6721 ctcaaccttg gtttgtactt gagtaaaaaa gt
//
LOCUS       DAVBCN010000089         6693 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVBCN010000089 DAVBCN010000000
VERSION     DAVBCN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6693
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.089"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            244..1110
                     /locus_tag="VQ497_001815"
     CDS             244..1110
                     /locus_tag="VQ497_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2518090.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLHALAKKLKVSMDYFFDEQVVEKVEELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKIYMEWIKSLIDFYFYGQKEDAIVRLEGVLSQLSVSDLNYLQVSNTLFN
                     FYYDIGDLGRFNKIREKLEYQVNHLNLSTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKAYRTNYLLADLYLLMGNVSENFSSKSSVKEYFETAYFLYKLEENMSMT
                     LKVEHYIADITE"
     gene            1447..2004
                     /locus_tag="VQ497_001816"
     CDS             1447..2004
                     /locus_tag="VQ497_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase family
                     flavoprotein"
                     /protein_id="HET2518091.1"
                     /translation="MDKKNLLVLVTGSVGASNVDTYLYYIKKFYNVKVILSENSKKFI
                     SKELISYFCDKVYDEIFVEEVVPHVFLPYESDLLLILPATANVIGKIANGIADDLVTA
                     TVLNFNKKIIFCPNMNSTMWDNHIVQRNVSILKELGHIFLFESKKTYEVGLRKAIDST
                     CSMLQPQSLVKELIKLENIVLEEGH"
     gene            2225..3331
                     /locus_tag="VQ497_001817"
     CDS             2225..3331
                     /locus_tag="VQ497_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET2518092.1"
                     /translation="MINKKIQQVVLESLQNFLNGNFISPCVVYDFGLLETVLDEFKNQ
                     IPVTFNYQLFYAVKANSNEKILEFLVDKIDGVDVASLSELDVAKKFFPPTQISVNGPA
                     FSYETLYNLIKKQYKVDINFLEHLQQFSPKESVGIRVTEPDELNNRMSRFGINICSDN
                     WTSNLQNPLITRLHFHFGEKDDKFIVKLDKILFKLQEINKLREVREINLGGGFMKLFM
                     ENRLKEFFLSLMEIYKKYDIDSTVTTIIEPGSAITSFSAYMITSPVNVSEVNEQQVIT
                     LDTSIYTNTLWFVPHIITTLNSSSKERYSTILYGNTCYEHDKYKMKVSLPRLTQNSSI
                     VFFPVGAYIKSNHSNLHRNDFMREVYLWTKNLTY"
     gene            3307..4698
                     /locus_tag="VQ497_001818"
     CDS             3307..4698
                     /locus_tag="VQ497_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518093.1"
                     /translation="MDKKLDILDKVKEYLGNKTTQILDNQYKEFLKLNDIRRAFGISE
                     KVLNNSFNFTSKEFNDLINNENYLFEYACRIREEWRKKCFNHSYRFLCSPIITDDFLN
                     TKTLRSSQIEYKYERYLSKSSIGDRAVDGFVSFNTLTANGMSAIKLCLEILNSIFFKK
                     KIDLLYSTGYYETRFLLNNLAKSGISCYEVSNCELDKDKFYNVFMMEPNRADLTLQKT
                     DFKIVEYFVKYKNNSIKVVILDISYQGSNFKLVEFLEKFKFANVIIFVVRSLIKLDQM
                     GLELTNGGIIEVFIPNHLRKLKNFIEEEFNKFRNSHGANLSLYEYCLLDNSLTLKNDW
                     NYSDLVMKFTSNFYADIKDLFMENSDIEIIHEEGVPFVFLDLIGEGKKEYEMFFQWLN
                     FFYKQLGITLYARNSFGFRNLTVEYFGIIGTERYIFKICPGVYKGLSYYLMKFLLKSF
                     SNEYLKTTDEVNR"
     gene            4695..5873
                     /locus_tag="VQ497_001819"
     CDS             4695..5873
                     /locus_tag="VQ497_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2518094.1"
                     /translation="MKNLIKLLIIRLIVNLADSVFYIVALWHVSNNYSSSMFLGIFIA
                     VNYLPDLLLIFFGPVIDRVNPQKILIISILVQLAVAVIFLLLLNQISFWVIMSLVFIS
                     VMASSISYVIEDVLIPQVVEYDKIVFANSLFSISYKVLDSIFNSFASFLQVAVGFILL
                     VKIDIGIFLLALFILLLLKFRTSNANIENFSFKYYKREVLQGTKFILNNKLLFKTSIS
                     LTLINFFYSFQTVVVPIFSIRYFDGPIFYGIFLTIAGLGGILGNMLAPIVIKYLKSNQ
                     IVGVFLFLNGSSWLVAIVIKDYTLSLILFFVCFMSKGVFNIIFNSLYQQIPPHQLLGR
                     VNTTIDSIISFGMPIGSLVAGTLIDLNIELVLIAISIPYFLFSYIFYTDNGLKEFSIY
                     "
     gene            6298..6531
                     /locus_tag="VQ497_001820"
     CDS             6298..6531
                     /locus_tag="VQ497_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2518095.1"
                     /translation="MISIDLGGTNIKITVLSNDGEIETLWSITTDTSEKGSQIISDII
                     SSIKNKLTERNIPDSDLLGIGMGSCSSYFPCKS"
BASE COUNT         2355 a          789 c         1197 g         2352 t
ORIGIN      
        1 gaaaggggct gaaagaacta ggggaacagt tgtaattaga aggtgatagg tatgaaaagt
       61 ggcgttgaag ctggataagt ttataaagag tttgaatgaa gaaatgaaaa gtaaataagt
      121 tgatgatgaa ttgcaaaaat agattttata tacaatttgt atctttcaaa tcattgattt
      181 gaattttaga tttaatcatg ttacaattaa aaatgaaatc aaatagaaga ggaagatgca
      241 aacgtgggca cactattagc aacaagatta aaaaatagac gaaaagaatt aaaaatgtct
      301 cagcgagaac tagctgaagg tatctgtaaa caggggcaaa tcagtcgatt agagagcgga
      361 gagtttactc ctggagcaga tttcttgcat gcccttgcta agaagttaaa agtcagtatg
      421 gattattttt ttgatgagca ggttgtagag aaagttgagg agttatcaga atttaagaaa
      481 ttagcacaga catttattac aaatcgcaat tatgagtctt tgaaatatat atatgaattg
      541 gagaatgtaa aagctcaccg cctatcttta gcagataaaa tctacatgga atggataaaa
      601 tctctgatag atttttattt ttacggacag aaagaagatg cgattgtacg attagagggg
      661 gtattatcac agttaagtgt atctgatttg aactatctcc aagtttcaaa caccctattc
      721 aatttttatt atgatattgg agatttaggt cgtttcaata aaattcgtga aaaactagag
      781 tatcaagtaa atcacctcaa tttgagtaca atcgaagagt tagaactatc tattaagttt
      841 aactacaacg tttgtcgtta tctatggtta cagaaaaata ttgaagaagc tattactaaa
      901 attacagcta ctataaaaca atgtaaggct tacagaacga attatctttt ggctgatttg
      961 tacttattga tgggaaatgt aagtgaaaat ttttcttcta aaagttcggt gaaagaatat
     1021 tttgagacgg catattttct atacaaactt gaggagaaca tgtcaatgac tctgaaagtt
     1081 gagcattaca ttgcagatat aacagaataa tgaatacggt gttcaagtga gcatcgtttt
     1141 tcaaacctag ttcagataca aaaaagtatc aaataaaaaa aaataaaaaa acattataaa
     1201 aacaattgac aagctaattt tgaagtgata aaataaatgc aagaaaacaa aaggagaggt
     1261 atatgagaag tttgtttaag aaaatagttg ctgtattagt tattggctta attctgttag
     1321 gaattgcgag aacacctcaa gttcataaga tggctagagg gattgatcct ggtcctgcga
     1381 attttattta gaaatcaatt tctactaatt gagtactaat tgagtattaa tgggtaaaaa
     1441 gattgtatgg ataagaaaaa tttacttgta ttggttactg gttcggtagg tgccagtaat
     1501 gtagatacat acttatatta tataaaaaaa ttctataatg ttaaagtgat actttctgaa
     1561 aattctaaaa aatttataag taaggaatta attagttatt tttgtgataa agtttatgat
     1621 gaaatatttg ttgaagaggt agttccgcac gtttttctgc catatgaatc tgacttactt
     1681 cttattttac cagctacggc aaatgtgatt ggcaaaattg ctaatggtat tgctgatgat
     1741 ttagttacag caactgtttt aaactttaat aaaaaaataa ttttttgtcc caatatgaac
     1801 tctactatgt gggacaatca catagttcaa agaaatgtat caattctaaa ggagttggga
     1861 catatatttt tatttgagtc taaaaaaaca tatgaggtag gattgcgtaa agcaatagat
     1921 tcaacatgtt caatgttaca accacagtcg ttagtaaaag aacttatcaa attagaaaat
     1981 attgtccttg aagagggaca ttaaaaacta ctgagaatat taaggagggg aaaaaatgga
     2041 aaattcatca atcgatgtag atatgctgtt ggaagaattg acacaagaag caatggtcgt
     2101 tgttgctgtt gataaggact gttaatttaa acttatggca atatatgaaa ggttactgga
     2161 tgttttaaat tatgcaggca gtagcctttt attatataca aatggataaa gtaaggataa
     2221 tacaatgatt aataaaaaaa tacaacaagt tgttttggaa tcattacaga attttttgaa
     2281 tgggaacttc atttcgcctt gtgtagtcta tgattttggc ttgctggaaa ctgtacttga
     2341 tgaatttaaa aatcaaattc ctgtaacatt caattaccaa cttttttatg ccgttaaagc
     2401 aaattcaaat gagaagatac ttgaattctt agtagataaa attgatggag ttgatgtggc
     2461 gtcattatct gaattagatg tggctaaaaa atttttccca ccaactcaaa tttctgttaa
     2521 tggtcccgca ttttcttatg aaactttata taatctgatt aaaaaacaat ataaagttga
     2581 tattaacttt ttggaacatc ttcaacaatt ttccccaaaa gaatctgttg gaataagagt
     2641 aacggagcca gatgaactta ataatcgtat gagtcgattt ggaataaata tttgcagtga
     2701 taattggact agtaatttac aaaatccttt aattacacga ctgcattttc attttggaga
     2761 aaaagatgat aaatttattg ttaagttaga taaaatatta tttaagttac aagaaattaa
     2821 taaacttaga gaggttagag aaataaatct tggaggcggt tttatgaaat tatttatgga
     2881 aaatcgtttg aaagaatttt ttctatcact tatggaaatc tataaaaagt acgatattga
     2941 tagtactgtg actacaataa tagaaccagg tagtgcaatt acttcatttt ctgcctatat
     3001 gattactagc ccagttaatg ttagtgaggt gaatgagcag caggttatca cgttagacac
     3061 atcaatatac accaatacat tatggtttgt tccgcatatt attacaacgt taaattcaag
     3121 tagtaaagag cgttatagta ctattctcta tggtaatacc tgttatgaac atgacaagta
     3181 taaaatgaaa gtttcgcttc caaggttaac tcaaaatagc agtatagtgt tttttcctgt
     3241 aggagcttat ataaaaagca atcattcaaa tttacatcgt aatgatttta tgcgggaggt
     3301 atatttgtgg acaaaaaact tgacatatta gataaagtta aggaatattt aggaaataaa
     3361 actactcaaa ttctggataa tcaatataaa gaatttttga aacttaatga tataaggcga
     3421 gcgtttggta tttcagaaaa agtattaaac aattctttta attttacgag taaagaattt
     3481 aatgatttaa ttaataacga aaattattta ttcgaatatg catgtagaat tagagaggaa
     3541 tggagaaaaa aatgctttaa tcattcttat cgttttctat gctcacctat aattacagat
     3601 gattttctta acacgaagac attgagaagt agccaaattg aatataaata tgagcgatat
     3661 ttatcgaaaa gttcgatagg cgatagagcg gttgatggct ttgtttcctt caatacttta
     3721 acagctaatg gtatgtctgc tattaaacta tgtcttgaga tattaaactc tattttcttc
     3781 aagaagaaga ttgatttatt atattcaacc ggatattatg aaacaagatt tttattaaat
     3841 aatcttgcta aatcaggtat tagttgctat gaggtaagta attgtgaatt ggataaagat
     3901 aaattttata atgtattcat gatggaaccc aatcgagccg atttaacatt acaaaaaact
     3961 gatttcaaga tagtagaata ttttgttaag tataaaaata attcaataaa agtcgttatt
     4021 ttagatattt catatcaagg ttctaatttt aaattagtag aatttttaga gaaatttaaa
     4081 tttgcgaatg taattatttt tgtggtacga tctttgataa aattagatca aatgggatta
     4141 gaattgacaa atgggggaat aatagaagtg tttattccta atcatttgag aaagttgaaa
     4201 aattttattg aagaggaatt caataaattt agaaattctc acggagctaa tctaagcctc
     4261 tatgaatact gtttgcttga taattcttta accttaaaaa atgattggaa ctattctgat
     4321 ttagttatga aatttacgag taatttttat gctgatataa aagacttgtt catggaaaat
     4381 tctgatattg aaatcatcca tgaagaggga gtaccttttg tatttttaga tttaataggt
     4441 gaaggtaaaa aagaatatga aatgtttttt caatggttaa acttctttta caaacagctt
     4501 ggaatcacat tgtatgctag aaatagtttt gggtttcgga atctaacagt agagtatttt
     4561 ggaattattg ggacagaaag atatatattt aagatttgtc caggtgttta taaagggtta
     4621 agttattatt tgatgaaatt tttattaaaa tctttttcaa atgaatattt aaaaactact
     4681 gatgaggtta atagatgaaa aatttgataa agttgctaat aattagattg attgttaact
     4741 tagcagacag tgtattttat atagtagcat tgtggcacgt tagcaataat tattcttcga
     4801 gcatgttctt aggaatattt attgcagtaa attatctacc ggatttgtta ctaatctttt
     4861 ttggaccagt tattgacaga gtaaatccgc aaaaaattct tataatatca attttggttc
     4921 aattagcagt ggctgtaata tttttattat tattaaacca aatatcattt tgggtgataa
     4981 tgagtctagt gtttatttca gtaatggcta gctccataag ttacgtgata gaagatgtgt
     5041 tgattcctca agtggtagaa tatgataaga ttgtatttgc aaattctctt tttagtattt
     5101 cgtataaagt attagattct atttttaatt cattcgcatc atttttacag gtggcagtag
     5161 gatttatttt attggttaag atagatatag gcatattttt acttgctcta tttatattgt
     5221 tgttgttaaa atttagaact agcaatgcga atatagaaaa cttctctttc aaatattaca
     5281 agagagaagt gttgcaaggt acaaagttta ttttaaataa taaattatta tttaaaacca
     5341 gtatttcttt aacgcttata aacttttttt attcatttca gacagtagtt gtaccgattt
     5401 tttctattcg atattttgat ggtccgattt tttatggtat ttttttaact attgctggtt
     5461 tgggtggtat attgggaaat atgctagcgc caatcgtaat aaaatattta aaatcgaatc
     5521 aaattgttgg tgtatttctt tttttgaacg gctcaagttg gttagtagca attgttataa
     5581 aagactatac tttatcactt attttatttt tcgtttgttt tatgtctaaa ggagtcttca
     5641 atattatttt taattcgttg taccaacaaa tacctccaca tcaacttctt ggtagggtaa
     5701 atactaccat tgattctatt atttcttttg gaatgccaat tggtagttta gttgcaggaa
     5761 cgcttattga tttgaatatt gaattagtgt taattgctat tagcatacct tattttttgt
     5821 tttcttatat tttttatacg gataatggat tgaaagaatt tagtatatat tagaaatgtt
     5881 tatgttcatt caaaagcata atgactataa ctgaaaaaga aaagtgatat ctttaaggtt
     5941 gttcttcttg gtggtgagat tcgtgagaca acccaagctt ttgtcggaaa gattaccaat
     6001 gctttgatgg ataggatgta ctttagcaag atgtttttag tggtaacggt atcgtggatg
     6061 gacgtgtaat aacctcttct ttcgaggagt attttactaa aaaactagcc ttggagcgtt
     6121 ccccagaaac ggacttactc attgactctt caaagatttg gggagaagat tttgcttcat
     6181 ctgttccttg aaaaaagtca cagcagtcat cacagacgat agtactgaac aaaactatga
     6241 agagttagaa atttatacgc aggtgattgt ataaaggatc tggaaataga taagaagttg
     6301 attagtattg acctaggtgg tacaaatatt aagattactg ttctttcaaa tgacggtgag
     6361 attgaaactt tgtggagtat tacaacagat acaagtgaga aaggttctca aattatatcg
     6421 gacatcatca gttctattaa aaataaattg accgaacgga atattcctga tagcgacctt
     6481 cttggaatcg gtatgggaag ttgctcatca tactttcctt gtaaatcata ggggctataa
     6541 actctccgtc tacttgtcct gcaacaattg aagtctgctc aaaacgccgt ccgctaatct
     6601 tttcatagac tttctccctt ttaggagcct agctttctag tttgttcttt gatttttatt
     6661 gagtatacca ctattttact ccctctggca agg
//
LOCUS       DAVBCN010000090         6637 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVBCN010000090 DAVBCN010000000
VERSION     DAVBCN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.090"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(<1..189)
                     /locus_tag="VQ497_001821"
     CDS             complement(<1..189)
                     /locus_tag="VQ497_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET2518096.1"
                     /translation="MEHIILLRGVTPNGKNAIPKMSYLVDILTEADFQQVRTYIQSGN
                     IILESNLALEEIREQVHTL"
     gene            complement(240..1412)
                     /locus_tag="VQ497_001822"
     CDS             complement(240..1412)
                     /locus_tag="VQ497_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET2518097.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSNPI"
     gene            complement(1471..2316)
                     /locus_tag="VQ497_001823"
     CDS             complement(1471..2316)
                     /locus_tag="VQ497_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2518098.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEDGGLLM
                     GYEID"
     gene            2441..2998
                     /locus_tag="VQ497_001824"
     CDS             2441..2998
                     /locus_tag="VQ497_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET2518099.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            3017..3484
                     /locus_tag="VQ497_001825"
     CDS             3017..3484
                     /locus_tag="VQ497_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HET2518100.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            3573..4202
                     /gene="upp"
                     /locus_tag="VQ497_001826"
     CDS             3573..4202
                     /gene="upp"
                     /locus_tag="VQ497_001826"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET2518101.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETFQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            4376..4966
                     /gene="clpP"
                     /locus_tag="VQ497_001827"
     CDS             4376..4966
                     /gene="clpP"
                     /locus_tag="VQ497_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HET2518102.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            5045..5293
                     /locus_tag="VQ497_001828"
     CDS             5045..5293
                     /locus_tag="VQ497_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HET2518103.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            5394..6554
                     /locus_tag="VQ497_001829"
     CDS             5394..6554
                     /locus_tag="VQ497_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2518104.1"
                     /translation="MKKKFALSFVALASVALLAACVEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTNMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
BASE COUNT         2071 a         1287 c         1386 g         1893 t
ORIGIN      
        1 tagagtatga acctgttctc gtatttcttc gagagctaag ttactttcaa gaataatatt
       61 tccactttga atataagttc gaacttgttg aaaatcagct tctgtcaaga tatctactag
      121 ataagacatt ttagggatag cattttttcc attaggagtg actcccctta gcaagataat
      181 atgttccaaa gtagttcctt tctttttttg gggggattgt tccatttagt aatgtatatt
      241 tagattggat ttgatcccgc cacccagccg gtacagaggg cagaagtgat gttaaagcca
      301 cctgtgtggg cattgatatc cataacttcg cctgcaaagt ggaggccagg taccagctta
      361 ctttcaaggg ttttaggatt gatttccttg agactgactc cacccttggt aacaaaggac
      421 tttgcaaggg acatttttcc agttacagga attttaagtt ctttaatgga ctggacaagt
      481 tgttctcgtt ccttttcagt cagttgtttg actttttcag gatatccttg tacaaaaaat
      541 tcggccaagc gttctggtaa caaggttttt aaagcgtttt tcaaggattt ttcccgattt
      601 tcttctagaa atgtaaccaa gtccttctca gaaagttgag gcaaaacatc gagtgagaga
      661 acctccccac ctttgacaaa gctagacatg cgtagggcag caggacctga caaaccaaag
      721 tgggtaaaga gtaaatcatg agtgatgaca tgcttaccat aacttagggt cacatcgtcc
      781 aaagaaatcc cttgtaaggc tttatgtgga aaatctgtta ataaaggact ttcagcagcc
      841 tcaagatcgg tgatggtatg cttaaaatgg cgagcaatct cgtgaccaaa accagtcgaa
      901 ccagtcgaag gataagactt accacctgtt gtgacaatga gtttctcaca agtgaaggtt
      961 tgatccgctg acttaaggac aaactggtca tctacttttt taacagaaac gatttctatt
     1021 tgagtagcaa cttgaccacc tagttcggtg attttctttt ccaaagcttc gataatagtc
     1081 cgagacttgt cactggctgg aaagacgcgt ccgtggtctt cgaccttaag tttaacacca
     1141 ttttctgtaa aaaagttgat gatgtcatga ttatcgaact gggagaaaac actgtaaaga
     1201 aagcgtccgt ttccaggaat tccagctagc aggttgtcta agctaccatt gttggtcaca
     1261 ttgcaacgtc ccccaccagt cccagctaat ttttttccaa gtttccgatt tttttcgatg
     1321 aggagggttt tctgtccata aaagctactg gaaatcgtag ccatcatacc agcaggtccc
     1381 ccaccgatga caatagtatc aaaatgtttc atagctctat tgtaccacaa aaaaacaaga
     1441 gatgatggtc acctcttgtc aagaatacaa ttaatcaatt tcatagccca tcagcaaacc
     1501 gccatcttct gcatagaaac tgcagagacc agaggttggt agaattttaa tatccgcttg
     1561 tgggaaggtt tcacggattc gctctgagag ctgttgacaa catttttcgt tattgcgttg
     1621 ggccatgaca atacggccac cagcatatcc agcttttact aactcatcat aggcagcttg
     1681 aactgatttc tttgatcccc ttgctttttg tagcaattcg agagttccag tttcactagc
     1741 ttttccgacc atacgaatgt tgagaaggcc aacgaccgta ccgataagct tgctcaaacg
     1801 gccgttcttc accaagttat cgactttggc taggacaaag agcaacttag ttttttcttg
     1861 ataggcggtg atagcttcaa ccacttcttc aaaagacaag ccctggtcaa tcaagtcatt
     1921 caatttttct acgagtaggt caacttcacc accagcagat aaactatcaa tcacatgaat
     1981 cttagtgtca ggatggtctt ccagataaat attctttgct agttgagcac tattgtgact
     2041 gccagaaagg gtacctgtga tggttactag gaaaatgttt ttggcacctt caaatgctcg
     2101 caaatagtca tctgggcttg gacaagccga ttttgaagct tctgcagttg catacatggt
     2161 ttccatcatt tggtcaatat cgagactggc gtcatcaaca aagacctgat cagctacttg
     2221 aatggttaag gggacactta caaaggttgt gttaatagct ggtgttggca gttgacgata
     2281 atcacaacca gagtcagcaa taatcttcca agtcatagaa attctccatc tttgtcagtt
     2341 atacattgac aaaggttctg tcttttttta caattatatc atgaaaaccc ttgaaacaaa
     2401 agcatcatcc gtctgttgtg acaagtagaa agaaaatgtt atgtctgaac gtagaatctc
     2461 tgaaaagtct cttgaaaatc tcagaaaatc aaaccaagaa tccaatttat taaccagaga
     2521 agccattgaa acagccctct tgcaactctt ggaaaaaaag gaactgacca agattagtat
     2581 ttctgaattg gtcaaacgtg caggtgtttc gcgtgcggcc ttttatcgca attatgactc
     2641 caaagaggaa attttagaga gcgtctttaa acgaactgtc cacaatatta tggaacagct
     2701 gcatcattac gatttaaaga cagaccttta tttggtttgg gttcaccttt tccgggaggc
     2761 cagaaaggaa gctagagtaa ttcaattggc cttggattac catctggaaa aaatctttgt
     2821 ccaagccatg caggaatttc tagaaaaata ccatgggaaa tcaaaaggtg tcagctctta
     2881 tcttcattcc ttctggagct cagccatcgt ctctgtcctt ctaaaatgga tcaaggatgg
     2941 catgaaggta ccagctgaaa agattgcaga tttaggttta ccatttttta aaaaatagag
     3001 aaaaaggaga aaagagatga ctgaaaaaag actggcctgg gatgagtatt ttgcagccca
     3061 agctctacta attgcgaatc gttccacttg taaacgtgct aaagtgggcg cgattctggt
     3121 aaaagataat aaggttattt ccactggtta caatggttcg gtgtcaggga ctgagcattg
     3181 tattgatcac gaatgtttgg tcattgaagg ccactgtgtt cgcacccttc acgctgaggt
     3241 caatgctatc cttcaaggtg ctgagcgtgg tgttcccaaa ggctttacag cctatgtaac
     3301 ccattttcct tgtctcaact gtacaaaaca attgcttcag gtcggctgca agcgtgtggt
     3361 ttatatcaac cagtaccgaa tggacgacta cgcccaatac ctttatcaag aaaaagggac
     3421 agaattgacc catttaccac ttgagacagt acagacagct cttaaagagg ctgatctaat
     3481 gtaaaaatta tcaaaaataa atggtttaga aagattttta acccgttttt tggtataata
     3541 agaagaataa attgaaagaa ggaattccaa aaatgggaaa aattgaagtt attaatcacc
     3601 cactgattca acacaaattg tcaatcttgc gtcgtacaga tacttctaca aaagcttttc
     3661 gtgagctagt agatgagatt gcaatgttga tggggtatga agtacttcgt gatcttccac
     3721 tagaagatgt ggaaatcgaa acaccaatta caaaaacagt tcaaaaacaa ttggcaggta
     3781 agaaattggc catcgtccca atcttgcgtg caggtatcgg gatggttgat ggtctcttga
     3841 gcttggttcc agctgctaaa gttggccaca tcggtatgta ccgtgatgaa gaaacatttc
     3901 aaccagttga atacttggtg aaattgcctg aggacattga ccaacgtcaa atttttgtag
     3961 tagacccaat gttggcaaca ggtggctcag caatcttggc tgttgattct cttaaaaaac
     4021 gtggcgcatc aaatatcaaa tttgtctgcc ttgtatctgc tccagagggt gtaaaagccc
     4081 ttcaagaagc tcatccagat gtagaaatct ttacagcagc cttggatgaa cgtttgaacg
     4141 aacacggtta tatcgttcca ggtcttggag atgctggaga ccgcttgttc ggtacaaaat
     4201 aagatcgaaa agaagaatga cttggaaaag tatttccagt cacgaaagga ggttgggttt
     4261 ttgtttctgt ctaatgaaag cagagcaaaa atttgacctt ttttgaccaa gatattataa
     4321 tagtcttatc ttcagtcatt tgaccaacaa aattaaaact caaaaggaga aatgaatgat
     4381 tcctgtagtt attgaacaaa caagccgtgg agaacgttct tacgatattt actcacgtct
     4441 tctcaaagac cgcatcatta tgctgacagg tccggttgaa gacaatatgg ctaactctgt
     4501 tattgcccaa ctgcttttct tggatgccca agatagtaca aaagatattt acctttatgt
     4561 caatacacca ggtggttctg tttcagctgg tttggcaatc gtagatacca tgaactttat
     4621 caaggcagat gtccaaacca ttgttatggg aatggctgca tctatgggaa cagtcatcgc
     4681 atcaagtgga gcaaaaggca aacgtttcat gcttccaaat gctgaataca tgattcacca
     4741 accaatgggc ggtacaggtg gtggtaccca acaaactgat atggctatcg ctgcagaaca
     4801 cttgctcaaa actcgtaata ccttggaaaa aatcttggct gaaaattcag gtcagtcaat
     4861 ggaaaaagtc catgcagatg cagaacgtga taactggatg agcgcccagg aaacacttga
     4921 atatggcttt attgatgaaa ttatggccaa caattcattg aactaatgat gatagaaggc
     4981 aaactcgact gggtttgctt tttttggtat aatagggaga gatttcttag aaagaggatt
     5041 tatcatgttt gaaaaagtca atcgctctgg cttgattatc tatctttact ataatcgtga
     5101 tgccaaaaaa ctgcaggatt atggagatat tacctatcat tccaagaaac atcgttactt
     5161 acaactctat gttccaactc aagaagtgga gcaattggtc ggacgcttga gcaaggaaaa
     5221 atttattaaa aaagttaggg tttgtcatat ccaagagttg gaaacaccct ttgtgggcaa
     5281 tctttatcga taggaaaacg ttatcatcga aaaagttcaa ggaaaacatt gacaattttc
     5341 tgataattcg gtatattctt aacagactat ttaagaaata aggagacaaa aagatgaaga
     5401 aaaaatttgc cctatcgttt gtggcgcttg caagtgtagc acttcttgca gcctgtgtag
     5461 aagtgaagtc tggagcagtc aacactgctg gtaactcagt agaggaaaag acaattaaaa
     5521 tcgggtttaa ctttgaagaa tcaggttctt tagctgcata cggaacagct gaacaaaaag
     5581 gtgcccaatt ggctgttgat gaaatcaatg ccgcaggtgg tatcgatgga aaacaaatcg
     5641 aagtagtcga taaagataat aagtctgaaa cagctgaggc tgcttcagtt acaactaacc
     5701 ttgtaaccca atctaaagta tcagcagtcg taggacctgc gacatctggt gcgactgcag
     5761 ctgcggtagc gaacgctaca aaagcaggtg ttccattgat ctcaccaagt gcgactcaag
     5821 atggattgac taaaggtcaa gattacctct ttattggaac tttccaagat agcttccaag
     5881 gaaaaattat ctcaaactat gtttctgaaa aattaaatgc taagaaagtt gttctttaca
     5941 ctgacaatgc cagtgactat gctaaaggga ttgctaaatc tttccgcgag tcatacaagg
     6001 gtgaaatcgt tgcagatgaa actttcgtag caggtgacac agacttccaa gcagccctta
     6061 caaacatgaa agggaaagac tttgatgcta tcgttgttcc tggttactat aatgaggctg
     6121 gtaagattgt aaaccaagcg cgtggcatgg gaattgacaa accaatcgtt ggtggtgatg
     6181 gattcaacgg tgaggagttt gtacaacaag caactgctga aaaagcatca aacatctact
     6241 ttatctcagg cttctcaact actgtagaag tttcagctaa agctaaagcc ttccttgacg
     6301 cttaccgtgc taagtacaat gaagagcctt caacatttgc agccttggct tatgattcag
     6361 ttcaccttgt agcaaacgca gcaaaaggtg ctaaaaattc aggtgaaatc aaggataacc
     6421 ttgctaaaac aaaagatttt gaaggtgtaa ctggtcaaac aagcttcgat gcagaccaca
     6481 acacagtcaa aactgcttac atgatgacca tgaacaatgg taaagttgaa gcagcagaag
     6541 ttgtaaaacc ataatagaaa aatgttgaaa tagggaatga gcctttgact cactccctgt
     6601 ttcgatattt aatactcttc gaaaatctct tcaaact
//
LOCUS       DAVBCN010000091         6559 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVBCN010000091 DAVBCN010000000
VERSION     DAVBCN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6559
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.091"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            136..1098
                     /locus_tag="VQ497_001830"
     CDS             136..1098
                     /locus_tag="VQ497_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET2518105.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYDDTSISVSSNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVIIFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVEPLRYE"
     gene            1275..2282
                     /gene="gap"
                     /locus_tag="VQ497_001831"
     CDS             1275..2282
                     /gene="gap"
                     /locus_tag="VQ497_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET2518106.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2349..3185)
                     /locus_tag="VQ497_001832"
     CDS             complement(2349..3185)
                     /locus_tag="VQ497_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2518107.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQYLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETYVSRLKQFSNKTSLARCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            3311..5506
                     /locus_tag="VQ497_001833"
     CDS             3311..5506
                     /locus_tag="VQ497_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET2518108.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMASQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVAAVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIAYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5559..5711
                     /gene="rpmG"
                     /locus_tag="VQ497_001834"
     CDS             5559..5711
                     /gene="rpmG"
                     /locus_tag="VQ497_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2518109.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            5721..5897
                     /gene="secE"
                     /locus_tag="VQ497_001835"
     CDS             5721..5897
                     /gene="secE"
                     /locus_tag="VQ497_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET2518110.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            5953..6489
                     /gene="nusG"
                     /locus_tag="VQ497_001836"
     CDS             5953..6489
                     /gene="nusG"
                     /locus_tag="VQ497_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET2518111.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         2035 a         1144 c         1430 g         1950 t
ORIGIN      
        1 aagcacctca aaaaggtatt accaagttgc aatacctttt acgaggctct tttgtcttgt
       61 tcttgtttca attgactata tatcacggat acagttggga aagaagctat taaacatttg
      121 gagagtcctt ttgggatgaa catgtcaaat attactatag atgggcaaga aatggatctg
      181 agtcaaacga tgccttctta tgctcaagta gtgagtcttt atgatgatac aagtatctct
      241 gttagtagta atgagacgga caaagtgttg gctggctccc tctatactga tacaaatgaa
      301 cagggattaa cgattccaag cagtttacta aaaaattgga atgaacagac aggaaaaaat
      361 ttgacagcta atgatcttat tggcaaatca gtctcagcca gcattgtaga aagtgctgcc
      421 gaaactagta agattgctca atttcaaacg aagattgtac gtgtaatcaa tgatgaagat
      481 gacatggagg acagcaacag tttcatgctg tctcatcaaa tggaaacgat tttgaaagag
      541 gctggattta cgaaagctgt atcttatttt atcttggaac tcaaagatcc atcacagaca
      601 aaagtagtaa cagaagaatt acagaaaaat aagaagtata ctgtgctttc tcaacagaga
      661 gttcttgata ttgtgattat ctttattcgt gttattcagg gattattgat tgtgctttca
      721 tcacaagcta ttgtggtagc agcggttatg attggtatca ttatttacat caatatcatg
      781 cagcgttcca aggaaatagg tgtcatgaag gcagttggtt atcagaatcg tgatgtcaaa
      841 ggaattttta tttacgaggc tatctggatt gtaggcatcg ccttgctgct ggcatttttg
      901 gttgcacaag gggtgggaag tttggcgaat gcgattgtaa gtcactttta cccatccatc
      961 actaaagttt ttgaattaaa tcttttatct gttttaggaa ctctagtttt cgctctatta
     1021 cttggttatg tctcagccta cttcccggcg cgtaagatta gtaaaatgga tcctgtagaa
     1081 ccgttacgct atgaatagta agggattcct tgatttacag tcttaaaaag aaactaacat
     1141 agaaaaacaa tggacatact tgcaattcaa ctcaaatagt tgtataatag gtttgttgaa
     1201 aggtgatttg tagtgattca agttactctt ttcacaataa aaatttcatg attttcataa
     1261 ggaggaaatc actaatggta gttaaagttg gtattaacgg tttcggacgt atcggtcgtc
     1321 ttgctttccg tcgtatccaa aacgtagaag gtgttgaagt tacacgcatc aacgacctta
     1381 cagatccagt tatgcttgca cacttgttga aatacgacac aactcaaggt cgtttcgacg
     1441 gtactgttga agttaaagaa ggtggatttg aagttaacgg taaattcatc aaagtttctg
     1501 ctgaacgtga tccagaacaa atcgactggg ctactgacgg tgtagaaatc gttcttgaag
     1561 ctactggttt ctttgctaag aaagaagcag ctgaaaaaca ccttaaaggt ggagctaaaa
     1621 aagttgttat cactgctcct ggtggaaacg acgttaaaac agttgtattc aatactaacc
     1681 acgacgttct tgacggtact gaaacagtta tctcaggtgc ttcatgtact acaaactgct
     1741 tggctccaat ggctaaagct cttcaagaca actttggtgt tgttgaagga ttgatgacta
     1801 ctatccacgc ttacactggt gaccaaatga tccttgacgg accacaccgt ggtggtgacc
     1861 ttcgccgtgc tcgcgctggt gctgcaaaca tcgttcctaa ctcaactggt gctgcaaaag
     1921 ctatcggtct tgtaatccca gaattgaatg gtaaacttga tggatctgca caacgcgttc
     1981 caactccaac tggatcagtt actgaattgg tagcagttct tgaaaagaac gttactgttg
     2041 atgaagtgaa cgcagctatg aaagcagctt caaacgaatc atacggttac acagaagatc
     2101 caatcgtatc ttcagatatc gtaggtatgt cttacggttc attgtttgac gcaactcaaa
     2161 ctaaagttct tgacgttgac ggtaaacaat tggttaaagt tgtatcatgg tacgacaacg
     2221 aaatgtcata cactgcacaa cttgttcgta ctcttgaata cttcgcaaaa attgctaaat
     2281 aattcttgag ttgatagaaa gcaaggcttt gtggtcttgc tttttatatg gaaaaatgga
     2341 tgacacgatc atccattctt ttttaattct ttttcaaatg tatttgaaag ggtagtgaaa
     2401 gttagcttct ctaaagtaag tgggtgggta aaggaaagtc ggaaggcatg aagcataagc
     2461 cgacttgtct ttgatttact attatagaga gggtcgccca ggataggaag attatgatgc
     2521 gaaaggtgca cacgaatctg atgggttcgc cctgtcttta gcttgcaacg agccaaggaa
     2581 gtcttgtttg agaattgctt taatctgctt acatacgttt cagcatattg cccatttttt
     2641 gcatcaacta ttctttttct acgatcatgg cgatcacgtc caattttgtc tctgaaaaca
     2701 agttcttttc tgttgatatt tccatcaact agagcccaat attctctaga aatctctttt
     2761 ttctccaata agcgattgag aatgggcagg ataaaaggat ttttggcaaa gagaactaag
     2821 ccactggttt ccatgtccag acgatgaacg acatagcagg tttggccaac ataggtactg
     2881 acatggttaa gaagggcaat ttcgtttggt tgattaccat gcgttttcat cccctctggt
     2941 ttgtttacaa taatcaagta ttgatcttga taaacttcct gcactaagtc tgggttgccc
     3001 caagggatcg tcttttgggg ataatcttcc tcgtcaaaag tcaactggca aacatctcca
     3061 ggatttacga tttcgttcca gtggacttct tcttgattta tcaaaatatg tttcttgatt
     3121 ctcaaaaaat gacggatttt tctagggatg aggagttgtt cctcaagtaa ttgctttacc
     3181 gtcatttgag gtagagaggc gggtaatgta aatgtgaatt gcatacagat attgtaacaa
     3241 aaaaagccct atttggatag gaaatagcta aattcttgtc ttcctatgat gaagatgata
     3301 aaataaacgc atgaaattag ataaattatt tgagaaattt ctttctcttt ttaaaaaaga
     3361 aacaagtgaa ctagaggact ctgattctac tatcttacgt cgctctcgta gtgatcgaaa
     3421 aaaattagcc caagtaggtc cgattcgaaa attctggcgt cgttatcatc taacaaagat
     3481 tatccttata ctaggtttga gtgcaggctt gctagttgga atctatttgt ttgctgtagc
     3541 caagtcgacc aatgtcaatg atttgcaaaa tgccttgaaa actcggactc ttatttttga
     3601 ccgtgaagaa aaagaggctg gtgccttgtc tggtcaaaag ggaacctatg ttgagctgac
     3661 tgatatcagt aaaaacttgc agaatgctgt tattgcgaca gaagaccgtt ctttctataa
     3721 aaatgacggg attaactatg gccgtttctt cttggctatt gtcactgctg gacgttcagg
     3781 tggtggctct accattaccc aacagctggc taaaaacgcc tatttatcac aggatcaaac
     3841 tgttgagaga aaagcgaaag aatttttcct tgccttagaa ttaagcaaaa aatatagtaa
     3901 ggagcaaatt ctaaccatgt accttaacaa cgcttatttt ggaaatggtg tgtggggtgt
     3961 agaagatgcg agtaagaaat actttggagt ttctgcatca gaagtgagtc tggatcaagc
     4021 tgcgactctg gcagggatgc tcaaggggcc ggaactgtat aatcccttga attccgtaga
     4081 agattctact aatcggcgcg atactgtctt gcagaatatg gttgcagcag gatatattga
     4141 taaaaaccaa gaaaccgaag ctgctgaagt tgatatggct tcgcaattgc acgataagta
     4201 tgaaggaaaa atctcagatt accgttaccc ctcttatttt gatgcggtgg ttaatgaagc
     4261 tgtttccaag tataatctaa cagaggaaga gattgtcaat aatggctacc gcatttacac
     4321 agagctggac caaaactacc aagcaaatat gcagattgtt tatgaaaaca catcgctatt
     4381 tccgagggca gaggatggaa cgtttgctca atcaggaagt gtagctctcg aaccgaaaac
     4441 agggggagtt cgtggagttg tcggtcaagt tgctgacaat gataaaactg gattccggaa
     4501 tttcaactat gcaacccaat caaagcgtag tcctggttct acaattaagc ctttagttgt
     4561 ttatacgcca gcagttgaag caggctgggc tttgaataag cagttggata accataccat
     4621 gcagtatgac agctataagg ttgataacta tgcagggatc aaaacgagtc gagaagttcc
     4681 tatgtatcaa gccttggcag aatcgcttaa tctacctgct gttgccgctg ttaatgattt
     4741 gggtgtcgac aaggcttttg aggcaggcga aaaattcgga ctcaacatgg aaaaggtcga
     4801 ccgtgttctt ggtgtcgcct tgggaagcgg tgttgaaacc aaccctcttc aaatggctca
     4861 agcatacgct gcctttgcaa atgaaggttt aatgcctgaa gctcatttta ttagtagaat
     4921 tgaaaatgct agtggacaag ttattgcgag tcataaaaat tcacaaaaac gggtgattga
     4981 taagtctgta gctgacaaga tgaccagtat gatgttgggg actttcacca acggtaccgg
     5041 tattagttca tcgcctgcag actatattat ggcagggaaa actggaacaa ctgaagcagt
     5101 tttcaatccg gagtacacaa gtgaccagtg ggtaattgct tatactccgg atgtagtgat
     5161 tagccactgg cttggctttc ctaccactga tgaaaatcac tatctagctg gctctacttc
     5221 aaacggtgca gctcatgtct ttagaaacat tgccaatacc attttacctt atacgccagg
     5281 aagtaccttt acggttgaaa atgcttataa gcaaaatgga attgcaccag ccaatacaaa
     5341 aagacaagta caaaccaatg ataatagcca gacagatgat aatttgtctg atattcgagg
     5401 gcgtgcgcaa agtctagtag atgaggctag ccgggctatc tcagatgcga agattaagga
     5461 aaaggctcaa acaatatggg attcgatagt caatctattt cgctaagatg cttgtcaaag
     5521 cctagctttc ttgttataat ggataggatg gaggcgttat ggcactaaaa aaagcaagcc
     5581 tagcttgtgc ggtttgtggt tcgagaaact attcaatcaa gatcagcgga aaccccaagc
     5641 ctacacgact agaagtaaat aaattttgta agcattgtgg caagtacact acacacagag
     5701 aaacgagata ggagagagcg atgcgtttta ttggagatat ttttagactt cttaaagaca
     5761 caacgtggcc aactcgcaag gaaagctgga gagattttcg ttctatcatg gaatacacag
     5821 ctttctttgt agtaattatt tacatttttg accagttgat tgtttcaggt ttgattcgat
     5881 ttattaacat tttttagaag attagtggag ttaattacac tagaaatctt ctatattatg
     5941 aaaggaaata tcatggatag ttttgataaa gggtggtttg ttttacaaac ttattctggt
     6001 tatgaaaata aggtgaaaga aaatctatta caacgtgcac aaacctacaa tatgttggat
     6061 aatattctac gcgttgaaat tccaacacaa acagtgcaag ttgaaaaaaa tggaaagaga
     6121 aaagaagtag aagaaaatcg ctttccaggt tatgttcttg tagaaatggt catgacagat
     6181 gaagcttggt ttgttgttcg aaacacacca aatgttacag gatttgtcgg atcacacggg
     6241 aacagatcaa aaccaactcc attattggaa caagaaattc gtgacatttt ggtatctatg
     6301 ggacaaactg ttcaagaatt tgatttcgat gttgagattg gtcaaactgt acgtattatt
     6361 gatggtgctt ttgcagacta cactggtaag attacagaaa ttgataataa caaagtgaaa
     6421 atgattatct ctatgtttgg taatgacaca gttgcagaag taaacctaaa ccaaatcgca
     6481 gaattataac cctgagagag gctggagcct ctcttttttg tgcagtttag gagctaaagg
     6541 gaacagaatg gagaaaatg
//
LOCUS       DAVBCN010000092         6523 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVBCN010000092 DAVBCN010000000
VERSION     DAVBCN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.092"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            104..394
                     /locus_tag="VQ497_001837"
     CDS             104..394
                     /locus_tag="VQ497_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518112.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEYPELYAVAQTSGEIPEKNAKNCVRLSQLSLNKR"
     gene            418..1134
                     /locus_tag="VQ497_001838"
     CDS             418..1134
                     /locus_tag="VQ497_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2518113.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSIGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIISDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(1263..2078)
                     /locus_tag="VQ497_001839"
     CDS             complement(1263..2078)
                     /locus_tag="VQ497_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HET2518114.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(2075..2980)
                     /locus_tag="VQ497_001840"
     CDS             complement(2075..2980)
                     /locus_tag="VQ497_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HET2518115.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGMLFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
     gene            3378..5507
                     /locus_tag="VQ497_001841"
     CDS             3378..5507
                     /locus_tag="VQ497_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HET2518116.1"
                     /translation="MDKKYEKISQDLGVMLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRAQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYTAVKELFKRL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVSVGD
                     LVTVWVKKIDTEREKVNLSLLAPNETD"
     gene            5494..5943
                     /locus_tag="VQ497_001842"
     CDS             5494..5943
                     /locus_tag="VQ497_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HET2518117.1"
                     /translation="MKLTDYVKQVSLEDFGRAFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQKLGMEVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPLKLYRCQSCQQRYQRKRRIDTKRYRCGLCRGKLLLINQPED"
     gene            6002..6274
                     /locus_tag="VQ497_001843"
     CDS             6002..6274
                     /locus_tag="VQ497_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET2518118.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
BASE COUNT         2044 a         1191 c         1417 g         1871 t
ORIGIN      
        1 tgctaaaatc ttatcagtga gaagatttct taaagtttat tctttgtagt ttcatctgta
       61 ttagtacata atttaactta gagaaaatag gaggactaat cgaatggaat taaaagattt
      121 tacagaaaaa gaacaggaaa tgattaagaa aaggcttaca atgtctaata ttagtgacaa
      181 agaaactact gagaagattc tggcgctagt cccacaagac ttgattaagc gaatcccgtt
      241 ttttgtcaga aaacatgcta caacacgtac gattaaacgt atttcaattg aataccctga
      301 actctacgct gtagctcaaa caagtggtga gattccagaa aaaaacgcga agaattgcgt
      361 cagattatca caactatctt tgaacaaaag atgaataagc atagtattaa gtagagaatg
      421 aaaaaatatt ttattggcgg tttgggaagc aatgcctatc atagcaagga ttttcttcaa
      481 gaactagatt cgcaggtcta ttttctaaat ccatatgaaa agcatcttcg agatgaaaca
      541 gaattgaaat catggtttaa aaatgagatt gtagaggaag aatctatctg tctgataggt
      601 cattctattg gaggcgattt agctcgttat ttcgcatcgg aatttgaaga agtaaagaaa
      661 ctgattcttt tggatggtgg ctatctagat ttagataaga ttttaccttt ggatacagag
      721 ttagaggaaa ctaaaaatta tatcaaatct caaattattt cggacttaga tgttcttact
      781 tctaaagaaa aatctgaagc aaagcattgg tcagaaaata tggagaaagc tgtaagacag
      841 tcctatcact ggaatgttga gtataataga tatgagttgg ctataaatta tgaaaatata
      901 gaagcgatac tccgcctacg gagaaaaata caagctttta agagagaagt gggagatacc
      961 ttgtttatta gtcctcgcta tcctaatgaa gctacatgga gagaggaagc cctaaaagaa
     1021 ttgccagact attttgatac tattttttta gaaaactttg gccatgagct ttatactcaa
     1081 gcacctaaag aaatcgctag tctaatgaat gagtggctcg cttattttct atgagctact
     1141 tgtattgctg gaaatggtat aagatgaaaa tctacaagtc actaattttc atataacaaa
     1201 aaacgaacag gctctttttc tgttattcag aagagaactt gttcgttttt atcttgattt
     1261 tcttaaaaag ctctatatac atagggattt tctggtttat cgactttggt aaagctgtcg
     1321 atttccaagg ttggaaggtt ttgtttgagt tctttatggt agtgattgac cagttttcct
     1381 ttggctgtta tccaagtgtt gttttcatac tgccgggtat tgcccttggt cagcaatcca
     1441 tagacaccag aatctgcgat acagtggata atgccgaatc ggaacagaaa ttgactattg
     1501 gcatgactgg ggtcgttata gacaaagcct gtaaactgga ttgtcttgcc ctcaaactca
     1561 tctggatagt cgtagatagc ctccatgact tccatatagt tttcattagt gatctgaata
     1621 ctatcttggg ataagtattt atccgccgcc gttcgcattt ccttttcata ggctgatttt
     1681 gaaaaataag aactggtatc tggtttcaaa tattggcttg tcgtcccttc gcttgtctga
     1741 atggctagat ctgttccttc cgataggggg aaatgataac ctttagcaga aacggtctga
     1801 gaatccaagc taacagttgg gaaagttaag ccgatgacaa gtggaatagc cagaagagaa
     1861 atactcgtca tcttggccaa tcggctgttc agatgactgt gggttttgac ttgcttcatc
     1921 cagatataca attgaacaat tgccaagata aaggaaagca ccatggagat ataggccaga
     1981 taggaatagt gcatgttgat gtactggttt agtttgcccg acagatggag gtaaatagtc
     2041 agttcaaaat agccagctaa aactaaaaat cgaatcatag gatgactcca atcaagagag
     2101 aatagactaa gacgacaaga gttacaatcg ttatgaagtg actgataaat cgtgctttca
     2161 agtaattttt catcatgaga atatttttga tatccagcat tggaccaatg acgagaaagg
     2221 ccagaactgg tgccaaaccg aaactcgaga gaagagaagc acctataaag gcatccgcct
     2281 cactacagag cgaaagaaga aaggccaaaa tcatcaagag caggatggca aaaagaggag
     2341 tcgcactgat agaggtcaga atccgagtcg gaacgtagac ctgtattata gaagcaaaga
     2401 ggcagccaaa taccaaataa cgccccgtat caaaaaattc atcaatggcc tgcacaaaga
     2461 cttgaaaaac tttttttgca gaactcaagt aagaaaaatc atgctcatga caagccagac
     2521 gattttcttt ctgaatcggt tcttgccaga aaaatcctag aaaaattcct agtattacag
     2581 ccacaagaat ggaacccaga gctcgtaata gggcgacatg gaaggagttg ccaaaggcag
     2641 aataggtcgc aaaaagaaca atgggattga taacaggtgc tgtcacaaga aaaggaacgg
     2701 ctgtgtaact gggaaccttt ttttccagaa aacgattgat gatggggacg attccacatt
     2761 cacaagaagg gaaaagcata ccgacaaagg tcccaaaaaa gattctcccc caacgatttc
     2821 gagggagaaa atgataaacc ttgtcaggtg tgatataaac ttcaatcagc cctgagacaa
     2881 tgcttcctat tagaacgaag ggaagggctt caatgataat ggagagaaaa atggctccag
     2941 tttgaagtac actagatggg agttgttgaa aagcagtcat ctagtttttc ttttctgctt
     3001 tggctttttt ctctttgtca tcagggaaag taacttgttt caaatcaggg agtttagcaa
     3061 actcttcaaa catcttgtcc aagtcgtctc gttttgtaaa ttttacagcc ataggtgtac
     3121 ctcgattcta aagtctacct ctattatact attattttgc ggaaaaggga gttttttaat
     3181 ttgttttcga tagttgatac acttcgaaaa tctcttcaaa ccacgtcagc ttcgccttac
     3241 cgtatatatg tgacttatcc acaacctcaa aacagtattt tgagcagcct gcggctagct
     3301 tcctagtttg ctttttgatt ttcattgagt atgaaaagga ttggagaaag ccttccagct
     3361 tgtggtataa tagatttatg gataaaaaat atgaaaaaat ctctcaggat ttgggagtga
     3421 tgttaaagca aattgatacc gttctaagtt tgacagctga aggggcgact attcccttta
     3481 tcgcgcgtta tcgcaaggac atgactggta gtctggatga ggtggcgatt aaggctatta
     3541 ttgatttgga taaaagtctg acaaatctca atgaccgtaa ggaagctgtc ttagctaaga
     3601 ttcaagaaca aggtaagttg accaaggaat tggaagaagc tatcttagtt gccgaaaaat
     3661 tagcagacgt tgaagaactc tatcttcctt ataaggaaaa gcgtcgtacc aaggcaacca
     3721 ttgcccgtga agctggactc tttcctcttg ctcgtttgat tttgcagaat atagttgact
     3781 tagagaaaga agctgaaaag ttcgtctgtg aaggatttgc gactggcaag gaagccttga
     3841 ccggtgcagt tgatattttg gtcgaagcct tatcggaaga tgtgaccttg cgttctatga
     3901 cttatcagga agtgctgaga cactctaaac ttacttctca agccaaggat gaaagtcttg
     3961 atgaaaagca ggtttttcag atttattatg atttttcaga gacagttgga actatgcaag
     4021 gctatcgtac cttggctctc aatcgtgggg agaaacttgg tgtcttgaag atcggttttg
     4081 aacatgcgac ggaccgtatt cttgccttct ttgctactcg tttcaaggtg aaaaatgctt
     4141 atattgatga agttgttcag caatccgtta agaaaaaggt cttgcctgct atcgagcgtc
     4201 gtattcggac agaattaact gagaaagctg aagaaggagc tatccaactt ttttctgaca
     4261 atctgcgcaa tctcctcttg gttgctccac tgaaagggcg cgtggttctt ggatttgacc
     4321 cagcctttcg tacaggtgcc aagttagctg tcgtggatgc aacaggaaaa atgctgacaa
     4381 ctcaggttat ttatcctgtt aaaccagcat cagctcgtca aatcgaagaa gccaagaaag
     4441 atttagcaga tttaattggt caatacggtg tagagattat tgccattgga aatggaacgg
     4501 ccagtcgtga aagtgaagct tttgtagcgg aagttctgaa agatttccct gaagtcagct
     4561 atgttatcgt taatgaaagt ggtgcttctg tctattctgc cagcgaactt gctcgtcagg
     4621 agtttccaga cttgaccgtt gaaaaacgct ctgccatttc tatcgcccgt cgtttgcaag
     4681 atcctcttgc ggaattggtc aaaatcgatc ctaagtcaat tggtgtcggt caataccaac
     4741 acgatgtcag tcagaagaaa ctatctgaga gtctggactt tgttgtcgat acagtggtta
     4801 accaagttgg tgtcaatgtc aatacagcta gcccagctct tctttcacac gtagctggac
     4861 tcaacaaaac tatctctgaa aatattgtca aataccgcga ggaagaagga aaaatcactt
     4921 cacgcgccca aatcaagaaa gttcctcgtc tgggagccaa ggcctttgag caggctgctg
     4981 gtttccttcg tatccctgaa agtagcaata tccttgataa tacaggagtt cacccagaga
     5041 attacactgc agttaaggaa ctcttcaaac gtttagatat taaagaccta aacgaagaag
     5101 cccaaagcaa actcaagtcc ctttcagtca aggaaatggc tcaagagcta gacctcggtc
     5161 cagaaaccct taaagatatc attgcagatc ttctcaaacc aggtcgagat ttccgtgatt
     5221 cctttgacgc acctgtgctt cgccaagatg tcctagatat caaagaccta gtggtaggtc
     5281 agaagctaga aggtgttgtg cgtaacgttg ttgacttcgg tgccttcgtt gatatcggga
     5341 ttcacgagga cggcttgatt catatttctc atatgagtcg caagtttatc aaacacccta
     5401 gtcaagtggt gtcagttgga gatttggtga ccgtttgggt caagaaaatt gatactgaac
     5461 gtgaaaaagt taatctgtcg ctcctcgctc caaatgaaac tgactgatta tgtcaagcag
     5521 gtttcactag aagacttcgg gagagctttt atccatcatg tccagtggaa taggcgtcta
     5581 cgttcgacag gtgggcgatt tttccccaaa gatgggcatc tggattttaa tcccaaggtt
     5641 tatcagaaac tgggtatgga ggtttttagg aaaattgtcc gacatgaact ctgtcattat
     5701 cacctttatt tccaagggaa gggctatcaa cacaaggatc gggattttaa ggaactgttg
     5761 aaagcagtgg atggattaag gtttgtacca tccttgccca atagtaactc taaaccactc
     5821 aagctttatc gttgtcaatc ctgccagcaa aggtatcagc gcaagcgtag gattgatacc
     5881 aaacgctatc gctgtggact ttgtcgaggt aaattgcttc tgataaatca gcctgaggac
     5941 tgatgaaagc cgagcctccc cgtgatatac tatgtctaac ctagcagaaa gaggaaatat
     6001 catgaacagt aaattttata aaatgagacg caatcgcatg atttcaggag ttctggcagg
     6061 tctgtcagac aagtggaatt ttgatgttac cctagttcgt ttcttgtttg ccatttttac
     6121 agttgcaaac tttggacttg gcgtgattat ctatatcatc ctagcttcta tcatgcctac
     6181 caaggaagaa atcgaagcag aaatgtacgg aacaggccca cgtaaacgca aagaagccca
     6241 agccattgac gacaatgaag gctggttttg gtgaggttct acagaagatt tggaataagc
     6301 aaaaagcttt aaatcaatag tctaaactgc ttgatttaaa gcttttttac ttttatactc
     6361 aataaaaatc aaagtgcaaa ctaggaagcc agccacaggc tgtaatttag tcggcaatgt
     6421 gaagttgaca tagtttgcac tttgattttt gaatagtatt aataatattt cgccagcttc
     6481 atccacaaac tatagtggat tggaactaga atagtacacc tct
//
LOCUS       DAVBCN010000093         6489 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVBCN010000093 DAVBCN010000000
VERSION     DAVBCN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.093"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..211
                     /locus_tag="VQ497_001844"
     CDS             <1..211
                     /locus_tag="VQ497_001844"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518119.1"
                     /translation="PNPSNRRDDRVELSHQVIDTFFRLVNEPLVMFDDFITRDKTSMR
                     YQTHYQLKQKAVVRSFRKSSSDWRK"
     gene            298..2064
                     /gene="patB"
                     /locus_tag="VQ497_001845"
     CDS             298..2064
                     /gene="patB"
                     /locus_tag="VQ497_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HET2518120.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTHVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            2203..2892
                     /locus_tag="VQ497_001846"
     CDS             2203..2892
                     /locus_tag="VQ497_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET2518121.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLDEL"
     gene            2896..3075
                     /locus_tag="VQ497_001847"
     CDS             2896..3075
                     /locus_tag="VQ497_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518122.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            3062..4411
                     /locus_tag="VQ497_001848"
     CDS             3062..4411
                     /locus_tag="VQ497_001848"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET2518123.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            4535..5995
                     /locus_tag="VQ497_001849"
     CDS             4535..5995
                     /locus_tag="VQ497_001849"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET2518124.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            6009..6443
                     /locus_tag="VQ497_001850"
     CDS             6009..6443
                     /locus_tag="VQ497_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET2518125.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNTYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT         2031 a         1250 c         1396 g         1812 t
ORIGIN      
        1 tcccaatccc agtaaccgtc gtgatgaccg agttgaactc agccatcaag tcattgacac
       61 atttttccgc cttgtcaatg agcctcttgt aatgtttgat gatttcatta cacgagataa
      121 aacgtctatg cgttatcaaa ctcattacca attaaaacaa aaagctgtgg ttagatcctt
      181 tcggaaatcg tcaagcgatt ggaggaaatg aactaatcca cagcggctta ttccaagtat
      241 accacttggg ctttggcagt agctaactgc gctaaatata atataaggag gagtaaaatg
      301 aagacagttc aatttttttg gcattatttt aaggtctaca agttctcatt tgtagttgtc
      361 atcctgatga ttgttctggc gacttttgcc caagccctct ttccagtctt ttctggacaa
      421 gcggtgacgc agctagccaa tttagttcaa gcttatcaaa atggcaatcc agaacttgta
      481 tggcaaagcc tatcaggaat catggtcaat cttggcctgc tggttttggt tctatttatc
      541 tctagtgtaa tatacatgtg tctcatgacg cacgtgattg cagaatcgac caacgagatg
      601 cgcaaaggcc tctttggtaa gcttgctcag ttgacggttt ctttctttga ccgtcgacaa
      661 gatggcgata tcttgtctca ttttaccagt gatttggata atatcctcca agcctttaac
      721 gaaagcttga ttcaggtcat gagcaatatt gttttataca ttggtctgat tcttgtcatg
      781 ttttcgagaa atgtgacgct ggctctcatc accattgcca gcaccccatt ggctttcctt
      841 atgctgattt tcatcgtgaa aatggcacgc aaatacacca acctccagca gaaagaggta
      901 gggaagctca acgcctatat ggatgagagc atctcaggcc aaaaagccgt gattgtgcaa
      961 ggaattcaag aggatatgat ggcaggattt cttgaacaaa atgagcgcgt gcgcaaggca
     1021 acctttaaag gaagaatgtt ctcaggaatt cttttccctg tcatgaatgg gatgagcctg
     1081 attaatacag ccatcgtcat ctttgctggt tcggctgtac ttttgaatga taagtctatt
     1141 gaaacaagta cagccctagg tttgattgtt atgtttgcac aattttcaca gcagtactac
     1201 cagcctatta tccaagttgc agcgagttgg ggaagccttc agttggcctt tactggagct
     1261 gaacgaattc aggaaatgtt tgatgcagag gaggaaatcc gacctgaaaa ggctccaacc
     1321 ttcactaagt tgcaagaaag tgttgaaatc agtcatatcg atttttcata cttgcctgat
     1381 aaacctattt tgaaagatgt cagcatttct gcccctaaag gccagatgac agcagttgtt
     1441 gggccgacag gttcaggaaa aacgactatt atgaacctca tcaatcgctt ttatgatgtt
     1501 gatgctggtg gtatttattt tgatggtaaa gacattcgtg gctatgactt agatagtctt
     1561 agaagcaagg tgggaattgt attgcaagat tcggtcttgt ttagcggaac gattcgagac
     1621 aatatccgat ttggtgtgcc agatgctagt caggaaatgg ttgaggtagc agcaaaagca
     1681 acccacattc acgactatat cgaaagtttg cctgataagt acgatactct tattgatgat
     1741 gaccagagca tcttttcaac agggcagaag caattgattt caatcgctcg aaccctgatg
     1801 acagatccag aagttctcat tctcgatgaa gcaacttcaa acgtagatac ggtgacagaa
     1861 agcaagattc agcatgccat ggaggtggtt gtagcaggta gaactagttt cgtcattgcc
     1921 caccgcttga aaaccattct caatgcagat cagattattg tccttaaaga tggagaagtc
     1981 attgaacgtg gtaaccacca tgaacttttg aagctaggtg gcttttattc agaactctat
     2041 cacaatcaat ttgttttcga ataagaaaga agttgtccta tgtgggcagc tttttcttgt
     2101 ccataaaaaa tgtttatcac agccttaaaa aaaacatatt agacgaaagt cattttgagt
     2161 gatatgatag gactatcgtt agcattcgaa aggagaggca tcatggctag aacggttgta
     2221 ggagttgctg caaatctatg tcccgtagac gcagaaggca aaatcattca ttcatctgta
     2281 tcttgtagat tcgcagagat cattcgtcaa gtcggtggtc tccctttagt cattcctgtt
     2341 ggtgatgagt cagttgtacg tgattatgtg gaaatgattg acaaactcat tttgacagga
     2401 ggccaaaatg ttcatcctca gttttatgga gagaaaaaga ccgtcgagag cgatgattac
     2461 aatctggtcc gtgacgaatt tgaattggca ctcttgaagg aagcgcttcg tcagaataaa
     2521 ccaattatgg caatctgtcg cggtgtccaa cttgtcaatg ttgcctttgg tggaaccctc
     2581 aatcaagaaa tcgaaggtca ctggcaagga ctacctttcg ggacatctca ctctattgag
     2641 acagtagaag gaagcgtggt ggctaagcta tttgggaaag aaagtcaggt caactcagtc
     2701 catcgtcaaa gcattaaaga tttggcacct aatttccgtg taactgctat tgattcaaga
     2761 gaccaaacta ttgaagcgat tgagtctatc gacgagcacc gcattatcgg tttacagtgg
     2821 catccagagt ttctggttaa tgaagaagat ggcaatttag aattatttga gtatttattg
     2881 gatgaactgt aacaattgga acatctggtc gttctttctt ttattttttc aaaattttta
     2941 gaatgtggta ttttacgcaa acgtttgaat tctgataaaa attggagaaa ttcgacaaaa
     3001 aaacttgaaa aaaacgaagg taagcgttat gatagaaaag aagaaatatt ggaggaagaa
     3061 catgtcacat attaaatttg attattcaaa agttttagac aaatttgttg caccacatga
     3121 agtggaatac atgcaatcac aagtaacagc agcagatgaa ttgatccgta aaggaactgg
     3181 tgctggtagc gacttcttgg gatggttgga ccttcctgaa aaatacgacc gcgaagaatt
     3241 tgaccgcatc ttgaaagctg ctgagcaaat caagtcagac agcgatgttt tggttgtgat
     3301 cggtattggt ggatcttacc ttggtgcaaa agcagcaatc gacttcttga atcaccattt
     3361 tgctaacttg caaacaaaag aagaacgcaa agctccacaa atcctttacg caggaaactc
     3421 aatctcatct acttaccttg ctgacttggt agagtatgtt gcagacaaag acttctcagt
     3481 aaacgtaatt tctaaatcag gtacaacaac tgaaccagcg attgctttcc gtgtctttaa
     3541 agaactcttg gttaagaaat acggtcaaga agaagctaac aaacgtatct atgcaacaac
     3601 tgaccgccaa aagggtgctg ttaaggttga agcagacgct aacggttggg gaacatttgt
     3661 tgttccagat gatatcggtg gacgcttctc agtattgaca gccgttggtt tgcttccaat
     3721 cgcagcatca ggagctgaca taaaagctct tatggaaggt gcgaatgcag ctcgcaaaga
     3781 ctacacttca gacaaaatct ctgaaaacga agcttaccaa tacgcagctg ttcgtaacat
     3841 cctttatcgt aaaggctatg caactgagat cttggtaaac tatgagccat cacttcaata
     3901 cttctcagaa tggtggaaac aattggctgg tgaatcagaa ggaaaagacc aaaaaggtat
     3961 ctacccaact tcagccaact tctcaactga cttgcactca cttggtcaat ttatccaaga
     4021 aggaactcgt atcatgtttg aaacagttgt ccgtgttgac aaacctcgta aaaacgtgct
     4081 tattcctact ttggaagaag accttgacgg acttggttac cttcaaggaa aagacgttga
     4141 ctttgtaaac aaaaaagcaa ctgacggtgt tcttcttgcc cacacagatg gtgatgtacc
     4201 aaacatgtat gtgactcttc cagagcaaga cgctttcact cttggttaca ctatctactt
     4261 cttcgaattg gcaattgccc tttcaggtta cttgaatgct atcaacccat ttgaccaacc
     4321 aggtgttgaa gcttataaac gtaacatgtt tgcccttctt ggaaaaccag gatttgaaga
     4381 attgagcaaa gaacttaacg cacgtctata atagaagaaa agagtggttt gcccactctt
     4441 tttactctct ttatccatag aaattggact cagccaagac ttgtgatata atatagaaag
     4501 caaaaaggca gacgcctaga taataggaga aactatgtca aaagatatcc gcgtacgtta
     4561 cgcaccaagt ccaacaggac tactacacat cggaaatgct cgtacagcat tgtttaatta
     4621 cttgtatgcg cgccatcatg gtggaacatt tctcatccgt atcgaagata ctgaccgtaa
     4681 acgccatgtc gaggatggtg aacgttcaca acttgaaaac cttcgctggt taggcatgga
     4741 ttgggatgaa agtccagaat cacatgagaa ttatcgccag tctgagcgtt tggacttgta
     4801 tcaaaaatac atcgaccaac tattagctga aggaaaagcc tacaaatctt acgttacaga
     4861 agaagagttg gcagctgaac gtgaacgtca agaagtagct ggcgaaacac cacgctacat
     4921 caatgaatac cttggtatga gtgaagaaga aaaagcagct tacatcgcag aacgtgaagc
     4981 agcagggatc atcccaactg ttcgtttggc tgtcaatgag tcaggtatct acaagtggca
     5041 tgatatggtc aaaggcgata tcgaatttga aggtggcaat atcggtggtg actgggttat
     5101 ccaaaagaaa gacggttacc caacttacaa ctttgccgtt gttatcgatg accacgatat
     5161 gcaaatctct catgttatcc gtggagatga ccatattgct aatacaccaa aacagcttat
     5221 ggtctatgaa gctcttggtt gggaagctcc agagttcggt cacatgacct tgattatcaa
     5281 ctctgagact ggtaagaagt tatctaaacg tgatactaat acacttcaat ttatcgaaga
     5341 ttaccgaaag aaaggttacc ttccagaagc agtctttaat tttattgctc ttcttggttg
     5401 gaacccaggt ggtgaagatg agattttctc tcgtgaagaa ctcattaaac tttttgatga
     5461 aaaccgcctc agcaagtcac cagcagcctt tgatcagaaa aaactagact ggatgagcaa
     5521 tgattatatc aagaatgcag accttgaaac catctttgaa atggcaaaac cattcttaga
     5581 ggaagcaggc cgtttgactg acaaggctga aaaattagtt gagctctata aaccacaaat
     5641 gaaatcagta gatgagatta tcccattgac agatcttttc ttctcagatt tcccagaatt
     5701 gacagaagca gagcgcgaag tcatgacggg tgaaacagtt ccaacagttc ttgaagcatt
     5761 caaagcaaaa cttgaagcga tgacagatga taaatttgtg acagaaaata tcttcccaca
     5821 aattaaagca gttcaaaaag aaacaggtat taaagggaaa aatcttttca tgcctattcg
     5881 tatcgcagtt tcaggcgaaa tgcatgggcc agaattacca gatacaattt tcttgcttgg
     5941 acgtgaaaaa tcaattcagc atatcgaaaa catgctaaaa gaaatctcta aataaagagg
     6001 atacaataat ggacatctgg gaaaagatgt acgaagaagc acagaaacta tacaatccac
     6061 atgaagtatc agactttgtt tatgccaatc atgttgtagc tgcagtagaa gcagaagatg
     6121 gacaaatatt tacaggattc tgtatggagg gaacctgtgg tgttttccat ctctgcgcag
     6181 aacgggcggc cctcttcaat acgtaccaat tttcaggaca aactaaggtt aagaaagtat
     6241 tagcctttcg agacaaacca ccttatggtg gaagttcagc catgccttgc ggtgcttgta
     6301 gagaattcct tttagagctt aatgctgaaa ataaagatgc agaattcatg atggactata
     6361 atataagaaa aacagttaaa gtcgcagaac taatccctta ttggtgggga gaagaacgtg
     6421 cttctaagtt taatgagcga tagaagagtc agtataattc tggctttttt tactataagt
     6481 tgaaaaaat
//
LOCUS       DAVBCN010000094         6434 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVBCN010000094 DAVBCN010000000
VERSION     DAVBCN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6434
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.094"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            209..409
                     /locus_tag="VQ497_001851"
     CDS             209..409
                     /locus_tag="VQ497_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518126.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            434..850
                     /locus_tag="VQ497_001852"
     CDS             434..850
                     /locus_tag="VQ497_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518127.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1081..1788
                     /gene="ccdA2"
                     /locus_tag="VQ497_001853"
     CDS             1081..1788
                     /gene="ccdA2"
                     /locus_tag="VQ497_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET2518128.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1799..2356
                     /gene="sdbB"
                     /locus_tag="VQ497_001854"
     CDS             1799..2356
                     /gene="sdbB"
                     /locus_tag="VQ497_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HET2518129.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2477..3868
                     /locus_tag="VQ497_001855"
     CDS             2477..3868
                     /locus_tag="VQ497_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET2518130.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPSWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4011..4928
                     /gene="adcAII"
                     /locus_tag="VQ497_001856"
     CDS             4011..4928
                     /gene="adcAII"
                     /locus_tag="VQ497_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HET2518131.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            4936..>6434
                     /locus_tag="VQ497_001857"
     CDS             4936..>6434
                     /locus_tag="VQ497_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2518132.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGNHFHYIPKSDL
                     SASELAAAQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLVFDPAQITYRTVNGVAVPHGDHYHFIPYSQLSPLEEKLA
                     RMIAVKGQNGAVLPGMHYLKPAPKPQVQPSTEKKQTDFAVEQVVRKVGEGYVVEIAGV
                     SHYVFAKDLAKDKIDAIENLLSKKTQETHALVAKKENVAPRDQEFYDKAYNLLTQAHK
                     VLSENKGRTSDFQALDKLAERLNNESSNKVKLVDDLLAFLAPITHPERLG"
BASE COUNT         1975 a         1170 c         1351 g         1938 t
ORIGIN      
        1 tttctcttac caacattaac cacaccatcg gttattatag taaaatggaa taagaacagg
       61 acaaatcgat caggacagta aaatcgaatt ctaacaatgt tttagaagtc tatatgtact
      121 attctagttt caatatacca taactgctcc tagtcaacct gcaagtcctg tcccagctcc
      181 aagctcagct ccgactcaag atcctagagt ggtagcaagc gcttcggcta gctcaacgag
      241 cacacaagct caagagcaag ttgacaagtc tgaacttcgt gccttgagtc aagagttaga
      301 ccaacgcttg aaagctttgg caacagtatc tgatccaaaa atcgatgcaa ccaaggctgt
      361 cctcctagat gctcaaaaag ctccggaaga tagtgccttg acagagtaag gtctgcatac
      421 tgcagtagag tctgtgaagg ctgccttgga ctctctcaaa gatgtgaaag caaatgctag
      481 cgacagcaag cctgcacagg acaagaagga tgcaaaacaa ggaacggaag atagtaagga
      541 ttcagataag atgactgaaa caaactcagt tccggcagga gtgattgtgg tcagtctact
      601 tgccctccta ggcgtgattg ccttctggct gattcgccgt aagaaagagt cagaaatcca
      661 gcaattaagc acggaattga tcaaggttct aggacagcta gatgcagaaa aagcggataa
      721 aaaagtcctt gccaaagccc aaaaccttct ccaagaaacc cttgatttcg tgaaagaaga
      781 aaatggctca gcagagacag aaactaaact agtagaggag cttaaagcaa tccttgacaa
      841 actcaagtaa aacatgattt atgaaatgtc agacctaaaa gacattttcg ttcacaaaag
      901 cagtgacgag taatccttgt cgttgctttt tggctatttt gggggataag atccgccttg
      961 actagcaaaa gaaaagattg gtccaatagt gcccgatacg attttgcgta gcagaaagct
     1021 tacaaataaa attgaatata gtaaaatagg attagaatta ttaagaaagt tggttcttta
     1081 ttggaaacga tagtattttt aatctctgtt tttctagcag gtgttttatc ctttttttct
     1141 ccttgtattt ttcctcttct gccagtctat gctgggattt tattggatga tcaagaaagt
     1201 gcaaaaagct tttctttgtt tgggagaaag gttctctggt caggcttgat tcgaacactt
     1261 tgctttatcg ctggtatctc tctcattttc tttattctag gttttggtgc tggttacttt
     1321 ggtcatattc tctatgcaaa ttggtttcga tatgtcatgg gagctattat tatcattttg
     1381 ggtcttcacc agatggaaat ttttcatttg aagaaattag aagttcaaaa aagttttacc
     1441 tttaaaaaat cagattctaa tcgttattgg tcagcttttt tacttggtat tacctttagc
     1501 tttggttgga caccttgtat tggtccagtt ttaagttctg ttttagcact tgcggcttct
     1561 ggaggcaatg gtgcttggca aggagcgatt tatactctca tttacactct gggcatggcc
     1621 cttcctttct tggtcttggc actagcttca ggtctagtca tgccatattt tagtaaaatc
     1681 aagcgtcata tgatgctact aaagaaaatt ggtggtttcc tcattgtttt aatgggaatt
     1741 ttgttactat taggacaagt aaatgttcta gctggaattt ttgaataaag gaggacaaat
     1801 gaaaaaagta atgtttgctg gcttaagtct cttgtcatta gttgtattga tggcctgtgg
     1861 tgaggaagaa actaaaaaga ctcaagcagc acaacagcca aaacaacaaa cgactgtaca
     1921 acaaattgct gttggaaaag atgctccaga cttcacattg caatccatgg atggcaaaga
     1981 agttaagtta tctgatttta agggtaaaaa ggtttacttg aagttttggg cttcatggtg
     2041 tggtccatgc aagaaaagta tgccagagtt gatggaacta gcggcgaaac cagatcgtga
     2101 tttcgaaatt cttactgtca ttgcaccagg aattcaaggt gaaaaaactg ttgagcaatt
     2161 cccacaatgg ttccaggaac aaggatataa ggatatccca gttctttatg ataccaaagc
     2221 aaccaccttc caagcttatc aaattcgaag cattcctaca gaatatttaa ttgatagcca
     2281 aggaaagatt ggaaagattc aatttggtgc tatcagtaat gcggatgcag aagcagcatt
     2341 taaagaaatg aactagaatc gataaaatct gattacaaga tgtagtcaga tttttttaga
     2401 aaaacatttt ataaatattc acatatctcc tatatttatg gtaaaataga attatcagtt
     2461 tattttggag tcaaagatga atatatttag aacaaagaat gttagtttag ataaaacaga
     2521 gatgcatagg catttgaagt tatgggattt gattttgctg ggtatcggag ccatggtagg
     2581 gacaggcgtc tttacaatca caggtactgc agctgcaaca cttgctggcc cagccctagt
     2641 gatttcaatc gttatttctg ccttgtgtgt gggattatca gccctctttt ttgcagaatt
     2701 tgcctcgcga gtacccgcta caggaggtgc ctatagttac ctctatgcta tcttaggaga
     2761 attcccttcc tggttggctg gttggttaac catgatggag ttcatgacag ccatatcagg
     2821 cgtagcttcg ggttgggcag cttattttaa aggcttgctc tctcaatacg ggatagcctt
     2881 tcctcaggct ttaaatggta cctttaatcc tcaagcaggg acatttgttg atttattgcc
     2941 tattcttgtc ttggtcttgg tgacttcgct tgttttacta aatgccaagg cagccttacg
     3001 ctttaattcg attctagtta ttttgaaatt ttccgcttta gctctctttg ttttagtagg
     3061 aatttggaat attaagtttg ataattggag caattttgct ccttacggtt ttggacaaat
     3121 ctatggagct agcactggta ttatggctgg tgcgtccttg atgttcttcg gttttttggg
     3181 atttgaatcc atctctatgg ctgtagatga agtcaagact cctcaaaaaa atattcctag
     3241 agggattgtc ttatcgcttt ctatcgtaac cattctctat gccttggtga cccttgtttt
     3301 gactggtgtg gttcactata gtcatttaaa tgtcgatgat gccgttgcct ttgcccttcg
     3361 tagtgttggg attagttggg cagccaacta tgtgtcatta gtggctatct tgaccttgat
     3421 tacagtttgt atttcgatga cctatgccct atcgcgtatg atttacagtt tagcacgtga
     3481 cggcttagtg cctgctgcct ttaaggaact gacgaagact agcaagatac caaagaatgc
     3541 tactatcttg acaggtctag cttcagcagt agcagcagga atgttcccac tagccagtat
     3601 tgcagccttt ttaaatattt gtaccttagc ctacttgatc atgctggctt atggtctgat
     3661 tcgcttacgg aaagaaaaag gaatgcccaa agctggggaa tttaaaacac cattggtacc
     3721 tctgttacca attttatcga tcatcatttg tttatccttc atgttacagt ataatatgaa
     3781 cacctggatt gctttcctag ttgcgttgtt agtaggaagt attatttact tcacttatgg
     3841 ctataagcat tctaccatag aagaataaag tgaaagtcta ttgagttgtt gacactcagt
     3901 agatttttat atgtgaaaga aatttcaaat tttttctaaa aatatcttga cagattaaat
     3961 tttcaggagt agaatattta ctagttaatt aaatggttaa ggagttgttc atgaagaaac
     4021 aaaatttatt tttagtcctg ttaagtgtct ttcttttgtg tttgggggct tgtggtcaaa
     4081 aggaaagtca gacaggaaag gggatgaaaa ttgtgaccag tttttatcct atctacgcta
     4141 tggttaagga agtatctggt gacttgaatg atgttcggat gattcagtca agtagtggga
     4201 ttcactcctt tgaaccttcg gcaaatgata tcgcagccat ctatgatgca gatgtctttg
     4261 tttaccattc tcatacactc gaatcttggg caggaagtct ggatccaaat ctaaaaaaat
     4321 ccaaagtgaa ggtcttagag gcttctgagg gaatgacctt ggaacgtgtc cctggactag
     4381 aggatgtgga agcaggggat ggagttgatg aaaaaacgct ctatgaccct cacacatggc
     4441 tagatcctga aaaagctgga gaagaagccc aaattatcgc tgataaactt tcagaggtgg
     4501 atagtgagca taaagagact tatcaaaaaa atgcgcaagc ctttatcaaa aaagctcagg
     4561 aattgactaa gaaattccaa ccaaaatttg aaaaagcgac tcagaaaaca tttgtaacac
     4621 aacatacagc cttttcttat ctagcgaaga gatttgggct taatcaactt ggtattgcag
     4681 gtatctctcc tgaacaagaa ccaagtccac gacaactaac agaaattcag gaatttgtta
     4741 agacctataa ggttaaaacg atttttacag aaagtaacgc ttcttcaaaa gtagctgaaa
     4801 ctcttgtcaa atcaacaggt gtgggtctta aaactctgaa tcctttagag tcagacccac
     4861 aaaatgacaa gacctattta gaaaatcttg aagaaaatat gagtattcta gcagaagaat
     4921 taaagtgagg aaagaatgaa aatcaataaa aaatatctag caggttcagt ggcagtcctt
     4981 gccctaagtg tttgttccta tgaacttggt cgtcaccaag ctggtcaggt taagaaagag
     5041 tctaatcgag tttcttatat agatggtgat caggctggtc aaaaggcaga aaatttgaca
     5101 ccagatgaag tcagtaagag agaggggatc aacgccgaac aaattgttat caagattacg
     5161 gatcaaggtt atgtgacctc tcatggagac cattatcatt actataatgg caaggttcct
     5221 tatgatgcca tcatcagtga agaacttctc atgaaagatc cgaattatca gttgaaggat
     5281 tcagacattg tcaatgaaat caagggtggc tatgtgatta aggtagacgg aaaatactat
     5341 gtttacctta aagatgcagc ccatgcggac aatattcgga caaaagaaga gattaaacgt
     5401 cagaagcagg aacgcagtca taatcacaac tcaagagcag ataatgctgt tgctgcagcc
     5461 agagcccaag gacgttatac aacggatgat gggtatatct tcaatgcatc tgatatcatt
     5521 gaggacacgg gtgatgctta tattgtccct cacggcaatc acttccacta tattcctaag
     5581 agtgatttgt ctgccagtga attagctgct gcccaagcct attggaatgg gaagcaggga
     5641 tctcgtcctt cttcaagttc tagtcataat gcaaatccag ctcaaccaag attgtcagag
     5701 aaccacaatc tgactgtcac tccaacttat catcaaaatc aaggggaaaa catttcaagc
     5761 cttttacgtg aattgtatgc taaaccctta tcagaacgcc atgtagaatc tgatggccta
     5821 gtatttgatc cggcacaaat aacatatcgt acagtcaatg gtgttgctgt accgcacgga
     5881 gaccattatc actttattcc ttattcacaa ctgtcacctt tggaagaaaa attggctcgt
     5941 atgattgctg ttaagggaca aaatggtgca gtcttgccag gaatgcatta tttgaaacca
     6001 gcaccaaaac cgcaagttca accatcaact gagaaaaaac aaactgattt tgcagtagaa
     6061 caggttgtta gaaaagttgg agaaggctat gttgtggaaa ttgctggtgt cagtcactat
     6121 gtttttgcta aagacttggc taaggataag attgatgcta ttgaaaatct cttgtctaag
     6181 aaaactcaag agacacatgc tctagttgct aaaaaagaaa atgtcgctcc tcgtgaccaa
     6241 gaattttatg ataaagctta caatctgtta acccaggctc ataaagtctt gtctgaaaat
     6301 aaaggtcgta cttctgattt ccaagcctta gacaaattag cagaacgctt gaataatgaa
     6361 tcttctaata aagtaaaatt ggtagatgat ttattggcat tcctagcacc aattacccac
     6421 ccagaacgcc ttgg
//
LOCUS       DAVBCN010000095         6247 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVBCN010000095 DAVBCN010000000
VERSION     DAVBCN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.095"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(6..1220)
                     /locus_tag="VQ497_001858"
     CDS             complement(6..1220)
                     /locus_tag="VQ497_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2518133.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(1376..1669)
                     /locus_tag="VQ497_001859"
     CDS             complement(1376..1669)
                     /locus_tag="VQ497_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518134.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVKVIMICQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            1818..2270
                     /locus_tag="VQ497_001860"
     CDS             1818..2270
                     /locus_tag="VQ497_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET2518135.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(2305..3693)
                     /locus_tag="VQ497_001861"
     CDS             complement(2305..3693)
                     /locus_tag="VQ497_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2518136.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            3759..4721
                     /locus_tag="VQ497_001862"
     CDS             3759..4721
                     /locus_tag="VQ497_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET2518137.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(4973..5983)
                     /gene="ccpA"
                     /locus_tag="VQ497_001863"
     CDS             complement(4973..5983)
                     /gene="ccpA"
                     /locus_tag="VQ497_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HET2518138.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKVGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
BASE COUNT         1830 a         1450 c         1187 g         1780 t
ORIGIN      
        1 aagccctatc tacgatactg tttcaagaaa cgagtcatct tttcttggat ttgggctaac
       61 tcatcaacac gaggaaggta aacgatacgg aagtggtctg gttcctgcca gttaaagcct
      121 cgaccatgaa ccaagagaac cttttcctgc ttcaagaaat caaggacaaa ctgctcatca
      181 tcatcgatac ggtacatatt gcggtcgatt tttgggaaga tatagagccc cgccttgggt
      241 ttaacggcag acaaacctgg aatatcttga atggcattat agatgaaatt tctttgctcg
      301 tagattcgtc caccaggaag aagcaattca tcgactgatt ggtgaccccc caaggaagtt
      361 tgtacgactt gttgggccaa aacgttagag caaaggcgca tattggacag catattgagc
      421 ccttcgatat agcccttaac atgagtctta gggccagaca agaccatcca acccacacgg
      481 aaacctgcta tgcggtggga ttttgacaga ccattcatgc tgacacagaa gacatctggt
      541 gccaagctcg ccacaggcgt atgcacatgt ccgtccatta ccatgcggtc atagatttca
      601 tccgcaaaga taatcaaatc gttttgacgg gcaatctcaa taatctccag caagagttcc
      661 ttaggataaa gggctccagt tgggttattt ggattgataa ggacaattgc cttggtattg
      721 gaagtgattt ttgacttgat atcgtcaata tctgggtacc attccacagc ttcatcacag
      781 atatagtgaa cggcatttcc tccagctagg ctgacagccg ccgtccatag aggatagtct
      841 ggcattggca ctaagacctc atccccattg tccaaaagcc cctgcatgga catgacaatc
      901 aactcactga caccatttcc aaggtaaata tcatcaatat ctacgtttgg gaatttcttc
      961 agctggcaat actgcatgat ggccttacgg gctgagaaaa tccctttgga gtcagaatag
     1021 ccttcactat cacgcgcatt cataatcaag tcatgaatga cctcgtctgg cgctgtaaag
     1081 ccaaattctg ctggatttcc tgtattcaga cgtaaaatct tttctccatt cgctcgcatc
     1141 cgcatggctt cttccaatac aggaccacgg atatcataag caacatgctc taacttacta
     1201 gacttgttat attctttcat cttgtttcct caattcatca ggatagtaac attataccac
     1261 tagaaagaga atgcgacaag tttactgaaa tgtgttacta gtttcgtata gaagaaaaac
     1321 cttgctaaag gcgaggttaa gaacgtagag atagcaggca ttttgcaaag acacatcaca
     1381 taccaaaaga taaacctaga aaaagcaagc ctagaaagga gtcataaaga agattaaaca
     1441 tgaggaaaag cccccatatc atcaagtaat cccaacgccc acttcgtttt gcaactagaa
     1501 ataagattag atggtagagt acatgaaaga ctaaaaatcc aataaaactt ggataaagaa
     1561 gcggtaccag actctctaat tgacagatca tgatcacttt taccaagact gaaactagga
     1621 taattccata atacaggaaa tttgattttt gaaaagagtt tttcatcatt ctcctttcct
     1681 tctatccttt cttttatttt atcatatatt tatatagatt ttaagcccta atattctttt
     1741 gaaaaatact ttttttgctt tacttctata gtgaaaaggt ttacaattat agtaagaaaa
     1801 tttcaggagg tttcattatg gcacaacgtt accaaaatat catggtcgca atcgatggtt
     1861 ctaaggaagc ggacttggct tttgtcaagg gagttcattc tgctctacga aacgacgcta
     1921 aactcaccat cgcacatgtc attgacacac gcgctctcca aagcgtatcc acctttgatg
     1981 ctgaagttta cgaagaactc caagtcgacg ctgaaagtct gatgaaagag tacgaaaaac
     2041 gtgctaaaga tgctggggta gcagatgttc atatcgtcat tgaaatggga aatccaaaga
     2101 ccctgctagc acgtactatt ccagatgccg aggaagtgga cctcatcctc gttggcgcaa
     2161 ctggtctcaa cgcctttgaa cgcctcttgg tcggctcttc atctgaatac atactccgcc
     2221 atgctaaggt cgatttgctg gttgtgagag aacaagaaaa aaccttataa tcacaaagaa
     2281 aaggagcccc tagctccttt ttgtttacga tttatttctc tctttatggc gttcgtaagc
     2341 cttgagctgg cgctgcagtt cctttttaat agcaggttct ggagcatatt tttcttccca
     2401 attatctggt tttaagattt tatgggtcac tggatcaaaa tgagccttgc catctggaaa
     2461 aattttcccc atattggcct gatggacaat atcaaaaata cgttctgggt ccacccccat
     2521 caagacaaaa ctgccgtagg tgaagtaaag cgtgtcaatc aaggcatcca cttgccctat
     2581 caaatcttgc tgagcaggtg tcttcttggc tactttacct gctgccttat caagggcctg
     2641 atgaagttgc gatacagctt gaccaaaatc ttcttcagaa ggactggctg ctcgaacaaa
     2701 ctccaccaat tcttctattt taaaaccagc cctatgggtt gcaccctcta aatcccaagc
     2761 tcgaggttct tcttgggttc gttcatccat catgtggtgg aaggtcttga ccttattgaa
     2821 atgatagtca cggctgacaa agactttttc tgaagacaaa acaccaaaat gttccaaggc
     2881 cttgtggatc ccatcttgtt gattgctagt ggtaatgtgc ttggcagctt ccttgacact
     2941 gctactacca tttcccatag cgaccgacat accgacgcca gccagcattt caaggtcatt
     3001 atctgagtca ccaaaagcca tgacttggtt gaggtcaaag ccatattctt tcccaactcg
     3061 gcgaatgcct tctaatttag aatttccctg attgatgaca tccgatgcaa aaggattgct
     3121 acgtgtcaat ttcaagtctt caaaatcagc tgccgccttc tcagattctt ctggtgtcat
     3181 cagcatcaaa acttggtaga taggctgatt catcaggtga agcaggtcct cttccttttg
     3241 gggaacaacc ttgctgacca tgcgattaaa agactgactc accgtccgag ttaaaacaga
     3301 gggaacgaag cgactaattc gttgggaaaa agaacccaga ccaaaggaca tgattttaga
     3361 acccaacatg gcatccttgg tccctagggc aatctccgtg ccctcttttt tagcatagct
     3421 aattagatgg cgcaaatgta acttggaaat agggctcgtg aacaagactc tgtctttact
     3481 aaagatatac tggccattat aggttaccgc aaaatccaga tccaaatcgt ccatcaattc
     3541 cttaacaaaa aaaggtcctc gccctgtcgc tacgccaact agtacccctt gttctttgac
     3601 aatcttaatc gcgtccttag tggatttcaa aacactcttg cgattgttga ccaaggttcc
     3661 atcgatatca aaaaaaacag ctttgacttc catcctattc caatctcccc ttttgtgata
     3721 caatgattat accacatttc agaaagagtg agtaaatcat gcctaagaaa atccttgttt
     3781 tacatacggg tggaactatt tccatgcagg ccgatgcttc tggcgctgtt gtgacgagtt
     3841 cagataatcc catgaaccat gtgtccaacc cacttgaagg aatccaagtc cacgccttgg
     3901 acttttttaa ccttccaagt ccccatatca aacccaaaca tatgctggtc ctctaccaga
     3961 aaattaaaga ggaagcagat aactacgatg gagtggtaat cacacacgga accgatactt
     4021 tagaggaaac agcctatttc cttgatacca tggaagttcc ccatatgcct atcgttctaa
     4081 caggagccat gcgtagctcc aatgagctcg gtagtgatgg tgtttataat tacctaagtg
     4141 ctttacgagt ggccagcgat gacagggctg ctgacaaagg agttttggtc gttatgaacg
     4201 atgaaatcca cgctgccaag tatgtcacca aaacacatac gactaatgtc agcaccttcc
     4261 agactccaac acatggcccc ctcggtctca tcatgaaaca ggaaatcctc tacttcaaaa
     4321 cagctgaacc tcgtgttcgc tttgaccttg atcacataca aggtttagtc cctatcatct
     4381 cggcttatgc tggtatgaca gatgagctga ttgatatgct ggatttagaa cacttggacg
     4441 gtttgattat ccaagccttc ggagctggta atattcccaa agaaacggct caaaaattag
     4501 aaagccttct gcaaaaagga atcccagtcg ctctggtatc acgatgcttt aacggtattg
     4561 ccgagcctgt ttatgcctac cagggtgggg gcgtacagtt gcaaaaagca ggcgttttct
     4621 ttgttaaaga actcaacgcc caaaaagctc gcttgaaact cctcatcgcc ctcaatgccg
     4681 gactaacagg acaggctttg aaagactata tggaaggcta atactcttcg aaaatctctg
     4741 caaaccacgt cagagtcgcc ctaccgtatg tatggttact gacttcgtca gttctatcta
     4801 caacctcaaa aacatgtttt gagctgactt cgtcagtttc atctacaacc tcaaaacagt
     4861 gtgttgcgca gcctttaacc agccgcctag tccgctctat ggtattcatt aagtcaacat
     4921 ctcttgttta agagcacaaa atcaggaaat cttctcgatt ccctgatttt ttctatttac
     4981 gttttcgtgt tgagctacgt tctgtcaaac catgaggtaa gagaacttca cgttcttcca
     5041 actcttcctt atgcataatc ttggtcaaca tacgcatact aatggcacca aggtcataaa
     5101 gaggttgggc aatcgttgtc aagtttggac gggtaaagcg tgagatttgt gaatcatcac
     5161 tagtaataat ttcaaaatct tctggcacag aaacaccctt atcagccaaa ccgttcaaga
     5221 ctcctgctgc caactcatca cctgtcacaa ctgctgcagt tgcatttgat gaaatcaaac
     5281 gctctgctaa ggcgtaacca tcatcatagc tatatttaga ttcaaatacc aaaccctcac
     5341 tataagtgat tcctactttt ttcaaggttt ccttgtagcc aactaaacga accttaccat
     5401 tgatgtcatc cactagcgga ccgctaacga aagcaatacg ctcattttct ttagcaaggt
     5461 agctcactgc atcaattgtt gcttgcttat agtcaatatt gacacttggc aactggtgct
     5521 caacatcgac agttcctgcg agaacaatcg gagtacgtga acgcgaaaat tctgagcgaa
     5581 ttttatctgt caagtgataa cccatataga taatgccatc tacctgcttt gaaaagaggg
     5641 tattgacaac agaaacttct ttctcgttat cttcatcgct attagctagg acaatattgt
     5701 acttgtacat ttctgcaata tcatcaatcc ccttagccaa actcgaaaaa taaccattgg
     5761 taatatttgg aatcacgaca ccgacagtgg ttgtcttttt acttgcaaga ccacgcgcaa
     5821 ctgcatttgg acgataatcc aaacgatcaa ttacctctag cactttttta cgggtattct
     5881 cttttacatt tttattgcca ttgaccacac ggctgaccgt cgccatggaa acacctgctt
     5941 cacgagcgac atcataaatg gttactgtat catctgcatt cattcctttt cctgtccttt
     6001 ctatctcaca cattctttta caagtagagg tactgattga agctctatat ctacttacaa
     6061 aagtgaagat gtgaaaattt cgttttcata tttctactta ttccattcta tcactaattg
     6121 taaacacttt caagtgtttt ttgaagattg attgaaaaaa tttcatagaa aacctaggtt
     6181 tagctccttg ctaccacctt agactaaata aaaaggagga aactaagccc ttctaaagtt
     6241 atagtaa
//
LOCUS       DAVBCN010000096         6169 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVBCN010000096 DAVBCN010000000
VERSION     DAVBCN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.096"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            694..1713
                     /locus_tag="VQ497_001864"
     CDS             694..1713
                     /locus_tag="VQ497_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic biosynthesis protein"
                     /protein_id="HET2518139.1"
                     /translation="MSNTLKQLLLFREEFIKNILNGIDVDETVVIENDFVEELENFLK
                     ENDLLDFSNSIFFQLSGDKVIVNNIYPGYLAYFNRFLTFYPKIFAIKEVGEYIDFLNF
                     EKRIADMFYCWGFNANSRVLTAEKIIEIPNHQINAKYIKKVIKMSELLFGVDSNNRIR
                     LLNNRRELIKTIFQGSVIKSAIPALAATLDTISNSGSFQISLSKQIINNLVLSGKDKV
                     TIPRIELDNVVLSRQKIIVSVSYINDFLDLRFPDKIEYILNFLEGSGFNTKVMVYFGK
                     IVEEDKNYFVNLEKPQYFDFTSILFNKLFFKELNKFDYMVIEELIPNLDEDVMMTEYI
                     KEYTE"
     gene            1726..2829
                     /locus_tag="VQ497_001865"
     CDS             1726..2829
                     /locus_tag="VQ497_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518140.1"
                     /translation="MILSKVRCYIYNSKIRGKVLYELYEFINENKQLMDAINSIKVDS
                     SNGFSIQILHLGEQQTTVLLTDFFTKTLDLFKEFRVTNKSTLEQVKNQISILNSSGPK
                     MDLTNIKTDYSIVEEKLDWQSKLSKIYPNVEFIKIEKLKLILLCKYRNQIIDLEDDKK
                     RLLLLKLLYLTSKQFCYDEFKGAKFGCMSFFSHYQGYMSRLDSLDSDKFVSVKQRIVS
                     SISKLVEQYSDHIINLDRTLVDSLSDTLENQLFDDWDYILSEITTYSELLAKSGLLYN
                     SLDYKIDNVKKEVAKLSDFHQSLFKIEGFSDFFDSWQFQRYRSLVNQVYEIFSMLQVS
                     SVERTVLCGALSTIVFSKSNFNYQAFFEYLKDT"
     gene            2858..3766
                     /locus_tag="VQ497_001866"
     CDS             2858..3766
                     /locus_tag="VQ497_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518141.1"
                     /translation="MDYVDVILDEYGSVFLIKDHIKLKINNLELYRYFSSFGMSHLQF
                     RKEELPGKLKQFDFEKNKSDSYFSKKSSLIDFIKRNYFYDIKLYSDNPKNPIESWKNK
                     TEKIKIAKSTEILWSEVANFSSISKSPYYCLLYNLKNTENYILIISKTYFSAIQKMVS
                     YNLNNYFELEDVCCKSIVSKRELIENSININKSKMKLLNVCEGSKGYTRRVFKYNNIF
                     VGQLEFHSNIIFSEIALNELSIVKLLDSVESVLSEQLFGKLNWDNLFVVLSDDFNDYL
                     DINESQKYYSNGSLRGLELGIFMGGV"
     gene            3768..5666
                     /locus_tag="VQ497_001867"
     CDS             3768..5666
                     /locus_tag="VQ497_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HET2518142.1"
                     /translation="MRIIEIVDVEDQLLQLLSAHTNIDIRAQELSTLDPDSKVDLIIY
                     LKSRRDIENINNIIIKSPNRNIIVIISAFDIIYALYFPKSTFRPCLNCLLLKLKDNNP
                     EHNLLLFLKYGTLPSKHFNLDNRLKSNLILFNEKLDPKVEEYSIEGVNTTNFTFHFSC
                     SCENSGYDMSERMADILLGEGKKLSPKNYRLKKSEHLFDFTRYLDRNFGFFHHSYDEK
                     ISEYAPLHAVEINVDAGYESGWGRTFMTEAATKSAFLESLERYCNSRNRRYKKLIRGC
                     FSDLSKKYNTLNPVDFCLSNKEYRDSSYKFHDFDSEKNYDWIFGKNITTGKICLLPSQ
                     LVFYKDFSKSEENERFVYETSNGCALGSSIEEAIFYGILEIIERDNFLLTWYLKEEVK
                     QLSWESLPSRLRIINERLKEDNCELKLFNITKEVGIPVIWALYINHNENASIRSFSAA
                     GCHINPLSAIESAIFEVITSVPTFERLIEVRNKESFIEEIQNNFSLIRDIDDHILYHA
                     HADYKNKMPFLFKNNYSEYQNEFDENRIFNTYSHATLTSDLQALIKIIEKKFGDIYVV
                     NISDKVSEKFGLYCVKVLIPGLLNMSFGHSNRRVNIERIKNYVDYRGGNVNSITLNSI
                     NHLPHPFP"
     gene            5690..>6169
                     /locus_tag="VQ497_001868"
     CDS             5690..>6169
                     /locus_tag="VQ497_001868"
                     /inference="COORDINATES: protein motif:HMM:NF025394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HET2518143.1"
                     /translation="MKRDWKSKHLFYHDVQQFDKLVIYLSKFMDKLVNQNQIEKYFYI
                     RYWEGGPHIRLRYLVRDTQNFETIDSMINKYAYKFWENNPPMKLLNKTDYKKIYNESI
                     DNNGKMDRKLRKNATIENIAYIPEIERYGGEEALIVSEDLFCICSQVSTALLNVVDNY
                     "
BASE COUNT         2306 a          673 c         1076 g         2114 t
ORIGIN      
        1 tagaacaaat cgatcaggat agtcaaatcg atttctaaca atgttttaga agcagaggtg
       61 aactattcta gtttcaatcc actatagtag ctcagaagtc ggtacttaaa cgtgctatat
      121 caaaaccagt ccttgaaaaa cgtggattgg tttcgtgttt ggattattac cttgaacgac
      181 atgcgttaaa agttagttga accgccgtat gccgaacagc acgtacggtg gtgtgagagg
      241 ggctagagat tatcccctac tcgattgtct atagctatag gggtaaagtt attagagagg
      301 ttgctagttt atagagacat cttgatttgt aaaaagttaa attttagaca attatcccct
      361 ttaaaaaatc tattaaacca tagctagttg aagtaaattt ttatagaaca taattttatt
      421 tatcaagaca ttgtagagtt gaggcaaatt gaaaaagaaa gtttaaaagt tgataataag
      481 tcaataagaa agctattaat tctaacgaga ttatttgata gtaatttgtt ttttcagcta
      541 ctattcggac aatgttatta taataaacac aggagagaac accagaaggt agggacatca
      601 gaagaaatag ttcaggatat agttaacata atcatcaaag aagctcctag actcgctaat
      661 gttaatttgg aaacatatta tccagatgaa tatatgtcaa atacattgaa gcaattacta
      721 ctttttagag aggagttcat aaaaaatatt ttgaatggga ttgatgtgga tgaaacagta
      781 gtaatagaaa atgattttgt agaagagttg gagaactttt taaaagagaa tgatttgcta
      841 gatttttcaa atagcatatt ttttcagtta tctggagata aagtaatagt gaataatatt
      901 tatccaggat atttagcata ttttaatagg ttccttacat tttatccgaa aatattcgct
      961 attaaagaag ttggagaata tattgacttt ttaaattttg aaaagagaat tgcggatatg
     1021 ttttattgtt ggggatttaa tgcaaattct agagtgttga cagctgaaaa aataattgaa
     1081 atcccaaatc accaaatcaa tgctaagtac ataaaaaaag ttataaaaat gagtgaactc
     1141 ttgtttggtg ttgatagtaa taacagaatt cggttgctta acaatagaag ggaattgata
     1201 aaaactattt tccaaggttc tgtaattaag tcagcaattc ctgctctagc tgctactttg
     1261 gatacaatca gtaacagtgg atcatttcaa atttctttat caaagcagat aattaataat
     1321 ttagtattaa gtggtaaaga taaagttaca attcctagga tagagttaga taatgttgtt
     1381 ctttcaagac agaagattat tgtatcagtt tcgtatataa atgatttttt ggatttgaga
     1441 tttccagata aaatagaata cattttaaat tttttagaag gatctggttt caatactaaa
     1501 gtaatggtct actttgggaa aattgtagaa gaagataaga actattttgt taatcttgaa
     1561 aaacctcagt attttgattt taccagtata cttttcaata agttattctt taaagaatta
     1621 aacaagtttg attacatggt tattgaagaa ctcattccaa atcttgatga agatgtaatg
     1681 atgacagagt atataaaaga gtatactgaa taggagcaat ctattatgat tttaagtaaa
     1741 gtacgttgtt acatatataa ttctaaaatt agaggtaaag ttctgtatga attatatgaa
     1801 tttataaatg aaaataagca attgatggac gcaattaatt caattaaagt tgacagtagt
     1861 aatggtttta gtattcaaat attacattta ggagaacagc aaacaactgt cttattaaca
     1921 gattttttta ctaaaacttt agacttgttt aaggaattta gggttacaaa taagagtact
     1981 cttgagcagg tgaaaaatca aattagcata ctaaattctt ctggtccaaa aatggattta
     2041 acgaatataa aaacagatta ctcaatagtg gaagaaaaat tagattggca aagtaaatta
     2101 tctaaaattt atccgaatgt tgaatttata aaaattgaaa aattaaaatt gattttgttg
     2161 tgtaaatata gaaatcaaat tattgactta gaggatgata aaaaaagatt gttactattg
     2221 aaattattat atttgacttc aaagcaattt tgctatgatg aatttaaagg agcgaaattt
     2281 ggatgtatgt catttttttc acattatcaa ggttatatga gcagattaga ttcactcgac
     2341 tcagataaat ttgtttcggt taaacagagg attgtttcct ctatttctaa attagttgag
     2401 caatattcag atcatattat aaatctggat agaactttag ttgattctct aagtgatacc
     2461 cttgagaatc agctatttga tgattgggat tatatattaa gcgaaataac tacatatagc
     2521 gaattattag caaaatcagg attgttatat aattcgctag actataagat tgataatgta
     2581 aaaaaagaag tggccaagct aagtgacttt caccaatctt tatttaaaat tgaaggattt
     2641 tcagattttt ttgatagttg gcaatttcaa agatataggt ctctcgtgaa ccaagtttat
     2701 gaaatttttt caatgttaca agtaagtagt gtagaaagaa ctgtgttgtg cggagctcta
     2761 agtactattg tctttagtaa gtcaaatttt aactatcaag ctttttttga gtatctaaaa
     2821 gatacgtaga attcgatttt aaaggaggct gaaatatatg gattatgtag atgtaatatt
     2881 ggatgagtat gggagtgttt ttctgataaa ggatcacata aaactaaaaa ttaataatct
     2941 agaactatat cggtatttta gtagctttgg aatgagtcat cttcaattta gaaaagaaga
     3001 gcttccagga aaattaaagc agtttgattt tgaaaaaaat aaaagtgaca gttatttttc
     3061 taagaaaagt agtctgattg attttattaa gagaaattat ttttatgata ttaagttata
     3121 ttctgataat ccgaaaaatc cgatagagtc ttggaaaaat aagacagaaa aaattaagat
     3181 tgctaaaagt acagaaattc tttggagtga agtagcaaat ttttcgtcaa tttctaaatc
     3241 tccttactat tgcttgttat ataatttaaa gaatactgaa aattacatat tgataatcag
     3301 taagacatat ttttcagcca tccaaaaaat ggtttcgtat aatctaaata attattttga
     3361 attagaagat gtttgttgta aaagcatagt gagcaagcga gaattgatag aaaattcaat
     3421 taatattaac aaaagcaaaa tgaaactgct aaatgtttgc gaaggtagta aaggttacac
     3481 aagaagagta tttaagtaca ataatatttt tgtgggacaa ttggaatttc actctaacat
     3541 aatcttttca gaaattgctt taaatgaatt aagtattgtt aagcttttag attctgttga
     3601 aagtgtttta tctgagcaac tatttggaaa attaaattgg gataatttat ttgttgtttt
     3661 atcagatgat tttaatgatt atctggatat aaatgaatct caaaaatact actcaaatgg
     3721 ttctttgaga ggactggagc ttggaatctt tatgggaggg gtttaaaatg agaataattg
     3781 aaattgttga tgttgaggac caacttttac aacttttaag tgcacatacc aatattgata
     3841 ttagagcaca agagttatca acacttgacc cagattcaaa agttgatttg atcatatact
     3901 tgaaatctcg acgtgatatt gaaaacataa ataatattat tattaaatca cctaacagga
     3961 atatcattgt tataataagt gcttttgata ttatatatgc attgtatttt ccaaaatcaa
     4021 catttagacc ttgtttgaac tgtctattat taaaacttaa agataacaac cctgagcata
     4081 atctattatt attcctgaaa tacggaacat tacctagtaa acattttaat cttgataata
     4141 ggttaaaatc aaatttaata ctatttaatg agaagttaga ccctaaggta gaggagtata
     4201 gtattgaggg ggtaaatact acaaatttta ctttccattt tagttgtagc tgtgaaaata
     4261 gtggttacga tatgtcggaa agaatggcag atattctact tggtgaagga aaaaaattat
     4321 ctcctaaaaa ttatcgttta aaaaagagtg agcatctatt cgattttact agatatctag
     4381 atagaaattt tggatttttt catcattcat atgatgaaaa aatctccgaa tatgcgcctc
     4441 tccatgcagt agagattaat gttgatgctg gttacgagtc aggctgggga aggactttta
     4501 tgacagaggc tgcaacaaaa tctgcttttt tagagtcatt agagagatac tgcaactcta
     4561 gaaatagacg ctataaaaaa ttaattagag ggtgtttttc tgatttatct aaaaagtaca
     4621 atacattaaa tcctgttgat ttttgtcttt ctaataaaga atatagagat agttcctata
     4681 aatttcatga ttttgattct gagaaaaatt atgattggat atttggtaaa aatataacta
     4741 ccggaaaaat ttgcttattg ccctcgcaac tagtatttta taaagacttt agtaaaagcg
     4801 aagaaaatga aaggtttgtt tatgaaacat caaatggatg tgctttgggg agtagtattg
     4861 aggaagctat tttttatggg attttagaga ttattgaaag agataatttt ttacttacat
     4921 ggtatttaaa agaagaagta aaacaattat catgggaatc tttaccatct cgcctacgta
     4981 ttattaatga aagactaaaa gaagataact gtgagctcaa actttttaat ataactaaag
     5041 aagtgggcat accagtaatt tgggcattgt atataaatca taatgaaaat gctagtattc
     5101 gaagtttttc agctgcaggc tgtcatatta atcctttaag tgcaattgaa tcagcaattt
     5161 ttgaagtaat aacttctgtg ccaacttttg aaagattaat tgaagttaga aataaagagt
     5221 catttattga ggaaattcag aataattttt ctttaatacg agatattgac gatcatatat
     5281 tgtatcatgc tcatgcggat tataaaaata aaatgccctt tttatttaaa aataattata
     5341 gtgaatatca aaatgaattt gatgagaata gaatttttaa tacttattca catgcaacgc
     5401 ttacatcaga tttacaggca ctaataaaaa ttattgaaaa aaaatttgga gatatatatg
     5461 tagtgaatat aagtgataaa gtttccgaaa aatttggact ttattgtgtt aaagtgttga
     5521 ttcctggact tcttaatatg tcatttggac attcaaatcg gcgtgtaaat atagagagga
     5581 taaaaaatta tgtagattat agagggggaa atgtaaatag tattacattg aattccataa
     5641 accatcttcc ccatccattt ccataagatt atatataaag gaataataaa tgaaaagaga
     5701 ttggaaatca aagcatttat tttatcatga tgttcaacaa tttgataaat tggtaatcta
     5761 tttatcaaaa tttatggata aattagttaa tcagaatcag attgaaaaat atttctatat
     5821 tcgttattgg gaaggggggc ctcatattcg attaagatat ttggtgaggg atacacaaaa
     5881 ttttgaaaca attgatagta tgataaataa gtatgcttat aaattttggg aaaataatcc
     5941 tccaatgaag ttattaaata agaccgatta taagaaaatt tataatgaga gtattgataa
     6001 caacggaaag atggatagaa aattgaggaa aaatgcgact attgaaaaca ttgcctatat
     6061 accggaaata gagagatatg gcggggaaga agctctgata gtaagcgaag atctattttg
     6121 tatctgtagt caggtttcaa ctgcgttact taatgtggta gataactat
//
LOCUS       DAVBCN010000097         5942 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVBCN010000097 DAVBCN010000000
VERSION     DAVBCN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.097"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            406..1311
                     /locus_tag="VQ497_001869"
                     /pseudo
     CDS             406..1311
                     /locus_tag="VQ497_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512061.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            1356..1796
                     /locus_tag="VQ497_001870"
     CDS             1356..1796
                     /locus_tag="VQ497_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2518144.1"
                     /translation="MNFVLSSLSEGLLWSIMAIGVYLTFRILDIADMTAEGAFPMGAA
                     IVVSQIQAGANPWLATLLALLAGMVAGLISGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGAVFSLSLVCLLLVWF"
     gene            1805..2233
                     /locus_tag="VQ497_001871"
     CDS             1805..2233
                     /locus_tag="VQ497_001871"
                     /inference="COORDINATES: protein motif:HMM:NF014684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518145.1"
                     /translation="MKTEIGLVLRSTGDNIPMSEANGVNVNTMKIVGYMISTGLIALC
                     GSLFAQNDGFSDVTSGTGTIVVGLSSEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYE
                     IPNLDQNLVRLFNAILLALVLFAPALQKRLKIRGLKLRNE"
     gene            2246..3049
                     /locus_tag="VQ497_001872"
     CDS             2246..3049
                     /locus_tag="VQ497_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2518146.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKHLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMAALVKLKLLLLDEHTAALDPKTSQMVMDLTQKIVEYHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(3082..3792)
                     /gene="treR"
                     /locus_tag="VQ497_001873"
     CDS             complement(3082..3792)
                     /gene="treR"
                     /locus_tag="VQ497_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HET2518147.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            3977..>5942
                     /gene="treP"
                     /locus_tag="VQ497_001874"
     CDS             3977..>5942
                     /gene="treP"
                     /locus_tag="VQ497_001874"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HET2518148.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGNDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
BASE COUNT         1657 a         1106 c         1382 g         1797 t
ORIGIN      
        1 cctgaaagaa ttttcgaaaa attttaaaaa attttgaaat attattgaca ggtgatgaaa
       61 aaggtggtag gataggaagg atcaaaaaag aaagcagaaa aagaaatgaa taagagacaa
      121 gccgtaacta tgaatcaaaa cttttacttt agctacttta gatagtgttt gtagacatgt
      181 caccatggat gcaaacagtt caagcaattt gtttgtatct atgtggctaa gatttttgat
      241 tgtggtccat atagatgaat atctaaatgg accagctaag ttgtaacttg ttaggttatt
      301 tcaagtaccc gtttagatag tatctaggcg tttttttgtt ttatcttttc tgagaaggag
      361 tttcattatg aaagttgttc gaaaatcact agccccctct tggtagtggg gatcctcttg
      421 acttctctga tcagtttgca tcagttgaag gcagataaga aaagagatgt ctttcgtatc
      481 ggtatttcgc agtttattac ccaccagtcc ttagacgcta ctagagaagg atttgtggat
      541 gagctggcca aacaaggcta tgttgagggg gaaaatattg agattgattt gcaaaatgca
      601 cagggagaac aaagaaatct aaaaacgatt tcccagcaac tagcagaatc cagcgatgtc
      661 gttctagcca tcgcaaggcc ttctgctcag agcttggcca atacgacgca aacgacacca
      721 gttatctttt cagctgtaac agacccagtc agtgccaagt tggttgagtc aagagaacat
      781 cctgggggca atgtaactgg gacaagcgat cagtcatcag atgccatttc aacccaaatc
      841 aatttgataa agaaagtgtt gctaaaggct aaaacgattg gaatcctcta tactcagagc
      901 gagcccaatt cggttgttta aaaggatgaa gctaagcgat ttctgaaaga aaaaggcttt
      961 accgttgttg aaaaaacaat cttggacagt aataacgtca aggcggcagc agagagtttg
     1021 atggcagagg tggatatggt ttttgtccca actgataata tcatttcgtc aaccatggaa
     1081 acagttaagc aggtttctat taaacacaag gttccagtat tcggtggttc aacagaaatg
     1141 attgctgttg gtggcttgta taactacgga accaattatg aagaattggg aagacagaca
     1201 gctcgcatgc tgattcgtgt tttaaaaggt gaggaaccag aaaatatagc agttgagttg
     1261 cctgaaaaac tggaattgca tactaatcaa gaaatggcag atgcattgtg aattgatatt
     1321 agtaagttag aaggcaagga ataaggagga ttaagatgaa ttttgtgtta tctagtttat
     1381 cagaaggttt gttgtggtcg attatggcaa ttggggtcta cttgactttc cgtattttgg
     1441 atattgcgga tatgactgct gagggagcct ttcctatggg ggcagcaatc gttgtatcac
     1501 agatacaggc aggggcaaat ccttggcttg cgactttact tgctttgctg gcaggtatgg
     1561 tagcaggtct tatatcagga atgctccaca ccaagatgaa aatcccagct ctcttgacag
     1621 ggattgtgac cttgacaggg ctctattcaa tcaatattaa aatcatgggc agtgtaccca
     1681 atctttcctt gggagattct gcaaccgtct ttaaacagtt ggcgagcttg ggtctgacaa
     1741 atgaaggagc tgttttctca cttagtttag tctgtctctt acttgtttgg ttttgaccct
     1801 tttgatgaaa acagagattg gtttggtctt gcgttcgact ggggacaata tcccgatgag
     1861 tgaggccaat ggagtcaatg taaacaccat gaagattgtt ggttacatga tttcaaccgg
     1921 tttgattgcc ctatgtggtt ccttgtttgc ccaaaatgat ggattttcag atgtgacttc
     1981 tgggacagga accattgttg ttggtttgag ctcggaagtc ttgatacacg acttgaccat
     2041 tggaggccgc ttattatcca tcggaatcgg tgctattgtt taccgtttga ttattttaaa
     2101 tatctatgaa attccaaatc tagatcaaaa cttggtacgt ctctttaatg caatcttgct
     2161 cgctttggtt ttatttgcac cagcattgca aaagagatta aagattcgtg gtctgaaatt
     2221 gagaaatgaa taggaggaga aagttatggc aagtttgtta acacttgaaa atattcacaa
     2281 gacatttgaa gcagggacgg tcaatgagaa ccatgtcctc aaaggattag atttagaggt
     2341 tgaagaggga gattttatct ctgtgattgg tggaaatgga gcaggaaaat ccactttgat
     2401 gaatatcttg gctggtaatc tatcggtgga cgaaggggac cttctactgg cagggaaatc
     2461 cattaaacat ctgagtgtca gaaagagagc caaggatatt gcccgtgttt ttcaagatcc
     2521 taagatggga acagcttctc gtttgacgat tgaggagaat atggctattg ccttgagacg
     2581 tgggcaaaag agagggcttg gttggggcgt gaaggagaag gatagaatcc agttccaaga
     2641 ggccttgaag gagttgaata ttggtcttga aaaccgctta aaagtagata ctcaatatct
     2701 ctcaggagga caaagacagg ctttgaccct agtcatggca gctctggtga aactcaaact
     2761 tttgcttttg gatgaacata ctgcggcact tgacccgaaa accagtcaaa tggtgatgga
     2821 cttgacgcaa aagattgtgg agtaccatca gtggacgact ttgatgatta cccatgatat
     2881 gaatcatgcg attgagtacg gcaatcgtct cattatgctc tatcaaggca agatagtggt
     2941 ggatgtcaag ggagaagaga aaaagcatct gacagttgaa gaccttatgc atctcttcca
     3001 gaaaaatagt ggccaaagtc tggtcagtga tgaattagtt ttgggataaa gaaaaaggct
     3061 gagatctggt gtctcagtct tttatttctt gcgttttgca aaatctacaa atctaaattt
     3121 ttctaactta tggcgacttt cggtaaactg aaattggcgc ccgtcttgga gatagacttt
     3181 tgatttaatc gaaacgacat aagggtcttt gccaatgtcc atgagaatct tgtctcggtc
     3241 gcttgagtgg tcaatggtga tttccttctg agcataatca ataaggagtt tgaggccatt
     3301 ttctatataa gaatagatag actgctcagc aatttggcga gtgagatttg ggatgagttc
     3361 catatccaga tagtccgtat ccagaacgga taccagatca tccaccacac gctggcggac
     3421 caccttccaa accatccgaa actctgggaa accggttatc agtgaggatt ttttatcaat
     3481 aataatcttg tccagactga ccacgttggt tttagagcgc agtccaagtt ctttaactag
     3541 ttcttggtag ctggttaggt tggatacagg gaaattgacg gtttcttctt tgacgacttg
     3601 agaaccttgc cctcttatct ttttgatcaa tccttcctct tggaggagag acagggcctt
     3661 tcggacggta tcacgactca cttgatattg ctccataagg tcgtgctcgc taggaaggaa
     3721 atctccgaca gcgtaagtct cgttttgaat ggtttcttgg atttgcttaa ataattgttg
     3781 gtatttcttc attttgcctg cttaaatagg tttttagtct agtttaatga acacctgtat
     3841 ctattttacc atacaagtgc tagaaaattt acaaaaaaac acccttttta taccaaacaa
     3901 gttgcagaca agtttggtat cgtttacaat atacttatca aatcaaactt gctttgacaa
     3961 gtataaggag aatcaaatgg gaaaatttga acaagaagcc aaagatctgc ttcaggcaat
     4021 cggaggcaaa gaaaatgtga ctgccgtaac tcactgtgca acacggatgc gctttgtttt
     4081 aggaaatgat aagaaggcta atgttaaagc tatcgagtca attccagctg ttaaaggaac
     4141 ctttacaaat gcaggtcaat ttcaggtaat cattggaaat gacgtgccca tcttttataa
     4201 tgattttaca gctgtttcag gtattgaggg tgtttccaaa gaagcagcca agtctgcagc
     4261 taagagtaat caaaacgtgg tccaacgtgt catgaccact ctggcggaga tttttactcc
     4321 gattattcca gccttgatag tcggaggatt gatcctcggt ttccgtaatg tcttggaagg
     4381 tgtccattgg tcgatgttgg atggcaagac catcacagaa tcctctcagt tttgggcagg
     4441 tgttaatcat ttcctctggt tgcctggtga agctatcttc cagttcttac cagtagggat
     4501 tacttggtct gtttctcgta agatgggaac cagccaaatt ttgggaattg ttctcggaat
     4561 ctgtttggta tcgcctcagt tgctcaatgc ctatgcggtt gcttcaacgc cagcagctga
     4621 tatcgcggca aactgggttt ggaattttgg ctattttact gttaatcgta tcggttacca
     4681 agcccaagtt atcccagcct tgcttgcagg tttgagtcta tcttatcttg aaatcttctg
     4741 gcgcaagcat atcccagaag tcatttctat gatttttgta cctttcttgt cattgattcc
     4801 agccttgatt ttggctcata ctgttttggg accaatcggt tggacaattg gacaaggact
     4861 ttcatcagtt gtcttggcag gtttaactgg tccagttaaa tggctcttcg gtgcaatttt
     4921 tggtgccctc tacgctccat ttgtcatcac aggtctgcac catatgacca atgccattga
     4981 tacacaattg attgcggatg ctggtggcac tgccctatgg ccaatgattg ctctttctaa
     5041 tattgctcaa ggctcagccg tgtttgccta ttatttcatg catcgccatg atgagcgtga
     5101 ggctcaggtt tcacttcctg caaccatttc agcctatctc ggtgttacag aaccagctct
     5161 ttttggggtt aacgtaaaat atatttatcc atttgttgct gggatgactg gttcagccct
     5221 tgcaggcatg ttatccgtta cttttaatgt aactgcggct tctattggta tcggtggttt
     5281 gccaggtatt ctctctattc aacctcaata catgctgcca tttgcaggaa ctatgctagt
     5341 tgcgattgtt gttccaatgc tcttgacttt cttcttccgc aaggctggtc tctttacaaa
     5401 aacagagggc gatacgaact tgcaggcaga attcgttgct caagaagaag cagaatttgt
     5461 gaaccatgaa ccagtagaac ttacttcggt agaaattatc agcccactaa ctggccaagt
     5521 gaaagaattg agtcaagcga cggatcctat ttttgcatca ggtgtcatgg ggcaaggtct
     5581 agtcattgaa ccaagccaag gtgagttgac ctctccagtt aatgggacag tgacggttct
     5641 tttccctacc aagcatgcca tcggcattgt ctctgacgag ggagttgaat tgctcatcca
     5701 catcggtatg gatacagtag gtcttgatgg caaaggtttt gaaagtcttg tagtccaagg
     5761 agatcacgtt acagttggtc agcaactgat tcgttttgat atggatgtca ttaaggctgc
     5821 aggtctggtg acagaaactc ctgttatcat caccaaccaa gatgcttata cagcgactat
     5881 tcccggaact tatccgacaa cgattcaagc tggagcatct ctcatggtcg ctacacgaat
     5941 ct
//
LOCUS       DAVBCN010000098         5860 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVBCN010000098 DAVBCN010000000
VERSION     DAVBCN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.098"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(410..802)
                     /gene="rpsI"
                     /locus_tag="VQ497_001875"
     CDS             complement(410..802)
                     /gene="rpsI"
                     /locus_tag="VQ497_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET2518149.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVIGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(821..1267)
                     /gene="rplM"
                     /locus_tag="VQ497_001876"
     CDS             complement(821..1267)
                     /gene="rplM"
                     /locus_tag="VQ497_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET2518150.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            1623..1940
                     /locus_tag="VQ497_001877"
     CDS             1623..1940
                     /locus_tag="VQ497_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HET2518151.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(2090..2902)
                     /gene="folK"
                     /locus_tag="VQ497_001878"
     CDS             complement(2090..2902)
                     /gene="folK"
                     /locus_tag="VQ497_001878"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET2518152.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQVNLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(2945..3499)
                     /gene="folE"
                     /locus_tag="VQ497_001879"
     CDS             complement(2945..3499)
                     /gene="folE"
                     /locus_tag="VQ497_001879"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET2518153.1"
                     /translation="MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(3480..4802)
                     /locus_tag="VQ497_001880"
     CDS             complement(3480..4802)
                     /locus_tag="VQ497_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET2518154.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQDTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCRPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(4804..5778)
                     /gene="folP"
                     /locus_tag="VQ497_001881"
     CDS             complement(4804..5778)
                     /gene="folP"
                     /locus_tag="VQ497_001881"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET2518155.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGANLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEELMEAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
BASE COUNT         1622 a         1368 c         1127 g         1743 t
ORIGIN      
        1 taaactcttc acacaagagt tctagcttcc ccattctatg gaatcttgca ttatccataa
       61 tatagtagat tgaaactaga atagtacacc tctacttcta aaacattgtt agaattcgat
      121 ttgactatcc tcggtttgtc ctattcttat ttcaccttac tataaaaata aggtaaactt
      181 ttcaaaaaaa cacgaaaaaa gcacccataa ggtaaacttt tgagtgcttt gaaacgttga
      241 tataatcgta ttgattaacg ttttgaggca actgaccatg tcaggttgca gtcacatttg
      301 taagcgacta aagtcgcaac aactgtgtca attgcaccaa tttagtaaga aagtataaaa
      361 aaagaacacc ccgaaaggtg ctctgtataa gtatagtaat tctttcgaat taacgtttac
      421 taaattgtga tgctttacga gctttcttaa gacctggttt cttacgttca actttacgtg
      481 agtcacgtgt aagaagtcct gcgcgtttca atgaatcgcg gaagtctggg tctacttgaa
      541 gaagggcacg agcgataccg tgacggatag ctcctgattg accagcgtat ccaccaccta
      601 taacgttaac gaaaacgtcg tatgaaccta cagttgaagt aactgcgaat ggttggttga
      661 tgacaagacg aaggtcagcg tgtgggatgt actcttcaac atcttttttg ttaacagtga
      721 ttttaccagt tcctggaaca aggcgaacgc gtgcaacagc gtttttacga cgtccagtac
      781 ctgcatattg tgcttgtgac atactttatt gttcctttcc ttagataagt cctgaaatgt
      841 caagaacttc tggttgttgt gcagcgtgag tgtgctcagc tccaacaaat actttcaact
      901 tcataccttg agcgcgtcca agtgtattgt gtggaagcat acctttaact gatttctcaa
      961 tcaaacgtac tgcattttta gaacgaagtt cacctgcaga gatttgtttc aatccacctg
     1021 ggtggtttga gtgagtgtag tagattttat cagttgcttt tttaccagtc aatttaactt
     1081 tttcagcatt gataacaatc acgaagtcac ctgtatcagt gtgtggtgta aatgttggtt
     1141 tgttttttcc gcgaagtacg ctagcaacta ctgcagaaag acgtccaagt ggtacatcag
     1201 ttgcgtcaac tacgtaccat ttacgttcaa cttggcctgg tttagccata aatgttgttt
     1261 tgttcatgat ttctcctata tatagttcga tttttgttta cggtgatggg tgttccttcc
     1321 catcgaaaag tatttggaag gttccggggc ctttcaaatg gggtaaacaa taccgcctac
     1381 tatcatacca aattttaccc ctaaaagtca atagatatga aaaatatttt tcggtaactt
     1441 ctcaaagtaa ctgaaagtta tataatcaag ttaatttggg aatttaccta ttaacttcgg
     1501 tagattttta ggaatttttt ccctatttct ccctgtaact aaaatccttt tcaaaaaatc
     1561 accttttctt gatttttaat cctatttgct gtatgataag ggaaaagaaa ggggacagag
     1621 atatggcttt taccaatacc cacatgcgat ctgctagttt tggcattgtt accagcttgc
     1681 ctgatgacat cattgactct ttttggtata tcatcgacca tttcttaaaa aatgtctttg
     1741 aattggaaga agaactcgag tttcaattgc ttaataacca aggaaagatt accttccact
     1801 tttcaagtca acacctccct acagccattg attttgactt taaccatcct ttcgaccctc
     1861 gttatccccc aagagtactg gttttagaca tggacggtag agaaactatc ctcctcccag
     1921 aagaaaatga cctattttaa tactcaatga aaatcaaaga gcaaactagg aagctagccg
     1981 caagctgtac ttgagtacgg taaggcgacg ctgacgtggt ttgaagagat tttcgaagag
     2041 tataaaaact ctagccttca gttgcaagta actgaaaact agagtttttc tattttttca
     2101 aagcatcata caagttgcgg atcggttgtt ttaatatcgg atggataaaa tgaggcgcaa
     2161 tttcctgtaa ggactcaagg acaaaaaggc gttccgctat gtaaggatga ggcaatatga
     2221 ggtcgtctgt ataaaggatc tggtcctcca caaagagcaa gtccaaatca atcaaacgag
     2281 gtccccaatg cacttctctc acccgtccca gctctgactc aatggctaac aaggtttcta
     2341 acaagtcttg tgctggtagc caggtttcca cctcaaccac ttgattggca aagctatcct
     2401 gctccactcc accccaaggc tccgtcgcta agacactgga ctctttgaga atatggatgc
     2461 cacgagctcg cagtttgtca atggcttgct tcaagtttac ttgtttatct cccatattgc
     2521 ttcctagggc gataaaggct cgttgcttgc ggcgatgaat ggttaccgag caagtatcta
     2581 gtgacaaatg caccggtgcc caaggttttt tcagttccaa cttcatttct tggacaagag
     2641 gataagactc aaaggtacgt tccaccagtt tataggctac cgtttcaatc aaatcttcac
     2701 tcgtttcctg aaaccaagtc gtccactgct gacacaattc tccgtaatgg acagaggctg
     2761 ttaaatccaa gtctgtagct gccttggtca tatcatagga taggatggct gaaacgacaa
     2821 atttctgccc caattctttc tcactaggaa aaagaccatg ataggcaaac atttccaaat
     2881 ccttaatctg cagttgatcc ataatgattc ctttctagaa aaatccgctt gaagcggatt
     2941 ctttttatag ccccattaaa cgataagctt ggtcgcggag gtccttatct gtttcaaata
     3001 gaccacgagc tactgtcgtc aaggttgcag tgcctggttt tctaacacca cgcatactca
     3061 tacacatatg ttccgcctca atgacaacaa aggctccttt agcacctaga tagtccatca
     3121 aggcatcggc cacttcgata ttcaaacgtt cttgaatttg tggttttttc gaataaactt
     3181 caaccgtacg ggctagctta gacaagcctg ccacacgacc atctggaatg taggcaatgt
     3241 gcgctctacc ataaaatggc aagaagtggt gttcacacat ggtatggaaa aagatatcct
     3301 tttctaccac catattatcg tcaataattt caaaggattt tgacaaatgt tcctctgctg
     3361 tttgaccaag acctgaaaaa atctcttgat acatacgggc tacacgagca ggtgtttcct
     3421 gcaagccctc gcgattagcg tcctctccta cagcctcgat aatcattttt acagctgctt
     3481 caatcttttg tgtatccatt ctcgttcttc ctctccatca gataggccct cacttggctc
     3541 aagaaataca aggaacctgt gacaatccta actgtttgtt tctcttcttt tttatctgtc
     3601 aaattctgct ctagaaaatc atgccaatct tggtagctga gatttctaga cttagctgcc
     3661 tctttcagca cgctttcatc cgtcgcccga ctatccgcaa aatgtgttag agtaagctcg
     3721 gtatctggca tggctcccag caagtccaac atatcctcca aggctttggt tttgatacaa
     3781 gtgaagagga tttccttatg ataatccgca aaacgttctt gcaaggttac caacaaggcc
     3841 ttgatagcat ggggattatg ggctccatcc aaaatcatca agggatctct tgacacgatt
     3901 tccaaacgcc ctggccaact tgtttcttcc aaggcttgac caagaaagtc attgcttgct
     3961 agctctcgac catcttcttg acaaaaagta tcaagtaaag ctatggccat cccagcattc
     4021 tctatttggt acaaaccaag caggctagtc tggaagcgac cttgtctgac agcacttgta
     4081 tagtcaaaga cttcccctgt caccacactt tcttgatgac gaacctgata atctgtcccg
     4141 taggcaagtc tcggcgcatc tttcccttcc gcaatgcggt caatcacagt caaggcttct
     4201 ggagcaatac gccctgttac caagggcatc ccttgtttga taatacctgc cttctgctct
     4261 gctatggcct ccaaggtgtc accaagtagg gccacatgat ccaagccaat agttgtaatt
     4321 cctgtcaaaa tgggccgaca gacattggta ctatccaaaa gtccgcccat gccaacttcc
     4381 atgatggcca catctacttg ctctgaggca aagtagtcat aggccagggc tgtgataatc
     4441 tcaaactcgg ttgtgtcctg taaattggcg accgcttctc cctccagcaa agactgatag
     4501 tctgccatga gagcttctag cctcgcttct gagatcgatt ccccattgat gctaatctgg
     4561 tctgtgtaat gaatgagata gggcgaacta aacacgccaa ctctcagccc tagcttttct
     4621 agcatctttt tcaaaaaagc aatagtcgag cccttgccgt tagtccctcc aatatggaga
     4681 accttgagtt tgagatgggg attgccacgc aaagctaaca gttccaccat tcgttccaag
     4741 ccaaaatgcg gttgatccgt ccggtagtta gcaatccact gattgttttc aatttctttc
     4801 atcttattta tactgtttta aatctaaatt ttccgcttca tcagccagac gaatggcaga
     4861 ggcaatttca actgccatcc tgtgactagc tacgtcatgc acgcgcacca cttctacacc
     4921 ctgtctcgca gcgatactag ttacatgagc cgaagccgtg tcccgatttc ggaaaccaag
     4981 ctctgtctca ggattgactt caaaaccatt ctcctctaga atattgatga caaatcgctt
     5041 gcgcgacact ccgagaaaga ttggatagcc cttctgatgt agtttatcca ggtcccgtaa
     5101 aagaagcaga ttttctttct tggtcagacc aaagccaatt cctggatcca acaggatatt
     5161 ttctggtgca ataccagctt ctgccgctct cgctagtgct cgttcaaaga aagcctccat
     5221 caattcttca attggcaatg tttcaaagtc agctaactct ttttctgtaa aggtttgacc
     5281 aaagccaaaa tgagggaaga taagcgaact aggatgctga ggtcgcgcca taactggatt
     5341 aaacatgatg accactttcg ctccagcttt agcaaccaca tgggccattt tttcatcacc
     5401 catgagacca gtgatatcat taactagatt ggcacccgca gccaaagcag cctctgccac
     5461 ctgacttttc caagtatcga tagaaataag gacatcactt tccttgcgaa tcgctttgat
     5521 cactggaaca acacgctgga tttcctcttc tatctcaaca tagctactgc ccggccgagt
     5581 cgattctccg ccgatatcga gcatactggc tccttctgct atcaatttac gagcctgctg
     5641 gagtgcctgc tcaagagcaa aaaattgacc accgtccgaa aaggagtctg gggttacatt
     5701 gataattccg caaataactg tctttgcatg attggcttta cttgacatat cggtcactcc
     5761 ctcaaggatt ttcatcatat tatttttcta ttttaccata aaaagaaaaa gatggacacg
     5821 attacattca tctttctcta gtagaaagag gtcggttcct
//
LOCUS       DAVBCN010000099         5804 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVBCN010000099 DAVBCN010000000
VERSION     DAVBCN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.099"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            331..1377
                     /gene="pheS"
                     /locus_tag="VQ497_001882"
     CDS             331..1377
                     /gene="pheS"
                     /locus_tag="VQ497_001882"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET2518156.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1377..1886
                     /locus_tag="VQ497_001883"
     CDS             1377..1886
                     /locus_tag="VQ497_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2518157.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1963..4368
                     /locus_tag="VQ497_001884"
     CDS             1963..4368
                     /locus_tag="VQ497_001884"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET2518158.1"
                     /translation="MLVSYKWLKELVGIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEILSCEDVPETHLHVCQVNVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKKFADGIQILPENAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMRGVAHEVAAIYDKAVNFKEFTLTETNEAATDALSVGIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SHVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRTIAE
                     GAGLTEIITYVLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLEVTYTATAEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSVIEAALQPAAPFVEITKFPAVSRDVALLLKAAVTHQEVIDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEKKVNA
                     EVR"
     gene            complement(4541..5542)
                     /locus_tag="VQ497_001885"
     CDS             complement(4541..5542)
                     /locus_tag="VQ497_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET2518159.1"
                     /translation="MIKTFLSALSVILFVIPIITYSFFPSSNLNIWLSTRPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIADDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            5698..>5804
                     /locus_tag="VQ497_001886"
     CDS             5698..>5804
                     /locus_tag="VQ497_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2518160.1"
                     /translation="MQDNYTTKGKHLTIDSRRLIERWKKEGKSNREIASL"
BASE COUNT         1754 a         1083 c         1337 g         1630 t
ORIGIN      
        1 tcatcttatt tcaatccgct ataaagttag ataagaataa gctaacaagc agaagtaaga
       61 taggtcttgc aagaatggta aaaatctgat aaactaagta gtaattgaat aggaatctga
      121 aagactagta gcccaaggga agtatatcag ggaggagagc cgtgactgca agctctctat
      181 atgaaagctg ggtgaattca cttgcgcatg gatttagaaa tgaaggtctg acttgtcaga
      241 tgaaaacgga tggtaccgcg tgtcaacgct ccgagtggag tttttggcat gtggttttct
      301 ttttatctac gagagactga tggaggaaat atgtcaacta ttgaagaaca attaaaagcg
      361 cttcgcgaag aaacgctggc tagcttgaag cagattactg ctggaaatga aaaagagatg
      421 caagatttgc gtgtctctgt ccttggtaaa aagggttcgc tcactgaaat cctcaaaggg
      481 atgaaagatg tttctgctga gatgcgtcca atcatcggga aacacgtcaa tgaagctcgt
      541 gatgtcttga ctgctgcttt tgaagaaaca gctaagctct tggaagaaaa gaaagtcgcg
      601 gctcaactgg ctagcgagag tatcgatgtg acgcttccag gtcgtccagt tgcgactggt
      661 caccgtcacg ttttgacaca aaccagtgaa gaaatcgaag atatcttcat cggtatgggt
      721 tatcaagtcg tggatggttt tgaagtggag caagactact ataactttga acgtatgaac
      781 cttccaaaag accacccagc tcgtgatatg caggatactt tctatatcac tgaagaaatc
      841 ttgctccgta cccacacgtc tccagttcag gcgcgtgcta tggatgccca tgatttttct
      901 aaaggtcctt tgaagatgat ctcgccaggg cgtgtcttcc gtcgcgatac ggacgatgcg
      961 acccacagtc accaattcca ccaaatcgaa ggcttggtag ttgggaaaaa tatctctatg
     1021 gctgatcttc aaggaacgct tcagttgatt gtccaaaaaa tgtttggtga agagcgtcaa
     1081 attcgtttgc gtccatctta cttcccattc acagagccat ctgttgaggt ggatgtttct
     1141 tgcttcaagt gtggtggaga aggctgtaat gtatgtaaga aaacaggttg gatcgaaatt
     1201 atgggggccg gtatggttca cccacgtgtc cttgaaatga gtggtatcga tgcgactgta
     1261 tactctggct ttgcctttgg tcttggacaa gagcgtgtag ctatgctccg ttatggaatc
     1321 aacgatatcc gtggattcta ccaaggagat gtccgcttct cagaacagtt taaataatga
     1381 ttagaaaagt agaaatggca gatgttgagg tgttggctaa aattgccaaa caagcctttc
     1441 gtgaaacctt tgcgtatgat aatacggaag agcagttaca ggaatacttt gaagaggctt
     1501 atagtctgaa aactttgtca actgagttgg gaaatcctga ctctgaaacc tatttcatta
     1561 tgcatgagga ggagatagct ggttttctca aagtcaactg gggaagtgct caaactgaga
     1621 gagaattaga ggacgctttt gaaattcaac gcctctatgt gctacaaaaa ttccaaggat
     1681 ttggactagg taagcaactg tttgaattcg cacttgaact tgctacaaaa aatagttttt
     1741 cttgggcttg gctaggtgtt tgggagcata atacaaaagc tcaagcgttt tataaccgat
     1801 atggttttga aaaatttagc caacatcatt ttatggttgg tcaaaaagta gatacggatt
     1861 ggttactgag aaagaaatta aggtaagaag agaattcttc tattgaaatg ataggaaagg
     1921 aaaagaacca gtgtggcaat ggtcattctg gagaactaaa ttatgcttgt atcttataaa
     1981 tggttaaaag aattggtggg cattgatgtg ccatcacagg agttggctga aaaaatgtca
     2041 accacaggga tcgaggtcga aggtgtcgaa tcaccagctg ccggtctttc aaaaattgtc
     2101 gtcggtgaga tcttgtcttg cgaagatgtg ccagaaactc accttcatgt ttgtcaggtt
     2161 aacgttggcg aagaagaaga gcgtcagatc gtttgtggtg ccccaaatgt gcgtgcaggc
     2221 atcaaggtta tggtggctct tccgggagct cgtatcgctg acaattacaa aatcaaaaaa
     2281 ggaaaaatcc gtggcttaga gtcacttgga atgatctgtt cacttggtga attgggtatt
     2341 tctgactcag ttgtacctaa gaaattcgca gatggtattc aaattttgcc agaaaatgcc
     2401 gttccaggtg aggaagtctt ttcatatcta gacttggatg atgaaatcat cgaactttct
     2461 atcacaccaa accgtgcaga tgccctttct atgcgtggag tggctcacga agtggcagcc
     2521 atatatgaca aggcagtcaa ctttaaagaa tttactttga cagaaacaaa tgaagctgcg
     2581 acagatgccc tttctgtggg cattgagaca gacaaggcgc cttactatgc agctcgtatc
     2641 ttggacaatg tgactatcgc accaagtcca caatggttgc aaaaccttct catgaacgaa
     2701 ggcatccgtc ccatcaataa cgttgtagac gtgacaaact acatcctgct ctactttggt
     2761 caacctatgc atgcttttga cttggacaca tttgaaggga ctgacatccg tgtgcgtgaa
     2821 gcgcgtgctg gtgaaaaatt ggtgaccttg gacggtgaag aacgtgactt ggacgtgaat
     2881 gacctagtca tcactgttgc agacaagcca gtagcccttg caggtgtcat gggtggtcaa
     2941 gcaacagaaa tctctgaaaa atctagtcat gttgtccttg aagctgctgt tttcaatggc
     3001 aaatctatcc gtaagacaag tggtcgcctg aaccttcgtt ctgagtcatc ttctcgcttt
     3061 gaaaaaggaa ttaatgtggc aacagttaat gaagcccttg atgcggcagc tagcctgatt
     3121 gcggaacttg caggtgcgac ggtgcgtaag ggtatcgttt cagcgggtga gcttgatact
     3181 tcagatgtag aagtttcttc aactcttgct gatgttaacc gtgtcctcgg aactgagctg
     3241 tcttatgctg atgtcgaaga cgtcttccgt cgtcttggct ttggtctttc tggaaatgca
     3301 gacagcttta cagtcagcgt accacgtcgt cgttgggata tcacaatcga agctgatctc
     3361 tttgaagaaa tcgctcgtat ctatggatat gaccgcttgc caaccagcct tccaaaagac
     3421 gatggtacag ctggtgaatt gactgtgaca caaaaactcc gccgtcaagt tcgtaccatt
     3481 gctgaaggag caggtttgac agaaatcatc acctatgttc taacaactcc tgaaaaagca
     3541 gttgagttta cggctcaacc aagtaacctt acggaactca tgtggccaat gactgtggat
     3601 cgttcagtcc tccgtcaaaa tatgatttca ggtatccttg ataccgttgc ctacaacgtg
     3661 gctcgtaaga ataaaaactt ggccctttac gagattggaa aagtctttga acaaacaggt
     3721 aatccaaaag aagaacttcc aaatgaaatc aacagttttg cctttgcctt gacaggcttg
     3781 gttgctgaaa aagatttcca aacagcagca gttccagttg atttcttcta tgctaaagga
     3841 atcctagaag cgctctttac tcgcttggga ctagaagtga cctatacagc aacagctgag
     3901 attgccagcc ttcacccagg acgtacagca gtcatttcac tcggtgacca agttcttggt
     3961 tttcttggcc aagtgcatcc agtcactgct aaggcttacg atattccaga aacgtatgtg
     4021 gctgagctca acctttcagt catcgaagct gcccttcaac ctgctgctcc atttgtggaa
     4081 atcacgaaat tcccagcagt cagccgtgac gttgcccttc tcctcaaggc agcagtaact
     4141 caccaagaag ttatagatgc tatccaagct gccggcgtga aacgtttgac agatatcaaa
     4201 ctctttgacg ttttctcagg tgagaaattg ggacttggta tgaagtcaat ggcttatagc
     4261 ttgaccttcc aaaatccaga agacagctta acggacgaag aagtcgcacg ctacatggaa
     4321 aaaatccaag catcgcttga aaaaaaagtg aacgcagaag tgcgttaaac gatagtagga
     4381 tacaaattcc ccctctagtt tatgaacact agaggggtga ttttatataa tactcaatga
     4441 aaatcaaaaa gcaaactagg aagctagccg taagctgtac ttgagtactg taaggcgagg
     4501 ctgacgtggt ttgaagagat tgtcgaagag tataaaataa ttaaaatgtg atttctgtaa
     4561 aaatacatct atgatcagag ttttgaaaac ttacaatgtc taaatcttta acatagtagt
     4621 ggtttttagg caataaaata tgatctattg ttgcattaaa aagttttgga ctttggctat
     4681 tccaagttcc tctttcaaaa ggtggtagta catttaatgc gtccctatga gagcttattt
     4741 ttgcaagtgc tccatgacgc atagttgcat taaaatcatc tgcaataata gcctttggat
     4801 attttgaagc caattgatta tgaatgatgt ttaagtcttg cttccagatt tccattaaac
     4861 ctggcagagg aggagcagta tgcaaggcaa tgatatctgg tatattttgt tttctcgaat
     4921 gtaatacaat tgtcccgaac cgtgttgtat gaaaagtttt agcttctgta tagaaaccat
     4981 aacttttctt gacaatcaca gtcacaggag ctattccact attggtaggt ggagaagtga
     5041 aaatatcata gttggccata gaaagtccaa cttgatgaaa caatagtttg attctctggt
     5101 tttcttgctc acctctgata ttggtagcta gttcagggaa tatagccata tcggcgtcaa
     5161 aatggctaaa aattcgctca atatgttgtg cttctatttg attagcgacg ttccaagtta
     5221 ctaattttaa ggttttagtc ttatttgatg cagaagaaag ggttttatct gttccgaata
     5281 atagtaatat gagcgatagt aagagcaaaa tgccagagta aaaccgtatt tgtttatttt
     5341 tcttgtaaaa agatagggaa aaaaatacga aacttaaaat agcagccata gttgcagtag
     5401 ctaaggggaa ggcataaatc tgtgccaaga taggtctggt agatagccaa atattaagat
     5461 tagaagatgg aaaaaaagaa taagttatga tagggataac aaagagaatg accgaaaggg
     5521 cagagagaaa tgttttaatc attgtgtgag tacctcaatt tgcaatggtt gctcggagct
     5581 aatgtgactt tgtgacttta cttgattgat attctttttc ttgtcttcct catagattaa
     5641 gtacaaccca agtaaaaaaa gtccaattta agattgaaat gattaaaagg cacagaaatg
     5701 caagacaatt atactacaaa aggcaaacat ttgacaatcg atagccgtcg cttaatcgaa
     5761 agatggaaaa aagaagggaa atcaaataga gaaattgcct ctct
//
LOCUS       DAVBCN010000100         4988 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVBCN010000100 DAVBCN010000000
VERSION     DAVBCN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.100"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(44..283)
                     /locus_tag="VQ497_001887"
     CDS             complement(44..283)
                     /locus_tag="VQ497_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET2518161.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(303..575)
                     /gene="rpsP"
                     /locus_tag="VQ497_001888"
     CDS             complement(303..575)
                     /gene="rpsP"
                     /locus_tag="VQ497_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET2518162.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            745..954
                     /locus_tag="VQ497_001889"
     CDS             745..954
                     /locus_tag="VQ497_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518163.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            1433..1654
                     /locus_tag="VQ497_001890"
     CDS             1433..1654
                     /locus_tag="VQ497_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518164.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDFQDDDEIPTII"
     gene            1737..2540
                     /locus_tag="VQ497_001891"
     CDS             1737..2540
                     /locus_tag="VQ497_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET2518165.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQSSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(2596..4137)
                     /locus_tag="VQ497_001892"
     CDS             complement(2596..4137)
                     /locus_tag="VQ497_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2518166.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMEMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DLPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(4139..4621)
                     /locus_tag="VQ497_001893"
     CDS             complement(4139..4621)
                     /locus_tag="VQ497_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET2518167.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVYFCFTPQEQSTVGVGTPDIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            complement(4621..>4988)
                     /locus_tag="VQ497_001894"
     CDS             complement(4621..>4988)
                     /locus_tag="VQ497_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S rRNA methyltransferase"
                     /protein_id="HET2518168.1"
                     /translation="KLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAELLKNI
                     KKSSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGTDIDAACGQ
                     LLFNTMKRDRQKAVAAVNP"
BASE COUNT         1529 a         1058 c          935 g         1466 t
ORIGIN      
        1 cttctattcc acacaaaaaa gcgagacatc cgtcccgccc ttcttatttt tcgtcaataa
       61 cgattcttac ttttttgtat tcagttggga cagagtagac aatcgttctt atcgcagaaa
      121 tagtgcgacc cttacgaccg attacacgac ccacatcgct ttgatcaaga ttcaaatgat
      181 attccaaaaa ttctggtgta tcctcaatct tgatagttaa ggcatctggt tgtgaaatta
      241 agggtttcac aatcgcaata atgagatttt caatcgtatc catctgtcaa cctactttaa
      301 acttattttg aaaatttaga atcgtggaat tttttcaata cgccttcttt tgaaaggatg
      361 ttacgtactg tgtctgaagg ttgagctcca ttagccaacc atgcaagaac gcggtcttct
      421 ttcaaagtta cttggttttc agcaacaagt gggttgtaag ttccaactgt ttcgatgaaa
      481 cgtccgtcac gtggtgaacg tgaatctgct acgttgatac ggtagaaagg ttttttctta
      541 gaacccatac gagtcaaacg gattttaact gccattttta aagtctcatt tctttaattt
      601 tttatttcgg tgaaatagct gagctattta gcacatgttc tattatagca gatttctggc
      661 atgtgtcaag aaaaaaactt gacagactat aaatttctta aagtatttag aaatttgtta
      721 gaataggaaa taaatgtttg aaagatgcta tccgtgaata ctattttaga aaaattttat
      781 aaagaacacc aagtcaaacc cttcatatcg cctgaacgag agttggatac ttggcttcta
      841 agtcccaagc ccgttcccaa gcgaaatatg gacttattag tagatgattc actagcagga
      901 gatatcattt tactatggag aatccagttt ggtactttta ccactgaaac atgattttaa
      961 aaactcccat tcgaatatga ttcaaaattc gcttagaatc aatgttttcc cgctttttca
     1021 aaaaataaat tttcatctaa tttaaataac tttcaattaa gtgtgaattt tgcttaaaat
     1081 cattaaaaag caccccatgg tgtgaaccat gaagtgttgt aaatgctgtg ttgtagctag
     1141 ttcttagcgt ttagagaact ggctagcttt acgagctttc ttaagatctg gtttccaaaa
     1201 tactttgagt acacttatgg tattgatgcc cctaaacatc taaaaactct ggtagaaaaa
     1261 gggtatgctc ttgtcgaaac ttcttttgac tcacttgacc atctaaataa taccatgaaa
     1321 aagaatatcc tgaagaagaa gggagttact ggactttcta aaatgaaatc ttgcgacctg
     1381 gatcaagccc ttcatgagca tttttcagaa gaagaattag ctggtcactt tcatgtcctt
     1441 ctatggactt ttttacaatg gcattgctat cacacccaat acctatctaa gcgcctggtt
     1501 cgtaaacttt attgcagctc ttcctctaaa tttcctaatt gttgaaccaa ttgcccgttt
     1561 tatactaagt tcttttcaga aaccatttac tggggaagaa gttgaagaag ttgaagattt
     1621 tcaagatgat gatgaaatcc caactattat ctaagccagt tctgtaaact actaatattt
     1681 gaaatccact tctttttagg gtacaatggt ataaatgaat ttttgagagg atcagaatga
     1741 aaaaactagc aacccttctt ttactgtcta ctgtagccct agctgggtgt agcagcgtcc
     1801 aacgcagtct gcgtggtgat gattatgttg attccagtct tgctgctgaa gaaagttcca
     1861 aagtagctgc ccaatctgcc aaggagttaa acgatgcttt aacaaacgaa aacgccaatt
     1921 tcccacaact atctaaggaa gttgctgaag atgaagccga agtgattctc cacacaagcc
     1981 aaggtgatat tcgcattaaa ctcttcccta aactcgctcc tctagcggtt gaaaatttcc
     2041 tcactcacgc caaagaaggc tactataacg gtattacctt ccaccgtgtc atcgatggct
     2101 ttatggtcca aactggagat ccaaaagggg acggtacagg tggtcagtcc atctggcatg
     2161 acaaggataa gactaaagac aaaggaactg gtttcaagaa cgagattact ccttatttgt
     2221 ataacatccg tggtgctctt gctatggcta atactggtca accaaacacc aatggcagcc
     2281 agttcttcat caaccaaagc tctacagata cctcttctaa actccctaca agcaagtatc
     2341 cacagaaaat tatcgaagcc tacaaagaag gtggaaaccc tagtctagat ggcaaacacc
     2401 cagtctttgg tcaagtgatt gacggtatgg atgttgtgga taagattgct aaggccgaaa
     2461 aagatgaaaa agacaagcca actactgcta tcacaatcga cagcatcgaa gtggtgaaag
     2521 actacgattt taaatcttaa aaaccaaaaa aatacagtat ccacattcgg tactgtattt
     2581 cttttactct cattcttaag ttaaattatt aaaatcccat atttggtcta tccagccttc
     2641 ataaaagtct ggctcgtggc agaccataag gatagatccc ctatattctt tgagagcgcg
     2701 tttgagctca tcctttgcat ccacatccaa atggttggtc ggctcgtcca gcactaaaac
     2761 gttgttttca cgattcatca agagacagaa acgaaccttg gcttgctctc cccctgataa
     2821 tacttgaatc tggctttcaa tatgtttggt tgtcaaacca caacgggcaa gggctgcacg
     2881 gacttctgct tgattaaggg caggaaaggc attccagaca gcttcaagag gagtttggcg
     2941 attaccgcct tctacttcct gctcaaaata accaagttct aaataatctc cacgctccac
     3001 ttccccagcg attggcgaga taatgcccaa gagactcttc aagagagttg tttttccaat
     3061 accattagca ccaataatcg caaccttttg attgcgttcg aaggtaagat ttaaaggctt
     3121 agtaagagga aggtcgtaac caatttgcaa gttcttggct tggaagataa agcgccctgg
     3181 tgtacgagct ggtttgaaat caaaggatgg ttttggtttc tcactttgga gttcgataat
     3241 atccatctta tccaatttct tttgacgaga catagccata ttacgagttg caacacgggc
     3301 tttattacga gccacaaagt ccttgaggtc tgcaatctct ttctgctggc gttcgtaggc
     3361 tgcctctagc tgagatttct tcatagcata aacttcttgg aactggtagt agtcaccaga
     3421 gtaacgcgtc agctgttgat tttccacatg atagacaata ttgataacgt cattgaggaa
     3481 tggaatatcg tgcgaaatga gaacaaaggc attctcatag ttttggagat agcgcttgag
     3541 ccaatcaata tgctcagcat ccaagtagtt ggtcggctcg tccaacagca agatatcagg
     3601 cttttcaagg agaagttttg ccaaaagcac cttggttctt tgcccacctg acaaagaagt
     3661 tacatccgta tccatgccaa agtccataac accaagagca cgcgctactt cgtcaatctt
     3721 agcatccaag gtatagaaat cacgactctc cagacggtct tgaagttctc ctacttcttc
     3781 catgagagca tcaacatccg caccgtcttc agccatttcc atatagaggt cattgatacg
     3841 agcttcagct ttgaaaagct catcaaaagc cgtacggaga acatcacgca ccgactgtct
     3901 ttcagcaagg acagagtgct gatccaagta accagccgtc acatatttgg accactcaac
     3961 ctttccttca tctggcagca ttttaccagt cacgatactc ataaaggttg attttccttc
     4021 accattggca ccgaccaggc cgatatgttc tcccttgagg agacggaagg acacatcttc
     4081 aaaaattgca cggtcaccaa aaccgtgact cagattttta acttctaaaa tactcatttt
     4141 aattccttat cttgttttta tgtaatcgtt tataaaggag ccaagccaga tagccaccca
     4201 aagtgttggt ccacaaatca tcaatctcaa agacgcgatt gaaatcaaag aaaaagtcca
     4261 agattaattg cgtacactcg attccaagac tcacaagaaa actaaaaaga aggacctttt
     4321 ttgttttccg caaatttgga aatagataaa ggagttggaa aatcagagga aaaaacaaga
     4381 agacattgag gatattttgt aaaaaaatcc aacataattg tccaatgtca ctcacttcgc
     4441 ccagtttcca gagagaattg aaaggagtca aaagaaaaac caggcgtcca agatgctgaa
     4501 tatctggagt tcccactccc acggtagatt gttcttgagg agtaaagcaa aaatagacaa
     4561 tgcaaatgct atagaaaatg actccccaga ccaaaatatg attataagtc ttcttcatca
     4621 ttaaggattt accgctgcga ctgccttctg gcggtcacgt ttcattgtgt taaagagcaa
     4681 ttgtccacaa gctgcgtcaa tatctgtacc atgctcttga cgaaccacac agttgccccc
     4741 ttttttctta agcgtatcat agaaagccaa cacgcgctct ttgggactac ggctatattg
     4801 gtcatgctca ctaactgggt tataaggaat caagtttaca taagacgatt tcttgatgtt
     4861 cttgagcaat tcagccaatt ccaaggcttg ttccacaccg tcgttgactt cattaagcat
     4921 gatatattca aaggttacac gacggtttgt tgtctcaatg tagtattcaa tagcagcaaa
     4981 gagttttt
//
LOCUS       DAVBCN010000101         4922 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVBCN010000101 DAVBCN010000000
VERSION     DAVBCN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.101"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            242..460
                     /locus_tag="VQ497_001895"
     CDS             242..460
                     /locus_tag="VQ497_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HET2518169.1"
                     /translation="MKKISVTVIGYFEINIDENITDILYVNGTAILYHYLRSIVSIVS
                     AIDSSEAMLLPTINVLELLDKSQPFEEE"
     gene            1505..2497
                     /locus_tag="VQ497_001896"
     CDS             1505..2497
                     /locus_tag="VQ497_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2518170.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYDGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            2499..3317
                     /locus_tag="VQ497_001897"
     CDS             2499..3317
                     /locus_tag="VQ497_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET2518171.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVIYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            3319..4104
                     /locus_tag="VQ497_001898"
     CDS             3319..4104
                     /locus_tag="VQ497_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2518172.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            4143..4421
                     /locus_tag="VQ497_001899"
     CDS             4143..4421
                     /locus_tag="VQ497_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HET2518173.1"
                     /translation="MFVIEEVKDENQKKAVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTRFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            4406..4603
                     /locus_tag="VQ497_001900"
     CDS             4406..4603
                     /locus_tag="VQ497_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2518174.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFPQLWNPQNPCQILIKKLE"
BASE COUNT         1415 a          810 c         1030 g         1667 t
ORIGIN      
        1 gactacgtca gttccatcta caacctcaaa acagtgtttt gagcaacctg aggctagttt
       61 cctagtttgc tctttggttt tcatttagta taacagattg ttagaagttg gtttaaattt
      121 cctaatcagt ttgttcacat ttaccttcga tatattatat cccatagtta aggttggtca
      181 tacagatgat tatagtcatg gagccgtaaa acttagtgtt tctttagttg acaaagatgc
      241 catgaaaaaa atatctgtaa ctgtaatagg atattttgaa ataaatatag atgaaaatat
      301 caccgatatt ctatacgtaa atggtactgc tattctttat cattatttac gttctattgt
      361 ttcaatagtt tcggcaattg atagcagtga agcaatgttg ctacctacca ttaatgtttt
      421 agagttacta gataaatctc aaccttttga agaagaataa tttattagct cactaaattg
      481 agggtaagga aaagtaaaat cagtaagaaa aatgtcttgc attatacagc aaccttttgg
      541 gaatgagtag atggattgaa taaaatttta ttaagagtgg atgatttatc tgtagattat
      601 tattggacag ttagtcttga agtagtctaa gaattaggtt ataatcagta gaatccttgc
      661 taataatgag gaggttagtt tatgtatagt agactgaatc taaaatagta cgaaacaatt
      721 gctaaaacat ttatagaaat taattttact ttcccaatcg atttgttctc atcttatttc
      781 aatccgctat atattatggt atcgaatctt catcagaatg ataaaattaa tcaattgata
      841 tctgattaca aacagaatat gaaagctttt tatatcacta ttgaaaaatt tatacgagat
      901 gatgaaagcc ttaagtgtta ttttataaag gttatttcaa gtcgttccaa ggtaacaagt
      961 ctagatcaga ttgaagctga taaaacgata caaagaaaat attcaagtga gctaaaaaaa
     1021 tttattggat tttataatga gattatttgt gaggaaaata gtttcctaca tgtacgaaag
     1081 aggtggtcga gttggtttag gtagtcgatg cgtgagttga taattctcag ggtatggact
     1141 tctttttcat gaatgaggta aaagagcagg tattgtttag agacaatcat tctgagcata
     1201 ttttctggat agagggagta tccgatttta tgatcaaagt taataccgcc ctctggtgag
     1261 aagatgagta ggttggtaat ttaaactatt aaacagaatt tttgattaaa agtattattt
     1321 tatgagagaa atcctaattt cacaatccat aggcaaactc ttgcatttcg ttttttattg
     1381 gactataata ggttggtata aagccttttg tagtaataaa atgtagaagg tgtagaaagc
     1441 aaggatttag aatatttgta gttaaaaaca caatgttgct attccttacg atagggagat
     1501 agatatggca atgatagaag tggaacatct tcagaaaaat tttgtgaaga ctgttaagga
     1561 accgggcttg aagggggctt tgcgctcctt tattcatcct gaaaagcaga cctttgaagc
     1621 ggtcaaggat ttgacctttg aggttccaaa agggcagatt ttaggattta tcggggcaaa
     1681 tggtgctggg aagtcgacaa ccattaaaat gctgacagga attttgaaac caacatctgg
     1741 tttttgtcgg attaacggca agattcccca ggacaatcgg caagattatg tcaaagatat
     1801 tggcgtagtc tttggacaac gcacccagct atggtgggat ttggctctgc aagagaccta
     1861 cactgtctta aaagagattt atgatgtgcc agactcgctc tttcataagc gtatggactt
     1921 tttgaatgaa gtcttggatt tgaaggactt tatcaaggat cccgtgcgga ctctttcact
     1981 gggacaacgg atgcgggcgg atattgcggc ctccttgctc cacaatccca aggttctttt
     2041 tttagatgag ccgaccattg gtttggacgt ttcggttaag gataatattc gtcgggcaat
     2101 tactcagatc aatcaagagg aagaaactac cattcttttg accactcacg atttgagtga
     2161 tattgagcaa ctttgtgatc ggattttcat gattgacaag gggcaagaga tttttgatgg
     2221 aacggtgagc caactcaagg agacctttgg taagatgaag actctctctt ttgaactgct
     2281 accaggtcaa agtcatctcg tctctcacta tgacggtctg tctgatatga ccattgatag
     2341 acaaggaaac agcctcaaca ttgaatttga tagttctcgc taccagtcag ctgacattat
     2401 caagcaaacc ctgtctgatt ttgaaatccg cgatttgaag atggtggata cggatattga
     2461 ggatattatc cgtcgcttct accgaaagga gctctaggat gatcaaattg tggagacgtt
     2521 ataaaccctt tatcaatgca ggggttcagg agttgattac ttaccgagtc aactttattc
     2581 tctatcggat tggcgatgtc atgggggctt ttgtggcctt ttatctctgg aaggctgtct
     2641 ttgattcttc gcaagagtct ttgattcagg gcttcagtat ggcggatatc accctctaca
     2701 tcatcatgag ttttgtgacc aatcttctga ctagatccga ttcgtccttt atgattgggg
     2761 aggaggtcaa ggatggctcc attatcatgc gtttgttgcg accagtgcat tttgcggcct
     2821 cctatctttt caccgagctt ggttccaagt ggttgatttt tatcagcgtt ggccttccat
     2881 ttttaagtgt cattgtcttg atgaaaatca tatcgggtca aggtattgta gaggtgctag
     2941 gattaactgt catttatctt tttagcttaa cgctcgccta tctgattaac tttttcttta
     3001 atatttgctt tggattttca gcctttgtgt ttaaaaatct ttggggttcc aacctactta
     3061 agacttccat agtggctttt atgtcgggga gtttgattcc cttggcattc tttccaaagg
     3121 ttgtttcaga tattctctcc tttttgcctt tttcatcctt gatttatact ccagttatga
     3181 tcattgttgg aaaatacgat gccagtcaga ttcttcaggc actccttttg cagttcttct
     3241 ggctcttagt gatggtggga ttgtctcagt tgatttggaa acgggtccag tcctttatca
     3301 ccattcaagg aggttagtat gaaaaaatat caacgaatgc atctgatttt tatcagacaa
     3361 tacatcaaac aaatcatgga atataaggtg gattttgtgg ttggtgtctt gggagtcttt
     3421 ctggctcaag gcttgaatct cttgtttctc aatgtcatct ttcaacatat tccattccta
     3481 gaaggctgga cctttcaaga gatagctttc atttatggat tttccttgat tcccaaggga
     3541 atggaccatc tcttttttga caatctctgg gcactagggc aacgcctagt ccgaaaaggg
     3601 gagtttgaca agtatctgac tcgtcccatc aatcctctct ttcacatcct agttgaaacc
     3661 tttcagattg atgccttggg tgaactctta gtcggtggta ttttattggg aacaacagtg
     3721 accagcattg tttggactct tccgaaattc ctgcttttcc tagtttgtat tccttttgcg
     3781 accttgattt atacttctct taaaatcgca acagccagta tcgccttttg gactaagcag
     3841 tcaggcgcca tgatttacat cttctatatg ttcaatgact ttgctaagta tccgatttct
     3901 atttacaatt ctcttcttcg ttggttgatt agctttatcg tgcctttcgc ctttacagcc
     3961 tactatccag ctagctattt cttacaggaa aaggatgtgt tctttaacgt aggaggtttg
     4021 atgttgattt ctctggtttt ctttgttatt tcccttaaac tttgggataa gggcttagat
     4081 tcctacgaaa gtgcgggttc gtaaaagcta aagtaagact aaaatcaaga aagaaactta
     4141 tgatgtttgt aattgaagaa gtcaaggatg aaaatcaaaa aaaggcagtt gtagctgagg
     4201 ttttgaagga tttgccagaa tggtttggaa tcccagaaag cacacaagcc tatatagaag
     4261 gaaccacgac actgcaagtt tggaccgcct atcaggagag tgatttgact agatttgtaa
     4321 gcttatccta ttcgagtgaa gattgtgcag agattgattg tctcggcgta aaaaagctta
     4381 tcaaggtaga aaaattggga gccaattgct tgctacttta gagagtgaag ctcgtaaaaa
     4441 agttggttat ctgcaggtca aaacagtggc agaaggttct aataaagatt atgatcgaac
     4501 aaatgacttt tatcgaggtc ttggctttaa aaagttagag atttttcctc aactatggaa
     4561 tccgcaaaat ccttgtcaga ttttgattaa aaagcttgaa taatattact tgacatctat
     4621 tctcagagtg ccatactgta agtgtaatcg ccgatttagc tcagttggta gagcaaggca
     4681 ctcgtaaagc ctaggttata ggtagataaa cgactgagga gttgaaaaaa tagataggta
     4741 gaagataacc gttaagcctt attcttagcg gttatttata ttgtttaata gcgctgatat
     4801 ttcatcaatt atgcctgttt tcgtgtttct ggtagttgtt caagtttatt gctactattt
     4861 ttgatggtat gaatgtgctt ataatgtatc ccggttaacg aaagttttgg acttatactc
     4921 tt
//
LOCUS       DAVBCN010000102         4890 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVBCN010000102 DAVBCN010000000
VERSION     DAVBCN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.102"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            21..866
                     /locus_tag="VQ497_001901"
     CDS             21..866
                     /locus_tag="VQ497_001901"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET2518175.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            complement(1013..2074)
                     /gene="dinB"
                     /locus_tag="VQ497_001902"
     CDS             complement(1013..2074)
                     /gene="dinB"
                     /locus_tag="VQ497_001902"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET2518176.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSKKERGVRLLGITMTGF"
     gene            2422..4746
                     /gene="pflB"
                     /locus_tag="VQ497_001903"
     CDS             2422..4746
                     /gene="pflB"
                     /locus_tag="VQ497_001903"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HET2518177.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKSNFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
BASE COUNT         1421 a         1003 c          974 g         1492 t
ORIGIN      
        1 tataatatag ggagtaatct atgtatctta ttgaaatttt aaaatctatc ttcttcggga
       61 ttgttgaagg aattacggaa tggttgccga tttccagtac aggtcacttg attttagcag
      121 aggaattcat ccaataccaa aatcaaaatg aagcctttat gtccatgttt aatgtcgtga
      181 ttcagcttgg tgctatttta gcagttatgg tgatttattt taacaagctc aatcctttta
      241 aaccgactaa ggacaaacag gaagttcgta agacttggag actatggttg aaggtcttga
      301 ttgctacttt acctttactt ggtgtcttta aatttgatga ttggtttgat acccacttcc
      361 ataacatggt ttcagttgct ctcatgttga ttatctacgg ggttgccttc atctatttgg
      421 aaaagcgcaa taaagcgcgt gctatcgagc caagtgtaac agagttggac aagcttcctt
      481 atacgaccgc tttctatatc ggactcttcc aagttcttgc tcttttacca gggactagcc
      541 gttcaggtgc aacgattgtc ggtggtttgt taaatggaac cagtcgttca gttgtgacag
      601 aatttacctt ctatcttggg attcctgtta tgtttggagc tagtgcctta aagattttca
      661 aatttgtgaa agccggagaa ctcttgagct ttgggcaatt gtttttgctc ttggtcgcga
      721 tgggagtagc ttttgcggtc agcatggtgg ctattcgctt cttgaccagc tatgtgaaaa
      781 aacacgactt cacccttttt ggtaaatacc gtatcgtgct tggtagtgtt ttgctacttt
      841 acagttttgt ccgtttattt gtataagaaa aaccttgaag gggcaactct tcaaggtttt
      901 atactcttcg aaaatctctt caaaccgcgt cagctttatc tgcaacctca aaacagtgtt
      961 ttgagcagcc tgcggctagc ctcctagttt gctctttgat tttcattgag ctttaaaatc
     1021 cagtcatggt aatccccaat aggcggacac ctctttcttt cttgcttaat tcttcataga
     1081 gttgcagggc tatttggctt atctgactag catcttgtgt tttttgagca agactttttc
     1141 gtttggtaag agttgaaaag tcctcgtagc ggattttcaa aatgacaatt tttccagctt
     1201 tttcttgttg atgtagattg agagcgactt tttctgatag aagagtcagc tcttttttga
     1261 tatcttcctc agcaaggaga atcttcccgt aggttttctc cttgccgatt gatttacgga
     1321 tgcgattgga tttgactgga gagttgtgaa tgccacgagc ctttcgatac agatcatagc
     1381 ctagtctacc aaaacggtct attagggtta cctcaggaac ttcaagtaaa tcagcaccag
     1441 taaaaacgcc catttgatga agacgttcta ctgtcttttt tcctactcca tgaaatttgg
     1501 aaatatccat ttgtttgaga aaatcctcag cctgttcagg tagaatcact gtcaaaccat
     1561 gtggtttttg ataatcactc gccattttag ctaagaattt gttgtaagaa acgcctgcgg
     1621 aagcagttag atggagttct tgccagatat ctttttgaat gaggcgagca attttgaccg
     1681 ctgacttgat accgagttta ttttctgtca catccaaata ggcttcgtca atgctcatgg
     1741 gttcaatcaa atctgtatag cgcttaaaaa tagctcgaat ctggagtccc acagacttgt
     1801 atttctcata attccctgag ataaagacag cctggggaca acgttcataa gcttccttgg
     1861 aactcatggc agaatggaca ccaaaagctc ttgcctcata actacaggta gaaacgactc
     1921 cccgtccacc tgtttgccga gggtcgcttc caataatgac aggttttcct ctgagtttag
     1981 gattatccct gatttccact gcagcaaaaa aggcatccat gtcaatatgg atgatttttc
     2041 ttgacaaatc atttaacaaa ggaaaaatca acatgcctag caccttttta tactcttcga
     2101 aaatctcttc aaaccccgtc agctctatct gcaacctcaa aacagtgttt tgagcaatct
     2161 gcggctagct tcctagtttg cttttcgatt tccattgagt gttactgctt attttctttt
     2221 attataccct tttttctgaa aaaaagaaaa aaggacttta ttttttcaaa aatataatac
     2281 agtttgaaat aaaatataga ctgttttaga aaagaaagtg taaaaatagg gaattttcac
     2341 ttgttgaaat cggttactat atggtatact tgtcttatga atgtaacaga tgactgttac
     2401 tagaaaaaag aggacattaa tatggttgtt aagacagttg ttgaagcaca agatattttt
     2461 gacaaagctt gggaaggctt caaaggcgta gattggaaag aaaaagcaag tgtatcacga
     2521 tttgtacaag ctaactacac accttatgat ggagacgaaa gcttccttgc aggaccaaca
     2581 gagcgttcac ttcacatcaa gaaaattgta gaagaaacta aagcacacta cgaagaaact
     2641 cgtttcccaa tggacactcg tccaacatct atcgctgata tccctgctgg atttatcgac
     2701 aaagaaaatg aagttatctt cggtatccaa aacgatgaac tcttcaaatt gaacttcatg
     2761 ccaaaaggtg gtatccgtat ggctgaaact actttgaaag aaaatggata cgaaccagac
     2821 ccagctgttc acgaaatctt cactaaatat gtaacaacag ttaacgacgg tattttccgt
     2881 gcctacactt caaatattcg tcgcgctcgt cacgcacaca ctgtaactgg tcttccagat
     2941 gcatactcac gcggacgtat catcggtgtt tacgcacgtc ttgctcttta cggtgcagac
     3001 tacttgatgc aagaaaaagt aaatgactgg aatgcaatca aagaaatcga tgaagaaaca
     3061 atccgtcttc gtgaagaagt aaaccttcaa taccaagcat tgcaacaagt tgttcgcctg
     3121 ggtgaccttt acggggttga tgttcgcaaa ccagcgatga acgtgaaaga agcaatccaa
     3181 tgggttaaca ttgctttcat ggctgtctgc cgtgtgatta acggtgctgc tacatctcta
     3241 ggtcgtgtac caatcgtatt ggacatcttt gcagaacgtg accttgctcg tggtacattt
     3301 actgaatcag aaatccaaga attcgttgat gatttcgtta tgaaacttcg tacagttaaa
     3361 tttgctcgta caaaagctta tgaccaattg tactcaggtg acccaacctt tatcacaact
     3421 tctatggctg gtatgggtaa cgacggtcgt caccgtgtta ctaagatgga ctaccgtttc
     3481 ttgaacactc ttgacaacat cggtaactca ccagaaccaa acttgacagt tctttggact
     3541 gacaaattgc catacaactt ccgtcgctac tgtatgcaca tgagccacaa acactcttct
     3601 atccaatacg aaggtgtaac aacaatggct aaagacggat atggtgaaat gagctgtatc
     3661 tcatgctgtg tgtctccact tgatccagaa aatgaagaac aacgccacaa catccagtac
     3721 ttcggtgctc gtgtaaacgt tcttaaagcc cttcttactg gtttgaatgg tggttacgac
     3781 gatgttcaca aagactacaa agtatttgat atcgaaccaa tccgtgacga agttcttgaa
     3841 tttgaatcag ttaaatcgaa ctttgaaaaa tctcttgact ggttgactga cacttacgta
     3901 gatgccttga acatcatcca ctacatgact gataggtaca actacgaagc tgttcaaatg
     3961 gccttcttgc caactaaaca acgtgccaac atgggattcg gtatctgtgg atttgctaac
     4021 actgttgata cattgtcagc tatcaaatac gctacagtta aaccaatccg tgacgaagat
     4081 ggctacatct acgattacga aacaatcggt gactacccac gctggggtga agatgaccca
     4141 cgttcaaacg aattggcaga atggttgatc gaagcttaca caactcgtct acgtagccac
     4201 aaactataca aagacgcaga agctacagta tcacttttga caatcacatc taacgttgct
     4261 tactctaaac aaactggtaa ctcaccagtt cacaaaggtg tatacctcaa cgaagatggt
     4321 tctgtgaact tgtctaaact tgaattcttc tcaccaggtg ctaacccatc taacaaagct
     4381 aaaggtggtt ggttgcaaaa cttgaactca ctttctagcc ttgactttag ttatgcagct
     4441 gacggtatct cattgactac acaagtatca cctcgcgctc ttggtaagac tcgtgatgaa
     4501 caagttgata acttggtaac aatccttgat ggttacttcg aaaacggtgg acaacacgtt
     4561 aacttgaacg ttatggactt gaacgatgtt tacgaaaaaa tcatgtcagg cgaagacgtt
     4621 atcgtacgta tctctggata ctgtgtaaac actaaatacc tcactccaga acaaaaaact
     4681 gaattgacac aacgtgtctt ccacgaagtt ctttcaatgg atgacgcctt ggatgcattg
     4741 agctaatcaa gttcttgaat aataaaaagg aaccctcggt caaacgactg agggttttgt
     4801 gcttgggata gtatgagcaa ttccttcggc gcaatatgca atgtttttgg gctctttgtc
     4861 aactgtagtg ggttgaaaaa aagctaagct
//
LOCUS       DAVBCN010000103         4832 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVBCN010000103 DAVBCN010000000
VERSION     DAVBCN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.103"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(177..1592)
                     /locus_tag="VQ497_001904"
     CDS             complement(177..1592)
                     /locus_tag="VQ497_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET2518178.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSAIPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(1605..3443)
                     /locus_tag="VQ497_001905"
     CDS             complement(1605..3443)
                     /locus_tag="VQ497_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2518179.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPDVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTPAITKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     NLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3461..4300)
                     /locus_tag="VQ497_001906"
     CDS             complement(3461..4300)
                     /locus_tag="VQ497_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HET2518180.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            complement(4600..4782)
                     /locus_tag="VQ497_001907"
     CDS             complement(4600..4782)
                     /locus_tag="VQ497_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HET2518181.1"
                     /translation="MKSQKIKVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTIL
                     RLESASIVSELVAGLR"
BASE COUNT         1623 a          934 c          629 g         1646 t
ORIGIN      
        1 ataaccaatc tatcctaagt taactattta taatcggctc tctgtaacaa aattaaatta
       61 attaatcccc ctataaaata aagaggttta gaatttgatg tcttccaaac ctctttattt
      121 tatatttaat gagatgttgc cttaaccgtg ataatatcta atcatcaata aacaaactat
      181 ttgaataagg attctccatt tgattcaatc actcctttat accaagtaaa ggacattttc
      241 ttatatcgat tgaatgtgcc atttccatca tcatttcgat caacataaat gagaccgtac
      301 cttttagaaa gttgtgcagt ggacatagaa acacagtcaa tacatcccca agacgtatag
      361 cccataattt caacaccatc ctgtagagct tcagcaactt gcaataaatg ttctttcata
      421 tactgaattc tataatcatc ttggacggtt aagttattaa gttcatcttt tattagttga
      481 tctttagcac ctaatccatt ttctactata aataatggga tttgataacg gtcataatat
      541 ctatttaaaa ttatacgtag tccaattgga tcaatttgcc atccccactc tgaagactct
      601 aaataaggat ttactaaacc accaataata ttcccttctc ctgaattata ctgtgttgga
      661 atagcagatt gagtcacact catgtaatag ctaaaggata aaaaatctac agtataattt
      721 tttaataact ctgcatcttc agctgcaaac tctatgttaa tgtcattttc cttaaaatat
      781 ctttttgcat aattcggata ataacctcta acatgcacat ctgaaaatag ataatttaga
      841 ttctcatact catgagtcgc ccatacatct tttggatttg gagtcattgg ataagctggc
      901 atagctaata ccatacaccc caccttaaac tctgaattaa tctcacgagc aatttttgta
      961 accaaactcg aggcgactaa ttcatgatgt atagcttgat ataattcttg tttcgaaaga
     1021 ttctccttag gtatatctat tcctccacta gtaaacggta attccaaaac agagtttact
     1081 tcgttaaatg taagccaata tttaacttta tctttatacc tttctaaaac tgttcgagca
     1141 aatttttcat aaaaatgaat cattctccta tcaatccatc catgatattt tcttgctaaa
     1201 tataatggag tctcatagtg tgaaagagtt acaagtggtt ctatcccgtg agcatgtagt
     1261 tcatcaaata attcatcata atatttcaaa ccagcttcgt taggttcttc ctcatctcct
     1321 tttggaaaaa ttctactcca tgcaatagaa gtacgaaaaa cattaaagcc catttcagaa
     1381 aacaaggata tatcttcctt atatttatga taaaaatcaa tacctatcaa ttttaagtta
     1441 tcttctgtag gattttctgt tgcttctcct aatccacctt tgggtaacac atcctgaact
     1501 gataagccct taccatcttc attatatgct ccctctactt gattagctgc aaccgctcca
     1561 ccccaaagaa aatcatctgg aaaaatggtc ataactttcc tccattataa tattaccagt
     1621 aattccttag aatgctcgat tgtctgatta ttaggtaata ctaatacatc tagaaaatca
     1681 ttggtattcg ttacaattac tggtgtaact gtttcgtagc ctttagtctt gattaaattc
     1741 aagttcattt caaaaatcaa ctgatttttg aaaactctgt ctccttcttc tacatgacta
     1801 ataaaacctt gaccttttag ctcaacagta tctaatccaa tatgaattag taactcaaca
     1861 ccctcatcac tcttcaatcc aattgcgtgc ttagtcggaa aaatatttgt aattttccca
     1921 tcaaatggtg cataaacctt accttcactt gggataatcg ctactccgtc tccaattaat
     1981 ttatctgaaa atgttttatc ctggacatcg cttaacggaa tgatttctcc tgatatagga
     2041 gaaaatatca tttttttatt tgaaactcca gctttaactt ctaaattgct agaactctct
     2101 tcttcatcga ttccaaatat ataagctaat acaaaggtaa taacaaccga aatgaccgcc
     2161 acaattaaag catttacaat atttgatggc acatcagaat aaataaattg aggcaacgct
     2221 atcaaagatg ggacagcaaa tagatatgct ttaacactag taagacctgc aaataatccc
     2281 gctaatccac caccaatcat agctgcataa agcggttttt tatattttaa agtcacacca
     2341 tataatgcag gttcggtaat ccctgcaagt aaggctgaga aacctgctgc aaaagcaatt
     2401 tgttttgtat tattattttt actctttaat gcaacagcca tcgaagcagc cccttgagct
     2461 aagtttgacc ctaacattgc tggaagaatt aatacgtctg gagtagcaat agatgccgcc
     2521 aaaaaaatag gtgcaaaagc ccaatgcatt ccagtcataa caataaatgg cataatagca
     2581 ccaagaatag ctaatgtaag ccatccagct acaccataca tttgcccaac tagatttgat
     2641 aatccttcac caacaattac tccaataggt ccgactacaa ctaaggcaat acagcttgat
     2701 actaataata ctagcgtagg ttgcaaaaaa ctcttagtaa tagctggtgt taatttagca
     2761 attatttttt caatatattt catcaaccaa accataataa gaattggaac gactgatgaa
     2821 ccataactag ctggtgtcac aggtgcacca aataaactaa gaggattccc tgattgcacc
     2881 atttgaacaa aatttggatg gagaagtaca cctgctacag acatagctaa tgtagatgtt
     2941 acttttaatt tttgtgatgc agaataagct aataacagcg gtaagaaata atatggagca
     3001 tccccaaaaa atgtcaaaaa agcaatagtc tgagaatctg attgcaatat accaagcatt
     3061 ggtaaaatga ttaccaagac tttcaacata cctcccccta acattgctgg aatgattgga
     3121 gtcatggaac cagcgatata ctcaatgatt ctttctaaaa tattcccttt gtgcccttga
     3181 acaactgaat cggattcaaa attgccaagt ttaacgaatt ctttataata attagctaca
     3241 tcattaccaa gtataatttg atattgtcca ttctttttca taatacctat tacatctggt
     3301 atcttcttca catcatcatc attgactaaa ttttcatctt ttaattctaa tcttaaacgt
     3361 gttacacaat gggtaactct attgacattt ttttcacctc caattacatc gaggattttt
     3421 tgtaccgtat ctttataact catggtattc tcctattcta ttaatctaaa ttttttgtta
     3481 agcgacgaat atgagccatc aaataaacta attcactaga agtcagcaaa taattgtact
     3541 cagtttgtat aaacattgcc acctgttcac cacattcata ttctctagga tatttatttt
     3601 tcattaatgc taacaagtct tcatcatcat cgtcacaata agttgtattt tcaaccaagc
     3661 gatgagcaaa taattttata tgagttatga atctataata atcaatagaa tcttggttaa
     3721 actcagtaca atagaaatct tgaatgattt tctcaattcc cataattatt tcagtgattt
     3781 tatacgactc ttgaaaatta ttttctaaat tagcattaac aaaatgtagc gctatgaatg
     3841 cagcttcgtc aattggaagt tcaataccta actctttttt tattatttgt aaagcccttt
     3901 tcccaagttc aaattcttct ttataatatt tcgatatttc ccagcgtagg ggattcgaaa
     3961 taataatccc ttctttataa cgttctatgc tcgaagaaat atgatccgtt aaattaatat
     4021 agataatttc atcaaaatta ttacccaatg ttattttacc taggttaatt attttttcgg
     4081 tgatgtacac caactccaaa ggaacgttaa taaacatctc tgtaaaacgg gtcatattat
     4141 ctctattttt gagaaaaaaa cttttctcaa tatcaattgg atttacttta tctccagttt
     4201 ttcttccaaa agctatgccc cttccaaaaa gaagaatatc gatatctttt ttactttgcg
     4261 caattacggc attgtggttt agtattcgtt ttatgatcat aaaatttccc ttttaaaata
     4321 aaaggaacct acttaaaaac atcaagaaac ttcttccgat gtgattaaat aggttcctgc
     4381 tcacttgtat tagtgataac atccttttat ttcaattttt aatggtgaca tgaagcttaa
     4441 ataattaata actctgtaac cattttcatt ttatgatatt tcaatctatt tgtcaagtac
     4501 ttgtagaaat ttttttattt tgtgtgattc gaatgtgata atggcaacag tctcactaaa
     4561 gaaatgaaaa tcatacatag aaatttttaa aaattttctc tatcgtaaac ctgccactag
     4621 ttcagaaact atactagctg attccaaacg taatatggtc atctcatcta ccttccctaa
     4681 taatggcatt gcaccatacc aaacaatagt cttattcacc aaaagaatag gcgttactat
     4741 ttttgattga gtaaaagaca ctttaatttt ctggctcttc aactgtgaac tacaccccaa
     4801 aagttagaca gaaaaaatct aacttttggg gt
//
LOCUS       DAVBCN010000104         4591 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVBCN010000104 DAVBCN010000000
VERSION     DAVBCN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.104"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(<1..1403)
                     /locus_tag="VQ497_001908"
     CDS             complement(<1..1403)
                     /locus_tag="VQ497_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HET2518182.1"
                     /translation="MFKVMRGGVDTTQVMTETVEDKVSHSITGLDILKGIVAAGTVIS
                     GTVATQTKVFTNESAVLEKTVEKTDALATNDTVVLGTISTSNSASSTSLSASESASTS
                     ASESASTSASTSASTSASESASTSASTSISASSTVVGSQTAAATEATAKKVEEDRKKP
                     ASDYAASVTNVNLQSYAKRRKRSVDSIEQLLASIKAAVFSGNTIVNGAPAINASLNIA
                     KSETKVYTGTGLDSVYHVPIYYKLTVTNDGSKLTFTYTVTYVDPTTRALGNLSKMGHG
                     YSIYNTGTSIQTMLTLGSDLGTPSDVKNYITDKDGVLVRSYNTSTMTTWGSGYTWGNG
                     AQMNGFFAKQGYGLTSSWTVPITGTDTSFTFTPYAAKTDNIRTNYFKGRGKVVESSTT
                     SQSLSQSKSLSVSASQSASASASTSASESASTSASASASTSASASASTSASASASTSA
                     SASASTSASESASTSASA"
     gene            complement(1401..1514)
                     /locus_tag="VQ497_001909"
                     /pseudo
     CDS             complement(1401..1514)
                     /locus_tag="VQ497_001909"
                     /inference="COORDINATES: protein motif:HMM:TIGR03715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KxYKxGKxW signal peptide domain-containing
                     protein"
     gene            complement(1754..2215)
                     /locus_tag="VQ497_001910"
     CDS             complement(1754..2215)
                     /locus_tag="VQ497_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2518183.1"
                     /translation="MGKERRKQGNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIA
                     ARFDCALPSVWAVLKQIKVILKKTTSFREQKPEKVSEFLDILDNLKDLPVLYIDETGI
                     NRYLYRPYAGAPRGEKVYDKISGRRFERTNEVEQKLNGSFLIRYIDSQIRE"
     gene            complement(2182..2325)
                     /locus_tag="VQ497_001911"
     CDS             complement(2182..2325)
                     /locus_tag="VQ497_001911"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2518184.1"
                     /translation="MAYSTDFKQGALDSIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KGT"
     gene            2594..2974
                     /locus_tag="VQ497_001912"
     CDS             2594..2974
                     /locus_tag="VQ497_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2518185.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            3073..3294
                     /locus_tag="VQ497_001913"
     CDS             3073..3294
                     /locus_tag="VQ497_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET2518186.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            3311..3625
                     /gene="trxA"
                     /locus_tag="VQ497_001914"
     CDS             3311..3625
                     /gene="trxA"
                     /locus_tag="VQ497_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET2518187.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            3932..4465
                     /gene="queF"
                     /locus_tag="VQ497_001915"
     CDS             3932..4465
                     /gene="queF"
                     /locus_tag="VQ497_001915"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HET2518188.1"
                     /translation="MKHQSALVVFSGGQDSTTCLFWTMQHYETVEAVTFAYGQHHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
BASE COUNT         1387 a          947 c          884 g         1373 t
ORIGIN      
        1 gctgacgcac tcgttgatgc agattcagac gctgaggtac ttgctgaagc tgaggcactc
       61 gttgatgcgg aagctgacgc actcgttgat gctgaggctg aggcacttgt acttgctgag
      121 gctgatgcgc tggttgaggc cgattcagaa gctgaggtgc ttgctgaggc tgaggcgctt
      181 tgactagcac ttactgagag tgacttagac tgtgaaagtg actgactggt cgtgctagat
      241 tcaactacct ttcctctacc cttgaagtag ttagttctaa tgttatctgt tttagcagcg
      301 taaggggtaa atgtaaagga tgtatccgtt ccagtaattg gtacagtcca agatgatgtt
      361 aatccatatc cttgcttagc aaagaaacca ttcatttggg caccatttcc ccaagtatac
      421 ccactacccc acgtcgtcat tgtagatgta ttataggatc gaaccagtac accatctttg
      481 tcagtaatgt agttctttac atctgaaggg gtaccaagat cactgccaag ggttaacatt
      541 gtttgtattg aagtacctgt attatagata gaatacccat gacccatctt tgaaagatta
      601 ccaagagccc ttgtggtagg atctacatac gtaaccgtat aggtaaaggt cagttttgaa
      661 ccatcatttg tcactgtcaa tttatagtaa attggaacat gatataccga atctaaacct
      721 gtacctgtat aaacttttgt ctcactttta gcaatgttta gacttgcatt aattgcaggg
      781 gcgccattta caatcgtatt gccagaaaaa acagcagctt ttatagaagc cagcaattgc
      841 tcgatggaat ccactgaacg cttgcgtcgc ttagcataag attggagatt gacatttgta
      901 actgatgctg cataatcact agctggtttc ttacgatctt cttcgacctt cttagcagtt
      961 gcttctgtag cggcagctgt ttgtgatcct accacagtag atgatgcaga gatacttgtc
     1021 gaagccgatg tacttgctga ttctgatgca cttgtactcg ctgaggtcga agcgctggtt
     1081 gaggctgact cagatgcaga tgtacttgcc gactctgaag ctgacaaact agttgaactc
     1141 gctgaattac ttgtagatat cgtacctaga actactgtat catttgttgc caaagcatcc
     1201 gttttctcta cagttttttc aagtactgcc gactcatttg taaatacctt cgtttgagtt
     1261 gcaacggttc cacttatgac agttcccgca gcaactattc ccttgaggat atcaagccca
     1321 gtaattgaat gacttacttt atcttctacc gtttcggtca tgacctgagt agtatcaaca
     1381 ccaccacgca tgaccttaaa caagccaaaa agagaagtcg aggcccgtag ccaatgcttc
     1441 cctgatttga ttaatttgaa ccgagttact cggtctgttt ctctatattc acccttctga
     1501 cgtctaaaaa atattttgaa atttcctttc gagtattatt ttcatgtatg gatataaata
     1561 aattaccata ctataattat agaataggga acccaaaata gcaagaaact tgaggtattt
     1621 ttgctaaaaa aaaaaagcat tttactattt tatatatata tattataaga taagtttttt
     1681 ataaagacac gcaaatgagt ataaatcagc taatttttgg tttatattcc tatttttatg
     1741 cataattatt ctttcattct ctaatttgtg aatcaatata tctgattaga aaactaccat
     1801 tgagtttttg ctccacctca ttagtccgct caaaacgccg tccgctaatc ttgtcataga
     1861 ctttctcccc tctaggagcc cctgcataag gacgatagag gtagcggttg attcccgttt
     1921 cgtcaatata taggactggt aaatctttta ggttatccaa aatatcaaga aactcagaaa
     1981 ctttctcagg cttttgttcc ctaaaactgg tcgtcttttt taaaatgacc ttaatctgct
     2041 ttaaaactgc ccatacggag ggcaaagcac aatcaaaacg ggccgcaatt tcccgtaaaa
     2101 aagcgtctgg atgagcctct acaaaggctt tcaattcttc taaagggatc ttacgctttt
     2161 tgacgactcg ctttttccgc tctaagttcc cttgtttacg tctttctttt cccacgtgaa
     2221 gagagttctg acgccaacac caaaaaactt ggctgcctcg acatgactgt gcccctcttt
     2281 gatggaatct aatgctcctt gtttaaaatc agtactatat gccatagctt tattagacca
     2341 tgcttatttt aaactaagca actatagata gagaaatgaa ataaaatcta aattcgaatg
     2401 tgataaggag ttagaaattg aaacaatagg attcccccat tattcacaca aaaataaaac
     2461 cactaaaaaa cagagcttgc cagcattctt gaaaagtagt ataattattg catgcgcaat
     2521 catcttgtga cgcagagact gcccgttaat ggactttctt ctatattatg actctaaaaa
     2581 gaaaggagac actatgacct atttggaaaa atggtttgac ttcaatcggc gtcagaaaga
     2641 aattgaaagt ctcttggaag agaccattgc ccaacagagc gagcaaagtc tgaccttgaa
     2701 agagttctac ctgctctact atctggactt ggctgaagaa aaatctctac gccagattga
     2761 cctgccagat aaacttcatc tgagcccgag cgcagtttct aggatggtgg ctcgcttgga
     2821 agctaaaaat tgcggtctac tcagtcgcat gtgttgccat caagatagac gctctagttt
     2881 tatctgcctg acaaatgatg ggcaaaagac actggcctcg ctacaaaaga ctgtcgaaga
     2941 aagcttggaa gcaggtttgg atttcttgat ttaagatgat tttaaaaaaa gttgcgtgcg
     3001 caatcatttt tcttgacatt ctcttttata aggagtaaaa taaagttagc attaaacaaa
     3061 ggagttttaa atatgattga aattacctat atagatgcca gcaagaacga aagaactgtg
     3121 actttcgagt cttatgaaga ctttgaacgt tcacaacaag cttgccttat cggcgtcgca
     3181 gactactacc ctgtccaaaa attaacttac aagggtcata atttggacta ccatgggact
     3241 tatggagata tcttcttcta tctcatgaaa caagatttaa gccaatataa ctaaaaaaag
     3301 gagaaataca atggcaaaag caattacaga tgcaacattc gaacaagaaa caaaagacgg
     3361 tttggtctta gtagacttct gggcaacttg gtgtggtcca tgtcgtatgc aaggtccaat
     3421 cttggacaaa ttgtctgaag aactttcaga agatgtcttg aaaatcgtta aaatggacgt
     3481 tgatgaaaat ccaaacacag ctcgtgcttt tggaatcatg tctattccaa ctcttctctt
     3541 caaaaaagac ggccaagttg tcaaacaagt tgcaggtgtt cacacagcag aacaaatcaa
     3601 ggccatcatt gctgaattga gctaatcaaa agagagacca agtactttct ttggtctctt
     3661 ttttcttgcc ctttgccatt tttcaaaaaa tatgctagac tatagtagat tgaaactaga
     3721 atagtacacc tctacttcta aaacattgtt aaaaatcgat ttgactgtcc tgatcgattt
     3781 gtcctgtttt tatttcattt tactatataa gtagaatatt acgatgtttg ggatatgcca
     3841 tcgctaaaaa aaaactcgat ttggtatttt ttagttccct aatgggagct ttttgcgtgc
     3901 tctgaacatt tcccattttg gaaggagtac tatgaaacat caatcagcct tggtcgtctt
     3961 tagtggcggt caagattcaa caacctgcct tttctggacc atgcaacact atgaaacagt
     4021 cgaagctgtc acctttgcct acggccaaca tcatcacctc gaaattcaaa ttactagaga
     4081 aatcgctaag gaacagggca ttcgtcacca tatcctcgat atgtctctgc tgggacaaat
     4141 cactgctcag ccagactttg cgacgattca tatttcctac attcctgaca agctctgtgt
     4201 cgagtcaaaa tccctcaaac tatatctatt tagctaccga aaccacggag atttccacga
     4261 aaactgtatc aacaccatcg ggaaagactt ggtcaacttg ctagaccctc gctatttaga
     4321 agtctgggga aaattcactc cgcgcggtgg catttcaatc gacccctact ataactacgg
     4381 taagcaagga actaagtatg agggcttggc agaacaacgc ctcttccaac acgaccttta
     4441 tccagagaaa attgacaacc gctaaagtca tactcaatga aaatcaaaga gcaaactagg
     4501 aagttagccg caggttactc aaagcagtgt ttgaggttgt agataaaact gacgaagtca
     4561 gatcaaaaca ctgttttgag gttgtggata g
//
LOCUS       DAVBCN010000105         4542 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVBCN010000105 DAVBCN010000000
VERSION     DAVBCN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.105"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(112..549)
                     /locus_tag="VQ497_001916"
     CDS             complement(112..549)
                     /locus_tag="VQ497_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET2518189.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(634..3267)
                     /gene="polA"
                     /locus_tag="VQ497_001917"
     CDS             complement(634..3267)
                     /gene="polA"
                     /locus_tag="VQ497_001917"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET2518190.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            3520..3873
                     /locus_tag="VQ497_001918"
     CDS             3520..3873
                     /locus_tag="VQ497_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HET2518191.1"
                     /translation="MILRHLGISPTNDLVAKKIFSNPEITCQFIRDMLDLPAKNVTIL
                     EGSNIHVLPSMPYSAQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQV
                     NQNLEKIRQREGDTH"
     gene            4088..4204
                     /locus_tag="VQ497_001919"
     CDS             4088..4204
                     /locus_tag="VQ497_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET2518192.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            4197..4430
                     /locus_tag="VQ497_001920"
     CDS             4197..4430
                     /locus_tag="VQ497_001920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518193.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
BASE COUNT         1378 a         1096 c          836 g         1232 t
ORIGIN      
        1 gtatctacaa cctcaaaaca gtgttttgag cagcccgcga ctagtttcct agtttgctct
       61 ttgattttca ttgagtataa cacaaaaggt agcccatcag ctaccttttt cttatgcttc
      121 ctcaatcaag cgagtatgtt ctctcttgat acagcgattc atcacgatat catcacatcc
      181 accatcacgc aaaatctctt tcgcttctaa actttcaagt cctagctgtg cccaaaaaat
      241 cttggcatca gctttgagaa aatcacgcgc cacatcgggc agaaattcac tgcgacgata
      301 aacattgaca atatctacag gaaaaggaat ttcagcgagg ctagcataag ccttttcacc
      361 caagatttcg ccacctgccg ccttgggatt gactgggatg attttatagc cccgagcctg
      421 catttccttt gtcactcgat tgctggttgt ttcttcacgg tcagacaaac ccaccacagc
      481 aagggtttta ctcgttgcga gatactgacg aatcacgcca tcacttggat tgataaattc
      541 ttgactcata gaaaacctcc tttttcatca gtatagcaca ttttgaaaag gtttgcagaa
      601 ttctactaca aaaaaggagg actagccccc tttttattta gcctcgtacc aggttgcccc
      661 ttcattctca tctgcgataa gaggaacact gagttgaatg gcttcttcca tggtttgttt
      721 caccaatttt ttcatctcta ccaattcaga tttaggcact tcaaggacga tttcatcgtg
      781 cacttgtaac agcatcttag tctgataacc acctgcaacc aaggctttat ccagctgaat
      841 catggcaatc ttgagaatat ctgctgccga accctggata ggtgagttga tagcagttcg
      901 ctccgcaaaa ccacgaatat tgaagttgcg cgaattgata tctggcaact cacggcgacg
      961 cttaaagagg gtctctacat agcccttatc acgcgcctcc cgcaccactt catccatgta
     1021 gtttttaata cctggaaaac gttcaaagta ggtatcaatg taggctttgg cttccttacg
     1081 actaattccc aaattattag acaagccaaa gtctgaaatc ccataaacca ctccaaagtt
     1141 aactgccttg gcattgcgac gatcatttgc agtcacatta tcaggacgct caatgccaaa
     1201 gacccgcatg gctgtcgaag tatggatatc tgccccctct tggaaggcct taatcaagtg
     1261 ctcatcctta gaaatatgcg ccaaaacgcg caattcaatc tgtgaatagt cagagctgag
     1321 tagcacacta tcctcccact ctggcacaaa agccttccga atcaagcgcc cttgttccaa
     1381 tcgggcagga atattttgca agtttggatc cacactagac aaacgcccgg tctgggtcaa
     1441 atcctgcata tagcgagtat gaatctttcc atcagccaaa atccagtcct gcaagccaat
     1501 tacataagta gattgaatct tagcaatttg acggtaatcc aggattttct taacaatcgg
     1561 agcaatagga gcgagacgct ccaacacatc cactgctgtc gaataacctg tcttggtttt
     1621 cttagtgtat tctagaggaa gtcccaattt ctcaaagaga agcacgccca actgcttagg
     1681 cgagttgaca ttaaactcct caccagccag ctcgtaaatc tcttgagtca gtttttcaat
     1741 gacaagctca ttttcagcct gcatctcaag caaggtctct ttcttgacca caatcccagc
     1801 aatttccatc ttggcaagaa caaaagccag aggttgctcc atatcataaa gaagctctaa
     1861 ttgcccattt tcgctgagtt tttcaagtaa aataggctct gtttctacca aaacagcaag
     1921 tttacaagct aagtgttcca agaatttctc acgttcagga atggcctttt taacaccctt
     1981 accgtagaaa gtttcatcat caaccaagta agtctgacca taaagactag cgatggtcgc
     2041 aatttcattg tcctccacag tcgaaaggag gtatttagcc aaacggatgt caaaagcagg
     2101 cgcctgcaaa tccacaccaa aacgttgcaa aagaacttta accttcttaa agtcataaac
     2161 tctcagagat gttttttcta agaaatcctt gaaaatcggg tcttgcaaca gctcaagctt
     2221 gtctgtggca tagagcttat ccccacaaga ccaggcaaat ccaaccaaat tatccgtatg
     2281 ataattctca ccaaaaagct caaagtggaa gatagactct tcactcagca tatcttgact
     2341 gatttggtca acaatagtaa aatccaaacc ctcagccaca tcagctgacg acacatttaa
     2401 agcctgcttg agctgtttga atcccatctc atcgtaaaat ttcccaagat tttcaacatc
     2461 tggaccacta tagaccaagt cctctaaacc aatcgcaatc ggtgccttgg tatcaatggt
     2521 cgctagtgtt ttagacaaaa aggcctgttc cttgtcattg atgagatttt ccttcatctt
     2581 agaagtcttc attccatcaa tattttcata aatcccctca agcgaaccat gctccagcaa
     2641 gagcttaata cccgtctttt caccgacttt ggtcacccca gggatattat ccgacttatc
     2701 acccatgagc gccttgagat cgataaactg agctggtgtg aggcccattt cttccatgag
     2761 gtaatctggc gtaaaggcct caaactcagc cacacctttc ttggaaattt caaccaccgt
     2821 atgctcatcc gtcagctgaa tcaaatcctt gtccccactg acaatggtaa tatcaaaacc
     2881 atcctgctct gctagcttat ccaacgtccc aatgatgtca tccgcctcat actgagccag
     2941 ctcatagtga cgaatcccca tatgatccag caactcacga atgaaaggaa attgctcacg
     3001 aaactcatca ggagtcttgg cccgaccacc cttatagtcc gcatacatct ctgtccggaa
     3061 ggtcgtcttt cccgcatcaa aagccaccaa aatatgactc ggctcaaccc gctccaacaa
     3121 atgactcaac atcaactgaa aaccataaat cgcattggta tgcaaaccag ccgcattctt
     3181 aaaacggtcc aactgctgat acagcgcaaa aaacgcccga aaagctacag aagacccatc
     3241 aatcaataat aattttttct tatccataca cccattataa aggaaagaat caaaaaatac
     3301 cattgggaag agctagagca agtatttttc aaactttttc cgaataaata gatagagcca
     3361 gagaatttag taaacctaga tttaaaaatg tgctataata tagtatattg aatctataat
     3421 agtacacctt gactgctaaa atatttctat aaattaattt gactttcctg atagagttgt
     3481 tcacatctta tttcaattca ctatagaagg aggaaaagga tgattctcag acatctggga
     3541 attagcccga ccaatgactt ggttgctaag aaaattttca gcaacccaga aatcacttgt
     3601 caatttatcc gcgatatgct ggacttgcca gccaaaaatg tgactatttt ggagggaagt
     3661 aacattcatg tcttgccttc catgccctac tcagcgcagg atttttatac cagtatagac
     3721 gtcttggcgg agttggataa cggtactcaa gtaattattg agattcaagt ccatcatcag
     3781 aattttttca tcaatcactt gtgggcttac ctgtgcagtc aggttaatca aaatcttgaa
     3841 aaaattcgtc agcgagaagg tgatactcac tagatctaca aacacatcgc tcctgtttac
     3901 gtcattgcta tcgtggatag taattatttc tcagatgacc tggcttttca tagctttagt
     3961 atgcgcgaag acacaacagg tgaggtattg gcgattacca acaatggaca ggaaaaccat
     4021 ctggttaaga tggcattctt ggaattaaaa aatacagaga aaccagcaaa gacaaggttc
     4081 gcaagccatg gttggagttt ttcggcaaca agccctttac ccagcaaccg caacgagcca
     4141 ttacccaagc aaatcaactg ctggactaca agagctggtc cgaggaggac aggaaaatgt
     4201 ttagtcaact acatatgcga gaagaacaag tcttgttagc acaggactat gccttggaaa
     4261 ctgctagggc tgaaggcctt gaacaaggac tagagcgtgg gaaagttgaa ggaagggcag
     4321 aaaggaaact ttttgccttc ctagacatag tacgccaagg tcttctgact tctgaggttg
     4381 ccagccagca attaggtatg tcagtatctg aatttgaggc actgttgtaa aatggctcca
     4441 taatatccat agtgggtaaa tcccctatgg atattatgga gcctattttt gtgtagaaaa
     4501 aaagtcccat atgacctata atgaaaagcg acaaaacaac tc
//
LOCUS       DAVBCN010000106         4438 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVBCN010000106 DAVBCN010000000
VERSION     DAVBCN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.106"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..119
                     /locus_tag="VQ497_001921"
     CDS             <1..119
                     /locus_tag="VQ497_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2518194.1"
                     /translation="QQGFTDRLVGYSSSIALVFTTLIGMISLVQRRVLKEDD"
     gene            121..966
                     /locus_tag="VQ497_001922"
     CDS             121..966
                     /locus_tag="VQ497_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2518195.1"
                     /translation="MKRFKPTTILEYILLTLLAGLFLFPMVWMVVSAMKPEADVYKNL
                     TSWQAFLPSLNPADWFKPYQEVFERFSIFTYLGNSIFYAGTFAIGSIIVNALAGFAFA
                     KVNFSGKKILFGFLLALLIIPVETVLIPQFTIINSLGLVNNRLAVIIPGLASVFNIYL
                     FRNFFIAIPEEILESAKMDGASIVRIFFRIMLPMSKPAVATVGVLSFISSWNDYIWPL
                     MVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTISTIPLILVYVVAQKYIVQGLGGS
                     GTGIK"
     gene            976..2295
                     /locus_tag="VQ497_001923"
     CDS             976..2295
                     /locus_tag="VQ497_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET2518196.1"
                     /translation="MINKTFTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKISQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWREKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(2503..2575)
                     /locus_tag="VQ497_001924"
     tRNA            complement(2503..2575)
                     /locus_tag="VQ497_001924"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2540..2542),aa:Lys,seq:ctt)
     gene            complement(2839..4110)
                     /locus_tag="VQ497_001925"
     CDS             complement(2839..4110)
                     /locus_tag="VQ497_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET2518197.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWIKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
BASE COUNT         1294 a          809 c          976 g         1359 t
ORIGIN      
        1 atcaacaagg ttttactgat cgtcttgtag gatattctag ctcaatcgct ttagtattta
       61 caacgttaat tggtatgatt agcttagtac aacgtcgcgt tctgaaggag gatgactaag
      121 atgaaacgat ttaaaccaac aacaatctta gaatatattc tgttaacttt attggcagga
      181 ctcttccttt ttcctatggt ttggatggta gtttcagcta tgaagccaga agcggatgtt
      241 tataaaaatt tgactagttg gcaagccttc ttaccaagtt taaatcccgc tgattggttt
      301 aaaccttatc aggaagtatt tgagcgcttt agtattttta catatctagg gaacagcatt
      361 ttttatgctg gaacgtttgc tattggttct attattgtca atgcactggc cggtttcgct
      421 tttgcaaaag tcaatttttc aggtaagaaa attttatttg gtttcttact tgctttactt
      481 attattccag tggaaactgt gttaattcca caatttacca ttatcaattc cttaggtttg
      541 gtcaataatc gtcttgcagt tattattcca ggattggcga gtgtatttaa tatttactta
      601 tttaggaatt tctttattgc aattccagaa gagattcttg agtctgcaaa gatggacgga
      661 gcaagtatcg ttagaatttt ctttagaatt atgcttccaa tgtctaaacc tgcagttgct
      721 acagttggtg ttttatcatt tatttcaagc tggaatgatt atatctggcc attgatggtt
      781 ttgacagatt cttctaaatt ttcaatgcag gttgcaatta caactattaa tacaactcaa
      841 ccagtttata tcaatcaggt aatggctgta ttaactattt caacgattcc gttgattttg
      901 gtctatgtag tcgctcagaa atatattgtt caaggtctgg gtggttccgg aactggaata
      961 aaataggata tacttatgat aaataaaaca tttacagtag aaaaagccaa tcgttttata
     1021 gcagaaaata aacatctcgt taatactcaa tataagcctg aagaacattt ttcagctgag
     1081 attggttgga tcaatgatcc aaatggattt gtctattttc gtggagaata ccatctcttt
     1141 tatcaattct atccatatga tagtgtttgg gggcctatgc actggggaca tgctaaaagt
     1201 aaggacttgg tgacttggga gcacttgcca gtggcacttg ctcctgacca agattatgac
     1261 cgaaatggtt gtttctcagg ctctgccatt gtcaaggatg atcgcctctg gctcatgtac
     1321 actggacata tcgaagaaga aaccggtgtc cgccaagtgc aaaatatggc attttcagat
     1381 gacgggattc actttgaaaa gatttcccaa aatccagttg caactggatc agacttgcca
     1441 gatgagttga ttgctgctga tttccgtgat ccaaaactct ttgaaaaaga tggacgctat
     1501 tactccgtag tagctgccaa acacaaggat aatgtgggct gtatcgttct actagggtcc
     1561 gataacctag tagaatggca gttcgaatcc atctttttaa aaggaggaga acaccaaggc
     1621 tttatgtggg aatgcccaga ttacttcgag ttagatggga aagattgcct tattatgtca
     1681 cccatgcgtt atcagcgtga gggagactca tatcataaca tcaactcatc gcttttgttc
     1741 acgggtaagg tagattggag agaaaaacgt tttatcccag aatcagttca agaaattgat
     1801 catggccaag acttctatgc gcctcaaaca ttgttggacg atcaaaatcg tcgtatcctg
     1861 attgcttgga tgcagacatg ggggcgtacc cttccaaccc atgaccaaga acacaaatgg
     1921 gcatgtgcca tgactttacc tagaattcta agattggaag atggcaaact aagacaattc
     1981 cctgttaaaa aaggccaata tcaaatccaa atagataaag attgtcatta ccacttagga
     2041 aatgatacag attatcttga atttggttat gacagtaatg cgcagcaagt ttacattgat
     2101 cgtagccatc ttattcaaaa aattctaggt gaagaagaac aggacactag tcgacggtat
     2161 gtagatattg aagctaaaga attggaagtt gttctagata aaaattccat cgagattttt
     2221 gtcaatcaag gtgaagcaag cttgactgca acttattact taacggtgcc agctgagcta
     2281 tcacgaattg attaaaaatt aagttatttc tcctaaagaa aaagttctct ttctaaaata
     2341 gtggaaagag gactttttgt gttttgggta tataagctta gtttatggta tttgtaaaat
     2401 tggtgttgga ttatgattta agctagtttt ctaaagaatt tgaaaaaaat tttatttaag
     2461 caaaaaaacc ttggttccaa ggcttttcct gttgtattta gatgccccct acagggatcg
     2521 aacctgtgac ccacggatta agagtccgct gctctgccag ctgagctaag gaggcaaaga
     2581 aaaagctgta ttggtgccga aacttcacgg tttgtattga acccgcgcaa ttaagcaggt
     2641 gggcaactcg ctctaactga agctgtttcc gtgtgagacg gcctacatgc tgttagaaga
     2701 cttttgtttc cctaataata caaaaaatag tcggtcaaca cttaagtgtg aagtcgtaca
     2761 ccacagcgtt tctatactta tatgatacca ctttttcaaa aaaaatcaag aggaaagtgc
     2821 aattttttga aaaggatttc agatttttcg caaacgatcg atagcttcct ttctttgagc
     2881 caaagcccgt tcatatttac cagtatcttc tgcttggaaa tagtgatgat tacgaatttt
     2941 ttctggcaga tagtcttgct tgatccaatt tccaggatag ttgtgtggat agagatagtc
     3001 ttgggcattc cccagttcct tgcttccact gtagtgccca tcacgcaggt gtcgcggaat
     3061 aggcaagtgc cctgatgttt tgaggtcagc aagtgcctta tccatagcta cataggctga
     3121 gttggatttt ggagaaaggg ccaaatcaat cacgacattg gcaatgagaa tgcgggcttc
     3181 ggggaaacca atcctttgtg cagcatccag agcagtcacg gtatgaatct gggcctcagg
     3241 attggctaag ccaatatctt cataggcgat aacagtcaag cgacgagcga gactaggcag
     3301 atccccagcc tcaatcaagc gggcagcata gtggagactg gcatccacat ctgagccacg
     3361 gatagacttt tgcagggcag atagaacatc atagtgaccg tctccatcct tgtccatagt
     3421 gatgtagctt ctctgaagac tattttccat gatgtctaag gtgatgtggc gaatgccctc
     3481 gtcattctca ggggtagaga gaacagccaa gtccagtgag ttaaaggcag agcgaaggtc
     3541 tccgtttgta gaggttgcga tgaaatccag cgcatcctca tctagttcta ttggaaaatc
     3601 aaaaccacgt tcagggttac ttagagctat ctgcagggcc tctttgacgt cttggttaga
     3661 cagaggttcc aactcgaaaa tttgaacacg gctacgaatg gcaggagtga cagagaagaa
     3721 aggattttca gtcgttgctc caatcatgat gaccagtcca ctttccaaga gaggcaagag
     3781 gaagtcttgc ttggtcttat ctagtcgatg aatttcgtct agcaatagga cgagaccacc
     3841 agaaaattta gcttcttccg agatttcttg cagtcgcttt ttactatcaa ctgtcgcatt
     3901 gaaagttcga aaggcatact tggtcgttcc agcgatggca gaggcaatac tggttttgcc
     3961 gattccagga gggccatata gaatcatgga ggacaggcgg ttggcttcca ccatgcggcg
     4021 gatgattttt ccaggtccga ccagatgctc ctgaccgatg acctggtcga tggttttagg
     4081 gcgcatgcga agcgcgagat tgtctggcat agcagtcctt tctaacgtgg atttgagtag
     4141 aatgactcat ctactatatc ttactatgtc cattctagcc tattagttgt gatatttcaa
     4201 tggttttgta ataatcaggg agtgagatag aatctttttt actatttaat ttaatgagga
     4261 gaggtatcag catgagagat ttgattttcc ttaaagctca aaataggata gttgagccta
     4321 aagaagtgga agtgctgact tgagaaataa tattgctgaa tgtcattgta tgaactataa
     4381 tgaaaagaga ggtgataagc aatggaaatg atggaccaag tcaggaaatt ttgatttt
//
LOCUS       DAVBCN010000107         4423 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVBCN010000107 DAVBCN010000000
VERSION     DAVBCN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.107"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(56..1066)
                     /locus_tag="VQ497_001926"
     CDS             complement(56..1066)
                     /locus_tag="VQ497_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HET2518198.1"
                     /translation="MSFLSKNGAGILACLLISIVSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIDAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKELSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            1215..2384
                     /locus_tag="VQ497_001927"
     CDS             1215..2384
                     /locus_tag="VQ497_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2518199.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            2381..3151
                     /gene="recO"
                     /locus_tag="VQ497_001928"
     CDS             2381..3151
                     /gene="recO"
                     /locus_tag="VQ497_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET2518200.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            3148..4140
                     /gene="plsX"
                     /locus_tag="VQ497_001929"
     CDS             3148..4140
                     /gene="plsX"
                     /locus_tag="VQ497_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET2518201.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            4146..4379
                     /locus_tag="VQ497_001930"
     CDS             4146..4379
                     /locus_tag="VQ497_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET2518202.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQSVGDVVKIIQGK"
BASE COUNT         1286 a          842 c         1135 g         1160 t
ORIGIN      
        1 aacatatata cggcaaggcg acgttgacgt ggtttgaaga gattttcgaa gagtgttaga
       61 aaatgccgat aagggtctgc ataccaagag tggtgaggat gatggcaatc cagcagatgg
      121 ctccaagaag aatggatttt ccactggatt tgaccatagc gaccagattg gttttgagac
      181 cgatagcact catggccatg acaataagga atttagagag ttctttgaga ggggtaaaga
      241 aactactgga cacacctaga gaggtgagta gtgtagtgag gagagaggca aggataaagt
      301 aaaggataaa aagtgggaag acttttttca gttgtaggct ttgcttgttt tcttgttggc
      361 gactttgcca gtaggagaga aagagcgtga tagggataat agccaaagta cgtgtgagtt
      421 taacaatggt tgcagactcg agggtattgc tttggtaaag actgtcccaa gcgctggcgg
      481 cagccgttac agaggaagtg tcgttgaccg cagtccctgc aaagagggcg aagccttcat
      541 tggatagatg aagccaggtg ccgagggttg gaaagatgag cgcagccaag acattgaaga
      601 aaaagataac ggaaatggct tgggctactt ccttttcctt agcatcaata acgggcgctg
      661 tcgctgcaat ggcagaaccc ccacagatag aagaacctac tccaaccaag gtagccagtt
      721 ttgtatccag tgcaaagaaa cgctggaaga ggtaggcaat aatcagagct attgagatag
      781 tggacaggat gacagggagt gaagattggc caactgcgaa gacctgcgag atattgagac
      841 caaaaccaag caagacaacg gcatattgga gcaacttctt ggaactaaag gtcaaaccag
      901 catccagttg tttataggac gagagaaagg gatgtaggag catgcctatg aaaatggcaa
      961 aaacgggcgc gccaatcaca gggaagaatc ctcctaagta ccaagatacg atagaaatga
     1021 gaaggcaggc caagatacct gctccatttt ttgatagaaa tgacataaaa acctccaaat
     1081 aaaatctatt accattatag acctataaac aggaaaagta aaatagaaag tggaaagcta
     1141 ttctctaatg tatttttgcg gtcggggggc ttttgtagta taatagagat acgttttgaa
     1201 agtaggaggt atctatggac ttaactaagc gctttaataa acagttagat aaaattcaag
     1261 tttcgttgat tcgtcagttt gaccaggtta tttcggagat tcctggggtc ttgcgtttga
     1321 ccttggggga acctgatttt acaacgccag accatgtcaa ggaggcggcc aagcgagcga
     1381 ttgatcagaa ccaatcctac tatacaggga tgagtggtct gctgactcta cgtcaggcag
     1441 ccagtgactt tgttaaggaa aagtaccaac tggactatgc tcctgaaaat gaaattttgg
     1501 ttacaattgg ggcgacagag gctttatctg cgactttgac ggctattttg gaagagggag
     1561 acaaggtact tttgccagct cctgcttatc caggctatga accgattgtt aacttagttg
     1621 gggcagaaat tgttgagatt gatacgactg aaaatggttt tgtcttgact cctgagatgt
     1681 tggagaaggc cattttggag cagggtgata agctcaaggc ggttattctc aactatccag
     1741 ccaatccgac aggaattacc tacagtcgag agcagttaga ggccttggca gctgttttac
     1801 gcaagtacga aatttttgtt gtctgtgatg aggtttactc agaattgacc tacacaggcg
     1861 aagcccatgt gtctctagga acgatgttga gagaccaggc tattattatc aatggtttgt
     1921 ctaaatcgca tgccatgaca ggttggcgtt tggggctgat tttcgctcct gcggccttca
     1981 cagcccagtt aatcaagagt caccaatact tggtcactgc cgcaaatacc atggcgcaac
     2041 atgctgcggt agaagccttg acggctggta aaaatgatgc ggagcccatg aagaaggaat
     2101 atatccaacg tcgggactat atcatcgaaa aaatgactgc tcttggtttt gagattatca
     2161 aaccagacgg tgccttctat atttttgcta aaattccagc gggctacaat caagactcct
     2221 ttgcttttct gaaggatttt gctcagaaga aggccgttgc ctttatccct ggtgcagcct
     2281 ttggacgtta cggggaaggc tacgtccgcc tatcttatgc agccagcatg gagactatca
     2341 aagaagccat gaaacgactt gaggagtaca tgagagaagc atgattcagt ctatcacgag
     2401 tcaaggcttg gtgctttaca atcgcaattt tcgtgaggat gacaagctcg tcaaaatttt
     2461 tacagagcag gttggcaaac gcatgttttt tgtcaaacac gctggtcagt ctaagctggc
     2521 gcctgttatt cagcccttgg tgctggcacg atttctcttg cgaatcaatg atgacggact
     2581 cagttacatc gaagactatc atgaggtcat gacttttccc aagattaata gtgacctctt
     2641 tgtcatggcc tatgcgacct atgtggcagc tcttgcagat gctagtttgc aggacaatca
     2701 gcaggatgct cccttgtttg cttttttgca aaagactttg gagttgatgg aagcaggctt
     2761 ggattatcag gttttgacca atatttttga aattcaaatt ttgactcgat ttggaatcag
     2821 cctcaatttt aatgagtgtg tcttctgcca tcgggttggt caggcttttg acttttcttt
     2881 caaatatgga gcctgcctct gtccagagca ttatcatgag gataagagac gttgtcatct
     2941 caatcccaat atcccctatc tgctcaatca atttcaagct attgattttg agactttgga
     3001 gaccatttcg ctcaagcctg gaatcaagca agagctacgc caatttatgg atcaattata
     3061 tgaagagtac gttgggattc acctaaaatc aaagaaattt attgattccc tagcagactg
     3121 gggacaatta ctaaaagagg aaaagaaatg aaaaaaatcg cagtagatgc catggggggc
     3181 gattacgcac ctcaggccat tgttgagggt gtcaatcaag ccctatctga cttttcagat
     3241 atcgaggttc aactttacgg agatgaagct aaaatcaagc aatatctgac agcgacagag
     3301 cgcgtcagca ttatccatac ggatgagaag attgattcgg atgatgaacc tacgagagct
     3361 attaggaata agaaaaatgc cagtatggta ttggcagcca aggctgtcaa agatggtgaa
     3421 gcagacgctg tcctttcggc tgggaataca ggtgccttgt tggcagcagg attcttcatc
     3481 gtgggtcgta tcaagaatat cgaccgtcct ggactcatgt ctaccttgcc taccgttgat
     3541 ggaaaaggtt ttgacatgct agaccttggt gccaatgcag aaaatacagc ccagcacctc
     3601 catcaatatg cggttctagg ttccttctat gctaaaaatg tccgtggcat tgcgcaacca
     3661 cgcgttggtt tgctcaacaa cggaacagag agtagcaagg gcgacccgct tcgtaaggaa
     3721 acttatgaat tactggcggc tgatgaaagt ttgaacttta tcggaaacgt ggaagcgcgt
     3781 gatttgatga atggcgttgc agatgttgtt gtggcagatg gtttcacggg aaacgctgtg
     3841 ctcaaatcca tcgaagggac agctatggga atcatgggct tgctcaagac agctattaca
     3901 ggtggtggtc ttcgagcgaa actaggtgcc ctccttctca aggacagcct cagaggtttg
     3961 aaaaaacagc tcaattattc agatgttggt ggagcggtct tgtttggtgt taaggcacct
     4021 gttgtcaaga ctcatggctc aagcgatgcc aaggctgttt atagtacgat tcgtcagatc
     4081 cgtaccatgc tagaaacaga cgtggttgcc cagactgcgc gtgaattttc aggagaataa
     4141 aagagatgac agaaaaagaa atttttgacc gtattgtgac cattatccaa gagcgacagg
     4201 gagaggactt tgtcgtgaca gaatccttga gtctgaaaga cgatttggat gcggattctg
     4261 ttgacttgat ggagtttatc ttgactctgg aagatgaatt tagtatcgaa atcagcgatg
     4321 aggaaattga ccaactccaa agtgtaggag atgtggttaa aatcattcaa ggaaaatagc
     4381 aatcggagtt ccaagtcaac ggaagtagat ggtttttaga aat
//
LOCUS       DAVBCN010000108         4304 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVBCN010000108 DAVBCN010000000
VERSION     DAVBCN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.108"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(<1..617)
                     /locus_tag="VQ497_001931"
     CDS             complement(<1..617)
                     /locus_tag="VQ497_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HET2518203.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KQTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLEN"
     gene            complement(726..1244)
                     /locus_tag="VQ497_001932"
     CDS             complement(726..1244)
                     /locus_tag="VQ497_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HET2518204.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(1471..2340)
                     /gene="rsmI"
                     /locus_tag="VQ497_001933"
     CDS             complement(1471..2340)
                     /gene="rsmI"
                     /locus_tag="VQ497_001933"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET2518205.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGTSAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQDVEEKDEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(2343..2660)
                     /gene="yabA"
                     /locus_tag="VQ497_001934"
     CDS             complement(2343..2660)
                     /gene="yabA"
                     /locus_tag="VQ497_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HET2518206.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(2700..3590)
                     /locus_tag="VQ497_001935"
     CDS             complement(2700..3590)
                     /locus_tag="VQ497_001935"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET2518207.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(3587..4225)
                     /locus_tag="VQ497_001936"
     CDS             complement(3587..4225)
                     /locus_tag="VQ497_001936"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET2518208.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVKVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
BASE COUNT         1194 a          979 c          685 g         1446 t
ORIGIN      
        1 taattctcaa gctgtgagtt cattatgatt tgtaaatccg atatatcttc agcagctaaa
       61 aacacatttt gaaatgttcc aagtccgttt tcaccaaaac tatattctct cttactatac
      121 ttactcttat tattctctat atagttagag tcaaggttct taacctcttg aagttcattt
      181 tctttacttc tgaggttaaa atcttttact tctgaagtta agttttttga cttctgtaag
      241 tcattttctt ttacctgaaa tatactcata aagtctttaa cataaatgat gttcggctta
      301 ccaagtccaa gccttactct ttcgatcagt cctattcctt tttttacatc aagctcatct
      361 aatgttttta tggcagttgg ctttgagata tttcttcttt tcataatttc ttcgacagta
      421 aaatagataa atactctgcc ttccttgtct atccagttat tcttaaatga cattcctgtc
      481 tgtttaagaa gtatggaata aaggataatt gcttctgctg ataagccccg aaattcttct
      541 ctatcaacta atatctccgg tacttttaag aagttaaatc tttccgcttc tctgttataa
      601 aaatattcaa agtccatgca agatcacctc ccttcttaaa aatttgcaaa gaaaaagacg
      661 atagtttttc ttctatcgcc tttggtaacc atttttatga aattttttaa agttaaaaat
      721 ataatttata atactatctt cttccatttt ctgattttag ttctgaaagt tccaaaaggt
      781 gcaaccgtat ttacatgaat aaacttatac acttcccaag tcgctgtttt agtcgcttca
      841 tcagcccact ttctcatatg cggttcaaat aattcttcct cactcaacga atcaatcatt
      901 gcagagatag aattaatatt ttcatttaat tttgctttca actcttgcag agataaatga
      961 gcgtaggtat ctgtgaacca ctgatataat tcaccaagtt gattccattt aaatttatcc
     1021 gatggtgttt ttacttgaag cccctttctt tcatcttctt cccatttaag aaccaagttg
     1081 gtccaaccaa cctgataagc aaggttttct gctggagttc tgtcaacttc atcagctctc
     1141 ttatctttta aattttctgg aatattatca aattctaaaa tatatttctc aaatgttttc
     1201 tctatctcag cttttagttc ttctttattt tcgtatgttc gcaaaaaatc accttctttc
     1261 tgatttatca atcctttgat ttaaaatgac cttatttttc tatttatacc ttctaagaac
     1321 gctataactg ctgctccaaa catcggtatt ccgtatgggc ttaataaaaa acttaatacc
     1381 aaagcttcta taccaagtga tacatcttta tattatcatc actaattaag ttaaacagaa
     1441 acagacagaa ttattacatc ctgtctccct ttattgtttt tcttcccaat cgtggtaggc
     1501 agcgtagagt tgactcttat tccactggta aatcttagct acttccttaa tagcttgatt
     1561 tttcttcatg ccttgctgga tacgggcttg gatttctaag aacaagtcct cctcgtcttt
     1621 ttcctccaca tcctgactgg caccttcaac aataagaagg cattcgccct tgagtggcgt
     1681 ttcagcgatg ctttccagta attcagaaat tgtacctcgt tggtattctt catagatttt
     1741 ggtcaattcc ctgaccaaaa caaccgagcg gtcaccgtag acttctaaca tattttccaa
     1801 cgtgtctgct acacgatgag gtgattcata aaaaatctgt gtttcaggat aatctttttt
     1861 agagccaaaa aattgcttct gttgacctga ttttctcggt aaaaaaccgt aaaagatatg
     1921 tggctgtggc gctaaaccac tggcaatcaa ggcagaaatt cctgcagagg tacctggaac
     1981 agtcacaact gcaatttctt cctcaatagc tgccttaact aaatcatgac cagggtctga
     2041 aatgctaggc aaaccggcat cagagacctg agcaatactt tgccctgctt tcaagaaacc
     2101 aatcaaatca ggaatttttt ccttggcatt gtgctcatga aaactgatct gcttggtgga
     2161 aatgtcaaaa tgcttgagca aaagccctgt attgcgcgta tcctcagcag caatccagtc
     2221 cacttctttc aaggtctgga tagcacgaaa agtcatatca tctagattgc caatcggcgt
     2281 tgccactaga tacagcttgc catagggaga ctgcccctta aaactttttt gaatttgcat
     2341 gcttactccc tgtataacaa ctcatcacag aacatacatt cttcgtcctg ctctcgacgt
     2401 tgtccataaa aatcattaca tacgtgaaat ccatcacggt aaatacgacg gacactttcg
     2461 cgaacatgct tggccttgac gggagcgtct gcttccacct cacccaagcg ttctcgcaac
     2521 ttactatttt ccaagcgaag agctgtattt tcttctacca agctcttgag atttttcttg
     2581 atggcttcca catcggctaa agttaccaat aattgttggg aaaaatcatc cagcgcgtca
     2641 aataattctt ttttgtccat aaaacagccc tttcctttct ttatcattca tttttgagtt
     2701 tatatttctt tcaagaccag atattccatg gcattttgaa agctgacatt agcttgccac
     2761 atttttctag cttctagtaa atcttgtaga atcactctta ctcttacctg caagaggtcc
     2821 tgcccacaga gaacttcaag aatccgtaaa acctgatcct gtttttcctt atcatctgcc
     2881 aagttggcta atttggcaac ctgtagataa ctttcttttt tcttagctac taaccaagtc
     2941 agcaggcgtt cactttcatc gaccaaggtc caaaaacttg cctgattagc caacttttct
     3001 gcttcagctc gcgattgact aaacttagct aaaagagtcg cttttttctt aacaagtccc
     3061 atttgttcta agagtaagat aagtttttct tcttgctttt taaagtggaa gatctgagtc
     3121 cgacttcgga ttgtcggtaa catcttttcc tcatcgctag tcaagaagaa aatataaact
     3181 tcactctggg gttcttcgat gaccttgagc agagaattgg ctgcgttggg atgcatttta
     3241 tccgcttgct cgatgataaa gacctgttgc tggctttcaa tccctgcttg agaaaactgt
     3301 cccaccaatt cccgaatacg ttctgtcttg atgacctgat tgactggctt aatcaaggtg
     3361 acatctggaa actcttcctg ttcaatcagc ttgcaacttc ggcatttctc acatggtaag
     3421 acgccaactt tatccgtaca aaagaggctc ttagctaaaa attgcgccat ttccaagctt
     3481 ccaaagaaac ctgaaaagag ataggcgtga ttgagctggt cttgttctaa gatacggaca
     3541 aaacggtcaa actgagctgg ttgccaagcc tttagttgat cttgtttcat ttggccaagc
     3601 ccattccgtc aaacaagaca gccttggtag tttccacaac ttgctccaaa gggagactag
     3661 catcaatctt gacaatgcga tttccctctt tatccagaag agaaaggtag ccttgacgaa
     3721 cttttttatg caagtccaac ccttccaaat ccaaacgatt aacctcgcgg tcactattag
     3781 cagcaatacg agccagccct tcttccacct cgatgtcaaa atagagtgtc aaatcgggtt
     3841 tgaggccatc tgtcgcaaac tgattgagcc agtcaatggc ttcaatatct aagccacgac
     3901 caaatccctg ataggcaaca gaactatcga taaaacgatc catgatgacc aacttgccag
     3961 cttcaagggc tggaagaact ttttccacca aatgctgtct gcgactggca atatagagaa
     4021 gtagctctgt tttagcatcc atctgagtat gacttggatc caaaatcact tcccgaatct
     4081 tctccccaat caagactccg ccaggttcac gggtcgtcag cacctttact cctttttcct
     4141 ctaaaattgg tagcagagcc tctaaaacac tggtcttgcc tgctccctct ggtccctcaa
     4201 gagagactaa aaatcctttt gacatgtcta actcatttct tttttactac ttctattcta
     4261 tcaaaaaaat aggggtttgt gacaatcttt ttgtcttctc attt
//
LOCUS       DAVBCN010000109         4287 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVBCN010000109 DAVBCN010000000
VERSION     DAVBCN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.109"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            226..1392
                     /locus_tag="VQ497_001937"
     CDS             226..1392
                     /locus_tag="VQ497_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET2518209.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESLATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTTLLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            1508..2545
                     /locus_tag="VQ497_001938"
     CDS             1508..2545
                     /locus_tag="VQ497_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HET2518210.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHVYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            2753..3607
                     /locus_tag="VQ497_001939"
     CDS             2753..3607
                     /locus_tag="VQ497_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2518211.1"
                     /translation="MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREYYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            3757..4014
                     /locus_tag="VQ497_001940"
     CDS             3757..4014
                     /locus_tag="VQ497_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HET2518212.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
BASE COUNT         1272 a          775 c          980 g         1260 t
ORIGIN      
        1 ttattcggca acctcaaaac ggtgttttga gctgacttcg tcagtcttat ccggcaacct
       61 caaagcagtg ctttgagcag cctgcggcta gtttcctaca gattttagtt ggaactcgat
      121 tcaattcatg tgacaacgtg aaaatcgtta gagcatttta tatagaatat acatgggaat
      181 gtagcttact cccattccca tatttaatag aaaaagagga actcaatgct acattataca
      241 aaagaagact tgctcgaatt gggtgcagaa atcactacgc gtgaaatcta ccaacagcct
      301 gatgtatgga gagaagcttt tgaattttat caagcaaaac gtgaagaaat tgcagccttc
      361 ctacaagaaa tcgctgataa acatgactat attaaggtta tcttgacagg tgctgggact
      421 tctgcttatg tgggagatac cttgctacct tattttaagg aagtctatga cgaacgcaaa
      481 tggaatttca atgctattgc gacaacagat atcgttgcca atccagcaac ctatttgaaa
      541 aaagatgtgg caactgtcct tgtgtctttt gctcgtagtg ggaattcgcc tgaaagtttg
      601 gcgactgttg atttggccaa atccttggtg gatgagcttt atcaagtgac gattacttgt
      661 gcagcagatg gtaaattggc tcttcaagct cacggtgatg atcgtaatct cttgctcttg
      721 caaccagctg tctctaatga tgctggattt gccatgactt ctagctttac gtctatgatg
      781 ttgacaactc tcttggtctt tgatcctaca gaatttgctg ttaagtctga acgttttgaa
      841 gttgtatcta gtcttgcccg taaagtttta gacaaggcag aagatgtcaa agagctcgtt
      901 gatttagact ttaaccgtgt catctatcta ggcgctggtc ctttctttgg acttgctcat
      961 gaagctcagc tcaagatttt ggaattaact gctggtcaag ttgcgaccat gtatgaaagt
     1021 ccagttggct tccgtcacgg tccaaaatct cttatcaacg acaatacagt tgttttggtc
     1081 tttggtacaa cgacagacta cactcgtaag tacgacttgg acttggttcg tgaagttgct
     1141 ggtgaccaga ttgctcgtcg tgttgtgctt ttgagtgatc aagcttttgg tcttgaaaat
     1201 gtcaaagaag tggcccttgg ttgtggcggt gtcttgaatg atatttaccg tgtcttccct
     1261 tacatcgttt atgcccaact ctttgcttta ttgacttcac tcaaggtaga aaataaacca
     1321 gatacaccgt ctcctacagg tacagtaaac cgtgtagtac aaggtgtcat aattcacgaa
     1381 tatcaaaagt aagacagtgt ttatgaattc ttgacaagag gatttgtaaa ttatcagata
     1441 aaccatagat tgtcagtacg ctttctatgg tttgtttgct tgagagaaat agtaaaagga
     1501 gaacagaatg aaagcataca cagagcgtgt atttggaaat gttgagggtg aggatgtctt
     1561 ggcctatcga tttgagacag acggtggcta ccaacttgag gttatgactt atggtgcgac
     1621 tatcttgcgc tatgtcgcac ctgacaaggc tggaaatttt gccaatgtta tcttgggatt
     1681 tgatgacttt gatagttatg taggcaatag tcccaagcat ggagcaagtg taggtcctgt
     1741 agcgggtcgt attgcaggtg cgacctttga gctcaatggt aagacctatg accttgaggt
     1801 taataatgct agcaactgta atcacagtgg ttcaactggt tgggattcca gcttgtttga
     1861 agttgaagaa gtaagcgatc atggcttgac tctctacaca gagcgtacag atgggacagg
     1921 agggttccct ggaaatctca agatttggat cagttatcac ttggaagaaa ctggtgccta
     1981 tgaaatcagc tacaaggtaa cgaccgatca ggatacgctg gtcaatccaa ccaaccacag
     2041 ctatttcaac ttgtctggtg atttcacgca gacgattgac cgtcatgtct tccaactaaa
     2101 cacagagggc atttactcaa tcgctcctga cggtgttcct gccaaaactc cagaagccaa
     2161 ccgtgatgtg gtcaaacacg tctacaatgg taccttgttg aaggatatct ttgcagaaga
     2221 agatgagcaa atccagctgg catcaggttt ggatcatcca tttgcccttc ctgcaggcca
     2281 tgacaatgct ggattccttt atgaccaaaa ttcaggtcgc ttcctgcttt tcaagacaga
     2341 agctccttgc tttgtggtct acacagcaaa ctttgtggat gaaagtgtca tcataggagg
     2401 tcagccaatg ctacagcaca atgggattgc tcttgaagcg caagctttac cagatgccat
     2461 tcacagtgac cttaaaggcc aagtcattct taaagctggt caaaccttca ccagtaagac
     2521 acgttatgaa cttgttgtga aataaaaaaa gctggtcaaa aagtctttaa aattaaaata
     2581 acgcatattg tcaggtgatg aaaagtttga taatatgcgt tttattatga aatgatttac
     2641 tttatattat cctgaaatgg tgttattttt gagttatctg gcttgacttt cttgatggaa
     2701 attatataat agttgtaaca atgataatta caactagaaa agaggaccat ttatgaccta
     2761 tgaatacaag agtcacattt atttggcaga gacagcttta aatgtaaagg atttggccag
     2821 tcaaacagcc ttttatcagc aagttattgg tttagaaatc ttatctcaaa ctgagacaga
     2881 gtccattcta ggccttggtg gaaaagtctt ggtacagctg attcaagcac aagagagcgg
     2941 agaagtgagg gaatattatg gtctttacca tttggccatt ctcttgccca cacgcaaggc
     3001 tttagctgat gtcttgaaac acctgacaga tttacaaatt cctcttgtcg gcggtgcaga
     3061 ccatggttac agtgaagcta tttacttgga agatttggag ggaaatggca ttgaactcta
     3121 tcgagataag ccagtttcca catgggatat tcgagaagat ggacgcatta tcggagtgac
     3181 agaggctctt gcggcgcagg acatttatga gttgggggaa agagtagagc cctttatcct
     3241 ggcagaaggt acgagaatgg ggcatattca tctttccgtc aaggatagtc gaaagtccag
     3301 ccagttttat caaaaggtgt tagggctaga ggataaattc agtgtgccta gcgctagttg
     3361 gattgcggct ggagattacc atcatcattt agcagttaac gaatggggag gaaaaggtct
     3421 ggcttcgcgt aagcaaggct taccaggttt agcctactat gtcatcgaag tcgcacacaa
     3481 aaaagaacta ttaacgattg cccaacgagc acaagaagtt gacgcaccaa tcaaatggat
     3541 gacatcgagc caattggaaa tcacagactc agatggcatc gtgacccgta ttcgtttagc
     3601 aaggtaaaat taggaatttt taataagtta taagtattgc tagtttgctt tttgaacggt
     3661 ctttggtaac agaactgcgt gagctggatg ggcgagaatt ttgacaaaga agctgattag
     3721 agatataatg aagaaaaatc gagcaaggaa aaagaaatga caaattcaaa gtatattact
     3781 tgtttgaaac gttcagaggg ccagttgtgt gggattcaaa agatgattga aggagatcgt
     3841 gactgtgctg atattgtgac gcaactgaca gcagtgagat ctagtgtaga gcgcgtgatt
     3901 gagatgataa ttaccgaaaa tcttactgaa tgtatcaatc agccactaga tgattctgaa
     3961 gctcaaaagg aacgcttaga aaaggctatc cgatacttga ttaaacggaa ataaatgaat
     4021 gttttgagac gatatgtgca aaggtctatt aactattaga atagtttaat gggagtcaaa
     4081 agaagttgta aaaaatcttt aaaattaaaa aaacgcatag tatcaggtat tgaaatgtac
     4141 tgaccccaaa aagttagatt ttttctgtct aacttttggg gtgcagttca taagaacctt
     4201 ggtaatatgc gttttattat ggggagattt atacttttcg aaaatctctt caaactaggt
     4261 cagcttcacc ttgccgtaga tatggtt
//
LOCUS       DAVBCN010000110         4285 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVBCN010000110 DAVBCN010000000
VERSION     DAVBCN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.110"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..141
                     /locus_tag="VQ497_001941"
     CDS             <1..141
                     /locus_tag="VQ497_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2518213.1"
                     /translation="LLPLPPYSPEYNPIEKTWAHIKKHLKKVLPSCNTFYEALLSCSC
                     FN"
     gene            complement(134..307)
                     /locus_tag="VQ497_001942"
     CDS             complement(134..307)
                     /locus_tag="VQ497_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HET2518214.1"
                     /translation="MNIKKRVLSAGLTFASVLLLAACGQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYTDNIVN"
     gene            complement(465..2072)
                     /locus_tag="VQ497_001943"
     CDS             complement(465..2072)
                     /locus_tag="VQ497_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HET2518215.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGIGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLVRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            2225..3709
                     /locus_tag="VQ497_001944"
     CDS             2225..3709
                     /locus_tag="VQ497_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HET2518216.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFLNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGISPADRKVAVVARQKAEETGGPALALELPNGDIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            3804..>4285
                     /locus_tag="VQ497_001945"
     CDS             3804..>4285
                     /locus_tag="VQ497_001945"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HET2518217.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
BASE COUNT         1241 a          986 c          811 g         1247 t
ORIGIN      
        1 cttttacctc ttcctcccta ctcacctgag tacaatccta ttgagaaaac atgggctcat
       61 atcaaaaagc acctcaaaaa ggtattacca agttgcaata ccttttatga ggctcttttg
      121 tcttgttctt gtttcaattg actatattat cagtgtaata gtctaataga tagttaaatg
      181 tagttggatt tccactaaag gttgatgagt aagtttttgt atctgaacct gattggccgc
      241 aagcagctaa aagcaaaaca gatgcaaaag tcaggcctgc actaaggaca cgcttcttta
      301 tgttcatctt ctttctcctt aatagtggga atttgtaaag ttaattgaat ttcgagaatg
      361 aaagtttata aaccttggtt ataaaaaaca aaggatttct gcttttatac agtcctccct
      421 tgtttttata tgatttcaat tttaaatttt tctgcaaaaa atatttatag taattccaca
      481 cagaaagcat cccatggaac taagatttgt ttttcaaaga cttcttgagc tagggtgttt
      541 tcaatcaaga cagatttgac ttttccttct actgtcaagt cttgctcttc attggacaag
      601 ttagccacaa ctaggaagcg acggtcgcca tccttacgta tataagcaaa gaccttatca
      661 gccgtatcaa gcaattcaaa gtcagctcga actagccagc tattctcctt gcgaatttgg
      721 accagtttct gataggtata gaaaatagaa tctggatttg ccagcgcttc ttggacattg
      781 atcatctcgt aatttggatt aaccgccaac caaggttgac ctgttgagaa accagcgttt
      841 ttgctctcgt cccattgcat aggggtacgg gcattgtcac gtccaataac acggatactg
      901 tccatgattt cttcaatcgg aacacctttt tcaagagcct cacgcgcata gttgagagat
      961 tcaatatctt ctacttgatc cagtgtttca aacggatagt tggtcatccc aatctcctca
     1021 ccttggtaga tataaggagt tcctctcata agatgaagca agattgcaaa ggctttggca
     1081 gatttttcgc ggtattcttg gtcatttccc cagattgaga caatacgagg gaggtcatgg
     1141 ttgttccaga agagggaatt ccagccgtcc tcaactccta actctgtctg ccatttgttg
     1201 aagatttctt ttaacttagc gatattcagc tctttttgat agtgccattt aggctgacct
     1261 tcctgatact gaagaccgat atgttcaaac tggaagacca tagacaattc ttgccccttt
     1321 ggatcagagt agagcttggc aatctctggc gttgctcccc aagtctcccc tactgtcaag
     1381 agatccttat ctccaaaggt cgcctgattc atttccttga gatagggatg gagcatagga
     1441 ccattattga ctaccttctc gtcaggaatt ttgccaatca tgtcaataac atccatacgg
     1501 aaaccaccaa tacccttatc aatccagaag ttcatcatct cataaatttt ctggcgaagt
     1561 ttttcatttt cccagttgag atccggctgt ttcttgctga aaaagtggag atagtattga
     1621 cctgactttt catcgtattc ccaagcagac ccactaaaga tagaatctaa atcgttaggc
     1681 tcatcccgcc agatatagta gtctcgctca gggctattag gattttcaca ggcctctaca
     1741 aaccaggcat gctcatccga ggtatgattg accaccaagt ccatgatgat acggatatca
     1801 cgcttcttag cttctgcaat cagctgatcc atgtcctcca tggttccaaa aatagccgca
     1861 atcgcttgat aatcagcaat atcgtagcca ttatcatcca tagggctgtc ataaacggga
     1921 gaaagccaaa ttgctgtgat tcctagcttg gctagatagt ccaacttact ggtaattcct
     1981 ggcaaatcgc caattccatc tccattacta tccataaaac tctttggata gacttgatag
     2041 actacggcat tatgccacca tttttcttgc atctgtttac ctcattattt tcataatcta
     2101 tagtctatga tagcactttt taaaattttg tgcaagcgtt tgcttaatct ttttaatttc
     2161 ttttttagct ccgtagtttc ctaacttcca attttttatt ataatagagt ccagaggtaa
     2221 aaaaatgaaa aaacaagctt ttagttctga acagtatttg aatttacaac gcgaccatat
     2281 tttggagcgc attaaccaat ttgacggcaa gctctactta gagtttggcg gtaaaatgtt
     2341 agaagatttc cacgctgctc gtgtccttcc tggttatgaa cctgacaaca aaatcaagct
     2401 cttgcaagaa ttgaaagagc aggttgaggt tgtgattgcc attaatgcta gcaacattga
     2461 acattccaaa gcacgtggcg acttgggcat ttcttatgac caagaagttc ttcgtttgat
     2521 tgacaaattc aatgaattag ggatttttgt tggttccgtt gtcattacac aatacgctgg
     2581 ccaacccgct gcagatgcct tcctcaacca acttgagaaa aacggaattg attcttatct
     2641 tcattatcca atcaaaggat atccgacgga tatggatcac atcatttccc cagaaggcat
     2701 gggcaaaaac gactacatca aaaccagtcg caacttgatt gtcgtaaccg ctcctggacc
     2761 cggttctgga aaattggcaa cgtgtatgtc caatatgtac cacgaccaaa tcaatggcat
     2821 caagtctggc tacgctaaat ttgaaacctt ccctgtttgg aatcttcccc ttcatcatcc
     2881 agttaacttg gcttatgaag ctgccacagc tgaccttgat gatgtcaaca tgattgaccc
     2941 cttccatctt caaacctatg gagaaaccac tgtcaactac aaccgtgata tcgaaatctt
     3001 cccagtgctc aagcgcatgt tggaacgtat tctcggaaaa tcaccatacg cttcaccgac
     3061 agatatgggt gtcaacatgg ttggtttcgc tattacagat gacgaggctg ctgtcgaagc
     3121 ttctaaacaa gaaatcatcc gccgttacta tcaaacagtt cttgacttca aagctgaaaa
     3181 agttggcgaa gctgccgtca agaaaattga gttgctcatg aacgacctcg gtatctcacc
     3241 tgcagaccgt aaggttgctg tcgttgcgcg ccaaaaagca gaagaaactg gcggaccagc
     3301 cctagctctt gaattaccaa atggtgacat tgtcactggt aaaaactcag aactctttgg
     3361 ccctacagcc gctgccttga tcaacgccat caaaaaatca gctgacatcg ctaaagaagt
     3421 aaaactaatc gagcctgaag ttgttaagcc aatccaagga cttaaaatcg atcatctcgg
     3481 tagccgcaat ccacgccttc attcaaatga aatcctgatt gcacttgcta tcacagctac
     3541 agaaaatcct gatgctgccc gcgctatgga agaactcggc aacctcaaag gaagcgaagc
     3601 ccactcaacc atcatcttga ctgatgaaga caagaatgtc cttcgtaaac tgggtatcaa
     3661 cgtaaccttt gacccatact accaatacga ccgcttatat cgtaagtaaa gatttcaacc
     3721 tctccagcct cggagaggtt ttctctctgt ctcaggagca agctttatga tataatgaga
     3781 gaagaaaact gaaaggattt accatgtcaa aagaagttat tgtcgaaagt tttgaacttg
     3841 accacaccat tgtcaaagca ccctatgttc gcttgattgg ggaagaaaca ggaccaaaag
     3901 gagacatcat ctccaattat gatattcgct tggtgcaacc aaacgaagac tctatcccta
     3961 ctgccggcct tcacactatc gagcacctct tagccaaact catccgtacc cgcattgacg
     4021 gcatgattga ctgttcacca tttggttgcc gcacaggctt ccacatgatt atgtggggac
     4081 gtcacaccag tgctaaaatc gcagctgtta tcaaggattc gctcaaggaa atcgctgaaa
     4141 ctactacttg ggaagatgtc ccagggacaa ccatcgaatc ttgcggaaac tacaaggacc
     4201 acagcctctt ctctgctaaa gaatgggcaa aactcatctt ggaacaaggg atttcagatg
     4261 atgcctttga acgtcatgtg attta
//
LOCUS       DAVBCN010000111         4281 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVBCN010000111 DAVBCN010000000
VERSION     DAVBCN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.111"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(173..838)
                     /locus_tag="VQ497_001946"
     CDS             complement(173..838)
                     /locus_tag="VQ497_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HET2518218.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            complement(852..2231)
                     /locus_tag="VQ497_001947"
     CDS             complement(852..2231)
                     /locus_tag="VQ497_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET2518219.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            complement(2844..3608)
                     /locus_tag="VQ497_001948"
     CDS             complement(2844..3608)
                     /locus_tag="VQ497_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HET2518220.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLDEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPSRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            3678..3839
                     /locus_tag="VQ497_001949"
     CDS             3678..3839
                     /locus_tag="VQ497_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HET2518221.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTAQNLDGAYSLYKMV
                     NPEGVEYGN"
     gene            3829..4125
                     /locus_tag="VQ497_001950"
     CDS             3829..4125
                     /locus_tag="VQ497_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2518222.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLISQGLTVTNVEGGMNAWPGQVE"
BASE COUNT         1381 a         1002 c          814 g         1084 t
ORIGIN      
        1 cccgcagcta gtttcctagt ttgatctttg attttcattg agtattactc tctcttgtca
       61 cttccttcta ttttaccata aagtccagcc tttgaagaac ttttactaga agacaagggg
      121 cttctgtctc tatttgccat cttgggcatc aaaaaagagg ggtcatccct ctttacgaat
      181 tcaatgctac tagggtatcc aaatactggt tgttgatgac tgccaaaata taggtatctg
      241 ctttcaagag gtcatctggt ccaaattcaa catccaatgg ggaattttcc tgctctcgga
      301 aacccaaaat attcagattg tatttgccac ggaggtctaa tttactcaga ctttgacctg
      361 cccaagactg aggaattttc atctccacga tagacacatt tttatccaac tgaaagacat
      421 caacactatt atggaaaaga atggtctgtg ctagagactg ccccatttca tactctggcg
      481 agataaccga gtcagctcca atcttttcta gcactttctt agcggtctga cttttgacct
      541 tagcaataac agtcggtacc cccaaactct tacagtgcat aaccgcaagc acactcgact
      601 ccagattttc acctgtcgcg actacaacgg tatcgcaggt atcaatccct gctgatctca
      661 ataattcttc atctgtgatg tcaccaatca ctccacgcgc caaaactggc tcaaactgat
      721 tgatgcgctc tgcgtggtca tcaatagcga taatattcat atcctgcttg gctagggcag
      781 ctaggacact gctcccaaaa attcccaagc ccaaaattcc aatcgtacga tctgacatgc
      841 tctttccttt cttaaccaat actaatatct gctttcatat agtgaatcat atctttcttt
      901 tctggatggt aatctgccaa gctaacaaac aaggtcaagg gaccaattcg tcccataaac
      961 ataagtggca tgataacact gagagccaat ttcccaaggt caggagtcag atttgccgtt
     1021 acaccaactg tactaagagc tgaaatggtt tcaaatatga ggtggataaa gggaggattg
     1081 cctttggctg ttatccctag cagaatcaat cctatcaaga agctcatcaa aaagataata
     1141 aagacactaa aggatttttg aaccgttcgc ggcgcaatcg ttcgtctcgc aacattggca
     1201 tgaggcaagc ctagaagctc acttcgtgca aagaccaaga ggacaaaaaa tgtcgtaatc
     1261 ttgagtcccc cagctgttcc tccaggtgcc ccacctagaa acatctgtaa gatataaatc
     1321 aaaagagtca caggatgagc ctgagtataa tctatcgtag aaaagccagc tgttcgcatc
     1381 gtcactgttt gaaaaaagct aactaaaacc ttatcggcaa cagggagatt gccaatcgtt
     1441 ccagcattgt tccactcaag aaagagagta gttgccgttc caaataacaa caaacctata
     1501 gtcaacaata gtacaagctt cgtatgaaag tgcagacgtc cttttttctt tcttcctaca
     1561 tgaccagcca aatcaaacca gaccataaaa ccaaggccgc ctgtaataat caagcctgca
     1621 atcaccagat tgaccagtaa atcggtctga aaagcaaata aactggtgct ccctaaatta
     1681 tcaaaaccgg cattacagaa ggctgagatc gctagaaaaa tggaactaaa aagaccacgt
     1741 ccccagccaa gttgaggaat aaggcgaaaa ctaagcaaaa tagctcccaa gctctcaacc
     1801 aaaaaggtcg tgagaaaaat agaatagaca aactttctca aagatcgagt ttctccataa
     1861 ctaaaactat cctgaatagt tgcacggcta cgaagactaa gcttttgctt gctctggata
     1921 tagaaaaccc caataaaggt catgagccct agaccaccga tctgaatcaa gagcaaacag
     1981 attatttggc cccatatatt ataggtgtga gctactggaa gggttgagag acctgtcaca
     2041 cagactgcag agacagcagt gaaaagatga tcaaaataag tcgctcgtga gctttcaact
     2101 tggacaaagg gcaagctcaa aagaagagag cctagtaaaa tgaccaaggc aaaactcaaa
     2161 aaaatacgac gggctggcga taaacgagat aagcctagct ctattttttc cttagataat
     2221 ttgaataaca tgtttccatt gtaccataaa aagttcgaaa tgacttagat aggataaggc
     2281 aaaatctact aacaccaagc ctaaaaaatg ccaccttttt attgatagct taggcaagaa
     2341 caatacttga tagatgtgac tataggtcaa aaaagcaaga caggagcttt taacgccagc
     2401 tactctatat gcttttcagg agaaaatttc tctttttgat tataggcaat gagaaccaag
     2461 gcatcgatta gaaagctagt cacagattgc tcaaaacact gttttgaggt tgcaaataga
     2521 actgacgaag tcagctcaaa acaccgtttt gaggttgccg gataagactg acgaagtcag
     2581 taacatatac ctacggcaag gtgaagctga cctggtttga aaagattttc gaagagtatc
     2641 atttcatctc actctaaatc ttaccaacat aaaacgcata gtatcaaggt tttcatcacc
     2701 tgatatgata cgtttttctg attttaaaga tttttcccaa cctcaacatg cttaggacaa
     2761 gaggaaggtg acctcgttta aacatatttt tgaaaagtat gagctagaca ttttatgata
     2821 aaacaaatcc tttgccagta caactaaagg tggtgaacac ttgctaaact cagttctagc
     2881 gccttattaa aagcttccga gccccagtca cgactgtcgt actggtccaa gtccgctaga
     2941 gaatctgctg tgaacaacaa ttcaccccag agaaccccac gcaattgagc tactgccgca
     3001 agagcagaac actccatctc cacaacagca cagccttctt ccttacgata agccaccttt
     3061 tcagccgttt ctcggtaaaa accgtctgtc gtccaggtca tgacttcttc ataaggaatc
     3121 cctctgtctt ccaaaacttc ctcaatagca gcaatagcct ctggctgcat ttccatataa
     3181 cgagaaggtg ccacatagtg gtaactggct ccttcatctc gcagagcgcg aacagggact
     3241 agaaaggcat tttcctctat atcagctaga acaccacagg tcccagtaga gataatctgc
     3301 tccacaccat agccaatcaa ccaatccata aactgggctg ctggagcgga gccaacagga
     3361 gcctgagcca gacagacctc ctcacccttg tagttgatga catagactgg ataggtcttg
     3421 gtagcagaaa cgaattcgcc gacacaatcc gcccctactt cccttgcata gcggtcaatc
     3481 tcctcatcta aaaatgcata aacacacttc tttggcaact gcaagtccag cccctcgtga
     3541 ttgggcatga taaccgcctg aggattgtca tcaaactcta aaataggaat cgcatgtttc
     3601 tgaatcatag cccacctcct ctaattttgt actattgtat caaaaactga caaagtgtca
     3661 aggaggaagc tgatgaactg gacaaaaaac aagcctacat cgtcagctgc cacagtggtt
     3721 tgcgcagcta tatcgcagag cctatcctca agcaagcagg atttaccgcc caaaaccttg
     3781 acggcgctta ttcactatac aaaatggtta acccagaagg agtagaatat ggaaactagt
     3841 atcagcatgg ctgactttta tggaaaatac caaaatgaaa atctagaact tatcgatgtc
     3901 cgtgaagcac atgaattcca agcaggacat gcaccaggtg ccaaaaatct tccattaagt
     3961 accttagagc aaggctacaa agaactcaaa cctgaccatg aatactatgt tatttgtcaa
     4021 ggaggagtcc gctctgcatc tacctgccaa tttctcatct cccaaggcct caccgttacc
     4081 aacgtagaag gtggtatgaa tgcttggcct ggtcaagtag aataatcaag aggaactggg
     4141 caaaaactca atttcatgag aagatgaagt aatactcaat gaaaattaaa gtgcaaacta
     4201 ggaagttagc cgcaggctgc tcaaaacact gttttaaggt tgtggatgga actgacgaag
     4261 tcagctcaaa acactgtttt a
//
LOCUS       DAVBCN010000112         4242 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVBCN010000112 DAVBCN010000000
VERSION     DAVBCN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.112"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(60..659)
                     /locus_tag="VQ497_001951"
     CDS             complement(60..659)
                     /locus_tag="VQ497_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2518223.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(660..1757)
                     /locus_tag="VQ497_001952"
     CDS             complement(660..1757)
                     /locus_tag="VQ497_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2518224.1"
                     /translation="MLERLKSIHYMFWASLIFMIFPILPVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLFWGIMLIYVAGNTAFVAVNYIWFFFFLSNLLSYHFSVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYLLGILTFVDLMTLGSVRIRLVEDLKEAQ
                     TKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQIQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVEIANQLDTASLTQEL
                     ESTASMILLELVTNIIKHAKASKAYLKLERTDQELLLMVRDDGKGFETVTENELHTVR
                     ERVLPFSGEVSVISQKHPTEVQVRLPYKERK"
     gene            complement(1757..2494)
                     /locus_tag="VQ497_001953"
     CDS             complement(1757..2494)
                     /locus_tag="VQ497_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2518225.1"
                     /translation="MKRWIALNKIEFLLTKRQSVYYLLSVGMPTAFYLFFSGMYQDTP
                     GGPANFMRDYLISMTAFSMMSTAIFSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMMVDYLVSILVVFSVGHLVRGVDMSLGSWIGAAFLLIVGSIAFVALGLTLTLLPSS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAIGKCLPTYQLMELLKTFLNEGSINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(2496..3380)
                     /locus_tag="VQ497_001954"
     CDS             complement(2496..3380)
                     /locus_tag="VQ497_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2518226.1"
                     /translation="MNMIKVQGLHKNIKSKAILKDISFEVAEGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVYIQDLPVTSSKLDYTRSYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLNNQEIDQLLQFDQQQKEQFAEKLSGGQKRLLSFILTLIGRPKLVFLDEPTAAMD
                     TSTRQRFWEIVRDLKAQGVTILYSSHYIEEVEHTADRILVLNKGELIRDTTPLAMRSE
                     EIEKHFILPLAYKEVVEKSVLVENWVQKQDALQVVTCEADAFWELLVQAGCRIQEIEV
                     NNRSLLDTIFEETQKGDD"
     gene            complement(3367..3561)
                     /locus_tag="VQ497_001955"
     CDS             complement(3367..3561)
                     /locus_tag="VQ497_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518227.1"
                     /translation="MKKAILFLFIGLSLVVWLVEMFTGWFDQSFLHQVIRGAWGLGFM
                     ISVVFPMGMEWLKGEYYEHD"
     gene            complement(3558..3764)
                     /locus_tag="VQ497_001956"
     CDS             complement(3558..3764)
                     /locus_tag="VQ497_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518228.1"
                     /translation="MEEEEMRNKMIIAVSLVVAGVMTYLMFSGLDEGFYHFPWELFAS
                     FGIMSWLIREGLKLVRDVKKELEK"
BASE COUNT         1343 a          942 c          725 g         1232 t
ORIGIN      
        1 tattgttcca ttcactaccg tcaattttag cacatagtct tcatgaaaat attatatcat
       61 cataaccaac cagattcttt tgcgatatta gctgcctctg ttcgattacc agcatccagt
      121 tttgaaagaa tattggtgac atagtttcga atagttccgt ttgagaggta aagctgatct
      181 gcgatttctt ggttggataa gccccgggcg attcccttta agacagcaat ctcttgttct
      241 gtcaacggat tgggacgggt catcaccact tccatcaatt caggcgaata ctccttgcgt
      301 ccttcgagga cggtgtgcaa ggtttgcatg aggtctgcaa tgcttcgttc ctttaataca
      361 taagcatcca ctccagcctt gaccgcacgt tcaaaatacc cagcacgctt gaaggtcgtc
      421 accacaacca cctttgtttc aagcctttct gatcgtatcc actccaagac ttcaagacct
      481 gtcttaacag gcatttctac gtcaaggatg gcgatatcta cagactcctt ttctaatagt
      541 tggattgctt cttgcccatt cttggcttga aggacagact ccacatctgg ttgaagcgtg
      601 agcaattgac acatagcatc tcgcaacata ctttgatctt ctgcgactaa tactttcatc
      661 tactttctct ccttataagg tagtcgaact tgcacttccg ttggatgttt ctgactgatt
      721 acacttactt ctcctgaaaa tggaaggaca cgttctcgaa ctgtatggag ctcattttct
      781 gttacagttt caaaaccttt cccgtcatct ctgaccatca ggagcaattc ctgatctgtg
      841 cgttctaatt ttaagtaggc tttagacgct ttagcatgtt tgatgatgtt ggtcactaat
      901 tcaagcaaaa tcatggaagc cgttgactct aattcctgag ttaagctagc tgtatctagt
      961 tgattagcta tttccacctc aattccagca atttctaaca tctttttcac agtctctagt
     1021 tcggatgtca aagttctaga cttaagattt tccacaatgg ttcgcacttc acgcattgat
     1081 tctttgctaa tctgctgtat ttctcttaat tccttttcca cttgtggata agcctgtatt
     1141 tgaaataact gcaaggctag atcagtttta acactaagca tagcaaaggt atgccccaga
     1201 ctatcatgca aatcttgacc gatacgattg cgctcatttt ctgctagcaa tagatttatc
     1261 tgggtatttt gcttagtctg agcttctttc aaatcctcga caaggcgaat tcgaaccgag
     1321 cccaaagtca tcaaatcaac aaaagtaaga attccaagca gatagactag aaactcaact
     1381 tcgattctct gaaaaatcaa aagtcgcccc acaacaagga cttgagcaag aagaaaagtc
     1441 cagacatgta aagactttaa actacgtacg ctgaaatgat aacttaagag attggaaagg
     1501 aaaaagaaaa accagatata attcacagca acaaaggcgg tattcccagc tacatagatc
     1561 agcatgatcc cccagaaaag ccaagatagg cgctggctct tagttgttaa aacacccaaa
     1621 tatgccacta caaatagaat atcaatcaat aaatgccagg cagaaagcca cccagtcact
     1681 acaggtagga tggggaaaat cataaaaatt aaactggccc aaaacatata gtgtatgctt
     1741 ttcagtcttt caagcattaa gcattctcct tatgaccttg aaggtaaatg gtcaaaccaa
     1801 acaaaactgc tgaaaaaaca agtagataaa ctgtggctga tagattgatg ctaccctcgt
     1861 ttaagaaggt cttgagcaac tccatcaact gataagttgg taggcacttc ccgattgctt
     1921 gcatccagtc tggaaataaa gagatgggca tccagagtcc acctaaaaca gccaagccta
     1981 gatagagaag attgcccacg acagacatca gctgactaga aggcaagagt gtcaaggtca
     2041 agccaagcgc tacaaaagct atacttccta ctatcagcaa gaacgcagcc ccaatccagc
     2101 ttcctaaaga catatccaca cctctgacca agtgcccaac tgagaaaaca accaggattg
     2161 agaccaaata atcaaccatc atacttgtta tctttgatag ataatattct accatattta
     2221 cagggctatg acgcaatgtt ttctgccagt tgttcatctt atcggtatgt aagacagctg
     2281 ggaatgaaaa gatagcggtc gacatcatgg aaaaggcagt catggagatg aggtagtcgc
     2341 gcataaaatt agccggtcca cctggtgtgt cctggtacat gcctgaaaag aatagataga
     2401 aggctgtcgg catccctaca gataataaat aatagactga ttgccgtttg gtcaatagaa
     2461 attctatctt gtttagtgca atccatcgtt tcatcttagt catctccctt ctgtgtttct
     2521 tcaaagattg tatccaacaa actacgatta ttgacttcga tttcttgtat cctacatcct
     2581 gcttgaacta acagttccca gaaagcatcc gcttcgcatg tgactacttg taaggcatct
     2641 tgtttttgta cccagttttc aaccaagact gacttctcaa cgacttcctt gtaagctagc
     2701 ggaaggataa aatgcttttc aatctcctca ctacgcatag ctagaggcgt cgtgtcacga
     2761 atcaactctc ccttatttaa aaccaaaatc cggtcagctg tatgctctac ctcttcaata
     2821 taatgagacg aatagagaat ggtgactcct tgcgctttta ggtcccgaac aatttcccaa
     2881 aaacgttgac gagttgaagt atccatggca gcagttggct catctaaaaa gacaagcttt
     2941 ggtcgcccaa ttaaggtcaa gataaaagat aaaagacgct tttgcccacc tgacaatttt
     3001 tctgcgaatt gctctttctg ttgctggtca aactgtaata gttgatcgat ttcctggttg
     3061 ttcaagggat ttggatagat acgttgaaag aaagcaatca actctttaac ctttagtttt
     3121 tgaacaatta cattttcttg agggagatag gatctcgtat agtctaactt agagctcgtt
     3181 accggcaaat cttggatata tacttgccca ctggtgacca atttatctcc aagcaaacag
     3241 tccaagagtg tcgtcttacc agctccattt ggcccaatca aggcgacgca ttcaccttca
     3301 gctacctcaa aggaaatatc cttcaaaata gccttacttt tgatgttttt atgtaagcct
     3361 tgtaccttaa tcatgttcat aatattctcc tttcaaccac tccattccca tagggaaaac
     3421 gacggaaatc ataaatccta atccccaggc gccgcgaata acttggtgaa gaaaggattg
     3481 atcaaaccaa cctgtaaaca tttctaccaa ccataccact agtgatagtc caataaaaag
     3541 aaagagaatc gcttttttca tttttcaagc tcctttttca catctcttac taatttcaag
     3601 ccttctcgta tcaaccaaga catgattcca aagcttgcaa acaactccca aggaaaatga
     3661 tagaatcctt cgtccaatcc tgagaacatg agataagtca tgactcctgc tactactaaa
     3721 ctcactgcga taatcatttt atttctcatc tcttcttcct ccatttcata ctacaattat
     3781 agtcttttga aatcagagga gatagaagct tctgtcacta aaaaatatga caaatgtcat
     3841 aaaaaattct gttcaaaaca agcaagatac actatacaat aaaacacaat tagataaatc
     3901 taaggcaact tcctcaaaag agatatcaaa ccccattcac actataatgt aaactaatac
     3961 ttatttaaag tcaaaagagc ggaaattttt atcgtacaaa catatatatt ataggtaaga
     4021 caaaaaactc ttcaattact ttcttaaaaa tttataggag ttataaaatc tattaaattt
     4081 caatgtggat acctaaacta cagtatttat tgaaaagact ggagacaaaa agtatacgct
     4141 gccaaaatga attgctgaaa atcaaaaaag agagaagcaa actgattctc tcttaatgta
     4201 tataatatct agttataaaa atacacactc acatatctct gt
//
LOCUS       DAVBCN010000113         4156 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVBCN010000113 DAVBCN010000000
VERSION     DAVBCN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.113"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            52..693
                     /locus_tag="VQ497_001957"
     CDS             52..693
                     /locus_tag="VQ497_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HET2518229.1"
                     /translation="MRAGVLDANDGIISIAGVVIGVASATTNIWIIFLSGFAAILAGA
                     FSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNEEFKTSAQLLT
                     NKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYRI
                     PATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(735..2723)
                     /locus_tag="VQ497_001958"
     CDS             complement(735..2723)
                     /locus_tag="VQ497_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2518230.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSSESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNEGVKEQKALTLNDHQFSVKEEFTKDFIVNHVPNQF
                     NILTADYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVNASEAEQLKVAEEYEKYLQKF
                     NAQLNTEGNYVYGSTLADASAQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFLHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(2725..3483)
                     /locus_tag="VQ497_001959"
     CDS             complement(2725..3483)
                     /locus_tag="VQ497_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2518231.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            3911..4102
                     /locus_tag="VQ497_001960"
     CDS             3911..4102
                     /locus_tag="VQ497_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518232.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
BASE COUNT         1267 a          822 c          806 g         1261 t
ORIGIN      
        1 tcagaaacaa atcacgaaat tgattcaaat tttgcaggtc gtttaaatat cctgcgtgcg
       61 ggtgttcttg atgctaacga tggaattatt tccattgctg gtgtggttat cggggttgcc
      121 agtgccacga ccaatatctg gattatcttt ttatcaggat ttgcggctat cttagctggt
      181 gccttttcaa tggctggtgg agaatatgta tccgtttcaa ctccaaaaga taccgaggaa
      241 gctgccgttt cgcgagaaaa actcttgcta gaccaagata gggaactagc caaaaaatcc
      301 ctctatgctg cttatatcca aaatgaagaa ttcaaaactt ctgcccaact cttgactaat
      361 aagatctttc ttaaaaatcc actcaaggct ctggtagagg aaaaatatgg gattgagtat
      421 gaagaattta ccaatccttg gcacgctgcc atttctagct tcgttgcctt tttccttaga
      481 agtttgcctc caatgctgtc agtgaccatt tttccaagtg attaccgcat ccctgctacc
      541 gtccttattg tcggtgtggc ccttcttctc actggttaca caagtgctag acttggaaaa
      601 gccccaacca aaacagctat gattcggaac cttgctattg gtctcttgac catgggagtt
      661 accttcctgc tcgaacaact tttcagcatt tagaatacaa gaaatacctc gattttgaag
      721 tcgaggtatc ttttttacat ttgcacaatc ttgcgataac ttcttgaagt aatcatgaaa
      781 atcagcacat aggcgatgag gaagatagcg cagatagaca aggtcacaat caacatcata
      841 gtcgtatcca gtacaccaat cacttttaaa atcagactaa gcatatggta ggcaaaggct
      901 agatgtagga aggcaaagag caaaggaagg aagaatacag ttaaaacctg tttgttgatg
      961 gtttgcttga tttgcttttg atctaaaccg actttctgca agataataaa gcgctcacgg
     1021 tcttcatatc cttcagaaat ttgtttgtag tagatgacca gaacggttcc gaccataaag
     1081 ataatggata agaaaatacc gataaagaag acaccaccaa agagggcact catctgagca
     1141 ctagcatctg ctagagtgct accatacaca tagttacctt cagtgtttaa ttgagcatta
     1201 aacttttgta agtatttttc atactcctca gcgaccttga gttgttctgc ttcactggca
     1261 tttacattca taccaccgta aaactgatta tagatagccg aatctgggaa ttgatccaaa
     1321 aaggcttgta aatcaggtac aacaaggtaa ttgtaatcag cagtcaaaat attaaactga
     1381 tttggaacat ggttgacaat aaaatcttta gtaaattctt cttttacaga aaattgatga
     1441 tcatttagag ttagagcttt ctgttcttta actccctcat tctttgcaaa gagtccgacc
     1501 tcatttcctg atagagacag tttttgacca gtcatatttt cataatcttt ttggtcaaat
     1561 accatgaaaa ctgtttttgg ttggacacgg ttttgtcctt tttcaaaaat agttaactta
     1621 gttccttctt gatttgcaat accaaagtta ctgtaacgaa gtacttcttt ctctttgaca
     1681 ctataacctt tgtcacttgc aaactggctc aagagtttgt ccaagtcttc tttttcaaca
     1741 ttttgccctg aaaccccaaa atcatgagga tttagaactt ttttaaagct ttctgaggaa
     1801 ttgaaaatgc ttgtcgctgc tgacatggtt accaaaacca ttgttgacaa gatagcgatg
     1861 gtggctagtc caactgcatt tttcttcata cggaaaatca agttagaaac agatatgaga
     1921 ttatttggtt ggtaatagta tttcttattt ttctttaaga tttggaggaa aacggtaatc
     1981 cctgcattaa acaagagata agtcccaaag ataaccagta aaacagctat gaagaaggtt
     2041 gttaaggctg taagaggatc ttttaccgta agggcaagat aatagccaat tcctaaactt
     2101 atggaaccaa gaatggtttg gagaggaagg aagcgacctt ttttctctcc actagctttc
     2161 tcacgagaga gctggagggc attcatacgg gcgattcgaa gggcattcag gaacatgagg
     2221 cctaggaaaa tcaaaccgaa gacaacaagc actgtaatga caactttcgt ctggaaggta
     2281 gcaaccagct caaccttcaa tttcattagt ttgagcagga aagcgaaaat taacttgtca
     2341 aacaaggctc caataccgat acccgctcca acagttagaa tcccaaatac cactaactcc
     2401 ttaaaggtca tactgataag atgacgcttc tccaagccca acatgccata aattcctagt
     2461 tccttggaac ggttcttcat gacaaaacta ttggcataga gaacgataat ggctgacgca
     2521 agggtgacga caaacatacc aaatccaagt gtagcctgaa tggttgttcc tccacgaatt
     2581 tcagcaatct tgggattgaa ggttagagag taaaagagat aggtgacagt gactgccaag
     2641 agaacagcca gcgcaaaagg atagtagagt ttgcggtttt taatcaagtt cgataccgct
     2701 aacttattgg ttaatcgaaa catactaatt cacctcgctt gccatgacag tcaaggtatc
     2761 agagatttct tggaacatct gacgttctgt cttctctcca cggtagattt gattgtaaag
     2821 aatgccgtct ttgataaaga gtacacgttt agctctgcta gctgctgctg ttgagtgggt
     2881 taccatgaga atggtttggc cgcgctcatt gatttcatca aagacatcaa gtaaggctgc
     2941 agatgacttg gagtcaagag ctcctgtcgg ttcgtccgca aggagaattt caggttcagt
     3001 gatgatggcg cgtgctactg ctacacgctg tttctggcca ccagaaatct cgtaagggta
     3061 cttctcttgt aattggttta ttcccagatt ctcagctgtc accaccaatt tcttcatcat
     3121 ctctgtaata ggtcttcttg acaagacaag cggaagcaag atattgtcct ttacagatag
     3181 agtatctagc aagttaaagt cttggaagac aaagcccaac ttttcacgac ggaagctaga
     3241 ggcctgtgaa tttttaatgg ttgcggtgtc agttccattc aagtaaacct gaccacgact
     3301 tggtttatcc agcatagcta gaatattgag aagagttgat ttaccagaac cagactcacc
     3361 catgatggca acgtagtcac ccttttcgac ggtaaagtga atatccttga gggcttctac
     3421 ttggttgccc tgaaaacgtg ttttataaat tttttgaacg tgttttacat ctaaaagtgt
     3481 catgagaatc tcctttctta atatcccatc aaatgaaatg tagcttgcat catagtgcat
     3541 cccagctgaa cgcgctcaat ttctttgtga cttggttttc tagttttctt atgtaagatt
     3601 tgtttcatga tgttactcct ttgttcttta tgagtctagt ttacatcaaa aaaagaccgc
     3661 ttgccataac ctaaccttac aaaagagact ttaatcttac atttttgtaa gattgggtga
     3721 aatataagca aaattactta gaaaatgatt tgatttccta tttttaaagt tgtaaaatat
     3781 aagtatgaaa aggcttactt tggaaaggag gaagcgggaa ctatcatttg agtaagctta
     3841 cctagataac ataccattca atttaacact tgcaagtcgt tcaacctata aggaaagaag
     3901 gtgcgcttta atgaaacaca aagaacatat actcatcgga cttctctatc tgctctctcc
     3961 attcatcggt cagctcttag ttgaacacac acactttatc ggtacagaat ttaccggcac
     4021 tgcttatgtt atttgctggt tatcagttgt tatcagcatc caccattttt ccaaaaatgt
     4081 attgtctcag cagcaaaaat agattaaaaa actgagttta aattactgct tttataataa
     4141 aatgaaataa gaacag
//
LOCUS       DAVBCN010000114         3963 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVBCN010000114 DAVBCN010000000
VERSION     DAVBCN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.114"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(<1..370)
                     /locus_tag="VQ497_001961"
     CDS             complement(<1..370)
                     /locus_tag="VQ497_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2518233.1"
                     /translation="MLLVLVSLVHSFGSVSIIAHTIHQKFNLKVPNYRQEEDWARMGL
                     PITRKEISNWHIKASQYYLESLYKLLREKLLEQPLLHADETSYRVLESDSHLTYYWTF
                     LSGKAENQAITLYHHDQRRSG"
     gene            complement(446..1297)
                     /gene="rfbD"
                     /locus_tag="VQ497_001962"
                     /pseudo
     CDS             complement(446..1297)
                     /gene="rfbD"
                     /locus_tag="VQ497_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647909.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
     gene            complement(1363..2412)
                     /gene="rfbB"
                     /locus_tag="VQ497_001963"
     CDS             complement(1363..2412)
                     /gene="rfbB"
                     /locus_tag="VQ497_001963"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET2518234.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPDVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLVAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGIYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQATD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            complement(2422..3018)
                     /locus_tag="VQ497_001964"
     CDS             complement(2422..3018)
                     /locus_tag="VQ497_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HET2518235.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     VLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENLEEADVSEADKHHPLLKDVKPLKKEDLE"
     gene            complement(3019..3876)
                     /gene="rfbA"
                     /locus_tag="VQ497_001965"
     CDS             complement(3019..3876)
                     /gene="rfbA"
                     /locus_tag="VQ497_001965"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET2518236.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLHRFQELLQDGSEFGIKLSYAEQPSTDGLAIGEEFISDDSVALIL
                     GDNIYHGPGLSTMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEKPEYP
                     RSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRGFAWL
                     NTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLALAQPLKKNEYGQ
                     YLLRLIGEA"
BASE COUNT         1106 a          917 c          719 g         1221 t
ORIGIN      
        1 aaccactccg acgctgatca tggtggtaca gcgtgattgc ttgattctca gctttcccag
       61 acaaaaaagt ccaatagtag gtcagatggc tatcactctc tagaacccga taagaggttt
      121 catccgcatg aagaagaggt tgttctaaca acttttctcg taaaagctta taaagggact
      181 ctaaatagta ttgacttgcc ttgatatgcc aattagagat ttccttacgt gtgattggta
      241 aacccatcct agcccaatct tcttcttggc gataattggg taccttcaga ttaaacttct
      301 gatggatggt gtgagcgata atagaaactg agccaaagct atgcaccaaa gagaccagca
      361 ccaacaacaa gataattgta catcttaagc tcctttggag ttcattatct gcctctttta
      421 ttagactaga aaatcattct actacttatc tcacttcttg tttgtaaaat tcttgcaatg
      481 catcttgcca agttggaata acaaatccag tagctttggc tttggccagg ctcatcgttg
      541 agtttagcgg acgttgagct ttggctggaa attgactgga atctactggc ttgacttcga
      601 catctgtatc tttcaaaatt tcaactgcaa aatcatacca tgttgtgtct tctgtcgcat
      661 catttgacaa atgataataa ccaaattcct tacgattttc agctaggtag atcatgaatt
      721 cagccaaggt acgagtccaa gtcggacgac cgtactggtc atttacaact gttaaagtct
      781 tatgagtttt cgcaagattt tgcatggtaa aaacgaagtt tttgccataa tttccaaata
      841 cccaggcagt acggataata tagaaattag acacatgctt ctcaactaac tcttccccca
      901 tacgcttagt gcgtccatat tctgtctgtg gatctggtcg gtcatcaact tcccactctt
      961 gtccaactgg tttcttaccg tcaaagacct agtccgtaga aatataaact agagttgcac
     1021 catgcttttc agatgctttt gcgacatttt ttgtccccat cacattgatg gcgaagtcca
     1081 actcttttcc ttcatcctct gctgcatcaa cagcggtgta ggctgcacaa tggtagacta
     1141 aagtcggttt cacctcttca aaaactttct caaccatttc ttcattggta atgtccatct
     1201 cagccacatc tactgccacg tattcttcat tacgttcatc caataaatag cgaagttccg
     1261 ttcctaattg gccatttgcc cctgtaatta aaatcatctc tagacctttc taatatgtaa
     1321 ctcttcccaa tatagcaata aaaagcagcg atttcctgct ttttatactg taataatctc
     1381 ctgagtctta gcataattgg cttcaacagc ttctttttct gctttccacc attcttggtt
     1441 atctgtatac cacttgattg ttgccttgag cccagcttca aagttggtaa attcaggttt
     1501 ccaccccaac tcatcacgga gcttgctggc atcaatcgca tagcgaaggt catgtcctgc
     1561 acggtcagtc acatgatcat aggcatccgt agcttgtccc atttccttaa ggataagttc
     1621 caaaacttcc ttattgttct tctcaccatc agccccaatc aagtaggttt caccgatttg
     1681 cccttttgtc aagattgtcc aaactcctga agaatggtca ttggtatgaa tccagtcacg
     1741 aacgttctta ccttcaccgt aaagttttgg cttaattcca cttaggatgt tagtaatctg
     1801 acgtgggatg aatttttcga tatgttgata aggaccgtag ttatttgaac agttggaaat
     1861 cgttgccttg actccaaaag aacgcaccca ggctttgaca atcaagtctg atgcggcctt
     1921 ggttgatgag tacggagagc ttgggttgta ttttgtttca gcagtaaatt tctcacctgg
     1981 tccttcacca tgacctggca aatcttctcg taaagggaga tccccataaa cttcatctgt
     2041 cgatacatgg tggaagcgaa tatcatactt acgagcagct tctaaaagag tatagattcc
     2101 aatgaagtta gtatgaataa atggcgatgg atcattgagc gaattatcat tgtggctttc
     2161 cgctgcataa tgaacgatag catctgcttg agcaaccaac ttgtctacca actccgcatc
     2221 agcaatgtca ccaacaacta actcaacacg attgcctaaa atttcctcaa tattcgcgcg
     2281 gtttccagca taagtcaact tatctaggac tgtcacgtga acatctggaa agttctcgta
     2341 aacataatgg acaaagttag aaccgataaa gccagctcca cctgtcacga taatattttt
     2401 gtattcagtc atattctttc cttattccaa atcttctttt ttcaaaggct tcacgtcctt
     2461 aagtagggga tgatgtttgt ctgcttcaga tacgtctgct tcttccagat tttcccattc
     2521 aataccaagg cttggatcag cgtagttcac aaaggcatac ttgggtttga gttcaagagc
     2581 ccagtaatca ttgaccagat agctatatga cactgtatct gataaaactt ggaagccatt
     2641 agctacgcct cgaggaacaa agattccctt gcttgcatca attactgtct gataggtatt
     2701 cccaaaggtt tcaccctcgc gtagatcaac ccaagaaccc agaactttcc ctccatctgc
     2761 tacagagatg tacttatccc aaggctctgc gtggaggcct cgaaggacat ttttacgtga
     2821 gaaggataca ttgttttgca attttccttc tgcaaagaaa gactctggaa atccaagtgg
     2881 aagcactttt tccttttgga aattttcttt aaaccagcca cgattatctc catgaacggg
     2941 gatatcaaac tccaacatgc ctggaatagc ttcaaccttg cgtgccgcaa gcgtcttacc
     3001 gaaaaaatta tctgtcatct atgcttctcc aatcaaacgg agcagatact gtccgtattc
     3061 atttttctta agtggttggg ctaaggccaa tacatcttct cgactgatat agcccatacg
     3121 gtaagcaatt tcttctaagt ttgctacctg aacattttgc atccgttgca ctgtttcgat
     3181 gtactgtgaa gcctctagta aactttcatg agttccagta ttcagccaag caaagccacg
     3241 tcccataagc tcaacagaca aatcaccacg atctaggtaa gccttgttta catctgtaat
     3301 ttccagttca ccacgagggc ttggtttaat actcttggca atttcgacaa catcgttatc
     3361 atagaaatac agtcctgtca ctgcatagtt ggagcgagga tactccggtt tttcttctat
     3421 ggaaatagca ttcatgtctg tatcaaactc aaccacacca aaacgctctg gatccttcac
     3481 ttggtagcca aaaacagtcg cacctttctc tttcttggct gctttttgaa gcattgtgct
     3541 cagaccagga ccatggtaga tattgtcgcc taaaatcaag gcaacgctat cgtcagaaat
     3601 aaactcttcc ccaattgcca aaccatctgt acttggttgc tctgcataag aaagtttgat
     3661 cccaaactca gatccgtctt gaagaagctc ttgaaatcga tgtaaatcct gtggagtgga
     3721 aataatcaaa atatccctaa tcccagccaa catcaatgtt gaaagtgggt agtaaatcat
     3781 cggtttatca taaaccggca taagttgttt tgacgcagcg cgagtcaaag gatataaacg
     3841 tgtccccgaa ccacctgcta gaataatacc tttcatagta gggtaccttt ctttgttatt
     3901 ttagagtcaa ttactattaa ttgtgtactg ctctaaaaag aaaacaatca tcccatgttt
     3961 tat
//
LOCUS       DAVBCN010000115         3886 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVBCN010000115 DAVBCN010000000
VERSION     DAVBCN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.115"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            98..1357
                     /locus_tag="VQ497_001966"
     CDS             98..1357
                     /locus_tag="VQ497_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HET2518237.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTITFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQIQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    1433..1568
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1607..2107
                     /locus_tag="VQ497_001967"
     CDS             1607..2107
                     /locus_tag="VQ497_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET2518238.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            2183..2551
                     /gene="rplL"
                     /locus_tag="VQ497_001968"
     CDS             2183..2551
                     /gene="rplL"
                     /locus_tag="VQ497_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET2518239.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKTKLEEAGASVTLK"
     gene            2738..2908
                     /locus_tag="VQ497_001969"
     CDS             2738..2908
                     /locus_tag="VQ497_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518240.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            2908..3156
                     /locus_tag="VQ497_001970"
                     /pseudo
     CDS             2908..3156
                     /locus_tag="VQ497_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            3170..3510
                     /locus_tag="VQ497_001971"
                     /pseudo
     CDS             3170..3510
                     /locus_tag="VQ497_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3503..3883
                     /locus_tag="VQ497_001972"
     CDS             3503..3883
                     /locus_tag="VQ497_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET2518241.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
BASE COUNT         1245 a          693 c          828 g         1120 t
ORIGIN      
        1 agatagaact gacgaagtca gtaaccacac ctacggcaaa gtgaatctga agtggtttga
       61 agagagtaca acttgtcttt tagaaaagga gcctataatg aaagtctttc agcatgtaaa
      121 tatcgtgact tgtgatcaag atttccatgt ttatcttgat ggaatcttag cagtcaagga
      181 ttctcaaatc gtctatgtcg gtcaagataa gccagcgttt ttagagcaag ctgagcagat
      241 tatagactat cagggagctt ggattatgcc tggtttggtc aattgtcaca cccattctgc
      301 aatgacaggt ctgagaggga tccgagatga cagcaatctc catgaatggc tcaatgacta
      361 tatctggcca gcagaatctg agtttactcc cgacatgact accaatgcgg tcaaagaagc
      421 cctgacagag atgctccagt caggaacaat aacctttaac gatatgtata atcccaatgg
      481 tgtggatatc cagcaaattt atcaggtggt gaaaacttcc aagatgcgtt gttatttttc
      541 tccgactctc ttttcttcag agacagaaac aactgctgag actataagca gaactcgatc
      601 catcatagac gaaatcttaa aatataaaaa tccaaatttc aaggttatgg tagcacctca
      661 ttctccgtat agctgcagta gagacttgct ggaagcgagt ttggaaatgg caaaagagct
      721 aaatattccg ctccatgtcc atgtggcgga gaccaaggag gagtcaggaa ttatcctcaa
      781 acgatacggc aaacgccccc ttgcttttct tgaagaactg ggttatttag atcatccgtc
      841 cgtatttgct cacggggtcg aattaaacga gagagaaatt gaacgcttgg catcttctca
      901 agtggctatc gcccacaatc ctatcagtaa cctcaaactg gcatcaggaa ttgctccaat
      961 tatccagatc caaaaagcgg gagtagcagt cggaattgcg actgactcgg ttgcttccaa
     1021 taacaatcta gatatgtttg aggaaggaag gactgcagct cttcttcaga agatgaaaag
     1081 tggggatgcc agccagtttc caatcgaaac agctctcaag gtactgacaa tcgaaggggc
     1141 taaggtcctt ggaatggaaa atcagatagg aagtctggaa gtcggcaagc aagcagattt
     1201 tctggtcatt caaccacaag ggaaaattca tctccaacct caggaaaata tgctgtctca
     1261 cctggtttat gcagttaaat ctagtgatgt agatgatgtt tatatcgccg gagaacaggt
     1321 cgttaagcaa ggtcaagtcc tgacagtaga actttaaaag aaaaatcacg aaaaatttta
     1381 aaaaaagttt gcaaaaatct tgcattcttt ttttgactat gctatactta tatacggttt
     1441 aaaaaaactg cctaagacag taggggagct cgactcataa atatcctacc gaggacaaga
     1501 cgtatcatgt aaaaagaagc gtattgtact ttcgtgtcta ggtttgggcg cgtttttctt
     1561 tttgaaaaat tccccaagca aaataattac ggaggtgaac acactaatga gtgaagcaat
     1621 tattgctaaa aaagcggaac tagttgacgt agtagctgaa aaaatgaaag ctgctgcatc
     1681 tatcgtcgtt gtagacgctc gtggtttgac agttgagcaa gatacagttc ttcgtcgcga
     1741 gcttcgtgga agcgaagttg agtataaagt tattaaaaac tcaatcttgc gtcgtgcagc
     1801 tgaaaaagct ggtcttgaag atcttgcatc tgtatttgtt ggaccatctg cagtagcatt
     1861 ttctaatgaa gatgttatcg caccagcgaa aatcttgaac gacttttcta aaaacgctga
     1921 agcacttgaa attaaaggtg gtgcaatcga aggcgctgtc gcatctaaag aagagattct
     1981 tgcacttgca actcttccaa accgcgaagg acttctttct atgctccttt ctgtacttca
     2041 agcgccagtg cgcaacgttg ctcttgcagt caaagcggtt gcagaaagca aagaagacgc
     2101 ggcttaatct taagctacac agcgtagcct agctacgaaa aaaactatta taaaatttaa
     2161 aacttatttg gaggaaataa caatggcatt gaacattgaa aacattattg ctgaaattaa
     2221 agaagcttca atccttgaat tgaacgacct tgtaaaagct atcgaagaag aatttggtgt
     2281 aactgcagct gctcctgtag ctgttgctgc agctgatgca gctgatgctg gtgctgctaa
     2341 agattcattc gacgttgaat tgacatctgc aggcgacaaa aaagttggcg ttatcaaagt
     2401 tgtacgtgaa atcactggtc ttggtcttaa agaagctaaa gaacttgttg acggtgcacc
     2461 agcacttgtt aaagaaggcg ttgcaactgc agaagctgaa gaaatcaaaa ctaaattgga
     2521 agaagctgga gcttcagtta ctcttaaata atagagtatc gcaatttgat tgcggaatcc
     2581 ctatttacca gtctttgaga gcgataagcg attgatagaa actgataaat tgatttggtt
     2641 tcctgccaaa aattctttgg caggattttt gttttaataa actttcgggg gtagacacta
     2701 gattgtctac cccttttttt gtgtttcgga ggtaattatg caaaaggaag gacagtcatc
     2761 acgtcaattc gtgaagagtc atctcatgac tatcatggga gttgatattg taaaagcaac
     2821 tcagttagtt gatgaaatgg aacaagtagg tttaatccga tttgatgaat ttggaaatgt
     2881 tggaatttta gtcttggagg gacaataatg aagtgtatta ccgctaatca atatcagaca
     2941 tcagagcgtt attataagct ccccaaactt ttatttgaga gtgaacggta taagaatatg
     3001 aaactggagg ttaaggtggt ttactcagtt ttaaaagatc ggttggagtt gtctttgagc
     3061 aaaggttgga ttgatgagga tggggctatt tatttgattt attccaattc aaatttgatg
     3121 gcacttttag gctgttcaaa gtcaaaatta ctctccatgt gagtttgaag tgacattttt
     3181 agatgattac cataaaaaac ataactaccc actattttac gaatcctatc ttcaaaacgt
     3241 tatggaattc cttgaaagtc aagacataaa gaatggggtt gatgcctttg tagatgatca
     3301 tcaaaatctc gtttttgttt tatatggaca aggctatcga gccgagggaa aagagggaat
     3361 acttacaacc caagtaactg taaaagctta tgatgaagac aagaaaccga ttaacttcgc
     3421 aaatttatta gattccttaa tttattagat tccttaatcg tgtcagaata tcaaatggaa
     3481 ccgaatcttt gggaggtctc ccatgattga tctctatcta agtaaaaata gacaaagaaa
     3541 tcaacttctt ttagatttct tccaaaacta tggcatagag gtatcttgtc attcagtttc
     3601 tgaaatgaca aaggacaaat taattgagat gatgagctat tcttcagatt gttttgagtt
     3661 tttatctccc aatttattac gatttaagaa ccgtgacaat ctaagattaa cggatttcgt
     3721 tgagataatt ttaaaaaatc ctgaactaac catcagactt cctcttgcgg tttcaaataa
     3781 gcgagtttat ccaagtctga atttggaaga ggctagagct ctattgccaa gagatacgaa
     3841 acaattgatt tacatggcgc aaacacatta tttatctaac taaagg
//
LOCUS       DAVBCN010000116         3478 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVBCN010000116 DAVBCN010000000
VERSION     DAVBCN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.116"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            65..1305
                     /locus_tag="VQ497_001973"
     CDS             join(65..404,404..1305)
                     /locus_tag="VQ497_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2518242.1"
                     /translation="MVKLIDRYGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSIS
                     LDYALPNQGMLSFWPAQYKKNGYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENE
                     RLRTEVAYLKKFKRVRGKGRSPRARKAETVREMVSGGFRLDFLLETARLARSTYYYQL
                     KQLDGVDKDKEIKTEIQGIDNEHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLT
                     ARIRRKRKYSSYQGEIGKKAENLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPV
                     LDGFNSEIIAYHLSTSPNLEQVKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLE
                     SKGIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNN
                     KRIKVKLKGLSSVQYRTKSFG"
     gene            complement(1540..2148)
                     /locus_tag="VQ497_001974"
     CDS             complement(1540..2148)
                     /locus_tag="VQ497_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HET2518243.1"
                     /translation="MQIKFIDKVSNLIMLNLLYVASVVTVIAIGSGESALIATLIKIV
                     RHEESYPYRDFANSFFKDYWKNLGAALISNLPILILLFSLFFLPYIPLPIYIISILRH
                     IGVIYIILHLIATTFLIPLIGRYNNTLKNSLHNSIMLAYKHFFIAVLIRIIEIIPVLL
                     FFILQNQLLVWITLMIFILPSITKYANAFLYNFIFSKYEKLN"
     gene            2314..2484
                     /locus_tag="VQ497_001975"
                     /pseudo
     CDS             2314..2484
                     /locus_tag="VQ497_001975"
                     /inference="COORDINATES: protein motif:HMM:NF013577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            2473..3084
                     /locus_tag="VQ497_001976"
     CDS             2473..3084
                     /locus_tag="VQ497_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2518244.1"
                     /translation="MTKDILELESQKMSSDTFIDEIKNNYLSIVESTRKLIDGRQIEL
                     AIKLIREANQILMIGVGSSGNAAREFESSLLRIGIISKTVIDTHFQLMHTALLKDNDL
                     IIAFSLSGSTKEVEETLLNAKRKNVKIISITNYSSRNIAKLSDCVLLTSKKESYLEGG
                     SLMAKASQLFIIDVICTRLSLINYEDTICKKEEIASLLSNKVE"
BASE COUNT         1314 a          443 c          620 g         1101 t
ORIGIN      
        1 gcaaggacaa agcttcaaac agctttcaaa aagatttggt gtggatgttt ctggtctaaa
       61 gtacatggtg aaattaattg accgttatgg catagaaatc gttaaaaaag ggaagaatcg
      121 tcactattct tctaaattga aacaagaaat gatggataaa gccttactgg aaggttgttc
      181 acaaagaagt attagtcttg attatgccct cccaaaccaa ggaatgcttt cattttggcc
      241 ggcacaatac aagaaaaacg ggtatactat tgttgagaaa acaagaggga gaccagctaa
      301 aatgggacgt aaacggaaga agacttggga agaaatgaca gaactagaac gactccagga
      361 ggagaatgaa cgcttacgga ctgaggtggc ctacctaaaa aagttaaaag agttagagga
      421 aagggacgaa gccctagagc gagaaaggca gagacagtta gagaaatggt ttcaggagga
      481 tttcgactag attttcttct tgaaacagca cgtttagctc gctcgactta ctattatcag
      541 ttgaaacaac tggatggggt tgataaagat aaagagatta aaaccgaaat tcagggcatt
      601 gataatgaac ataaaggcaa ctatggctat cgtcgaatcc atttagaact aaggaatcgt
      661 ggttttgtgg tcaatcataa gaaagttcaa cggttgatga gaattcttgg tttaacggct
      721 cgaattcgcc gtaaacggaa gtattcttcc taccaaggag agattggcaa gaaagcagag
      781 aatctcattc aacgccagtt cgaagcatcg aggccaatgg aaaagtgcta tacagatgtg
      841 acagagtttg ccattccaaa tagcacacag aaactctatt tatcaccagt tttagatggt
      901 tttaacagtg aaatcattgc ttatcatctt tctacttcgc caaacttaga acaagtgaag
      961 agtatgttag aacaggcctt tacagagaaa tactatgaga atacgattct ccatagtgac
     1021 caaggctggc aataccaaca cgattcttat catcggttcc tagagagtaa gggaattcaa
     1081 gcatccatgt cacgcaaggg caacagccca gacaacggca tgatggaatc tttctttggc
     1141 attttaaaat ccgaaatgtt ttatggctat gagaagaact ttagatcttt agaaaacctt
     1201 gaacaagcta ttgtggacta cattgattac tacaacaaca aacgaattaa ggtaaaacta
     1261 aaaggactta gttctgtgca gtacagaact aaatcctttg gataaattat ttgtctaatt
     1321 ttttggggtc agtacagtat taaataccat gatcttatgc gttttataat aaaaaatctg
     1381 ttcttgtttt atttttagaa aatttactaa agactaaaaa tattatgaat aatttaaaaa
     1441 atagtcaggt tatatagttt tttgaaatta aaattatttt atcgttgaaa ttagttaaaa
     1501 atttttataa aatcaagatg caaggctatg agatgttctc tagtttaatt tttcatactt
     1561 cgagaatata aagttatata aaaatgcatt agcatatttt gttattgagg gaagtataaa
     1621 aatcattaaa gttatccaaa ctagtaattg attctgtaat ataaagaaga gtagaactgg
     1681 gataatttca ataattctaa taagaacggc aataaaaaaa tgtttatagg ctaacatgat
     1741 tgaattgtgt agagaatttt ttaaagtgtt attatatcta ccgattaaag gaattagaaa
     1801 tgttgttgct attaaatgaa gtatgatata tattactcca atatgtcgta agatagaaat
     1861 gatatatata ggtaatggta tgtagggtag gaagaacaaa ctgaataata agattaaaat
     1921 tggtaaatta gatattaatg ctgctcctaa atttttccaa taatctttaa aaaaactgtt
     1981 tgcgaagtct ctatagggat aggattcctc gtgtcgtaca atttttatta gagttgctat
     2041 tagggcactt tcacctgatc cgatagcaat aactgttact actgacgcta catagagtag
     2101 atttaacatt ataagatttg aaactttatc aataaatttt atctgcatct ttaactcctg
     2161 ctgtttttat acttataata tcacatcatt tgagtttcat caactttgaa tacattaaga
     2221 aagaatataa ctatgaggaa aggaaaaaat tattatgaat aaatccaaaa aatataaaga
     2281 aagaattcat tatgaatacg ataatcttac taaatcagag aaaaaattgc tgactatatt
     2341 ttagttagag atagtgaaat agtttatgaa acgtcttcag aaattgttag taaagtaaaa
     2401 gttggagagg gaactcttaa tcgattttgc aaaaagattg gatactctgg ttttcaggaa
     2461 ctaaaattaa aaatgacgaa agatatatta gagttagaat ctcaaaaaat gagtagtgac
     2521 acatttattg atgaaataaa aaataattat ttatctattg tggaaagtac aagaaaatta
     2581 attgatggta gacaaataga attagctata aaattaataa gagaagcaaa ccagatttta
     2641 atgattggtg tgggttctag tggaaatgcg gctagagaat ttgagagtag tttgttaaga
     2701 attggaataa taagtaagac ggtaattgat acccattttc aactcatgca tactgcgtta
     2761 ttgaaagata atgatttgat aatagcgttt agtttatctg gttccacaaa agaagttgag
     2821 gaaactttat taaacgcaaa aagaaaaaat gttaaaatta tttcaataac aaattattcg
     2881 tccagaaata tagcaaaatt atctgactgc gttcttttga catcaaaaaa agaatcttat
     2941 ttagagggtg gtagtttaat ggctaaggct tctcaactat tcataattga tgtaatatgc
     3001 actagactct ctttaataaa ttatgaagat actatatgta aaaaagaaga gattgcttct
     3061 ttgctgtcaa ataaagtgga ataaatcaat aatttttaaa ttttaataaa aattttttaa
     3121 aataaattaa aaaacgatcc taaaagggtc gtttttttgc taaagtttta aaaataaaca
     3181 ataattaaga ttatagtgcg ttgaatctat attaggacat aacaattctt aaatcattgt
     3241 tagaaattag tttgaatttt ccaatcaatt tgttcgattt ttacatcatt gcattatatc
     3301 acgtgactat taaccttgtt ttctcctaaa attgattttc ttcttttctt atcttgtcca
     3361 ctcgaaacaa gtattgtaag aatttgatta tttttgaaag tacttttaat atatttgaga
     3421 tagttaaaaa agatttgaaa ctaaattcca aattagaaaa agacttgaaa tgcttaaa
//
LOCUS       DAVBCN010000117         3192 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVBCN010000117 DAVBCN010000000
VERSION     DAVBCN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.117"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(381..914)
                     /locus_tag="VQ497_001977"
     CDS             complement(381..914)
                     /locus_tag="VQ497_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HET2518245.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(1003..1779)
                     /gene="recX"
                     /locus_tag="VQ497_001978"
     CDS             complement(1003..1779)
                     /gene="recX"
                     /locus_tag="VQ497_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HET2518246.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINANQLSGDKGPYVLTQKLAQKGISKSTIEEILNDFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYFDAKIAFDELDSQVDEETTQELIFKELDK
                     QYTKYARKYEGYKLKQRLTQVLARKGYDFSDIASALREYL"
     gene            1817..3172
                     /gene="rlmD"
                     /locus_tag="VQ497_001979"
     CDS             1817..3172
                     /gene="rlmD"
                     /locus_tag="VQ497_001979"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET2518247.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
BASE COUNT         1028 a          562 c          581 g         1021 t
ORIGIN      
        1 atttcatttt actatactgt tacgaaatta gatatataaa tgaaaacttg cctcaaggga
       61 ggttttttgc tagtagaaaa gcgatagaca gaaattgttg gactcttaaa aaaatgaagt
      121 tttgctcaaa agagcttctt aaattaaaac gagcaattat cagttcaaat ctgtttcaaa
      181 agaagaacgg ataaattaat cgatgctttg tttttgagtt aggaagttaa gcatgccaaa
      241 ctgagtcgta ggaataaatt tctttataga aatgtgttgt aactctgcta taatggattt
      301 attccttttt gtgtttacac aatttatttt atactaccaa aaaaacaagg tcaggatttt
      361 gttcctgacc tttgacaact ttaccgattc tttagttcta catagcgctt gtaccaaatg
      421 tttacatagg cttctgagaa aggaccacgt ccattgttaa tccaatcaac aagaattttg
      481 acatgttctt ttaaaatata gtccaagtca tcagaataat tcattttgcg tttgtgacgc
      541 tcgtactctt caacgtccaa gagacgtttt tccccatctg taaaaatttt aacatccaaa
      601 tcgtaatcaa tatacttcag tgcttcttca tccagatagt aggggctagc catattgcaa
      661 tagtaagaag ttccattatc acgaatcatg gcaatgatat taaaccaata tttcttgtga
      721 aagtaaacaa tagccggttc tcgagtgacc caacgacgac catcactttc ggtaacaagt
      781 gtatgatcgt tgacaccaat aatggcgttt tctgttgttt ttagtaccat ggtgtcccgc
      841 caagttcggt ggagactccc atcatgctta taactttgaa ttgtaataaa gtcgccttct
      901 tttggaagct tcataactaa ccaactttct acaatttata agtttatcat ttactattgt
      961 accataaaat tacctaaaat ctgtgaattt cacttggaaa tattaaagat attctctaag
     1021 agcgcttgct atatccgaaa aatcgtagcc ctttcgtgct aaaacttgag ttaaacgctg
     1081 cttcagtttg tatccttcat actttcgggc atacttagta tattgcttat caagttcctt
     1141 gaagatgagt tcctgagtcg tttcttcatc aacttgacta tccaattcgt caaaggcaat
     1201 tttagcatca aaataagaga agcccttgtt agtcaagttc tggataatct tatcttgcag
     1261 ggcacgagct ggaagttttc cctcatattt tttcaatagt ttattggcta cacgttgagc
     1321 aacttccgaa aaatcaaaat cattcaagat ttcttctata gtagattttg aaattccttt
     1381 ttgtgctaat ttctgagtca gtacataagg tcccttgtct cctgaaagtt gattggcatt
     1441 gatgatagca taagcgtact gaccatcatt aatccactta tcttctttaa gattagcaat
     1501 aacttgagaa acgatgtttt tatcaatatc gtattttttc agatattctc tgacttcttt
     1561 ttcagtgcgt gctttaaagg ataagtggta gagggccaga ttcttaccat aagaaaattg
     1621 agcaaagtct tgaatctctt tcaattcctc ttcgcttatc accttatctc tcgataacat
     1681 aaaacgaaca attgtatctt cggtgatata gcatttgtcg ccattatcaa gctccatcag
     1741 atagagtctt tttttctttt caagttttgt gattttcata gctctattat aactcaaaat
     1801 gtgataagat aggggtatga atctgaaagt gaaacaaaaa ataccattaa aaatcaagcg
     1861 catgggaatt aacggtgagg gaatcggctt ttaccaaaaa acattagtct ttgtaccagg
     1921 agctctcaaa ggcgaagata tctattgtca gattacttct attagacgca actttgttga
     1981 agcaaaatta ctgaaggtca acaagaagtc taaatttcga attgtgccat cttgtactat
     2041 ttataatgaa tgcggaggct gccaaatcat gcacctgcat tatgataagc agctggagtt
     2101 caagacggac ttacttcatc aagcgctgaa aaaatttgct cctgcaggat atgaaaatta
     2161 tgaaattcgt ccaactattg gaatgcagga accaaaatat tacagagcta agttacaatt
     2221 tcagactcga aaatttaaaa atcaggtcaa ggcgggctta tatgcacaaa actctcacta
     2281 tttagtagag ttgaaagact gcctggtaca agataaggaa acccaagtga ttgctaatcg
     2341 cttagcagaa ttacttactt atcaccagat tccaatcacg gatgagagaa aagttctagg
     2401 tgtccgtact attatggtcc gacgcgcgag aaagaccgga caggttcaga ttattattgt
     2461 tacaaaccgc cagcttaatt taactcaatt ggtaaaagag ttggttaaag atttcccaga
     2521 agttgtgaca gtagctgtta atacaaatac agctaaaacc agtgagatat atggtgaaaa
     2581 gacagagatt atctgggggc aagagagtat tcaagaaggt gtactcaatt atgaattttc
     2641 actatcccct cgagcttttt atcaactaaa tcctgagcaa acagaagtcc tctatagcga
     2701 agcagtaaaa gcgctggatg ttgataaaga agaccatttg attgacgctt attgtggagt
     2761 tggaacgatt ggatttgcct ttgcaaagaa agtaaaaaca ctcagaggta tggatattat
     2821 tccagaagct attgaagatg ccaagcgaaa tgctaaaaga atgggatttg acaatactca
     2881 ttatgaagct ggaacggcag aagagattat tcctcgttgg tacaaggaag gctaccgagc
     2941 agatgctttg attgttgacc caccacgtac aggtctggat gataagttat tagatactat
     3001 tcttacttat gtaccagaaa aaatggttta tatttcttgt aatgtttcga ccttggctcg
     3061 tgatttggta cgcttagtag aagtctatga tcttcattat atccagtcgg tcgatatgtt
     3121 cccacataca gctcgaactg aagctgttgt aaaattaata aaaaaagttt aaaaaaggtg
     3181 ttgacaaaac at
//
LOCUS       DAVBCN010000118         3093 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVBCN010000118 DAVBCN010000000
VERSION     DAVBCN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.118"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            370..1716
                     /gene="gdhA"
                     /locus_tag="VQ497_001980"
     CDS             370..1716
                     /gene="gdhA"
                     /locus_tag="VQ497_001980"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HET2518248.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
     gene            complement(2222..2392)
                     /locus_tag="VQ497_001981"
     CDS             complement(2222..2392)
                     /locus_tag="VQ497_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518249.1"
                     /translation="MKKENEYVILTIASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(2405..2983)
                     /locus_tag="VQ497_001982"
                     /pseudo
     CDS             complement(2405..2983)
                     /locus_tag="VQ497_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(3000..>3093)
                     /locus_tag="VQ497_001983"
     CDS             complement(3000..>3093)
                     /locus_tag="VQ497_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET2518250.1"
                     /translation="MFSTTYRNHRKRFGLRMNLIVGIINHELGF"
BASE COUNT          985 a          570 c          510 g         1028 t
ORIGIN      
        1 gcttggattt cttctttagt tgatttcata tcactattat ataatgattt ttgattttag
       61 tctggtataa atattgcttt cctccaaaat ggtcataatt ttactagcaa atctaacata
      121 tcacggataa attaacaagt gatttctgaa ttgctaaaca ttttcttttc ttatagcata
      181 ccatacttta agattttgtc tttgagaaag atatttccaa gaaaaacgtt cgttttttta
      241 tctattttca aaaaaaccaa aaactgaatt gaaagaattt ttagaaaatt tctgtttttt
      301 ctttacagtg aaaaaaaatt ctgctataat gtcttatgta ataaaatatg acaataaaag
      361 gagaacgata tgacatctgc taaagaatat atccaaagcg tgtttgaaac tgtgaaagct
      421 cgtaacgggc acgaggctga attcctccaa gctgttgaag aatttttcaa cactttagaa
      481 cctgtatttg aaaaacaccc tgagtatatc gaagaaaata tcttggcacg tattactgag
      541 cctgagcgcg tggtttcttt ccgtgttcct tgggttgacc gtgatggaaa aattcaagta
      601 aaccgtggtt accgtgttca attcaactca gctgttggac catacaaagg tggacttcgt
      661 ttccacccaa ctgtaaacca agggatcttg aaattcctcg gatttgaaca aatctttaaa
      721 aatgtcttga ctggacttcc tatcggtgga ggtaaaggtg gatcagactt cgatcctaaa
      781 ggtaaaacag atgctgaagt gatgcgcttc tgccaaagct tcatgactga attgcaaaaa
      841 cacatcggac catcacttga cgtacctgct ggtgatatcg gtgttggtgg acgtgaaatt
      901 ggttaccttt acggtcaata caaacgtctt aaccaatttg atgctggtgt cttgactggt
      961 aaacctcttg gatttggtgg tagcttgatt cgtccagaag caactggtta cggtttggtt
     1021 tactatactg aagaaatgct caaagctaac ggtaacagct ttgctggtaa gaaagtcgtt
     1081 atttcaggtt ctggtaacgt tgctcaatat gctcttcaaa aagcaactga actcggtgca
     1141 actgttattt ctgtttctga ctcaaatggt tatgtcatcg atgaaaatgg tatcgacttc
     1201 gatcttttgg ttgatgttaa agaaaaacgt cgtgctcgtt tgactgagta tgcagctgaa
     1261 aaagcaactg caacctacca cgaaggtact gtatggactt acgctggaaa ctatgacatt
     1321 gctcttccat gtgccactca aaacgaaatc aacggtgaag cagctaaacg tttggttgct
     1381 caaggcgtta tctgtgtatc tgaaggtgcc aacatgccga gcgaccttga tgccatcaaa
     1441 gtttacaaag aaaatggtat cttctacgga cctgcaaaag ctgccaacgc tggtggtgta
     1501 gccgtttcag ctcttgaaat gagccaaaac agtcttcgcc tctcatggac tcgtgaagaa
     1561 gttgatggcc gtctcaaaga catcatgaca aacatcttca acacagctaa aacgacttca
     1621 gaaacatacg gtcttgataa agactacctt gcaggagcta acattgctgc ctttgaaaat
     1681 gtagcaaatg ccatgattgc acaaggtatt gtttaagatt tatttactca atcctcaatc
     1741 actacgattg gggattttta gcttgaatta aaaaattgga aaatctaatt aaataaaatg
     1801 aaatgaaata aaaactgaac aaattgattg cggaattcaa accaatttct aacatacttc
     1861 tagaaattaa tttgactttt ctaatcgatt tgttcatatc ttatttcaat ttactatact
     1921 tacttaaccc tttttatact caatgaaaat caaagagcaa actaggaagc gagccgcagg
     1981 ctgtacttga gtacggcaag gcaaagctga cgtggtttga agagattttc aaagagtatt
     2041 aaacaaaaag aaaaaccgct actcctaaga atagcgattt aatcatgttt ttaagctact
     2101 actgtagttg ctctattatt taagtgttat gaataacttc aatatcttta aagctttttg
     2161 ttagatttat aatttcaact atagccatct ctactcctta gtttacagca aaaaattatg
     2221 cttaattctt attgtttttg taaacaatca gcgaacccaa tactattcca ttcaacaagc
     2281 ctaaagaaat accatattca actggaaaat ctaaaaaaat tgcaaacact attccaatca
     2341 tcacccctag tgaggctatt gttaaaatta catattcatt ttctttcttc attttcttta
     2401 cacctcattt cctttttgta tataagctgt agtactctcc accaagagtt attaagtact
     2461 tatgatttcc tgactcaaca atcttaccac ctttcattac aaaaataata tccgcatcct
     2521 taatcgttga caatctatga gctataatta tttgagtaca accctgactt tgtatatact
     2581 ttgttattct ttcctcatta atagtgtcta atgcactagt tgcttcatct aaaattacaa
     2641 tactaggatt atttattaat gcacgtgcca gtgctatctg ttgcctttgc ccacctgaaa
     2701 tatttgaccc catctctgag atgatagtat taaatttcat cggcatagcc atgatttcat
     2761 catagatttg aactgcttta caaacttcct ctatcttttg tgaagtaact tcgtgcttta
     2821 aagttatatt atcaagaata cttcggttca ataaaaatga atcctgtgga actactccta
     2881 aattttgact taatattttt ttatctattt gattaatatt tataccataa aataaaactt
     2941 ttcctgtatc aatcttatat aatcctgatg gtatttttga taatagactt cctgcaaaac
     3001 tagaatccta gttcatgatt gataatacca acaatcaaat tcatccgtaa tccgaagcgt
     3061 ttacgatgat ttcgataggt tgttgaaaac att
//
LOCUS       DAVBCN010000119         2946 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVBCN010000119 DAVBCN010000000
VERSION     DAVBCN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.119"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(106..306)
                     /locus_tag="VQ497_001984"
     CDS             complement(106..306)
                     /locus_tag="VQ497_001984"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518251.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDKTDEVSSKHGFEVVDETDEVS
                     NHTYGKATLTRIEEIFEEYKSS"
     gene            complement(284..478)
                     /locus_tag="VQ497_001985"
     CDS             complement(284..478)
                     /locus_tag="VQ497_001985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518252.1"
                     /translation="MSSKKHPLGEKRNHTGSKLRQGFWMVFRLGVNNWGFKEIENKKP
                     SKNSIAGENEIILNENQRAN"
     gene            540..1886
                     /locus_tag="VQ497_001986"
                     /pseudo
     CDS             540..1886
                     /locus_tag="VQ497_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
     gene            complement(2286..>2946)
                     /locus_tag="VQ497_001987"
     CDS             complement(2286..>2946)
                     /locus_tag="VQ497_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518253.1"
                     /translation="IIRKVQEPEFWKEVNLSDLEKIRLAIRDLLQFLDKTDRKPYYVN
                     FEDRILSTVHETTAFLQVNDLRSYNEKVEHYLKTHLDEESISKLYHNKKLTSDDMLAL
                     EKLLWEKLGSKADYQSHYENKAIPRLVREIIGLDRESANRIFSKFLSDENLNARQISF
                     VKLIVDYIVENGFLETKVLTQEPFKSYGSVQLLFQHQLPVLRNIVQIIELINNRAGEA
                     A"
BASE COUNT          881 a          614 c          522 g          929 t
ORIGIN      
        1 acaataagag aacgactcaa aattaaaaag ctaaagttcc acaattggaa atctctagct
       61 tttttgtggt cgagaactat tttgtcccag gctctttgct ttatcctagg aagatttata
      121 ctcttcgaaa atctcttcaa tccgcgtcaa cgtcgccttg ccgtaggtat ggttactgac
      181 ttcgtcagtt tcatctacaa cctcaaaacc atgttttgag ctgacttcgt cagtcttatc
      241 tacaacctca aaacagtgtt ttgagcagcc tgcagctagt ttcctagttt gctctttgat
      301 tttcattgag tattatttca ttttctcctg caattgaatt cttgctcggc tttttgtttt
      361 ctatttcttt aaaaccccaa ttattcaccc ctaatctaaa aaccatccag aatccttgcc
      421 ttagcttaga tcctgtatgg tttctttttt cacccaatgg gtgtttttta ctagacaaaa
      481 aagaatttcc cctttatggt ataagtgtag aaaaaaacac aaaaagaaag gaaactcaca
      541 tgaacagttt accaaatcat cacttccaaa acaagtcttt ttaccaacta tctttcgatg
      601 gaggtcattt aacccagtat ggtggtctta tcttttttca ggaacttttt tcccagttga
      661 aactaaaaga gcggatttct aagtatttag taacgaatga ccaacgccgc tactgtcgtt
      721 attcggattc agatatcctt gtccagttcc tctttcaact gttaacaggc tatggaacgg
      781 actatgcttg taaagaattg tcagctgatg cctactttcc aaaattgttg gaaggagggc
      841 agcttgcttc acagccaacc ttatcccgtt ttctttccag aactgacgag gaaacagtcc
      901 atagtttgcg atgcctcaac cttgaattgg tcgaattctt tttacagttt caccagctaa
      961 accaactcat tgtagatatc gattctaccc atttcacaac ttatggcaag caagaaggtg
     1021 ttgcttataa cgcccactat cgtgctcatg gctatcatcc tctttatgct ttcgagggga
     1081 agacaggtta ttgtttcaat gcccagcttc gtcctggtaa tcgttattgt tctgaagagg
     1141 cagacagctt tatcacacct gttttagaac ggtttaatca acttctcttt cgaatggata
     1201 gtggctttgc gacccccaaa ttatacgatt taattgaaaa aacagggcaa tactaactca
     1261 taaaactcaa gaaaaatact gttctgagcc gtcttggaga cctttccctc ccttgcccac
     1321 aggatgagga cttaaccatc ttgccccact ccgcctactc agaaactctc tatcaagcag
     1381 gatcttggtc gcacaagcgt cgtgtctgcc agttctctga acgaaaagaa ggaaacttgt
     1441 tctacgatgt tatttctctc gttacaaata tgacgagtgg aacaagccaa gaccagtttc
     1501 agctttatcg tggacgtggt caagccgaga atttcatcaa ggagatgaag gagggatttt
     1561 ttggcgataa aacggatagt tcaaccttaa tcaaaaacga agttcgtatg atgatgagct
     1621 gtatcgccta caatctctat ctttttctca aacatctagc tggaggtgac ttccaaactt
     1681 taacaatcaa acgcttccgc catctttttc ttcacgtggt aggaaaatgt gttcgaacag
     1741 gacgcaagca gctcctcaaa ttgtctagtc tctatgccta ttccgaattg ttttcagcac
     1801 tttattctag gattagaaaa gtcaacctga atcttcctgt tccttatgaa ccacctggaa
     1861 gaaaagcgtc gttaatgatg cattaaagaa cagtcgagat gaaaaaatcg tgtgacgcac
     1921 caagggagga gtctgccctt ttgaggaaat ctagcgagga aaaacgatac tggaacagca
     1981 gaaagtaaaa ctgacctcat gaggaggaag aaagtggctc atgaggtcag gggttttgta
     2041 agttacatct agttgagaga ggtatgaatg atttgggtta aaatcaaagt agctcttttg
     2101 ttaataactc gatcaacaaa catcgtggta caagtatcta ctttgaaatt tatcaaccac
     2161 ttaacaactg atactgtatt ctaggaaaac gatgacattc ttcctaataa aacttctcat
     2221 atatagcata aatttctact ctttttaatt ttaaatgagt ctcagcatgg caatcacttt
     2281 agaatttaag ccgcttctcc agctcgatta ttgataagtt caatgatttg aacaatatta
     2341 cgaagtactg gtagttggtg ttggaagagt agttgaacag aaccataaga tttaaacggc
     2401 tcttgcgtta acactttcgt ctctaaaaaa ccattttcta caatgtagtc tacaatcaat
     2461 tttacaaatg aaatctgcct ggcattaaga ttctcatccg acaaaaattt agagaaaata
     2521 cgattggcag actctctatc taagccaata atctcacgaa ccaatctcgg aattgcctta
     2581 ttttcataat gactttggta gtctgcttta ctacctaatt tttcccaaag caatttttca
     2641 agtgcaagca tatcatcaga tgtcaacttt ttattatggt atagcttaga aatggactcc
     2701 tcatccagat gagttttcaa ataatgctca actttttcat tgtaagaccg aagatcgttg
     2761 acctgcaaaa atgctgtggt ctcgtgaaca gtggagagta tacgatcttc aaagttaaca
     2821 tagtagggtt tacggtctgt tttatccaaa aactgtaata aatctcgaat agcaagacga
     2881 attttttcca aatcagacaa gttaacttct ttccaaaatt caggctcctg tactttcctg
     2941 ataatt
//
LOCUS       DAVBCN010000120         2891 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVBCN010000120 DAVBCN010000000
VERSION     DAVBCN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.120"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(91..1140)
                     /locus_tag="VQ497_001988"
     CDS             complement(91..1140)
                     /locus_tag="VQ497_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2518254.1"
                     /translation="MKDLFLKRKQAFRKECLCYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEAIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(1137..1868)
                     /locus_tag="VQ497_001989"
     CDS             complement(1137..1868)
                     /locus_tag="VQ497_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2518255.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            1936..2346
                     /locus_tag="VQ497_001990"
     CDS             1936..2346
                     /locus_tag="VQ497_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET2518256.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMNATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            2356..2643
                     /locus_tag="VQ497_001991"
     CDS             2356..2643
                     /locus_tag="VQ497_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518257.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
BASE COUNT          967 a          649 c          549 g          726 t
ORIGIN      
        1 tctctacgga ccaaccatta tcctgtagta acaaaaaaga agctgagaca gactgaactc
       61 aacttctttt ttagtgtcgt atcagcaatg ttagtcctgc atctgacgtt ttacctgata
      121 aggcaaatac aagactagta aaaccaaacc agctcctagt aaggctagaa gggccagttt
      181 ttcttggaag gtaatcaacc caacaactaa ttccacaagt aaaacaaaac tggtcaatcc
      241 tcgaactacc tcctgtaagc ctttttcctt ttgccccttg tccagcggaa agagttgggt
      301 caaatactgg tagtcaaagg catgatagag ggccagcaac tggaagagca agaggtagtt
      361 aaagagaact accactgctg tcgcaatcca agcttgctcg ataaaaacct gcgccagtac
      421 ggaaagtaat agaagacgaa gactgagagc aaagaggtcg ccatttcgca gataagaacg
      481 cagatagaga ttttgccaaa tcttcccagg caccttctga acagccttta agataaagtc
      541 cagataggca cgacgcttaa cgctgtttga aattcccttg acctgcgtaa agagggcaaa
      601 gaaacgaagc aagacttgct tacgcttgct ttcttgagaa ataacatagt cccagtccag
      661 tccagtttca gtaaaaaatt tgctggcctt ttgacgaaag tggaaatatt ttcctacccc
      721 caataaaagc acatatagca gaaaaactgg caagccataa cccattgcta aaaataaagg
      781 cgcaaataac agcaagaaaa gggtctgtac aaagagccaa aagactaggg aaatgccagt
      841 ttgacgcttg agatggagct tgattgcctc ttctccaact aagagaaaga gcttgtctgg
      901 agcctccata taggtggcaa ttcctcccca aagtaagagt aaaacagacg taattcctac
      961 aaacaaaagg ataggccaat gattttcagg aaaatgttgt aagagttgac tgtactggta
     1021 ggctagaaag cccaacagga caagcaggaa caagacaaag tggtcattga gcacatagcg
     1081 cagataacaa agacactcct tacgaaaggc ctgctttctc tttaaaaaca agtctttcat
     1141 agatcctcct ctttggtcag agccaagtaa atatcattca aactagcctc aggcatatca
     1201 aaggcttcac gtagttgcag gagattgcct ttggcacgca cctctccctt gtgaagaatg
     1261 acaaaggcat cacacatctt ttccgccgaa tccagcacgt gggtactcat gagaatagac
     1321 ttgccctttt gcttctccac ttccaaaagc tgaatcaaat cagaaatagc cagcggatca
     1381 agaccaagga aaggctcatc cacgatgaaa agacttggat ccaccacaaa agcacagata
     1441 atcatgacct tctgcttcat cccttttgaa aaatgaacag ggaaccagtc taatttctgt
     1501 tccaaacgga acatttttaa caagggctct actcgttcga aagccacttt ttgctcaata
     1561 ccgtaagcca tagcaaccgt ttcgatatgc tctctgaggg tcaattcctc atacagacta
     1621 ggcgtctcag gaatgtagcc aatctgcttg cggtagctag tcgcatctcc ttgcagagtc
     1681 aggccattga tattgatgga gccactataa ggtgccaaca gaccgataat ctcattgatc
     1741 gtcgttgatt tcccagcacc attgagacca atcaaaccga ccaactgccc actttcaaca
     1801 gtaaaggaca catctttcaa aacaggaaca tgaacatagc cacctgtcag gtttttaatt
     1861 tctaacatat tttctccaaa tctggtataa tgtagctata ttatatcaaa attcagtaca
     1921 gtagaggtag attttatgtc agattgcatt ttttgtaaaa tcatcgcagg ggaaattcct
     1981 gcttcaaaag tatatgaaga tgagcaggtc cttgcctttc ttgatatctc tcaagtaaca
     2041 ctaggacaca ccttggtcgt gccaaaagaa cactatcgca atcttttgga gatgaatgct
     2101 acgagcgcca gccaactctt tgcccaagta ccaaaagtag ctcaaaaagt catgaaagtc
     2161 actaaggctg ctggtatgaa tatcatttcc aactgtgaag aagtcgctgg tcaaacagtt
     2221 tttcatactc acgttcacct tgtgcctcgc tacagtgctg acgatgacct caagattgat
     2281 tttatcgccc acgaaccaga ctttgacaaa cttgctcaag tcgctgaaac catcaaaaac
     2341 gcttaaggag ttgctatgaa attatccaac ctactgctat ttgcaggagc tgcagccgga
     2401 agttatctgg ttacaaaaaa tcgccaaacc atcacagatg aagtcttgaa taccactgac
     2461 cgcgttcaag ctatcaaaga cgatgtggat attatccaaa atagcctgca aatcatcgac
     2521 cagcaaaaag aacttatcaa ggaataccaa gaagacttga cttacaagtt taaggtcttg
     2581 gaaaaggata tccagactag actagctgtg ataaaagaaa tgcagggaac tgaagataag
     2641 taaaaagagc cccccggctc ttttttaaaa cgtaaacaag ttatagtgaa ttgaatttag
     2701 aatagcacat catagtttct aaagcattgt tagagattac tttaaaatcc tcttatcaat
     2761 ttgttcatat tctatttcaa tctactatat atatcccatc aactatgcat cataatttag
     2821 gtaactcata ctcaataaaa atcaaaaagc aaactagaaa gctaggcaca gactgctcaa
     2881 aacaccgttt t
//
LOCUS       DAVBCN010000121         2861 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVBCN010000121 DAVBCN010000000
VERSION     DAVBCN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.121"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(5..946)
                     /locus_tag="VQ497_001992"
     CDS             complement(5..946)
                     /locus_tag="VQ497_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET2518258.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(924..2687)
                     /gene="aspS"
                     /locus_tag="VQ497_001993"
     CDS             complement(924..2687)
                     /gene="aspS"
                     /locus_tag="VQ497_001993"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET2518259.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGTVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKEAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVFFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETVHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
BASE COUNT          830 a          678 c          569 g          784 t
ORIGIN      
        1 atcactaatt atctatctca acaaatcttc ctagaatatg aacattttcc gagacagaga
       61 caaaggagct tggatccact tgtgtcataa tctgtttaaa ttcattaaac tctgcacgtg
      121 taatgacagt gattaaaact gcctttctct cgtgattata ggttccttct gcatcgtgga
      181 tcatggttgc tccgcggtgc aattttttat ggattttttc aattaccttc tctggatgat
      241 ttgtcacaat catggcctgc atacgctttt gcttagtaaa gactgcgtct gtcacacggc
      301 tagagacaaa gatggtaatc atagaataaa gagcgtattt ccaaccaaag gtcaaacctg
      361 ctatcagcat gatagttcca tttaccaaga aagaaatact accgacattc ttacccgttt
      421 tcttacgaat agtcaggctg acgatatccg tcccaccact ggagatattg tttcgaagag
      481 caaaaccaat ccccaaaccc ataacaacac ccccaaaaag ggaattgata atgggatcct
      541 ccgtcaaggt tgccacaggg acaaactgga taaagaagga actcatagat accgtgataa
      601 aggtaaagac ggtgaactta tggccaatct gataccaagc taagaccatc aaagggaagt
      661 taatggcgta gaagcttagc gaaatcggaa tatgaaaacc aaaccagtga ttactcaagg
      721 cagagataat ctgtgccaga cctgttgcac cactcgaata cacatgccct ggttggaaaa
      781 agaaattaac tgctactgct gataaaaaac catagaccag agaggccgaa atcttctcat
      841 catacttttc tcgagagata ctttgtaaga cacgtaaaat ttttatctga taagcaaagc
      901 ggcgcagata atagcgccac cgcttaattc gttttgtttg tttcatcttc ttctacttgt
      961 aagctgagtt cctctagttg tttgagagcg actgttgatg gagcttgtgt cattgggtca
     1021 gttgccttgt tgttcttagg aaaggcaatg acttcacgga tattttcttc tccagcaagc
     1081 aacatgacaa aacggtcaag cccgatagcc aaaccaccgt gtggtgggaa accatagtcc
     1141 atggcttcaa gaaggaaacc aaactggtca ttggcttctt cagttgagaa accaagagcc
     1201 ttgaacatgc gttcttgaag gtctttttgg ttgatacgaa ggctaccacc accaagctca
     1261 taaccgttca agacgatatc gtaagcaatg gcacgaacct tagccaaatc accttctaat
     1321 tcgtgaacag tctcttcctg tggaagggtg aaaggatggt gggcgctcat gtagcggcct
     1381 tcttcttcag accattcaaa catcggccag tcaaccaccc aaaggaagtt gaacttatca
     1441 ttatcaatca agccaagctc tttagcaata cgtccacgaa gggcacccag tgttgcatta
     1501 gccacttcaa gcgtatccgc cacaaagaaa accaagtcct tatcttcaag agcaagcgct
     1561 gttgtcaatt cttcttggat accagtcaag aacttggcaa ctggtccgtt taattctcca
     1621 tcaaccacct tgacccaagc aagacctttg gcaccatact gtttggctac ttccgtcatc
     1681 ttgtcgatgt ctttacgtga atagttgtcc gcagcttctt tgaccacaat cgcttttaca
     1741 gcaggtgctt ctgaaaagac tttaaagtct acacctttga ccacttctgt caagtcctga
     1801 agcaacatgt caaaacgagt atctggcttg tcagaaccgt aaagagccat agcatcatcg
     1861 tatttcatac gagggaatgg tagcgttact tcgatgcctt ttgtttcctt catcacgcgc
     1921 gcgatcaagc cttctgtaat atcttggatt tcttgctcag taaggaagga cgtttccaag
     1981 tcgacctgag taaattcagg ctggcggtct ccacgcaagt cctcgtcacg gaaacattta
     2041 acgatttggt agtaacggtc aaaaccagca ttcatcaaga gctgtttcgt gatttgtgga
     2101 ctttgaggaa gagcgtaaaa atgcccctta ttaacacgag acggcactaa ataatcacgc
     2161 gccccttcag gcgttgactt agaaaggaat ggtgtctcca cgtcgataaa ctccaactca
     2221 tccaagtagt tgcggataga gtgggtcacc ttggcacgaa gtttaagatt ttccaacatt
     2281 tctggacgac gaaggtcaag gtaacggtaa cgcaaacgtg tatcgtcatt tgcctcaatg
     2341 ccatccttaa tctcaaatgg tgttgtctta gctgtgttaa gcacaataag agctgtcacg
     2401 tttaactcaa ccgtaccagt tggcaactta tcattggctt gctcacgcgc agcgacctga
     2461 ccagtcacct caataacaaa ttcgctacga aggctttcag ctgttgccat aacctctgca
     2521 gatacttttt cagggttgat aaccaactgc atgattcctt cacggtcacg aagatcgata
     2581 aagatcaaac caccaaggtc acgacgacgg ccaacccatc ctttcaaggt tatttcttgt
     2641 ccgatgtgtt cctcacgaac acgaccagca tacatactac gtttcattat ttctctcctc
     2701 ttttattctg ttactatttt accataaaag cgcaggctct tcatgaaaat catcagaaaa
     2761 gtttgccagt ctttaaaagt caggtgaaag ccctaaaaat tagcgctaat actcttcgaa
     2821 aatctcttca aaccacgtca gcgtcgcctt gccgtaggta t
//
LOCUS       DAVBCN010000122         2781 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVBCN010000122 DAVBCN010000000
VERSION     DAVBCN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.122"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            87..>2781
                     /locus_tag="VQ497_001994"
     CDS             87..>2781
                     /locus_tag="VQ497_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HET2518260.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSDHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFIFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEI"
BASE COUNT          856 a          451 c          609 g          865 t
ORIGIN      
        1 gcttgtgaga tagctataaa agtgtggaga aatagcttga aatttagtat aatatagcta
       61 aactatttgt ttaaagtgag aaaaaaatgg gaaattttag ctttctttta aaaaatgacg
      121 aatatgaatc tttttcaaaa ccttgcattg aagctgagaa tatgattgct acatcaactg
      181 tggctactgc ctttatggcg cgtcgtgctt tagagcaggc tgtccattgg atatatagtc
      241 acgattcata tttagaagct ccctatcgtg ctactctatc ttctttagta tgggatgatg
      301 attttaggga tatcgtagat tctgaactcc acaagcagat agttctgttg attcggtggg
      361 gaaaccatgc tgctcatggt ggtgaaatta aggaacgaga agcgatttta gctttgcatc
      421 atttgtatca gtttgttaat tttatcgatt attgttacag caatgagttt gtggagcgtt
      481 attttgatga gaagtgctta ccactttcag caaacatcaa ataccgagaa actccacaat
      541 ctatgataaa gttacaagac agtttaccag aactgcctga ttttcatgaa cagatggctg
      601 ctcagtccgt agaagttcaa gagacttata ctgaaaaacg tgagactgca gcgcaacggc
      661 aagatgtgcc tttccatatt gatcaattat ctgaggcaga gacaagaaag ctctttattg
      721 atatcgatct ccgtttagca ggatggatat ttgaagaaaa ctgtcgtgtt gagatagccg
      781 ttgatggtct caagcacggt tcaggaattg gttactgtga ctatgtactt tatggtaaaa
      841 atgggaaaat tttagcgatt gtggaggcta aaaaagcctc tgtcaatcca gaagtagggg
      901 aagtacaggt caaagaatat gctgaagctt tggagaaaca tatcggctat cagccaattt
      961 gctttattac aaatgggttg aagcactata tacttgatgg tccgaaccgc cgccagattg
     1021 caggctttta ctctcaagaa gaattgcaat tagtgatgga tagacgtcat cttcaaaaac
     1081 cgcttgagga tatttctagt aaaattaggg acgatatttc cgggcgtcac taccaaaaac
     1141 atgccattgc aagcgtttgt gaagctttct ctgatcatcg tagacaggca cttttggtta
     1201 tggcaactgg ggcggggaaa actcgtacag cagtttctct agttgatatc ttatcacgtc
     1261 ataactgggt aaaaaacgtt ctcttcttag ccgatagaac ttccttggtt aagcaagcat
     1321 atgattcgtt tagaaaatta ctcccagatc tttccgtttg taacttctta gaagataaag
     1381 aaggagctca atcaagtcga atggtctttt caacttatcc gaccatgatt ggagcgatta
     1441 gtggtcaaga agaagtaaat caacgccctt ttactgttgg gcattttgac cttatcataa
     1501 ttgacgaatc tcaccgttct atttatcaga aatacaagtc catttttgat tattttgatg
     1561 caaggattgt aggcttaaca gctactccgc gtcaagattt agataaaaac acctatggat
     1621 tctttaattt ggagaatggg gttccaacat atgcatatga tttggaagag gctgttaaag
     1681 acggatattt agtagcctat cattctatcg aaaccaaact gaaactacct acggatggtc
     1741 tacattatga tgatttgtcc gaagaagaaa aggaacattt tgatagcaaa tttgaagacg
     1801 atagctgtga aaaagatatt gatgggagtg tgtttaattc ctttattttc aataaaagta
     1861 cagtagaaat tgttttaaat gaactcatga caagaggaat tcagacagcc tcgggtgatg
     1921 aaattggtaa aactattatt tttgctaaaa atcatgacca tgcggaatat atcagaggta
     1981 tttttaacaa ccgctatcct gaaaaaggga gcgactatgc tcaggtgatt gattatagta
     2041 ttaagcatta tcagaccttg attgatgatt ttaaaattaa ggagaagtat cctcaaattg
     2101 cgatttctgt cgatatgtta gatacaggta ttgatgtacc agaggttgtt aatttagtct
     2161 tcttcaagaa agtacgctct aaaactaagt tttggcagat gattggtcga ggaacccgtc
     2221 tatgtaaaga tttatttgga cctgagcagg ataaggaaaa cttcttggta tttgattatg
     2281 gggacaattt tgattatttt cgtgcagatc caagagatgg agagggtcgt cacattgttt
     2341 cgctgactca gcgtttattt aatatcaaag tggacttgat tcgagaactt cagggactcc
     2401 aataccaaga agatcagttt gcgagagcat accgtcagca gcttgtctcg gaacttcaag
     2461 gtcgtataga gagcttaaat gagttggact tcagggttcg tatggtttta gatacagttt
     2521 atagctatag gaaattggaa agttggcaga atctaactgc tgttacaagt gaaaccattc
     2581 aaaaaaatct ctctccgctt ttatttgatg aagataaaga agatgagatg gcgaggagat
     2641 ttgatttgtg gttgcttcat attcagttgg ggcaactgac agctaaatct tccactgttc
     2701 atatttccca agtgatgaag acggctagag ctctttctgc tattggcaat atcccgcagg
     2761 tttttgagca ggctgaaatt a
//
LOCUS       DAVBCN010000123         2640 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVBCN010000123 DAVBCN010000000
VERSION     DAVBCN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.123"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(563..1432)
                     /locus_tag="VQ497_001995"
     CDS             complement(563..1432)
                     /locus_tag="VQ497_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET2518261.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(1655..2278)
                     /locus_tag="VQ497_001996"
     CDS             complement(1655..2278)
                     /locus_tag="VQ497_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2518262.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEKPIPQPNPEHPSVPTPNPELPNQETPTPDKPTPEPGTPKAETPVNPDPE
                     VPTYETGKREELPNTGTEANATLASAGIMTLLAGLGLGFFKKKEDEK"
     gene            complement(2236..>2640)
                     /locus_tag="VQ497_001997"
     CDS             complement(2236..>2640)
                     /locus_tag="VQ497_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HET2518263.1"
                     /translation="TFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANYYK
                     EGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPANA
                     YQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
BASE COUNT          735 a          551 c          508 g          846 t
ORIGIN      
        1 aacctcaaaa ctgtgttttg aacaacctgc ggctagcttc ttagtttgcc ctttgatttt
       61 cattgagtat aaaattgcag ataaaacgat aacaatcata ggactcagca gagtcaatgt
      121 aggtcgaaga atagcccccc taatcgctta ttttatagca aaatgaaata gaaactgaac
      181 attttgttga ggaaggtaaa tcaattcctc acaatgtttg agaaagaagg gaggactatt
      241 ccaacttcaa cccactataa agcagactta caagataggg aacccatctc cgataatata
      301 tagtagaatg aaaactgcac aatttgattg gagaattcaa accaatttct aacaatgttt
      361 tagaaacaag ggtctattat ttcaactgca acctactata gcccattgaa gctggaatag
      421 cccactgaga cttctaaacc actgctggag aggagtttgt ttagagtttc tttcatttcc
      481 ctaaaaaatc aaaaagagag aaccagtctg atcctctctt acttatagat ggtgctcact
      541 caaatgttaa aggagctatt tcttagagat aaaggaattt gaatacagct actgccgcaa
      601 ttgctgctgc gataggtgct actactggta cccaagaata ccaccatttt gaatcgcctt
      661 tatgctcacc aagaactgat tttggaagga aagcatgaag gagacgtggt cccaagtcac
      721 gggctgggtt caaggcaggt cctgtaggtc ctccaagtga tgttaccaaa gccataacga
      781 ggaatccaag tgccaagtga gccacagaaa gtcctgaagc agtgtgtgga gccacctgtg
      841 ctttaatagc caagtcagaa aaatcaactg tttgtcctgc ttctattgcc ttttgtttca
      901 tgaattgaag cacttcagca ccaaagaagt ttttagtcaa accaagagct gcaaagaaca
      961 aaacaaatga accaacaaac tcattaatca aaccattgac agttgctgca tagcgacttt
     1021 cttttgtacc atggtcaata cttgaaatag ttgagaaagt tcccaagatg ttatttggtt
     1081 tttcagtttt caagtagaat ggacggtatg ttgccacaac taaggcttgg ccaaagatag
     1141 cccccaagac ttgcgcgata atgtaaggta ccacttgtgc ccaagggaaa agaccgctaa
     1201 ctgcaagccc tagagtgaaa gcagggttga tgtgattccc agatacgtta ccaaacatca
     1261 aggctgggat cataaccccc ataccataac caacagcgat gacgatccag ccactttggt
     1321 gacctttcgt acctttaagt tcaacgttgg caactgcacc attcccaaga atgatcaaaa
     1381 tggcagttcc caaaaattca gtggcatact tcagtgccca tgtgaaatcc atttgctaga
     1441 ttctccttaa aattttttag atattcctta ttttatcaat ttttacccta tttagcaact
     1501 gatttctgga aatattttta aaaaaatagg attcatagtt tcccagttca acgtacttgt
     1561 aagcgttttt attcttcacg aaagacctca ttctcaaaac caggaaaaag cccacatctg
     1621 tgatcttttc ctggttccta gattctaaaa tctattattt ttcatcttct tttttcttga
     1681 agaatcctaa tcctagacca gctaacaagg tcatgatacc agcactagcc aaggtagcat
     1741 tagcttctgt acctgtgttt ggcaattcct ctctcttacc tgtctcataa gtcggaactt
     1801 ctgggtctgg attcactgga gtttcagctt ttggagtacc tggttctgga gttggtttat
     1861 ctggtgttgg agtctcttga tttggtagtt ctgggtttgg tgtcggaaca cttggatgct
     1921 ctggatttgg ttgtgggatt ggtttttctg gagttggttt atctggtgtt gataaacggt
     1981 cataccttac cgttatttct ttatcactag agtctgacgt aacttcttgt gattcaactg
     2041 ttggaatatc tggatctttg tacttgtcaa tcttaccaga tataacctcg tcccagtttc
     2101 ctgttgtcca ttcaccgtag gttacaactc ccgtgacctt gttctcagtt tttgtacggc
     2161 ttaaggttac aggttgaaca acatcttctt ttacattttg gttcgtaact ttatcaacgt
     2221 aatgaatgat acgcgttata gtcttcgtct cagtagaggt tgctgttttg ggaaccactg
     2281 tttcctcaac attctcacgg tagtaatagt caactgttgc accgtcttct ggtacgcatt
     2341 tgcaggagtt gctaccaagg tgtatgttgt tttccttgtg ataactcggt cttctttgtc
     2401 ctcagttgtt gttttccctt caatagtttt tgattctgtg gtatactcag aacctatcgc
     2461 taaatcagct tttataacag actctgccaa cttctcttgg ctaccttctt tatagtaatt
     2521 ggctgttact gtagcagtgg ttggcgcttc gctttctcta taaactaagg tcactgttct
     2581 accttcgctt acaatattcc cagttaaact tgcagaattt gtatctgctt ctttaaaagt
//
LOCUS       DAVBCN010000124         2607 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVBCN010000124 DAVBCN010000000
VERSION     DAVBCN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.124"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            223..537
                     /locus_tag="VQ497_001998"
     CDS             223..537
                     /locus_tag="VQ497_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518264.1"
                     /translation="MWVLVFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            547..753
                     /locus_tag="VQ497_001999"
     CDS             547..753
                     /locus_tag="VQ497_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2518265.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            753..950
                     /locus_tag="VQ497_002000"
     CDS             753..950
                     /locus_tag="VQ497_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518266.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            953..1675
                     /locus_tag="VQ497_002001"
     CDS             953..1675
                     /locus_tag="VQ497_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HET2518267.1"
                     /translation="MKKKRRLLFLMSIVLGMFVGMFKARVESHEIILDVKALMPWISA
                     ICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAFNITG
                     VAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLEFFAT
                     PKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQLLAFL
                     LVGAIHVYINVMQLPMVKRYFK"
     gene            1687..2328
                     /locus_tag="VQ497_002002"
     CDS             1687..2328
                     /locus_tag="VQ497_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2518268.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPVLVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            2346..2576
                     /locus_tag="VQ497_002003"
     CDS             2346..2576
                     /locus_tag="VQ497_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518269.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
BASE COUNT          765 a          432 c          530 g          880 t
ORIGIN      
        1 cgaagtcagt aaccatacct acggcgaggt gaagctgacg tggtttgaag agattttcga
       61 agagtattaa tgatagataa actggtcagt aacctactcc tgaccttttt cttttgcaaa
      121 atgacaaaaa tcataaactt tttgacaact atacttgtca aaaagaaaaa gatgtgttac
      181 aatgaattca agttaagaaa taggaagcag aaaggagtta taatgtgggt actagtattt
      241 atactattta tgattttctt ttattctaat aattctaaaa aaatcaagaa actagagaat
      301 aaaatcaaaa aacttgagcg aaaagagaaa ggaaacgcag aaatgtcgag attattgcaa
      361 gaaatgattg gaaaggaacc aattataacg ggagtgtata ttgggccaga taactgggaa
      421 gttgtggatg ttgatgagga atgggtaaag ctacgacgtg tagataatac gggaaaagaa
      481 aaattcaagt tgcaacgcat tgaggatatc caaaccgttg aatttgacgg agagtaggtg
      541 tgtaacatgc aacttaaaaa tcgtctaaaa gagcttagag ctcgcgatgg tctcaatcaa
      601 accgacctag ccaaactggc aggggtttcc agacagacca ttagcctact agaacgggat
      661 gagtacaccc catccattat cattgccctg aaaatatctc aaattttcaa cgaaacagtc
      721 gaatcggtat ttcgtttgga ggaggacgag tgatgaacaa gtataaagtg atttattatg
      781 tagtggtcat agctttatta gtcagtgtat ttctactgat tgggatggac ctaagctggt
      841 ttaatcccta tcaaagcgac caatttgttt gggtctactt tgctctcatc ccagtaattg
      901 aatggattga aaagaaatcc aaaaatctag caagtgaaaa aggagaatga atatgaaaaa
      961 gaaacgtaga ttgttatttt tgatgtctat tgtcttgggc atgtttgtag ggatgtttaa
     1021 ggcacgtgtc gaatcccacg aaattatttt agatgtaaaa gccttgatgc catggatatc
     1081 agctatttgt ttactgatag gtttcattag tatgtttttg actttcaatt tcttaaagaa
     1141 aagcagaaaa tttcattcct tgtatcaaga ggaaatggat gacgatctga atgaaaccta
     1201 ttatgtgcaa atgtatcgga atcttgagtt tggaaccatt gcttttaata ttacaggtgt
     1261 agcgattcca ttggctattt ttatttcatt aagtgaggtg attatattgc atacaaaccc
     1321 tcaaacattt ttcctttctt tcttactctt tgtggtattc ttagtcgctc aaaaatctct
     1381 ttttaaaacc attgcgattg ttcgtcagtt tgatttagaa tttttcgcta caccaaagga
     1441 tgtcttgaac tatataaatt cttatgatga aggggagcgt caggctaatt tggaacagag
     1501 ttttcgaatt ttattccaat tacaccaata tgtcttacca gccttatata tttttcttat
     1561 tatcatttct ttcttgacag gagagattca gttactagct ttcttgcttg taggagccat
     1621 ccatgtttat atcaatgtga tgcagttacc tatggtaaaa cgttatttca aataaaggag
     1681 ttatctatga aattacttaa aaaccttggc tggattcttc tagcccttct atccttttta
     1741 tttatctatg gctttattca ggggctcgcg actgcgtcgc ttgctttagg cgcttcaccc
     1801 tatgctgtta ctctactcta tgtggccttg gctggagtat atgtgtacgg catttacaaa
     1861 tggtatcaga aggctcctgt tcatattgag aagagcggct ttaatcgatt tatttggctt
     1921 cctgtcttgg tttggttctt atctctggtc gttcaattct tcttgccaga tgatccttca
     1981 gtaaatcagc aaatagcgac agacttgacc ttgtctcaac cacttttctc attctttgct
     2041 gtggttattt ttgctccttt gacggaagag attgttttta gagggatgtt agcacgctat
     2101 ctctttccta agcaggacaa tagtaaacga accctgattt ttcttctggt atctagtctt
     2161 ctatttgcct tgattcattt tccaggtgat gtgcaacaat tttttgtcta ttttagcctt
     2221 ggttttagtt tgggtttggc ttacattagc agaaaaggtc tggtctacag tatttctctt
     2281 cacgctttga ataatttagt cggctttttg atgattctca tgctataata gagtcaggag
     2341 gtcacatgaa acgagtaatt ttattagcag tgattcaggc agtcgttcta tttttcatca
     2401 ttggagcgct agcttatgcc ttcaaaggcg atttcttcta taactatcta gcagttgtct
     2461 ttgctcctat tgcaggtgta ctgcgttttg ggacggctta cataacggaa attgtcttgc
     2521 ctcgaaaggc agccgaaatc gctgaaaagc gtaaagcagg caaaaattca aaataaaaga
     2581 gtccggtgaa gttcatcgga tttttgt
//
LOCUS       DAVBCN010000125         2545 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVBCN010000125 DAVBCN010000000
VERSION     DAVBCN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.125"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            352..2457
                     /locus_tag="VQ497_002004"
     CDS             352..2457
                     /locus_tag="VQ497_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2518270.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTSEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSQLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMKDGVLVIREKA"
BASE COUNT          821 a          463 c          589 g          672 t
ORIGIN      
        1 ttgtagataa ggagaagctg atgtggtttg aagaggtttt cgaagagtgt aaaaaaatat
       61 tggtcagtta aagccaaaac tcttgacctt ttctgaccaa tgtagtatat tatgaacgta
      121 aggtaaatga aagccttact agaacactta tttaaagtaa aatagaaaga ggtgggtcta
      181 tgtttcatct agaaatcttc agaagtaaag atggtctact cctgcttgaa aaagaaaaac
      241 cagaaatagt acgtagggta gcggtttaac tagtctaaat tttttaaaac aatggtcaaa
      301 gatagtcaat atcagtaatc ataactaagt aaacaaaaag aggtaaagaa tatgaataac
      361 aactttaata attttaataa catggatgat ttatttaacc aattgatggg tggtatgcga
      421 ggatatagtt ctgaaaatcg ccgttactta attaatggac gcgaagtcac atctgaggaa
      481 tttgctcact atcgtgcgac tggtcaatta ccaggaaatg cagaaactga tgtgcaaatg
      541 ccacaacagg catcaggtat gaaacaagac ggtgtccttg caaaactagg tcgaaacttg
      601 acagcagaag cgcgtgaggg caagttggat cctgttatcg gacgaaacaa ggaaattcaa
      661 gaaacatctg aaatcctctc acgccgcacc aagaacaatc ctgttttggt cggagatgca
      721 ggtgttggta agacagcagt tgtcgaaggt ctagcgcaag ccattgtgaa cggagatgtt
      781 cctgctgcta tcaagaacaa ggaaattatt tctattgata tctcaggtct tgaggctggt
      841 actcaatacc gtggtagctt tgaagaaaat gtccaaaact tagtcaatga agtgaaagaa
      901 gcagggaata ttatcctctt ctttgatgaa attcaccaaa ttcttggtgc tggtagcact
      961 ggtggagaca gtggttctaa aggacttgcg gatattctca agccagctct ctctcgtgga
     1021 gaattgacag tgattggggc aacaactcaa gacgaatacc gtaacaccat cttgaagaat
     1081 gctgctcttg ctcgtcgttt caacgaagtg aaggtcaatg ctccttcagc agagaatact
     1141 tttaaaattc ttcaaggcat tcgtgacctc tatcaacaac atcacaatgt catcttgcca
     1201 gacgaagtct tgaaagcagc ggtggattat tctgttcaat acattcctca acgtagcttg
     1261 ccagataagg ctattgacct tgtcgatgta acagctgctc acttggcggc tcaacatcca
     1321 gtaacagatg tgcatgctgt tgaacgagaa atcgaaacgg aaaaagacaa gcaagaaaaa
     1381 gcagttgaag cagaagattt tgaagcagct ctaaactata aaacacgcat tgcagaattg
     1441 gaaaagaaaa tcgaaaacca cacagaagat atgaaagtga ctgcaagtgt caacgatgtg
     1501 gctgaatctg tggaacgaat gacaggtatc ccagtatcgc aaatgggagc ttcagatatc
     1561 gaacgtttga aagatatggc tcatcgcttg caagacaagg tgattggtca agataaggcc
     1621 gtagaagctg tagctcgtgc tatccgtcgt aaccgtgctg gttttgatga aggaaatcgc
     1681 ccaatcggca gcttcctctt tgtagggtct actggggttg gtaagacgga gcttgctaag
     1741 caattggcac tcgatatgtt tggaacccag gatgcgatta tccgtttgga tatgtctgaa
     1801 tacagtgacc gcacagctgt ttctaagcta attggtacaa cagcaggcta tgtgggttat
     1861 gatgacaata gcaatacctt aacagaacgt gttcgtcgca atccatactc tatcattctc
     1921 ttggatgaaa ttgaaaaggc tgatcctcaa gttattaccc ttctcctcca agttctagat
     1981 gatggtcgtt tgacagatgg tcaaggaaat acagtaaact tcaagaacac tgtcattatt
     2041 gcgacctcaa atgctggatt tggctatgaa gccaacttga cagaagatgc ggataaacca
     2101 gaattgatgg accgtttgaa acccttcttc cgtccagaat tcctcaaccg ctttaatgca
     2161 gtcatcgagt tctcacaatt gactaaggaa gacctttcta agattgtaga tttgatgttg
     2221 gctgaagtta accaaacctt ggctaagaaa gacattgact tggtagtcag tcaagcggct
     2281 aaagattata tcacagaaga aggttacgac gaagtcatgg gggttcgtcc tctccgtcgc
     2341 gtggttgaac aagaaattcg tgataaggtg acagacttcc acttggatca tttagatgct
     2401 aaacatctgg aagcagatat gaaagatggc gttttggtta ttcgtgagaa agcctaagac
     2461 agaattttga ggataaaaaa gaaggagcca gctgaaaaaa ctggttcctt ttgaggtacg
     2521 acgggcatgt cgtatagtag aagtg
//
LOCUS       DAVBCN010000126         2508 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVBCN010000126 DAVBCN010000000
VERSION     DAVBCN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.126"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..2253
                     /gene="phtA"
                     /locus_tag="VQ497_002005"
     CDS             <1..2253
                     /gene="phtA"
                     /locus_tag="VQ497_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HET2518271.1"
                     /translation="EQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEELLMKDPNY
                     KLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHSQHREGGTP
                     RNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNELSASELA
                     AAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSNTNSQASQS
                     NDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYHFIPYSQMSE
                     LEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSNSSLVSQLV
                     RKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKENVAPRDQE
                     FYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDLLAFLAPIT
                     HPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYVTPHMGHSH
                     WIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVKGEKRIPLV
                     RLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLFATIKYYVE
                     HPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVPQVETEKVE
                     AQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKLLALLKGSN
                     PSSVSKEKIN"
     gene            2411..>2508
                     /locus_tag="VQ497_002006"
     CDS             2411..>2508
                     /locus_tag="VQ497_002006"
                     /inference="COORDINATES: protein motif:HMM:TIGR01363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2518272.1"
                     /translation="MKINKKYLAGSVATLVLSVCAYELGLHQAQTV"
BASE COUNT          882 a          467 c          496 g          663 t
ORIGIN      
        1 gagcaaatcg tcatcaagat aacagaccaa ggctatgtca cttcacatgg cgaccactat
       61 cattattaca atggtaaggt tccttatgat gcgattttca gtgaagaatt actcatgaaa
      121 gatccaaact ataagctaaa agatgaggat attgttaatg aggtcaaggg tggatatgtt
      181 atcaaggtag atggaaaata ctatgtttac cttaaggatg ctgcccacgc ggataacgtc
      241 cgtacaaaag aggaaatcaa tcgacaaaaa caagaacata gtcaacatcg tgaaggtggg
      301 actccaagaa acgatggtgc tgttgccttg gcacgttcgc aaggacgcta tactacagat
      361 gatggttata tctttaatgc ttctgatatc atagaggata ctggtgatgc ttatatcgtt
      421 cctcatggag atcattacca ttacattcct aagaatgagt tatcagctag cgagttggct
      481 gctgcagaag ccttcctatc tggtcgggga aatctgtcaa attcaagaac ctatcgccga
      541 caaaatagcg ataacacttc aagaacaaac tgggtacctt ctgtaagcaa tccaggaact
      601 acaaatacta acacaagcaa caacagcaac actaacagtc aagcaagtca aagtaatgac
      661 attgatagtc tcttgaaaca gctctacaaa ctgcctttga gtcaacgaca tgtagaatct
      721 gatggccttg ttttcgaccc agcgcaaatc acaagtcgaa cagctagagg tgttgcagtg
      781 ccacacggag atcattacca cttcatccct tactctcaaa tgtctgaatt ggaagaacga
      841 atcgctcgta ttattcccct tcgttatcgt tcaaaccatt gggtaccaga ttcaaggcca
      901 gaacaaccaa gtccacaacc gactccggaa cctagtccag gcccgcaacc tgcaccaaat
      961 cttaaaatag actcaaattc ttctttggtt agtcagctgg tacgaaaagt tggggaagga
     1021 tatgtattcg aagaaaaggg catctctcgt tatgtctttg cgaaagattt accatctgaa
     1081 actgttaaaa atcttgaaag taagttatca aaacaagaga gtgtttcaca cactttaact
     1141 gctaaaaaag aaaatgttgc tcctcgtgac caagaatttt atgataaagc atataatctg
     1201 ttaactgagg ctcataaagc cttgtttgaa aataagggtc gtaattctga tttccaagcc
     1261 ttagacaaat tattagaacg cttgaatgat gaatcgacta ataaagaaaa attggtagat
     1321 gatttattgg cattcctagc accaattacc catccagagc gacttggcaa gccaaattct
     1381 caaattgagt atactgaaga cgaagttcgt attgctcaat tagctgataa gtatacaacg
     1441 tcagatggtt acatttttga tgaacatgat ataatcagtg atgaaggaga tgcatatgta
     1501 acgcctcata tgggccatag tcactggatt ggaaaagata gcctttctga taaggaaaaa
     1561 gttgcagctc aagcctatac taaagaaaaa ggtatcctac ctccatctcc agacgcagat
     1621 gttaaagcaa atccaactgg agatagtgca gcagctattt acaatcgtgt gaaaggggaa
     1681 aaacgaattc cactcgttcg acttccatat atggttgagc atacagttga ggttaaaaac
     1741 ggtaatttga ttattcctca taaggatcat taccataata ttaaatttgc ttggtttgat
     1801 gatcacacat acaaagctcc aaatggctat accttggaag atttgtttgc gacgattaag
     1861 tactacgtag aacaccctga cgaacgtcca cattctaatg atggatgggg caatgccagt
     1921 gagcatgtgt taggcaagaa agaccacagt gaagatccaa ataagaactt caaagcggat
     1981 gaagagccag tagaggaaac acctgctgag ccagaagtcc ctcaagtaga gactgaaaaa
     2041 gtagaagccc aactcaaaga agcagaagtt ttgcttgcga aagtaacgga ttctagtctg
     2101 aaagccaatg caacagaaac tctagctggt ttacgaaata atttgactct tcaaattatg
     2161 gataacaata gtatcatggc agaagcagaa aaattacttg cgttgttaaa aggaagtaat
     2221 ccttcatctg taagtaagga aaaaataaac taatgaaaaa tgaaagtctc gataaagagg
     2281 ctttcatttt tattatgtat atatgtaaaa ttcttgacaa gcaatattaa aaagagtaaa
     2341 ctattaacta gttaattaac cggtttatta ctttatagtg aatcaaatat acttaagaaa
     2401 agaggaaaga atgaaaatca ataaaaaata tctagctggg tcagtagcta cacttgtttt
     2461 aagtgtctgt gcttatgaac taggtttgca tcaagctcaa actgtaaa
//
LOCUS       DAVBCN010000127         2324 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVBCN010000127 DAVBCN010000000
VERSION     DAVBCN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.127"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(7..450)
                     /locus_tag="VQ497_002007"
     CDS             complement(7..450)
                     /locus_tag="VQ497_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HET2518273.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            554..1489
                     /locus_tag="VQ497_002008"
     CDS             554..1489
                     /locus_tag="VQ497_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET2518274.1"
                     /translation="MEICQQILEKIKEYNTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNVTDAETAAIVGAPGRIDSVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            1543..1827
                     /locus_tag="VQ497_002009"
     CDS             1543..1827
                     /locus_tag="VQ497_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HET2518275.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(1884..2102)
                     /locus_tag="VQ497_002010"
     CDS             complement(1884..2102)
                     /locus_tag="VQ497_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518276.1"
                     /translation="MSISPRFEALEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     TAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(2099..2324)
                     /locus_tag="VQ497_002011"
                     /pseudo
     CDS             complement(2099..2324)
                     /locus_tag="VQ497_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          689 a          521 c          437 g          677 t
ORIGIN      
        1 ttatgcttat cctactttag cagccaattc ttctgcgaat tgttccaagc gttcaatatc
       61 ttcttcctcg gcagaaagat caactttaac acactctgaa cctttttctg ctcctgttga
      121 cacaaagaca cggtcaaagt catcaacagc cttacagaat tcatcgtaga aggtatctcc
      181 tgagccgacc actccgtaga ttttgccatt caagttgaga tctgctaggt cttcgtagaa
      241 gtccatcatc tcatctggca attcaccatc accataagta taggtcgcaa cgatagcgat
      301 gtctgcttcc aagaagtctg aagcgtcaac agttgtacat tcatcaacat caacatccaa
      361 gcccaagtca cgtaatttgt ctgctacaat atctgcaatt tcttcggtat taccggtcat
      421 actggcaaat acaatttttg ctaatgccat atcgtcctcc tcaatttatc tttctccatt
      481 atatcacatc tttttcattt taaaaataaa aattcttgtg ctacaatgaa atgagaaaga
      541 attgaggtta tttatggaga tttgccaaca aattttagaa aaaattaaag aatacaacac
      601 cattatcatt catcgtcata tgaaaccaga ccctgatgcc ttgggaagtc aggtgggatt
      661 gaaagccttg ctggaacatc atttcccaga aaaaaccatc aaagccgtcg gttttgatga
      721 accaactctt acttggatgg ctgagatgga tcttgttgaa gatagagcct accaaggcgc
      781 acttgtcatc gtctgtgata cagctaatac tgctcgtatc gatgataagc gctatagtca
      841 aggtgatttt ctcattaaga ttgaccacca tccaaatgat gatgtatacg gtgacctgtc
      901 ttgggtcgat actagttcaa gtagcgctag cgagatgatt accctatttg cccaaacaac
      961 ccaactagcc ttggcagatc gcgatgctga gttgctcttt gcaggaattg tcggtgatac
     1021 aggtcgcttc ctctaccctt ctaccactgc acggactctt cgcctggctg cttatttgag
     1081 agaacataac tttgactttg cggctctcac tcgcaaaatg gacactatga gctacaaaat
     1141 tgctaaactg caaggctaca tctacgacca tctggaagtg gatgaaaatg gtgctgctcg
     1201 cgttatcctg agtcagaaaa tcttgaaaca atacaatgta accgatgctg aaactgcggc
     1261 cattgtaggt gcacctgggc gtattgatag tgttagtctc tggggaattt ttgtggaaca
     1321 ggctgatggc cactaccgcg ttcgcttacg cagtaaaatc catcctatca atgaaatcgc
     1381 caaggagcat gatggtggag gccaccctct agcaagtggt gctaattcct atagcctaga
     1441 agaaaatgaa atcatctacc aaaagttaaa aaacttgctt aaaaactgat aaaatacttg
     1501 ccaaactttt cagaatctga tagactagta tggtaacaat ctatggctcg caaagagacc
     1561 atggcagaaa ggaaatattg caaaatgaaa aaagatatcc atccagaata tcgcccagtt
     1621 gtcttcatgg acacaactac tggttaccaa ttccttagcg gttcaacaaa acgctctaac
     1681 gaaacagttg agttcgaagg cgaaacttac ccattgatcc gtgtggaaat ttcatcagac
     1741 tcacacccat tctacactgg acgtcaaaag ttcactcaag cagatggacg cgtggatcgt
     1801 ttcaacaaaa aatacggtct caaataatga taaaagaaca gtttcgactg ttcttttttg
     1861 tttcttgaaa tcaactgctg ttttcatgtt ccagactcat ctgtaggttc ggtttccatg
     1921 ctactaggca ggaaggaaat agctgtttca acacgtccat aatgagctgt actattgtca
     1981 cgaaccacac tttcattgat ggtccaagtg gaattcattt tcttaaaagc ttctcggact
     2041 ttttccaaat ctttggaggc aatggcctgt tccaaggctt caaaacgagg acttatactc
     2101 atctgctttc aaaaagcatt ctagtccatc tccgattaac gatggacttt atcacctcct
     2161 tctccagtcc ttgtataaca tcttggagtt gattcatgac atcttccaaa gtttaaaagg
     2221 ctttattctt aaatccacgt ttacgaatct ctttccacac ttgttcaatg ggttcatctc
     2281 tggtgtgtat ggaggaataa atgcaaagcc aatattagtc ggaa
//
LOCUS       DAVBCN010000128         2215 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVBCN010000128 DAVBCN010000000
VERSION     DAVBCN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.128"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            101..1078
                     /locus_tag="VQ497_002012"
     CDS             101..1078
                     /locus_tag="VQ497_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2518277.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYKTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            1075..2157
                     /locus_tag="VQ497_002013"
     CDS             1075..2157
                     /locus_tag="VQ497_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2518278.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILTVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
BASE COUNT          700 a          500 c          402 g          613 t
ORIGIN      
        1 aaagctaata gcttatttga aattttctcc tctctgttgg atagtcccgt ctatctatgt
       61 tataatgaaa gaaggattta aaatcggaaa aggagtattt atgctaaaat taggtgtcat
      121 cggaactggc gctatcagcc atcatttcat agaagcagcc cataccagtg gagaatacca
      181 actggtcgca atctattcta gaaaactaga aactgcagca acctttgctt ctcgctatca
      241 gaatatccaa ctattcgatc aattagaagt cttcttcaaa tcttcttttg atttagtcta
      301 tatcgccagt ccaaactcct tacattttgc tcaggcaaaa gctgccttgt ctgctggtaa
      361 acatgttatt ctcgaaaagc cagctgtcac tcaaccacaa gaatggtttg atttgattca
      421 aacagctgaa aaaaataact gttttatctt tgaagcagct cgtaattacc acgagaaagc
      481 ctttactact atcaaaaact ttttagcaga taagcaagtg ttaggagcag atttcaacta
      541 tgccaaatac tcttccaaga tgcctgactt gttggctgga caaacaccaa atgtcttttc
      601 agaccgtttt gctggtggag ccctcatgga tttggggatt tatcccctct atgctgctgt
      661 tcgtcttttt gaaaaagcta atgacgcgac ttaccatgct caacagcttg acaatagcat
      721 tgacctaaat ggagatggta tcctcttcta cccagactat caagttcaca tcaaggctgg
      781 aaaaaacatc acttccaatc ttccttgcga gatttataca acagatggaa ccttgactct
      841 caacacgatt gagcatattc gctcagctat ttttaccgac caccaaggaa atcaagtcca
      901 gcttcctatc caacaggctc ctcatacgat gactgaggaa gtcgctgcat ttgcacacat
      961 gatccagcaa ccagatctga atctctacaa aacttggctg tatgatgcag gctctgttca
     1021 tgagctacta tataccatgc gccagactgc tggcattaga tttgaggcag aaaaatgaaa
     1081 accaaactac cgactgaatg gcaagaactg agtgaccaac tcggtttcca agaattcacc
     1141 cccattcaaa ctcaactatt tgagcccctt cttgctggag aaaacctcct aggagtgagc
     1201 caaaccggaa ctggtaagac cctagcgtat ctcctaccaa gtcttctcag actacaaaag
     1261 aaaaaagccc aacaactctt gattctagca ccaaacacgg aacttgctgg acagattttt
     1321 gatgtatgta aaacgtgggc agaagctatc ggattgactg ctcaactctt cctttcaggt
     1381 tcaagtcaga aacgacaaat tgaacgtctt aaaaaggggc cagaaattct gattggaact
     1441 cctggccgta tctttgaact cattaaactt aaaaaaatca agatgatgaa tgtggaaacc
     1501 atcatcctgg atgaatttga ccaattacta gatgattctc agattcactt tgtagaaaaa
     1561 atcactcact acgcacctcg tgaccaccaa ctggtctaca tgagtgccac gaccaagttt
     1621 gaccaagaaa agattgtgcc gaatacacgc actattgatc tctctgacca aaaattggac
     1681 aatatccagc atttctacat gcaggtagac caacgtcatc gagtggatat gctacgaaaa
     1741 ctggctcatg tagaggattt ccgtggtcta gtcttcttca atagcctgtc agaccttggg
     1801 aatgccgagg aaaaactaca gtatcgtgat atattgactg tttccctcgc tagtgatgtt
     1861 aatgttaaat ttagaaaaat catcttagaa aagtttaaag ataaccaact aaccctgctt
     1921 cttgcaactg accttttggc tcgtggaatt gatatcgata gcctagaatg tgtcgtaaac
     1981 tttgatattc ctagagatag cgaaacctac acacaccgtg ctggccgtac tggccgtatg
     2041 ggcaaggaag gatatgttat tactctcgtc actcatccag aagaaatcaa aaaacttaaa
     2101 aagtttgcaa gtatccgaga aattgtccta aaaaaccaag aactctatat caaataaagc
     2161 cccattgctc tatacacatc cagtgtatgg agctttttta taagcaattc aattt
//
LOCUS       DAVBCN010000129         2207 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVBCN010000129 DAVBCN010000000
VERSION     DAVBCN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.129"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(<1..1475)
                     /locus_tag="VQ497_002014"
     CDS             complement(<1..1475)
                     /locus_tag="VQ497_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET2518279.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT          655 a          488 c          402 g          662 t
ORIGIN      
        1 tattgacttt tcttatagtt tgtctttctc ttaataacgt tgatatgatt gacctctgga
       61 tcttccgtcc cttggaggga gcaaccacgt tccttggcga attggatata gtcgacaatt
      121 tctcgtgtaa tgcgttcacc aggagccaag ataggaatac ctggagggta acacataaca
      181 aattctccac agacctgtcc aacagaatca tccaaagtta aactttttct ttctgaatag
      241 aaggcttctt gcggagacag cactaactcg ggctgaatat attctcctgc tatcaaatct
      301 tttccatctc ttgaatagag tctcttaata tcagccagag caccaaccaa gcgctcgatg
      361 tcttggatgc ggtcgccgat ggaaatatag gccaagatat tgccgatatc accaaactcg
      421 atctgaatgt cgtattcgtc tcgcaagagg tcataaacct cgatacctgt taagccaata
      481 ccctgagtgt aaactgacaa cttagttacg tcaaaatcgc aaaccgaaac accgtctatt
      541 aactcttttg agtaggcata gtagccaccg atagcattga tttcatgacg agcgtactcg
      601 gatagctcaa tgacttcctc aaacgactct ttaccacgaa gggccaagtt gcgacgtgaa
      661 atatccaaac tagccatcaa taagtaagag gcagatgtag actgggtcag gtttataatc
      721 tgacgaacgt actcgggatt catctgctcc ccgattaaaa gaagcgaact ttgtgtcaaa
      781 ctcccaccag acttatgcat ggaaactgct gccatatcag ctcctgcatc catagcagaa
      841 attggaagtt taccagtgaa atgcaaatgc gctccatggg cttcatctac taaaaccatc
      901 atgccagctt catgagccat ttctgtcaac ccctttaggt ctgaacagat gccgtagtaa
      961 gtaggattgt tgattaggat agccttagca tctggatggt cctttatggc ctgtgctact
     1021 cggtcatttt caagacctaa agcgatacca atcttaggat ctacactcat ctcgatatag
     1081 atgggaatgg caccacatag aaccaacgca ttgatagcag atttatggac atttcgtggc
     1141 agaataatct tatctcctgc cttgcaggtt gccagaatca tagtctgcac cgatgaagtt
     1201 gttccaccaa tcattagaaa ggcatggcta gctccaaaag catctgcagc cagctcctct
     1261 gcatcacgaa taatcgaaat aggatggcct aaattatcca agggtttcat cgaattgaca
     1321 tcaatgccta cacatttttc tcctaagagt tcgacaagtt ctggatttcc ccgtccacgc
     1381 ttgtgacctg gaacatcaaa gggaacaatc cttttcttgc gtaacttcac caaggcctca
     1441 taaattgggg cttggttttg atctaactct ttcaaaacat actcctttca atatttttag
     1501 gaccttatga aaactaagga actaaaggtt gactcatgaa aaaagagcag gttttcacct
     1561 gctctgcaat cttctgacgt gtttattttg gtttctgttg agtatgtctg ttcccgatgt
     1621 ttcctaactc tctagtagca aatattacgt actttattga tcgtttcaca agatgacgtg
     1681 tgctttcacc acactaaaaa tttatgaatt aggacaagtg tcctaacatc aacttataaa
     1741 agtaggcttt taaccctatc aataatgtgg cttttcaacc acgcttcggc attcaaccct
     1801 gcctgtccgt aggcattttt tactcgggtt tatatttgct agaaaacatc atctcatttt
     1861 ctaagcttta ttactatatc atgtttttaa gaacttgtaa agtatttttt gataaaaaac
     1921 aaaaatataa tagattgaaa ctagaatagt acacctctgc ttctaaaaca ttgttagaaa
     1981 tagattttac tgtcctgatc gatttgttct gttcttgttt cattttacta tatgttcgta
     2041 aatgaaagtt ctataatgaa gttagccacc cagccctcct aaaaattaga tggttcaact
     2101 agctgatagc tcttgtaggc ataagaaaac acctctgtgt tatacttgtt gttcaaccac
     2161 aaacacaaga aaggcacaga aatgcaagac aattatacta caaaagg
//
LOCUS       DAVBCN010000130         2154 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVBCN010000130 DAVBCN010000000
VERSION     DAVBCN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.130"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            85..534
                     /locus_tag="VQ497_002015"
     CDS             85..534
                     /locus_tag="VQ497_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2518280.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDAFVTNFVYEKEGQSRKKSMSYESVLPVRQIFD"
     gene            691..1101
                     /locus_tag="VQ497_002016"
     CDS             691..1101
                     /locus_tag="VQ497_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HET2518281.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKRIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            1217..>2154
                     /gene="pavB"
                     /locus_tag="VQ497_002017"
     CDS             1217..>2154
                     /gene="pavB"
                     /locus_tag="VQ497_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HET2518282.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVKDNVKDS"
BASE COUNT          662 a          411 c          477 g          604 t
ORIGIN      
        1 tctatacggc aaggcgacgt tgacgcggtt tgaagagatt ttcgaagagt ataagaaaaa
       61 atcagtcccc taaaggagca gattatgaag ttattgtcta tcgccatttc tagctataat
      121 gcagcagcct atcttcatta ctgtgtggag tcgctagtga ttggtggtga gcaagttggg
      181 attttgatta tcaatgacgg gtctcaggat cagactcagg aaatcgctga gtgtttagct
      241 agcaagtatc ctaatatcgt tagagccatc tatcaggaaa ataaatgcca tggcggtgcg
      301 gtcaatcgtg gcttggcaga ggcttctggg cgctatttta aagtagttga cagtgatgac
      361 tgggtggatc ctcgtgccta cttgaaaatt cttgaaacct tgcaggaact tgagagcaag
      421 ggtcaagagg tggatgcctt tgtgaccaat tttgtctatg aaaaggaagg tcagtctcgt
      481 aagaagagta tgagttacga gtcagtcttg cctgttcggc agatttttga ctaggaccag
      541 gtcggaaatt tctccaaagg ccagtatatc atgatacact cgctgattta tcggacagat
      601 ttgttgcgtg ctagccagtt ctaactgcct gaacatactt tttatgtcga tgatctcttt
      661 gtctttacgc cccttcagca ggtcaagacc atgtactatc tgcctgtcga tttctatcgt
      721 tatttgattg ggcgtgagga ccagtctgtc aatgagcaag tgatgattaa gcgcattgac
      781 cagcaactca aggtcaatcg actcttggta gaccagcttg atttgtccca agtgagtcat
      841 cctaaaatgc gagaatatct gctgaatcat attgaaatca cgacggtgat ttccagtacc
      901 ctgctcaacc gatctgaaac agcggagcat ctggcaaaaa aacgccaatt gtggacctat
      961 attcagcaga aaaatccaga agtctttcag gctattcgta agaccatgtt gagccgtttg
     1021 accaaacatt ctgtcttgcc agatcgcaaa ctgtccaatg tcgtctatca aatcaccaaa
     1081 tctgtttatg gatttaatta atataagtgt tttataagag ggatttaaga aaaattttaa
     1141 ctttttctta gtccttttta atttcaggag attatactag agtcatcaaa taaagaaaga
     1201 ctctaaggag aatcctatga aattcaatcc aaatcaaaga tatactcgtt ggtctattcg
     1261 ccgtctcagt gtcggtgttg cctcagttgt tgtggctagt ggcttctttg tcctagttgg
     1321 tcagccaagt tctgtacgtg ccgatgtggt caatccgacc ccaggtcaag tcttacctga
     1381 agagacatcg ggaacgaaag agggtgactt atcagaaaaa ccaggagaca ccgttctcac
     1441 tcaagcgaaa cctgagggcg ttactggaaa tacgaattca cttccgacac ctacagaaag
     1501 aactgaagtg agcgaggaaa caaactcttc tagtctggat acactttttg aaaaagatga
     1561 agaagctcaa aaaaatccag agctaacaga tgtcttaaaa gaaactgtag atacagctga
     1621 tgtggatggg acacaagcaa gtccagcaga aactactcct gaacaagtaa aaggtggagt
     1681 gaaagaaaat acaaaagaca gcatcgatgt tcctgctgct tatcttgaaa aagctgaagg
     1741 gaaaggtcct ttcactgccg gtgtaaacca agtaattcct tatgaactat tcgctggtga
     1801 tggtatgtta actcgtctat tactaaaagc ttcggataat gctccttggt ctgacaatgg
     1861 tactgctaaa aatcctgctt tacctcctct tgaaggatta acaaaaggga aatacttcta
     1921 tgaagtagac ttaaatggca atactgttgg taaacaaggt caagctttaa ttgatcaact
     1981 tcgcgctaat ggtactcaaa cttataaagc tactgttaaa gtttacggaa ataaagacgg
     2041 taaagctgac ttgactaatc tagttgctac taaaaatgta gacatcaaca tcaatggatt
     2101 agttgctaaa gaaactgtcg aaaaagctgt taaagataac gttaaagaca gtat
//
LOCUS       DAVBCN010000131         2142 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVBCN010000131 DAVBCN010000000
VERSION     DAVBCN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.131"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            129..1622
                     /locus_tag="VQ497_002018"
     CDS             129..1622
                     /locus_tag="VQ497_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HET2518283.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            1635..>2142
                     /locus_tag="VQ497_002019"
     CDS             1635..>2142
                     /locus_tag="VQ497_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2518284.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     LQLDELNLL"
BASE COUNT          757 a          322 c          424 g          639 t
ORIGIN      
        1 gtttgaagag attttcgaag agtataaaat attcaggtga cgcatagata tagttaattg
       61 aagctttgtt tgaaatctga taaaataatg atattactaa gttttaaaaa ctaaagaaaa
      121 gggaagatat gattacaggc gaattaaaaa ataaaatcga tcagctgtgg gaaattcttt
      181 ggacagaagg aaacgcaaat cctttaacaa atattgaaca gttgacttat ctcttattta
      241 tgaaagattt ggatagtgtc gagcttggac gtgaaagtga tgctgaattt ctagggattc
      301 cttatgaggg agtttttcca aaagataaac ctgaataccg ttggtcaact tttaaaaata
      361 taggagatgc tcaggaagtt tatcgtttaa tgactcagga gatttttccg tttattaaaa
      421 atctcaaggg ggatacagat gatacagcct tttcacgata tatgcgagaa gctatttttc
      481 aaataaataa acctgctacg cttcaaaagg caatttctat cttagatgtt tttccaacta
      541 ggggattaga tgtagatttt gataatgaca aacaaagtat tactgatatc ggagatatct
      601 atgaatatct gttatcaaaa ttgtcgaccg caggtaaaaa tggacagttc cgtacacctc
      661 gtcacatcat cgatatgatg gttgagttga tgcaaccgac tatcaaagat atcatctcag
      721 atcccgctat gggttctgct ggcttcttag tatctgctag ccgttactta aagcgtaaga
      781 aagatgaatg ggaaaccaat acagataata tcaatcattt tcataatcag atgtttcatg
      841 gaaatgatac ggatacgact atgttgagac ttggggcgat gaacatgatg ctacatggag
      901 tagaaaatcc acaaatcagt taccttgact cgctgtctca agataatgaa gaagccgata
      961 aatatacttt ggttttagca aatcctcctt ttaagggctc acttgactac aattcaacct
     1021 ctaatgacct tcttgcaacc gtaaaaacca aaaaaacaga attactcttt ctttctcttt
     1081 tcttgcgaac tttaaaacca ggtggacgag cagcagttat cgtacctgat ggtgtccttt
     1141 ttggttcgtc taaagctcat aaaggaattc gtcaggaaat tgtagagaat cataagcttg
     1201 atgctgtaat ctcaatgcct agtggtgtgt tcaagcctta tgctggagtt tcaactgcca
     1261 ttctcatctt tacaaaaact ggtaatggtg gtactgacaa agtctggttt tacgatatga
     1321 aagcggatgg tttaagtttg gatgataagc gacaaccgat tagcgacaat gatattccag
     1381 atattatcga acgctttcat catcttgaaa aagaagcaga acgtcagaga acggatcaat
     1441 ctttctttgt tccagttgct gagataaagg aaaatgatta tgatttgtct atcaataaat
     1501 ataaagagat tgagtatgaa aaagttgagt atgaaccaac agaggtcatc ttaaagaaaa
     1561 tcaatgattt agaaaaagaa attcaagctg gcttggctga attagaaaaa ttactcaagt
     1621 agagaggtgg ctgtatgaaa aaagtgaagt tgggggaagt cttatctcta aaaaaaggca
     1681 agaaagccac tgtacttgct gaacaaacaa ctctaagcca acgttatatt caaatagatg
     1741 atttaagaaa taataataat ttaaaattca ctgaaagttt aaatatgact gaagcactcc
     1801 cagatgatat tctgatagca tgggatggag ctaatgcagg aacagttggt tatggattat
     1861 cgggagctgt tggtagtaca attacggtct taaaaaagaa tgagcgatac aaagaaaaaa
     1921 ttatatcaga ttacttggga gtctttttgg aaagtaaatc gcagtattta cgagaacatt
     1981 caacaggtgc aacaattcct catttaaaca agaatatatt acttgattta caattagaat
     2041 tgctaggtat cgaagaacaa gagaacatta tctgtattct taatacgatt aaaaggctta
     2101 ttactaaaag aaaattgcag ttagatgaac taaacttgct cg
//
LOCUS       DAVBCN010000132         2072 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVBCN010000132 DAVBCN010000000
VERSION     DAVBCN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.132"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(71..275)
                     /locus_tag="VQ497_002020"
                     /pseudo
     CDS             complement(71..275)
                     /locus_tag="VQ497_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            632..>2072
                     /gene="pcpA"
                     /locus_tag="VQ497_002021"
     CDS             632..>2072
                     /gene="pcpA"
                     /locus_tag="VQ497_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HET2518285.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDGA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGVVGWVKDKGLWYYLNESGS"
BASE COUNT          749 a          293 c          331 g          699 t
ORIGIN      
        1 ctgtttttaa cacagctaac atctcttcaa aagtggtgcg ctgaacacca acaagacgct
       61 taaatcgtgc atcggttagt tgtttacttg cttcataatt tcgcagggag tctattgact
      121 ctttggtagg tgtcaatgtt tttttcatct atcccgagaa ttattttccc gccatttgta
      181 tttgcaaatg ctgagtaggt ttcccagaaa gactctggaa gattgttttt agcttttttg
      241 tattctaaat caaccccttc aaattttaag tccatatttt tcctttacat ctgttttttg
      301 tggttctggt atttgttcaa gttgagtgat aatatagcga attgaatttc gagagttttt
      361 actcagttaa tttctttttt aacccacttt aatttctttt ttaacccact ttaatttctt
      421 ttttaacacg ggttaaaaaa gaaattaaag tgggttaatt tttcttgact taaaatttaa
      481 aaatgatata ctgtgtttat ggagagatga caatttataa aatgtactac tctattctac
      541 tagataatga ttgaataaaa ctttttataa caacggctag caaagttata attttatatc
      601 tattagaaaa taaatatgta aggaaattta aatgaaaaaa actacaatat tatcattaac
      661 tacagctgcg gttattttag cagcatatgt ccctaatgaa ccaatcctag cagatactcc
      721 tagttcggaa gtaatcaaag agactaaagt tggaagtatt attcaacaaa ataatatcaa
      781 atataaggtt ctaactgtag aaggtaacat aggaactgtt caagtgggta atggagttac
      841 tcctgtagag tttgaagctg gtcaagatgg aaaaccattc acgattccta caaaaatcac
      901 agtaggtgat aaagtattta ccgttactga agtagctagt caagctttta gttattatcc
      961 agatgaaaca ggtagaattg tctactatcc tagctctatt actatcccat caagcataaa
     1021 aaaaatacaa aaaaaaggct tccatggaag taaagctaaa actattattt ttgacaaagg
     1081 cagtcagctg gagaaaattg aagatggagc ttttgatttt tctgaattag aagagattga
     1141 attgcctgca tctctagaat atattggaac aagtgcattt tcttttagtc aaaaattgaa
     1201 aaagctaacc ttttcctcaa gttcaaaatt agaattaata tcacatgagg cttttgctaa
     1261 tttatcaaat ttagagaaac taacattacc aaaatcggtt aaaacattag gaagtaatct
     1321 atttagactc actactagct taaaacatgt tgatgttgaa gaaggaaatg aatcgtttgc
     1381 ctcagttgat ggtgttttgt tttcaaaaga taaaacccaa ttaatttatt atccaagtca
     1441 aaaaaatgac gaaagttata aaacgcctaa ggagacaaaa gaacttgcat catattcgtt
     1501 taataaaaat tcttacttga aaaaactcga attgaatgaa ggtttagaaa aaatcggtac
     1561 ttttgcattt gcagatgcga ttaaacttga agaaattagc ttaccaaata gtttagaaac
     1621 tattgaacgt ttagcctttt acggtaattt agaattaaaa gaacttatat taccagataa
     1681 tgttaaaaat tttggtaaac acgttatgaa cggtttacca aaattaaaaa gtttaacaat
     1741 tggtaataat atcaactcat tgccgtcctt cttcctaagt ggcgtcttag attcattaaa
     1801 ggaaattcat attaagaata aaagtacaga gttttctgtg aaaaaagata catttgcaat
     1861 tcctgaaact gttaagttct atgtaacatc agaacatata aaagatgttc ttaaatcaaa
     1921 tttatctact agtaatgata tcattgttga aaaagtagat aatataaaac aagaaactga
     1981 tgtagctaaa cctaaaaaga attctaatca gggagtagtt ggttgggtta aagacaaagg
     2041 tttatggtat tacttaaatg aatcaggttc aa
//
LOCUS       DAVBCN010000133         1870 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVBCN010000133 DAVBCN010000000
VERSION     DAVBCN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.133"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(408..1604)
                     /gene="tuf"
                     /locus_tag="VQ497_002022"
     CDS             complement(408..1604)
                     /gene="tuf"
                     /locus_tag="VQ497_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET2518286.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
BASE COUNT          533 a          361 c          369 g          607 t
ORIGIN      
        1 gatttcttct ttagttgatt tcatattact attgtataat gctttttgat ttgagtctag
       61 tattatactc ttcgaaaatc tcttcaaacc gcgtcagctt cgccttgtcg tatagatgtt
      121 actgacttcg tcagttctat ctacaaccta aaaacagtgt tttgagcagt ctgcagctaa
      181 ctttctataa caaaatgaaa taagaacaag acaaatcaat caagacaatc aaatcgattt
      241 ctaacaatgt tttagaagta gatgtgtact attctagttt caatatatta tagtttgctc
      301 tttgattttc attgagtatt agttcaatag ctaaaaaaag aaccttgcca agcaagattc
      361 ttttagtgtc tgacttaaat aattgttctt ctgggaacta aatcgaatta agcttcgatt
      421 tctgtaacca tacctgaacc aacagtacgt ccaccctcac ggatagagaa tgtagtacct
      481 tgttctacgg cgattgggtg aatcaactca acatcgattg tcacgttatc accaggcatt
      541 accatttcag tacctgctgg aagttcgatt gaacctgtaa cgtcagtagt acggaagtag
      601 aattgtggac ggtagttgtt gaagaatgga gtgtgacgtc caccttcttc tttagtaagg
      661 atgtagactt cacctttgaa tttagtgtgt gggttgattg aacctggttt agcgataact
      721 tgtccacgtt cgatttcatc acgttgaaca ccacgaagaa ggacacctac gttatctcca
      781 gcaagacctt cgtcaagttg tttacggaac atttcaacac cagtaacaac tgctttttga
      841 gtttcttctt tgataccaac gatttcgatt tcgtcgttga ctttaacgat accacggtcg
      901 atacgtcctg aagcaactgt accacgtcca gtgattgaga atacgtcctc gactggaaga
      961 agcaatggtt tgtcagtgtc acgttctggt tctgggatat actcatcaac tgtgttcatc
     1021 aattccataa cgatgtcttc gtatttagag tcaccttcaa gagctttaag tgctgaacct
     1081 tggataactg gaagatcgtc acctgggaag tcgtattctg acaataggtc acggatttcc
     1141 atttcaacca attcaagcaa ttcttcgtcg tcaaccaagt caactttgtt catgaagacg
     1201 ataaggtgtt taacaccaac ctgacgtgaa agaaggatgt gctcacgagt ttgtggcatt
     1261 ggtccgtcag ttgaagctac tacaaggata gctccgtcca tttgagcagc accagtgatc
     1321 atgtttttaa cgtagtccgc gtgtcctgga gcgtcgatgt gagcgtagtg acgtttttca
     1381 gtttcgtact caacgtgcgc agtgttgata gtgataccgc gttcgcgttc ttctggagca
     1441 gcatcgatag acgcatagtc tttaggttgg ttaactgatg aaggcaagcg acgtgccaaa
     1501 acagttgtga tagctgcagt tagggtagtt ttaccgtggt caacgtgtcc gatagtacca
     1561 atgttaacgt gtggtttact acgatcgtat ttttcttttg ccatttgagt aaaagcctcc
     1621 aataaaatat attttataga tagacagtag gcaatacagt ctaactttcc ttactatttt
     1681 atcaaatttc agctaaattg caagtgtttt acaacgtttt tgtgaattat tcttggtaaa
     1741 aatttaacta attaatagaa agtactaaat agacttatcc ttcaaggagt tttcagcatt
     1801 atccatccac tcacttgctg aaaatgtaaa cagtctctgg tttatatagt aaaatgaaac
     1861 aagaatagag
//
LOCUS       DAVBCN010000134         1860 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVBCN010000134 DAVBCN010000000
VERSION     DAVBCN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.134"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(3..968)
                     /locus_tag="VQ497_002023"
     CDS             complement(3..968)
                     /locus_tag="VQ497_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET2518287.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKVQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            complement(1086..1499)
                     /locus_tag="VQ497_002024"
     CDS             complement(1086..1499)
                     /locus_tag="VQ497_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HET2518288.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKPLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWISEHSVREKVKK"
     gene            complement(1496..1732)
                     /locus_tag="VQ497_002025"
     CDS             complement(1496..1732)
                     /locus_tag="VQ497_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HET2518289.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
BASE COUNT          544 a          362 c          274 g          680 t
ORIGIN      
        1 ttttaagaga tgaatttttg gtgtgaatgc ttgacattat tttgattgac catggcatag
       61 gccagggttg tgtcaatctt gctatgacct agcagttttt ggacttgttc gataggcata
      121 cctttatcaa ttgccttagt cgctaaagtt cttctgaact tatgtgggtg aaccttttgt
      181 atgccgagtt tgtctcctaa ctttcttaag cgaatctcta caccagcaat tccaagcctc
      241 tggacttttc caactagcgt tacaaaaaga gcagggtgac tatcttttct gtcgttaaga
      301 taatttctta aatgaatttt cgtacgagcg tcaaaatata ctggtctctc cttctttcct
      361 ttaccaaaga caacacactc tctgttttca aaatcaatat ctgaacgatt caactgtaca
      421 agctccccta cacgcatacc tgtcgatgct agtaggtcta ttattgccaa atctctcaaa
      481 ttttcacagt tatcacgcat aatttccaaa tgttcatcag tataagtttc cttcacattt
      541 tgctcagtct taattttctt tatccgtcga atgggatttt taatgatata ttcctcttgc
      601 tcaagccaag caaaaaaaga agacaatata cgcctaatat tgtctaaatt tgccttacta
      661 caatcctttt cagactggta atttgctaaa taactcctta aatcatctgt tgtgagttgt
      721 gtcacagact ctttaatagc attaaacaag ttttcaatcg tcctctgata ataacgaatt
      781 gtttttgagc tacagccctc tacctgcttg gcggtaataa atagttgcaa caggttatta
      841 ttcgaatact tctgctgctt ttttccgtct actagatgat gttctaaact ctctctcaac
      901 gctagtaatt gttcattatt taaaagaggt aacatttctt gcatgataat tgtaactttt
      961 gattccataa tttctcactt tcttattcat caataaaaag agacaatatc agtttctgca
     1021 ttacagaatt ctaatattgt ctgttcattt attttcttgt gctcattcaa atccagcata
     1081 ctaggttact ttttgacctt ttctctaaca gaatgttcag aaatccattt ggagtagctt
     1141 gtcatttttt catcagttaa agcttctact gcgatggtta cacacatttt tactgcttct
     1201 tctaccgtta cagaaaaacg atagccgttt aattgtaaaa atttgaccaa aacgaagaaa
     1261 gcagttcttt tattagcatt agcaaaaaca tgcttcttta tcaattggac aaatagtatc
     1321 gttgctttat caaaaattgt tggataaaga ggcttcccaa agacaaattg ttctggtaag
     1381 ttcacaatca tatttaaggc agaagaacta actgtttgaa ttttctcatt tggagaatac
     1441 cgttgaattg ctaaagcatt tattttttca atttgctttt ctgtcaaata gattgtcatt
     1501 tttccaccaa agctttgaaa acatcgtcgt attcgtcaaa tattttatct aaatttaagt
     1561 caaaggcatc atcagataca taagaaacct gctgccccaa ttcttcagga gtaaagataa
     1621 tctcaccact tggtaacaat ttcgcttcgt atttaacacc acttggaata ttaaattcac
     1681 ttggaatcgt aatggtgatt gaatttcctt gttttcttgt ttttactacc atctgatttc
     1741 ctcctttgaa gtattataac aaatcattac cgtaattaca atggcagaat atcagccaaa
     1801 atactcctgc atcagagatt tcttcaaagt ttcaagttct tccagagatt tttggattgc
//
LOCUS       DAVBCN010000135         1790 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVBCN010000135 DAVBCN010000000
VERSION     DAVBCN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.135"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            84..491
                     /locus_tag="VQ497_002026"
     CDS             84..491
                     /locus_tag="VQ497_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2518290.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATSFKEVQKPLENFN
                     HIAWFPIDEAIQNLKRGSHKWAIESWKKQHKIG"
     gene            656..955
                     /locus_tag="VQ497_002027"
     CDS             656..955
                     /locus_tag="VQ497_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HET2518291.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVNEANIKTLYSEKLIGISIPIK"
     gene            1216..1539
                     /gene="tnpB"
                     /locus_tag="VQ497_002028"
     CDS             1216..1539
                     /gene="tnpB"
                     /locus_tag="VQ497_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET2518292.1"
                     /translation="MMIVLSDLGQVHLVCGKTDMRQGIDSLAYVVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENGRLTWPSTEKDVKALTSEQVDWLMKGFS
                     ITPQI"
BASE COUNT          600 a          338 c          313 g          539 t
ORIGIN      
        1 actataataa taacgatact agataatgag gaaaatgcaa tggcaaaccc aactttcgga
       61 gaaaaaaagc gaatacagac tatattgctc gctatggcgt atatgcagtt atccctaatc
      121 ctgaacaaaa acaaattgtt cttgttcaag aaccaaatgg tgcttggttc ctaccatgtg
      181 gagaaattga agcaggtgaa aatcatcagg aagccctaaa gcgtgagttg attgaagagc
      241 ttggtttcac agcagaaatt ggtacctatt acggacaagc tgacgaatat ttctattctc
      301 gtcatcgtga tacctactac tacaatcctg cctacctcta tgaagcaact tctttcaaag
      361 aagtacaaaa gccactagaa aactttaatc atattgcctg gttccctatt gacgaggcta
      421 tccaaaacct taaacgtggt agccataaat gggccattga atcttggaaa aaacagcata
      481 agattggcta atactcttcg aaaataaaat tcaaaccacg tcagcgtcgc cttaccgtac
      541 tcaagtatag cctgcggcca gcttcctagt ttgctctttg attttcattg agtataaatc
      601 aagcgacttt attcaaaaag tgctataata gaattagaaa atcggaggaa atattatgaa
      661 gcttatcaat acgacaaact cacactcgca acttgtaaaa agtcaactag aaagtaccga
      721 tgcaaccctt gttgaggttt attctgcagg gaatacagat gttattttta cccaagctcc
      781 gcttcactat gaaatcctca tctcaaacaa acaccgtgct attcgtgagc ctgaaatcga
      841 aaccattcaa gaatttttct tgaaacgtaa aattgataag acctctgtta atgaagccaa
      901 tatcaagaca ctctactcag agaaattaat tggaatttca atcccaatca agtaagaaaa
      961 ttgagtaaaa actcgatttg agagaaagtc caataagtct ttccataaca aaacgcatag
     1021 taacaagagt ttctacaccc actattatac gtttttatca ttttgaaatc tgctgctaat
     1081 caatgaagaa gcaaagatca aactaggcag ctagttacaa gttacgtaag cgcgtcataa
     1141 caaggtatct atcctaattc cccaacttcc agtatactag ataaaaaata attatcagag
     1201 gaaaggtatt gtactatgat gattgtacta agtgatttag gtcaagttca ccttgtttgt
     1261 ggcaagacag atatgaggca gggaatagac tcattagcct atgtagttaa aacccacttt
     1321 gaattggatc ctttctccgg tcaagtcttt ctcttttgtg gtggacgtaa agaccgcttt
     1381 aaagcccttt actgggatgg tcaaggattt tggctactat ataaacgctt tgagaacggc
     1441 agactgactt ggcccagtac agaaaaggat gtcaaagctc tcacatctga acaagtagac
     1501 tggcttatga agggcttttc tatcactccc caaatatagt ggatttaaac tagaatagta
     1561 cacctctact tctaaaacat tgttagaaat cgatttgtcc tgttcttgtt tcattttact
     1621 atagaacgat ttgaaggcgt ttatgatatt tagctgtacg agagtctttt aaaagtgttt
     1681 tgatggtttg gatttcttct ttaattgatt tcatattact attatataat acttttttat
     1741 tttagtctag tataaatagc gagaaatatc tatcctatct tctagaatgt
//
LOCUS       DAVBCN010000136         1668 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVBCN010000136 DAVBCN010000000
VERSION     DAVBCN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.136"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            100..1575
                     /locus_tag="VQ497_002029"
     CDS             100..1575
                     /locus_tag="VQ497_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HET2518293.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNIVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
BASE COUNT          472 a          335 c          334 g          527 t
ORIGIN      
        1 tccagtattt gacaatattg aagcagctat taaagaacac ggattaagct aattctaatt
       61 tgttaacatt catataacat aaaaaaggga ggaactgtta tggatgcaat ctttgaccta
      121 atcggaaagg ttttcaatcc cattttagaa atgggtggac ctgtcatcat gttaatcatt
      181 ttgacagtat tggctttact ttttggagtg aaattctcca aagcgcttga aggtggtatc
      241 aaacttgcca tcgctcttac aggtatcggt gctatcatcg gtatgctaaa tggtgctttc
      301 tcagcatcac ttgcaaaatt cgttgaaaac actggtatcc aattgagtat taccgacgtt
      361 ggttgggcac cactcgcaac aatcacttgg ggttctgctt ggacactata cttcttgctc
      421 atcatgttga ttgtcaacat agtgatgcta gctatgaaga aaacagatac acttgacgtt
      481 gatatctttg atatctggca cttgtctatc acaggtctct tgattaaatg gtatgctgat
      541 aacaatggtg tgagtcaagg ggtttcactc tttattgcta cagcagctat cgtccttgtc
      601 ggtgtgttga aaattatcaa ctctgacttg atgaaaccta catttgatga ccttcttaac
      661 gccccaagtt catcaccaat gacatcaact cacatgaact acatgatgaa cccagttatc
      721 atggttttgg ataagatttt tgaaaaattc ttcccaggcc ttgataaata tgactttgat
      781 gctgctaaat tgaacaagaa aatcggtttc tggggatcta aattcttcat cggtttcatc
      841 cttggtatcg ttatcggtat tatgggaact ccacatccaa ttgcaggtgt tgcagatgca
      901 gataaatggc gtcttgttat caaaggatgg ttgtctcttg gtttgactgc cggtgtatct
      961 ttggaactct tctcacttat cggttcatgg ttcatcgcag ccgtagaacc actatcacaa
     1021 ggtattacaa acgttgctac taaacgtctt caaggacgta aattcaatat cggtcttgac
     1081 tggccattca tcgctggtcg tgctgaaatc tgggcttgtg ccaacgtact tgcaccaatc
     1141 atgttgattg aagcagtgct tctttcaaaa gttggaaatg gtatcttgcc acttgcaggt
     1201 atcatcgcta tgggtgttac tccagctctc ttggttgtaa ctcgtggtaa attgctccgt
     1261 atgattatct tcggaacact cttgttgcca ctcttccttc tttcaggtac acttattgca
     1321 ccatttgcaa cagaacttgc taaaggtgta ggtgccttcc cagaaggtgt gagccaaact
     1381 caattgatta ctcactctac tcttgaagga ccaatcgaaa aacttcttgg ttggacaatt
     1441 ggtaacacta caactggtga tatcaaagca atccttggtg cagtagtctt ccttgtattc
     1501 tatatcggta tctttgcttg gtacagaaaa caaatgatca aacgtaacga agagtacgca
     1561 gcaaaagcaa aataatgcgc tcctataaaa tataaactca atgaaaatca aagagcaaac
     1621 taggaaacta gccacaggtt gctcaaaaca cagttttgag gttgtaga
//
LOCUS       DAVBCN010000137         1640 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVBCN010000137 DAVBCN010000000
VERSION     DAVBCN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.137"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            110..409
                     /locus_tag="VQ497_002030"
     CDS             110..409
                     /locus_tag="VQ497_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic biosynthesis protein"
                     /protein_id="HET2518294.1"
                     /translation="MDSYAINFWESKGVGIDKKILEFIQKNITVIKNKNFLNKDFDIY
                     VKKIHNLFFVISTLVSKEQYMGIVSSQIHMFANRMGISPTIEVTMLDFFLREDSD"
     gene            411..1181
                     /locus_tag="VQ497_002031"
     CDS             411..1181
                     /locus_tag="VQ497_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HET2518295.1"
                     /translation="MEKYFFDISIFEKIKKVLSYQSKYWEISKPRDKYSFKSQNRISG
                     SNSIKFKDFELDYELESLGKILLKRRSFLLNEFDKNASFDKLIYLLKTVFSPTNCKFV
                     FGEEVPLFSYPTSGALNSITPFVYIDNMDEVKNGIYEVKYDGLEFKKSMNIIDLETFT
                     PITSKNSDVQNNSFKKVNMIIMLCSNYENIFNKYGLLAYRLLHLEAGHIAQNIQLVST
                     ALGIKSLPMGGWFDDKIETFINEISDKKNIYLYAIAVG"
     gene            1201..1365
                     /locus_tag="VQ497_002032"
     CDS             1201..1365
                     /locus_tag="VQ497_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518296.1"
                     /translation="MNAEDKFRELENENKLLKLHNKELENEIQNGWKFWIWVTVPILG
                     LIYAIIQKIF"
BASE COUNT          602 a          180 c          268 g          590 t
ORIGIN      
        1 agataactat tattacgaaa aaaaattctt gtgtaaaatt ctattgactt tctatattat
       61 ttttgtattt cataacgaat gtaatttaac aagtgaagaa attttaaaga tggattccta
      121 tgctattaat ttctgggaga gtaaaggggt cggtattgac aaaaaaatct tggaatttat
      181 tcaaaaaaat attacagtta ttaaaaataa aaattttttg aataaagatt ttgatattta
      241 tgttaaaaag attcataatt tattctttgt catttctact ttagtttcaa aagagcaata
      301 catggggata gtttcttccc aaatacatat gttcgcaaat aggatgggaa tttctcccac
      361 cattgaagtt accatgctag atttttttct gagggaggat tctgattaat atggaaaaat
      421 atttttttga tatttctatt tttgaaaaaa taaaaaaagt attatcatac caatctaaat
      481 attgggaaat atcgaagcct agggataagt acagtttcaa aagtcaaaat agaatatctg
      541 gctctaatag cattaaattt aaagattttg agttagatta tgaactggag tcacttggta
      601 aaatactttt aaaaaggaga tcgtttttac taaatgaatt tgataagaat gcgagttttg
      661 acaaactgat ttacttgttg aaaactgtat tctctcctac aaattgcaag tttgtatttg
      721 gagaagaggt tcctttattt agctatccta catcaggtgc gttaaattca attactcctt
      781 ttgtttatat tgataatatg gatgaagtaa agaatggtat atatgaagta aagtatgacg
      841 gacttgaatt taaaaaaagt atgaatatta tagacttaga aacatttacc ccaataacaa
      901 gtaaaaacag tgacgtacaa aataattcat ttaaaaaggt aaatatgata attatgttat
      961 gttcgaatta tgaaaatatt ttcaataaat atggattatt agcttatcga cttttgcatt
     1021 tagaggctgg tcatattgca caaaatatcc agcttgtttc tactgcgcta ggaataaaaa
     1081 gtttaccgat gggaggttgg tttgatgata aaatagaaac ttttattaat gaaattagtg
     1141 ataaaaaaaa tatttatctc tatgcaattg cagttggata aatttaagga ggaagtgtag
     1201 atgaacgcag aggataaatt tcgcgaacta gaaaatgaaa ataaacttct aaaacttcat
     1261 aacaaggagc ttgagaatga aattcaaaat ggatggaaat tttggatatg ggtaactgtc
     1321 cctattttag gtttgatata tgctattatt caaaaaattt tttaattatg ctagtttgtc
     1381 aggttgagta gtttttgttc aaaatttaat aaactttttt caatgaagat tatctaaaga
     1441 ttgcttctca gatgagaatg gcttctctat cttatataaa ttatcttcat agagtgtcct
     1501 gttattcatg ttatcacaga atagatagtt tttttggctc tataatattt gtagtgggta
     1561 aatcccctat agatattatg gagcctattt ttgtgtagaa aaaaagtccc atatcatcta
     1621 taatgaaaag caaccaaact
//
LOCUS       DAVBCN010000138         1625 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVBCN010000138 DAVBCN010000000
VERSION     DAVBCN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.138"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(143..283)
                     /locus_tag="VQ497_002033"
     CDS             complement(143..283)
                     /locus_tag="VQ497_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518297.1"
                     /translation="MKFFWTILAILFIKPIIGIVKFFWMIISFAVQLLFYKILDWFFK
                     LI"
     gene            complement(280..462)
                     /locus_tag="VQ497_002034"
     CDS             complement(280..462)
                     /locus_tag="VQ497_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518298.1"
                     /translation="MNPIKAFAKIYGNYFLTVQGVKVMKTIKKDDHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(464..1247)
                     /locus_tag="VQ497_002035"
                     /pseudo
     CDS             complement(464..1247)
                     /locus_tag="VQ497_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          547 a          331 c          245 g          502 t
ORIGIN      
        1 tacagcaagg tgaagctgac gtggtttgaa gagattttcg aagagtataa aatgatttta
       61 ctgcttatat tataccatat ggagagagat taaaaaacta gcagtggagt tcctgctagt
      121 tttctgcgac ttggattacc atctagataa gtttaaagaa ccaatccaat atcttgtaaa
      181 acagcaattg aactgcaaaa gaaatgatca tccaaaagaa tttcacaatc ccaataatcg
      241 gtttgataaa aagaatagca agaattgtcc agaaaaattt cattctctct cctctggctt
      301 aatgagccac cgagccgtat ccattagctt atctgcaata aagagttttc ccagaccaac
      361 aacgacatgg tcatctttct ttatcgtttt catcactttt acaccttgca cggtcaaaaa
      421 gtaattacca taaattttag caaaagcttt gataggattc atattattct cctttgatga
      481 tttcagcctc ttttcggatt gtttcaacat cttcttgata ttgaacttca ctatagttga
      541 ctagcttgga taattctttt tgcaagcttt cccccattgg tttcatagtt gcaaaggtta
      601 aaccacctga aatgatacca cccaagatag gaataaattt ccccattcct ttggccaacc
      661 ctcccttggt aagattcaca ccaaatattt ttaagacttt tttcaaaata gggtaccaaa
      721 gcgtctttgt taaagcttta ttaggcactg ttttcattac ctgtttggca attgttatac
      781 caccagcacg tagcaaacaa agcagcggtt ccattcaccc ctaacattac gcctagataa
      841 agcaagaggg tattttgagc atcttcactc aactcctcac gtgaagccca aagatcctca
      901 taaccataaa tataacctaa ttcttgggcc aatttcagag agaaaccata aaattgagcc
      961 acataagctg gaatggtaat tgccatagca agccctccag gtaatcctgc caaaacagaa
     1021 gtcccactcg ctaataaaac attatcccta atacaggcat tagccactcg atctaagatt
     1081 tcttgagata agagagacgt tggaccttgt tccaataagg taggaatgtc ctgtggaccc
     1141 aattctttgg aaaacttatc cactaaaaat ttcgaacgat caaccttaac aactggtagt
     1201 tttaccactt gttgtaatac ttgcatagct aagtcttgtt cagccatata caaactcctt
     1261 atttcttttt tgcaccttat catattatat ttcctacaaa aaaagcattt tctaattaga
     1321 taacattaaa aaatacagaa cccctttttt atagtaaaat aaaacaagaa caggacaaat
     1381 cgatcaggac agtcaaatcg atttctaaca atgttttaga agtagaggcg tactattcta
     1441 gtttcaatat attttaaaag aatatttttc catttcaaat tcaacctatt ttcctccacc
     1501 aaaaaagggc tctataatat ttgtagtggg taaattccct atggatatta tggagcctat
     1561 ttttgtgtag aaaaaaagtc ccatatgacc tataatgaaa agcgacaaaa caactcatta
     1621 gaaag
//
LOCUS       DAVBCN010000139         1538 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVBCN010000139 DAVBCN010000000
VERSION     DAVBCN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.139"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(6..605)
                     /locus_tag="VQ497_002036"
     CDS             complement(6..605)
                     /locus_tag="VQ497_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HET2518299.1"
                     /translation="MTFCAGYVDAYTFIVRGNTLVAGQAGNVVFLSVELIQNNVSDVR
                     DKLLTLITFIMGVFLLTIYKEKLRIVKKPILSLIPLAILSIIIGFVPQTVDNIYLVQP
                     LAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLAFGDYFRTKHTPFLREGFIFVSLL
                     SSFVLGDVFSAYLTIFYHEKTILGVPIMMSIFYLSMLFA"
     gene            1075..1374
                     /locus_tag="VQ497_002037"
     CDS             1075..1374
                     /locus_tag="VQ497_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET2518300.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT          577 a          293 c          243 g          425 t
ORIGIN      
        1 tgccactagg caaaaagcat actgagataa aaaatgctca tcataatagg aacaccaaga
       61 atggtctttt catgatagaa aatcgtcaaa taggctgaaa agacatcgcc aaggacaaaa
      121 ctactaagca ggctaacaaa tatgaaccct tcacgcaaaa aaggagtgtg cttagttcgg
      181 aaataatctc caaaagctag cattgttcgt ttgatattcc ctgtcataaa ggcattattg
      241 taggcaatac ccgaaacctc accaaaagca gttgtcacca gtcccataca gaaggccaag
      301 ggctgcacta gatagatatt atctacagtt tgtggcacaa aaccaataat tattgatagg
      361 attgctaaag gaatcaagga cagaataggt tttttcacaa ttctcaattt ttccttataa
      421 atcgttaata aaaagactcc cattataaac gttattaagg ttaaaagctt atccctaaca
      481 tccgaaacat tattttgtat tagctctacc gaaagaaaga caacatttcc agcttgtcca
      541 gctacaaggg tattccctcg aacaataaaa gtgtaagcat ccacatatcc agcacaaaat
      601 gtcaaaaaag tgctaacctt ttagactgac gtgatatttt tcttataggt aataacctca
      661 ttttccctcc cattgtattt tctcttagaa atattgtacc atttctttct aaaaaatcgt
      721 agactaccat ttagatttta ctattagcat aaaaataata atagacaact atttatccaa
      781 aaatagatag aggtaacatg tttgcaaaca aagcatacga acctttagta aaatcatttc
      841 catgaaacta gaatagagcc cctcttagca aaaatcatta ttttaattta tttctaatca
      901 ctccttgaca taaataactc tcaccaataa aagactatgt cttaaaaaaa tggtataata
      961 aaatcaatac ctgggcttga tggctatgct actaataaca attaggagag aaaatcaggc
     1021 acttgttaac aacaaggatt atccccttga gatgaaagga actttagaaa tcttatgatg
     1081 aacatgcaaa acatgatgcg tcaagcacaa aaacttcaaa aacaaatgga acaaagccaa
     1141 gctgaacttg ctgctatgca atttgttggc aaatctgctc aagatcttgt ccaagcgacc
     1201 ttaactggcg ataagaaagt tgtcagcatt gatttcaatc cagctgtcgt tgacccagag
     1261 gaccttgaga ctctttctga tatgaccgtt caagccatca actctgctct tgaacaaatc
     1321 gatgaaacta ctaagaaaaa actgggtgct ttcgctggga aattgccatt ctaaaaacaa
     1381 ggagctagaa caatacttgt cgataacaaa ggctaagaaa ggtgcaaaaa ttataaaaaa
     1441 cgcatagtat caagcaacct aaaactttgg gctctataat atttgtagtg ggtaaatcca
     1501 ctatagatat tatggagcct attttattgt agaaaaaa
//
LOCUS       DAVBCN010000140         1450 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVBCN010000140 DAVBCN010000000
VERSION     DAVBCN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.140"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            1..747
                     /locus_tag="VQ497_002038"
                     /pseudo
     CDS             1..747
                     /locus_tag="VQ497_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878975.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chordopoxvirus G3 family protein"
BASE COUNT          286 a          325 c          472 g          367 t
ORIGIN      
        1 ctggtacttg ctgaagccga agcactggta cttgctgaag ccgaggcgct tgtcaatgct
       61 gaggcagacg ttgatgcgga ttcagacgca ctcgttgatg ctgaggctga agcacttgtt
      121 gaagctgatg tacttgctga tgtcgacgcg cttgttgatg cagaagctga gatacttgct
      181 gaagctgagg cactggttga tgctgaagct gaggcgctgg tgcttgctga agccgaagca
      241 cttgttgagg ccgattcaga cgctgaggta cttgctacag atgacttgga aacttctgta
      301 cctgtatttg gcaattgctg tcttgactta cctgctgaag ccgaggcgct ggtgcttgct
      361 gaggctgagg cgctggtact tgctgaagcc gaggcgcttg ttgatgctga agccgacgca
      421 ctcgttgatg cggaagctga tgcactggtt gacgctgaag ccgacgcact cgttgatgca
      481 gattcagacg cacttgcgct tgttgaagcc gatgtactta ctgaggtcga cgcgcttgtt
      541 gatgccgacg tgcttgctga ggtcgacgcg cttgttgatg ctgaagctga cgcactggtg
      601 cttgctgaag ccgaggcgct cgttgatgcc gattcagacg ctgagatact tgctgaggct
      661 gatgcactcg ttgatgctga agctgaagca ctggtacttg ctgaggctga tgcactggtt
      721 gatgctgagg ctgacgcact tgtacttgct gaggccgagg cactcgttga tgctgaagct
      781 gacgcactgg tgcttgctga agccgaggcg ctcgttgatg ctgaagctga cgcacttgtg
      841 cttgctgaag ctgacgcact tgtacttgct gaagccgaag cactcgttga tgctgaggct
      901 gaagcactgg ttgatgccga ttcagaggca ctggttgacg ctgaagccga cgcacttatt
      961 gatgcagatt cagaagctga tgtacttgct gaagccgaag cgcttgtcga tgctgaggct
     1021 gaagcactgg ttgatgctga ggctgaggcg ctggttgacg ctgaagctga ggcagacgtt
     1081 gatgctgaag ccgatgcact cgttgatgct gaagccgacg cactcgttga tgctgaagct
     1141 gaggcgctgg ttgacgctga agctgacgca cttgtgcttg ctgaagctga cgcactggta
     1201 cttgctgaag ccgaggcact tgttgatgcg gaagctgagg cgcttgtgct tgctgaagcc
     1261 gaagcactcg ttgacgctga ggctgacgca ctcgttgagg ccgattcaga agctgaggtg
     1321 cttgctgaag ctgaagcact cgttgatgct gaagctgatg cactagtact tgctgaggct
     1381 gaggcactcg ttgatgctga ttcagaagct gatgtgcttg ctgaggctga agcactggta
     1441 cttgctgagg
//
LOCUS       DAVBCN010000141         1320 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVBCN010000141 DAVBCN010000000
VERSION     DAVBCN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.141"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            236..676
                     /locus_tag="VQ497_002039"
     CDS             236..676
                     /locus_tag="VQ497_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2518301.1"
                     /translation="MKVELQISETYEEEKLIVQAPQPTDKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            673..1131
                     /locus_tag="VQ497_002040"
     CDS             673..1131
                     /locus_tag="VQ497_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HET2518302.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
BASE COUNT          419 a          287 c          248 g          366 t
ORIGIN      
        1 ccttgccaga gggagtaaaa tagtggtata ctcaataaaa atcaaagagc aaactagaaa
       61 gctaggcgca gacagctcaa aacactgttt tgaggttgta gataaaactg acaaagtcaa
      121 caactatcct acggcaagac gacgctgacg tggtttgaag agatttttga agagtattaa
      181 taaaggtgta caagaagtga gattaatttt gtatgaaaaa caaggagaga aatttatgaa
      241 agtagaatta cagattagtg agacttacga ggaggaaaag ctgattgtcc aagcacctca
      301 gccgacagat aaagttcaga aagtcatcga gttcgcagaa aatctggacc aaagagaaaa
      361 aatcaaaggg aaaatcgatg atcaggtcta tctagttgag attggcaaga ttcagcgctt
      421 ctatatcgag aatcggaagg ttctagcaga aaccgcgagt cagacctaca gcattgattt
      481 gcgactctat caggttctta aactcttgcc aaccaatttt atccaaattt cccaatcaga
      541 aatcatcaat atcgattcca tctctcacct caagctcacg ccaaacggtc tggtagaaat
      601 tttcttgaaa aacgaaagct tcacctactc ttcacgccgt tatctaaaaa ccatcaagga
      661 gaaattagaa ctatgaaaaa acaagtattt cacgatgcag ctgccggtgt tcttatcggc
      721 ctcatcctct ctatcctctt ttcactcatt tatgcaccaa atacctacgc accactaaat
      781 ccctactctc tcatcggcca agtgatggat cagcatcagg ttcacggtgc cttggtcttg
      841 ctctactgca cacttatctg ggcagccatt ggtatgctct tcaactttgg caatcgctta
      901 ttcagccgtg actggagcat gcttcgtgcc actctgactc atttcttcct tatgctggct
      961 ggctttgtcc cactagcaac tcttgctggt tggttccctt tccactggat tttctacctc
     1021 cagctcatta tcgagtttgc gattgtctat ctcatcatct gggctattct ctataaaaga
     1081 gaggctaaaa aagtagatca catcaatcaa ctcttggagc atagaaaata ggaacctcat
     1141 aataagcaaa tccgagaaag aaacttctcg gtttttcttt atcctgctgc accttactat
     1201 agtgaaatga aataagaaca ggataaatcg atcaggacag tcaaatcgat ttctaacaat
     1261 gttttagaag cagaggtgta ctattctagt ttcaatcctc tatatcataa cagttatttc
//
LOCUS       DAVBCN010000142         1183 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVBCN010000142 DAVBCN010000000
VERSION     DAVBCN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.142"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(624..854)
                     /gene="blpU"
                     /locus_tag="VQ497_002041"
     CDS             complement(624..854)
                     /gene="blpU"
                     /locus_tag="VQ497_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HET2518303.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAATCWW"
     gene            complement(1057..>1183)
                     /locus_tag="VQ497_002042"
     CDS             complement(1057..>1183)
                     /locus_tag="VQ497_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET2518304.1"
                     /translation="ENIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
BASE COUNT          369 a          255 c          147 g          412 t
ORIGIN      
        1 agataggact gacgaagtca gtcacatata tacggcaagg cgacgttgac gtggtttgaa
       61 tttgattttc gaagagtata aactcatctg ataacaacta tcttacccca atcactaaac
      121 aaaaactgga cttccttccc aaatgagcct atctccctcc caactgcact ttttggataa
      181 aaaaataggc agaacaagag tcctgcccac taaaaatatg ctattgaaag gaaattgatt
      241 ttttactgtc accctaggca agcttgaaac aaataaatca ttgatagtaa cacttacgac
      301 atcctattta ttacttcaat ttctaatgtc cctgctattt ctaagctacc tctggtatga
      361 tctattccta gtcagaatag ataacaccgt tctaaattac gattcatagt tactcctttt
      421 ttgactcgtt actataagac tcctctttac atgcgcaaaa taattttgat tcagattcat
      481 catttatagt tttgattatt ttttaacaga tttttctgtt ttcttctccg aagactttaa
      541 aaattttttt ctaattaaat catccatata aggaccaaat ataccaacta ctaaaccaat
      601 aataaaactt ttaaaatcca taattaccac caacatgtcg ctgcataggc cacacctcca
      661 agtatagctc ctcccgcagc accagttgct gcaccttgcc atgttcttgt tttaattcct
      721 agctgaagac ctcgtactgc tcctcctcca acacctgctt tggcaaaatc tccccaattg
      781 catccgccac cttcaacgca agcaagcatc tcagtatcca taatttcaaa ttgtgacatc
      841 ttttttgtat tcataacgaa tacctctttt ttatttttaa tatttgtctt gttacaaact
      901 tgacaagttt agtataacag tatctattaa tttttttcat ccaaatcttg aattggcatc
      961 gaaacgtctt gaattagctt ttttgtttca aaatcatctc tatttttaaa aaagatgttt
     1021 tctaatcact tttttactat tagacttcct gcaaaactag aatcctagtt catgattgat
     1081 aataccagca atcaaattca ttcgtaatcc gaagcgttta cgatgatttc gataggttgt
     1141 tgaaaacatt ttaaacgttt ttactttggc aaagatattc tca
//
LOCUS       DAVBCN010000143         1160 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVBCN010000143 DAVBCN010000000
VERSION     DAVBCN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.143"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(<1..107)
                     /locus_tag="VQ497_002043"
     CDS             complement(<1..107)
                     /locus_tag="VQ497_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2518305.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASL"
     gene            complement(565..963)
                     /locus_tag="VQ497_002044"
     CDS             complement(565..963)
                     /locus_tag="VQ497_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2518306.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLQIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
BASE COUNT          351 a          222 c          174 g          413 t
ORIGIN      
        1 agagaggcaa tttctctatt tgattttcct tcttttttcc atcgttcgat taagcgacgg
       61 ctatcgattg tcaaatgttt ggcttttgta gtataattgt cttgcatctc tgtgcctttc
      121 ttgtgtttgt ggttgaacaa caagtataac acagaggtgt tttcttatgc ctacaagagt
      181 tttcattatt tccattttct ttcggatttc actctattct gaaaaacttg tgtatattta
      241 ctgaagctag caagtcttac ctgtaaattt aatgaaagca acacaaaatc ctagagggga
      301 atctcggatt aatgtcaatt taaaaagata gagagttttt agtttaaata aattgtttta
      361 aaatatcaac aacatcactt cttttcttaa cctgataagc cttgattcct aattttggag
      421 ctacgattat attgtcctca atatcgtcta gaaagacaca atttctaggt tataactggt
      481 atttatcgat agttactcat atacatcagt ccacctccat acttatgtgc gagcctctct
      541 ttgtattata cctccatact caccttacag attcttttgg taataatatc tttgcctaat
      601 gtagagatag tcttgcaaag aaaaaacttc cttgtagcca tgtttctgat aaaagtccgg
      661 tgcctggaac tgataagtat tgacaaaggc aaaacaacaa tttcgattct tagcttcact
      721 ttctgcctgt tgcaatagtt ttgaaccgat tccttgccct cgcagttcct cttttacaaa
      781 caaatactcg atttgtagcc aatttccaaa agtctctgct atcaaacccg caaggagatt
      841 gcccttttca tcttcgacat aaagattaag tggctcactt tcagcctctt ccctttttga
      901 acggttataa acacgaatca gattccctat ttcttgcgat ttatgtgatt ccttattttc
      961 caatctaaag tataatgaaa tgaaataaaa catgcgcaaa tcgattaagg aatttaatct
     1021 aatttctaat aatgtcttag aaatcaaagt ttactatttt aacttcaatg cactatacat
     1081 ctaatactca atgaaaatca aagagcaaac taggaaacta gccgcaggtt gctcaaaaca
     1141 ctgttttgag gttgtagata
//
LOCUS       DAVBCN010000144         1081 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVBCN010000144 DAVBCN010000000
VERSION     DAVBCN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.144"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..>1081
                     /locus_tag="VQ497_002045"
     rRNA            <1..>1081
                     /locus_tag="VQ497_002045"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          315 a          213 c          325 g          228 t
ORIGIN      
        1 gggacacgtg aaatcccgtc ggaatctggg aggaccatct cccaacccta aatactccct
       61 agtgaccgat agtgaaccag taccgtgagg gaaaggtgaa aagcaccccg ggaggggagt
      121 gaaatagaac ctgaaaccgt gtgcctacaa caagttcgag cccgttaatg ggtgagagcg
      181 tgccttttgt agaatgaacc ggcgagttac gttatgatgc gaggttaagt tgaagagacg
      241 gagccgtagg gaaaccgagt ctgaataggg cgccttagta tcatgacgta gacccgaaac
      301 catgtgacct acccatgagc aggttgaagg tgcggtaaga cgcactggag gaccgaacca
      361 gggcacgttg aaaagtgctt ggatgacttg tgggtagcgg agaaattcca aacgaacttg
      421 gagatagctg gttctctccg aaatagcttt agggctagcg tcgacattag agattcttgg
      481 aggtagagca ctgtttgggt gaggggtcca tcccggatta ccaatctcag ataaactccg
      541 aatgccaatg aattatggtc ggcagtcaga ctgcgagtgc taagatccgt agtcgaaagg
      601 gaaacagccc agaccaccag ctaaggtccc aaaataattg ttaagtggaa aaggatgtgg
      661 ggttgcacag acaactagga tgttagctta gaagcagcta ttcattcaaa gagtgcgtaa
      721 tagctcacta gtcgagtgac cctgcgccga aaatgtaccg gggctaaaac aatttaccga
      781 agctgtggat acctttatag gtatggtagg agagcgttct atgtgtgatg aaggtatacc
      841 gtgaggagtg ctggaacgca tagaagtgag aatgccggta tgagtagcga aagacaggtg
      901 agaatcctgt ccaccgtaag actaaggttt ccaggggaag gctcgtccgc cctgggttag
      961 tcgggaccta aggagagacc gaaaggtgta tccgatggac aacaggttga tattcctgta
     1021 ctagagtatg tagtgatgga gggacgcagt aggctaacta aagcagacga ttggaagagt
     1081 c
//
LOCUS       DAVBCN010000145         1054 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVBCN010000145 DAVBCN010000000
VERSION     DAVBCN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.145"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..555
                     /locus_tag="VQ497_002046"
     CDS             <1..555
                     /locus_tag="VQ497_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2518307.1"
                     /translation="KDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGTE
                     IPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIPA
                     THNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPIS
                     NHEEMKRIISKVDLLFQKVSQLFE"
     gene            complement(503..1048)
                     /locus_tag="VQ497_002047"
     CDS             complement(503..1048)
                     /locus_tag="VQ497_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2518308.1"
                     /translation="MNWVVIKIKDIFSINTGLSYKKGDLSINNKGVRIIRGGNIKPLE
                     FSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIF
                     QLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFE
                     EQELITQKVEKLFEKVNQLWK"
BASE COUNT          380 a          165 c          153 g          356 t
ORIGIN      
        1 aaagacgaaa caacctctta tcctatatat gaaatcccag aagcatggag atatattaaa
       61 tttgctagcc tagttaactt cagaatagga aaaactcctc cacgcagtga agctactttt
      121 tggggaactg aaataccttg ggtatctata tcagatatgc ctatatctgg ttatgtaact
      181 aatacgagag aatctatttc taaattagca ttaaaatcaa agaaaataga catttcacct
      241 aaaggaactt tattaatgag ttttaaatta tctattggaa aagttgccat attggatatt
      301 cctgccactc ataatgaagc cattatatcg atattcccct atgctaataa agaaaatatt
      361 atcagagatt acttaatgat atttttgcca cttatctcta ctttaggtga ttcaaaagat
      421 gctataaaag ggaaaacatt aaatagtacc agtatctccg aattattaat ccctatttct
      481 aatcatgaag agatgaaaag aatcatttcc aaagttgatt tactttttca aaaagtttct
      541 caactttttg agtaataagt tcctgttcct caaaaggagc taacggaatt aatagctcgc
      601 ttagtgtagt tttaggaata ttatataaag cttgacctga tagtttagtt attgctttca
      661 attgtttata aaataacgga gaggacaagt taaataatag aaattttgaa ataatctctg
      721 aactttcgaa tggtgttaat tggaaaataa atccaccagc cacaacacca tcatagtctt
      781 tatcgattct tgcaaacttt ccaatatgtt ctaaagaggt tgatacaggt gttattagct
      841 gattatgttt taaataaact tgctcagagg agatgaattg tgtatcaatg tagtaatcat
      901 tatccaacag agaaaattct aaaggcttaa tattaccacc acgtataatt ctaacacctt
      961 tattattaat gcttaaatcg cccttcttgt aagaaagacc tgtatttatt gaaaaaatat
     1021 cttttatttt tataacaacc caattcatag gtat
//
LOCUS       DAVBCN010000146         1042 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVBCN010000146 DAVBCN010000000
VERSION     DAVBCN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.146"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(<1..990)
                     /gene="ltrA"
                     /locus_tag="VQ497_002048"
     CDS             complement(<1..990)
                     /gene="ltrA"
                     /locus_tag="VQ497_002048"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HET2518309.1"
                     /translation="MSKLLDKILSRDNMLEAYNQVKSNKGSAGIDGITIEEMDGYLRQ
                     NWRLTKELIKQRKYKPQPVLRVEIPKPNGGIRQLGIPTVMDRMIQQAIVQVISPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAAAKRVMYSVSRFIEKRLGLKVNMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVWRFKLKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSL"
BASE COUNT          272 a          226 c          186 g          358 t
ORIGIN      
        1 caatgagaaa tagtttatcc atcctcgaat agacaaattc agttgctcaa tacgtcttgt
       61 taggtctata ctccatttcc tctgtgttag tttcttcaat ttaagcttaa atctccaaac
      121 actatcttga tgtggacgac ttttccaacc atctgatgat ttccagaacc cgaaacctag
      181 atatttcaac tctcttggtc tggtaatctt agtcttggtc atgtttactt tcagacctag
      241 ccgtttctca ataaaacgac tgactgaata catcacacgc ttagcggctg cctcgcttcc
      301 gaccgtaatc acacaatcgt ctgcgtagcg aacaaatcga agccctctct tttctaattc
      361 cttgtccaat tcattaagca tgatattgga taagagagga gataaatttc ctccctgtgg
      421 cgtgccaact agcgttttat aacgttgacc gttaatgata acacctgaat gaagatattt
      481 acgaatcaag gattccgtat ctccgtcttc gataatgtta tgtactaagg acatcaatct
      541 atcttgagga actgtatcga aaaatttctc taggtctatg tccactatcc actcatagcc
      601 gtcatttaag tactctaaga gcttcatgat ggctttttca catgacctat ttggtctgaa
      661 tccatagctc gtatctgaga aatggggttc acaaataggg ctaataactt gaacaatggc
      721 ctgttgaatc attctatcca taactgttgg aattcctagt tggcggatgc ctccgtttgg
      781 tttgggaatc tcaactctaa gaactggttg aggcttatat tttctctgtt ttatcagttc
      841 cttagtcagg cgccagtttt gtctgagata gccatccatc tcttcgatag taattccatc
      901 aatcccagct gagcctttat tagattttac ttgattgtag gcttcaagca tattgtcgcg
      961 tgataatatt ttatctagca attttgacat gtgcctattc ctttcttgtt ttggtgcctg
     1021 agggacatct tatattggtg aa
//
LOCUS       DAVBCN010000147         1032 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVBCN010000147 DAVBCN010000000
VERSION     DAVBCN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.147"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(741..1001)
                     /locus_tag="VQ497_002049"
     CDS             complement(741..1001)
                     /locus_tag="VQ497_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518310.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDITRLTASLLGDEKLSAITSSSA
                     VKADIHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
BASE COUNT          342 a          196 c          166 g          328 t
ORIGIN      
        1 tggtttcgca ggaagtctat tatatatgca cagacatatg aaatcttttt gagacagcag
       61 ttatgatata tttatccatt cacaacatct tctgtactaa tactacaata ttggtacatt
      121 ttgattacac aatcgtttca aaaaatatga tggaagcctg agtattttat agtaattccc
      181 ttcctatcaa ctagatcaac cagttgagtg ttgaatgttg aaacacccta gcctcagcaa
      241 aggactatga gcagcatttc ctagccaata ggacgctcca aatcacctat tctaaatcca
      301 tcactagctt ggtcagtaag atacagactg caaggcgaga ctgacgtgtt tgatgagatt
      361 ttggaagagt attacaatca tagattcacc agtagacttg accgcagatt taactatagt
      421 agattgaaac tagaatagta catctctgct tctaaaacat tgttagaaat cgatttgact
      481 gtcctgatca atttgtcctg ttctcgtttc atttcactat actaaaaaac cacctgaaac
      541 aaatcaagtg actagaggat aaaaacaaag actaggcaaa aagttttgaa aagcaaaaaa
      601 cgcataatat caggtcttga aaaaccttga tactatacgt ttgattgtgg gaagattgac
      661 ttcattttct cctgaaattg aagttttttc tagtttccag atatagacga attcaatata
      721 gccatctcgc tacaattatt ttaaccatcg agaaaatttt tcagaatttc aaaaatttca
      781 ttttgatcca atgaaatgtc atcattttga agaatagttg acttcacagg ctctaattct
      841 atcacaaagc ggtgtatgtc tgcttttacc gcactacttg atgttatagc cgataacttt
      901 tcatctccta gcaaggaagc tgtcaaacgg gtaatgtcat tcaaatgctt tttaatggac
      961 ttacttaaac cttgagcacc tgtagcagag cgttccctca tatccagcca agctttagcc
     1021 ttaaaaataa tt
//
LOCUS       DAVBCN010000148         1025 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVBCN010000148 DAVBCN010000000
VERSION     DAVBCN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.148"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            361..525
                     /locus_tag="VQ497_002050"
     CDS             361..525
                     /locus_tag="VQ497_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518311.1"
                     /translation="MKKKILIIFVLYLIMSIFLYPLRESAWYQLFYTIAYVIAVMIYF
                     AINKKKGEKK"
     gene            522..926
                     /locus_tag="VQ497_002051"
     CDS             522..926
                     /locus_tag="VQ497_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HET2518312.1"
                     /translation="MKTFFAKKRNIFLARLFLGQLPLLVSTYLFLSRQFLNFSVVFQF
                     LLVVINLASILVTVYLTREMRIREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITA
                     GESYQQIIAYIGAVLCLIIMLLLMWGLKYYKK"
BASE COUNT          326 a          150 c          176 g          373 t
ORIGIN      
        1 ataatttaat tagaaaaata ttttcaaaat ttcccaaaaa ggatacagat agtaccccct
       61 aaaaaataaa tttctcccct gtatttgcta gcttaattta caggggagtt tctttaattt
      121 tgtttcgcct gtcaaaatga tatgtgatag caggatgaaa agtctacaaa caagttggta
      181 tcaatacaat ataaagagga aagtatgttt ttaatgctta ctttttaaat tttttaacaa
      241 ttcaagatgt ttcgatgacc atttatgatt tgagtgatcc aaggtcaaaa taagtgctat
      301 actagcagtg taaatgttct tgctcaacta agataaatcg cacatttagg aggaaatctt
      361 atgaagaaaa aaatactgat tattttcgtt ctgtatctaa tcatgtccat ctttctatat
      421 ccgcttaggg agagtgcttg gtatcagcta ttttatacca tagcctatgt gattgcagtt
      481 atgatctatt ttgctatcaa taaaaagaaa ggagaaaaga aatgaaaact ttttttgcta
      541 aaaaacggaa catcttcctt gcgagattgt tcttaggtca gttgcccttg cttgtttcta
      601 cttatctatt tctatctcgt cagtttttaa atttttccgt agttttccaa tttcttttag
      661 tagttattaa cttggcttct attttggtta ctgtttatct cactagggaa atgaggataa
      721 gagagtttga agatgatgat ttggttagtc ctagaaccaa tcaacttatg ttcatcggct
      781 tgacaggttt tatgtctatt atttgtttgt atagaggtat cacagcagga gaatcctacc
      841 aacaaataat cgcttatatt ggcgctgttc tctgcttaat catcatgctt ctgctcatgt
      901 ggggcttgaa gtattataaa aagtagagct taatgaagta taaaagaaaa gggtaagtgt
      961 ttttgcaata cttacccttt tataatagat tccctgcgaa acaaaatatg gtatagtata
     1021 gtata
//
LOCUS       DAVBCN010000149          906 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVBCN010000149 DAVBCN010000000
VERSION     DAVBCN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.149"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            122..590
                     /locus_tag="VQ497_002052"
                     /pseudo
     CDS             122..590
                     /locus_tag="VQ497_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          332 a          136 c          167 g          271 t
ORIGIN      
        1 gagaagagga ttgccatctt ctccattttt atgtgcagag ttatagtggc ttgatgctgg
       61 gaaagtacac tgtgactgct aaaacattcc tagaagctgc tttgatttcc ctaatctatt
      121 tatgcaaatt ttatgttatt ttactataac agttgttgct aagccaaata atagtgggga
      181 agttcattta gacgtaagca ttgaagataa tcagggaggt agtgggtata atttcagttc
      241 tgtttcaagt agctcacaaa cagctaaata tgaaggaact ggttataata acaattcatc
      301 attatatata acgattgata aaacgtctga tgcaacagct cttttgaaat taaagttgaa
      361 taatgttgat aatcaacctg ctactgaagt tcctagttca ggaattactg taaaattaaa
      421 tgctaaagat aatgctggaa actggacaag tgcttcgaat aaagacaagt gcttcgaata
      481 aaaaagaaat aacagtaaaa attgtttctg ctaaaccgac atatccagac aaaatcttag
      541 tgaaaaatcc tgataatata aaagatacag aaaaaaatgc cattattgaa aaattgaaag
      601 aggcaaataa aaatcatcca gcaggagctc caacctttgc taaaggtgaa ggagagcatg
      661 caaatgatat tgtagcaact tattcagatg gtacaactta ttatgtaccg ttaaatgatg
      721 tgacaaaata tgcgaggtag tggctgtacc actcacttat tcacctcccc gtgatttgta
      781 gtagtgatag gctttctcac tattattata aaacaaaata aagatcacaa cactttttca
      841 ttctgtgttg tgccttgagt gaaacgaaag gaatgaatta taaatatgaa aagtatagtc
      901 actagc
//
LOCUS       DAVBCN010000150          872 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVBCN010000150 DAVBCN010000000
VERSION     DAVBCN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.150"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(697..>872)
                     /locus_tag="VQ497_002053"
     CDS             complement(697..>872)
                     /locus_tag="VQ497_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2518313.1"
                     /translation="NALQLPYSNAKLEATNNLIKLIKHNAFGFRNFENFKKRIFIALN
                     IKKERTKFVLSRS"
BASE COUNT          223 a          193 c          177 g          279 t
ORIGIN      
        1 cagagtccag ttctatcaga ccgttcgttt tacagacaca agtgaagtag gattacttcg
       61 attagttgtt taccccttcg ctccacttcc attacagaag tttcctcact actatgggct
      121 cggctgactt ctcatgattc gttgttacta cgctttcggc gctcatgaga cctcacggga
      181 taagccgtac atctttcctc gtttatacaa gatatgagga cgttaaaagg ttacagtgaa
      241 aataggaaat ctgacgcaga gtctttagac tctaggaaga tttatctttt tcacatagac
      301 cttagtccga attcaattac tctcgtgatt tacgcatata ggttacgact acctttttgg
      361 actttagagt tttaagcccc cttatccgct gtatacgcct tactatcacg ttcctgttcg
      421 tagagcctcg atttcgctat cccttcctct gacgattctt atttcctgcg tcagacttaa
      481 aacggtctat ccccagaccg ttttaatccg ctacctcacg atagtcaggc ttgggaatcg
      541 ctattgggtt caccggtagc aggtgcccac ggaggactta cacctcagat gcacgacatg
      601 cccgtcgtac ttaaaatcag cctaaatgat tcaggctgat ttttctattg attcaaaaaa
      661 acggctcttt gtcaactgta gtgggttgaa gaaaagctaa gatcgagaaa ggacaaattt
      721 cgtcctttct tttttgatat tcagagcgat aaaaatccgt tttttgaagt tttcaaagtt
      781 ccgaaaacca aaggcattgt gcttgataag tttgatgaga ttattggtcg cttccaattt
      841 ggcgtttgaa tagggtagtt gaagggcgtt ga
//
LOCUS       DAVBCN010000151          834 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVBCN010000151 DAVBCN010000000
VERSION     DAVBCN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.151"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(<1..>834)
                     /locus_tag="VQ497_002054"
     rRNA            complement(<1..>834)
                     /locus_tag="VQ497_002054"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          178 a          267 c          176 g          213 t
ORIGIN      
        1 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
       61 gcaccacctg tcacctctgt cccgaaggaa aactctatct ctagagcggt cagagggatg
      121 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      181 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      241 cttaatgcgt tagctacggc actaaacccc ggaaagggtc taacacctag cactcatcgt
      301 ttacagcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      361 cgtcagttac aagccagaga gccgctttcg ccaccggtgt tcctccatat atctacgcat
      421 ttcaccgcta cacatggaat tccactctcc cctcttgcac tcaagttaaa cagtttccaa
      481 agcctactat ggttaagcca cagcctttaa cttcagactt atctaaccgc ctgcgctcgc
      541 tttacgccca ataaatccgg acaacgctcg ggacctacgt attaccgcgg ctgctggcac
      601 gtagttagcc gtccctttct ggtaagatac cgtcacagtg tgaactttcc actctcacac
      661 tcgttcttct cttacaacag agctttacga tccgaaaacc ttcttcactc acgcggcgtt
      721 gctcggtcag acttccgtcc attgccgaag attccctact gctgcctccc gtaggagtct
      781 gggccgtgtc tcagtcccag tgtggccgat caccctctca ggtcggctat gtat
//
LOCUS       DAVBCN010000152          800 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVBCN010000152 DAVBCN010000000
VERSION     DAVBCN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.152"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(373..684)
                     /locus_tag="VQ497_002055"
     CDS             complement(373..684)
                     /locus_tag="VQ497_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518314.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYVCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
BASE COUNT          255 a          156 c          131 g          258 t
ORIGIN      
        1 tcaacggctg acattgaaaa ttcttctgga atctcaccct gcaaactctt acgttctttc
       61 ccaaatggca tatcgtaaag gtcgtatttc caacggcatg actgagaaca tccaccacgg
      121 ttggcatcac gcatactcta gacgcaattc taatctttta gactttgcat aataactgtt
      181 aaacagttga aaattaaccc agttttaaca agtagtcaaa ataccagata aaggaaaaaa
      241 agtctttaaa atcttaaaaa cgcatagtat caggtattga acaatcttga tattatgcgt
      301 tttattgtgg gaagatttac ttcattttct cttggaattg agttttctcc tacttcaaaa
      361 ctagatgatt tgctatttaa aatagcgttc cttatgattt tggcaacctg gattaaagcg
      421 agaaaaacaa aaggggcaac ttaagctttc tttatattgc ttatatgtta gtagttttag
      481 acaaacacca cataaaatag gcttatcctg tatcaaagat aagggatacg gctcaaaagg
      541 gtgattttct aatgaatcat gacaccgata acaagcatag tactttttac aaacatagca
      601 ttgtaaagca ataatatcca gcttgctatg gtaatgaatg catcttgttt gcttatccac
      661 taagaaaccc tgtgcctcta tcatttcctt ttcctttctt aagctcgaac tgtgacgctg
      721 gttccatctt ccttataaag attgatgagt ccttctttta atgcacgaac catgtctcca
      781 gcttgaacag gttgaggcac
//
LOCUS       DAVBCN010000153          707 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVBCN010000153 DAVBCN010000000
VERSION     DAVBCN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.153"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..71
                     /locus_tag="VQ497_002056"
     rRNA            <1..71
                     /locus_tag="VQ497_002056"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            123..195
                     /locus_tag="VQ497_002057"
     tRNA            123..195
                     /locus_tag="VQ497_002057"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:156..158,aa:Ala,seq:tgc)
     gene            320..>707
                     /locus_tag="VQ497_002058"
     rRNA            320..>707
                     /locus_tag="VQ497_002058"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          218 a          122 c          205 g          162 t
ORIGIN      
        1 tgggatagat gattggggtg aagtcgtaac aaggtagccg tatcggaagg tgcggctgga
       61 tcacctcctt tctaaggata aggaactgcg cattggtctt gtttagtctt gagaggtctt
      121 gtggggcctt agctcagctg ggagagcgcc tgctttgcac gcaggaggtc agcggttcga
      181 tcccgctagg ctccattggt gagagatcac caagtaatgc acattgaaaa ttgaatatct
      241 atatcaaata gtaacaagaa aataaaccga aaacgctgta gtattaataa gagtttatga
      301 ctgaaaggtc agaaaataag gttaagttaa taagggcgca cggtggatgc cttggcacta
      361 ggagccgacg aaggacgtga caaacgacga tatgccttgg gtagctgtaa gtaagcgatg
      421 atccagggat ttccgaatgg gggaacccaa caggtagtac ctgttaccca catctgttaa
      481 ggatgtgagg aggaagacgc agtgaactga aacatctaag tagctgcagg aagagaaagc
      541 aaaagcgatt gccttagtag cggcgagcga aacggcagga gggcaaaccg aagagtttac
      601 tcttcggggt tgtaggactg caatgtggac tcaaagatta tagaagaatg atttgggaag
      661 atcagccaaa gagagtaata gcctcgtatt taaaatagtc tttgtac
//
LOCUS       DAVBCN010000154          692 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVBCN010000154 DAVBCN010000000
VERSION     DAVBCN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.154"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(74..147)
                     /locus_tag="VQ497_002059"
     tRNA            complement(74..147)
                     /locus_tag="VQ497_002059"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Arg,seq:acg)
     gene            complement(157..242)
                     /locus_tag="VQ497_002060"
     tRNA            complement(157..242)
                     /locus_tag="VQ497_002060"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(206..208),aa:Leu,seq:taa)
     gene            complement(250..321)
                     /locus_tag="VQ497_002061"
     tRNA            complement(250..321)
                     /locus_tag="VQ497_002061"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Gly,seq:gcc)
     gene            complement(344..416)
                     /locus_tag="VQ497_002062"
     tRNA            complement(344..416)
                     /locus_tag="VQ497_002062"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(381..383),aa:Thr,seq:tgt)
     gene            complement(435..516)
                     /locus_tag="VQ497_002063"
     tRNA            complement(435..516)
                     /locus_tag="VQ497_002063"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(480..482),aa:Leu,seq:tag)
     gene            complement(522..594)
                     /locus_tag="VQ497_002064"
     tRNA            complement(522..594)
                     /locus_tag="VQ497_002064"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(559..561),aa:Lys,seq:ttt)
     gene            complement(619..691)
                     /locus_tag="VQ497_002065"
     tRNA            complement(619..691)
                     /locus_tag="VQ497_002065"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(656..658),aa:Asp,seq:gtc)
BASE COUNT          179 a          205 c          149 g          159 t
ORIGIN      
        1 cgaacctgcg acaccttggt cccaaaccaa gtactctacc aagctgagct acttcccgag
       61 ttaaatagaa aaatgcaccc tagaggagtc gaacctctaa ccgcctgatt cgtagtcagg
      121 tactctatcc agttgagcta agggtgctcc atattatgcc gaggaccgga atcgaaccgg
      181 tacgatcgtt accaatcgca ggattttaag tcctgtgcgt ctgccagttc cgccaccccg
      241 gcctctctaa gcgaacgacg ggattcgaac ccgcgacccc caccttggca aggtggtgtt
      301 ctaccactga actacgttcg cactgttttc ttctatctaa aaatgccggc tacatgactt
      361 gaacacgcga ccctctgatt acaaatcaga tgctctacca actgagctaa gccggctcat
      421 ttgttatatc ttaatgcggg ttaagggact tgaaccccca cgccgttaag cgccagatcc
      481 taaatctggt gcgtctgcca attccgccaa acccgcatat atgacccgta ctgggctcga
      541 accagtgacc cattgattaa aagtcaattg ctctaccaac tgagctaacg agtctaaaat
      601 aacttgcgtt accttaaacg gtcccgacgg gaatcgaacc cgcgatcttc gccgtgacag
      661 ggcgacgtga taaccgctac actacgggac ct
//
LOCUS       DAVBCN010000155          685 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVBCN010000155 DAVBCN010000000
VERSION     DAVBCN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.155"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(1..175)
                     /locus_tag="VQ497_002066"
                     /pseudo
     CDS             complement(1..175)
                     /locus_tag="VQ497_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433508.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(270..455)
                     /locus_tag="VQ497_002067"
                     /pseudo
     CDS             complement(270..455)
                     /locus_tag="VQ497_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            459..685
                     /locus_tag="VQ497_002068"
                     /pseudo
     CDS             459..685
                     /locus_tag="VQ497_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927991.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          200 a          122 c          135 g          228 t
ORIGIN      
        1 ttgacaatct tctctttatc tttgaggaag gttttagagg atgaacctga ttcagattgt
       61 cctcaatgag tccgaaaaat ttgtcaggct ccttattctg aaagtgaaaa agcaagagtt
      121 gatagagatt atagtggtgt ttcaagtctt ctgaatagct caaaagttta tctatagtag
      181 attgaaacta gaatagtaca cctctgcttc taaaacattg ttagaaatcg atttgactgt
      241 cctgaatgat ttgtcctgtt attatttcat tttactataa atccacgttt acgaatctct
      301 ttccacactt gttcaatggg gttcatctct ggtgtgtatg gaggaataaa tgcaaaacca
      361 atattagtcg gaatctttaa ggtacttgat ttatgccata tagcattgtc cataacgagt
      421 aaaagataat catctggata agcttgtgaa agctcctatt cctaaagccc ctttataacc
      481 tcttgcgaga gagactattg actcagccct tacttcatgc ggatgaaact tcttatcggg
      541 ttctagagag tcatagccat ctgacctact attggacctt tttgtctggg aaagttgaga
      601 atcaagcaat cacgctgtac catcatgatc agagtcggag tggttcggta gtacaagaat
      661 tcctaggaga ttattctggc tatgt
//
LOCUS       DAVBCN010000156          671 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVBCN010000156 DAVBCN010000000
VERSION     DAVBCN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.156"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..>671
                     /locus_tag="VQ497_002069"
     CDS             <1..>671
                     /locus_tag="VQ497_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2518315.1"
                     /translation="HVESDGLVFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEKR
                     IARIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSLQPAPNPQPAPSNPIDEKLVKEAV
                     RKVGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDRE
                     FYNKTYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKLVDDILAFLAPIR
                     HPERLG"
BASE COUNT          210 a          148 c          137 g          176 t
ORIGIN      
        1 catgtagaat ctgatggcct tgttttcgac ccagcgcaaa tcacaagtcg aaccgccaga
       61 ggtgtagctg tccctcatgg taaccattac cactttatcc cttatgaaca aatgtctgaa
      121 ttggaaaaac gaattgctcg tattattccc cttcgttatc gttcaaacca ttgggtacca
      181 gattcaagac cagaacaacc aagtccacaa tcgactccgg aacctagtcc aagtctgcaa
      241 cctgcaccaa atcctcaacc agctccaagc aatccaattg atgagaaatt ggtcaaagaa
      301 gctgttcgaa aagtaggcga tggttatgtc tttgaggaga atggagtttc tcgttatatc
      361 ccagccaagg atctttcagc agaaacagca gcaggcattg atagcaaact ggccaagcag
      421 gaaagtttat cgcataagct aggagctaag aaaactgacc tcccatctag tgatagagaa
      481 ttttacaata agacttatga cttactagca agaattcacc aagatttact tgataataaa
      541 ggtcgacaag ttgattttga ggctttggat aacctgttgg aacgactcaa ggatgtctca
      601 agtgataaag tcaagttagt ggatgatatt cttgccttct tagctccgat tcgtcatcca
      661 gaacgtttag g
//
LOCUS       DAVBCN010000157          663 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVBCN010000157 DAVBCN010000000
VERSION     DAVBCN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.157"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            91..>663
                     /locus_tag="VQ497_002070"
     CDS             91..>663
                     /locus_tag="VQ497_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HET2518316.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNY"
BASE COUNT          249 a          114 c          135 g          165 t
ORIGIN      
        1 ctgttgtcaa atgaaatgtg atttaaggta taagaaaaca cctctgtgct atacttgttg
       61 ttcaccacaa acacaaggaa aggcacagag atgcaagaac attatacccc aaaagggaaa
      121 catttgacaa tagataaccg tcgcttgatt gagcggtgga agaatgaaaa taagtccaat
      181 cgtgaaattg caggcttgtt aggaaaggcg cctcaaacga ttcataatga agtcaaaaga
      241 ggtacaactt tacaacaagt gagaaaaggg ctatacaaaa aggtctattc tgccgattac
      301 gcacaaactg tttaccaatt caatcgaaaa cggtcggtga aaaagttaat tttaacaaag
      361 gaaatcagag agaagatctt acactatcat aagcaaaaat tttcgcctga aatgatggtt
      421 aacaagaagc aagtgaaagt tggtatttca accatctact actggtttca taatggtcat
      481 ttaggattga cgaaggccga catgctttat cccagaaaaa ggaaaggtgt caagaagcaa
      541 gctagtccga actttaagcc ggcaggtaaa tctatcgaag aacgtcctga cgttattaat
      601 cttcgcttgg aaaatggtca ttatgaaatt gataccgtcc tactgactaa gataaaaaat
      661 tat
//
LOCUS       DAVBCN010000158          636 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVBCN010000158 DAVBCN010000000
VERSION     DAVBCN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.158"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..>636
                     /locus_tag="VQ497_002071"
     rRNA            <1..>636
                     /locus_tag="VQ497_002071"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          179 a          135 c          181 g          141 t
ORIGIN      
        1 agcgaagttt agtagcgaag ttagtgacgt cacactgcca agaaaagctt ctagcgttta
       61 aacatactct acccgtaccg caaaccgaca caggtagtcg aggcgagtag cctcaggtga
      121 gcgagagaac tctcgttaag gaactcggca aaatgacccc gtaacttcgg gagaaggggt
      181 gctgacttta agtcagccgc agtgaatagg cccaagcaac tgtttatcaa aaacacagct
      241 ctctgctaaa tcgtaagatg atgtataggg ggtgacgcct gcccggtgct ggaaggttaa
      301 gaggagtgct tagcgtaagc gaaggtatga attgaagccc cagtaaacgg cggccgtaac
      361 tataacggtc ctaaggtagc gaaattcctt gtcgggtaag ttccgacccg cacgaaaggc
      421 gtaatgattt gggcactgtc tcaacgagag actcggtgaa attttagtac ctgtgaagat
      481 gcaggttacc cgcgacagga cggaaagacc ccatggagct ttactgcagt ttgatattga
      541 gtgtctgtac cacatgtaca ggataggtag gagtctaaga gatcgggacg ccagtttcga
      601 aggagacgct gttgggatac tacccttgtg ttatgg
//
LOCUS       DAVBCN010000159          616 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVBCN010000159 DAVBCN010000000
VERSION     DAVBCN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.159"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(194..>616)
                     /locus_tag="VQ497_002072"
     CDS             complement(194..>616)
                     /locus_tag="VQ497_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET2518317.1"
                     /translation="KPNEEKPQTEKPEEDKEHDEVSEPTHPESDEKENHAGLNPSADN
                     LYKPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIAEAEALLEKVTDSSIRQNAVET
                     LTGLKSSLLLGTKDNNTISAEVDSLLALLKESQPTPIQ"
BASE COUNT          158 a          121 c           93 g          244 t
ORIGIN      
        1 taaatgatga agttttttct caacttcttt tactttatta aatagttttc taaaccggtt
       61 atttaaccat ttaattaacc agtaaatagt ttactattta aaattacttt tgtcaagttt
      121 ctgttacgtt tttctaaaaa agagaagtaa cacgaaaggt tacttctcca taaaatatgc
      181 tccattcatt ttactactgt ataggagtcg gttgactttc ttttaacaaa gccaagagac
      241 tatctacttc tgctgaaata gtgttattat ctttcgttcc gagaagaaga ctacttttta
      301 gaccagtcaa tgtctccaca gcattttgtc taatactaga atctgttact ttttctagca
      361 aggcctccgc ctctgctatc ttagcgttaa taacagaatg ctctacttga ggaatttcag
      421 cctcatctgt agtatcttca gcttcttcct ctgtctcttc cgtatcagtg cttggtttat
      481 aaagattatc tgctgaagga tttaaaccag cgtgattctc tttttcatca gattcagggt
      541 gagttggctc acttacttca tcatgttcct tatcttcctc aggtttttct gtctgaggtt
      601 tctcctcgtt tggttt
//
LOCUS       DAVBCN010000160          615 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVBCN010000160 DAVBCN010000000
VERSION     DAVBCN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.160"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            48..463
                     /locus_tag="VQ497_002073"
                     /pseudo
     CDS             48..463
                     /locus_tag="VQ497_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433508.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(498..>615)
                     /locus_tag="VQ497_002074"
     CDS             complement(498..>615)
                     /locus_tag="VQ497_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET2518318.1"
                     /translation="PEYNPIEKTWAYIKKNLKKVLPSCNTFYEALFSCSCFN"
BASE COUNT          209 a          137 c           91 g          178 t
ORIGIN      
        1 ctcaatatca taactgttct tgagagcaga acacaagcca tcatccgaaa tcccatgaat
       61 acaaggctat caagcgctac tggaagctca ttcaacaaga tagtcagaaa ctgagtgata
      121 aacgatttta tcgccctact tttcgtatgc acttgactaa taaagaaatt ctagataaac
      181 ttttgagcta ttccgaagac ttgaaacacc actataatct ctatcagctc ttgcttttcc
      241 acctctgttt cagactttgt ttaaaacctt cctcaaagat aaagagaaaa tcgtcaacgc
      301 ccttcaatta ccttattcca acgccaaact ggaagccacc aataatctca tcaaacttat
      361 caagcacaat gcctttggtt ttaggaactt tgaaaacttc aaaaaagaaa ggacgaaatt
      421 tgtcctttct agatcttcgc tttcttcaac ccactacagt tgacaaagaa ccgaaaaatg
      481 tctcgcctct aatatagtca attgaaacaa gaacaagaga aaagagcctc gtaaaaggta
      541 ttgcaacttg gtaatacctt tttgaggttc tttttgatat aagcccatgt tttctcaata
      601 ggattgtact caggt
//
LOCUS       DAVBCN010000161          602 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVBCN010000161 DAVBCN010000000
VERSION     DAVBCN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.161"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            40..>602
                     /locus_tag="VQ497_002075"
     CDS             40..>602
                     /locus_tag="VQ497_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518319.1"
                     /translation="MLAEADALVDADSEALVDAETEALVDADSDALVLAEAEALVDAE
                     AEALVDAEADALVDADSDAEVLAEAEALVDAEAEALVDAETEALVLAEAEALVLTEAE
                     ALVLAEAEALVDADSEALVDAETEALVDAEADALVLAEAEALVDADSEADVLAEAEAL
                     VLAEADALVDAEAEALVLAEAEALVLA"
BASE COUNT          125 a          136 c          197 g          144 t
ORIGIN      
        1 gaagccgacg cactggtgct tgctgaagcc gaggcgctag tgcttgctga agccgacgca
       61 ctcgttgatg cggattcaga ggcactggtt gacgctgaga ctgaagcact cgttgatgct
      121 gactcagacg cactggtact tgcggaagcc gaagcactgg ttgatgctga agccgaggcg
      181 cttgtcgatg cggaagctga tgcacttgtt gatgccgatt cagacgctga ggtgcttgct
      241 gaagctgagg cgctcgttga cgctgaagcc gaagcactgg ttgatgctga aactgaggca
      301 cttgtgcttg ctgaagccga agcactggta cttactgaag ctgaggcact cgtacttgct
      361 gaagccgagg cacttgttga tgcggattca gaggcactgg ttgacgctga gactgaagca
      421 ctcgttgatg ctgaagccga cgcactggta cttgctgaag ccgaggcact tgttgatgcg
      481 gattcagaag ctgatgtgct tgctgaagct gaggcgctag tacttgctga agctgatgca
      541 ctggttgatg ctgaggctga agcacttgta cttgctgagg ctgaggcgct tgtacttgct
      601 ga
//
LOCUS       DAVBCN010000162          587 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVBCN010000162 DAVBCN010000000
VERSION     DAVBCN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.162"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..>587
                     /locus_tag="VQ497_002076"
     rRNA            <1..>587
                     /locus_tag="VQ497_002076"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          144 a          134 c          183 g          126 t
ORIGIN      
        1 gacgggcagt ttgactgggg cggtcgcctc ctaaaaggta acggaggcgc ccaaaggttc
       61 cctcagaatg gttggaaatc attcgcagag tgtaaaggta taagggagct tgactgcgag
      121 agctacaact cgagcaggga cgaaagtcgg gcttagtgat ccggtggttc cgtatggaag
      181 ggccatcgct caacggataa aagctaccct ggggataaca ggcttatctc ccccaagagt
      241 tcacatcgac ggggaggttt ggcacctcga tgtcggctcg tcgcatcctg gggctgtagt
      301 cggtcccaag ggttgggctg ttcgcccatt aaagcggcac gcgagctggg ttcagaacgt
      361 cgtgagacag ttcggtccct atccgtcgcg ggcgtaggaa atttgagagg atctgctcct
      421 agtacgagag gaccagagtg gacttaccgc tggtgtacca gttgtcttgc caaaggcatc
      481 gctgggtagc tatgtaggga agggataaac gctgaaagca tctaagtgtg aaacccacct
      541 caagatgaga tttcccatga ttatatatca gtaagagccc tgagaga
//
LOCUS       DAVBCN010000163          585 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVBCN010000163 DAVBCN010000000
VERSION     DAVBCN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.163"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
BASE COUNT          182 a          104 c          155 g          144 t
ORIGIN      
        1 gaggtgtaag tcctcggtgg gcacctgcta ccggtgaacc caatagcgat tcccaagcct
       61 gactatcgtg aggtagcagg gagaggaagg gatagcgaaa tcgtggctct acgaacagga
      121 acgtgatagt aaggcgtata tagcggataa ggaggcttca aactctaaag tccaaaaagg
      181 tagtcgtaac ctatatgtgt aaatcacgag agtaattgaa ttcggactaa ggtttgtgtg
      241 aaaaagataa atctttctag agtctaaaga ctctgcgtca gatttcctat tttcactgta
      301 accttttaac gtcctcatat cttgtataaa cgaggaaaga tgtacgactt atcccgtgag
      361 gtttcatgag cgctgaaagc gtagtaacaa cgaatcatga gaagtcagcc gagcccatag
      421 tagtgaggaa acttccgtaa tggaagtgga gcgaaggggt gaatactcaa acagtctggg
      481 gagagactgt ttgaggtctg tcgctagaaa gagaaaacga cagatcgaag taatcctact
      541 tcacttgtgt ctgtaaaatg agtggtctga tagaactgga ctttg
//
LOCUS       DAVBCN010000164          572 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVBCN010000164 DAVBCN010000000
VERSION     DAVBCN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.164"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            212..427
                     /locus_tag="VQ497_002077"
     CDS             212..427
                     /locus_tag="VQ497_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518320.1"
                     /translation="MILWSFDFANDHAHAFFMDNVEWSHADSYFLSFVSDDVEERYIE
                     NVYLDSLSVKQKFKFIFDFGDEWRFEC"
BASE COUNT          190 a           83 c          127 g          172 t
ORIGIN      
        1 aaaacaagaa caccttgctt ggcgcagggt gtttcgcgtg aaacagaaga attatctggt
       61 ttcaaatgct acagttaaaa ttagtaaaac aagaaataga agctgaaaag ccagcatctg
      121 tagaagcttg gatcatttcc gtcaaattaa aaaaggttgc taccgacata tatagattcc
      181 aaaaacaaaa acgttagcgg aactagcaga tgtgatttta tggagtttcg attttgcaaa
      241 tgatcatgct catgcatttt tcatggataa tgttgagtgg agtcatgcag attcttactt
      301 tcttagcttt gttagtgacg atgttgaaga acgttacata gaaaatgtct atctggatag
      361 cctaagtgtc aaacaaaaat ttaagtttat tttcgacttc ggtgatgaat ggcgttttga
      421 atgctaagtg ctgagagaaa tcgagacaga ggacgaagaa gcttatctcg tacgttcggt
      481 tggaacgttg ccagaacaat atccagatta tgatggtttt gactatgaag aatggtaaaa
      541 ttgaaatcag tctgtgtagg cttagtattt ca
//
LOCUS       DAVBCN010000165          542 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVBCN010000165 DAVBCN010000000
VERSION     DAVBCN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.165"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..>542
                     /locus_tag="VQ497_002078"
     CDS             <1..>542
                     /locus_tag="VQ497_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2518321.1"
                     /translation="EQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEELLMKDPNY
                     QLKDEDIISEIKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHSQHREGGTS
                     TNDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNELSASELA
                     AAEAFLSGRGNLSNSRTYRR"
BASE COUNT          176 a          107 c          121 g          138 t
ORIGIN      
        1 gaacaaatcg tcatcaagat aacagaccaa ggctatgtca cttcacatgg ggaccactat
       61 cattattaca atggtaaggt tccttatgac gctatcatca gtgaagaatt gctgatgaag
      121 gatccaaact accagctaaa ggacgaggat attatcagtg aaatcaaggg tggttatgtg
      181 attaaggtag atggaaaata ctatgtttac cttaaggatg cagctcatgc ggataatgtc
      241 cgtacaaaag aagaaatcaa tcggcaaaaa caagaacata gtcagcatcg tgaaggaggg
      301 acttcaacaa acgatggtgc ggtagccttt gcacgttcac aaggacgcta tactacagat
      361 gatggttata tctttaatgc ttctgatatc attgaggaca cgggtgatgc ttatatcgtt
      421 cctcacggcg accattacca ttacattcct aagaatgagt tatcagctag cgagttagct
      481 gctgcagaag ccttcctatc tggtcgggga aatctgtcaa attcaagaac ctatcgccga
      541 ca
//
LOCUS       DAVBCN010000166          516 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVBCN010000166 DAVBCN010000000
VERSION     DAVBCN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.166"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            1..516
                     /locus_tag="VQ497_002079"
                     /pseudo
     CDS             1..516
                     /locus_tag="VQ497_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
BASE COUNT          158 a          101 c          118 g          139 t
ORIGIN      
        1 aacgatttgg cgaaacgtaa aaaaatatga ggagttcgga ctcgactctc tccttcaaga
       61 aacacgtggt ggtcgtaacc atgcatatat gacagttgag gaagagaaag cctttcttgc
      121 ccgtcatttg aaggctgcag aggcaggaga atttgttaca attgatgcct tatttcaggc
      181 ttataaaaag gagttaggtc gttcctacac acgtgatgcc ttctatcaac tgttgaagcg
      241 ccatggttgg cgaaatatta cgccacgtcc agaacatcct aagaaagcag acgctcaaac
      301 cattgtcgcg tctaaaaata aaatctcaat tcaagaagac aagaaagcgc tttaaaacca
      361 gtagaccttt tcataaggtt cgcttgatgt accaagatga ggctggtttc ggtagaatca
      421 gtaaactggg atcttgttgg tctccaatag gagtaggtcc acatgtccat agtcactata
      481 tacgagaatt tcgctattgt tatggagctg ttgatg
//
LOCUS       DAVBCN010000167          513 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVBCN010000167 DAVBCN010000000
VERSION     DAVBCN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.167"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            152..460
                     /locus_tag="VQ497_002080"
                     /pseudo
     CDS             152..460
                     /locus_tag="VQ497_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
BASE COUNT          204 a           64 c           97 g          148 t
ORIGIN      
        1 tatcgcttac caatatttgc taaactttca gaaaaaaagt tggagtatat gatgctaggt
       61 gactgcgtca tgttagtaaa cgaaatggaa ataactgatc atagagtaga taacttattt
      121 gaaaaaggga aaaatgagat aaaagattca agtagtagat ttagcaatag gacatataaa
      181 agctttagaa aaagtatctg aaaaaacaga tgtttatatc tataacctag gctcgggaga
      241 aggcactagt gtattacaac ttgtaaatac atttgaaagt gttaataaga tccctattcc
      301 ttataaaata gttccaagac gttcaggaga cgttgcaact tattatgcaa atgcagacaa
      361 agcatataag gaattaaatt ggaggacaac aaaatcgatt gaagacatgt gtagagatac
      421 atggaattgg caatcaaaaa atcccaatgg ctataattga tgatactgtc tatatgagtg
      481 ctgtattttg gaaaaattac ttaacctttt aat
//
LOCUS       DAVBCN010000168          482 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVBCN010000168 DAVBCN010000000
VERSION     DAVBCN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.168"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            30..>482
                     /locus_tag="VQ497_002081"
     CDS             30..>482
                     /locus_tag="VQ497_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET2518322.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVF"
BASE COUNT          178 a           62 c          116 g          126 t
ORIGIN      
        1 gttagactgt atctagaaag gggaaaatta tgattaaaga attgtatgaa gaagtccaag
       61 ggactgtgta taagtgtaga aatgaatatt accttcattt atgggaattg tcggattggg
      121 accaagaagg catgctctgc ttacatgaat tgattagtag agaagaagga ctggtagacg
      181 atattccacg tttaaggaaa tatttcaaaa ccaagtttcg aaatcgaatt ttagactata
      241 tccgtaagca ggaaagtcag aagcgtagat acgataaaga accctatgaa gaagtgggtg
      301 agatcagtca tcgtataagt gaggggggtc tctggctaga tgattattat ctctttcatg
      361 aaacactaag agattataga aacaaacaaa gtaaagagaa acaagaagaa ctagaacgcg
      421 tcttaagcaa tgaacgattt cgagggcgtc aaagagtatt aagagactta cgcattgtgt
      481 tt
//
LOCUS       DAVBCN010000169          455 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVBCN010000169 DAVBCN010000000
VERSION     DAVBCN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.169"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(<1..>455)
                     /locus_tag="VQ497_002082"
     CDS             complement(<1..>455)
                     /locus_tag="VQ497_002082"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2518323.1"
                     /translation="LVLAEADALVLAEADALVLAEADALVDADSDALVDADSDAEVLA
                     EADALVLAEADALVLAEAEALVLAEAEALVLADSDALVLAEAEALVEAEADALVLAET
                     EALVLAEAEALVDAEAEALVLAEAEALVDADSDAEVLAEADALVDAEAEA"
BASE COUNT          116 a          143 c          106 g           90 t
ORIGIN      
        1 gcctcagcct cagcatcaac cagtgcgtcg gcttcagcaa gtacctcagc gtctgaatcg
       61 gcatcaacaa gtgcttcagc ttcagcaagt acaagcgcct cagcttcagc atcaaccagt
      121 gcttcggctt cagcaagcac cagtgcctca gtctcagcaa gcaccagtgc gtcggcttca
      181 gcctcaacca gcgcctcagc ctcagcaagt acaagtgcat cagagtcagc aagtaccagt
      241 gcttcggctt cagcaagtac tagcgcctca gcttcagcaa gtaccagtgc gtcagcttca
      301 gcaagtacca gtgcgtcggc ctcagcaagt acctcagcgt ctgaatcggc atcaacgagt
      361 gcgtctgaat ccgcatcaac aagtgcgtca gcttcagcaa gtacaagtgc gtcagcttca
      421 gcaagtacaa gtgcgtcagc ttcagcaagt actag
//
LOCUS       DAVBCN010000170          438 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVBCN010000170 DAVBCN010000000
VERSION     DAVBCN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.170"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            30..>438
                     /locus_tag="VQ497_002083"
     CDS             30..>438
                     /locus_tag="VQ497_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2518324.1"
                     /translation="MNFLHEQGYKVYTYNPLLIKKFAKSLSLRKTKTDKKDAHGIALK
                     LLSDPNREQFQHNNRQVELKILTRHIHRLKKKQSDWKVQYTRCLDIIFPELDKIVGKH
                     SEYTYQLLTRYPNPQKRIEAGFDKLIEIKRLTAS"
BASE COUNT          155 a           95 c           69 g          119 t
ORIGIN      
        1 aagatactgg acattatgcc tttaatctac tgaacttcct gcatgaacaa ggatataaag
       61 tttacaccta caatcctttg ctcatcaaga aatttgccaa atcattatca cttcgcaaga
      121 ctaaaacgga taagaaagac gctcatggca tcgctcttaa acttctttct gaccccaatc
      181 gtgaacaatt tcaacacaat aatcggcaag tagaactaaa aatactgact agacatattc
      241 atcgtctcaa gaaaaaacag tctgattgga aagtacaata cactcgttgc cttgatatca
      301 tctttcctga gttggataaa atcgttggaa agcattcaga atatacctac caactcttga
      361 cacgctaccc taatcctcag aaaaggattg aggcaggatt tgataagctg atagaaatta
      421 agcgattgac cgcttcta
//
LOCUS       DAVBCN010000171          410 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVBCN010000171 DAVBCN010000000
VERSION     DAVBCN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.171"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..>410
                     /locus_tag="VQ497_002084"
     CDS             <1..>410
                     /locus_tag="VQ497_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5-like element IS1515 family transposase"
                     /protein_id="HET2518325.1"
                     /translation="GKKKRHTLKTQIMLDLTTHKVCQMAFSDGHTHDFTLFKESIGQS
                     LPETTLAFVDLGYLGILKFHENTFIPAKNSKNRRLSEDDKQLNKEMSAIRIEIEHFNA
                     KFKTFQIMSVPYRNRRKRFELRAELICAIINYEV"
BASE COUNT          138 a           78 c           76 g          118 t
ORIGIN      
        1 tggtaaaaag aaacgacaca ccttaaaaac tcaaattatg ctggatttga cgacacataa
       61 agtctgtcaa atggcctttt ctgacggaca tacgcatgat tttactctct tcaaagaaag
      121 tattggacaa agtttgcctg aaacgacgct tgcctttgtt gacctaggtt atttaggcat
      181 cttgaaattt catgagaata ctttcattcc tgctaaaaat tccaaaaatc gccgcctgag
      241 tgaggatgat aagcagttaa ataaagagat gtcagcgata cgaattgaaa ttgaacattt
      301 taacgctaaa ttcaagacct tccaaatcat gtcagtccct tatcgtaacc gcagaaaacg
      361 tttcgagtta cgggcggaat taatttgtgc catcatcaat tatgaagtga
//
LOCUS       DAVBCN010000172          402 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVBCN010000172 DAVBCN010000000
VERSION     DAVBCN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.172"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            90..365
                     /locus_tag="VQ497_002085"
     CDS             90..365
                     /locus_tag="VQ497_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2518326.1"
                     /translation="MFDKEQKRGYKQSARNLANWYYNDKEDSYTHPDGWYYRFHHTKH
                     QKTQTDFKQEIKVYYADEPESAPQKGLYMNEGYQNLKAKECHLSLGR"
BASE COUNT          143 a           74 c           85 g          100 t
ORIGIN      
        1 taatggacaa gttctcccaa aatcgttcag ccatattgct tctcctttag ttagatgaaa
       61 aggaggtaaa ccatctgatt aaatatgcca tgtttgataa ggaacagaag agaggctata
      121 aacagtcagc tagaaactta gcgaattggt actataatga caaggaggat agctatacac
      181 atcctgatgg ctggtattat cgttttcacc ataccaaaca tcagaaaaca cagacagact
      241 ttaaacaaga aatcaaggtt tactacgccg acgaacctga atcagcccct caaaagggac
      301 tgtatatgaa cgaaggctat caaaacttga aagctaaaga atgtcacctg tctttgggca
      361 gataaaggct tgtttgggtt acaagagatg taatctgagg gg
//
LOCUS       DAVBCN010000173          396 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVBCN010000173 DAVBCN010000000
VERSION     DAVBCN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.173"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(46..>396)
                     /locus_tag="VQ497_002086"
     CDS             complement(46..>396)
                     /locus_tag="VQ497_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2518327.1"
                     /translation="ASIIAHTIHQKFNLKVPNYRQEEDWARMGLPITRKEISNRHIKT
                     SQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYWTFLSGKAEKQGITLYH
                     HVLIDLFISYFNPL"
BASE COUNT          122 a           74 c           82 g          118 t
ORIGIN      
        1 cgtttggaag acattctaga agataggata gatatttctc actatttata atggattgaa
       61 ataagatatg aacaaatcga ttaggacatg atggtaaagc gtaatccctt gtttctcagc
      121 tttcccagac aaaaaagtcc aatagtaagt cagctgacta tcactctcta gcaccctata
      181 agaagtttca tccgcatgaa gtaagggctg agtcaatagt ctctctcgca agaggttata
      241 aaggggctcc aaatagtatt gactcgtctt gatatgccga ttagagattt ccttacgtgt
      301 gattggtaaa cccatcctag cccaatcttc ttcttggcga taattgggta ccttcagatt
      361 aaacttctga tggatggtgt gagcgataat agaagc
//
LOCUS       DAVBCN010000174          392 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVBCN010000174 DAVBCN010000000
VERSION     DAVBCN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.174"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            283..>392
                     /locus_tag="VQ497_002087"
     CDS             283..>392
                     /locus_tag="VQ497_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HET2518328.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVATLLEEREIV"
BASE COUNT          143 a           53 c           70 g          126 t
ORIGIN      
        1 ggttacgata tgatcactga cttcgtcagt cttatctaca acctcaaaac tgtgttttga
       61 gcaacctgcg gctagcttcc tagtttgctc tttgattttc attgagtatt agaacagaaa
      121 aaatacttgg agtatttgtt tgtgtgttta tttttatata acaaactata aacaaaacaa
      181 aaatataaaa aaagagacaa aaaagaacag aaagcaattg acaacggttt catatagtgt
      241 tatacttatg gcataaagtt aatggaaaga agggaaaaac ttatggtatt agattatttc
      301 tttgacaaaa accttgtgtt ttgcttagaa gcggataatc aagaacaact ctttgatcag
      361 gttgcaactt tattggaaga acgagaaatt gt
//
LOCUS       DAVBCN010000175          391 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVBCN010000175 DAVBCN010000000
VERSION     DAVBCN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.175"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..>391
                     /locus_tag="VQ497_002088"
     CDS             <1..>391
                     /locus_tag="VQ497_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2518329.1"
                     /translation="HCSKMMVAETPLVKKNHQIPRIINQKIAQKLIEKISMTDIAHQL
                     AISTSTVIRKLNDFHFEHDFSRLPKIMSWDEYAFTKGKMSFIAQDFDNLNIITVLEGR
                     TQAVIRNHFLRYDRAVRCQVKIITMDM"
BASE COUNT          121 a           80 c           68 g          122 t
ORIGIN      
        1 atcactgttc aaaaatgatg gttgctgaga ctcccctggt aaagaaaaat caccaaatcc
       61 ctcgtatcat caaccaaaaa attgctcaaa agttaattga aaaaatttct atgactgata
      121 ttgcccatca gctggccatt tcaacttcaa ctgtcattcg caagctcaat gactttcact
      181 ttgagcatga tttttctcgg cttccaaaga ttatgtcttg ggatgagtat gccttcacta
      241 agggaaagat gagtttcatt gcgcaagatt ttgataatct taatattatc actgttcttg
      301 aaggcagaac acaagctgtc atccgaaatc actttcttcg ctacgataga gccgttcgtt
      361 gtcaagtgaa aatcattacg atggatatgt t
//
LOCUS       DAVBCN010000176          387 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVBCN010000176 DAVBCN010000000
VERSION     DAVBCN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.176"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            147..281
                     /gene="rpmH"
                     /locus_tag="VQ497_002089"
     CDS             147..281
                     /gene="rpmH"
                     /locus_tag="VQ497_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET2518330.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
BASE COUNT          133 a           66 c           83 g          105 t
ORIGIN      
        1 agggcttgca ttcgaaaaag ctggaaacat ttgcctagag ctattgacaa aagtgaaaga
       61 atcagataga atagtaaagt atgtttagta actgatcact ttatagccgg ctaaacgaat
      121 acaaaatcta tgaggaggta ttcatcgtga aacgtactta tcaaccaagt aaacttcgtc
      181 gtgcgcgcaa acacggattc cgtaaccgta tgtcaactaa aaatggtcgt cgcgtattgg
      241 cagctcgtcg tcgtaaagga cgcaaagttt tggctgcata atccaaacga attctatcaa
      301 aaatcagtag gaactcgagt ctactgattt ttatttttgt aaaaaagttc agtagatgca
      361 aatggattcg gaagcgatgt tatagta
//
LOCUS       DAVBCN010000177          372 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVBCN010000177 DAVBCN010000000
VERSION     DAVBCN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.177"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            21..>372
                     /locus_tag="VQ497_002090"
     CDS             21..>372
                     /locus_tag="VQ497_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS1515 family transposase"
                     /protein_id="HET2518331.1"
                     /translation="METVYDKAQKLNSKNFKLLIGVKKETFQLMLEHLNSAYQIQHRK
                     GGRPRSLPMEDQLIMTLRYLRYYPTQRLLAFDFGVGVATVNAIITWVEDTLRASGSFD
                     LDHLEAPSAAVAIDV"
BASE COUNT          106 a           84 c           80 g          102 t
ORIGIN      
        1 aaaagtgtta tactattttt atggaaacag tatacgacaa agcacaaaaa cttaactcaa
       61 aaaacttcaa actattgatt ggtgtcaaaa aggaaacctt tcaactcatg ctagaacacc
      121 tgaattcagc ctatcagatt cagcaccgaa aaggtggacg tccacgtagt ctgcccatgg
      181 aagaccagct cattatgacc ctccgttact tgcgatatta tcccactcag cgtctgctgg
      241 cctttgattt tggcgtcggt gtagctacgg taaatgccat catcacttgg gtggaggata
      301 cacttcgtgc gtcaggtagc tttgatttgg accatttaga agccccgagt gctgctgtgg
      361 ctattgacgt ga
//
LOCUS       DAVBCN010000178          337 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVBCN010000178 DAVBCN010000000
VERSION     DAVBCN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.178"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..>337
                     /locus_tag="VQ497_002091"
     CDS             <1..>337
                     /locus_tag="VQ497_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2518332.1"
                     /translation="FGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLNTKN
                     VTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKHLRIN
                     SGMVILRPKT"
BASE COUNT          132 a           37 c           63 g          105 t
ORIGIN      
        1 gtttggggaa aataaaatat ttgaaagcat tgataactta tttgatatta tagatggtga
       61 taggggcaaa aattatccta aatcagatga gttgtttagt gaggagtact gtttattttt
      121 aaatacaaag aatgttacta aaaacggatt ttcattcgat acaaagcaat ttatcactaa
      181 aacaaaggat aaattacttc gaaaaggcaa gcttgagcgt tatgatatag tcttgacaac
      241 aagaggtact gttggaaatg tagcgtacta cgatgaatta ataaaatata aacatttacg
      301 tataaattca ggtatggtaa tattacgtcc caagaca
//
LOCUS       DAVBCN010000179          336 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVBCN010000179 DAVBCN010000000
VERSION     DAVBCN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.179"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..>336
                     /locus_tag="VQ497_002092"
     CDS             <1..>336
                     /locus_tag="VQ497_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2518333.1"
                     /translation="RLIPNKTAESVNQALTLLLGEHRILSITADNGSEFKRLSEVFPE
                     EHIYYAHAYSSWERGSNENHNRLIRRWLPKGTKKTTPKEVAFIENWINNYPKKCLDYK
                     SPSEFLLGG"
BASE COUNT          109 a           61 c           70 g           96 t
ORIGIN      
        1 taaggttaat tccaaataaa actgctgaat ctgtcaatca ggcgcttacg ttactattag
       61 gggagcatcg tattctgtcc attactgcag ataatggttc ggagttcaaa cgattgtctg
      121 aggtatttcc tgaggaacat atctactacg cacatgctta ctcttcatgg gagagaggtt
      181 caaatgaaaa tcataatcga ttaattcgga gatggttacc taaaggaacc aagaaaacga
      241 ctccgaaaga agtagctttt atcgaaaatt ggattaacaa ctaccctaaa aaatgcttgg
      301 actacaagtc gccaagtgaa tttcttttgg gtggct
//
LOCUS       DAVBCN010000180          326 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVBCN010000180 DAVBCN010000000
VERSION     DAVBCN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.180"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(<1..>326)
                     /locus_tag="VQ497_002093"
     CDS             complement(<1..>326)
                     /locus_tag="VQ497_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2518334.1"
                     /translation="MNQFERKSHEYKAIKRYWKLIQQDSRKLSDKRFYRPTFRMHLTN
                     KEILDKILSYSEDLKHHYQIYQLLLFHFQNKDPEKFFGLIEDNLKQVHPLFQTVFKTF
                     LKNKEK"
BASE COUNT           96 a           48 c           66 g          116 t
ORIGIN      
        1 attttctctt tgttctttag aaaggtttta aagacagtct gaaaaagagg atgaacctgc
       61 ttcagattgt cctcaatgag tccgaaaaat ttctcagggt ctttgttctg aaagtgaaaa
      121 agtaagagtt gatagatctg atagtggtgt ttcaagtctt ctgaatagct taaaatcttg
      181 tcaagaattt ctttatttgt taagtgcatg cgaaaagtag ggcgataaaa acgtttatcg
      241 ctcaatttac gactatcctg ttggatgagt ttccagtaac gcttgatagc cttgtattca
      301 tgagattttc gttcaaactg attcat
//
LOCUS       DAVBCN010000181          304 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVBCN010000181 DAVBCN010000000
VERSION     DAVBCN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.181"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(16..103)
                     /locus_tag="VQ497_002094"
     tRNA            complement(16..103)
                     /locus_tag="VQ497_002094"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67..69),aa:Ser,seq:gct)
     gene            complement(110..183)
                     /locus_tag="VQ497_002095"
     tRNA            complement(110..183)
                     /locus_tag="VQ497_002095"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(147..149),aa:Ile,seq:gat)
     gene            complement(219..289)
                     /locus_tag="VQ497_002096"
     tRNA            complement(219..289)
                     /locus_tag="VQ497_002096"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(255..257),aa:Gly,seq:tcc)
BASE COUNT           95 a           82 c           59 g           68 t
ORIGIN      
        1 aaactctcat taaaacggag aataagggat tcgaaccctt gcgccagtta cccgacctaa
       61 cgatttagca aaccgtcctc ttcagcctct tgagtaattc tccaattaat gggcacgagt
      121 ggactcgaac caccgacctc acgcttatca ggcgtgcgct ctaaccacct gagctacgcg
      181 cccaagttaa aaaacttggt aatttgaaca aagttcaaag cgggtgacga gaatcgaact
      241 cgcgacaaca gcttggaagg ctgtagtttt accactaaac tacacccgca taaatactat
      301 caat
//
LOCUS       DAVBCN010000182          292 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVBCN010000182 DAVBCN010000000
VERSION     DAVBCN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.182"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            51..>292
                     /locus_tag="VQ497_002097"
     rRNA            51..>292
                     /locus_tag="VQ497_002097"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           90 a           54 c           81 g           67 t
ORIGIN      
        1 gaaaaaaaca ataaatctgt cagtgacaga aatgagtgag aactcaaact tttaatgaga
       61 gtttgatcct ggctcaggac gaacgctggc ggcgtgccta atacatgcaa gtagaacgct
      121 gaaggaggag cttgcttctc tggatgagtt gcgaacgggt gagtaacgcg taggtaacct
      181 gcctggtagc gggggataac tattggaaac gatagctaat accgcataag agtagatgtt
      241 gcatgacatt tgcttaaaag gtgcacttgc atcactacca gatggacctg cg
//
LOCUS       DAVBCN010000183          288 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVBCN010000183 DAVBCN010000000
VERSION     DAVBCN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.183"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..>288
                     /locus_tag="VQ497_002098"
     CDS             <1..>288
                     /locus_tag="VQ497_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET2518335.1"
                     /translation="NRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFEW
                     FDEGLYEAPKGYTLEDLLATVKYYVEHPNERPHSDNGFGNASDHVQRNKNG"
BASE COUNT           93 a           59 c           59 g           77 t
ORIGIN      
        1 caaccgcgtg aaagcagcta agaaggtgcc acttgatcgt atgccttaca atcttcaata
       61 tactgtagaa gtcaaaaacg gtagtttaat catacctcat tatgaccatt accataacat
      121 caaatttgag tggtttgacg aaggccttta tgaggcacct aaggggtata ctcttgagga
      181 tcttttggcg actgtcaagt actatgtcga acatccaaac gaacgtccgc attcagataa
      241 tggttttggt aacgctagcg accatgttca aagaaacaaa aatggtca
//
LOCUS       DAVBCN010000184          284 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVBCN010000184 DAVBCN010000000
VERSION     DAVBCN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.184"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
BASE COUNT          118 a           30 c           51 g           85 t
ORIGIN      
        1 ttagaacaga aaaaatgctt ggagtatttg tttgtgtgtt tacttttata taacaaacta
       61 taaacacaac aaaaatataa aaaaagagac aaaaaagaac agaaagtaat taacaacgat
      121 ttcatatagt gttatactta tagcataaag ttaatggaaa gaagggaaaa ccttatggta
      181 ttagattatt tctttgacaa aaaccttgtg ttttgcttag aagcggataa tcaagaataa
      241 ctctttgatc aggtagcaag cttgttggag gaacgagaaa ttgt
//
LOCUS       DAVBCN010000185          277 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVBCN010000185 DAVBCN010000000
VERSION     DAVBCN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.185"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..>277
                     /locus_tag="VQ497_002099"
     CDS             <1..>277
                     /locus_tag="VQ497_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2518336.1"
                     /translation="QRIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAM
                     QGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQG"
BASE COUNT          126 a           37 c           47 g           67 t
ORIGIN      
        1 aacaacgcat aatagaagca atcgaatcag ctttagaaaa agtagatgaa tatgctgaaa
       61 gttataatag actagaacag ctagataaag aatttccaga taaactaaaa aaatctattc
      121 ttcaatatgc tatgcaagga aaattagttg aacaagaccc aaatgatgaa tcagtcgaag
      181 ttttacttga aaaaatacga gcagaaaaac aaaaactctt tgaagaaggc aagattaaaa
      241 agaaagattt ggacatttct attgtttccc aaggaga
//
LOCUS       DAVBCN010000186          274 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVBCN010000186 DAVBCN010000000
VERSION     DAVBCN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.186"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
BASE COUNT           87 a           49 c           50 g           88 t
ORIGIN      
        1 ccagagcttg ctcttactat tatagcagat gttatagctg gaattatctt gtatttcgtc
       61 tgcaaatggc tagatggtaa gaagtagacc gactgactag cctataaaca cccgttaaat
      121 cgctaagata cgtcaaaaaa gcccttaact atggcactag ttaggggctt tggtgttcta
      181 atgaacctta tacactaact acattctagc atataagccc agatatttca agagttttat
      241 ttattgttta aagttctgaa aggtctataa tgaa
//
LOCUS       DAVBCN010000187          272 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVBCN010000187 DAVBCN010000000
VERSION     DAVBCN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.187"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            complement(1..272)
                     /locus_tag="VQ497_002100"
                     /pseudo
     CDS             complement(1..272)
                     /locus_tag="VQ497_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT           75 a           72 c           48 g           77 t
ORIGIN      
        1 atacttgagg ctgtattcaa ttgcccttcc tagttttgaa cccgataaaa ctgactgacg
       61 ccggcaccaa gcaaagaaat cttccattag gttggagctc ttcttgacgt ttctgtagtc
      121 gttcatcagc tggcaaagcc tcccagtctc tttccaagaa aaataactga tcacaataag
      181 ctaaaccttt agctcctaag gatgatttat ccgcttgctt gggggtcgct tcaaaaaact
      241 tccttctcac atgcgcccaa catccaacaa gt
//
LOCUS       DAVBCN010000188          270 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVBCN010000188 DAVBCN010000000
VERSION     DAVBCN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.188"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..>270
                     /locus_tag="VQ497_002101"
     rRNA            <1..>270
                     /locus_tag="VQ497_002101"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           69 a           74 c           72 g           55 t
ORIGIN      
        1 tgccggtaat aaaccggagg aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc
       61 tgggctacac acgtgctaca atggctggta caacgagtcg caagccggtg acggcaagct
      121 aatctcttaa agccagtctc agttcggatt gtaggctgca actcgcctac atgaagtcgg
      181 aatcgctagt aatcgcggat cagcacgccg cggtgaatac gttcccgggc cttgtacaca
      241 ccgcccgtca caccacgaga gtttgtaaca
//
LOCUS       DAVBCN010000189          251 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVBCN010000189 DAVBCN010000000
VERSION     DAVBCN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.189"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
BASE COUNT           80 a           43 c           48 g           80 t
ORIGIN      
        1 ctcttactat tatagcggat gttatagctg gaattatctt gtatttcgtc tgcagatggc
       61 tagatagtaa ggagtagacc gagtgactag cctataaaca cccgttaaat cgctaagata
      121 cgtcaaaaaa gcccttaact acggccatag ttaggggctt tggtgttcta atgaacctta
      181 tcaattaact acattttagc atataatcta caatagttca agcattaagt gaggttgatg
      241 atctaaattt a
//
LOCUS       DAVBCN010000190          244 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVBCN010000190 DAVBCN010000000
VERSION     DAVBCN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.190"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
BASE COUNT           69 a           32 c           58 g           85 t
ORIGIN      
        1 gattctctga agttatggat gtcgttccaa tatgtgtacg ttggaatgtt agtgcttata
       61 tggggggaac agaatcctct cttgattgaa gacaagttag ccattaggct ggtttgtctc
      121 tttgtcaact gtagtgggtt gatataatag tattagtgag tgggataaaa gtttcattta
      181 gtttattcag tacaaattta acgggtcaag atttattgaa ctgcacccca atagttagac
      241 agaa
//
LOCUS       DAVBCN010000191          229 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVBCN010000191 DAVBCN010000000
VERSION     DAVBCN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.191"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            <1..>229
                     /locus_tag="VQ497_002102"
     CDS             <1..>229
                     /locus_tag="VQ497_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2518337.1"
                     /translation="YTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHS
                     HWIKKDSLSEAERAAAQAYAKEKGLTPPSTDH"
BASE COUNT           72 a           49 c           55 g           53 t
ORIGIN      
        1 tacactgatg atgagattca agtagccaag ttggcaggca agtacacaac agaagacggt
       61 tatatctttg atcctcgtga tataaccagt gatgaggggg atgcctatgt aactccacat
      121 atgacccata gccactggat taaaaaagat agtttgtctg aagctgagag agcggcagcc
      181 caggcttatg ctaaagagaa aggtttgacc cctccttcga cagaccatc
//
LOCUS       DAVBCN010000192          208 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain CTD0664
            SAMEA1919811-rid20177923.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVBCN010000192 DAVBCN010000000
VERSION     DAVBCN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919811
            Sequence Read Archive: ERR298096
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980261.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:42:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,103
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 40
            rRNAs                             :: 4, 4 (16S, 23S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 53 of 101
            Pseudo Genes (incomplete)         :: 46 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 22 of 101
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919811-rid20177923.denovo.192"
                     /strain="CTD0664"
                     /db_xref="taxon:1313"
     gene            81..154
                     /locus_tag="VQ497_002103"
     tRNA            81..154
                     /locus_tag="VQ497_002103"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:115..117,aa:Met,seq:cat)
BASE COUNT           49 a           49 c           60 g           50 t
ORIGIN      
        1 aagggaacgg tcttgaaaac cgtcaggcgt gtaaaagcgt gcgtgggttc gaatcccaca
       61 tcctcctttt atattattaa cgcgggatgg agcagctcgg tagctcgtcg ggctcataac
      121 ccgaaggtcg taggttcaaa tcctgctccc gcaataaggc tcggtagctc agttggtaga
      181 gcaatggatt gaagctccat gtgtcggc
//
